LOCUS       DASUMG010000001         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_803152, whole genome shotgun sequence.
ACCESSION   DASUMG010000001 DASUMG010000000
VERSION     DASUMG010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803152"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VFX46_00005"
     CDS             complement(<1..1642)
                     /locus_tag="VFX46_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX5599000.1"
                     /translation="MTEAASTLGQWHFWIDRGGTFTDVIGRDPEGRLHALKVLSENPG
                     AYEDAALEGIRRMLGVKSNDPIPSERIASVKMGTTVATNALLERKGEPTVLVITKGLK
                     DQLEIGYQARPDIFAKKIIKPEMLYTRVIEANERVRADGTIETPLDLAALRQDLERAK
                     ADGIGAVAIVFMHAYAFPEHEQQAAALAREIGFTQVSVSHEISPLIKIVGRGDTTVAD
                     AYLSPILRRYVEKVADALGARNQENRRAPKILFMASSGGLKSADLFQGRDAILSGPAG
                     GIVGMAETARIAGFDKVIGFDMGGTSTDVSHFAGDYERAFETEVAGVRLRVPMMRIHT
                     VAAGGGSILHFDGSRFRVGPDSAGSNPGPKCYRRGGPLTVTDANLMVGKLSASFFPKI
                     FGPNRDEPLDEEAVRAAFTELAEQIGDGRTPEEVADGFLRIAVENMANAIKKISVQRG
                     YDVTEYVLNCFGSAGGQHACLIADTLGMETVMIHPLSGLLSAYGMGLAKIRASREQSV
                     EAPLEAASMQDVEALSEKLRAEVIAEVEAEGVSADEIST"
     gene            1749..>2039
                     /locus_tag="VFX46_00010"
     CDS             1749..>2039
                     /locus_tag="VFX46_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5599001.1"
                     /translation="MLKHMTLGLLAAGLCVAPAMAETKWDMPTPYPDNNFHTKNVRQF
                     AEDVEKATNGQLKIVVHSAGSLIKHPEIKKSVRQGIAPIGEILQSLAANENPI"
BASE COUNT          394 a          628 c          624 g          393 t
ORIGIN      
        1 tcgtcgagat ttcgtcagcc gaaacgccct cagcctcgac ctcggcgatc acctcggcac
       61 gaagcttttc ggacaaagcc tctacgtcct gcatcgatgc ggcttcgagc ggcgcctcga
      121 cggactgctc tcggctcgcg cggattttgg cgagccccat accgtaggct gagagaaggc
      181 ccgacagggg atggatcatc accgtttcca tgccgagcgt atcggcgatg aggcaggcgt
      241 gctggccgcc ggcggagccg aaacagttca gcacgtactc ggtcacgtcg tatccgcgct
      301 gcacggaaat ttttttgatg gcgttcgcca tgttctcgac ggcgatgcgg aggaatccgt
      361 cggcgacctc ctcgggcgtg cggccgtcgc caatctgctc agcgagctcg gtgaacgcgg
      421 ctcgcacggc ttcctcgtcg agcggctcat cgcgattggg gccgaagatc ttcggaaaaa
      481 agctggccga cagcttgccg accatgaggt tggcgtccgt cacggtcagc ggaccgccgc
      541 ggcggtagca tttcggcccc ggattggagc cggcgctgtc ggggccaacg cggaacctcg
      601 acccgtcgaa atggaggatg gaaccgccgc cagcggcgac cgtatggatg cgcatcatcg
      661 gcacgcggag acgcacacca gcgacctcgg tttcgaaggc acgctcgtag tctccagcga
      721 agtgcgaaac gtccgtggac gtaccgccca tgtcgaagcc gataacctta tcgaatccgg
      781 cgatacgtgc cgtctcggcc atcccgacga ttccgccggc cggcccggag agaattgcgt
      841 cgcggccttg gaacaggtcg gcggatttga ggccgcccga ggaggccatg aaaaggattt
      901 tgggtgcgcg gcggttctcc tggttcctgg cgccgagggc gtcggcaact ttctcgacat
      961 agcgccgcag aatgggcgac agataagcat cagccaccgt cgtgtcgcca cggcccacga
     1021 tcttgatgag cggcgagatt tcgtggctca ctgaaacctg tgtaaacccg atttcgcgcg
     1081 ccaacgctgc agcttgctgc tcatgctcgg ggaacgcgta ggcgtgcatg aacacgatcg
     1141 cgacggcgcc aatcccatcc gctttcgcgc gttcgaggtc ctgtcgcagc gccgccaggt
     1201 cgagcggtgt ctcgatggtg ccatccgctc tcacacgctc gttggcctcg atgacgcgcg
     1261 tgtagagcat ttcgggcttg atgatctttt tcgcgaagat atcgggccgc gcctgatagc
     1321 cgatctcgag ctgatccttc aggcccttgg tgatgacgag gacggtcggc tcacccttgc
     1381 gctcaagtaa cgcattcgtg gcaacggtgg tgcccatctt aacggaggcg atcctttccg
     1441 acggaatggg atcgttcgat ttgacgccga gcatccgccg gattccttcc agcgcagcgt
     1501 cctcgtaggc gccgggattc tctgagagga ccttcaatgc atgcaggcga ccttcgggat
     1561 cgcggccgat gacgtcggtg aacgtgcctc cccgatcaat ccagaaatgc cactgtccga
     1621 gagtgctcgc cgcttctgtc atcggtttcc ccgtcgccta attcgaataa acgcggtaaa
     1681 aggaaatctg ccgagggccg tatgcccacg ttccgtctaa tggctacacg agggaggtcg
     1741 agtaaacgat gctgaaacat atgactctgg ggctgctcgc cgcaggcctc tgcgttgccc
     1801 ccgcaatggc cgaaacaaaa tgggacatgc cgacccccta tcccgataac aacttccata
     1861 cgaagaatgt caggcagttc gccgaggacg tggagaaggc gacgaacggc cagctcaaga
     1921 tcgtggtgca ctcggctggt tcccttataa agcatccaga gatcaagaag tcagtgcgcc
     1981 aagggattgc gccgatcggc gaaattctcc aatccctcgc tgccaacgag aacccgatt
//
LOCUS       DASUMG010000002         3882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_80482, whole genome shotgun sequence.
ACCESSION   DASUMG010000002 DASUMG010000000
VERSION     DASUMG010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80482"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            358..1440
                     /locus_tag="VFX46_00015"
     CDS             358..1440
                     /locus_tag="VFX46_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5599002.1"
                     /translation="MVWLDKAVEFDVLSLSNPNRVVVSLPDVKMRLPNAADDLETGLV
                     KSFRVGLSAPGRSRIVIDVRAPVVVKRSEIVKRERGHHLVLDLAPAPSALQAGKSRAA
                     KLQTVQPPLPRPASRPEEIIARAYKPVIVIDPGHGGHDSGAKKNGVVEKDVVLAFSLI
                     LRDKLEASGRYRVVLTRDSDVFISLDERRAIAERNKAALFIAVHADYAGAHARGATIY
                     SLRDTTARALQRSASSEVVNAVLTSQELSGVERSDARAIRSILADLAEREIAVTKERT
                     NTFTRSVIEFMSVSTEMRPNADREANFRVLKTAKVPSVLIELAYVTNPQDARNLKSNA
                     WRNKVADSIVTAIDNYFSYQIARLPM"
     gene            complement(2278..3207)
                     /gene="rpoH"
                     /locus_tag="VFX46_00020"
     CDS             complement(2278..3207)
                     /gene="rpoH"
                     /locus_tag="VFX46_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEX5599003.1"
                     /translation="MVTKSLPTVANGSMGLARYLEEIRRFPMLEPDEEYMLAKRWQEH
                     ADAEAAEKLITSHLRLVARIAMGYRGYGLPIGEIISEGNVGLMQAVKRFDPDRGFRLA
                     TYAMWWIRAAIQEYILRSWSLVKMGTTAAQKKLFFNLRRAKGQIQALEEGDLRPDQVK
                     AIAKRLGVPEEDVISMNRRLAGDASLNAPVRADLDSGEWQDWLVDDSPDQEEELIERD
                     EMDHRRAFLESALETLNERERRIFEARRLSDEPMTLEDLSAEFGVSRERIRQIEVRAF
                     EKVQKAVQQAAKSSEASAAAAAAGAAGAAGASA"
BASE COUNT          733 a         1210 c         1116 g          823 t
ORIGIN      
        1 actgatcgtt ccgcctcagc ccatcgccgc agcggatggg ctgatggtcg tccgttcatt
       61 gatcagtaac ctttcccgca tcataagatt acctcggagg gatcagcaaa aagccacgat
      121 tgggcttcat cagaagccac cgtgttcagt tgtcagtcct ccgcgttccg gagtttgcgt
      181 acaatgagtg taagcagagt acggaccgtt tcggctgccg cgttatggat ccgggctgcc
      241 gttgtgggta ccgttctgct ccttttcgcc agctttatta cactcgtctc tccggcgcct
      301 gtttccgccg cacccacagg gccaaccgcg gctgcgaacc tacctacgac ccgttttgtg
      361 gtctggctcg ataaggcggt tgagttcgac gtcctatcac tgagcaatcc gaaccgcgtc
      421 gtcgtatcgc taccggatgt gaagatgcgg cttcccaacg ccgctgatga cctcgaaacg
      481 gggctcgtga aatcatttcg cgtcggactt tcggcgcccg gacgctcccg tatcgtcatc
      541 gacgtgcgcg caccggttgt ggtgaagcgt tcggaaatcg tcaaacggga gcgtggacac
      601 catctcgtgt tggatctcgc gccggctcct tcagccctgc aggcggggaa atccagggct
      661 gctaagctcc agacggtgca gccgccattg ccgcgcccag cctcacggcc ggaggagatc
      721 atcgcacgtg cctataagcc cgttattgtg atcgatcctg gtcatggtgg ccacgattcc
      781 ggggccaaga agaacggtgt cgtcgaaaag gacgtcgtcc tcgcattcag ccttattttg
      841 cgagataagc tcgaggccag tggccgctac cgcgttgtct tgacacggga ttctgatgtc
      901 ttcatctcgt tggatgagcg tcgtgcaatt gccgaacgca acaaggcggc tctctttatt
      961 gcggttcacg cggactacgc cggcgctcat gcgcgtggcg ccaccatcta ttctcttagg
     1021 gacactactg cgcgagcatt gcaacgctcc gccagctccg aggtggtcaa cgccgtgctc
     1081 accagccagg agttgtcagg cgtcgaacgg agcgacgccc gcgccatcag gtccatcctg
     1141 gccgatcttg cagagagaga aatcgccgtc accaaggagc gcaccaatac cttcacacgc
     1201 tcagtcatcg aattcatgag cgtctctact gagatgcgac cgaatgcgga ccgcgaggcg
     1261 aatttccggg tcttgaagac ggcgaaggtt ccttcagtgc tgatcgagct tgcttacgtg
     1321 acgaaccctc aggatgctcg caatctcaag tctaacgctt ggcggaacaa ggtcgccgac
     1381 tcgatcgtca cggccataga caattacttt tcgtatcaga tcgcgcggct accgatgtga
     1441 tctgacagag ttgccacggg ggaagacggc gcagaaggcc ggcccgagtt cgcttgggtc
     1501 ggccttcatt catctagccg gggtcaaggc atggtgtgca tctgactatg tggtcctgaa
     1561 gctccgatca cactcgtacg gaacgcacgt gatgccagcc cggcagctgc ggagttagac
     1621 gatacttggt gggtgcacag gtttaagtcg ccacagaagc acacgacacc actcatccgc
     1681 tcgtccagca gagctactgg tctccaactc cttccaccag tacttgcgac gacgagaccc
     1741 cagacccccc gatttgggaa cggaccgtgg atgaaggcgc ttcgacatgg gttgctccat
     1801 cgtcttattg cagtgccgca tccacacgcc atcgaagccg cgcaccggcc ccttccgcga
     1861 gcgcattgcg catgcgtccg cccggtaccc ccgtcagctt caagtcgtga tgatgcagcg
     1921 gaaaaggaca ggagcgcgaa gcctcgcgct gggatgtatg gctggcgctg cgggcggcgg
     1981 tcctgtcctt ctccgcctcg cgccagccgg cagtcgtgaa ggtgccggtt cctattgtga
     2041 cgttcaggcc gcaagcgagt gccgtgagct ctgacgcgca ccttcctcgg cggcccgggg
     2101 tcagcttcgc cggaccgctg cgaggtcgat cacctcacag gtgaattgta agcgattcgt
     2161 gcctaagcct gtgcaaaaac ctgctcaaaa gaaaaccgac cgcaagtgcg gccggtttca
     2221 atgtcatctg aggcctgctt ttcggagtca gccgatcgca atgcctgtca ggtagcgtta
     2281 ggccgacgca cctgcagcac cagccgctcc ggcggcagcg gcagcagcgc tcgcttcgct
     2341 ggacttcgcg gcttgctgca ccgccttctg caccttctcg aaagcgcgca cttcgatctg
     2401 gcggatccgt tcgcgcgaca caccgaactc cgccgacaag tcctcgagcg tcataggctc
     2461 gtccgacagg cggcgagcct cgaagatgcg gcgctcgcgc tcatttagcg tctccaatgc
     2521 cgattcgagg aaggcgcgcc ggtgatccat ttcgtcccgc tcgatgagct cctcctcctg
     2581 atcgggagag tcgtctacga gccagtcctg ccactcgccg gaatcaaggt cggcacgcac
     2641 gggggcgttc agcgatgcat cgccagccag gcggcgattc atcgagatga cgtcctcttc
     2701 cggcacgccc agccgcttgg caatggcctt cacctgatcg ggccggaggt cgccctcctc
     2761 cagagcctgg atctgacctt tcgcgcgccg cagattgaag aagagcttct tctgggcggc
     2821 tgtggtgccc atcttcacaa ggctccagga gcgcaggatg tactcctgaa tagcggcgcg
     2881 gatccaccac atcgcatagg tggccagacg gaagcctcga tcggggtcga accgcttgac
     2941 cgcctgcatg aggccaacgt tgccctcgga gatgatttca ccgatcggca ggccatagcc
     3001 cctatagccc atggcgatac gcgccacgag ccgcaggtgc gacgtgatca gtttctcggc
     3061 ggcctccgcg tcggcatgct cttgccagcg cttggcgagc atgtactcct cgtcgggctc
     3121 aagcatggga aagcgccgga tctcctcgag ataccgggca agacccatgg acccatttgc
     3181 gactgtcggc agcgatttcg tgaccatctc agtttcagcc gagtatcaaa ccactatatt
     3241 cggcggtgaa actcggttgc ctctttccga ccaactcttt agcttcgccc agtagcgtga
     3301 ccagagcaag gcgctatggc caagctatag ttccgcacaa gttttgtcat tgtgcgatgt
     3361 ccttaactca cggtcacact acggtgattt tcgacggaac ggtgttcggg gattggagtt
     3421 ccactgccct ccacttatat aagtaggctt cattattttt gcagacccgt cgttatccgt
     3481 ttttgcatcc cattaacatt gctggttccg ctcctgagcg cattgcgcag cgtcaataaa
     3541 tctggcggga gaggactctc gaaccgcagg cgttcccctg tccgcggatg ctcgaacgcc
     3601 agaagcgctg cgtgaagagc ctgtcgattc aaagccttca aggcggatct cgcttctgga
     3661 ttcagcctcc gctcgcttgc ggcgaagcct gcaccgtaag tcgtgtctcc caggagaggg
     3721 tggccgacat gcgccagatg aacccggatc tggtgcgtcc gtcccgtctc caacgtaact
     3781 cttaaacggc tgacagcgat ccttccgtcg gtgccggggt aagtttcgtc gatccgccaa
     3841 tgggtcacgg cgcgacggcc ttgtccttct cggagaaccg cg
//
LOCUS       DASUMG010000003         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1044242, whole genome shotgun sequence.
ACCESSION   DASUMG010000003 DASUMG010000000
VERSION     DASUMG010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044242"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFX46_00025"
     CDS             <1..1247
                     /locus_tag="VFX46_00025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX5599004.1"
                     /translation="IRDTEIENLLNDYARPIFKAAGLGEGRVAVRIVRSDIFNAFVLD
                     GRNVFIHTGALMQAETPNQIIGVIAHEAGHIAGGHLAALRSRIARDQTRALLIRMLGL
                     GVAIATGAGEAVFAGDELIMRSLLAERRSQEAAADQAGLRYLNATRQSGRGMLETFER
                     FAQQEYVSDKYKDPFVRSHPVASDRLARLRELVESSPYYHAKDPPELQLRHDMMRAKL
                     AGYLERPQVVFNRYPRSDTSLPARYARAIATFMQGGQNALPAALQQVDALIRERPDNP
                     YFYELKADFLTRSGKSREAIAPLREALRLSKGASLIRVQLASALLATNDPKVVNEAAD
                     LLRKAIVEDENPRAYRSLADAYYRLGRQAEADAAIAQAYFLEGDVKQAQNFATRAQRA
                     LRQGSPTWLKMDDIINYKSSDL"
     gene            1310..2182
                     /locus_tag="VFX46_00030"
     CDS             1310..2182
                     /locus_tag="VFX46_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5599005.1"
                     /translation="MPAKLVTRLMNALTTKKGGSIAAAVLIAGGLTVLAGAPALSTAS
                     NLQLAQAGGSQQPPATPNTSGSFSAEQKQAIEQIIKDYLLRNPEILVEVSRELEKRQA
                     AQQAAEQKRVLLENRDKIFSSSADFVLGNPNGDITVVEFFDYNCPYCKRALPELMELV
                     KSDSNIKVVFKEFPILGEGSVSAAKAAMASLRQGKYLEFHMALMQARQVNEDNLFKIA
                     ERVGLDVDRLKKDMESPEIDAAIKQTSDIAQELGITGTPVFIVDATVNVGLLRHEELS
                     QLVNEARKAGCQVC"
     gene            2312..2767
                     /gene="aroQ"
                     /locus_tag="VFX46_00035"
     CDS             2312..2767
                     /gene="aroQ"
                     /locus_tag="VFX46_00035"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX5599006.1"
                     /translation="MAKPVYVLNGPNLNMLGLREPAIYGAETLADVQRRVESRAAQLG
                     LTVDFRQSNFEGELVTWVQEAREKASGIILNAGAYTHTSIALLDALNAAELPVIEVHL
                     SNIFRRESFRHHSYISLAARGVICGFGSKGYELALDAMADILSRTSNQA"
     gene            2818..3300
                     /gene="accB"
                     /locus_tag="VFX46_00040"
     CDS             2818..3300
                     /gene="accB"
                     /locus_tag="VFX46_00040"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX5599007.1"
                     /translation="MAKDQKERGNSEQQLIRELAALLVETGLSEIEIEKAGLKIRVAR
                     NLTVSAAAPPQPAPAPSASPSEPAQPAAPAEAAIDFANHPGCVKSPMVGTAYRAPEPG
                     APPFVEVGSRVTQGQTLMIIEAMKTMNHIPAPRAGTVTHILVENGQPVEYGEPLVIIE
                     "
BASE COUNT          709 a         1025 c         1020 g          605 t
ORIGIN      
        1 tcatccgcga caccgaaatc gaaaacctgc ttaacgatta cgcccgaccg atcttcaaag
       61 cagcggggct cggcgagggc cgcgtggcag tccgtatcgt ccgtagcgat atcttcaacg
      121 cgttcgtgct ggatggccgg aatgtgttca tccacaccgg cgccctcatg caggccgaga
      181 cgcccaatca gatcatcgga gtgattgccc acgaggctgg ccacatcgcg ggcgggcacc
      241 tggcggcgct gcgctccaga atcgcgcggg atcagacacg cgccttgctg atccgcatgc
      301 tgggcctagg cgttgcgatc gcaaccgggg ccggtgaggc ggtcttcgct ggcgacgagc
      361 tcatcatgcg ctcgctgctc gccgagcggc gttcgcaaga ggctgctgcc gaccaagcag
      421 gcctgcggta cctcaacgca acgcgccagt cgggccgcgg catgcttgag acattcgagc
      481 gtttcgcaca gcaggagtac gtctctgaca agtacaagga tccgttcgtg cgaagccacc
      541 ccgtcgcaag cgacaggttg gctcgtttgc gagagctcgt cgaaagcagt ccttactacc
      601 acgcgaagga cccgccggag cttcagctcc ggcatgacat gatgcgagcc aagctggcgg
      661 gctacttgga gcggccgcag gttgtgttca accgctatcc acggtccgac acatcgctac
      721 ccgcgcggta cgcgcgcgcc atagccacgt tcatgcaggg tggtcagaat gctctgccgg
      781 cggcacttca gcaggtcgat gcccttattc gggaacggcc tgacaatcct tacttctatg
      841 agttgaaggc cgatttcctc actcgctccg gaaagagccg cgaagctatc gctccgttgc
      901 gtgaggcgct tcggctctcc aaaggagcga gcctcattcg ggtccagctt gcatccgctc
      961 tcctggccac caacgatcca aaggtcgtta acgaggcagc agatctgcta cggaaggcga
     1021 tcgtcgagga cgagaacccg cgagcctacc gctcactcgc cgacgcctac tatcggcttg
     1081 gccgccaggc agaggcagat gcggcgattg cgcaggcgta tttccttgaa ggggacgtga
     1141 agcaggctca aaacttcgct actcgtgctc aacgcgctct gcgccagggc tcgccgacct
     1201 ggctgaagat ggacgatatc atcaactaca aatccagtga tctctagggc gaagccaaag
     1261 ctgggcgagc ttggagcgct ggagaacaga tacaaggaac ccgcctctca tgcctgcaaa
     1321 actcgtgacc cgtttgatga atgccctcac gacaaagaag ggcggatcta ttgccgcggc
     1381 agttctgatt gccggagggc tgacggtcct ggccggcgct cccgcgttgt cgactgcctc
     1441 caatctgcag ctcgctcaag caggcgggtc gcagcaaccg ccggcaacgc caaacacctc
     1501 gggatcgttc tcggccgagc agaagcaggc gattgagcaa atcatcaagg attacctgct
     1561 ccgcaaccct gaaattctcg ttgaggtcag ccgcgagctt gagaagcggc aggccgcgca
     1621 gcaggcggcc gagcaaaagc gcgttctcct ggagaacagg gataagattt tttcgtcgtc
     1681 agccgacttc gttctcggca atccgaacgg cgacatcaca gtcgtcgagt tcttcgatta
     1741 caactgccct tattgtaagc gagctcttcc tgaactgatg gagctggtga agtccgactc
     1801 caacatcaaa gtggtcttca aggagttccc catccttggc gaaggctcag tatccgccgc
     1861 caaagctgca atggcatcgc tacggcaagg aaagtatctg gagttccata tggcgctgat
     1921 gcaggcgcgt caggtcaatg aggacaacct cttcaagatt gccgagcgtg tcgggctgga
     1981 cgtcgaccga ttgaagaagg acatggagag ccccgaaatc gacgccgcta tcaagcaaac
     2041 gtcagacatt gcccaggagc tcggcatcac aggcacgccg gtcttcatcg tcgacgccac
     2101 ggtgaacgtc ggcctgctcc gacatgaaga attgagccag ctcgtgaacg aggcgcggaa
     2161 ggcaggctgc caagtctgtt gaactgccgt cctcgcaagc cagcgcggct cgcggcgtgc
     2221 ttccgccgat acgtgaaagc tgataaacgg accgcgacct gccggtttac cggcgcgagc
     2281 ggtggcgcga gcaatttcga ttgacatcac aatggcgaag cccgtctacg tcctcaatgg
     2341 acccaacttg aacatgctcg gcttgcgcga gccggccatc tatggcgcgg aaacgctggc
     2401 cgacgtgcaa cgccgcgttg agtcgcgggc agcacagctc gggcttacgg tcgatttccg
     2461 gcagtcgaac ttcgagggtg agctcgtaac ctgggttcag gaagcgcggg agaaggcgag
     2521 cgggatcatc ttgaatgcgg gggcgtatac ccatacttcg atcgccctgc tcgacgcgct
     2581 caacgcagcc gagttaccgg ttatcgaggt gcacctgtcg aatatcttcc ggcgcgagag
     2641 tttccgccac cactcctata tttccttggc tgcgcgcggc gtgatctgcg gctttggctc
     2701 aaagggttac gagctggcgc tcgatgccat ggcggacatt ctgagccgga cgtcaaacca
     2761 agcgtaattc agagctggcg cgggagcggc gatttgaagg cggtggaatc gaaggaaatg
     2821 gcaaaagacc aaaaagaacg gggcaactcc gagcagcagc tgatccgcga gcttgcggcg
     2881 ctgctcgtcg agacgggatt gagcgagatc gaaatcgaga aggccggtct taagatccgc
     2941 gttgcccgca atctgacagt gtcagcggcc gcgccgccgc aaccggcacc ggccccgtct
     3001 gcgagcccgt ccgagcccgc tcagccggcg gcgcccgccg aagccgccat tgacttcgcc
     3061 aatcaccctg gctgcgtgaa atcgccgatg gtcggcaccg cttatagggc gccagaacca
     3121 ggcgcaccgc cctttgtcga ggtcggctcg agggtcacgc aggggcagac gctgatgatc
     3181 atcgaggcga tgaaaactat gaaccatatc cctgcgccgc gggccgggac ggtgactcac
     3241 atcctcgtgg aaaatggtca gccggtcgaa tacggcgaac ccctcgtcat catcgaatag
     3301 cgacttggga agagccgagc gcacgcagat tccctctccg ccaaaccgcc tggataaga
//
LOCUS       DASUMG010000004         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_198, whole genome shotgun sequence.
ACCESSION   DASUMG010000004 DASUMG010000000
VERSION     DASUMG010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..980
                     /locus_tag="VFX46_00045"
                     /pseudo
     CDS             279..980
                     /locus_tag="VFX46_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321940.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1100..1690
                     /gene="rsmD"
                     /locus_tag="VFX46_00050"
     CDS             1100..1690
                     /gene="rsmD"
                     /locus_tag="VFX46_00050"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5599008.1"
                     /translation="MRVIGGRFRGRPLASPRDPSDLRLRPSSDRVRESVFNILAHGIP
                     EFSLEGAKVLDLFAGTGAFGVEALSRGASFCLFVEQDAEARGLIRRNVEAFGLAGVTR
                     IFRRDATDLGPAGHRDGFTLVFADPPYGRGLAEKALASAAEGGWLADGAIAVIEEADR
                     AELALPAGFKEIDRRTWGSTQVVFARYETQSGARAP"
     gene            1704..2678
                     /locus_tag="VFX46_00055"
     CDS             1704..2678
                     /locus_tag="VFX46_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599009.1"
                     /translation="MQKPSPPHPDPQAERISGWIEAGLYVLTIAILSVSNAFAISQGA
                     HPIVFILYSLVISAVGMLIYTGPGNEPIRVMLNPLSWFVGLGIIGVEVSFCLILLTLS
                     PAEANIILRLSIPLALLLGWIIFRRSPGTGAWIGAAIVAAGVGSLLFTVDLSTQAIGL
                     FYGVIAAASICLRGFASEFHPWNRAARSVFEKIRITGLVVLVTGLAGLFLVGLAATLV
                     GLGFAERSNLLPAPADLWHWPTIVLALFVGSLIFATMSYLQFSSVVKIETENFIATSA
                     FMPLAAALVQSLAVSIGLIDAVVFDWRLLPGSILIIAGVMLLIATRRS"
     gene            complement(2684..>3262)
                     /locus_tag="VFX46_00060"
     CDS             complement(2684..>3262)
                     /locus_tag="VFX46_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HEX5599010.1"
                     /translation="RPLGAARCQIHETYIIAQTRNSMIIVDQHAAHERLVYERMKRML
                     ADGGVVRQGLLIPEIVELSEDEAEALAERADELAELGLVLERFGPKAVAVRETPALLG
                     QTDVKGLVRDLAAEILRDEGRQALKERLEAVCATMACHGSVRAGRRLTADEMNALLRE
                     MEATPFAGQCNHGRPTYVELQLSDIERLFGRR"
BASE COUNT          546 a         1066 c         1022 g          628 t
ORIGIN      
        1 gggcacctcg ccgcgatctg ccgcacgccg ggcctcatcc agagcgatga gcatgtggct
       61 tctattggag gtcggtgcca taagtccacc ttcaaaaccg gatcgatctc atcatgcgtg
      121 agccccgaat gggccgacca aaacagcgcc taaaaggcca acgccgcgac tcggctaaac
      181 ggcatacgac cgcgcagcgc gccccgcagc agggccgcga cggcacttac atgcctcaac
      241 cccagcagcc ggcctccggc cgaccgccgc aaacgatgcg catcgccaag gcgatcgcca
      301 gagcaggtct gtgttcccgg cgggaagccg aacgctggat cgccgagggg cgagtaagcg
      361 tcaatggcga agtcctgaaa agccctgcgc gcgacgttgg gcctggcgac gtggtgcttg
      421 tcgacggcaa gcccttgccg tccttcgagc ccgtacagct ttggcggtac tacaagccgc
      481 ggggcctcgt caccacgcac cgcgatccac aggggcgtcc gacggtgttc gagaacctgc
      541 ccgagggtat gccgcgggtc atttccgtcg gccggcttga cttcaatacg gaaggcctgc
      601 tcctactgac gacggacggc gccttggcac gacacctcga gctgccgtcg acaggctggc
      661 tccggcgtta ccgcgtgcgc gctcacggaa aggtcacgca ggccgatctc gacaagctga
      721 aggatggtat cgaggtggag ggaatccgct atggcccgat cgaggccacg ctcgatcgcg
      781 tgcagggcag caacgtctgg atgacgatcg cgctgcggga gggcaagcac cgcgaggtgc
      841 gcaaggtgct cggtgcgctg ggactggaag tcaatcgcct gatcagagtc tccttcggtc
      901 cgtttcaact tctcgatttg aagccgggtg aggtcgagcc agtccggcgg cgtgtattga
      961 tcgaccagct tggcccccgg accgcgcaac ttctgggact cactgctgac ggtgaggagg
     1021 agaaggcccg ccgtcggcgc cgcaagtctg cggcatccga acccgaggcg cgcctagagg
     1081 acttcgagga cgacggacga tgagggtcat cggaggccgg tttcgtggcc ggccgctagc
     1141 ctcccccagg gatccgagtg acctgcggct gcggccgtcg tcggaccgcg tgcgtgagtc
     1201 cgttttcaac atcctcgctc atggaatccc tgaattctca ctcgaaggcg ccaaggtgct
     1261 cgaccttttc gctggcacag gcgcgttcgg cgtcgaagcc ctgtcgcgcg gtgccagctt
     1321 ctgcctgttc gtggaacagg atgcggaggc gcgcggcctg atccgccgca atgtcgaagc
     1381 ctttggcctg gctggcgtaa cgcggatctt tcgacgggac gccaccgacc tcggcccggc
     1441 gggacatcgc gacgggttca cgttggtctt cgccgatccc ccgtacggaa gaggactcgc
     1501 cgagaaggcg ctcgcctctg cagcggaagg cggctggctg gccgacggcg ctatcgcggt
     1561 gatcgaggaa gctgatcgag ccgaacttgc gctgcccgca ggctttaaag agatcgatcg
     1621 acgtacgtgg ggtagcacgc aagttgtctt cgcccgctac gagacccaat ccggcgcgcg
     1681 tgcgccctga acgactggaa gctatgcaga aaccatcacc cccacacccg gatcctcagg
     1741 cagagcgcat cagcggctgg attgaggccg ggctttatgt gctcaccatc gccatcctga
     1801 gtgtctcgaa cgcattcgcg atatcacaag gcgctcaccc gatagttttc atcctctact
     1861 cgcttgtgat ctccgccgtc ggaatgttga tctacacggg ccccggcaac gagcccatcc
     1921 gtgtgatgtt gaatccattg agctggtttg tcgggctcgg gatcatcggg gtcgaggttt
     1981 ccttctgcct catcctgctc acgctctcgc cagccgaagc caacatcatc ctgcgcctat
     2041 ccattccgtt ggcgctcctt ctcggctgga tcatattccg gcggagccct ggtacggggg
     2101 cgtggatcgg cgccgccatc gtcgccgcgg gtgtcggatc actgcttttc acagtcgatc
     2161 tttcgaccca ggcaatcggc ctattctacg gtgtcattgc cgcagcatcg atctgcttgc
     2221 gcggatttgc gagcgagttt catccctgga accgggctgc ccgaagtgtc ttcgagaaaa
     2281 tccggatcac cggcctggtg gttctcgtga ccggcctcgc gggcctgttt ctcgtcggtt
     2341 tggcagccac gctggtcggt ctcggctttg cagaacgaag caaccttctg ccggctcctg
     2401 ctgacctttg gcattggccg acgatcgtgc tggccttgtt cgtcggctcg ctgatcttcg
     2461 ccaccatgag ctatctgcag ttctcctcgg tggtgaagat agagaccgag aacttcatcg
     2521 ccaccagcgc tttcatgccg ctcgccgcag cgctcgtgca gagcctggcg gtgtcgattg
     2581 ggctcattga tgccgtcgtt ttcgactggc ggctcttgcc tggaagcatt ctgattatcg
     2641 ccggcgtgat gctgctgatc gcaacccgga ggagttgacc gcctcagcgc cggccgaaga
     2701 gccgctcgat gtcgctgagt tgcagctcca catacgttgg ccggccgtgg ttgcactggc
     2761 cagcgaatgg agtcgcctcc atctcccgga gcagcgcgtt catctcgtcc gccgtgaggc
     2821 gccgcccagc gcggacgctg ccgtggcagg ccatcgtggc gcacaccgct tcgagccgct
     2881 ctttcagcgc ctgccgtccc tcgtcccgca ggatctcggc ggcaaggtcg cggaccagcc
     2941 ccttgacatc ggtctggccg agtagagcgg gcgtctcccg cacggccaca gctttggggc
     3001 cgaaccgctc cagcacgaga ccgagctccg ccaattcatc cgcacgctcc gccaatgcct
     3061 ccgcctcgtc ctcgctcagc tcaacgatct caggaataag gaggccctgg cgcaccacgc
     3121 cgccgtcggc gagcatcctt ttcatgcgct cgtagacgag ccgttcgtgc gcagcgtgct
     3181 ggtcgacaat gatcatggaa ttgcgcgttt gcgcgatgat gtaggtctcg tggatctggc
     3241 aacgcgccgc cccaagcggg cg
//
LOCUS       DASUMG010000005         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_482113, whole genome shotgun sequence.
ACCESSION   DASUMG010000005 DASUMG010000000
VERSION     DASUMG010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482113"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3027)
                     /locus_tag="VFX46_00065"
     CDS             complement(<1..3027)
                     /locus_tag="VFX46_00065"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX5599011.1"
                     /translation="MRNPDGSVVFKLDGIQVPKTWSQVAADILAQKYFRKAGVPRRLK
                     RVEETQVPSWLWRSVPDEAALAELPENERYGGETDARQVFDRLAGTWTYWGWKGGYFT
                     TEDDARAFFDELRYMLAMQMAAPNSPQWFNTGLHWAYGIDGPSQGHYYVDHETGRLMQ
                     STSAYEHPQPHACFIQSIEDDLVNENGIMDLWVREARLFKYGSGTGTNFSKLRGANEP
                     LSGGGRSSGLMSFLKIGDRAAGAIKSGGTTRRAAKMVVVDVDHPDIEEFINWKVREEQ
                     KVAALVTGSKICQKRLKAIMKACVNCEAEGDACFDPAKNPALKRAIKEARRDQVPASY
                     IARVIQFARQGFKDIDFETYDTDWDSEAYLTVSGQNSNNSVRVTDEFLRAVLEDKEWA
                     LTARTTGEVVKTVRARDLWDQIGYAAWACADPGIQFHTTINDWHTCPQSGPIRASNPC
                     SEYMFLDDTACNLASLNLMMFRAPNADVKRGVLFDTDSFEHACRLWTIVLEISVLMAQ
                     FPSRKIAELSYRYRTLGLGFANIGGFLMASGIPYDSPEGRAICGAIAAIMTGVAYATS
                     AEMARELGPFPGYFKNADDMLRVIRNHRRAAYGEKDGYERLSTLPVPLDHASCPDAGL
                     IEAAKRAWDRALALGEAHGYRNAQATVIAPTGTIGLVMDCDTTGIEPDFALVKFKKLA
                     GGGYFKIINRSVPLALRALGYSETEIAEIEAYAVGHGSLRNAPAINHATLKAKGFTDE
                     ALQKIEEALATAFDIKFVFNRWTLGDKFLTETLKIPAEKLNEPNFDLLTHLGFSKKDI
                     EAANEYACGAMTLEGAPHLKPEHLPVFDCANPCGRRGKRFLSVESHIRMMAAAQPFIS
                     GAISKTINMPNDATVEDCKQAYLLSWRLGLKANALYRDGSKLSQPLNAQLLGDDTDEQ
                     EEVAEQLTSDKPMAARSQVVAERIVERVVERARGREKLPARRKGYTQKAIVGGHKVYL
                     RTGEYEDGRLGEIFIDMHKEGAAFRSLMNNFAIAVSL"
BASE COUNT          502 a          988 c          981 g          575 t
ORIGIN      
        1 aaggctcacc gcgatggcga agttgttcat cagcgaccgg aaggcagcgc cctccttgtg
       61 catgtcgatg aagatttcgc cgagacggcc atcctcgtac tcgcccgtgc gcaggtagac
      121 cttgtgcccg cccacgatcg ccttttgcgt gtagcccttg cgccgcgccg gcagcttctc
      181 gcgcccacgg gcacgctcga cgacccgctc gacgatccgc tcagccacca cctgcgagcg
      241 cgctgccatc ggcttgtcgg aggtgagctg ctcggcgacc tcctcctgct cgtcggtatc
      301 gtcgccgaga agctgggcat tgagcggttg cgacaacttc gagccgtcgc ggtagagcgc
      361 gttcgccttg agacccagcc gccatgacag caggtaggcc tgcttgcagt cctcgaccgt
      421 cgcatcgttc ggcatgttga tggtcttgga gatcgcgccc gagatgaacg gctgcgccgc
      481 cgccatcatg cggatgtggc tctccaccga caggaaccgc ttgccgcgac gcccgcacgg
      541 gttggcgcaa tcgaagaccg gcaaatgctc cggcttcaga tgcggcgcgc cttccagcgt
      601 catggccccg caggcgtact cattcgcggc ctcaatgtcc ttcttcgaga agccgaggtg
      661 cgtgagcaga tcgaagttgg gctcgttgag cttttcagcc ggaatcttga gcgtttccgt
      721 caggaacttg tcgcccaacg tccagcggtt gaagacgaac ttgatgtcga atgccgtcgc
      781 aagtgcctct tcgatcttct gcaaggcctc gtccgtgaag cccttcgctt tcagcgtcgc
      841 gtggttgatg gccggagcgt tgcgcagtga tccgtggccg accgcgtagg cttcgatctc
      901 cgcgatctca gtctcactgt agccgagcgc gcgcagggcc aacgggaccg agcggttgat
      961 gatcttgaag tatccgccgc ccgccagctt cttgaacttc accagggcga agtccggctc
     1021 gatgcccgtg gtgtcgcaat ccatcaccag gccgatggtg cccgtcggcg caatgacggt
     1081 cgcctgggca ttgcgatagc cgtgcgcctc acccagcgcc agcgcccgat cccaggcccg
     1141 tttcgcggct tcgatcaacc cggcatccgg gcaagaggca tggtccagcg gcaccggcag
     1201 cgtcgaaagc cgctcgtagc catccttctc gccgtaggcc gcgcggcggt ggttgcggat
     1261 cacgcgcagc atgtcgtcgg cgttcttgaa gtagccgggg aaaggcccga gctccctcgc
     1321 catctccgct gacgtcgcat aggcgacgcc cgtcatgatc gccgcgatcg caccgcagat
     1381 ggcgcgcccc tcgggcgagt cgtaaggaat gcccgacgcc ataaggaagc cgccgatgtt
     1441 ggcgaagccc aggcccagcg tgcggtagcg gtaggaaagc tccgcgatct tccggctcgg
     1501 gaattgagcc atcagcacgg agatctcgag cacgatggtc cagaggcggc aggcgtgctc
     1561 gaacgagtcg gtgtcgaaga gaacgccgcg ttttacgtcg gcattcggtg cccggaacat
     1621 catcaagttg agcgacgcca ggttgcaggc ggtgtcgtcg aggaacatgt actccgagca
     1681 cggattggac gcgcggatcg ggccggactg cgggcaggtg tgccaatcgt tgatggtggt
     1741 gtggaactgg atgcccgggt cggcgcaggc ccacgcggcg tagccgatct ggtcccacag
     1801 gtcgcgggct ctgacggttt tcacgacctc gcccgttgtg cgcgccgtaa gggcccactc
     1861 cttatcctcc agcacggcgc ggaggaactc atcggtcaca cggacagagt tgttggagtt
     1921 ttggcctgaa accgtaaggt aggcctcgct gtcccaatcg gtgtcgtaag tctcgaaatc
     1981 aatgtccttg aagccctggc gcgcgaactg gatcacgcgg gcgatgtagc tcgccggcac
     2041 ctggtcgcgc cgtgcctcct tgatggcgcg cttcagcgcc gggttcttcg cgggatcgaa
     2101 gcaggcgtca ccctcggcct cgcagtttac gcaggccttc ataatggcct tgagccgctt
     2161 ctggcagatc ttggagccgg tgacgagagc ggcgaccttc tgctcctcgc ggaccttcca
     2221 gttgatgaac tcctcgatgt ccgggtggtc gacgtcgacc acgaccatct tggcagcgcg
     2281 ccgcgtggtg ccgcccgact tgatggcgcc ggccgcgcga tcgccgatct tgaggaagct
     2341 catgagaccg gaggaccgtc cgccgccgga caatggctca ttcgccccgc gcagcttgga
     2401 gaagttggtg cccgtgcccg agccgtattt gaagagccgc gcctcgcgta cccaaaggtc
     2461 catgatcccg ttctcgttca cgagatcgtc ctcgatggac tgaatgaagc acgcgtgcgg
     2521 ctgcggatgc tcgtaggccg aggtcgactg catcagccgg ccggtctcgt ggtcgacgta
     2581 gtagtgcccc tggctcggcc cgtcgatgcc ataggcccaa tgcaggcccg tgttgaacca
     2641 ctgcgggctg ttcggcgcgg ccatttgcat ggccagcatg tagcggagct cgtcgaagaa
     2701 ggcgcgggcg tcgtcctcag tggtgaagta gccgcctttc cagccccagt aggtccacgt
     2761 gccggcaagc cgatcgaaga cctgccgcgc gtccgtctcg ccaccgtaac gctcattctc
     2821 gggcagctca gcgagcgcgg cctcatccgg cacggaccgc cagagccatg acgggacctg
     2881 agtttcctcg acgcgcttca ggcgccgcgg cacgccggcc ttgcggaagt acttctgcgc
     2941 cagaatgtcg gccgcgacct ggctccaggt cttgggcacc tggatgccgt cgagcttgaa
     3001 gaccaccgaa ccatcggggt tgcgaatctc gctggtgacc gaggcg
//
LOCUS       DASUMG010000006         3612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_883771, whole genome shotgun sequence.
ACCESSION   DASUMG010000006 DASUMG010000000
VERSION     DASUMG010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883771"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="VFX46_00070"
     CDS             complement(<1..1120)
                     /locus_tag="VFX46_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5599012.1"
                     /translation="MTYRAPVDDIMLVLKTAAGLPDLIERGIVDNLDEETARAIIEEA
                     GKFGSEVLDPLNAVGDRVGSRLVDGRVVTPPGWKEAYDAFVAGGWSALPCPPEYGGQG
                     LPQIISTVACEIWNSANLSFGLCPLLTQGAVDALNAHGSEELKRIYLPKMVSGEWTGT
                     MNLTEPHAGSDLGALTTRAERQPDGTYRITGTKIFITYGDHELTENIIHMVLARLPDA
                     PPGTKGISLFLVPKRLVNPDGTLGERNDIVCASLEHKLGINASPTCVLKYGENGGAVG
                     YLVGEENRGLATMFIMMNQARLAVGVQGVALAERATQKAAAYAAERRQGRRVRDGQGM
                     VPIIEHPDIRRTLMTMRSMTQAARAICLATAREIDIAHR"
     gene            1205..2170
                     /locus_tag="VFX46_00075"
     CDS             1205..2170
                     /locus_tag="VFX46_00075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX5599013.1"
                     /translation="MPVVKVSEDAIARAAAELRAGNLVAFPTETVYGLGADATNGRAV
                     ALIFEAKGRPRFNPLIVHVSSRAEAERFVRFTDKASRLADAFWPGALTLVLPRRSDSN
                     ISELVSSGLDTIAVRVPSHPIAQALLKAAGVPVAAPSANISGHVSPTTAQHVEADLGD
                     KVALILDGGPTEHGLESTVVDVTGEQIVLLRPGAVTREAIEEVLGEPVLKAGESSAQG
                     DRIRSPGQLASHYAPRAKVRLNATDLHPGEALLAFGEPLPGAEEHMMENLSPTGDLVE
                     AAANLFAALRRLDATGVETIAVMPIPETGLGEAINDRLRRAAAER"
     gene            complement(2971..3603)
                     /gene="hisG"
                     /locus_tag="VFX46_00080"
                     /pseudo
     CDS             complement(2971..3603)
                     /gene="hisG"
                     /locus_tag="VFX46_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
BASE COUNT          653 a         1207 c         1093 g          659 t
ORIGIN      
        1 cgcgatgagc aatgtcgatc tcccgcgccg ttgcgaggca gatcgcccgc gccgcctgcg
       61 tcattgatcg cattgtcatc agggtgcggc ggatgtccgg gtgctcgatg atcggcacca
      121 tcccctggcc atcgcgcacc ctgcggccct ggcggcgctc ggcggcatat gccgcggcct
      181 tctgggtcgc gcgctcggcc agagccacgc cctggacgcc gaccgccagc cgcgcctggt
      241 tcatcatgat gaacatggtc gcgaggccgc ggttctcctc gccgacaagg taaccgactg
      301 cgcccccgtt ctcgccgtat ttcagcacgc aggtcgggct cgcattgatg ccgagcttgt
      361 gctccagact ggcgcagaca atgtcattgc gctcgcccag cgtcccatct gggttcacca
      421 gccgtttcgg aacgagaaac agcgaaattc ccttggttcc gggcggagca tccggcaggc
      481 gcgcgaggac catgtgaatg atgttctcgg tcagctcgtg gtcgccgtag gtgatgaaga
      541 tcttcgtgcc ggtgatgcgg taggtgccgt cgggctgccg ctccgcccgc gtcgtcagcg
      601 caccgagatc agagccggca tgcggctccg tgaggttcat ggtgccggtc cattcgcccg
      661 aaaccatctt cgggaggtaa atgcgcttca gctcctcgga gccatgcgcg ttgagtgcgt
      721 ccacggcacc ttgcgtgagc agcgggcata gcccgaagga aagattggcc gagttccaga
      781 tttcgcaggc cactgtcgag atgatctggg gcaggccctg gccgccatac tcaggagggc
      841 atggcaatgc gctccagccg cccgccacga acgcgtcgta ggcttccttc cagccgggcg
      901 gcgtcacgac tcttccatcg acaagccgcg agccgacgcg atcgccgaca gcgttgagcg
      961 ggtccagcac ctcgctgcca aacttgcccg cctcctcgat aatggcgcgc gccgtttcct
     1021 cgtcgagatt gtccacgatg ccacgctcga tgagatccgg caggccggcc gccgtcttga
     1081 gtacgagcat gatgtcatct acgggagccc tgtacgtcat ccagtcatcc ttgttcggtg
     1141 gaggtctcgt aggtttaggg gtaagggact gatcctaaaa gcgcaatcga agggcttcac
     1201 cgtaatgcct gtcgtcaagg tgagcgaaga cgcgatcgcc cgcgcagctg cggagctccg
     1261 ggccgggaac ctcgtcgcct tccccaccga aaccgtttac ggcctcgggg ccgatgctac
     1321 caacggacgt gccgtcgctt tgatcttcga agcaaaaggc cgcccgcggt ttaacccact
     1381 aattgtgcac gtcagcagcc gagcagaggc ggagcgtttc gtccgtttca cggacaaggc
     1441 atcccgactg gcggatgcat tttggcctgg cgcactgacg ctcgttttgc cgcggcgttc
     1501 ggattccaac atttcggagc tggtgagctc tggcctcgat acgattgccg tcagggtgcc
     1561 ctcccatccc atcgcccagg cgttgctcaa ggcagccgga gtgccggtcg ccgccccttc
     1621 cgccaacatc tctggtcacg tcagcccgac gacggcccag catgtcgagg ccgatctcgg
     1681 cgacaaggtc gccctgatcc ttgatggagg accgaccgag cacgggctcg agtcgaccgt
     1741 ggtcgatgtc accggcgagc agatcgtcct gctgcggcca ggcgccgtca cgcgagaggc
     1801 catcgaggaa gtgctcggtg agccggtcct caaagccggc gaatcatcag cgcagggtga
     1861 ccggatccga tcgcccggcc agctcgccag ccactatgcg ccgcgcgcca aagtgcgcct
     1921 caacgcgacg gacctccatc ccggcgaagc cctgctggcg tttggggagc ccctccccgg
     1981 cgcagaagaa cacatgatgg aaaatctgag ccccacggga gacctcgtcg aggcggcagc
     2041 caatttgttc gcagccctgc gccggctcga tgccacgggc gttgaaacca tcgcggtaat
     2101 gccgattccc gagacagggc tgggcgaagc catcaacgac cggctccgcc gcgctgcggc
     2161 ggagcgataa ccgatatatc cggcctcccg cttggagcgg gggagatcct tcgcgcatca
     2221 gaaaaaagga aggccgcacc ccaagtcgga tgcgaccttc gtgagctgca gggcgagttg
     2281 ccccgccctc ctccactccc aagcagctca cctcttcgcg cagtcctcaa gctgctgatt
     2341 gcgcaactgg gcctgctttt tccaaacatc gggagcgacg ccgcggcacc attccatgtg
     2401 agctgtaaga tcaaaccgcc actctggccc cgtgaaccca cactttcgct gctcgttctc
     2461 ctgctgctgc ttcagggcca ttttcgcgta ccactcacaa ttggcttgtg ccgttgcctt
     2521 aggaccgagc cacagcgtgg taagcagcaa ggcgcctaga aggacggcac atatccgaag
     2581 catcgacatt ctcccacctc gcctttcgac ccgcctgggc agcttctccg tcaccacccg
     2641 aaacatctgg gcttggcaag atcttgccta acgcttatcc cggacggcat gaacccatcc
     2701 tgaacgcgcg gcagatcctc cgagtgagga cggatccctt ccgagcaagc agtacgacac
     2761 gcgcgcgagc ccgccaagtc cgtggagagg cagcgttgtt agagggcccc ggatttgcgc
     2821 ccggcccgca acacgcggtg gcctggataa cgccggtatt caatgactag ggaaggtcga
     2881 agcgggcatt gggaccgcgg gagggaattt gcgatccgat caccccctca acgccgccaa
     2941 aatccgatct cgcgcagcaa tagccgtttg ggaccactgc gcgcggcgcg aggcgataag
     3001 gtgcgcctcg gagcgcagga taagcccatc gtccaagacc ttgagcccgt tggccttcag
     3061 ggtcgcgccc gttgtcgtga tatcgacgat cagatcggcc gcaccggatg cgggcgtgcc
     3121 ttccgtggcc ccgagactct cgacgatgcg gtagtcagtg acccccttcg cctgaaaaaa
     3181 tcgccgtgtc agattggtgt acttggtggc cacgcgcagc cgccgttcat gagtcctgcg
     3241 gaagaccgcg gccatcgctt gcaagtgcgc catcttccgg atgtcgatcc agcatgcggg
     3301 caccgcaacc acgacgtccg cccggccgaa acctagcggc aacagcagct ccacgcgctc
     3361 atcggcgacg atgatgtgct cgcggatcaa atcctcaccc gtgatgccga ggtgcacatg
     3421 cccggacttc aaataatgcg cgatttccga cgcggaaacg aacgctacct caacgccctc
     3481 cagcccttcg atctcgccgc ggtatccgcg gttgctgttc gtctttcgca gcttgagccc
     3541 tgcgttaccg agcgcttcgg ccgcctgctc catcagtcgg cccttggagg ggacggcgaa
     3601 aatgagattg tc
//
LOCUS       DASUMG010000007         4717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_482487, whole genome shotgun sequence.
ACCESSION   DASUMG010000007 DASUMG010000000
VERSION     DASUMG010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482487"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VFX46_00085"
     CDS             <1..1472
                     /locus_tag="VFX46_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599014.1"
                     /translation="TDERVEAAAREARTIEALEARITAQLPKVRVTYQPGAAGKIRIG
                     GKAVDDGTVLTPSRPLTLEIEGIGAITIDPAVSESVEADKANLQTRRSALADLLAAMD
                     VPDLAEARKRLDERHRLERELAQAADRLAMRAPDGLKALSAEVERLRELAGSETVGSD
                     TVVPERSEIEGEIERLSFALRDVEQKIETLVQEQAGLREQLAQLNAAAQARQQRLEHL
                     ARVLPPAEERSRRRAELEAEVAAAQQNLNEAVRELTAWREKAPDDAAMRALEAELTEA
                     RRQQQSAAEEIARVEKELAVLEAQIERDRQDGLSMKVADLEERVAALNARVQHFEREL
                     AAIDLLLETISDVEQQSRELYFKPILQRLEPYLNCVFPGAKVGLGPNFSVEAVIRGDV
                     AEQLGSLSDGTQEQIAVLARLAFARLLADAGQPTPLILDDALVYSDDSRIEALFEVLR
                     RAAEVHQVIIFTCRSRTFEQLGGTRLSLGPWSPDAALAA"
     gene            complement(1510..1968)
                     /locus_tag="VFX46_00090"
     CDS             complement(1510..1968)
                     /locus_tag="VFX46_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5599015.1"
                     /translation="MLPSFSNKRPERDEAGFPTPSSSSALGRSNERASLSIVGPDLVI
                     VGDLVSNGQVQVDGEIQGDIYGTHVVIGDGARIVGGIVSEEVVVRGTVTGSIRCRKVM
                     LQAESKVEGDIYHKSLGIEEGAYFEGRSRRVDDPLAGVQRPVPASADSSS"
     gene            complement(2150..2614)
                     /locus_tag="VFX46_00095"
     CDS             complement(2150..2614)
                     /locus_tag="VFX46_00095"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5599016.1"
                     /translation="MVQENDLAPDFELPTEDGIVRLSELRGKPVVVYFYPKDDTSGCT
                     AEAKDFDALRRAFEEAGAVVVGISPDSVESHAKFRKKHGLGLRLASDPDKTAAQAYGV
                     WVEKSMYGRKYMGVERSTFLIDANGRIAKVWRKVKVPGHAQAVLQAVRELSS"
     gene            2843..>4717
                     /locus_tag="VFX46_00100"
     CDS             2843..>4717
                     /locus_tag="VFX46_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599017.1"
                     /translation="MSRRPGAEAGPVRLMSQFRSFGPHGQRIVGVARALAKPCWVCAK
                     YLLPLLLLLFIAGGIFDVRILFGPISLKMLASPIARSIAAELPGLGVSVEDALVRLNE
                     EGELEFRLRNVRLLDADGSPIAVTPLAALSPSFRALRSGRVAPDEVVLIEPRLLLTYT
                     AEHGLSFSFTRPGGSSDPAQSQVAAEAKAESSATSDRDGDPGAGLPTALRQLDLARVI
                     AEASARARQGDDATSFLRKIGLRNATLIFEQGGEQRVWRVPQADFAFSHKKTRSLMAG
                     LVTVDSGRGPWTFEFSSEASEATRQVMLRAAVRDVVPSTVADALPPLAPLRALSLPAS
                     GDVTLQLTSQGELIGGSFSLDLARGEIVLPWFGDLPLMMEGGNLDLKYDPNERALLIE
                     PSTIAWGRSRVTIAGKVSSSIEPDGGEVWTYALASVEGRLAPEDGKSLPIEIESWRAE
                     GTYRPASGAFTTEQTLKAGGGELHLSGEADPRVSGVRVEGRAGTVPIATIMSLWPRAL
                     GSNARTLLRESISGGQILAGSLRFVKGPDKAGRNGAERLSIAFQTTDIVVNHRGLPKL
                     EVPSALIRLEGPTLEVTVPKAAVTLASGRKIDLSAGRFTAVDILSEYPIAQIAFRSES
                     S"
BASE COUNT          871 a         1467 c         1524 g          855 t
ORIGIN      
        1 tcaccgatga gcgcgtcgag gcggctgccc gggaagcgcg caccatagag gcgctcgaag
       61 cgcggatcac tgcgcaactg ccgaaggtgc gggtcaccta tcagcctggc gcggccggta
      121 agatccgcat cggtggcaaa gccgttgacg atggcaccgt cctcaccccg tcccggccgc
      181 ttaccctcga aatcgaaggc atcggcgcca tcaccatcga tccggctgtt tcggagagcg
      241 tcgaggctga caaggctaat ttgcagaccc gccgttctgc gctcgctgat ttgctggccg
      301 ccatggatgt cccggatctg gcggaagcgc gtaagcgtct cgacgagcgg caccgcttgg
      361 agcgagagct cgctcaggca gcggatcggc tcgcaatgcg agcacccgac gggctcaagg
      421 cactttctgc ggaagtggag cggttgcggg agctggcagg cagcgagact gttggaagcg
      481 ataccgttgt accagagcgt tcggagatcg aaggagagat cgaacgcttg agcttcgcgc
      541 tccgcgatgt agaacagaaa atcgagaccc tagtccagga gcaggccggg ctgcgggagc
      601 agctggcaca actcaacgcg gcagcgcagg cgcgccaaca gcggcttgag caccttgcca
      661 gagtcctacc gccggccgag gaacggtcgc gtcggcgagc tgaattggaa gccgaggtcg
      721 ctgcagcaca gcagaacctg aacgaggcgg tccgcgagct gacggcatgg cgggagaagg
      781 cgccggacga tgcggccatg cgggctctgg aagcagagct gacagaggcc agacggcagc
      841 agcagagtgc ggccgaagag attgctcgcg tcgaaaaaga gcttgccgtt ctcgaggcgc
      901 agatcgaacg ggatcgtcag gacggcctct caatgaaggt ggccgacctc gaggagaggg
      961 tggctgcgct gaatgctcgt gtacaacact ttgaacgaga gctggccgcc atagacctgc
     1021 ttctcgagac catctcagac gtcgagcaac agtcgcgtga gctttacttc aagccgatcc
     1081 tgcagcggct cgagccttac ctgaattgcg tcttcccggg cgcgaaggtt ggccttgggc
     1141 ccaacttctc agtcgaagcc gttatccgcg gcgatgttgc cgagcagctc ggatcgctca
     1201 gcgacggtac gcaggagcag atcgccgtgc tggcgcggct ggcattcgca aggctcctgg
     1261 cagatgcggg gcagccgacc ccgctgatcc tcgacgacgc cctagtctac tccgacgaca
     1321 gccggatcga ggcgcttttc gaagtgctcc ggcgcgccgc cgaagtccat caggtgatca
     1381 tcttcacgtg tcgatcgcga acattcgagc agctgggcgg cacacgcctg tccctggggc
     1441 cgtggagccc cgatgcggcg ctcgcagctt gaagtggacg ctcgcagtgt ttcggacagt
     1501 acgagggttc taggaggagc tgtcggctga cgcaggcacc gggcgctgaa ccccggccag
     1561 cggatcatcg acacgccggg accgaccctc gaaataggcg ccttcctcga tgccaagcga
     1621 cttgtggtag atgtccccct cgaccttgct ctcggcttgc agcatcacct tccggcaccg
     1681 gatggagccg gtgaccgtgc cgcgcaccac cacctcttcg gagacaatgc cgccgacgat
     1741 gcgggcgcca tcaccgatga cgacgtgcgt tccgtaaatg tcgccctgaa tttcgccgtc
     1801 cacttggact tggccattcg agacgagatc gccgacgatg acgaggtcag ggccaacgat
     1861 ggagaggctt gcacgctcat tgcttcgacc gagcgccgat gaggaagaag gggttggaaa
     1921 accggcctca tcccgctcag gcctcttgtt cgaaaaactg ggtaacataa tacgcacctc
     1981 cactcgctcg cgtcatgccc ctcgacgggc gagcgggcca gcacatcctc gcatgtgata
     2041 gccactcccc ccgagctgcg gactttgttc aatgcagcac gcgcgaaacc tagcacgtga
     2101 caatgcgatg gtcacggcca tggttggccg tggcggcagg cgaagcggat caggagctca
     2161 gctcgcggac tgcctggagc accgcctggg catggcccgg caccttgacc ttgcgccaga
     2221 ctttggcgat gcgcccgttc gcatcaatga gaaaggttga acgctccacg cccatgtact
     2281 ttcgcccgta catggatttt tccacccaga ccccgtaggc ctgcgcagcc gtcttgtccg
     2341 ggtccgatgc caggcgcaac cccaatccat gcttcttgcg aaacttggcg tggctctcga
     2401 cgctgtcagg cgagattccg acgaccactg ctcccgcctc ctcgaatgct ctgcgcaggg
     2461 cgtcaaagtc ttttgcctcg gccgtgcacc ccgacgtgtc gtcctttggg tagaagtaga
     2521 cgacgacagg cttgccacgc agctcggata gtcggactat gccatcctcc gtcggcagct
     2581 cgaaatcggg cgccaagtca ttctcttgaa ccatgggggg tgcctttctt tcgtcgcttt
     2641 aggctcgcac atcgttcgta ggccaacgta ctaacattgc agtaccgcaa ccagtgattc
     2701 tcggtcagag ttgccttgct ggtcagccgc cgcacgattc gctgcacgcc ggctacggtc
     2761 gcgtatgtcc tctaggccgc aggcaaggcg ggcctgaaag tcattgctaa tgacacgacg
     2821 tctactgacg agaggagagc gcgtgagcag gaggccgggc gcagaagcag gccccgtgcg
     2881 gctgatgtct cagttccgca gcttcggtcc gcatggccaa cgcatcgttg gcgtcgcgcg
     2941 cgctttggcg aagccatgct gggtgtgcgc aaagtatctt ttgccgctcc tgctgttgct
     3001 tttcatcgcc ggcggcatct tcgacgtgcg cattctcttt ggcccgatct ccctcaagat
     3061 gctggcgtcg ccgatcgcac gcagcattgc ggcagagctc cccgggttgg gcgtcagtgt
     3121 ggaggatgct ctcgtccggc tgaacgagga gggcgaactc gaattccggc tgcggaacgt
     3181 gcgtctgctc gacgcagatg gaagcccgat cgccgttacg ccgctggcgg cgctttcacc
     3241 cagcttcagg gcgcttcggt ccggccgtgt cgcgcccgat gaggtggttc tcatcgaacc
     3301 tcggctgctg ctgacttata cggccgagca cggactttcg ttcagcttca cgcggccggg
     3361 cgggtccagt gatccggccc agtcgcaagt cgccgctgaa gcgaaggcgg agtcttccgc
     3421 gacctcggac cgggacggcg accccggcgc cggacttccc actgcgttgc ggcagctcga
     3481 tctcgcccgg gtgatcgcgg aggcatcagc ccgagccagg cagggcgacg acgcaacatc
     3541 gttcttgcgc aagatcggcc tgcgcaacgc gacgttgatc ttcgagcagg ggggtgagca
     3601 gagggtctgg agggttcctc aggctgattt cgccttctcg cacaagaaga cccgaagttt
     3661 gatggcaggt ctcgtcaccg tcgactccgg cagaggccct tggacattcg aattctcctc
     3721 cgaagcctct gaagcgaccc ggcaggtgat gttgagagcg gccgttcgcg acgttgttcc
     3781 aagcacggtc gccgatgcgc tgccgccgct ggcacccctg cgagcgttga gcctccctgc
     3841 aagtggggac gtgacgctac aactcacgtc tcagggtgag ctgatcgggg gtagtttctc
     3901 cctcgacctg gcccgggggg aaatcgtgtt gccttggttc ggggatctgc cgctcatgat
     3961 ggagggcggc aatctcgatc tgaagtacga tcccaatgaa cgggcgctgc tgatcgaacc
     4021 ttcgacgatc gcttggggac gcagtcgggt aacgatcgca ggcaaggtgt cgagctccat
     4081 cgagccggac ggtggcgagg tttggacata cgcgctggca agcgttgaag gccgcctggc
     4141 gcccgaggat ggcaagagcc tgccgatcga gatcgagagc tggcgcgcag aaggaaccta
     4201 tcggccggca agcggtgcgt tcacaacgga gcagacgctc aaagccgggg gcggcgagct
     4261 gcacctttcg ggcgaggctg acccacgggt ctctggtgtc cgcgtcgagg gccgagccgg
     4321 cacggtcccg atcgcgacga tcatgagcct ctggccccgt gcgctaggaa gcaacgcgcg
     4381 gacgcttctc cgcgagagca tctcaggagg acagatcctc gctggaagct tgcggttcgt
     4441 gaaggggccg gacaaggctg ggcgaaacgg agcggagcgt ctttcgatcg cctttcagac
     4501 cacggatatt gttgtcaacc atcgcggtct ccccaagctc gaggtcccaa gcgcgctcat
     4561 tcgcctcgag ggcccgacgc tggaggtcac ggtgcccaaa gccgcggtca cgctcgcaag
     4621 cggacgcaaa atcgacctca gtgccggccg atttactgcg gtcgacattc tgtccgaata
     4681 tccaatcgcg cagattgcgt tccgcagcga aagctcc
//
LOCUS       DASUMG010000008         5239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_884286, whole genome shotgun sequence.
ACCESSION   DASUMG010000008 DASUMG010000000
VERSION     DASUMG010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884286"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VFX46_00105"
     CDS             <1..687
                     /locus_tag="VFX46_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate polymerase"
                     /protein_id="HEX5599018.1"
                     /translation="LFINESQLVFLEDMMKMQGFLGNYQMAGAFQLLRSNDLIWSRIV
                     HDYLMGERQPMFDLMAWNADTTRMPYRMHSEYLRKLFLENDLAAGRYVADGRPIALTD
                     IRVPVFAVGTETDHVAPWRSVYKVHLLLDTQVTFVLTSGGHNAGVVSEPGHPRRRYRV
                     STKEEDDLYVDADTWFEQTPVKQGSWWPEWAAWLSDRSGDWTSPPPMGAPEKGYRPIL
                     DAPGSYVLQP"
     gene            705..1688
                     /locus_tag="VFX46_00110"
     CDS             705..1688
                     /locus_tag="VFX46_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEX5599019.1"
                     /translation="MRAMVLEKPGTELKLLDVPVPKPGPGEVLVRVAACGVCRTDLHV
                     VDGDLPDPKLPIIPGHEIVGYVTELGEGVEKFKEGDRVGIPWLGRTCGQCRYCLSGRE
                     NLCESPGFTGYTIDGGYAEFTVAHADYCFPLPMDYDDASAAPLLCAGLIGYRSLKLGG
                     PGQALGIYGFGAAAHIVAQVANFERRRLFAFTRPGDTAAQDFARSLGAEWAGGSDEAP
                     PVPLDVAIIFAPVGELVPAALKAMVKGGTVVCGGIHMSDIPSFPYELLWHERVVRSVA
                     NLTRDDATEFLELAPQVPVKTTIETFPLEDANEALARLREGRLTGAAVLVP"
     gene            1790..2083
                     /locus_tag="VFX46_00115"
     CDS             1790..2083
                     /locus_tag="VFX46_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599020.1"
                     /translation="MLTIEDMIAFSGLSRDEVLAIAEHENLPETAACALAEYLMQQEH
                     GPERIREMIIEDIRAAQQRGDKEHVQILLHVLHHFVRAHPESLPISGKQTDIT"
     gene            complement(2369..2569)
                     /locus_tag="VFX46_00120"
     CDS             complement(2369..2569)
                     /locus_tag="VFX46_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599021.1"
                     /translation="MNPVAHRITPDLISDYVAGRLDAREADLIENAMSRNRDIAAAVA
                     AARKLNSRIGRYFSQRGPQGRG"
     gene            2909..3202
                     /locus_tag="VFX46_00125"
     CDS             2909..3202
                     /locus_tag="VFX46_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599022.1"
                     /translation="MLRVFLATTNIFTSLILGALAMGVVWYLSPDLMQDFFQSASGFK
                     SWLTNLGIDPKYNNFLWFLIEERQIVFMGFVIATRVVMAMLAAIFIAPFTESI"
     gene            complement(3220..3972)
                     /locus_tag="VFX46_00130"
     CDS             complement(3220..3972)
                     /locus_tag="VFX46_00130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HEX5599023.1"
                     /translation="MKRKARSAGKRGAKELHFQGIPTAVEVRHHPAARRMTLRVSRAR
                     RAVIVTLPMQCDLEQAGQFVSRHLDWVKARLGSLPPPVPFEDGAEIPLRGVPHTIGFL
                     GYLRRGPVVSIEQKLDGRPQLVVSGESNHAPRRLLDWLIAQARKDLDERVAWHAANLK
                     LRPRGIAIRDQVSRWGSCSTTGMLSFSWRLILAPPLILDYVAAHEVAHLAEMNHGPRF
                     WALVRKTMPRMDEAKEWLRVYGMDLHRFGPEA"
     gene            complement(4102..>5239)
                     /gene="phaZ"
                     /locus_tag="VFX46_00135"
     CDS             complement(4102..>5239)
                     /gene="phaZ"
                     /locus_tag="VFX46_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX5599024.1"
                     /translation="DWQDARDVPLSAGHFDLDDYVDYMRDIFRYFGGDVHVFAVCQPS
                     VPVLIAISLMEEEDDPSIPLSMILAGGPIDTRVNPTVVNKLAEERGTDWFRHNVITSV
                     PWPRLGFGRRVYPGFLQLTGFMTMNLDRHLKAHKELFDHLVRGDGDSAEKHREFYDEY
                     LAVMDLTAEFYLQTVDTVFVRHMLPKGEMVHRGRRVDPRAIRRVALMTIEGEKDDITG
                     VGQCAAAHDLCVNIPADARVHFVCPKVGHYGIFNGSRFRVEIAPRIAQFIRAHDLRSR
                     ALDDLALMSVSPASEKAHLSPELTALAFTFAPANDSTPDPMVRKLKDQGLGRLGQTDD
                     RDNNAFDAPYRMLSLASSLFLEGLLRWPFPPGFGATKEPFEPRR"
BASE COUNT         1001 a         1563 c         1659 g         1016 t
ORIGIN      
        1 ctgttcataa acgaaagcca gcttgtgttc ctcgaggaca tgatgaaaat gcagggcttt
       61 ctcggcaact atcaaatggc tggggccttc cagcttttgc gctccaacga cctcatctgg
      121 tcgcgcatcg ttcacgacta tctgatgggc gagcggcaac ccatgttcga tctcatggcg
      181 tggaacgcgg atacaacccg catgccctac cgcatgcatt cggagtacct gcggaagctg
      241 ttcctcgaga acgatctggc cgctgggcgc tatgtggcgg acggccggcc gattgcattg
      301 acggacatcc gcgttccggt gttcgccgtg ggaacggaaa cggaccacgt cgcgccgtgg
      361 cggtctgtct acaaggtgca cctgctgctc gatacgcagg tgactttcgt gctgacgagc
      421 ggtggccaca atgccggcgt tgtgtccgag ccgggccatc cccgccgccg ctaccgggta
      481 agcaccaagg aggaggatga cctctacgtt gatgcggaca cctggttcga gcaaactccg
      541 gtcaagcagg gctcctggtg gcctgagtgg gcggcctggc tctccgaccg gtcgggcgat
      601 tggacctcgc cgccaccgat gggcgcgccc gagaagggtt acaggccgat cctcgacgcg
      661 cccgggagct acgtcttgca accgtaacga tcgaaggccg accgatgcga gcgatggtcc
      721 tcgagaagcc gggaacggag ctgaagcttc tcgacgtgcc tgtcccgaag cctggcccag
      781 gtgaggtgtt ggtacgcgtc gcggcctgcg gtgtctgccg caccgatttg catgtcgtag
      841 acggcgatct tcccgatccc aagctgccga tcatcccggg tcacgagatc gtcggctatg
      901 tcacggagct cggagagggc gttgagaaat tcaaagaggg tgaccgtgtc ggcatccctt
      961 ggcttggcag gacatgcggc cagtgccggt attgcctgag cggccgcgag aacctttgcg
     1021 agtcgcccgg cttcacgggc tataccatcg acggcggcta cgctgagttc actgtggcgc
     1081 acgccgatta ctgcttcccc cttcctatgg attacgacga cgcctcggca gcgccgctcc
     1141 tttgtgctgg gctcatcggc tatcggtcgt tgaagctcgg aggccccgga caggcgctcg
     1201 ggatttacgg cttcggcgca gcggcgcaca tcgtggcgca agtggcgaac ttcgagaggc
     1261 gacgcctctt cgcgttcacg cgacccggtg atacggcggc gcaggatttt gcgcgctccc
     1321 tgggcgccga atgggcaggc ggctcggatg aggcgccgcc ggttccgctt gatgtcgcca
     1381 tcatttttgc gcccgtcggc gagctggtgc ccgcggcgct gaaggcgatg gtgaagggcg
     1441 gcacggtggt ttgcggcggc atccacatga gcgacatccc ctcgtttccc tacgagctgc
     1501 tgtggcacga gcgcgtcgtg cgctccgtcg ctaacctcac ccgggatgat gccaccgaat
     1561 tcctcgaact tgcgccgcaa gttccggtga agaccaccat cgagacattt ccgctagagg
     1621 atgcgaacga agccttggcg cgactgcgcg aaggaaggct aacgggtgca gcggtcctcg
     1681 ttccctgaga gttttgccag ccgcggcccg ctggcatccg tggttgattg ggcgagcggc
     1741 gcgctcatgt tccgctcatg cctcgacaca cctgattgga gctttgccaa tgcttacgat
     1801 cgaggacatg atcgccttct ccggactgag ccgcgacgag gtcttggcca tagccgagca
     1861 tgagaacctg ccggaaaccg cggcgtgcgc actggcggaa tatctcatgc agcaggagca
     1921 cgggccggag cgcattcggg agatgatcat cgaagatatc cgggccgcac agcagcgggg
     1981 cgacaaggag cacgtgcaga ttctgctcca cgtgctgcat cacttcgtga gagcgcaccc
     2041 cgaatcgcta cccatctcgg gcaaacagac ggatattact tgagagaggt gacgcgccgg
     2101 gcagtagagg atgcggcgcg gcgacttcga gcggtgctcg tactgctgcc tcgataggta
     2161 cgcccgcctt ggtttagaat gcaaaatggc taagaccggc ttgcggcgag agcgccggaa
     2221 ctagcagcaa ctcacccgcc gagaagagga agaatgactg ctgccgaaac agcaatcgcg
     2281 gcgccaacga cgaattggct cgccacgact atgacaagga tctggatgag ataccgattc
     2341 gagcgcacct gaagcgtcca attctgcttt agccgcggcc ctgaggacca cgttgcgaaa
     2401 agtagcgccc gatcctggag ttgagcttgc gggctgctgc cacggctgcc gcgatatccc
     2461 gatttcggga catggcattc tcgatcaagt ccgcttccct ggcatccagc cggccggcga
     2521 cgtagtcgct aatcaggtct ggcgtgattc gatgggcaac ggggttcatc cgtgacctcc
     2581 aacttcgcgc aaacaagtgc ttcgagccaa ttcctcgaat cgttggaaaa ggtttgccaa
     2641 agaatgaacg gaggggctgt tactcgggtg acaaacccac agctttcgat cctttatagg
     2701 tttcggaaag tggtcttcga ctagaagtgt tgtccaaaaa tcctcggatc gaggcctaag
     2761 ctctgcccct tggtgatgcc accatggctg ctctccgggc acggtctatc gaagagagtc
     2821 gcggatcgaa ggtcccgagt gccgtagtag cgtacaagcc aggatctatc cggggacgga
     2881 ggcaagtcca cagaagtggg gggtaaccat gctcagagtc ttcctagcga ccaccaatat
     2941 tttcacatcg ctcatcctgg gcgctctggc catgggcgtc gtgtggtacc tctctcccga
     3001 cctgatgcag gactttttcc aatctgcgag tgggttcaag tcttggctga cgaatctcgg
     3061 aattgatccg aagtacaaca acttcctctg gttcctgatc gaggagcgtc agatcgtctt
     3121 tatggggttc gtcatcgcga cccgtgtggt catggccatg cttgcggcga tcttcatcgc
     3181 cccgttcacc gaatccattt gacctggaag gtctccgctt caggcctccg ggccgaaccg
     3241 gtgcaagtcc atgccgtaga cgcgtagcca ctccttggcc tcgtccatcc gtggcatcgt
     3301 cttgcgcacc agcgcccaga accgagggcc gtgattcatt tcggcgagat gcgccacctc
     3361 gtgtgccgcg acatagtcga ggatcaacgg cggtgcgagg ataaggcgcc acgagaacga
     3421 cagcatgccg gtcgtggagc atgagcccca acggctcacc tgatcgcgaa tggcgatgcc
     3481 gcgtggccgc agcttcaaat tggcggcgtg ccaggcgaca cgctcgtcga gatccttgcg
     3541 cgcctgcgcg atgagccaat cgagcagccg tcttggagcg tggtttgatt cgccggagac
     3601 aacgagctgt ggtcggccgt ccagcttttg ctcgatcgaa acgacggggc cgcggcgcag
     3661 atatccgaga aagccgatag tgtgcgggac acctcgcagc ggaatctcag ccccgtcttc
     3721 gaaaggaacc ggcgggggca ggctgccgag cctcgccttg acccaatcga gatgacggga
     3781 gacgaactgt ccggcctgct cgaggtcgca ttgcatcggt agagtgacga tgacggcccg
     3841 ccttgcgcga ctcactctga gcgtcatgcg gcgtgctgcg ggatggtgtc gaacttcgac
     3901 cgccgtcgga atgccttgaa aatggagctc cttcgctccg cgtttgccag ccgagcgcgc
     3961 ctttctcttc attgtttgac ctcggtagtg cctgtgttga ccgtctatca agacggggag
     4021 acacagggcc aatgagaaac gccggctaca cacaggctag agcccagtgt ggcgatcacg
     4081 cgtcactttt ttcgaaacta cctaccgacg cggttcgaaa ggttctttcg tagcgccgaa
     4141 acccggcgga aaaggccagc gcagcagccc ttccaagaag aggctgctgg cgaggctcag
     4201 catgcgataa ggcgcgtcaa acgcgttgtt gtcacggtcg tcagtctgcc cgaggcggcc
     4261 caacccttga tccttcagct tgcgaaccat gggatcgggc gtgctgtcat tggcgggcgc
     4321 gaaggtgaac gcgagagccg tcagctcagg cgaaagatgg gctttctcag acgccggact
     4381 cacgctcatg agcgcgaggt cgtcgagcgc gcggctgcgc agatcatggg cacggatgaa
     4441 ctgagcaatg cgcggcgcga tctcaacgcg gaaccgtgag ccgttgaaaa tcccgtaatg
     4501 gccgaccttc gggcaaacga agtgcactct cgcatcggct ggaatgttga cgcacaggtc
     4561 gtgagcggct gcgcattgtc cgacgcctgt gatgtcgtcc ttttcgccct cgatggtcat
     4621 cagggcgacg cggcggatcg cgcgcgggtc caccctgcgc ccgcgatgca ccatttcgcc
     4681 tttcggaagc atgtggcgga cgaagacggt gtcgacggtc tgtaggtaga actccgccgt
     4741 cagatccatc acggcgagat actcgtcgta gaactcgcga tgcttctcgg cggaatcacc
     4801 atcgccgcgc acgagatgat cgaacagctc cttgtgcgcc ttcagatgac ggtcgaggtt
     4861 catggtcatg aaccccgtga gctgcaggaa gccaggataa acccggcgtc cgaagccaag
     4921 tctcggccag gggacgcttg tgatgacgtt gtggcggaac cagtccgtgc cgcgctcctc
     4981 ggcgagcttg ttgacgactg tcgggttgac gcgcgtgtcg atcggaccgc cggcgaggat
     5041 catgctcagc ggaatggagg ggtcgtcctc ctcctccatc agcgagatcg caatcaacac
     5101 cggcacggac ggttggcaca ccgcgaacac gtgcacgtcg ccgccgaaat agcggaagat
     5161 gtcccgcatg tagtcgacgt agtcatcgag gtcgaagtgg cccgcggaca gggggacgtc
     5221 gcgcgcgtcc tgccagtcg
//
LOCUS       DASUMG010000009         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1044920, whole genome shotgun sequence.
ACCESSION   DASUMG010000009 DASUMG010000000
VERSION     DASUMG010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044920"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VFX46_00140"
     CDS             <1..239
                     /locus_tag="VFX46_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599025.1"
                     /translation="LDEPLAGMGPEESERFTILLKDLAREHAVLLVEHDMDFVFAVAD
                     WMTVMVDGAVLAEGLPKDVRADPQVQRAYLGGHA"
     gene            239..967
                     /locus_tag="VFX46_00145"
     CDS             239..967
                     /locus_tag="VFX46_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599026.1"
                     /translation="MALPSGAAPLLAGCNLHTYYGSSHILRGVSLAISTGEAVGLMGR
                     NGMGKTTLIRTLMGLVAPRSGQVLIDGQDATGMPTFRRAQAGLAYVPEERGIFASLSV
                     AENLAIAERAGIDGSSKWTRERVLELFPRLSQRLSHRGDQLSGGEQQMLAIARALLAN
                     PRLLILDEATEGLAPIIREEIWSTIAQIRNAGVATLIVDKTVDAITSLADRIVILVKG
                     EVVFEGSPASLKADPALMHRHLGV"
     gene            complement(1001..1909)
                     /locus_tag="VFX46_00150"
     CDS             complement(1001..1909)
                     /locus_tag="VFX46_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5599027.1"
                     /translation="MPKLASCPQLPGPGQIFLDHVGWMVPEMEQASLVFERLGFSLTP
                     YAIHGDRDPETGEVKPAGTANRVAILERGYLEILTPVDGVDTPVTRHMRSALAHHTGV
                     HLIAFSVSDADEAADQIRSRGFELQPTVNLKRPVEAEDGSQAEAAFTVIRPSFESFPE
                     ARAQVLRHHTPEHIWQRRYLSRDNGLTGLLEVALISADPPEAAERYSRFLGRAAEQDG
                     ASLVINLDRGGLRFLDARGAAEHFGRVSRPPMPAVGAITLTSSDLDKTRYFFLSQGLR
                     PGAINPGHLLIDESEALGVHLIIVPE"
BASE COUNT          387 a          650 c          643 g          370 t
ORIGIN      
        1 tgctcgatga gcccctggcc ggaatggggc ccgaggagag cgagcggttc accattctgc
       61 taaaggattt ggcccgcgag cacgcggtgc tcctggtcga gcacgacatg gatttcgtgt
      121 tcgccgtcgc tgactggatg acggtcatgg tcgacggcgc ggtgctcgcc gagggtctgc
      181 cgaaagatgt gcgcgccgat ccccaagtgc agcgcgccta tctgggaggg cacgcgtgat
      241 ggcgctcccc tcaggtgccg ctcctctgtt ggccggctgc aatctccaca cctactacgg
      301 ctcgagccac atccttcgcg gtgtctccct tgcgatcagc acaggcgagg ccgtgggtct
      361 gatgggccgt aatggcatgg ggaagacgac gctcatccga acactgatgg gcctcgtcgc
      421 gcctcggagc gggcaagtgc tgatcgacgg ccaggatgcg accggcatgc caacctttcg
      481 ccgggcgcag gcaggccttg catatgttcc cgaggagcgg ggcatctttg cgtccctcag
      541 cgttgccgaa aatctcgcca tcgcggaacg cgccggcatc gacggaagct cgaagtggac
      601 ccgcgagcgc gtgctcgaac tgtttccacg actgtcgcaa cggctcagtc atcgcggcga
      661 ccagctctcc ggaggcgagc agcagatgct ggccatcgca cgggcgttgc tggccaatcc
      721 gcgattgttg atccttgacg aagcaacgga agggctcgcg ccgatcattc gggaggagat
      781 ctggagcacc atcgcgcaga tccgcaacgc cggcgtcgcg acgctcatcg tcgacaagac
      841 agtcgatgca attaccagcc ttgcggaccg gatcgtcatt ctcgtgaagg gcgaggtcgt
      901 cttcgaagga tcgccggctt cgttaaaggc ggatccagct ttgatgcacc gccacctcgg
      961 cgtgtgatgg cggccgccgc ggaacggaag cccgcaactc ctactccggc acgatgatca
     1021 gatgcacgcc gagcgcttca gactcgtcga tcagcagatg tcccggattg atcgccccgg
     1081 gccgcagccc ctgactcaag aagaaatatc gcgtcttatc aaggtcgctc gaggtgagcg
     1141 tgatcgcgcc caccgcgggc atgggcggcc tcgaaactct gccgaaatgc tccgcggccc
     1201 cgcgggcatc gagaaagcgc agcccaccgc ggtcaagatt gatgacaaga gaagccccgt
     1261 cttgctcggc ggcccgtccc aagaaacggc tgtaacgctc cgctgcttcc ggtggatcag
     1321 cactgatcaa tgcaacctca agcaggccgg tcaatccgtt gtcacgcgag aggtagcgcc
     1381 gttgccagat gtgctcgggc gtatgatggc gcaacacttg agcacgcgcc tcgggaaagc
     1441 tctcgaaaga cggacgaatg acggtgaaag cagcttcagc ctgcgacccg tcctctgcct
     1501 ccaccggtcg cttgaggttc acggttggct gcagctcgaa tccgcgggat cgaatttgat
     1561 ctgccgcttc gtctgcatcg ctcactgaga acgcaatgag atgaaccccc gtgtgatgcg
     1621 caagcgccga gcgcatgtgg cgggtcactg gcgtatcgac cccatcgaca ggggtgagga
     1681 tctcgagata gccgcgttcc aggattgcaa ctcggttcgc cgtgccggcc ggcttcacct
     1741 cgcccgtttc gggatcgcga tcaccgtgga tggcgtaagg cgtcaatgaa aacccgagcc
     1801 gctcgaagac cagagaggcc tgctccattt ctggcaccat ccagccgaca tggtcgagaa
     1861 agatttgccc aggtcctggc aactgagggc acgacgcgag cttcggcacg gcttcgggtc
     1921 ccttcgagcg gcgcgatcgc aaccgcgtcc tgtcgcttgc tggatgagag cagagagttc
     1981 cctccccgtg agccgatacc gctcatcggc caacggcgca accctcctca cggtcaacgc
     2041 gagttcattt
//
LOCUS       DASUMG010000010         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_804181, whole genome shotgun sequence.
ACCESSION   DASUMG010000010 DASUMG010000000
VERSION     DASUMG010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804181"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="VFX46_00155"
     CDS             <1..577
                     /locus_tag="VFX46_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599028.1"
                     /translation="KSYLSWLIRHKAYELAYYTWLQFLSPDDLKSTGFLYNGRFERPL
                     SGLPFDWVIENGRGVSIYLTSADNPELGRSLVVEFGQGRVEFGTVSQMVLLSPGTYRF
                     TGQLKGELAGRRGLVWRVSCAEDTRAVLGTSEMMMGMAPTWRTFGFTFEVPRDRPCRA
                     QKISLGLDARSASEQLVSGMVQYADFKIERL"
     gene            complement(586..1833)
                     /locus_tag="VFX46_00160"
     CDS             complement(586..1833)
                     /locus_tag="VFX46_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HEX5599029.1"
                     /translation="MSDKSHNLRINPERLWSTIMETAAFGRTEKGGLRRLTLGPEDRQ
                     VRDWFRAACEAAGCTVKVDELGTMYAIRPGRNMSLPPIGCGSHLDTQPTGGKFDGVLG
                     TLAGLELIRVLNDAGIETEAPIAVINWTNEEGSRFAPAMMASGAYVGDFDPADILARR
                     DATGKTVAEALDEIGYRGPEKSGSVPLGAFVELHIEQGPILEAEGKTIGVVEGAQGII
                     WYDGRVVGFESHAGTTPMPLRRDALCALSEIVLAAERIAREHGPSAVATVGQATIANP
                     SRNVIPGEISFTLEFRDPQSETLDALDSKIRKAISEIAERRRVEITLEQIWRKEPVVF
                     APEIVEKIEAAAKALGLSHRRMISGAGHDACNLAKTIPTAMIFVPCKDGISHNELEDA
                     TREDCAAGANVLLHTVLSLAKVC"
     gene            2001..2318
                     /locus_tag="VFX46_00165"
     CDS             2001..2318
                     /locus_tag="VFX46_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599030.1"
                     /translation="MKIVRGLLAGVGLSACALALGAGMADAKIVCRGQFQIVDGQPIA
                     TPYCEDANVAAVAREYGIFVSASEVRRSPGTKARICRFIGHDNRVRSACAPYIPSGRQ
                     RWW"
     gene            2972..>3697
                     /locus_tag="VFX46_00170"
     CDS             2972..>3697
                     /locus_tag="VFX46_00170"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599031.1"
                     /translation="MSLFKRIFAAAAFVALALGLPFTAQAERITVTHWGVLMYGAPYA
                     VAIEKGYYKEVGLDIDGVLTSKGGGTTMRNVMAAEMPYGEVALAAVIAAMNQGIELKI
                     VHTGVRTAGEILWVTTPDSGINSVKDLAGKKVAFTSPKSVTDMLLTMVLDKHQIKAEA
                     VSAGGIGSGLTMLDQGGVVAAPVMDPIWSKVHEKYKPIFAVADELPPMVQTVGVTTAE
                     FAKNNPDKLRALTAARKKGVEFVY"
BASE COUNT          699 a         1092 c         1139 g          767 t
ORIGIN      
        1 gaaaagctat ttgagctggc tcattcggca taaagcctat gagctggcgt attacacatg
       61 gcttcaattc cttagcccag acgatctgaa aagcaccggc ttcctctata acggccgctt
      121 cgagcgccca ttgtccggcc ttccttttga ctgggttatt gagaatggca gaggcgtatc
      181 gatatatcta acctctgcgg ataaccccga gcttggacga agcttggttg tcgaattcgg
      241 gcaagggcgg gttgagttcg gcactgtctc gcaaatggtt ctattgtcac ctggaaccta
      301 tcgctttacc ggacagctca aaggcgagct ggctggtcgc cgcgggctcg tgtggcgtgt
      361 ctcctgcgcg gaagatacgc gcgccgtgct cgggacgagc gaaatgatga tgggaatggc
      421 tccgacctgg cgcacattcg gcttcacctt cgaggtgccg agggaccgcc catgccgggc
      481 gcaaaaaatc tcgctcggac tcgacgcgcg ctcggcctcg gagcagcttg tctccgggat
      541 ggttcaatac gccgatttca agatcgagcg cctataagct ttctctcagc acacctttgc
      601 cagcgaaagc accgtatgga ggagcacgtt ggcgccggcg gcacagtcct cccgcgtcgc
      661 gtcctccaat tcattgtgac tgatcccatc tttgcacggc acgaaaatca tcgccgttgg
      721 aatagtcttt gcgagattgc aggcgtcatg tcctgcgcca gagatcatcc gtcgatgtga
      781 aaggccgagc gccttcgctg cggcctcgat cttttccacg atctccggcg caaaaacgac
      841 gggctccttg cgccagattt gctcgagggt aatctccacg cggcggcgct ctgcaatctc
      901 tgaaattgct tttcggattt tggaatccaa agcatccaat gtttccgatt gtggatcgcg
      961 aaactcgagt gtgaacgaga tttccccagg gatgacgttc cgagagggat tggcaatcgt
     1021 agcttggccg accgttgcca ccgcgcttgg accatgctcg cgagcaatcc gctcggcggc
     1081 aaggacgatc tcagataatg cacaaagggc atcgcggcgc agcggcatgg gggtggtgcc
     1141 ggcgtggctt tcaaagccaa cgacccgccc atcgtaccag atgatgcctt gagctccttc
     1201 gaccacgccg atggtcttcc cctccgcctc caggatcgga ccctgctcga tatgcaactc
     1261 gacgaaagcg ccgagaggca ccgatccgga tttttccggt ccgcggtagc cgatctcgtc
     1321 aagcgcctcc gcaacggttt tcccggtcgc atcgcggcgg gccaggatat cggccggatc
     1381 gaaatcgccg acgtacgccc cagacgccat cattgccggc gcgaagcgtg agccctcctc
     1441 gttggtccag ttgatgacgg cgatcggcgc ttcagtctcg ataccggcat cgttgagcac
     1501 acgaatgagc tccaaaccgg ctaacgtgcc gagaacgccg tcgaacttgc caccggtggg
     1561 ctgcgtgtcg agatgcgatc cgcagccgat cggcggaagg ctcatgttcc ggccgggccg
     1621 gatcgcgtac atcgtcccaa gttcatcgac cttgacagtg catcctgcgg cttcgcaggc
     1681 ggcgcggaac cagtcgcgga cctggcgatc ctcgggcccc agcgtcagcc gccggagccc
     1741 acctttctcc gttcggccga atgccgctgt ttccatgatg gtcgaccaca gtcgctcagg
     1801 gttgatgcgc aggttgtgag acttgtcact catggaacat ccggctccgt gtcggcgagg
     1861 tagttttgca ggtgttcgtg ccacgggaag ctgcgaatgt ccacgggcca cctcttcgct
     1921 tcagagcaca ctggccccgg aaagtcgcca cgtggtacgt tcaaatctga ggacttgatg
     1981 gaagtcgagg tatgttcgct atgaagattg tcagagggct gcttgctggc gttggtcttt
     2041 cggcctgtgc ccttgctttg ggcgccggca tggccgatgc caagattgtc tgccggggac
     2101 aatttcagat cgtcgatggc cagccgatcg caactccgta ttgcgaggat gccaacgtgg
     2161 ccgcggtggc gcgggagtat gggatattcg tcagcgcgag tgaggtgcgg cggagccccg
     2221 ggacgaaggc gcgtatctgc cggttcatcg gccacgacaa ccgggttcgg tcagcgtgcg
     2281 ctccctatat cccttcggga aggcaacggt ggtggtgagg ggtagctttc gagctgggat
     2341 gctattcccg cttcttagct ttggtctcaa tccgtccgga gtctcaagtg caaaatccat
     2401 ttgacgggca ggttcgggtg attgtgcccg acctgccgat ttctttgctc atttttcccc
     2461 gctataccgg tttggattag ttcagctgta ccgcacgagc cttttcggct catcaacctc
     2521 gcagctctat gggcggctgc tgtagagcga gcggcaggcc tccaacgacg ccgcagcatc
     2581 agcgagcgag acgagtagct gaggctcact cctcatagat cgaccgccga gcatgagttg
     2641 tccgcctacc tggggctgtg ccctcactgg ggccggccgg cgtagcgcct ctcggcccgc
     2701 cgtgtggctg aagcccagct tcgatgccag ctcgcctctc ccagatgcgc ccatggccgg
     2761 cgaccggata tcacgcccgg gcggatggcg aaaagcgatc tcggcagcga ggccgaggca
     2821 gcatggtcac gtggatttcc actgaacagg agggtatacg atcttgccgg acgcgtatag
     2881 gggcaagcat gcacaataat ccttgtcgga aggacgtcgg catctaggcc ccggccactg
     2941 aacttcgagc cgtcaaagga ggaaacagct catgtcgctc ttcaaaagga ttttcgcggc
     3001 ggcagcattt gtcgcgttgg ctttgggcct gccattcacc gcgcaggccg agcgcatcac
     3061 ggttacgcat tggggcgtcc tgatgtacgg cgcgccctac gccgtcgcca ttgagaaggg
     3121 ctactataag gaagtgggtc tggatatcga cggcgtgctc acctcgaaag ggggcggcac
     3181 aaccatgcgc aacgtcatgg cggccgagat gccctatggc gaggtggcgc tcgcagcggt
     3241 cattgccgcc atgaaccagg gcatcgagct caaaatcgta cacacgggcg tgcgcaccgc
     3301 cggcgagatt ctgtgggtca cgacgcctga ctccggcatc aactcggtca aggacctggc
     3361 gggcaagaaa gtcgccttca cctctccgaa gtcggtgact gacatgttgc tgacgatggt
     3421 gctggacaag caccagatca aagcggaggc ggtctcggct ggtggaatcg gctctgggct
     3481 gaccatgctc gatcagggtg gtgttgtggc ggcgccggtc atggatccga tctggtccaa
     3541 ggtgcacgag aagtacaagc caattttcgc cgtggccgac gagctcccgc ccatggtgca
     3601 gacagtcggt gtgacgacgg ccgagttcgc gaagaacaat cctgacaagc tccgcgcgct
     3661 cacggccgcc aggaagaagg gggtcgagtt cgtctac
//
LOCUS       DASUMG010000011         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_402703, whole genome shotgun sequence.
ACCESSION   DASUMG010000011 DASUMG010000000
VERSION     DASUMG010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402703"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFX46_00175"
     CDS             <1..781
                     /locus_tag="VFX46_00175"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599032.1"
                     /translation="AAKAAAWVEKFGGQSAPTPAEAAADADFVFTCVGNDDDLRSVTI
                     AENGAFHGMKKGAIFIDNTTASANVARELYAAAKEKGFDFLDAPVSGGQSGAENGVLT
                     VMVGGDQPVFERAKPVIANYARMVTLIGPVGSGQLTKMVNQICIAGLVQGLAEGIHFA
                     QKAGLDIPAVMETISKGAAGSWQMENRWQTMAEGKFDFGFAVDWMRKDLMISLDEARR
                     NGASLPVVALVDQFYSEVQKMGGGRWDTSSLIARLNNADKK"
     gene            988..>2173
                     /locus_tag="VFX46_00180"
     CDS             988..>2173
                     /locus_tag="VFX46_00180"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5599033.1"
                     /translation="MALLPQGVWRKTVWRGPPGLGRLVALLTLAALMVLRVWDPPLLE
                     TLRGRIFDTYQRLHPRAVTEYPVTIVDIDERSLQEIGQWPWSRVTLASLVDRLAQRGA
                     IAIGFDILFAEPDRLSPPRIAESIPDAELKSRLAALPDSDVQLAQAISRARVVLGQGG
                     ASSLSASTTNLSELPITSVATIGDNLHRHLLRVPKLVANLPVLEKAAAGRGLFTVEPD
                     ADGVIRRIPTLLYAEGRIVPSLSIELLRVATGATTLLAKSDEAGIRSVVVAGVEIETE
                     RDGQLWLHFTPHDPRRYVSAGDVLSGRIPDDRIEGKLVLIGTSAAGLFDLRTTPLERA
                     MPGVEIHAQAIESILDNALLLRPNYALGLEISLAFVIGIGIIVVVPMLGARATVLLGI
                     ATT"
BASE COUNT          399 a          680 c          667 g          427 t
ORIGIN      
        1 cgcggccaag gccgccgcct gggtcgagaa gttcggcggc caatccgccc cgacaccggc
       61 cgaagctgcg gcggatgccg atttcgtctt cacctgcgtc ggcaatgacg acgacctgcg
      121 ctctgtgacc attgccgaga acggcgcctt ccacggcatg aagaagggcg cgatcttcat
      181 cgacaacacc acggcctccg ccaatgtcgc gcgcgagctc tacgccgcgg ccaaggaaaa
      241 aggcttcgac ttcctcgacg ctcccgtttc cggcggccag tccggcgcag agaacggtgt
      301 gttgaccgtc atggtcggtg gcgaccagcc cgtcttcgag cgcgcgaaac cggtgatcgc
      361 caattatgct cgtatggtga cgctcattgg tcccgttggc tcgggtcagc tcaccaagat
      421 ggtcaatcag atttgcatcg cggggctcgt gcaagggctc gcagaaggaa ttcacttcgc
      481 ccagaaagcc ggactcgaca tcccggccgt gatggagacg atttcgaagg gcgccgccgg
      541 ctcctggcag atggagaacc gttggcagac catggccgag ggcaagtttg atttcggctt
      601 cgccgtcgat tggatgcgca aggatctgat gatcagcctg gacgaggctc gccgcaacgg
      661 ggccagcctg cctgtggttg cgctcgtcga tcagttctac tccgaagttc agaaaatggg
      721 cggcggccgc tgggatacgt cgagcctcat cgcccggctc aacaacgctg acaaaaagta
      781 gccggaaatc tcctcctagg ggcggtgcag cgcttaggca atgtaccgcc ccttttgcgc
      841 tctgaggagc ctttccagcg agaggcctga ccctctcagc ggcccaaggc aacaccccca
      901 cctcggacaa caaatcccgc tagtgtgaat agtctcaagg ctgctaaatg gcagctcacg
      961 gagttcgtgg ggagggggcg tagggtgttg gcactcctac cgcagggagt ttggcggaaa
     1021 acagtctgga ggggtccacc cgggctgggg cgtctcgtcg ccctgctgac acttgctgcg
     1081 ctgatggtgc tccgtgtgtg ggacccgcct ctactcgaaa cgctgcgcgg gagaattttc
     1141 gatacctatc aacgccttca cccacgcgcg gtcacggagt accctgtcac gatcgtcgat
     1201 atcgatgagc gttcgttaca agaaatcggg caatggccgt ggtcgcgggt gacgctggcc
     1261 tccttggtcg accgacttgc ccaacgcggg gcgatcgcaa tcggcttcga catcctcttt
     1321 gctgaacctg accgcctgtc tcctcctcga atcgccgaaa gcataccgga tgccgagttg
     1381 aaatcgcggc tcgccgcatt gccggacagc gatgtgcagt tggcccaggc aatctcacgg
     1441 gctcgcgtcg tcctcgggca aggcggagca tcgagccttt cggccagcac cacgaacctt
     1501 tcagagttgc ccattacgtc ggtagcgacg atcggagaca atctccatcg gcacctcctt
     1561 cgggtgccga agctggtggc gaatttgccg gttctcgaaa aggcggctgc cggacggggc
     1621 ctgttcacgg tggagcccga cgcggatggt gtcattcgcc gtataccgac tctcctctat
     1681 gccgaaggcc gtatcgttcc gtcgctttcg atcgagctcc tgcgggtagc gacgggtgcc
     1741 accacgctcc tcgccaagag cgacgaggcg gggatcagaa gcgtcgtcgt tgccggcgtc
     1801 gagatcgaga cggaacgtga cggccagctt tggttacatt tcacgccaca cgacccgcgc
     1861 cgttacgtct ccgctggcga tgtgctctcg ggacggattc ctgatgaccg catcgagggt
     1921 aagcttgtcc tcatcggcac ctccgccgca ggcttgttcg acctccggac aacgccgctc
     1981 gaacgggcca tgcccggagt cgaaatccac gctcaggcga tcgaaagcat tctggacaac
     2041 gcgcttcttc tgcgaccaaa ctatgcgctg gggctcgaaa tctccctcgc tttcgttatc
     2101 gggatcggca tcatagttgt cgtgccgatg cttggcgcgc gggctacggt ccttcttgga
     2161 attgcgacta ccg
//
LOCUS       DASUMG010000012         4178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_964894, whole genome shotgun sequence.
ACCESSION   DASUMG010000012 DASUMG010000000
VERSION     DASUMG010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964894"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1088
                     /locus_tag="VFX46_00185"
     CDS             <1..1088
                     /locus_tag="VFX46_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5599034.1"
                     /translation="AQYPVLTWWGLLNVLTRAASTVTMVSIFSVGTWLASRGEVTVGE
                     IVSFVAFAGLLIGKLDQLSNFVARMFMQAPTLKSFFDLLDATVAIVERPDAKPLLNVK
                     GHVRYENVTFRYGKGEQGVFDLTFEAKPGETVALVGPTGSGKTTTLAMLQRLRDPDCG
                     RILIDGQDIRDVTLDSLRRAIAVVFQDAGLFNRSIAENIRIGRPDATDAEVEEAARLA
                     EAHGFIISKPRGYGYLAGERGAALSGGERQRIAIARAILKQAPILILDEATSALDTET
                     ENKIKRALDTLRRGRTTFIIAHRLSTVANADQILVFDKGRIVERGTFKELVRKGGLFA
                     RLVAEGGFTEPKEPDEPKLSLVAEAVS"
     gene            complement(1181..1642)
                     /locus_tag="VFX46_00190"
     CDS             complement(1181..1642)
                     /locus_tag="VFX46_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX5599035.1"
                     /translation="MVQGGGEVPRSVLFVCSMNAIRSPMAAAILRHLAGRRIYVESAG
                     VRAGERDPFAELVMSEIGIDMSRHRPRTLSDLDDTSFDLIITLSPEAHHQALELTRTM
                     AVDVEYWPTLDVSAVVELGNREQILETYRSVRDSLFARIKQRFHLEGGPSV"
     gene            complement(1644..2141)
                     /locus_tag="VFX46_00195"
     CDS             complement(1644..2141)
                     /locus_tag="VFX46_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HEX5599036.1"
                     /translation="MDASQNQPSGGTQRLISIVLDGKSIRRCNPDIEHEREVAIYDLL
                     DCNTFALEGRDDGPYKLTLSIVDDRLVFSVGNTKVDHIVTHVLSLSPLRRLIKDYFIV
                     CESYYNAIRSAPPSRIQSIDMGRRALHDEGSRILMERLKGKIRVDHDTARRLFTLVCA
                     LHWKA"
     gene            complement(2153..3451)
                     /gene="hisD"
                     /locus_tag="VFX46_00200"
     CDS             complement(2153..3451)
                     /gene="hisD"
                     /locus_tag="VFX46_00200"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX5599037.1"
                     /translation="MPLRLDTADRDFEEKFAALLAMKRESAPDVDAAVRDIIADVIAR
                     GDDALIELTRRFDRLDLTPQTLRVASEEIEGAFRTANPDVIAALHFAHDRIRAYHERQ
                     LPENLRYTDSVGAELGHRWTPVESVGLYVPGGLASYPSSVLMNAVPAKVAGVPRIAMV
                     VPSPGGELNPLVLAAAHIAGVDEIYRVGGAQAVAALAYGTATIRPVAKIVGPGNAYVA
                     AAKRQVFGIVGIDSIAGPSEVLIIADRDNDPEWIAADLLAQAEHDVAAQSILMTDDAG
                     FADAVCAAVDRQLRGLPRSSIAGESWSTFGAVIVLRSLDEAPPLADRIAAEHLEIATR
                     DPESLAERISNAGAIFLGALTPEVIGDYVAGPNHVLPTARSARFSSGLGVLDFMKRTS
                     ILRLNAAALEKLSPPAMTLASAEGLQAHRRSVEIRRSRKS"
     gene            complement(3489..3941)
                     /locus_tag="VFX46_00205"
     CDS             complement(3489..3941)
                     /locus_tag="VFX46_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HEX5599038.1"
                     /translation="MGDLKLVALDSEDLKVISAHVQDAVLQVGDMTYRPWEHRFAAIA
                     NRFDWASATGAYGGEPRLERRRTALRFDRVLEAKCQGIDPRSKGQVLELLAIKFDETE
                     PPGGYITLYFAGGPAIRLKVECIEAEMRDLGAAWKARRRPQHPDIETV"
BASE COUNT          805 a         1309 c         1304 g          760 t
ORIGIN      
        1 ccgcccaata tcccgtgctg acatggtggg ggctgctcaa cgtcctgacg cgcgccgcct
       61 ctacggtcac catggtttcg attttctcgg tgggtacgtg gctcgccagc cgtggcgagg
      121 tcactgtcgg cgaaatcgtg agcttcgtgg cgttcgccgg cctcctcatt ggaaaactgg
      181 accagctgtc gaatttcgtg gcccgcatgt tcatgcaggc tccgacactg aaatcgttct
      241 tcgacctgct cgacgcgacc gttgccatcg tggagcggcc ggatgcgaaa ccgttgctga
      301 acgtgaaggg tcacgtgcgc tatgagaacg ttactttccg ctatggcaaa ggcgagcagg
      361 gcgtttttga tctgaccttc gaggccaagc cgggcgagac ggtcgcgctc gtcggcccaa
      421 ctggctccgg caagacgacg acgctcgcga tgctgcagcg tctccgcgat cccgattgtg
      481 ggcgcatttt gatcgatggg caggatattc gggacgtcac gctggattcg ctccgccggg
      541 ccatagcggt ggtgttccaa gacgcgggcc ttttcaatcg cagcatcgcc gagaacatcc
      601 gtatcggccg cccggatgcc accgacgccg aggtcgagga ggccgcacgc ctcgccgaag
      661 cgcacggatt tatcatcagt aagccgcgag gctacggata ccttgcgggt gaaaggggag
      721 ccgcactgtc cggcggagag cgccagcgca ttgccattgc gcgcgccatt ctcaaacagg
      781 caccgatcct tatcctcgat gaggcgacca gcgcgctcga tacggaaacc gaaaacaaaa
      841 tcaaacgcgc gctcgatact ctgcgccgcg gccggacaac cttcatcatc gcgcaccgcc
      901 tttcgacagt ggccaatgcc gatcagattc tcgtttttga caaaggtcgg attgtcgagc
      961 gcggcacatt caaggagttg gtgaggaagg gcggcctgtt tgcccgactg gtcgccgagg
     1021 gcggcttcac ggagccaaag gagccggacg agccgaagct ttcgctcgtc gccgaggccg
     1081 tttcctagca gagctgccgg ctaaatagcc ggtcgttcaa gcgaggcatt caaggctgga
     1141 agatgatcgc gccgggcaga caggctttgt cccgacgttt tcagacggac ggcccacctt
     1201 cgagatgaaa acgctgcttg attcgagcga acaggctgtc gcgcacggag cggtatgttt
     1261 cgagaatttg ctcgcgattg ccgagctcga cgacggccga aacgtcgaga gtcggccagt
     1321 actcgacatc caccgccatc gttcgtgtga gctcgagggc ctggtgatga gcttccggcg
     1381 aaagggtgat gatgagatcg aagctcgtat cgtccaggtc gctcagggtc cgcggccgat
     1441 ggcgagacat gtcgatgccg atttcactca ttaccaattc agcgaacggg tcgcgctccc
     1501 ctgcgcgcac gcctgccgac tcaacgtaaa tgcgacggcc agccagatgc cgaaggatcg
     1561 ccgccgccat cggtgaccgg attgcattca tagagcagac gaacaacaca gatctcggta
     1621 cctccccgcc cccttggacc atctcaggcc ttccagtgca gcgcacatac gagcgtgaac
     1681 agccgccgcg cggtgtcgtg atccacccgg atcttgccct tgagccgctc catgagaatc
     1741 cggctgcctt cgtcgtgcag agcgcgtcgg cccatgtcga tcgactgaat gcgtgagggc
     1801 ggcgcagagc ggatcgcgtt gtaatagctc tcgcagacga tgaagtagtc tttgatcagc
     1861 cgccgtaacg gcgacagcga cagcacgtgg gtcacgatgt ggtcgacctt cgtgttgccg
     1921 acgctgaata ccagccggtc atcgacgatg ctgagcgtca gcttgtacgg gccgtcatcg
     1981 cgcccttcga gcgcgaacgt attgcaatcg agaagatcat agatcgcgac ctcgcgttcg
     2041 tgctcaatgt ccggattgca gcgccgaatg gatttcccat cgagaacgat ggaaatgagc
     2101 cgctgcgtac ccccgcttgg ctggttttgg gatgcgtcca tgccggaaat cttcagcttt
     2161 ttcttgagcg ccggatttcg accgagcgcc tgtgcgcttg gagcccctca gcagacgcga
     2221 gagtcatggc cgggggcgac agcttctcga gtgcagctgc attgaggcga agaattgagg
     2281 ttcttttcat gaaatcgagc acgccgagcc cagaggagaa ccgggcgctg cgggccgtcg
     2341 gcaggacgtg gttcgggcct gcgacgtagt caccgatcac ctcgggtgtc agagcgccga
     2401 ggaaaatggc gccggcattg gaaatgcgct cggcgagaga ctcaggatcc cgagtagcga
     2461 tttccaagtg ctcggctgca atccgatcgg cgagcggcgg cgcctcgtcc agcgaacgaa
     2521 gcacgatcac cgcaccgaag gtgctccagc tctcgccagc gatgctgctg cgcggcagcc
     2581 cgcgaagctg ccgatcgacg gcggcacaca ccgcgtccgc gaacccggca tcatccgtca
     2641 tcaggatgga ctgggccgca acgtcgtgct ctgcctgggc gaggagatcc gccgcgatcc
     2701 actccggatc gttgtcgcga tcggcgatga tcaggacctc ggagggcccc gcgatcgagt
     2761 cgatgcccac gatgccgaaa acctggcgct tcgctgccgc cacgtaggca ttcccgggac
     2821 cgacgatttt cgcgacgggc cggatcgtcg ccgtgccata ggcgagcgcg gcaacggcct
     2881 gcgcccctcc aacgcggtag atctcatcaa caccggcaat gtgggccgct gccaggacaa
     2941 gtgggttcag ctctccgccg ggtgagggaa ctaccatcgc aatccttggc acgccggcga
     3001 ccttggcggg cacggcattc atcagcacgg agctgggata ggaggcgagg ccgccaggca
     3061 cgtacagccc caccgattcg accggcgtcc aacggtgacc gagctccgca ccaacggagt
     3121 ccgtgtagcg caaattttcg ggaagctgac gctcgtgata ggcgcggatt cgatcgtggg
     3181 caaagtgaag ggccgcaatc acatcgggat tggcggtgcg aaaggcccct tcgatctcct
     3241 ctgatgcaac gcgcaaggtc tggggggtga ggtcgagccg atcgaaccgg cgtgtcagct
     3301 cgatgagcgc gtcatcgccc cgcgcgatga cgtccgcgat gatgtcccgg acggcagcgt
     3361 caacgtccgg cgcgctctcg cgcttcatgg cgagcagcgc ggcgaacttt tcctcgaagt
     3421 cccgatcggc ggtatccagc ctcaggggca ttcggcttcg aaccgcccaa tcggcggcct
     3481 cctaggattc agactgtctc aatgtcggga tgctgtggcc ggcggcgcgc cttccaggcc
     3541 gcgcccaggt cccgcatctc ggcctcgatg cactccacct tcagccgtat cgccggacca
     3601 ccagcaaagt acagcgtaat gtaaccaccc ggcggctctg tttcgtcgaa cttaatggcg
     3661 aggagctcaa gaacctgccc cttcgacctc ggatcgatcc cctggcactt tgcctcgagc
     3721 acccgatcga agcgcagggc cgtgcgacgg cgctctaacc gcggctcgcc gccgtaagct
     3781 cccgtggcac ttgcccaatc gaaccgattg gcgatagcgg cgaaacgatg ctcccatggc
     3841 cggtaggtca tatccccgac ttgcagcacc gcatcctgaa catgggccga gatgaccttg
     3901 aggtcctcgg aatcgagtgc caccagcttg agatcaccca tatcttccgt tcctgcacct
     3961 ggcacgggcc acacacacgc gaaacccaca caggcgagag atagcggcgg tgctgcgaag
     4021 gtgcaacggg tgggacgatt atgccaacct cgaacggcgc tcagccgctg atgcgctcga
     4081 tgatcgcccc gcaagcagaa agtttgtgct ccaggcgctc gaagccgcgg tcgagatggt
     4141 aaacgcggtt gacgaccgtc tcgccgtcgg cgatcagc
//
LOCUS       DASUMG010000013         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_322986, whole genome shotgun sequence.
ACCESSION   DASUMG010000013 DASUMG010000000
VERSION     DASUMG010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322986"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1224
                     /gene="fdhD"
                     /locus_tag="VFX46_00210"
     CDS             343..1224
                     /gene="fdhD"
                     /locus_tag="VFX46_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX5599039.1"
                     /translation="MKEFEVRPTPDDPRLSVTVSGIDHEGKPVETYVVTERPLTLFLN
                     SREIVTMMTIGDRPDLLAVGYLLNQNMLRPDDEITAIEYHEDIETVVVRTRRQTDFEE
                     KLKKKIQTSGCAQGTVFGDLMEEFDQIKLNPDARFRTSWLYQLMKKINTAPSLYLKAG
                     AIHGCVLCQEDRPLIYMEDVGRHNAVDKIAGYMFLNNIKPDDKIFYTTGRLTSEMVIK
                     TVKMRIPILISRSGFTAWGVDLARRAGLTLIGRARGKRFIALSGLERIIYDADLARVE
                     EEDRKFVRKGSLATAED"
     gene            1231..1872
                     /gene="mobA"
                     /locus_tag="VFX46_00215"
     CDS             1231..1872
                     /gene="mobA"
                     /locus_tag="VFX46_00215"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HEX5599040.1"
                     /translation="MVDRAKNRVTGVLLAGGLSRRMGGGDKCLRDLAGKPMLAHIIER
                     LQPQVGAMVINANGDPARFAQFGLDVVPDTIEGFAGPLAGVLAGMRWSATHAPEACWI
                     VTVSTDAPFLPLDLTERLLASVPDERTIALAASGGDVHPVIGLWPVALADDLDEQLKA
                     GVRKVLHWTDRHFTVVVEFPFLQLHGRIIDPFFNANTPEELEEARALMESRSP"
     gene            1869..2459
                     /gene="mobB"
                     /locus_tag="VFX46_00220"
     CDS             1869..2459
                     /gene="mobB"
                     /locus_tag="VFX46_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEX5599041.1"
                     /translation="MRSAPRASDTPIIGIAGWKNSGKTMLVVRLVEEFTRRGLKVATV
                     KHAHHAFQVDEGETDSARHRRAGAQQVAVVSSRRWAMIRELGEEPEPSFEEVISWLDP
                     CDLIIVEGYKFSPIPKIETRRLESPTKRPLAVEDPMVIAIASDHPVEGTALPVFSLDD
                     IQAIADFIDKSIGPLGKLREAEAGTAPSAPAQNRSK"
BASE COUNT          488 a          762 c          791 g          493 t
ORIGIN      
        1 gataccagcc cgagatcgac gatattgccc tccaggtcag gtcccttcac gcgccgcagc
       61 tcctccagga cctgcgtctt cgtcactgac atcgattttc tccaaggcgt cttttgcggc
      121 cagcgaattg gcctgctgaa attttggaat cattgtgaca ggtgacggcc tcgaagtcac
      181 ggcttgcaac ctgcgctaca gagccacgaa atgctagaga gcgcacgctc gcgttgccgt
      241 gttccggtgc ccgattgcca gcaccggtat cgtggggcct aatcacccgg aaataccccg
      301 gtgcggcccg agcgccgcgg cccgaagtca ggagcgagga atatgaagga attcgaggtt
      361 cggccgacgc cggatgatcc gcgcctttcg gtaaccgtct cggggatcga tcacgagggc
      421 aagccggtcg agacttacgt cgtcacggag cggccgctga cacttttcct caattctcgc
      481 gaaatcgtga ccatgatgac gatcggcgat cggcccgacc tgctcgccgt cggttatctg
      541 ctcaatcaga acatgctgcg tcccgacgac gagatcacgg cgatcgaata ccacgaggac
      601 atagagacgg tggtggtgcg gacccgccgc cagaccgact tcgaggaaaa gctcaagaag
      661 aagatccaga cctccggttg cgctcagggc acggtcttcg gcgacctgat ggaggagttt
      721 gaccaaatca agctcaatcc ggacgcccgc ttcagaacga gctggctcta tcagcttatg
      781 aagaagatca acaccgcgcc gagcctctat ctcaaggccg gcgccattca cggctgcgtg
      841 ctttgccagg aggaccggcc gctcatctac atggaggacg ttggccggca taacgctgtc
      901 gacaagatcg ccggctacat gttcctcaac aacatcaagc cggacgacaa gatcttttac
      961 accaccggcc ggctgacctc agaaatggtc atcaagaccg tcaagatgcg tatcccgata
     1021 ttgatatcgc gctctggctt taccgcctgg ggcgtcgacc tcgcgcgccg cgctggcttg
     1081 acgctgattg gccgggcgcg ggggaagcga ttcattgccc tatccggcct cgagcgcatc
     1141 atctacgatg ccgacttggc gcgtgtggag gaggaagatc gcaagttcgt gcgcaaaggc
     1201 agcctcgcca cggccgagga ctgatcgtga atggttgatc gtgcgaaaaa tcgggtgacg
     1261 ggcgtcttgc ttgcgggcgg cctctcgcgg cggatgggcg ggggcgataa atgcctgcgt
     1321 gacctcgcgg gcaagccgat gctggcgcac atcatcgagc ggcttcagcc ccaggtcggc
     1381 gctatggtca tcaacgccaa tggcgatcct gcccggttcg ctcagttcgg cttggatgtc
     1441 gtgcccgata caattgaggg tttcgccggc ccgctcgcag gggtgctggc gggaatgcgg
     1501 tggtcggcca cccatgcgcc ggaggcctgc tggatcgtca cagtctccac agacgcacca
     1561 tttctaccgc tcgacctgac agagcggtta ctggcgtcgg ttcccgacga gcggactatc
     1621 gcgctcgcgg cttctggcgg cgatgtgcac ccggttattg gcttgtggcc tgtggcattg
     1681 gccgacgacc tggatgagca gctcaaggcg ggcgtgcgca aggtcttgca ctggacggat
     1741 cgccatttca ccgttgtggt cgagttcccg tttctgcagc tccatggccg cataatcgac
     1801 ccgttcttca atgcgaacac gcccgaggag ctggaagaag ctcgcgccct gatggagagc
     1861 agaagcccgt gagatcggca cctcgcgcat ctgatacgcc catcatcggc atcgcgggct
     1921 ggaagaactc cggcaagacg atgctagtgg ttcgtcttgt cgaggagttc acgcggcgcg
     1981 gtctcaaggt ggcgaccgtg aagcacgccc accatgcttt tcaggtggac gagggtgaaa
     2041 cggatagcgc ccgccatcgc cgtgcggggg cgcagcaggt cgccgtcgtc tccagtcggc
     2101 gttgggcaat gatccgcgag cttggcgagg agccagagcc cagcttcgag gaggtgatta
     2161 gctggctgga cccgtgcgat ctgatcatcg tcgagggcta taagttttcg ccgatcccca
     2221 agatcgaaac gcgccggctg gaatcaccaa cgaagcgacc tctggcggtg gaggatccga
     2281 tggtcatcgc catcgcgtcc gatcatccgg tcgaaggcac cgcgctgccc gtattctcgc
     2341 tcgatgacat ccaggccatc gcggatttca tcgacaagtc gatcgggccg ctcgggaagc
     2401 tgcgagaggc cgaggccggt actgcgcctt cggctccggc tcagaatcga agcaaatgaa
     2461 gaagattatc gctcttgcgt gggcaacgtc gtttcttgcc ctcttcttgc cgatagcggt
     2521 tacggctggt ggca
//
LOCUS       DASUMG010000014         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_162582, whole genome shotgun sequence.
ACCESSION   DASUMG010000014 DASUMG010000000
VERSION     DASUMG010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162582"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VFX46_00225"
     CDS             complement(<1..486)
                     /locus_tag="VFX46_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5599042.1"
                     /translation="MPDLEIQLEVNGRKVRRFAKPYQRLIDFLRDDLDLTGTKEGCGA
                     GECGTCSVFVNGKLVKSCLMPVAKANGAKIETIEGLAPPGELTTIQRAFYKTGASQCG
                     YCIPGMVMAATAALRENPNADVEEIKERLGGNICRCTGYAKIIEAVEIARDVIAGRIG
                     PA"
     gene            complement(479..1396)
                     /locus_tag="VFX46_00230"
     CDS             complement(479..1396)
                     /locus_tag="VFX46_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX5599043.1"
                     /translation="MLLCDEYLTVTTLGEALAELQAHRGRCKIVAGATDILPWAREGR
                     AGDVHVPVLIDISRIPELSGYQVSNGRARLGANVVFQRFLTDDLLREHLPCMPYCAVW
                     FADDQIREQATLAGNIVNASPAADGTPPLLAMDAEIEIARLVQGSIEHRSLPLSEFVL
                     GPGKTALEPDEIVTAITCTSMAGYGGSFQKVGRRRSLVISTVCAAALAKIDSSGQVIE
                     DVRVAVGGIGPVPMRLKDVEDKLKGERISQDLIARVSALAADRVASRTRQEYRRSVVC
                     GFVQAAIEEALVACGAAAPGSAQKEAAHA"
     gene            complement(1413..1898)
                     /locus_tag="VFX46_00235"
     CDS             complement(1413..1898)
                     /locus_tag="VFX46_00235"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5599044.1"
                     /translation="MNMVKRLGIASALMLAVALPASAQDLPLSPNGVKTLAPEGAIKP
                     TGTWNLGTRAGDFVFVAGMRGIDPKENKLVEGDEARIRQAFLNMKHIAESEGATLRDA
                     VRIVVYVTDMYRYRPIVNKVQQELWGDGPYPPRTIVEVDRLNQDDIFEVEGTFYAPLK
                     K"
     gene            complement(1922..>2652)
                     /locus_tag="VFX46_00240"
     CDS             complement(1922..>2652)
                     /locus_tag="VFX46_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5599045.1"
                     /translation="FTTDGGFCEYQVNNINTLIRISDDMSDEEATLVVTAGTAMYGLT
                     ELGGLVAGESVVVTGPGPIGLLGVAVAKALGAQPVILTGTRDNRLQIGKELGADHVIN
                     IRKVDDVVQAVKDLNGGTGVDYVLECSAAPNALNEAIKMVNRGGKVCLAAFPHGEVPV
                     DVAHIVRNNIYVYGIRGEGKSATHRAEAFMRQKRFDATKIHTHTFPLSDLPTAIRYAK
                     DRVEDAIKVVVKANMDKVQKVAAE"
BASE COUNT          540 a          832 c          732 g          548 t
ORIGIN      
        1 agcagggccg atgcggcccg caattacatc tcgcgcaatt tcaacggctt caatgatttt
       61 cgcataaccc gtgcagcggc agatattgcc gccaaggcgc tccttgatct cctcgacatc
      121 agcattggga ttttcgcgca atgccgccgt ggcagccatt accatgccag gaatacagta
      181 gccgcactgg ctggcgccgg tcttgtaaaa tgcccgctga atggtggtga gctcacccgg
      241 aggcgcgagc ccctcgattg tttcgatttt cgcgccattt gccttcgcca cgggcatcag
      301 gcacgatttc acgagcttgc cattgacgaa aacggagcag gtgccgcatt cgccggcacc
      361 acaaccttcc ttcgttccgg tcaggtcgag gtcgtctcgc aaaaagtcga tcagccgctg
      421 ataaggcttg gcgaaccgac gcaccttgcg tccgttcact tccaattgaa tctcgagatc
      481 aggcatgcgc tgcctccttt tgagcggagc cgggtgcagc tgctccgcag gcgacgagag
      541 cttcctcgat agccgcttgc acgaatccgc agacaacgga gcgccgatac tcctgacgtg
      601 tgcgcgatgc gacccggtca gccgccagcg cgcttacacg cgcgatgaga tcctgcgata
      661 tgcgctcgcc tttcagcttg tcttccacgt ccttcaggcg catcgggacg gggccaattc
      721 caccgacggc aacacgaacg tcctcaatca cctgaccaga cgaatcgatc ttggccaatg
      781 ccgccgcgca aaccgtcgag atgacgagcg aacggcggcg accaactttc tggaatgaac
      841 cgccataacc agccatcgat gtgcaggtta tggcagtgac aatttcgtcc ggctcaagcg
      901 ccgttttccc aggcccgaga acgaactcgg acagcggcag ggaccggtgc tcgatgctcc
      961 cctgcacaag ccgggcaatc tcgatttcag catccatggc aaggagcggc ggcgttccgt
     1021 ccgcagccgg agatgcattt acgatattcc cggccagcgt cgcttgctcc ctaatctgat
     1081 catctgcaaa ccaaacggcg cagtacggca tgcagggcaa atgctcccgc agcagatcgt
     1141 ccgtcaggaa ccgctggaac acgacgttgg cgcccaaacg ggcacgccca ttggaaacct
     1201 gatagccaga cagctcggga atgcggctga tgtcgatcag aactggcacg tgaacgtcac
     1261 ccgcccggcc ctcgcgagcc caaggcaaaa tgtccgttgc accagccacg atcttgcacc
     1321 gcccacgatg ggcttggagc tcggcgagcg cctcgccgag cgtcgtgact gtcaggtatt
     1381 catcgcagag gagcatttct ctcccgaatg acctatttct tcaaaggtgc gtagaacgta
     1441 ccctcgacct cgaaaatgtc gtcttgatta agccgatcga cctctacgat agtccgaggc
     1501 ggatacgggc cgtcacccca cagctcctgc tgcaccttat tcacgatcgg tcgataccgg
     1561 tacatgtcgg tgacgtagac aacgatcctc acagcgtcgc gcagggtggc accctcggat
     1621 tcggcgatgt gcttcatgtt gagaaacgct tggcggatcc gtgcttcatc gccctcaacg
     1681 agcttgttct ctttcggatc aattccgcgc atgccggcga cgaaaacgaa atcgccggcg
     1741 cgggtaccga gattccaggt tccagtcggt ttaatggctc cttcgggcgc cagcgtcttg
     1801 acgccgtttg gtgagagcgg gaggtcttgc gcagacgctg gcaacgccac tgccagcatc
     1861 agagctgacg caatacccaa ccgtttcacc atgttcatgg ctattcctcc tcgatggcag
     1921 gttattccgc agccactttt tgaaccttat ccatgttggc cttcacgacc accttgatcg
     1981 catcttccac gcgatccttc gcatagcgga ttgctgttgg cagatccgat agcgggaagg
     2041 tgtgcgtgtg gatcttcgtg gcatcaaaac gcttctgcct cataaaggct tcggcccggt
     2101 gggttgccga tttcccttcc ccgcggatgc cgtagacata aatgttgttg cggacgatgt
     2161 gcgcgacgtc gacgggtacc tccccgtgcg ggaaggcggc gagacagacc ttgccgcccc
     2221 gattaaccat cttgatcgct tcgttcaaag cattcggagc ggcggagcat tcgagaacgt
     2281 agtcgacacc cgtgccgccg ttcaaatctt tcacggcctg aaccacgtca tccaccttcc
     2341 ggatattgat gacgtgatca gcgccaagct cctttccgat ttgcaggcgg ttgtcgcgcg
     2401 tgccagtcag aataaccggc tgcgcgccca gggctttggc aacggcgaca ccgaggagcc
     2461 cgattggacc gggaccggtt accacaacgc tttcgcccgc aacgaggccg ccaagctccg
     2521 tcaggccgta catggcggtt ccggccgtaa cgaccagagt tgcctcctcg tccgacatat
     2581 cgtcggagat gcggatcagc gtattgatgt tgttgacctg atactcgcag aagccaccat
     2641 ccgtggtgaa ac
//
LOCUS       DASUMG010000015         2049 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_483855, whole genome shotgun sequence.
ACCESSION   DASUMG010000015 DASUMG010000000
VERSION     DASUMG010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483855"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1626
                     /locus_tag="VFX46_00245"
     CDS             103..1626
                     /locus_tag="VFX46_00245"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5599046.1"
                     /translation="MSTRKLSLLAGVVVAALVAAYWAPRLQAELSPTRTISLYNIHTK
                     ETITVEYKKDGKYVPAAMEQINWIMRDWRKNEATRMDPELIDLLWEMHAELGSKEPIH
                     IISGYRSRSTNEMLRKTRGGQASQSRHIMGKAADVHFPDVPIRRLRYSALVRERGGVG
                     YYPTSAIPFVHVDTDRVRAWPRLPRHELALLFPDGRTKHIPADGKPLTPADVQIARTK
                     HVDLAQQIAAYHDLRRGAGRRTQIASASSKAPPQTGPKSVQQQRIASATPSRPEPSPT
                     TATMEPPAPKLLVGPQPASRPAVASRPSDEDRMKLTQLASLASLGPEPPRLVQASFGP
                     NEPPSSEQDLTNSLPVTRKSEDMQLAALDSQLDAEPRSRLGRFGWGAAWRPDTEPEIE
                     APPTEWAVAPEYDDEHPEELFYRPFPIIPYLTETPSPDDPALARMEHPDVAKTFELLD
                     QAGELPPMRLRPEPQVVELLWAQQFRGEAVPLARVFADREPSPDGLLTERPVRISSR"
     gene            1748..>2049
                     /locus_tag="VFX46_00250"
     CDS             1748..>2049
                     /locus_tag="VFX46_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX5599047.1"
                     /translation="MTTSALSHKPVSALTPEEAQAELARLAAEIAHHDRLYYQLAAPE
                     ISDAEYDALVRRNAEIEARFPELIRPDSPSRRIGAPPVEAFGKVRHRVPMLSLNNA"
BASE COUNT          407 a          689 c          604 g          349 t
ORIGIN      
        1 acaatccagc gttagcgcta aagaactgct acgggccgct ccaaatcaaa gcacactgcg
       61 gcccttaagg cagacagcgt tcggtcaggg gtggggctag ttttgtcaac gcgtaagctg
      121 agtctcctcg ccggtgtcgt cgtggcggca ctcgttgctg cttattgggc gccccggctc
      181 caggcggaat tatcgccgac ccgcacgatc tccctataca acatccacac caaggaaacc
      241 ataacggtcg agtacaagaa ggacggcaag tacgtccccg cggcgatgga gcagatcaac
      301 tggatcatgc gggattggcg caagaacgaa gcgacccgca tggaccccga gctgatcgac
      361 ctgctctggg aaatgcacgc ggagctcggc tccaaggagc ccatccacat catctcaggc
      421 taccgctcgc gctccacgaa cgaaatgttg cgcaagacgc gtggtgggca ggcatcgcag
      481 agccggcata tcatgggcaa ggcagccgat gtccatttcc ctgatgtgcc gatccgccgt
      541 cttcggtatt ccgcattggt gcgggagcgc ggcggtgtcg gctactatcc aacatcggca
      601 attccgttcg tgcacgtcga caccgaccgc gtgcgggcct ggccgcgcct gccacgccac
      661 gagctggcgc tgctattccc ggacgggcgg acgaaacaca ttccggcaga tggcaagccg
      721 ctgacacccg ccgacgtgca gatcgccagg acaaagcacg tcgatctcgc ccagcagatc
      781 gctgcatatc acgacctgag acgaggagct ggccggcgga cgcaaattgc cagcgccagt
      841 tcaaaggctc ccccacagac cgggccaaaa tccgttcagc agcagcgcat cgcttcggcc
      901 acaccgtcca ggccagaacc cagtccgacc acggccacca tggagccgcc cgcgccaaaa
      961 cttctcgtcg gcccccaacc tgcaagccgt cctgccgttg cgagcaggcc gtcggatgag
     1021 gatcgtatga aactgacgca actcgcgtcg ctcgcaagcc ttggcccgga gccacctcgc
     1081 ctcgtccagg catccttcgg acccaacgag ccgccttcct cagagcagga tctcacgaac
     1141 tcgctgccag tcacgcgaaa atcggaagat atgcagttag cggccctcga ttcgcagcta
     1201 gatgcggagc cgagatctcg gctcgggcga tttggatggg gtgcggcgtg gcgaccggat
     1261 acggagcctg aaatcgaggc acctcccaca gaatgggccg tagcacccga atacgacgat
     1321 gagcaccccg aggagctctt ctaccgtccg tttccgatta tcccttattt gaccgaaaca
     1381 ccatctccgg acgatcccgc tctcgcgcgc atggagcacc cggacgttgc caagaccttt
     1441 gagcttttgg accaagctgg cgagcttccg cccatgcggt tacggcccga gccgcaggtc
     1501 gtcgagctgc tgtgggccca gcagttccgc ggcgaagccg tgccgctggc gcgcgtgttc
     1561 gccgatcgag agccgtcgcc tgacgggctg ctgaccgagc gtcctgtgcg catcagctcc
     1621 cgctgagtcc gagcgaagct gatgctgtca gcggcgtaac ccggcccggg gtagaaccag
     1681 gattggagct tgattcagtt ggggcagcgt gccaccaagc gctgactcat gctgctgtgg
     1741 ctgccacatg acaacctcgg ctctctctca taagcctgtc tcggcactca ctccggagga
     1801 ggcgcaggcg gaactcgccc gactcgcggc cgaaattgct catcacgacc gcctctacta
     1861 tcagctcgcg gcgcctgaaa tctccgacgc cgagtatgac gccctggtcc gccgtaacgc
     1921 ggagatcgag gcacgctttc ccgagctaat ccgccctgac agcccgtcac gtaggattgg
     1981 cgcgccgccg gtcgaggcgt tcggcaaggt tcggcaccgc gtgcccatgc tgtcgctcaa
     2041 caacgcctt
//
LOCUS       DASUMG010000016         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_323427, whole genome shotgun sequence.
ACCESSION   DASUMG010000016 DASUMG010000000
VERSION     DASUMG010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323427"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..445
                     /locus_tag="VFX46_00255"
     CDS             65..445
                     /locus_tag="VFX46_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599048.1"
                     /translation="MTKPIPDKAEVALEYPEKLYIGTFERTARFDAHLDETGISLSLH
                     RLGDADVRKSVRIHLHYALFAEILQDLARTVSAVPPADAAHRDALRDAAKALYRALDP
                     SRSDKDDLAQMTPDDEVLLLHVME"
     gene            complement(530..853)
                     /locus_tag="VFX46_00260"
     CDS             complement(530..853)
                     /locus_tag="VFX46_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5599049.1"
                     /translation="MRGTPVRLMAMVVLTLATGPGEAQEIGQPGRGLVAAQRLCAQCH
                     AVQKGQTRSPNGNAPRFQTIASVPGMTAIALSAALNTSHRSMPNIMLERDEQADIIAY
                     ILSLK"
     gene            986..1681
                     /locus_tag="VFX46_00265"
     CDS             986..1681
                     /locus_tag="VFX46_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5599050.1"
                     /translation="MRAADVMITNVIAVGPDACVQDVARILLDSRISAVPVIAADGKL
                     LGIVSEGDLMRRAEAGTGRRRPWWLAILTGREVLANEYIREHSRRVTDVMTRNVVTAT
                     ADTPLSTIANLLEKNAIKRVPIVEEGKVIGIVSRANLLQALASPGTRAKGAATGADDS
                     KIRETVLSQLKAEPWTRPSLINVIVQDGAVELWGIVDSASEKKAVRVAAEVTPGVRAV
                     NDNLIIRPAESGS"
     gene            1752..>2005
                     /locus_tag="VFX46_00270"
     CDS             1752..>2005
                     /locus_tag="VFX46_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5599051.1"
                     /translation="MAEAATKLPVKNEESKTERPTEWRPFESLRREVEHLFDDFQLGP
                     WRSPFGRTLFEPFRRGEMGWKTPAVDVVEKEKAYEITAELP"
BASE COUNT          418 a          588 c          620 g          379 t
ORIGIN      
        1 atcattttcg ggccgtcgga tcacctctat caggctgcga gggttaattg ctgaggcagg
       61 tgccatgacc aagccgatcc ccgacaaagc ggaggtagca ctcgagtatc cggaaaagct
      121 ttacatcggg acattcgaga gaaccgcgcg cttcgatgcc catctcgacg aaaccgggat
      181 ttcgctgagc ctgcaccgtc tgggtgacgc ggacgtgcgc aagtccgtcc gcatccacct
      241 tcactacgct ttgtttgcag agattctcca ggacttggct agaacggtgt ctgcggtacc
      301 gccggccgac gccgcgcacc gcgatgcgtt gcgagatgcg gcaaaggctc tctaccgtgc
      361 cctcgacccc agccgaagcg acaaagacga cctcgcgcag atgacgccgg acgacgaggt
      421 tctgttgctg catgtcatgg aatagtggcc gcctccgcat gatgccgcca cgttcggaat
      481 ctgcaggggc aacgtcgtac gcgcttcgcc atcaactcct gtcatcggct catttgaggc
      541 tgaggatgta ggcgatgata tcggcctgct cgtcgcgctc cagcatgatg ttaggcatcg
      601 agcgatgtga ggtgttgagc gcggccgaca gcgcaatcgc agtcatcccc ggcactgacg
      661 caatggtttg gaagcgaggc gcattcccgt tgggagatcg tgtctgccct ttttggacgg
      721 catgacattg cgcacagagc cgctgtgccg cgacgaggcc gcggccgggt tgaccgatct
      781 cctgggcttc ccccggtccg gtagccagcg ttaacacaac cattgccatg agccgaaccg
      841 gcgtcccgcg cattttcgca tctccaaaat tcatcttcgt gcctgcatca tatcactacg
      901 taagacttta acggtcttga tgcaaatcaa agataacgcc tgtccaagcg gttaggcttt
      961 ctaatatcag aaagggagcc gtgccatgag agctgccgat gtgatgatca cgaatgtcat
     1021 cgcggtcggc cctgacgcat gcgttcagga cgttgcccgc attttactcg attcacgcat
     1081 cagtgccgtt ccggtgattg cagccgacgg caagctgttg ggaatagtca gcgaggggga
     1141 cctgatgcgt cgcgccgagg ccggcactgg ccgcaggcgc ccctggtggc ttgccattct
     1201 caccggcaga gaggtgctcg cgaatgagta catcagggag cattcgcggc gggtcaccga
     1261 tgtcatgacc cgcaacgtgg tgacagcaac tgccgatacg ccgctttcga ccattgcgaa
     1321 tctgctggag aaaaatgcga tcaagcgggt cccgatcgtc gaggaaggca aggtcatcgg
     1381 catcgtcagc cgcgcgaacc tgctgcaagc attagcgagc ccgggcacgc gtgcaaaggg
     1441 tgctgccacc ggcgccgatg actcgaagat ccgcgagacg gtgctcagcc aactcaaggc
     1501 tgagccttgg accaggccgt cgctgatcaa cgtgatcgtg caggatggcg cggtggaact
     1561 gtggggcatc gtcgattccg cctctgagaa gaaggcggtt cgggtggccg cagaggtcac
     1621 gcccggcgtg cgcgccgtca acgacaatct catcattcgc ccggcggaat ccggttcctg
     1681 accggcggca ggcgacatgg caatggattt tgcgccctta acgacgtcac gagcaaagaa
     1741 ggagaactaa aatggctgaa gctgcaacca agctgccggt gaaaaatgag gaaagcaaga
     1801 ccgagcgacc aaccgagtgg cggccgttcg aaagcctgcg ccgggaggtc gaacatctgt
     1861 tcgacgactt ccaattgggc ccgtggcggt cgccattcgg ccgcacacta ttcgagccgt
     1921 tccgacgcgg cgagatgggc tggaagactc ccgccgtcga cgttgtcgaa aaggagaagg
     1981 cgtacgagat cacggcggag ctgcc
//
LOCUS       DASUMG010000017         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_564541, whole genome shotgun sequence.
ACCESSION   DASUMG010000017 DASUMG010000000
VERSION     DASUMG010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564541"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VFX46_00275"
     CDS             complement(<1..788)
                     /locus_tag="VFX46_00275"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599052.1"
                     /translation="MSFTQLVDVWLRRRWRPDPDALSQWAELQPAVVVTGGSEGIGFE
                     IARRFAKRGRAVVLVARRSEPLETAASAIRQEFGTTVLTVPLDVTRPDAPQALQERLL
                     QNGFYADVLVNNAGMGLSGDYISQEPEQLGQLIELNVRALSVLMRHFLPAMCVRGRGG
                     VLNVASLGGYAPGPYQAAYYASKSYVIALTEAVAWETRGLGVRVAVFAPGPVRTRFHA
                     RMGAESALYRWLIPAPSPSLAAGSAVRWFEWGRTVITPGVMGPLL"
     gene            complement(785..1762)
                     /locus_tag="VFX46_00280"
     CDS             complement(785..1762)
                     /locus_tag="VFX46_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5599053.1"
                     /translation="MPMTRDIDIALLRAFLAVVETGGVTAAARILNRTQAAVSLQIKR
                     LEDLFGTELFVRERKRFTLSPAGERLFGNAQRLVALNDATWGAMTTPQFEGEVRLGVP
                     PDIVTSLIPPVLQHFNQSWPRINVSLSCKASIVLLQDLADRKIDLTLTTEVDCGTGGE
                     TLLSDRLVWVGGPGSSAHLQRPLPVSLGSQLCRFRPEVIKALDAAQIDWRPVSQDCNM
                     EPVYATVKAGLAVAPLIRTSVPDYLDILGADAGLPPLPDFKVNLYLPVNATEIARELA
                     RHIRQGIELRFGPSRGRTERPQEHHEPVAATNIRATRPRPVQKRQVAAE"
     gene            1947..2135
                     /locus_tag="VFX46_00285"
     CDS             1947..2135
                     /locus_tag="VFX46_00285"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX5599054.1"
                     /translation="MSSLRFAPIAQLLSWLSLASRVYRERQALLALDERSLRDIGLSR
                     ADAYYEGSRAFWDIPQHR"
BASE COUNT          427 a          704 c          699 g          393 t
ORIGIN      
        1 agcagcggcc ccatcacgcc cggcgtgata accgtccggc cccactcgaa ccagcgcacg
       61 gcggaaccgg ccgcgagcga tgggctaggc gccgggataa gccatcggta cagcgcactt
      121 tccgccccca tgcgcgcgtg gaaccgcgtg cgaacgggcc ctggtgcaaa gacagccacc
      181 cgaacaccca gaccgcgtgt ctcccaggcg acggcttccg tcaatgcgat cacgtacgac
      241 ttgctggcgt agtaggccgc ttgatagggg ccgggtgcgt atcccccgag cgaggcgacg
      301 ttgagcacgc cgccacgtcc acgcacgcac atcgccggca agaagtgccg catcagcacc
      361 gacagggctc gaacgttgag ctcgatcagc tgaccaagct gctctggctc ttgactgata
      421 tagtcaccgc tcaagcccat gccagcattg ttaaccagaa catcggcgta gaagccgttc
      481 tggagcagcc tttcttggag cgcctgggga gcgtcaggcc gtgtcacatc gagcggcacg
      541 gtgagcacgg tcgttccgaa ctcttgacgg atggccgatg ccgctgtctc gagcggctcg
      601 gagcgacgtg cgacgagcac aactgcgcgc ccgcgcttgg cgaagcggcg ggcaatttcg
      661 aagccaatac cctccgatcc accggtcacc acgacggcag gctgaagctc tgcccactga
      721 cttagtgcgt cggggtccgg ccgccatcgg cggcggagcc atacgtcgac cagttgtgta
      781 aaactcattc ggctgcgacc tggcgcttct ggacaggcct cggacgcgta gcccggatgt
      841 ttgtcgctgc gacgggctcg tgatgctcct gcggccgctc cgtgcggccg cggctaggtc
      901 cgaagcgcag ctcaattccc tggcggatgt ggcgcgccag ctcccgtgcg atctccgtgg
      961 cgttgacagg caaatacaag ttgaccttga aatcgggaag cggcggcagg ccggcgtcgg
     1021 cacccaagat gtccaggtaa tcgggcaccg acgtgcggat cagcggagct accgccaatc
     1081 cggctttcac cgtcgcgtac accggctcca tgttgcaatc ctggctaacc ggccgccaat
     1141 cgatctgggc cgcatcaagc gccttgatca cctccggcct aaaacggcac agctgagagc
     1201 cgagcgagac cggaaggggc cgctgcaggt gggcgctgct gcccggtcca ccgacccaca
     1261 ccagtcggtc ggaaagcaag gtttcgccgc cggtaccgca gtcgacctcc gtcgtcagcg
     1321 tcaggtcgat tttccgatcg gcaagatcct gcaagagaac gatggacgcc ttgcagctaa
     1381 gcgagacgtt gatccgcggc caggactgat tgaaatgctg gagcaccgga gggatcagac
     1441 tggtcacgat atcaggcggc acgcccaggc ggacctcgcc ttcgaattgg ggcgtcgtca
     1501 tcgcacccca tgtcgcatca ttgagtgcga caagcctctg cgcattgcca aacaggcgct
     1561 caccagcggg tgagagcgtg aagcgcttac gctcgcgcac gaaaagctcc gtgccgaaca
     1621 ggtcctccag tcgcttgatt tggagactga cggctgcctg cgtgcgattg agaatgcgag
     1681 cagcggctgt cacgccaccg gtttcgacca cggcgaggaa ggcacgcagc aaggcgatgt
     1741 cgatatcgcg agtcatcggc atctcggagg ctcgcccatc ataaccaatc gtcatgcatc
     1801 acataagcat cattcgtttc cattatgcaa gtggagtctg gtagtcagtt caccacaacg
     1861 gggtggtcag cacctcgctg cacataagaa acggagttga gatgacgagc acaacacgga
     1921 gcgcatctcg aactcgcggc gggccaatga gctcgttgcg gttcgcccct attgcgcagc
     1981 ttttgtcttg gctgtcgctt gccagcaggg tctatcgcga gcgccaggct cttctggcac
     2041 ttgacgagcg tagcctgcgg gacatcggtc tcagccgcgc agatgcctat tacgaaggga
     2101 gccgcgcgtt ctgggacatt ccccagcacc ggtaatgcgc ttctgtgttg tgtgggggcg
     2161 gagtagacag ctccgccccg agctttttcc ggggccggga ggcggcgttc gacgttggtt
     2221 tgg
//
LOCUS       DASUMG010000018         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_82741, whole genome shotgun sequence.
ACCESSION   DASUMG010000018 DASUMG010000000
VERSION     DASUMG010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82741"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1584)
                     /locus_tag="VFX46_00290"
                     /pseudo
     CDS             complement(139..1584)
                     /locus_tag="VFX46_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
     gene            1882..>2441
                     /locus_tag="VFX46_00295"
     CDS             1882..>2441
                     /locus_tag="VFX46_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX5599055.1"
                     /translation="MPVSYTPRDWSSQPRYIDPAYKSTALRGPTKPLIPIPQSLSELT
                     GPVYGHESVGPLDSDLTRNGRRNGEPLGERIIVTGRVLDEMGRPVPNTLVEVWQANAA
                     GRYIHKADQHDAPIDPNFFGGGRCVTDAEGRYTFMSIKPGAYPWGNHHNAWRPSHIHF
                     SLFGPSFVTRLVTHMYFPGDPLLDLDP"
BASE COUNT          504 a          763 c          707 g          467 t
ORIGIN      
        1 acgagcggct ggcgcttcga ggtggtggat cttgacgggc ggcggatcga caaagtgctg
       61 gcgagccgca gtcttcgcag tcggcgcagc gcgctggcgt gagacgcgaa gggcgcacca
      121 agcatcgcga ttgagagttt acttcgtctc ctccggcgca cggtgataga cgtcatccgc
      181 ccgtagaatg tcgtcctcgc ctaagtaact gccgatctgc acctcgatga gctcaagcgg
      241 cacctagccg gggttttcca gccggtgcca ttgagttgcg acgatgtaaa cgctctcatt
      301 ctcgcggacg agcttttcct cctcgccgat ggtgaccttc gccgtgccgt gcaccacaac
      361 ccagtgctca gagcggtgat gatgcatctg catggaaagc ttgccgcccg gcttcacgtg
      421 cagcagcttc acctggaagc gggggcccag tgaaagtgtc tcgaaatagc cccagggccg
      481 ataactgcgc agatgttgct catgctcctt gcggtttgcg agcttcagcc gcgacacgat
      541 ctttgaaacg tcctgcgcgc gcgatttgtc ggcgacgagc agcgcatcgg gcgtgtcgac
      601 gataacgaga ttttcgacac ccaacgcaga aacgagcgcc ttctcgctgt ggacatagca
      661 gcctgctgca tcttcgagaa ttgcctcgcc acggctcacg tagttgccct gttcatcgtg
      721 tggcgcaatc tcccacagcg ctgaccatga gccgacgtcg ttccagccga aatctacagg
      781 aaccatagcg gccaaggctg ttttctccat gaccgcgtag tcgactgaaa tgttcggaca
      841 ctgcgcgaat gcagcttggt cgagccgtag aaagccgatg tccattgccg ctcgatcgag
      901 cgccagttga gcgaattccc gaatctcagg cgcatgccgc tgcagctctt cgaggaaagt
      961 cctcgcgtgc agaacgaaga tgccgctgtt ccagaagtaa tggccatcct cgagataaag
     1021 ctgagctgtc actgcgtcgg gcttttccgt gaacgccgca actttgaatg cgctcccctc
     1081 gaattgttcg agcggcttac cacggcggat atacccatag ccggtatgcg ggctggcggg
     1141 cttgatcccg aacaagacca accggccgct ggcggccacc tcgcctgcca agcgcaccgc
     1201 atcgagaaaa cgtgcctggt ccgctatgat atgatccgac ggcatcacga cgagaatgcc
     1261 cgaggaatcc tcctgcgcga tgctcagggc agcaacagcg atagcgcccg ccgtgttgcg
     1321 ctgcacaggc tccaaaataa tggcgcgcgc cgtcgtcccg tggctttcga ggttctcctt
     1381 gaccaaaaag cgatggtcgg cagtgcagag gattgtcggc ggctggaagc cttcgtcgga
     1441 cttcagcctt tccaaggttg cgccgagcag acttacttgg cgcccattga agcggatgaa
     1501 ctgctttgga tacatggccc gcgacaaggg ccagagccgc gtcccagatc caccggagag
     1561 gacaacgggg taaagagcgc tcatgcgcaa acctcttgga ccaccccgag ggaagccaaa
     1621 caagtttcac ggcctacctg ggtttcctat cagtcgacga tgcgtccctc aagtcgtgca
     1681 gggcgaatgc gctggtcatt cgggcgccgg gcctgcccgc ctcggccgca tcgatcaaca
     1741 cggaacttac cttgggctta tggatcgagg ccaccattcg aaatcgccgt gcaagcgacg
     1801 caacaacaag cgggcttgtg ctcggctccg caacctgatc tagaatcagc tcacgtcaga
     1861 aagaaaatcg ggaggagaac cgtgccggtc agctatacgc cccgcgactg gtccagccag
     1921 ccccgttaca tcgacccggc atacaaatcg actgcgctgc gcgggccgac gaagccgctg
     1981 atccccattc cgcaatccct ctcggagctg acgggcccag tctacggtca cgaaagcgtg
     2041 gggccactcg acagcgacct tacacgaaac ggacggcgta acggtgaacc gctgggcgag
     2101 cgaatcatcg tcaccggacg tgtcctcgac gaaatggggc ggccggtgcc gaacactctc
     2161 gtggaagtgt ggcaggccaa cgccgccggt cggtacatcc acaaggctga ccagcatgac
     2221 gcgccaatcg atcccaactt cttcggcggc ggccgctgtg tcactgatgc cgaagggcgg
     2281 tatacattca tgagcatcaa gccgggcgcc tatccctggg gcaatcacca caatgcttgg
     2341 cgaccgagtc acattcattt ctcactcttc ggaccaagct tcgtcacgcg tctcgtgact
     2401 cacatgtact tccccggcga tccactgctc gatttggatc c
//
LOCUS       DASUMG010000019         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_564611, whole genome shotgun sequence.
ACCESSION   DASUMG010000019 DASUMG010000000
VERSION     DASUMG010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564611"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFX46_00300"
     CDS             complement(<1..386)
                     /locus_tag="VFX46_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HEX5599056.1"
                     /translation="MKFHRLKHRAHQMLELRCSDPKSLVVGLAAALLSLSVAVSETMA
                     QTGSIKRPDSAQSSPVGVWFDDTGQGAIEIAPCGEMLCGRIVWLKEPLTKDGQPLTDA
                     LNPDPNRRDRPICGLQIIGDLKRQPNG"
     gene            668..1801
                     /locus_tag="VFX46_00305"
     CDS             668..1801
                     /locus_tag="VFX46_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5599057.1"
                     /translation="MILTLAIRNLFHDRVRLIVTLVGILFSIVLVAVQLGLYLGARKM
                     IIDMIENANGELWITAYGAKSFEEGGIVLTPRERHAALAAPGVAAVVPLIVSFAEWRK
                     PGGGSTTTVVVGSDAQDNGLKAWNIVEGSIATIEAPDGVAVDRSYLEDLGISGIGDVA
                     QIERGRVRVRALTEGIRSFTMAPYIFTTLNRARSLLGVPGENATYYLVQVKPGTDIEA
                     LRKDLIARLGPNTEVLTKAEFRDRSLDQWLFSTGAGVALIGGAILGLLVGTVIVAQTL
                     YSSTKDHLNEFATLRALGSSSGYIHRVILAQAGLSAVIGYVLGMIIALTVVVLSTHTP
                     LPIIMTPGLAASLFLLTLFMCAVSAISAIMKVTKIDPAMVFSR"
     gene            1798..2550
                     /locus_tag="VFX46_00310"
     CDS             1798..2550
                     /locus_tag="VFX46_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599058.1"
                     /translation="MSGPVVLEAVNIVKELGEGAGRVLALKGVSLSLRAGELTLLMGP
                     SGSGKTTLLSILGCILTPTSGSVRINGETLDDLSPEKLADIRRRHIGFIFQSYNLFPT
                     LTALENVRLALDVRGFSHAETVAKAEAALKAVGLGHKLRSYPRNMSGGEQQRVAVARA
                     IAGAPSVILADEPTAALDSENGHAVMALLAQIAKERNHAVLVVTHDPRTHAYADRIIR
                     IEDGLIVGEDRTPEAVKNIRKYDITRKRRANA"
     gene            2543..>2875
                     /locus_tag="VFX46_00315"
     CDS             2543..>2875
                     /locus_tag="VFX46_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5599059.1"
                     /translation="MPRIDSTFSTLVIVAVLAAGVGYGVNEWTEARKVEQRTSSLAAV
                     AQTEPAKPRWAASAPGQVEPRGGELRLTAQVPGRVAEVLVGANDRVAAGEVLILLDED
                     EARARLAAA"
BASE COUNT          534 a          845 c          925 g          571 t
ORIGIN      
        1 ccgttcggct gacgcttgag gtcgccaatg atttgcaggc cacaaatagg acggtctcgc
       61 cggtttggat cgggattgag tgcatccgtc agcggctgcc catctttggt tagcggctct
      121 tttaaccaaa cgatgcggcc gcagagcatt tcgccgcagg gggcaatctc gatcgctccc
      181 tggcctgtat cgtcgaacca aacgcccacc ggactggatt gtgccgaatc cgggcgtttg
      241 atcgagcccg tctgcgccat cgtttccgac accgcgaccg acagcgagag cagcgctgcg
      301 gccaagccta caactaggct ttttgggtcg gaacatcgca attccagcat ctggtgggcg
      361 cgatgcttca ggcgatggaa cttcatcagc gatctccaaa cgctattgct ctattcggag
      421 ggacagtttg gtgccttggc accgccaaac tcatcaccta actgggcggc gaattcagaa
      481 accatgaata gcgaaggtcg atgctccctg tggctttaag gagtggcacg aaaatggcaa
      541 acgcggcggg tatgccggtg cccgcgcgcg aaagggatgc gcagaaaatg gtacgaaatg
      601 cggccgatcg taaccttggt tactcgatag ccgtttgaac ccggcttaga tcggatatgg
      661 aagttcaatg atcttaacgc tggctatccg aaatctgttt cacgatcgag ttcgtttgat
      721 cgttactctc gttggaattc tgttctccat cgtgctcgtt gccgtgcagc ttgggctgta
      781 tctcggcgcg cggaaaatga tcatcgacat gatcgagaac gcgaatggag aactttggat
      841 taccgcttat ggcgcgaaaa gcttcgagga gggtggcatt gtcctgaccc cgcgcgagcg
      901 gcacgccgca ttggctgctc ctggcgtagc cgccgttgtc ccgctcatcg tctcttttgc
      961 tgagtggcgc aagccggggg gaggcagcac cacgaccgtg gtcgtcggct cggacgccca
     1021 ggacaatggg ctgaaggcgt ggaatatcgt cgaaggctcc attgcgacga tcgaggctcc
     1081 cgacggcgtt gcggtggatc gcagctatct cgaggacctt ggcatttccg gtatcggtga
     1141 tgtcgcacaa atcgagcggg gcagggtgcg cgtgcgtgcg ctcacagagg gcattcgctc
     1201 gttcaccatg gctccctaca tcttcacgac gttgaaccgt gcccggtcgc tgctcggcgt
     1261 gccaggcgag aatgccacgt attatttggt ccaggtgaag cccggcacgg acatcgaggc
     1321 gctccggaaa gaccttatcg cacgcctggg gccgaacacc gaggtgttaa ccaaggcgga
     1381 gtttcgcgac cgcagcctcg atcagtggct cttctcgacg ggcgccggcg tggccctcat
     1441 tggcggtgcc atacttggcc tgctcgtggg cacggtcatc gtcgcccaga cgctctattc
     1501 cagcacgaag gaccatctca acgagtttgc gacgctgcgt gcgctggggt cttcatccgg
     1561 ctatattcat agggtgatcc tggcgcaggc cgggctgagc gccgtcatcg gctacgtgct
     1621 cgggatgatc attgcgctca cggtggtggt gctcagcacg cacacaccgc tgccgatcat
     1681 catgacgccg gggctcgcgg cctcgctttt cctgcttacg ctcttcatgt gcgccgtttc
     1741 ggccatatcg gcgatcatga aggtgaccaa gatagatcca gccatggtgt tcagtagatg
     1801 agtggtcccg tcgtcctcga ggcggtcaat atcgtaaagg agttgggtga gggtgctggt
     1861 cgcgtcctgg cgctcaaggg cgtcagcctg tcgcttaggg ccggagagtt gaccttgctc
     1921 atggggccgt ccggcagcgg caaaaccacg ctgctgtcga tcttgggctg catcctgacg
     1981 ccgacgtccg gcagtgtgcg gatcaacggc gagaccctgg atgacctcag tcctgagaaa
     2041 ctggccgata tccgtcgccg gcacatcggc ttcattttcc agtcctacaa cctgttcccg
     2101 acgctgacag ccctcgagaa cgtgcgtctc gccctagacg tacgtggttt cagccatgcg
     2161 gagacggtgg ccaaggcgga ggccgcattg aaggccgtgg gcctcgggca caagctgcgc
     2221 agctaccctc gaaacatgag cggtggagag cagcagcgtg tcgcagtggc ccgcgcgatt
     2281 gccggtgcac cctcggtgat ccttgccgac gagccgacgg ctgctctcga cagcgagaac
     2341 ggccacgcgg tgatggcgct gctcgcacag atcgcaaaag agcgaaacca tgcggtgctc
     2401 gtggtgacgc acgatccgcg tacgcacgcc tacgctgacc gcatcatccg gatcgaggac
     2461 ggactgattg tcggcgagga ccgcacgcca gaggccgtaa agaacatcag gaagtacgac
     2521 attacgagaa agcgaagggc caatgcctag gatcgactcg actttcagca cgctggtgat
     2581 tgtggcggtg ctggccgcgg gcgtcggcta tggcgtcaac gaatggactg aggcgcgaaa
     2641 ggtcgagcag cggacaagct ccctggccgc cgtcgcccag actgagcccg ctaagccgcg
     2701 ctgggcggcc tcggcgccgg ggcaggttga gcccaggggt ggcgagctcc gcctgaccgc
     2761 tcaggtgccg gggcgcgtcg ccgaagtgct tgtcggcgcc aacgaccggg ttgcggcagg
     2821 ggaggtgctc atcctgctcg atgaggacga ggcgcgcgcc cgcctggccg ctgcc
//
LOCUS       DASUMG010000020         2799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_163162, whole genome shotgun sequence.
ACCESSION   DASUMG010000020 DASUMG010000000
VERSION     DASUMG010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163162"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /gene="lepB"
                     /locus_tag="VFX46_00320"
     CDS             <1..327
                     /gene="lepB"
                     /locus_tag="VFX46_00320"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5599060.1"
                     /translation="RRIPQYEETLPNGVKYRVLDSEPNGPFDNTGVFKVPENHYFVMG
                     DNRDNSTDSRAQWGVGFVPFENFVGRAEIIFFSAAFDEPDAFRLYTPWLWPWDIRWER
                     FFTLVR"
     gene            334..1074
                     /gene="rnc"
                     /locus_tag="VFX46_00325"
     CDS             334..1074
                     /gene="rnc"
                     /locus_tag="VFX46_00325"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5599061.1"
                     /translation="MRRRTKRTELETRLGYTFKSRELFNRALTHASVRSQRGARHDNE
                     RLEFVGDRVLGLAVADMLFELHPDASEGELARRFNRLVRGQTCASVARKLGLGPYLIL
                     SESEAESGGREKETILADALEALLGAVFIEAGFETARKIVRSLWSDQIDAMPHAIPDP
                     KSALQEWAQGQGLSLPEYIEISRSGPDHAPRFVTEVHIAGREPARGEGPTKRQAEQAA
                     ASSLLAREGVRLEAIRRSDAGLKKSSHG"
     gene            1067..2005
                     /gene="era"
                     /locus_tag="VFX46_00330"
     CDS             1067..2005
                     /gene="era"
                     /locus_tag="VFX46_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5599062.1"
                     /translation="MADTEAVAGQVPEDQMRCGFIAVIGAPNAGKSTLVNALVGAKVT
                     IVSRKVQTTRIPVRGIVVEGTSQLVFLDTPGIFRPRRRLDRAMVEAAWAGALDADIVV
                     LLVDSLKGITEDVERILDRLGEARGLKVLVLNKVDRLADKSKLLTLTEELTKRVEFQR
                     VFMVSALTGSGLKDLKSYLVEVAPPGPWHYPPDELSDLTDRKLAAEITREKIYERLHE
                     ELPYSITVETTDWTTKRDGSVRIDQTIYVERESQRKIVLGKGGATVKQISMEARKELA
                     ELLGHPVHLFLYVKVREDWAEDPERYREMGLEFPKE"
     gene            2039..2782
                     /gene="recO"
                     /locus_tag="VFX46_00335"
     CDS             2039..2782
                     /gene="recO"
                     /locus_tag="VFX46_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX5599063.1"
                     /translation="MNWSDEGIVLSVRPHGETATVVELLTRQHGRHFGLVHGGRSRRM
                     RPVLQIGNHVEATWKGRLAEHLGYFTTELRKGYAASVMEDPLALTGLTSLCALARLLP
                     ERDPHPEIFEVTLFVLSYLNDNSIWPALYVRWELNLLAELGFGLDLKSCAATGTTENL
                     VWVSPKSGRAVSAAAGEPYRDRLLPLPQFLLGPDRGPVTNRDVLDGLKLTGTFLETRV
                     LLPRDMTMPEARHRLVSALTRESRKEEGG"
BASE COUNT          523 a          834 c          929 g          513 t
ORIGIN      
        1 cgtcgcatcc cgcagtatga ggaaacgctg ccgaatggcg tgaagtatag ggtgctcgac
       61 tctgagccca acggcccgtt cgacaatacc ggcgtgttca aggtccccga aaaccactac
      121 ttcgtcatgg gggacaatcg cgacaactcg acggacagcc gtgcccagtg gggtgtgggt
      181 ttcgtgccat tcgagaactt cgttggccgt gccgagatca tcttcttctc ggcggcattc
      241 gatgagcctg atgcattccg actctacacg ccctggctct ggccctggga tattcgctgg
      301 gaacggttct tcaccttagt gcgctgaacg gacgtgcgac gccgaacgaa gaggacggaa
      361 ctcgagacgc gcctgggcta cacgttcaaa agccgcgagc tattcaatcg cgcgctgacc
      421 catgcgagcg tccggtcgca gcggggggcg cggcacgaca acgagcggct cgagttcgtc
      481 ggagaccggg tgctggggct cgccgtggcc gacatgcttt tcgagctgca ccccgacgcc
      541 agcgaaggag agcttgcccg ccgcttcaat cgtctcgtgc gggggcagac ctgtgcaagc
      601 gtggcacgga agcttgggct ggggccttat cttatcctca gcgagagcga ggcagagagc
      661 ggcgggcgtg agaaggagac gattcttgcc gatgccctcg aggccctcct aggggcggtt
      721 ttcatcgaag ctggtttcga gactgcacgg aagatcgtga gatcgctgtg gagcgaccag
      781 atcgacgcca tgccccatgc catacccgat ccgaaatcag cattgcagga gtgggctcag
      841 ggccagggac tctcgctgcc tgagtacatt gagatatcgc gcagtggtcc cgaccacgcg
      901 ccgcgcttcg tcaccgaagt gcacatcgcc ggccgggagc cggcccgagg ggaggggcca
      961 acaaagcgcc aggcagagca agcagcggcc agctcgctgc tggcccgcga gggtgtgcgc
     1021 ttggaggcga ttcgccggag cgacgccgga ctgaagaagt catcccatgg ctgatacgga
     1081 agccgttgcg gggcaggtcc ccgaagacca gatgcggtgc ggcttcattg cggtgatcgg
     1141 cgcgccgaat gccggcaagt ccaccttggt caacgccctg gtcggggcga aagtgaccat
     1201 cgtgtcgcga aaggttcaga cgacccgcat acccgtgcgc gggatcgtgg tcgagggcac
     1261 gagccagctc gtttttctcg acaccccggg catcttccgg cctcgacggc ggctcgatcg
     1321 cgcgatggtg gaggcggcgt gggccggtgc actcgacgcc gacatcgttg tcctgctggt
     1381 cgattccttg aagggaatca ctgaggacgt ggagcggatt ctcgaccggc tcggcgaggc
     1441 gcgggggctg aaggtgctcg tgctcaacaa ggtcgaccgc ttggcggaca agagcaagct
     1501 tctcacccta acggaagagc tgacgaaacg agttgaattc cagcgggtct ttatggtttc
     1561 cgcgctgacc ggcagcgggc tgaaggactt gaagtcctac ctggtagagg tcgcgccgcc
     1621 cgggccttgg cattatcccc ctgatgagct ttcggacctt accgaccgga agcttgcggc
     1681 cgagatcacc cgcgagaaga tttacgagcg cctgcacgag gagctgccgt actccatcac
     1741 cgtggagacg acagactgga cgacgaagcg cgacggttcg gttcgcatcg accagacgat
     1801 atacgtagag cgcgaaagcc aacggaaaat tgtccttggg aagggcggcg caactgttaa
     1861 gcaaatctcg atggaggctc gtaaggagct ggcggagttg ctgggccacc cggtgcacct
     1921 gttcctctat gtcaaggtgc gggaggactg ggccgaggat ccggagcgct accgcgagat
     1981 ggggctcgag tttcccaagg agtgagttgc tgcctacggc cagcactacg atcgactcat
     2041 gaactggtcc gatgagggta tcgttctgtc cgttcgcccg cacggcgaga cggcaaccgt
     2101 cgtggagctg ctgacacgac agcacgggcg ccacttcggg ctcgtgcatg gcgggcgcag
     2161 tcgccgcatg cggccggtgc tccagattgg caaccatgtc gaggccactt ggaaagggcg
     2221 gcttgccgag cacctcggct atttcacgac agagctgcgc aagggctatg cggcctcggt
     2281 catggaagat ccgctggcgc tcacgggcct cacgtctctc tgtgctctgg ccaggctgtt
     2341 gccggagcgg gatccacatc ccgagatctt tgaggtaacg ctgttcgtcc tgagctatct
     2401 caacgacaac agcatctggc cggcgctcta cgttcgctgg gagctcaatt tgttggccga
     2461 gcttggtttc ggtcttgacc tcaagtcctg cgcggcgacg ggaaccacgg aaaacctcgt
     2521 atgggtctct ccaaagtccg gccgggctgt gtcggccgcg gccggcgagc cttaccgcga
     2581 ccggctgctg cctctgcccc agttcctcct cggccctgac cgcggtcccg tgacaaaccg
     2641 cgatgtgctc gatggtttga agctcaccgg cacattcttg gaaacgcgtg tgcttctccc
     2701 gcgcgacatg acgatgcccg aggcaaggca ccggcttgtt tcggcattga cacgcgagag
     2761 ccgcaaggag gaggggggct agacctcttc cgagagata
//
LOCUS       DASUMG010000021         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_725213, whole genome shotgun sequence.
ACCESSION   DASUMG010000021 DASUMG010000000
VERSION     DASUMG010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725213"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VFX46_00340"
     CDS             complement(<1..686)
                     /locus_tag="VFX46_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5599064.1"
                     /translation="MSANEMTGGEALARMIQAHEGGPMFGMGGFQLLPFYDAVRRLGL
                     AHYLINDERAGVFAADAYAKVSGRVGLADATLGPGATNLVTGLVEALNAGSPLVVLTG
                     NTHRDHSWKNMTQESRQLEILRPAVKEVIRIESIHRIPELMRRAFAVATTGRPGPVIV
                     DVPEDICHGTHGYEEGEFHADPAYQAAPALRCRPDARSLERAARMIADARRPIVLAGG
                     GVHISGAAEAL"
     gene            complement(703..1998)
                     /locus_tag="VFX46_00345"
     CDS             complement(703..1998)
                     /locus_tag="VFX46_00345"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5599065.1"
                     /translation="MITANLVLVAVFAALLVVGAPIAVSLGLAGFAAIAVGLDVYALQ
                     SVAQITYNSINKYPLIAIPLFVLTGAVFEKAGVAARLVTFMQAVVGPRRGGLALVAIL
                     VCMILGGMSGSGPADAAAVATIMIPSMRRAGYPDAFSASVIAAGASTAILIPPSLALV
                     IYGVLVPGVDLRALFAAGLIPGVLVGCALMIPTLLISRKHGFDDCEGQKRPPFWRSFW
                     EALPGLFAPVIILGGLRSGLFTPTEAAAVAAVYGFIVGVAYRAFDLKGILEVLAESAE
                     TTAVIMIIIALAGLFAFAGSTLGAFDQAANAILALTNDPTVVLCLVMLLILFLGMLLD
                     GPSIYLISLPLLYPVALKLGWNLTWFGVLMAVNIAIGQVTPPVAVNLFVTAKVANVPI
                     EATVRWVIWFVLAMAAVLVLLIIFPELALWLPMHLGYSV"
     gene            complement(1995..2501)
                     /locus_tag="VFX46_00350"
     CDS             complement(1995..2501)
                     /locus_tag="VFX46_00350"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5599066.1"
                     /translation="MAHEPGTDVPEIAPERGDRLLPIERLIGALAMAGICVISFANVL
                     VRYISNYSFAFTEEYSTFLMVLLTFVGASAAVARNSHMRVGFIADRGHLWRRFCRAAA
                     RLAALAMFGMIVWYGGWLTWDQYRFEELSGGLGVPTWIYTIWMPVLSVLIMGRILFAW
                     WREERASQ"
     gene            complement(2563..>3054)
                     /gene="dctP"
                     /locus_tag="VFX46_00355"
     CDS             complement(2563..>3054)
                     /gene="dctP"
                     /locus_tag="VFX46_00355"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5599067.1"
                     /translation="LFNDTFTALGANPTQMSWADAQPALSTGAVDGQENPLSIFTVAK
                     LHTVGQKYVTLWHYVADPLIFSVNKQVWESFTPEDQEIVRQAAVEAGKYGIEVARKGV
                     TPEDSSQLEEIRKLGVTITELTPEELKAFQEATKPVYEKWKARIGAELVEAAEKAVAN
                     RGK"
BASE COUNT          665 a          981 c          927 g          481 t
ORIGIN      
        1 agcgcctcgg cggccccgga aatgtgcacg ccgccgcctg cgagcacgat cggccgtctg
       61 gcgtcggcaa tcatccgtgc tgcgcgttcg agcgaacggg catcagggcg gcaacgcaaa
      121 gccggagcgg cctgataggc cggatcggcg tggaactcac cctcctcgta accgtgagtt
      181 ccgtggcaga tgtcctcagg cacgtcgacg atcacgggcc ccggccggcc ggtcgtcgca
      241 accgcaaagg cccggcgcat cagctccgga atgcggtgga tgctctcgat ccggatgacc
      301 tccttgacgg cagggcgcag gatctcgagc tgccgcgact cctgcgtcat gtttttccag
      361 gagtgatccc ggtgcgtatt gcccgtcagc accacaagtg gcgagcccgc attcagagcc
      421 tcgacgaggc ccgtaacgag attggttgcg cctggtccca acgttgcgtc ggcgaggcca
      481 acacgcccgg agaccttggc gtaggcgtca gccgcaaaaa cgcccgccct ctcatcgttg
      541 atgaggtaat gcgcgaggcc aagccggcgg acggcatcgt agaacggcaa gagctgaaaa
      601 ccacccatcc cgaacattgg gccgccctcg tgcgcctgaa tcatgcgcgc cagggcctcg
      661 ccgccggtca tctcgtttgc ggacattcaa aactcctgaa gttcaaaccg aatagccaag
      721 gtgcattggg agccaaagtg caagctctgg gaagatgatg agcagcacca gcacagcggc
      781 catggccaaa acgaaccaaa tcacccagcg caccgtcgcc tcgatgggca cattcgcgac
      841 tttggccgtg acgaagagat tgaccgccac aggaggcgtg acctggccga tggctatgtt
      901 gaccgccatc agcacgccga accaagtgag attccagccg agcttcagcg ccaccggata
      961 aagaagcggc agcgaaatga ggtagatgga tggcccatcc agcagcatgc cgagaaacag
     1021 gatgagcagc atcaccaggc agagcacgac cgttggatcg ttggtgagcg ccagaatggc
     1081 gttggccgcc tgatcgaatg ctcccagcgt cgagcccgcg aaggcgaaaa ggcctgcgag
     1141 cgcgatgatg atcatgatga ctgccgtcgt ctcagcagac tcggcgagga cctcgaggat
     1201 gcccttgaga tcgaaggccc gataggcaac gccgacgata aagccataga cggccgcaac
     1261 ggctgccgct tccgtcggcg tgaataagcc gctccgcaga ccgcccagaa tgatgacggg
     1321 ggcgaacagg cctggcagcg cttcccaaaa tgagcgccaa aatggcggac gcttctgccc
     1381 ctcacaatcg tcgaatccgt gctttcggct gatcaacagc gtcgggatca ttaaggcgca
     1441 accgacgaga acgccgggaa tgagacccgc agcaaacaag gccctgagat caacaccggg
     1501 cacgagcacg ccatagatga ccagtgccag cgatggcgga atgaggatcg ccgtcgacgc
     1561 acccgccgcg atgacgcttg cggagaaggc atcgggataa cccgcacgcc gcatgctcgg
     1621 gatcatgatg gttgcgacgg cggcggcatc tgccgggccc gagccggaca tgccaccaag
     1681 gatcatgcag accaggatcg ccaccagagc gagcccgccc cggcgcggtc ccacgactgc
     1741 ctgcatgaac gtcactagcc gtgccgcgac gcccgccttc tcgaatacgg cgcccgttag
     1801 cacgaacagc gggatggcaa tcagcggata tttattgatc gagttgtagg taatctgagc
     1861 aacgctctgc agcgcataga catcaaggcc aacagcgatg gctgcaaagc ccgcgagccc
     1921 gagcgataca gcgatgggcg cgccgacgac gaggagcgca gcgaaaaccg cgacgagaac
     1981 gagattggcg gtgatcattg actcgccctc tcctcccgcc accacgcgaa aaggatgcga
     2041 cccatgatga gaaccgaaag cacaggcatc cagatcgtgt agatccacgt cggaacgccg
     2101 agccctccgg acagctcctc aaaccggtat tgatcccagg tcagccaacc gccataccag
     2161 acgatcattc caaacatagc gagtgcggcg agtcgggcgg ccgctcgaca gaaccgacgc
     2221 cacagatggc cgcggtccgc gataaagccc acccgcatat ggctgttgcg ggccactgct
     2281 gccgacgcac cgacgaacgt cagcagcacc atgaggaacg tggaatactc ctcagtgaaa
     2341 gcgaacgagt aattgctgat gtagcgcacc agcacattgg cgaagctgat cacacagatg
     2401 ccggccatcg cgagggcgcc aatcaggcgc tcgatcggca acaggcggtc gcccctctct
     2461 ggtgcgattt cgggcacgtc agtgcctggt tcgtgtgcca tgggagctgg cgcaaagcta
     2521 acgagcacac ccgccgcctc gggcaggtgt gctcagagac gactacttgc cgcgattggc
     2581 gacggccttc tcggcagcct cgacgagctc ggcgccgatc cgggccttcc acttttcgta
     2641 gaccggcttc gtcgcttcct ggaacgcctt gagctcctcg ggcgtcagct cggtaatggt
     2701 tacgccgagc ttcctgatct cctccagttg cgaggaatcc tctggcgtca cgcccttgcg
     2761 cgccacctcg atgccgtact taccggcctc cacagccgcc tgcctgacaa tctcctgatc
     2821 ttcgggcgtg aagctctccc agacctgctt gttgacggag aagatcagcg gatcggccac
     2881 gtagtgccac aacgtcacgt acttctggcc gaccgtgtgc agcttggcga ccgtgaaaat
     2941 cgacagcgga ttctcctgac cgtcgacagc ccccgtcgac agcgcaggtt gagcgtcagc
     3001 ccaactcatc tgcgtcgggt tggcgccgag ggccgtgaac gtgtcgttga acag
//
LOCUS       DASUMG010000022         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1046662, whole genome shotgun sequence.
ACCESSION   DASUMG010000022 DASUMG010000000
VERSION     DASUMG010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046662"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VFX46_00360"
     CDS             <1..450
                     /locus_tag="VFX46_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599068.1"
                     /translation="MAVGIANIIGTYVAGQSARFVEKRLGLSVIYFMRCFVFLGLLFL
                     PINGPVVILLSTMLGLFWLSTVPLTSGLVATFFGTTWMSMLFGFVFLSHQIGSFAGLW
                     LAGRLFDVTGSYDAMWWISVALGLFAAMVHLPIREQPVPRLAGSTVG"
     gene            518..649
                     /locus_tag="VFX46_00365"
     CDS             518..649
                     /locus_tag="VFX46_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599069.1"
                     /translation="MLRRAVVGGAVALILMGAGYLYAVRGEAILLDLAAMGSALLCL"
     gene            689..1180
                     /locus_tag="VFX46_00370"
     CDS             689..1180
                     /locus_tag="VFX46_00370"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEX5599070.1"
                     /translation="MQIFRAHRMLAFAGLALAAMMVVAAGSAAAQEIRTYSKKADYDD
                     VRFELTNAIINRGLVIDSTGQIGEMLERTGADVGSDKPIYKAAEFFTFCSAKLSRQMM
                     EADPRNIAFCPYVMFIYESVGEPGVVVVGYRRPSGSGSSETQAALAEIDSLLDGIARE
                     AVQ"
     regulatory      1231..1446
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1891..>2362
                     /locus_tag="VFX46_00375"
     CDS             1891..>2362
                     /locus_tag="VFX46_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX5599071.1"
                     /translation="MQDADESVAEAEAASNQIAAIPAAHLGTAVTVVTGDDLQRQQIR
                     HAADALRSLPGVSVNRTGSPAGLTQVRIRGAEANHTLVLIDGIEANAGSSGEFDFSDL
                     MIEDIERIEVIRGPQSALYGSNAIGGVVNIITKRRGGPLVTARAEGGSFGTKGGA"
BASE COUNT          405 a          684 c          767 g          506 t
ORIGIN      
        1 atggccgtgg gcatcgcaaa tatcatcggc acctacgttg ccggccagtc tgcgcgtttc
       61 gtcgagaagc ggctcgggct cagcgtcatc tatttcatgc ggtgtttcgt cttccttggt
      121 ctgctgttcc tgcccatcaa tgggccggtg gtgatcctgc tcagcacgat gctcggtttg
      181 ttctggctgt ccaccgtgcc cctgacgagc ggcctcgtcg cgacattctt tggaacgacc
      241 tggatgtcga tgctgttcgg gttcgtgttc ctgtcgcacc agatcggatc gttcgcagga
      301 ctgtggctgg ccggccgtct cttcgacgtg acgggctcct atgatgccat gtggtggatt
      361 tccgttgccc tcggtctgtt cgcggccatg gtccacttgc cgatccgtga gcagccggtc
      421 ccacgcctgg cgggctcgac ggtcggctga tgaaggtctc gcctggtctc aacgctttgt
      481 gttaggctga gcgccggaag gtgagtcaat ggatcacatg ctgagacgtg ccgtcgttgg
      541 tggagccgtc gccctgatct tgatgggtgc ggggtacctt tacgccgtcc gcggtgaggc
      601 gatcctgctc gacctcgccg caatgggcag cgccttgctt tgcctgtgac aggccgctgg
      661 gcggctgatc cccgacgagg aggtcgacat gcaaatcttc cgcgcgcacc gtatgctcgc
      721 cttcgcgggg ctggcgctcg cggcaatgat ggtcgtggct gccggctcag ccgctgcgca
      781 agaaatccgc acatacagca aaaaggccga ttacgacgac gtgcgctttg agctgacgaa
      841 cgcgatcatc aatcgcggcc tcgttatcga ttccacaggc caaatcggtg agatgctgga
      901 gcggacgggc gcagacgtcg gcagcgataa gccgatctac aaggcggctg agtttttcac
      961 cttctgctcg gccaagctgt ctcggcaaat gatggaagcc gatcccagga acatcgcctt
     1021 ctgcccatac gtcatgttca tctacgagag tgtcggcgag cccggcgtgg tcgtcgtcgg
     1081 ctatcggcgg ccctcagggt ctggaagttc ggaaacccaa gcggcgctgg cggagatcga
     1141 ttcgttgctt gacggcatcg cacgcgaagc agtgcagtaa gcatgctcaa cgccggcgtc
     1201 ccagcgcttg acggacgagg gcacctctga ttgtttggct ggcgtgccgg ttcctgtccg
     1261 ttatgcgggc gggtgaaaag ggaacacggt aacgggctgc gagagccccc aagccgtggc
     1321 tgcccccgca actgtaggcg gcgagcttcg ttcactgatg ccactggcca tattctcggg
     1381 ccgggaaggc tggacgaagc gtccgagccg cgagccagga gacctgccgg cacaggtcgc
     1441 ccatccggcg tgcggggcgc gcgacggagc ggatcagccg tagcggcgac tgatgtgtaa
     1501 ccctgtgggg gcacgtcaga attgcgtggt cgtcaggact tggctctgac tagcgtgccg
     1561 ccgctactgt ctggctggta cgctggtttt ctttcagagc tggctcgatg ccatttgcgc
     1621 tgcggtgctc ccacgcttac gccgtgcgga gcaaacatgc ctacacatac agttcgcagt
     1681 gcgtctctag ctcatcttct ttcctcggtt tcgtttatcc cgcttctcgc cgctggcatg
     1741 gccgcgccag ctaaggcgca gacgacgctc gaattgccgg gtattgttgt cgagggagcg
     1801 acattggaag cgcctcggcc cgggcccaaa agaaggcctg ctgcgacgac gccatcggca
     1861 ccgaatggag cttctcagca ggaagggcag gtgcaggatg cggatgaaag cgtcgcagaa
     1921 gccgaggccg cttcaaacca aatcgcagct attcccgcag cccaccttgg aacggcagtt
     1981 acagttgtga ccggcgacga tttgcagcgt cagcagatcc gccacgctgc agatgcgctg
     2041 cgcagtttgc cgggggtgtc ggtcaatcgg acgggatcgc cagccggatt gactcaggtg
     2101 cgtattcgcg gtgccgaggc gaaccacacg ctcgtgctta ttgacgggat cgaagccaat
     2161 gccggttcta gcggcgagtt cgatttttcg gatttgatga tcgaagatat cgagcgaatt
     2221 gaggtcatcc gtggtccgca gagtgcgctt tacggctcta atgccattgg cggcgtcgtt
     2281 aatatcatca cgaaacgtcg tggagggcca ttggtaacag ctcgtgcgga gggaggctca
     2341 ttcggcacga aaggtggtgc gg
//
LOCUS       DASUMG010000023         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1046759, whole genome shotgun sequence.
ACCESSION   DASUMG010000023 DASUMG010000000
VERSION     DASUMG010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046759"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1107
                     /gene="fabI"
                     /locus_tag="VFX46_00380"
     CDS             241..1107
                     /gene="fabI"
                     /locus_tag="VFX46_00380"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEX5599072.1"
                     /translation="MTEMDIAKPGPLMAGKRGLVMGVANERSIAWGIARVLAGHGAEL
                     AFTYQGEAFGRRAIPLAKSVGSKIIVPCDVSDIAEVDNVFAEIRDKWGSLDFVVHALA
                     FSDRRELSGRYADTSRENFINTMVISCFSFTEIAKRAAELMPNGGAMLTLTYGGSRRW
                     VPSYNVMGVAKAALEASVRYLASDFGPRGIRVNALSAGPMRTLAGSGISDARVIFNYQ
                     RDHAPLRRTPTLDEIGGAALYLLSPLAGAVTGEVHFVDCGYNTVAMPSLEALKEQEAD
                     AEAAGRTQPDGG"
     gene            complement(1283..1358)
                     /locus_tag="VFX46_00385"
     tRNA            complement(1283..1358)
                     /locus_tag="VFX46_00385"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1322..1324),aa:Glu,seq:ctc)
     gene            complement(1466..1849)
                     /locus_tag="VFX46_00390"
     CDS             complement(1466..1849)
                     /locus_tag="VFX46_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599073.1"
                     /translation="MAKADPVPPGASEPQFPTEHVARQVRILKFVVISLGVLLLVGFA
                     VVIGRIAYLATQPGGGIGASSPLHEMNVVLPAGAVIGQTTVSGDRMAVHYTSPAETGI
                     IVINLTTGRVVSRIGFTPEASGQQR"
     gene            1882..>2077
                     /locus_tag="VFX46_00395"
     CDS             1882..>2077
                     /locus_tag="VFX46_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX5599074.1"
                     /translation="MTRQPERLRVEVPAEAAGERIDRFLASRLEDLSRSRIQALIREG
                     RVRDRRGTIEDPGARVKPGEE"
BASE COUNT          407 a          625 c          662 g          383 t
ORIGIN      
        1 tgccttcgag gacatcccaa tcgtgcgcaa gcggctcgac ggcgtggaaa taaactgcgt
       61 tatgtccaac agctttggtt ttggcggcac gaatgccact ttggtgttcc gccgcgttga
      121 tacctagcac ccatgtcgca tcccaggccg gcataagttt cggaatagcc gggcatgcgg
      181 tttcgaaagt cagtgattgg agaatgatgc gccgctgtgc gcacgacatc aggagctccc
      241 atgaccgaaa tggatatcgc caagccgggt ccgctgatgg ccggtaagag agggcttgtc
      301 atgggagtcg ccaacgagcg ctccatagcg tggggaatcg cccgcgtgct ggccggtcat
      361 ggcgctgagc ttgcgttcac gtaccagggt gaggccttcg gccgccgtgc catacctctg
      421 gccaaatcgg tcggttcgaa gatcatcgtt ccgtgcgacg tcagcgacat cgccgaagtc
      481 gataacgttt ttgccgagat cagggacaag tggggcagcc tggacttcgt ggtgcacgcc
      541 ctcgccttct ctgaccgtcg cgaactatcc ggccgctacg ccgacaccag ccgcgagaat
      601 tttatcaaca ccatggtgat ctcctgcttt tcgtttacgg agatcgccaa gagggcagcg
      661 gagctcatgc ccaacggcgg tgccatgctg accctcacct acggcggctc tcgccgctgg
      721 gtgccttctt acaatgtgat gggcgtcgcc aaggcggcgc tggaagcgtc tgtgcgctat
      781 ctggcctcgg actttggccc gcgcggcatt cgggtgaacg cgctttcggc cggtcccatg
      841 agaacgctgg cgggatcggg gatttcggat gcgcgcgtca tcttcaacta ccagcgggat
      901 catgccccat tgcggcgcac gccaacgctc gatgagattg gcggcgcggc gctctacctc
      961 ctctcgccac tcgcgggcgc cgtcaccggc gaagtgcatt tcgtcgactg cggctacaat
     1021 accgttgcta tgccgagcct ggaggctctc aaagagcagg aggccgacgc ggaggctgcg
     1081 ggacgtacgc agccggatgg cggctaagaa gcaactctca gctgtagtgc ggcagagctg
     1141 gatgactcgc cagcttgcgg ctggagggag aggttccggc aattccggtc attgaagttg
     1201 gcttggggcg gtatcggcgt gaacaatttc gcgcgattct cgcgcggatc atcatcccca
     1261 agactgagct gcggaaggtt gttggcgccc cgtaggggac tcgaacccct gttaccaacg
     1321 tgagaggctg gcgtcctagg ccactagacg aacggggcag ccgatagtga tgctatagga
     1381 aaccgcgccc ggccaatcaa gcggctaaat ggcctagccg tggatgattc tcccggctaa
     1441 gccccctgta tctcgatgga cagcatcacc gttgctgccc cgaagcttca ggcgtaaagc
     1501 cgatacggct gacaacgcga cccgtcgtca gattgatgac gatgatgccc gtctccgctg
     1561 gtgacgtgta atgcacggcc atccggtcgc ccgagacggt agtttggccg atgacagcac
     1621 cagccggcaa aaccacattc atttcatgca acggggacga agcgccgatg ccgccgccgg
     1681 gctgggttgc gagataggcg atccgcccga tcaccacagc aaaccccacc aggagaagaa
     1741 caccgagcga aatgacgacg aacttgagaa tccgtacctg ccgtgcgacg tgctcggtcg
     1801 ggaattgcgg ctcggaagct ccgggcggga ctggatcggc cttcgccatg aacgtcaacc
     1861 ctcgatcggg aaaggatctc gatgacgaga cagccggaac gcctccgcgt tgaggtaccc
     1921 gccgaggcag ccggcgagcg catcgaccgc ttcctggcgt cccggctcga ggacctaagc
     1981 cgctcgcgca ttcaggcgct gatcagggag ggccgagttc gcgaccggcg cgggaccata
     2041 gaggaccctg gcgcaagggt caaaccgggt gaagagc
//
LOCUS       DASUMG010000024         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_163389, whole genome shotgun sequence.
ACCESSION   DASUMG010000024 DASUMG010000000
VERSION     DASUMG010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163389"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFX46_00400"
     CDS             complement(<1..302)
                     /locus_tag="VFX46_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX5599075.1"
                     /translation="MTDIHLNAAFPQSGHNHHACIALAIERAERAFAAKGLRLTEQRR
                     RVLEEIAASHHAVGAYEILERLAQKGKRLAPISVYRAIDALLDVGVVHRLESKNAF"
     gene            complement(431..1018)
                     /locus_tag="VFX46_00405"
     CDS             complement(431..1018)
                     /locus_tag="VFX46_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599076.1"
                     /translation="MSEAMMGRLAIGVAVLAVIAAIWQYSAATSAHDRAAAAEAARSA
                     LQSQLENVIAAREASDRALEEAKAQVKAAEEAKKDLEAQLSSAKEAQAAAEKTVEELK
                     SQLAEAQAAQQAAETKARGAADQVKAALEKAEAARSAAEQEAQELRAKLAEAEKARDA
                     AIQEAKEAGEQLAKERQARQAAEQALQNASRSVVQ"
     gene            complement(1234..1548)
                     /locus_tag="VFX46_00410"
     CDS             complement(1234..1548)
                     /locus_tag="VFX46_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015633514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5599077.1"
                     /translation="MATFIMLTRLSHQAIQSPASFKELSHKVMERIRKDCPEVEWKKS
                     FVVLGPADYLDIFTAPDIESAMKVATVIRTFGHATTEVWAATEWDRFVDLVSPMAGSG
                     GT"
     gene            complement(1622..2521)
                     /locus_tag="VFX46_00415"
     CDS             complement(1622..2521)
                     /locus_tag="VFX46_00415"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5599078.1"
                     /translation="MTLSQQLRSHAEAVEDRLQHILNSLPSEVPGRLADAMRYAVLGG
                     GKRFRPFLVLESARLFGLAIDDALATAAAVELVHCYSLVHDDLPAMDDDELRRGRPTV
                     HIAFDEATAILTGDALLTLALEVLAGAETHPDPIVRGELTLGLARAAGAAGMVGGQQL
                     DLEAERLPRQTTHDLQHVRRIQRMKTGALIAFSAEAGAILASAPIEDRVALANYGQAL
                     GAAFQIADDLLDVEGTAETVGKSTGKDAAAGKATYVAALGIEGARERLRELEAEAQSH
                     LNRFGTRAEILRQAVAYAATRTR"
BASE COUNT          474 a          823 c          783 g          554 t
ORIGIN      
        1 aaaaacgcgt tcttgctctc gagccggtgg acgacgccga cgtcgagcag tgcgtcgatg
       61 gcgcgataga ccgaaatcgg agcgagtctt ttgcccttct gagccaacct ctcgaggatt
      121 tcgtaagcgc ctacagcatg atgcgaggca gcgatttcct ccaacacgcg gcggcgctgc
      181 tcggtgagac gcaatccctt cgccgcaaaa gcccgctctg cccgctcgat cgccaaagcg
      241 atgcaggcat gatggttatg gcctgattgt gggaaggcgg cgtttaggtg tatgtccgtc
      301 atgatagtcg caggttcagc ctctcatcaa aatgtaatgt tatcacgttt cattctgaac
      361 ttgctatacc caccttttcc agattgccga gggcggctga cgtcgccctc ggaaaaattt
      421 ctgcaatcgc ttactgaacc acggaccgcg acgcgttttg gagggcctgc tcagccgcct
      481 ggcgggcttg gcgctccttt gctaactgct ctccggcttc tttggcctcc tgaatggccg
      541 catcacgcgc cttttccgcc tctgcaagct tggcgcgcag ctcttgcgcc tcttgctcag
      601 cggcggagcg cgcagcctcg gccttctcga gcgcggcctt tacctgatcc gcggctccac
      661 gcgctttcgt ctctgcggcc tgctgcgcag cctgggcttc ggccagttgg ctcttcaact
      721 cttctacggt tttctcagcg gcagcctgcg cctcctttgc actggagagc tgagcttcga
      781 ggtccttctt ggcctcctct gcagccttga cctgggcctt tgcctcctca agtgcccggt
      841 cggaggcctc gcgggcggcg atgacatttt ccagctggct ctgtaatgcc gatcgcgctg
      901 cctctgcggc ggcggcgcga tcgtgcgcgg acgttgcagc gctgtactgc cagattgctg
      961 caatcactgc cagaacggct acaccaatgg ccaatcggcc catcatcgct tcggacatgg
     1021 tcgttttctc cccagctcga tggctgcggg cagtgtaggg caacacggat caaaaatcat
     1081 tcttccactc gttgcgccgg agcccggccg ttacgtcagc gattgccggc ttgcggcgcc
     1141 aagggcatcg caaaggagat gctcacgcag gcttgctact gagggtaggg gacgcggggc
     1201 aaggcgggtg agcttgcccc ttactatgtt cgactaggtg ccgcccgaac ctgccatcgg
     1261 cgatacgagg tccacaaacc ggtcccactc cgtggctgcc cagacctcgg tggttgcatg
     1321 gccgaaggtg cggatgaccg ttgcgacttt catcgcagat tcaatgtcgg gcgcagtgaa
     1381 gatgtcgaga taatcagccg gcccgagcac gacgaagctc ttcttccatt cgacctcagg
     1441 gcaatccttg cggatgcgct ccatcacctt gtggctgagc tccttgaaag aggccggcga
     1501 ctgaatggcc tggtggctca gccgggtaag catgatgaac gtggccatga tggcatctcc
     1561 tatccgtcgt gagactggac aggaacgcca acgagtcagg cccggatccc cgcgggtgag
     1621 ctcaccttgt gcgtgtggcc gcgtaggcga cagcttgtcg caggatctcg gcgcgggtgc
     1681 cgaaccggtt cagatgcgac tgggcctctg cttccagctc tcgcaaccgt tcccgtgcgc
     1741 cctcgatacc gagcgccgcg acgtaagtag cctttccggc ggcggcgtcc ttgcctgtcg
     1801 acttaccaac cgtttcggcc gtgccttcca cgtcgagcaa atcgtcggcg atttggaatg
     1861 cagcacctaa tgcttgccca taatttgcaa gagcaactcg atcctcgatc ggagccgagg
     1921 cgagaatcgc gccggcttcg gccgaaaagg cgatcagtgc ccctgtcttc atccgctgga
     1981 tgcgtcgcac gtgctgcagg tcgtgcgtcg tttggcgggg gagacgctcg gcttcgagat
     2041 cgagctgctg accacccacc attccggcag ctcccgccgc gcgtgccaag cccaacgtta
     2101 attcgccacg cacgattgga tcgggatgag tctcagcgcc cgcaagcacc tcaagtgcaa
     2161 gcgtcagcag tgcatcgcca gtgaggatgg cagtcgcttc atcgaatgcg atgtgaacgg
     2221 tcggtcgccc tcgccgcaac tcgtcatcgt ccatcgcggg aagatcgtcg tgcacgaggg
     2281 agtagcaatg caccagctcg acagccgctg cggtcgcgag cgcatcatca atcgctaggc
     2341 cgaagaggcg agcactttcc agcaccagaa aaggacggaa tctctttcca ccgccgagca
     2401 ctgcgtaacg catggcgtca gcaagccggc ccggaacctc cgaaggcagc gaatttaaga
     2461 tatgctgcag ccgatcctcg actgcttctg catgggaacg gagctgttgc gagagggtca
     2521 tgccgacgcc attaaagaaa ggtttacgtc ccgagatcgc gcctggtgcc gtcgtgtgca
     2581 ggccacgcta taacacttcc cgtggagagc tggtccagaa gtatggcttt gcag
//
LOCUS       DASUMG010000025         4755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_163643, whole genome shotgun sequence.
ACCESSION   DASUMG010000025 DASUMG010000000
VERSION     DASUMG010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163643"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..501)
                     /locus_tag="VFX46_00420"
     CDS             complement(157..501)
                     /locus_tag="VFX46_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5599079.1"
                     /translation="MRRVREGGGPEALRRRKAPGGKHRLSREQHARLLDLLRQGAEAH
                     GFKGELWTRKRVAVLIAREFGVRYHPGHVSKILKSLDWSSQKPERRASQRNEAAIRQW
                     ARERWPEVNKAR"
     gene            1489..1701
                     /locus_tag="VFX46_00425"
     CDS             1489..1701
                     /locus_tag="VFX46_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599080.1"
                     /translation="MDLPSGALAAPGAREQGRTVLGEVSANLRQLDDLVRFQLANFES
                     AVERAVAVAALRRSMFDDLIDSLERE"
     gene            2717..3775
                     /locus_tag="VFX46_00430"
     CDS             2717..3775
                     /locus_tag="VFX46_00430"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HEX5599081.1"
                     /translation="MVRVWVLLLTLVAVQSNQMVFAQQCTRWASLELTEEWRYVPPED
                     GPSDRLIGRVAWADRDERGVSYVLDGLNNRVVILDSLGNFMKAVGRRGSGPGEFESPR
                     SIRVTSDGGFAVLDEGLSRVEIFSSDGSYERSVLFRTGLPNVRDFLVVDSFFVISGTT
                     AVSEAVVHLYDHEGNYVRGYGSVRKDLKEPVLIARYSDGVLARRSDGAIVFARRTPFQ
                     LQVFADDETLLDVTRTDVVPDYVSQVASRVGDGWRFNWRHPALTSIFALPDDCYLVLV
                     QELPADAEDETEYQTVLHVLDRNGIPVVKKKLEPFVWATKYWRDARGDHILSVSIDRS
                     TGIMYPVQYRIDRTTSAR"
     gene            complement(4248..4622)
                     /locus_tag="VFX46_00435"
     CDS             complement(4248..4622)
                     /locus_tag="VFX46_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599082.1"
                     /translation="MSCLLLVPLIACGDDDDGTGPTPTPVATTVTIDPGTATLTAIGD
                     TIRLAARVRDQNGATMAGATVTWSSLDPDVAPVAGAGSTRGIETRANCNPVYLFVECS
                     LSGSNDAENNLRSTRESRAITI"
BASE COUNT          887 a         1323 c         1504 g         1041 t
ORIGIN      
        1 cctcgagcga aaccgcccct ctcaccgaga ggtggtcgtg gctgagtttg gagcgaagca
       61 gcagcggcgt ttctcccacc ggtgcgtacg tgcgcacgac cgagggcagc agatagaagc
      121 cgctctcgtc tgccaagagc agcgtgcggc cttccgctat cgcgctttat ttacttctgg
      181 ccagcgctca cgcgcccact gccggatggc ggcctcgttg cgctggctgg cccgccgttc
      241 gggcttctgt gagctccagt caagcgactt gaggatcttg ctcacgtgtc ccgggtggta
      301 ccgcacaccg aactcgcggg caatcagcac agcgacacgc ttcctggtcc aaagctcgcc
      361 cttgaagccg tgggcttcgg ctccctggcg gaggagatcg agcaggcgag cgtgctgttc
      421 gcgcgaaagg cgatgtttac cacccggtgc tttgcgccgc cgcagtgcct cggggcctcc
      481 gccctcacgc acgcgccgca tccactgcga gacggcgccg ggggtgacac ccacagtgct
      541 tcggcgatct tgctctggct ccagcccgac tggaagagct cccaggcgcg cagccggcga
      601 ccctcgcgct aggccgtcgc ctgcgacgag agtgcgacgc ccatgtttca cctccgctga
      661 agagtgcagc ggcggtgcgt tagcaaatca cacgccggtc agtaacagat ccgcccacat
      721 ccgggagagt tgggccattg tcgtgaagcg gcggaggtta cgtctgcccg agccttgtac
      781 cctaatcccg gacccttttc gctgtcatgg agctggaagg ctgggatgct tgcgctggag
      841 gcttgggtgg acaacaagag catgcatcgc cagggatggt caatccgtgg gatcgccaag
      901 gagttggacg tcggtttaaa catcgtgcgg cggtatcttc gcgaagagac gccgtccgcc
      961 tacaggccgc gcccaaagag gccgagcatt ctcgatccgt acaaggacta cctgagagcg
     1021 cgcctcgagg acaagccgga gatctgtgcg aaggtgctgc ctagcgaacc cccggctcgg
     1081 ggctacccag gcgtaggtta cgctcatcaa ggatttcatc cggccgcttc gtgtcgagca
     1141 gaggcgactt gtggagcaga cggtaatata ttgagaccgc accaggcgag cggctccagg
     1201 tcgactggag tgagtttggt cgactgccct gcggccgcaa gctctacggg ctgtcctatg
     1261 tgctcagctg gtcgacgacc ctcttcgtct actccaccac acgcacggtc ggccaggagc
     1321 tccttttcga caacccgaac acggtgatgc gccgagcgaa ggggcaggca cgtcaagcag
     1381 gtccgtgtag aaggcatcga ggtcacgccg gttcagccgg aagcggctga tcagctcggc
     1441 gtccttggcg accagctgcg gaccgaagtg gctaaacgtg agcgctcggt ggatctgccc
     1501 tcgggtgcgc ttgccgcacc gggggcacga gagcaggggc gcacggtgct cggtgaggtt
     1561 agcgcgaatc ttcggcagct cgatgacctg gtgcgctttc agctggctaa cttcgagagc
     1621 gccgtcgagc gagcggtggc agtggcggca ctccgcaggt cgatgttcga cgacctcatc
     1681 gactcgcttg agcgggagta gactgcgcct gctagcctgg tgccccttct ggccgcccca
     1741 cattcttcca ctcgcctgtc gcctgtggcg agcggtgccc ggtgagtcga aatagggcgg
     1801 cttggacaag ttggtggagt tcgtcgcgag ccgcgcctcg agttctttga ccttggcctc
     1861 acactcgtgg gctcaggtct cgagcgtgac aaggcccgca cctaccacct gcgagaggat
     1921 ctcgcgcgcg acctcgagtt gctcaatatt gttcctgaag cttcaacttt atgcaagtgt
     1981 tcatcgtgca gacggttgtc ggcaggaaga ggtgctttgt gatccatcgc atattggcat
     2041 cgggtgcggc gggacgttgg cgaagaaggc ttgactacaa cgggacgctt acgggcgcaa
     2101 agggtttgac atgatgagcg gacggtgcta aacagctcga atgtgacacc cggagatttg
     2161 ctatcaaaga ccccgggctc ccaatcgtca attccacgtg ccattcggca ccgcctacac
     2221 tcctgcccaa agctttccac gggcggtttg gttgtgatag atcttcggcc ggtgtgctct
     2281 ttagatttgt aaaggtgcgc tagtcagatc tactgtgtgc ccgtaacatc gcttggcggt
     2341 aattgagcgt ttgactgaac tcatggactt actccattgg ttcaggcgcc ataattgagg
     2401 gtcgtatagc caaacacgca cgttgtgtct ttccagcgta tggctcaggc ggttcttcta
     2461 ccgtccgttt gggattgacc cgtacggtag ccccgttctg cctggccatg acgaccacat
     2521 ggcactgact gcgcgctagc ccaacgaggt cacgctcctg gttctcgtaa tacccatagc
     2581 cgcgttgtca ttagacgtca ttgtattgcc gtgctgtgat tcggtggttg atcacaaaga
     2641 ccggattgta gtcaacgttg cgccgttcgt gctgtggtaa agttgcaatg tgaccttttt
     2701 ctcggagggg acagcgatgg tccgagtgtg ggtgctgctc ttgaccctgg ttgctgttca
     2761 gtccaatcaa atggttttcg cgcagcagtg cacacgttgg gccagcttag agttgacgga
     2821 agaatggcga tacgttccgc ccgaggacgg tccttccgac cgactgatag ggcgagtagc
     2881 ctgggcagac cgcgatgaac gtggagtaag ctacgttctc gatggtctga acaaccgcgt
     2941 cgtgatcctg gatagtttag gcaatttcat gaaggcggtt ggtcgcaggg gttcggggcc
     3001 cggagaattc gagagccccc gaagcattcg cgtgacttca gacggcgggt ttgccgttct
     3061 cgatgaaggc ctgagccgtg tggagatctt ctcgtcggat ggatcgtacg aaagatccgt
     3121 cctcttccga accggactcc caaatgtacg tgattttctc gtggttgatt cgtttttcgt
     3181 catttccggg acgaccgccg tctctgaagc cgtcgtgcac ctttatgacc atgaggggaa
     3241 ttacgttcgc ggctatggaa gtgtcagaaa agacttgaag gagccggtcc tcatagctcg
     3301 ttacagtgat ggggtgctgg cccgacgttc cgacggtgcg atagtcttcg cgcgacgcac
     3361 gccatttcag ttgcaggtgt tcgctgacga tgagactttg ctggatgtta cgcggacgga
     3421 cgtcgttcca gactacgtga gccaggtggc atcgcgagtt ggtgacggct ggaggttcaa
     3481 ttggcgccat ccggcgctga cgagcatatt cgcgctgcca gatgattgtt acctcgtgtt
     3541 ggttcaggag ctgccggccg acgccgagga tgaaaccgag tatcagaccg tgctgcatgt
     3601 attggatcga aacgggatac ccgtggtgaa aaagaagctt gagccgttcg tttgggcgac
     3661 gaaatattgg cgcgatgcaa ggggtgatca tattctgtca gtgagtattg atcgctcaac
     3721 gggtattatg tacccggtcc agtatagaat agatcgcacg acgtccgcga gataattcct
     3781 ctaaggaggt tgtcatgggt aagttgctct cagcaggtct ggtcgctgtg gcgctgtttg
     3841 cgatgcccat ggaacttacg gcgcggtgtg cgaagtgcca tgatccatgg tttggcaagg
     3901 agcgctgcga gccctgttcg agtgggagta gctgtggggc tgcgtgcagt tttgatcacg
     3961 cgggtaattg ctatctcact gcgccttgcg gggcgagatt aagccaaaca ccattcctgt
     4021 actcgatcat ttagccgcta tgtcggtggc actcaattcg gatgcattgg agggaattgg
     4081 ggaagcggca cgtggtgagt tgatcgagga tggaatgcta aatgtaagtg atacgcgtcg
     4141 tgcgatcccg gccgtgggta tgagggatgc tccatactca cggccgattt ccattgtttt
     4201 cctatacctg cccacccgaa ttgatttgtt caatacaggc ggtcgcctca aatcgtgatc
     4261 gctctggatt cacgagtgct cctcaggttg ttctcggcgt cattcgaacc ggataatgag
     4321 cactctacga ataaatagac tggattacaa ttagcgcggg tctcaattcc ccgggtacta
     4381 ccggcccccg cgaccggcgc cacgtcaggg tcgagactgc tccaggtcac ggtggcgccc
     4441 gccatggtcg cgccgttctg gtcgcgcacc cgggccgcga ggcgaatcgt gtcgccgatc
     4501 gcggtgagtg ttgccgtccc ggggtcgatc gtgaccgtgg tcgcgacggg cgttggggtc
     4561 ggaccggtcc cgtcgtcatc atcgccgcag gcgatgagcg ggacgagaag gagacacgac
     4621 actatggagg tccggatttt cgtggcgcac cttgagggcc gatacagccg agaaacgtca
     4681 cacgacgacc agacttcggc tcgcgccgag tctcaaactg ctcaagcatc gggacctcga
     4741 gggtggacgg caatg
//
LOCUS       DASUMG010000026         4043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_3143, whole genome shotgun sequence.
ACCESSION   DASUMG010000026 DASUMG010000000
VERSION     DASUMG010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VFX46_00440"
     CDS             <1..298
                     /locus_tag="VFX46_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEX5599083.1"
                     /translation="GGLKITEPAADLAAAAALLSSISGVALPQNQVFFGEISLSGAVR
                     AAAHMTTRLKEAQKLGFRHAVLPQAGEVDETALTLSLQRIPHLKSLAEQLAPCN"
     gene            351..992
                     /locus_tag="VFX46_00445"
     CDS             351..992
                     /locus_tag="VFX46_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX5599084.1"
                     /translation="MIGPLTYLDAGLLAICFLSGLLAMYRGLTREILSILSWLAAAAA
                     VLYFVLFHRPFAEDLAQQVNVPVAIAQIAVGAVIFLIVLVVVHLITARISDTILDSRV
                     GMFDRILGFVFGVVRGFVLVVIPYMFYESFVPDPQQQVAWVREAKSLPMIRSTGETFR
                     IILSQYVPSSLTEPTSIDEQSATTIPKIQDVAVLYPSEIHISVTQTGSLADRG"
     gene            1059..2543
                     /gene="purF"
                     /locus_tag="VFX46_00450"
     CDS             1059..2543
                     /gene="purF"
                     /locus_tag="VFX46_00450"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX5599085.1"
                     /translation="MAGSDLVLDDPHWLRYGDDRLREECGVFGIIGHDDAAALTALGL
                     HALQHRGQEAAGIVTFDGQHFHSERRMGQVGDNFNKVDVIKRLRGQLAVGHVRYSTTG
                     ETILRNVQPLFAEFESGGFAVGHNGNLTNALTLRRELVQSGAIFQSTSDTEVLLHLIA
                     RSQKRRIVERFVDALRQLEGAYAFVCLTNDMLIGARDPIGLRPLVIGRLGPAYILASE
                     TCALDIVGAEFVREVENGEVVVITKKNGLESHRPFPMRPARPCIFEYIYFARPDSIVG
                     GRNVYEVRKRMGFELAQEAPADADVVVPVPDSGVPAAIGYAQASSLPFELGIIRNHYV
                     GRTFIEPEQRIRQLGVKLKHSANSAAIRGKRIVLIDDSIVRGTTSKKIVNMMREAGAR
                     EVHMRIASPEIKYGDYYGIDTPSRKELLAANLSLEEMCAFIGADSLQFLSVDGLYRAM
                     GYDGRDNINPQFSDHCFTGDYPTELTDLNGGSLPRQLSLLAEAG"
     gene            2670..3839
                     /locus_tag="VFX46_00455"
     CDS             2670..3839
                     /locus_tag="VFX46_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599086.1"
                     /translation="MVGKRCLAAVIFVLAAGAADQSFASRIHTGDETGTFHARFCPEL
                     VSILGQSELAYTCQASTGTSANVREVMASPSDLAYAQLDIFARELERLDGAGAIIPIR
                     KDDARQCLFAVTKRRELQSFGELSALAPELKFHLPPEDSNSSGTFKILQEIDAAGVGQ
                     AAAVQHEASAEDAIRAALASENAVAFFVEFPNPNDTRFTLVRELGGHFVPIIDREILR
                     HHIGGEKVYFAQETQVSNAEWLKSARKAVTACTPMVLFTGAPEKITEPKARQDHEDMI
                     ATVRALRTEMLLPQESTFARILARTKELSASSAEKLMQASERAKERARPYIQDAKEVT
                     ERAIEAAKPTLDKAKEYGRKFYEQAREGLKGLMDSNSETEPPAAQPEEPRNEPQQ"
     gene            3879..>4043
                     /locus_tag="VFX46_00460"
     CDS             3879..>4043
                     /locus_tag="VFX46_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599087.1"
                     /translation="MTKRLEGRIALITGASRGIGRAVALAFAREGAHVVLLARTQGAL
                     EELDDEIREQG"
BASE COUNT          762 a         1245 c         1231 g          805 t
ORIGIN      
        1 tggcggcctg aagatcactg agcccgcggc ggatctggcc gccgcggccg ccctgctctc
       61 gtcaatttca ggtgttgcgt tgccccagaa tcaggttttc ttcggggaga tcagcctttc
      121 cggtgcggtt cgggccgcgg ctcacatgac cacgaggctg aaggaggcgc agaagctagg
      181 cttccggcac gccgtgttgc cccaagctgg ggaggtggat gagacggcgt tgacgctttc
      241 tttgcagcgt atccctcatc tgaagagtct tgcggagcaa cttgcgccgt gcaactgaga
      301 tcgtggggaa tatcccacat gcctgaagaa gagttggggg agcctcgacc atgatcgggc
      361 cgctgacata tctcgacgcc ggccttttgg ccatctgttt cctatccgga ctgctggcaa
      421 tgtatcgcgg cctgacgcgg gagatcctct cgatcctgtc gtggctcgcc gcggctgctg
      481 cggtgctcta tttcgtgctc ttccacaggc ccttcgccga ggatctggcg cagcaggtca
      541 acgtgcccgt ggctatcgct cagatcgctg tcggggccgt gatcttcctg atcgtcctgg
      601 ttgtggttca cctgatcacc gcgcgtatct ccgacacgat cctcgacagc cgcgtcggaa
      661 tgttcgaccg cattctgggc ttcgtgttcg gtgtggtccg cgggttcgtg ctcgtggtga
      721 tcccttatat gttttacgaa tcgttcgtgc ccgacccgca gcagcaggtg gcgtgggtgc
      781 gcgaggccaa gtcgctgccc atgatccgga gcaccggcga gaccttccgc atcatcctat
      841 cgcagtacgt accctcttcg ctgactgagc cgacgagcat cgacgagcaa agcgcgacga
      901 caattcccaa gattcaagac gtcgcggtgt tgtatccgtc agaaattcat atatcagtca
      961 cgcagaccgg gagcctggcc gatcgcgggt gatgggcatg gcgctcggat ggcgtcattt
     1021 tcatcaacgt ggcggctcgg agactctcga tggcgagcat ggcgggttct gatctggtgc
     1081 tcgacgatcc ccattggctg cgctacggcg acgaccgcct tcgcgaggag tgcggcgtct
     1141 tcggcatcat cggccatgat gacgcggctg ctctgacggc actcgggctt cacgccctgc
     1201 agcaccgggg ccaggaggct gcaggcatcg tcacgttcga cggccaacac ttccattccg
     1261 agcgccggat ggggcaggtc ggggataact tcaacaaagt cgatgtcatc aaacggctgc
     1321 gcggccaatt ggctgtcggg cacgtccgct actcgaccac gggcgagacg attttgcgca
     1381 atgtgcagcc gctctttgcc gagttcgaaa gcggtggctt cgccgtcggc cacaacggca
     1441 atctgaccaa tgccctgacg ctccgtcgcg agctcgtcca gtcgggcgct atcttccagt
     1501 caacttcgga tacggaggtc ctcctccacc tcatcgcccg ctcgcaaaag cgccgaatcg
     1561 tcgagcgctt cgttgacgcc ctgcgccagc tcgaaggcgc ctacgccttc gtgtgtctga
     1621 ccaacgatat gcttatcgga gcacgcgacc ccatcggcct gcgcccgctt gtgattggtc
     1681 gtcttggccc ggcctacatc ctcgcttccg aaacgtgcgc gctcgatatc gtcggcgccg
     1741 agttcgtacg tgaggtcgag aacggagagg tcgtggtgat tacgaagaag aatgggctcg
     1801 agagccaccg cccattcccc atgcgccccg cccggccctg cattttcgag tacatctatt
     1861 tcgcccgccc cgattccatc gtcggcgggc gaaacgttta cgaggttcga aaacggatgg
     1921 gcttcgagct ggcccaggag gctccagctg acgctgatgt cgtcgtgccc gtgcccgact
     1981 ccggcgttcc cgcggccatc ggttatgccc aagcctcgtc actgccattc gagcttggca
     2041 ttatccgaaa ccactatgtc ggccgcacct tcatcgagcc ggagcagcgc atccgccagc
     2101 ttggcgtgaa attgaagcac tcagccaatt ccgctgcgat ccgcggcaag cgcatcgtgt
     2161 tgatcgacga cagcatcgtg cgcgggacga cctcgaagaa gatcgtcaat atgatgcggg
     2221 aggcaggtgc gcgagaggtg cacatgcgga ttgcctcccc ggagatcaaa tacggcgact
     2281 attacggaat cgacacgccg tcgcgaaagg agctgctggc ggcaaatctt tcgctcgaag
     2341 aaatgtgcgc gttcatcgga gccgattcgc tgcagttcct ttccgtcgat gggctgtacc
     2401 gcgccatggg ctacgacggg cgggacaaca tcaatccgca attcagcgac cattgcttta
     2461 ccggcgacta cccgaccgag ctgactgatc tgaatggcgg ctccctgccc cgccagttgt
     2521 cgctgctggc cgaagccggt tgatccgacg tcgctgttca ccactcaggc ttgcgccaaa
     2581 cgattgggat atgatctcct tcaggtggaa gcggtgtgcg cttgcactgg agcgtttgcg
     2641 cgtctgatca ggagaacggg aggcccatta tggttgggaa gcgttgcctg gccgcggtga
     2701 tttttgttct ggctgccggc gccgcggacc agagcttcgc gagccgcatc catacgggtg
     2761 atgagacggg aacgttccac gcgcgcttct gcccggagct ggtttcgatc ctcggccaat
     2821 cagagctcgc ctacacctgc caggcctcga cgggaacgtc tgcaaatgtc cgggaggtca
     2881 tggcgtctcc ctccgacctg gcctacgccc aattggatat cttcgctcgc gagctggaac
     2941 gcctggacgg tgcgggtgcg atcattccca ttcgcaagga cgacgcccgc caatgcctct
     3001 ttgccgtaac caaacggcgg gaactgcaaa gcttcggcga gctttcagcg cttgctcccg
     3061 agctcaaatt ccatttgccg ccggaggact ccaacagctc ggggaccttc aaaattctgc
     3121 aggaaatcga cgctgccgga gtgggccagg ccgcagccgt ccagcacgag gcgagtgctg
     3181 aggatgccat ccgcgccgcc cttgcgagcg aaaacgccgt cgcgtttttc gtggagtttc
     3241 cgaacccgaa cgatacccgg tttacgctgg tgcgtgagct gggcgggcac tttgtgccca
     3301 ttatcgatcg agaaatcctg cgccaccaca ttggcggcga aaaagtttat ttcgcccagg
     3361 aaacgcaggt ctccaatgcg gaatggctca agagcgcccg caaagccgtg accgcctgca
     3421 cgccaatggt gcttttcacg ggcgctcctg aaaaaatcac tgagcccaaa gcccgacagg
     3481 atcacgagga catgattgcg actgtccggg cgctgcggac agaaatgctg ctcccacagg
     3541 agagcacctt cgcgcgcatc cttgctcgca ctaaggagct ttcagcatca agtgccgaga
     3601 agctcatgca ggcctctgaa cgcgccaagg agcgagcaag gccgtatatt caggatgcca
     3661 aggaggttac ggaacgagcc atcgaggcgg ccaaacccac tctcgataaa gccaaagagt
     3721 atggtcgcaa gttctatgag caggcgcggg aagggctgaa gggcctcatg gattcgaact
     3781 ccgagacgga gccaccggct gcccagcccg aagagcctcg gaacgagccg cagcaataac
     3841 gcggccgatt aaatttgttg accggagcgg acgagctgat gaccaaacgc ctcgaaggcc
     3901 gcattgccct catcaccgga gcctcgcgcg gaatcggacg tgccgttgcc ctggcttttg
     3961 cacgcgaagg cgcgcacgtg gtgctgctcg cgagaactca aggggcgctt gaggagcttg
     4021 acgacgagat ccgcgagcaa ggc
//
LOCUS       DASUMG010000027         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_83633, whole genome shotgun sequence.
ACCESSION   DASUMG010000027 DASUMG010000000
VERSION     DASUMG010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83633"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VFX46_00465"
     CDS             complement(<1..487)
                     /locus_tag="VFX46_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599088.1"
                     /translation="MFNLSGKGALVTGATGGIGGAIARKLHAAGAAVAISGTKVDRLE
                     ALAAELGDRVHIVPCDLSDRAAVQKLAGEAEAKLGTLDILVNNAGLTRDNLFMRMKDE
                     EWDQVIAVNLTSTFMLCRAAVRNMMRRQWGRIINITSVSGIIGNPGQGNYAAAKAGMI
                     GM"
     gene            complement(491..1438)
                     /gene="fabD"
                     /locus_tag="VFX46_00470"
     CDS             complement(491..1438)
                     /gene="fabD"
                     /locus_tag="VFX46_00470"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5599089.1"
                     /translation="MAVAFVFPGQGSQDVGMGRELAETYKAARDVFDEVDDALGESLS
                     RIMWEGPKDVLTLTENAQPALMAVSMAAIRVLEQEKGFKLGEHVSYVAGHSLGEYSAL
                     AAAGALSIRDAARLLRARGRAMQQAVPVGQGAMVALLGVGLDTAEKVAAEAAQGEVCQ
                     VANDNEPTQVVLSGHKTAIDRVAEVGRKHGVRRAIPLPVSAPFHCSLMQPAADVMAAE
                     LEKVEVREPAVPVVANVLAEPISDPQEIKKRLVEQVTGTVRWRECVQKMGALGVTDFY
                     EIGAGKVLSGLVKRILKDANAMSVGTPADIDAALAALGR"
     gene            1854..>2278
                     /gene="rpsF"
                     /locus_tag="VFX46_00475"
     CDS             1854..>2278
                     /gene="rpsF"
                     /locus_tag="VFX46_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5599090.1"
                     /translation="MPLYEHVFLARQEVSPQQVEAMIKEFSQVITEGGGTVGKTEYWG
                     LKTLAYKIKKNRKAHYALMNIDAQPDVVAEMERRMRLSPDVLRFLTLRVEEHETEPSV
                     QMRKSDDRGGSKGGRSSAQAGASTFRAKPAKRDQAEAES"
BASE COUNT          423 a          743 c          678 g          434 t
ORIGIN      
        1 tcataccgat catgccggcc ttggcggcgg catagttccc ttgtcccggg ttgccgatga
       61 taccagacac cgacgtgata ttgatgattc ggccccattg tcggcgcatc atatttcgca
      121 cagccgcgcg gcagagcatg aatgtcgacg tcagattgac ggcgatgacc tggtcccact
      181 cttcatcctt catgcgcatg aagaggttat ctctggttag gccggcgttg ttcaccagga
      241 tatccagcgt gccaagcttg gcctcggcct cgccggccag cttctgaacc gccgcacgat
      301 cgctcaggtc gcacggcacg atgtgcacgc gatcgccgag ctcggcggcc agtgcttcca
      361 gacggtcaac ctttgtgccg gaaatggcaa ccgcggcccc tgccgcatgc agcttgcgcg
      421 cgatggcacc tccgatgccc ccggtcgcgc cggtgaccag cgcccctttg ccagacagat
      481 tgaacatggc ttatctcccg agcgccgcaa gtgcggcatc aatatcagca ggcgtgccga
      541 cggacatcgc attggcgtcc ttgaggatcc gcttcacgag cccagacagc accttgcccg
      601 ctccgatttc gtagaaatct gtcacgccga gggcacccat cttttggacg cactcgcgcc
      661 agcgcaccgt gccggtgacc tgctcgacaa ggcgcttctt gatttcctga ggatcgctga
      721 tgggctccgc caggacgttg gcaaccacgg ggacggccgg ctcgcgcacc tcgacctttt
      781 ccagctcggc ggccattaca tcggctgcag gttgcatcag cgaacaatgg aacggggcgc
      841 tcacaggtag tggaatcgcg cgccgcacgc catgcttacg gccgacctct gcgacccggt
      901 cgatcgcggt cttgtgcccc gaaaggacaa cttgagtcgg ctcattatca ttggcgacct
      961 ggcagacctc gccctgcgcc gcctcggctg cgaccttttc ggctgtatcg agaccgacac
     1021 cgagcagcgc caccatcgct ccctgcccaa cgggcacggc ctgttgcatt gcacggccac
     1081 gggcgcgcag gagacgcgct gcatcgcgaa tggaaagcgc tccggccgca gccagtgcgg
     1141 aatattcacc caaggagtgg ccggctacgt agctcacgtg ctcgccaagc ttgaacccct
     1201 tctcctgctc cagcacgcgg atcgcggcca tggagacggc catcagcgcc ggctgggcgt
     1261 tctctgtcag ggtgagaacg tccttcgggc cttcccacat gatccgggat agggattctc
     1321 ccaacgcgtc gtcgacttcg tcgaagacgt cccgggccgc cttgtacgtc tcggctagct
     1381 cccggcccat gcccacgtcc tggctgccct ggcccgggaa gacaaatgcc accgccatct
     1441 aagctgccgc ccttccctcg attcttcggc gcaaatagtg agtaggatcg agcgaataaa
     1501 gcaatcgtct cgtttcgccc acccgccaat tcgctacaac ttttcccggc gctttaggca
     1561 tggctcgcct acccctgtca agttggcggg agcaaaactc ttgcgatacg acacttttcg
     1621 cgtataagag ccgcgttcac gaagcgacgc catcccggtt gggggctgaa cggcgggcgt
     1681 tctttttcca cacagtgtgg aaaaaggggg tgcaagggca accgcccctg ccgccggtgt
     1741 ctccgctctt tgaggtcgtc gttgaggcct ttcaagccgc cattcgatgc ggctcgtccg
     1801 gcctcaatag gggctctgcg ccgggatgga caacaggatg aaaggctaga ttcatgccac
     1861 tctacgagca tgttttcctg gcgcgccagg aggtctcgcc gcagcaggtc gaggccatga
     1921 tcaaggaatt ctcgcaggtt atcacggaag gtggcggtac ggtcggcaag accgagtact
     1981 ggggcctgaa gaccttggcc tacaagatca agaagaaccg caaggcgcat tacgccctta
     2041 tgaacattga cgcgcagccg gacgtcgtcg cggaaatgga gcggcgcatg cgtctgtcgc
     2101 cggatgtcct gcgctttttg acgctgcgcg tcgaagaaca cgagaccgag ccctccgtgc
     2161 agatgcgcaa gagcgatgat cgtggcggca gcaagggcgg ccgttcatca gcgcaggccg
     2221 gcgcgtcgac gttccgcgcc aagcccgcca agcgtgatca ggcagaggcc gaatcgga
//
LOCUS       DASUMG010000028         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_3337, whole genome shotgun sequence.
ACCESSION   DASUMG010000028 DASUMG010000000
VERSION     DASUMG010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3337"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VFX46_00480"
     CDS             complement(<1..464)
                     /locus_tag="VFX46_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5599091.1"
                     /translation="MPDAITTSLIPIVIILLILASAIKILREYERGVVFTLGRFWGVK
                     GPGLIIIIPGIQQFVRVDLRTRVLDVPPQDVISRDNVSVRVNAVIYYRVIDAERAVIQ
                     VEEFEAATSQLAQTTLRSVVGQHDLDEMLAEREKINNKIQDILDAQTDAWGI"
     gene            complement(481..1950)
                     /locus_tag="VFX46_00485"
     CDS             complement(481..1950)
                     /locus_tag="VFX46_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5599092.1"
                     /translation="MRNRSARISFYRGTRREKMKADLRSGQSLQSFQSAEATCGRGVR
                     LALAALALLTGLALIFVHPTSGQQNRDGPVLVFDVKGVIGFTSVEHLQRSVERAKAEN
                     ASLIIMRIDTPGGLVSSTRDMIQAILASPIPIVGYVAPSGARAASAGTYLMYAAHLAA
                     MAPATHLGAATPIQMGPPGITPSPNEKKDKASEPDAAERKLINDAVSYLRGLAELRGR
                     NADWAEKAVREGATLTSTQAQEEKVIDFIASDLREVLEKANGRTVTTSTGTITLDTRD
                     RRIIEVEPSWHMQVLEVIANPNVAFILMLLGIYGIIFELLNPGALFPGVIGGICLILA
                     LTALTVLPVNLGGVALLLLGIGLLAAEAFTPSFGILGIGGIVAFILGGLFLFDPEEVD
                     IQFEVSRPLVIGAAIASAAILAGVVGMAVQSRKRTVQAGAEQLIGGTAEVVEWSGHEG
                     RVRVHGEIWLAESEMELASGDRVRVIGREGLKLTVKKTS"
     gene            2040..2987
                     /locus_tag="VFX46_00490"
     CDS             2040..2987
                     /locus_tag="VFX46_00490"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5599093.1"
                     /translation="MKIAIIGVGAMGSVYAALLASAGHEVWAIDQWKEHIDAIREKGL
                     RVEGASGDRTVRIAATTDPADVRDADLVIIATKDNGVVDAARSALQVAKEDAPILTIQ
                     NGLGSADKVAEIVGSKRVMLGVVGGFGASIRAPGHAHHNGMEFVRLGEMEGGLTPRLE
                     AVAEVWRSGGFRTLTFEDIHKMVWEKLICNVTYSGTCALTGLTIGEVQANPSAWSVAT
                     ACANEAYQVARAKGIALDFDDPIAYVRAFGEKIPHAKPSMLLDHLAGRPAEIDNINGA
                     IPREGAKVGIATPVNSLVVALLKAKESAFGAAKAPGQNA"
     gene            complement(2984..3466)
                     /gene="rlmH"
                     /locus_tag="VFX46_00495"
     CDS             complement(2984..3466)
                     /gene="rlmH"
                     /locus_tag="VFX46_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX5599094.1"
                     /translation="MRIIIAAVGRLKEGPERQLFERYRDRLAAISRGVGWGPFDVAEI
                     PESRASNADARKSDEAARLLAKVDAAGVRIALDENGREVTSEAFARYLQQQRQAGVSC
                     LAFLIGGPDGHGAEALARADLKLSLGKMTLPHMLVRVILVEQLYRAATILTGHPYHRG
                     "
     gene            complement(3527..3934)
                     /gene="rsfS"
                     /locus_tag="VFX46_00500"
     CDS             complement(3527..3934)
                     /gene="rsfS"
                     /locus_tag="VFX46_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX5599095.1"
                     /translation="MQSALEGARRGVPPAEFASEGRNSETLLAHVVHWLDEAKAEEVV
                     TIDLRNKSSIGDYMVIASGRSDRHVGAIADQIQRKLKEHGFGRARIEGAEACDWVLID
                     ALDVIVHVFRPEVREFYNLEKMWSAERPAESAH"
BASE COUNT          816 a         1325 c         1273 g          839 t
ORIGIN      
        1 ttgatacccc aggcgtccgt ctgggcatcg agaatgtcct ggatcttgtt attgatcttc
       61 tctcgctccg ccaacatctc gtcgaggtcg tgctgaccga caactgaacg caacgtcgtc
      121 tgggcgagct gactggtcgc tgcctcgaac tcctccactt ggattaccgc gcgctcggca
      181 tcgatcacgc ggtagtagat cacggcattc acccgaaccg aaacgttgtc gcgagaaatg
      241 acgtcctgtg gcggcacgtc gagcacgcgc gtcctcaaat cgacgcgcac gaattgctgg
      301 atgccgggaa taatgatgat gagccccggg cctttgacgc cccaaaaccg gccgagggtg
      361 aaaaccacac cgcgctcata ttcgcgcagg attttgatgg ccgatgccaa gatcagcagg
      421 ataattacta tcggaatgag actcgttgtt atcgcgtctg gcatggctct ctccctctcg
      481 tcagctcgtc tttttcaccg tcaatttcag cccttcacgc ccgattaccc ggacacgatc
      541 accggaggcc aactccattt cggactccgc taaccagatt tccccgtgca cccgcacgcg
      601 gccttcgtga cccgaccatt ccacaacctc cgcggtgccg ccgatgagct gctcggcacc
      661 ggcttggacc gtccgcttcc gtgactggac cgccattccc accacacccg cgagaattgc
      721 cgcgctcgcg atagcggcgc cgatcaccaa gggccgggag acctcgaact gaatatccac
      781 ctcttcggga tcgaacagga acaatcctcc gaggatgaat gccacgatgc cgccgattcc
      841 gaggatgccg aagcttggcg tgaacgcctc ggctgctaac agcccgattc caagaagcag
      901 caacgccacg ccgccgagat tgaccgggag aacagtcaaa gccgtcaggg caaggatcag
      961 gcagatgcca ccgatgacgc ccggaaacag cgcgcctgga ttcagcagct cgaaaatgat
     1021 gccgtagatg ccgagcagca taagaatgaa agcgacattc ggattggcga tcacctcgag
     1081 aacttgcatg tgccagcttg gctccacctc gatgatgcgc cgatcgcgcg tatcgagcgt
     1141 gatggtgcca gtgctcgtcg tgaccgtgcg tccgttggct ttttcgagca cctccctcag
     1201 gtccgaagcg atgaagtcga tgaccttctc ttcctgagct tgggtagaag tcaaagtcgc
     1261 gccctcgcgc acggcttttt cggcccaatc ggcattccga cctctgagct cggccagacc
     1321 gcggagatac gacaccgcgt cgttgatcag ttttcgctcg gctgcatctg gctcagaggc
     1381 cttgtccttc ttctcgttcg gggaaggcgt aatcccggga gggcccatct gaatcggtgt
     1441 ggcggcgccc aagtgcgtag cgggcgccat cgctgcgaga tgagccgcgt acatcaaata
     1501 ggtgcccgcg ctcgccgcac gcgcaccgct gggcgcgacg taaccgacaa ttggaattgg
     1561 cgatgcaagg atcgcttgga tcatgtcgcg ggtcgacgag accaggccgc ccggcgtgtc
     1621 gatgcgcatg atgatcaggc tggcgttttc ggccttggcg cgttcgacgc tcctctgaag
     1681 gtgctctact gacgtgaagc caatcacgcc cttgacgtcg aacaccaaaa cggggccgtc
     1741 gcgattttgc tgtccgctgg tcgggtggac gaatatgagc gcgaggcctg tcagcagggc
     1801 cagagcggcg agggcaagcc tgacgccacg gccacaagtc gcctctgccg attgaaagct
     1861 ctgcaatgac tgccctgatc tgaggtccgc tttcatcttt tcccgccggg ttccacggta
     1921 gaaactgatg cgggcgctac ggttccgcat cggcagccct cccgaccaac gccacgcgcc
     1981 cattcgatgg tcgaccccaa gccgggcctg taggatcgca accttggagg aaagatacca
     2041 tgaaaatcgc gattatcggc gtcggagcga tgggctccgt gtatgcggca cttctggcct
     2101 ccgcgggcca cgaggtgtgg gccatcgatc agtggaagga gcacatcgat gctatccggg
     2161 agaaaggcct tcgggtggag ggggcttcag gcgaccgcac cgtcaggata gcggccacga
     2221 ccgacccggc ggatgtccgc gacgctgacc tcgttatcat cgccaccaag gataacggcg
     2281 tcgtcgatgc ggcccgttcg gctctccagg tggcgaaaga ggatgctcca attctgacga
     2341 ttcagaatgg gctcggcagc gccgacaaag tggccgaaat cgttggatcc aaacgggtca
     2401 tgttgggtgt cgtcggcggc ttcggtgcct ccattcgcgc ccccggtcat gcccaccaca
     2461 atggcatgga gttcgtgcgc ctcggtgaga tggagggagg cctcaccccg cgcttggagg
     2521 ccgtggccga ggtttggcgc tccggcggct ttcgaacgct cacgttcgag gacatccaca
     2581 aaatggtctg ggagaagctg atctgtaacg tcacctattc aggcacctgc gccttgaccg
     2641 gccttacaat cggagaggtt caggctaatc ccagcgcgtg gagcgtcgcg accgcctgcg
     2701 cgaacgaggc ttatcaggtg gcgcgggcga aagggatcgc gctcgacttc gatgatccca
     2761 tcgcttacgt tcgcgccttt ggcgagaaaa tacctcatgc gaagccttct atgcttctcg
     2821 accatctcgc cggccgtccc gcggagatcg acaacatcaa cggcgcaatt ccgcgcgagg
     2881 gcgccaaggt cggtattgcg acgccggtga actctctcgt cgtggccctg ctgaaagcaa
     2941 aggaatcggc atttggagct gcgaaggccc caggccagaa tgcttagccg cgatgatagg
     3001 gatggccggt cagaatcgtc gcggcgcgat agagctgctc gaccagaatc acgcggacga
     3061 gcatgtgggg gagtgtcatt tttccgagtg agagcttgag atcggcgcga gccagcgcct
     3121 cggcgccgtg gccgtcggga cccccgatga gaaaggcaag gcaagaaacg cccgcctgcc
     3181 gctgctgctg caggtatctg gcgaaggctt cgctggtgac ctcgcgacca ttctcgtcga
     3241 gggcgatcct gactccagcg gcatcaactt tggcgagcaa tcgcgccgcc tcgtcagact
     3301 tccgcgcatc cgcgtttgat gcgcggctct cagggatttc ggctacgtcg aaggggcccc
     3361 agccgacacc acggctgatc gctgccagcc gatcgcgata gcgctcgaaa agctggcgct
     3421 caggcccctc tttgaggcgt ccgactgctg cgatgatgat gcgcatgggc cgcttccctc
     3481 atcgcgggac cggtccgcaa cgatcaggtc cgcagctgtc cggccctcaa tgggcgcttt
     3541 ccgccgggcg ctctgccgac cacatcttct ccagattgta gaactcgcgc acctctggac
     3601 ggaagacgtg gacgatgacg tccagcgcgt cgatcagcac ccaatcgcac gcctccgcac
     3661 cctctatgcg ggcgcgtccg aaaccatgtt ccttcagctt gcgctggatc tgatccgcga
     3721 tggctccgac atgacggtca gagcggccac ttgcgatcac catgtagtca ccgatcgatg
     3781 atttgttccg gagatcgatg gtgaccacct cctcggcctt ggcctcatcg agccagtgga
     3841 cgacgtgggc gaggagcgtt tccgagttcc ttccttctga ggcgaactcg gccggaggca
     3901 cgccccggcg ggcgccctca agagcagatt gcatcgtgtg gtggatttcc tttcgccatc
     3961 aagtcccaat ttgggtgctg ttctccgcgt tgttcaccat tcgggtggcg gcaacagcca
     4021 acaaagtcac cattcgggtg accgtgccaa gaataagtgg tttcgatact gattttcaac
     4081 ccgaattgat cgcggaattc ccatttcggg gcacggcgtt gccgtccggt ccggctagac
     4141 agggctgcac atctgtcgga attaagctta agcctcttct tccttacggc tttcgcgtga
     4201 gagtgccgag accaggcggt gccttgcctc aggcatggtc atgtcccgcg gaa
//
LOCUS       DASUMG010000029         5674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_967433, whole genome shotgun sequence.
ACCESSION   DASUMG010000029 DASUMG010000000
VERSION     DASUMG010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967433"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1077)
                     /locus_tag="VFX46_00505"
     CDS             complement(52..1077)
                     /locus_tag="VFX46_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599096.1"
                     /translation="MTTIHAAAASRLLRRLAFAALASIFAAGLTACSSDSNILGTSGD
                     NSQLLSQTAVQPSPAPTQISIAPVIGAPEAVGNQLSAQLAQGLEQKSVSVAKSPSDRA
                     DFTLRGYIVAAKEKNGTKVSYIWDVTDPTGKRVHRITGEELASKTDSRDPWTAVTPDV
                     MQSITGKTATAIATWMQSEAAVANTSSANEPVRTAALSSSGAAANPTPAAAPAATASI
                     SGSGPITAMVPSVTGAPGDGSVSLTSALQRELARSGIALADRPTGNTYRVEGRVKLGQ
                     SQNGKQPIQIDWHVKDPQGKQLGTVSQKNEIPEGSLDGEWGKVADAAASAAAQGILKL
                     LPNRSTN"
     gene            complement(1274..2056)
                     /gene="pgeF"
                     /locus_tag="VFX46_00510"
     CDS             complement(1274..2056)
                     /gene="pgeF"
                     /locus_tag="VFX46_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEX5599097.1"
                     /translation="MAVDGTIIRADCLSDIAGVSHGFFTRQGGVSTGLYDSLNCGLGS
                     KDDPNLVRQNRARVARTLGAPRDEVLTLYQIHSAKALVVDRPFDPQQIPRADALVTRT
                     PGLVIGALAADCTPVLFADPIARVIAASHAGWRGALAGILESTIETMIGLGARRDRIR
                     AAIGPCINQAAYEVGPEFEAEFLAADRNNARFFVRPAPDARPRFDLPGYVEHRLIQTG
                     LAVVERRSLCSYENESLFFSYRRATLRKEPDYGRQISAIVLT"
     gene            complement(2070..3179)
                     /locus_tag="VFX46_00515"
     CDS             complement(2070..3179)
                     /locus_tag="VFX46_00515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5599098.1"
                     /translation="MTSEQQPTPLALKLKERIRREGPLPVLEYMRACLNDPDHGYYRG
                     QPAIGASGDFTTAPEISQIFGELIGIWAAVAWRAMGAPPQVDLIELGPGRGTMMSDAL
                     RAARVVPGFLEAIQLRLVESNPFLRETQRQTLAPALQHVPVSWHDEFTPAIAGIRPTI
                     ILANEFLDALPVRQLVFTAGEWRERCVGLDSHECFELVPGAPAHPPRLPAGRAPQEGD
                     VLETHPDFAPIADVLRQWSTQPLAALFIDYGHTETAFGETLQAVSRHRYASIFEKPGE
                     TDLTAHVDFAEFKDLCVERGLAVDGPITQSEFLFGMGLAERAGKLMSAARPSEAGLIE
                     ASVQRIADPLGMGGRFKVVCVRSGVPVLPPFPPGG"
     gene            complement(3187..4053)
                     /gene="lgt"
                     /locus_tag="VFX46_00520"
     CDS             complement(3187..4053)
                     /gene="lgt"
                     /locus_tag="VFX46_00520"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014765333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX5599099.1"
                     /translation="MSSFASFLALPFPNIDPVAFQLGPVTVKWYGLAYMAGLVLGWLY
                     IRRLLRNPALWSGGKPPFGVEKTDDLLFFMTLGVVLGGRLGFVLFYEPLYYLNHPAEI
                     PQVWRGGMAFHGALVGCGIAIWAFARRAGVSPLSSMDMCAAAVPIGLFFGRIANFING
                     ELFGRPTEVPWGMVFPEAMYQYAAIEPTPRHPSQLYEAFLEGLVLFLVLRWMTHQAHA
                     LKSPGLVAGTFMVGYGVARSFCEFFRQPDPLHPLTIGFLTPGIAYSIPMILAGLYFIR
                     AARARSSPVSQP"
     gene            4231..4497
                     /locus_tag="VFX46_00525"
     CDS             4231..4497
                     /locus_tag="VFX46_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX5599100.1"
                     /translation="MTQTTNRFFDELAKMMTDAAGAAQGLRREFESFMKAQGERILRE
                     MDLVRRDEFDAVKAMAQRAREENEKLEARIAALEAELAALKGKA"
     gene            4819..5316
                     /locus_tag="VFX46_00530"
     CDS             4819..5316
                     /locus_tag="VFX46_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5599101.1"
                     /translation="MASAEAGYQRMSNPIDIVEQIAHNNGWACDRTADDEVTLIVAGS
                     WTDYHLSLNWREDLEVLHLACAFDFKVPDNRLTEIYKLIAQINEQLWLGHFDLWTQEG
                     LIMFRHGLMLNGALATPPQVEALLKAALEACERYYQAFQFVVWAGKESREALVSTMFH
                     TEGQA"
     gene            5485..>5674
                     /locus_tag="VFX46_00535"
     CDS             5485..>5674
                     /locus_tag="VFX46_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5599102.1"
                     /translation="MELDAVSFSLKGKLLLAGAGKMGGAMLAGWLNRGLAPSQIIVQD
                     PAPPLESAELISRHGIEVR"
BASE COUNT         1136 a         1692 c         1807 g         1039 t
ORIGIN      
        1 gtaaacgtcc gggccttgct tttcatgacg gcaatatgaa gcggatggag cctagttcgt
       61 gctcctgttt ggcagcagct tcaggatgcc ctgagcggcg gcggaggcag ctgcatcagc
      121 caccttgccc cattcgccgt cgagcgagcc ttctggaatc tcgttcttct gcgagactgt
      181 gccaagctgc ttgccctgcg gatccttgac gtgccagtcg atctggatcg gttgtttgcc
      241 gttctggctc tgacccagct tcacgcgacc ttcgaccctg taggtatttc cggtcgggcg
      301 atcagccaaa gcaatgccgc tgcgggcaag ctcacgttgc agcgcgctcg tcagcgaaac
      361 cgagccgtca cccggagcac ctgtgacact cggcaccatg gcagtaatgg ggccgctgcc
      421 gctgatgctg gccgtcgcag ccggcgccgc cgctggcgtc gggtttgccg ctgccccgga
      481 ggacgagagc gccgcggtcc tgaccggctc attcgccgag ctggtgttcg ccaccgcagc
      541 ctcggactgc atccaggttg cgattgcagt tgccgtcttg cccgtgatcg attgcatcac
      601 gtctggcgtc acggcagtcc agggatcacg cgagtccgtc ttgcttgcca gctcctcgcc
      661 ggtgatgcgg tgaacccgct tgcccgtcgg gtctgtcaca tcccagatgt acgacacctt
      721 ggtgccgttc ttctccttcg cggcgacgat gtagccgcgc agcgtgaagt cggcacgatc
      781 cgacggcgac ttggcaaccg atacgctctt ctgctcaagg ccctgagcga gttgggctga
      841 gagctgattg cctaccgcct caggcgcacc gatgaccggc gcgatggaga tctgtgttgg
      901 cgcgggggat ggctgtacag cggtctgaga taacagctgc gagttgtccc ccgaagtgcc
      961 cagaatgttt gaatcactgc tgcaggcagt gaggcctgca gcgaaaatcg atgccagcgc
     1021 tgcgaacgca agcctcctca aaaggcgcga cgccgcagca gcatggatgg tcgtcacgat
     1081 gacgtccccc ttttatgtcc cgctgttcaa ccggcagccg acgtaccaac tctgcggcct
     1141 ctgagagaat ccggtacaca cacgcgaatc ctgtaactct tgcgtcgaga ggcgtccaga
     1201 tcgtttgccc tcagcaggcc gtatagtgcc ccatagccgc ctgaacgagc cggtgttttc
     1261 tgtggatgaa ggcctaggtc aagacaatgg cggagatttg gcggccgtaa tccggctctt
     1321 tcctaagcgt tgcgcggcga tagctgaaga aaagtgattc gttttcataa ctgcacaggg
     1381 accgccgttc gaccaccgcc aaccctgttt gaatcagccg gtgctcgaca tagcccggga
     1441 gatcaaatct cggacgcgca tcgggcgcag gccggacgaa gaatcgagcg ttgttcctat
     1501 cggcggcaag gaattcggct tcgaattccg ggccgacctc ataagcagct tggttaatgc
     1561 aggggccaat agcagcgcga atccggtcgc ggcgggcgcc gaggccgatc atggtctcta
     1621 tggttgattc gagaatgcca gccagcgcgc cgcgccagcc ggcgtgagag gcggcaatca
     1681 ctcgcgcaat cggatcggcg aaaagaaccg gggtacagtc cgccgccaga gcaccgatca
     1741 cgagaccagg cgtgcgcgta accagcgcat cggcgcgggg aatctgctgc ggatcgaaag
     1801 gccggtcgac gacgagcgct ttcgcgctgt ggatctgata cagggtcaga acctcgtcgc
     1861 gaggagcgcc caatgttcgg gcgacgcgcg cccgattctg gcggacgaga ttcggatcgt
     1921 ccttggaccc caagccgcaa ttcaggctgt cgtagaggcc agtggaaacg ccaccctgcc
     1981 gcgtgaaaaa gccatggctg acacctgcga tgtcgcttag gcagtcggcg cgaatgatcg
     2041 ttccgtctac agccatttgt attccctttt cacccacctg gtgggaaagg cggcaacaca
     2101 gggacgccgg agcgcacgca cacgaccttg aatcggccac ccatgccgag tggatcagca
     2161 atgcgctgca cgcttgcctc tatcaaaccg gcttcgctgg gtcgagctgc ggacatgagc
     2221 ttgcctgcgc gttcagccaa ccccatgccg aataggaatt cggactgtgt gattgggccg
     2281 tccacagcca gtccgcgttc gacgcacaaa tccttgaact cggcaaaatc gacgtgggcg
     2341 gtcaaatccg tctcgccagg tttttcgaaa atcgaagcgt accggtggcg gctgaccgcc
     2401 tgcaaggttt caccaaatgc cgtttccgtg tggccgtagt cgatgaaaag ggccgcaagc
     2461 ggctgtgtcg accattggcg caacacatcg gcgattggtg cgaaatccgg gtgagtttcg
     2521 agaacatcgc cttcctgcgg cgcacggccg gcaggaagcc gcggaggatg agccggagca
     2581 ccgggcacca actcgaagca ttcgtgcgaa tcgagcccga cacagcgctc gcgccattca
     2641 ccggcggtga acacaagttg gcgtacggga agagcgtcga gaaactcgtt cgccaggatg
     2701 attgtcggcc ggattccggc aatggcaggc gtaaactcgt cgtgccatga cacgggcacg
     2761 tgttggagcg ccggggcaag cgtctggcgc tgagtttccc ggagaaaggg attgctctcg
     2821 accagacgga gctggatggc ttccaggaaa ccgggaacga cccgagccgc ccgcaacgca
     2881 tcgctcatca tcgttccacg ccccggacca agctcgatca ggtcaacttg cggcggcgcg
     2941 cccatcgcgc gccaggccac ggcggcccat atcccaatca actcgccgaa gatctgactg
     3001 atctccggtg ccgtcgtgaa atcgccgctt gcgccaatgg cgggttgacc acggtagtac
     3061 ccatgatccg ggtcattgag acaggcgcgc atgtactcca gaacgggcag cggcccctcg
     3121 cgcctgatcc gctccttgag cttcaaggcg agaggtgtcg gctgctgctc gctcgtcatc
     3181 gcgctcctac ggctggctta caggggaaga gcgggcccgc gcggcgcgga tgaagtacag
     3241 gccagccagg atcatcggga tcgagtaggc aatgccaggg gtcaggaatc caatggtcaa
     3301 cggatgcaat gggtcgggct ggcggaagaa ctcacagaag gagcgtgcca cgccgtagcc
     3361 aaccatgaac gtgcccgcga cgaggccagg gctcttgagc gcgtgggctt ggtgggtcat
     3421 ccagcggagc accaggaaca acaccagccc ctcgagaaat gcctcataaa gctggctggg
     3481 atggcgcggc gtcggctcga tagcggcgta ctggtacatg gcctcgggga acaccatacc
     3541 ccatggcacc tctgtcggac ggccgaaaag ctcaccgtta atgaaattcg cgatgcggcc
     3601 gaagaagagg ccgatcggta cggcggccgc acacatgtcc atggaggaca agggcgagac
     3661 accggcgcgc cgggcaaagg cccaaatggc gattccgcag ccgaccagcg ccccgtggaa
     3721 cgccatgcca ccacgccaga cctgcggaat ttcggccgga tggttcaggt aatagagcgg
     3781 ttcgtagaag aggacgaatc cgagacggcc gcccaaaacc acgcccagcg tcatgaagaa
     3841 cagcaggtcg tccgtcttct ccacgccgaa aggtggcttg cctcccgacc acagagcagg
     3901 gtttcgcagc aaccgccgga tatagagcca gccgaggacg agccccgcca tataggcgag
     3961 gccgtaccac ttcaccgtga ccgggccgag ctggaacgcg accggatcga tgtttggaaa
     4021 cgggagggcg agaaagctcg cgaaagaact catcgacagc ctgtgtggaa gcggaagttt
     4081 agcggctcgc cagcaacatt gctgcgcgcc ggacgttgac gggtcggtgc ggctaatgtc
     4141 aaccgggtgc gccgatatgc ttgcgcggga gcgcggcggc acccatagtg actgacatgt
     4201 ctcccataga tagcgactgg cgctaatcca atgacccaga ccaccaatcg ctttttcgac
     4261 gagttggcca agatgatgac ggatgcggca ggcgccgccc agggtctgcg ccgcgagttc
     4321 gagtcgttca tgaaagccca gggcgagcgt atcttacgcg agatggatct cgtccggcgc
     4381 gatgagttcg atgccgtcaa ggcgatggcc cagcgcgccc gggaggagaa tgaaaagctc
     4441 gaggctcgaa ttgcggctct ggaagccgaa ttggccgctc tcaagggcaa agcttaagcc
     4501 tttccgggcc gccgtgagtc gcttgctgtt tcggcgctga cactgctcgc aacatcccca
     4561 ggttttgcat cagtcgcgca cacgtcctcc aaaagcggcg caaaatggct tgcatcgccc
     4621 gaaactcatc actttggccg tggacaatgg gagggtgagc ttgcgactcg tcccttgcat
     4681 ctgttttggt cgctcccgct cagcagccgg ttgatccggt tcatcacagc gtccggcgct
     4741 gatcgtcgtg aggggatcag gggatgtgag cgtagatcct caaacatcgc ggcggaacaa
     4801 caagggggat ggctgggaat ggcatcggcg gaagcaggct atcagcgcat gagcaatcca
     4861 atcgatatcg tcgaacagat agcccataac aacggctggg cgtgtgaccg cactgcggat
     4921 gacgaagtta cgctgatcgt tgcggggagc tggacggatt atcacctctc gctcaactgg
     4981 cgcgaggatc tcgaggtgct gcatctggct tgcgccttcg atttcaaggt cccggacaac
     5041 cgtctgaccg aaatttacaa gctgatagcg cagatcaacg agcagctctg gctcggccat
     5101 ttcgatcttt ggacccagga aggactgatc atgttccgcc atgggctgat gctcaacggc
     5161 gcgctggcga cgccgccgca ggtggaggcc ttgttgaagg cagcccttga ggcctgcgag
     5221 cggtactatc aggccttcca gttcgtcgtc tgggctggaa aggaaagccg ggaggccctc
     5281 gtctccacga tgtttcacac cgaggggcaa gcttagggcc tgctcagacg gcaaactata
     5341 cgttaacttg cccaaaggca gcagcatcgg cggctggtga cgagcgccag ggtggcgcgt
     5401 tattgggata cggcctacag aatggattgg gcgggattag gctcgcatcc atgagagact
     5461 gcggctgttg gtgtctttaa tcgagtggag ctcgacgcgg tgtcgttcag cctgaagggc
     5521 aagttgttgc tggccggcgc gggcaagatg ggcggggcca tgctggccgg gtggctcaat
     5581 cgcggcttgg cgccgtcgca gatcatcgtg caggatcctg cgccgccgct cgaaagcgcc
     5641 gagctcattt cgcggcatgg catcgaggtc cggc
//
LOCUS       DASUMG010000030         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_967446, whole genome shotgun sequence.
ACCESSION   DASUMG010000030 DASUMG010000000
VERSION     DASUMG010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967446"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..520
                     /locus_tag="VFX46_00540"
     CDS             149..520
                     /locus_tag="VFX46_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5599103.1"
                     /translation="MTDTTVKKVHSADSPKGEMGQTYLVSGKRVSMRLWQGEPADRHK
                     RESVRDYETVGYVIDGRAELELEGQTIRLEPGDSWLVPAGAKHTYRILEPFTAVEATA
                     PPAQVHGRDEPRSAMDWPERL"
     gene            801..1130
                     /locus_tag="VFX46_00545"
     CDS             801..1130
                     /locus_tag="VFX46_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5599104.1"
                     /translation="MTQTPFDIAEFETAAAQVAETLRALSNERRLMILCKLVEWGEAN
                     VTSLGEAVGLSQSALSQHLAVMREQGIVTFRRQGQTLWYRIADPRIERLLATLHELYC
                     PSPGKHV"
     gene            1190..1720
                     /locus_tag="VFX46_00550"
     CDS             1190..1720
                     /locus_tag="VFX46_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HEX5599105.1"
                     /translation="MSLIPIKPEDAQRYIAEGAVLIDIREDDEHARECIPGARHAPMS
                     RFGPIDTGDATAVIFHCRTGGRTTAASEKLAQAVKVKAYVLEGGLDAWKKAGLPVVKD
                     KSQPIELQRQVQIAAGSLVLIGIALGSLVSPTFYALSAFVGAGLVFAGVSGFCGMARI
                     LAYMPWNRRSHQPATA"
     gene            1849..>2264
                     /locus_tag="VFX46_00555"
     CDS             1849..>2264
                     /locus_tag="VFX46_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5599106.1"
                     /translation="MFKHILVTTDGSEFAERAFSHAIELAKHLGAKLTAVTVTPPIQL
                     LTIEGVVYPEDPEKFQAMTDAQAKAALDAVVKAAEAQGVSCDCVQIENPQPYEGIIQT
                     AKDRGCDLIVMASHGRRGLSAFLLGSETQKVLTHTTV"
BASE COUNT          449 a          684 c          706 g          425 t
ORIGIN      
        1 atcgagttca tccgcggcaa gtccgggtaa caggggttgg cgtctgtcat ttggaacggg
       61 agcatagtcc cgttggtttc cggaagagac gctcgtgcaa tgctatttcc gttccggcgt
      121 ggcacgggcc tgaaccggaa ctcgtacgat gacggacacg acggtgaaaa aagtccactc
      181 cgccgactcg cccaagggcg agatgggaca gacgtatctc gtctccggca aacgcgtatc
      241 gatgcggcta tggcagggcg agcccgccga taggcacaag cgggagagtg tgcgcgacta
      301 cgagacggtc ggctatgtca tcgatggccg agccgagctg gagctcgaag ggcagacaat
      361 tcggctggag cctggcgact cctggctggt tccggctggc gcgaagcaca cctacagaat
      421 cctcgagccc ttcactgcgg tcgaggcgac cgcgcctccc gctcaagtcc acggccgcga
      481 cgagccgcgc agcgcgatgg actggcctga acggctgtag tcgaattacg cggagccatc
      541 gcaaagcgcc gaagctccgg tggggactcg cgtcgcgctg gcacgctgag ccctgaactc
      601 gacttcgcac cacaatagca cagcacgaca gatctgctgg ttgcggagtt catctccagc
      661 aacgggagcc atcgttcatc ccggcggcga actcctttgc cttgctggag agcgtgtccg
      721 cctgtgctcg gccgcggccc ccatcaccat tgacaacgct ttagcgctac ctaatttagc
      781 attatctaat ggacgctgta atgacacaaa cccccttcga catcgcggaa ttcgagacag
      841 cggcggccca agtcgctgag accctgcgtg cgctctccaa tgagcgccgg ttgatgatct
      901 tgtgcaagct cgtcgagtgg ggcgaggcga acgtgacaag cttaggcgag gccgtcggcc
      961 tgtcgcagtc cgcactgtca cagcacctgg ccgtgatgcg cgagcagggc attgtcacct
     1021 ttcgccggca gggccaaacg ctttggtacc gcatcgctga tccgcgcatc gagcgtctgc
     1081 tcgcgactct tcacgagctc tactgcccat ccccgggaaa acacgtgtga cacatagcag
     1141 aagcgctcgg caaacgagtg cgcacggcag agatgagaag gagcccacga tgagcctcat
     1201 tccgatcaag cctgaggatg cccagcgtta catcgctgag ggtgcggtcc tcatcgacat
     1261 ccgggaggat gacgagcacg cgcgtgagtg catccccgga gcgcggcacg ctccgatgtc
     1321 ccgtttcggg ccgatcgata ccggtgatgc gactgcggtg attttccatt gccgtaccgg
     1381 tggccgcacc acagcggcca gcgagaagct tgcgcaggct gtcaaggtca aggcctatgt
     1441 tcttgaaggc ggtctcgatg cctggaagaa ggcagggctg cctgtcgtca aggataagag
     1501 ccagccgatc gagcttcagc gtcaggtgca gattgcggcc ggaagccttg tgctaattgg
     1561 cattgcgctc ggctcgcttg tttcgcctac attctatgca ctttcggcct tcgtcggtgc
     1621 tggtctcgtg ttcgccggtg tcagcggctt ctgcggcatg gcaaggatct tggcctacat
     1681 gccctggaac cggcgctcgc accagccggc aacggcctaa cccatcagcg attgctgcgc
     1741 ccgatcgcca ggtgatgcgt tgatgaagcc gggttgatgc gcgtcaaggc aaccccggca
     1801 gcgccagttc agttggtggc gtcaccagcg ctcagggagg agcagcagat gttcaagcat
     1861 atccttgtga ctaccgatgg gtcagagttt gcagagcggg ccttcagtca cgccatcgag
     1921 ctcgccaagc acctgggcgc caagctgacg gctgtgacgg tgacgccacc gattcagctg
     1981 ttgacgatcg aaggcgtcgt ttatccggag gacccggaaa agtttcaggc aatgaccgac
     2041 gcccaagcca aggcggcgtt ggacgctgtc gtcaaagccg ccgaagcgca gggcgtgtcc
     2101 tgcgattgcg tgcaaatcga gaaccctcag ccttacgagg gcatcattca aacggctaag
     2161 gacaggggct gcgatttgat cgtcatggcc tctcacggcc gtcgcggact atcggctttc
     2221 ctccttggca gcgaaacgca gaaagttctg acacacacga ccgt
//
LOCUS       DASUMG010000031         2896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_3641, whole genome shotgun sequence.
ACCESSION   DASUMG010000031 DASUMG010000000
VERSION     DASUMG010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3641"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..588)
                     /gene="rimM"
                     /locus_tag="VFX46_00560"
     CDS             complement(64..588)
                     /gene="rimM"
                     /locus_tag="VFX46_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734828.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5599107.1"
                     /translation="MGAAPARILLGRIRAAHGIRGHVLIHSFTEAPEDIASYGPLQDE
                     SGQRQFEITVVRVTPKGVVARISGIEDRTAAEALRGTQLYIERDRLPPTKEEEYYHSD
                     LIGLSAFKPNGERMGEVIAVHNFGAGDILEIRLEGKRTTEMVPFMRDFVPEVDVAGGR
                     LVVDMPEESEGGQS"
     gene            complement(619..972)
                     /gene="rpsP"
                     /locus_tag="VFX46_00565"
     CDS             complement(619..972)
                     /gene="rpsP"
                     /locus_tag="VFX46_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5599108.1"
                     /translation="MAVKIRLARGGAKKRPYYYIVVANSSAPRDGRYIEQVGTYNPLL
                     KKDDPNRVKLDIERCKHWLSVGAQPTDRVARFFDAAGLIKRQPKNNPIKGRPRKKRLE
                     REAAEAEKAKEAAGA"
     gene            complement(1237..2754)
                     /gene="ffh"
                     /locus_tag="VFX46_00570"
     CDS             complement(1237..2754)
                     /gene="ffh"
                     /locus_tag="VFX46_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5599109.1"
                     /translation="MFQSLSDRLSGIFDKLTKRGALTESDVSEAMREVRRALLEADVA
                     LEVVRDFTEKVKQKAIGQEVIKSVTPGQMVVKIVHDELVRMLGSDSQPIDVAATPPVP
                     ILMVGLQGSGKTTTTAKIAYRLKNRDKKRVLMASLDTRRPAAQEQLRVLGEQIDVPTL
                     PIIAGQTPLQIARRALDAARLGGYDVVLLDTAGRTTVDEALMLEVAEVKAATNPHEVL
                     LVADSLTGQDAVNTAKAFDNRVGITGIVLTRIDGDGRGGAALSMRAVTGKPIKLLGTG
                     EKWDALEDFHPERIANRILGMGDIVSLVEKAAQTIDAEKAQKIAQKMKKGEFDLEDLA
                     EQIKQMQKIGGMSGVLAMLPGIGKLRNQIAEANLDDKILKRQHAIILSMTPQERRNPK
                     IIDGKRKRRIAAGSGTKVEEVNRLLKMHRQMADMMKAMGKNKGLFARMFGGGGMPQPT
                     PEMVEQLQRGELPKGLPGLPPGGLPRLPGLGGGLPGLGGSGPKLPGLPGFPGKKK"
BASE COUNT          500 a          960 c          858 g          578 t
ORIGIN      
        1 ggcggccctc taatcgagat ttatcaccct gctcagctcg agattttgcc ctgaactcag
       61 cgctcagctc tgaccgcctt cactctcctc cggcatatcg acaacgagcc ggccccctgc
      121 cacatccacc tccggaacga aatcacgcat gaaggggacc atctccgtcg tgcgtttgcc
      181 ctcgagccgg atttccagaa tgtccccggc accgaaatta tggacggcga tcacctcgcc
      241 catccgttcg ccatttggct tgaaggcgct gagcccaata agatccgagt ggtaatattc
      301 ctcctccttg gttggaggga gccgatcgcg ctcgatatag agctgagtgc cgcgcaacgc
      361 ctcagcggca gtgcgatcct caatgccaga gatgcgcgcg accacacctt tcggcgtgac
      421 gcgaaccacc gtgatctcga actgccgttg tccagactca tcctgaagcg gaccgtagga
      481 tgcaatgtcc tccggcgctt cggtgaagct gtgaataagc acgtgccccc gaatgccgtg
      541 cgcggcgcgg attcgaccaa gcaggatccg cgcaggagca gcgcccattc accagctcct
      601 tgagggagtt ctgcggcgtt acgcgccagc ggcttccttc gccttctcgg cctcggcagc
      661 ttcgcgctcg agacgcttct tgcgcggcct acccttaatg ggattgttct tcggctgccg
      721 cttgatgagg cccgccgcat caaaaaaacg cgcgacgcga tccgtgggct gagcgccgac
      781 cgagagccag tgcttgcagc gctcgatatc gagcttcacc cgattgggat cgtccttttt
      841 cagaagagga ttgtaggtgc caacctgctc gatgtaccgg ccatcgcgcg gcgccgagct
      901 gttggcgacc acgatgtaat agtacgggcg cttctttgcg ccaccacgag caaggcggat
      961 ctttacagcc attattcgtc ttccttctct ttcatccgtg ttggacacat tcgcataagc
     1021 gaacgcggag ccaataggtt tgcgggtaag cacgccgagc cattgcgaaa atgctcagca
     1081 gccgttcaaa gcacgatgcc atcagagcct ctgcgctcgg gattaagcgg tctgcatgcg
     1141 tgagcacaac agatgcgaat cccggcgaca aatgccgcga tgacagttcg caagaaggct
     1201 cggggctacg gcagatcatg gtggtgtgac ccgaacctac ttcttctttc ccggaaaacc
     1261 gggaagccct ggaagcttgg gacctgaccc gcccaacccc ggcaagccgc cgccgaggcc
     1321 aggcaggcgc gggaggccgc caggcggcag gcccggcaaa cctttcggca actcgccgcg
     1381 ctgcagctgc tcgaccatct cgggcgtcgg ctgaggcata ccgccaccgc cgaacatgcg
     1441 cgcgaacagg cccttgttct tgcccatggc cttcatcatg tcggccatct ggcggtgcat
     1501 cttcagcagg cggttgacct cctcaacctt ggtgccggag ccggccgcaa tccgccgctt
     1561 acgcttgccg tcgatgatct tcgggttgcg gcgctcttgc ggcgtcatgg acaagatgat
     1621 cgcgtgctgc cgcttcaaaa tcttgtcgtc gagattggcc tcggcgatct gattgcggag
     1681 cttgccgatg ccgggcagca tcgcgagcac gccagacatg ccgccgatct tctgcatctg
     1741 cttgatctgc tcggcgaggt cctccaggtc gaactcgccc ttcttcatct tctgggcgat
     1801 cttctgcgcc ttttcggcgt cgatcgtctg cgcggccttc tcgaccaggc taacgatatc
     1861 gcccatcccc aggatgcggt tggcgatccg ctccgggtgg aagtcctcaa gcgcgtccca
     1921 cttctcgccg gtgccgagca gcttgatcgg cttgccggtg accgcacgca tggagagcgc
     1981 cgcaccgccg cggccatcgc cgtcaatgcg ggtcagcacg atgccggtga tgccgacacg
     2041 gttatcgaag gccttggccg tgttgacggc gtcctgaccc gtcaggctgt ccgcaacgag
     2101 cagcacttcg tgcgggttgg tggcggcctt gacctcggcc acctccagca tcagcgcctc
     2161 gtccacggtc gtgcggcccg cggtgtcgag cagcacaaca tcatatccgc cgaggcgggc
     2221 ggcatcgagc gcgcgccgcg caatctgcag tggcgtttgg cccgcgatga tcggcagcgt
     2281 cggcacgtcg atctgctcgc cgagcacgcg cagctgctcc tgcgccgccg gccggcgcgt
     2341 gtcgagcgag gccattagca cccgcttctt gtcgcggttc ttgaggcggt acgcgatctt
     2401 ggccgtagtg gtcgtcttac cggagccctg cagaccgacc atcaggatcg gcacgggcgg
     2461 cgtcgcggcg acatcaatgg gctgcgagtc cgagcccagc atgcgcacga gctcatcgtg
     2521 cacgatcttt acgaccatct ggccgggcgt gaccgacttg atcacctcct ggccgatcgc
     2581 cttctgcttc accttctcgg tgaagtcacg cacgacctcc agggccacgt ccgcctcgag
     2641 aagagcacgg cgcacctcgc gcatggcctc gctgacgtcg ctttcggtca gcgcgccgcg
     2701 cttggtgagc ttgtcgaata tgcctgagag gcggtcggaa aggctctgga acattgcaac
     2761 ccgtttcgct ggcgaggcgc cctatttcat gcgcctggga ccttcaaggc ctgataatcg
     2821 atcatcacgt ggtcctgata cgccctgcgc tcggtgagac gctgatacca ggcttccacg
     2881 ttctcaagcc gcggac
//
LOCUS       DASUMG010000032         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_967768, whole genome shotgun sequence.
ACCESSION   DASUMG010000032 DASUMG010000000
VERSION     DASUMG010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967768"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFX46_00575"
     CDS             <1..667
                     /locus_tag="VFX46_00575"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599110.1"
                     /translation="ENGLASGDKVKRIIDLSTTGPQTAEKIANAFKARSVRWLDCPVS
                     GGVGGAKAATVAVMFSGPREDYDDLLPVLQVIGKPFYIGDKPGLAQMMKLVNNCLSAA
                     AMALTSEAAVVGAKAGIPPDVMIDVINAGSGRNTATLQKFPQSILPGTFDYGFATGLM
                     HKDVKLFIEEARRMGLKLEGCEVVLDLWQKALDKLGPDSDFTRIVTLMEEQAGVEVRS
                     KKA"
     gene            782..1180
                     /locus_tag="VFX46_00580"
     CDS             782..1180
                     /locus_tag="VFX46_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5599111.1"
                     /translation="MEIIRSGSRPTRRAPASSFTGTVWQDPIIEAPAPALLKSNVVTF
                     EPGARTAWHTHPMGQTLYVLHGTGRIQAWGGPIQEIKAGDVVWIPPGEKHWHGAAPDT
                     MMIHISMQEGRDGVVVEWLEHVTDEQYMGG"
     gene            1304..1702
                     /locus_tag="VFX46_00585"
     CDS             1304..1702
                     /locus_tag="VFX46_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599112.1"
                     /translation="MAEFPDEGWMEAFRETVARDPELAVIGHWFNVDMGLVFGERRYV
                     LKVRKGKIEEIIPSPRFDVRTQFQMRAPMAVWQRFLSEPPPPLYHDIFAMIMRVPEFV
                     LEGDTLVAMQNARALHRMMGLMRSVGRVHA"
     gene            1695..>2273
                     /locus_tag="VFX46_00590"
     CDS             1695..>2273
                     /locus_tag="VFX46_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5599113.1"
                     /translation="MPEFEPIIGRYLKVSIQGVDHRVFVEEAGQGIPLICLHTAGADS
                     RQYRHLMNDTAVTDRFRVICFDMPWHGRSDPPDKWWLQTYQLTTESYLATIEAVWRAL
                     EVERPVFLGCSMGGAIVLRIAGDFQDEIRGVIGLESAAYAPGRYNEFLHHPAIHGGEL
                     VATYTWGLNAPQSPEAGKRANWWYYAQSGPGVY"
BASE COUNT          447 a          682 c          730 g          414 t
ORIGIN      
        1 ggagaatggt ctggcttcgg gcgacaaggt caagcggatc atcgacctct ccacgacggg
       61 cccccagacg gcagagaaga tagctaacgc tttcaaggcg cgtagcgtcc gctggctcga
      121 ttgcccagtg agcggcggtg tgggcggcgc caaggccgca acggtagccg ttatgttttc
      181 ggggccgcgt gaggactatg acgacctcct cccggtgctg caggtgatcg gcaagccgtt
      241 ctacatcggt gacaagccgg gcttggccca gatgatgaag ctcgtcaaca actgcctgtc
      301 cgccgcagcg atggcgttaa cgtcggaggc cgccgttgta ggcgcgaagg ccgggattcc
      361 acccgacgtg atgatcgacg tgatcaatgc gggcagcggg cgaaacacag cgactttgca
      421 gaagtttccc cagtccatcc tgccgggcac tttcgattac ggctttgcca ctggcctcat
      481 gcacaaggac gtcaagctgt tcatagagga ggcaagacgc atggggctga agctcgaagg
      541 ctgcgaagtc gttctcgatc tgtggcagaa ggcgctggat aagctcggtc cggattccga
      601 cttcacgaga atcgtcaccc tcatggagga acaggctggc gtcgaggtgc gtagcaagaa
      661 agcctgacct gcgcctcgct gattgcggat gcggccgact gcgggtttca agtgtccgcg
      721 ccagcgagaa ggcagtcagc accacgccaa tccggccctg cagcaagaag gaggaaagcc
      781 gatggagatt ataaggagcg gctcaagacc aacacgtcgc gcgccggcaa gctctttcac
      841 cggaacggtc tggcaggacc ccatcatcga ggcgcctgcg cctgcgttgc tgaagtcgaa
      901 tgtggtcacg ttcgagccgg gtgcgcgcac cgcttggcac acccacccga tgggccagac
      961 gctttacgtc ctccatggca caggccgcat tcaggcctgg ggcggcccaa ttcaggaaat
     1021 aaaggcgggg gatgtcgtct ggattccgcc cggcgaaaag cattggcacg gcgccgcccc
     1081 ggatacgatg atgatccaca tctccatgca ggaaggacgc gacggcgtcg tggtcgaatg
     1141 gctggagcat gtgaccgacg agcagtacat gggcggctga cgtccaaggc accgcagagc
     1201 ttcaaggctg gtccacgata acgaggggca ggagcgcggg tgtttctctc gagcaagacc
     1261 gcgctcatct gcaccgtggg aggaaccaac acaggagggc ggcatggctg agtttccgga
     1321 cgaaggctgg atggaagcct ttcgggaaac tgtggcgcgc gatccggagc tcgccgtcat
     1381 cggccactgg ttcaatgtcg atatgggcct cgtgttcggt gagcgccgtt acgtcctgaa
     1441 agtgcgcaag ggaaagatcg aggaaatcat cccttccccg cgcttcgatg tccgcacgca
     1501 gttccagatg cgggcgccga tggccgtgtg gcagcgcttc ttaagcgagc cccctccccc
     1561 gctctaccac gacatcttcg caatgatcat gcgcgtgccg gagttcgttc tggagggcga
     1621 cacgctcgtc gctatgcaga atgcgcgcgc gttgcatcgc atgatgggtc tgatgcgcag
     1681 tgtcgggagg gtccatgcct gaattcgagc ccatcatcgg gcgctacctg aaggtttcga
     1741 tccaaggggt cgatcaccgc gtcttcgtgg aggaagcggg acaaggcatt cccctcatct
     1801 gtcttcacac cgccggggcc gacagccgcc agtaccgcca cttgatgaat gacacggcgg
     1861 ttacggaccg gttccgcgtc atctgctttg acatgccctg gcacggccgc tccgatcctc
     1921 ccgacaagtg gtggctccag acttaccagc tgaccaccga gagctatctc gcaaccatcg
     1981 aggccgtgtg gcgcgctctt gaagtcgagc gcccggtgtt cctcggctgc tcgatgggcg
     2041 gggcgatcgt gctgagaatc gccggcgatt tccaggacga gatccgcggc gtgatcggcc
     2101 tcgaaagcgc cgcttacgcc ccgggacgtt acaatgaatt cctacaccat cccgccattc
     2161 atggcggcga gctggtcgcg acctacacgt ggggcctcaa cgctcctcaa agtccggagg
     2221 cgggcaaacg cgccaactgg tggtactatg cccagtctgg acccggcgtt tat
//
LOCUS       DASUMG010000033         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_566074, whole genome shotgun sequence.
ACCESSION   DASUMG010000033 DASUMG010000000
VERSION     DASUMG010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566074"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VFX46_00595"
     CDS             <1..1414
                     /locus_tag="VFX46_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX5599114.1"
                     /translation="EQIFGKGVTFKPARLFRMARSKAYLFGGVEIRWSCDPSLIKDDT
                     PAEAVFHFPGGLKDFLLATINGRTLVTKDIFTGSVETKDRHGSVEWAIAWLADEDGFC
                     NSYCNTIPTPEGGTHEQGLRQALTKSLRAYGELVGNRRAAQITADDVMGTAAAMLSVF
                     IREPEFQGQTKDKLATQEAARIVENTVRDAFDHWLADSPAQATRLLEWVVEQAEERIR
                     RRREKEVSRQSATRKLRLPGKLADCTIQSAKGTEIFIVEGDSAGGSAKSARERETQAV
                     LPLRGKILNVANASSAKLAQNQLLSDLILALGVGTKSKYREEDLRYERVIVMTDADVD
                     GAHIASLLITFFYQEMPQLIENGHLYLAVPPLYKITQGNVTAYARDDQHKEELLRTVF
                     NGRGKVEISRFKGLGEMNAQQLKETTMDPRRRTLLRVELGDGDRSALAETVNNLMGSK
                     PEARFRFIQERAPFAKDLDI"
     gene            1703..1969
                     /locus_tag="VFX46_00600"
     CDS             1703..1969
                     /locus_tag="VFX46_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599115.1"
                     /translation="MNRILAALALAASLALIPTQVTAQGTQSNAAPAQKASTAVKAGK
                     DEKVCRYRFPTGEVKTWVCKKEQPCCAWDLFSYVKCGSTITGCL"
     gene            2723..3637
                     /locus_tag="VFX46_00605"
     CDS             2723..3637
                     /locus_tag="VFX46_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5599116.1"
                     /translation="MMQLPFYTLDVFTEKRFSGNPLAVVLDADQLATEQMLAIARELN
                     LSETVFVQKPANPAHTAKVRIFTPGAEIPFAGHPTAGAAVLLAELRAPDPSEDLDAII
                     ALEENIGIVRVGVRLRRGQAAFAEFDAPKLPEQSGTLPPVESIAAAVGLLPREIGFAN
                     HRPTRFAAGNSFAFIPVASLEAMAKAEVNSQYWGAAMSGQGLTGAFLYTRETIHTTSS
                     FHARMFAPDLGVPEDPATGSAAVTFAGVIQMFDDLPDGHHKRVIEQGFEMGRPSIISL
                     TLEVEQGQLHNVRIGGNAVRVTEGRITI"
     gene            3658..>4005
                     /locus_tag="VFX46_00610"
     CDS             3658..>4005
                     /locus_tag="VFX46_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599117.1"
                     /translation="MMNSKLAIATIIACGLFQLLSPQSVSAEQVIWFGNRSEDGASLT
                     YGTPDSGYGKIVFSCEAGQDDVYFIYEHKPIIAEEGTQVEVFLAAGGVEIAIPTTGSR
                     LEIDDAFLLEGLTK"
BASE COUNT          799 a         1212 c         1242 g          752 t
ORIGIN      
        1 cgagcagatc ttcgggaagg gcgtgacctt caagccggcg cggctgttcc ggatggcccg
       61 ctccaaggcc tacttgtttg gaggcgtgga gatccgctgg tcctgcgacc cgtcgctcat
      121 caaggatgat acgccggctg aagccgtctt ccattttcct ggcgggctga aggacttcct
      181 gcttgccacc atcaacggac gcacgctcgt caccaaggac attttcacgg gctctgtcga
      241 aaccaaggat cggcacggct ctgtcgaatg ggccattgct tggttggctg acgaagacgg
      301 tttctgcaac tcctactgca acaccattcc gacgccggag ggtggcacgc acgagcaggg
      361 cctgcgccag gcgctgacga agagccttcg tgcctacggt gagctcgtcg gcaaccgccg
      421 tgccgcgcaa atcaccgccg atgacgtgat gggcacggcc gccgccatgc tctccgtgtt
      481 catccgcgag cctgaattcc agggccagac gaaggacaag ctggcgacgc aggaggccgc
      541 ccgcatcgtc gagaacaccg tccgcgatgc attcgaccac tggctcgctg attcgcccgc
      601 ccaggcaaca cggctgctcg aatgggtagt cgagcaagcc gaggagcgga tcaggcgtcg
      661 gcgcgaaaaa gaagtcagtc gtcagagcgc cactcgcaag ctgcggctgc ccggcaagct
      721 cgccgactgc accattcagt cggcgaaagg caccgaaatt ttcatcgtcg agggtgactc
      781 ggccggcggc tctgcgaaga gtgcccgcga gcgggagacg caggctgtgc ttcccctgcg
      841 cgggaagatc ctcaacgtgg cgaacgcctc ctcggccaag ctcgcgcaga atcagctgtt
      901 gtcggatctt attctggccc tcggcgtcgg caccaagtcg aaataccggg aggaggatct
      961 gcgctacgag cgcgtgatcg tgatgacgga cgccgacgtg gacggcgctc acatcgcctc
     1021 cctgctcatc accttcttct atcaggagat gccgcagctc attgaaaacg ggcatctcta
     1081 tctggcggtg ccgccgctct acaaaatcac gcagggcaac gtcacagctt acgcgcgcga
     1141 cgatcagcac aaggaggagt tgctgcgcac cgtcttcaac ggccgcggca aggtcgagat
     1201 cagccgcttc aagggtcttg gcgaaatgaa tgcgcagcag ctcaaggaaa cgacgatgga
     1261 cccgaggcgg cgtacgctgc ttcgcgtcga gctgggggat ggcgaccgta gcgcgcttgc
     1321 agagacggtg aacaacctca tgggctccaa gcctgaagcc cgcttccgct tcatccagga
     1381 acgcgctccg ttcgcgaagg acttggatat ctgatggtgt tcaagaccgc tgtcgcgcag
     1441 gctttggctg cgccggtttt gatcgctata cgggcgtgag ggtcgcggct tcgacaggcc
     1501 gcacgaaccg ccatcggaaa gcgggcgaat accctcaatc atcaaggcgc aaataggatc
     1561 agacgctccg tcctgcttct tttcctctgc tgcagtgtgc ccgccagaaa accggggatg
     1621 tgaggcttcc tggtcatagc agggaggtga tcgggatgcg catactactg atgtaatgtg
     1681 catcctttga ggagattgcc ttatgaaccg tattctggcc gcgcttgccc ttgccgcgtc
     1741 gctggccctc atccccaccc aagtgacggc gcaaggcaca cagtcgaatg ccgcgcctgc
     1801 gcaaaaagcc tccacggccg tgaaagccgg caaggacgag aaggtttgcc gttaccgctt
     1861 cccgacaggc gaggtcaaaa cctgggtttg caagaaggag cagccctgct gcgcctggga
     1921 cctcttctcg tatgtcaagt gcggatcgac catcaccggc tgtctatagc gccacggtcg
     1981 acacctaagc gcacattgag gaatcccccc ccgcataagt gaggccgagg tcgccggaag
     2041 acccatccca ccgcgtcggg gagggaggtg tgtggcggtg cggcagcaca ggccttccgg
     2101 cgagtccatc gcccgcgccg ctattgcggc tcacgagcta gaaaaggtcg ggcggcggag
     2161 aagcggggtt tccccaccgc ccgactcggg aatcgacatc tagcgccgaa tcccgagtct
     2221 cgggcttgtg ggccctgctc tcatccgggg cacttttgtg ccgcgcggag cgctgggccc
     2281 gcttgctcga tcaccggagt acagattgcg caaatgcacc ccggcgacgt gttgttctcg
     2341 ccggagcgta cgaacgcctc ggtttagggc gatatcgacc aggccccggc aacccgttcg
     2401 acatcgttaa tatcgcctcg cgaacgtggg ctgagcatga ccagcaaggg gcgtaattgt
     2461 ggctcgtctg atcaacttga ctgcaacata ttgtaacgat tgtgagcgcg cgaccgtcac
     2521 tcgctgtccc atcggggtgg aactgcggca gcacgacgac tcagtgtggc agaggaccat
     2581 ctcgatcggt gcatattcga gcaggaggca cgtggtgacc gcagctacac gcggcgcgac
     2641 gcagtgatcg gttcgccgaa ttctacttgg aagccggcac cacttgggcc aaaaagcgac
     2701 tgaaatcttc ggtccgagtg ggatgatgca gcttccgttc tacactctcg acgttttcac
     2761 ggagaagagg ttcagcggca atccgcttgc tgtggtgctc gatgccgatc agcttgcgac
     2821 ggagcagatg ctggcaatcg cacgcgagct caacctgtcg gaaaccgtat ttgtccagaa
     2881 gccggcaaac ccggcccaca cggccaaagt tcgcatcttc acgcctggcg cggaaatccc
     2941 gtttgccggt cacccgacgg cgggcgcggc cgtgttgctt gccgagctgc gcgcccctga
     3001 cccgagcgag gatctcgatg caatcatcgc actcgaggaa aacattggca tcgtacgcgt
     3061 gggagtcagg ctgagacgtg ggcaggcggc atttgcggag tttgacgcgc ccaagctgcc
     3121 ggaacagtcc ggcacgcttc cgccggttga gtcgatcgcg gccgcggtgg ggctgctccc
     3181 ccgcgagatc ggtttcgcga accatcgccc gaccagattt gcggccggaa acagcttcgc
     3241 cttcataccg gttgcatctc tagaggccat ggcgaaggcg gaggtgaaca gccagtactg
     3301 gggagctgcg atgtcgggcc agggcctcac cggcgcgttt ctttacaccc gcgagacgat
     3361 ccacacgacg tcctccttcc acgcccgcat gttcgcgccc gatctgggtg tgccagagga
     3421 tccggcgacg ggctccgccg ccgtgacatt cgcaggcgtg attcagatgt tcgacgacct
     3481 gcctgacggc caccacaagc gagtcataga gcaaggattt gagatgggcc gtccgagcat
     3541 catctcactg accctggaag ttgagcaagg tcagcttcac aacgtgcgaa tcggagggaa
     3601 tgccgtccgt gtcacggaag gtcgaatcac gatctgaata ctacggattt aagaaaaatg
     3661 atgaactcga agcttgctat cgccacaatc attgcctgcg gccttttcca gcttctttcg
     3721 ccacaatcgg tctcagctga gcaggtcatt tggttcggca atcggagcga ggatggggcg
     3781 tcgctcacct acggaacgcc ggatagtggc tatggaaaga tcgtgttcag ttgtgaggct
     3841 gggcaggacg acgtgtattt catctacgag cacaagccca taatcgcgga ggagggcacg
     3901 caagtcgagg ttttcctggc agccggaggc gtcgagatcg ctattccgac aactggatcg
     3961 cgcctcgaga ttgatgacgc ttttctgctc gaagggctta cgaag
//
LOCUS       DASUMG010000034         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_807244, whole genome shotgun sequence.
ACCESSION   DASUMG010000034 DASUMG010000000
VERSION     DASUMG010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807244"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VFX46_00615"
     CDS             <1..873
                     /locus_tag="VFX46_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5599118.1"
                     /translation="GAIKLARRYHHVCGRPERYRIITFQGAFHGRTLATIAAAGNEKY
                     LDGFGPRMPGFDVLPLGDMAAVEAAIGPETAGIMIEPVQGEGGIRVVPPEDLRRLREL
                     CDKHGLLLVLDEVQCGFGRTGKLFAHEWAGITPDVMAIAKGLGGGFPVGAFLATAEAA
                     KGMVPGTHGSTFGGNPLAMAVGNAVLDVVLEPGFLEAVQQKANRFKQELARIKDEHPD
                     VVEEIRGLGLLTGIRVKPPVADVLKACTAEKLLAVTAGENVLRLLPPLNITDAELSEG
                     IARLSRALRRVSTS"
     gene            913..1836
                     /gene="argF"
                     /locus_tag="VFX46_00620"
     CDS             913..1836
                     /gene="argF"
                     /locus_tag="VFX46_00620"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX5599119.1"
                     /translation="MAPRHFIDIGDIDSATLRRIVDTAHEMKRAGKVVPASLRPEGIE
                     DAVLVMIFEKPSTRTRVSFDVAMRQLGGQTIALNHTDLQLGRGESIADTARVLSRYID
                     VIMIRANKHESVLELAQYSSVPVINGLTDKSHPCQVIADIMTFEEHKGPIKGQTVAWV
                     GDGNNVATSWMHAAARFGFRLRIASPAQLRPEKQVLDWVKSEGADVLVTDDPEEAVAG
                     ADCVVTDTWVSMGHTDASRRKQILGPYAVNGELMNKASKDAIFMHCLPAYRGNEVSAE
                     VIDGPQSVVFDEAENRLHAQKAILAWCLGKD"
     gene            1837..2811
                     /locus_tag="VFX46_00625"
     CDS             1837..2811
                     /locus_tag="VFX46_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone"
                     /protein_id="HEX5599120.1"
                     /translation="MAERLMLTNTGGLRKTADDLIMPFRTEQSGVFGRLVRLGPVINS
                     VLNRHDYPEPVSRVLGEAVALTALLGSSLKFDSAYGGRLILQTKTDGPISFLVVDFEE
                     PGRVRGYASFDREKVEGLGRVGWNEPGRLLGNGHLAMTIDPGGDLDRYQGIVPLDGCG
                     LSDAARVYFRQSEQIPTFLRLAVARHYAGGTWQWRAGGLLVQYVPPLEEGATSNDENE
                     GLLLGEEDDRWTSARTLAETVEDHELLDPTLMPERLLYRLFADEGVRAFPSVSLEEHC
                     RCSRDRVSNLLGSFGADELADMREADGAITVTCEFCNTKYRFEIDEIR"
     gene            complement(2902..>2997)
                     /locus_tag="VFX46_00630"
     CDS             complement(2902..>2997)
                     /locus_tag="VFX46_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HEX5599121.1"
                     /translation="AAKSAIMGALSLYLDFINMFTSLLYLFGERE"
BASE COUNT          557 a          889 c          984 g          567 t
ORIGIN      
        1 ggcgccatca aactggcgcg ccgctatcac cacgtctgcg gccggcccga gcgctaccgg
       61 atcatcacat tccaaggtgc cttccacggc cggacactcg cgaccatcgc tgcggccggc
      121 aatgagaagt atctcgacgg cttcggtccg cggatgcctg gcttcgacgt gctgccgctg
      181 ggcgacatgg ctgccgttga agcagcgatc ggccccgaaa cggcgggcat catgattgag
      241 cctgtacaag gcgaaggcgg cattcgggtc gtccccccgg aggatctccg gcggttgcgc
      301 gagctatgcg acaagcacgg gctgctgctc gtgctggacg aggtccagtg cgggttcggc
      361 cgcacgggca agcttttcgc gcacgagtgg gcggggatca cgccggatgt gatggccatc
      421 gccaagggcc ttgggggggg atttcctgtc ggagcgtttc ttgccaccgc ggaggccgcg
      481 aagggcatgg tgcccggaac tcacggctcg accttcggcg gcaatccgct cgctatggcg
      541 gtcggcaacg ccgttctgga cgtcgttctc gagccagggt tcctggaggc ggttcagcag
      601 aaggccaacc gtttcaagca ggagctcgca cggatcaagg acgagcaccc tgacgtcgtc
      661 gaggaaatcc gtggtctcgg gcttctcacc ggcattcgcg tgaagccgcc agttgccgac
      721 gtgctcaaag cctgcaccgc ggagaagctg cttgcggtga cggccggcga gaacgttctg
      781 cggcttctgc cgcccctcaa cattacagac gcggagctgt ccgaaggcat tgcgcgcctc
      841 tcgcgggcgc tccggcgtgt gtcgacgtcc taaccatgcg gcggaattcg ccccgccacc
      901 cgaggtcccg tcatggcgcc acgccatttt atcgacattg gagacatcga ctcggcaacc
      961 ctgcggcgga tcgttgatac ggcccacgag atgaagcgag ccggcaaggt ggtgcctgca
     1021 tcgctccggc ccgagggcat cgaggacgcc gtgctggtga tgattttcga gaagccatcg
     1081 acgcgcacgc gggtgtcttt cgatgtggcg atgcgtcagc tcggcggcca aaccatcgca
     1141 ctcaaccata cggatctgca gctcgggcgg ggggagtcca ttgcggatac ggcccgcgtc
     1201 ctctcgcgct acatcgatgt gatcatgatc cgtgccaaca agcacgagag cgtgctcgag
     1261 ttggcccagt attcctcggt tcccgtgatc aatgggctga ctgacaagag ccatccctgc
     1321 caggtcatcg ccgacatcat gaccttcgag gagcacaagg ggccgataaa gggtcagacg
     1381 gtcgcgtggg ttggggatgg caacaacgtc gcgacctcct ggatgcacgc tgccgcgcgg
     1441 ttcgggttcc gcctgcggat cgcgtcgccc gcccagcttc ggcctgagaa gcaggtgctc
     1501 gactgggtca agtcggaggg agctgatgtg cttgtcaccg acgaccccga agaggccgtg
     1561 gctggggccg actgcgtcgt tacagacacg tgggtttcaa tgggccatac cgatgcgtcc
     1621 cggaggaagc agatcctggg accatatgcc gtcaacggcg agctgatgaa taaggcgagc
     1681 aaggacgcca tattcatgca ttgccttccg gcttatcgcg gtaacgaggt cagcgcggaa
     1741 gtgattgacg gaccgcagtc cgtcgtgttc gacgaagctg aaaacaggct ccatgcacaa
     1801 aaagcaattc ttgcatggtg ccttgggaag gattgaatgg cagaacgcct catgctcacc
     1861 aacacgggtg ggctgcgaaa gacggcagat gacctgatca tgccgttccg gacagagcag
     1921 tccggtgtct tcggacggct ggtgcgcctc ggcccagtga tcaacagcgt tctcaaccgg
     1981 cacgattatc cggagcccgt aagccgcgtt ctcggagaag ccgtggcgct gaccgctttg
     2041 ctgggctcct cgctcaaatt cgacagtgcc tacggcgggc ggctcattct ccagacgaag
     2101 accgatgggc cgattagctt tctggttgtc gatttcgagg agcctggtcg cgtgcggggc
     2161 tacgccagct tcgacagaga gaaggtcgag gggctcgggc gcgtcgggtg gaacgagccg
     2221 ggtcgtcttc tcgggaatgg tcacctagct atgacgattg acccaggtgg tgacctcgat
     2281 cgctatcaag gcatcgtgcc gctcgatggc tgtgggctca gcgacgcagc ccgggtctat
     2341 ttccggcagt cagagcaaat acctactttt ctccggctag cggttgcccg ccattatgcg
     2401 ggaggcacat ggcagtggcg ggccggcggc ttacttgttc agtacgttcc gccgctcgag
     2461 gagggggcca cctccaacga cgagaacgag ggcctgctgt tgggggaaga ggacgaccgg
     2521 tggaccagcg ctcgcacctt ggccgagacc gtggaggacc acgagctgct cgatccgacg
     2581 cttatgcctg agcggctgct ttacaggctg tttgcggacg aaggcgtgcg cgccttcccg
     2641 tctgttagcc tggaggagca ttgccgctgc tcccgggatc gggtgagcaa tcttcttggg
     2701 agcttcggtg cagacgagct agcggacatg cgcgaggcgg acggagccat cacggtcacg
     2761 tgcgagttct gcaataccaa gtaccgcttc gagatcgacg agatcaggtg agggcggtac
     2821 ccacctccat cgctctatat catatgaaaa gggccccgtt tggcggggcc cttcttttca
     2881 tcattatatc aaccgttgtt ctcactcgcg ctcgccgaac agatagagca gcgaggtgaa
     2941 catattgatg aagtcgagat agaggctcag agcacccatg atggccgact ttgcagc
//
LOCUS       DASUMG010000035         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_4066, whole genome shotgun sequence.
ACCESSION   DASUMG010000035 DASUMG010000000
VERSION     DASUMG010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4066"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VFX46_00635"
     CDS             complement(<1..798)
                     /locus_tag="VFX46_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEX5599122.1"
                     /translation="MADKERTPAEELLSTQPKVVNVGLEAFVRDLKNAGIEVVHVDWA
                     PPAGGDPEMAALLAKLGSEESAINLIEDANREAIKRILEGEPKLLDVVPAREAIAGLK
                     ERMILHAGPPITWDRMCGPMRGAIAGAIVFEGWAPDLASAEKMAAAGEIEFHPNHHFG
                     CVGPMTGMTTMSMPVFVVENVRFGNRAYCAINEGLGKVMRFGGNDETVLARLRWLRDV
                     FGPELSKALRLLGGIELKSLVARGLSMGDELHQRNVACSSIFLREIAP"
     gene            complement(1025..>2185)
                     /locus_tag="VFX46_00640"
     CDS             complement(1025..>2185)
                     /locus_tag="VFX46_00640"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5599123.1"
                     /translation="VPVPRTLHNNATAKLQELRDVPGFAETYLFDGEPPPVGSRLRQP
                     RLADTLDQLARAGLGDFYDGDLARSMAGDLERLGSPLRLEDFQRHQAVEVEPLSITAA
                     GHKIFNLPPPTQGLASLLILALYARNPAAVADGPDHLHRLVEATKAAFRVRNAHVTDP
                     DYMTQRAEEFLTDEFISKLSAGLSADRAAPWPEPPKQGDTVWLAAADSSGLCVSFIQS
                     IYWEFGSGVVLPETGILWQNRGTSFELNPNGTNALEPGRLPFHTIQPAMAELSDGRLM
                     AYGTMGGEGQPQTQAALFSRYVLHGQDLQTSVTAPRWLLGRTWGEESTNLKLESRFDP
                     ATIETLIARGHDVQVVGPFEEMMGHAGALVRHPDGLLEGASDPRSDGGVAAR"
BASE COUNT          423 a          681 c          630 g          451 t
ORIGIN      
        1 aggggcgatc tcgcgcagga atatgctcga acaggctacg tttctttgat gcagctcgtc
       61 ccccatggaa aggccgcgcg caaccaatga cttgagctcg atcccaccga gcagacgaag
      121 tgctttcgac agctcaggac cgaacacgtc ccgcaaccag cgcagtcggg ccagaacggt
      181 ttcatcattg ccgccgaagc gcataacctt gccgaggcct tcgttgatcg cgcagtacgc
      241 acgatttccg aagcggacat tttcaacgac aaaaaccggc atagacatcg tcgtcatgcc
      301 ggtcattggc cctacgcacc cgaaatggtg attaggatga aattcaatct ctccggccgc
      361 tgccatcttt tcagccgaag ccaaatcggg cgcccatcct tcgaatacga tggctcccgc
      421 aatggcgccg cgcattgggc cgcacatccg gtcccacgta attggtggac cagcgtgcag
      481 gatcatccgc tccttcaagc ctgcgatcgc ttcgcgcgcc ggtacaacgt cgaggagctt
      541 gggctcgcct tcgagaatgc gctttatggc ctctctgttg gcgtcttcga tcaaattaat
      601 cgcgctctcc tcacttccga gtttcgcaag aagagcagcc atctccggat caccgcctgc
      661 cggaggcgcc caatcgacgt gaaccacttc gatgccggcg ttttttaggt ctctcacgaa
      721 ggcttccagg ccaacgttga cgaccttcgg ctgagtgctc aacagctcct cagcgggggt
      781 cctctccttg tccgccactc tgcacctccg tggagcgatc gtcggttgaa ccacgttagt
      841 aagaagtttc ttactttgtc aatctgtatg cgggctctgt catcgggcta tgaccgatga
      901 tgccaagtcc gataaatggc agcgctccct tgatagcctg ctcatatttg aggccgtccc
      961 cggggattag accggctcac ctaatatcga ccaaccgctg caggcgagcc ttcttgtctc
     1021 ggaatcaacg cgcggcgact ccaccgtcgc tgcgagggtc gctggcgcct tcgagcaggc
     1081 cgtcgggatg ccgcaccagg gcaccggcat gccccatcat ctcttcgaat ggtccaacga
     1141 cctgcacgtc gtggccgcgc gctatcagcg tctcgatcgt tgcgggatcg aaccgcgact
     1201 ctagtttgag atttgtgctc tcttcgcccc acgtgcgccc gagcagccat cgcggagccg
     1261 ttaccgaggt ctgcagatcc tgcccatgga ggacgtagcg cgagaagagc gccgcctgtg
     1321 tctgcggctg cccttcgccg cccatggtgc cgtaagccat caagcggccg tcgctcaatt
     1381 cagccattgc aggctggatc gtatgaaacg gaagccggcc cggctccaaa gcattcgtgc
     1441 cattcggatt aagctcgaaa cttgtcccgc gattttgcca caggatgccc gtttcgggca
     1501 gcaccacgcc ggagccgaat tcccagtaga tactctggat gaagctaacg cagaggccac
     1561 tgctgtcagc ggcggcgagc caaaccgtgt cgccctgctt cggcggctcg ggccaaggcg
     1621 cagcgcgatc agctgacagg ccggcggaga gcttggagat gaattcgtca gtcaggaatt
     1681 cctccgcccg ttgcgtcatg taatcagggt ccgtgacatg agcatttcgg actcggaagg
     1741 cagccttcgt cgcttcgacc aagcggtgaa ggtgatcagg tccgtcggct acggcggccg
     1801 gattgcgggc gtagagcgcc aatatcagca aggatgccag cccttgggtc ggcggtggca
     1861 gattgaaaat cttgtgtccg gcggctgtga ttgataacgg ctcgacctca accgcttgat
     1921 ggcgttggaa atcctcaaga cgcaatgggc tgcccagccg ctcgagatcc cctgccatgg
     1981 atcgcgcgag atcgccgtcg tagaagtcgc cgaggccggc gcgcgctaat tggtctagtg
     2041 tgtccgccag tcttggctgg cgcaaccgtg acccgacggg aggtggctct ccgtcaaaaa
     2101 gataggtctc agcgaaaccc ggtacgtcgc gcaattcttg gagcttcgcc gtcgcgttat
     2161 tatgcagcgt ccgcggtacg ggcac
//
LOCUS       DASUMG010000036         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_245029, whole genome shotgun sequence.
ACCESSION   DASUMG010000036 DASUMG010000000
VERSION     DASUMG010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245029"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFX46_00645"
     CDS             <1..217
                     /locus_tag="VFX46_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEX5599124.1"
                     /translation="VFSILTTQPNSLVATINHERMPVLLRSEDEFETWLSGTPQEAFS
                     LVRCVEANAMRIVQSGADREDMLADQA"
     gene            536..1336
                     /locus_tag="VFX46_00650"
     CDS             536..1336
                     /locus_tag="VFX46_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HEX5599125.1"
                     /translation="MLRSVASAVVGVAAAGAFLASPAFADGGYPIYGDGGFKDEVHFA
                     PPPITWAGFYIGLHAGYGWADSDWKALEDIPPVLAGDRFSHDPEGGLFGGHIGYNAQT
                     GPVVWGIEASISGADINESSRTTFGGLIDPPPIHLRTDIDTLWSVTGRIGYDWGRLLT
                     YAKAGYAGADVELTVTDPLAFRVSESKTHHGWTVGAGIEYLASENLVLGLEYNFYDFG
                     DEDYGRRFALVNPNGVVENDVELHTITARVSYKFGRRAPIAEPLPPLK"
     gene            complement(1469..>2143)
                     /gene="ilvC"
                     /locus_tag="VFX46_00655"
     CDS             complement(1469..>2143)
                     /gene="ilvC"
                     /locus_tag="VFX46_00655"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX5599126.1"
                     /translation="LIEPKKTVDVVMVAPKGPGHTVRGEYQKGGGVPCLIAINQDASG
                     NAHDIALSYASGVGGGRSGIIETTFREECETDLFGEQVVLCGGLVELIRAGFETLVEA
                     GYAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEFGEYMSGPRIITKETREEM
                     KRVLKDIQTGAFTSEWIRECKAGQPKFKAIRRLNDTHQIEEVGAKLRAMMPWIKEKAL
                     VDKSRN"
BASE COUNT          386 a          683 c          632 g          442 t
ORIGIN      
        1 tgtgttcagc atcctcacga cgcagccgaa ttcgctcgtg gcgacaatca accacgaacg
       61 gatgcccgtt ctgctgagaa gcgaggatga gttcgagacc tggctcagcg gaacgccgca
      121 ggaggcattc tcgcttgtcc gctgcgtcga ggctaacgcc atgcgcatcg tgcagtccgg
      181 cgccgaccgc gaggacatgt tggcagatca agcataaagt tgcaggtaac aaattcactt
      241 ttctgtctag cggcaaagtt gttgataatc gggttttgca caacctgtag tcgcatcttc
      301 gccactgcga ccgcccggca acactctcca agtgcccgtc cccattgagt tctaagcgcg
      361 ttgggctcct caggcctaac gcatgcttaa gacttgccct ctattctatc tctggttgcg
      421 ttgatcgctc tgctcgcgtt ggagtacgag tcagaccacg gaccgagggg agcggtcccg
      481 ccaagagcca gtttgtcagt agctcagcga gctctgctgt atggagggac tttcagtgtt
      541 gaggtcagtt gcatcggccg tagtcggcgt agcggccgcc ggagcattcc ttgcgtcgcc
      601 cgcgtttgcc gacggcgggt atccgatcta tggcgacggc ggtttcaagg atgaagtgca
      661 ctttgcacca ccgcccatca catgggccgg gttctacatc ggcctgcatg ccggctacgg
      721 gtgggcggat tccgattgga aggccctcga ggacatacct cccgtcctgg ccggcgaccg
      781 attcagccat gatcccgaag gcggcctctt cggcgggcac atcggataca acgcccagac
      841 cggcccggtc gtctggggta tcgaggcttc gatttcaggg gccgatatca acgagagctc
      901 gcgcacgacc ttcggtgggc tcatcgaccc gcctccgata catctcagga ccgacatcga
      961 cactctctgg agcgtgacgg gtcggatcgg atatgactgg ggtcgcctgc tcacttacgc
     1021 caaagccggt tacgccgggg ctgacgtcga gcttacagtc accgacccgc tcgcgtttcg
     1081 ggtttccgaa agcaagacgc atcacggttg gacggtcggc gccggcatcg aatacctcgc
     1141 ctccgagaac ctcgttcttg gcctggaata caacttttat gactttggcg acgaggacta
     1201 cgggaggagg ttcgctctcg ttaatcccaa tggggtagtt gagaacgatg tcgagcttca
     1261 caccatcacg gcgcgcgtga gctacaagtt tggccgtcgc gcaccgattg cggagccgct
     1321 cccaccgctc aagtgaccta acacctcccc ccacctgggc cgcgcgccgc cttagtcggc
     1381 ctcggctcag gcgcctctga acgccgcggc ccagctccag ggttacggca aactgaaagg
     1441 gctccggaat ccggagccct cttttccctt agttccggct cttatcgacg agagccttct
     1501 ccttgatcca aggcatcatg gcgcgcagct tggcgccaac ctcctcgatc tgatgggtgt
     1561 cgttgaggcg acggatggcc ttgaacttcg gctggccggc cttgcactcg cggatccatt
     1621 cgctggtgaa ggcgccggtc tggatatcct tcagcacgcg cttcatctcc tcgcgcgtct
     1681 ccttggtgat gatccgcggg ccggacatgt actcgccgaa ctcggccgtg tttgagatcg
     1741 agtagttcat gttcgcgatg ccgccctcat agatgaggtc gacgatcagc ttcacctcgt
     1801 ggaggcactc gaagtaagcc atctcaggcg catagcccgc ctcgaccaga gtctcgaacc
     1861 cggcgcggat gagctcgaca agcccgccac acagcaccac ctgctcgccg aagaggtccg
     1921 tctcgcactc ctcgcgaaac gtcgtctcga tgatgccgga gcgcccgccg cccacgcctg
     1981 aggcatagga gagcgcgatg tcatgggcat tgcccgacgc atcctggttg atggcgatga
     2041 ggcagggcac gccgccgccc ttctggtatt cgccgcgcac cgtgtggccg ggacccttcg
     2101 gcgccaccat gacgacgtcc accgtcttct tgggctcgat cag
//
LOCUS       DASUMG010000037         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_486120, whole genome shotgun sequence.
ACCESSION   DASUMG010000037 DASUMG010000000
VERSION     DASUMG010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486120"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFX46_00660"
     CDS             complement(<1..797)
                     /locus_tag="VFX46_00660"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014284329.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX5599127.1"
                     /translation="MNTQGRPTTPGAVSPATEGRTFTGHRGLDHEEPLIFEAGSLSKS
                     GVDLPEPKPVASRLNGLERQGPIGLPGLTEPEAMRHYVRLSQRNYAIDTGLYPLGSCT
                     MKHNPRLNEKMARLPGFSDIHPLQPQQTVQGALQLMEMLAAYLLELTGMKAVALSPKA
                     GAHGELCGMLAIKAAHEAAGSKRDVVLVPDSAHGTNPATAAFMGYSVRSIPAREDGTV
                     SVEAVRAALGPDVAAIMLTNPNTCGLFEPEVVEIAKAVHEAGAYFYC"
     gene            complement(803..2146)
                     /gene="gcvPA"
                     /locus_tag="VFX46_00665"
     CDS             complement(803..2146)
                     /gene="gcvPA"
                     /locus_tag="VFX46_00665"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX5599128.1"
                     /translation="MRYLPLEQADREAMLAKIGVSSVDELFASVPADKLLKELPNLPR
                     AKGELEVERELSKMAARNVPAGSVPFFVGAGAYRHHVPATVDHLIQRSEFLTSYTPYQ
                     PEISQGTLQALFEFQTQVALLTGMEVANASMYDGSTACGEAVLMAHRLTRRKKALLSG
                     GLHPHYRAVVETLAHWGGSTVVALDPDPTGVEDITSKIDEETSCAVVQYPSFYGNIRD
                     LTPIAEAAHAKGALLIAVVTEIVSLGLLKPPGEMGADIVAAEGQSLGNGLNFGGPYVG
                     LFATREKFVRQMPGRLAGETVDAEGRRGFVLTLSTREQHIRREKATSNICTNSGLCAL
                     AFTIHLTLLGEAGLRKLARINHANAVTLADELGKVRGVEVISPSFFNEFTIRVPGDAR
                     KVIDALAEKGVMGGVPVSRLEPKRSELRDLIIVASTEINTDEDRAAYVKALQEVL"
BASE COUNT          389 a          743 c          718 g          404 t
ORIGIN      
        1 tcgcagtaga agtacgcgcc ggcctcatgc accgccttcg caatctcgac cacctccggc
       61 tcgaacaggc cgcaagtatt cggattggtc agcatgatgg ccgcaacatc aggacccagc
      121 gccgcgcgga cggcttcgac ggagacagtg ccatcttccc gcgccggaat ggagcgcacc
      181 gagtagccca tgaaagcagc cgtcgccggg ttggtgccgt gcgccgagtc cgggaccagc
      241 acgacgtcgc gcttcgagcc ggctgcttcg tgcgccgcct tgatggcgag catcccacaa
      301 agctcgccgt gagcgcccgc cttcggtgaa agggcgaccg ccttcatgcc cgtgagctcg
      361 agcaggtagg ccgccagcat ctccatgagt tgcagcgccc cctgcaccgt ctgctggggc
      421 tgcagcggat ggatatcgct gaagcccggc agccgcgcca ttttctcgtt gagacgcggg
      481 ttgtgtttca tcgtgcagga accgagcggg tacagtcccg tgtcgatcgc gtaattccgc
      541 tgggacaggc gcacgtaatg gcgcatggcc tccggctcgg tcagtcccgg caggccgatc
      601 ggcccttggc gttccagacc gttaagacgt gacgctaccg gcttcggctc cggaagatcg
      661 acaccgctct tcgacaggct gccggcctcg aagatcagcg gctcctcgtg gtcgaggccc
      721 ctgtggcccg tgaacgtacg gccttcggta gccggagaaa ctgcgcccgg agttgtaggg
      781 cgaccttgag tattcatacc catcagagca cctcctgcag cgccttgacg taggccgcgc
      841 ggtcctcgtc ggtgttgatc tcggtggatg caacgatgat caaatcacgg agctcgctgc
      901 gcttgggctc cagccgcgaa accggcacgc cgcccatcac gcccttctcg gccagcgcat
      961 cgatgacctt gcgagcatcg ccgggaacgc ggatggtgaa ctcgttgaag aagctcggtg
     1021 agatcacctc gactccccgc accttgccga gctcgtcggc cagcgtgacg gcattcgcgt
     1081 gattgatgcg cgccagcttc ctgagccccg cctcgccgag cagcgtcagg tggatggtga
     1141 acgctagcgc gcacaagcca gagttcgtgc aaatgttcga ggtcgccttt tcccggcgga
     1201 tgtgctgctc acgtgtcgaa agggtgagca cgaatccgcg ccggccctcc gcatccacgg
     1261 tttcgcccgc gaggcggccc ggcatctgac ggacgaattt ttcccgcgtt gcgaatagcc
     1321 cgacataggg tccgccgaaa ttgagaccgt tgccgaggga ctggccttcc gctgccacga
     1381 tatcggcgcc catctcccct ggcggtttga gcaggccgag cgagacaatc tcggtgacga
     1441 cggcgatcaa cagggcgccc ttggcgtggg ccgcttcggc aatcggcgtc agatcgcgga
     1501 tgttgccgta gaagctcggg tattggacga ctgcgcagga cgtttcttca tcgatcttgg
     1561 aggtgatgtc ctcgacgccc gtcgggtcag gatcgagcgc cacgaccgtc gagccgcccc
     1621 agtgcgcgag agtttcgacc accgcgcgat agtggggatg cagcccgccc gacagcagcg
     1681 ccttcttccg ccgtgtgaga cgatgggcca tgagcaccgc ttcgccgcac gcagtcgagc
     1741 cgtcgtacat ggaagcgttg gcgacttcca tgccggtcag cagggccacc tgcgtctgga
     1801 actcgaacag cgcctgcagc gtgccctggc tgatttcggg ctgatacggc gtatacgaag
     1861 taaggaactc ggaacgctgg atcaggtgat cgaccgtcgc cggaacgtga tggcggtagg
     1921 cgcctgcacc gacgaagaac ggcaccgagc ccgcaggcac gttgcgcgcc gccatcttcg
     1981 acagctcgcg ctcgacctcc agctcacctt tggctcttgg caggttcggc agctccttca
     2041 ggagcttgtc ggccggcacg ctcgcgaaaa gctcgtccac ggacgagacg ccgatcttgg
     2101 cgagcatggc ctcacgatcg gcctgctcga ggggtaggta acgcattagc accgctccat
     2161 cggtacgtcg ctcgtaggtg ccctcccgtc atcccgacgg caaagagttc aggaggagag
     2221 actgcgcttg gctcgcgcgc tgcccccacc tgcc
//
LOCUS       DASUMG010000038         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1128758, whole genome shotgun sequence.
ACCESSION   DASUMG010000038 DASUMG010000000
VERSION     DASUMG010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128758"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VFX46_00670"
     CDS             <1..564
                     /locus_tag="VFX46_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX5599129.1"
                     /translation="LARLATSHAKEAGPDGLGLAAACATALGSAAVTEALGLHHVLGA
                     FLAGIAMPGAARRAILVRLEAPVAVALMPFFFVLTGLRTTIDLGSDTFVAVFLLMTLA
                     ATVGKFGGTALLARAAGKGWPTALGLGALMQTKGLMEVVVLTVVLDAGLIGPATFSAL
                     VLMALVSTAATMPLTRLMTRNEFSERS"
     gene            912..2807
                     /locus_tag="VFX46_00675"
     CDS             912..2807
                     /locus_tag="VFX46_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX5599130.1"
                     /translation="MRSPDNDKASGSPGSRLPILVIGAIGVVYGDIGTSPLYTMREAF
                     GLGGLPLGEATVLGVLSLVFWALLVVVTLKYVAVIMRADNQGQGGVLALVTLALRGLR
                     AGGRRQQLAIVLAMVGAALFYGDGIITPAISVLSAVEGLKVATPAFEPYVVPIAIAIL
                     VGLFLVQRRGTGGVGKLFGPIMCIWFATLAVLGIGQILDEPSVLRALNPIHAVALFAS
                     HGWQAFVALGAVVLAVTGAEALYADMGHFGRWPIRFAWFSFVLPALLLNYFGQGALLI
                     RDPGTVVNPFYLLAPPWALYPMVVLATWATIIASQAVISGAFSLTRQAIQLGYLPRLE
                     IRHTSEQEIGQVYLPRVNWALMVAIVAVVVGFGSSSQLAAAYGIAVVGAMSIDAVLAC
                     IVAVTLWRWHPLAAGLAFASFLAVDLAFFSANALKIPAGGWFPIAFAAAVFALMSTWR
                     TGREALFQRLYRGAPPLRSFLASVTRQPPTRVPGTAVFLTGNSDMVPRALLHNLKHNK
                     VLHERVVVLKVQTEDVPQVPEFERVQVEHLGGNFHRVLLRYGFLEHPNVTAALLQCQL
                     GAPEPTYMDTSFFLSRESLIPSEHPDLQPWREQIFMQMTNTALDATRYFRLPPDRVVE
                     IGRQVEI"
BASE COUNT          400 a          944 c          936 g          578 t
ORIGIN      
        1 ctcgcccgat tggcgaccag ccacgcgaaa gaggcgggcc cggacggact ggggctcgcg
       61 gccgcctgcg cgacggcatt gggctcggcg gcggtcaccg aggcgctggg cctgcaccat
      121 gtcctgggcg catttctcgc cggcatagcg atgcccgggg cggcgagacg cgcgatcttg
      181 gtgcggctcg aggcgccggt tgcggtggcc ctgatgccct tcttcttcgt cttgaccggc
      241 ctccggacca ctatcgacct gggctctgat acgttcgtcg ccgtcttcct gctaatgacc
      301 ttggccgcca ccgtcggcaa gttcgggggc accgcgctgc tggcccgtgc ggcgggcaag
      361 ggctggccga cggcgctcgg cctgggcgcg ctgatgcaga ccaaaggatt gatggaggtc
      421 gtggtgctca ccgtcgtgct cgatgccggc ctgatcgggc cagccacttt ctcagccctg
      481 gtgctcatgg ctttggtgag caccgccgcc acgatgcctc tcacccggtt gatgacgcgc
      541 aacgagtttt cggaacgctc ataagccgcg ggtgtcaact tcggccaggg tggtttcgtg
      601 ctcaagttcg gcggtggcca cttcgtgctc gggttcggtg tcagggcatg gcccgctcgc
      661 cgtgacgagc ctgcgcgact gctgagagaa tcgccgcaat cgccggctcg gtgcgcgaac
      721 cgggctacat ccgccccgcg caggttctct gatccggaga ctcggagcgc tttcgatcca
      781 ggtagtggcc gcggccgcgt gctgatcgca tcttgaaggg gcttgagggg aggaggctcg
      841 aggaccgtga tccaggccgt ggcactgcac gaaccgctcg ccgggcgcct cttatacgcg
      901 aggcctccat catgagatcc ccggacaatg acaaagcttc cggttctccc gggagcaggt
      961 tgccgatcct cgtcatcggt gcgatcggcg tcgtctacgg cgatattggc acgagccctc
     1021 tctacaccat gcgcgaggcg ttcgggctcg gggggctgcc gctcggcgaa gcgaccgtgc
     1081 tcggcgtgct ttctctggtc ttctgggcgc tgctcgtggt cgtcacgctc aaatacgtcg
     1141 ccgtcatcat gcgggcggac aatcagggcc agggtggcgt ccttgcgctt gtgacgcttg
     1201 ccctgcgtgg tttgcgcgcg ggtggacgac ggcagcagct ggccatcgtg ctcgcgatgg
     1261 ttggcgctgc gctgttctac ggcgacggca tcataacccc cgccatctcg gtgctcagcg
     1321 ccgtggaggg gctaaaggtc gcgacaccgg cgttcgaacc ttacgtcgtg ccgatcgcga
     1381 tcgcgattct ggtcggactc ttcctggtgc agcgtcgcgg cacagggggc gtcggcaagc
     1441 tgttcggtcc cataatgtgc atctggtttg ccacgctggc cgtgcttggg atagggcaga
     1501 tcctcgacga gccaagcgtt ttgcgggcgc tcaacccgat ccacgccgtc gccctgtttg
     1561 caagccatgg ctggcaagcc ttcgtggccc tcggcgctgt tgtgctggca gtgaccgggg
     1621 ccgaggcgct gtacgccgac atggggcact tcggtcggtg gccgattcgg tttgcctggt
     1681 tcagctttgt cctgccggcg ctcctgctca actacttcgg ccagggagcg ctcctgatcc
     1741 gcgacccggg cacggtggtc aatccctttt atctgcttgc gcctccgtgg gcgctttatc
     1801 ccatggtcgt gctggcgaca tgggcgacga tcatagcgtc gcaggctgtc atttcggggg
     1861 cgttctcgct tacccgccaa gcgattcagc ttggctatct tccgcggctc gagattcgcc
     1921 acacctccga gcaggagatc ggccaagttt acctgccgcg agtcaactgg gccctgatgg
     1981 tcgccatcgt cgcggtcgtg gttggcttcg gctcctcgag ccaacttgcc gcggcctatg
     2041 gcatcgcggt cgtcggcgcc atgagcatcg acgccgtcct cgcctgcatc gtggccgtca
     2101 ccctgtggcg ctggcatccg ctagccgccg gcctggcgtt cgcgtcgttc ctggccgtcg
     2161 acctcgcctt cttcagcgcc aatgcgctca agattcccgc cggcgggtgg ttcccgatcg
     2221 ccttcgctgc agcagtcttc gccttgatgt ccacctggcg caccgggcgc gaggcattgt
     2281 tccagcggct gtatcgaggc gccccgccgt tacgctcatt cctggccagc gtgacgcgcc
     2341 agccgccgac gcgcgtcccg ggcaccgcgg tgttcttgac cggcaattcc gacatggtgc
     2401 cgcgcgccct gctgcacaat ctcaagcaca acaaggtcct gcacgagcgt gtggtggtgt
     2461 tgaaggtcca aaccgaagac gtgccccagg tgccggagtt cgaacgggtg caggtcgagc
     2521 acctgggggg caacttccac cgtgtcctgc tgcgctacgg cttcctggag catccgaatg
     2581 tgacggccgc tcttctccaa tgccagctcg gcgcgccgga gcccacctac atggacacct
     2641 cgttctttct cagccgcgag agcctcattc cttcggagca cccggacctt caaccatggc
     2701 gtgagcagat tttcatgcaa atgacgaata ccgcgctcga cgccacgcga tacttcaggt
     2761 tgccgccgga tcgcgtggtc gagatcggcc gccaagtcga aatctagcgc gtggcgctgc
     2821 gacctaaagg ccgcgggcac agaacgctga atgcctcc
//
LOCUS       DASUMG010000039         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_4536, whole genome shotgun sequence.
ACCESSION   DASUMG010000039 DASUMG010000000
VERSION     DASUMG010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4536"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..751)
                     /locus_tag="VFX46_00680"
     CDS             complement(65..751)
                     /locus_tag="VFX46_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599131.1"
                     /translation="MSAARKILIVDDDEDLRDTLRDQLTLHDEFQVTTVADATSGMEA
                     VKNNHFDLIILDVGLPDMDGREACKALRKSGFKAPIIMLTGNASDADTILGLDAGAND
                     YVTKPFKFAVLLARIRAQLRQHEQSEDAVFTIGKYTFKPAAKLLIDEKGSKIRLTEKE
                     TSILKYLYRAGEKVVTRDVLLHEVWGYNANVTTHTLETHIYRLRQKIERDPGKAEILV
                     TEPGGYRLVP"
     gene            895..1407
                     /locus_tag="VFX46_00685"
     CDS             895..1407
                     /locus_tag="VFX46_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5599132.1"
                     /translation="MSSTRGWASIGGLRFPCALGRTGRRTIKREGDGATPVGAYVVRR
                     AFYRPDRLLRPTTRVPLRPLRPEDGWCDQVGDRNYNRYVKHPYPASAERMWREDHLYD
                     VVVVIDHNERPRVQGRGSAVFMHVARDGYEPTAGCIAFKRAHLLRILKLLHPGTTVRV
                     APWSRASAPR"
     gene            complement(1490..>2175)
                     /locus_tag="VFX46_00690"
     CDS             complement(1490..>2175)
                     /locus_tag="VFX46_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEX5599133.1"
                     /translation="VCALVLGPRIGYGTENMAPHNLVLTVIGASLLWVGWFGFNAGSA
                     AAADGGAGMAMAVTQIATAAAALAWMFSEWIVHRKPSVLGIASGAVAGLVAITPAAGF
                     VAPMGGLIIGIAAGVLCFIASTTVKRALGYDDSLDVFGIHGVGGIVGAILTGVFAVEA
                     IGGTPGLLEGNAAQVPLQIYGIIATIVWCAVATFVILMIVKAIMGLRVDKAVEIEGLD
                     INLHGEVVQ"
BASE COUNT          413 a          675 c          674 g          413 t
ORIGIN      
        1 ggcccgctcg aggctcgcgc gctgcgacat tagacgccgg atcagattat cgagcgcact
       61 caatctaagg caccagccgg tagccgcccg gctcggtcac gagaatctcg gccttgccgg
      121 gatcgcgctc gatcttttgc cgcagccgat atatgtgcgt ttcgagtgta tgtgtcgtca
      181 cgttggcgtt gtagccccag acctcatgca gcagcacgtc gcgggtgacg accttttcgc
      241 cggctcggta gaggtacttg agaatcgagg tctctttctc ggtgaggcgg atctttgagc
      301 ccttctcgtc gatgagcagc ttggcggcag gcttgaaggt gtacttgcct atggtgaaga
      361 cggcatcctc actttgctca tgctgacgga gctgggcccg gatgcgcgcc aacagaacgg
      421 cgaacttgaa gggcttcgtg acgtagtcgt tggcgcccgc atcgaggccc aggatcgtgt
      481 cagcgtccga ggcattgccg gtcaacatga tgatcggcgc cttgaagcct gacttgcgca
      541 aagccttgca ggcctcacgg ccatccatgt ccggcaggcc gacgtccaga atgatcaagt
      601 cgaaatggtt gttcttgacg gcttccatgc cgcttgtggc gtcagctacc gtggtgactt
      661 ggaactcgtc gtgaagggta agctggtcgc gcagggtgtc gcgaaggtcc tcgtcgtcgt
      721 cgacgatgag aatcttgcgt gccgcactca ttcgatctcg ctccccggat gttaggatgt
      781 tttgaagaat gtatcactgg agaggctgac gaaacagtgt ccggagtaag gccccggctg
      841 ccctgtaaga ttcatcgcaa ggcgcccgcc gagcttcaga tccgcgcgct gtccatgagc
      901 tccacccgcg gatgggcgag catcggcggg ctgaggtttc cgtgcgccct gggacggacc
      961 ggtcggcgca cgatcaagcg cgaaggagat ggcgccaccc ccgtcggtgc ctatgtcgtc
     1021 cgtcgtgcct tctaccgacc agatcgtctt ctgcgcccga ccactcgcgt gcccctccgg
     1081 ccactgaggc ctgaggacgg ttggtgcgat caggtcggcg acagaaacta caatcggtac
     1141 gtcaagcacc cctacccggc cagcgccgaa cgaatgtggc gcgaagacca cctgtatgat
     1201 gttgtcgtcg tgatcgatca taacgagcgg ccgcgcgtgc agggcagggg cagtgccgtg
     1261 ttcatgcacg tcgccaggga cggctacgag ccgacggccg gttgcattgc cttcaagcgc
     1321 gctcatctgc ttcggatatt gaagttgctg catcctggca cgacggtacg cgttgcgccg
     1381 tggtctcgtg cttcggctcc tcgatgacaa gtgaaaaagc ggcccggagg taatccgggc
     1441 cggtccagtc tagggagtag ttgagtggag aaatctgccg agcagcgcgc tactgcacca
     1501 cctcgccgtg cagattgatg tcgagaccct cgatctcgac tgccttgtcc acccgcagcc
     1561 ccatgatggc cttgacaatc atgaggatga cgaaggtggc tacggcgcac cagacgatgg
     1621 ttgcgatgat gccgtagatc tgcaggggaa cctgcgcggc attcccttcg aggagtccag
     1681 gcgtgccgcc aattgcctcg accgcgaata cgccggtcag gatggcgcca acgatgccgc
     1741 caacgccgtg aatgccaaac acatcgaggc tgtcatcgta gccaagcgct ctcttgaccg
     1801 tcgttgacgc gatgaagcag agcactccag cggcaatgcc gattatcagc ccacccatcg
     1861 gagcgacgaa cccagccgcc ggtgtgatcg cgacgaggcc agcgacagca cccgacgcga
     1921 tgccaagcac gctcggcttg cggtgcacga tccattccga gaacatccac gcgagcgcag
     1981 ctgcggccgt cgcgatctga gtcaccgcca tcgccatgcc cgctcccccg tcggcagcag
     2041 ctgcggaacc ggcgttgaaa ccaaaccagc caacccacag cagggacgcc ccgatcaccg
     2101 tcagcacaag gttgtgcggc gccatgtttt ccgtgccgta gccgatacgc ggcccgagga
     2161 ccagcgcgca gacaa
//
LOCUS       DASUMG010000040         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_727690, whole genome shotgun sequence.
ACCESSION   DASUMG010000040 DASUMG010000000
VERSION     DASUMG010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727690"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VFX46_00695"
     CDS             complement(<1..1077)
                     /locus_tag="VFX46_00695"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5599134.1"
                     /translation="MTAERSGPLKGMRVIDLSHVMAGPVCGLMLADMGADVIKVEKLE
                     GGDDTRRMLPPDINGEPAAFMMMNRNKRGIALDLKHPRGKEVLKRLVATADVLIENYR
                     RDTMERLGVGYETLKEINPGLIYCEISGFGRTGPLADQGGFDLVAQGMSGLMSITGEG
                     PGREPVKVGAPVTDITAGILGAMGVCAAYIHKLKTGEGQRVDTSLFEAGITHTYWQSA
                     IAFATGVSPGPMGSAHPLSAPYQAFPTKDGWINLGASNQRTWLKLVEVLDDAELAADP
                     RFKDNAGRMGNLPALVEVLTKHFKKRTTAEWLALLERAGVPAGPVLSIREMHVHPQTI
                     AREMVPEVEHPVAGKVQTIGLPVKF"
     gene            complement(1136..>2121)
                     /locus_tag="VFX46_00700"
     CDS             complement(1136..>2121)
                     /locus_tag="VFX46_00700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF004127.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX5599135.1"
                     /translation="NALTYPMVLAIHDALKRWEQDPEIAVVILDGAGDRALCAGGDVR
                     AIYESASEGSGFARKFWADEYRLNAYIGRYPKPYVAFMDGIVMGGGIGLSGHGSHRIV
                     TERSQLAMPETGIGLIPDVGGTWLLAHAPGETGIYLGLTGERMRGAGTMYARFADSFV
                     PSSRLADLAKALSEIENSSDVDSVIARFSEAPPPSDLASHAREIDATFCFDSVEKIRD
                     ALASMNTDWSRKTLEELDRRSPLALKLTLAAIRNARTLPSLEAALNVEYRLTVRLLEH
                     GEFLEGVRALLVDKDKTPKWNPPRLADVTDEMVAQFLSPLPPNEALELVAP"
BASE COUNT          373 a          670 c          668 g          410 t
ORIGIN      
        1 aaacttcacc ggcagtccaa tcgtctgaac cttgcccgcc accgggtgct ccacctctgg
       61 caccatttcg cgtgcgattg tttgaggatg cacgtgcatt tccctgatgg agagcacggg
      121 accagccggc acacccgcgc gttcgagcag cgccagccat tcggccgtgg tgcgcttctt
      181 gaagtgcttt gtgagcacct cgacgagggc gggcaaattc cccatccggc cagcgttatc
      241 cttgaagcgc ggatcggcgg cgagctcggc gtcgtccaat acctcgacga gcttgagcca
      301 ggtccgctgg ttcgaagcgc ccaggttgat ccagccgtcc ttggtcggaa aagcttggta
      361 gggcgccgaa agcggatgtg cggaacccat cgggcccggc gacacaccgg tcgcgaaggc
      421 aatggccgac tgccaatagg tgtgcgtgat gcccgcctcg aaaagcgagg tgtcgactcg
      481 ctggccctca cctgtcttga gcttgtggat gtaggcggcg caaacgccca tggcacctag
      541 gatgccggca gtgatgtcag tcaccggtgc accgactttc acgggctcac ggccagggcc
      601 ttcgcctgtg atcgacatga ggccggacat cccttgcgcc acgaggtcga agcctccctg
      661 atcggccaaa gggccggtgc ggccgaagcc ggaaatctcg cagtagatca ggccgggatt
      721 gatctccttc agcgtctcgt agccgacgcc gagccgttcc atggtgtcac ggcggtagtt
      781 ctcgatgagg acgtccgccg tcgcgacgag ccgcttcaga acctccttgc cccgtggatg
      841 cttcagatcg agcgcgatgc cccgcttgtt gcggttcatc atcatgaatg cggcaggctc
      901 gccgttgatg tccggcggca gcatgcggcg ggtgtcgtcg cccccttcga gcttctcgac
      961 cttgatgacg tccgcgccca tgtcggcaag catcagccca cagacggggc ccgccatcac
     1021 gtggctgagg tcgatcaccc gcatgccttt gagcggccct gagcgttcgg ctgtcattcc
     1081 tcgtcgcctc tctttttgcg cacggccgtg tttcacacct accgtggcac aggacctaag
     1141 gggcaacaag ctccagtgct tcattcggag gcaacgggct gaggaactgc gcgaccatct
     1201 cgtcggtcac atccgcgagg cgcggtgggt tccatttcgg cgtcttgtcc ttgtccacca
     1261 gaagagcacg gacgccttcg aggaactcgc catgctcgag gaggcggaca gtcagcctgt
     1321 attcaacatt tagggcggct tcgagcgagg ggagcgtccg cgcattgcgg atggccgcca
     1381 acgtgagctt cagagcaagt ggggagcggc ggtcgagctc ttccagcgtt ttccgtgacc
     1441 agtccgtgtt catgcttgcg agcgcatcgc ggatcttctc gacgctgtcg aagcagaagg
     1501 ttgcgtcgat ctcgcgggcg tgagacgcga ggtcagacgg cgggggcgcc tcgctgaagc
     1561 gagcgatcac gctgtcaacg tcggacgaat tttcaatttc acttagcgcc ttggcgaggt
     1621 ctgcaagccg tgaggacggc acgaaactat cggcaaagcg ggcatacatg gtcccggctc
     1681 cgcgcattcg ctcgccggtt aagcccaaat aaattccagt ctcgccgggc gcgtgcgcca
     1741 gcagccaggt gccgcccacg tcggggatga gtccaatgcc ggtttccggc atggcgagct
     1801 ggctgcgctc cgtgacgatg cggtggcttc catgcccgga caggcctatg ccgccgccca
     1861 tgacaatacc gtccatgaag gcgacgtacg gcttggggta gcggccgatg taggcattga
     1921 gccgatactc atcagcccag aacttgcgcg cgaagccgga gccctctgat gcgctttcgt
     1981 agatggcacg gacatcaccc cctgcgcaga gcgccctgtc acccgcaccg tcgagaatga
     2041 cgaccgctat ttccggatcc tgctcccagc gcttcagcgc gtcgtggatc gcgagcacca
     2101 ttggataggt cagcgcgttg a
//
LOCUS       DASUMG010000041         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_326255, whole genome shotgun sequence.
ACCESSION   DASUMG010000041 DASUMG010000000
VERSION     DASUMG010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326255"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /gene="polA"
                     /locus_tag="VFX46_00705"
     CDS             complement(<1..2402)
                     /gene="polA"
                     /locus_tag="VFX46_00705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX5599136.1"
                     /translation="MTLETAPQSVERVAECTVPEGEPVPIVKGSHVYLIDGSGYIFRA
                     FHALPPLTRPSDGLPVGAVYGFCNMLWKLLGDSKLSDRPTHIAVIFDTARDTFRTGIY
                     EAYKTNRPPPPEELVPQFGLIRRAVKAFNVACIEQDGYEADDLIATYACDALRKGGDV
                     TIVSSDKDLMQLIQPGITMLDTMKNKRIGREEVLERFGVEPEQVVDVQALSGDATDNV
                     PGVPGIGVKTAAELIREFGDLDTLLERAHEIKQPKRRERLIEFAEQARISRELVRLKC
                     DVPVKVPIERLGVQDPDPNALLSFLREMEFNTLTRRIAEKLGVELPPMPEKPAATKRP
                     AEKAQSDPPALAKKADTTALPAGAGTPAAVVAFAEEASVTPAIDRSKYETVTTIERLQ
                     QWIAEARNVGRFSIDTESTSRDPMQAELVGFSMAIAPGQACYVPLGHRPANDSFDFGD
                     GSAPEQISLDVALAAMKPVLEDPSILKIGHNIKYDWLLLSRYGIEIKSFDDTLLMSYA
                     LDGGRNNHGLDELAARHLKHTTITFDKVIEHAPGPKRNGKTFANVPIDKATEYAAEDA
                     DVTRRLWMRFKPRLVAERMTTVYETLERPLVPVIASMERAGIKVDRNVLSRLSGNFAQ
                     SIARLEEEVYELAGHKFNLGSPRQLGELLFERLKLPGGKRTKSGQWETRANLLDDLVQ
                     NEELPEQARKLVNTMLEWRQLAKLKSTYTDALPTYIHPETGRIHTCYSLAATTTGRLA
                     SSDPNLQNIPVRTKEGRAIRTAFVAEPGMLLVSADYSQIELRVLAHIADIPALKQAFA
                     DGL"
     gene            2903..2979
                     /locus_tag="VFX46_00710"
     tRNA            2903..2979
                     /locus_tag="VFX46_00710"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2937..2939,aa:Arg,seq:ccg)
     gene            3109..>3413
                     /locus_tag="VFX46_00715"
     CDS             3109..>3413
                     /locus_tag="VFX46_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599137.1"
                     /translation="MARTPTPCRDSFDVVALALKGIKDGIWPAAKERVRLAAARDRRL
                     TAADQAVLSVYMAKNRRSTGDVRLTIAELAAAAGDVEPTIRRALKHLIEAGCVLPRQ"
BASE COUNT          550 a         1071 c         1090 g          702 t
ORIGIN      
        1 agtccgtccg cgaacgcctg cttgagcgcc ggaatatctg cgatgtgcgc cagtacgcga
       61 agttcgattt ggctgtagtc ggcagaaacc aacaacatgc ccggctcggc gacgaaggcc
      121 gtgcgaatgg cacgcccttc cttcgtgcgg acgggaatgt tctgcaggtt gggatcgctc
      181 gaggcgagcc ggcccgtcgt cgtcgcagct agcgaatagc aggtatggat gcgccccgtc
      241 tccggatgga tatacgtcgg cagcgcatcc gtataggtcg acttgagctt ggccagctgg
      301 cgccattcca gcatggtgtt gacgagtttg cgcgcctgct ctggtagctc ctcgttctga
      361 acgaggtcgt cgagcaggtt ggcgcgcgtc tcccactggc cggacttggt acgcttgccg
      421 cccggtagct tcagccgctc gaacaacagc tcaccgagct ggcgcggtga acccaggttg
      481 aatttgtggc cggcgagctc gtagacctcc tcctcgagcc gcgcgatgct ctgggcgaaa
      541 ttgccgctca gccgcgagag cacattgcgg tcgaccttga tgcccgcgcg ctccatgctg
      601 gcgatcaccg gcaccagcgg ccgctccagc gtctcgtaga cagtcgtcat ccgctccgcc
      661 acaaggcgcg gtttgaagcg catccagagc cgccgcgtca cgtccgcgtc ctcggcggcg
      721 tactccgtcg ccttgtcgat cggcacgttc gcgaaggtct tgccgttccg cttcggcccc
      781 ggtgcgtgtt caatcacctt gtcgaacgtg atcgtcgtgt gcttcaggtg gcgcgctgcg
      841 agttcatcga ggccgtgatt gttgcgcccg ccgtcgaggg cgtaggacat caatagcgtg
      901 tcgtcgaacg acttgatctc gatgccgtat cggctgagca gcagccagtc gtacttgatg
      961 ttgtggccaa tcttgaggat cgacgggtct tcgagcaccg gcttcatggc ggccagtgca
     1021 acgtccagtg aaatctgctc aggcgcgctc ccgtcgccga aatcgaagct gtcgttagct
     1081 ggtctatgac ccagcggcac gtagcaggcc tgtccgggcg caatggccat agagaaacca
     1141 accagctccg cctgcatcgg gtcgcgggac gtggattccg tgtcgatgct gaaacggccc
     1201 acgttgcgcg cttcggcgat ccactgctgc agcctctcta tcgtcgtcac cgtctcgtat
     1261 ttcgagcggt cgatggcagg cgtaacggag gcttcctcgg cgaatgccac tacggccgca
     1321 ggcgtgccgg ccccggccgg cagtgccgta gtgtccgctt tcttggccag agcaggtgga
     1381 tctgattggg ctttttctgc cggccgctta gtcgccgcgg gcttctccgg catcggcggt
     1441 agctcgaccc cgagcttctc ggcgatgcgc cgcgtcagcg tgttgaactc catctcgcgg
     1501 aggaagctga gcagggcatt ggggtcggga tcctgtaccc ccagacgctc gatgggcacc
     1561 ttgaccggaa catcgcactt caagcgaacc agctcccgcg agatccgtgc ctgctctgcg
     1621 aactcgatca gtcgctcccg ccgcttcggt tgcttgatct cgtgtgcgcg ctcgagcagc
     1681 gtgtcgagat cgccgaactc gcggatcagc tcggcagctg ttttgacgcc gattccaggt
     1741 acaccgggca cattgtcagt ggcgtcgccc gagagggctt gcacgtccac cacctgctcc
     1801 ggctcgacgc cgaaccgctc cagcacctcc tcgcggccga tgcgcttgtt cttcatcgtg
     1861 tccagcatcg tgatgccggg ctggatgagc tgcatcaggt ccttgtccga ggatacgatg
     1921 gtgacatcgc cacccttcct aagggcgtca caggcgtacg tggcgatcag gtcatcggcc
     1981 tcgtagccgt cctgctcgat gcaggcgacg ttgaaggctt tgaccgcgcg gcggatcagc
     2041 ccgaattgcg ggacaagctc ctcgggcggg ggcggccggt tcgtcttgta ggcctcgtag
     2101 atgcccgtgc ggaacgtgtc gcgtgccgtg tcgaatatga cggcgatatg ggtcggccgg
     2161 tccgaaagct tgctgtcgcc gagcagcttc cacagcatgt tgcagaagcc gtagacggcg
     2221 ccgacgggca ggccgtcgga cgggcgcgtc agcggcggca atgcgtggaa cgcacggaaa
     2281 atgtagccgg acccgtcgat caggtagacg tggctaccct tgacgatggg caccggctcc
     2341 ccctcgggaa cggtgcactc ggcgacccgt tcgacggact gtggagcggt ctctagcgtc
     2401 atggccggca ctatacgcgg tcccatctgc cgggtcagca gcctggcgcg ggaccgtcgt
     2461 cgcaggcggc agtttggctg cgtatgtctg ggttaggttc cggaggcact acaacatatt
     2521 cattctgcag ctcgagttcg agcccgaggc atgatggctg gccattttgg cctagccgcg
     2581 gccccagccg ttccgcgttt ggccatccct gcggatatgt tcgaccagct tgacttctca
     2641 gcaggtggtc ccgactccgc ggccccagtg tttcattctg cggccccggt gtttcgcgct
     2701 ccgccacccg gcagacttgt tcgacccgct tgccatatcg atccttgggc cctatgtctc
     2761 gctggcgaca atgatgatgg cagcggccgg tgcgtcggaa gtcgcggtcg tatcgcgtgg
     2821 tatcgcttgg gaggccctga cccgacctgg cccaagcctc gattgccgga ttgcgccaga
     2881 tccgttattg tcccagcgtc aagcacccat agctcagctg gatagagcgc tgccctccga
     2941 aggcagaggt cagaggttca aatcctcttg ggtgcgccag ctttttcagt tacttgccgg
     3001 ccgcctcttc tgctcgatgg agcgcgctgt ccgcggaatg cgttcgtagt cggtttcctg
     3061 ctcgttcgtg aaacaatcca actggcgcct gacctggggc gaccgagagt ggcgcgtaca
     3121 ccaacgcctt gtcgcgatag ctttgacgtc gttgcattgg cactcaaggg tatcaaggac
     3181 ggcatctggc cagccgcgaa ggaaagggtg cgcctggccg cggcccggga tcgtcgcctg
     3241 acagcagccg atcaggcggt gcttagtgtc tacatggcga aaaacaggcg ctcgactggc
     3301 gatgtccgct tgacgatcgc ggaattggca gctgccgcag gagacgtgga gcccaccatc
     3361 cgacgggctc taaaacacct gatcgaggca ggttgtgtgc tccctcgtca gaa
//
LOCUS       DASUMG010000042         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1049375, whole genome shotgun sequence.
ACCESSION   DASUMG010000042 DASUMG010000000
VERSION     DASUMG010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049375"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="VFX46_00720"
     CDS             <1..760
                     /locus_tag="VFX46_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX5599138.1"
                     /translation="PRPAAPPAMSDEQILALYEKGTYELVPHDNMRRIIAERLTLAKQ
                     TIPHFYLSIDCRIDALLEARERINASAPKEGPRAFKLSVNDFVIKAWALALQQVPAAN
                     ATWTEQGTLRHKYSDIGVAVAIEGGLFTPVVRHAEMKSLSEISNEMRDLAERARKRRL
                     APHEYQGGTTSISNLGMYGIRAFDAVINPPHATILAVGMGEKRPIVVGDRIEIATMMS
                     CTLSCDHRVVDGALGAELLAAFKALIEDPVRMLV"
     gene            1009..1446
                     /locus_tag="VFX46_00725"
     CDS             1009..1446
                     /locus_tag="VFX46_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599139.1"
                     /translation="MFKTFRGPASLLLGAVLLAGCSGDGALLGSPITTGSIQPAIDPA
                     CVKLTAEIDGLMKEGVAEKVEKAAANKYRLKKADLVKADRLNKANAEFQNRCALNPTR
                     SAETSAEAKSTEGSGQDGANGIAAAATKSAEAAKAAGSETKTQ"
     gene            1738..>2260
                     /locus_tag="VFX46_00730"
     CDS             1738..>2260
                     /locus_tag="VFX46_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599140.1"
                     /translation="MTMHEDDRRVRSSTTHTAPARTTSVESTRETEPTSDLMVAPAGW
                     RLSWGAVFAGVIVALAAHLVLNLLGIGIGLAGARTGAGMEWLGTGFGIWWTIAGIIAA
                     AIGGWIAGRTLGSADRSDGMIHGLLSWAGATLVIAFLLTTAAGGILGAGMQQQAMGPL
                     GEITGQAGGAPQQQ"
BASE COUNT          432 a          670 c          737 g          421 t
ORIGIN      
        1 accgcggcca gccgcacccc ccgccatgtc ggatgagcag atcctggcgc tctacgaaaa
       61 gggcacctac gagctggtgc ctcacgacaa catgcgcagg atcatcgccg agcgattgac
      121 actggcgaag cagacgattc cgcactttta tctgagcatc gattgccgga tcgatgcatt
      181 gctcgaggct cgtgagcgta tcaacgcctc tgcgccgaaa gaggggccgc gggcgttcaa
      241 actgtccgtt aacgatttcg tcatcaaggc gtgggctttg gcactgcagc aggtcccggc
      301 ggccaacgcg acctggacgg agcagggcac gcttcgtcac aagtactccg acattggcgt
      361 ggcggtggcg atcgagggtg ggcttttcac gcctgtcgtg cggcacgcag agatgaaatc
      421 gctgtccgag atttccaatg agatgcggga tctcgccgag cgtgcccgca aacgccggct
      481 cgcgccgcac gagtaccagg gtggcaccac gtccatctcg aacctgggca tgtacggcat
      541 cagggctttc gatgcggtga tcaacccacc gcatgcaacc atcttggccg tcggcatggg
      601 cgagaagcgg ccgatcgtgg tcggcgaccg catcgagatc gcgaccatga tgagctgcac
      661 gctgtcgtgc gatcaccgcg tcgtcgacgg ggccctaggc gctgagctgc ttgccgcgtt
      721 caaggcactc atcgaagacc cagtccgcat gctggtgtga ttcgggggca attggggaat
      781 ggcgagcgag agttcgccat ttcgtgcctt gtatgcctgc cgaagtgtgc cccaggtgac
      841 acttgcgcct gattctccac agcgatcccc tgatcgcgcc aacgacatct gtcggtaacc
      901 tgggtagtcc gcttgccgca tttctagctg ccacctaagc aggggcagcg gggatcgatt
      961 ccggtgcgct gcgttaaagc aaacggactc acaacggggt acttacaaat gttcaagaca
     1021 tttcgcgggc cggcatcctt gttgctcggg gccgtcctgc ttgctggctg ctcgggtgac
     1081 ggcgcgcttc tgggcagccc gatcaccact ggctctatac aaccagccat cgacccggct
     1141 tgcgtcaagc tgacggctga gatcgacggc ctcatgaagg agggcgtggc cgagaaggtc
     1201 gagaaggctg ccgcgaacaa gtaccgtttg aagaaggccg acctggtcaa ggccgacagg
     1261 ctcaacaaag ccaatgccga attccagaat cgctgcgcct tgaacccgac acggtcggca
     1321 gagacgtcgg ctgaggcgaa gtccacggaa ggttccggtc aagacggcgc caatggcata
     1381 gccgctgcgg caaccaaatc cgcggaagcc gccaaggctg cgggatccga aacgaagacc
     1441 cagtaattcg ccgactggac cgagtgtgaa gatgggcggc gtgtagccgc ccgctttttt
     1501 ataaccacgg ctctgaggag tcgtggaagt catgacccgt gaaccggcgc ggcaattgcg
     1561 tctgggcgtg gtaggcccgc tccgggatcg gctggcaggc aagcgcctga taactcagga
     1621 gaagacagct cgcggacagt gcgacgacgt gcctgatgtc gcacctacag ccctggaact
     1681 tgtgcggaac gccgagttta tccggtgcgt tgtaggtcgt gacattggag gcgcatcatg
     1741 actatgcatg aagacgatcg gcgggtccgc agctccacga cgcatacagc gcccgctcgg
     1801 acaacttcgg tcgaatccac tcgcgagacc gagccgacat ccgatctcat ggttgcacca
     1861 gccggctggc gcctgtcatg gggcgctgtg tttgcgggcg tcatcgtagc tctcgctgcg
     1921 cacctggttc tcaatctcct ggggatcggc ataggccttg cgggtgcgcg gaccggcgcg
     1981 ggaatggagt ggcttggtac cgggtttggc atttggtgga caatcgcggg catcatcgcg
     2041 gctgcaatcg gcggttggat tgccgggcga acgcttggct cggctgatcg cagcgacggc
     2101 atgattcacg ggctcttgtc ctgggcaggc gccacgttgg tcatagcatt cctgctgacg
     2161 accgcagcgg gtggcatcct tggcgcgggc atgcagcagc aggcgatggg tccgcttggt
     2221 gagattaccg gtcaggccgg tggtgccccg caacaacagc
//
LOCUS       DASUMG010000043         2907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_728342, whole genome shotgun sequence.
ACCESSION   DASUMG010000043 DASUMG010000000
VERSION     DASUMG010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728342"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="topA"
                     /locus_tag="VFX46_00735"
     CDS             complement(<1..1129)
                     /gene="topA"
                     /locus_tag="VFX46_00735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX5599141.1"
                     /translation="MNVVIVESAAKAKTINKYLGSGYKVIPSYGHVRDLPSKDGSVEP
                     DRDFAMHWEVDSKANKVMREIADAVKKADKVILATDPDREGEAISWHILQLLDQKRAL
                     KKGVPVERVTFNAVTRDAVLAAMKNPRAIDAPLVEAYLARRALDYLVGFTLSPVLWRK
                     LPGARSAGRVQSVALRLVCDREAEIEAFKSEEYWTVEALLATDKGDEVRSRLVAIDGR
                     QLKKLDIKDAASANAIKAAIEGGRFTVSSVEKKAVRRNPAPPFTTSTLQQEASRKLGF
                     SANHTMQVAQRLYEGVDIGGELVGLITYMRTDGVQIVPEAISEIRRVIEAEYGKRYTP
                     FAREYKSKAKNAQEAHEAIRPTDPSRRPDQVARYLEKDQAAL"
     gene            complement(1354..2472)
                     /gene="dprA"
                     /locus_tag="VFX46_00740"
     CDS             complement(1354..2472)
                     /gene="dprA"
                     /locus_tag="VFX46_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX5599142.1"
                     /translation="MDNSQRLACLRLIRSENVGPITFRALINHFGGAQAALDALPELS
                     RRGGRRTIRICPRDAAEAELEAAERIGARPIFTIEPGYPASLAAIEAAPPLIYAKGDE
                     QLLARPAIAIVGSRLASAAGQQLTRKLAAGLGEAGYVVVSGLALGIDTAAHRAALETG
                     TIAVLAGGLDITYPPQNADLQRSIGEHGCLVSEQPPGFQPRGSDFPRRNRIISGLSLG
                     VVVVEAARRSGSLITARRAADQGREVFAVPGHPLDPRAEGTNQLIKEGATLVTCAEDV
                     VNALQPFGRQPAPRPQPFREPASVLDTAPEPLPQVAGDDRARITAALSPAPVPIDELA
                     RATDLPIRIVQTVLLELALAGRIQHHGSQLVSLIGAPD"
     gene            complement(2525..>2907)
                     /locus_tag="VFX46_00745"
     CDS             complement(2525..>2907)
                     /locus_tag="VFX46_00745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX5599143.1"
                     /translation="LIGSLIGGPEVAMAGGLGAFLGHIFPVWLGFKGGKGVATYIGVL
                     LGLHWPAALAFCIVWLTVAILTRYSSLSALAASVATPLLLWITGKGSLAALAGVLTVL
                     LAIKHTANIQRLIAGTESKIGARA"
BASE COUNT          511 a          918 c          961 g          517 t
ORIGIN      
        1 aaagggccgc ctgatccttt tccaggtagc gtgcgacctg gtccggacgg cgggagggat
       61 ccgtggggcg gatcgcctcg tgggcctcct gagcgttctt ggccttgctc ttgtattcgc
      121 gcgcgaacgg cgtgtagcgc ttgccgtact ccgcctcgat cacgcggcgg atctcggaaa
      181 tggcctcggg cacgatctgc acgccgtcgg tacgcatata ggtgatgagg cccaccagct
      241 cgccgccaat gtcgacgcct tcgtagaggc gctgagccac ttgcatggtg tgattggcgg
      301 agaagccgag ctttcgcgac gcttcctgct gcaaggtcga cgtggtgaag gggggcgctg
      361 gattgcggcg caccgccttc ttctccacgg aagagacggt gaaccgccca ccttcgatcg
      421 ccgccttgat ggcattggcg cttgcggcgt ccttgatgtc gagcttcttg agctgccgcc
      481 cgtcgatagc caccagccgg gagcgcacct catcgccttt atcggtggcg agcagcgcct
      541 cgaccgtcca gtattcctcg gacttgaacg cctcgatctc ggcttcacgg tcgcagacga
      601 ggcgcaaggc cacggactgc acgcgcccag cagagcgggc accgggcaat ttccgccaca
      661 gcaccggcga cagcgtaaac cctaccaggt agtcgagggc acggcgggcc agataggcct
      721 ccaccagtgg agcatcgatg gcccgcgggt tcttcatggc tgcgagcacg gcgtcccggg
      781 tgacggcatt gaaggtgacg cgctcgacgg gcacgccctt cttcagcgct cgcttctgat
      841 cgaggagttg caggatgtgc caggaaatgg cttcgccttc ccggtcaggg tcggtcgcga
      901 ggatgacctt gtcggccttt ttcaccgcat cggcgatctc gcgcatgacc ttgttggcct
      961 tcgagtcgac ctcccagtgc attgcgaaat cgcgatcggg ctcgaccgac ccgtctttgg
     1021 agggcagatc gcgcacgtgt ccgtaggacg ggataacctt gtatcccgat ccgagatatt
     1081 tgttaattgt cttggctttc gcagccgatt ccacaatgac gacgttcatg cgcttccggg
     1141 gtcccacccc tgcggccgtt gcagtttgcc cgcagcaggt cgtgcggggc cgctttagat
     1201 ctgtccctca cgttcagctg ggggaaaatg ggtgatggtt ggtgcgaagt caaataggca
     1261 gaatctgcga gccatgcaga ttggccacat gatacagcga agctcggcgc cagatcgacg
     1321 caggtatcga cccatcacgg ctacaagccc accctagtca ggagctccaa tcagcgagac
     1381 caactggctg ccgtgatgct ggatgcggcc tgccagtgcc agctccagga gcaccgtctg
     1441 cacgatgcgg atcggcagat cggtagcacg ggcaagctcg tcaatgggca ccggggcagg
     1501 gctcagggcg gccgtgatgc gagcccggtc gtccccggcg acctgtggca acggttccgg
     1561 tgcggtatcg agcaccgatg ctggctcacg gaagggctgc ggtctgggag cgggttgtcg
     1621 cccgaaaggc tgcagcgcgt tcacgacgtc ctcggcgcag gtgacgagcg tggccccctc
     1681 cttgatcagc tggttggtgc cctctgcgcg cgggtcgagc ggatggcccg ggacggcgaa
     1741 gacctcgcga ccctggtcgg cagcgcgtcg cgccgtgatg agcgatcccg atcgccgcgc
     1801 tgcttctaca actacgactc ctagcgacag gccggagatg atgcggttgc gccgcgggaa
     1861 gtcgctacct cgtggctgga acccgggcgg ctgctcgctg acaaggcatc cgtgctcgcc
     1921 aatggatcgt tgcaagtcag cattctgagg ggggtaggtg atgtcgagcc ctccggccag
     1981 gaccgcaatc gtccccgttt ccagagccgc gcgatgagcg gctgtgtcga tgcccagggc
     2041 gagccccgac accacgacat agccggcctc tcccaggccg gccgcgagct tccgcgtgag
     2101 ctgctggccc gctgcggaag ccaaccgcga tcccacgatc gcaatagcgg ggcgggctaa
     2161 gagctgctcg tctcctttgg catagatgag gggcggcgct gcctcgatgg cggctagcga
     2221 tgccgggtag ccgggctcaa tcgtgaagat cggccgagcg ccgatgcgct ccgcagcctc
     2281 aagctccgct tctgccgcgt cacgagggca gatgcggatg gtccgccgcc cgccgcgccg
     2341 ggaaagctcc ggcagagcat cgagcgcagc ttgcgccccg ccgaagtggt tgatgagagc
     2401 acggaaagtg atcggcccta cgttctcaga gcggatcagg cgcagacaag cgaggcgctg
     2461 gctattatcc aatcgagcgg tcggcagcgg tgcagacttg gatcgcgcac cttccgctcg
     2521 ggtatcacgc ccgtgcaccg atcttgctct ccgtgccggc aatcaggcgc tggatgttgg
     2581 cggtgtgctt gatggcaagc agaacggtca gtacccctgc cagggcagca agcgagcctt
     2641 taccggtaat ccaaagaagc agaggcgttg ccacgctggc cgcaagagcc gacagggatg
     2701 agtaccgcgt caggatcgcg accgtgagcc agacgataca gaaggccagc gccgccggcc
     2761 aatgcaaccc taaaaggacg cctatgtagg tggccacgcc cttgccgccc ttgaacccca
     2821 gccagaccgg gaagatgtgg cccaggaaag caccgagccc gccagccatg gctacttcgg
     2881 gtccgccaat cagactgccg atgagaa
//
LOCUS       DASUMG010000044         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1049697, whole genome shotgun sequence.
ACCESSION   DASUMG010000044 DASUMG010000000
VERSION     DASUMG010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049697"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VFX46_00750"
     CDS             <1..917
                     /locus_tag="VFX46_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5599144.1"
                     /translation="FVTVNCGAIPENLVESILFGHEKGSFTGAVEKRIGKFQEADGGT
                     LFLDEVGELPLEAQVKLLRALQDGEIDPVGAKRPVKVNIRLISATNRDMIELVKSGRF
                     REDLFYRLNVFPIWVPPLRERLDDVPELAYHFLARFAAEEGKNVTSISEQAMQLLMRY
                     SWPGNVRQLENAIFRAVVLADGPELTVAEFPQIAMHVEGFAPEVPPAPPPKEPKVYTG
                     PAMIGADDQVPRTIELKQSDGAKSIGIPAVTDTGDVRSLVDVEADMIRLALGRYRGHM
                     SEVARRLGIGRSTLYRKMQEYGLNPRPH"
     gene            1086..3140
                     /locus_tag="VFX46_00755"
     CDS             1086..3140
                     /locus_tag="VFX46_00755"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5599145.1"
                     /translation="MRPAWTLLSGLALSAVLTSAAFATAEPERSEAGEVGAPAVAAPV
                     SGATDAVSAEQAEEAPKAEGPATAAVEPRESEAAGHSPEPETDDPATSDTASGFEDPE
                     SQSEPTGLALEPESENPATPEEAVADPGLDTAPEQEAAEAAEPVGDEPAQSEAAELDS
                     GKIVSEQSGTAPEPEAASTSSTAEAAEPLPTVSKFEAEILRHISEAKGLKAEDRDALT
                     AFYQGRGDNLLWIEGGKFNTRAETAIAEIQRADDWGLEASAFELPSAPQSDDPAQVAK
                     AELQLSLAVLKYANHARGGRMDPRQLSNYIDREPPLLPPSDVLAGIARADAADAYLRG
                     LHPKHPQFEKLRQAYLDIKHQRLPVEEAEADVAPDAKARKAKRGNRDTNEAKLRKILH
                     NMEQWRWMPEDLGQFYVWANIPEFTLRVVQNGKMIHSERLIVGKPDTQTPIFSDEMET
                     IVFHPYWAVPNSIKVKEILPGLIRGTNILERNNLRIQYRGRDIDPWSVDWRRTDIRNF
                     HVYQPPGSGNVLGVVKFLFPNKHAVYMHDTPTKHLFARPQRMFSHGCMRVRDPLKFAE
                     ILLAYDKGWDRSQVEWLVRRGPRDNHVKLDKKIPVHITYFTAWVDDDGKLKTVADVYG
                     HEELIQMGLEGKAHLIARKKEDLGAIRAEVVSRLAETSPTWTYPSSSSRQPDWVRRVF
                     GH"
     gene            3430..>3731
                     /locus_tag="VFX46_00760"
     CDS             3430..>3731
                     /locus_tag="VFX46_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX5599146.1"
                     /translation="MSTRKLSLLAGVVVAALVAVHWTARLQAESSQTRTISLYNIHTK
                     ETLTVEYKKDGKYVPAAMEQINWILRDWRKNEVTRMDPELIDLLWEMHAELGSKEP"
BASE COUNT          764 a         1087 c         1182 g          698 t
ORIGIN      
        1 cgttcgtcac ggtgaattgc ggcgcaatcc cggagaatct cgtcgagagc atccttttcg
       61 gtcacgagaa gggatcgttc acgggcgcgg tcgagaaacg catcggcaag ttccaggagg
      121 ccgatggcgg cacactgttc cttgacgagg tgggcgagct gccgctcgaa gcccaggtga
      181 agctcttgcg cgccctccag gatggagaga tcgatcccgt tggtgcgaag cggccggtga
      241 aggtcaacat ccggctgatc tcggcgacca accgcgacat gatcgagctg gtgaaatccg
      301 gccggttccg ggaggacctg ttctaccgcc tcaacgtatt ccccatttgg gtgccgccgc
      361 tgcgtgagcg gctcgatgat gtgcccgagc ttgcctacca tttcctggcg aggttcgcgg
      421 ctgaggaggg caagaacgtc acctcaatca gcgagcaggc gatgcagttg ctcatgcgct
      481 attcctggcc tggaaacgtc cgccagctgg agaacgcgat cttccgtgcc gtggtgctcg
      541 cggacggtcc tgagctgacg gtcgcggagt tcccgcagat cgcgatgcac gtcgaaggct
      601 tcgctccgga agtgccgcct gcgccgcctc ccaaggagcc caaggtttac actggacccg
      661 ccatgatcgg cgccgatgat caggtgccgc gcacgatcga gctgaagcag tcggacggcg
      721 ccaagtcgat cggcataccg gcggtgaccg atacaggcga tgttcgctca ctggtcgatg
      781 tcgaggcgga catgatccgg ctggccctgg gccgctatcg cgggcatatg tctgaagttg
      841 cacgccggct gggaatcggg cgttcgacgc tctaccggaa gatgcaggaa tacgggctga
      901 acccccgtcc tcattgaggt gagagatcac gttttgttta gcatcgcgaa gggccattga
      961 acggagcgtc tgtctttccg gtcacagttc cgacaaaaga agattctcag gaaagatcag
     1021 gcgtgtaact gttgctggct gagccgaagc aaggtacgta cggctcagcc gcatggagga
     1081 tcctgatgcg acctgcttgg accctgctct ccggactggc gctgtccgcc gtcttgacaa
     1141 gtgcagcatt cgcgacggcg gagccggagc gcagcgaagc aggcgaggtc ggcgcgccag
     1201 ctgtggcggc gccggtgagc ggtgcaactg acgcagtttc agctgagcag gctgaagagg
     1261 cgccgaaagc tgaagggccg gctacagcgg cggtcgagcc gcgcgaaagt gaagctgctg
     1321 gccattcacc tgagccggag acggacgacc cggccacgag cgacacggcg agtgggttcg
     1381 aggatcccga gtctcagtcg gagccaacag gcctggcgct cgagcctgag tctgaaaatc
     1441 cggccacgcc ggaggaagcc gtagccgatc caggccttga cacggctcct gagcaggaag
     1501 cagcggaggc cgccgagccg gtcggtgacg agccagctca atcggaggcc gctgaactcg
     1561 actcgggaaa gatcgtatct gagcagtctg gtactgcccc ggagccggag gcggcctcga
     1621 cctcaagcac ggcggaagca gctgagccgc tgccgacggt gagcaagttc gaggctgaaa
     1681 ttctgcggca catctccgag gcgaaaggct tgaaagccga ggaccgtgat gctctgacgg
     1741 ctttctatca aggtcgcggc gataatctgc tctggattga gggcgggaaa ttcaacaccc
     1801 gcgctgaaac ggcgatagct gaaatccagc gcgccgacga ttggggtctc gaagcgtctg
     1861 cattcgagct gccttcggcg cctcagtcag atgatccagc ccaggtcgcg aaagccgagc
     1921 ttcagctgtc gctcgctgtg ctgaagtatg ccaatcacgc acgcggtggg cgcatggatc
     1981 cgcggcagct ttcgaattac atcgatcgcg agccgcctct gctgccccct tctgacgttc
     2041 tagcgggaat tgctcgggcg gatgcggccg atgcctattt gcggggcttg catccaaaac
     2101 atccgcagtt cgagaagctt cgccaagcct acctggacat caagcaccag cggctaccgg
     2161 ttgaggaagc cgaagcggac gttgctcccg atgccaaagc gcgcaaggcg aagcgcggga
     2221 atcgggacac gaacgaagcg aagctgcgca agatcctcca caatatggag caatggcgtt
     2281 ggatgccgga ggatctcggc cagttctacg tttgggcgaa cattcccgag ttcacgcttc
     2341 gggtcgtgca aaatggcaag atgatccatt cggagcgtct gatcgtcggc aagccggata
     2401 cgcaaacgcc gattttctca gacgagatgg agacgatcgt cttccacccg tactgggcgg
     2461 tgccgaacag catcaaggtc aaggagatcc tgccggggtt gatccgcggc accaacattc
     2521 tcgagcggaa taatctgcgg atacagtatc gcggccgcga catcgatcca tggagcgtcg
     2581 actggcggcg gacggatatt cgcaatttcc acgtctacca gccaccgggc tctggcaacg
     2641 tgctcggcgt ggtcaagttc ttgttcccga acaagcacgc cgtgtacatg catgataccc
     2701 cgacaaagca tcttttcgcg aggccgcagc gcatgttcag ccacggctgt atgcgcgtcc
     2761 gcgatccctt gaagttcgcc gaaattctgc tcgcctatga caagggctgg gatcgcagcc
     2821 aagtggaatg gctggtccgg cgtggtccgc gcgacaatca tgtcaaattg gacaagaaga
     2881 ttcccgtcca catcacctat tttaccgcct gggttgatga tgacgggaag ctgaagacgg
     2941 tggccgacgt ctacgggcac gaggagctga tccaaatggg cctggaaggc aaggcgcatc
     3001 tcatcgcccg caagaaggag gatttgggcg ccattcgagc ggaggttgtc agccgtctcg
     3061 ccgagacgtc cccgacctgg acatatcctt cgtcttcttc acgtcagccg gattgggtgc
     3121 gccgcgtgtt cggccactga ggcagtccga gacttcaagt tatcttcact ttggcagccc
     3181 cgtgcgtttc tcgtgcacgg ggctgctgcc atttgggaat ccgatgacaa agtccgcagt
     3241 ctgatggagc taatccctcc gtctgcctct ttatcgcccg actgacgttc taagagacgc
     3301 tttgcgggga gccttaacgg gaggttaaca atccagcgtt agcgctaaag acctgctacg
     3361 gtccgctcca aatcaaagca catcgcggca ctcaaggcag gcagcgttcg gtcaggggtg
     3421 gggctagtct tgtcaacgcg taagctgagt ctcctcgccg gtgtcgtcgt ggcggcactt
     3481 gttgccgttc attggaccgc ccggctccag gcggaatcct cgcagactcg gacgatctcg
     3541 ctctacaaca tccataccaa ggaaacccta acagtcgagt acaagaagga cggcaagtac
     3601 gtcccggccg cgatggagca gatcaactgg atcctgcggg attggcgcaa gaacgaagtc
     3661 acccgcatgg accccgagct catcgatctg ctttgggaga tgcatgcgga gcttggctcc
     3721 aaggagccca t
//
LOCUS       DASUMG010000045         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1130018, whole genome shotgun sequence.
ACCESSION   DASUMG010000045 DASUMG010000000
VERSION     DASUMG010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130018"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1027)
                     /locus_tag="VFX46_00765"
     CDS             complement(212..1027)
                     /locus_tag="VFX46_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX5599147.1"
                     /translation="MRSSGLRVIAIANQKGGVGKTTTAINLGTALAAAGERVLVVDLD
                     PQGNASTGLGIGMEERTLTTYDVMMMRCSLLDAAIKTQVPGLTIVSANSDLIGIEKEL
                     MNEPTRPFRMRDAIGGLIGQQRANPEEAITYVLIDCPPSLNLLTLNAMVAAHAVLVPV
                     QCEFFALEGISQLKENIEQIKATLNPGLEIQGVVLTMHDQRTALSREVAQNVRAFFGA
                     KVYDTVIPRNTRVAEAPSHGKPILLYDVNSAGSQAYMRLAAEIIERERRYKAA"
     gene            complement(1039..1713)
                     /gene="rsmG"
                     /locus_tag="VFX46_00770"
     CDS             complement(1039..1713)
                     /gene="rsmG"
                     /locus_tag="VFX46_00770"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX5599148.1"
                     /translation="MNQRRRFERIDNPESFQRAFGVSRETIYRLSTYESLLRQWQKAV
                     NLVAPGTLSQVWHRHLADSAQLAKLLPAEAKTLLDLGSGAGFPGLVLAMMSVERDGLK
                     VTLLESDSRKTAFLREVARRAGVPVDILTARIENPETQRKVGQVDVVTARALAPLSRL
                     LELAAPYLSPCGVGVFLKGRDVHGEIDEASVGWRFDVELIPSMTDTHGRIAVVRNVTA
                     KRRVEP"
     gene            complement(1710..>2303)
                     /locus_tag="VFX46_00775"
     CDS             complement(1710..>2303)
                     /locus_tag="VFX46_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5599149.1"
                     /translation="TSRAEYRLALRADNADQRLTPLGQEVGCVGRERALAFERKRQAL
                     AEGRQLLERLSLTPNEAARHGLEINRDGRRRTAFELLAYPGITLTRLAAIWPEIGKLD
                     PKIAAQLEVDARYASYLKRQAEDVEALRRDEAVAIPLDFDFSSLPGLSTEVRQKLSLH
                     RPATLAQAARIDGVTPAALMILLSRLKRPAERRREIA"
BASE COUNT          420 a          736 c          683 g          464 t
ORIGIN      
        1 gtcctcctcg ttgaactccc gccgcggatt gagcgcgctg gcccggatct ggtcgatcgg
       61 aaccagcctc tgttcacctt cattcggcag cttcggttcc gcaagaaccg gatcaccaat
      121 gagagatgcg agacccctgc ccagccggct cttctggagt ggagctccca tgacccttcc
      181 gttctcctga tcttattcgg tgcctgcttc ctcaggctgc tttgtagcgc cgctcgcgct
      241 caatgatctc cgcagcgagc cgcatgtatg cctgactgcc ggcactgttc acgtcgtaaa
      301 gcagaatcgg cttgccgtgg gatggcgctt ccgcgactct cgtgttgcgg ggaatcacgg
      361 tgtcatagac cttggcgcca aagaacgcgc gaacgttctg cgcgacctcc ctcgaaagcg
      421 ctgtccgttg atcgtgcatg gtcagcacca cgccctggat ctccagtcca ggattgagcg
      481 tggccttgat ctgctcgatg ttctctttga gctgcgagat accctcgaga gcgaagaact
      541 cgcattgaac aggcacgagc accgcgtgcg cagcgaccat cgcgttgagc gtcagcagat
      601 tgagcgacgg cgggcaatcg atgaggacat acgtgatggc ttcctcgggg ttcgcccgct
      661 gctgaccaat caggccacca atagcgtcgc gcatccggaa cggccgcgtc ggctcgttca
      721 tgagctcctt ttcgatccct atcaggtccg aatttgcaga gacgatcgta agtcccggaa
      781 cctgggtctt gatggccgca tcgagaagac tgcaccgcat catcatgacg tcgtaggtcg
      841 tgagggtgcg ctcctccatg ccaatcccga gcccggtcga tgcgttaccc tgaggatcga
      901 gatccaccac gagcacccgc tcgccggccg cagctagtgc cgtacccagg ttgatggcag
      961 tggtcgtctt gccgacgccg cccttctggt tggcgatggc gatcacccgc agtcccgagg
     1021 atctcatggt cttgccattc aaggctcaac cctccgtttg gctgttacat tgcggaccac
     1081 ggcaattcgg ccatgtgtat cggtcatgct cggtatgagc tcgacgtcga aacgccagcc
     1141 gacacttgcc tcgtcgattt cgccgtggac atcgcgcccc ttcaaaaaca caccgacacc
     1201 gcagggcgat aagtacgggg ccgccaattc gagcaggcgg ctcaacgggg caagagcacg
     1261 cgccgtgacc acatctactt ggcccacttt acgctgagtc tccgggtttt cgatccttgc
     1321 cgtgaggatg tccacaggca ccccggctcg acgggcgacc tcgcgcagaa aagctgtctt
     1381 ccgcgagtcg ctttcgagga gcgtgacctt caatccgtct cgctcgacgg acatcatggc
     1441 cagcactaat cccgggaaac cggcccccga gcccaaatcg agcagcgttt tcgcctccgc
     1501 cggtaggagc ttcgccagct gagcgctatc ggcaagatgg cggtgccaaa cctgcgagag
     1561 tgtcccaggc gctacgaggt tgacggcctt ctgccactgt cgaagcagcg actcataagt
     1621 gctcagccga taaatggttt cacgtgaaac gccgaaagcg cgctggaagc tttccgggtt
     1681 atcgatgcgc tcgaatcgcc gacgttggtt catgcaattt cgcggcgccg ctccgctggc
     1741 cgcttgagac ggctgagcaa gatcatgagg gcggccgggg tcactccatc tatgcgcgcg
     1801 gcctgagcta gggttgctgg acgatgcagg ctgagcttct gccgcacttc ggttgacagc
     1861 cccggcaggc tcgagaaatc gaaatccagc ggaatagcaa ccgcctcgtc tcgccggagg
     1921 gcctcgacgt cctcggcttg gcgtttcagg taggaagcat agcgcgcgtc gacttcgagc
     1981 tgcgccgcga tcttcgggtc cagcttcccg atctcgggcc aaatggctgc aagacgagta
     2041 agcgtaatgc cgggataggc cagcagctcg aaggctgtac gccgccgtcc atcgcggttg
     2101 atttcaaggc catgccgggc cgcttcgttg ggtgtcaggc taagccgctc cagcagttgt
     2161 cggccctcgg ctagcgcttg ccgcttgcgc tcgaaggcaa gcgcgcgctc gcggccaacg
     2221 catccgacct cctgacccaa gggcgtcaga cgttgatccg cattgtcggc ccgaagcgcg
     2281 agacgatact ccgcccggga tgt
//
LOCUS       DASUMG010000046         2176 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_6199, whole genome shotgun sequence.
ACCESSION   DASUMG010000046 DASUMG010000000
VERSION     DASUMG010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6199"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFX46_00780"
     CDS             complement(<1..129)
                     /locus_tag="VFX46_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX5599150.1"
                     /translation="MLIGVPKEIKNHEYRVGLIPASVRELVHHGHKVLVESKAGAGI"
     gene            complement(224..1591)
                     /locus_tag="VFX46_00785"
     CDS             complement(224..1591)
                     /locus_tag="VFX46_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007760124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX5599151.1"
                     /translation="MSATAKAVAQSFAPAQFDPTPNNLESFWMPFTANRQFKSKPRLF
                     VAAEGMHYTTIDNRKVLDGTAGLWCCNAGHGRKKISEAVARQIQQMDYAPAFQMGHPS
                     SFELASRLTHLVPGDLDHVFFTNSGSESVDTALKIAIAYHRAKGQGTRQRLIGRERGY
                     HGVGFGGISVGGIVANRKFFGPLLNGVDHLPHTHNLEKNAFSQGQPEWGAHLADDLER
                     IVTLHDASTIAAVIIEPVAGSTGVLIPPKGYLQKIRQICDKHGILLIFDEVITGFGRL
                     GSPFAAEHFGVQPDMITCAKGLTNGAVPMGAVFARKHIYDAFMDNSAENAIELFHGYT
                     YSAHPVACAAGLAVLDIYKEEGLFENCRALSKYWEDGVHSLKGIKSVIDLRNMGLIAG
                     IELESIPGKPTARAFNAFLKAYEKGLLIRTTGDIIALSPPLIVEKSHIDQIFSMLADI
                     LKNLD"
     gene            1756..>2176
                     /locus_tag="VFX46_00790"
     CDS             1756..>2176
                     /locus_tag="VFX46_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5599152.1"
                     /translation="MAEGTGFDLGSRMKNLREAHNLSQRELAKRAGVSNAIISMIEQN
                     RSSPSVGMLKKLLDGFPLSLAEFFALEAPPKPQIYFTAKELVELAGGPVSYRQVGRDM
                     TGKAIQLMHEHYAPGADTGAQMLTHDSEEGGIIVRGRL"
BASE COUNT          391 a          650 c          724 g          411 t
ORIGIN      
        1 gatgcccgcg cccgccttgc tctcgaccag aaccttgtgc ccgtgatgga ccagctcacg
       61 caccgaggcg gggatgagcc ccacccggta ttcgtgattc ttgatctcct tcggcacgcc
      121 aatcagcatg gtaagcactc cctgatctca aagttatggt cggcggagtt gacccccgcc
      181 gaccggttcg ttcagcttcc gtggccgcaa tccgcggccg atgtcaatcc aggttcttca
      241 ggatgtctgc gagcatgctg aagatctggt cgatgtggct cttctccacg atcagcggcg
      301 gcgacagcgc gatgatgtcg ccggtggtgc ggatcaggag gcccttctcg taggccttca
      361 ggaaggcgtt gaaggcgcgc gccgtcggct tgcccgggat cgattccagc tcgatgccgg
      421 cgatcaggcc catgttgcgc aggtcgatga cgctcttgat gcccttcagc gagtgcacgc
      481 catcttccca atacttggag agggcgcggc aattctcgaa caggccttcc tccttgtaga
      541 tgtcgagcac ggcgaggccc gccgcgcacg ccaccggatg ggcggagtag gtgtagccgt
      601 ggaacagctc gatcgcgttc tccgcggagt tgtccatgaa cgcgtcatag atgtgcttgc
      661 gcgcgaacac cgcgcccatc gggaccgcgc cattggtgag gcccttggcg caggtgatca
      721 tgtcgggctg cacgccgaaa tgctcggccg cgaacggcga gccgaggcgg ccaaagccgg
      781 tgatgacctc gtcgaagatc agcaggatgc cgtgcttgtc gcagatctgc cggatcttct
      841 gcagatagcc cttgggcggg atcagcacgc cggtagaacc ggcaaccggc tcgatgatca
      901 ccgcagcgat ggtcgaggca tcatgcagcg tgacgatgcg ctccaggtcg tcggcgaggt
      961 gcgcgcccca ttccggctgg ccctgggaga aggcgttctt ctcgagattg tgcgtgtgcg
     1021 gcaggtggtc gacgccgttg agcagcgggc cgaagaactt gcggttggca acgatgccgc
     1081 cgaccgagat gccgccgaag ccgacgccgt gatagccgcg ctcgcggccg atcagccgct
     1141 ggcgggtgcc ctggcccttg gcgcggtggt aggcgatcgc gatcttgagc gcggtgtcga
     1201 cagattcgga gcccgaattg gtgaagaaga cgtggtcgag gtcgcccggc accaggtgcg
     1261 tcaggcgcga ggccagctcg aacgacgagg gatggcccat ctggaaggcc ggcgcgtagt
     1321 ccatctgctg gatctgcctg gcgaccgctt cggagatctt cttgcggccg tggccggcgt
     1381 tgcagcacca caggcccgcg gtgccatcga gaaccttgcg gttgtcgatc gtcgtgtagt
     1441 gcatgccttc ggcggcgacg aacaggcgtg gcttggactt gaactgccgg ttcgccgtga
     1501 acggcatcca gaaggattcg agattgttgg gggtcgggtc gaactgagcc ggggcaaaag
     1561 actgggcaac cgctttggct gtggcggaca tggggatggt cctcagggcc agtggaagat
     1621 gagcgcacca tagcaatgtt caacaaatcg aacaagagcg cataaatgtt cacaattccg
     1681 gccagtttgg ccttgatcct gggtacagtt gttcattata ttgagcgacc aagccattgg
     1741 gatgctggat ttaacatggc cgagggcacc gggttcgatc tgggcagtcg catgaaaaac
     1801 ctgcgggagg cgcacaacct ctcgcagcgc gagttggcga agcgcgccgg cgtctccaac
     1861 gcgatcattt ccatgatcga gcagaaccgc tcctcgccct cggtcggcat gctgaagaag
     1921 ctgctggacg gtttcccgct ctcgctggcc gagttcttcg cgctcgaagc cccgcctaag
     1981 ccccagatct atttcacggc caaggaactg gtggaactgg ccggcggccc ggtttcctat
     2041 cgccaggtcg gccgagacat gaccggcaag gcgatccagc tcatgcacga gcactatgcg
     2101 cccggcgccg atactggtgc gcagatgctg acccacgatt cagaagaggg cggcatcatc
     2161 gtgcgcggcc ggctgg
//
LOCUS       DASUMG010000047         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_889929, whole genome shotgun sequence.
ACCESSION   DASUMG010000047 DASUMG010000000
VERSION     DASUMG010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889929"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..153
                     /locus_tag="VFX46_00795"
     CDS             <1..153
                     /locus_tag="VFX46_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX5599153.1"
                     /translation="NTSINPARSTGTALFQGGWAIQQLWVFWLVPLIGGVVGGLIHRA
                     LFEEHE"
     gene            complement(203..2122)
                     /locus_tag="VFX46_00800"
     CDS             complement(203..2122)
                     /locus_tag="VFX46_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEX5599154.1"
                     /translation="MAIQQSIKAEEPLKREGNAWASEGHADHWTVLRGLLPYVWPKGR
                     PDLRLRVVLAFAVLVLAKVVTIAVPIFFKEATDLLASSTPGLSLEQGVGLVAGALIVA
                     YGASRVLMLVLNQIRDVFFTRVGQHAVRELNNRTFQHLHRLSLRFHLERRTGGLSRVI
                     ERATRAIELIIRMGILNVVPTALELLLICGVLLYYFDIIYVLIILITVAAYMWFTFAA
                     SEWRIAIRREMNDSDTEANTKAIDSLLNFETVKYFGNEELESRRFDASMARYENAAVR
                     TYYSLGVLNSGQSLIFSLGMTAVMLLTARGILAGENTVGDLVLVNTILIQLYMPLNFM
                     GMVYREIKQGLVDLENMFSLLNEHPEIVDKPGAKPLKVDRGTIRFENVYFSYEPDRPI
                     LKDVSFEVPAGHMVAIVGPSGAGKSTIGRILFRFYDISAGQVTIDGQDIRDVTQKSLR
                     AAIGVVPQDTVLFNDTIFYNIKYGRPDATDEEVYEAARLAQIDDFIRQLPQGYRTMVG
                     ERGLKLSGGEKQRVAIARTILKQPPILLLDEATSALDSHTEKEIQEALDRVSRNRTTL
                     VIAHRLSTIVHADNILVLDKGRLVEQGTHAQLLAKGGLYASMWNRQLRAEKAREELAR
                     SLEEAELGSASEPAV"
BASE COUNT          426 a          733 c          692 g          445 t
ORIGIN      
        1 aatacgtcga taaatcctgc gcgcagcacc ggcacggcgc ttttccaggg tggctgggcg
       61 attcagcagc tgtgggtttt ctggcttgtg cctttgatcg gcggcgttgt cggcggactg
      121 atccaccgcg cgctgtttga agagcacgag tgaagtttca ccgcagtcat tcggcacgat
      181 tggcagagcc ggggtggtct cttcagacgg ccggctctga tgctgagcct agttccgcct
      241 cctccagcga ccgcgccagc tcctcgcgcg ctttctctgc tcgcagctgc cggttccaca
      301 tgctggcgta gaggccaccc ttcgcgagga gctgcgcatg cgtgccctgc tccacgaggc
      361 gtcccttatc gagcaccaga atgttgtccg cgtgaacgat ggtcgacagg cggtgcgcaa
      421 tgacgagcgt cgtccgattg cgagacaccc ggtcgagcgc ttcctggatc tccttctccg
      481 tatggctgtc gagcgcgctg gttgcctcgt caagcagcag gatcggcggc tgcttgagga
      541 tggtacgggc gattgcaaca cgctgcttct cgccgcccga cagcttcagc ccgcgctcgc
      601 cgaccatcgt gcggtagccc tgcggaagct ggcggatgaa atcatcgatc tgcgccagtc
      661 gcgccgcctc gtagacctcc tcgtcggtgg cgtccggtcg gccatacttg atgttgtaga
      721 agatcgtatc gttgaagagc accgtatctt gcggcaccac gccgatcgct gcacgcaagc
      781 tcttctgggt cacatcgcgg atatcctgcc cgtcgatcgt cacctggccg gcggagatat
      841 cgtagaagcg gaacaggata cgccctatcg tcgacttccc ggccccggaa ggtccgacga
      901 tggccaccat gtgcccggca ggcacctcga acgagacatc cttcaaaatc ggccgatccg
      961 gctcataaga gaaataaaca ttctcaaatc ggatcgtccc gcgatccacc ttgagaggct
     1021 tcgcacctgg cttgtcgacg atctccggat gctcgttgag caatgagaac atgttttcca
     1081 gatcgacgag accctgtttg atctcgcgat acaccatgcc catgaagttc agcggcatgt
     1141 agagctggat cagaatcgtg ttgaccagca cgaggtcgcc gaccgtgttc tcgccggcta
     1201 agattccgcg tgcggtgagc agcatcaccg ccgtcatgcc caacgagaag atcagcgatt
     1261 ggcctgagtt gagcacgccc agcgagtagt acgtccgcac tgcggcgttc tcgtatctcg
     1321 ccatcgaagc gtcgaaccgg cgcgattcga gctcctcgtt gccgaagtac ttgaccgtct
     1381 cgaagttgag caggctgtcg atggccttcg tgttggcctc ggtatcgctg tcgttcatct
     1441 cccgccggat cgcgatccgc cattcgctag ccgcgaacgt gaaccacatg tacgcggcga
     1501 cggtgatcag gatgatcagc acatagatga tgtcgaagta gtacagcagc acgccgcaga
     1561 tgagcagtag ctcaagcgcg gtgggcacga cgttgagaat gcccatccgg atgatgagct
     1621 ctattgcgcg cgtcgcgcgc tcgatgacac ggctcaagcc acccgtgcgc cgctcgaggt
     1681 gaaagcgcag cgagaggcgg tggagatgct ggaaggtgcg gttgttgagc tcacgcaccg
     1741 catgctggcc caccctcgtg aagaacacgt cccggatctg gttcaagacg agcatcagga
     1801 cgcgcgaggc gccgtaggca acgatcaacg cccctgcgac gagcccgact ccctgctcga
     1861 ggctcagacc cggcgtgctc gaggcaagca gatcagtggc ctccttgaag aagatgggga
     1921 ctgcgatcgt caccaccttc gccagaacga gaacggcgaa ggccagcacc accctcaggc
     1981 gcaggtctgg ccgccccttc ggccatacgt agggcagcaa tccccggagc actgtccaat
     2041 ggtccgcgtg accctctgac gcccacgcat ttccttcccg cttgagcggc tcttccgcct
     2101 tgatcgactg ctgaattgcc atgcgtcttc tcttttcgcc ctggagcagc cttcgccgaa
     2161 cggctcaacg cccaacggac cgcacgctcg gggggtgccc ctgggctctg cccagcaaga
     2221 ttccgggcgt caaagcggcg gccgggacgg ccgccagaca gatgctggct cgccccggcc
     2281 gccaaacccg ggaggt
//
LOCUS       DASUMG010000048         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_6320, whole genome shotgun sequence.
ACCESSION   DASUMG010000048 DASUMG010000000
VERSION     DASUMG010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6320"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /gene="acnA"
                     /locus_tag="VFX46_00805"
     CDS             complement(<1..1857)
                     /gene="acnA"
                     /locus_tag="VFX46_00805"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5599155.1"
                     /translation="MDSFGTASELQVGGKTYRYFSLAKLKQQGFEIDRLPYSLRILLE
                     NLLRNENGSSVEKADIEALATWKPQAKGQKEIAFTPARVLMQDFTGVPAIVDLAAMRD
                     AMARLGGDPAKINPLQPLELVIDHSIQADVFGRPDAMKLNTELDYARNGERYAFLKWG
                     QQAFRNFKVSPPNGGICHQVNMEYLARVVFGAREGDVPPGTEKPLAYPDTLVGTDSHT
                     PMVNGLGVLGWGVGGIEAEAAMLGQPISMLIPDVIGFRLHGQLREGATATDLVLTVTQ
                     MLREKGVVGRFVEFYGPGVSNVPVTDRATIGNMSPEYGSTCSIFPVDKLTLDYLRFTG
                     RTDEEVALVEAYMKAQGLFHDANAPEPIYTDTLELDLSTVEPSIAGPGRPQDRIPLRM
                     AKKAFEQALPSLLPPEPPPTQKLAAAEVPNQGKVGVWGESSPKDLEQVLPPATDIGKE
                     LRHGSVVIAAITSCTNTSNPSVMIAAGLLAKKAVERGLQRKPWVKTSLAPGSRVVTEY
                     YDKAGLTPYLESLGFNLVGYGCTTCIGNSGPLLPEVSEAIQKNKLVVASVLSGNRNFE
                     GRIHSEVRANYLMSPPLVVAFAIAGRIDIDLESEPVGYDPNGQPVFLRDIWPS"
     gene            complement(2084..>2487)
                     /locus_tag="VFX46_00810"
     CDS             complement(2084..>2487)
                     /locus_tag="VFX46_00810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5599156.1"
                     /translation="LKQMSAYDDPDKIVFITNLWSTPASLIPALFVWQWPPLALWPYL
                     IGLGVCAVLGHIALMRGFASTDASLVFTFEFSRMPFSVLAGYLLFGETIDLWTWVGAL
                     IIFTSAVYVTRREAQIARHRQRVEKQDASDP"
BASE COUNT          485 a          767 c          773 g          462 t
ORIGIN      
        1 gctcggccag atgtcgcgca ggaacacggg ctggccgttc gggtcatacc cgacgggctc
       61 cgactctaag tcgatgtcaa tgcggcccgc gatcgcgaac gcgacgacca gcggcggcga
      121 catcaggtag ttcgcgcgga cctcggagtg aattcggccc tcgaaattgc ggtttcctga
      181 aagcaccgag gcgacgacaa gcttgttctt ctgaatcgcc tccgacacct cgggaagcag
      241 cggcccagag ttgccgatgc aggtggtgca cccatagccg acgagattga aacccagcga
      301 ctccaggtaa ggcgtgaggc ccgccttgtc gtagtactcc gtcacgaccc gcgagccagg
      361 cgccaacgat gtcttgaccc agggcttgcg ctgtaggccg cgctcaaccg ccttcttcgc
      421 gagcaatccc gcggcgatca tcacggatgg attggacgtg ttcgtgcagc tcgtaatcgc
      481 tgcaatcact actgacccgt gccgcagctc tttgccaatg tcggtggccg gtggcagcac
      541 ctgttccagg tctttcgggg atgactcgcc ccaaacgccg accttgcctt ggttaggcac
      601 ctcagccgca gccaatttct gcgtcggcgg cggttcgggt ggcagcaggg aaggcaacgc
      661 ctgttcaaac gccttctttg ccatgcgcaa cggaatgcgg tcctgcggac gccccgggcc
      721 ggcgatgctg ggctccaccg tagacagatc aagctccaat gtgtcggtgt agatcggctc
      781 gggagcgttg gcgtcatgga agaggccttg cgccttcatg taagcctcga cgagcgccac
      841 ctcctcgtcc gtacgcccag tgaagcgcag ataatccagc gtcagcttgt cgactgggaa
      901 aatggagcac gtcgagccgt actcgggcga catgttgccg atcgtggcgc gatcggtcac
      961 tggcacgttg ctgacgccgg gaccgtagaa ttcgacaaag cgtccgacga cccctttctc
     1021 gcgcagcatc tgcgtgaccg tcagcacgag gtccgttgcc gttgcgcctt cgcgcagctg
     1081 gccatgaagg cggaagccga tgacatctgg aataagcatc gagatcggct ggcccagcat
     1141 cgccgcctcg gcctcgatgc cgccgacacc ccagccgagc acgccgaggc cgttgaccat
     1201 gggcgtatgg gagtcggtgc caaccagcgt atccggatag gccagaggtt tctccgttcc
     1261 cggaggcacg tcgccctcgc gcgcgccgaa aacgacgcgc gccaagtatt ccatgttcac
     1321 ctgatggcag atgccgccgt tcggcgggct gaccttgaaa ttgcggaagg cctgctggcc
     1381 ccacttgaga aaagcgtagc gctcgccgtt gcgtgcgtaa tcaagctccg tattgagctt
     1441 cattgcatcc gggcggccaa agacatccgc ctggatcgag tggtcgatga ccagctccag
     1501 cggctgcaac gggttgatct tggccgggtc gccgccgagc cgcgccatgg cgtcccgcat
     1561 ggccgccaag tcgacgatgg ccggcacgcc ggtgaagtcc tgcatcagca cgcgcgccgg
     1621 cgtgaaggcg atctctttct ggccttttgc ctgaggcttc cacgtggcca gcgcttcgat
     1681 atcggccttt tcaacgctcg agccgttctc gttccggagc aagttctcga gaagaattcg
     1741 cagcgagtaa ggaagccgat cgatctcgaa gccttgctgt ttcagcttgg caaggctgaa
     1801 gtagcggtaa gtcttgccac cgacctgaag ctcggatgca gtgccgaaac tgtccatgac
     1861 tccctccatt tcgggccggc tcgcagctcg atccaagtca tgccgcggat gatcagtgtt
     1921 tcatccaagc ggcagcttga cgaatccgta caggcccgcg gccgagcgtt gtttaactag
     1981 gatcaaccgg gcgagggtcc gccggttccc gcattgccaa gttggaagcc ataccaagtg
     2041 ctggcgctct cggcgaacga agcttgattc caccggtcga ggcttagggg tcggaagcgt
     2101 cctgcttttc gacgcgctgc cgatggcggg cgatctgtgc ctctcgccgg gtgacgtaaa
     2161 cggcggaggt gaagatgatg agggcgccga cccaggtcca taggtcgatg gtctcgccga
     2221 aaagcaggta tcctgccaag acggaaaacg gcatgcgcga gaactcgaag gtaaagacca
     2281 gcgatgcatc agtcgatgcg aacccgcgca tcagggcgat atggcccagg accgcacaca
     2341 ccccaagtcc gatgaggtaa ggccacaggg ccaacggcgg ccattgccag acaaacagcg
     2401 ccggaatgag cgaagccggc gtcgaccaca gattggtgat gaagacgatc ttatcggggt
     2461 catcatatgc cgacatctgt ttgagca
//
LOCUS       DASUMG010000049         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_86721, whole genome shotgun sequence.
ACCESSION   DASUMG010000049 DASUMG010000000
VERSION     DASUMG010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86721"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1070)
                     /locus_tag="VFX46_00815"
     CDS             complement(111..1070)
                     /locus_tag="VFX46_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX5599157.1"
                     /translation="MVLPFENNRYLAELLGEYDAHLALIEDRLGIEAHAHGNVVILSG
                     SEAACEIARSVLEQLYARVARGEQVGPGDFDGLIRHGRAQVPGALPNGQAHVTTRRRV
                     IKARTPAQSNYIRALEKTDLVFGLGPAGTGKTYLAVAYAAHCLERGVVDRIILSRPAV
                     EAGERLGFLPGDMREKVDPYLRPLYDALYDVLPPEKVSRDLELGVIEIAPLAFMRGRT
                     LANAYVILDEAQNTTSMQMKMFLTRLGENSKMVITGDPTQIDLPAGQTSGLEEAVRLL
                     EGVEGIEVVRFTAADVVRRDLVARIVEAYDRDQAARAERKRHE"
     gene            complement(1255..2592)
                     /gene="miaB"
                     /locus_tag="VFX46_00820"
     CDS             complement(1255..2592)
                     /gene="miaB"
                     /locus_tag="VFX46_00820"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX5599158.1"
                     /translation="MAEARKVFIKTYGCQMNVYDSERMAEALAHHGYTETDDPAEADM
                     VVLNTCHIREKAAEKVYSELGRLRRVKLARKIQGKDTLVAVAGCVAQAEGAEIIARAP
                     IVDLVVGPQAYHRLPDLARRARLENRQLIDTDFPDEDKFARLPRREGKRPATAFLTVQ
                     EGCDKFCTFCVVPYTRGMEVSRPVAQVYEEAVRLVEQGVKEITLLGQNVNAYHGEGPD
                     GRPWSLAKLLRHLSGIEKLERLRYTTSHPRDMDDELIEAHRELPKLMPYLHLPFQAGS
                     DRILAAMNRKHTAEDYLRTVERIRAARPDIALSTDIIVGFPGETEEEFEQTLELVRKV
                     GFAHAFSFKYSPRPGTPAANMQEQLPEEVKAGRLARLQELLQAQQRQFNASCVGKVLP
                     VLFEKPGRRAGQLVGRSPYLQAVHAEAEPSLIGQILPVEIYDASTNSLAGVVR"
BASE COUNT          499 a          930 c          887 g          576 t
ORIGIN      
        1 aggcgctcca atcaccgtct tcgatgatga tgtcgagctg cagacgtcct ggaggctcag
       61 ggtcatcgtc gccgtcgtcc ggcgtctcag tcctcacggt ttctagttgc tcactcatga
      121 cgcttgcgtt cggctctggc agcctgatcc cgatcatagg cctcgacgat gcgcgcaacg
      181 agatccctcc ggaccacgtc tgcggcagtg aagcggacca cctcgatacc ttcgacgcct
      241 tccagcagcc tgacagcctc ctcaagcccg gaagtctggc ccgcgggcag gtcgatctga
      301 gtcgggtctc ctgtgatcac catcttcgag ttctcaccga ggcgggtgag gaacatcttc
      361 atctgcatgc tggtcgtgtt ctgcgcctcg tccagaatga cgtaggcatt cgccagcgta
      421 cggcctcgca tgaaggcgag gggcgcgatt tcgatgacgc ccagctccaa atctcgcgaa
      481 accttctcgg gcgggagcac gtcgtacagc gcatcgtaaa gagggcgcag gtaggggtcg
      541 accttctcgc gcatgtcacc aggcaggaac ccgaggcgct cgccggcctc aacggcaggc
      601 cgcgagagga tgatccggtc cacgacgccc cgctcaaggc agtgcgcggc gtaggcgacg
      661 gcgagatatg tcttgccggt gccggcgggt cccagcccga aaaccagatc cgttttctcg
      721 agggcgcgga tgtagttgct ctgagcaggt gtcctggcct tgatgacgcg acggcgcgtc
      781 gtgacgtgag cctgcccgtt gggcaacgct cccggcacct gtgcccggcc gtggcggatc
      841 agtccgtcga agtccccagg accgacctgc tcgccacgcg cgacgcgtgc gtagagctgc
      901 tcgagaacgc tgcgcgcgat ctcgcaggct gcctcggacc ctgagaggat gacgacattg
      961 ccgtgcgcat gcgcctcgat gccgaggcga tcttcgatca gagccagatg cgcatcgtac
     1021 tcgccgagca gctcggcaag ataccggttg ttttcaaagg gtaggaccaa acgcttcctg
     1081 gtgacggaag ccttggaggg cttcccggcc ccggaagtgg ctgccccacg atatgtgttc
     1141 aatatgcagt ctctcccgcg cggcgggggt tacgattcgt cccgccgccc aactatacta
     1201 acctatgtag tgctcttgtg acagtcgtcc agttcccaag cgcgaaatcc gtccttagcg
     1261 caccacaccg gccaggctat ttgtgctcgc atcgtagatt tctacgggca ggatttggcc
     1321 gatgaggcta ggctctgcct ccgcgtgaac ggcctgcagg tagggtgagc ggcccacgag
     1381 ctgtccggcc cggcgacccg gcttctcgaa gagcacgggg agcaccttgc cgacgcaact
     1441 cgcgttgaac tgccgttgtt gagcttgaag gagctcctgc aaccttgcga gacgcccagc
     1501 cttgacctcc tccggcagct gctcctgcat gttcgccgca ggcgtgcctg gccgcgggct
     1561 atacttgaat gagaacgcgt gcgcgaagcc cactttccgc accagctcca gcgtctgctc
     1621 gaactcttct tcggtttcgc cagggaagcc gacgatgatg tctgtggaaa gggcgatgtc
     1681 cggccgggcc gcacggatgc gctcgacggt gcgaaggtaa tcctctgccg tatgcttgcg
     1741 gttcatcgcg gccagaatgc ggtccgaccc cgcctggaag ggcaggtgta ggtaaggcat
     1801 cagcttcggc agctcgcgat gggcctcaat aagctcatcg tccatgtcgc gcggatggct
     1861 ggtcgtgtag cgcagccgtt cgagcttctc gatgcccgac aggtgcctca acaactttgc
     1921 cagcgaccac gggcgtccgt ccggcccctc gccatgatag gcgttgacgt tctggcccag
     1981 cagggtgatc tccttgacac cttgctccac gagccgcacg gcctcttcat aaacctgggc
     2041 gacgggccgc gatacctcca tgccgcgggt gtaagggacc acgcagaacg tgcagaactt
     2101 gtcacagccc tcttgcactg tcaggaaggc ggtggcgggg cgcttgcctt cacgcctggg
     2161 caggcgcgcg aacttatcct cgtccggaaa gtccgtgtcg atgagctggc ggttctcgag
     2221 tcgggctcgt cgggcgagat ccgggaggcg gtggtaggcc tgcgggccaa caacaaggtc
     2281 cacgattgga gcgcgggcga tgatctcggc gccctccgcc tgggccacgc aaccggcaac
     2341 cgccaccagg gtgtccttgc cttgaatctt ccgcgcgagc ttcactcgcc gcaagcggcc
     2401 cagctccgag taaaccttct ccgcggcctt ctcccggata tggcaggtgt tgagcaccac
     2461 catgtcggct tcggccgggt cgtcggtctc cgtgtatccg tgatgcgcaa gggcctcggc
     2521 catgcgctcg ctgtcataga cgttcatctg acagccgtaa gtcttgatga agaccttcct
     2581 ggcctctgcc atgcgaggac ctcgcaactc ctattcggcg gatcgcaacc tgcgccgcgt
     2641 caacgatgac tgtccagctc ccgctcaggt tcctgaaacg cattgccgca agcggcagcc
     2701 ggtttgctgc attgcaacaa tatttttgag tggatgtcca gattcttggc tgcttgcctg
     2761 tcaagcaccc caaatatgag gtggcctagc gatcgatgcc agcaatcgcc tgtggtgcga
     2821 cctctggctc cacccgcgac ttggccgtgc gctcgaatag cctgtcatca atgttctgtc
     2881 cacgcagtaa ac
//
LOCUS       DASUMG010000050         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_970579, whole genome shotgun sequence.
ACCESSION   DASUMG010000050 DASUMG010000000
VERSION     DASUMG010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970579"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFX46_00825"
     CDS             <1..769
                     /locus_tag="VFX46_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX5599159.1"
                     /translation="SEMITDMRHEVIEELVKRHIPENAYAEQWDAEGLQKAVKDIFGL
                     DLPIVEWAAEEGIADEEILERLIKAADEKAEKKAAEIGPEVFRQIEKMVLLQTLDHLW
                     REHLVTLEHLRQVIGFRAYGQRDPLNEYKAEAFVLFQSMLAKLREAVTGQLMHVELAS
                     PEDRSLLEPVELPPMEAHHIDPTTGLDEFALADAALAAEEMAYANGPDRRVPLQTRRS
                     ADQIDPKNPATWGRVARNAPCPCGSGKKYKHCHGRHH"
     gene            811..>2508
                     /locus_tag="VFX46_00830"
     CDS             811..>2508
                     /locus_tag="VFX46_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5599160.1"
                     /translation="MRILGLVGATHDSGVALLKDGVPELVLEEERFNRDKRTKKFPRR
                     SLALALDHLPGGLDDVDVITTPWDVSRLRRTFCRAVLGRFPLSLSLVLPSAHEPQNNE
                     IVLLNRWLKRRIQKQVGVRDLPPLVNVSHHDAHAAIFFVSPFEEAVVLVMDGFGDDAS
                     TSAYIGRGNRLERLWHTEIFNSIGIVYTFVTKYLGFGFSDEGKVMALAAYGEDTYVEK
                     FRDVIRPLPGGRYQVNFDYFSYDAFGELRPFKQRFFDTFGPPRQPGEELNDRHRDIAR
                     ALQVVTEEIVLHMARELRRSTKIKNLCLSGGVALNCVANARVLAEAGFERVWVPPCAS
                     DTGAPLGSALWHYHQNLGHPRRMELTHPFYGLCYSDHEIEGALAEAGLTGERLSEAEL
                     LRRVAQDLADGKIVGWFQGRSEMGPRALGNRSILADPRRPEMKDIINARVKHREPFRP
                     FAPAVLLDHASEFFHLDQPDPFMTLAPRVRAEKAHLIPAAVHVDGTGRIQTVQRSANP
                     LYYGVIEAFGKLTGVPVLLNTSFNRQEPIVETPRHAVSCYLRTGLDVLVLGAYYTTDR
                     "
BASE COUNT          478 a          749 c          806 g          475 t
ORIGIN      
        1 cagcgaaatg atcaccgaca tgcgtcatga ggtgatcgag gagctggtga agcggcacat
       61 ccctgaaaat gcgtacgcgg aacagtggga tgcggaaggc ctccagaagg cggtgaagga
      121 cattttcggc ctcgacctgc cgatcgttga gtgggcggct gaggaaggca tcgccgatga
      181 agagatcttg gagcgtctga tcaaggcggc cgatgagaag gcggagaaga aggcggccga
      241 gatcggcccc gaggtgttcc gccagatcga gaagatggtg ctcctccaga cgctcgatca
      301 tctgtggcgc gagcatctgg tgacactgga gcacttgcgg caagtcatcg gcttccgcgc
      361 ctatgggcag cgggacccgc tcaacgagta caaggccgag gcgttcgtgc tcttccagtc
      421 catgctcgcc aagctacgcg aagctgtgac cggacagttg atgcacgtcg agctggcctc
      481 gccggaggat cgatctctgc tcgagccggt cgagcttccg cccatggagg cccaccacat
      541 cgatccaaca actggcctcg acgagttcgc gttggcggac gccgctctcg cggccgagga
      601 aatggcgtat gcgaatggtc cggaccgccg cgttcccttg caaacgcgcc gcagtgccga
      661 ccaaatcgat ccgaagaatc cggcaacgtg gggcagagtc gcgcgcaatg caccctgtcc
      721 ctgcgggtct gggaagaagt ataagcactg ccacgggcgg caccactgaa gctaagccgc
      781 tcctccaacc tgctgccgtg gtgaggtcag gtgcggatac tcggattggt cggtgccacc
      841 cacgacagcg gagtggcact tcttaaggat ggcgttcccg agctggtcct cgaggaggag
      901 cggttcaatc gcgacaagcg gacgaagaag tttccccgcc gctcactcgc attggcgttg
      961 gaccacttgc ccggggggct cgacgacgtc gacgtcatca cgacgccgtg ggatgtctcg
     1021 cgtctgcggc ggacattctg ccgggcggtt cttggccggt tccctttgag cttgagtctt
     1081 gtcctgccat cggcgcacga gccgcagaac aacgagatcg tcctcctgaa caggtggctg
     1141 aaacggcgaa tacagaaaca ggtcggcgtg cgcgatctac cgcctctcgt gaatgtcagc
     1201 caccatgacg cgcacgcagc catcttcttc gtcagcccgt ttgaggaggc tgtggtgctc
     1261 gtcatggatg gcttcggcga tgatgcctcc acgagtgcct acatcggccg cggcaaccgg
     1321 ctggagcggc tctggcacac ggaaatcttc aactccatag gcatcgttta cacgttcgtg
     1381 accaagtacc tcgggttcgg gttttcagac gagggcaagg tgatggccct cgccgcgtat
     1441 ggtgaggaca cctacgtcga gaaattccga gatgtcattc ggcccttgcc cgggggccgc
     1501 taccaggtga acttcgacta cttcagctac gatgccttcg gagagctgag gcccttcaag
     1561 cagcgcttct tcgatacctt tgggccgccg cgccaacccg gcgaggagct gaatgaccgt
     1621 cacagggaca tcgcgcgagc gctgcaggtc gtcaccgaag aaatcgtgct gcatatggct
     1681 cgcgagctgc gtcgcagcac gaagatcaaa aatctgtgct tgagcggggg tgtcgcgctg
     1741 aactgtgtgg ctaatgcccg cgtactggcg gaggccgggt tcgagcgggt ttgggtgccg
     1801 ccatgcgcgt ccgacaccgg agcgcccctg ggaagtgcgc tttggcacta tcatcagaac
     1861 ctgggccatc cccgccggat ggagctaacg catcccttct acgggttgtg ctacagcgat
     1921 cacgagatag aaggcgcact agctgaggcc ggcttgacgg gtgagcggct aagcgaggcg
     1981 gagttgctcc gccgggtggc gcaggatctg gccgatggga agattgtcgg ctggtttcag
     2041 gggcgttcag aaatggggcc acgcgcgctc ggcaatcgct ccattcttgc cgatccacgc
     2101 cgccccgaga tgaaagacat tatcaatgcc cgcgtgaagc atcgtgagcc gttccggccg
     2161 ttcgcgcctg ctgtgcttct ggaccacgcg tcagagttct tccatctgga ccagccggat
     2221 cccttcatga ctctcgcgcc acgcgtgcga gcggaaaagg cacatctcat tcccgctgcc
     2281 gttcatgtcg atgggaccgg ccggattcag acggtgcaac gtagcgcgaa cccgctttac
     2341 tatggggtta tcgaagcgtt cggtaagctc acgggcgtgc ccgttctgct caatacgagc
     2401 ttcaaccgcc aggagccgat cgtggagact ccccggcacg ccgtgtcgtg ctacctgcga
     2461 accgggcttg atgtgcttgt gctgggcgcc tactacacga ccgaccgg
//
LOCUS       DASUMG010000051         3959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_408323, whole genome shotgun sequence.
ACCESSION   DASUMG010000051 DASUMG010000000
VERSION     DASUMG010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408323"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFX46_00835"
     CDS             complement(<1..548)
                     /locus_tag="VFX46_00835"
                     /inference="COORDINATES: protein motif:HMM:NF026192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HEX5599161.1"
                     /translation="MSKTQANAAAERKPLTGVEKATVLLLALSRSKAAQLLKRLDAEE
                     IRLIARAANRLPVLSAQDLAELVDEFTEMFSSGIGFAGSTSEVKDLLAQVMSEEEAEE
                     LLAEGAGKEEPVWARVARLKDEILRAYLLREHPQTMALILSRLDPTQAARIIGSLPLE
                     VRNALLVRMLGIRTVAPDALEV"
     gene            complement(562..954)
                     /gene="fliN"
                     /locus_tag="VFX46_00840"
     CDS             complement(562..954)
                     /gene="fliN"
                     /locus_tag="VFX46_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HEX5599162.1"
                     /translation="MLIQEFAAELTPTPEELAAAFGPSAKAEAPPSQQARQSRSPTAG
                     HLEAIMRIPVSVQVVLGSATMPVSGLVKLGRGAVIPLDRRVGEPVDVVVNGRVIARGE
                     VVVMDEGTSRFGISLTEVGAGSGSGRDA"
     gene            complement(1070..2020)
                     /locus_tag="VFX46_00845"
     CDS             complement(1070..2020)
                     /locus_tag="VFX46_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HEX5599163.1"
                     /translation="MIGAGAANSRTEVHRDALAAIYRCGAAYGQRLPMLPTALERVAA
                     ACTENLKGLSTAQPRVTYLGLESGKASELFGAERKEWLTGLLHALAWDAHVLVCVNYD
                     LIYTYVDIALGGDGSQQPFIEPRPLSRIETRIAQTFVARFAAALKIGFEPYAATLFAL
                     DGDMGSVNPDRLGGPHAPVAIARYRLEAGQCSGELQLAIPDAVLTALKPAFARPPLGR
                     TGTADPGWSQKIRGEINRTQLTMHAVLEDRPRTLAEVASFRVGQILPLEATARSPIRI
                     DCNGEPLLWGEVGQSNGVYTLRVQGFVDREQEFMNDILSG"
     gene            complement(2017..2907)
                     /gene="motA"
                     /locus_tag="VFX46_00850"
     CDS             complement(2017..2907)
                     /gene="motA"
                     /locus_tag="VFX46_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HEX5599164.1"
                     /translation="MTIIIGLIITLGALLGGFVAMGGHVNVIWQPWEYVIICGAAVGT
                     FVVANPVKTIKDVGVGFVEVLLMRTPKPREYLDVLGVLHELMRTLRSPSRNDLEHHIE
                     NPRESPLFQKFPTVLVNPELTTFICDYCRLILMGNARTHEIEALMDEEIETMRHDKLR
                     SYHALVSIAEALPALGIVAAVLGVIKAMGALDREPEILGSLIAAALVGTFAGIFFSYA
                     VVSPVASKLRTIREKKMQLYVVVKQTLLAFMNGAMPQVALEHGRKAISAYERPSIDEV
                     EEQTFSAGPGGNDSARKEAA"
     gene            3059..3841
                     /locus_tag="VFX46_00855"
     CDS             3059..3841
                     /locus_tag="VFX46_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HEX5599165.1"
                     /translation="MLTSIASYRLITNDLGRSLQMTARRPDVARETSYYLANIGKVKT
                     VEEFIGDHRLFAYAIRAFGLADLIYAKAFIKKVLTEGIDNPNSIANKLTDPRFREFVA
                     TFNFARYGELATSLERAQHGVVDKYVRQVLEEGAGQQNEGVRLALYFQRKAPELSNAY
                     QILADRALSEVVRTALGLPPLTAAIDVDRQAQMIEQRINFDDFKNPAKLESFITRFLS
                     LWELANPSTYSNPANLLIGRPIEAGFAPELLASFQNLRFARL"
     gene            3842..>3959
                     /locus_tag="VFX46_00860"
     CDS             3842..>3959
                     /locus_tag="VFX46_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HEX5599166.1"
                     /translation="MQSSLYVALSGQLALHRRLETIASNVANASSAGFRAQQV"
BASE COUNT          801 a         1242 c         1159 g          757 t
ORIGIN      
        1 aacacctcga gcgcatctgg agcgacagtc cggatcccca gcatcctgac caagagcgcg
       61 ttccgtactt ccaaaggcag cgagccgata attcgagcgg cctgtgtcgg atcgagacgc
      121 gacaagataa gtgccatcgt ctgcggatgc tcccgcagga gatacgctct aagaatttca
      181 tccttcagcc gcgcaaccct ggcccacacc ggctcttcct tgccagcgcc ttcagcgagc
      241 agctcttcag cctcctcctc gctcatcacc tgggcgagca ggtctttcac ctcgcttgtg
      301 gagccggcaa agccgatgcc gctggagaac atctccgtga actcatcgac gagctccgcc
      361 aggtcctgcg cggataacac tggcagccgg tttgccgccc gcgcgatgag ccggatctcc
      421 tcggcgtcta ggcgcttcag gagttgcgcg gctttcgagc gactgagcgc aagcaggaga
      481 accgtcgcct tttcgacgcc ggtcagcggt ttccgctcgg ctgccgcatt cgcctgagtt
      541 tttgacatcg gaccggatct gtcaggcgtc cctgccagat ccggagccag cacccacctc
      601 ggtcagggag atcccgaaac gggacgtgcc ctcgtccatg acgaccactt ctcccctcgc
      661 gatgacgcgg ccgttcacga ccacatcgac cggctcaccc actcggcgat ccaacggaat
      721 caccgcacct cgcccaagct tgacgagccc agaaacgggc atcgttgccg agccgaggac
      781 gacctggacg gagacgggga tacgcatgat ggcttcgaga tggccggctg tgggcgaccg
      841 cgattgccgg gcttgctgcg atggcggtgc ctctgccttc gccgacggcc cgaaggcggc
      901 agcaagctcc tctggcgttg gtgtcagctc agcagcgaac tcttgaatca gcaggtcttg
      961 tttctcagca gctgcgccag atggcgtgga gctctcagtg gcgcgcgaag gctgatcttg
     1021 cctgggcttc tgatggtgta ccgtgttccc cgcagcggac ggggcgcgat catccagaaa
     1081 ggatgtcatt catgaactcc tgctcgcgat cgacaaaccc ttggacgcgc aacgtgtaaa
     1141 ccccattgga ctgacccact tcgccccaga gcaacggctc accgttgcaa tcgatgcgaa
     1201 tgggtgaccg cgccgtcgcc tccagcggca aaatttggcc gacgcgaaac gaggcaacct
     1261 ccgccaatgt ccgcgggcga tcttccagca cggcatgcat tgtgagctgg gtgcgattga
     1321 tctcgcctcg gattttctga gaccatcccg ggtctgcagt gccggttcga ccgagaggcg
     1381 ggcgtgcgaa agccggtttc aaagcggtca aaacggcatc gggaatggcc aattggagct
     1441 cgccgctgca ttgccctgct tccaaccgat atcgcgcaat cgcaactggt gcgtgcgggc
     1501 cgccgagccg atctggattg acgctaccca tgtcgccatc caacgcgaaa agagtggcgg
     1561 cgtagggctc aaatccgatt ttcagcgctg cggcgaaccg ggcaacgaac gtctgggcga
     1621 tacgagtttc gatgcgcgac aacgggcgag gctcgatgaa tggctgctgc gagccgtcgc
     1681 cgcctagagc gatatcgacg taggtgtaaa tgagatcgta gttcacgcag acgagcacgt
     1741 gggcatccca cgcgagggca tgcagcaggc cggtgagcca ctccttgcgc tccgcgccga
     1801 aaagctcgga ggcttttccg ctttcgagcc cgagataggt caccctcggc tgcgcagtgg
     1861 ataggccttt aagattttcg gtgcatgcgg cggccacgcg ttcgagcgca gtcggcaaca
     1921 ttggcaagcg ctggccatag gctgccccgc agcgataaat ggcagcgaga gcgtcgcggt
     1981 gaacctcggt ccgtgaattg gcagcacccg caccgatcat gccgcctcct tccgagcact
     2041 gtcatttcca cccggccctg ccgagaatgt ctgctcctcg acctcgtcga tcgagggacg
     2101 ctcgtaggcc gaaatggctt tgcgaccgtg ctcgagtgcg acctgcggca tggccccgtt
     2161 catgaatgcc agcagcgtct gcttcaccac cacgtaaagc tgcattttct tttcgcggat
     2221 ggtccgcagc ttgctcgcga ccggtgagac gacagcatag gaaaagaaaa ttcctgcaaa
     2281 ggtgcccacg agcgccgcag caatcagcga tccgagaatt tcgggctccc ggtcgagcgc
     2341 acccatcgct ttgatgacac cgagcaccgc cgccacaata ccgagcgccg gcagggcctc
     2401 cgcaatcgaa acgagggcat ggtacgacct cagtttatcg tggcgcatgg tttcaatttc
     2461 ctcgtccatg agcgcctcga tttcgtgagt gcgcgcattg cccatcaaga tcagccggca
     2521 atagtcgcaa atgaaggtcg tcagctcagg attcacgagt accgtcggga acttctgaaa
     2581 gagcggcgat tccctcggat tttcgatgtg gtgctcaagg tcgttgcgcg acgggctgcg
     2641 cagcgtgcgc atgagctcgt gcagcacgcc gaggacatcc agatattccc gcggcttcgg
     2701 cgtacgcatg agaaggactt cgacgaaccc gacgccgaca tccttgatcg tcttcaccgg
     2761 gttggcgacg acgaacgtgc ccaccgccgc gccgcagatg atgacgtact cccatggctg
     2821 ccaaatgaca ttgacatggc cgcccatggc cacgaagccg ccaagcaagg caccgagcgt
     2881 gatgatgagc ccgatgatga tcgtcatgcg gagcctcccg tccgactctg atttgtctag
     2941 gtgccgaccc ttgcgcgggc cttaagccgg ttaacaaatg cgaggggatg aacgtcagct
     3001 ccgctcaagc cttgggatgc aaagctatac aaggcatttg atggggcggg cgcaacgcgt
     3061 gcttacgagc attgcgagtt atcgtctaat cacgaacgac ctcggccgct ctctccaaat
     3121 gacggcccgg aggccagacg ttgcgcgcga aacgtcctac tacttggcca acattggtaa
     3181 ggtcaaaact gtcgaggagt tcatcggcga tcatcgcctc ttcgcctacg cgatcagggc
     3241 tttcggcttg gccgacctga tctatgcaaa ggcgtttatt aagaaggtgc taaccgaagg
     3301 aatcgacaac ccgaacagca ttgcgaacaa gctcaccgat ccgcgctttc gggaattcgt
     3361 cgcaaccttc aacttcgccc gatatggaga gctagccacg tctctcgaac gtgctcagca
     3421 cggcgtcgtg gacaagtacg tgcgccaagt gctggaggag ggcgcgggcc agcagaacga
     3481 aggcgtgcgc cttgcccttt atttccaacg gaaggcgcct gagctttcga acgcgtacca
     3541 aattctcgcc gaccgggcgc tttccgaggt cgtgcggacg gcattggggc taccgcccct
     3601 gacagcagcc atcgatgtcg accggcaggc gcaaatgatc gagcagcgga tcaatttcga
     3661 cgatttcaag aaccctgcca agcttgagag tttcatcacg cgctttctca gcctatggga
     3721 gctggccaat cccagcactt attcgaaccc cgcaaacctt ctcatcggga gaccaattga
     3781 ggccggattt gcgccagaac tcctcgccag tttccagaat ctcagatttg cgaggctctg
     3841 aatgcagtcg agtctctacg tcgcgctctc tgggcagctt gcgcttcacc ggcgactcga
     3901 aacgatcgcc agcaacgtcg ccaatgcctc cagtgcgggc ttccgcgccc agcaggtga
//
LOCUS       DASUMG010000052         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_890380, whole genome shotgun sequence.
ACCESSION   DASUMG010000052 DASUMG010000000
VERSION     DASUMG010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890380"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="VFX46_00865"
     CDS             complement(<1..743)
                     /locus_tag="VFX46_00865"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HEX5599167.1"
                     /translation="MAGSELLLTLLGGVALLLWGVRMVRTGLTRAFGTPLRTALTRAS
                     KSRLAAFAAGVGVAGIMQSSTATALLLASFAGRNLVTLPVALAVMLGADVGTTLVAQI
                     FAFDIKWLWSAAAITGVVLFSVSEDDRTKNVGRIFIGLGLMLLSLTELGTVSAAMRAS
                     PTITAMMTALGNEPVIAVLVAAIITWIAHSSLAIVLFVLSLAAGGAIGPVLALTLVLG
                     ANLGGAVAPFTALTGSPVTARRVPLGNLLA"
     gene            complement(1161..1901)
                     /locus_tag="VFX46_00870"
     CDS             complement(1161..1901)
                     /locus_tag="VFX46_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599168.1"
                     /translation="MLTSVLEGSVSVGPYPELAGKRVLVTGLELQAGVDVARAFAEQG
                     CRIVLHTTASGAEADAVLEMLAQTASELQVYSDPLTDIESATRFAQTAAKTFSGVDAV
                     VNLIHLDLKALTAEALDGDFEAAIARRLQTARVITRIAANRMRLTWCEGSIFNVLVST
                     PPQTKAETILLSMARAMLASMTRSEASEWADQAIRINAVAPVLDSAEAGACLVSEPQV
                     AALAMFLTSSQGRDLSGLVFDLGLVEHA"
BASE COUNT          428 a          680 c          674 g          331 t
ORIGIN      
        1 gccagcagat tcccgagtgg aacccggcgc gctgtgacag gggaaccggt caacgccgtg
       61 aagggtgcca ccgcaccacc aaggttggcg ccgagcacga gcgtgagcgc caacacgggg
      121 ccaatggcgc cgccggctgc cagcgacagc acaaacagca cgatggcgag gctcgaatga
      181 gcgatccacg tgatgatggc ggccacgagc accgcaatca ccggctcgtt gccgagcgcc
      241 gtcatcatgg ccgtgatcgt cgggctcgca cgcatggcgg ccgagaccgt gccgagctcg
      301 gtcagcgaga gcagcatcag cccgagcccg atgaaaatgc gccccacgtt cttggtgcgg
      361 tcgtcctcag agacgctgaa cagcaccaca ccggttatcg cggcggcgct ccacagccac
      421 ttgatatcaa acgcgaatat ctgcgccacg agcgtcgtgc ccacatcggc gccgagcatg
      481 accgccagcg caacgggcag cgtgaccaga ttgcgaccgg cgaaggaagc gagaagaagg
      541 gccgtggccg tggagctttg catgatgcca gcgacgccca cgcctgctgc gaatgccgcg
      601 agcctgcttt tcgacgcccg ggtgagtgca gtgcggagcg gcgtgccgaa ggcacgcgtc
      661 aggcccgtgc ggaccatccg cacgccccag aggaggagcg cgacgcctcc cagcaatgtc
      721 aggagcagct cgcttccggc cattcctttc caacgctccg ctggaaacgc taatgcgaaa
      781 gaaagctaat gcgaaagatt gaaccgcttc ggctggccca atcacacgcg cgaagcaccc
      841 agcatactct ggtccggtgt caaaacctag gatcggccga gtgttcgcgc ggagaaaggc
      901 tcagcttgag ctgccgacct taccaagtct tcaagatggc gcagacttct ccggccagcg
      961 cacagcgcca ttctcatgcc ctgccaaacc gcagaacgcg gcgaggttcc cgctgcgact
     1021 tcgcgatgga gcgcgcacga cagcgtttga cctcatatgg gattgcagcc gcttccgtgc
     1081 caccgctatt tctcgcgatg agccggacgc cgggccaggg cccgggggcg gcacttaccc
     1141 gtgagcctgg aaacgcgcca tcaggcgtgc tcgaccaggc ccaggtcgaa gacaagccct
     1201 gacagatcgc gtccttgaga gctcgtgagg aacatggcca gcgcggccac ctgcggctca
     1261 gagacgagac aagcgccggc ttcggccgaa tcgaggaccg gagcaaccgc gttgatacgg
     1321 atggcctgat cggcccattc actggcttcg ctgcgggtca tgctcgcgag catggcgcgg
     1381 gccatggaca ggaggattgt ttccgccttg gtttggggcg gcgtgctgac gagcacgttg
     1441 aaaatcgagc cttcgcacca cgtcagccgc atgcgattgg cggcaatgcg cgtgatcacg
     1501 cgtgcggtct ggaggcggcg ggcgatggcg gcctcgaagt ccccatccag ggcctcggcc
     1561 gtgagggcct tgagatcgag gtggatcaga ttgacgacgg catcgacacc agagaacgtc
     1621 ttggcggccg tctgcgcgaa ccgcgtggca gattcaatgt cagtgagagg atcggagtaa
     1681 acctgcagct cggaagccgt ttgggccaac atctccagca cggcatcggc ttctgccccg
     1741 ctggcggtcg tatgcaatac gatccggcat ccttgttctg cgaaggcgcg ggccacatcc
     1801 acgcctgctt gaagctccag ccccgttacg agaacacgct tccccgccag ctcaggatag
     1861 ggaccaacgc ttacgctacc ctccagcaca ctcgtcaaca tgagcgctta cctcctcgca
     1921 ccgtcgggcc tcccctgccc gcacggcgcc actcaacgcc gattcgacta aacgactcct
     1981 aaatggcgcc aggattcggg tgcggccggg actgaagggc ggcgcaatta gggccgtaca
     2041 cagctgagtt ggatggtagc ttcgtaatcg ggcgaagtga acggataagg tcggctcaaa
     2101 acgatggcgg cgt
//
LOCUS       DASUMG010000053         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_87094, whole genome shotgun sequence.
ACCESSION   DASUMG010000053 DASUMG010000000
VERSION     DASUMG010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87094"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VFX46_00875"
     CDS             complement(<1..871)
                     /locus_tag="VFX46_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599169.1"
                     /translation="MNWSIDFAPMLPWPLIVAGAVVSLLLIGVLAFRRSRGTVLRALA
                     LGALILALMNPTLRQEERENLGNIAVVVMDESTSNRLADRSEQAQAIRADLEAKLGQI
                     PGLEVRWVTAARPDGQGPSGTNLFEELNRVLANTPPDRLAGVIMVTDGQVHDVPANAA
                     ALGFDAPVHTLLTGRPDEFDRRIEVLVAPKYAIVGQSRNVEIAVRETGRSQDSSERVT
                     LRVRREGRADELRRAQINERVRIPMHFPHAGQNILEVELTPAPGELTPANNRVVIAAE
                     GVRENLRVLLVSGE"
     gene            complement(888..>3227)
                     /locus_tag="VFX46_00880"
     CDS             complement(888..>3227)
                     /locus_tag="VFX46_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX5599170.1"
                     /translation="QAEASIVWLTDGIDHDESTAGFLERLSALGGSGLTVIEAGAGEE
                     PLGVAANVGSGGRLEVSVVRTGGGAREGVLHALSARGQRLGEAEFRFAPGQTVAKGHF
                     ELPLELRNQVTRVEIAGEPSAGAVHLLDARSQWNRIGLLSGESSEQAQPLLGPLYYIE
                     RALTPFAQLVQPRDPNLITGIDTILKQNASIIMLADIGTLTGEVKTRLEEWVQNGGIL
                     VRFAGPRLEKGGDDLLPVGLRAGGRTLGGALSWSTPQPLAPFPDDSLFAGLAVPQDVT
                     VSRQVLADPTQLGPDVKVWARLADGTPLVTASALGDGQLVLFHVTANSDWSNLPLSGL
                     FVEMLRRISTLGRLGGTGESTAAETSGTNASVSEASVLAPVQLLDGFGVLKPPPPTAQ
                     AISVADLPNLTPSAEHPPGYYGAQGAPRAFNLIAPDTVLRPLPGLPASAQRHAYEGQS
                     MTPLKPWLLATALGLLFADILAVVLLQMGGLALRRPVRRAARAAGALAIGVMLVQGMP
                     NQAEAQSATGVRDPVTMSAKDWLAVKATSKVTFGYVVTGNPEIDEISRSGLMGLGKVL
                     TLRTAVEPGEPIGVNIIDDEIAFFPILYWPVPADASALPEETLAKIDAYMKQGGMIIF
                     DTRDFGQGVPLGFSLQGGAGGTALQRLLGRLDIPRLEPVPDDHVLTKAFYLLRTFPGR
                     WDGGQLWVEAGMNDSLDGSRTARRADGVSSILVTSNDFAAAWAMDERGRPLYPTVPGG
                     ESQREMAYRTGINIVMYALTGNYKADQVHVPALLERLGQ"
BASE COUNT          581 a         1053 c         1018 g          575 t
ORIGIN      
        1 gctcgccgga aacgagcagc acgcgtagat tttcacgcac gccttcggcg gcaatcacca
       61 cgcgattgtt ggccggagtg agttcgcccg gggcgggtgt cagttcgacc tccaaaatgt
      121 tttgaccggc gtgggggaaa tgcatgggga tgcggacccg ctcattgatc tgcgcacggc
      181 gcagctcgtc agcgcgaccc tctcgccgca cccgcagcgt gacccgctcg ctagagtctt
      241 gcgaccggcc cgtttcccgc acagcaatct cgacgttgcg gctctggcct acgatcgcgt
      301 acttgggtgc gacgagcacc tcgatacggc gatcgaactc gtcgggacgc ccggtcagga
      361 gagtatggac cggagcatcg aagccgagcg cagctgcgtt cgccggcacg tcgtggactt
      421 gcccgtcagt caccatgatg acgccagcaa gccggtctgg cggcgtgttg gcgagcaccc
      481 tgttcagctc ttcgaacaaa ttggtgccgg atgggccctg gccgtccggt ctggccgcgg
      541 tcacccaccg aacctccaag ccaggaattt gccccagttt cgcctcgaga tcggcgcgga
      601 tcgcttgagc ctgctctgaa cgatctgcga gcctgttgct cgtgctttcg tccatgacga
      661 ccacggcgat gttgccgagg ttctcgcgct cctcctggcg gagagtcggg ttcatgaggg
      721 ccagaatgag tgcaccgagc gcgagcgcgc gcagcaccgt cccgcgcgag cgccgaaaag
      781 cgaggacgcc gatgagcaac agcgagacga ctgctccggc cacgataagt ggccacggca
      841 acataggtgc aaagtcgatg gaccagttca tcagttacgc ttgaacctca ctgtccgagc
      901 cgctcgagca gggcgggcac gtgcacctgg tcggccttat agtttccggt caaggcgtac
      961 atgacgatat tgatgccggt ccgataggcc atctcgcgct gactctcgcc gccgggcacc
     1021 gtggggtaaa gcggccggcc gcgttcatcc attgcccagg cagctgcgaa gtcattggac
     1081 gtcacgagaa tggaagagac cccgtctgca cgccgggctg tccggcttcc atccaggcta
     1141 tcgttcatgc ccgcttccac ccaaagctgg ccgccgtccc atcggccggg gaaagtcctg
     1201 agcagataga aggcttttgt cagaacgtgg tcgtccggca cgggctcgag ccttgggatg
     1261 tccaagcgtc cgagcagtcg ctgtagcgcc gtgccgccag ctccgccctg caggctgaag
     1321 cccagcggca cgccttgccc gaagtcgcgt gtgtcgaaaa tgatcatccc gccttgtttc
     1381 atgtaggcgt cgatcttggc cagcgtttcc tcgggcagcg cgctggcatc ggccgggacc
     1441 ggccagtaaa ggatcgggaa gaacgcgatc tcgtcatcga tgatgttgac gccgatgggc
     1501 tcgccgggct cgacggccgt tcgcagcgtc agcaccttgc ctaggcccat caagccgctg
     1561 cggctgattt catcgatctc cggatttccg gtcaccacat aaccgaaggt caccttgctc
     1621 gtggccttca ccgccaacca gtctttggcg ctcatcgtta ctggatcacg cacaccagtg
     1681 gccgattgcg cctcggcttg attgggcatg ccctggacca acatcacgcc gatcgcgaga
     1741 gctcccgcag cgcgtgcagc tcggcgcact ggacgcctca gtgcaaggcc gcccatctgc
     1801 aacaatacaa cagccagaat gtccgcgaac agcagaccca aggcagtcgc cagcagccaa
     1861 ggcttcagcg gtgtcattga ctgaccttcg tacgcatgtc tttgggcaga ggccggcaat
     1921 ccgggcagcg gcctcagcac cgtatcgggc gcgatcaggt tgaaggcacg cggggcgccc
     1981 tgtgcaccat agtatcccgg cggatgttca gcactgggcg tcaaattggg taagtcggca
     2041 accgaaatgg cctgggccgt tggcggcggt ggcttcagga cgccaaagcc gtcgaggagc
     2101 tgcacgggcg ccagcacgct ggcctctgaa actgatgcgt tggtgccgct ggtctcggcg
     2161 gcagttgatt caccggtgcc accgagccgg ccgagcgtgc tgatgcggcg aagcatttca
     2221 acgaacagcc ccgaaagcgg aagattggac cagtccgagt tggccgtaac gtggaacagc
     2281 acaagctggc catcacccag tgcactcgcg gtgacaagag gtgtgccgtc tgccagtctc
     2341 gcccatacct tcacgtcggg tccgagctgt gtcggatcgg cgaggacctg ccgactcacg
     2401 gtgacgtctt gaggtaccgc cagccccgcg aagaggctgt cgtctgggaa cggcgccagt
     2461 ggctgaggcg tggaccacga caaggcgccg ccgagcgtac ggccacccgc tcgcaatccg
     2521 acgggaagca gatcgtcccc gcccttctcg agccgagggc cggcgaagcg caccaatatg
     2581 ccgccgttct ggacccactc ctccagccgc gtcttcacct cgcccgtcag cgtaccgatg
     2641 tccgccagca tgatgatcga ggcgttctgc ttcaggatcg tgtcgatgcc tgtgatgagg
     2701 ttcgggtcgc gcggctggac caactgggcg aacggggtaa gcgcgcgctc gatataatag
     2761 agcggcccga gcaggggttg cgcttgctcg ctgctctcgc ccgagaggag gccgatccga
     2821 ttccattgcg agcgcgcatc gaggagatgg acggctcctg cggagggctc accggcgatt
     2881 tcgacccggg ttacctggtt gcgcagctcg agcggcagct cgaaatgacc cttcgcgacg
     2941 gtctgtcctg gcgcgaacct gaattcggcc tcgcccaggc gctgaccacg cgcagagagc
     3001 gcatgcaaca caccttcgcg ggcgccgcca ccggtccgca ccactgagac ttcaagccgg
     3061 ccgccgctgc cgacgttggc cgcaacaccc agaggttcct cgcctgcgcc cgcttcgatc
     3121 accgtcaggc cggaccctcc gagagcagag agtctttcca gaaagccagc ggtgctttcg
     3181 tcgtgatcta tgccatccgt cagccagacg atgctggctt cagcctg
//
LOCUS       DASUMG010000054         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_408673, whole genome shotgun sequence.
ACCESSION   DASUMG010000054 DASUMG010000000
VERSION     DASUMG010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408673"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="VFX46_00885"
     CDS             <1..1746
                     /locus_tag="VFX46_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HEX5599171.1"
                     /translation="VGVRLLRSRAVGLVTGSLLRRIFVSNLLGLFILIGGILYLSQHN
                     VWLIDAKRESLRTQSEIIAAAIAANATVETERIVLDPDRLPEAEDALIPFRDDGFAAL
                     QLSIRPERVAPILRRLVQPTNNRARIYSRDGTLIADSAQLLSRGLVAKPPQPKERPKT
                     KNFWTRLTKWLLNADLPVYKEIGNANGNFYPEVRMAMTGTTTPMLLLNENGEQIVSVA
                     APIQRARTVQGVLLLSSRPGEIDEALSQERWTVLKIGLMALVATLAASTLLARTVAGP
                     MRRLSEAAEHVSRNISARHDLPSFGNRSDEVGQMATAFRNMTTALFRRIEASEKFAAD
                     VAHELKNPLTAARSTAEALAYARDEAERQQLIGQIQGELKRLNRLITDVSNASRLDAE
                     LALQQMEPVALCKLLDGIVSTFSDILRDSGKSIAFEPDPSCSEDALVVNGHDGRLSQV
                     VTNLVDNAISFSPEGGMVLVRARRFGDWVEICVEDDGPGIAPENLEKIFRRFYTYRPT
                     PEASRGNNSGLGLSISREIVLAHGGEIWAENRMPEGAGPDDPPLGARFVVRLPAWAGG
                     GSQSRSFGHRGSRRI"
     gene            1727..2200
                     /locus_tag="VFX46_00890"
     CDS             1727..2200
                     /locus_tag="VFX46_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5599172.1"
                     /translation="MAVGESRLVHGTAIALKGRAALIRGESGSGKSDLALRCLAQPAS
                     DLISGQALLVADDQVRAEVRGGKVHLSCPETIKGLIEVRGLGIVAVPAQDEAELELLV
                     DLVRPDEVERLPDPAAVAELMGVQVRRLALAPFEASAPLKLLLALQGSAGAGRGS"
     gene            2358..2762
                     /locus_tag="VFX46_00895"
     CDS             2358..2762
                     /locus_tag="VFX46_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5599173.1"
                     /translation="MIGLLIVTHGELAREFRAALEHVVGPQEQIETISIGPDDDVEAR
                     RQEILDAARRLDTGDGVILLTDMFGGTPSNLSISILDEANVEVIAGVNLPMLVRLASV
                     RSNTPLAKAVDLAKEAGRKYISVARQVLSGES"
     gene            2768..3067
                     /locus_tag="VFX46_00900"
     CDS             2768..3067
                     /locus_tag="VFX46_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX5599174.1"
                     /translation="MSECESSTPSAFVTIRNRKGLHARASAKFVKCAESFNAKITVTR
                     DGNTVGGTSIMGLMMLAAGPGATLHLKAEGPEAPEALKALVELVENGFGEDCDGA"
     gene            3245..>3553
                     /locus_tag="VFX46_00905"
     CDS             3245..>3553
                     /locus_tag="VFX46_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX5599175.1"
                     /translation="MPTAADYAIKDIGLAEWGRKEIDIAESEMPGLMAVRAEYGPSQP
                     LKGARIAGSLHMTIQTAVLIKTLIALGAEVRWASCNIFSTQDHAAAAIAAEGVPVFAI
                     K"
BASE COUNT          681 a         1101 c         1118 g          653 t
ORIGIN      
        1 gtcggtgtgc gactgctgcg gagccgagcc gtagggttgg ttacggggag cctgctgcgg
       61 cgcatcttcg tttccaacct tcttggcttg tttattctca ttggcggcat cctttatctc
      121 agccagcaca acgtctggct catcgacgcc aagcgggaaa gtctcagaac ccagagcgaa
      181 atcatcgctg cggcaatcgc cgccaacgcg acggtcgaga cggagcggat cgtgctcgac
      241 ccggacaggc tgccggaagc cgaggatgcg cttatcccgt tccgcgatga cggtttcgca
      301 gcactgcagc tttcgattcg gcccgagcgt gtcgccccga tcctgcgccg gcttgtccaa
      361 ccgacgaaca accgcgctcg tatctacagc cgcgatggca ccctgatcgc cgactccgcg
      421 cagctcctgt cacgcgggct cgtcgccaaa cctccgcagc ccaaagagcg gcctaagacg
      481 aagaacttct ggacgcgact caccaagtgg ctgctcaacg cggatctccc tgtctacaag
      541 gagatcggca acgcgaacgg caacttctat ccggaagtcc ggatggcgat gaccggcacg
      601 accacgccga tgctgctgct caacgagaac ggcgagcaga tcgtctcggt cgctgccccc
      661 atccagcgag cacgtacggt gcagggggtt ctgctgctat ccagcaggcc cggcgagatc
      721 gacgaggcgc tgagtcagga gcgctggacc gtcctgaaga tcggtctcat ggcgcttgtt
      781 gccacgttgg ccgcatcgac gctgctggca cgtacggtcg ctgggccgat gcggcgcttg
      841 tccgaggcgg ccgagcacgt cagccgcaat atctctgcac ggcacgacct acccagcttc
      901 ggcaaccgca gcgacgaagt cggccagatg gcgacggcct tccgcaacat gacgactgca
      961 ttgttccgcc ggattgaggc gagcgaaaag ttcgccgcgg acgtcgcaca cgagctcaag
     1021 aacccgctga cggcggctcg ctcgaccgct gaagcacttg cgtatgcccg ggacgaagcg
     1081 gagcgtcagc agcttatcgg tcagatccaa ggcgaattga aaaggctcaa tcgcctaatc
     1141 accgatgtgt cgaacgcgtc cagactcgac gcggagctgg cgctccagca gatggagccg
     1201 gttgcgctgt gcaagttgct ggacggtatc gtctcaacgt tttcggacat tcttcgcgac
     1261 agtggcaaga gcatcgcctt cgagccggat ccgtcgtgct cggaggacgc gttggttgtg
     1321 aacgggcacg atggccgcct gtcgcaggtc gtaaccaacc tcgtggacaa cgcgatttcg
     1381 ttctcgcccg agggcggcat ggtgctggtg cgcgcccgca gattcggaga ttgggtcgaa
     1441 atctgcgtgg aggatgacgg tcccggcatc gcgcccgaaa acctggagaa gattttcagg
     1501 cgcttttaca cctaccgccc gacgcctgaa gccagccgtg gcaacaattc tggtctcggc
     1561 ctgtcgatct cgcgcgagat cgtgctggct catggcggtg agatctgggc ggagaaccgc
     1621 atgccagagg gagccgggcc ggatgatccg ccgttgggcg cccgcttcgt cgtgcgtctt
     1681 ccggcctggg ctggcggcgg atcgcagtcg aggagctttg gacatcgtgg cagtcggcga
     1741 atctaggctg gtccacggaa ctgcaattgc gctcaaaggc cgcgctgcgc tgattcgcgg
     1801 tgagtccggc tccggaaaat cggacctcgc cctgcgctgc cttgcgcagc ccgcttccga
     1861 tctcatctca ggacaggcgc tgctagttgc cgacgatcag gttcgcgccg aggtccgcgg
     1921 cggtaaggtc cacctcagct gtcctgagac gatcaagggg ctgatcgagg ttcgcggact
     1981 tggcatcgtt gccgtaccgg cccaagacga ggcagagctc gagttgctgg tcgatctcgt
     2041 tcgtccggac gaggtcgagc gcctgcctga tccagctgcc gtggccgagc tcatgggcgt
     2101 acaagttcga cgcctcgccc tggccccttt cgaggcatcc gcaccactaa agctattgct
     2161 ggcgctgcag gggtctgcgg gtgccggtcg tggttcatag tcgtccctca gtaggcatac
     2221 ggtgtgaaag cgcttgctat gcttggatca gttgccaaac atgtcgcggc tcgggaccgc
     2281 cagttacctg ctagggacat gcacggaccg gttggccgac ggatggccac cagccagcac
     2341 ggaactgcac gagagcaatg ataggacttt tgatcgtcac ccatggcgaa ttggcccgcg
     2401 agttccgggc cgcactggag catgtcgtcg ggccgcagga acagatcgaa acaatctcca
     2461 tcggacctga tgatgacgtc gaggcgcgcc ggcaagaaat cctggacgcc gcgcgcaggc
     2521 tggataccgg cgatggcgtg atcctgctca cggacatgtt tggcggcacg ccatccaacc
     2581 tctccatctc gatcctcgat gaggccaacg tagaagtcat tgctggagtg aacctgccga
     2641 tgctggtccg cctcgccagc gtccgcagca atactcctct cgccaaggcc gtcgatctcg
     2701 ccaaggaagc tggccgcaag tacattagcg tcgccaggca ggtgctttcc ggcgagtcgt
     2761 aaaagagatg tccgagtgcg agagttccac gccctccgct ttcgtgacca ttcgcaaccg
     2821 aaaggggttg catgcgcgcg cgtcggcgaa gttcgtcaag tgcgccgaat cgtttaacgc
     2881 caaaatcacc gtcactcggg acggcaatac ggtgggcggc acctccatca tggggctgat
     2941 gatgcttgcg gcgggccccg gcgcgacgct ccacctcaaa gccgaaggac cggaagcccc
     3001 ggaagctctc aaggcgcttg tcgaactggt cgagaatggc ttcggcgaag attgcgacgg
     3061 agcttaagaa gcgtgctccg ctcttgttat aacacttgca gacataacga tgtctttatg
     3121 tgccattgca taaggacacc ctggccgcta tagacggcca ggcacgagcc agacgcgtca
     3181 gcgataacct tcgctgtgca ccgccgccag aggcagaatt ccgaaaatcg aggagagccg
     3241 agtgatgcct actgcagccg actacgccat caaggacatc ggccttgccg aatgggggcg
     3301 caaggagatc gacatcgcgg agagcgaaat gccgggcctg atggcggttc gcgcagagta
     3361 cggcccttcc cagccactca agggcgcccg catcgctggc tcgctgcaca tgacgatcca
     3421 aacggcggtg ctgatcaaga cgttgatcgc gcttggcgcc gaggtccgct gggcttcctg
     3481 caacatcttc tccacgcagg atcatgccgc agcggcgatc gcagcagagg gcgtgccggt
     3541 gttcgccatc aag
//
LOCUS       DASUMG010000055         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_7181, whole genome shotgun sequence.
ACCESSION   DASUMG010000055 DASUMG010000000
VERSION     DASUMG010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7181"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VFX46_00910"
     CDS             complement(<1..731)
                     /locus_tag="VFX46_00910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX5599176.1"
                     /translation="MQAGNLVVELEELVSRIPDGASLAIPAEYSGCAAAAVRALIRRG
                     CRDLHLIPVPTGGFMADWLIGAGCVSSVEAAAMTLGEHGTPPRFSAAIREGRIRMKDS
                     TCPAIHAGLQAAEKGIPFIPLRGILGSDLLRYRSDWRVINNPFSEHDDPIVLLPAIRP
                     DVALIHARAADRHGNVWVGVRREMMLMAHAAQATLVTVERIEDTDFLTDDAMAAGTIP
                     ALYISALSVVPGGARPIGLAGSYPA"
     gene            981..2126
                     /locus_tag="VFX46_00915"
     CDS             981..2126
                     /locus_tag="VFX46_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5599177.1"
                     /translation="MSRVQLHKFIDLAKPIRTREERHLELSRRGFLSAAAGLAALTSM
                     SKDAFARNFGPDAPPIRYPDPDVVVLDKRFKAKLGNTPIMRLYTGTLWAEGPAWSGVG
                     RYLVWSDIPNDENLRWLEEDGHVGRRFRYPAGNSNGNTFDFEGRQIACQHGTRKVVRY
                     EHNGKVTVLADKYDGKELNAPNDAVVHPDDGSIWFTDPGYGSLMEYEGNRLPESASSV
                     SPIQKEAIYRIDAQTGEITKVADEPFKPNGLCFSPDYKKLYVADTGITHYPEAKSQIW
                     VYDVDGTKLKNPQTFVSMEWDGKTGFADGIRCDEDGNVWSSAGWVGDGYDGVHIFAPD
                     GTRIGQIRLPEICSNVCFGGSKRNRLFMTASQSLYAVYVETRGAHIT"
BASE COUNT          515 a          717 c          672 g          389 t
ORIGIN      
        1 tcagccggat aagaacccgc cagaccgatc gggcgcgcac caccaggaac aaccgacagc
       61 gccgagatat acagggccgg gattgtacca gccgccatcg catcgtcggt caagaaatcc
      121 gtgtcctcga tacgctcgac cgtcacaagt gtcgcctggg cggcgtgcgc catcagcatc
      181 atttcgcggc ggacaccgac ccaaacgttg ccgtggcggt cagccgcgcg agcatggatc
      241 agtgccacat ccgggcggat ggcaggcaga agcacaatag gatcgtcgtg ctcggagaac
      301 ggattattga tcactctcca atccgaacga tatcgcagga gatccgaacc cagaatgccc
      361 cggagcggta tgaatgggat tcccttctca gccgcctgca accccgcatg gatcgcaggg
      421 caggtcgaat ccttcattcg gattcgaccc tcccgaatag cggctgaaaa gcgcggcggg
      481 gttccatgct cgccaagcgt catagcggcc gcttcaacac tggacacaca tcctgcgccg
      541 atcaaccaat cggccatgaa gccaccggtc gggactggga tcagatgcaa atcgcgacag
      601 ccacggcgaa tgagcgctct taccgccgcg gcagcgcaac ccgagtactc cgccgggatt
      661 gccaagctgg cgccgtctgg aatgcgcgac acaagctctt ccagctccac gacgagatta
      721 ccggcttgca tagatcgagc tccttagttg tccaaaggcc tactttgatg agagccaagc
      781 gcctgcacaa gccgcggttg atgtgctgta atgcagccta cctcgcatcg gcggcatgtc
      841 ttgccggggc gcggcacgta gcatgtgctg acggagctca gccggcccgc ggggacactc
      901 gggaatcgag ctgaagccgg gagggctcgt cagcagcctg atcgtaaaaa acaatccaca
      961 gtttcggagg aaaccgggcc atgtctcgtg tccagcttca caagttcatc gatctcgcca
     1021 aaccgatccg cacgcgcgaa gagcgccatc tcgaattaag ccgtcgcggt ttcctcagcg
     1081 cagcggctgg cctggcagcg ctgacctcca tgtcgaagga cgcgttcgct cgaaactttg
     1141 gcccggatgc gccgccgatt cgatacccag atccggacgt cgtggttctc gacaagcgct
     1201 tcaaggcgaa gctcggcaac acgcccatca tgcggctcta cactggcacg ctgtgggccg
     1261 aagggccagc gtggagcggc gttgggcgtt atctcgtctg gagcgatatt cccaacgacg
     1321 agaacctgcg ctggctcgag gaggacggcc atgtcggccg ccgattccgc tatcccgcgg
     1381 gcaacagcaa cggcaataca ttcgactttg aagggcggca gattgcctgc cagcacggta
     1441 cacgcaaggt ggtccgttat gagcacaacg gcaaggtaac tgtgctagcc gacaagtacg
     1501 acggcaaaga gctcaacgcg ccgaacgatg ccgtcgtaca ccccgacgat ggttccatct
     1561 ggttcaccga tcccggctat ggctcactca tggagtacga gggcaaccgg ctgcccgaaa
     1621 gtgccagcag cgtgagtccg atccaaaaag aagccatcta ccgcatcgac gcccagacgg
     1681 gcgaaatcac gaaagtggcg gacgagccgt tcaagccgaa cgggctgtgc ttctcacccg
     1741 attacaagaa gctttatgtc gccgacaccg gcatcaccca ctatccagag gcgaagagcc
     1801 agatctgggt ctacgatgtc gatggcacaa agctgaagaa cccacagacg ttcgtcagca
     1861 tggaatggga tggaaagact ggtttcgccg acggcatccg gtgcgatgag gacggcaatg
     1921 tctggtcaag cgccggctgg gtcggcgatg gttacgatgg cgtgcacatc tttgcgcccg
     1981 acggcacacg catcggccaa atccggctgc cggagatctg ctccaacgtc tgcttcgggg
     2041 gcagcaaacg caatcgactg ttcatgacgg ccagccagtc gctctacgct gtctacgtcg
     2101 aaacccgcgg agcccatatc acctgacgaa agcggcccct cccgaataca aacgggaggg
     2161 gctttcgatc caggtgtttc ccttgcacac ggcgccgcag caggttagca atccgcgcca
     2221 gagcccattc aacgacacgg aacgcgtacg aaatgcccac gtacaatctg ctgctgctgc
     2281 ctggcgatgg aat
//
LOCUS       DASUMG010000056         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_7230, whole genome shotgun sequence.
ACCESSION   DASUMG010000056 DASUMG010000000
VERSION     DASUMG010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7230"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFX46_00920"
     CDS             <1..604
                     /locus_tag="VFX46_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEX5599178.1"
                     /translation="FLIKQVVEELAREKPTLKTFVTLSPVPSFARWLATELEQDETGL
                     IGPDIREALSSLADHTWLDHEEQVRALEGPLTMLAAHYLVNAKGKDGRPLDPVARFHL
                     GNGARLERINWLADTSPKGLRESHGIMVNYRYDLKEFERNHEAYANEGVVATSRAVRN
                     LLRSSVKVRPRTEPRQLPPPDKTQEEEVREPDEIIEPAGR"
     gene            complement(656..1771)
                     /locus_tag="VFX46_00925"
     CDS             complement(656..1771)
                     /locus_tag="VFX46_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599179.1"
                     /translation="MTVSDSNDDHGNIPTPKPPRGSGLYTATWAVLAVTATGYMAALL
                     LEPEWAAPLTTQSLRAEEPSSAQPELVEQLTGEVNRLRETISDLQRELTEVKAKAAAR
                     EVEIINQRKRAEIDPPEVPEEREPVTAELQQEESELLDEPAQVETAAVEETDVGLRAT
                     DTEPTYSDVPPDRPQVVQQSTDMPTLAAAEEPQERRPHIEILNVRREGAGDESGTEQI
                     ELAETTPAVMPPPPTPVKKIAALRARPEIASAAAKGQEALPMATASLPRREPPAIVFG
                     PAIVTPASEAFAIRLETGPSLDALRLRWSVLSERHQSALRDLEPRYLAVGTPSHPSYE
                     LLAGPITSLEEASRICALLRDNMIPCSVGGQFEGEAL"
     gene            2098..>3231
                     /gene="glmU"
                     /locus_tag="VFX46_00930"
     CDS             2098..>3231
                     /gene="glmU"
                     /locus_tag="VFX46_00930"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5599180.1"
                     /translation="MTSRPLLTIVLAAGKGTRMKSAVPKVLHRVGGLSLIGHVLTAIR
                     SLGESRTAVVIGPDMDGVRAEVAKIAPEAEVFVQEQQLGTAHAVLAARTAIARHDGDV
                     LVVFGDTPFITPATLEGLLASLDATTPVAVLGFHAADPNGYGRLIFDGPEQVSAIREQ
                     EDATEAEREITFCNSGVMAFRGEQMLTLLEAIGSRNAKGEYYLTDAVAIARSKGYRVA
                     AHSCVEEEVMGINSRNQLAQAEAVFQRRFRERVMAEGATLIAPETVWFSHDTVIGRDV
                     IIEPNVFFGPKVVVEDEVVIRANCHIEGARLRRGAEVGPFARLRPNADIGPRAKIGNF
                     VEVKNVTVEEGAKANHLSYLGDGRVGAGANIGAGTIFCNYDGFF"
BASE COUNT          593 a          992 c         1021 g          625 t
ORIGIN      
        1 ctttttgatc aagcaggtcg tcgaggagct ggcgcgcgag aagccgacgc tgaagacgtt
       61 cgtgactttg tcaccggttc cgtcgtttgc gcgctggcta gccactgagt tggagcagga
      121 tgaaaccgga ctgatcgggc cggacatccg ggaagctctg agctcattgg cagatcacac
      181 gtggctggat cacgaggagc aggtgcgggc actcgagggg ccattgacga tgctcgccgc
      241 gcattacctt gtgaatgcga aggggaagga cggccgtccc ttggatccag tcgcccgctt
      301 ccacctcggg aacggggcgc ggctcgagcg catcaactgg ctagcggata cctccccgaa
      361 aggtctgcgg gaatcacacg gcataatggt gaactaccgc tatgacctga aggagttcga
      421 acgcaatcac gaggcctatg ccaacgaggg cgtggtggcg acgtcccggg ccgtgcgcaa
      481 cctgttgcgg tcatccgtga aggtccgtcc gcgcacagag ccccgccagc ttcctcctcc
      541 cgacaagacg caggaagaag aggtccgcga acccgacgag atcatcgagc cggcagggcg
      601 ttgaggcgag cttcacggta agccgcctcc gagggcgcca gaagccgcac gctgttcaga
      661 gtgcctcgcc ctcaaactgt ccgccgacgc tgcacggaat catgttgtcc cggagcagcg
      721 cgcaaattcg gctcgcttcc tccagcgatg tgatcggacc ggcgagaagc tcgtaggatg
      781 gatgactcgg cgtgccgact gcgagatagc gcggctccag atctctgagc gcgctctggt
      841 ggcgctcgct gagcacgctc caccgcaacc gaagtgcatc gagtgacgga ccggtctcaa
      901 ggcggatagc aaacgcctct gaggcaggtg tgactatagc cgggccgaat acgatcgcgg
      961 gtggctcgcg ccgtggcaag ctggcggtcg ccatcggcag agcctcctgg cctttagcag
     1021 cagcagacgc aatctcgggc ctcgcacgca acgcagcaat cttcttaact ggcgtcggag
     1081 gcggcggcat gaccgccggt gttgtctccg ccaactcaat ctgttcggtt ccggactcgt
     1141 ccccggcgcc ttctctcctg acatttagga tttcaatgtg aggccgacgt tcctgaggtt
     1201 cctccgcagc cgccaacgtc ggcatgtcag tcgactgttg tactacttgc gggcggtcgg
     1261 gcgggacgtc cgaataagtc ggctcagtat cagttgctct gaggccaaca tccgtctcct
     1321 cgacggcagc agtctcgacc tgcgcaggtt cgtccaacaa ctcagattct tcctgctgga
     1381 gctcagctgt caccggctct cgctcctcgg gcacctctgg tggatcaatc tcagcgcgct
     1441 ttcgctggtt gatgatttcc acttccctgg cagctgcctt cgctttgacc tcggtcagct
     1501 ctcgctgcag gtcggaaatg gtctcgcgca gtctgttcac ctcgcccgtc agttgctcga
     1561 ccagctcagg ttgggcggag gaaggctcct ctgcccgcaa cgactgtgtc gtaaggggag
     1621 cggcccattc cggctccagc aacaacgccg ccatgtagcc cgtcgcggtt accgccagca
     1681 cggcccatgt tgcagtataa agcccagagc cacggggtgg cttaggagtg gggatattgc
     1741 cgtgatcgtc gttggagtcc gaaacggtca tttgcgctcg catggccgcc cttgatctct
     1801 ctttcgcttg ggcgcgggat acacgcgcga ctcagtccgc gcggccaagc taccgattcg
     1861 cttgcgccaa accgtcctct gagtccgtta tcccggtcaa cggatcgggg tggaaagggt
     1921 gggcctggcc tgtggaaact ctcgaacttg ccgcatcctg gccatatgcc ttgtctccgg
     1981 ctcgtccgag tggggaagcg agccagctga gattacggag cgttttgtct agaagaggcg
     2041 cggaagtgag cttaactcgg ggctccggcg gacacacaaa cggctggtgg tgaattcatg
     2101 acctctcgcc cactgcttac gatcgtcctg gcggccggca aaggcacgcg catgaagtcg
     2161 gccgttccga aagttcttca ccgcgtcggc ggcctctcgc tgattgggca cgtcttgacc
     2221 gccatccgga gccttggcga aagccgcaca gccgtcgtca tcggtccgga catggatggc
     2281 gtccgtgccg aagtggcgaa aatcgccccg gaggcggaag tcttcgtcca ggagcagcag
     2341 ctcggcacgg cacatgcggt gctcgcggcg cgaacggcta tcgcacgtca tgatggagac
     2401 gttctggtcg tcttcgggga tacgccattc atcacgccgg cgacgctcga gggcctactc
     2461 gcgtcactgg atgcaacaac gcctgtcgcc gtgctcggct tccatgccgc cgatcccaac
     2521 ggatacggcc gcctgatctt cgacggtccg gaacaggtca gtgccatccg cgagcaggag
     2581 gatgcgaccg aggctgagag ggaaatcacc ttctgcaact cgggagtaat ggcgttccgg
     2641 ggcgagcaga tgctcacgct tctggaggcg atcggctccc gaaatgccaa gggtgaatac
     2701 tatctcaccg atgccgtcgc gatcgctcgc tcgaagggct atcgcgtggc cgcccactcc
     2761 tgtgtggaag aggaggtgat gggcatcaac tcgcgcaatc agctcgctca agccgaggcc
     2821 gttttccaac ggcgattccg cgagcgggtg atggccgagg gtgccactct gatcgcaccg
     2881 gagacggtct ggttcagtca tgacacggta atcggccgcg acgtaattat cgagcccaac
     2941 gtgttcttcg gaccgaaggt cgtggtcgag gacgaggtcg tcatccgcgc caactgccat
     3001 atcgaagggg cacgcttgcg ccggggtgcc gaagtcggac cgtttgcccg gctccggccg
     3061 aacgcagaca ttggcccgcg tgcgaagatc ggcaatttcg tcgaggtcaa gaacgtcacc
     3121 gtcgaggagg gcgcaaaggc caatcacctt tcctatctgg gcgacggccg tgtcggtgct
     3181 ggtgcaaaca tcggtgcagg aactatcttc tgtaactacg acggcttctt c
//
LOCUS       DASUMG010000057         2538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_328555, whole genome shotgun sequence.
ACCESSION   DASUMG010000057 DASUMG010000000
VERSION     DASUMG010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328555"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1101)
                     /locus_tag="VFX46_00935"
     CDS             complement(1..1101)
                     /locus_tag="VFX46_00935"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX5599181.1"
                     /translation="MALRELIAENAPVALSAGGLLIGFLFGAIVFKTNFCTMGSISDI
                     VSFGDYRRFRAWVLAAVTALVGAQLLQLSGVVELTRSMYLSPNVNWFGHVLGGLMFGY
                     GMVFAGGCPSRNLARVGGGDLRSLVALIVIGIFAFMTIGGILGPIRAWLEQSTAVSLA
                     GLGAPSQSIGDVLSVSLGLDASTAALIATIGVAVAALAYCFGDENFRTSPVHIASGIG
                     IGLCVIAGWALTGLAFDEMADRPVAPISLTFVRPAGDTLEWLQRYTALGLPGFGVATV
                     FGAILGSFVTAVAMKRFTLTTFSDKGDTIRVLSGAALMGVGGVMALGCTVGQGITGVS
                     TLAIGSFISFAAIVTGGVIGMKAFERMLMAEA"
     gene            1299..1739
                     /locus_tag="VFX46_00940"
     CDS             1299..1739
                     /locus_tag="VFX46_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HEX5599182.1"
                     /translation="MTVHMIKLCVGADSIEDLAQWQKGRLAAQKAAGQKPQLFHRTRM
                     VPKRQAELLDGGSLYWVIKGVIQVRQRIIGFGEGQKPDGTRCCLILLDRKLVPTHPVP
                     RRAFQGWRYLEPADAPPDLDARTSQQLAQLPPEMRKELAELGLI"
     gene            1809..>2538
                     /locus_tag="VFX46_00945"
     CDS             1809..>2538
                     /locus_tag="VFX46_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5599183.1"
                     /translation="MAELPATVPFVGPEAQERSRGRPFRARIGANESVFGPSPRAIEA
                     MRQAAADCWMYADPENHDLKAALAKHHSVGFENIVVGEGIDALLGYTVRMLIEPGDPV
                     VTSLGAYPTFNFHVAGFGGKLVRVPYRGDREDPEALLDAARREKAKLVYFANPDNPMG
                     SWWGASEVQRLIDDLPPGTVLLLDEAYSDTAPADAIPPIDVSNPQVLRLRTFSKAYGL
                     AGLRVGYAIGEAGLIRSFDKIRNHF"
BASE COUNT          521 a          797 c          792 g          428 t
ORIGIN      
        1 tcacgcctcc gccatcagca tccgctcgaa agccttcatg ccgatcacgc cgccggtgac
       61 gatggcggcg aacgagatga acgagccgat ggccagcgtc gacacgcctg taattccctg
      121 accgaccgtg cagccaagcg ccatcacccc gccgacgccc atcagcgccg cgccggagag
      181 cacacgtatc gtgtcgcctt tgtccgaaaa ggtggtgagc gtaaatcgct tcatggcgac
      241 ggcagtcaca aaggagccca gaattgcgcc gaacacggtg gcgacgccga agcctggaag
      301 cccgagagcg gtatagcgct gcagccactc gagcgtgtcg cccgctggcc tgacgaatgt
      361 cagagagata ggagccaccg gccggtcagc catttcatcg aatgcaagcc cggtcagagc
      421 ccagcccgcg atcacgcaga ggccgatacc gatgccagaa gcaatgtgaa ccggtgacgt
      481 gcgaaagttc tcgtcaccga agcagtaagc cagggcagca acagcgactc cgatcgtagc
      541 aatcaacgca gccgtcgatg cgtccaagcc caggcttacg ctgaggacat cgccgatgct
      601 ctgactcggc gcgccgaggc cggcgaggct gacggccgtc gattgctcga gccaggcccg
      661 gatggggccc aagatcccgc cgatggtcat gaaagcgaaa attccgatga cgatcagagc
      721 cacgagcgac ctcagatctc caccgcccac gcgggcgaga tttcggctcg ggcaaccgcc
      781 tgcgaacacc atgccatatc cgaacatcag cccgccaagc acgtggccga accagttgac
      841 gttgggtgag aggtacatcg acctcgtcag ctcgacgacg ccggaaagct gcagaagctg
      901 ggcgccgaca agggcggtca cggcggcgag cacccaggcg cggaagcggc gataatcacc
      961 gaatgacacg atgtccgaaa tggagcccat cgtgcagaaa ttggtcttga agacgatggc
     1021 cccgaacaag aagccgatca acaggccgcc agcgctgagg gccacgggcg cattttcagc
     1081 gatgagttcg cgaagtgcca tgatcagctt cctccctggc gcaacggatg ggcgtacgac
     1141 agtatattcg cagatttgaa tatgatcggg caagcgtggg ctgaaggtcc ccgttagccg
     1201 tcttctgcac acccagtagc tgctcccggc tcgcttcggg tggcggtagg gtgtatcagc
     1261 tggcatgccg tgatgcagca cgaggaacgc gacatccgat gaccgtgcac atgatcaagc
     1321 tgtgcgtcgg tgcggacagc atcgaggatc tggcccaatg gcagaagggc cggctcgccg
     1381 cgcagaaggc tgctgggcag aagccgcagc tgttccacag gacgcgcatg gtcccgaagc
     1441 gccaggcgga gctgctcgac ggcggctcgc tttactgggt gatcaagggt gtgatccagg
     1501 ttcgccagcg cattatcggc ttcggagaag gacagaagcc ggacggcacg cgctgttgcc
     1561 tcattttact cgatcgcaag ttggtgccga cgcacccggt tccgcgacgc gccttccagg
     1621 gctggcgcta cctggagccg gccgacgcgc caccggatct tgacgcccgc acctcgcagc
     1681 agctcgccca gcttcccccc gaaatgcgca aggaattggc tgagctcggc ctcatctgac
     1741 ctcgaacttg tttttcacgg agagccacag tgccacagta tccgcgcgga ccccgctaca
     1801 cccgcataat ggcagagctg ccggcgacag tgccgttcgt cggtccggag gcacaggaac
     1861 ggtctcgcgg ccgaccgttc cgcgctcgga tcggggcgaa cgaaagtgtt ttcggcccgt
     1921 cgccgcgcgc gatagaggcc atgcggcagg cagctgcgga ctgttggatg tacgccgatc
     1981 ccgagaacca cgatctgaag gcggccctgg cgaaacatca tagtgtcggg ttcgagaata
     2041 tcgtggtggg cgaggggatc gacgcgttgc tcggctatac cgtgcgaatg ctgatcgagc
     2101 cgggagatcc tgtcgtcacg tccctcggcg cttatcccac cttcaacttt cacgttgcag
     2161 gctttggcgg aaagctcgtc cgcgtgccat accgcggtga tcgcgaagac ccggaggcgc
     2221 tcctcgatgc agcgcggcgg gaaaaagcca agctcgtcta tttcgcgaat ccggataatc
     2281 cgatgggttc gtggtggggc gcgtctgaag ttcagcggct aattgatgat ctgccgcccg
     2341 gcaccgtcct gctgctcgac gaggcttact ccgatactgc acctgcagac gccattccac
     2401 cgatcgacgt ctccaatccg caggtgctaa ggctacgcac gttctcgaaa gcctatggat
     2461 tggccggcct gcgtgtcggc tatgctatcg gtgaggctgg cctgatccgc tcgttcgaca
     2521 agatccgcaa tcacttcg
//
LOCUS       DASUMG010000058         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_810623, whole genome shotgun sequence.
ACCESSION   DASUMG010000058 DASUMG010000000
VERSION     DASUMG010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810623"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="VFX46_00950"
     CDS             <1..446
                     /locus_tag="VFX46_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5599184.1"
                     /translation="AYGIYAAYIAAHLLMVLIVAVAAVIMLRISQVKLSILAPVVLVL
                     CVIGAYAVNNTMNGVYVLLIFGVVGYALVKTGFPLAPLILGLILGQQIELNLLRAIMT
                     DDNLWLFITRPISGGLLLVALISVVLSIWQHVRHARRTATVEPDF"
     gene            complement(479..2101)
                     /locus_tag="VFX46_00955"
     CDS             complement(479..2101)
                     /locus_tag="VFX46_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX5599185.1"
                     /translation="MNPYDQHLDRNPANYQPLTPLTLLDRAAKVHPDRVAVIHGQQRM
                     TYRQFYARCRQLASALDRHGIGRGDTVSVMLSNTPPMLEAHYGVPMTGAVLHSINTRL
                     DAGVIAFQLDHSESKVLIADREFAPTIRAALDLVKSRPLVIEYDDKEFPQPGDPLPGT
                     DYEAFVSGGDPEFAWRMPNDEWDAIALNYTSGTTGNPKGVVYHHRGAYLMCFANVVAA
                     GMGRHPVYLWTLPMFHCNGWCFPWTLSAVAGTHVCLRWVRAKAMYDAIAEHRVTHLCG
                     APIVMSTLLNATPEEKKPLPHKVQFVTAAAPPPESVLAAMAEAGFDVTHVYGLTETYG
                     PAVVNDWHDEWDALPPSERAAQKARQGVRYPTLEGLTVMDPKTMTEVPADGQTLGEVM
                     FRGNIVMKGYLKNPKATEEAFAGGWFHSGDLGVLHPDGYIQLKDRSKDIIISGGENIS
                     SIEVEDVIYKHPAVASAAVVAKPDEKWGETPCAFVELKPGATASEDEIIEWCRQHLAR
                     FKVPRQVVFQEIPKTSTGKIQKFLLREKAKSL"
     gene            complement(2188..>2696)
                     /locus_tag="VFX46_00960"
     CDS             complement(2188..>2696)
                     /locus_tag="VFX46_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5599186.1"
                     /translation="CYGWEHRDDPIAVYGGGEKGAKLALMMKLWSDDIVLCTDGRERP
                     SNETFRQLTRNGIAVRADKIGQLDGTGTQLERIVFANGDVLPRRALFFTVGQHQGSSL
                     FQRLGVRQMQTGGLDAAWPSCRTDVDGVFVAGDASRDVQLLVVAVAEGACAALAINKA
                     LLAEDGLG"
BASE COUNT          484 a          861 c          811 g          540 t
ORIGIN      
        1 tcgcctatgg catctacgca gcgtacatcg cggcgcacct gctgatggtg ctcatcgtgg
       61 ccgtcgcggc cgtgatcatg ttgcggatct cgcaagtcaa gctctcgatc cttgcccctg
      121 tcgtgctcgt cctgtgcgtc atcggcgcct acgccgtgaa caacacgatg aacggcgtct
      181 atgtgctgct cattttcggc gtcgttggct atgcgctcgt caaaacggga ttcccgcttg
      241 ctccgctcat cctcggcctc atcctgggcc agcagatcga gctcaatctc ctccgggcga
      301 ttatgacgga tgataacctg tggctgttta tcacccgccc gatctcggga ggtctgcttc
      361 tcgtcgcgct aatatctgtt gttttatcga tttggcagca cgtacggcac gcacggcgca
      421 ccgcgactgt tgagccggat ttctgatcgc tgctttaacc tctgacgacc tgaggccgtc
      481 agaggctctt cgccttctcg cgaagcaaaa acttctggat cttgcccgtt gaagtcttcg
      541 ggatctcctg gaacacgact tgccgcggta ctttgaagcg cgccaggtgc tgacggcacc
      601 actcgatgat ctcatcctcg cttgcggtgg caccaggctt gagctccacg aaggcgcagg
      661 gtgtttcacc ccatttctca tccggcttgg caacgacagc ggcagatgcg accgccggat
      721 gcttgtagat cacgtcctcg acctcgatcg acgatatgtt ctcgcccccc gagatgatga
      781 tgtccttcga gcggtccttg agctgaatgt agccgtccgg atgcagcacc cccaggtcgc
      841 ccgaatggaa ccagccgccc gcgaacgcct cctccgtcgc cttcgggttc ttgaggtagc
      901 ccttcatgac gatgttgccg cggaacatga cctcgccaag ggtctggccg tccgcaggca
      961 cctcggtcat cgttttcgga tccatgacgg tcaggccctc gagcgtcggg tagcgcacac
     1021 cctggcgggc cttttgcgct gcccgttccg aggggggcaa cgcatcccat tcatcgtgcc
     1081 agtcattgac caccgctggg ccataggtct cggtcaaccc atacacgtgg gtgacatcga
     1141 acccggcctc cgccatggcc gcgagaacag attcgggcgg cggcgccgcg gcggtgacga
     1201 actgcacctt gtggggcagc ggcttcttct cctccggcgt tgcattgagg agcgtcgaca
     1261 tgacgatcgg cgcgccgcag agatgcgtaa ccctgtgctc tgcgatggcg tcgtacatgg
     1321 ccttggcccg aacccagcgc aggcacacat gagtgccggc caccgccgac agggtccacg
     1381 ggaagcacca gccgttgcag tgaaacatcg gcagggtcca gagatagacc ggatgccggc
     1441 ccatgcccgc cgccacgacg ttggcgaagc acatcaggta cgcgccgcga tggtggtaaa
     1501 ccacaccctt cggattgccc gtggtgcccg aggtgtaatt gagagcgatc gcatcccact
     1561 catcgttcgg catccgccac gcgaattcgg gatccccgcc ggagacgaag gcctcgtagt
     1621 ccgtgcccgg tagcggatcg ccgggctgcg gaaactcctt gtcgtcgtat tcgatgacga
     1681 ggggccgcga cttcaccaag tccaaagctg cgcggattgt cggcgcgaac tcgcggtcgg
     1741 caatcaggac cttggactcg gagtgatcga gttggaacgc gataacccca gcatccaacc
     1801 gcgtgttgat ggaatgcagc acggcgccgg tcatgggcac gccgtagtgc gcctcgagca
     1861 tcggcggcgt gttggacagc atgaccgaaa ccgtatcgcc acgaccgatg ccgtgccgat
     1921 ctagcgccga ggcaagctgg cggcatctgg catagaactg gcggtaggtc atccgctgct
     1981 gcccgtggat gacagccacc cgatccgggt gcacctttgc agcgcggtca agcaacgtca
     2041 gcggggtcag cggctggtaa ttcgccgggt ttctgtcgag gtgctggtcg taaggattca
     2101 tgggcgtaag gcacattcag gagacagcac cggacgctac tccatgcggc acctcggcgg
     2161 caatgcgatt gaatgtgaat cgtgcgctca gccgagaccg tcctcggcga gaagggcttt
     2221 gttgatggcg agcgctgcgc aggctccttc ggccactgcg acgacgagca gctgaacgtc
     2281 tcgcgaagca tccccggcca cgaagacccc atccacatcc gtgcggcacg atggccaggc
     2341 cgcgtcgaga ccgccggttt gcatttggcg gacaccgaga cgttgaaaca gacttgaacc
     2401 ttgatgctgg ccgaccgtga agaacaaggc tcgacgcggc agcacgtctc cgttggcgaa
     2461 cacgatccgc tccagctggg tgcctgttcc atcgagctga ccgatcttgt ccgccctgac
     2521 ggcgatgcca ttcctggtga gctggcgaaa cgtctcgttg ctaggccgtt cgcggccatc
     2581 cgtgcacagc acgatgtcgt cgctccacaa tttcatcatg agagcgagtt tggcgccttt
     2641 ttcgccgccg ccatacaccg caatcggatc atcacggtgc tcccagccat agcagt
//
LOCUS       DASUMG010000059         2996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_810750, whole genome shotgun sequence.
ACCESSION   DASUMG010000059 DASUMG010000000
VERSION     DASUMG010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810750"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..2911
                     /locus_tag="VFX46_00965"
     CDS             329..2911
                     /locus_tag="VFX46_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5599187.1"
                     /translation="MAGKPAFPQNQLVSPAMREIEAQQGPLTADIPLVSSRSGGGVIA
                     IAAVVLAGALGTLAVLTFDSSEPILLTAMAFLATLGVFFVFGLVAGHIRIADHSGNAN
                     ITDALARSFKDGLCVTTLDGRRVYANPAFDELVGPTNYDHVSALESLCAGEPQAAEAL
                     FRLARAAGRGESRTEEFRLRTVVAGQRQSRWFRLSVSRLQLGRSGESEPLVLWRMTDV
                     TDDRRREAEAMRALEATLGYYETMPLGLLVAGDDAEIEHLNDTLAKWLGLSASSLARG
                     LKLTDIVSGDGALLLRALARDAEHTARRIDLDLVREDGVIWPATLLVVQRSQQAGFVA
                     AVLERTTEPNAGGRGRTADVRFARFFQSAPFGIATVGADGRIVSANAAFARLLLDGKA
                     GRGGRAVDVLTRNVEPDRRAEVEAALNDALAGKANIQPLEITVGESRELTRRVFFSAL
                     SRGSAEEAAVLYVIDATELKALEMQFAQSQKMEAIGKLAGGVAHDFNNVLTAIIGFSD
                     LLLQTHRPGDPAYRNIMNIKTAANRAGELVSQLLAFSRRQTLQPEVLQIGELLTDTSV
                     MLNRLLGEKIELKIQPGRDLWYVKADYSQLQSVIINLAVNARDAMPDGGQLIIRTRNV
                     AERDCQKLEPQNLPVGEYVMIEVEDTGHGMTPEVRAKIFEPFFTTKSVGKGTGLGLAT
                     VYGIVKQTGGYVFVDSTPGKGTTFRIFLPRHVVDAEDEVEVAAGRNGRKERHRDLTGS
                     GRVLLVEDEDAVRNFAAEALKRQGYEVLQASSGAEAIEMVRTADRPVDLVISDVIMPE
                     MDGPSMGRQLQEINPDIKIIFMSGYPDDALNNSLDPNVPFAFLQKPFTLAQLAAKVKE
                     ELAK"
BASE COUNT          553 a          940 c          975 g          528 t
ORIGIN      
        1 gatggaggcg tgcacgtcct tatggcgctg tgagggcgcc cccgccccgt ccggccatcc
       61 cgttcgcacc ccccgacggt cgcagcgcga aggtatagcg cgcttcgcga aacagattaa
      121 cacgattgga accagcacaa ctcgtgcgcg aacagaacat ctctgtacgc aaaaagaccg
      181 gtgaacggac actcggcaac ggtatgttta ccggaatgac tgaaatattg agatcgttac
      241 ggttccgaga cgtccttcct cccagtgagg gcggccgtag cctgggctta tgaacattcg
      301 tgaaagttgt gcgtaagagt agcctaagat ggccggcaag cccgcctttc cccagaatca
      361 gctagtctct cccgccatgc gggaaatcga agcgcagcag ggaccgctca cggctgacat
      421 tccactggtg agttcgcgtt ccggcggagg cgtcatcgcc atcgccgcgg tcgtgctcgc
      481 cggggctttg gggacacttg ccgtcctcac gttcgattcg agcgagccca ttctcctcac
      541 cgccatggcg tttctggcga cgcttggcgt cttcttcgtt tttgggctcg tggcagggca
      601 cattcggatc gctgaccact ccgggaatgc gaacatcacc gatgccttgg cgcggagctt
      661 caaggatggc ttgtgcgtca ccaccctcga cggccggcga gtttatgcca accctgcctt
      721 cgatgagctg gtcgggccga cgaactacga tcacgtcagt gccctggagt cgctctgtgc
      781 cggcgagccg caagctgccg aagccttgtt ccggctggcg cgggcggcag gccgcggaga
      841 aagcagaaca gaggagttcc ggctgcggac tgtcgtggcc ggccagcggc agtctcgctg
      901 gtttcgcctc agcgtaagcc ggcttcagtt gggccggagt ggagagtccg agcccctcgt
      961 cctgtggcgg atgaccgacg tcaccgatga tcgtcggcgc gaggccgagg cgatgcgcgc
     1021 attggaagcg acgcttggct actacgagac gatgcccctc ggcctcctgg tcgccggaga
     1081 cgatgcggag atcgagcacc tcaacgacac gttggcgaag tggctcggcc tttcggccag
     1141 ctcgctggca cgcggtctga agctgaccga tatcgtttcc ggagacggcg ctctcctctt
     1201 gcgcgcgtta gcccgcgatg ccgagcatac agcacgccgg atcgatctcg atctggtacg
     1261 cgaggacggc gtcatatggc cggcgacgct gcttgtcgtg cagcgttcgc agcaggcggg
     1321 ttttgtggca gccgtgctcg agcgcactac ggagcccaac gcgggtgggc gcggccgcac
     1381 ggccgatgtc cggtttgccc gcttcttcca gtcagcgccg ttcggcatcg cgacggtcgg
     1441 cgcggatggc cgaatcgtca gtgccaacgc ggccttcgcg cggctgctgc tggatggtaa
     1501 ggccgggcgt gggggccgcg ccgtcgacgt tctaacccgc aacgtcgagc ccgatcggcg
     1561 ggcggaggtg gaagccgcgc tcaacgatgc cctggccggg aaagccaata tccaacccct
     1621 cgaaataaca gtcggcgagt cgcgcgagct cacgcgccgc gtcttcttca gcgccctgag
     1681 ccgcgggtcg gcggaggagg cggcggtgct ctacgtcatc gatgccaccg agctgaaggc
     1741 gctggagatg cagttcgccc agagccagaa gatggaggcg atcggcaagc tcgcgggcgg
     1801 cgtcgcgcac gacttcaaca acgtgctgac ggccatcatc ggtttctcgg acctgctgct
     1861 ccagacccat cgccccggcg atcccgctta tcgcaacatc atgaacatca agacggcggc
     1921 aaaccgcgcg ggcgagctcg tgagccagct gctcgccttc tcgcgccgtc aaaccctgca
     1981 gccggaggtg ctccagatcg gcgagctgct gacggacacc tccgtgatgc tcaatcgcct
     2041 cctgggcgag aagatcgagc tgaaaatcca gcccgggcga gacctgtggt acgtcaaggc
     2101 cgactactcg cagctccagt ccgtcatcat caatctggcc gtaaatgcgc gggacgccat
     2161 gcccgacggc ggccagctca tcatccgaac aaggaacgtt gcggagcgcg attgccagaa
     2221 gctcgagcca cagaacctgc cggtcgggga atacgtcatg atcgaggtcg aggataccgg
     2281 ccacggcatg acgcccgagg tgcgcgccaa gatcttcgag ccgttcttca ccacgaagag
     2341 cgtcggcaag ggaaccggcc tggggctcgc gacggtctac ggcatcgtca agcagacggg
     2401 cgggtacgtc ttcgtcgaca gcacgccggg caagggtacg acgttccgca ttttcctgcc
     2461 gcgccatgtg gtcgatgcgg aggatgaggt cgaggtcgct gcaggccgga acgggcgtaa
     2521 ggagcgccat cgcgatctga ccggctccgg ccgcgttctg ctggtggagg atgaggacgc
     2581 ggtccgcaac ttcgccgcgg aggccttgaa gcggcagggc tacgaagtgc tgcaagcctc
     2641 aagcggggcc gaggcgatcg aaatggtgcg cactgccgat cgcccggtgg acctcgtcat
     2701 cagcgacgtg atcatgccgg aaatggacgg ccccagcatg ggccggcagc tgcaggagat
     2761 caatccagat attaagatca tcttcatgtc cggctatcct gatgacgccc tcaataacag
     2821 cctcgatccc aacgttccct tcgccttcct gcagaagcca ttcacattgg ctcagctcgc
     2881 ggcgaaggtg aaagaggagc tggcgaaata gccggtgttg ctagcctggc agcgggacac
     2941 gagagcgagt tgcccgtgac gatcccttgc gtcggttccc gcgatcataa gaaatc
//
LOCUS       DASUMG010000060         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_891228, whole genome shotgun sequence.
ACCESSION   DASUMG010000060 DASUMG010000000
VERSION     DASUMG010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891228"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..672)
                     /locus_tag="VFX46_00970"
     CDS             complement(145..672)
                     /locus_tag="VFX46_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5599188.1"
                     /translation="MLAEIYVKRGVDQSLARQVAQQLMAKDALTAHARDELGISEFTT
                     ARPVQAALTSAATFSVGAALPLLMVVVSPAGALVAIVSAASLGFLALLGAIGATAGGA
                     NILRATARVTFWGAFAMALPVSGNYSERLFELMALAGRLVNDVLCALVRQGDNYPPPV
                     PWSKAARARWCWLAT"
     gene            895..2199
                     /locus_tag="VFX46_00975"
     CDS             895..2199
                     /locus_tag="VFX46_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HEX5599189.1"
                     /translation="MDLRAADGVALRFHAYIEHLSEAMGHADRHAPLAEYCTGLLLPG
                     ERKSIEPMAARVAPAEVGAKHQSLHHFVAKAPWEESKLLASVCSYVLPILEKMAPMRA
                     WIVDDTGIPKKGRHSVGVARQYCGELGKQDNCQVAVTLSVANDHASLPIACRLYLPEA
                     WTEDEARRKKAGVPDEIAFATKPQIALDQIGQAIEDGVPKGIVLADAGYGNDSGFRSG
                     VTELGLTYVVGVQGSTTVWTPGTAPLPAKPWSGRGRRSKLLRRDGETRPVKVSDLAKA
                     LPRKAWKTVCWREGSHGDLVGRFAAVRVRPSHRDYWRAEPWPEEWLLIEWPEGEDAPT
                     KYWLSTLPADAAVATLVDTAKLRWRIERDFQDLKQEIGLDHYEGRGWRGFHHHAALSI
                     AAYGFLVAERSPIPPSASFIRALGRKIPTHQADYRPRGAAAA"
     gene            2482..2952
                     /locus_tag="VFX46_00980"
     CDS             2482..2952
                     /locus_tag="VFX46_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX5599190.1"
                     /translation="MKKFSLALAIPLLLLAPAASAQTGKADKPEAGQKAAAKMSAEEF
                     LTKTAKANQFEIDASKLAVDKAKSKEVKDLAQGLVSDHTAAAEKLKAAVQADGKLTLP
                     KDDKAYAKHAKTLEKLKDASGEAFDRPTSRPCSPGTRSRSSATRVTRRAATTPL"
BASE COUNT          595 a          941 c          978 g          477 t
ORIGIN      
        1 gagccgcagc cgctcggccc tttccgcgcc gatcgcttgg cggtgaagat catctcctcg
       61 atgaggcgca cgttgggagc agtccgctga tattgtggga gacgccaagg ccggcgtcac
      121 cgcgaacgtg gcgtggggcg atgatcatgt cgcaagccag caccacctcg cgcgcgccgc
      181 cttcgaccat ggcaccgggg gtgggtaatt atcgccctga cgcaccaggg cgcaaagcac
      241 gtcgttgacc agcctgccgg ccaatgccat gagttcaaac aaccgctccg aataatttcc
      301 cgacaccggc agagccatcg caaacgcgcc ccaaaaagtg acgcgagctg tggcgcgcaa
      361 aatgtttgca ccgcctgctg ttgccccgat tgcacccaaa agagcaagaa agcccaagga
      421 ggcagcagac acgatcgcga caagggcccc ggccggcgag acgacgacca tcagcaaggg
      481 cagggcggca cctacagaga atgttgcggc cgatgtcagt gcggcttgca caggacgcgc
      541 ggtggtgaac tccgaaatcc caagctcatc gcgcgcgtgg gcggtcaggg catccttcgc
      601 catcagctgc tgggcaacct gccgggcaag ggactgatcg acgccacgct tgacgtagat
      661 ttcggctagc aggctgctga aataggattt tcgaccctgg aaggtgacgt tctacgttga
      721 gcagagccca ctttgacgac gtcgggtcgc attctgctca acgagagacg caatttcgag
      781 agtctatgca accgagtgct ccgagttttt cagcagcctg ctagtcttac tgtgtcataa
      841 aattacgatc catgctatgc agcagattct tgattcgttt cgagcgaggc gaatatggat
      901 ctgcgcgccg ctgatggagt ggcgttgcgt tttcatgcgt acatcgagca tctgagcgag
      961 gcgatggggc acgccgatcg gcacgcgccg ctcgccgagt attgcacggg cctgctgctg
     1021 ccgggcgaga ggaagagcat cgagccgatg gcggcgcgcg tggcgcctgc cgaggtcggc
     1081 gccaagcatc agtcgctgca tcacttcgtg gcgaaggcac cttgggagga gtcgaagctt
     1141 ctcgcatcgg tgtgctcgta cgtgctgccc atcctggaga agatggcgcc gatgcgggcc
     1201 tggatcgtcg acgacaccgg catcccgaag aaggggcgcc actcggtggg cgtcgcgcgc
     1261 cagtactgcg gcgagctcgg caagcaggac aactgtcagg tggccgtgac gctgtcggtc
     1321 gccaacgacc atgcgagcct gcccattgcg tgccggctct atctgccaga ggcgtggacc
     1381 gaggacgagg cgcggcgcaa gaaagccggc gtgcccgacg agatcgcgtt cgccaccaag
     1441 ccgcagatcg cgctcgacca gatcgggcag gctatcgagg atggcgtgcc gaagggaatc
     1501 gtgcttgccg atgccggcta cggcaacgac tctggcttcc gcagtggggt caccgagctg
     1561 ggcctgacct acgttgtcgg tgtgcaaggc tcgacaacgg tctggacgcc cggaaccgcg
     1621 ccgttgccag cgaagccttg gagtggccga ggtcgaagaa gcaagctgct gcgacgcgac
     1681 ggcgagaccc ggccggtcaa ggtcagcgac ctcgccaagg cgttgccccg caaggcctgg
     1741 aagaccgtgt gctggcgtga gggcagccat ggcgatctcg tcgggcgctt cgccgccgtg
     1801 cgcgtacggc cgtctcaccg cgactactgg cgggcagagc catggccgga agaatggctg
     1861 ctgatcgaat ggccggaggg cgaggatgcc ccgaccaaat actggctctc gaccctgccc
     1921 gccgacgctg ccgtggcaac gctggtcgat accgccaagt tgcgctggcg gatcgagcgc
     1981 gacttccagg atctgaagca ggagatcggc ctcgaccatt acgagggacg aggttggcgc
     2041 ggcttccacc accacgcggc gctctcgatc gcggcctacg gattcctcgt cgccgagagg
     2101 agtccgattc ccccctcagc ctccttcatc cgtgcactcg ggcgcaaaat cccgacgcac
     2161 caggcagact accgcccgcg cggcgccgct gcggcctgaa cggcatacgt ccaactccat
     2221 ctcgacgatc cggcgattga tcgccaccgc gcttgccaac cgcttgccgc gctgcccctg
     2281 ttgtctgcag atgcgtcaga acaagcgcag gcgttcgtga cacagtaaga ctagctagtt
     2341 ccgagttggg ccgcttggct cctgttcctg gcctcggacg aagcttccgg cacatgcagc
     2401 gcggaaccgg aacggcgcgg tctggttcga ctcgcaagaa ggctacgagc ctgatccgtt
     2461 ccgcaaattg gagatgtcgc catgaaaaag ttcagtcttg cactggctat cccgctgctg
     2521 ctgctcgcgc ccgcggcgag cgcgcaaacg ggcaaagccg acaagccgga ggccggccag
     2581 aaggccgcgg cgaagatgag cgcggaagag ttcctcacca agaccgcgaa ggccaaccag
     2641 ttcgagatcg acgcgagcaa gctcgccgtc gacaaggcca agagcaagga ggtcaaggac
     2701 ctggcgcaag ggcttgtctc ggatcacacc gctgccgccg agaagctcaa ggcggcggtt
     2761 caggcggacg gcaagttgac gctgccgaag gacgacaagg cctacgccaa gcacgccaag
     2821 acgctcgaga aactcaagga cgcctcgggc gaggcgttcg accgacctac ctcgaggcca
     2881 tgctcaccgg gcacaaggag tcgctcaagc gctacaaggg ttacgcggcg agcggcgaca
     2941 acgccccttt gaagaagttc gccgaggaga tcgcgcccat cgtggcggcg c
//
LOCUS       DASUMG010000061         2240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_409651, whole genome shotgun sequence.
ACCESSION   DASUMG010000061 DASUMG010000000
VERSION     DASUMG010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409651"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFX46_00985"
     CDS             complement(<1..563)
                     /locus_tag="VFX46_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX5599191.1"
                     /translation="MKAVTILGATGSVGTSTLDIIRRAPDKFRVAAVTAQTNAAKLAQ
                     IAIEHRAELAVIGDEACYGELRDRLAGSGIEVGAGPSALIAAAEHPADCVVAAITGAA
                     GLAPTLAAARLGRRLALANKECLVAAGDIFMATVRNSGTELVPIDSEHSGVFQLLAGA
                     DTRAIERIVLTASGGPFRGWSLERLAQA"
     gene            complement(640..1491)
                     /locus_tag="VFX46_00990"
     CDS             complement(640..1491)
                     /locus_tag="VFX46_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX5599192.1"
                     /translation="MTSDENQVTRASALFSELKPRLLSGLLLAAVALGLCYLGPLSFA
                     VLVVIVALIMCWEWGRVVRGREFDTAFMIHGGAVAAASLLAALGLAALGLLLVMIGAV
                     LVLLLQYGARGRLSALGVPYVGVPAISLLWIRWDEPFGFAAVLFVLLVVCATDTFAYF
                     GGRGIGGARLWPRVSPNKTWAGLISGIVAGTVVGALFAFFVEGSSHVGLALLAFLLAL
                     ATQAGDLTESALKRGCGVKDASTLIPGHGGFLDRMDGVVFAAFAAAIVALLIDPYAPA
                     RALLLGS"
     gene            complement(1488..>2240)
                     /locus_tag="VFX46_00995"
     CDS             complement(1488..>2240)
                     /locus_tag="VFX46_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX5599193.1"
                     /translation="VDAGERSPPPRHVAIIMDGNGRWAKRRGLPRSVGHRMGVEALRR
                     TVRAAIELGISYLTIYTFSSENWSRPADEVNELMRMMKRFVRRDLVDLHENGVRIRVI
                     GERNNIDPDLLRLIEEAESLTANNERLTLVVAFNYGSRIEITKAARRLAEKAARGLLD
                     PAQITPELFGAELDTSGIPDPDLLIRTSGEVRLSNFLLWQAAYAELMFIDAYWPDFGR
                     EHLKQAIAEYCSRDRRYGGLPRESKGLKTHHP"
BASE COUNT          451 a          751 c          700 g          338 t
ORIGIN      
        1 gcctgtgcga ggcgctccaa gctccagccg cggaacggtc cgcccgaggc ggtgagcacg
       61 attcgctcga tcgcacgggt gtctgcgcca gcgaggagct ggaagacgcc ggagtgctcg
      121 gagtcgatgg gcaccagctc cgtgcccgag ttcctcacgg tcgccatgaa gatgtcgccg
      181 gccgcaacca ggcattcctt gtttgccaag gccagccggc gcccgagacg ggcggccgcc
      241 agtgtcggcg caagtccggc cgcgccggtg atggccgcga caacgcaatc ggcgggatgc
      301 tcagcggcgg caatgagggc cgagggccct gctccaacct cgatcccgct gcccgcgagc
      361 cgatcgcgca gctcgccgta gcaagcctcg tcaccgatga ccgccaactc cgcccggtgc
      421 tcaattgcaa tctgggcaag cttggcggca ttcgtctgcg cggtcacagc cgcaacccgg
      481 aatttgtcgg gagcgcgccg gataatatcg agcgtgctag tcccgaccga gcccgtcgct
      541 ccgagtatgg tgaccgcttt cacttagcct caaccattga agaaggtgtt tggactgccg
      601 ccaaattgct cgacccgaac attcctgctg cccaataggt catgatccca gcaggagtgc
      661 acgagccggc gcgtagggat cgatcaggag cgcgacgatc gccgcagcga aagccgcgaa
      721 cacaacgccg tccatccgat ccaagaagcc gccgtggccc gggatgagcg tgctcgcgtc
      781 cttgacgcca cacccgcgct tgagggccga ctcggtcaaa tcgccggcct gcgtcgccag
      841 cgccaataga aaagcaagga gcgccagccc aacgtgcgag ctgccctcga cgaagaacgc
      901 aaacagggct cccacgacgg ttccagcgac gatgccgctg atgaggccgg cccaggtctt
      961 gttcggtgaa acacgtggcc acagccgggc gcctcctatc ccgcgccctc cgaagtaggc
     1021 aaacgtatcc gtcgcgcaca cgacgagcaa gacaaagagc actgcggcaa acccgaacgg
     1081 ctcgtcccat cgaatccaaa gaagagagat ggctggcaca ccgacgtatg gaacgccgag
     1141 cgccgaaagt cgaccgcgcg ctccatactg cagaagcagg acgagcacgg caccgatcat
     1201 caccaggaga agcccgaggg ccgccaggcc aagcgcagcg agcagagaag ctgccgcaac
     1261 cgcacctccg tggatcatga aagcggtgtc gaactctcgg ccgcgaacga ccctgcccca
     1321 ctcccagcac atgatgagcg cgacgatgac gacgagcacg gcgaagctga gcggcccgag
     1381 gtaacagagc cccagcgcta ctgcggccag caacagcccg gagagaaggc gcggcttcag
     1441 ctcactgaag agcgccgatg cccgtgtcac ctggttctcg tcagaggtca tggatgatgc
     1501 gtcttcaggc ccttcgactc gcggggcagt ccaccatacc gacggtctcg ggagcaatac
     1561 tcggcgatag cctgcttgag gtgctcgcgg ccgaagtccg gccagtacgc atcgatgaac
     1621 atcagctccg catacgccgc ctgccagaga aggaagttcg acaggcggac ttcgccgctc
     1681 gttcgaatga gcaggtcggg gtccggaatg ccgctcgtgt ctagctcagc cccaaacagc
     1741 tcaggtgtga tctgggcagg gtccagcaga ccgcgcgctg ccttctcggc gaggcgccgg
     1801 gccgctttcg tgatctcgat ccgggaaccg tagttgaagg cgacgacaag cgtgagccgc
     1861 tcgttgttcg cggtcagcga ctctgcctcc tcgatcagcc tgagcagatc gggatcgatg
     1921 ttgttccgct ctccgatgac gcgaatgcga accccgttct cgtgcaaatc gacaaggtcc
     1981 ctgcgcacga accgcttcat catgcgcatc agctcgttga cctcgtcggc ggggcgcgac
     2041 cagttctccg aggagaaggt gtagatcgtc agataggaga tgcccagctc gatggcggcc
     2101 ctgaccgtcc ggcgcaatgc ctccactccc atccgatgac caacggaacg cggcaggcca
     2161 cggcgttttg cccaacggcc gttgccgtcc atgatgatcg cgacgtggcg cggcggcggg
     2221 ctacgctcgc ccgcatcaac
//
LOCUS       DASUMG010000062         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_972063, whole genome shotgun sequence.
ACCESSION   DASUMG010000062 DASUMG010000000
VERSION     DASUMG010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972063"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VFX46_01000"
     CDS             <1..1051
                     /locus_tag="VFX46_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5599194.1"
                     /translation="LEPEQPAVILFTSGTEGEPKGVALSSRNLVANAEQIYAFTEGVL
                     QPDDILFNPLPIFHSFGLTGGTLLPIFHGIKTVLYPSPLHYRAVARLIGETKATILIA
                     TDTFLQGYARAARPEDLGSIRYAVAGAERVKDTTRAMWSKTGAVLLEGYGVTECSPVL
                     AVNVPETNKPGTVGKMLPAIEYRLAPVEGLKNAGRLSVKGPNVMLGYMLPENPGVISP
                     PPDGWHDTGDIVSIDEEGFVTIRGRAKRFAKIGGEMVSLAAVESVISTLWPEGYHVVV
                     SAPDPHKGEQLVLVTDMADADRDTLIRHMHEEGLPALWVPKAIYVVPAIPVLGSGKVD
                     LVATIELVKQKLPFL"
     gene            1132..>2634
                     /locus_tag="VFX46_01005"
     CDS             1132..>2634
                     /locus_tag="VFX46_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599195.1"
                     /translation="MSPNVGALAAEKQGTLNTWRNIWRERLEAAVLTPIREFRLAYLP
                     LLMIYFAYGALGITAVADSFWVKLSLTLTPEDLAALGVWLTLPWTVKMVFGELVDAVP
                     LLGSQRRAYIFLGAGMVATGLVLLAGAAGGWITFASPDTIYVLASLLTVTGVVLQDVV
                     ADAMSTEVVARENPDGTPRDKAAVDRDLGMVQVLGRLALSLGIFSVAWLGGYLAQVLS
                     YETIFLIGLVVPLISITGAALVRLETAAPRPIDWRLLAGGLAFGVVVTLLGVTGLPFN
                     QEIIFVVSITVIILMLKRVVAGVDDETRKRIFYAALVIFAFRAVPGTGVGYQWFSIDR
                     LGFDEAFFGVLQQIGAGIGLVAAWLLSDSITRYPIARVMLWLTILGAILSIPNLILVH
                     ELHVWTEQVLGIGARQIAILDTAVESPLLNISMIPMLTLIAIYAPAGHRATWFALMAS
                     LMNLALVAGQLMTKYLNMLFVVNRGDYIALPALTWSAVILGLVIPLFVILR"
BASE COUNT          429 a          814 c          847 g          544 t
ORIGIN      
        1 gcttgagccc gagcagccgg cggtcatcct gttcacatcc ggcacggaag gcgagcccaa
       61 gggagtcgcg ctgtcgagcc ggaacctcgt cgccaacgct gagcagatct acgccttcac
      121 cgagggcgtg ctccagcccg acgatatcct cttcaatccg ctgccgatct tccattcgtt
      181 cgggctgacc ggcgggacgc tgctgccgat ttttcacggc atcaagacgg tgctctatcc
      241 gagccctctt cactatcggg cggtcgcgcg gctgatcggc gagaccaagg caacgatcct
      301 cattgcgaca gatacgttcc tccagggcta cgcacgagcg gcccggcctg aggatctggg
      361 cagcatccgg tatgcggtcg cgggcgccga gcgcgtcaag gacacgacca gggccatgtg
      421 gtccaagaca ggggccgttc tgctcgaagg ctacggtgtg accgagtgct cgcctgtgct
      481 cgccgtcaat gtgcccgaga cgaacaagcc aggcacggtc ggcaagatgc tgccggcgat
      541 cgagtatcgg ctggcaccag tcgagggcct aaaaaacgct gggcggctgt cggtcaaagg
      601 ccccaacgtg atgctgggct acatgttgcc cgagaacccc ggcgtcatct cgcctccgcc
      661 cgatggttgg cacgatacgg gcgacatcgt atcgatcgac gaggaaggtt tcgtcaccat
      721 tcgcggccgc gcgaagcggt tcgcaaagat cggcggcgag atggtctcgc tcgcggctgt
      781 cgagtcggtg atcagcacgc tctggccaga gggctaccac gttgtggtct ccgcgccgga
      841 tccgcacaaa ggcgagcagc tggtgctcgt gaccgacatg gcggacgcag accgcgatac
      901 gctgatccgg cacatgcacg aggaagggct cccggccctt tgggttccga aagcgattta
      961 cgtcgtgcca gcaattccgg tccttggatc cggcaaagtc gatcttgtgg cgacgatcga
     1021 gctcgtgaag cagaagctcc catttctatg aaacgcctgc gcgcgcgtac ttctgcattt
     1081 cttcagcggc ttggcgccgg atggaccaac tctcataagc gcggtgtggc ggtgtccccg
     1141 aacgttggag ctttggccgc tgagaagcaa ggtacgctga acacctggag aaatatttgg
     1201 agagagcgcc ttgaagcggc ggtcctgacg ccgataaggg agtttcgcct cgcctatctg
     1261 ccgctgctga tgatctactt cgcctatggg gcgctcggga tcacggcggt ggccgacagc
     1321 ttctgggtga agctgtcgct gacgctaacg cctgaggacc ttgcagctct gggcgtctgg
     1381 ctgacgctgc cgtggaccgt caagatggtc ttcggcgagc tggtcgacgc cgtgccgctg
     1441 ctcggctcac agcgccgggc ctacatcttt ctcggtgccg ggatggtcgc gaccgggctc
     1501 gttctgctgg ccggggccgc gggcggctgg attactttcg cctcgccgga cacgatctat
     1561 gttctagcct cccttctgac cgtgacgggc gtcgtgctcc aggacgtcgt ggcggatgcc
     1621 atgagcacgg aggtggtcgc ccgtgagaac ccagatggta cgccacgaga caaagccgcg
     1681 gttgatcgcg acctcggtat ggtgcaggtg ctggggcggc ttgcgctttc actcggcata
     1741 ttctcggtgg cttggctggg aggctacctg gcgcaagtcc tctcctacga aaccattttc
     1801 ctgatcggcc tcgtggtgcc gctgatctcg atcaccggcg cggccctcgt gcgcttggaa
     1861 acggctgcgc cccggcccat cgactggcgt ctgcttgcgg gtggtctcgc gttcggcgtg
     1921 gttgtcacgc tgctaggggt gaccggtctg ccgttcaacc aggagatcat cttcgtcgtc
     1981 tcgataacgg tcatcatcct gatgctgaaa cgggtggtgg ccggagtcga cgacgagacg
     2041 cggaagcgca tcttctacgc cgcgctcgtg attttcgcgt tccgagccgt cccgggcacg
     2101 ggcgttggat accagtggtt cagcatcgac aggctcggct tcgacgaggc tttcttcggc
     2161 gtcttgcagc agatcggtgc tggcattggt ctggtcgcgg cttggttgct gtcggacagc
     2221 atcacgcggt atccgatcgc ccgcgtcatg ctctggctga cgatccttgg cgctatcctc
     2281 tctatcccga acctgatttt ggtccacgag ctgcatgtgt ggaccgagca ggtgctcggc
     2341 atcggcgccc gccagatcgc cattctcgat acggcagtgg agtcaccgct gctcaacatc
     2401 agcatgatcc cgatgctgac gttgatcgcg atctacgctc ccgccggcca ccgggccacg
     2461 tggttcgcgc tgatggcctc gctgatgaac ctggcgctcg tcgccgggca gctcatgacg
     2521 aagtacctca acatgctctt cgtcgtgaac cgcggggact acatcgcact gccggcgctg
     2581 acgtggagtg cggtcattct cggactagtc attccgcttt tcgtcatctt gcgc
//
LOCUS       DASUMG010000063         3277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_811755, whole genome shotgun sequence.
ACCESSION   DASUMG010000063 DASUMG010000000
VERSION     DASUMG010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811755"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1516)
                     /locus_tag="VFX46_01010"
     CDS             complement(122..1516)
                     /locus_tag="VFX46_01010"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX5599196.1"
                     /translation="MDLKTISAIDLAEAIRSKKISSTEAVSSALERLKGVHEAFNAVV
                     STDTEEALAAAKEIDDTVAAGQPLGPLAGVPLAHKDMFDRAGKIPSWGARIRREKPCT
                     EDGTLIARLKAAGSIQIAALHMTEFAFGPTGHNYVLGHARNPYDPSRIPGGSSSGSAI
                     CVATGVVPAALGSDTAGSLRLPAAACNVVSLKGTWTRVSRAGAMPLSPSLDCIGPIAR
                     DVRDLALLFGLIAGHDPRDGFSSRRSVPDYLSLIEQPIDGIRIGVDERLIGEAHPEVQ
                     QRLETSLKILEKAGARRISVRFQDWKALDTLGQLLQLPEVASAHGPYMRKRAAEYGPQ
                     VRARIEFGHFISAADHQTALRARGTMLSRVLTETFANCDMVILPTFADPLPTIAELDV
                     GGGPQLMAALGRVIFYTRPVNYLGLPALTLPYPRESYLPNGFQLVGRPFDEARMLAVG
                     RAYQKEVPGEVVTA"
     gene            complement(1521..1715)
                     /locus_tag="VFX46_01015"
     CDS             complement(1521..1715)
                     /locus_tag="VFX46_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599197.1"
                     /translation="MAETGADLPKQPKSLGEHTVPQERLDVILPFVEMLSETAVKVGD
                     TLPLEADASDIIRVLESEGN"
     gene            complement(1958..2281)
                     /gene="soxZ"
                     /locus_tag="VFX46_01020"
     CDS             complement(1958..2281)
                     /gene="soxZ"
                     /locus_tag="VFX46_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX5599198.1"
                     /translation="MSTKPRIRVPSTAKVGEVIEIKTLISHVMETGQRKDQAGNTIPR
                     NIINTFTATFNGKEVFKVDLQPGISANPYMAFHMRVPGPGDLELTWLDDSGNKVTEKV
                     TINVS"
     gene            complement(2332..2811)
                     /gene="soxY"
                     /locus_tag="VFX46_01025"
     CDS             complement(2332..2811)
                     /gene="soxY"
                     /locus_tag="VFX46_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HEX5599199.1"
                     /translation="MKEMLLQEAISRREALAGIAAVGAATALMLSHQPALAASGNGWE
                     EALKKIVGDQSPMEGGLTLELPEIAENGNTVPFSISVDSPMTDDDYVKAVHVLSTGNP
                     QPYIATFHFTPDSGKAAVSSRMRLAGTQDIVSVAELSNGKFLLARKEVKVTIGGCGG"
     gene            3004..>3277
                     /locus_tag="VFX46_01030"
     CDS             3004..>3277
                     /locus_tag="VFX46_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599200.1"
                     /translation="MRISVQGERLDRTWAEIGKSLLVVALILCNLLLVTTPGTATVSV
                     PNRTAPIVELIMVADPGCPYCARWEEEVGIAYAASEEGRFAPLVRRR"
BASE COUNT          555 a         1033 c         1058 g          631 t
ORIGIN      
        1 gaccagttct tttattgcag cctctgtcga ccgataggtc tcgcccagca gagaggccag
       61 ccttgtgtcg acactgaaga aggctggctc ggccatctaa atgcctccgt gttacaatga
      121 gttacgcagt tacaacctcc cccggcacct ccttctgata cgcccgtccg accgccagca
      181 tccgcgcctc atcgaacggc cgcccgacga gctggaaccc gttcggcaaa tagctctccc
      241 gcggataggg cagcgtgagc gctggcaggc cgagatagtt caccgggcgg gtgtagaaga
      301 tcacgcggcc gagggcggcc atgagttgcg ggccgccgcc aacatccagc tccgcgatgg
      361 tgggcagcgg atcggcgaag gtcggcagga tgaccatgtc gcaattggcg aacgtctccg
      421 tcagcacgcg cgacagcatg gtgccgcggg cgcgcaaggc cgtctgatga tccgcggccg
      481 agatgaaatg gccgaactcg atccgcgcgc ggacctgcgg gccatactcg gccgcacgct
      541 tgcgcatata agggccgtgc gccgaggcga cctcggggag ctggagcagc tggccgaggg
      601 tgtcgagcgc cttccagtcc tggaaccgca ctgaaatgcg gcgtgcgccc gctttttcca
      661 ggattttcag gctggtctcg agtcgctgct gaacttccgg gtgcgcctcg ccgatcagcc
      721 gctcgtcgac gccgatcctg atgccgtcga tcggctgctc gatgaggctg aggtagtccg
      781 gcaccgaccg gcgcgagctg aagccgtcgc gcggatcatg gccggcgatg aggccgaaga
      841 gaagcgcgag atcgcgcacg tcgcgcgcta tgggccctat gcagtcgagc gacggcgaca
      901 gcggcatggc gcccgcccgg gacacgcgtg tccaggtccc cttgagggaa acgacgttgc
      961 aggccgcagc cgggagccgc agcgagcccg ccgtgtcgga gcccagcgcg gccggcacga
     1021 cgcccgtcgc cacgcagatc gccgagccgc tggaggagcc acccgggatc cgcgacggat
     1081 catacgggtt gcgggcatgg ccgagcacgt agttgtgccc cgttgggccg aaggcgaact
     1141 cggtcatatg cagcgcggcg atctggatgc tgccggcggc tttgaggcgc gcaatcagcg
     1201 tgccgtcctc cgtgcagggc ttctcgcggc ggatgcgggc gccccagctc gggatcttgc
     1261 cggcgcggtc gaacatgtct ttgtgcgcaa gcggcacgcc ggcgagcggc cccagcggct
     1321 ggccagcggc gacggtgtcg tcgatctcct tcgctgcggc gagcgcttcc tcggtgtcgg
     1381 tcgagacgac ggcgttgaaa gcctcgtgca cgcccttcag ccgctccagc gcggagctga
     1441 cggcctcggt gctggagatc ttcttggagc gaatggcctc ggcgaggtcg atcgcggaaa
     1501 tcgtcttgag gtccatggcg ctagttcccc tcactctcca gcacacggat gatgtcggag
     1561 gcatcggcct cgagcggcag cgtatcgccg acctttaccg ccgtttccga cagcatctcg
     1621 acgaatggga gaatgacgtc cagccgctcc tgtgggacgg tgtgctcccc gaggcttttc
     1681 ggctgcttgg gtaagtcggc gccagtctcg gccatggcga tgtgtcctcc ggtaagtcct
     1741 tggctcggat cgagctcgtg ctttggccca acatgccggg attgccgccg gggtgcaatg
     1801 gctccccctt cgtaggggga tgaacgaggg ccgcttcgcg cttgcagcta gcccttgcgc
     1861 ctgcacccgt cgttttctgc ggacgcgcag atagcagata gccgccgcgc ttcgcgcgac
     1921 ggcagcaagt ccggcccggc aagcctgccg tgagatgtca ggagacgttg atcgtcacct
     1981 tctcggtgac cttgttgccg gaatcatcga gccacgtcag ctcgaggtca ccggggcccg
     2041 gcacacgcat atggaaggcc atgtagggat tggccgagat gcccggctgc aggtccacct
     2101 tgaaaacttc cttgccgttg aaggtggcgg tgaacgtgtt gatgatgttg cgaggaatgg
     2161 tgttgccggc ctgatccttg cgctgccctg tttccatgac gtgcgagatc aatgtcttga
     2221 tctcgatcac ctcacccacc ttcgcagtcg agggaaccct gatgcgcggc ttggtcgaca
     2281 tgtcgtttct tccttcccga attcgttgac ctcagacctg cggacagacg actagccgcc
     2341 gcaaccgccg atggtgacct tcacctcttt ccgtgcgagc aagaattttc cgttgctgag
     2401 ctctgcgacg ctcacgatgt cctgcgtgcc ggcgaggcgc atgcggctgg aaacggctgc
     2461 cttgccggag tccggcgtaa agtggaaggt tgcgatgtag ggctggggat tgccggtcga
     2521 gagcacgtga accgccttca cgtaatcgtc gtccgtcatt gggctatcga cggagatcga
     2581 gaagggcacc gtattgccgt tctccgcgat ctccggcaat tcgagcgtca acccgccctc
     2641 catcggggac tggtcgccca cgatcttctt gagggcctct tcccaaccgt taccgctggc
     2701 ggcaagggca ggctggtggg aaagcatcag agcggttgcc gcgccgactg cggcgatccc
     2761 tgccagcgcc tcacgccggc tgatggcttc ttgcaaaagc atttccttca tttctctatc
     2821 cttcggcacc accattccag caacctccct gaattggcta caatatcttc cctcgaccat
     2881 tcatcgcgca atcaccgcgg gagcgctcgc gcgcgctgta tctgtgccca tcgagcatgg
     2941 ggacttgctc tcacaatatt agcatatcag aatgttcgcc agcaaatccg agataggtgc
     3001 cacatgcgaa taagtgtgca gggcgagcgg ctcgatcgga cgtgggcaga gatcggaaag
     3061 tccttgctcg tagtggcgct gatcttatgc aatttgttgc tggttacgac gcccggtacg
     3121 gcaaccgttt ctgttccgaa ccgcacagcc cccatcgtcg agcttatcat ggtggccgat
     3181 ccgggctgcc cctattgcgc gcgatgggaa gaggaagttg gaatagccta cgcggccagt
     3241 gaggaaggcc gatttgctcc cctcgtacgg cgccggc
//
LOCUS       DASUMG010000064         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1132840, whole genome shotgun sequence.
ACCESSION   DASUMG010000064 DASUMG010000000
VERSION     DASUMG010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132840"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="VFX46_01035"
     CDS             complement(<1..179)
                     /locus_tag="VFX46_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX5599201.1"
                     /translation="MAQVNFEIGDLERRMRAAIDALKRELSGLRTGRASANLLDPVMV
                     NVYGQKLPLNQVGSIS"
     gene            complement(276..1007)
                     /gene="pyrH"
                     /locus_tag="VFX46_01040"
     CDS             complement(276..1007)
                     /gene="pyrH"
                     /locus_tag="VFX46_01040"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX5599202.1"
                     /translation="MSDVNSLRYKRVCLKLSGEAMSGRGAFGIDIAVVNRLARDVGEA
                     VQAGVRIVVVLGGGNIFRGLAGAAKGMDRATADYMGMLATVMNALAFHNALERQGTPA
                     RVLSAIPMPTICESYVRAKALHHLENGRVVICAAGTGNPFFTTDTTAALRAIEMNCDA
                     VAKATQVDGVYSADPRTDPTAVRYDRLTYADVLARDLKVMDGAAVALARDNGLPIIVF
                     SIEESGNLLKMLRGEARATIVQGSV"
     gene            complement(1234..>2437)
                     /locus_tag="VFX46_01045"
     CDS             complement(1234..>2437)
                     /locus_tag="VFX46_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEX5599203.1"
                     /translation="VLTLSATPIPRTLQLALSGVRELSLITTPPVDRLAVRTYISPFD
                     PVIIREALRRERYRGGQTFYVVPRIADLDEVHEFLTEAVPELRVARAHGQLSVTELEN
                     VMTAFYEGEYDVLLSTAIVESGLDVPNANTLIVHRADMFGLAQLYQLRGRVGRSKTRA
                     YAYFTTPPGQKLTEGAEKRLKVLHSLDTLGAGFSLASHDLDIRGAGNLLGEEQSGHIR
                     EVGFELYQSMLEEAIATLKGGDLGEVTEQWSPQISLGTSVLIPETYVPDLQLRLGLYR
                     RLSGLERRPEIDEFAAELVDRFGELPEEVRHLLDLMEIKGLCRQASVAQVEAGPKGAV
                     LTFRNNAFTNPEGLVDLIQRSRGKMKLQPDHRLVYKADWDQPEQRLKGVRRLIGDLAG
                     LAAKAVQT"
BASE COUNT          463 a          788 c          736 g          450 t
ORIGIN      
        1 gagatcgatc caacctggtt gagtggcaac ttctggccgt aaacattgac cataaccggg
       61 tcgagcagat tggcgctggc gcgtcccgtc ctcagcccgc tcagctcccg cttcagggca
      121 tcgatggcgg cacgcatgcg cctttccaga tcgccgattt cgaaattgac ctgagccata
      181 ccactcctcc tgccgggcgc ggcgatggcc ggcgcgtctc gccctcgccc tcaaaacggg
      241 cggacggtgc gacggggccg cgcggatcct gaagatcaga cgctaccctg gacaatggtc
      301 gcgcgggcct ctccgcgcag catcttcaga agattacccg actcctcaat tgagaaaaca
      361 attatcggaa gaccgttatc gcgggcaagc gcgacagccg cgccatccat caccttgaga
      421 tcgcgggcca acacatcggc ataggtgagc cgatcatagc gaaccgcagt tggatccgta
      481 cgcggatcgg cagagtacac accatcgacc tgagtggcct tggctacggc gtcgcagttc
      541 atctcaatgg cgcgcagcgc cgctgtggtg tctgtggtga agaagggatt tcccgttccc
      601 gccgcgcaaa tcaccacacg gccgttctcc aggtggtgca acgcctttgc ccgcacgtag
      661 gattcgcaga tggttggcat cggaattgct gacagcactc gcgccggtgt gccctgtcgc
      721 tcaagggcgt tgtggaacgc gagcgcattc atgacggtgg ccagcatacc catgtagtcg
      781 gccgtggctc tgtccatccc tttggctgcc ccggcaaggc cgcggaagat gttgccaccg
      841 ccgagcacga cgacgatcct aacgccggct tgaacggctt ctcctacgtc cctggccaga
      901 cggttaacga cagctatgtc gatgccgaat gcgccgcggc cactcatggc ctcgcccgaa
      961 agcttcaggc acacacgctt ataccgcagt gagttcacgt cgctcatcct gcccgccttc
     1021 cttcgccgcc atgtctgtta cgaccctcga cacatggtcc gaggatcctt cgagtgtgcc
     1081 gagctgaagc tgcatgaccg aacacgagcg cagcatgcct gcaacccggt atggagcatc
     1141 accgatccat gtgagccgcc gcttcggtca ttcgctcgac ggggccggcg ttccgatagc
     1201 ccgttgaagg atcagggaca agtgccagag ccgtcaggtc tgcacagcct tggctgccag
     1261 ccccgccaga tcgccaatga gccgccgcac acccttcagg cgctgctcgg gttgatccca
     1321 gtcggccttg tacacgagcc tgtggtcggg ctgcagcttc atctttcctc gtgagcgctg
     1381 gatcaggtcg accaatccct ccggattggt aaaggcattg ttgcggaagg tgagaaccgc
     1441 acccttgggg ccagcctcga cctgggcgac gctcgcctgc cggcatagcc ccttgatctc
     1501 catgagatcg agcaggtgcc gaacttcctc gggcaactcg ccgaagcggt cgacgagctc
     1561 tgcggcgaac tcatcgatct cgggccgccg ctcgagcccc gagaggcgcc ggtacagtcc
     1621 tagcctgagc tgcagatccg ggacgtaggt ctcgggaatc aataccgatg tgccgagcga
     1681 gatctgcggg ctccattgct cggttacctc gccgagatcg ccgcccttca gggtcgcgat
     1741 ggcctcctcc agcatcgact ggtaaagctc gaacccgacc tcacggatgt gccccgactg
     1801 ctcctccccg agcaggttgc cggcgccacg tatgtcgagg tcgtggctgg ccaaggaaaa
     1861 gcctgcacct agcgtatcaa gcgagtgcaa caccttaagg cgcttctccg ccccctcggt
     1921 gagtttctga ccgggtggcg ttgtgaaata cgcataggca cgagtctttg agcgcccgac
     1981 acgtccgcgg agctgataga gctgcgccaa accgaacatg tcggcgcgat gcacgatcaa
     2041 cgtattggca tttggcacat cgagaccgga ctcgacgatc gccgtcgaaa gcaacacgtc
     2101 gtactcgccc tcgtagaacg ccgtcataac gttctcgagc tcagtcacgg acagttggcc
     2161 gtgcgcgcgc gcaacccgca gctcgggaac agcttcggtt agaaactcgt gcacctcgtc
     2221 gagatctgcg atgcgcggca cgacgtagaa cgtctgaccg ccgcggtagc gttctctgcg
     2281 cagcgcctcg cggatgatga ccgggtcgaa cggcgagata tacgtgcgca ccgccagccg
     2341 atcgaccggt ggtgtggtga tgagagagag ttcgcggacg ccggagaggg caagttgaag
     2401 cgtgcgcggg atgggtgtcg cggacagcgt cagcacg
//
LOCUS       DASUMG010000065         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1133037, whole genome shotgun sequence.
ACCESSION   DASUMG010000065 DASUMG010000000
VERSION     DASUMG010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133037"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /gene="clpB"
                     /locus_tag="VFX46_01050"
     CDS             complement(<1..2231)
                     /gene="clpB"
                     /locus_tag="VFX46_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEX5599204.1"
                     /translation="MNFDRYTDRAKGFVQAAQTLALREGHQQFSPEHLLKVLLDDEEG
                     LAAGLIERAGGQSRQALVAVEQALAKRPKVSGSGAGQLYLTPELARVFDQAEQVADKA
                     GDKFVTVERLLLAIALARDTEAGRILANAGVTPQNLNQAINELRKGRTADTATAEQAY
                     DALKRYARDLTEAAAEGKLDPVIGRDEEIRRTMQILSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQRIVKGDVPESLKDKKLLALDMGALIAGSKYRGEFEERLKAVLQEVTAAEGGII
                     LFIDELHTIVGAGKSEGAMDASNLLKPALARGELHCIGATTLDEYRKHIEKDAALARR
                     FQPLFVSEPSVEDTISILRGLKERYEQHHKVRISDSALVAAATLSHRYITDRFLPDKA
                     IDLVDEAASRLRMQVDSKPEELDSIDREIVRLKIEAEALKKETDAASKDRLQKLEAEL
                     SELEERSRELTVRWQQEKDKLGRAAQLKSELDRARNELAHAQRRGEWQRAGELTYAII
                     PKLEKELAELEAKGGDGEFLEEAVTANHIAQVVSRWTGVPVDKMLEGEREKLLKMEQA
                     LSKRVVGQEEAVEAVSTAVRRARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAEFLF
                     DDESAMVRIDMSEYMEKHSVARLIGAPPGYVGYEEGGALTEAVRRRPYQVVLFDEIEK
                     AHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSNIGAEYLVSAKTEEDFETAKS
                     FVLA"
BASE COUNT          386 a          803 c          715 g          491 t
ORIGIN      
        1 gcgagtacga acgacttcgc tgtttcgaag tcttcctcgg tttttgcgga cacgaggtac
       61 tcggcaccga tgttcgaggt catgatgatc agcgtgttcc ggaagtcgac cgtgcggccc
      121 tgcccgtccg tcaggcgtcc atcatcgagc acctgcaaca gcacgttgaa gacgtccggg
      181 tgggctttct cgatctcgtc gaacagcacc acctggtagg gccgccgccg gacggcctcg
      241 gtcagagcgc cgccttcttc atagccgacg tatccggggg gagccccgat gaggcgagcc
      301 acggagtgct tctccatgta ctcgctcatg tcgatgcgta ccatcgccga ctcgtcgtca
      361 aacaggaatt cggcgagcgc cttcgtcagc tccgttttac cgacgccagt ggggccgagg
      421 aacatgaacg agccaatcgg ccggttcggg tcctgcagcc cagcccgcgc acggcggact
      481 gccgtggaca cagcctcgac cgcctcctcc tggccgacaa cccgcttgga gagcgcctgc
      541 tccatcttga ggagcttttc gcgctcacct tccagcatct tgtcgacggg cacgccggtc
      601 caacgcgaga cgacctgggc gatatggttc gcggtgaccg cctcctccag gaactcgccg
      661 tcgccgccct tcgcctcaag ctctgccaat tccttctcga gcttggggat aatggcatag
      721 gtcagctcgc ccgcacgctg ccattccccg cgccgctgag cgtgcgcgag ctcattgcgg
      781 gcacggtcga gctccgactt cagctgagct gccctgccga gcttatcctt ctcctgctgc
      841 cagcggaccg tcagctcacg cgagcgctcc tcaagctctg acagctccgc ctctagcttc
      901 tgcagccggt ccttggaggc cgcgtcagtc tccttcttaa gggcctcggc ctcgatcttc
      961 agccgtacga tctcccgatc gatggaatcg agttcttccg gctttgagtc cacctgcatt
     1021 ctgaggcgcg aggcggcctc gtcaacgagg tcgatcgcct tgtccggcaa gaaccggtcg
     1081 gtgatgtaac ggtgcgaaag cgtcgccgcg gccaccagcg ctgagtcgga aatgcgcacc
     1141 ttgtggtgct gctcgtagcg ctcctttagg ccgcgcagaa tcgagatggt gtcttccacc
     1201 gacggctcgg agacgaacag cggctggaaa cggcgggcca aagccgcgtc cttctcgatg
     1261 tgtttacggt actcgtcgag cgtggtcgcg ccgatgcagt gcagttcacc acgggccaac
     1321 gccggcttta gcaggttgga ggcatccatc gcgccttccg atttgccggc cccgacgatg
     1381 gtgtgcagct cgtcgatgaa caagatgatt ccgccctcag ccgccgtcac ctcttgcagc
     1441 accgccttga gccgctcctc gaattcaccg cggtacttcg agccggcgat caatgcgccc
     1501 atgtcgaggg caagcagctt cttgtccttg aggctctcgg ggacgtcgcc tttgacgatg
     1561 cgctgcgcga gcccctcggc gatggctgtc ttaccgacgc ccggctcgcc gatcagcacg
     1621 gggttattct ttgtccgccg ggacaatatt tgcatcgttc ggcggatttc ctcgtcgcgg
     1681 ccgataaccg ggtcaagctt accctccgcg gccgcctcgg tcaggtcgcg cgcataccgc
     1741 ttcaaggcat cataggcctg ctcggccgta gctgtgtccg cagtccggcc tttgcgcagc
     1801 tcgttgatcg cctgattgag gttctgtgga gtgaccccgg cgtttgcgag gatccgcccg
     1861 gcttcggtgt cgcgagcaag tgcgatcgcg agaagcagtc gctcgacagt aacgaatttg
     1921 tcgccagcct tgtcggcgac ctgctcggcc tgatcgaaaa cgcgggccag ttcaggtgtg
     1981 aggtaaagct gccccgcacc gctgcccgaa accttcggcc ttttcgccag tgcttgctcg
     2041 accgccacga gcgcttgcct ggattgtccg cccgcccgtt caatcagtcc ggccgccaag
     2101 ccctcctcgt catcaaggag aactttgagc aggtgctcgg gcgagaactg ttgatggcct
     2161 tcccgcagag ccaaagtctg ggccgcctgg acgaagcctt tggcccggtc cgtataccta
     2221 tcgaaattca tgtatttccc tcctcagcgc gttcgttcgc cctgctaggc acgatccgaa
     2281 cgccagtccg tcaggattgg tgacggggtc agccgggccc gacaggcacc ctccgccccc
     2341 gtcgcgtgga atatagtgtg gcgacggcgc gccggaaggg ccaggctcat gccaa
//
LOCUS       DASUMG010000066         2463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_169546, whole genome shotgun sequence.
ACCESSION   DASUMG010000066 DASUMG010000000
VERSION     DASUMG010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169546"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..>2463)
                     /locus_tag="VFX46_01055"
     CDS             complement(44..>2463)
                     /locus_tag="VFX46_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5599205.1"
                     /translation="GPTFPDPAAFFGPQFFEEFGGLSTRRLLEHAQNRLRAHQANEAD
                     DTEREEAEHKAGFISTLAAALGFAASNDEPPPEPAVELDYRDLWQRFTQASEAEIPSN
                     DQELMDVLVAALGLARDEWSDTIQLRIERLEVADDVPAIDLSVTHGGPNSSGLTFDSR
                     VFLCNRPTQGGGLKRQLDKVLSNMGGRASFMLRASDFPPNRKNQTAQAFRKFRESGGR
                     SLLVPIPDWERMLMVREFHAHHRYDPGFARWFAQAKLLSGMIPIVQLLRLDLLGQSAG
                     VLPSTTSVVRPASTPLPEPQPASNGSLRERLAKLEEDGAQPVQPASEASSAPPIPPVT
                     EKPPRWSWADWIGWGSSARTEPLPANDDMLPLSVILDENGGDGKSIYAGREIGSGKPV
                     TLNRDVLKRHAAVLGGSGSGKTTLALSLIEQLLLSGIPAVLLDRKGDLCSYANPDVWC
                     HTDNEFHDRREERKKLAESIDVAVYTPGRASGRPISITLLPNGISELPEHEQQLLANL
                     SAAALGDMLHLKNSATHQKQSGTLSVALKILGSRSRTEVTLNDLIYLLEDEDPELTDL
                     TQRMDPSGKLRRDLVAQLDSLRHRNSALFEGGGESLRMESLLGIGPYARPGRTRLSII
                     YTGFLGDNENILFWVAQFLSEALRFCQRNPNDELQAVVMFDEADLYIPANSKPATAEP
                     LQSLLKRARSAGLGLMLATQSPGDLDYKSRDQITSWFIGRVREDTALRKLKAAFQSES
                     GLDPAAVLPGQTVGEFHLVQEGVVRALKAQRSLISAEQVPFDRIEQLAYETKNQDERQ
                     LPLFEMR"
BASE COUNT          439 a          790 c          781 g          453 t
ORIGIN      
        1 ccactttggg cctaacccgg tcgggtgcta gttcgatcgc gagctatctc atctcaaaaa
       61 gcggaagctg ccgttcgtcc tggttcttcg tttcgtaggc caattgctcg atacggtcga
      121 acggcacctg ctcggcgctg atcagggacc tctgggcttt caatgcgcgc accacacctt
      181 cctgcacgag gtggaattcg ccaacggtct gccctggcag cacagcggcc ggatcgaggc
      241 cgctctcgct ctggaaagcc gccttcagct tgcgcagcgc cgtatcctcg cggacgcggc
      301 cgatgaacca ggaggtgatc tggtcgcggc tcttgtagtc caggtcgccc ggtgactgcg
      361 ttgcgagcat cagtccgagc cctgcagaac gggcgcgctt caacaggctt tgaagcggct
      421 cggcagttgc aggcttggag ttcgccggaa tgtagaggtc ggcctcgtcg aacatgacga
      481 cagcctgcag ctcgtcattc gggttgcgct ggcagaagcg aagcgcctcc gacagaaact
      541 gcgctaccca gaacaggata ttctcgttgt cgccgaggaa gcccgtgtag atgatcgaca
      601 ggcgcgtgcg ccccggtcgc gcataaggac caatgccaag cagcgactcc atgcgcaggc
      661 tctcgccacc gccctcaaac agagccgagt tgcggtggcg cagactgtcg agctgggcca
      721 ccaggtcccg ccggagcttt cccgacgggt ccatgcgctg tgtcaggtcc gtcagctccg
      781 ggtcctcgtc ctcaaggaga tagatgagat cgttgagcgt aacttcggtg cgcgaccggc
      841 tcccgagaat cttcagcgcc acggacaacg tgccggactg cttctggtgc gtggccgagt
      901 tcttgaggtg cagcatatcc ccgagcgcgg ctgcggacag gttcgccaga agctgctgct
      961 catgctccgg caactcgctg atgccattcg ggagaagcgt aatcgatatc ggcctgccag
     1021 aggcgcgccc gggcgtgtag acagccacat cgatcgattc cgcgagcttc ttgcgctcct
     1081 cgcggcgatc gtggaactcg ttatctgtat ggcaccagac gtccggattg gcgtagctgc
     1141 agagatcgcc cttgcggtcg agcagcaccg caggaattcc gctcagcagc agctgctcga
     1201 tgagactgag cgccagtgtt gtcttgcccg agccggagcc accgagcacg gcggcgtgcc
     1261 gcttgagcac gtcgcggttg agggtcaccg gcttgccgct accgatctcg cgcccagcgt
     1321 agatgctctt tccgtctccg ccgttctcgt cgaggattac cgagagcggc agcatgtcgt
     1381 cgttggccgg tagcggctcg gttcgagccg aggagcccca gccgatccag tcggcccagc
     1441 tccatcgagg cggcttctcc gtcacaggcg gaatgggagg cgcagacgac gcctcagatg
     1501 ccggctgaac cggttgcgcc ccatcctcct cgagcttcgc cagcctctca cgcagcgagc
     1561 cattgctggc cggctgcggc tccggaagtg gtgtgctcgc tgggcgtacc actgaggtgg
     1621 tcgacggcag cactcccgcg gattggccaa gaagatcgag ccgcaaaagc tgcactattg
     1681 ggatcatgcc cgacagcagc ttcgcctggg caaaccagcg tgcgaaaccg ggatcgtaac
     1741 ggtggtgggc gtggaactca cgcaccatca acatacgctc ccaatccggg atcggcacca
     1801 gcagcgagcg accaccactc tcacggaatt tgcggaatgc ctgcgcagtc tgattcttcc
     1861 ggttgggcgg gaaatcgctg gctcgcagca tgaatgaggc gcgcccgccc atgttgctca
     1921 gcaccttatc gagctggcgc ttgagtccgc ccccctgggt gggccggttg cagaggaaga
     1981 cgcgtgagtc gaacgtcagc ccgctgctgt tcgggccacc gtgggtcacc gagagatcga
     2041 tggccgggac atcgtcggcc acttccaggc gctcgatccg gagctggatc gtatcgctcc
     2101 actcgtcgcg cgcgagaccc agcgcggcga ccaagacatc catcagctcc tgatcattcg
     2161 acggaatctc cgcttccgag gcctgggtga acctctgcca gagatcgcga tagtcgagct
     2221 ccacggctgg ttcaggtgga ggctcgtcgt tgctcgcagc aaagcccaac gcggccgcga
     2281 gcgtagagat gaacccagcc ttatgttcag cctcctcgcg ctcggtgtcg tcggcctcat
     2341 tggcctggtg agcccgcaag cggttctgag cgtgctcgag cagccgccgg gtcgaaagcc
     2401 cgccgaactc ctcgaaaaac tgggggccaa aaaaggcagc cggatcgggg aacgtcggtc
     2461 ccc
//
LOCUS       DASUMG010000067         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1052980, whole genome shotgun sequence.
ACCESSION   DASUMG010000067 DASUMG010000000
VERSION     DASUMG010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052980"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1673
                     /locus_tag="VFX46_01060"
     CDS             <1..1673
                     /locus_tag="VFX46_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5599206.1"
                     /translation="RDLLTTRNVLVVDEAGVVGTRQLERVLSHAADAGAKVVLVGDPQ
                     QLQAIEAGAAFRSIHERQGGAEIGEVRRQREDWQRDATRDLATGRTGHALEAYRSHGM
                     VHEAQTREQARGDLIERWDRDRQSAPGRSRIILTHTNDEVRALNEAARERMRAARNLE
                     DEVRLTVERGERRFASGDRVMFLQNERGLGVKNGTLGTIEHVSAQSMSVQTDDGRSVS
                     FDVRDYTRIDHGYAATFHKAQGMTVDRAHVLATPGMDVHGSYVALSRHRDGVDLHYGR
                     DDFASRDRLVRTLSRDRAKDMASDYERADPTQNYAERRGITFRERVAEIVRKVVSDRV
                     RNIFDGLRPSAEAVPGTGGARMPQPEAPERKAVEEPEWVALRKARTNALSRHARAVNA
                     IFDMQDQGSKASPEQVKELQEARKVFEEVRPYGSHDAEAAYKKNPELALEAASGNSAR
                     AIRALQLETELRTDPGRRADRFVERWQKLDHTGQRQYQGGDIAGYKATRSAMGDMARS
                     LERDPQLESILANRKQELGIAFESGRRLGQELAFTHGLNIGRGRGIGI"
     gene            1801..2013
                     /locus_tag="VFX46_01065"
     CDS             1801..2013
                     /locus_tag="VFX46_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HEX5599207.1"
                     /translation="MREPDRIIRLRTVLSRTGLSRSTIYRKIAEGTFPPQIKISINGS
                     GWHESDINRWIADPVAWRPKREFDAI"
     gene            complement(2617..2695)
                     /locus_tag="VFX46_01070"
     tRNA            complement(2617..2695)
                     /locus_tag="VFX46_01070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2635..2637),aa:Leu,seq:caa)
BASE COUNT          577 a          888 c          889 g          483 t
ORIGIN      
        1 atcgcgacct gctcacgacc cgcaatgtgc ttgtcgtcga cgaggccggc gtggtcggca
       61 cccgacagct ggaacgcgtg ctgtcccatg ctgccgacgc cggggccaag gtcgtcctgg
      121 tcggcgatcc gcaacaactg caagccatcg aggcgggcgc tgccttccgc tcgattcacg
      181 aacgacaagg gggcgcggaa atcggcgagg tgcgccgcca gcgtgaagac tggcagcgcg
      241 acgccacacg cgatctcgcg acgggcagaa ccggccatgc gctggaagcc tatcgctccc
      301 acggcatggt gcatgaggcg caaacacgcg agcaggcgcg gggcgatctg atcgagcgct
      361 gggaccgcga ccggcagtca gcgccggggc gcagccggat catcctcaca cacaccaacg
      421 acgaggtgcg cgccctcaat gaggccgcgc gggagcggat gcgggctgcg cgtaatttgg
      481 aggatgaggt gcgcctcacg gtcgagcgcg gcgagcgccg cttcgcgagc ggggatcgcg
      541 tcatgttcct gcaaaacgag cgcgggctcg gcgtaaagaa cggtacgctg ggaaccatcg
      601 aacatgtcag cgcgcaatcc atgtcggtgc agaccgacga tggccgatcc gttagtttcg
      661 acgtcaggga ttatacccgg atcgaccatg gctatgccgc gaccttccac aaggctcagg
      721 gcatgacggt ggaccgcgcc catgtgctgg cgaccccagg catggacgtc cacggcagct
      781 atgtcgccct gtcgcggcac cgcgacggcg tggacctgca ttatggccgt gacgattttg
      841 ccagtcggga ccggcttgtc cgcaccctgt cgcgcgaccg ggccaaagac atggcttcgg
      901 attacgaacg cgccgatccg acccaaaact atgccgagcg gcgtgggatc accttccgcg
      961 agcgtgtggc cgagattgtg cgcaaggtcg tgtccgatag ggtgcgcaac atattcgatg
     1021 gcctgcgccc gtctgccgaa gctgtgcctg gcacaggcgg cgcgcggatg ccgcaaccgg
     1081 aagcgccgga aaggaaggcg gtggaagaac cggaatgggt ggcgctgcgt aaggcccgca
     1141 cgaatgcgct cagccgtcat gcccgagccg tgaatgcgat cttcgatatg caggaccagg
     1201 gcagcaaggc cagcccggag caggtgaagg aattgcagga ggcccgcaag gtcttcgagg
     1261 aggtgcggcc ctatggctcg cacgacgccg aagctgccta taagaaaaac ccggaactcg
     1321 cccttgaagc cgcttcgggc aactctgccc gcgccatccg cgcgcttcag ctcgaaaccg
     1381 aactgcgcac cgatccgggc cgccgcgccg accgtttcgt ggaacgctgg cagaagctcg
     1441 accacaccgg ccagcgccag tatcagggcg gcgacatcgc cggctacaag gcgacgcgct
     1501 cggcgatggg cgacatggcg agaagcctcg aacgcgatcc gcaactggaa tccatcctcg
     1561 ccaaccgcaa gcaggaactc ggcatcgcat ttgaatcagg ccgccggctc ggccaggaat
     1621 tggccttcac tcacggcctc aacattggca gaggccgggg catcggaatt tgatccatcc
     1681 atttgccgcc gtctctacgc cacgctgcga tgagtcgaac gaccctcttg cccgctgtaa
     1741 ctgcttgtcc tgtctggtct cagaagccgt cagagacagc cagcaggagg cagcgcggtt
     1801 atgcgcgaac cagaccgtat catccgcttg agaaccgtgc tgtcccggac cgggttgtcc
     1861 cggtccacaa tctaccgcaa gatcgccgag ggcacgttcc cgccgcagat caagatcagc
     1921 attaacggga gcggctggca tgagtccgac atcaaccgct ggatcgccga tcccgtcgca
     1981 tggcgtccga aacgcgagtt cgatgcgatc tgagaggcct cgggtggatt gctgccgata
     2041 acagtgaacc ggaccggcgt ctggccgtca aggctggcgc tgtcgcgccc ccgcttcgcg
     2101 gcttcggcct tgacggcctg acgccgtccc ggtttgagct ctccaagcag acaaaggcgg
     2161 attgggagcg gccgcgcttc caatctagaa cgaagaggca gccgacggag ctcgacggca
     2221 aaaaacttcc ccagactaaa ccccagacta aacgcaaatt ttagtctggg cgcattttag
     2281 gccgttctga aaatcgacga catgtgaaga agatagctgg cgcgcccgaa gagattcgaa
     2341 ctcctgaccc ccagattcgt agtctaacgg tgaggcagcg aagccggcgc agcaaagatc
     2401 gtgaaccgcc acgcgagccg tgatctgacg tgagttgcgg cgctcgccgt cgacctgcga
     2461 tcacaattga gtcatcgctt cccttccgcc cttgccgatt tttcgctgcg actttcgtcc
     2521 tgccgcttcc acggcgcttc cacgagcgaa gtttccagtc aaattgggag cagcaaaaag
     2581 ccctcgaaaa cgatgcgttc tcaagggctt aagacttggt tgcggggata ggatttgaac
     2641 ctatgacctt caggttatga gcctggctag cgagctaggc ggtccatcct acgcggcgcc
     2701 ccttccaatt tcgtcacctt gaaatcaaga tttgcctgcc gcgcgtattg gccggaaata
     2761 ttcggtgccg ccaggcacgt gatcgcattg tttgtcgtac gtccgggcaa atagcattct
     2821 gaaaagttat cggatct
//
LOCUS       DASUMG010000068         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_892628, whole genome shotgun sequence.
ACCESSION   DASUMG010000068 DASUMG010000000
VERSION     DASUMG010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892628"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..1483)
                     /locus_tag="VFX46_01075"
     CDS             complement(188..1483)
                     /locus_tag="VFX46_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599208.1"
                     /translation="MAGLLRPVTALLIAAAILLMGNGLLNVLIPVRADVEQFSRLEIG
                     LLGSAYYAGLVAGCLLRPLVIARVGHIRSFVAFTAIATITPLLHAILTEPISWSALRA
                     LNGLCFAGLFMGIESWLSGASTMETRGRVLASYTVLNLTVVTLGMQMMALGSPSGFQL
                     FSLVAILYSLAAVPVALTRTSAPVPPKTAKLRLAWLIGVSPAAVAGCFFNGVANGAFW
                     ALAPLYGRSAGFSVAEVAMFMALAVLGGAASQWPIGYLSDRVGRRPLLVILGVSGAIA
                     GFGLYVLSTSSPTAILPIAVLWGCSAFPIYTLCVAHANDLVHKKRAVEVSSGLLLTFS
                     IGAVLGPLIAGYTMSVAGHGALFLHTATAHLLITLVMLIRAKLRPKLPAEHKEGFVAV
                     PKTTPLVFELDPRAEGATSCGNDVVTEPSAQDLAAEQRA"
     gene            complement(1529..1939)
                     /locus_tag="VFX46_01080"
     CDS             complement(1529..1939)
                     /locus_tag="VFX46_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5599209.1"
                     /translation="MPPPKTPLLTVDCVVLNPRGEVLLIQRKNEPFKGHFALPGGFVD
                     IGETVEDACRRELLEETGVKAGRLKLVGVYSDPNRDPRGHTASVVFLTRVSSTKLEAG
                     DDAASAQWVANWRRLPLAFDHAKILADATRMAKS"
     gene            complement(2048..>3039)
                     /locus_tag="VFX46_01085"
     CDS             complement(2048..>3039)
                     /locus_tag="VFX46_01085"
                     /inference="COORDINATES: protein motif:HMM:NF042682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HEX5599210.1"
                     /translation="KLVVPGDRLLQAIADRVRKGPFHTLDAHDYAVKLFGDSIAANMF
                     LLGYAYQLGFVPIGAAAIEQAIELNGAAVGMNRAAFRFGRLTAHDKAAVDRVVKLQDE
                     HAGEPETLDEIIALRARHLTDYQDEELASRYRSKVAWIAGVEKAKAPGSTGLALAVAR
                     AYHKLLAYKDEYEVARLFAVEAFQREINEQFEGVRAMEFYLAPPILSRFFKDKTTGHP
                     RKIRLGSWMLPVFRLLAKGKKLRGTKWDVFGYSAERKLERQMIADYERLLDVIASRLD
                     QSNYETAVALAELPLEIRGFGHVKMANYKAAKSREADLLQAFLNPAPVKVAAE"
BASE COUNT          626 a          885 c          947 g          581 t
ORIGIN      
        1 gagccggcgg cgcggaaccg ctcttcgaat gccgcgtaga gatttccttc gatcgccatg
       61 agctttgccg ttttggtttg gagtgttcag gtcaccagcc gagggcaggc ggataaaacg
      121 tcttttggct gcgataggca aggccggggc atcaatagaa gtcgctttga ggccctgggc
      181 atctggatca cgcccgctgt tcggcggcga ggtcctgagc cgaaggctcg gttacgacgt
      241 cgttcccaca tgaagtggca ccctccgcac gcggatcgag ctcgaaaacg agcggggtgg
      301 tcttgggcac tgcgacgaag ccttccttat gttctgccgg caacttcggt cgcagtttgg
      361 cccggatcag catgacgagc gtaatcagga ggtgcgctgt tgccgtgtgc aggaacagtg
      421 cgccatggcc cgctacgctc atggtgtagc cggcaatgag gggaccaagc acagcgccga
      481 tcgagaaggt gagcagcagg ccgcttgaaa cctccacggc gcgcttcttg tgcaccagat
      541 cgttcgcgtg tgccacgcag agggtgtaga tcgggaacgc actgcagccc cagagcaccg
      601 caatcgggag gatggcggtc ggactgctgg tgctcagcac gtaaagtcca aaccctgcaa
      661 tggccccaga gacgccgagg atcaccaaca gcggccgccg gccgacccgg tccgaaaggt
      721 agccgatcgg ccactggctg gcggcaccgc cgagaactgc caaggccatg aacatcgcga
      781 cctcggctac ggagaagcct gcgctgcggc cgtaaagtgg ggcgagtgcc cagaatgcgc
      841 cgtttgcgac tccattgaag aaacagccgg caacagctgc cggtgagaca ccaatgagcc
      901 aggccagccg caacttggcg gttttaggcg gaacgggtgc gcttgttctg gtgagcgcaa
      961 caggtacggc tgcgagagag taaaggatcg cgaccaatga gaacagttgg aaaccgctgg
     1021 ggcttccaag tgccatcatc tgcatcccca gcgtgaccac tgtcagattg aggaccgtgt
     1081 atgacgcgag cacgcggcct cgcgtctcca tggtcgatgc gccgctcagc cagctctcga
     1141 tgcccatgaa caggccagcg aagcagaggc cgttgagtgc ccggagtgct gaccaggaaa
     1201 taggctctgt caagatcgca tgcagtagtg gcgtgatcgt cgcgatggca gtgaacgcca
     1261 caaaggagcg gatatggccg acgcgagcga tcaccaaagg tctgagcaaa cagcctgcaa
     1321 ctagaccggc gtaataggca gatccgagga gaccgatttc aagccgggaa aactgctcga
     1381 cgtcggcccg cactggaatg agcacgttga gcagaccgtt ccccatcaga agaatggccg
     1441 cggctatgag aagtgcggtt acgggtctga gaaggccggc catgtccctg cggtggtctg
     1501 cgcaatgtgc ggtgttgagg caactcgatc agcttttagc catacgagtc gcatccgcca
     1561 ggattttggc gtgatcgaag gccagcggca atctgcgcca gtttgcgacc cactgggcgg
     1621 atgccgcgtc gtcccccgcc tcaagcttag tggagcttac acgtgtgagg aacacgacgg
     1681 aagctgtgtg accgcgggga tcgcgattgg gatccgagta gacgccgaca agttttagcc
     1741 gaccggcttt gacgccggtt tcctccagca gctcccgccg gcaggcgtct tctaccgtct
     1801 ctccaatgtc tacgaagcct ccgggaagag cgaagtggcc tttgaacggc tcattcttgc
     1861 gctgaataag gagcacctcg ccacgggggt tgagaacgac gcagtcgacc gtcagcaacg
     1921 gcgttttggg aggaggcacg ggcgatctcc aaggcttggg ttatcgcgcc aatattcacc
     1981 cgatgtcctt gggagagaaa agcggctgcc gatgtgtatt tttccgctgc cgtagagttc
     2041 gcgtgagcta ctctgctgcc accttcactg gagccgggtt gaggaaagcc tgcagcagat
     2101 cggcctcgcg ggatttcgca gctttgtagt tcgccatttt cacgtggccg aagcctctga
     2161 tttctaaagg caactcggcc aaagctacgg ctgtctcgta attgctttgg tcaagccggc
     2221 tagcaataac gtccagcaac cgctcatagt cggcaatcat ctggcgttcg agcttgcgct
     2281 ccgctgaata gccgaacaca tcccattttg ttccgcggag cttcttgccc ttggccagca
     2341 gccgaaacac gggaagcatc cagcttccaa ggcgaatctt tcgcggatgg cccgtggtct
     2401 tgtccttgaa gaaacgggag agaatcggcg gggcgagata gaactccatc gcccgcacac
     2461 cttcgaactg ctcattgatc tcgcgctgga atgcttcgac ggcaaacaat cgcgcgactt
     2521 cgtattcgtc cttgtaggcc aatagcttgt ggtaagcgcg tgcgacggcg agcgccaatc
     2581 cggtgctgcc aggagctttg gctttttcca ctccggcgat ccaagcaacc tttgagcggt
     2641 accgcgacgc caactcctcg tcctggtagt cggtgaggtg gcgggcgcga agggcaatga
     2701 tctcgtcgag cgtctcaggc tcgcccgcgt gctcatcctg cagcttcacc acccgatcaa
     2761 cggctgcctt gtcgtgagca gtgaggcgac cgaagcggaa cgccgcgcga ttcattccga
     2821 cagcggcgcc attcaattca attgcctgct cgatggcggc agccccaatc ggcacgaagc
     2881 cgagttggta cgcatagccc agcaggaaca tgttggcggc aatcgagtcg ccgaagagtt
     2941 tcaccgcgta gtcgtgcgca tccagggtgt gaaacggccc tttgcgcacc cgatctgcaa
     3001 tggcttgcaa cagccggtcc cccggcacta cgagtttcg
//
LOCUS       DASUMG010000069         3778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_250158, whole genome shotgun sequence.
ACCESSION   DASUMG010000069 DASUMG010000000
VERSION     DASUMG010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250158"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VFX46_01090"
     CDS             <1..662
                     /locus_tag="VFX46_01090"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5599211.1"
                     /translation="DVPNSVKSRITFTGHLRRSFPRFSNVGSLDLPKEFILVTAGGGG
                     DGGPLMQQVLAAREFDSSLNFPLVMILGPFMAADQREEIRERARRLSNIIVRDFESQI
                     ESIMMRASAIVSMGGYNTFCEILSFDKRALLVPRCRPRREQLIRATRAAELGLVDVLD
                     PLAAADPKVMAEALRRLPERPLPSSLQGRINLDGLETVCGAVGDYINDVTCPLPAYAA
                     M"
     gene            669..1946
                     /locus_tag="VFX46_01095"
                     /pseudo
     CDS             669..1946
                     /locus_tag="VFX46_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            1943..3106
                     /locus_tag="VFX46_01100"
     CDS             1943..3106
                     /locus_tag="VFX46_01100"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5599212.1"
                     /translation="MTGWRVFFYVQHLLGVGHLFRAARIAQGLHKLGFSVELVLGGPP
                     VSQLDFSGLNVIQLPPLKAGPNGFTDLITSSGEPASPAIKARRTEALLAAFERSCPDV
                     LLIESFPFGRRQMRFELLPLLEAAQRKKPRPCIACSIRDILQENRDMARAQEVVEILR
                     DYFDLVLVHGDPRLVKLDATFPLADHIESMIAYSGIVAPGSPFYYQAGSKADVIVSAG
                     GGAVGYKLLETTLLAKAKTSLAFARWLALTGGNAKPDEFERLQQLGAMNGVVVERSVP
                     DLAAALAGAQLSISQAGYNTVADILVAGCRAVFVPFAQNGETEQSRRAALLAEHGWGV
                     VVPEQELTTDALSKAIDRALSLPKPMQEFDLNGAASTAQFVRDWLTNNRRRLC"
     gene            complement(3150..>3778)
                     /locus_tag="VFX46_01105"
     CDS             complement(3150..>3778)
                     /locus_tag="VFX46_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HEX5599213.1"
                     /translation="HVGGAYGDRASSNARWVKTWPTLPEPVRRRLVLEHDDLRFSASD
                     TLWIHQHTGVRLVFDHQHFWCLNPDRLDMRQTLVRMLGTWPKGVRPKVHFSSPRTELR
                     ELKRRDPRTRKVTTVKLAPTWTGHADFCHPFEFITFLRLARGLEFDVMLEAKAKDLAL
                     IRLRADLLNYAPDIANRFGLDTAQASIIEREASEVLEEAKADDTSSRF"
BASE COUNT          740 a         1055 c         1129 g          854 t
ORIGIN      
        1 tcgacgttcc aaattctgta aagagccgca tcacgttcac aggacatttg agacgcagct
       61 ttccacgatt ttccaacgtg ggatcgttgg atttgccgaa ggagttcatc ctcgtgaccg
      121 ctggaggcgg aggcgatggc ggtccgctga tgcagcaggt gcttgcggcg cgcgagttcg
      181 actcctcgct gaattttccc ttggtcatga ttttgggacc attcatggct gccgatcagc
      241 gggaggaaat tcgggagcgt gcccgcaggc tttcaaacat tatcgtgcgc gatttcgaga
      301 gccagatcga gagcataatg atgcgcgcga gcgccattgt gagcatgggt ggctacaaca
      361 cgttctgcga aatcctttcg ttcgataagc gggcgctgct ggtgcctcgt tgccgtcccc
      421 gtcgtgagca gctcattcgc gcgactcgag cagcggagct cggccttgtc gatgtcctcg
      481 atccgttagc ggcggccgac cctaaggtca tggcggaggc tttacggcga ttgccagagc
      541 gtccgctgcc gtcttcgctg caggggcgca tcaatcttga tggtctggag acagtctgcg
      601 gtgcagtggg cgattacatc aatgatgtga cctgcccgct tccggcttat gcggcgatgt
      661 aacggaaaat gcccgtgcac cacggaaaga tcgtcgtggt cgtgaagggc tatcccagac
      721 tgtcggaaac attcattgca caagagttac tggggctgga gcgcgccgga cttcagctgc
      781 acatcgtttc gatgcgccgg ccgacggatc gcgaggcgca tccggtgcac cgtcaaatct
      841 ctgctcccgt gctctatctt cccgaatatt tttgtcatgc accattgcgg gtcattcgta
      901 gcctgatccg ggcgaggcgt ttgccaggat ttcgccaggc tttttcggcg tggctagctg
      961 atcttcggcg cgatttcagc gcgaaccgtt ttcggcgatt tggccaggct gcggttctcg
     1021 ccatggagat gccgttgggc gcgaggtggc tctaggccca tttcattcat acgcccgcgg
     1081 cagtgacccg atatgcgagc ctcatgaggg ggctgccgtg gagctgctcg gcacacgcga
     1141 aggatatttg gacatccccg gattgggagc tgcgcgataa tctcgcctca gcaagatggg
     1201 tagtgacatg cactaaagcc gggcacgagc ggctcgcgaa gcttgcgccg cgaagagacc
     1261 acgtaaagct cgtttatcac ggcctagact ttcagcgttt tgctgcccca gatctcccac
     1321 catcgcgccg ggatggaacg aatgcatctg atccggcgcg attgttggcg gtcggccgtc
     1381 tggtcgaaaa gaagggtttc gacgtcctgc tgaatgcgct ggcgcaactt ccaccagatg
     1441 tgcactggca ccttacgcac attggaggag gcaatctttc taaacagctc cagaagcagg
     1501 cacggaagct tggaatcgcg tcacggataa cttggtgtgg cccgcaaacg caatctgttg
     1561 tcctcgaggc ttaccgccgc gccgacatat ttgtgttgcc ctgccgtcag gcgaccaatg
     1621 gcgaccgcga tggattgccg aatgttctga tggaagcgca gagccagcgg ctggcctgca
     1681 tttcctctct ggtgggtggt gtgccggaga taatccgaga cggagagacg ggactgcttg
     1741 tgccaccgaa tgacagcgtg cgccttgccg aagcgttgat gaagctcatt cgcgatcctg
     1801 acctacgaaa tcgattgggt tcggcagccg agcagcacgt tagggctcat ttcgatatgc
     1861 accggggact tcagacgctc gtcagatttt ttgaggaggc gctggagggg gcgagcgaac
     1921 gacgcatgac ggaagcggcg cagtgactgg atggcgcgtc ttcttttacg tgcagcacct
     1981 gcttggcgtt gggcatctgt tccgcgccgc ccggatcgcg caagggttgc ataaattggg
     2041 cttttccgtt gagcttgtgc tgggcggtcc gcctgtttct caattggatt tctctggtct
     2101 gaatgtgatc cagcttcccc cactaaaggc tggtccgaat ggatttacgg acctgattac
     2161 cagtagcggc gaaccggcct cccccgcgat caaggctcgc cgcacagagg cgctgcttgc
     2221 cgctttcgaa cgaagctgtc ctgacgtgct cttgatcgaa tcatttccgt tcggccggcg
     2281 gcaaatgcgc ttcgaattgc tgccattgct cgaagcggct caacgcaaaa agccgcgccc
     2341 ctgcatcgct tgttccatcc gtgatattct acaagagaac agagatatgg cgcgtgctca
     2401 ggaggtcgtg gaaatcctac gcgactactt cgaccttgtc ctcgtgcatg gcgatccgcg
     2461 actcgtaaag ctcgacgcga ccttccccct cgccgatcat attgagtcga tgatcgctta
     2521 cagcgggatc gtcgctcccg gttctccatt ctactatcag gccggatcga aggctgacgt
     2581 tatcgtttcg gcaggcggcg gcgctgtggg atacaagctg ctggaaacga cacttttggc
     2641 caaagccaag acctcgctgg catttgcgcg ctggctcgcg ctcaccggtg gcaacgccaa
     2701 gccagatgaa ttcgagcgcc tccagcagct cggcgctatg aacggcgttg ttgttgagcg
     2761 ctctgttccc gatctggcgg cagcattggc gggtgcccag ctctccattt cccaagccgg
     2821 ctataatacg gttgcagata ttctcgtggc tggttgcaga gcagttttcg ttccatttgc
     2881 ccagaacgga gaaactgagc aaagccgccg tgctgctctg cttgctgagc acggatgggg
     2941 agtcgtcgtt cctgagcagg agcttacgac cgatgcgctc tccaaagcga tcgaccgtgc
     3001 gctttcgctt ccgaagccaa tgcaagaatt cgatcttaat ggcgccgcga gtaccgctca
     3061 gtttgtacgc gattggctaa ctaacaatcg caggcgcttg tgctgaaaac gaagtcggaa
     3121 cgcaaggttt ccggcgataa ttcgttggct cagaaacggc tggaagtgtc gtcggctttc
     3181 gcttcttcga gaacttctga cgcctcccgc tcaattatcg aggcttgggc agtatcgagt
     3241 ccaaaccgat ttgcaatgtc cggcgcataa ttgagcaagt cggctcgtag acgaataagg
     3301 gcaaggtcct tggccttcgc ttccagcatc acatcgaatt cgagccctcg ggcaagacgc
     3361 aagaacgtga tgaactcgaa gggatggcaa aagtcagcat gacccgtcca cgtcggtgcg
     3421 agttttaccg tggtgacttt gcgcgtcctc ggatcgcgac gcttcaattc tctgagctcc
     3481 gtgcggggag aggagaaatg gactttcggc cttacgcctt tcggccacgt gccaagcatg
     3541 cgaaccagcg tttgccgcat gtccaggcga tccggattga gacaccagaa atgctgatga
     3601 tcaaaaacga gacgcactcc ggtgtgctga tgaatccaga gcgtatcgga cgcactgaag
     3661 cgcaaatcgt cgtgctctag gacgagccgg cgacgcacag gttccggtag tgtgggccac
     3721 gtcttgaccc aacgtgcatt gcttgaagct cggtctccgt acgctccacc gacgtgaa
//
LOCUS       DASUMG010000070         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1053597, whole genome shotgun sequence.
ACCESSION   DASUMG010000070 DASUMG010000000
VERSION     DASUMG010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1053597"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VFX46_01110"
     CDS             <1..681
                     /locus_tag="VFX46_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599214.1"
                     /translation="DDSAVAQSIELMLKSESFNVYTTDLGEEGVDLGKLYDYDIILLD
                     LNLPDMSGYDVLKQLRVSKIKTPILILSGLAGIEDKVKGLGVGADDYMTKPFHKDELV
                     ARIHAIVRRSKGHAQSVIQTGDLVVNLDTKTVEVGGQRVHLTGKEYQMLELLSLRKGT
                     TLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLANASEGRNFIETVWGRGYVLREPH
                     EADERIPA"
     gene            849..1253
                     /locus_tag="VFX46_01115"
     CDS             849..1253
                     /locus_tag="VFX46_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX5599215.1"
                     /translation="MVLQSLAGLPAFLVYFCTAIVAVVAYLFVYTRVTPHNEFELIRA
                     NEPAAAIALGLSLLGFVLPLVSAIAHSANVWDCLIWAAIALIVQIIVYFLVKVPVPKL
                     SERIASGELAPAIWLGLSSLAAGALNAACMIY"
     gene            1256..1648
                     /locus_tag="VFX46_01120"
     CDS             1256..1648
                     /locus_tag="VFX46_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599216.1"
                     /translation="MANQSDHEFGKRKPVSVPASSRSGPPAKRSGHVALLLMGTVAVG
                     SAAYTLMPSRNCEPSPNAASPTSTECTSRSSSSSSHSGSGGSSSRSSYYSSDSSSSHS
                     SSTSDSGSHSAQRGGFGSFGHAVGFSGG"
     gene            1653..>2060
                     /locus_tag="VFX46_01125"
     CDS             1653..>2060
                     /locus_tag="VFX46_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HEX5599217.1"
                     /translation="MQRIPCPERDDWRATAESIGFTFHTIDGERYWDERAYYAFTLNE
                     IERRIEGPTGEIEAMCVELVSRTIGDEKYLRRLKIPEAFWPLLSESWRRRDASLYGRL
                     DLSYDGWSPAKLLEYNADTPTSIFEAAVFQWTWL"
BASE COUNT          385 a          683 c          606 g          386 t
ORIGIN      
        1 gatgatagcg ctgttgcgca gtcgatcgag ctgatgctca aatccgagag ctttaacgtc
       61 tacacgacgg atctcgggga agagggtgtc gacctcggta agctatatga ctacgacatt
      121 atccttctcg accttaacct gcccgacatg tccggttacg acgtgctcaa gcagcttcgg
      181 gtctccaaga tcaagacccc cattctgatc ctctccggcc tcgccggtat cgaggataag
      241 gtcaagggcc tcggcgtcgg cgccgatgac tacatgacca agcctttcca caaggacgaa
      301 ctggttgccc gtatccacgc gatcgtgcgc cgttccaagg gtcatgccca gtcggtcatc
      361 cagaccggcg acctcgtcgt caacctcgac accaagacgg tcgaagtcgg cggccagcgc
      421 gtccacctga cgggcaagga ataccagatg ctggagctgc tctcgctccg caagggtacg
      481 accctgacca aggaaatgtt cctcaaccac ctctatggcg gcatggacga gcccgagctg
      541 aagatcatcg acgtcttcat ctgcaagctg cgcaagaagc tggccaacgc ctccgaaggc
      601 cggaacttca tcgaaaccgt gtggggccgc ggctacgtgc tgcgcgagcc gcacgaggcg
      661 gacgagcgca tccccgcctg agccaagggt ttacggcgag ccgcaaggct cctcctcccg
      721 actggacccc gcccgaaacg gcggggtttt tgtttggggg agttttattg ggattgaacc
      781 cgacgccccg atgcgccgac tgacagatcg tgcgaacctt tcggaacgca cgagtcgggg
      841 cgaattcaat ggttttgcaa tcccttgccg gactgccggc attcctggtc tatttctgca
      901 cggcaatcgt cgccgtggtg gcctacctct tcgtctatac ccgcgtcacg ccgcataacg
      961 agttcgagtt gatccgcgcc aacgagccgg cagccgccat tgcgctgggc ctgagcctgc
     1021 tcggcttcgt gctgccgctg gtcagcgcca tcgctcattc cgccaacgtc tgggattgcc
     1081 tgatctgggc ggcaatcgcg ctgatcgtac agatcatcgt ctacttcctc gtcaaggttc
     1141 cggtgccgaa actgtcggag cggatcgcat ccggcgaact ggcccctgcg atctggctcg
     1201 ggctgtcctc gctggccgcc ggcgccctca acgccgcctg catgatctac tgaccatggc
     1261 caaccagtca gaccacgaat tcggcaagcg caaaccggtc tcggtgccgg catcgtcacg
     1321 ctccggtccg ccggcgaaac gttcgggcca cgttgcgctg ctgctgatgg gcacggttgc
     1381 ggtcggcagt gccgcctaca cgctgatgcc gagccgcaat tgcgagccat cgccgaatgc
     1441 ggcttcaccg accagcaccg agtgcacgtc gcggagctct tcatccagca gccacagcgg
     1501 ctccggcggc agttcgtcgc gcagcagcta ctacagcagc gattcgtcct cgagccattc
     1561 ctcgagcact tccgattccg gctcgcacag cgcgcagcgc ggcggcttcg gctcgttcgg
     1621 gcacgccgtc ggcttctccg gcggctgaat tcatgcagcg tatcccctgc cccgaacgcg
     1681 acgactggcg tgccaccgcc gaaagcatcg ggttcacctt tcacaccatc gacggcgagc
     1741 gctattggga cgaacgggcc tattatgcgt tcacgctgaa cgagatcgaa cggcggatcg
     1801 aagggcccac cggcgagatc gaagcgatgt gcgtcgaact ggtcagccgc acgatcggcg
     1861 acgaaaaata cctccgccgg ttgaaaatac ccgaagcctt ctggccgctg ctatccgaga
     1921 gctggcgccg ccgcgacgcc agcctctacg gccggctgga cctgagctac gacggctgga
     1981 gcccggcgaa actgctggag tacaacgcgg atacgccgac ctcgattttc gaagccgcgg
     2041 tttttcagtg gacctggctc
//
LOCUS       DASUMG010000071         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_89971, whole genome shotgun sequence.
ACCESSION   DASUMG010000071 DASUMG010000000
VERSION     DASUMG010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89971"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFX46_01130"
     CDS             <1..175
                     /locus_tag="VFX46_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5599218.1"
                     /translation="LAASIRTVNHVKHAAMIGADVVTVPPAVLQSLVKHPLTDIGLRQ
                     FVADWEKTGQKII"
     gene            290..1480
                     /locus_tag="VFX46_01135"
     CDS             290..1480
                     /locus_tag="VFX46_01135"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX5599219.1"
                     /translation="MGDFQPLRGIRVVEMSHMIMGPSCGMFLGFLGAEVIKIEPPQGD
                     KTRYLSGMGSPFFPLFNRGKKSVQLDTRTEAGREALHKLLATADVFVENFRDDSLAKM
                     GADLAELRARYPKLIIVSHKGFLTGPYQHRTALDEVVQMMTGLAYMTGPSGRPLRVGS
                     SANDIMGGLFGALSVVSALLERQSTNKGRNIRVGLFENCLLLVAQHMVQYELEGVNPP
                     PMPERDFSWPVYDIFTTADGKQLFVGAVTEGQWATLCDILGLTELKSDPRLKTKMDMI
                     NARSWTLPIVERAIAGRQSADLISAFEEAGIPFAPIAKPSDMFQDPHVMRPGGLATSR
                     MPDGKTFRAPSLPFEVDGQMMTGGGDVASVGQHTEEILGQLGLDAEMIAAARGAASGK
                     SAKA"
     gene            1533..2519
                     /gene="dctP"
                     /locus_tag="VFX46_01140"
     CDS             1533..2519
                     /gene="dctP"
                     /locus_tag="VFX46_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5599220.1"
                     /translation="MSKFKSLVAAIAIAMVGVPGVVEAATKMRCSHQLPPAHHVAQVI
                     ERWAEEVRTASEGELDVQVFGSDSLVKANQNITAVAKGDIECAFSLNFQWGRTLPIMN
                     VTVAPFAFGDLEIWRKWPDSEAAKFLEEKLLEKGVRNIAWLFQTNTSVFTSKGRFLVK
                     PEDFKGVKLRGLNAAFDAALAALGATPTSLPGSEVYQALATGVLDAGLTDVAAAVSRK
                     YYEVQDHFTVVPVISAYLHGYVNPKWYDGLSDKSKAALESAGKKAALWAVEASEKAGA
                     AAPDQLREKGVKVHIATPEEIKALEAIMRPAFDEKFATDPDAQKLIELIGKI"
     gene            2521..3066
                     /locus_tag="VFX46_01145"
     CDS             2521..3066
                     /locus_tag="VFX46_01145"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5599221.1"
                     /translation="MEGLEQGQDNQPTAEEVQPSSLLARVASALAWLGGALSALLVLI
                     ILGLVTYAVVQRYVFDRPLVWSDPVAGYLVVALMLGAAEALRRGDHISIDLLSNQLGA
                     RGRRAVAYFSDLAVLAFAVVLGWSCWEQISFAYDFGSFTADHLEIPSWIPMAPLLAAC
                     VLLALMAAARLLDALARGAQR"
BASE COUNT          596 a          895 c          985 g          641 t
ORIGIN      
        1 tctcgccgcc tctatccgca cggtgaatca cgtcaagcac gcggccatga ttggcgctga
       61 cgtggtcacc gtcccgcctg ccgttctcca gtcgctcgtc aaacacccgc tgacggacat
      121 tggtcttcgc caattcgttg ctgactggga gaaaacgggg cagaaaatca tctagctatc
      181 ggagtccaag gtgtccggat ttgcgccagc ttccggagac cgaagaagtc aagaagcttt
      241 gccgcacgac ttcccgtctt cgcgacgaga cgtgcttcga ggtaaggata tgggggattt
      301 ccagccactc agaggtattc gcgtcgtcga aatgagccac atgatcatgg gtccttcatg
      361 tggcatgttc cttggctttc ttggcgctga ggtcatcaag atcgagccgc cccagggcga
      421 caagacccgc tatctctctg gtatgggaag cccattcttt ccgctcttca atcgtggcaa
      481 gaaatcagtg cagctcgata cgcggaccga agcaggccgg gaggcgctgc acaagctgct
      541 cgcgactgca gacgttttcg tcgagaactt ccgggacgat tcgctggcca agatgggcgc
      601 cgacctcgca gagctgcggg ctcgctatcc caagctcatc atcgtctcgc acaaaggatt
      661 tctgacgggc ccctatcagc accgcaccgc gctcgacgag gtcgtgcaga tgatgaccgg
      721 cctcgcctac atgaccggcc cgagcgggcg gccgctgcgt gtcggctcgt ccgcgaacga
      781 catcatggga gggctcttcg gggcgctctc agtcgtctcg gcgctgctcg agcggcagtc
      841 cacgaacaag ggccgaaata tccgcgtcgg cctgtttgaa aactgcctcc tcctggtcgc
      901 acagcacatg gtgcagtacg agctggaggg cgttaacccg ccacccatgc cggagcgcga
      961 cttctcctgg ccagtctacg acatcttcac gaccgcagac ggcaagcaac tattcgttgg
     1021 cgccgttacg gaggggcagt gggcgaccct ttgcgatatc ctcggcctaa cggagctgaa
     1081 gtctgatccg cgattgaaga cgaagatgga catgatcaac gcgcgctcct ggaccttgcc
     1141 aattgtggag cgtgcgatcg caggacgtca gtctgccgat ctcatcagtg cctttgagga
     1201 agcgggaata cccttcgcgc ccattgccaa gccgtcggac atgttccaag acccgcacgt
     1261 catgcggcct ggtggccttg cgacgtcgcg catgcccgat gggaaaacat tccgcgctcc
     1321 ttcgctcccg ttcgaggtcg acgggcaaat gatgaccggt ggcggcgatg ttgcgagtgt
     1381 ggggcagcat acggaggaaa ttttggggca acttggcttg gatgccgaaa tgatcgcggc
     1441 ggctcgcggt gcggcatcgg ggaaatcggc aaaggcatag ttgccgttaa gaacagccag
     1501 caagaggcac tcgctcacgg ggagaacgcg taatgtctaa gttcaagagt ttggtagctg
     1561 ctatcgcgat tgcgatggta ggcgtaccgg gcgtcgttga ggccgcaacg aagatgcgct
     1621 gctcgcatca gctaccgccc gcgcaccacg tcgcgcaggt gatcgagcgt tgggcggagg
     1681 aggtgcgcac ggcttccgag ggcgagctgg atgtgcaggt gttcggctcg gacagcctcg
     1741 tcaaggccaa tcagaacatc accgccgttg ccaagggcga tatcgagtgc gccttctccc
     1801 tgaatttcca gtggggccgg acgctgccga ttatgaacgt gacggtcgcg cctttcgcgt
     1861 tcggcgacct ggaaatctgg cgcaagtggc cggattcaga agcggcgaag ttcctcgagg
     1921 aaaagctgct tgaaaagggc gttcgcaata tcgcgtggct gttccagacc aatacatccg
     1981 tattcacgtc caaggggcgt tttctggtga agccagagga tttcaagggc gtgaaactgc
     2041 gtgggctgaa cgcggccttc gatgctgcac ttgctgcact tggagcgacg ccgacgtcgt
     2101 tgccgggctc cgaggtgtat caggcgctcg cgacgggtgt gctcgatgca ggtttgaccg
     2161 acgtagcagc cgccgtttcg agaaaatact atgaggttca ggaccacttc acagtcgtgc
     2221 cggtcatcag cgcttacctg cacggatacg tgaacccgaa gtggtacgat gggctatcgg
     2281 acaagtcgaa ggcagcgctg gaaagcgcag ggaagaaggc ggcgctttgg gccgtggagg
     2341 cttccgagaa ggctggcgct gcggctcctg atcagcttcg cgagaaaggc gtgaaggtcc
     2401 atatcgcgac gcccgaggag atcaaggcgc tcgaggcgat catgcggcca gctttcgacg
     2461 agaaattcgc gaccgatcct gatgcgcaga agctgatcga gctcatcggg aaaatttgag
     2521 atggaaggcc tggagcaagg ccaagacaat cagccaacag cggaggaggt tcagccctcc
     2581 tccttgctcg cacgtgtggc ttccgctctc gcctggctcg ggggagcact gagcgccttg
     2641 ctcgtgctca taatactagg acttgtcacc tacgcggtgg tgcaacggta tgtgtttgac
     2701 cgcccattgg tgtggtcaga ccccgttgca ggatatctgg ttgtcgcatt gatgctgggc
     2761 gccgccgagg ctttgcggcg aggtgaccac atctcaatcg acctgctgag taaccaactt
     2821 ggcgcgcgcg gccgccgagc tgtcgcttat ttctccgatc tggcagtgct cgcgttcgct
     2881 gtggtgcttg gctggagctg ttgggagcag atcagctttg cctacgattt cgggtcgttt
     2941 accgcagatc acctcgaaat tccgagctgg atcccgatgg cgccgctgct cgccgcctgc
     3001 gttctccttg cgctgatggc ggcagcccgc ctgctggatg cattagcgcg aggagcgcag
     3061 cgttgacgat cttcctcgtc tttgcagggc tgcttgcgct gttgctctcg ggcgttc
//
LOCUS       DASUMG010000072         2926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1134059, whole genome shotgun sequence.
ACCESSION   DASUMG010000072 DASUMG010000000
VERSION     DASUMG010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134059"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VFX46_01150"
     CDS             complement(<1..1050)
                     /locus_tag="VFX46_01150"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HEX5599222.1"
                     /translation="MRAIATAAVGALGLALLGTPGPATAAEKISLRIGAGHPAAATWI
                     TTIRELFMPRFAERVKAETEYEIEWTEAWGGSVCKLGECLEAVEAGLLDMADLQTPFE
                     PSKLMAQNFSYFVPFGSGDPILAAELNRRTYEEVPALKQMLEDRYNQVFVGVGILSNY
                     GLVTTFQWEKIEDLQGQKIAAAGPNIPWVQSVGVVPVQSNLNEAYTSFQTGVYNGWVM
                     FPDGVTSFRLEEVTKQFSITGFGAIATPLLTINKDTWDSLPPEVQKIMLEVGAEWNKR
                     AGEFTAERQKQALAKMKEAGLRIKELTFEEKKAWAAKLPNIPKERYEEMKKLGQPGEA
                     VYHYIKLLKEAGHEFP"
     gene            complement(1081..2013)
                     /locus_tag="VFX46_01155"
     CDS             complement(1081..2013)
                     /locus_tag="VFX46_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5599223.1"
                     /translation="MAKARKAIITCAVTGAIHTPSMSEHLPITPDEIVEQALEAAAAG
                     ASVLHLHARDPETGKPDQTPEAFARFLPRIKQQTNAVINLTTGGSPYMRVEERVKPAA
                     QFKPEVASLNMGSINFGLYHLLNRYKSFKFDWERQHLEATRDLVFRNSFQDIEYILKT
                     CYGNDTRFEFECYDIAHLYNVKHFLDRGLVKPPLFIQSVFGILGGIGTHPEDVIHMKR
                     TADRLFGDLYQWSVLGAGAAQMRIAAMAAAMGAHVRVGLEDSLWAGPGRLAKSNAEQV
                     RMVRQILEGMGIEVATPDETREILALKGGDKVEF"
     gene            2264..2860
                     /locus_tag="VFX46_01160"
     CDS             2264..2860
                     /locus_tag="VFX46_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5599224.1"
                     /translation="MSPEERERMILKGAMQFFAERGFAADTRELARGLGVSQSLIYKY
                     FSNKRELLDRVYEETFVSRWKEDWEPFLRDRSIPFEERLRKFYLDYASVVNDKIWIRI
                     ALRSSLAGHRLTRRYIVNYIDRLLHVIARELRHEQGLRTSGPLSALELELAWHLHSTL
                     IYYLIRREVHGVPVCKDIAAVVNTIVTNFLYGIQKKRT"
BASE COUNT          605 a          874 c          778 g          669 t
ORIGIN      
        1 cgggaattca tgaccggctt cttttaggag ctttatgtag tgataaacgg cttccccggg
       61 ctggcccagc ttcttcattt cttcgtaacg ctctttcgga atgttgggaa gcttcgcagc
      121 ccaagccttc ttttcttcga aggtgagctc cttgatccgg aggcccgcct ctttcatttt
      181 ggctagcgcc tgcttctgcc gttcggccgt gaattcgcca gcgcgcttgt tccactcggc
      241 gccgacctcc aacatgatct tctgtacttc gggcggtagg ctgtcccacg tgtctttgtt
      301 gatcgtcagc agcggcgttg cgatggcacc gaatccagtg atggaaaact gcttggtcac
      361 ctcctccagg cggaatgacg tcacgccatc tggaaacatc acccagccgt tataaacgcc
      421 cgtctggaac gacgtatagg cttcattcag gttcgattgc accggcacga cgccgacaga
      481 ctgaacccaa gggatattcg gtcctgcagc agcgattttc tgcccctgaa ggtcctcgat
      541 cttctcccac tggaacgtcg tgaccaaccc gtagttgctt aggatgccca ctcccacgaa
      601 aacctgattg tagcggtctt cgagcatctg cttgagagcc ggcacttctt cgtaggtacg
      661 gcgattgagc tcggccgcca gaatcggatc cccagaaccg aagggcacaa aatacgaaaa
      721 gttttgagcc atgagcttgg acggctcgaa tggcgtctgc aagtcagcca tgtcgagcag
      781 gccagcttcc actgcctcga gacattcacc cagcttacaa accgagccgc cccaggcttc
      841 cgtccactcg atttcgtatt cggtctcggc ctttacccgt tcggcaaagc gcggcatgaa
      901 aagctcacga atcgtcgtga tccacgttgc tgccgccgga tgtcccgcgc caattcgcaa
      961 cgagatcttt tcagcggccg tcgctggccc cggcgttccc aacaatgcca agcccagcgc
     1021 gccgacagcg gccgtagcga tcgctctcat atctgctcct ccctacttga cgcgctctat
     1081 ttaaaactcg accttatcgc cgcctttgag cgcgaggatt tcacgcgtct cgtcgggcgt
     1141 tgcgacctcg atgcccatac cctcaaggat ctgccgcacc atgcgaacct gctccgcgtt
     1201 ggacttcgcg agtctcccgg gtcccgccca aagcgagtcc tcgagcccga cgcgaacatg
     1261 agcccccatc gcagccgcca ttgcggcaat gcgcatttgc gcggcgccgg cacctagcac
     1321 agaccactga tacagatcgc cgaacagtct atcggccgtc cgcttcatgt gaatcacatc
     1381 ttccggatgc gtgccgatac cgccaaggat tccgaacacc gactgaatga acagaggcgg
     1441 cttcaccaag ccacgatcca ggaaatgctt gacattgtag agatgagcga tgtcgtagca
     1501 ctcgaattca aagcgcgtat cgtttccgta acaggtcttg aggatgtact cgatatcttg
     1561 gaagctgttg cggaaaacga gatcgcgcgt tgcttcgagg tgctgcctct cccaatcgaa
     1621 cttaaaactc ttatagcgat tcagaagatg atacaggccg aagtttatgg aacccatatt
     1681 gagggaggcg acctcgggct tgaattgagc ggccggtttg acgcgctctt ccaccctcat
     1741 atacgggcta ccgccggtcg tgagattgat aacggcattc gtctgctgct tgatgcgtgg
     1801 caagaaccgt gcgaacgcct cgggagtctg atcgggcttg ccagtctccg gatccctcgc
     1861 gtgcaaatgc agaacggatg cgccggccgc tgccgcctcc agggcctgct cgacgatttc
     1921 atcgggcgtt atggggagat gctccgacat ggacggagta tggatcgcgc ccgtaactgc
     1981 acacgtgatt atcgctttcc gtgcttttgc catcgcgttg ctcctaaccg ccttttcgat
     2041 gtccgctcga ccgccgacgt ccgcttgact gtgctagtga gcaattgttc actattttac
     2101 gaaggtgtca atcgtctcgc gcgggattat tttcatactc agccactgtg gaccaacggc
     2161 ggagagtgct ttgactgaac gaagcgacat ccccacaaag ttgcaggaag caagacctac
     2221 accggttgat ggatctgggc gcaagctcag gaagcgcatc cgcatgtcac ccgaggaacg
     2281 cgagcggatg atcctcaagg gcgcaatgca gttcttcgcc gaacgcggtt tcgccgctga
     2341 tacgcgcgag ctggcacgcg ggctcggtgt ctcgcaatcc ctgatctaca agtacttcag
     2401 caataagcgg gagctgttgg atcgggtcta tgaggaaacg ttcgtatcgc gctggaaaga
     2461 ggactgggag ccctttctcc gcgaccgatc cattcctttc gaggagcggc tgcgaaagtt
     2521 ctatttggac tacgcttccg tcgtcaacga caagatctgg attcggatcg ccttgagatc
     2581 cagcttggcc ggccatcgtc tgacgcggcg ctacatcgtc aactatatcg atcgactgtt
     2641 gcacgtgatt gctcgtgaac tgcgccacga gcaaggattg cgaacatcgg gccctctcag
     2701 cgctctagag ctcgagctcg cctggcatct ccattctacc ctgatctatt atttgatccg
     2761 ccgggaggtg cacggagtgc ccgtttgcaa agatattgcg gccgtggtga atacaattgt
     2821 gaccaatttt ctgtatggga tccaaaagaa gcgcacttaa ccggccgact acctctacaa
     2881 ttacctctcc cccgctgggg gagaggtcaa aaaagtatac agctta
//
LOCUS       DASUMG010000073         2367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_331098, whole genome shotgun sequence.
ACCESSION   DASUMG010000073 DASUMG010000000
VERSION     DASUMG010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331098"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1740
                     /locus_tag="VFX46_01165"
     CDS             238..1740
                     /locus_tag="VFX46_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX5599225.1"
                     /translation="MAKRVLIVDDDPAQRRILEECVKRFGFETKTAASGDQALHILTG
                     PEKDDIVLVLLDLVMPDVDGTAVLEQLRGRPGSPPVIVQTAHGSIDGAVNAIRAGAVD
                     FIVKPISPERLEVSINTALKLRALSGEVTRLRRKSEGTLTFDDLIAGKAMERVVSLGR
                     RAARSNIPVLIEGESGVGKELIARAIQGESDRAGKPFVTVNCGAIPENLVESILFGHE
                     RGSFTGAIEKRIGKFQEADGGTLFLDEVGELPLDAQVKLLRALQDGEIDPVGAKRPVK
                     VNIRLISATNRDMIELVKSGRFREDLFYRLNVFPIWVPPLRERLDDVPELAYHFLARF
                     AAEEGKNVTSISEQALQLLMRYSWPGNVRQLENAVFRAVVLADGPELTVAEFPQIAMH
                     VEGFAPEVPPAPPPKEPELYTGPAMIGADDRVPRTIELKQSDGARSIGIPAVTDTGEV
                     RSLVDVEADMIRLALGRYRGHMSEVARRLGIGRSTLYRKMQEYGLNPRPH"
     gene            1909..>2367
                     /locus_tag="VFX46_01170"
     CDS             1909..>2367
                     /locus_tag="VFX46_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599226.1"
                     /translation="MRPALTLLSGLTLSAVLTSAALATAEPERTEAGDVSRSAVSAPV
                     SGTTDAVSAEEAPEVAGPATAAIEPRDAVDDEAAGLSPESDSGEPATSESASESEDLE
                     AGSEPAGVALEPDTDDPAASVEAVADPGLDPSPEQEAASSEPQEASTPEAA"
BASE COUNT          466 a          680 c          763 g          458 t
ORIGIN      
        1 atcattcagg gactcccgtt ctaccttgtt ctattcgcct ggggcatgaa gcctaatgtg
       61 cgggccagcg gcgcggggtt caagcaatgc gcgggcaggt ccacagcccc gcattggttc
      121 cagaggcaac cgcttgttta cggatgccgt gctcttgtgc cggagatgga ctgagcatgt
      181 gagcatccgc atgccagttt cgattgtgtt gcgttgcgta ttgagttcga gttgatcatg
      241 gcaaagcgcg ttttgatcgt cgacgacgat ccggcccagc gccgtattct ggaagagtgc
      301 gtcaagaggt tcggtttcga aaccaagacc gctgcgtcag gggatcaggc cctccacatc
      361 ctgactggcc ccgagaagga cgacatcgtt ttggtcctcc tcgatcttgt aatgcctgac
      421 gtcgatggca cggctgtgct ggagcagctt cgcggaagac caggctcgcc gccggtaatc
      481 gtacagacgg cacacggctc catcgatggt gccgtcaatg ccatacgtgc gggcgcggtg
      541 gacttcatcg tcaagccgat cagccccgaa cgacttgagg tctcgataaa cactgcgctc
      601 aagctgcgcg cgttgagcgg cgaggtcacg aggctcagac gaaaaagcga agggacgctg
      661 accttcgacg atctcatcgc cggcaaggca atggaacgag tggtctcgct cggcaggcgt
      721 gcagcccgtt ccaatattcc agtgctcatc gaaggggaat cgggtgtcgg caaggagctg
      781 atcgcccgtg ccattcaggg cgaaagcgat cgggccggca agccgttcgt caccgtcaat
      841 tgcggtgcca tccccgagaa cctcgttgag agcatccttt tcggccacga gaggggatcg
      901 tttaccggcg cgattgaaaa gcgcatcggc aagttccagg aggctgatgg cggcacgctg
      961 ttcctcgatg aggtgggcga gctgccgctg gatgcccagg tgaagctgtt gcgtgctctt
     1021 caggatggcg agattgatcc cgtcggggcg aaacggccag tcaaagtcaa catccggctg
     1081 atctcggcga ccaaccgcga catgatcgag ctggtgaaat cgggccggtt ccgcgaggac
     1141 ttattctacc gtctcaacgt attcccaatc tgggttccgc cgctgcgtga gcggctcgat
     1201 gacgtgccgg aacttgctta ccatttcctg gcgaggttcg cggcagagga aggcaagaac
     1261 gtcacctcca tcagcgagca ggcattgcaa ttgctcatgc gctattcctg gcccggaaac
     1321 gtccgccagc tggagaatgc ggtattccgc gccgtcgttc tggctgacgg ccctgagctg
     1381 acggtcgccg agttcccaca gattgcgatg cacgtcgaag gctttgctcc agaggtgccg
     1441 cctgcgccgc cacctaagga gccggagctt tacactggac ctgccatgat cggcgccgat
     1501 gaccgggtgc cacgtacgat cgagctgaag cagtcggacg gcgccaggtc gatcggcata
     1561 ccggcggtta ccgatacagg tgaggttcgc tcgctggtcg atgtcgaggc ggacatgatc
     1621 cggctggccc tcggtcgcta tcgggggcac atgtccgaag ttgcacgccg gctgggaatc
     1681 ggtcgttcga cgctctaccg gaagatgcag gaatatgggc tgaatcctcg ccctcactga
     1741 ggtggagatc acgccttatt tacgtgtcgg aaggaggcat tgaacggagc gtttgtcctt
     1801 tcggccacag ttccgacaaa agaagattct caggaaacat caggcgtgta acagttgctg
     1861 gctgagctgg agcaatgtac ttaccgctca gccgcatgga ggaccctgat gcgacctgct
     1921 ttgactttgc tctccggact gacgctgtcc gccgttttga caagcgcagc actcgccacg
     1981 gcggagccgg agcgcaccga agcaggtgat gtcagcaggt cagccgtatc ggcacctgtc
     2041 agcggtacga ccgacgcagt atctgctgaa gaggcgcccg aagttgctgg accggcgaca
     2101 gctgcgatcg agccgcgcga cgccgtcgac gatgaggctg cgggcctgtc gcctgagtct
     2161 gactcgggcg agccagcaac gagcgaaagt gcgagcgaat ctgaggatct cgaggccgga
     2221 tcagaaccgg ccggcgtggc gctcgagcca gacactgacg atccggctgc gtcggtggaa
     2281 gcagtagccg atcctggtct cgacccgtcg ccggagcagg aagcagcgag ttcagagcct
     2341 caagaggcgt cgacgccgga agcggca
//
LOCUS       DASUMG010000074         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_732609, whole genome shotgun sequence.
ACCESSION   DASUMG010000074 DASUMG010000000
VERSION     DASUMG010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732609"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VFX46_01175"
     CDS             <1..1293
                     /locus_tag="VFX46_01175"
                     /inference="COORDINATES: protein motif:HMM:NF006052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5599227.1"
                     /translation="FVKWAAVIRSIDRIPEYVSRAFHVASSGRPGPVVLGLPEDMLSS
                     KAEVRDARAARVTEPAPSAATMAELADLLATAQRPLMIVGGPGWSPEVQSRIERFAER
                     FDLPVAAAFRFQDYMDNRHPSYVGHAGIGIDAQLAAAIRDADLLIVVGARLGEMTTSG
                     YTLVDIPNPAQRLVHVHPDPNELGTVYAPDLPITATAAQFSAALDSLKPPAQIPWKQR
                     RADLRAAYEATLKPIPTPGAVQLEQVVRTVSEMLPQDGIVASGAGNYTAFVHRYFQYK
                     GYRTQLAPTSGSMGYGLPAAIAAKLAYPTRPVVAFAGDGCFMMTSQELATAVQYGLNI
                     VTIICNNGVYGTIRMHQEKKYPGRIVGTTLVNPDFAAFARSFGAHGETVSKTEEFRPA
                     FERALAAGKPSVIDLKIDPEAITPRQSLSEIRASSSKG"
     gene            complement(1578..>2496)
                     /locus_tag="VFX46_01180"
     CDS             complement(1578..>2496)
                     /locus_tag="VFX46_01180"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5599228.1"
                     /translation="QRNFSGVIAPVLTPFGEDGAPDPDRFIEHAEWLLEDGCTALAPF
                     GTTSEANSLGLDERMELLEELVDSGIDPAKLMPGTGSCSVTDATILTQHAVDLGCGGV
                     LMLPPFYYKNPSEEGLFRFFAEVIDDVGDDRLRVYLYHIPPVAQVGFSLSLIDRLRKE
                     FPGIVVGLKDSSGDWSNMKAILDAFPDFELFPGSELFLLEGLRNGAAGVISATANVSA
                     RAMRRLYDDWRGPNAEALQEEISALRRTIQSFPVIPTLKALIAHYRNDRGWAEVRPPF
                     TPLPPAEAERAIKLLADQHNFRLEFAEAA"
BASE COUNT          455 a          857 c          750 g          434 t
ORIGIN      
        1 ttcgtgaagt gggcggccgt catccggtcg atagatcgca ttcccgagta cgtgagccgt
       61 gccttccacg tcgcttcctc cggccggccc gggccggtgg tcctcggatt acccgaggac
      121 atgctctctt ccaaggcgga agtgagggac gcccgcgcgg cccgcgtcac agagccggca
      181 ccgagcgcgg caacgatggc cgagctggcc gatctgctcg ccacggccca gaggccgctg
      241 atgatcgtcg gcggccccgg atggagtccc gaggtgcagt cgcggatcga gcggttcgct
      301 gaacgcttcg atctgcccgt tgcggccgcg tttcgcttcc aggactacat ggataaccgc
      361 cacccctcct acgttgggca tgccggcatc ggcattgatg cccaactggc cgcagctatc
      421 cgcgatgccg acctgctgat cgtcgtcggc gcccgtctcg gcgagatgac caccagcggc
      481 tacacgctcg tcgatattcc caacccggcg cagcggctcg ttcacgtcca tcccgatccc
      541 aatgaattgg gcacggtcta cgccccagac ctcccgatca cggcaacggc ggcgcagttc
      601 tcggcggcgc tcgactcgct gaaaccgcct gcgcagatcc cgtggaagca gcgccgcgcc
      661 gatcttaggg ctgcctacga agcgacgctg aagccgatcc ccacgccggg cgccgtgcag
      721 ctcgaacagg tcgtccgcac ggtttccgaa atgcttccgc aagacggcat cgtcgccagc
      781 ggcgccggca actacacggc attcgtgcac cgctatttcc aatacaaggg gtaccgtacc
      841 cagctcgcgc ctacttccgg ctctatgggc tacggactac ctgccgccat cgccgccaag
      901 ctcgcctacc cgacacggcc ggtggtcgcg ttcgccggcg acggctgctt catgatgacg
      961 agccaggagc tggccacagc cgtccagtac ggtctcaaca tcgtgaccat catctgcaac
     1021 aacggcgtct acggcacgat ccgcatgcat caggagaaga agtatccagg gcggatcgtc
     1081 ggcacgacgc tggtcaatcc cgatttcgcc gcgtttgccc gcagcttcgg ggctcatggc
     1141 gagacggtga gcaagacgga ggagttccgg cccgcgttcg aacgcgcgct ggctgctggg
     1201 aaaccctctg taatcgacct caagatcgat ccagaagcga tcacacctcg gcagtcgctg
     1261 tccgagatcc gcgcgtcgag tagcaaggga tgatccgcag tacacctggt tcgcggtaag
     1321 ttcgcgttaa acgtgttctg accatttgct cgaccactta tgcgcgaaac gctacaagac
     1381 cattgcgacc tcgcgctgag ccgatcggat ctgcacctcc ctgaccgaac cggcaagcgt
     1441 tgagacgctc ctcccagcgc tcaggtggca gcgccagggc cgagcagaag cgcaacgact
     1501 tccttgatga ctctcccccc tccaacctcc ccctcctcga cagaggaggg ggccttcttg
     1561 ttctgctgcc gttggggtca ggcagcttca gcgaattcga gccggaagtt gtgctggtcc
     1621 gcgagcagtt tgatggcccg ctcggcttcc gccggtggaa gcggcgtgaa cggcggccgc
     1681 acctctgccc agccccggtc gttgcgataa tgcgcaatca gcgccttcag cgtcgggatg
     1741 accgggaagc tctggatggt cctccgcagc gccgagattt cctcctgcag ggcctccgca
     1801 ttcggcccgc gccagtcgtc gtagagccgc ctcatcgcgc gcgcgctcac gttggccgtg
     1861 gcggaaatca ctccagccgc tccgttgcgc agcccctcga gcaggaagag ctccgaaccc
     1921 gggaacaact cgaaatccgg gaaggcgtcg aggatggcct tcatgttgga ccagtcgcct
     1981 gaggaatcct tcaggccgac cacgatgccc gggaactctt tcctcaggcg atcgatgagc
     2041 gacagcgaga aaccgacctg ggcaaccggc gggatgtgat aaagataaac ccgcaagcgg
     2101 tcatcgccga catcgtcgat cacctcggcg aagaaccgga aaagcccctc ctcgctcgga
     2161 ttcttgtagt agaacggcgg cagcatcaac acgccgccgc agccgagatc caccgcgtgc
     2221 tgcgtaagaa tggtcgcgtc ggtcacggag catgagcccg ttccgggcat cagcttggct
     2281 ggatcgatcc cgctgtcgac cagctcctcc agcagctcca tccgctcatc gaggccgagc
     2341 gagttcgcct cgctcgtcgt cccaaacggt gccagcgccg tgcagccatc ttcaagcagc
     2401 cattctgcat gctcgatgaa gcggtccgga tccggcgcgc catcctcgcc gaatggcgtc
     2461 agaaccggcg cgatcacgcc ggagaaattg cgttgg
//
LOCUS       DASUMG010000075         3824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_170568, whole genome shotgun sequence.
ACCESSION   DASUMG010000075 DASUMG010000000
VERSION     DASUMG010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170568"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFX46_01185"
     CDS             complement(<1..782)
                     /locus_tag="VFX46_01185"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5599229.1"
                     /translation="MDGAGKRAIIVGGSMGGLVSGLLLLKAGWEVDVYERVAVELTAR
                     GAGIVTHPELDDVLVQAGVNPESAPAVTILERRTLDRKGRVIGKHVRRQTAISWNGLF
                     RILRAAFPEDRYHLGTEFLRCEETSSGVTAWFADGSVKTADLLIGADGIRSSVRRQFL
                     PECNPVYAGYVAWRGLIDESAFPAELHRELFEYFTFDLPPGEQMLGYPVSGRGDDNRP
                     GHRRYNFVWYRPADTDTLAWMLTDDSGHRHELSIPPPLISKAV"
     gene            complement(826..1929)
                     /locus_tag="VFX46_01190"
     CDS             complement(826..1929)
                     /locus_tag="VFX46_01190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5599230.1"
                     /translation="MEVSFFTMPVHPLNKPVAQTLKEDREAFLLADELGFAEAYCGEH
                     TTDLAENIPSCIAFLASLAYQTKRIRLGTGTVNLPNTHPAAVASQVAMLDHMLEGRLN
                     FGISPGGLLSDAEVFGNLDRDRNAMFVEAINMVLAIWAGEAPYDLKGQFWEISTRRTL
                     MPEIGQGAIIKPYQKPHPPIVGTVVAPYSKGVTAMAARGWEPISANFLLSKWVKTHWA
                     NYVEGCAQAGRPADPANWRVARSIFVADDAETARRYATDPNGPYVFYYRQLLTKMRAN
                     GRLNLFKEDQSMPDEAVTLEKVCDQLIFYGTPDQVADKILAFREEIGDFGHIVYVGHD
                     WADPALARRSMVLMAEKVMPAVNSAISSGGSAR"
     gene            complement(2048..2968)
                     /locus_tag="VFX46_01195"
     CDS             complement(2048..2968)
                     /locus_tag="VFX46_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5599231.1"
                     /translation="MLNNLKLQQIRYFVAVYEEGSFTAAARREHATQSGLSMQIKDLE
                     MTLGVRLFDRLPSGVTPTVVGRRFYEHATRILRDLGHLADDMATLSGAITGDVHVGLM
                     PTFTRAILAPALLSFSKAYPHVNVRVTEAYSGVLTQEVAKQALDFAIVPPGHAGTTIR
                     SRRLGTDREYLVTRADTDRPHLSPIDLSREKKPLRLIVPGPSNTRRGRIDAYLESCGV
                     KVEAILEMDAMMGTLDLVARSDWVTILSGVLCAPDRDGKARKLHPLINPELHVDYMLI
                     EPSTRSMSPAARLFAEAITVEMQRLIDEAA"
     gene            3078..3605
                     /locus_tag="VFX46_01200"
     CDS             3078..3605
                     /locus_tag="VFX46_01200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX5599232.1"
                     /translation="MNSAATTHPRAEARLRSFGSRELRDVLGCFATGVAVVTTIGDGG
                     APVGVTINSFNSVSLNPPLVLWSLSLTAPSLSAFRNHRAFAVNILSEDQVHIGRQFAR
                     PAPNKFAGIEWQPGYKGVPLIAGTAARLECRTWQRYEGGDHEIFIGEVIDMAATDKAP
                     LIFHRGRFARVDELR"
     gene            3638..>3824
                     /locus_tag="VFX46_01205"
     CDS             3638..>3824
                     /locus_tag="VFX46_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599233.1"
                     /translation="MSKVIAAIAGFGWWGKHMASRLRGNPHVAVKIVVEPVTERHDDI
                     RALGITPARELSEALNDP"
BASE COUNT          770 a         1175 c         1144 g          735 t
ORIGIN      
        1 accgctttcg atatcaacgg cgggggaatc gaaagctcgt ggcgatggcc gctgtcgtcc
       61 gtcagcatcc atgccagggt gtccgtgtcg gcaggacgat accagacgaa attgtaccgc
      121 cggtgtcctg gcctgttgtc gtcaccgcgg cctgaaacag ggtagccgag catttgctcg
      181 cccggcggaa gatcaaaagt gaaatactcg aacagctcac gatgcaattc ggcagggaaa
      241 gccgactcgt cgatgagccc ccgccatgca acgtagcctg cgtaaaccgg attgcactcc
      301 ggcaggaact ggcgccgcac gctggagcga atgccatcgg caccgatgag aaggtccgcc
      361 gtcttcacgg atccgtcagc gaaccacgcc gttacgccgg agctggtctc ctcgcagcgc
      421 aggaactcgg tgccgagatg atagcggtct tcggggaacg cggcccggag tattcggaac
      481 aggccattcc acgatatcgc cgtctggcgg cgtacgtgct tcccgataac tctgcccttt
      541 cgatcgagcg tccgccgctc caagatggta acggcgggcg cgctctcggg attcacgccc
      601 gcttgcacca acacatcatc gagctccggg tgggtgacga tgccagcgcc gcgggccgtc
      661 agctcgacag ccacgcgctc atagacgtca acttcccaac cggcctttaa aagaagcagc
      721 cccgatacga ggccgcccat cgacccgcca acgataatgg cccgttttcc agctccatcc
      781 atttccaagg cgtcgttctg attttcaatc tcgataaatc catgattatc gagctgaccc
      841 accggacgaa atggccgaat ttaccgcagg catcaccttt tcagccatca gcaccatgga
      901 gcgccgtgcc agagcgggat cagcccagtc gtggccgaca tagacgatgt ggccgaaatc
      961 tccgatctcc tcgcggaaag ccaagatctt gtcagcaacc tgatcgggcg tgccgtagaa
     1021 aatcagctga tcacagactt tttccagcgt tacagcctcg tccggcatcg actgatcttc
     1081 cttgaagaga ttcagtcgtc cattggcccg catttttgtc agcagctggc gatagtagaa
     1141 cacgtaaggc ccattcgggt cggtcgcata acggcgagcc gtttccgcgt cgtccgccac
     1201 gaaaatggag cgggccaccc gccagttggc cggatcagct ggacggccgg cctgggcaca
     1261 gccttccacg taattcgccc agtgcgtctt gacccatttg ctcaacaaga agttcgccga
     1321 aatcggctcc cagccacggg cagccatagc cgtcacgccc ttcgaataag gagcgacaac
     1381 ggttcctacg atgggcggat gcggtttctg gtagggcttg atgatagccc cttgcccgat
     1441 ctccggcatc aacgtgcggc gggtggaaat ctcccaaaac tgacctttga gatcgtaggg
     1501 tgcctcgccc gcccagatgg cgagcaccat gttgatcgcc tcgacgaaca tggcgttgcg
     1561 atcgcgatcc agattgccga acacttcggc gtccgacagc aatccgcctg ggcttatgcc
     1621 gaagttgagc cgcccttcga gcatgtgatc gagcattgcc acttggctcg ccacagccgc
     1681 gggatgggta ttcggcagat tcaccgttcc cgtgccgagc cggatgcgct ttgtctggta
     1741 ggccaggctg gcgagaaacg cgatgcacga tgggatgttc tcggccaggt cagttgtatg
     1801 ctcaccgcag taggcttccg caaagcccag ctcgtcagcc agcagaaagg cctcccgatc
     1861 ctccttcaag gtctgggcca caggcttgtt gagcgggtgg actggcatgg tgaagaagga
     1921 aacttccatg gatcagctcc gcacgcctcg taagaatgtc gatggatgtg cgaacgatac
     1981 ggcctccgag ccagaagaag gaatgatctt tcattatcct caccttcaaa tttcctgatg
     2041 ccccagatca ggctgcttcg tcgatcagcc tctgcatctc taccgtgatc gcctcggcga
     2101 agaggcgggc agcgggcgac atgctcctgg tgctgggctc gatgagcatg tagtcgacgt
     2161 gcaattcggg attgatcaag gggtgcagct ttctcgcctt tccgtcccga tccggcgcgc
     2221 acagcacgcc tgagaggatc gtcacccagt cggagcgggc cacaaggtcg agcgtgccca
     2281 tcatggcatc catctccagg atcgcctcca ctttgacgcc gcaactctcg agataagcgt
     2341 cgatccgacc acggcgcgtg ttcgaaggcc cagggacgat cagccgtagc ggtttctttt
     2401 cgcgcgacag gtcgatgggc gaaaggtgcg gccggtcggt gtcagcgcgg gtgaccagat
     2461 actctcgatc cgtcccgagg cgtcgcgatc ggatggtcgt gcctgcatgc cccggcggca
     2521 cgatggcgaa atccagcgcc tgcttcgcga cctcctgggt gaggacgccg ctgtaggctt
     2581 ccgtcacacg cacgttcacg tgcggatagg ccttcgagaa agacagaagg gcaggcgcga
     2641 gaatcgctcg cgtaaaggtt ggcatcagac ccacgtgcac atccccggtg atcgcgccgg
     2701 acagcgtggc catgtcgtcg gcgagatggc cgaggtcgcg caaaatgcgg gtggcgtgct
     2761 cgtaaaagcg ccggccaaca actgtcggcg tcacgcccga gggcagccgg tcgaacagtc
     2821 gcacgcccag cgtcatctcc aagtccttga tttgcatgga caaaccggat tgtgtggcgt
     2881 gctcgcgccg ggcagccgcc gtgaacgagc cctcctcgta gacggccaca aaatagcgga
     2941 tctgctgcag cttcaggttg ttcagcatgg tgcctatggg gatcagaact tctgatagcc
     3001 agcattaggc aatacaattt gagctgatag accatcgatt ttatgttcgc gcaatgacct
     3061 gcgcgaatgc tgaccaaatg aactctgccg ctacgaccca ccccagggcc gaagcgcgcc
     3121 ttcggtcttt cgggtcgcgc gagctgcgag acgttctggg ctgtttcgca acgggcgtgg
     3181 ctgtcgtcac cacgatcgga gacggagggg cccctgtggg cgtgacgatc aactcgttca
     3241 actccgtctc gctgaacccg ccgttggttc tatggagcct gtcgttgacg gcgcccagcc
     3301 tcagcgcttt ccggaaccac cgcgccttcg cggtcaacat tctttctgag gaccaggtcc
     3361 acatcggccg gcagttcgct cggcctgcac cgaacaagtt cgccggcatc gagtggcaac
     3421 cgggatacaa gggcgttccg cttattgcgg ggactgccgc acggctggaa tgcagaacgt
     3481 ggcagcgcta cgaaggcggg gatcacgaaa ttttcatcgg cgaagtgatc gacatggcgg
     3541 cgacggacaa agccccgctc atcttccatc ggggccgctt cgcccgcgtg gatgagcttc
     3601 gctgaatgaa cctagagcac ggcagaagag gagagatatg tccaaggtga tcgctgcgat
     3661 tgcgggtttc ggctggtggg gaaagcacat ggccagccgc ctgcgaggaa acccgcacgt
     3721 tgcggtcaag attgtcgtcg agcccgttac ggagcgccac gacgacatcc gcgctttggg
     3781 catcacacca gctcgcgagc tctccgaggc actcaacgat ccgg
//
LOCUS       DASUMG010000076         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_572144, whole genome shotgun sequence.
ACCESSION   DASUMG010000076 DASUMG010000000
VERSION     DASUMG010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572144"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="VFX46_01210"
     CDS             <1..1504
                     /locus_tag="VFX46_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599234.1"
                     /translation="KRGGELRFAVSAEPPDYDCHAATSFAFIHPVRPHYNTLLKFDPA
                     NYPKVVGDLAESWEQSADGLTYTFKLKQNVKFHDGTPMTSEDVKASYERIRNPQGGAR
                     SIRQAVYEDIESIETPDEYTVVFKLKKPNASMLTNFASPWDCIYSAAKLKEDPKFPER
                     NILGTGPFKFVEHAAGSHWVGERFEDYHVEGRPYLDRYRAIFITNTAARVNALQAGEV
                     LSEFRGHSPADRDKLVAALGDKVEVLESPWICSLVVTFNTEKKPFDDARVRRALSLAI
                     DRWQGAEALSKIALVRHVGGILRPGYELAATDKELEQFPGFGRDINAAREEAKKLLAE
                     AGVKDLSFKLMNRNVAMPYTPVGVYLIDQWRQIGVTVEHDQPETSAYISNLRSGNFEA
                     GLDFHCDAVDDPNLQLAKFVSANRSPINYSRANDPKLDELYEAQKGELDAEKRKQILR
                     EFERHALTEAYNIPVVWWHRIIVNWKQIKGWHMSPSHYLNQDLQDVWLDQ"
     gene            1589..>2016
                     /locus_tag="VFX46_01215"
     CDS             1589..>2016
                     /locus_tag="VFX46_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5599235.1"
                     /translation="MLRYTINRLLLMIPTLFGVAVLVFVLLRLMPGDIVELRYLMDGG
                     QISPEVLAQERARLGLDKPIWEQFVDWMTGIFVGDFGVSMWTGRPVWEEISSRFQLSF
                     QVAVMATILATLIAIPLGTLAALFKDTWVDRLIQVFAIAGL"
BASE COUNT          386 a          585 c          634 g          411 t
ORIGIN      
        1 caagaggggc ggcgagttgc gctttgctgt cagcgccgag ccgcctgact acgactgcca
       61 cgcggcgaca tcgtttgcgt tcatccatcc ggtgcgcccc cattacaaca cgctgctcaa
      121 gttcgatccg gccaattacc cgaaagtggt cggtgatctg gcagagtcat gggagcagtc
      181 ggcggacggc ctgacttaca cgttcaagct gaagcagaat gtgaagtttc acgacggtac
      241 tccgatgacg tcggaggacg tcaaggccag ctatgaacgt atccgcaatc ctcagggcgg
      301 cgcgcgctcg atccgccagg cggtctatga ggacatcgag tccatcgaaa cgcctgacga
      361 gtatacggtc gtcttcaagc tgaagaagcc caacgcctcg atgctcacca acttcgcttc
      421 gccgtgggac tgcatctata gcgcggcgaa gctgaaggag gacccgaagt ttccagagcg
      481 gaatattctc ggcaccggac ccttcaagtt cgtcgagcat gctgcgggct cgcactgggt
      541 tggcgagcgg ttcgaggatt atcacgtcga agggcgcccc tacctcgatc gctatcgtgc
      601 gattttcatc accaacacgg ctgcgcgcgt gaatgccttg caggcgggcg aggtcctctc
      661 agagttccgt ggccattcgc cggctgatcg tgacaagctc gttgcggcgc tgggcgacaa
      721 ggtcgaggtg ctcgagagcc cctggatctg cagcctcgtc gtgaccttca atacggagaa
      781 gaagccgttc gatgacgcgc gcgtgcgccg ggcgttgtcg ctcgccatcg accgttggca
      841 gggcgccgag gcgctgtcca agatcgcgct cgtgcggcat gtcggcggta tcctgaggcc
      901 tggctacgag cttgcggcga ccgacaagga gctggagcaa tttccgggct tcgggcgtga
      961 catcaacgcg gctcgcgaag aggctaagaa gctcttggcg gaagccggtg tgaaggatct
     1021 gtccttcaag ttgatgaacc ggaacgtggc aatgccttac acgccggtcg gcgtctatct
     1081 gatcgaccag tggcggcaga ttggcgtgac ggtcgagcac gaccagcccg agacctctgc
     1141 ctacatctcg aacctgcgga gcggcaactt cgaagctggg ctcgacttcc attgcgatgc
     1201 ggtcgacgat cccaacctgc agctcgcgaa attcgtctcg gcgaaccggt cgcccatcaa
     1261 ctattcgcgg gcgaacgacc ccaagctcga cgagctgtat gaagcgcaga aaggcgagct
     1321 ggacgccgaa aagcgcaagc agattctccg cgagttcgag cgccacgctc tgacggaggc
     1381 ttacaacatt ccggtcgtct ggtggcaccg gatcatcgtg aactggaagc agatcaaggg
     1441 ctggcacatg tcgcccagcc attatctgaa ccaggatctt caggacgtgt ggctcgacca
     1501 gtaacgagcc gaaatggggc ggccggcgtg gccgccccca aaatatcccg atcctgaatt
     1561 tgtagagagg gaaccgagcg gtcccgctat gctgcgttac accatcaacc gtctgctctt
     1621 gatgataccg acgctgttcg gcgtggcggt tctggtgttc gtgttgttgc gcttgatgcc
     1681 cggcgacatc gtcgagctgc gctacctcat ggatggggga caaatcagtc cggaggtgct
     1741 ggcgcaggag cgcgcacgcc ttggtctcga taagccgatc tgggagcagt tcgtcgattg
     1801 gatgacgggc atttttgtcg gcgatttcgg tgtctcgatg tggacggggc ggccggtctg
     1861 ggaagagatt tcgagccgtt tccagctttc gtttcaggtg gcggtcatgg ccaccattct
     1921 ggcgaccctc atcgccattc cgcttggcac gctcgccgct ctgttcaagg atacttgggt
     1981 cgatcgactt attcaggtct tcgctattgc gggcct
//
LOCUS       DASUMG010000077         3009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_10224, whole genome shotgun sequence.
ACCESSION   DASUMG010000077 DASUMG010000000
VERSION     DASUMG010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10224"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VFX46_01220"
     CDS             complement(<1..436)
                     /locus_tag="VFX46_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5599236.1"
                     /translation="MTRIPDFTTVAFDAQQSSVSSAAGSEAASVWNTPEGIVVKPAYA
                     AEDIAGLDFIDGLPGIPPYLRGPYPTMYVTQPWTIRQYAGFSTAEDSNAFYRRNLAAG
                     QMGLSIAFDLATHRGYDSDHPRVKGDVGMAGVAIDSIYDMRTL"
     gene            complement(517..2433)
                     /locus_tag="VFX46_01225"
     CDS             complement(517..2433)
                     /locus_tag="VFX46_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase subunit beta"
                     /protein_id="HEX5599237.1"
                     /translation="MSDETTELKLAANFEPATYEAWLALVAKVLKGSDFESRLVSRTL
                     DDIRIAPLYTRADTLPGTETARPGEAPYTRGFSRPGEKTGWDIRQFHSATDPAEANAA
                     IHEDLRGGVTSIALHIASPGTSGLPLNEGSLAAALDGVALEQTPIALVAGEAVVEAAN
                     ALESLWTERGIGDVARSGHFNADPLGTLAQIGQLSEPLNQSLARAVDLVRKTQSWPHV
                     TALVADGNPYHSAGASEAQELAAMLATLVAYLRACEVGGISPEQALPKIAVSLAVDDD
                     QFLSLAKLRAARRLIWRVAEACGAGDVARQVHLSAVSAWRMMAKRDPWTNIMRTTIAC
                     AAAAFGGAQSITLLPFTYALGKTDSFARRVTRNIQVVLQEESNLGRVADPAGGSWYVE
                     KLTDDLARKAWELFQGIESKGGMAAALTSGAFQDEIATVAERRNKAIATGRIEVTGVS
                     AFPLLGEDGVTAEPWPQAMPLTPDPAMKIKPLRQSRLAEPFEQLRDAADADAAAKGQR
                     ARVFVAALGTPADHGTRSTWVRNFLASGGIEALIGEGFTSSGDAGAAFANSGARVACI
                     ASSDAVYAELAEATAQALKSAGATHVFLAGRPGDQEAALKAAGVDAFWFAGQDRIAAL
                     QELHRLLEIAPSAA"
     gene            2694..>3009
                     /locus_tag="VFX46_01230"
     CDS             2694..>3009
                     /locus_tag="VFX46_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018070826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX5599238.1"
                     /translation="MEQDGKIFIGKSSKPEYLDLKLANRHGLITGATGTGKTVTLQVL
                     AEGFSDAGVPVFAADIKGDLSGIAAKGEPKDFILKRVEEVGLEGYENKAYPTIFWDVF
                     GEK"
BASE COUNT          569 a          923 c          995 g          522 t
ORIGIN      
        1 acagcgtgcg catatcgtag atggagtcga tggcgacgcc cgccataccg acatcgccct
       61 tgacgcgcgg gtggtcgctg tcatagcccc ggtgcgtggc caggtcgaag gcgatcgaga
      121 ggcccatctg gccggccgcg aggttgcggc ggtagaacgc gttcgagtcc tcggcggtgg
      181 agaagcccgc gtactgacgg atggtccacg gctgggtgac gtacatggtc gggtaggggc
      241 cgcgcagata aggcggaatg cccggcaagc cgtcaatgaa gtcgaggccg gcgatgtcct
      301 cggccgcgta ggctggtttg actacgatac cctcaggtgt gttccagaca gacgccgctt
      361 cggagcccgc agcagatgag acgctgcttt gctgggcatc aaaagcgaca gtggtgaaat
      421 cggggatgcg ggtcatgctc gtcaaaccag attggtacca gcgcaaatcg cggagaacgt
      481 gatctcagcc tcgatcgaac agtaacacat atcgcgtcac gcggccgatg gcgcaatctc
      541 gagcaggcga tgcaactctt gaagcgccgc aatccggtcc tggccagcga accagaaggc
      601 atccacgcca gcggctttga gagcggcttc ctgatctccg ggtcggccgg cgaggaaaac
      661 gtgggtggcg cctgcggact tgagcgcttg cgcagttgct tcggccagtt ccgcgtagac
      721 ggcgtccgag gaggcgatgc aggctacgcg cgcgccactg ttggcgaacg ccgcacccgc
      781 atcgccggag ctggtgaagc cttcgccgat gagcgcttcg atgccgcccg aggccaggaa
      841 gttccgcacc cacgtagaac gcgtcccatg gtccgccggg gtgccgagcg cggcaacgaa
      901 cacgcgagcg cgctggcctt tcgcggccgc gtcggcatcc gcagcatcgc gcagttgctc
      961 gaacggttcg gcaaggcgag attgtcgaag cggtttgatc ttcatggccg gatcaggcgt
     1021 gagtggcatg gcttgcggcc agggttccgc tgtgacgcca tcttccccca gcagcgggaa
     1081 ggctgaaacg ccggtcacct cgatgcggcc ggtagcgatg gccttgttgc ggcgctcggc
     1141 gacggtggcg atctcatcct gaaaagcgcc ggaagtgagc gcagcggcca tgccgccctt
     1201 cgattcgatg ccctggaaca gctcccacgc tttgcgggcc aagtcatccg tcagcttttc
     1261 gacgtaccag ctcccgcccg caggatccgc gacccggcca aggttggatt cctcctgcag
     1321 cacgacctga atgttgcgcg tcacgcggcg ggcgaaagag tcggtcttgc ccagtgcgta
     1381 ggtgaacggc agcagggtga tcgactgagc accaccgaag gcagcagccg cgcaggcgat
     1441 ggtggtgcgc atgatgttcg tccacggatc acgctttgcc atcatgcgcc aggcgctaac
     1501 ggctgacagg tggacctgcc gcgcgacgtc accggcgcca cacgcttccg ccacacgcca
     1561 gatgaggcgc cgcgccgccc ggagctttgc caaagacagg aactgatcgt cgtcgaccgc
     1621 gagcgacacg gcgatcttgg gcagcgcctg ttcaggggaa atgccgccca cctcgcaagc
     1681 ccgtagatag gccacgaggg tggcgagcat cgccgcaagc tcttgcgcct cgctggcgcc
     1741 agcggagtga tagggattgc cgtcggctac gagcgcggtc acgtggggcc aagactgcgt
     1801 cttgcggacc aggtcgactg cgcgggcgag gctctgatta agcggctcgc tgagttgtcc
     1861 aatctgggcc aacgttccga gcggatcggc gttgaagtga ccggaccgcg cgacatcgcc
     1921 gataccgcgc tccgtccaga gggattccag cgcatttgcc gcttcgacca cagcttcccc
     1981 ggctacgaga gcgattggcg tctgctcaag cgccactccg tccaacgccg cagccaacga
     2041 cccctcattg agcggcaacc ctgatgttcc cggcgatgcg atatgcaggg cgatggacgt
     2101 aacgccaccg cgcaaatctt catggattgc agcgttggct tctgccggat cagtcgcgga
     2161 atggaattgc cggatgtccc agccggtctt ctcgccgggg cggctgaagc cgcgcgtgta
     2221 tggcgcctca ccgggtcggg ccgtttcggt gccgggcaac gtgtcggcgc gcgtgtaaag
     2281 cggcgcgatg cgaatgtcgt ccagcgtgcg ggaaacgagc cgcgactcga aatcgcttcc
     2341 cttcagaact ttggcgacga gcgcaagcca agcttcgtag gttgcaggtt cgaagttggc
     2401 tgcaagcttc aattccgtcg tttcgtcgct catccagcta caaccctctt ccatcgcggg
     2461 cctcccctcg agccggcccc gcccgcgggc acattactcg aactcggccg caatgccacg
     2521 gtcagataac atgccaggcg aagctgcgat attcagtgat taatcttgga tttgaaggcg
     2581 cggtcctttt cagtccaccg gtcgcatgtc atcacggtaa taatcgaggg tgcacacgaa
     2641 tcggtacggc gtgagttcta ctaccgggcg gcgcaagaca cggggagcca gcgatggaac
     2701 aggacggcaa gatcttcatc ggcaagagca gcaagccgga atacctggac ctaaagcttg
     2761 ccaatcgtca tggcctcatc acgggcgcaa cgggcaccgg caagaccgtg acgctgcaag
     2821 tgctggccga aggcttttcc gacgccggcg tgcccgtgtt cgccgccgac attaaggggg
     2881 atctttcggg gatagcagcg aagggggaac cgaaagattt catcctgaag cgggtcgagg
     2941 aggttgggct cgagggctac gagaacaagg cgtacccgac gatcttctgg gacgtgttcg
     3001 gcgaaaaag
//
LOCUS       DASUMG010000078         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_813690, whole genome shotgun sequence.
ACCESSION   DASUMG010000078 DASUMG010000000
VERSION     DASUMG010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813690"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VFX46_01235"
     CDS             complement(<1..367)
                     /locus_tag="VFX46_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX5599239.1"
                     /translation="MIIAIDGPAASGKGTLGKLIAEHFGFAHLDTGLLYRAVARDVLA
                     RGASIDDEQAAVECARSLDPETLSDPVLRQAGIGEAASIVARIELVREALLDFQREFA
                     RRKPGAVLDGRDIGTVVCPD"
     gene            complement(491..1816)
                     /gene="aroA"
                     /locus_tag="VFX46_01240"
     CDS             complement(491..1816)
                     /gene="aroA"
                     /locus_tag="VFX46_01240"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX5599240.1"
                     /translation="MTAPLSRPLTASSAAPLRGACRVPGDKSISHRALIFGALATGRT
                     RVSNLLESEDVLNTAKAVTALGAPARKSGDVWEILGRGVGGLSQPSEPLDFGNSGTGA
                     RLMMGVIAGHPITVRLIGDPSLSRRPMGRVLRPLQQMGLEVLENKETLPLTIRGTSDL
                     VPIEYELPVPSAQVKSAVLLAGLAARGETTVIEKEPTRDHTERMLRYFGAEIRSVAEG
                     GRTRITVKGNAELDGRDVVVPGDPSSAAFIAAAALIVPGSEVTIEGVLINPTRTGFYL
                     TLQEMGGDVTFLNMREEGGEPVADIRVRASSLTGVRVPAERAPSMIDEYPILAVVAAF
                     ARGRTEMLGLAELKVKESDRLTATAAGLIANGVEAVADGDTLIVEGTGRAPRGGGTVA
                     THLDHRIAMAFLTMGLASREPVTVDDTSVIATSFPEFRELMERLGARFA"
     gene            2013..>2392
                     /locus_tag="VFX46_01245"
     CDS             2013..>2392
                     /locus_tag="VFX46_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEX5599241.1"
                     /translation="MATKEARGTKRTCQNPECGSRFYDLNRDPIVCPICQTVYKLATA
                     PQIEEAQDEQAVRKPVKPELVADEEGLVGDLPLAEGDEDLADLEEADEALVGDEEEPF
                     IEPEDEDGGDVTDILGPVGEDDDE"
BASE COUNT          470 a          761 c          748 g          413 t
ORIGIN      
        1 catcggggca gacgaccgtg ccgatatccc gcccgtcgag cacggctccg ggcttgcgcc
       61 gcgcgaactc gcgctgaaaa tccaacagcg cttcgcgcac cagctcgatg cgagccacga
      121 tggacgcggc ctcgccgata cccgcctggc gcagaactgg atccgataac gtttcgggat
      181 cgagcgaccg ggcacactca actgcggcct gctcgtcatc gatcgacgct ccccgcgcca
      241 gcacgtcgcg ggccaccgcg cgataaagaa gccccgtgtc gaggtgagca aagccgaagt
      301 gctcggcgat aagtttgcca agcgtaccct tgcccgatgc tgccggtccg tcgatggcaa
      361 tgatcatgcc gttcccattc gtttggcatc cgttcgcacg cagcctgcag ccatgcgggg
      421 tcacctccac cgacctttgg aatgcctcat gattgtgcct tgcggaccgt taccacttgt
      481 ccgctgctcg tcacgcgaag cgtgcgccta gtctttccat cagctcccga aactcgggaa
      541 agctcgttgc gatcaccgac gtatcatcca ccgtgaccgg ctcccggctg gcaagcccca
      601 ttgtgagaaa agccatagcg atgcggtggt cgagatgcgt cgcgacagtt ccgccgcctc
      661 gcggagccct gcccgtgccc tcgacgatga gggtatctcc atcagcgacc gcctcgacac
      721 catttgcaat gagcccagcc gccgttgccg tcagccggtc gctttccttg accttaagct
      781 cagccagacc aagcatttcg gtgcggccgc gcgcgaaggc agcgaccacg gcgaggatcg
      841 ggtactcgtc gatcatgctc ggcgctcgtt ccgccggaac ccggactccc gtgagactcg
      901 acgcacgcac gcggatgtcg gcaacgggct cgccaccttc ctcccgcatg ttgaggaacg
      961 tgacgtcgcc acccatctcc tgcagcgtca gataaaatcc ggttcgggtg gggtttatca
     1021 ggacgccctc gatcgtcact tcggagccag gaacaataag tgctgccgct gcgataaagg
     1081 cggccgagct cggatcgccc ggcaccacga cgtcccggcc gtcgagctcg gcgttacctt
     1141 tgacggtgat acgagtacgc ccgccctcag caacggagcg aatctcggca ccaaagtagc
     1201 ggagcatccg ctccgtgtgg tctcgcgtcg gctccttctc aatcacggtc gtctcgcctc
     1261 tcgcggcgag ccctgcaagc aggacggcgc tcttgacctg ggccgagggc accggcagct
     1321 catactcgat tggcaccaga tcgctggtgc cgcgaatggt gagtggcagc gtctccttgt
     1381 tttcgagaac ctcgagcccc atctgctgca gcggccgcag aacccggccc atgggccggc
     1441 gggacaagga tggatcgcca atcagccgta ccgtgattgg atgtccggcg atgacaccca
     1501 tcatgagccg tgcgccggta ccagagttgc cgaagtcgag tggctcggaa ggttggctga
     1561 ggcccccgac accacggcct aggatttccc agacgtcgcc cgatttgcgg gctggagccc
     1621 caagggccgt cacagccttc gcggtgttga gcacgtcttc ggactcgagc agattgctca
     1681 ctcgcgtgcg gcccgtcgcc agggcgccaa aaatgagcgc gcgatgggaa attgacttgt
     1741 caccaggcac gcgacaagca ccacggagcg gggctgccga agaagcagta agcggtcggg
     1801 aaaggggagc tgtcatataa gaatatcggc cgtaaagagc ttcgattgcg ccggcgctca
     1861 gcccccggca ggctcgctgg cagatagaga ctggctgggc ctaagctgtc aaccgcggca
     1921 aggggacagg gggctgccaa ttacttttga catggtctgc cacccgtggc agacgcagct
     1981 gaatttcgcg atgaggacat tgaggaatta agatggcaac gaaggaagcg cgtgggacca
     2041 agcgcacctg ccagaaccct gaatgcggct cccgattcta tgacctgaac cgggatccaa
     2101 tcgtatgccc aatctgccaa acggtgtaca agctcgcgac tgctcctcag atcgaagagg
     2161 cgcaggacga gcaggcggtc cgcaagcccg tgaaacctga gttggtggca gacgaggaag
     2221 gcctggttgg tgatctcccg ctggccgaag gcgacgagga cctggccgac ctggaggagg
     2281 cagacgaagc gcttgtggga gacgaggagg agcctttcat tgaacccgag gacgaggacg
     2341 gcggtgatgt gaccgacatt ctcggtccgg ttggcgagga tgacgacgag ga
//
LOCUS       DASUMG010000079         2958 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_171198, whole genome shotgun sequence.
ACCESSION   DASUMG010000079 DASUMG010000000
VERSION     DASUMG010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171198"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFX46_01250"
     CDS             <1..935
                     /locus_tag="VFX46_01250"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HEX5599242.1"
                     /translation="LPDDPVCEPLLTNYFPEALRENFFEEIKAHRLRREIIVTGLTNA
                     MLNRGGPAMAVRLADESGRGPGDVAAAFLTVSSVFRLPDLWQAVDALDGKMPGQAQLD
                     LYLRIQDFLLEQVSSLLRMGPSDDLAATIAKHRAGVEELSHVFGECLTPAQLDRLASS
                     RQAMERSGATQEVAERIAALSVLSHAMTITRLARETNRPIVDAARVAFAASDYFHLDE
                     LKALAAGLQIRDYYDRLAVNGAIYTLETARRALSREVLHEAIGAADFAAWEREHGGRL
                     ARAKMALDEIAATGDLTVSRLTVAASQVRDLTSA"
     gene            967..2376
                     /locus_tag="VFX46_01255"
     CDS             967..2376
                     /locus_tag="VFX46_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599243.1"
                     /translation="MTPSRPAEAEPSTSPRAAAPMRALVSWALVDWALQPYYTLILTF
                     LFAPYFTSVVVGDPVRGQALWGYAAAIAGILIAVGSPVLGAMADEGGRRKPWVAVFAT
                     ILAAGMAALWWAMPGATDAALFLILVAFIAATIAAEFATVFTNAMMPTLVPSDRLGRL
                     SGIGWATGYVGGLLSLAVMAGLLVTNPATGRTLLGFEPLLSLDVVQREGDRMVGPFAA
                     AWAVVFLLPFFLFVPDRATGGLRSDHRSALAQLWQTLRELPRQRDVLYFLIARALYSD
                     GLTAIFVFGGIYGTAVFGWEAFERGIFGILLITIGILGAVIGGTLDDKFGAKRVVIGG
                     LVLLMIGCVGILSVTTGHVLFTIPIGEKMPGSQPFSTTGEIVFLIFAGLIAIAAAPNQ
                     AASRTLMARLSPPDRMTQYFGLFAFSGKVTAFLAPLTIAAVTSLTGSQRAGMAMILVF
                     LFAGLLILLPVRERQVSSE"
     gene            complement(2396..>2958)
                     /locus_tag="VFX46_01260"
     CDS             complement(2396..>2958)
                     /locus_tag="VFX46_01260"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5599244.1"
                     /translation="PWCSRASATSGTEAMSGAASSKGVQLRPATRADWPLIRKWLARP
                     DIQRWWGPQASTEAEVLMAMDSTHAICRIIEVDGEPVGYAHAVDASLWGEALPDDLVP
                     GTWDLDIFIAAESHRGKGIGETALRLLKDEVFATTLAVAVCIFASIENEAAVRAYEKA
                     GFRWRRVWHDPVTGPEWFMIAERPTR"
BASE COUNT          462 a          983 c          936 g          577 t
ORIGIN      
        1 cattaccgga cgatccggtc tgtgagccgc tgctgacgaa ctatttcccc gaagcactgc
       61 gggagaattt cttcgaggag atcaaggccc accgtttgcg ccgggagatc atcgtcactg
      121 gcctgaccaa tgcgatgctc aatcgcggcg gccctgctat ggccgtgagg cttgctgacg
      181 aaagcgggcg cgggccagga gacgtcgcag cggcattcct cactgtcagc tccgttttcc
      241 gcctgcctga tctctggcaa gcagtggatg cgctcgacgg caagatgccg ggacaggctc
      301 agctcgacct ttatctgcgg attcaggact tcctgctcga gcaggtttcc agcctgctgc
      361 gaatgggtcc gagcgacgat cttgcagcca cgatcgccaa gcaccgcgcg ggcgttgaag
      421 agttgagcca tgtcttcggc gagtgcctca cgccggctca gctcgatcga ctggcctcaa
      481 gccggcaggc gatggaacgc tccggagcga cgcaggaggt ggcagaacgg atcgccgccc
      541 tcagcgtcct ctctcacgcc atgaccatca cgcgactcgc ccgcgaaacg aaccgcccga
      601 tcgtggatgc ggctcgggtt gccttcgccg cgagcgacta cttccacctc gacgagctga
      661 aggccctcgc tgccggcttg caaatccgcg attactacga ccggcttgcg gtcaacggcg
      721 ccatttacac gctcgagacg gcacgccgtg ccctgtcccg tgaggtgctg cacgaggcaa
      781 tcggtgctgc cgattttgcc gcatgggaac gcgagcacgg cggacgactg gcgcgagcca
      841 agatggccct tgacgagatc gcggcgacgg gcgacttgac ggtctcacgg ctgacggttg
      901 ctgcatcgca ggtgcgcgat ctgacatccg cttgaagccg cgagccgccg caagggagac
      961 tggacgttga cgccatcacg gcccgccgag gccgagcctt cgacctcacc gcgggctgct
     1021 gctcccatgc gcgccctggt cagctgggcg ctggtcgact gggccctgca gccctactac
     1081 acgctcatcc tcacctttct cttcgcgcct tatttcacat cggttgtcgt gggcgatccg
     1141 gtgcgggggc aggcgctatg gggctacgcc gccgcgatcg ccggcattct catcgccgtg
     1201 ggtagcccgg tgctcggcgc aatggcagac gaaggcgggc ggcgcaaacc gtgggttgcc
     1261 gtcttcgcca ccatcctggc tgccgggatg gcagcgctct ggtgggccat gccgggggca
     1321 acggacgccg ctctcttcct gattctcgtt gcattcatcg cggcgaccat cgcggccgag
     1381 ttcgccaccg tgttcaccaa cgccatgatg ccgacgctgg tcccatcgga tcggcttggc
     1441 cggttgtcgg ggattggatg ggcgaccggc tacgtgggcg gactactcag ccttgccgtg
     1501 atggcagggc ttttggtcac caatcctgcg acgggacgaa cgctgctagg gttcgagccg
     1561 ctactgtccc tcgacgtcgt gcaacgagaa ggcgaccgga tggtcgggcc gttcgctgcg
     1621 gcctgggccg ttgtgttcct gctgcccttc ttccttttcg tgccggaccg ggcgactggg
     1681 ggcctccgct ctgaccaccg atcggccttg gcccagctct ggcagacgtt gcgggagctg
     1741 ccgcggcagc gcgacgtgct ctactttctc atcgcgcggg cgctctactc ggatgggctg
     1801 acagccattt tcgtgttcgg cggcatctat ggtaccgccg tattcggctg ggaggcattc
     1861 gaacgcggaa ttttcggcat actgctcatc actatcggca tcctcggtgc cgtgatcggg
     1921 gggacgctcg acgacaagtt cggcgccaag cgcgtcgtca tcggcggtct tgtgctgctg
     1981 atgatcggct gcgtcgggat tctctcggtg acgaccgggc atgtcctgtt cacgattcca
     2041 atcggggaaa aaatgccggg ctcgcagccg ttctccacca ccggcgaaat cgtgttcctc
     2101 atcttcgccg gtctgattgc cattgccgcc gcgcccaatc aggcggccag ccgaacgctg
     2161 atggctcggc tttcccctcc ggatcgcatg acgcagtact tcggcctgtt cgccttctcc
     2221 ggaaaggtga ccgccttcct cgcaccgctg acgatcgcgg ccgtcacgtc cctcacgggc
     2281 agccagcgcg ccggcatggc gatgattctc gttttcctct tcgccggcct cctcattctg
     2341 ctgcccgtac gcgaacggca ggtttcgtcc gagtagctcg atgtttctgc acacctcaac
     2401 gggtgggccg ttcagcgatc atgaaccact cggggcctgt gacgggatcg tgccagacgc
     2461 gccgccagcg aaagccggcc ttctcgtaag cgcgcaccgc cgcctcgttc tcgatcgaag
     2521 cgaatatgca gacggcaaca gcgagcgtcg tcgcgaacac ctcatctttg aggaggcgaa
     2581 gggctgtttc gccgatgcct ttgccgcgat ggctctcagc agcgatgaag atatccagat
     2641 cccacgtgcc gggaacgaga tcatccggca gtgcctcgcc ccaaagactt gcatcgacag
     2701 cgtgcgcata gccgaccggc tcgccatcga cctcgatgat ccggcagatg gcgtgcgtgc
     2761 tgtccatcgc catcagcacc tcggcttccg tgctcgcctg cggcccccac cagcgttgga
     2821 tgtcggggcg ggcgagccac ttgcggatga gcggccagtc ggcgcgcgtc gcgggcctga
     2881 gctgcacgcc tttcgaactg gctgccccgc tcatcgcctc agtgccggaa gtggcggacg
     2941 cccgtgaaca ccatggcg
//
LOCUS       DASUMG010000080         2415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_90967, whole genome shotgun sequence.
ACCESSION   DASUMG010000080 DASUMG010000000
VERSION     DASUMG010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90967"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VFX46_01265"
     CDS             <1..701
                     /locus_tag="VFX46_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HEX5599245.1"
                     /translation="PRIANFDDLDPLRLEPDVEVEMIRPGRPLPLDCDLIILPGSKST
                     ISDLEALRSEGWDIDIRAHVRRGGVVLGVCGGFQMLGQQITDPDGIEGPAGSASGLGL
                     LAVETVLSRNKTVQPVSGLDSLTGLPVSGYEIHLGVTSGPDTERPFVVIDGKADGARS
                     ADGRVLGTYVHGLFASDVFRAHFLSGFRSGEIHLLAYEARIDATLDALADHVARHIDC
                     DRLMAIAQSRIHKS"
     gene            complement(689..1669)
                     /gene="cbiB"
                     /locus_tag="VFX46_01270"
     CDS             complement(689..1669)
                     /gene="cbiB"
                     /locus_tag="VFX46_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HEX5599246.1"
                     /translation="MILEPFAPWLLLVALFADALIGDPPWLWRRIPHPVVVMGRLIGF
                     LDNNLNRKTWPFATRRVAGIIALLVVVSAGAGLGVVLQLLLSDSIPGKFAELLIVTVL
                     LAQRSLYEHVAGVKQAFDHGGLPAARMAVSRIVGRDPEALDEAGVCRAAIESTAENFS
                     DGIVAPAFWYLIAGLPGILAYKAVNTADSMIGHRSERYRAFGWAAARLDDLLNYVPAR
                     ISGFLLAAAAPAVGGSAESAVRAMLRDASKHKSINAGWPEAAMAGALGLALAGPRVYA
                     GGRVEDAWMNASGRPNAKPSDIASALLLLVATCLAHALLVAVVGLIAQLL"
     gene            complement(1666..>2415)
                     /locus_tag="VFX46_01275"
     CDS             complement(1666..>2415)
                     /locus_tag="VFX46_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599247.1"
                     /translation="INACADQLILALADREQILALTNYARDPDMSFYADRAKGLPTIR
                     GDAEEVLKLKPDLVLAGSFTRSATRAQLAAQGVRIATFSPARSIEDVKKHIREVASLL
                     GQEARGQALVAEIDAAFANLKPRSSRLLRALQLQRRAFAAGTDTLIHDIMQRLGIVNA
                     ATDLGIRYVGRTSLEAVLKAKPDVLILDSADPGAVDQGVALLSHPALEEVVPRHRRIV
                     IPSSQIVCASPGIALAARTLRDAAERILDEK"
BASE COUNT          479 a          720 c          734 g          482 t
ORIGIN      
        1 tgccccgcat tgccaatttt gacgatttag acccgttgcg tcttgagccg gatgtcgaag
       61 tcgagatgat ccggccaggt cgcccgctgc cgctcgattg cgatctcatc attttgcctg
      121 gctcgaaatc cacgatttca gaccttgaag ctctaagatc ggaaggatgg gacatcgata
      181 tccgcgccca cgtgcgacga ggcggtgtcg tgcttggcgt gtgcggcggg ttccagatgc
      241 tggggcaaca aatcaccgac ccggatggca tcgaaggccc ggcaggctct gcatcgggac
      301 ttggcctgct ggcggtggag acggtgctct ctcggaacaa aactgttcag ccggtttctg
      361 gtctcgactc gcttacgggc ttacctgtct ctgggtatga aattcacctg ggggtgacca
      421 gtggaccaga cacggaacgg cctttcgtgg ttatcgacgg taaggccgat ggtgcacgtt
      481 ctgcggatgg gcgagtgctc ggcacctatg ttcatggact ctttgcgagc gatgtttttc
      541 gcgcgcattt tctgtccggt ttccgaagcg gcgagattca cctgctggcc tacgaagcgc
      601 gaatcgatgc aacgctcgac gcactcgccg accatgtcgc gcggcatatc gattgtgatc
      661 ggctgatggc gattgcgcaa agccgtattc acaagagctg agcaatcaat ccgacgaccg
      721 cgactaggag agcgtgcgcc agacacgtag caacgagcag cagcagcgct gacgcaatat
      781 ctgacggttt cgcgtttggg cggccggagg cgttcatcca agcgtcttcg acacggccgc
      841 cggcataaac ccgtggcccc gccaaggcca acccgagtgc gccggccatg gctgcttccg
      901 gccaaccggc attgatggat ttgtgcttgc tcgcatcgcg gagcatggct ctgacggcgc
      961 tttctgcact gccgccaacg gctggtgcag cggcggcgag gaggaagcct gaaattcgcg
     1021 caggtacata gttgagcaga tcatccaacc gcgccgcagc ccagccgaat gcgcgatagc
     1081 gctcgctccg atgaccgatc atgctgtctg ccgtattcac ggccttgtag gcgagaatgc
     1141 ccggcaatcc tgcaatgagg taccagaacg ccggcgcgac aatcccatcg gaaaaattct
     1201 ccgctgtcga ttctattgcg gcgcggcaga cacccgcctc atccagggcc tcgggatcgc
     1261 ggccgacgat tctcgatacg gccattcgtg ccgccggtaa tccgccgtgg tcaaaggctt
     1321 gtttcacgcc ggcgacatgc tcgtaaagac ttcgctgagc gagcagaacc gtgacgatta
     1381 agagttctgc gaatttaccc ggaatgctgt cgctcaggag gagctgcaac accacgccca
     1441 ggccagctcc ggcactcacc acaacaagca gcgcgattat tccggccact cgtcgcgtgg
     1501 caaatggcca ggtttttcgg ttcaggttgt tatcgagaaa gccgatgagg cgccccatga
     1561 ccaccaccgg gtgagggatg cgccgccaca gccagggcgg atccccgatc aaggcatccg
     1621 cgaagagcgc aacaagcagc agccagggcg caaatggctc cagaatcatt tttcgtcgag
     1681 gatccgctct gccgcatcgc gcagggttct ggcggcaagc gcgatgcctg ggctggcgca
     1741 gacgatctga cttgaaggga tcacgatccg gcggtgccgc ggcaccactt cttccaatgc
     1801 aggatgacta agcagcgcga ctccctggtc gactgcacca ggatcggcgc tatcgaggat
     1861 gagaacatcg ggtttcgcct tgagaaccgc ctcgagcgag gtacggccaa catatctgat
     1921 accgagatcg gtcgccgcat tcacgattcc gagccgttgc atgatgtcgt ggatgagggt
     1981 gtctgtaccg gcggcgaatg cgcggcgttg aagttgaaga gcacgtagca gtctagaaga
     2041 ccgcggcttt aggttcgcaa acgcggcgtc gatctctgcc acgagggctt ggccgcgcgc
     2101 ttcctggccc agcagcgatg ccacttccct gatatgtttt ttcacgtctt cgattgatcg
     2161 cgctggcgaa aaggtggcaa tgcgaacgcc ctgggccgcg agctgcgcac gtgtggcgct
     2221 ccgcgtgaag cttcccgcca gcaccaaatc aggcttgagc ttcaaaacct cttccgcatc
     2281 gccgcggatc gtgggcagtc ccttggctcg gtccgcataa aacgacatgt ccggatcgcg
     2341 cgcataattc gtgagagcaa ggatttgctc ccgatccgcc agcgccagga tgagttgatc
     2401 ggcgcacgca tttat
//
LOCUS       DASUMG010000081         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_10681, whole genome shotgun sequence.
ACCESSION   DASUMG010000081 DASUMG010000000
VERSION     DASUMG010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10681"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /gene="trpE"
                     /locus_tag="VFX46_01280"
     CDS             complement(<1..1538)
                     /gene="trpE"
                     /locus_tag="VFX46_01280"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX5599248.1"
                     /translation="MAPGYAAFAERYEKGEPQVVSARLIADLETPVSAYLKLTSVPGR
                     DSAMTFLLESVEGGAVRGRYSIIGLKPDLVWRAFGETAEINTDPSTNPHAFERESLPT
                     LEALRALLARSAIPLPEDLPPMAAGVFGYMGYDTVRLIEHLPNPPRDALGVPDAILIR
                     PTLIVIFDSVKDEMVLVTPVRPVRGVSARAAYEAAQERLAGVAVALDVSLPRDALAVQ
                     DAAAELPEPVSNTTPAEYMAMVERAKEYIAAGDVFQVVLSQRFSIPFPLPPFALYRSL
                     RRTNPSPFLYHLDFGDFSLVGSSPEILVRVRRGEVTIRPIAGTARRGATAEEDQALAE
                     ALLADPKERAEHLMLLDLGRNDVGRVAEIGSVKVTERFVIERYSHVMHIVSNVIGRLD
                     PKKDAIDALMAGFPAGTVSGAPKVRAMEIIDELEKDKRGPYAGCVGYFSADGQMDTCI
                     VLRTALVKDGVMHIQAGAGIVYDSIPENEQQECINKAKALVRAAEDAVRLSSGAAQRQ
                     DRGNG"
     gene            complement(1580..>2832)
                     /locus_tag="VFX46_01285"
     CDS             complement(1580..>2832)
                     /locus_tag="VFX46_01285"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5599249.1"
                     /translation="EANKERFMTPEYRKLAIINLNIDEVKKRIPITEEELAAAYEHDK
                     ARYEVPERRRVLQLAFENRQDAEQAAAAIAQGKSFEEIAKERGIAEEDYTLGLLARRD
                     MIDSAVAEAAFSLENGAVSGVIDGRFSTVIVKVTEIQPGKQMSLDEVRDQVRDRLAQD
                     KASTELNRLYDEIDGRRFAGKPLKEIAQELGLPFMEIEATDRAGKAPDGSTALDVPDA
                     QRILNAAFQGQVGFEGDAVELADGGYAWVDVISVTEARQRSFEEAEADVTALWREQEM
                     RRRLSELGSKLAERAGQGESMEALAKEVGGKLETARNVKRLGGAPGLPDGAVGQAFIL
                     PEGGTGSAETRDGKSRVVFKVTEIIPAPPATPEELSQLRTELNRQLESDILTGYLTAL
                     QDRLGVSINEAAYRRAVGIASDQQ"
BASE COUNT          489 a          940 c          870 g          533 t
ORIGIN      
        1 ccgttcccgc gatcctggcg ttgagctgct ccgctggaga ggcgcaccgc gtcttctgcc
       61 gctcgcacca gggctttggc tttgttgatg cactcctgct gctcgttctc cgggatcgag
      121 tcgtaaacta tcccagcacc tgcctggatg tgcatgacgc cgtccttgac gagcgcggtt
      181 ctgagcacga tgcaggtgtc catctgccca tcggcggaga agtagccaac gcaacccgcg
      241 tatggcccgc gtttgtcctt ctccagctcg tcgatgatct ccatcgcccg cactttcggc
      301 gcgcctgaga cggtgccagc gggaaacccc gccataaggg cgtcgatggc gtccttcttc
      361 ggatcgagcc gcccgatgac gttcgagaca atatgcatca cgtggctgta gcgctcgatc
      421 acgaagcgct cggtcacctt gaccgagccg atctcggcca cgcggccgac gtcgttgcgg
      481 ccaaggtcca gcagcatgag gtgctcggcc cgctctttcg ggtcggcaag cagcgcctcg
      541 gccagcgctt ggtcctcctc cgctgttgcg ccgcggcgcg ccgtgccggc aatcggccgg
      601 atcgtcacct ctccgcgacg gacgcggacc aggatctccg ggctcgagcc gaccagagag
      661 aagtcgccga agtcgaggtg gtacagaaag ggcgaggggt tggtccgccg tagcgagcgg
      721 taaagtgcga atggcgggag cgggaacggg atcgagaacc gctgcgagag taccacctgg
      781 aacacgtctc cggcggcgat gtactccttg gcccgctcca ccatggccat gtactcggcg
      841 ggcgtcgtat tcgagaccgg ctccggcagc tcagccgccg cgtcctggac agcgagggcg
      901 tctcgcggaa gtgacacatc gagggcaacc gcgacaccgg caagtctctc ctgggcagcc
      961 tcgtaggccg cgcgggcgct gactcccctg acgggacgca ccggcgtcac caggaccatt
     1021 tcgtccttca ccgaatcgaa aatgacgatc agggtcggac ggatgaggat cgcgtcagga
     1081 acgccgagag catcgcgtgg tggattgggc aggtgctcga tcagccgtac cgtatcgtag
     1141 cccatgtacc cgaagacgcc ggccgccatt ggcggcagat cctccggcaa cgggatggcg
     1201 gagcgcgcaa gcagcgcgcg caaggcttcc agagttggca gcgactcgcg ctcgaacgcg
     1261 tgcgggttgg tgctcgggtc ggtgttgatt tccgccgtct caccgaatgc ccgccagaca
     1321 agatccggct tgagaccgat tatggagtaa cggccgcgga cggcgccgcc ttccaccgac
     1381 tcgagaagga aagtcatggc cgagtcgcgg ccaggcacgc ttgtcagttt gaggtaagcg
     1441 gagacaggcg tttcgagatc ggcgatcaac cgtgccgaga cgacctgcgg ttcacccttc
     1501 tcgtagcgtt ctgcgaaggc agcgtaccct ggcgccacat cgagcgcagc tttgaccggc
     1561 gtgctcacca gcaggcccct tattgctgat ctgatgcgat gcccacggcc cgccggtaag
     1621 cggcttcgtt gatgctcacg ccaaggcgat cctgaagcgc tgtcagatat ccggtgagga
     1681 tatcgctctc caactggcgg ttgagctcgg tcctcagctg agacagctcc tcgggagtcg
     1741 ctggaggtgc cggaatgatc tcggtgacct tgaagacgac gcgcgatttt ccgtcacgag
     1801 tctcagcgga cccagtacca ccctctggga ggatgaatgc ctggccaact gcaccatcgg
     1861 gtagcccagg agcgccacca agccgcttga cgttgcgagc cgtctccagc ttgccgccga
     1921 cctctttggc cagcgcctcc atgctctcac cttgaccggc gcgctccgcc agcttggagc
     1981 caagctccga cagccgacgc cgcatctcct gctcgcgcca gagcgccgta acgtcagcct
     2041 cagcttcctc aaaagaacgc tgccgcgcct ccgtcacgga gatgacgtcg acccaggcat
     2101 agccaccgtc agcgagctca acggcgtcgc cctcgaagcc cacttggccc tggaaggcgg
     2161 cattcaagat ccgctgtgca tccgggacat ccagagcggt tgacccgtca ggtgccttcc
     2221 ccgcccgatc cgtagcttca atttccatga agggcagacc gagctcctgg gcaatctcct
     2281 tgagcggctt gccagcaaag cggcggccgt cgatttcgtc atagaggcgg tttagctccg
     2341 tgctcgcctt gtcctgcgca aggcgatcgc gaacctgatc ccttacctca tcgaggctca
     2401 tctgcttgcc cggctggatt tcggtcacct tgacgatcac cgtcgagaag cgaccatcga
     2461 ttacgccgga cacggcacca ttctcgagcg aaaacgccgc ctccgcgacg gccgaatcga
     2521 tcatgtccct ccgcgccagg agacctagtg tatagtcctc ctccgcgatc ccgcgttcct
     2581 tcgcgatctc ttcgaagctc ttgccctggg cgatggccgc ggcagcctgc tccgcatcct
     2641 gacgattttc aaaggcaagc tgcagcacgc gccggcgctc gggaacttcg tagcgcgcct
     2701 tgtcgtgctc gtaggcggcg gccagctctt cctcagtgat cggaatgcgc ttcttcacct
     2761 cgtcgatgtt gaggttgatg atcgccagct tgcggtactc cggcgtcatg aagcgctctt
     2821 tgttggcctc gt
//
LOCUS       DASUMG010000082         5476 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_974643, whole genome shotgun sequence.
ACCESSION   DASUMG010000082 DASUMG010000000
VERSION     DASUMG010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974643"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="VFX46_01290"
     CDS             complement(<1..928)
                     /locus_tag="VFX46_01290"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5599250.1"
                     /translation="MSAANSTVVIASAARTPVGSFNGSLSSLPAHELGKVAIKAAIER
                     AGIEPGEVDEVIMGQILTAGAGQNPARQASIAAGIPVDSPAWGMNQLCGSGLRAVALG
                     AQQIQLGSSNIVVAGGQESMSQAPHCAHLREGTKMGDIQFIDTMIKDGLWDAFNGYHM
                     GSTAENVARQWQITREEQDRFAVASQNKAEAAKKAGKFKDEIAPVTVVTRKGETVVSE
                     DEYIREGTTYEAVAKLRPAFEKDGTVTAGNASGINDGAAAVVLMTAKEAKKRGLKPLA
                     RIASWATAGVDPKVMGSGPIPASRKALKKAGWT"
     gene            1165..1836
                     /gene="phaR"
                     /locus_tag="VFX46_01295"
     CDS             1165..1836
                     /gene="phaR"
                     /locus_tag="VFX46_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEX5599251.1"
                     /translation="MLSKNDPPAEKKEAVVIKKYANRRLYNTETSTYVTLEDLAAMVR
                     SDRDFVVYDAKTGEDLTHSVLTQIIVEQEGRSGGQTLLPIPFLRQLIRFYDDSIARMI
                     PSYLQMSLETLVKEQERFRAQFSHMLGQNPFGAAALDAMQEQARKNIAMFEKAMAMWS
                     PFGGAQGPDAKQEEKRSGSEKEDGGEPASKKASQSADKSEIEDLKAQLASMQERLEKL
                     SQKQS"
     gene            complement(1920..2333)
                     /locus_tag="VFX46_01300"
     CDS             complement(1920..2333)
                     /locus_tag="VFX46_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599252.1"
                     /translation="MSLRAIPLIAVALILYNAVVLLGGDATPNEILSSRLFEISMLHH
                     NTVWVFTWGDLLILVVLVLLFVELLKATYTTTASLLDHGLSMIVFVVCLVEFLLVPQA
                     ASSVFFFITMAALIDVVAGYTIGIRVARRDFQIGE"
     gene            complement(2473..2658)
                     /gene="rpmF"
                     /locus_tag="VFX46_01305"
     CDS             complement(2473..2658)
                     /gene="rpmF"
                     /locus_tag="VFX46_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX5599253.1"
                     /translation="MAVPRKKVSPMKRGFRRSADALKPPVHVEDKETGERRRPHHIDL
                     KTGKYRGRQILEPKNEV"
     gene            complement(2822..3511)
                     /gene="mtgA"
                     /locus_tag="VFX46_01310"
     CDS             complement(2822..3511)
                     /gene="mtgA"
                     /locus_tag="VFX46_01310"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEX5599254.1"
                     /translation="MAIIGGAAALAGFLAAFFIVVTFFLLLFRFIDPPMSTLMLGRAL
                     TGSNIEQHWVDLDDISPNLVRAVISSEDGQFCSHYGIDLREMEAALRQAGRDGFDDVR
                     GASTITMQVAKNLFLWPSKDLVRKGLELGIAVVMEQVWPKRRILEVYLNIAEWGPGIF
                     GAEAAARHHFGKSAAHLTAQEAALLAASLPNPHRRIAGRPGPHTRRIAQIVHARARTA
                     GARVSCVLPPR"
     gene            3719..4618
                     /locus_tag="VFX46_01315"
     CDS             3719..4618
                     /locus_tag="VFX46_01315"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX5599255.1"
                     /translation="MTLSQQLRAHADAVEARLRRILDSLSSDVPSRLADAMRYAVLGG
                     GKRFRPFLVLESARLFGLTSDDAVATAAAVELVHCYSLVHDDLPAMDDDELRRGRPTV
                     HIAFDEATAILAGDALLTLAFEVLAGAETHPDSKVRCELALGLARAAGAAGMVGGQQL
                     DLEAERLPRQEAHDLQHVRRIQRMKTGALIAFSAEAGAILASAPSEDRIALANYGHAL
                     GAAFQIADDLLDVEGTAETVGKTTGKDAAAGKATYVAALGIEGARQRLRELEAEAQAH
                     LDRFGARAEILRQAVAYAATRTR"
     gene            4690..5004
                     /locus_tag="VFX46_01320"
     CDS             4690..5004
                     /locus_tag="VFX46_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015633514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEX5599256.1"
                     /translation="MATFIMLTRLSHEATQSPASIKELSHKVMEHIRKDCPEVEWKKS
                     FAVLGPADYLDIFTAPDTESAMKVATVIRTFGHATTEVWPATEWDRFVDIISPMGGSS
                     GA"
     gene            5227..>5476
                     /locus_tag="VFX46_01325"
     CDS             5227..>5476
                     /locus_tag="VFX46_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599257.1"
                     /translation="MSEAMMGRVAIGVAVLAIIAAIWQYSVATSANDRAAAAEAARSA
                     LQSQLESVIAAREASDKALEEAKAQVTAAEQAKKDLEAQ"
BASE COUNT         1089 a         1737 c         1683 g          967 t
ORIGIN      
        1 tggtccagcc agccttcttc agtgccttgc gtgaagccgg gatcgggccc gagcccatca
       61 ccttgggatc gacgcccgcc gtggcccacg aggcgatgcg agcaagcggc ttcaggcccc
      121 gcttcttggc ttccttcgcc gtcatcagca cgaccgcggc agcgccgtca ttgatgccag
      181 aggcgttgcc ggccgtcacc gtgccgtcct tctcgaacgc tgggcgcagc ttggcgacgg
      241 cttcgtaggt tgtgccttcg cggatgtact catcctcgga gacgaccgtc tcgcccttcc
      301 gagtcacgac cgttacaggt gcgatctcgt ccttgaactt gccggccttc ttggcggctt
      361 cggccttgtt ctgcgaggcg acggcgaagc gatcctgctc ctcgcgggtg atttgccact
      421 ggcgggcgac gttctcggcc gtcgagccca tgtgatagcc gttgaaggcg tcccacaggc
      481 cgtccttgat catcgtgtcg atgaattgaa tgtcgcccat cttggtacct tcccgcagat
      541 gggcgcagtg gggcgcctgg ctcatgctct cctggccgcc ggcgacaact atattcgacg
      601 agccaagctg aatttgctgc gcaccgagcg caacggctct gaggcccgaa ccgcagagct
      661 ggttcatgcc ccacgcgggg ctgtcgacgg gaatgccggc ggcgatcgag gcctgacgcg
      721 ccgggttctg gcccgcaccg gccgtgagga tctggcccat gatgacctcg tccacctcac
      781 cgggctcgat gcctgccctc tcgatggccg ccttgatggc caccttaccg agctcgtgcg
      841 ctggcagact tgagagcgac ccgttgaacg agcccaccgg cgtgcgagcg gcgctcgcga
      901 tgacgactgt cgaattggct gcgctcattg tgcctctcct ggatgacccg gactggtgac
      961 gaatatcgac cctggtcgag gccgggccgc ccgtcgcgat ggaaactcca aacttccagc
     1021 acgatagcag cccgccgtcc tgacccgcaa tgcagcgtta ggtgcagtgc aacagaaaaa
     1081 cgcctatcta tgcaaatcag aggttgcctc gccagggtac tgtgctagct tgtgaaatag
     1141 aggcaatgtc acgatttgac cgccatgctc agcaagaatg acccacccgc cgagaagaag
     1201 gaggcggtgg tcatcaagaa atacgccaac cggcgtctct acaatacaga gaccagcacg
     1261 tacgtgacgc tggaggatct tgcggcgatg gtaaggtccg accgggactt cgtcgtgtac
     1321 gatgccaaga ccggcgaaga cctgacccac tcggttctta cgcagatcat cgtcgagcag
     1381 gaggggcgtt cgggggggca gaccctgctt cccattccat tcctgcgcca gctcatccgc
     1441 ttctacgatg acagcatcgc ccgcatgata ccgagctatc tccagatgag cctggagaca
     1501 ctcgtgaagg agcaggagcg tttccgcgct cagttctccc acatgctcgg ccagaaccca
     1561 ttcggcgccg ctgcgctcga cgccatgcag gagcaggccc gcaagaacat agccatgttc
     1621 gagaaagcga tggctatgtg gtcgccgttc ggaggcgccc agggtcctga cgcaaaacaa
     1681 gaagagaagc gatccgggtc ggagaaggag gatggcggtg agccagcctc caagaaagcg
     1741 agccagtctg cagacaagtc ggagatcgag gatctcaagg cccagcttgc ctccatgcag
     1801 gagaggctcg agaagctgtc acagaagcag tcgtaaccta agtgtagcga ccggcgaatg
     1861 taccgggagg cggcttcggc atcaggtgaa gcacctccct cgcctcttcg cttcttccct
     1921 cactcgccaa tctggaagtc acgccgcgca acgcgaatgc cgatcgtata gccggccacc
     1981 acgtcaataa gtgccgccat ggtgatgaag aagaacaccg agcttgccgc ctgcggcacg
     2041 agcaggaact cgaccaggca gaccacgaac acgatcatag acagcccgtg gtccagcaaa
     2101 gaggcggtgg tggtgtaggt cgccttcagc aactccacaa agagcagcac caggacgacc
     2161 agtatcaaca aatcgcccca cgtgaacacc cagacggtgt tgtggtggag catggagatc
     2221 tcgaacagcc gggacgacag aatctcgttt ggcgtggcgt caccgcccag cagcacgacg
     2281 gcattgtaaa ggatcaatgc cacggcaatc agcggaatgg ctctcagcga catcacgcac
     2341 ctccgattct ggaccgacca gctggcatgt agcgggtgcg cgggcggaaa ttatgtcagc
     2401 cagagaccaa acgcctcgct gccatagggt gtgagccgcc gccccccgca gtcggctctc
     2461 aaaaatagat gatcaaacct cgttctttgg ctcgaggatc tgccggccgc ggtacttgcc
     2521 ggtcttgaga tcgatgtggt gcggacgcct ccgctcgccg gtttccttgt cctccacgtg
     2581 gaccggcggt ttcaaagcgt cggcagacct gcggaagccg cgcttcatcg gtgaaacctt
     2641 cttacggggt acggccatgc ctgtctcctt cggtcggccg gcgccgagcg ggccgacgtc
     2701 gttctcatgt gatccaaacg ggtgaacgga atcccctcct gccagacaca atgggtccgg
     2761 cacacctcgc gcgcgcttat aaacggattt cgtgccaaac gctagcgtcc caagcggacc
     2821 ctcagcgcgg aggcaagaca caagacaccc gcgcgccagc agtgcgcgca cgtgcatgca
     2881 cgatctgggc aatccgtcgc gtgtgcggcc ccggccgtcc ggcaatacgc ctgtgcgggt
     2941 tgggcaaaga agcggcaaga agcgctgcct cttgggcagt caggtgggca gcagacttcc
     3001 cgaaatgatg ccgggcggcc gcctccgcgc caaagatgcc agggccccat tcggcgatgt
     3061 tgaggtagac ctcgaggatg cgacgcttcg gccagacctg ctccattacg accgcaatcc
     3121 cgagctccag tcccttccgc accagatcct tggatggcca cagaaacagg ttcttggcga
     3181 cctgcatggt gatggtgctc gcgccccgga cgtcatcgaa tccgtctcgc ccggcttggc
     3241 gcaaggcagc ctccatctcc cgcagatcaa tgccgtagtg agagcagaac tgcccatcct
     3301 cggagctgat caccgcgcgc acgaggttcg gcgagatgtc atccagatcg acccagtgct
     3361 gctcgatgtt gctgccggtg agcgcgcgcc cgagcatcaa cgtcgacatc ggcgggtcga
     3421 tgaaccgaaa caggaggaga aagaaagtta cgacaatgaa aaatgccgcc aggaatccgg
     3481 cgagcgcagc cgcgccacca ataatcgcca ccctggcgca ggacggtacg ttagctgcga
     3541 ggcgcgccgc ccaatcgtct gtccttatgc gcggctgaat cgccagcgcc tctcgtactc
     3601 tgtcctgcaa agccatactt ctggaccagc tcttcccggg gaagtgttat agcgtggcct
     3661 gcccccgaca gcagcaggca cgacttgggg acgtaaaccc ttcttaaatg gcgccggcat
     3721 gaccctctca caacagctcc gtgctcacgc agatgcagtc gaggctcggc tgcgacgcat
     3781 tctggattct ctgtcctcag acgtcccaag ccggcttgct gacgccatgc gttacgcagt
     3841 gcttggcggc ggaaagagat tccgtccatt tttggtattg gaaagtgctc gccttttcgg
     3901 cctgacgagc gatgatgcgg tcgcgaccgc agccgctgtc gagctggtgc attgctactc
     3961 tctcgtgcac gacgaccttc ccgccatgga cgacgacgag ctgcgccgag ggcggccgac
     4021 cgttcacatc gcattcgacg aagcgactgc cattctcgcc ggcgatgcgc tgctgacgct
     4081 cgcattcgag gtgctggcgg gcgctgagac gcaccccgat tcaaaggtgc gctgcgagct
     4141 tgcgttgggg ctggcccgag cggcaggggc tgctggaatg gtcgggggtc agcagcttga
     4201 tctcgaagct gagcgcctcc cccggcagga agcgcacgac ctccagcatg tacgacgcat
     4261 ccagcggatg aaaacgggtg cgctcattgc cttttcggcc gaagccggcg cgatactcgc
     4321 ctcagctccc tccgaggatc gaattgctct cgcaaattat gggcacgcgc taggtgccgc
     4381 gttccaaatc gccgacgatc tgctcgacgt cgaggggact gcggaaaccg tcggcaagac
     4441 gaccggcaag gatgccgccg ccggaaaggc cacatacgtc gcggcgcttg gcatcgaggg
     4501 cgcacggcaa cgcttacggg agctggaagc cgaagcccag gctcatctcg acaggttcgg
     4561 cgctcgcgct gagattctgc ggcaagctgt cgcatacgcc gctacccgca caaggtgagc
     4621 ttgtccaggg gatccgggcc tcaatcgttg gcgttcctgt ccagtccacg acggatagga
     4681 ggtgccacca tggcgacgtt tattatgctc acccggctga gccacgaggc cactcagtcg
     4741 ccggcctcca tcaaggagct cagccacaag gtgatggagc acatccgtaa ggattgcccc
     4801 gaggtcgaat ggaagaaaag cttcgccgtg ctcgggccgg ctgattacct cgacatcttc
     4861 acagcgccgg ataccgagtc ggcgatgaag gttgcgacgg tcatccgcac cttcggccat
     4921 gcgacgaccg aggtctggcc agccacggag tgggaccggt tcgtggatat catctcaccg
     4981 atgggtggct cgagcggtgc ctagccgagc atggctcccg ggggccgtcc gctcaccgcg
     5041 cccggcgccc tgccctcgac ccaagcccaa gcgagcaaac cttgcgagaa cgcacactcg
     5101 gatgccgcat cgaacaagcg ctgtggccag gtagtggcga gcctccggca caacgagtgg
     5161 aagcatgatt tttgatccgt gttgccctac actgcccaca gccatcgagc tggggagaaa
     5221 gcgatcatgt ccgaagcgat gatgggccga gtggccattg gcgtagccgt tctggcgata
     5281 atcgcagcaa tctggcagta cagcgttgcg acgtccgcaa acgatcgagc cgcagccgcc
     5341 gaggcggcgc ggtcggcctt gcaaagccag ctcgaaagtg tgatcgcggc tcgcgaggcg
     5401 tcagataagg cgctcgaaga ggcaaaggcc caggtcacgg ctgcagagca ggccaagaag
     5461 gacctcgagg ctcagc
//
LOCUS       DASUMG010000083         2114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1135131, whole genome shotgun sequence.
ACCESSION   DASUMG010000083 DASUMG010000000
VERSION     DASUMG010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135131"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VFX46_01330"
     CDS             complement(<1..768)
                     /locus_tag="VFX46_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5599258.1"
                     /translation="MAFPPERIICLTEETVETLYLLGEEARIVGVSGYAVRPPRVRKE
                     KPRVSAFISADIPKITALEPDLVLAFSDLQADIVAALIREGIAVHAFNQRTVAGILDM
                     IRLLAVMVGAHAKGEELATRLEKRLEAVADAAKSLPRRPKVYFEEWNEPMISGIGWVS
                     ELIQLAGGVDVFASHAARQSAKERIVTEEEVIAAGPDIIFGSWCGKKFRPEQVRARKG
                     FDAIPAVRNGNIYEIKSSIILQPGPAALTDGLDVIAEH"
     gene            926..1546
                     /locus_tag="VFX46_01335"
     CDS             926..1546
                     /locus_tag="VFX46_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX5599259.1"
                     /translation="MASPRIELHKGDLPAGLDLGPSVAVDTEALGLKPHRDRLCLVQL
                     SAGDNTAHLVQLKPGEYSAPRLKALLTDPAVLKIFHFARFDIAILKAYLGVTTAPLYC
                     TKIASKLARTYTDRHGLKDLVSEIVGVELSKQQQSSDWGAAELTQQQLQYAASDVLYL
                     HAVKARLDAMLEREGRQHLAQACFDFLSTRAELDLLGFDEIDPFAH"
     gene            1677..>2114
                     /locus_tag="VFX46_01340"
     CDS             1677..>2114
                     /locus_tag="VFX46_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599260.1"
                     /translation="MAIATDSNGRLLSAEPAAVAWSADADRERAFAAARRHSFIVRWL
                     KIILPVCAAAVLSSYAVFMQRSISVNVGAGNLELGPIAFSSEVLTMHNPRYEGYSKDG
                     SRFIVSARTAEQKVAGEGPIKLKTIEGSIVQPNKTTTTLQAASG"
BASE COUNT          433 a          655 c          618 g          408 t
ORIGIN      
        1 gtgctcagca atgacgtcca ggccatcggt cagagccgcg ggcccaggct gcaggatgat
       61 cgatgacttg atttcgtaaa tgttgccatt gcgaacggcc ggaatggcgt cgaaaccctt
      121 acgagcccgc acctgctcag gtcgaaactt cttgccgcac cacgaaccga agatgatgtc
      181 gggaccagcg gcgatgacct cttcctcggt gacaatccgt tctttcgccg actggcgcgc
      241 agcatgtgaa gcgaaaacat cgacgccgcc ggccaattgg atgagctccg aaacccaccc
      301 gatcccggag atcatgggtt cattccactc ttcgaagtaa accttgggac ggcggggcag
      361 tgacttcgcg gcgtccgcca ctgcctcaag gcgcttttct agccttgtgg caagctcctc
      421 tcccttcgca tgagccccca ccataacggc gagaaggcga atcatgtcga ggatgccggc
      481 aaccgtccgc tggttgaatg cgtgcacggc gataccttcc cggatgaggg ctgcgacgat
      541 gtctgcctga agatcggaga aggcaagcac gaggtccggc tcaagggcgg tgatcttcgg
      601 aatgtcagcg ctgatgaagg ccgagacacg cggcttttcc ttccgcacgc ggggcggccg
      661 aacggcatac cctgagactc cgacgatacg cgcttcctca ccaagcagat agagagtttc
      721 gaccgtctcc tcggtcaaac agatgatccg ctcgggcggg aaagccatgg gcacgcctcc
      781 gcgtgagtct cgtgcgtcgg caatcaatcg gcgatgccgg tgaggtagta aacccgcttg
      841 tttagggcca gaccgaatgc tacgactgtc aggtcaggct tcagcataag aaaagtcgtc
      901 gaagctcaat gacttaggga tcgagatggc cagtccgcgc atcgagctgc ataagggtga
      961 tcttccggcc ggactcgatc tcggcccgtc agttgctgtc gacacggagg cgctggggct
     1021 caaacctcac cgagaccggc tctgcctagt ccagctatcg gcgggcgaca acacagcgca
     1081 tctcgtccag ctcaagccag gtgaatattc cgccccgcgg ctcaaggcgt tgctgaccga
     1141 tcccgccgta ctcaagatct tccacttcgc ccgcttcgac atcgccattc tcaaggccta
     1201 tttgggcgtg accaccgcac cgctctactg caccaagatt gcttcaaagc tggctcgtac
     1261 gtacacggac cgccacggcc tcaaggatct cgtcagtgag atcgtcggtg tagaactctc
     1321 gaaacagcag caaagctccg attggggtgc tgctgagcta acgcaacagc agcttcagta
     1381 cgctgcctcc gacgtgctct atctgcatgc cgtcaaggcg cgtctcgatg ccatgctcga
     1441 acgggaggga cgacagcact tggcgcaggc ctgcttcgat ttcttgtcca cccgtgccga
     1501 gctcgacctg ctgggtttcg acgagattga tccgtttgcc cattaagggg ggccgggcag
     1561 ctcagtgctc gacagaatgt gcgtgatcct cggtcacgct tgccaccggt cccaatatct
     1621 ccctattttg ccgccagatt cacgcgaagc acggatcgat catccctgag cgcgcgatgg
     1681 ccatagcgac cgatagcaac ggccggctcc tgtcggccga accggcggcc gtggcttgga
     1741 gtgcggacgc agaccgcgag cgcgcgttcg cggcggcccg ccgccacagc ttcatcgtgc
     1801 gctggctgaa gattattctg ccagtctgtg ccgctgccgt cctgagctcc tacgccgtgt
     1861 tcatgcagcg ctcaatcagc gtcaatgtcg gagctggtaa cctcgagttg ggcccaatcg
     1921 ccttttcgag cgaggttctg acaatgcaca atcctcgcta cgaagggtac agcaaggacg
     1981 ggagccgctt cattgtgtct gcgcgcaccg cagagcagaa ggtggcgggg gagggaccca
     2041 tcaagctgaa aacaatcgaa ggcagcattg ttcagccaaa caagacgacg accaccttgc
     2101 aggcagcttc cgga
//
LOCUS       DASUMG010000084         4755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_332169, whole genome shotgun sequence.
ACCESSION   DASUMG010000084 DASUMG010000000
VERSION     DASUMG010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332169"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VFX46_01345"
     CDS             complement(<1..681)
                     /locus_tag="VFX46_01345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5599261.1"
                     /translation="MVDVAFAIPGDLNTPTGGYAYDREILARLPALGIRARHLQLAGG
                     YPEPSADDLSQTQALFAGLDLTNVLLVDGLAFGAIPEEIIRDVRVPIIALVHHPLGYE
                     PGLPEERAEQLIASERAALKFARRVIVTSPFTKRLLTQEFEVPPDLITVAMRGTAPAE
                     RASGSGDGQEIKLLSVGSITPRKGYPILVQALAPLSGLDWRLTIVGPQDRDRAAFAEL
                     QAAIARHKL"
     gene            complement(674..1078)
                     /locus_tag="VFX46_01350"
     CDS             complement(674..1078)
                     /locus_tag="VFX46_01350"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX5599262.1"
                     /translation="MYAVEVRDRIMIAHSLPDPFFGPAQNMHGATYVVDVAFFREELT
                     RHNVVVDIGAAHQVLHEVLKPLAYRNLDDLPEFKGKLTTTEFLCRYIFEAMAKAAREG
                     ALGEDGKTLARIRVTLHETDLARAWYEGPVSG"
     gene            complement(1092..1685)
                     /locus_tag="VFX46_01355"
     CDS             complement(1092..1685)
                     /locus_tag="VFX46_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX5599263.1"
                     /translation="METALNAVWDAGCGPADRIVVVGGGAVGMLVGYLCARMPGADVT
                     LVDIEPDRGQIAEQLGLRFALPSSTPTEADVVFHTCATPQGLAAAISAAGLEGTVVEL
                     SWYGEGNIPVPLGGAFHSRRLRLISSQVGEVAPSRRPRWTRRRRLEAALSLLQDPRLD
                     ALITEDIEFADAPLRLPQILAPGAPGVVSVIRYPAAS"
     gene            2148..3953
                     /gene="mdoH"
                     /locus_tag="VFX46_01360"
     CDS             2148..3953
                     /gene="mdoH"
                     /locus_tag="VFX46_01360"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HEX5599264.1"
                     /translation="MSDHHVLSSPMPVLGNRAVLTTPTGTQSMQVLRRRRLTMLLLNV
                     LTYALLMWGVGVVLSAGGWTVFDFVLLAAFAIGSPWSVLGFWNAAIGLWLLHGVRNPL
                     AEVAPFAKAGDSEVPILVRTAVLMTLRNEDPARAILRLKTIKESLDRTGYGGQFSYFV
                     LSDTSDPSIAAAEEEAVQAWKRSLSDPDQVVYRRRPENTGYKAGNLRDFCERWGGDYE
                     LMLPLDADSLMSGETILRMVRIMQAHPQLGILQSLVVGMPSGSAFARIFQFGMRHGMR
                     SYTMGQTWWAGDCGPFWGHNALVRIRPFAQKCQLPILPGKPPLGGHVLSHDQVEAALM
                     RKAGYEVRVLPVEGGSWEENPPTMLEFIRRDIRWCQGNLQYLKLLDMPGLQPMSRFQL
                     AWAILMFLGIPAWTLMIAFLPVLAWEARSISDYPTMLAIGLYVTFFTMYLSPKIAGLI
                     DAALTPGAVSRYGGRLRFAASALIELVFSFLLGAVSTIRTTIFMIGLLFGKSVTWGAQ
                     ARDVHGISWRAAVMAFWPQMLFGIVICGALLPISPAVLLWSLPLTAGYLLAVPFAVFT
                     AHPEFGRFLQRIGLCGIPEDFDPPPEIEALQSRCA"
     gene            3950..4726
                     /locus_tag="VFX46_01365"
     CDS             3950..4726
                     /locus_tag="VFX46_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5599265.1"
                     /translation="MTSAVQDKLSALKGDPRIGSLRRSLEIYYGNAARDAAMDALYAR
                     FIGPGDLAFDIGAHVGDRVGAFRRLGAKVVAVEPQPDCMYVLRALYKDDPEVTFVEAA
                     CSRKPGHLVLHVNSANPTVTTASRAFVSSASGAAGWEEQVWDREIEVETTTLDDLIRR
                     FGKPRFVKIDVEGLEADVIAGLSSPLPALSFEFTTIQREVALECIDRLLGLGPYRFEI
                     ALGESQVLKFGEWVSGYRMREFIRSLPHEANSGDVYAVAI"
BASE COUNT          863 a         1445 c         1468 g          979 t
ORIGIN      
        1 gagcttgtgg cgcgctatgg cagcctgaag ctcagcgaat gccgctctgt cccgatcctg
       61 cggccctacg atcgtcagcc gccaatccaa tcctgaaagg ggagccagag cctgcaccag
      121 aatcggatag cccttccggg gagtgatcga gccgacgctc agcagcttta tttcttggcc
      181 atctccgctg ccggacgccc gttcggcagg cgcagtgcct cgcattgcga ctgttataag
      241 atcgggcgga acttcgaatt cttgagtcag aagccgcttc gtaaacgggc tggtgacgat
      301 cacgcgacga gcgaacttca gggccgcccg ttcgctcgcg ataagctgct cggctcgctc
      361 ttccggcagt cccggctcgt aacctagggg gtgatgaaca agcgcgatga tcggcacgcg
      421 tacgtcgcga atgatctcct cggggatggc gccgaacgcc agcccgtcaa ctaggaggac
      481 attcgtcagg tcaagcccgg cgaagagtgc ttgtgtctgg gagagatcat ctgcgctcgg
      541 ctcaggatag cccccagcga gctgcaggtg ccgagcgcga attccgaggg ccggcaaccg
      601 cgcaagaatt tctcgatcgt aggcgtagcc gcccgttggc gtattcaagt cgccgggtat
      661 cgcgaaggcg acatcaacca cttacagggc cctcgtacca cgcgcgcgcc agatccgtct
      721 catgaagcgt cacacggatg cgggcgagag ttttgccgtc ttctcccagt gcaccctcgc
      781 gtgccgcttt ggccatggcc tcgaaaatgt agcggcaaag aaattcggtg gtcgtcagct
      841 tgcccttgaa ctccggaaga tcgtcgagat ttcggtaggc gagcggcttc aatacctcgt
      901 ggagcacctg atgggcagcg ccgatatcga cgaccacgtt gtgacgggtc agctcttccc
      961 gaaaaaaggc gacatccacg acgtacgtcg cgccgtgcat gttttgcgcc ggcccgaaga
     1021 acggatcggg caaactgtgg gcaatcataa tgcggtcacg cacctcgacg gcgtacatgg
     1081 cgatcctccg gtcaagaagc cgccggataa cgtatcacgg acacaacccc cggtgctcct
     1141 ggggcaagga tctggggcag gcgcagaggc gcatcggcga actcgatgtc ctcagtaatc
     1201 agggcatcca gccgcgggtc ttgcaggagc gacaaggcgg cttcaaggcg tcggcggcgt
     1261 gtccaccgcg gtcgccgcga gggtgccacc tctcccactt gtgatgagat gagcctcagc
     1321 cgccggctat ggaacgctcc gcccaacgga acgggtatgt tgccttcccc ataccagctg
     1381 agctcgacga ctgtcccctc caggccggct gcgctgatgg cagcggcgag cccttggggt
     1441 gttgcgcagg tgtgaaaaac cacgtcggct tcggtaggcg tactggacgg cagggcgaaa
     1501 cgaagaccga gctgctcagc aatctgcccg cggtcaggct ctatgtccac aagcgtcaca
     1561 tctgcccccg gcatccgggc acacaggtat ccaaccagca ttcccacggc gccaccaccg
     1621 accactacga tccgatcggc cgggccgcat cctgcatccc aaacagcatt gagcgccgtc
     1681 tccatattgg ctgccagggt ggctctcctt ggcggcactc cgtccggaag cggaacggcc
     1741 atgcacgcag gagctgtgaa cagatcctga tgtgggtgca acacgaaaac tgttcggcca
     1801 actagctccg atgggccggt ttcaacaacg ccggccgcgc aatagccgta tttaaccgga
     1861 aaagaaaatt tgccggcttg aaggggcgct cgcatgcgtt cctgttcgtt ttgagggacg
     1921 gttccggaca agatgaggcg ttccgtgccg cgactgattg cgctgaaaag cgtacgcaca
     1981 acgacgtcgt cacgagcagg ttccttcagc ggctcggcac gaagctccac ttcacctttg
     2041 cgcacatacc aaatggcatg ggccgttcgc gttctttctg ctgccgcttt tttcattttg
     2101 gacatccgtt cgacagctga ttaacggctg catccgaggg aagcatcatg agcgatcatc
     2161 acgtcctttc ttcgcccatg ccagtgctcg gcaaccgggc ggtgcttaca acgccgaccg
     2221 gcacgcagtc catgcaggtc ctgcgccgcc gccggctgac gatgcttctc ctcaatgtcc
     2281 taacgtacgc gctgctgatg tggggcgtcg gcgtcgtcct ttccgccggc ggctggaccg
     2341 tgttcgactt cgttcttctc gccgcgtttg ccattgggtc tccgtggtca gtgctcggat
     2401 tctggaatgc ggcgatcggg ctttggttgc tccatggcgt ccgcaatccc ttggccgagg
     2461 tagcgccctt cgccaaagcc ggcgacagcg aggtgcccat tctcgtgcgg acggcggtcc
     2521 tcatgacctt gcggaacgag gacccggcgc gcgcgatcct gcgcctcaag acaatcaaag
     2581 aaagcctcga tcgaaccggc tatggtggcc aattcagcta cttcgttctg agcgacacga
     2641 gcgatccctc catcgcggcc gccgaggagg aggcggtgca agcgtggaaa cgatcgcttt
     2701 ccgatccgga tcaggtcgtc tatcgccggc ggccggagaa tactggctac aaggctggca
     2761 acctgcgcga cttctgcgag cgttggggcg gcgactacga gctgatgctt ccgctcgacg
     2821 ccgacagcct gatgtcgggc gaaacgatcc tccgcatggt gcgcatcatg caggcgcatc
     2881 cgcaactcgg catcttgcag agcctggtgg tcggaatgcc gtcgggaagc gcctttgcac
     2941 ggatcttcca gttcggcatg cggcacggca tgcgctccta cacgatggga cagacgtggt
     3001 gggcggggga ttgcggaccg ttctggggcc ataacgcgct cgtccgaatt cgacctttcg
     3061 cgcagaaatg tcagctgccc atcctgcccg ggaaaccgcc gcttggcgga catgtgctgt
     3121 ctcacgacca ggtcgaggcc gcgttgatgc ggaaggccgg atacgaggtc cgcgtccttc
     3181 cggtggaagg cggctcgtgg gaggaaaatc ctccaacgat gctggagttc atccggcggg
     3241 atatccgttg gtgccaaggc aacctgcaat atctcaagct gctcgacatg ccggggctgc
     3301 agccgatgag ccgctttcag ctcgcctggg ccattttgat gtttctcgga ataccggcgt
     3361 ggacgctgat gatcgccttc ttgccggtgt tggcctggga ggcgcgcagc atttcggatt
     3421 atccaaccat gctggctatc ggcctatacg taaccttctt caccatgtac ctgagcccga
     3481 agattgcggg tttgatcgac gctgcactga cacctggtgc cgtgtcccgt tatggcggac
     3541 ggctgcggtt cgccgccagt gcgctcatcg agctcgtctt ttcttttctg ctcggcgccg
     3601 tctcgaccat tcgcacaacg attttcatga tcggtctgct tttcggaaaa tcggtcacct
     3661 ggggcgcaca ggcccgtgat gtgcacggca tctcgtggcg agcagcggta atggcgtttt
     3721 ggccccagat gctgtttggc atagtcatct gtggtgcttt gcttccgatc tcgcctgcgg
     3781 tcctcctatg gagcctcccg ctgacagctg gctatctgct ggctgtgccg ttcgctgtat
     3841 tcacggccca tcccgaattc ggacgtttcc ttcagaggat cggcttgtgc ggcataccgg
     3901 aggatttcga tccgccgccc gaaatcgagg cattgcagag caggtgcgca tgaccagcgc
     3961 tgtgcaagac aagctgtccg cccttaaggg cgaccctagg attggctcgt tgcggcgctc
     4021 tcttgagatc tattacggaa acgcggcgcg cgacgcggcg atggatgcgc tctacgcgcg
     4081 attcatcggt ccgggtgacc tcgccttcga catcggcgcg catgtgggcg atcgcgttgg
     4141 tgcgttccgt cgcctcggag caaaggtggt ggctgtcgag cctcagcctg actgcatgta
     4201 cgtgctccgt gctctttaca aggacgatcc agaggtcacg ttcgtggagg cagcctgcag
     4261 ccggaaaccg gggcatctcg tcctgcacgt caactccgcc aatccgaccg tcacgacggc
     4321 gtctcgcgct tttgtttcct cagcctcggg tgcagccggg tgggaagagc aggtgtggga
     4381 ccgggaaatc gaggtcgaaa cgacaacgct cgacgatctc attcgccggt tcggcaagcc
     4441 acggttcgtc aagatcgatg tcgaaggctt ggaggccgat gtgattgcgg gactcagcag
     4501 tccattgccg gcgttgtcgt tcgagttcac gaccatccag cgcgaagttg cactggagtg
     4561 catcgatcgg cttttgggtc ttggtccgta ccggttcgaa atcgcgctcg gagaaagcca
     4621 ggtgctcaaa ttcggcgaat gggtcagcgg ctacagaatg agagagttca ttcgctccct
     4681 tccgcatgaa gcgaattcgg gcgatgttta tgccgtcgcg atctgagtgt cagtgggcgg
     4741 cattggcctt cgccg
//
LOCUS       DASUMG010000085         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_975397, whole genome shotgun sequence.
ACCESSION   DASUMG010000085 DASUMG010000000
VERSION     DASUMG010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975397"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..379
                     /locus_tag="VFX46_01370"
     CDS             59..379
                     /locus_tag="VFX46_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5599266.1"
                     /translation="MPAKTFNAYGIEKAATEVAGILRALANERRLMILCKLVERGEDN
                     VNSLAETVGLSQSALSQHLAKMREEGLVTCRRESQTLWYRIADPRIEDLFATLHRLYC
                     RPLK"
     gene            401..946
                     /locus_tag="VFX46_01375"
     CDS             401..946
                     /locus_tag="VFX46_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HEX5599267.1"
                     /translation="MSIARISPLHAKRLLDQGAILVDIREADEHAREKIIGARHLALS
                     KLDEANLALPEGQPVIFHCKSGARTIANASRLAQSVGGAAEAFIIEGGLDAWKKAGMP
                     VATDRRQPLELQRQVQIGAGSLAFVGTMLGLFVSPWFFAVPAFVGAGLIVAGVTGFCG
                     MARVLMQAPWNRTTVDAARPN"
     gene            complement(1117..2091)
                     /locus_tag="VFX46_01380"
     CDS             complement(1117..2091)
                     /locus_tag="VFX46_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5599268.1"
                     /translation="MDRRRFVIGTAATALAAGSAFAQEVFPAHAITIINAFPPGGIND
                     LVTRPLAAAMEPILKQPVVVEAKAGAAGQVGAQFVASAKPDGYTLLSHNTGISGYAEV
                     DKLFGRPAKTTRADFIPLARLVADPVLLLVNDQQPYKTLKEFIDGAKARPDALVFSSG
                     GLYGATHLPLAYLEKATGPLKLRHLPTNGGGPAIVAILGNNAQVTTQSVTATLQHIKA
                     GKLRPLSIFSATRSKQLPDVPTLKELGYDVEYYLWVGIFAPKATPPAIVTTLRSAIGK
                     AIESDQFKTTLVNIGQELAYLDGPDFQKFWDIDGKRTDEAVIAIGRQG"
     gene            2409..2813
                     /locus_tag="VFX46_01385"
     CDS             2409..2813
                     /locus_tag="VFX46_01385"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5599269.1"
                     /translation="MDPGALVAWLPPGEMTGEIHEFDARAGGGYRMSLFYPPTERSSR
                     GKTSDKEDMVTVRFVELAPARRIVEGVKFVTTNPALFGEMTIEITFEPVSGGTEVTFL
                     CTNLPPGLRAEDNEAGSRARTRSYATRSPTRS"
     gene            complement(2768..3593)
                     /locus_tag="VFX46_01390"
                     /pseudo
     CDS             complement(2768..3593)
                     /locus_tag="VFX46_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016732987.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
BASE COUNT          662 a         1097 c         1179 g          655 t
ORIGIN      
        1 tcttgacata tatattagcg atgcctaatt aagcaatgcc ggattaagag ggcatgatct
       61 gccagcgaag accttcaatg cgtacgggat cgaaaaggcc gcaacggaag ttgcgggcat
      121 cctgcgcgcc ctcgccaacg agcggcggct gatgatcctg tgcaagctgg tcgaaagggg
      181 cgaggacaac gtgaactcgc tcgcggagac ggtcgggctt tcccagtccg ccctctctca
      241 gcacctggcg aagatgcggg aggaggggct ggtgacctgc cggcgggaga gccagacgct
      301 ctggtatcgc atcgccgacc cgcgcatcga ggacctcttc gccaccctgc acaggcttta
      361 ttgcaggccg ctcaagtgac gctgaacaac ggagttagcc atgtctattg ccagaataag
      421 cccgcttcac gccaagcgcc tcctcgatca gggggcgatc ctggtcgaca ttcgtgaggc
      481 cgatgagcat gcccgggaaa agatcatcgg ggctcgccac ttggcgctgt ccaagctgga
      541 tgaggcaaac cttgcgctgc ccgaaggcca gccggtcatc ttccactgca agagcggcgc
      601 ccgcacgatc gccaatgcgt cccggctggc gcagagcgtg ggcggcgcgg ccgaagcctt
      661 catcatcgag ggcggtctcg acgcatggaa gaaggcgggg atgcccgttg cgaccgatcg
      721 ccgtcaacca ttggagttgc agcggcaggt tcaaatcggc gccggcagtt tggccttcgt
      781 cgggacgatg ctgggcctgt tcgtttcgcc gtggtttttt gccgtgcccg ccttcgtggg
      841 cgctgggctg atcgtggcag gcgtgacggg gttctgcggc atggctcgcg tgctcatgca
      901 ggcgccatgg aaccggacga ccgtggacgc tgctcgacca aactaaatcg tcagccgaca
      961 tcgaaggcga tgtggggcac ctacgatggt tttgtcgatg actcaaacgg ctcttgccgt
     1021 cgctcacgtc tccgccaagg acgaatccgg acgcagacgt gccgccgtca ctccgctgga
     1081 ctcatgacgc catattcggg tgatcctcga gtcaatctat ccttgtcggc cgatcgcgat
     1141 caccgcttcg tcggtccgct tgccgtcgat gtcccagaac ttctggaagt ccggtccgtc
     1201 gagatatgcg agctcctggc cgatgttcac cagcgtggtc ttgaattgat ctgattcgat
     1261 cgccttgccg atggcggagc gcagcgtcgt cacgatcgcg ggcggggtcg ccttgggcgc
     1321 gaagatcccg acccacagat agtattcgac gtcgtagccg agctccttga gcgtcggcac
     1381 gtcgggcagt tgcttggagc gcgtagcgct gaagatggac agcggtcgca gcttgccggc
     1441 tttgatgtgt tgcagcgtgg ccgtcaccga ttgcgtcgtg acctgggcat tgttgccgag
     1501 gatggcgacg atggccgggc ctccaccatt ggtcgggaga tggcgcagct tcaatgggcc
     1561 ggtcgccttc tcgaggtaag ccagcggcag atgagtcgca ccgtagagac cgcccgaact
     1621 gaacacgagc gcgtccgggc gcgccttggc gccgtcgatg aactccttca gcgtcttgta
     1681 gggctgctgg tcgttgacca ggagcaggac cggatccgcg accaggcgcg cgagcggaat
     1741 aaagtcggcg cgggtggtct tggccggccg gccgaacagc ttgtccacct cggcgtagcc
     1801 ggagatgccg gtgttgtgcg acagcaatgt gtatccgtcc ggcttggcgg aggcgacgaa
     1861 ctgcgcgccg acctgtccgg cggcgccggc cttcgcctcg accaccaccg gctgcttgag
     1921 gatcggttcc atggccgccg ccagcggccg cgtcacgaga tcgttgatgc cgcccggcgg
     1981 aaacgcgttg atgatcgtga tggcgtgcgc gggaaaaacc tcttgcgcga atgccgatcc
     2041 ggccgctagc gcggtcgcag cggtcccgat gacgaagcgg cggcgatcca tgtgttgccc
     2101 tcttgaagcg gtttcctcgg atgactccca aaatcggcga cagtattcgc gcatgtcgcg
     2161 ggtcccgcaa gacggatcga tgccgcaggc gctaccccaa ccggaaaagc ctcgcccgag
     2221 agggggggcg cgcaggctcg tcgagggagc gtcggccgac ctgttgaccg gctcgaccat
     2281 ctcatgttca ggggagtcaa ttcccagcgc attgacggag ccaccacatg accgcagcgc
     2341 ataacggcgc ctggacccgt acatcgcgga tcatcaaggc ccggccggag gagctctacg
     2401 cagccttcat ggaccccggc gcactcgtgg cttggcttcc gccaggggag atgacgggcg
     2461 agattcacga gttcgacgcg cgggccgggg gtggatatcg gatgtcgctg ttttaccccc
     2521 cgactgagcg ctcctcacgc ggcaagacgt ccgacaagga ggacatggtc acggtgaggt
     2581 tcgtcgaact ggcgccagcg cgcaggatcg tcgaaggcgt caagtttgtc accaccaacc
     2641 cggccctgtt cggcgaaatg acgatcgaga ttacgttcga accagtgtcc ggcggaaccg
     2701 aggtcacctt cctgtgcacg aatctgccgc ccggcttgcg ggccgaggac aacgaggccg
     2761 gctcgcgcgc gcgcaccaga tcgtacgcca caaggtcgcc gacgcgctcg taaagcgcgg
     2821 tggaaccctt gaggtcggtg aacaggaagg tgaggcttgt gatcttgagc ccctggtcca
     2881 cgtcgagcgt gtcggtacga tagatgtcgc ggaaggtctg gttcgagagc aaacggttgg
     2941 cggtcaggaa cggctttcgc ttgccgagca agtcatgcag cgtgtcgccc gcgatccaca
     3001 gcgacggcag cacacgcacg tcggtccggt tatcgagcga aagccgcagg ggtcccggcc
     3061 tcagcaccat ggtgccggta ggggcgcgga ccttgttgta agtgagcatc aaagtctgac
     3121 gctcacgggt cggttccccc ttcacatcga ggaactgtgc cgcatgcgtg acgggctcga
     3181 agacgatcac gaactccgcc ggcaactgca cggagacaaa ggcccgctcc ccggggggaa
     3241 gctcgaccga atcgatcgag atttcgtcga tcagctcttc aaattctcgg gaagatcgat
     3301 gcccgaactc cagaagatct ggcgaaagta ttcccagatc gggagcgtat tcggatcatg
     3361 cgcggcgatt ctgcgcacct gcgggcttac ggtgaaggtg acctccacca tctcgtccag
     3421 agtgggctcg tagccggcgg cacagagtgc gcactcgtag ctctccttgt gcacgctttt
     3481 cagggtagca gttgcgtcga ggaccccacc gcagcccgga cataggacgt tccacgacag
     3541 ctcgaagagt ccaacccgag ctgcatgcag gaacgccgcg atcacccgct ctt
//
LOCUS       DASUMG010000086         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_895433, whole genome shotgun sequence.
ACCESSION   DASUMG010000086 DASUMG010000000
VERSION     DASUMG010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_895433"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VFX46_01395"
     CDS             <1..348
                     /locus_tag="VFX46_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HEX5599270.1"
                     /translation="EIYTTRLACRLLDLGTCRCTHYAERQKHVPDCVSITPEKVRTLN
                     WLPETCAYRVLAEGRDLAWWHPLVSGDPETVHQAGISVRGWVKSEERASPETVHRYII
                     KDWGRPRRRVRTT"
     gene            504..1772
                     /gene="lysA"
                     /locus_tag="VFX46_01400"
     CDS             504..1772
                     /gene="lysA"
                     /locus_tag="VFX46_01400"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX5599271.1"
                     /translation="MHHFSYRGGVLHAEEVSLARIAEEVGTPFYCYSTATLERHYRVL
                     SEAFAGTDHLICFAVKANSNQAVLRTMARLGAGMDVVSEGELRRARAAGVPADKIIFA
                     GVGKTRQELAYAVEEGVLGFNVESEPELEALSEVAAQKGKTARIAIRVNPDVDARTHA
                     KIATGKSENKFGIPYLDAPRLYAQAARLPGIKIAGIHMHIGSQITDLMPFRDAFRLMR
                     ELVIDLRREGYELEHLDIGGGLGVPYRGTNDLPPHPDEYAALVKAALGDLGLKIILEP
                     GRMIAGNAGVLVSRVIYSKVGAAKTFAIVDAAMNDLIRPTLYEAYHDIWPVREAKLNL
                     ERQVQDVVGPVCETGDYLALDRALPPLEPGDLIAVMTAGAYGAVMSSTYNSRLLVPEV
                     LVRGDQYAVVRPRPTYDDLIGLDKIPDWLT"
     gene            1906..>3021
                     /locus_tag="VFX46_01405"
     CDS             1906..>3021
                     /locus_tag="VFX46_01405"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX5599272.1"
                     /translation="MFSKQRSTTTESRAFERKVRLSKLALLFERLWPRLWLVIGVLGL
                     FVLVSFAGLWLWLPPIAHEIVLGAFGVALLAALIAVARVPFPTRDEAIRRIERRSGIP
                     HRPASSYEDTLTIPSGNSATERIWQAHKARLAALLDKLRVGAPQPRTDHKDPFALRAL
                     LILTVVLLMGLVGDSAKDRLLAAFRFSPEVQLADARLDAWVTPPAYTGRPPILLADGG
                     AGMLRPAAEESNLIEVPDKSLLVIRASGAGQLSLEIAAAEAEETQTVEGQAPKQAGDV
                     SEVRAELRNSGTVRVLGNGSELVAWKFKVIPDQPPRIVVTKEPESTIRGGIKLFYKVE
                     DDYGVASAEARFARPRTESGDPRTRWARADLLKGPRPP"
BASE COUNT          542 a          939 c          954 g          586 t
ORIGIN      
        1 gaaatctaca cgacccggct tgcctgccgc ctactcgatc tcggcacgtg ccgctgcacg
       61 cattacgcgg agcggcagaa gcacgttcct gactgcgttt ccattacgcc cgagaaagtg
      121 cggacgttga attggctgcc cgagacgtgc gcctacaggg tgctcgcgga gggtcgcgac
      181 ttggcctggt ggcatcccct cgtctctggc gacccggaaa ccgttcacca agccggcata
      241 tcagtccgcg gctgggttaa gagcgaagag cgcgccagtc cggagacggt gcaccggtat
      301 atcattaagg attggggccg cccgcgccgt cgcgtccgca ctacgtaatc atttccttaa
      361 ttgggaccgt cggatcatcg tattgcgtcc gtcaccttaa ttcaccagcg tcgcgggcgc
      421 acaaagcgca cttgactggc gtacgaaggc cagcgcatag cgagaccggc gggccggtgg
      481 cggcctcaga ggcgcgcgga tttatgcatc atttcagcta tcgcggcgga gttctgcacg
      541 ccgaggaagt cagcctcgcg cgtatcgccg aggaagttgg cactcccttc tactgttatt
      601 cgaccgctac tctggagcgg cactaccgcg tgctgtccga ggcttttgcg ggaaccgatc
      661 acctgatctg ctttgcggtg aaggccaact caaaccaagc tgtcctccgg acgatggcgc
      721 ggctgggcgc agggatggat gtggtctccg agggagagct gcgccgcgct cgggccgctg
      781 gagttcctgc agacaagatc atcttcgctg gggttggtaa gacgcggcag gagctcgcct
      841 atgccgtgga agaaggcgtg ctcggcttca acgtggaaag cgagcccgag ctggaggctc
      901 tgagcgaggt agcagcgcag aaaggcaaaa ccgctcgcat cgccatccgc gtcaatccgg
      961 atgtcgacgc gcgcacgcac gccaagatcg cgactgggaa gtcagagaac aaatttggca
     1021 tcccctatct cgacgcgccg cgcctttatg cacaagccgc ccggctgccc ggcatcaaga
     1081 tcgccggcat ccatatgcat atcggcagcc agatcaccga tctcatgccg ttccgggacg
     1141 ctttccggct gatgcgggag ctggttatcg acctccgccg ggaaggctac gagcttgagc
     1201 atctggatat cggaggagga cttggggtgc cctatcgcgg cacaaacgac ttgccgcccc
     1261 atcccgacga gtatgcggcg cttgttaagg ccgcgttagg cgacctgggc ttgaaaatta
     1321 tcctcgagcc tggccgcatg atcgctggca acgccggtgt gctcgtctcc cgggtcatat
     1381 attccaaggt cggtgcggcg aaaacctttg ccatcgtcga tgccgccatg aacgacctca
     1441 tccgcccgac gctctacgag gcctatcacg acatctggcc cgtgcgcgaa gcgaagctga
     1501 atctggagcg tcaagtgcag gacgtcgtcg gtccagtttg cgaaacgggc gactacctgg
     1561 ccctcgaccg tgctttgccg ccgctcgagc cgggcgacct tattgccgtc atgacggcgg
     1621 gcgcgtatgg tgccgttatg tcgtcaactt acaattccag gttgctggtg ccggaagtcc
     1681 tggttcgtgg cgatcagtac gccgtcgtac gcccgcgtcc gacttacgac gatttgattg
     1741 gcctggacaa aattccggat tggctcacct aggcttgatc caggctgccc ttgtcgggcc
     1801 tttttctacc ctgcaccatg caacccggca ctggatccgg gtgttagggc agacggacgc
     1861 gcaaccatcc gatgcacgca gccaatgtcg tgctaggcta gaggcatgtt cagcaagcag
     1921 cgttccacaa ccacagaaag ccgcgccttc gagcgaaagg tccggcttag caagttggcg
     1981 cttctcttcg agaggctctg gccgcgcctc tggctggtta ttggcgtgct cggcctgttc
     2041 gtgctcgttt cgttcgccgg tttgtggctg tggctgccgc ccattgcgca cgagatcgtg
     2101 ctcggcgcct tcggcgttgc cctgctcgct gcgctgatcg cagtcgcccg ggtgcctttc
     2161 ccgacccgtg atgaggccat tcgacgcatc gagcgccggt cgggcatccc tcaccggccc
     2221 gcctcctcct acgaggacac gctgacgatt ccgagcggca actccgctac ggagcgcatc
     2281 tggcaagcac acaaggctcg gttggcagcg ctgcttgaca agctcagggt cggagcacct
     2341 cagccgcgaa ccgatcacaa agacccattc gcccttcggg ctttgctgat tctcaccgtc
     2401 gtgctgctta tgggcttggt cggtgacagc gcgaaggaca ggctgctcgc cgcttttcgc
     2461 tttagcccgg aagtgcagct tgcggacgcg cgtctcgacg cctgggtgac gccgccggct
     2521 tacactgggc ggccgcccat tcttctcgcc gacggtggcg ccgggatgct gcggcccgct
     2581 gctgaggaaa gcaatctcat cgaggtaccg gacaagagcc ttctggtcat acgggcgagc
     2641 ggtgccggcc agttgtcgtt ggaaatcgca gcggcggagg cggaggagac gcaaacggtc
     2701 gaaggtcagg ctccgaagca ggcgggcgat gtctctgagg ttcgcgcgga gctccggaat
     2761 tcgggcacag ttcgcgtact cggcaacggc agcgagcttg tggcctggaa attcaaggtc
     2821 attccggacc agccaccgag aatcgtcgtc accaaggagc cggaaagcac gatccggggc
     2881 ggcatcaagc tgttctacaa ggttgaggac gactacggcg tggcctcagc ggaggcgcgc
     2941 ttcgcgcgtc ctcgcacgga atccggcgat ccacgcacgc gttgggcaag agctgatctt
     3001 ctgaaggggc cgcggcctcc a
//
LOCUS       DASUMG010000087         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_172498, whole genome shotgun sequence.
ACCESSION   DASUMG010000087 DASUMG010000000
VERSION     DASUMG010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172498"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..729)
                     /locus_tag="VFX46_01410"
     CDS             complement(<1..729)
                     /locus_tag="VFX46_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX5599273.1"
                     /translation="MRRIGEVAAIALAALIAGFLGPAEAKEKVKVGFIGPLTGGNSVI
                     GLGGRNSADLAVRLRNQDPNAKYEYELVVLDDECKPNVAVQVATKMSTDRSIIAAATH
                     YCSATAIATVDVYHKFNFPIIVWGAVLPDITYRNKYPEVHRVNGTMINQNEVNAELLT
                     SLGYKTFAVIHDTTDYGKGHNKYFSEALEKRGGKILGTFGVTADQQDFTAELTQIKAL
                     DPEVIYFGGLTPIGVRIRSQMDKLG"
     gene            913..1716
                     /locus_tag="VFX46_01415"
     CDS             913..1716
                     /locus_tag="VFX46_01415"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX5599274.1"
                     /translation="MTPFIIAGDISAFSAALAERLAVVLPNAIVAFVMLAVGWALAGW
                     AERGTGRLMERARIDPTLQGITAAVVRYGLLIVVIVAALGQLGVQITSILAVLGAIGL
                     AIALALQGTLANIAAGIMLLWLRPFRVGDYIQAGDIAGTVKTVGLFTSELHSADGVYQ
                     SVPNSELWNKRITNYSRLERRLVDVRFLIAYSNDIETARRALLDLAQSDNRVLEDPPP
                     SVFVDQFAENYILLGLRAWVATANHWAVRCALLEGGKARLEAAGVRIPK"
     gene            1773..2168
                     /locus_tag="VFX46_01420"
     CDS             1773..2168
                     /locus_tag="VFX46_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEX5599275.1"
                     /translation="MAKAIHSMIRVLDEKRSVDFYAKAFGLQIADRFQFDDFTLIYLR
                     NDEADFELELTVNHGRTEPYDLGTGYGHIAFAVDDLEAVHRRFEREGLNPTPIKELSH
                     QGKPLAKFFFVTDPDGYKIEVLQKQGRYS"
     gene            complement(2278..>3219)
                     /locus_tag="VFX46_01425"
     CDS             complement(2278..>3219)
                     /locus_tag="VFX46_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5599276.1"
                     /translation="SVFGWNVPHGPLGEALWRAVQQHPRITVVETARVEHVVIGSDEV
                     VARTAEGEELKARLVAAADGRNSICRAAAGITTTKWDYDQAAIATRFEHTRPHGNVST
                     EFHRKSGPLTTVPLPGLASSLVWVERPAIAKRLAELDDDGFRVVLETRLQGLLGRVGE
                     LGPRRLFPLSALTAEAYARSRVALIGEAAHVIPPIGAQGLNLGLRDAATLADCVAWAL
                     ARGEDIGGDAVMRQYHERRKTDVESRIMAVDALNRTLLSTLLPVSLLRGFGLHLLRAV
                     GPLKRALIRQGLEPAGPRPHLMEPGGRVAQAAVAGGR"
BASE COUNT          602 a         1016 c          962 g          639 t
ORIGIN      
        1 gccgagcttg tccatctgcg aacggatacg caccccgatc ggagttaaac cgccaaagta
       61 aataacctcc ggatcgagcg ccttaatttg ggtcagctcg gccgtgaagt cctgctgatc
      121 agcggtaacg ccaaaggtgc cgaggatttt gccaccccgc ttctccagcg cctcgctgaa
      181 atacttgttg tggcctttgc cgtagtccgt cgtgtcgtgg atgacggcga aagtcttata
      241 gccgaggctg gtcagtagct cggcattcac ctcgttttgg ttgatcatcg tcccgttgac
      301 gcggtgcacc tcgggatact tgttgcggta ggtgatgtcg ggcagcaccg ctccccatac
      361 gatgatcgga aaattgaact tgtgatagac gtcgacagtg gcgatggccg tggccgagca
      421 atagtgggtc gccgccgcga tgatgctcct gtcggtcgac atcttggttg cgacctggac
      481 agcgacgttc ggcttgcatt cgtcgtcgag aacgacgagc tcgtattcgt acttcgcgtt
      541 cgggtcctga ttgcgcaggc ggacggcaag atcggcagag ttacggccgc ccagtccaat
      601 caccgagttt ccaccagtca gcgggccgat gaagccgacc ttgacctttt ctttcgcctc
      661 ggctggccca aggaaacctg cgatcaacgc ggccagcgcg atggcagcta cctccccgat
      721 cctacgcata atccttgctc ctatatcgca tttcgcgggc ggagtattag atgaaacggc
      781 aacatttaca agcgggaagg cacgttaagc tgagcgccgt ataacgacac cccgcccttt
      841 cggaaggaca tcgtgctatg cccatcgctc gcctcttcaa gcgaatgaac gggcttgtga
      901 gtgaggagtg aaatgactcc attcataatc gctggagaca tcagcgcgtt ctccgcagct
      961 ctggcagagc gcctggcggt ggtgcttccc aacgccatcg tggcattcgt catgctcgcg
     1021 gtcggttggg ctctggccgg atgggctgag cgcggcaccg ggcgcctcat ggagcgggcg
     1081 cgcatagatc caacgctgca aggcattacg gccgccgtgg ttcggtacgg cctgctgatc
     1141 gtcgtcatcg tggccgcgct tggtcagctc ggggtgcaga tcaccagcat cctggccgtg
     1201 ctgggtgcca tcggccttgc cattgccctc gcactccaag gaacgctggc caatatcgcg
     1261 gcaggcatca tgctgctttg gctccggccg ttccgggtcg gcgattacat ccaggcaggc
     1321 gatatagccg gaacagtaaa gaccgttggg ctgttcacgt cggagcttca ttctgcggac
     1381 ggggtttatc agtccgtgcc gaactcggag ctttggaaca agcggatcac caattactcg
     1441 cggttggagc ggcgcctcgt ggacgtgagg ttcctgatcg cctactccaa cgatatcgaa
     1501 acggcgcggc gggctcttct cgatctcgct cagtccgaca atcgcgtgct cgaggatcca
     1561 ccgccatccg tcttcgtcga ccaatttgcc gagaactata tcttgctcgg cttacgggcg
     1621 tgggtggcga cggcgaacca ttgggccgtc cgctgcgcac tcctggaggg cggaaaggcg
     1681 cggctcgagg cagcgggcgt ccgaatccca aaataggcgc acatttgatc caaaccgcga
     1741 ggtgaaaccg aatttctggt tggaggcatt cgatggcaaa agcaattcac agcatgatac
     1801 gagttctcga cgaaaagagg tccgtcgatt tctacgccaa ggcttttggc ctccagatcg
     1861 cagaccgttt ccaattcgat gatttcaccc tcatctatct gcgaaatgac gaagccgatt
     1921 tcgagctcga gctcacggtg aaccacggcc gaacggaacc ttatgatctc ggaacgggct
     1981 acgggcacat cgcgtttgct gtcgatgact tggaggcagt gcacaggcgc ttcgagcgcg
     2041 agggccttaa tccgacgccg atcaaggagc tttcacacca gggaaagcct ttggcgaaat
     2101 ttttctttgt gacagacccc gacggttaca aaatcgaagt gctgcagaag caaggtcgct
     2161 atagctgaat tttctctagc ccgaattcgt cgcttctggc tttgaaatcg aaatggcgtt
     2221 ttcgaccgat gtgatcggaa acgcccaatt cttgctttcc caatcgggcg ccgcagatca
     2281 gcgtccaccg gccacagccg cctgggccac cctgccgccc ggctccatga ggtgcggccg
     2341 tggcccggca ggctccaggc cctggcggat cagcgcgcgc ttcagcggtc caacggcgcg
     2401 cagcaggtgc aggccgaatc cgcgcaagag gctcacgggc agcagcgtgc tgagcaacgt
     2461 ccggttgagc gcgtccacgg ccatgatgcg gctctcgaca tccgtctttc tgcgctcatg
     2521 gtactgacgc atgaccgcgt cgccgccgat gtcctctccg cgcgccaacg cccatgcgac
     2581 gcagtcggcc agggtggcgg catcccggag cccgaggttc aacccttgcg ctccgatcgg
     2641 cgggatgacg tgtgccgctt cgccgatcaa agccacgcgg gaccgcgcgt acgcctccgc
     2701 cgtcaaagcc gaaagtggaa agagccgccg cggccccagc tccccaacgc ggccgagcag
     2761 gccctggagc cgcgtctcca gcaccacccg gaagccatcg tcgtcgagtt ccgctagccg
     2821 cttggcgatc gccggccgct cgacccagac gaggctcgaa gcgagacccg gcagtggcac
     2881 cgtcgtcagt ggtcccgact tgcggtggaa ctcggtcgac acgttgccat gcggccgggt
     2941 gtgctcgaag cgagtcgcga tggccgcttg gtcataatcc catttcgtcg tggtaatccc
     3001 cgccgccgcg cggcaaatgg agttccgccc gtccgccgcc gcaacgaggc gcgccttaag
     3061 ctcctcgccc tccgccgtcc gcgccacaac ttcgtcgctg ccgatcacga catgctcgac
     3121 ccgcgcggtc tccaccaccg taatccgcgg gtgctgctgc accgcgcgcc acagagcttc
     3181 acccagcggc ccatggggca cgttccagcc gaacacgct
//
LOCUS       DASUMG010000088         3430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1136590, whole genome shotgun sequence.
ACCESSION   DASUMG010000088 DASUMG010000000
VERSION     DASUMG010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136590"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..818)
                     /locus_tag="VFX46_01430"
     CDS             complement(93..818)
                     /locus_tag="VFX46_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5599277.1"
                     /translation="MGAAFAGAFVQSAVGFGFALITAPIFLTAMQSGSAMQVLVAIHV
                     VQSAMLVPGLWREAPKELLSPMLVGSLVGFPAGLFVFLALDLDTLKLTVGVVMLVFTA
                     LLIAGERSWLSHLFVVRGPPHPAAAATVGASAGALTSVLVMPGPPLILYVAARRLGKL
                     ESRALSLTFFAFCYVMVTLLHAVWTGMSAETWAAAGALTPAVIIATLLGNRLAHRLSE
                     ARFRAAVLAISFLSGVWAIWSSL"
     gene            complement(852..1217)
                     /locus_tag="VFX46_01435"
     CDS             complement(852..1217)
                     /locus_tag="VFX46_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX5599278.1"
                     /translation="MFAVGFPSWPYLAPLLLLVASNVFMTFAWYGHLKFKNAPLLVVI
                     LGSWSIAFVEYCLAVPANRIGHSVYSAAELKTMQEVITLAIFAGFSVLYLGEPLTWRH
                     AAGFALIALGAAFVFSGRA"
     gene            1384..2268
                     /locus_tag="VFX46_01440"
     CDS             1384..2268
                     /locus_tag="VFX46_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5599279.1"
                     /translation="MRRLKADLLLLLAAAIWGLAFYFQKTAMEHIGPFLFIAARGVIA
                     AVCLLPLAFFEAARVTIPWSVGFVQVGLAGGLAFLVAAALQQTGIIDSTVTNAGFLTG
                     LYVVATPLLSWVLFGERPAAHVWVAVPLAFLGVWLLGGGTIGGLSKGDALLAAAALVW
                     ALHIIITSRASVYGRPVAYTAMQFAFVAFGGGVLALAIESNTLEGFYKALPAITYVGV
                     LSSALTFTLLAIALKDTPPTEASILMSTETLFAAAAGVLLANDWLPPIGWIGAALMFA
                     ATVLVQIGPAMEGPKKSV"
     gene            complement(2679..>3430)
                     /locus_tag="VFX46_01445"
     CDS             complement(2679..>3430)
                     /locus_tag="VFX46_01445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599280.1"
                     /translation="GGWSITHLGWRPAFSVMCVLCLGLAVPLLLAPRHYNTARPLQPF
                     RMGAIFALSNFLRPFRAMSLAPGLTQLALACTGFAMVQSACFSFFVIYLHAGLGYGLA
                     LAGTLFAVLQAASVVGRIVFGYLADRIGSPRPVLMVLAGCSAGSALLLAALGSDHCPL
                     LLFTVAGLAGASVATWNGLYLAEVANLAPAHSVGEATAGTTFFVFLTYTVTPPVFSAL
                     INAFSYECAFITTAVGAATSGLVLLSSRAKP"
BASE COUNT          711 a         1059 c         1069 g          591 t
ORIGIN      
        1 ccatcaggaa tactggctcg cgatcgtcgc ctacatggtg ctgatggtcg gcaatctcgt
       61 ccgcggtctt taactgcgct ggcggtttgg gcctagagcg agctccagat ggcccagacc
      121 cccgacagaa acgagatggc gagcactgcc gcccggaacc gagcctcgct gaggcggtgg
      181 gccagtctat tccctagcag cgtcgcgata atcacggccg gcgtcaatgc accggcggcg
      241 gcccacgttt cagctgacat tcccgtccag actgcgtgca gcagcgtcac catgacgtaa
      301 cagaacgcga agaacgtcag cgacagcgca cggctttcca gctttccgag acggcgtgcg
      361 gcaacgtaca ggatcaacgg cgggcccggc atcaccagga cagaggtcag cgcgccggcc
      421 gacgccccca ccgttgctgc tgcggcagga tgcggcgggc cgcgaacgac gaagaggtgg
      481 ctgagccagc ttctctcgcc agcgatcagc agggccgtga acaccaacat cacgactccg
      541 actgtcagtt tcaaagtatc gagatccagg gcgaggaaaa cgaagaggcc cgcgggaaaa
      601 cccactaggc tgcccacgag cataggagaa agcagctctt tcggcgcctc ccgccaaagg
      661 ccaggcacca gcatggcact ctgcaccacg tggatcgcca cgagcacctg catggccgaa
      721 ccggattgca tggccgtgag aaagatcggc gcggtgatga gcgcgaagcc gaaaccaacc
      781 gccgattgca cgaacgcgcc agcaaatgcc gcgcccatca agattaccag ggtggagagc
      841 tccggcaccc ctcaggcccg cccagaaaag acgaaagcag cgccgagagc gatcagcgca
      901 aaccccgccg catgtcgcca cgtcagtggc tcacccaggt aaagcacgga aaagccagcg
      961 aaaatggcga gggtgatgac ctcctgcatg gtcttcagct cggccgccga atagacggaa
     1021 tgaccgatcc gatttgccgg caccgcaaga cagtactcca cgaacgcgat cgaccaggag
     1081 cccagaatga cgaccagcaa tggcgcattc ttgaacttga gatgaccata ccaggcgaag
     1141 gtcatgaaga cattcgaggc gacgagcaga agcagcggcg caaggtaagg ccacgacggg
     1201 aagccgacgg cgaacatggg agggtgtgtg ggagagcaag gtcctgagat gaaggtgact
     1261 aggcattagg gtgcagaagt catcttgtct agtggtctag ccgggtggcc cttgcgcccg
     1321 tgtcgccaca acggcagcat gtctgtcacg ccgcgctggc ggccgaaagt gggaccccgc
     1381 gccatgcgcc gcctcaaagc cgatctcctc cttcttcttg ccgcggccat ttggggcctc
     1441 gcgttctact ttcagaagac ggcgatggag cacatcggcc cttttctgtt catcgccgcc
     1501 cgcggagtga ttgcagcggt ttgcctcctg ccgctggcgt tctttgaggc cgccagggta
     1561 acaattccct ggtccgtcgg cttcgtgcag gttgggctgg caggcggcct cgccttcctt
     1621 gtcgctgcgg ccctgcagca gactggaatc atcgactcga ccgtcacgaa tgccggtttc
     1681 ctgaccggcc tgtatgtcgt tgctacgccg ctgctcagtt gggttttgtt cggcgaacgg
     1741 ccagccgccc acgtctgggt ggccgtgcca ttggcctttc ttggagtctg gctgctcggc
     1801 ggaggcacta tcggcggatt gtcgaaagga gatgccctcc ttgccgccgc cgccctcgtc
     1861 tgggcgctgc atatcatcat aaccagccgc gcctcggttt atggccgccc ggtcgcctac
     1921 accgccatgc aatttgcttt cgttgccttt ggcggaggtg tcctggcgct ggcgatcgag
     1981 agcaatacgc tcgaggggtt ttacaaggct ttgccagcga tcacctacgt tggtgtcctg
     2041 tccagcgcct tgaccttcac gttactggcc atcgctttga aggacacacc tccgaccgaa
     2101 gcatcgatcc tgatgagcac ggaaacgctg tttgccgcgg ctgcgggtgt gctgctcgcc
     2161 aacgactggc tgccacctat tggttggatc ggcgccgcgc tgatgttcgc cgccaccgtt
     2221 ctggtccaga tcgggcccgc catggagggg cctaagaagt ccgtttgaat acggcctatc
     2281 gcctgttatt ctgcaggtat ggggtgacgt tggacagccc ttggcgtgcc tggatcgcgg
     2341 ctggcgcgat gctcgctgtg acaacaaaca ttgcgcaggc ggatccgagc cgactcacga
     2401 cgtcggatcg aagaatcaca ccgggacagg tgatcgacgg gtggggtaag ccgatcccct
     2461 gtcgctgccg ttatggtggc cggacctatc agctcggcga gaccgtgtgc atgaaaactc
     2521 aattcggcac gcgactggcc cgctgcgaat tggtcctgaa caatacctct tggatgccca
     2581 cccaagtttc gtgtgtcatt tcttcaatgt gagcgatcag ccgggtcgcg tccaaaagct
     2641 cgtctccatg ctgcgatctg ttcagagctg gccgaatctc acggcttggc cctgctcgac
     2701 aataatacga ggccggaggt ggcggccccg acagccgtcg tgatgaacgc gcattcgtag
     2761 ctgaatgcat tgatcaaggc gctgaacacc ggcggcgtta cagtgtacgt aaggaatacg
     2821 aagaaggtcg ttccggccgt ggcttcgcca accgaatgcg caggcgcgag gttcgcaacc
     2881 tctgcaagat agagtccatt ccaggtcgct accgaggcgc cggcgagccc cgccacggtg
     2941 aacagcagaa gcgggcaatg gtctgaccct agcgcagcga gaagcaaagc agagcctgct
     3001 gagcagcccg ccagcaccat gagtacgggc cgtggggagc caatacggtc cgccaggtag
     3061 ccgaagacga tccggcccac cacgctcgcc gcctgcagca cggcgaacag ggttccagcc
     3121 agagccagcc catagccgag gcccgcatgg agatagatga cgaagaacga aaagcaggca
     3181 ctctggacca tggcgaagcc ggtgcaggcg agcgccagtt gggtcaggcc aggtgcgagc
     3241 gacatggccc gaaacggcct taggaaattc gaaagcgcga agattgcgcc catgcggaag
     3301 ggctgcagtg gccgcgccgt gttgtaatgc cgcggcgcaa gcagcaacgg cacggcgagg
     3361 ccaagacata gaacgcacat caccgaaaaa gcgggccgcc atcccagatg cgtgatggac
     3421 cagcctccca
//
LOCUS       DASUMG010000089         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_253578, whole genome shotgun sequence.
ACCESSION   DASUMG010000089 DASUMG010000000
VERSION     DASUMG010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253578"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFX46_01450"
     CDS             complement(<1..413)
                     /locus_tag="VFX46_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599281.1"
                     /translation="MLSGRCALVTGSVAGLGYAIAESLAKAGAHIVLNGLCDPSEGQA
                     AAERLSSATGVEVQFDGADLRKVSEIERMMAEAADRFGKVDIVVNNAVVRHFSPIEAF
                     STEHWETALAVNVSAAFHCTRLAVPAMRAQGWGRI"
     gene            complement(505..1314)
                     /locus_tag="VFX46_01455"
     CDS             complement(505..1314)
                     /locus_tag="VFX46_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX5599282.1"
                     /translation="MSKAKSLVQKQFGAHAAGYATSPVHAKGASLQRLIDLVRPEKHW
                     RVLDIATGAGHTALAFAPHVAEVIASDITDEMLAEAENLAKARSLTNVTFAKADAMAL
                     PYADGTFDLVTCRIAPHHFPDVPKFVSEARRVLKPGGTFALVDNISPDEESTPGFTPD
                     ELRAAADSHNAFEKLRDPSHGRCLTIGEWRTVVAHAGFTIEHVEQLPKEIEFAPWAER
                     MGADAETTKQLEAMLTGSASALRAFLRPRREGEKLYFSLDEVIVIARKPTD"
     gene            complement(1378..>2301)
                     /locus_tag="VFX46_01460"
     CDS             complement(1378..>2301)
                     /locus_tag="VFX46_01460"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEX5599283.1"
                     /translation="LEPFRTMAVTVEGSLAPGVTAKDVILAVIAQIGTGGGQGYVIEY
                     AGEAIRSLSMEGRMTVCNMSIEAGARAGMIAPDEKTFEYLKGRPKAPKGAAWDMAMRY
                     WETLYSDDGAHFDREVRLDASNLPPLVTWGTSPEDVVSVTGVVPDPDKVEDEAKRAAM
                     KRALDYMGLTPGTKITDIKIDRVFIGSCTNARIEDLREVARIVKGKKVASHVYAMVVP
                     GSGLVKQQAEAEGIDKILKEAGFDWREAGCSMCLGMNPDQLKPRERCASTSNRNFEGR
                     QGRLGRTHLVSPAMAAAAAIAGHFVDVRTWH"
BASE COUNT          418 a          770 c          696 g          417 t
ORIGIN      
        1 atgatccgcc cccagccctg cgctcgcatc gccggcaccg cgagcctagt gcagtggaac
       61 gcagccgaca cgttcacggc aagcgccgtt tcccagtgct cagtcgagaa ggcttctatc
      121 ggcgagaaat gccggaccac cgcattgttg acgacgatat cgacctttcc gaaccggtcc
      181 gcggcctcgg ccatcatgcg ctcgatttcg gagaccttgc gcaggtcggc accatcgaac
      241 tgcacctcaa cgccagttgc gctagaaagc cgctcggcag cagcctggcc ttcgctagga
      301 tcacaaagtc catttagcac gatgtgggca ccagccttcg ccaggctctc ggcaatcgcg
      361 taaccgagac ccgcgaccga cccggtgacc aaagcacatc taccgctcag cataagacct
      421 ccgcaccatt ccggcgtgga cgacaacaga tctccactct cccagaaaga cctaccgcag
      481 gaccaatgta gagcctatgg acttctaatc agtaggtttg cgggcgatga caatgacctc
      541 atcaagcgag aaatagagct tctcgccctc tcgcctgggc ctcaggaacg cccgaagagc
      601 cgaggcggag ccagtgagca tggcctccag ctgcttcgta gtttcagcat ctgctcccat
      661 acgctcagcc catggagcaa attctatttc cttgggcagc tgctcgacgt gctctattgt
      721 gaagcctgcg tgcgcgacca ccgtgcgcca ttcaccaatc gtaagacagc ggccgtggct
      781 cgggtcccgc aatttttcaa aggcgttgtg gctatcggcc gccgctcgaa gctcgtccgg
      841 tgtgaagcca ggcgtcgatt cctcgtccgg agaaatattg tcgacgaggg cgaacgtgcc
      901 cccaggcttc aggacacggc gcgcctctga gacgaatttc ggaacgtcgg ggaagtggtg
      961 gggcgcaatg cgacacgtaa cgaggtcgaa ggtgccatcc gcgtaaggca gcgccatcgc
     1021 atcggccttc gcgaaagtca cgttcgtaag gctgcgggct ttggcaagat tctccgcctc
     1081 cgccagcatc tcatccgtga tgtcgctcgc aatcacctcc gcaacgtgag gcgcaaacgc
     1141 gagcgccgtg tgcccagcac ccgtggcgat gtcgagcacg cgccagtgct tctccggccg
     1201 taccagatcg atcagacgtt ggagactggc gcctttggca tgaacagggg aggtggcgta
     1261 gcctgcggcg tgagcgccga actgcttctg aacgagcgat ttcgcctttg acatccgcca
     1321 cgctccgcct ggatggttgg atacgcaccc gactgacgtc gggcgcgctc aaaagcgtca
     1381 gtgccaagtg cgcacgtcga cgaagtgccc ggcgatggcc gccgctgcgg ccatggccgg
     1441 cgacacgagg tgcgtacggc ccagccggcc ctggcggccc tcgaaattcc ggttggaggt
     1501 cgaagcgcag cgctcgcgcg gcttcagctg gtccgggttc ataccgaggc acatggagca
     1561 gcctgcctcg cgccagtcga agccagcctc cttgaggatc ttgtcgatcc cctcggcctc
     1621 agcctgctgc ttcaccagac ccgaacccgg cacgaccatc gcatagacgt gcgaggccac
     1681 cttcttgcct ttgacgatgc gggccacctc gcgcaggtcc tcgatgcggg cgttcgtgca
     1741 cgagccgatg aacacacggt cgatcttgat gtcggtgatc ttggtccccg gcgtcaggcc
     1801 catgtagtcg agcgcgcgct tcatggccgc ccgctttgct tcgtcctcga ccttgtcggg
     1861 atcgggaaca acgccggtca cggagaccac gtcctcaggg ctcgtacccc aggtgacgag
     1921 cggcggcaga ttcgaggcgt ccagccggac ctcgcggtcg aagtgagcgc cgtcgtcgga
     1981 gtacagcgtt tcccagtacc gcatcgccat gtcccaggca gcgcccttcg gcgccttggg
     2041 ccggcccttc aggtactcga atgtcttctc gtccggcgcg atcatgccgg cgcgggcgcc
     2101 cgcctcgatg gacatgttgc agaccgtcat gcggccttcc atcgacagcg agcggatggc
     2161 ctcgccagcg tactcgatga cgtacccctg cccgccgccg gtgccgatct gggcgatgac
     2221 ggcgaggatc acgtccttgg cggtcactcc cggggcgagg ctgccctcga ccgtgacggc
     2281 catcgtgcgg aacggctcga g
//
LOCUS       DASUMG010000090         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_253590, whole genome shotgun sequence.
ACCESSION   DASUMG010000090 DASUMG010000000
VERSION     DASUMG010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253590"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..505
                     /locus_tag="VFX46_01465"
     CDS             47..505
                     /locus_tag="VFX46_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599284.1"
                     /translation="MVAAVLRCLGGYLVACVAAAIIEVSFVLPPHELLSAGIDRLTAA
                     GIWLLLASLHTAIFAAPFAAIMIGIAERRGLKSSLYYVCVGIGIAALGFLAQLSGTGA
                     VQPVKVTLYPLVAFSVAGIVGGAVYWFLSGRKADQRGWRKRAATRDVATQ"
     gene            577..1212
                     /locus_tag="VFX46_01470"
     CDS             577..1212
                     /locus_tag="VFX46_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599285.1"
                     /translation="MLRFDDYLGVAKFWSDVTLQWMETSAQFFTSILRSFEQSKAVFS
                     PAAGSPWQEANPGSQTPTTSWYKPPACSPFEPDWLGLGWVTQLDASKLTPKEVLGPFN
                     PVEAWTKAMELMVPSMTVGLPVPATTQLRPANWQVAAMPWMIGLGIAMVAPAPTAAAP
                     AEKAQFSAYRSESGHAVAQVTFPNQVVAAVAVPENAVSMLDAFFRWPRIVH"
     gene            complement(1578..2183)
                     /locus_tag="VFX46_01475"
     CDS             complement(1578..2183)
                     /locus_tag="VFX46_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX5599286.1"
                     /translation="MPRLEFWYEFASNYSYLSVMRISDLAQQAGVEVIWKPFLLGPIF
                     KAQGWDTSPFNLYPAKGRYMIRDMQRITASRGLPFVMPPTFPANGLKAARLALIGADE
                     GWIEPVTKAIFEAEFGRGEDIAQPAVLEKALAGLGLDAASLMARAEDPAIKQRLKDQT
                     AEAQSRGIFGAPSFITEDGELFWGDDRLEQALTWASASRKK"
     gene            2344..3579
                     /locus_tag="VFX46_01480"
     CDS             2344..3579
                     /locus_tag="VFX46_01480"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX5599287.1"
                     /translation="MAAKTGGEKIKKVVLAYSGGLDTSIILKWLQETYGCEVVTFTAD
                     LGQGEELEPARRKAEMLGIKPENIFIEDLREEFVRDYVFPMFRANAVYEGVYLLGTSI
                     ARPLIAKKQIEIARKTGADAVCHGATGKGNDQVRFELSYYALEPNIRIIAPWREWSFK
                     GRAELLDFARKHQIPIAKDKEGEAPFSVDANLLHSSSEGKVLEDPAIEPPSIVYQRTI
                     DPEQAPDKPTIITIGFERGDAVSLNGEQLSPATLLARLNDLGRENGIGRVDLVENRFV
                     GMKSRGVYETPGGTILLAAHRAIESITLDRGAAHLKDELMPRYAELIYNGFWFSPERD
                     MLQALIDKSQEHVEGEVRLKLYKGNVIVIGRSSPKSLYNPTLVTFEDDRGAYDQKDAE
                     GFIRLNALRLRTLAMRDRR"
     gene            complement(3621..>4148)
                     /locus_tag="VFX46_01485"
     CDS             complement(3621..>4148)
                     /locus_tag="VFX46_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599288.1"
                     /translation="NDLDLETLDLLQEILSDYEGTVLLVSHDRDFLDRVVTSVIAAEG
                     DGRWVEYAGGYSDMLAQRSGADLPSVQRAASDVGKSKAAEKPAGDRAPAKKRKLSFNE
                     KHALETLPKKIEQLQSEVAALQAKLSDPGFYARDPQGFEKATARLTEAEAELAAAEER
                     WLELELLREELEAES"
BASE COUNT          806 a         1309 c         1234 g          799 t
ORIGIN      
        1 ggcgcgcgta aataacttct caaccgattg cacggggtct tgaaagatgg ttgcagcggt
       61 cctgcgctgc ctcggcggct acctcgtcgc gtgtgtcgct gcggccatta tcgaggtctc
      121 gttcgttctg cctccgcacg agcttttgag cgctgggata gatcgtctga cggcagccgg
      181 catttggctc ctgcttgcca gcctgcacac ggcgatcttt gcagctccgt tcgcagccat
      241 catgattggt attgctgaac ggcgcggcct gaaaagttcg ctttactacg tttgcgtcgg
      301 gatcggcatc gcggcgcttg ggttcctggc acaactctcc ggcaccggag ccgttcaacc
      361 cgtgaaggtc acgctgtatc ctctggtcgc attttccgtc gcgggcatcg ttggtggtgc
      421 cgtgtactgg ttcctctcgg gccggaaggc agatcagaga gggtggcgaa aacgagcagc
      481 cacacgcgat gttgcgacgc aataggattg ttgcggtgcc gaaaaggcgt tatggtgcta
      541 tccggctaag ctgctggcca cgcggaggac atcgcgatgt tgcgttttga cgattacctg
      601 ggggtggcca agttctggag cgacgtaacg ctccaatgga tggaaacgag cgctcagttc
      661 ttcacttcga tcctgcgtag cttcgagcag agtaaggcgg tcttctcacc ggctgcgggc
      721 tcgccatggc aagaggccaa cccgggcagt cagacgccta caacctcctg gtacaagccg
      781 cctgcgtgca gcccgtttga gcccgattgg cttgggctgg gctgggtcac gcagctcgat
      841 gcctcaaagc tgacgcccaa agaagttctc gggccgttca acccggtcga agcttggacg
      901 aaggccatgg agctgatggt cccgtccatg acggttggct tgccggtccc tgcaacaacc
      961 caattgcgcc cagcaaactg gcaagtggcc gccatgccct ggatgatcgg gcttggtatt
     1021 gccatggtcg ctcctgcccc gaccgccgcg gcacccgccg agaaggctca gttctcggct
     1081 tatcggtcgg agagcggcca cgccgtcgcg caggttacct tcccaaacca agtcgtggcc
     1141 gccgtcgcag ttccggagaa cgcagtttcc atgctggacg cgttcttccg ctggcctcga
     1201 atcgtccatt gacgttaccg ggccagcgga cggccgcgaa gttgcaatga gcgccagatt
     1261 ttcagcttac ttggaggcag ggctcgccga agccgacttc tttggcttgg cccggcaata
     1321 ggcttttact tccgtgccgt ctttccgctt cgttgccgaa acccagctgc attcggcctt
     1381 ggccgtgcaa gcctcttgcg tcaggccttt gcactgagat gcctcaggat cgctagccca
     1441 ggctgggaac gtaaacatgc caagagcgat tacgcctgcg gctaagcctt tcgccaacac
     1501 accaatactc actggaatcc ccccgatacg cggaacagag atcatcttag aaacagggga
     1561 tcggctttcc tgcaaggcta ttttttgcgc gaggccgagg cccaagtgag cgcttgttcc
     1621 aggcgatcat cgccccagaa cagctcgcca tcctctgtga taaagctcgg cgcaccgaag
     1681 atgcctcggg actgggcttc cgccgtctga tccttaagac gttgcttgat cgcagggtct
     1741 tcggcgcgcg ccatcagcga tgcagcgtct agacctagtc cggccaacgc tttctcgagc
     1801 accgccggct gtgcaatgtc ctctccccgc ccaaactcgg cttcgaagat cgctttggtg
     1861 accggctcga tccagccttc atcggcgcca atcaaagcga gccgcgctgc cttcagcccg
     1921 ttcgccggga acgtcggagg catgacgaat ggaagaccac ggctcgcggt aatccgctgc
     1981 atgtcgcgga tcatgtagcg tcctttcgcc gggtaaagat tgaatggtga ggtatcccag
     2041 ccctgcgcct tgaaaatcgg ccccagaaga aaaggcttcc agatgacctc gacaccggcc
     2101 tgctgggcca agtctgagat gcgcatgacg gagagatacg agtaattcga ggcgaactcg
     2161 taccagaact ctaaacgggg catgggaaag acctctcgag caacgtagga gctcaaacct
     2221 accacggcca atcggaactg agacaaatag ggcagaccag cctcgtagga ggccccttgg
     2281 ccgctggcgg atcgcgcgcc tatagtgcgc cgccccattc ttacgacatg agcaggacac
     2341 acgatggctg cgaagaccgg cggagagaag atcaagaagg ttgtgcttgc ctattcaggc
     2401 ggcctcgaca cctccatcat cctgaagtgg ctgcaggaaa cctacggctg cgaggtggtg
     2461 acgttcacag ccgatctcgg ccagggcgaa gagcttgaac ccgcgcgtcg caaggcggaa
     2521 atgctcggca tcaagccgga gaacatcttc atcgaggacc tgcgggaaga gttcgttcgc
     2581 gattacgtgt tccccatgtt ccgcgccaac gcggtctatg aaggggttta cctgctcggc
     2641 acctcgattg cgcgcccgct gattgccaag aagcagatcg agattgcgcg caagaccggc
     2701 gcggacgccg tctgccacgg tgccaccggc aagggcaacg accaggtgcg cttcgagctc
     2761 agctactacg cgctggagcc gaacatccgc attatcgccc cctggcgcga gtggagcttc
     2821 aaaggccgcg ccgagctgct ggacttcgcc cgcaagcacc agatccccat tgccaaggac
     2881 aaggaaggcg aggcgccgtt ctctgtcgat gccaatctgc tccactcgtc ttcagaagga
     2941 aaagtgctgg aagatccggc catcgagccg ccgtccatcg tctaccagcg cacgatcgac
     3001 cccgagcagg cgccggacaa gccgaccatc atcaccatcg gcttcgagcg cggcgatgcc
     3061 gtgagcctca acggcgagca gttgtcgccg gccacgctgt tggcgcgcct caacgatctc
     3121 ggccgtgaga acggcatcgg ccgagtcgat ctcgtcgaga accggttcgt cggcatgaaa
     3181 tcgcgcggcg tgtacgagac gccaggcggc accatcctgc tcgctgcgca ccgggccatc
     3241 gagtcgatca cgctcgaccg cggcgccgcg cacctcaagg acgagctgat gccgcgttac
     3301 gccgagctga tctacaacgg cttctggttc agccccgagc gcgacatgct ccaggcgctc
     3361 atcgacaaga gccaggagca cgtcgaaggc gaggtgcggc tgaagctcta caagggcaac
     3421 gtcatcgtca tcggccgctc gagcccgaaa tcactttaca acccgacact cgttactttc
     3481 gaggacgacc gcggcgccta cgaccaaaag gacgccgagg gcttcatccg gctgaatgcg
     3541 ttgcggctcc ggaccctcgc gatgcgcgac cgacgctgac gtcctgcaag aatgttcgga
     3601 ggcggagagc ccgatcccaa ttagctctcc gcctccagct cctcacgcaa cagttccaat
     3661 tccagccagc gctcctcggc ggctgcgagt tcagcctccg cctcagtaag acgggctgta
     3721 gccttctcga aaccctgcgg atcgcgcgcg taaaatccgg gatcggagag tttggcctga
     3781 agagccgcca cctctgactg cagctgctcg atcttcttcg gcaacgtttc cagcgcgtgc
     3841 ttctcgttga acgagagctt gcgcttcttg gccggcgccc ggtcgccagc aggcttttcc
     3901 gctgccttgg actttccaac atctgacgcg gcccgctgca ccgaaggcag gtccgcgcct
     3961 gagcgctgcg caagcatatc ggagtagccg ccagcgtact ccacccagcg cccatcgcct
     4021 tccgccgcga ttaccgaggt gacaacgcga tcgaggaaat cgcggtcatg gcttaccagc
     4081 agaacggtgc cttcgtagtc ggagaggatc tcctgcagga ggtccaaggt ttcaaggtcg
     4141 agatcgtt
//
LOCUS       DASUMG010000091         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1136939, whole genome shotgun sequence.
ACCESSION   DASUMG010000091 DASUMG010000000
VERSION     DASUMG010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136939"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFX46_01490"
     CDS             <1..271
                     /locus_tag="VFX46_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5599289.1"
                     /translation="GRTEFLGMEGGDRWLADRAEALRPEYQARLRAHRAGLEALAARL
                     GWSLLVHHTDRSAAEPLLTLIMRLQNSARDYRWVAPAAANPGAAG"
     gene            268..>2539
                     /locus_tag="VFX46_01495"
     CDS             268..>2539
                     /locus_tag="VFX46_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX5599290.1"
                     /translation="MSLGALAFLNPWLLAGLAALPIIYWLLRTVPPRPRQVAFPATRI
                     LVGLENREKTPAQTPWWLMLIRMMAAALVIFALAEPVLNPERQAALSGSGPVAIVVDN
                     GWASASRWALRTRMVDRVIDEAERQNRAVVLLPTASTSRMPTANLEAPNDARAAAAAL
                     QPQPFAPDRMAAARALETALQGQADASIVWLTDGIDHDGNTAGFLERLSGLGGACLTV
                     IEAGAGEEPLGVATNVGSGGRLEVTVVRTGGGAREGVLHALSARGQRLGEAEFRFAPG
                     QTVAKAHFELPLELRNQVTRIEIAGEPSAGAVHLLDARSQWNRIGLLSGESSEQAQPL
                     LGPLYYIERALAPFAELVKPRDPNLLTGIDTILKQNASIVMLADIGTLTGEVKTRLEE
                     WVRKGGVLVRFAGPRLEKGGDDLLPVGLRAGGRTLGGALSWSTPQPLAPFPEDSLFAG
                     LTVPNDVTVSRQVLADPTQLGPDVKVWARLADGTPLVTASALGDGQLVLFHVTANSDW
                     SNLPLSGLFVEMLRRISTLGRLGGTGEATAADTSGANAPVAETSVLTPVQLLDGFGVL
                     KSPPPTAQAIPVADLPNLTPSAEHPPGYYGAQGAPRAFNLITPDTVLRPLPGLPASAQ
                     RRAYEGQSMTPLKPWLLATALGLLFADILAVVLLQMGGLALRRPVQRAARAAGAVALG
                     VMLAHALPNQAEAQLTPGVREPVTMSAKDWLAVKATSKVTFGYVLTGDPETDEISRAG
                     LLGLGKVLTLRTAVEPG"
BASE COUNT          404 a          814 c          871 g          450 t
ORIGIN      
        1 ggggcgcacc gagtttcttg gaatggaggg cggcgatcgc tggctcgcgg accgcgccga
       61 ggcgctgcgg cccgagtatc aggcgaggct gagggcccac cgggccggtc tcgaagccct
      121 agccgctcgc ctcggatggt cgctccttgt ccaccacacc gatcgctcgg cggctgagcc
      181 tttgctgacc ctcatcatgc ggttgcagaa tagcgcccgc gattaccgct gggttgcacc
      241 agctgccgcg aacccaggag cagccggatg agccttggtg cgctcgcctt cttgaatcct
      301 tggttgctcg cggggctggc ggccctgccg atcatctatt ggctgctgcg gactgttccg
      361 ccacgcccgc ggcaggtcgc cttcccggcc acgcgcatcc tggtcggcct cgaaaaccgg
      421 gagaagacgc cagcgcaaac accctggtgg ctgatgctca ttcggatgat ggcagcagcg
      481 ctggtgattt tcgctctggc tgagccggtc ctgaaccctg aaaggcaggc tgctctgtcg
      541 gggagcggac ccgtcgccat cgtcgtcgac aacgggtggg catcggcatc gcggtgggcg
      601 ttgcggaccc gcatggtcga tcgggtgatc gacgaggccg agcgccagaa ccgagcggtc
      661 gtgctactcc ctacggcttc taccagccgt atgccgaccg ccaacttgga agcgccgaat
      721 gatgctcggg cagcagccgc cgcactgcag ccacaaccgt tcgcgccaga ccgaatggcc
      781 gccgcccgcg cgctggagac ggcccttcaa ggtcaggccg acgccagcat cgtctggctg
      841 acggatggca tagatcacga cggaaacacc gccggatttc tggaacgact ctctggtctt
      901 ggaggagctt gcctgacggt gatcgaagct ggcgccggag aggagccgtt gggtgtggcg
      961 accaacgtcg gcagcggcgg ccggcttgaa gtcaccgtgg tgcggactgg cggcggcgct
     1021 cgcgaaggtg tgctgcatgc gctctcggcg cggggccagc ggctaggtga ggccgagttc
     1081 cgcttcgcgc caggacagac ggtcgccaag gctcattttg agctgccgct cgagctgcgc
     1141 aaccaagtga cccggatcga aatcgccgga gagccctccg cgggggccgt tcacctcctc
     1201 gacgcgcgct cgcaatggaa ccggatcggc ctgctttcgg gagagagcag tgagcaggct
     1261 cagcccctgc tcgggccact ttactatatt gaacgcgcgc ttgccccgtt tgcagagctc
     1321 gtcaagccgc gagatccaaa ccttctcacc ggcatcgaca cgatcctgaa acagaacgcc
     1381 tcgattgtca tgctggcgga catcggtacg ctgacgggcg aggtgaagac ccggctggag
     1441 gagtgggtcc ggaaaggcgg cgttctggtg cgtttcgccg gcccccggct cgagaagggc
     1501 ggggatgatc tgcttcccgt cggattgcga gcgggtggcc ggacccttgg tggtgccctg
     1561 tcgtggtcca cgccccagcc actggcgccg ttccccgaag acagcctttt tgcagggctg
     1621 acggtgccca atgatgtcac cgtgagccgg caggtccttg ccgatccgac gcagctcggg
     1681 cccgacgtga aggtctgggc gagattggcg gacggcacac ctcttgtgac cgcgagtgcc
     1741 ctgggcgacg gccagctcgt gctattccac gttactgcca actcggactg gtccaacctt
     1801 ccgttgtcgg ggctgttcgt cgaaatgctc cgccgcatca gcacgctcgg ccggcttggt
     1861 ggcactggtg aagcgacagc agccgacacc agtggcgcca acgcaccagt cgcggagacc
     1921 agtgtgctga cgcccgtgca gctcctcgac ggcttcggcg tcttgaagtc gcctccgccg
     1981 acggcccagg cgataccggt ggccgacctg cccaatttga cgcctagtgc tgaacatccg
     2041 ccgggatact atggtgcgca gggcgctccg cgtgccttta acctgatcac gcccgatacg
     2101 gtgctgaggc cgctgccggg attgccggcc tcggcccaaa ggcgtgcgta cgaaggtcag
     2161 tcgatgacac cgctgaagcc ttggctgctg gcgactgcgc tgggtctgct gttcgcagac
     2221 atcctggcag tcgtcctgtt gcagatgggc ggccttgctc tgaggcgccc cgtgcagcga
     2281 gctgctcgtg cggcgggagc cgttgcgctc ggcgtgatgt tggctcacgc cctgcctaat
     2341 caggccgagg cgcaattgac ccctggggtg cgtgagccgg tcacgatgag cgccaaggac
     2401 tggttggcgg tgaaggccac gagcaaggtg accttcggct atgtcctaac cggagatccg
     2461 gagacggatg aaatcagccg cgccggcttg ctgggcctag ggaaggtgtt gacgctgcgg
     2521 acggcggtcg agcccggcg
//
LOCUS       DASUMG010000092         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1137056, whole genome shotgun sequence.
ACCESSION   DASUMG010000092 DASUMG010000000
VERSION     DASUMG010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137056"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..676
                     /locus_tag="VFX46_01500"
     CDS             203..676
                     /locus_tag="VFX46_01500"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003534138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEX5599291.1"
                     /translation="MAAMRLALYQPDIPQNTGTIMRLAACMNVPVDVIGPPGFDLSDR
                     ALKRAALDYLPYVDLTRHVSWEEFLEKRASTEGIGRLVLLSARGDHPYTEFRFARDDT
                     LLLGRETAGVPDSVRAVADASVRIPMRSDLRSLNIAVAAAMVLGEALRQTGLFPS"
     gene            complement(685..1404)
                     /locus_tag="VFX46_01505"
     CDS             complement(685..1404)
                     /locus_tag="VFX46_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEX5599292.1"
                     /translation="MGDISPIKALVFDVFGTVVDWRTSIIQELEAFGAQKGISADWAA
                     FTDDWRALYQPSMEEVRAGRRPWTILDVLHRESLEKLLTKYGIDGLSEEEKDHINKVW
                     HRLKPWPDSVPGLHRLKARYLIGTLSNGNIGLLAWMAKNAGLPWDVILGAEPARAYKP
                     LPEAYLRTAKILGLRPEQMMLVAAHNDDLAAAAAQGYATAFVARPTEYGPHQTKDLKA
                     EREWDGVTDSFLGLAEALGCP"
     gene            complement(1406..>2021)
                     /gene="aroC"
                     /locus_tag="VFX46_01510"
     CDS             complement(1406..>2021)
                     /gene="aroC"
                     /locus_tag="VFX46_01510"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5599293.1"
                     /translation="IGPHAVDRSRWNWDEVESNPFFCPDHDSVQTWASYLDQIRKSGS
                     SVGAVIEIVAEGVPPGLGAPIYGKLDQDLAAALMSINAVKGVEIGAGMAAAELTGEEN
                     ADEMRIGPDGRPVFLSNNAGGILGGISTGQPIVCRFAVKPTSSILTPRKTIDRFGNET
                     EIMTKGRHDPCVGIRAVPVGEAMVAIVLADHALRHRAQTGKWEF"
BASE COUNT          375 a          609 c          631 g          406 t
ORIGIN      
        1 gagacacgtc gggcgtgaaa agagacggta tcgaccagtc gcaacctata tgttggcaaa
       61 tcccccgctc agaacggcgc tttttgccac gccgaccttt tgacatcgtt tgtccatgct
      121 ctcaaggcgg ggaagcagaa cggcaaagag gcaatttggc gcatacgctt gcgaatgcgc
      181 cttggctgca taatgaggcg gaattgctgc gatgagactc gccctctatc agccggatat
      241 cccgcaaaat accgggacga tcatgcgtct tgcggcgtgc atgaacgtgc ccgtcgatgt
      301 cataggtcca ccgggatttg atctgtcaga ccgtgcgttg aagcgcgcgg cgctcgacta
      361 cctgccgtat gtcgatctca cccggcacgt gagctgggag gagtttctgg aaaagcgcgc
      421 gagtaccgag ggcatcggac ggctcgttct cctcagcgcg cgcggcgatc atccctacac
      481 agagtttcgc ttcgcgcgcg atgatacctt gttgctgggc cgcgaaaccg ccggcgtgcc
      541 tgacagtgtc cgggctgtgg ccgatgcaag cgtgcgcata ccgatgcgca gcgacctgcg
      601 ctcgctcaac attgcggttg ctgccgcgat ggtgttgggt gaagcgctgc ggcaaacggg
      661 gctgtttccg tcctagaggc gcgattaggg acagccaaga gcctctgcga gaccgaggaa
      721 gctgtcagtg acaccatccc attcccgctc ggccttcaga tcctttgtct gatgaggccc
      781 atactccgtg ggccgcgcga cgaatgccgt cgcatatccc tgggcggcag ctgcggcgag
      841 gtcgtcattg tgcgccgcaa cgagcatcat ctgctcaggc ctgaggccaa ggatctttgc
      901 ggttcgcagg taggcttcgg gaagcggttt gtaggcgcgc gccggctccg cgccgagaat
      961 gacgtcccat ggcaggccgg cgttcttcgc catccatgcg agcaagccga tgtttccatt
     1021 ggacagtgtg ccgatgaggt agcgggcctt gagccgatgc aaccccggca cgctgtcagg
     1081 ccacggtttg agccggtgcc agaccttgtt gatgtggtcc ttctcttctt cgcttagtcc
     1141 gtcaatgccg tatttggtga gtagcttctc caggctctcg cggtgcagca catcaagaat
     1201 ggtccagggg cggcggcctg cgcgtacctc ctccatcgag ggctgataga gggcgcgcca
     1261 gtcgtccgtg aacgccgccc aatcggcgga gatacctttt tgcgcgccga aggcctcaag
     1321 ctcctggatg atgctcgtgc gccagtcgac aacggtgccg aagacgtcga agacgagtgc
     1381 cttgattggg gagatatcac ccatttcaaa actcccactt gcccgtttgt gcgcggtggc
     1441 gcaacgcgtg gtcagccagg acaatcgcga ccatggcctc gccgacagga acggcgcgga
     1501 tgccgacaca cggatcgtgc cggccctttg tcatgatctc ggtttcattg ccgaaccggt
     1561 cgatggtctt ccgcggcgtg aggatggatg aggtcggctt cacggcgaag cgacagacta
     1621 tgggctgacc cgtcgagatg cccccgagaa tgccgcctgc attattcgac agaaaaaccg
     1681 ggcgtccatc cgggccaatg cgcatttcgt cggcgttttc ctcgcccgtc agctcggccg
     1741 ctgccatgcc cgcgccgatc tcgacgcctt tcacggcatt gatgctcatc agcgctgcag
     1801 ccaggtcctg atcgagcttg ccgtagatcg gagcaccaag ccccggcggg acgccttcag
     1861 ctacgatttc gatgacggcg ccgacggagg aaccggattt gcgaatctga tcgagataag
     1921 aagcccacgt ctgaacagag tcgtgatccg ggcagaagaa cgggttgctc tcaacctcgt
     1981 cccagttcca gcgcgagcgg tcaacagcgt gcgggccgat g
//
LOCUS       DASUMG010000093         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_334056, whole genome shotgun sequence.
ACCESSION   DASUMG010000093 DASUMG010000000
VERSION     DASUMG010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334056"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..456
                     /locus_tag="VFX46_01515"
     CDS             16..456
                     /locus_tag="VFX46_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX5599294.1"
                     /translation="MVKRAASYPEVDRIFVHPAIKKALCEGASKVGSDRSWLRKVRPY
                     WGHHYHFHIRLRCPSDSASCQNQPAPPPGDGCGKELNDWLALIAAPPKPAEPSKPKPP
                     LTLAELPKQCRTVLEASPPSEISAEAPLKVPVPVRAVRGQSAQQ"
     gene            617..1687
                     /locus_tag="VFX46_01520"
     CDS             617..1687
                     /locus_tag="VFX46_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599295.1"
                     /translation="MVDDRKEGGEVSAPSPSSRSEAATTADIQPNDAAPQEPVTAPAA
                     ASTSVVAPESKPTETAAAVPPATDKDKAEGSGETPKSSIQPQTAPTGGVGRRVYQASA
                     VRKTLMSFVFLILLPFFASLPAMMVQRIMHQLWPDMLGFTIFAAAFTAVMLLVVFELL
                     RAIRARVVIGESAVQLTLPAGRGIVMPKLLYRTHTIPYSDIEAVETRRELYGGVIAPV
                     MLRGARIITKSGKTVPLGYVNEANTDPALPVPEIAAEIARRAGLEVIDRGTVRRQVHK
                     KLRGIRANDSDAAPITEADIEALNRRHNKVMVAICSALFLIVAGGLLVDISKSELDLG
                     EQARQLVQRIIPSSDVQRAGAQ"
     gene            complement(1709..2023)
                     /locus_tag="VFX46_01525"
     CDS             complement(1709..2023)
                     /locus_tag="VFX46_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599296.1"
                     /translation="MLILRVLSGVFLLAATIALISEATRAQLGVPGAPFTPLLTQLAE
                     STPVLLSSLEKMVRNVHAYLWDPLLLSILQLPAWVSLGSIGLVLGWLGRRRPQSIRVF
                     TN"
     gene            complement(2123..2551)
                     /locus_tag="VFX46_01530"
     CDS             complement(2123..2551)
                     /locus_tag="VFX46_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX5599297.1"
                     /translation="MALTQASTTITVSTRGPGFTDLTHEVQGWLHKIGARDGLLTAYI
                     RHTSASLTIQENADPDVRRDLLDALDRLAPRSREYRHQLEGSDDMPAHIKTALTSVSV
                     SIPVVNGRAVFGTWQALYLIEHRDAPHRREVVLHFLGETA"
     gene            complement(2576..3010)
                     /locus_tag="VFX46_01535"
     CDS             complement(2576..3010)
                     /locus_tag="VFX46_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599298.1"
                     /translation="MRTLPIAVLLIAIVALGVSGVASQEKQGRFVMSPTENGFVRLDT
                     ETGAMSLCAQRNGRWACELMEDEAQKLHDEIARLRDEVKRLEEQLAAAEPRKRLELPT
                     EEEVDRALDYFENIFRKFRERIERFEDQERKERPEQKDGSQL"
BASE COUNT          766 a         1112 c         1112 g          689 t
ORIGIN      
        1 gatgcccacg tccgtttggt aaagcgggcg gcgtcctacc ctgaggtgga ccgtattttc
       61 gtgcatccag cgataaaaaa ggcgctctgt gagggcgcca gcaaggttgg ctcggaccgg
      121 agctggttgc gtaaagtccg cccctattgg ggacatcact atcattttca catccgattg
      181 cgatgcccat ctgatagcgc atcgtgccag aaccaacctg cgccccctcc gggtgatggc
      241 tgcgggaaag agctgaatga ctggctggct ctgatcgcgg caccgccgaa accggccgag
      301 ccgtccaagc cgaaaccgcc cctgacatta gctgagcttc cgaagcagtg ccgtacggtg
      361 ctggaggcaa gccctccaag cgaaatctcg gccgaagcgc cgttgaaagt gccagtcccg
      421 gtccgggccg tgagagggca gtcggcacag cagtaggacg cgaacggaaa caaacttgcg
      481 tgcgcacaga agtgtgccct tcgagaatgc gcacgcgtgc ggggaaggat atagtaatct
      541 ccaggcggca gcggctcagc acgcaagccg attatgccag gctttgcttg tgtccggcgt
      601 ctaatgggag ggggtcatgg tcgacgatcg gaaagagggt ggagaagtat cggctccctc
      661 tccaagctcc agaagcgaag ccgccaccac tgccgatata caaccgaacg atgctgcccc
      721 acaggagccg gttaccgcgc ccgcggctgc ctccaccagc gtggtagctc ccgaatctaa
      781 gccaactgag acagctgcag ctgtccctcc tgcaactgat aaggataagg ccgaaggctc
      841 cggcgagaca ccgaagagca gcatacagcc gcagaccgct cccacaggtg gggtcggccg
      901 ccgcgtctac caagccagcg ccgtgcgcaa gacgctgatg tcgtttgtct tcctcattct
      961 gctgccgttt tttgcgagcc tgccagcgat gatggtgcag cggatcatgc atcagctctg
     1021 gccggatatg ctgggcttca ccattttcgc cgcggccttc actgcggtca tgctgctcgt
     1081 ggttttcgag ctgctccgcg caatccgggc gcgcgtcgtg atcggggaaa gcgcagtcca
     1141 gctcaccctg ccggcaggtc gcgggatcgt gatgccaaag ctactctacc ggactcacac
     1201 catcccgtat tcggacatcg aagctgtaga aacgcggcgc gagctttacg gcggggtcat
     1261 cgccccggtc atgctgcggg gagcacggat catcacgaaa agtgggaaga cagttccgct
     1321 cggatacgtg aacgaggcga acacggaccc ggcgttgccg gtgccggaga tcgccgccga
     1381 aattgcgcgc agggccggcc ttgaagtcat cgaccgtggc acggtccgcc ggcaagttca
     1441 caagaagctt cgcggcattc gagcaaacga cagcgacgca gctcctatta cggaggccga
     1501 catcgaagcg ctcaaccggc gccataacaa ggtcatggtg gctatctgca gcgcgctctt
     1561 cctgatcgtg gccggagggc tgctggtcga catttcgaag tcggagctcg atctcggtga
     1621 gcaagctcgg cagctcgttc agcggatcat cccatcctcg gacgtgcaac gtgctggagc
     1681 gcaatagccg taatactggg atcagcgcct agttggtgaa gactctgatg ctctgagggc
     1741 gacggcgacc cagccacccg agcaccaaac cgatgctacc caggctgacc caggccggca
     1801 gctgcagaat ggataagagc agcgggtccc acaggtacgc atgaacattg cgaaccattt
     1861 tttccagcga gctcaacagc accggcgtgg actcggcgag ctgggtcaat aagggtgtaa
     1921 acggagctcc cgggacgcca agctgcgcgc gcgttgcctc cgaaatcagg gcaatggtcg
     1981 ctgccaatag gaacacaccg ctaagaacgc gaagaatcag catcggctat ggatcgggtt
     2041 ggtccattga aagtgcgaag gtccgacggc cgttgtagca agcccaattc tccaaagagt
     2101 agagagcggc tgcggttggc attcaagcag tctctcccag aaaatgcagg accacctcac
     2161 gccgatgagg ggcatccctg tgctcgatga gataaagcgc ttgccacgtg ccgaagactg
     2221 ctctcccgtt caccaccgga atgctcactg acacggaggt cagcgctgtc tttatatgcg
     2281 ccggcatatc gtccgagcct tccaattggt gccggtactc ccgcgagcga ggtgccagcc
     2341 ggtccagcgc atcgagaagg tcacggcgca catctggatc ggcgttctcc tgaatggtca
     2401 gcgatgctga cgtgtggcga atgtacgctg tcagcaagcc gtctcgtgcc ccgatcttgt
     2461 gcagccaccc ctgcacttca tgcgtgagat ccgtgaagcc cggacctcgg gtagagacag
     2521 tgatcgtcgt gctcgcctgc gtcagcgcca tggagtttga acgcgcgtcg ttcggtcaaa
     2581 gctgagagcc gtccttctgc tccggccgct cctttcgctc ctggtcttcg aaacgttcaa
     2641 tcctttcgcg gaacttgcgg aagatgttct cgaaataatc cagggctctg tcgacctcct
     2701 cttcggtggg cagctcgagc cgctttctgg gctcagcggc agccaactgc tcctcgaggc
     2761 gcttcacctc gtcgcgcagc cgcgcgatct cgtcgtgcag cttctgagcc tcatcctcca
     2821 tcaattcgca ggcccacctg ccgttccgtt gggcgcagag ggacatggcg cccgtctcgg
     2881 tatcaaggcg cacgaacccg ttctccgtcg ggctcatgac gaatcgcccc tgcttctcct
     2941 ggctcgcaac cccgctcacg ccgagggcga cgatggcgat caaaagaacg gcaataggta
     3001 gggtgcgcat tcgcgtctcc ctttcactga tttggatggg ctgtccgatg aggaagccga
     3061 tgctgactcg ttgatgtcga ctcgggttgc gcccgtttca agctggctgg aaagaagacg
     3121 caccttcggc atcgaagaga gtcccgcatt gagcagagtt atgcggctag tttgcggcaa
     3181 gtgtgttggg aaacactgaa gaattactga aaatggtaac aatcgttaac tgaactttta
     3241 ctgaaacttc atagtgctcg aagcacggct ccaaatcgtg ccgcgtatcg gaccggcgga
     3301 tgcgctcgag cggcacagat gatccgcggg aaatgacttc ccgctctctc cagccttgac
     3361 cgagggcatt cgcaacgaag ctcgacctgg cgggggggag ggatgacata caacaagcgg
     3421 agctctgctg acgaggccta ccactcggca aagacaacgc ctccatctga tcgcgaacgc
     3481 agcgcggatg agctgagggg ccttctgcaa cgcatcgccg accatattgc cgcagccgat
     3541 cagcgccagc aggcgacttc aggggacgct cgaactcgat tggaccgcct ggccgagaat
     3601 tggcctggta gagcggctcc agcagcaaca caggatggag aattctggtc agaccctctg
     3661 caagaggagc tgcgccgcc
//
LOCUS       DASUMG010000094         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_655775, whole genome shotgun sequence.
ACCESSION   DASUMG010000094 DASUMG010000000
VERSION     DASUMG010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655775"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VFX46_01540"
     CDS             complement(<1..479)
                     /locus_tag="VFX46_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5599299.1"
                     /translation="MTISDEREAQVVAWLAEQKDAMIDLLRDVVNIDSGTYDKAGVDA
                     VGARFERFFSEHGLVTSREANERFGDAIHIRLDDRLTNEKPVLLLGHRDTVFSKGEAV
                     RRPFRIENGRAYGPGVADMKAGLVMNAFVLAAFAKFGSPVPVAGLITSDEEIGSPACR
                     "
     gene            complement(712..1845)
                     /gene="dnaJ"
                     /locus_tag="VFX46_01545"
     CDS             complement(712..1845)
                     /gene="dnaJ"
                     /locus_tag="VFX46_01545"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX5599300.1"
                     /translation="MAKRDYYEVLGVSRNATEQELKAAFRRLAKECHPDFNPGDKDAE
                     RRFKEIAEAYETLRDPQKRAAYDQFGHAAFDGSARGPAGGFGPDFAASMSDIFDDLFG
                     EFMGGRRRNRSGRERGADLRYNLEITLKEAYTGKTAQIRVPSSVSCDKCSGTGAKPGT
                     RPTSCPTCGGYGKVRASQGFFTIERTCPSCHGRGEVIEDPCPNCSGSGRVMRERTLSV
                     NIPAGVEDGTRIRLAGEGEAGLRGGPPGDLYIFLSIKPHEFFQRDGADIFCVVPISMV
                     TAALGGQIEVPTVDGSVTRVKIPEGTESGKQFRLKGKGMPVLRSKTYGDMYIQVRVET
                     PKNLTKRQRELLEEFERESHKDTSPESAGFFSRVKEFFEGRGA"
     gene            complement(1970..>3062)
                     /locus_tag="VFX46_01550"
     CDS             complement(1970..>3062)
                     /locus_tag="VFX46_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX5599301.1"
                     /translation="NLPFITADQSGPKHLTMKLTRAKLESLVEDLIAKTRGPCEQALK
                     DAGLKAAEIDEVVLVGGMTRMPRVQQVVKELFGKEPHKGVNPDEVVAVGAAIQAGVLQ
                     GEVKDVLLLDVTPLSLGIETLGGVFTRLIERNTTIPTKKSQIFSTAEDGQTAVTIKVY
                     QGEREMAADNKLLGTFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSARDKATGKEQSI
                     RIQASGGLSDEDIQRMIKEAEQHAAEDKRRRELVEAKNHLEALIHSTERSLSESGDKL
                     PAGVKSEVEAAISSAREAAQSNDIERVKSATQSLAQAALKIGEAVYSGQPGAEAGGAE
                     AKSKAESGSENVVDAEFEEVKDNNKKDSA"
BASE COUNT          501 a         1000 c          947 g          614 t
ORIGIN      
        1 cggcatgccg gtgagccgat ttcctcgtcg ctggttatga gaccggcgac gggcaccggg
       61 ctaccaaact tggcgaaggc cgcgagcacg aatgcgttca tgacgaggcc ggccttcatg
      121 tccgcaacac ctggcccgta ggcgcgaccg ttttcgatcc ggaaaggtcg tctgacagct
      181 tcacccttcg agaacactgt atcgcggtga cccagcaaaa ggaccggctt ttcattcgtt
      241 aggcgatcat cgaggcgaat gtggatcgca tccccgaagc gttcattggc ctcgcggctt
      301 gtgacgagcc catgctccga gaagaaacgc tcgaaccgcg cgccaaccgc atccaccccc
      361 gccttatcgt aggtgcctga gtcgatgttc acgacatcgc gcaacaggtc gatcatcgcg
      421 tccttctgct cggccagcca cgctacgacc tgtgcctcgc gctcgtcgga gatcgtcatt
      481 gggtgcccct gggttagact gtgcgggggc cattgtagag catccggccc gggacaggcg
      541 atggcctgct tggtcttgat cgccgtttgg cagcgggagg aaagcgtcgt cccgggcctt
      601 gtgcccggga tccttctatc cgcgaacgcg gagcctgggg tgaagtggat cccggcgaca
      661 agcgccggga tgacaagtgg gtggtcgaaa aacctaagga ggggcggagg ctcaggcccc
      721 gcggccttca aagaactctt tcacgcggga gaagaagcca gcgctttcag ggctcgtatc
      781 cttgtggctt tcgcgttcga actcctcgag caactcgcgc tggcgcttgg tcaagttctt
      841 cggcgtctca acccggacct gaatatacat gtcgccgtaa gtcttcgagc ggagcacggg
      901 catgcctttg cccttgagac ggaactgctt cccgctctcc gtcccttccg ggatcttcac
      961 acgtgtgacg ctgccatcga ccgtcggcac ctcgatctgg ccgcctagtg cggcggtgac
     1021 catcgagatt ggaaccacgc agaaaatgtc ggcaccgtca cgctggaaga actcgtgcgg
     1081 cttgatcgag agaaagatgt aaaggtcgcc cggcggcccg ccgcggaggc cggcctcgcc
     1141 ctcgccggcg agccggatgc gggtgccgtc ttcgacaccg gctggtatgt tcacggacag
     1201 cgtacgctcg cgcatcacgc ggcccgagcc ggagcagttt gggcacggat cctcgatgac
     1261 ctcaccgcgg ccgtgacagc tcggacaggt ccgttcgatg gtgaagaagc cctgtgaggc
     1321 gcgcaccttg ccgtagccgc cacaagtcgg gcagcttgtc ggcctggtgc cgggctttgc
     1381 tccggttccg gagcacttat cgcaggaaac gctggagggc acacggattt gtgccgtctt
     1441 ccccgtgtag gcctccttta gcgtgatttc gagattgtaa cgtagatcag cgccccgttc
     1501 gcgcccggac cggttccggc gccgcccgcc catgaactcg ccgaacaggt cgtcgaagat
     1561 gtcggacatg gaggcggcga agtccggccc gaagccaccg gccggccctc gagcactacc
     1621 gtcgaaggcc gcatggccga attggtcgta ggcggcgcgc ttctgcggat ctctaagagt
     1681 ttcgtaagcc tcagcgattt ccttgaagcg ccgctcggcg tccttgtccc ccgggttgaa
     1741 gtcgggatgg cattccttgg ccagtcgccg gaaggcagcc ttcagctctt gttcagtcgc
     1801 gtttcgggat acgccaagaa cctcgtaata gtcgcgcttg gccatttatg gacgcccgcc
     1861 attccttccc gccttcgagg caccagcctg ctccgccatt acggacaaga ccctcgccga
     1921 aatcggggat tacggcgagg gccctttggc tgctgtcccg ttcggccggt taggccgaat
     1981 ccttcttgtt attgtccttt acttcctcga actcggcatc aaccacattc tcggaaccgg
     2041 actcggcttt cgacttggcc tccgctccgc cggcctcagc gccaggctga ccgctgtaga
     2101 cggcctcgcc gatcttgagc gccgcctgcg ccagcgattg cgtagccgac ttgacgcgct
     2161 cgatgtcgtt gctctgagca gcctcacgcg cggacgagat ggccgcttcg acctccgact
     2221 tgacgccagc cggaagcttg tcgcccgact ccgagagtga ccgctcggtc gagtggatga
     2281 gcgcctcgag gtggttcttg gcctcgacca gctcccgccg cctcttgtcc tcggcagcgt
     2341 gctgctcggc ctccttgatc attcgctgga tgtcctcatc gctgaggcca cccgaagcct
     2401 ggatgcggat cgactgctcc ttgccggtcg ccttgtctct cgcggagacg ttgacgatgc
     2461 cgttggcgtc gatgtcgaac gtcacctcga tctgcggcac gccgcgaggt gccggcggga
     2521 tgccgaccag gtcgaaagtg ccaaggagct tgttgtcggc cgccatctcg cgctcaccct
     2581 gatagacctt gatggtgacc gcggtctggc catcctcggc cgtcgagaag atctggctct
     2641 tcttcgtcgg gatggtggtg ttgcgctcga tgagccgcgt gaacacaccg cccaacgtct
     2701 cgatgccgag cgatagcggc gtcacgtcga gcagcagcac gtccttgacc tcgccctgga
     2761 ggacgccggc ctggatcgcc gcgccgacag caacaacctc gtccgggttg acgcccttgt
     2821 ggggctcctt gccgaacagc tccttcacga cctgctgcac gcgcggcatg cgggtcatac
     2881 cgccgacgag gacgacctcg tcgatctcag cggccttcaa cccggcatct ttcagcgcct
     2941 gctcacacgg gccgcgcgtc ttcgcgatca gatcctcgac caggctctcc agcttggcgc
     3001 gggtgagctt catggtcagg tgcttcggac ccgactggtc agcggtgatg aacggcaggt
     3061 tg
//
LOCUS       DASUMG010000095         2755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_93530, whole genome shotgun sequence.
ACCESSION   DASUMG010000095 DASUMG010000000
VERSION     DASUMG010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93530"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFX46_01555"
     CDS             <1..896
                     /locus_tag="VFX46_01555"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HEX5599302.1"
                     /translation="AREMEKAGVHVVYGFVELKTHAKLGLVVRREGNDIATYCHIGTG
                     NYHPQTARIYTDLSFFTADRAIGRDVARIFNFVTGYGKPQDLEVMAASPNGIRARILE
                     HIYEEIEHAKAGRPAAIWAKMNALVDPQIISALYEASQAGVEIDLVVRGICCLRPGIP
                     GLSENIRVKSLIGRFLEHSRIYCFGRGAGLPSPKAAVYISSADMMPRNLDRRVEAMVP
                     ITNPTVHEQILDQIMLANLKDNQQSWQILPDGSSVRIKPAPGEEPFNAHQYFMTNPSL
                     SGRGQALKENMPPRVRLTVRD"
     gene            913..2439
                     /locus_tag="VFX46_01560"
     CDS             913..2439
                     /locus_tag="VFX46_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX5599303.1"
                     /translation="MEETRGLLGRVRMAPLEPVGVIDIGSNSVRLVVYEGAVRAPTPL
                     FNEKVLCGLGRSIATTGKLESQAVERALGALRRFRAITELLAVKRLTAVATAAVRDAQ
                     NGAEFIARAEEACGFPIQLLPGEREAELAAQGILMGFGHVDGVAGDLGGGSLELIDIG
                     GFQLRRAATLPLGGLRLLDVSRGNLEDALKFIDAELDRHNWIGADRGRTFYAVGGTWR
                     ALARVHMAERDYPLRVMQGYAISPHGAIALCEDLRNGKKLKGLETVARARREVLPVGA
                     LVMERLLRRLEPDKVVFSVFGIREGLLLDLLPEDERKKDPLLSFCEDFARLRSRSAEH
                     GRELAAWTDALFTEPGPTETPEERRLRHAACLISDVGWRAHPDYRGEQSLNLIAHSAM
                     TGIDHPGRLFLAMAVYFRHVGGAEASPDELSSRIRRMMNKRDLRRARILGAALRTAHM
                     ISVGRPGIIPHTPLSYEDGRLVLTIPPAYAALDGERLRRRFASLAALLDMTGEVSFRT
                     "
     gene            2532..>2755
                     /locus_tag="VFX46_01565"
     CDS             2532..>2755
                     /locus_tag="VFX46_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5599304.1"
                     /translation="MADDPYALLGVPKNASDEEIRRAFRKLAKELHPDLNPGDKAAVE
                     RFKKVSAAYELLSDPEKRRRYDRGEIDASG"
BASE COUNT          483 a          860 c          898 g          514 t
ORIGIN      
        1 tggcgcgtga gatggagaag gccggcgtcc acgtggtcta cgggttcgtc gagctcaaga
       61 cgcacgccaa gctcggccta gtcgtccgcc gtgagggaaa cgacatcgcg acctactgcc
      121 atatcggcac ggggaactat cacccacaaa cggcgcgcat ttacactgac ttgtcgttct
      181 tcactgccga ccgcgccatc ggccgcgatg tggcgcgcat ctttaacttc gtcaccggtt
      241 acggcaagcc gcaggacctc gaggtgatgg cggccagccc gaacggcatc cgtgcgcgta
      301 tcctcgagca catttacgag gagatcgagc acgcgaaagc gggccggccg gcagcgattt
      361 gggcgaagat gaatgccctg gtcgatcctc agatcatctc cgcgctgtac gaagcaagcc
      421 aggccggcgt tgagatcgac ctcgtcgtgc gtggcatctg ctgcctgcgg cccggcattc
      481 ctggtctttc cgagaatatt cgagtgaaaa gcctgatcgg ccgcttccta gagcactcgc
      541 gcatctactg cttcggccgc ggcgcgggcc tgccgtcgcc gaaagcggcc gtctacatca
      601 gctccgcgga catgatgccg cgcaacctcg acaggcgggt tgaggctatg gtgccgatca
      661 ccaatccgac cgtccacgag cagatcctcg atcagatcat gctggccaac ttgaaggaca
      721 atcagcagag ctggcagatc ctgccggatg gctcgtcggt gcggatcaag ccggcgccgg
      781 gcgaggaacc gttcaacgcg caccagtatt tcatgacgaa tccaagtctt tctggccgcg
      841 gacaagcgct caaagagaat atgcctccgc gcgttcggct tactgtcagg gactaggtgc
      901 ggcaggtttg tgatggaaga aacgcggggc ctcttgggcc gggtgcggat ggctccgctg
      961 gagccagtgg gcgtaatcga catcggctcc aactctgtgc ggcttgtggt ctacgagggc
     1021 gccgtccgtg cgccgacgcc gctgttcaac gaaaaggttc tctgcggact agggcgttcg
     1081 atcgcgacta cgggaaagct cgaatcccaa gccgtcgagc gtgcccttgg agccttgcgg
     1141 cggtttcgag cgattaccga gctgcttgcc gtcaagcggc taaccgccgt ggcaaccgcc
     1201 gctgttcgcg atgcgcagaa cggtgctgag ttcattgcca gagccgagga ggcgtgtggc
     1261 ttcccgatcc agctcttgcc gggcgagcgc gaggcggagc tcgcggctca agggattctg
     1321 atgggcttcg ggcacgtcga cggcgtggca ggagaccttg gaggcggcag tctggagctc
     1381 atcgacatcg ggggatttca gctccgccgc gctgcgacgc tgcccctcgg cggcttgcgc
     1441 ttgctggacg tcagccgggg caacttggaa gacgctctga aattcatcga cgccgaactt
     1501 gatcgacata actggatcgg cgcggatcgc gggcgcacct tctacgccgt gggcggaact
     1561 tggcgggcac tcgcccgcgt gcacatggcc gagcgtgact atccgctccg tgtcatgcag
     1621 ggctacgcga tctccccgca cggggcgatc gccctctgcg aggaccttcg caacggcaag
     1681 aagctcaagg gactcgagac ggtcgccagg gcacggcggg aggtgctgcc tgtcggcgct
     1741 cttgtcatgg agcgcctgct gcggcgcctc gagcctgaca aggtggtgtt ctcggtcttc
     1801 ggcattcgcg agggcttgct actcgacttg cttccggaag acgagcggaa gaaggatcct
     1861 ctgctcagct tctgcgaaga cttcgcccgc cttcgctcgc gctcggccga gcacggccgc
     1921 gagcttgcgg cgtggacgga tgcgctgttc acagagccgg ggccgaccga aacacccgag
     1981 gaacgccgtt tgcgtcacgc cgcctgcctg atctcggacg tcggctggcg cgcccatcct
     2041 gactaccgcg gcgagcagag ccttaatctc atcgcccatt cggcgatgac gggcattgac
     2101 catcccgggc ggctgttcct ggccatggcc gtgtatttcc gccacgtcgg cggtgctgag
     2161 gcatcgcccg atgagctgtc ctcccggatt cggcgcatga tgaacaagcg tgatctgcgc
     2221 cgggcccgca tccttggtgc cgcgctgcgc acagcccata tgatttccgt ggggaggccc
     2281 ggcatcattc cgcatacgcc gctttcctac gaggacggtc ggctcgtcct cacgatcccg
     2341 ccggcctatg ctgcgcttga cggcgaacgt ctgcgccgcc gtttcgcgtc gcttgccgct
     2401 ctgctcgaca tgacggggga agtaagcttt agaacgtgat cggtggcgtg tagcttcgcg
     2461 cctgcggcgc ggaccgcgga atggtgctaa gctctccaag atgacatgag acagcgctag
     2521 tcttgtgagc catggccgac gatccttacg cgcttctggg agtccccaag aacgcctccg
     2581 acgaagaaat acgccgggcg tttcgcaagc tggcgaaaga gttgcacccg gacctcaatc
     2641 cgggggacaa ggcagctgtc gagcgcttca agaaggtgtc ggccgcctat gagctgctaa
     2701 gcgatcccga gaagcgcagg cgttatgatc gcggcgagat cgatgcctct ggcga
//
LOCUS       DASUMG010000096         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_655868, whole genome shotgun sequence.
ACCESSION   DASUMG010000096 DASUMG010000000
VERSION     DASUMG010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655868"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..2027)
                     /gene="hyfB"
                     /locus_tag="VFX46_01570"
     CDS             complement(15..2027)
                     /gene="hyfB"
                     /locus_tag="VFX46_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HEX5599305.1"
                     /translation="MAIGAVLTCVAALLALAAVGVVGHNRPIATRIVYSASLALSSVI
                     LIVAIVRLLTWPGPDPATTLPIGLPWIGARFRIDALSAFFLIVVNFGAAAASLYALGY
                     GEHEQQPGRVLPFYPAFLAGMNVVVLADDAFSFLVAWEFMSLASWALVVAHHQEAENT
                     RAGYIYLLMASFGTLTLLLTFGLLAGPAGGYGFDDIRAAQHASWLPGLVLALALIGAG
                     SKAGLVPLHVWLPLAHPAAPSHVSALMSGVMTKVAVYGFVRIVFDLLGGPSWWAGMIV
                     LALGGITAVMGVLYALMQHDLKRLLAYHTVENIGIIFIGLGLALAFGANGMQAAAALA
                     LTAALFHVLNHSLFKSLLFFGAGAVLTATGERDMEHLGGLIHRMPLTSFAFLAGCVAI
                     SALPPLNGFVSEWLTFQAILQSPDLPQWGLKILVPAVGGLLALSAALAATCFVKAFGV
                     TFLGRPRSGAAMRAEEVDEFSLSTMLGLAVLCLILGILPGLVIDALAPVTLSLTGGRM
                     PAQLGVAWLSIVPIAESRSSYNGLLVFLFIAISAWVAVLVIHRYASHALRRGPAWDCG
                     YPDFTPVTQYTASSFAQPIRRVFGTLVFRAREQVEMPPPGSPEPAHLTVKLRDLVWDG
                     AYVPIADAVSLAADRLNVLQFLTIRRYLSLVFGALVALLLALVLWP"
BASE COUNT          450 a          648 c          717 g          267 t
ORIGIN      
        1 gaggaggtcc tggatcacgg ccacagcacg agcgccagca gcagggcgac cagcgcgccg
       61 aacaccaggc tgaggtagcg ccggatggtc aggaactgca gaacgttgag gcgatccgca
      121 gccaagctga cggcgtcggc aatcggcacg taggcgccgt cccaaacgag atcccgcaat
      181 ttgacggtga gatgcgccgg ctcagggctg ccaggcggtg gcatctcgac ctgctcgcgg
      241 gcgcggaaga cgagcgtgcc gaagacgcga cggatgggtt gggcgaagct gctggccgta
      301 tactgtgtga ccggagtgaa atcggggtag ccgcagtccc aggcgggccc tcgccgcaac
      361 gcgtgggagg cgtagcggtg gatgaccagc accgctaccc aggccgagat ggcgatgaaa
      421 aggaacacca ggagcccatt ataggagctc cggctttcgg cgatcgggac gattgagagc
      481 catgccacgc ccaattgcgc gggcatccgc ccgccagtca gggacagcgt aaccggcgcc
      541 agcgcgtcga tgacaaggcc gggcagaatg ccgaggatga ggcagagcac ggcgagcccc
      601 agcatggtgc tgagcgagaa ctcgtcgacc tcctcggccc tcatcgccgc cccagagcgt
      661 ggccggccga ggaaggtcac gccaaacgcc ttgacgaagc aggtggcggc aagcgctgcc
      721 gaaagtgcca gcagcccgcc caccgcgggc accaggatct tcaggcccca ctgcggcagg
      781 tcagggcttt gcaagatcgc ctggaacgtc agccactccg agacgaagcc gttgagcggc
      841 ggcagcgccg agatcgcgac gcaaccggcg agaaacgcga agctcgtcag cggcatccga
      901 tgaataaggc cgccaaggtg ctccatgtca cgctcgcccg tggccgtcag cacggcgcct
      961 gcgccgaaga acagcaggct cttgaacagc gagtgattga gcacgtggaa gagcgccgcg
     1021 gtgagcgcca atgctgccgc tgcttgcatg ccattggcgc cgaaggcaag tgcgagcccc
     1081 agcccgatga agatgatccc gatgttctcg acggtgtggt aggcgagcag ccgcttcagg
     1141 tcgtgctgca tcaaggcgta aagcacgccc atgacggcgg tgatgcctcc gagagcaagc
     1201 acgatcatgc ccgcccacca gctcggccct ccgagcaggt cgaagacgat gcgcacgaag
     1261 ccatagacgg caactttcgt cataacgccg ctcatcaatg ccgagacgtg gctcggggcg
     1321 gcagggtgcg cgaggggcag ccagacgtgc agcggcacga gacccgcctt cgatccagcg
     1381 ccaatcagcg ccagcgcgag gacgagccca ggcaaccagg atgcgtgctg agccgcgcgg
     1441 atatcgtcga atccgtagcc gcccgcagga ccggcgagca ggccgaaggt gaggagcagc
     1501 gtcagcgtgc cgaagctcgc catcagcagg tagatgtagc ccgcgcgcgt gttctccgcc
     1561 tcctgatggt gcgccacgac gagcgcccag gaggcgagcg acatgaactc ccaggcgacg
     1621 aggaagctga aggcatcatc ggcgagtacg acgacattca ttccggcaag aaacgccgga
     1681 tagaacggca gcactcgtcc gggttgctgc tcgtgctcac cgtagccgag tgcataaagg
     1741 ctcgcggcag cggcgccgaa attgacgacg atgagaaaga aagccgaaag agcatcgatt
     1801 cgaaatcgcg caccgatcca gggcagcccg atgggcagag tggtcgcggg atccgggccg
     1861 ggccacgtca gcagccggac gatcgcaacg atcaggatga ccgaggagag agcgagagaa
     1921 gcggagtaaa cgatgcgagt ggcgatgggt ctgttgtgcc cgaccacacc gaccgccgcg
     1981 agcgcaagca gtgccgccac gcacgtgagc accgcaccga tcgccatttg cacttatctc
     2041 cgcatcttta ggctgtggcc gggcaggagg gcgttaggcc cg
//
LOCUS       DASUMG010000097         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1057172, whole genome shotgun sequence.
ACCESSION   DASUMG010000097 DASUMG010000000
VERSION     DASUMG010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057172"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1683
                     /gene="istA"
                     /locus_tag="VFX46_01575"
     CDS             184..1683
                     /gene="istA"
                     /locus_tag="VFX46_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX5599306.1"
                     /translation="MFAVEVYAAVRHFVFVEGNSRREAARVFGLSRETVLKMCRFSLP
                     PGYTRTKPVMKPKLGALLPVIDAILEADRTGPVKQRHTAKRIFERLRDEHGFGGGYTV
                     VKDYVRIARARGRETFVPLSHPAGHAQVDFGEAVGVIGGVRCKLHFFCMDLPQSDACF
                     VKAYPAETTEAFLDGHVSAFAFFGGVPLSILYDNLKIAVAKICGDGKRERTRAFTELV
                     SHYLFQDRFGRPGKGNDKGKVEGLVKYARSNFLTPIPVAASFDDLNAMLAERCRARQN
                     ERAGRHAANIGERLAADLAAFRDLPAVPLEPCEKRAARVSSTALVRYRGNDYSVPTAF
                     GFQDVMVKGFVDQTVILCGGEEIARHQRCYGSGTFVFNPLHYLALIEMKPNALDQAAP
                     LQDWSLPEAFQNLRHLMEARMGNRGKREFIQVLRLMEAIPKDVVVYAVTEAIRIGAIG
                     FDAVKQIALARIERRPARLDLSAYPHLPKTSVKTTSAADYAVLVPGRAA"
     gene            1695..2522
                     /gene="istB"
                     /locus_tag="VFX46_01580"
     CDS             1695..2522
                     /gene="istB"
                     /locus_tag="VFX46_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX5599307.1"
                     /translation="MDMMPTIGTPQVLLSHHLKQLKLPTVLREYDKVARECARDGVDH
                     PRYLLRLVELELIDRERRVVERRIKQARFPAVKSLDTFDFTAIPSLNKMLVLELARCE
                     FILRRENIVALGNSGTGKTHVALALGLAACQKGFPVAFTTAAALVHQLMEARDEKRIL
                     RLQRELQTVKLLIVDELGYVPLSPTGAELLFEVLSQRYERGSTIVTSNLPFEDWTSVL
                     GSERLTGALLDRLTHHVHILTMNGDSYRLKQSTGRRPAPAAEQNQTTPETADQPKSA"
BASE COUNT          484 a          853 c          885 g          504 t
ORIGIN      
        1 agagatagac cgcggctggc ggatccggtc cgttccaagg ccggtcatcg cgggcgtaca
       61 cccacaaccg gccagtgtgc gtggcggtga caaagtaggc cacggagcgc cggcgtgatg
      121 tcggtgcggg cggagtaaaa accgtccagt ggctaggttg atccctttgg gggtcggccg
      181 ggaatgtttg ccgtggaagt ctacgccgcc gtccggcatt tcgtctttgt cgagggcaac
      241 agccgtcgtg aggcggcccg ggtattcggg ctgagccgcg agacggtctt gaagatgtgc
      301 cgcttctcgc tgccgcctgg ctacacgcgc acgaagcctg tgatgaagcc gaagctgggc
      361 gcgctgctgc cggtgatcga tgcgatcctg gaagcggacc ggaccggccc ggtgaagcag
      421 cgacacacgg cgaagcggat tttcgagcgg ctgcgcgacg agcatgggtt cggcggtggc
      481 tacacggtgg tgaaggacta tgtccggatc gcgcgggcac ggggccgcga gacgttcgtg
      541 ccgctgtcgc atcctgcggg ccatgcgcag gtggactttg gcgaggcggt cggtgtgatc
      601 ggcggggttc gctgcaagct tcacttcttc tgcatggacc tgccgcaatc ggacgcctgt
      661 tttgtcaagg cctatccggc cgagaccacg gaagcgttcc tggacggtca cgtctctgcc
      721 ttcgccttct tcggcggcgt gccgctatcg atcctctacg acaatctcaa gattgcggtg
      781 gccaagatct gcggcgacgg caagcgcgag cggacgcggg cattcaccga gttggtgagc
      841 cactatctgt tccaggatcg ctttggccgt cccggcaagg ggaacgataa gggcaaggtc
      901 gagggattgg tgaagtatgc ccgctcgaac ttcctgacgc cgatcccggt ggcggcgagc
      961 tttgatgatc tcaacgccat gctggccgaa cgctgccggg cgcggcagaa cgaacgtgcc
     1021 ggacgccatg ccgcgaacat tggtgaacgc cttgccgccg atctcgccgc gttccgggat
     1081 ttgccagccg tgccgctgga gccctgcgag aagcgggcgg cccgggtctc gtcgacggcg
     1141 ctagtgcgct atcgcggcaa tgactactcg gtgccgacgg cctttggctt ccaggacgtc
     1201 atggtgaagg gcttcgtcga tcagaccgtc atcctgtgcg gcggcgagga gatcgcgcgg
     1261 catcagcgct gttatggttc gggcaccttc gtcttcaacc cgctgcacta tctggcgctg
     1321 atcgagatga agccgaacgc gctcgatcag gccgcaccgt tgcaggactg gagcctgccc
     1381 gaggcgttcc agaacctgcg tcatctgatg gaggcgagga tgggcaatcg cggcaaacgc
     1441 gagttcatcc aggtgctgcg gctgatggag gcaatcccga aggatgtcgt ggtctacgcc
     1501 gtcaccgagg cgatccggat cggcgcgatc ggattcgatg cggtcaagca gatcgctctg
     1561 gcccgcattg agcgccgtcc tgcgcgactc gatctcagcg cctatcccca tctgccgaag
     1621 accagcgtga agacgacatc ggcggccgat tacgccgtcc tcgttcccgg gagggcggca
     1681 tgagtgggaa ggccatggat atgatgccga cgatcggcac gccgcaggtg ctgctcagcc
     1741 atcatctcaa gcaattgaag ctgccgacgg tgctgcgcga atacgacaag gtcgcccgtg
     1801 aatgcgcgcg cgacggcgtc gatcatcccc gctatctgct gcgcctggtc gaactggaac
     1861 tgatcgaccg ggaacggcgt gtcgtcgagc ggcgcatcaa gcaggccagg ttcccggcgg
     1921 tgaagagcct ggacaccttc gacttcaccg ccattccttc cctcaacaag atgctggtgc
     1981 tggagctggc acgctgcgag ttcatcctgc gtcgcgagaa catcgtcgcg cttggcaatt
     2041 ccggcaccgg caagacccat gtcgcgctcg cccttggcct tgccgcctgc cagaagggct
     2101 tccccgtcgc cttcaccacc gcggctgccc tcgtgcatca gctcatggag gcccgcgacg
     2161 agaaacggat tttgaggctg cagcgtgaac tgcagacggt gaagctgctc attgtcgatg
     2221 aactgggcta cgtgccgctg tcaccgaccg gcgcggaatt attgttcgag gtgctctccc
     2281 agcgctacga gcgcggatcg acaatcgtca cctcaaactt gccttttgaa gactggacct
     2341 cggtcctggg gtccgagcgg ctcactggtg cgctgctcga tcggctgacc caccacgttc
     2401 acatcctcac catgaatggc gacagctacc ggctcaagca atccaccgga cggcgtcccg
     2461 cccctgcggc ggagcaaaac cagaccaccc ccgaaacagc cgatcagcca aaaagcgcct
     2521 gatccacaac agccacgcca aatcctgacg accgctcgct acagcgctag ggaaaagcgc
     2581 gcttcgcgag cggcctctgc cgcgcccaga ctggcctgct tttactccgc ctccgtggct
     2641 tggttttgct ccgccgttga cactcgaagc aaagctctca gggttcactt ggcgcgacac
     2701 catagagtat ggctgggcag ctcatg
//
LOCUS       DASUMG010000098         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_414632, whole genome shotgun sequence.
ACCESSION   DASUMG010000098 DASUMG010000000
VERSION     DASUMG010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414632"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFX46_01585"
     CDS             complement(<1..758)
                     /locus_tag="VFX46_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HEX5599308.1"
                     /translation="MTSADEIFSIYAQSYDRQKQHEMPLRQYLEACRDDPMLYASAAE
                     RMMVAIGEPTLVDTSKDPRLGRIFQNRTIKIYPTFKDFYGLEETIERIVGFFRHAAQG
                     LEERKQILYLLGPVGGGKSSLAERLKELMEQCPVYVLKAGDEISPIFESPLGLFDPVT
                     MGPVLEEKYGIPQRKLTGRMSPWAVKRLDEFGGDISRFNVVKLYPSRLRQICVAKTEP
                     GDENNQDVASLVGKVDIRKLEMYSQNDPDAYSFSG"
     gene            complement(1421..>3227)
                     /locus_tag="VFX46_01590"
     CDS             complement(1421..>3227)
                     /locus_tag="VFX46_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599309.1"
                     /translation="LKTEVEHSRLNLSRLAPEEIRQLLACNEAGSALSSTMRRRIAIR
                     SEGIPLFAVELARLCASASNNDRLDLLLEPGPLNTILSARLDSLGTRKSLIQAAAIVG
                     REFGVEVLGLVLQMNSSDLIDDLHRLERSGILESLPRRKGSARFRFSHVLLREAAYAS
                     VIEARRAELHFRTAEVLAKDERASDEAPEIIARHYAAAGDFKKAFTWWFRAGARASEM
                     AATRAAVHHLSQALAALRQDPGAGSPQEEVAVLRLLGAQLVSLKGNAAPEAVDAFRRC
                     LDLSREAPGADGEFDALWALHTCHLVRGDIDEALKVGERLTASADRGGNEERRLRAHR
                     VQGLAKLLNGQLDAAFAHLRLALDLYDEARHANLRFQYASDQGALARAHLAWGEAIAG
                     LLDSSIQNAEAALALSSRLRHPHTSAHVLCVLAARAQTLGDQATASALAYAGRTIAER
                     HEFPYWVAWADIILGWSQRQAGDLGYEEAIQQIENAIRAYRRTGATQALPYALLLLAD
                     VALTYDRPHRALSAVAEGWQLAERYGLMLYASELLRIRAVAELRSGSDLSDVSKTLEQ
                     AEQLATRQGAKTFFCRIAEFRIHSEPRQMVSRRET"
BASE COUNT          613 a         1009 c          962 g          643 t
ORIGIN      
        1 ccggagaatg aataggcgtc gggatcgttc tggctgtaca tttcgagctt gcggatatcg
       61 accttgccga ccaggctggc gacatcctgg ttgttctcgt cgccgggctc ggtcttggcg
      121 acgcagatct ggcggagccg ggacgggtag agcttgacga cgttgaacct ggagatgtcg
      181 cccccgaact cgtccaaccg cttgaccgcc cacggcgaca tgcgccccgt cagcttgcgc
      241 tgggggatgc catacttctc ctcgagcacc ggtcccatgg tcacggggtc gaagaggccg
      301 agcgggctct cgaaaatcgg gctgatctcg tcacccgcct tgagcacgta gaccgggcat
      361 tgctccatca gctccttgag ccgctccgcc agggacgact taccaccccc gaccggcccc
      421 aggaggtaga ggatctgctt acgttcctcc aagccctgcg cggcatggcg gaagaagccg
      481 acgatcctct cgatcgtttc ctcgagcccg tagaagtcct tgaaagtcgg ataaattttt
      541 atcgtccgat tttggaagat gcggccgagg cgcggatcct tcgacgtgtc gacgagggtc
      601 ggctcgccga tcgccaccat catccgttcg gccgcactgg cgtacagcat cggatcgtcg
      661 cggcaggctt cgagatactg ccgcagcggc atctcatgct gcttctggcg atcgtagctc
      721 tgcgcgtaga tgctgaaaat ctcatctgcg gaggtcatgc ttgctctcgc tctcgcgatc
      781 tcaccactct cgtgatgacg tggcctcccg ccatcaccca ttaaacttga agtgggctct
      841 ctcaagccac ttggcaatgt gcttgcccgc ttcaaccgcc actacttctt catcaccgcc
      901 ttgtgtcatc ctaacacagt cgagctgcat ctcgattgcg gccaacgtga attcgttttc
      961 ttttccgatc cagcacgcaa tgggctggcc actatggcgc ccacttctgg cagaagtggg
     1021 tcgctgcaac gatcgtgccg cactgcgacg gctgatccac cgatgttaat gaacggcttt
     1081 ccggtctgct ccggacactc gaaacggacg cagctagtca aacgcacgct cgcacttttt
     1141 ggttcggcca aagcgtcaat tggcgcggat cggtatcgtc gctgcacccc gtccggcgtc
     1201 aagacccgat accctgctgt ctcgaccgca cttgcgatcg tttcgtccat ctcgaccata
     1261 aactgattcc gggtatcaga atgctgcgca atcacgtcta aagtggagga cgcggcgagg
     1321 catctgaagt tcccggcggc acgtgcccac gcccgatttc aatgcaaaaa tgccgaattt
     1381 ttgcacaatt tccggaactt gatatccggt gtcagacgtt tcaggtttcc cgtctagaaa
     1441 ccatttgccg tggttcggag tgtatccgaa attccgcaat ccggcagaaa aatgtttttg
     1501 ctccctgccg agttgccagt tgttctgctt gttcgagggt ttttgagacg tcgctcaaat
     1561 ccgatccgga gcgaagctcg gccacggcgc ggatccgaag cagttcggac gcatagagca
     1621 taaggccata ccgctcggcg agctgccatc cttcggcaac agccgacaag gcacggtgcg
     1681 gacgatcata ggtgagcgcc acatcggcca aaagtaggag ggcatagggt aaggcctgag
     1741 tagcgcctgt gcggcgataa gccctgatcg cattctcaat ctgttgaatt gcttcctcgt
     1801 acccgaggtc gccggcctgc ctttggctcc agccgaggat gatgtccgcc caggcgaccc
     1861 agtagggaaa ctcatgccgc tcggcgatgg tcctgccggc ataggcgaga gccgatgcgg
     1921 ttgcctggtc gccgagcgtc tgcgcccgcg ccgcaagcac acacagcaca tgcgcgctgg
     1981 tgtgaggatg acgcagccgg gaagacagcg caagggccgc ttcggcattc tggatcgagc
     2041 tgtccaggag ccccgcaatg gcttcacccc aggcaagatg agcccgggcc agcgcaccct
     2101 gatctgatgc gtattggaac cgcaggttcg cgtggcgggc ctcatcgtag aggtccaggg
     2161 caagcctgag gtgcgcgaag gcggcatcga gttgcccgtt tagaagtttg gcgaggccct
     2221 gaacccggtg tgcacgcaaa cggcgctctt cgttaccgcc cctgtcggcg ctggccgtca
     2281 ggcgctcccc aaccttgagc gcctcgtcaa tgtctcctcg aacgaggtga caggtgtgta
     2341 gagcccaaag tgcatcgaac tcaccatccg cacccggggc ttcacgagac aggtcgagac
     2401 agcgccggaa ggcgtcgacg gcctccggcg ctgcgttccc cttgagcgat acgagctggg
     2461 caccgagcag gcggagcact gcgacttcct cctgcgggct gccagcacct gggtcctgcc
     2521 gcaaggcagc gagcgcctga ctgagatggt ggacagctgc ccgcgtcgcg gccatctcgg
     2581 aagccctcgc gcccgccctg aaccaccatg tgaatgcctt cttgaagtct cccgcagcag
     2641 cgtagtgccg ggcgataatc tctggggcct cgtccgatgc acgttcgtcc ttcgccagta
     2701 cctcggcagt gcgaaaatgc agctcggcgc gtcgagcctc gatcaccgat gcataggccg
     2761 cctcccggag gagcacatgg ctgaagcgga accgggctga ccctttccgc cgaggcagag
     2821 actcgaggat gcccgatctc tccaggcgat gcagatcatc gatgagatcg gaggagttca
     2881 tctgcaacac gaggcccagc acctcgacgc caaactcgcg cccgacaata gcggccgctt
     2941 ggatcaacga cttccgggtg ccgagcgaat caagccgtgc cgacaggatc gtgttcaggg
     3001 gtccgggttc caggagcagg tccaaacgat cgttgttgga agcgctggcg cacaaccgcg
     3061 cgagctccac tgcgaacagt ggaatgccct cggaacggat ggcaattcgt cggcgcatgg
     3121 tggatgacag cgccgatccc gcctcgttac aggcgaggag ctgcctgatt tcttcgggtg
     3181 ccagcctcga gaggtttagc cgagagtgtt ccacttcggt cttgaga
//
LOCUS       DASUMG010000099         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_575273, whole genome shotgun sequence.
ACCESSION   DASUMG010000099 DASUMG010000000
VERSION     DASUMG010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575273"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VFX46_01595"
     CDS             complement(<1..557)
                     /locus_tag="VFX46_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX5599310.1"
                     /translation="MPRAAVLVLDSVGVGGAPDAALYDDAGADTVGHIAEWCARGEGD
                     REGLRAGPLKLPNLVSLGLGEACRLATGRIPPGLEGEIRDACYGSAIETSRGKDTPSG
                     HWEIAGSPVTFEWGYFPKDSPCFPPEIVDALCEEAHLPGILGNCHAAGTAIINVLGEE
                     HLRTGKPICYTSVDSVFQIAAHEEV"
     gene            complement(620..1540)
                     /locus_tag="VFX46_01600"
     CDS             complement(620..1540)
                     /locus_tag="VFX46_01600"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5599311.1"
                     /translation="MAEIKSPLPKSEVSTRLLDPLEACASGDLAPNVALMRLMIDAQS
                     PSEIDELLKAIDGAGSLGSLKALWSQTANAWETVKFVLDNVNHGSSRSDSRDPEYWAD
                     RFDQLAENAPEAGIALYSLGSPILLELATVSLVDRLQSWGLLHPSSVVLDLGCGMGRV
                     CEALVPRVRAVVGIDVSRRMLALARRRCINCRNVFFVQTSGRDLAAFADASFDLVLAV
                     DTFPYLVLSSSASAATHLSEARRVLKPGGSLVAFNYAYGVDDRTAAEMFCQHAKAAAL
                     EPVRLGTRDLAWWDGVTYHVRRASRELTPL"
     gene            complement(1533..>2168)
                     /locus_tag="VFX46_01605"
     CDS             complement(1533..>2168)
                     /locus_tag="VFX46_01605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5599312.1"
                     /translation="PNAAALALPCPANCSDSTEDDEICPVLDLFTGPKDAGTHPAIPA
                     LQRRKLRMARHRLERGPSSTVLSTQDLSADEWLDVLITLHGARWRERAMPGVLADPQV
                     QAFHRQVLPQFISIGIARLFALKIGDDVAGVYYGFSHRNRAYAYLGGFDPRFSYYSPG
                     TVLLGHAIEDALRNGAREFHFLRGREPYKRAWGAHDQKTFRRKFVKQSPHG"
BASE COUNT          476 a          638 c          672 g          382 t
ORIGIN      
        1 aacacttcct catgggcagc tatctgaaaa acgctgtcca ccgacgtgta gcagatgggc
       61 tttcccgttc ttaaatgctc ctcgccgaga acattgatga tggcagttcc cgcggcgtgg
      121 cagttgccga gaatccccgg cagatgcgcc tcttcgcaaa gggcatctac gatctcgggc
      181 ggaaagcagg gactatcctt cggaaaatag ccccactcga acgtcacggg cgagccggcg
      241 atctcccagt ggccggatgg cgtgtctttg ccgcgggatg tctcgatggc ggagccgtaa
      301 catgcgtccc gaatttcgcc ttcgaggccg ggaggaatgc gacctgtggc aaggcggcag
      361 gcttcgccaa gcccaagact gacgagattg ggcagcttta gcggtccagc acgcaacccc
      421 tcgcgatcac cttcgccgcg cgcgcaccac tcggcgatat ggccgacggt gtcggcgcct
      481 gcgtcgtcgt agagggccgc atccggtgca ccgccaacgc cgaccgaatc caggacgagg
      541 accgctgcac gaggcatagg gttcgaacct tctgttcaga tccaagggga ttcaccaaca
      601 ccatggacac ccggtttgtt cagagtgggg taagctctcg gctggcacgt cggacatgat
      661 aggtgacccc atcccaccaa gcgagatcgc gcgtgccgag tcgcacgggc tcgagagcgg
      721 cagccttagc atgctggcaa aacatctctg cggcggtccg gtcatcaacg ccataagcgt
      781 agttgaaggc gaccagcgag ccacccggct tgagcacgcg gcgggcctcg ctgagatgcg
      841 ttgcggccga ggcagagctg gacaggacga gataggggaa tgtgtcaacc gccagcacca
      901 ggtcgaatga cgcgtcggcg aaagcagcga ggtctcgccc ggaagtctgc acgaagaaaa
      961 cattgcggca gttgatgcag cgccggcggg caagcgccag catacggcgc gatacatcga
     1021 tgccgaccac agcgcgaacg cgcggcacaa gtgcctcgca aacgcggccc attccgcagc
     1081 ccagatccag cacgacagaa ctcgggtgaa gaagacccca gctttgcagg cgatcgacga
     1141 ggctgactgt tgcgagctcg agcaagatag gcgagcccag cgaatagagc gcgatcccgg
     1201 cctcgggtgc attttcggcg agctgatcaa atcgatcagc ccaatattcc ggatcgcgtg
     1261 agtccgagcg cgaggatcca tgattgacat tatcgagcac gaatttgaca gtctcccacg
     1321 cgtttgctgt ctggctccaa agggccttta ggctgccgag acttccagcg ccatcgatgg
     1381 ccttcagcaa ctcgtcgatt tcgcttgggc tctgtgcgtc gatcatcagc cgcatcaagg
     1441 cgacattcgg ggccagatca ccgctggcac acgcctcaag cggatccagc agacgagtgg
     1501 aaacttcaga tttaggaaga gggcttttta tctcagccat gaggggattg cttcacaaac
     1561 tttcgccgga aggttttctg atcgtgcgcg ccccaggctc tcttgtacgg ctcacgtcca
     1621 cgaaggaaat gaaattcgcg cgcgccattc cgaagcgcat cttcgatagc gtggccgagg
     1681 agcactgtac cgggactgta gtaggaaaag cggggatcga agccgccgag ataggcgtag
     1741 gcgcggttac gatgcgaaaa gccgtaataa acaccggcaa cgtcgtcgcc gattttcaag
     1801 gcaaaaagcc gggcaatgcc gatcgaaata aactgtggaa gcacttggcg atgaaacgcc
     1861 tgcacctgcg gatcagccag gactccgggc attgcgcgct ctcgccaacg agccccatga
     1921 agggtgatca gaacatccag ccactcatcg gcagacaggt cttgcgtgct caagacagtg
     1981 gagcttggcc cgcgttccag tctgtggcgg gccatgcgca gcttacgccg ttgcagagcc
     2041 ggaatggcgg gatgcgtgcc agcgtccttg ggccctgtga agagatcgag aacggggcaa
     2101 atttcgtcgt cctcagtgct atcactgcaa ttggcagggc aggggagcgc aagcgcagct
     2161 gcgttcgg
//
LOCUS       DASUMG010000100         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1057206, whole genome shotgun sequence.
ACCESSION   DASUMG010000100 DASUMG010000000
VERSION     DASUMG010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1057206"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VFX46_01610"
     CDS             <1..643
                     /locus_tag="VFX46_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX5599313.1"
                     /translation="VRVLLGLITPDRGEVRRRPGLSIGYVPQRFDIDRAIPMTVARFL
                     RLGRPGEPRQIQAVLEEVGAPHVLEQQLSNLSGGELQRVLLARALLRNPQLLVLDEPV
                     RGVDYAGEAELYTLIGRLRTNRGLGVLLVSHDLHVVMAQSDRVICVNRHICCSGVPET
                     VARDPSYVRLFGAHAAQAFAIYSHDHDHHHDLAGETHPAASGAEVGAKQQNGA"
     gene            649..1446
                     /locus_tag="VFX46_01615"
     CDS             649..1446
                     /locus_tag="VFX46_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX5599314.1"
                     /translation="MDPFLIRAFAAGIGVAILAAPLGCFVVWQRMAYFGEAVAQASLI
                     GIALGLAFHLDLTASALALSAAMALVLIVLARHALVPLDSLLGLLAHAALALGVIAAS
                     LVQGPTVDLLGYLFGDIFAVSTSDLWWVFGGGAIILLALAYLWQPLLSLAVHEELAAA
                     EGVRRNLVKGVFVVLLALAIAIIIKVVGILLAIAFLIMPAAAARPLSATPERMAVIAA
                     AIAAFGALAGIQLSVSYDIPGGPAIVLVLAMIAGTSLVGMAVRSGAR"
     gene            1531..2784
                     /gene="ispG"
                     /locus_tag="VFX46_01620"
                     /gene_synonym="gcpE"
     CDS             1531..2784
                     /gene="ispG"
                     /locus_tag="VFX46_01620"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX5599315.1"
                     /translation="MADLMSPRRKSVPVTVGGVFVGGHHPIVVQSMTNTDTADVEATT
                     QQVAALARAGSELVRITVDRDESAKAVPRIRERLDRMGIRVPLVGDFHYIGHKLLAEN
                     PACAEALDKYRINPGNVGFKNKRDLQFAAIIEQALKWGKPVRIGVNWGSLDQELLTHL
                     MDENAKSPEPKSARAVLHEAIVQSALRSAARAEELGLGRDRIIISAKVSAVQDLVTVY
                     TMLAERCSYALHLGLTEAGMGSKGIVASSAAMGILLQQGIGDTIRYSLTPEPGGDRTL
                     EVRAAQELLQTMGLRTFMPTVAACPGCGRTTSTVFQELARDVQEMIRERMPEWREKYP
                     GVETLNLAVMGCIVNGPGESKHADIGISLPGTGEQPAAPVFIDGKKAVTLRGPRIAEE
                     FKAIVEDYIEKRFGAAAREGRREAV"
     gene            complement(2791..>3151)
                     /locus_tag="VFX46_01625"
     CDS             complement(2791..>3151)
                     /locus_tag="VFX46_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5599316.1"
                     /translation="LKPRRPQLRMVAVEPALSPVLSGGTHSPHPLQGIGAGFIPEIMD
                     TSLIDEIITVENQPAIDMARKVARLDGIAVGISSGAAIWAAVQIGKRPEMAGKRIVTI
                     VPSFAERYLSTVLFEGL"
BASE COUNT          491 a         1032 c         1027 g          601 t
ORIGIN      
        1 cgttcgcgtt ctgctcggcc tcatcacacc cgaccgaggt gaggttcgcc gccgccctgg
       61 gctttcgatc ggctatgttc cccagcgttt cgatatcgac cgcgctattc cgatgacggt
      121 cgcccgcttt ctccggcttg gccgacccgg cgagccccgg cagatccaag ctgtgctgga
      181 agaagtcggc gctcctcatg tgttggagca gcagctctcg aacctctccg gtggcgagtt
      241 gcagcgcgtg ctgcttgcac gcgcgctgct ccgcaacccg cagctcctcg tcctcgatga
      301 gcctgtgcgc ggggtggact acgccgggga agcggaactg tacactttga ttggacgcct
      361 ccgcaccaac cgcgggttgg gcgtgctcct cgtgtcgcac gacttgcacg tggtcatggc
      421 gcaaagcgat cgcgtgatct gcgtcaaccg gcacatttgc tgctctggcg tgccggagac
      481 ggtcgcgcga gatccatcct atgtccggct gttcggcgct catgccgcac aagccttcgc
      541 catttacagc cacgatcacg accatcacca cgatctcgcg ggcgagaccc acccagccgc
      601 gtctggggcc gaagtgggcg cgaagcagca gaacggagcg tgattggcat ggacccattt
      661 ctgatccggg ccttcgccgc aggcattggc gttgccatcc tcgccgctcc gctcggctgc
      721 ttcgtggtct ggcagcgcat ggcctacttc ggcgaggccg tcgcccaggc gagcttgatc
      781 gggatcgccc ttgggctcgc gttccacctc gacctcacgg cttcggcgct cgcgctatcg
      841 gcagcgatgg cgctcgtgct catcgttctg gcgcggcacg ctctggtgcc gcttgattca
      901 ttgctcggcc tgctggccca cgcagcgctg gccttgggcg tgattgcggc ttctttggtg
      961 cagggtccaa cggtcgacct tctcggctat ttgttcggag acatttttgc cgtctcgacg
     1021 agcgatctct ggtgggtttt cggaggcgga gccatcattt tgctcgcact cgcttatctt
     1081 tggcagccgc tgctgtctct cgccgttcac gaggagctcg ctgcggcaga gggcgtccga
     1141 cgcaatctcg tgaagggggt tttcgtcgtg ctgctggcgc tcgcgatcgc catcatcatc
     1201 aaggtcgtgg gtatcctgct tgcgatcgct tttctcatca tgccggcggc tgccgcgcgg
     1261 ccgctcagcg cgaccccgga gcgcatggcc gtgattgcag ccgcgatcgc ggcttttggt
     1321 gcgttggcgg gcattcagct ttcggtgagc tacgacatcc cgggcggacc ggcgattgtg
     1381 ctggtgctgg cgatgatcgc cgggacatca ttggtcggta tggctgtgcg cagcggcgcc
     1441 cgttagaggg cctgtggtga cccgcccctg ttcaccgcgg cttacctgga ttaggtaaga
     1501 cttgcgagag accttggacc aggaacagtc atggctgatc tgatgagccc gcggcgcaag
     1561 tccgtgccgg tcactgtcgg cggcgtattc gtcggcgggc accatccgat cgtcgttcag
     1621 tcgatgacga acaccgacac cgctgacgtc gaggcaacga cgcagcaggt ggcggcgctc
     1681 gcgcgtgccg gctccgagct ggtgcgcatc acggtcgacc gcgacgagtc ggcgaaggcg
     1741 gtcccgcgta tccgcgagcg gctggaccgc atgggcatcc gggtgcccct cgtcggcgac
     1801 ttccattaca tcggccacaa gctgctcgcc gaaaatccgg cctgcgcgga ggcgctcgac
     1861 aagtaccgca tcaaccccgg caacgtcggt ttcaagaaca agcgcgacct gcagttcgcg
     1921 gcgatcatcg agcaggcgct gaaatgggga aagccggttc gcatcggcgt gaactggggt
     1981 agtctcgatc aggagctgct cacgcacctg atggacgaga acgccaagtc gcctgagccc
     2041 aagagcgcgc gcgccgtgct gcatgaggcg atcgtccagt ccgcgctgcg ctccgccgcc
     2101 cgcgccgagg agctggggct tgggcgcgac cgcatcatca tctcggccaa ggtttcagcc
     2161 gttcaggacc tcgtcaccgt ttacacgatg ctggccgagc ggtgctccta tgcgctgcac
     2221 ctgggtctca ccgaagcggg catgggctcc aagggcatcg tcgcctcgtc ggcagcgatg
     2281 ggcatcctgc tgcagcaagg catcggcgac acgatccgct actcgctcac gcctgagccc
     2341 ggcggcgatc gtacgctgga ggtccgcgct gcgcaggagc tgctgcagac aatgggcctg
     2401 cgcacgttca tgccgacggt tgccgcttgc cccggttgcg gccggactac ctcgacagtg
     2461 ttccaggagc tggcgcggga cgttcaggaa atgatccgcg agcggatgcc agaatggcgc
     2521 gagaagtacc ccggcgtcga aaccctgaac cttgcagtca tgggttgcat cgtgaacggc
     2581 cccggcgagt ccaagcacgc tgacattggc atctcgcttc ccggcacggg cgagcagccc
     2641 gcggccccgg tgttcatcga cggcaagaag gccgtgacgc tccgtggtcc acggatcgcg
     2701 gaggaattca aggcgatcgt cgaggactac atcgagaagc gtttcggggc agccgcgcgc
     2761 gagggacggc gcgaggcggt ctgatccgcc tcacagcccc tcgaacaaca ccgtcgacag
     2821 ataccgctcc gcgaaggacg gcacgatcgt cacgatgcgc ttgccggcca tctcaggccg
     2881 tttgccgatc tgaactgctg cccagatcgc ggcgccggac gagatgccaa cagcaatgcc
     2941 gtcgagccgg gcgactttgc gcgccatgtc gatggctggc tggttctcga ccgtgatgat
     3001 ctcgtcgatc agcgacgtgt ccatgatctc cggaatgaac ccggccccaa tgccctgcag
     3061 cgggtgagga ctgtgcgttc cgcccgacag caccgggctc aacgccggtt cgaccgcgac
     3121 catccgcaac tggggtcgcc tcggcttcag g
//
LOCUS       DASUMG010000101         2924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_736212, whole genome shotgun sequence.
ACCESSION   DASUMG010000101 DASUMG010000000
VERSION     DASUMG010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736212"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VFX46_01630"
     CDS             complement(<1..787)
                     /locus_tag="VFX46_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5599317.1"
                     /translation="MTHPLPLGLLAELTHRCPLACPYCSNPVELDRRSDELDSATWLR
                     VFDEAAALGVLQLHLSGGEPTARADIVALTRGASRAGLYTNLITSGVGLSEARLEELA
                     AAGLDHVQLSWQDTDPANADEIAGLAGAHAWKQRTVRAVIALGLPLTINVVVHRRNIA
                     RVGDMIVAAHRAGARRIEVAHAQYYGWALTHRAALLPSIEQVETAHTTVARLRQELDP
                     GILIDYVAPDHFSRWPKPCMNGWSRQSLNVTPRGKVLPCHAAES"
     gene            complement(784..1131)
                     /gene="pqqD"
                     /locus_tag="VFX46_01635"
     CDS             complement(784..1131)
                     /gene="pqqD"
                     /locus_tag="VFX46_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HEX5599318.1"
                     /translation="MARPSAAAWLARQARVSIGWTDMRARIAPDARPCLARGVRLHED
                     RARGRVVLLAPERVVAPNPSAIEILRRCSGTLTFAAIVEDLARSHQADPGRIAADTEK
                     LLLDLAYRRLVDL"
     gene            complement(1286..2539)
                     /locus_tag="VFX46_01640"
     CDS             complement(1286..2539)
                     /locus_tag="VFX46_01640"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX5599319.1"
                     /translation="MRRWIGSAHAVSFSLVLVGQTDTVLAQDHQIVDTDHVRVRVETI
                     ATGLHHPWGIALLPDGRYLVTERNSGDLRIGNLKGELSAPVEGVPDTFRYIGDTSSSQ
                     GGLFHVALHPQFAENRLVYLSYSQPSSEGAGTAISRGRLDESGDTPRLEDVELIFSMN
                     KHDSGGLHFGGRFVFDPRDNSIYLSIGDRRNMSRAQDPKDHAGSIVRITDEGQTPPDN
                     PFVNDPDKDEMIYSWGHRNIQGMAIHPQTGELWANEHGPLGGDEINLIKPGRNYGWPF
                     QTGGVDYSGARVGSGAVVEGFEPPVYIWEETIAPSGLAIYAGQQFPQWQGDLLHGGLA
                     AEGIVRTRIRNGEVVENELMLTELGRRIRDVQVDQEGAIWLVTDHEDGEVLRLVPEDT
                     VATGTIPRQQPPSATGTIPGTQQKE"
     gene            complement(2685..>2924)
                     /gene="prfC"
                     /locus_tag="VFX46_01645"
     CDS             complement(2685..>2924)
                     /gene="prfC"
                     /locus_tag="VFX46_01645"
                     /inference="COORDINATES: protein motif:HMM:NF027969.4"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEX5599320.1"
                     /translation="GTEYGLQVAFESSPYELVRWIESDDKAALQNFVNKNKAAIATDL
                     DGSQVFLAPSEFMLRWTAERAPEIKFTDIKMLIGG"
BASE COUNT          531 a          902 c          947 g          544 t
ORIGIN      
        1 tgctttcggc ggcgtggcag ggcagcacct tgccgcgcgg tgttacgttg agggactggc
       61 ggctccagcc gttcatgcag ggcttcggcc agcgcgagaa atgatcaggg gcgacgtagt
      121 cgatgagaat gccgggatcg agctcttgcc gcaggcgtgc cactgtggtg tgggccgttt
      181 cgacctgctc aatgctgggc agcaaagcgg cgcggtgtgt cagcgcccac ccatagtact
      241 gcgcgtgagc cacctcgatg cggcgggcac ctgcgcgatg agccgcaacg atcatgtcgc
      301 cgacgcgcgc aatgttccgc cggtgcacga cgacgttgat tgtcagcgga agtccgaggg
      361 caatgacggc gcggaccgtc ctttgtttcc acgcgtgagc gccggcgaga ccggcgattt
      421 catcggcgtt ggccgggtcc gtgtcctgcc aggagagctg gacgtgatcg agcccggctg
      481 cggccagctc ctcgagtcgc gcttcgctca aaccgacacc ggacgtaatc agattagtgt
      541 agagacctgc ccgcgatgca ccgcgggtaa gcgcgacgat gtcggcgcgg gccgtaggct
      601 cgccgcccga tagatggagc tgaagcacgc cgagcgctgc tgcctcatcg aagacacgca
      661 gccacgttgc cgaatcgagc tcgtcggagc ggcgatcaag ctcgactgga ttggagcaat
      721 aggggcacgc gagtgggcag cggtgtgtga gctcggccag aagccctaat gggagcggat
      781 gcgtcacagg tccaccaagc gccggtaggc gaggtcgagc agcagcttct cggtatcagc
      841 cgcaattcgg ccagggtcgg cctgatgaga acgggcgaga tcctcgacga ttgccgcaaa
      901 ggtcagcgta ccactgcagc gacgaaggat ctcaatggcc gacggattgg gggcgaccac
      961 gcgctccggc gcaagaagaa ccacgcgtcc acgtgcccgg tcttcatgca agcggacgcc
     1021 gcgtgccaag catggtcgcg cgtcgggcgc aatcctggct ctcatgtcgg tccagccgat
     1081 cgagacacgc gcttgtcgcg cgagccatgc cgctgcggaa ggccgagcca cctccgcaat
     1141 cgctctttgg tcaagcggcc gatgtcagct gtcgggccgc tctgaaggcc gtcggccctt
     1201 atttcgactc gtccggcgag tagtagccgg tcgcttcgag gccaatggcg gcgtcatgca
     1261 cagtcggttc cgtccagttc atggcctact ccttctgctg ggtgccaggg atcgtgcccg
     1321 tcgcggaggg cggttgctgc cggggaatgg tgcccgttgc gaccgtgtcc tccgggacga
     1381 ggcggagaac ctcgccatcc tcgtgatcgg tgacgagcca gattgcgcct tcctggtcga
     1441 cctggacgtc acggatgcgg cgaccgagct cggtgagcat gagctcgttc tccacgactt
     1501 cgccgtttct gatgcgcgtg cggacgatgc cttcggctgc gagcccgccg tgcagcaggt
     1561 caccctgcca ttgggggaac tgttggcctg cgtaaatggc caatccggag ggcgcaatcg
     1621 tctcctccca gatgtagacc ggcggctcga acccctcgac gacagcgccg cttcctacgc
     1681 gcgcgccgga gtaatcgacg ccgcccgtct ggaacggcca gccgtagttg cggcccggct
     1741 tgatgaggtt gatctcatcg ccgccgagcg gtccatgctc gttggcccac agctcgccgg
     1801 tttgaggatg gatggccata ccttggatgt tacgatgccc ccaggaatag atcatctcat
     1861 ctttatcggg atcgtttacg aacgggttgt ccggtggcgt ctgaccctca tcggttatgc
     1921 gcacgatcga cccggcatgg tctttcgggt cctgcgcgcg cgacatgttc cgccggtcgc
     1981 cgatcgagag atagatgctg ttgtcccggg gatcgaacac aaatcggccg ccgaagtgca
     2041 gaccgccgga atcgtgcttg ttcattgaaa aaatcagctc gacgtcctcg agcctgggcg
     2101 tgtcgccgct ctcgtcaagc cgcccgcgcg agatcgccgt acctgcgccc tcggaagagg
     2161 gctgagagta gctgaggtac acgaggcgat tttccgcgaa ctgcgggtgc agggcgacgt
     2221 ggaacagccc gccctgactg gagctcgtgt caccgatgta gcggaaggta tctggtacgc
     2281 cttccacggg cgcggaaagc tcacccttaa gattcccgat gcggaggtcg ccggaattgc
     2341 gctccgtgac gagataccgg ccgtccggaa gcagagcgat gccccacgga tggtgaagtc
     2401 cggtcgcgat cgtctccacc cgcacacgaa catgatcggt gtccacgatc tggtgatcct
     2461 gcgccagcac cgtatcggtc tggcctacca ggaccagcga aaacgagacg gcatgtgcgg
     2521 aaccgatcca acgacgcatt gggctccccg agacggcaga cggaaaaagt ctgcaatcgg
     2581 aaactcacca ctgcggcaat cggttccagg cttgtgcaac gaaagaagtt tagcccgctc
     2641 agctatcagc cgatttccaa atgttcctta gagccgcccg gttcttatcc gccgatcagc
     2701 atcttgatgt cagtgaattt gatctcgggc gcccgttccg cggtccaccg cagcatgaat
     2761 tccgacgggg caagaaaaac ctgcgagcca tcgagatccg ttgcgatagc ggccttgttt
     2821 ttgttaacga aattctgcag ggcagccttg tcgtcggact cgatccaacg caccagctcg
     2881 tacgggctcg actcgaacgc tacctgcagg ccgtattcgg tgcc
//
LOCUS       DASUMG010000102         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_656184, whole genome shotgun sequence.
ACCESSION   DASUMG010000102 DASUMG010000000
VERSION     DASUMG010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656184"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="VFX46_01650"
     CDS             complement(<1..1122)
                     /locus_tag="VFX46_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5599321.1"
                     /translation="MAIGGRALDLLMALVERAGEVVSHKELVKRAWPDVIVEETALRV
                     QIAALRRALGDGRDGVRYVTNVPGRGYCFVGPVQRSSPDGSQVVAPAVRIKDESLPER
                     LPRLVGRDETIDALRAEITFQRLVSIVGPGGVGKTTVAVAVAHEMSSEFRDAICFVDL
                     GAVKDGALVVPAVASAIGCLEQVQESLPRLLAFAADKQILLVLDSCEHVIDAVATLAE
                     GLCREAPLVHIVATSREALRVEGEHVYLLQALDTPSGEVNVTAASALASPAVQLFMDR
                     AATGGYRLGLSDEEAPIVSEICRQLDGMPLAIELTASRTSTYGINGLAQLIGDRLMLA
                     WQKRLGAPRHQTLQAMLDWSFDLLSDDEQAVLCALSVFAG"
     gene            complement(1320..2018)
                     /locus_tag="VFX46_01655"
     CDS             complement(1320..2018)
                     /locus_tag="VFX46_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5599322.1"
                     /translation="MIALETCEFETLLLAMAGHDLRQPLQMLQYVHDRFGNGARTRSE
                     LDMLRVGQSAIDRLREQLDQLLAALQLRERAGHIEASPVALEPLLREVYRESEWSALQ
                     KGIEIRLVPSAARIMSDPLLFRAVLRNLLNNAIKYTEPGGRILLGCRHFRDCIRIDVI
                     DTGVGISEEQLPRIFEAFARFDTSRNDGLGIGLFIVRQVVAMLGHRVDVCSVLSRGTR
                     FSILAKLAHGSEPS"
     gene            complement(2252..>2443)
                     /locus_tag="VFX46_01660"
     CDS             complement(2252..>2443)
                     /locus_tag="VFX46_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599323.1"
                     /translation="TFSTPAGTDREFFELHLAGWIGRFFADLERARPQSFYARVGALG
                     RLFVDVEREAFIAGPEKVV"
BASE COUNT          519 a          774 c          692 g          458 t
ORIGIN      
        1 gccagcgaag acagacagcg cgcatagcac tgcctgctcg tcgtccgaca acaaatcgaa
       61 actccagtcg agcattgcct ggagcgtctg gtggcgcggt gcacccaaac gcttctgcca
      121 ggcgagcatc aaccgatcgc cgatcagctg ggcgaggcca ttgatgccgt aggtgctggt
      181 cctgctcgcg gtcagttcga ttgcgagcgg cataccatcc agctggcggc agatctcgga
      241 gacaatcggc gcctcctcgt cggacagacc aagccggtat ccgccggtcg cggccctatc
      301 catgaacagc tgcacggcgg gtgatgccag cgcagaagcg gcggtgacgt tcacctctcc
      361 tgacggtgta tccagggcct gcagcaggta gacatgttcg ccttccacgc gcagcgcttc
      421 gcgcgaggtt gcgacgatgt ggacgagcgg cgcctcccgg cacagtccct ccgccagcgt
      481 agctaccgcg tcgatgacat gctcgcagct atcgagcacg agcagtattt gtttatcggc
      541 agcgaaagcc agcaatcgcg gcagcgattc ctgaacttgc tcgaggcaac cgattgccga
      601 tgccacggcc ggcacgacga gtgcaccatc cttgacggca ccgagatcga caaaacagat
      661 cgcatctcga aattcggaag acatctcgtg agcaacggcc acggcaaccg tcgtcttgcc
      721 gacgccgccc ggccccacaa tactcaccaa ccgttgaaag gtgatttccg cgcgcaatgc
      781 gtcgatggtc tcgtcacgac cgaccagtcg cggcaggcgt tcgggaaggg attcgtcctt
      841 gatgcggacg gccggagcca caacctgaga gccgtcaggc gacgaacgct gaaccggtcc
      901 gacaaagcaa taccccctgc caggcacgtt ggtgacatag cgcacaccgt cccggccatc
      961 gccaagcgcg cggcgaagcg cggcgatttg tacccgaagc gctgtttctt cgacgatgac
     1021 gtccggccat gctcgtttga ccaactcctt gtggctgacg acttcgcctg ctcgctctac
     1081 gagcgccatc aggaggtcga gcgcgcggcc accgatcgcc accggctcgt tttccttcaa
     1141 cagcaaccgc gcagaagcaa ccagacagaa cggcccgaag cgaaacgtct caagttcgtg
     1201 cgatcgtggc tgcacacctg acctgcaaag ctatctatcc atggaagacc cggtaccagg
     1261 atagcgcgct ttccggcaac gttgatgccg gggtgggtgc aaagcaggca tgccaaaggt
     1321 cacgagggct ccgagccgtg agccagcttg gcaagaattg agaatctcgt gccacgagac
     1381 agtaccgaac agacgtcgac gcggtggcca agcattgcga cgacttgtcg cacgatgaac
     1441 aggccaattc ccaatccatc gttccgcgag gtgtcaaaac gggcaaatgc ttcgaaaatt
     1501 ctcggcagct gttcctcgga gatgccgacg ccggtgtcga ttacgtcgat ccggatgcaa
     1561 tcccgaaagt gccggcatcc cagaagaatc cggccccctg gctcggtata tttgatggcg
     1621 ttgttgagca agttgcgaag caccgcacgg aagagcaatg gatcgctcat gatccgggcg
     1681 gctgatggca ccaggcggat ctcgatccct ttctgaaggg cactccattc gctttcccgg
     1741 taaacctctc gcagcagtgg ctccagagcc accggcgacg cctcgatatg tccggcacgt
     1801 tctcgcagct ggagcgcagc caacaattgg tcaagctgct cccttaaccg gtcgatggcg
     1861 ctctgaccca ctctcagcat atccaattct gaccttgtcc gagccccatt gccgaaccgg
     1921 tcgtgaacgt actgaagcat ctggaggggt tgccggagat cgtggcctgc catggcgagg
     1981 agaagtgttt cgaactcgca agtctccaat gcgatcactt tgcactccat acgaccggcc
     2041 tgagggcatt aacgcgttca ccatgactgc ttgctctacg catcaaacga attcctttcg
     2101 acggataggc tgttcttgcc cgataaatgt cttgcttacg gaagcgcgtc atattgagcg
     2161 agttcggcga tgtgaatctc gccctcctcc cgctagctgc cgatactccc tttcttgttc
     2221 tgcttccttg atcgcccgca gcgaatccac ctcagaccac cttctccgga ccagcgatga
     2281 aggcttctct ctcgacgtcc acgaacagtc gcccaagtgc gcccacgcgg gcatagaacg
     2341 attgcggcct ggcccgctca agatcggcaa agaagcgtcc gatccagcca gcgagatgca
     2401 attcaaagaa ctcacggtcg gtgccagctg gcgttgagaa ggt
//
LOCUS       DASUMG010000103         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_174459, whole genome shotgun sequence.
ACCESSION   DASUMG010000103 DASUMG010000000
VERSION     DASUMG010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174459"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VFX46_01665"
     CDS             <1..290
                     /locus_tag="VFX46_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008134529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX5599324.1"
                     /translation="ELGQDISGHKPTTFEELEDWEGLNFDLIITLSPEAHHKALELTR
                     TMAADVEYWPTSDPTGMEGNREQKLAASREVCDGLFMRIRRRFAKAGAASG"
     gene            539..1162
                     /locus_tag="VFX46_01670"
     CDS             539..1162
                     /locus_tag="VFX46_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf-like protein"
                     /protein_id="HEX5599325.1"
                     /translation="MLGRPKIVLASGSPRRLSLLNQAGIEPDALRPADVDETPKRGEL
                     PRACANRLARAKADAALKSVQLDDELRGSFILAADTVVAVGRRILPKANLVDEAAQCL
                     RLLSGRNHRVYTAICLVTPKEAFRQRLIETRVRFKRLSEDDIQAYIGSGEWRGKAGGY
                     AVQGIAGSFVVKMVGSYTNVVGLPLYESVTLLGGEGFPIRFGWLNAS"
     gene            1227..1379
                     /gene="yacG"
                     /locus_tag="VFX46_01675"
                     /pseudo
     CDS             1227..1379
                     /gene="yacG"
                     /locus_tag="VFX46_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084078.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
     gene            1521..1596
                     /locus_tag="VFX46_01680"
     tRNA            1521..1596
                     /locus_tag="VFX46_01680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Phe,seq:gaa)
     gene            1737..>2063
                     /locus_tag="VFX46_01685"
     CDS             1737..>2063
                     /locus_tag="VFX46_01685"
                     /inference="COORDINATES: protein motif:HMM:NF040136.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5659 domain-containing protein"
                     /protein_id="HEX5599326.1"
                     /translation="MKYDTHNIDEAAYLALQGHRFTVTRTGPISALFSFETDEHFDEA
                     LAKFWRGEATVQLHRWLATRAALKNECTGQPLLAKRVPSPPLAPAEPETKTAVRTGDA
                     YWYRDGD"
BASE COUNT          371 a          701 c          624 g          367 t
ORIGIN      
        1 ccgaactcgg ccaggatatt tccggccaca agccaacgac gttcgaagag ctcgaggatt
       61 gggaagggct caatttcgat ctcatcatca cgctgtcgcc ggaagcccat cacaaggcgc
      121 tggagctgac gcgcaccatg gccgccgatg tcgaatactg gccgacgtcc gatccgaccg
      181 gcatggaagg caaccgcgag cagaagctcg ccgcctctcg cgaagtctgc gacggccttt
      241 tcatgcgcat ccgccggcgc tttgccaagg caggcgcggc gagtgggtag atcgggttct
      301 tccgtcgccc ccgcgaacgc ggagccctat acgccgcgac cgtgcgattt taggcactgc
      361 cagtcgcccc tgtcatttcc ccgacgacct cctgtggtta tgggtccctg ctttcgcagg
      421 gacgacggaa gatcggtaat ccgctcaacc acttgttccc cggttgtgga atggagtgcc
      481 ttccgatagg ttccgcgcgc gcgtcccatc cgaatccttt tcccgcgaca aatcccgcat
      541 gcttggccgt cccaaaatcg ttctcgcttc cggttcgccg cgacggctca gcctgcttaa
      601 ccaggccggc atcgagcccg acgcgctgcg cccggccgat gtcgacgaaa cgcctaagcg
      661 gggcgagctg ccgcgcgcct gcgccaatcg tctcgcccgc gccaaggccg acgcagcgct
      721 gaaatccgtg cagctcgacg acgagctgcg cggttccttc atcctcgcgg ccgatacggt
      781 ggtcgcggtc ggtcgccgca tcctgcccaa ggccaacctg gtggatgagg ccgcgcagtg
      841 cctgcggctg ctctcggggc gcaatcaccg cgtctacacg gcgatctgcc tggtgacgcc
      901 aaaggaggcg ttccgccagc gcctgatcga gacgagagtc cgtttcaagc ggctcagcga
      961 ggacgacatc caggcctata tcggttccgg cgaatggcgc ggcaaagccg gcggctacgc
     1021 cgtgcagggc atcgccggat cgttcgtggt caagatggtc ggctcctaca ccaacgttgt
     1081 cggcctgccg ctctacgaat cggtcacgct gctgggcggc gagggctttc cgatccgctt
     1141 cggctggttg aatgccagct aagccgcccg agggcgccgg tgggcccaag gatccaggcg
     1201 gggccaagga cgctggcgga aagccgccgc caaaaccctg cccggtctgc ggcaagccgg
     1261 ccgaccctgc caggctcccg ttctgttcca gccgctgccg cgacgtcgac ctgaaccgct
     1321 ggctgtccgg ctcctacgtc atccccggcc gcgacaccga cgcggaagac gccgaataac
     1381 ggctattccc caataacttg gggagagagc acgaagcggg cccgccacgc cgaagccgca
     1441 ggcgaaggcg ggtggacatg accgcctgac ctctctataa accgcccgct cgctacggcc
     1501 tttgggccct cgccgagtat gcccaggtag ctcagttggt agagcatgcg actgaaaatc
     1561 gcagtgtcgg tggttcgatc ccgcccctgg gcaccatcat gcctaatacc acttcatccc
     1621 aacagcccgc caggcgaccg ctcggcgtag ccggctatta accacgctgg cggcgcacgg
     1681 ctttttgttt cactcaatgc gtgcattctg gtctcacctc gtgccccggg atgcaaatga
     1741 agtacgacac gcacaacatc gacgaagccg cttaccttgc gctgcagggc catcgcttca
     1801 ccgtcacgcg cacgggaccg atctctgcgc tgttctcgtt tgagacggac gagcacttcg
     1861 acgaagccct tgcaaagttt tggagaggcg aggcgactgt gcagcttcac cgttggctcg
     1921 ccacgcgagc tgctttgaaa aacgaatgca cgggccagcc gcttctggca aagcgtgttc
     1981 cttcaccgcc gcttgctcct gcggaacctg agacaaagac tgccgtaagg acaggcgacg
     2041 cttactggta ccgtgacggg gat
//
LOCUS       DASUMG010000104         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_13723, whole genome shotgun sequence.
ACCESSION   DASUMG010000104 DASUMG010000000
VERSION     DASUMG010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13723"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFX46_01690"
     CDS             <1..330
                     /locus_tag="VFX46_01690"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5599327.1"
                     /translation="VSLALQLCLEEAVANIIMYGAIDGGLEIAVELERRGQTLIARIE
                     DNGLPFDPTQKPPPRPATTLKDAKIGNLGIYLARCFASHMDYERRQGRNRLTLRFDEP
                     QAASPQP"
     gene            340..>2360
                     /locus_tag="VFX46_01695"
     CDS             340..>2360
                     /locus_tag="VFX46_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX5599328.1"
                     /translation="MPEAFVFGPFRLLITRRELMAHGVPVPVGQRAFEILLLLVSRHG
                     ELVTKDELMAAVWPGIVVEENNIQVHVSALRKVLAKAGDSRRYLVTVPGRGYRFVAPV
                     ECDSANQPNRPTTAVPGQAATVAAAGSPATRNNLPHPLTSLIGREAEVAEIKAALASH
                     RLVTLTGSGGVGKTRLVIDVGRSVLDRYPDGVWLAELAPLTDSQLVTFTIGAVLGVSL
                     SSAATATEALASALHTKQLLLIIDNCEHVIAEVARVAEALIQACAHLSIVASSRERLA
                     IAGESVIRVPSLPTPQASAGLTAMAACEYPAVRLFVERASALGLGFALTDANAATIGS
                     ICTRLDGIPLAIELAVPRLRVLSVEQLARGLEERFRLLTGGNRTALPRQQTLHALIDW
                     SYALLNDPEKLLLARLAVFSGSALLTSITAVVVSVEIPPEQCGDLLYSLTEKSLMHVD
                     PIAGETRYRLLESTRYYAAEKLGAAPEIRRRHAAHFLARFAEATAEWETTPTLRWIVQ
                     YEADLDNLRGALEWAFGKDGDVNIALDLVGRSHVIWAELGLMLEHRRWVNEALAKIRK
                     ETPAEVTARLLSWQAGDVRELDDPTDYDEAMRAAALYREVGDGFQEGRVLLRAGTARL
                     SPDSVAEGENLLRKAQALVRPFGPTKTLARCLSAQASARLFAGDIGKAR"
BASE COUNT          449 a          707 c          752 g          452 t
ORIGIN      
        1 gtctcgctcg ctcttcaact gtgcctcgaa gaggcagtcg ccaatatcat catgtatgga
       61 gctatcgatg gtgggctgga gattgcggtc gagttggagc ggcgcggcca aacgctaatc
      121 gcccgaatcg aagataacgg cctgcccttc gatcccacac aaaagccacc acctcggcct
      181 gcgaccacgc tcaaggacgc gaagatcggc aatctcggga tctatcttgc gcgctgtttt
      241 gccagtcata tggattacga acgtcgacaa ggtcgcaatc ggctgaccct gcggttcgac
      301 gagccgcagg cagcgtcacc gcagccttag tgaacctcga tgccggaggc attcgtattc
      361 ggaccattcc gtctgctgat cactcggcgc gagctcatgg cccacggcgt gcccgttcca
      421 gtcggccagc gcgccttcga aatattgctg ctgctggtga gccgccacgg ggagctggtg
      481 acgaaggacg aactcatggc agcggtctgg ccaggtatcg ttgttgaaga aaataacatt
      541 caggtgcacg tctcggcctt gcggaaggtt ctggccaagg cgggcgattc ccggcgctac
      601 ctggtgacgg tacctgggcg gggctatcgc ttcgtggcac ccgtggaatg cgacagcgcg
      661 aaccaaccca acagaccgac gacagccgtg cctggccaag cagcgacggt ggcagctgcg
      721 ggaagtccgg cgacccgcaa caatcttcca cacccgttga ccagtttgat cggacgcgag
      781 gccgaggttg cagagataaa ggcagctctc gccagccatc gcctcgtgac gctgacggga
      841 tcgggaggcg tcggcaagac gcggctcgtc atcgatgtgg gtcgaagcgt cctcgaccgt
      901 tatccggacg gcgtgtggct tgcagagctc gctccgctca ccgattcgca gttggtaacc
      961 ttcaccatcg gtgccgtatt gggtgtcagc ctgagcagtg cggctaccgc gacggaggcc
     1021 ttggcttcgg cactccacac caagcagctc ctgttgatca ttgataattg cgagcatgtc
     1081 atcgcggaag tcgcccgcgt cgcagaggca ctgatccagg cctgtgcgca tttatcgatc
     1141 gtggcatcga gccgagagcg gctggcgatc gcaggcgaaa gcgtaattcg cgtgccgtca
     1201 ttgccaacgc cgcaagcgag tgccggtctg accgctatgg ccgcgtgcga atacccagcc
     1261 gtgcggttat tcgtcgagcg tgcgagcgcc cttgggctgg gattcgctct gaccgacgcc
     1321 aacgcggcca caatcggctc gatctgcacc cggctcgatg gaattccact ggcgatcgaa
     1381 ttggcggtgc cgcgactaag ggttctatct gttgaacagc tagctcgcgg gctcgaggag
     1441 cgcttccggc tcttaaccgg aggcaaccgc acggccttgc cgcgacagca gacactgcat
     1501 gccctgatcg actggagcta cgcgctcttg aacgatccgg aaaagctctt gctcgcccga
     1561 ttggcggtct tttcgggtag cgccctgctg acgtcaatca cagcggtcgt tgtcagcgtc
     1621 gaaatcccgc cggagcagtg tggggacctc ctgtattcgt tgactgagaa gtccttgatg
     1681 catgtcgatc caatcgccgg agagacccgc taccgcctgc tcgaatcaac gcggtattat
     1741 gcagccgaaa agcttggcgc tgcaccggag atacggcggc ggcacgccgc ccacttcctc
     1801 gcccggttcg cagaggcgac ggcagagtgg gaaacgaccc cgacgctgcg atggatcgta
     1861 caatatgaag ccgacctcga caatttgcgc ggtgcattgg aatgggcttt cgggaaggac
     1921 ggcgatgtta acatcgcgct cgatctcgtg ggccgcagcc acgttatatg ggccgaactc
     1981 gggctgatgc tggagcaccg gcgctgggtc aacgaagcgc tcgccaagat ccgcaaggag
     2041 acacctgcgg aagtgacggc ccggctgtta tcttggcagg cgggcgatgt tcgggagctc
     2101 gatgatccta ccgattatga cgaggctatg cgtgcagctg ccctttatcg agaggtcggc
     2161 gacggctttc aggagggtcg cgtgctgttg cgcgcaggca cggcgcggct atcgccggac
     2221 agtgtggcgg agggcgaaaa tttgctgcgt aaagcgcaag cgcttgttcg cccgttcggc
     2281 cctacgaaga cgttggcgcg atgcctaagc gcccaggcct ccgctcgcct gttcgccggc
     2341 gatataggca aggcgcggat
//
LOCUS       DASUMG010000105         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_94251, whole genome shotgun sequence.
ACCESSION   DASUMG010000105 DASUMG010000000
VERSION     DASUMG010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94251"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..269
                     /gene="hfq"
                     /locus_tag="VFX46_01700"
     CDS             18..269
                     /gene="hfq"
                     /locus_tag="VFX46_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEX5599329.1"
                     /translation="MAERAQSLQDTFLNHVRKNKTPLTIFLVNGVKLQGIVTWFDNFC
                     VLLRRDGHSQLVYKHAISTIMPGQPIHLMEQDPAEAQQG"
     gene            409..1701
                     /gene="hflX"
                     /locus_tag="VFX46_01705"
     CDS             409..1701
                     /gene="hflX"
                     /locus_tag="VFX46_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX5599330.1"
                     /translation="MRRGNGPRGATLHSPEDRLTEAVGLAEAIDLEVCAKLVVSIARP
                     QPATLFGSGKVEELKELVARHEADLVVVDHAVTPIQQRNLERAWNTKVLDRTGLILEI
                     FGRRARTREGRLQVELAHLTYQKSRLVRTWTHLERQRGGYGFLGGPGETQIETDRRLI
                     QERIDAIRKELDTVVRTRELHRSGRRRVPYPLVAIVGYTNAGKSTLFNKLTGAGVVAM
                     DQVFATLDPTMRELRLPSGRRIILSDTVGFISDLPTTLVAAFRATLEEVVEANLILHV
                     RDISHPEAQAQREDVESVLAELGIDANQPDGRILEVWNKIDLVPPERLEELQHASRRA
                     PHAPLLVSATEAIGLDELLQAIDRELGRADEELTLTVPATAGGMLAWLHENAQVLSQE
                     TLDSGEIICHVRIAREKRGRLFSRLHKYGLSITARAAS"
     gene            complement(1749..>2445)
                     /locus_tag="VFX46_01710"
     CDS             complement(1749..>2445)
                     /locus_tag="VFX46_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599331.1"
                     /translation="VALLGLAAGAGIAAALPQLPGSTKLSLGERLAPLGDKRVAMILM
                     TTLLVLGGLYTVYSYVSVVFDRATAGNGSVLALLIGIWGMAATIGNLVAGSLTDRFGS
                     RAVINLAIAVVAIDFALIPLSSATFTGSVVALIVWGLCGWGMLVPQQHRLVSISPALA
                     PILLALNAATIYIAVSLSGLLGAAALQVMQPYNLALVGAVLIIFGLGSAELAYMLMAQ
                     QQGVSRQSKVKKF"
BASE COUNT          515 a          737 c          774 g          419 t
ORIGIN      
        1 aaaacggata agaagaaatg gctgaacgag cacaaagcct gcaggacacg tttctgaatc
       61 atgttcggaa gaacaagacg ccgctcacga tcttcctggt aaacggcgtg aagctccagg
      121 gcatcgtcac gtggttcgac aacttctgtg tcctgctgcg gcgtgatgga cactcgcaac
      181 tggtctacaa gcacgccata tcgacaatca tgccgggtca acctatccat ctaatggaac
      241 aggatccggc tgaggcacag caggggtagg ttctcgatta cgcgcgcaga gaagaccaga
      301 cggcgctatg agagccgggg cgacgatggc ttcggcaaga aggacctaac gtgtgccatc
      361 gttgttgtcc cggtcctgca tctcaatggc aagaatggga cgctcgccgt gcggcgggga
      421 aatggcccgc gcggcgccac actgcattca cctgaggatc ggctgacaga ggcggtgggc
      481 cttgccgagg ccatcgatct tgaggtctgc gccaagctgg ttgtgtccat tgcgcggccg
      541 cagccggcga ccctgttcgg ctccggcaag gtcgaggagc tgaaagagct tgtcgcccgg
      601 catgaagcgg acctcgtcgt agtcgatcac gccgtcacgc ccatccagca gcgcaacctg
      661 gagcgagcct ggaacacgaa ggtgctcgat cgcaccggcc ttatcctgga gatcttcggg
      721 cgccgggccc ggacgcggga aggtcggctc caagtcgagc tggctcacct cacttatcaa
      781 aagagccggc tggttcggac gtggacccac cttgaacgtc agcgcggcgg ttacggcttc
      841 cttggcggcc cgggcgaaac gcagatcgag acggaccgcc gcctcatcca ggagcgtatc
      901 gacgccatcc gcaaggagct ggatacggtg gtgcgcacgc gcgagctgca tcgttccggg
      961 cgccgccgcg tgccttatcc gctcgtcgcc atcgtcggct acacgaacgc cggcaagtcc
     1021 acgctcttca acaagctaac gggagcgggc gtcgttgcca tggaccaggt gttcgcgacg
     1081 ctcgatccaa caatgcgcga gctgcgcctg ccgagcggtc ggcggatcat tttgtctgac
     1141 accgtcgggt tcatctcgga tttgccgacc acgctcgtcg ctgcgttccg tgccacgctc
     1201 gaggaggtgg tagaggcgaa cctcatcctg cacgtccgcg atatcagcca tccggaagcg
     1261 caggcccagc gcgaggacgt cgagagtgtc cttgccgagc ttgggatcga cgcgaaccag
     1321 ccggacggaa ggattctgga ggtatggaac aagatcgatc tcgtcccgcc ggaacggcta
     1381 gaagagcttc agcatgcgtc ccggcgggcc ccacacgcac ccctattggt ttccgccaca
     1441 gaggcaatcg gcctggacga gcttctgcag gcgattgatc gggagcttgg cagagcggat
     1501 gaggagctga cgctgaccgt acccgcgacc gcgggcggca tgctggcgtg gctgcacgag
     1561 aacgcgcagg tcctatcgca ggagacgcta gattccggcg agatcatctg ccatgtccgc
     1621 atcgcccgcg aaaagagggg gcgtctcttc agccgactgc acaagtatgg cctgtcgata
     1681 acggcccgcg ccgcgagttg acgccgctgt tgggcgctga aggcgcaact tcggtgacgt
     1741 tcaagccgtc agaacttttt gacttttgac tgccgggaca caccttgctg ctgtgccatc
     1801 agcatgtacg cgagctcggc tgagcctagg ccgaaaatga tcagaactgc tccgactagc
     1861 gccaaattgt agggttgcat tacctgaagc gcagcggctc ccaataagcc ggaaagggag
     1921 accgcgatgt agatggtggc ggcgttcagc gcgagcagga ttggtgcaag cgccggtgaa
     1981 atgctgacga gccggtgctg ctgtgggacc agcatacccc atccgcacag accccagacg
     2041 atgagagcaa cgacgctgcc ggtgaaagtc gcgctcgaca ggggtatcag agcaaaatca
     2101 attgcgacca cagcaatcgc gagattgatt acagcacggc taccgaaccg atccgtaagg
     2161 cttccggcca cgaggttgcc gatggtcgct gccatgcccc agatgccgat caggagcgcc
     2221 aaaacgctac cgtttccagc ggtcgcacgg tcgaagacca cgctcacata gctgtatacg
     2281 gtatagagac cgccgagcac gagcagcgtg gtcatcaata tcatagcgac gcgcttgtca
     2341 ccgagtggag cgagacgctc gccgaggctg agctttgttg agcccggcaa ttgcgggaga
     2401 gccgccgcaa tcccggcgcc agcggccaaa cccaaaagcg cgacg
//
LOCUS       DASUMG010000106         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_13775, whole genome shotgun sequence.
ACCESSION   DASUMG010000106 DASUMG010000000
VERSION     DASUMG010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13775"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1045)
                     /locus_tag="VFX46_01715"
     CDS             complement(119..1045)
                     /locus_tag="VFX46_01715"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEX5599332.1"
                     /translation="MKGQRNFSGVIAPVLTPFGEDGAPDPDRFIEHVEWLLEDGCTAL
                     APFGTTSEANSLGLDERMELLEELVDSGVDPSKLMPGTGSCSVADAAILTQHAVDLGC
                     GGVLMLPPFYYKNPSEEGLFRFFAEVIDDVGDDRLRVYLYHIPPVAQVGFSLKLIERL
                     KNEFPGIVVGLKDSSGDWSNMKAILDAFPDFELFPGSELYLLEGLRNGAAGVISATAN
                     VSARAMRRLYDDWRGPNADALQEEISALRKTIQSFPVIPTLKALIAHYRNDQGWAEVR
                     PPFTPLPAAEAERAIKMLADQHNFRLEFAEAA"
     gene            complement(1108..1740)
                     /locus_tag="VFX46_01720"
     CDS             complement(1108..1740)
                     /locus_tag="VFX46_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007759752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5599333.1"
                     /translation="MSIDLYTWSTPNGRKVSIMLEEVGLPYTVHPINIGKDEQFDPAF
                     LKISPNNRIPAIVDRDNGLSLFESGAILMYLAEKTGKLWPKEGEAHWRTVEWLMWQMG
                     GVGPMLGQVHHFVKYNKGKAPYAEERYTKEALRLYGVLDRRLAEVEYVAGDYSIADIA
                     IWPWISRFEWQEIDLNKFPNVLRWYRAIAGRPAVQKGYHVPVPQPSIPMP"
     gene            complement(1824..>2033)
                     /locus_tag="VFX46_01725"
     CDS             complement(1824..>2033)
                     /locus_tag="VFX46_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX5599334.1"
                     /translation="SWWICHAMGLPDDITAVIVVAAATPVGVLITIFAAEYKTEAEFI
                     STAVVISTALSPIFVTGWILATRVF"
BASE COUNT          410 a          650 c          582 g          391 t
ORIGIN      
        1 cagttggcag cgccagggcc gagcagaagc gcaacgactt ccttgatgac tctccccccc
       61 tccagcctcc ccctcctcaa accgaggagg gggttttctc gttcagccgc ggctagggtc
      121 aggcagcttc agcgaattcg agccggaaat tgtgctggtc cgcgagcatc ttgatggccc
      181 gctcggcctc cgccgcggga agcggcgtga atgggggacg cacctctgcc cagccctggt
      241 cattgcgata gtgcgcaata agcgccttga gagtcggaat gacggggaag ctctggatgg
      301 tcttccgcag cgccgagatt tcttcctgca gggcgtcggc attcggcccg cgccagtcgt
      361 cgtagagccg tctcatggcg cgtgcgctaa cattggccgt ggcagagatc acaccagcgg
      421 ccccgttgcg caggccctcg agcagataga gctccgaacc cgggaacagc tcgaagtccg
      481 ggaaggcgtc gagaatggcc ttcatattgg accagtcgcc tgaggaatcc ttcagtccta
      541 ctacgatgcc tgggaactcg ttcttcaacc gctcgatgag cttcaacgag aaaccgacct
      601 gggcaaccgg cggaatatga tagaggtaga cccgcagccg gtcatcgccg acatcgtcga
      661 tcacctcagc gaagaaccgg aaaagcccct cctcgcttgg attcttgtag tagaacggcg
      721 gcagcatcag caccccgcca cagccgagat ccaccgcgtg ctgtgtgaga atggccgcat
      781 cggctaccga gcacgagccc gttccgggca ttagcttgga cggatcgacg ccgctgtcga
      841 ccagctcctc gagcagctcc atgcgctcgt cgaggccgag cgagttcgcc tcgctcgtcg
      901 tcccgaacgg cgccagcgcc gtgcagccat cctcgagcaa ccattcaacg tgctcgatga
      961 agcggtccgg atccggtgcg ccatcttcgc cgaacggcgt cagcaccggc gcgatcacac
     1021 cggaaaaatt gcgttggccc ttcatgttgc ctgttccttc atgaacataa acctaggtgt
     1081 tcctgggtgc ccctatccca cgggatgtca tggcatggga atggatggct gcggcaccgg
     1141 cacgtgatac cccttctgca ccgcaggccg tccggcgata gcacggtacc agcgtagcac
     1201 gttgggaaac ttgttgaggt cgatctcctg ccactcgaac cgtgagatcc acggccaaat
     1261 cgcgatatcc gcaatggagt agtccccggc cacgtactca acctcagcca accggcgatc
     1321 gagcacgccg tagaggcgca gggcttcctt cgtgtagcgc tcctccgcat agggcgcctt
     1381 gcccttattg tatttcacga agtggtgcac ctgcccgagc atcggcccca caccgcccat
     1441 ttgccacatc agccactcga ccgtacgcca gtgggcctca ccctccttcg gccagagctt
     1501 acctgtcttc tcggcaagat acatgaggat ggccccggac tcgaaaaggg acaatccatt
     1561 gtcgcgatcg acgatggcag gaattctatt gttggggctg attttcagga acgctgggtc
     1621 gaactgctcg tctttcccga tgttgattgg atgaaccgtg tacgggagac caacctcctc
     1681 cagcatgata gagaccttac gcccgttcgg cgtagaccag gtgtaaagat cgatcgacat
     1741 gagtcagtcc tcccgcgccc tcttacgcaa ccgccggagc ctagacgaaa ccgccctcgt
     1801 gctgcaatgc cttcaggagc gcgtcaaaaa acccgtgtag cgagaatcca tccggttacg
     1861 aagatcggtg aaagtgccgt cgatattacg acggccgtcg agatgaactc cgcttccgtc
     1921 ttgtactcgg ccgcgaagat ggtgatcagt acgcccacgg gggttgctgc cgcgacgacg
     1981 atgacagccg tgatatcgtc cggcaatccc attgcatggc agatccacca gct
//
LOCUS       DASUMG010000107         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_978137, whole genome shotgun sequence.
ACCESSION   DASUMG010000107 DASUMG010000000
VERSION     DASUMG010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_978137"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VFX46_01730"
     CDS             complement(<1..448)
                     /locus_tag="VFX46_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599335.1"
                     /translation="MIVRNRRLWIGIGTATLAGAVASGAVAQEAKHPGHKEQANTSQP
                     GSPNMLAAPQAGEAYLTDGGPRDTRIRIYRDIALMRGHLRVGDELIREGRWEDALVHF
                     LHPTEELYGAMERYIKLHGVTPFDRELKAQAEAVKAKRMNAYEQNKK"
     gene            533..1192
                     /locus_tag="VFX46_01735"
     CDS             533..1192
                     /locus_tag="VFX46_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEX5599336.1"
                     /translation="MMLSIEGVLTPAEVADVVAALGRARFSHGRATAGWAARSVKNNG
                     QARESDEIRTLRELISERLLSHPVFEMAARPKRIIGPMFSRYRNGDTYGEHVDEPLMD
                     GARTDVSFTLFLTAPESYVGGELIISSPAGEDAIKLPAGAVIVYPATTLHRVAPVTSG
                     ERLAAVGWVRSYIRNAEQREILFELDTARRDVFQREGASTTFNQLSKAVVNLIRMWCD
                     D"
     gene            complement(1201..1707)
                     /gene="bfr"
                     /locus_tag="VFX46_01740"
     CDS             complement(1201..1707)
                     /gene="bfr"
                     /locus_tag="VFX46_01740"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX5599337.1"
                     /translation="MKGKQEVLEVLNEALRLELAAINQYWLHYRLTEDWGYTLLAKKE
                     REESMDEMMHADKLVQRIIFLEGHPNMQTIAPLRIGQNVREVLEADLAAEYEARDHYI
                     HGRKVCEAAEDYVSMEIFEELLEDEEGHIDFLETQLDLLNKIGEQNYGQLQAASADKV
                     NSDNGNDD"
     gene            complement(2223..2720)
                     /locus_tag="VFX46_01745"
     CDS             complement(2223..2720)
                     /locus_tag="VFX46_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX5599338.1"
                     /translation="MRLTKQTSHAIRILIHCARARGQLIKVAEIARELDLTQQNVFKI
                     VHLLTRSGFLTAVRGRYGGVRLAREPKDIRIGDVVRATEVTHVAIEGEDVPTRPGQSQ
                     PISQVLDDALEAFISVLDQHTLEEMARAPRQAISSEDEHVPAKGRTRPRKTKAAARPA
                     VGPGR"
     gene            complement(2829..>3182)
                     /locus_tag="VFX46_01750"
     CDS             complement(2829..>3182)
                     /locus_tag="VFX46_01750"
                     /inference="COORDINATES: protein motif:HMM:NF023302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3394 domain-containing protein"
                     /protein_id="HEX5599339.1"
                     /translation="PANERLNFVVSGFNIRGDEITKTVNIPLGPVAPAPQRLRAIGLG
                     VTPLGDTVSITNVQFGSYAKRIGLEVGYEVVAVLEPADRPSHIWPIGAGFLAIGGIAL
                     IQRMRVARAPEAQPA"
BASE COUNT          574 a          991 c          939 g          678 t
ORIGIN      
        1 ccttcttgtt ctgctcgtag gcattcatgc gcttcgcctt gaccgcctct gcctgcgcct
       61 tcaattcgcg gtcgaagggc gtgacaccgt ggagctttat gtagcgctcc attgcgccat
      121 aaagctcttc agtcggatga agaaagtgaa ccagcgcatc ttcccagcgg ccttcgcgga
      181 tcagctcatc gccaacgcgc aaatgtccgc gcatcagcgc gatgtcccgg tagattcgga
      241 tgcgcgtgtc tcttggcccg ccgtccgtaa gataagcctc tccggcctgt ggcgccgcga
      301 gcatgttggg actgcccggc tgggaggtgt tcgcttgctc cttgtgcccc ggatgcttgg
      361 cttcctgagc aacggcgccg gaagcgactg ctcccgccaa ggtcgccgtg ccgatgccaa
      421 tccagaggcg gcgattgcga acgatcatgc tgatcctccc gttgtggtgg catcattatt
      481 gacatttttg gtagagaaac aagagcaccg gcagtcgtag ggaaatgcgc agatgatgtt
      541 gtcgatcgaa ggtgtgctga ccccagcgga ggtcgcggat gtcgtagcgg cgcttggtcg
      601 cgcgcgtttc tcacacggac gggcaactgc cggttgggcc gcacgatcag tgaagaacaa
      661 tgggcaggcc cgggaaagcg atgagatccg gacgttgcgc gagctgatct ccgagcggct
      721 tctgagccat cccgttttcg aaatggccgc ccggccgaag aggatcatcg ggcctatgtt
      781 ctcgcgctac cggaatggcg acacctacgg cgagcacgtt gacgagccct tgatggatgg
      841 ggcccgcacg gacgtttcct tcaccctttt cttgacggct ccggagagct atgtcggcgg
      901 tgagctgatc atctcgtccc cggctggcga ggatgcgatc aagcttcccg ccggcgccgt
      961 catcgtttac cccgcgacga cccttcatcg cgtggcacct gtgacgagcg gggagcgcct
     1021 tgcagcggtc ggctgggtgc gcagctacat caggaacgcg gagcagcgcg agatcttgtt
     1081 cgagctcgac accgctcggc gtgacgtttt ccagcgtgaa ggagcgtcta ccaccttcaa
     1141 tcagctgtcc aaagcggtcg tgaaccttat tcggatgtgg tgcgatgact gagagcaggg
     1201 ttagtcgtcg ttcccgttgt cggagttaac cttatccgcg gatgcggcct ggagctggcc
     1261 gtagttctgc tcgccgatct tgttgaggag gtcgagctgc gtctcgagga aatcgatgtg
     1321 cccttcctcg tcctccagca gctcctcgaa gatctccatg gagacgtagt cctcggccgc
     1381 ctcgcaaacc ttgcgcccat gaatgtagtg atcccgcgcc tcgtactctg ccgccaggtc
     1441 agcctccagg acttcccgca cattctgccc gatccgcagc ggcgcgatgg tctgcatatt
     1501 gggatgccct tcgaggaaga tgatgcgctg cacgagcttg tcggcgtgca tcatctcatc
     1561 catggactcc tcgcgctcct tcttggcaag cagcgtgtac ccccagtcct ccgtcaaacg
     1621 ataatggagc caatactggt taatggcggc cagctcgaga cgcagcgcct cgttcagcac
     1681 ttcgaggact tcctgcttgc ccttcatatc gctcctcctc ttccggacac tgtttgtcca
     1741 gataggatga gaatcactct gctgcaatca gttgagcccg ccgtcggttc cgtgccgcac
     1801 gcctctggtc caatcgtatc agtctgcttt tcgtgattgc gcacgcgtcc gccgccaacc
     1861 ggccttcccg ctcgagcttc tccacgatcg cgtagatcgt cgaaacagcg agcggagcgc
     1921 agctgcagca cgccatggtc ttcgacatgt gccggtaaac gacacccggt gtcggaagag
     1981 gggcgttcgg ttgagagagg atctcgatca gagccttttc aatgtcccga tccgagatga
     2041 ccgtacagga gcagataatc acgatttcta ctctcggctg gcttcttcag cagcccgcgt
     2101 gggacacacc gatctcgcct tcaccataca agccaaccct tccttatcat aatactgggg
     2161 gccttgaggc gcgtcaaaat gggagatgat tgctcctcag ccagcgacca ggagcatgcc
     2221 tgctatcgac ctggtccgac tgcgggccga gcggcagctt tcgtctttct gggcctcgtc
     2281 cgccctttgg ctggcacgtg ctcgtcttcg ctagaaatcg cttgccgcgg cgcgcgcgcc
     2341 atctcctcga gcgtgtgctg atccaatacg gaaatgaaag cctcgagggc atcgtcaagc
     2401 acctggctga tcggctggct ttgcccaggg cgcgttggca catcctcccc ttcgattgcg
     2461 acgtgggtga cctcggtcgc acgcacgaca tcccctatac ggatatcctt cggctcccgc
     2521 gccagccgca cgcccccgta acgcccgcga accgccgtca ggaagccaga ccgcgtgagc
     2581 aggtgaacga tcttgaacac gttctgctgt gtcagatcga gctccctggc gatctccgcc
     2641 accttgatga gctgcccacg cgcccgggcg caatgaataa ggatccggat agcgtggctt
     2701 gtctgcttgg tcagtcgcat ttggttcaaa cgagctcatc aagctgcgga cgggggcgag
     2761 cttacctcag gccaatggaa aggcaaggct tctttacaga tgcattcaag attctactgc
     2821 agtcgcgctc aagccggttg agcctccggt gcacgtgcga cacgcatgcg ctggatcaag
     2881 gcgattcctc cgattgcgag aaacccggcc ccaatcggcc agatgtgaga cggtcggtcg
     2941 gccggttcga gcaccgccac gacctcatat ccgacctcaa gcccgatacg cttggcatag
     3001 gagccgaact gcacgttggt gatgctgacg gtgtcaccga gcggcgtcac gccgagcccg
     3061 atcgcccgca agcgctgggg cgctggcgca acgggtccga gcgggatgtt taccgtcttg
     3121 gtgatctcat cgccccggat gttgaaacca gatacgacga agttcaggcg ctcattcgcg
     3181 gg
//
LOCUS       DASUMG010000108         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_897823, whole genome shotgun sequence.
ACCESSION   DASUMG010000108 DASUMG010000000
VERSION     DASUMG010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897823"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VFX46_01755"
     CDS             complement(<1..630)
                     /locus_tag="VFX46_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014327638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX5599340.1"
                     /translation="MDEMTKTSAVPHRPGRYGYDAIRFSGEADALFERHLRFDSVVDP
                     AAAGARDKFEAAAHAVRDILTQRWLQTQQTYDRENPKWVYYLSMEFLIGRSLTNNVTN
                     LMLHSVIGDAVRAAGLDWLGLVEQEPDAGLGNGGLGRLAACFLDSMATMQLPAMGYGL
                     RYEYGMFRQTIENGWQCEHPDHWLRRQDPWEVARPGEAVEIKLGCSIGIS"
     gene            complement(775..>2374)
                     /locus_tag="VFX46_01760"
     CDS             complement(775..>2374)
                     /locus_tag="VFX46_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX5599341.1"
                     /translation="AIDAIRSAARRGGKTGTRPTWPMIVLRTPKGWTGPREVDGHKVE
                     GFWRAHQVPLAEMHEKPAHLKQLEEWLRSYRPEELFDQSGRLVPELRTLAPEGPRRMG
                     ANPHANGGLLRKALSLPDFHAYAVETREPGRIEVSPTQVLGQFLRDVARSNPHNFRVF
                     SPDENASNRLTAIYEATKKTWLAEYLPEDADGGELAPDGRVMEMLSEHTLEGWLEGYV
                     LTGRHGFFSTYEAFVHVIDSMFNQHAKWLEKCTELSWRADIASLNLLITSTVWRQDHN
                     GFTHQDPGFLDVVANKSPAVTRIYLPPDANCLLSVADHCLRSRNYVNVIVADKQPHLS
                     YLDMDEAAKHCAKGAGIWRWASSDQDREPDVVMASCGDIATMEALAATAMLREHFPEL
                     KVRFVNVVDLFRLVPATEHPHGFSDREFDALFTPDKPVIFNFHAYPWLIHRLTYRRTN
                     HDNIHVRGYKEKGNINTPMELAIQNQVDRFTLAIDVIDRVAFLRSRGSHAREALLDKQ
                     LDCRHYAHSEGIDPPEIVNWKWPL"
BASE COUNT          407 a          748 c          768 g          451 t
ORIGIN      
        1 ggagatgccg atggagcacc cgagcttgat ctctacggcc tcgccgggac gggcgacctc
       61 ccatggatcc tggcggcgca accaatggtc cggatgttca cattgccaac cgttctcgat
      121 ggtctgccgg aacatgccgt attcatagcg caggccataa cccatcgccg gaagctgcat
      181 ggtggccatc gaatcgagga aacaggcggc gagccgtcca aggcccccgt tgccgaggcc
      241 ggcgtcaggc tcctgctcca ccagtccgag ccagtcgagc cccgccgcgc gcacggcatc
      301 gccgatgacg gaatggagca tcaggttggt gacgttgttc gtcagcgaac ggccgatcag
      361 gaattccatc gagaggtagt agacccactt cggattttcc cgatcgtagg tctgctgggt
      421 ctggagccag cgttgcgtga gaatgtcacg cacagcatgg gctgcggctt cgaacttgtc
      481 gcgggcgccg gccgcggcgg gatcgaccac gctgtcgaac cgcagatgcc gctcgaacag
      541 cgcatcggcc tccccggaaa agcggatggc gtcatatccg tatcgccccg ggcgatgcgg
      601 tacggcggag gtcttggtca tctcatccat gagtccctct cgtaaagccg cggggatttg
      661 tgatgcaatc gctcgtgcgc aggtgatgcg ccgggccgta ctcaatcgtc atgccactcg
      721 cggcggtggg acggcgcagc aaccaacgcg gtttaacccc aacgcggtca tcaatcagag
      781 cggccatttc caattgacga tctcgggggg atcgatcccc tccgagtggg cgtagtgccg
      841 gcagtcgagt tgcttgtcca acagcgcctc gcgcgcatgc gatccgcgcg acctgaggaa
      901 ggcaacccgg tcgatcacgt cgatcgcgag cgtgaagcgg tcgacctggt tctgtatcgc
      961 caattccatc ggcgtgttga tgttgccctt ctccttgtag ccgcggacgt ggatgttgtc
     1021 gtggttggtc cgacggtagg tcaggcgatg gataagccag ggataggcat ggaagttgaa
     1081 gatgacgggt ttgtcgggcg tgaacagcgc atcgaattca cggtctgaga atccgtgcgg
     1141 atgctcggtg gccggcacca gccggaacag gtcgacgacg ttgacgaacc gcaccttcag
     1201 ctcgggaaaa tgttcccgca gcatcgcggt ggccgccagt gcttccattg tcgcgatgtc
     1261 gccgcaactc gccatcacga cgtcgggctc gcgatcctgg tcgctgctcg cccaccgcca
     1321 gatgcccgcg cccttggcgc agtgcttcgc cgcctcgtcc atgtcgagat aggacaggtg
     1381 tggctgcttg tccgccacga ttacattgac gtagttgcgg ctccgcaggc agtgatccgc
     1441 caccgaaagg aggcaattcg cgtcgggcgg caggtagatg cgggtcaccg ccgggctctt
     1501 gttcgcgacg acatccagga agccgggatc ctgatgggtg aagccgttat ggtcctgccg
     1561 ccagaccgtg gacgtgatca gcaggttgag cgaggcgata tccgcgcgcc aggacagctc
     1621 ggtgcacttc tcgagccatt tggcatgctg gttgaacatc gagtcgatga cgtgcacgaa
     1681 cgcttcatag gtcgagaaaa agccgtgcct gccggtcagc acgtacccct cgagccaacc
     1741 ctcgagcgtg tgctcgctga gcatctccat gacccgacca tccggcgcga gttcgccgcc
     1801 gtcggcatcc tcgggcagat attcggcgag ccaggtcttt ttggtggcct catagatcgc
     1861 ggtcagccgg ttggaggcgt tttcatcagg gctgaagacg cggaaattgt gcggattgct
     1921 gcgggcgaca tcgcgcagga actggccgag cacctgtgtc gggctcactt cgatccggcc
     1981 cggctctcgg gtttcgaccg catacgcgtg gaagtccggc aacgataacg ccttgcgcag
     2041 cagcccgccg ttggcgtgcg ggttcgcgcc catgcggcgc ggaccttccg gagcgagcgt
     2101 ccgaagctcc ggcaccaacc ggccgctttg gtcgaacaac tcctccgggc ggtagctgcg
     2161 cagccattcc tcgagctgct tcaaatgcgc gggtttctcg tgcatctcgg cgagcggcac
     2221 ctggtgcgcg cgccagaacc cttcgacttt gtgtccgtcc acctccctgg gaccggtcca
     2281 gcccttcggg gtgcgcagca cgatcatggg ccatgtcggc cgcgtccccg tcttcccgcc
     2341 ccgccgcgcc gcggaccgga tggcgtcgat cgcg
//
LOCUS       DASUMG010000109         2882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_576212, whole genome shotgun sequence.
ACCESSION   DASUMG010000109 DASUMG010000000
VERSION     DASUMG010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576212"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /gene="ccmB"
                     /locus_tag="VFX46_01765"
     CDS             <1..603
                     /gene="ccmB"
                     /locus_tag="VFX46_01765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5599342.1"
                     /translation="GTALGFYLVVIALMPLGLGPDLQLLSRIAPGVLWIGLLLAALLS
                     LGRMFDVDQEEGVLEILTTGALPLELVVAAKALAHWISSCLPLAILTPILGLLLNLEL
                     AAYPVLVVTVLIGSPAISFLGSVGAALTLRSRRGGLLIALLVLPLYIPTLIFGISAIS
                     AVVTPPGSLEASLLLLSAVTIASLVIGPIAAAAALRAQLG"
     gene            720..1484
                     /locus_tag="VFX46_01770"
     CDS             720..1484
                     /locus_tag="VFX46_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HEX5599343.1"
                     /translation="MQTLTQRLANPTRFMELSERLLPWISGLAALMLAYGLYLAFFVA
                     PPDYQQGETVRIMFVHVPAAWLAMMGYMIIAVSSFGLLVFRHPLADVSAKAAAPIGAA
                     FTLLALITGALWGKPMWGAYWVWDARLTSVLILFFLYIGLIALRSALDEDPTLAGKLT
                     AVLGLVGVVILPVIRFSVDWWNTLHQPASVLRVGGPAIHPSILIPLLVTAAGFTLLFF
                     AMHLKAMRNEILRRRVRSLTMAEVERAVRLEARPAE"
     gene            1489..1677
                     /gene="ccmD"
                     /locus_tag="VFX46_01775"
     CDS             1489..1677
                     /gene="ccmD"
                     /locus_tag="VFX46_01775"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEX5599344.1"
                     /translation="MDLGPHAAFIWASYGVVALVLAVMIAWLIADGRYQQRQLDDFEA
                     RGVRRRSARAADRGNGSA"
     gene            1682..2314
                     /locus_tag="VFX46_01780"
     CDS             1682..2314
                     /locus_tag="VFX46_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HEX5599345.1"
                     /translation="MPDGTVANQQSSGVPARKQRRRLGWLVAPLIIFAALTLMFTFAL
                     KTGDPSRLPSALIGKSAPEFSLKPLPGLVDNGSPVPGFGHDDLGRGKPSVVNFWASWC
                     VPCVQEHPVLVEMVRRTGVDLYGVNYKDQATAARRFLGHYGNPYRAVGVDENGRTAID
                     WGVYGIPETFVVDGEGRIIYKHVGPISPEALETKIIPAIESARKNSSATG"
     gene            complement(2321..>2882)
                     /locus_tag="VFX46_01785"
     CDS             complement(2321..>2882)
                     /locus_tag="VFX46_01785"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HEX5599346.1"
                     /translation="RPDLRFMELGRSCVLRPYRNKRTVELLWHGLWTYVREHKVDVML
                     GCASFEGVDPDAHAMALSFLHYYALAPEEWRVRAHDHLFVPMDRLPKEAVDPRAALKA
                     MPPLIKGYLRLGAVVGEGAVIDHQFGTTDVLIVLPVERIDPRYFDHFGAPDEEGCRVG
                     LDRRADGVEKSSRGARATDELTERPQ"
BASE COUNT          457 a          879 c          900 g          646 t
ORIGIN      
        1 gggacggccc tcggcttcta tcttgtcgtc atcgcgttga tgccgctcgg attagggccc
       61 gacttgcaac ttctgtcgcg aattgcaccg ggcgtgctgt ggatcgggct cctgctggca
      121 gcgctgctct ctctcggccg catgttcgat gtggatcagg aagaaggagt gctggagatc
      181 ctgaccacag gggcgttgcc attggagttg gtcgtcgctg ccaaggcgct ggcgcactgg
      241 atctcctcgt gcctgccgct tgcgatcctg acgccgatct tgggacttct gctgaatctt
      301 gagttggcag cttatccggt gctcgtcgtt acggtgctta tcggctcacc cgcaatcagc
      361 tttctcggca gtgtgggcgc cgcgctgacg ctgaggagcc ggcgtggcgg cctgctgatc
      421 gcgctcctgg tgctgccgct ttatattcca actttgattt tcggtatctc agccatcagt
      481 gccgttgtga ccccaccggg cagcctggag gcctctttac tattgctgtc ggctgtaacc
      541 attgcttccc tggtcatagg cccgatcgct gcggccgctg cactccgggc gcagcttgga
      601 tgacacgtcg cagtcgactt cgcgtagggt gctcgttttg acccagggca atgtccgatt
      661 gctaacccgc gacgtagaag cggtttgcgt ggggccggcc gacggcctag atctgtgcca
      721 tgcagacgct cacccaaagg ttggccaacc cgacgcggtt catggagctg tccgagcggc
      781 ttctgccctg gatttcaggg cttgccgctt tgatgctcgc gtacgggctt tacctcgcct
      841 tcttcgtcgc gccgcctgac tatcagcagg gtgagactgt caggatcatg tttgttcatg
      901 tccccgcggc ttggctggcg atgatgggct acatgatcat cgctgtttcg agcttcggtc
      961 tgcttgtatt ccgtcacccg ctcgcggacg tgtcggcgaa ggctgcagcg cctattgggg
     1021 cggccttcac actgttggcg ctcatcacgg gcgcgttgtg gggcaagccc atgtgggggg
     1081 catattgggt ctgggatgcg cggctcacct cggtgttgat cctgtttttc ctgtatatcg
     1141 gtctaattgc gctgcgttct gcccttgatg aggatccaac gctggcaggc aagctaacag
     1201 cggtgctcgg gcttgtaggc gttgtcatcc taccggtgat tcgtttctcg gttgactggt
     1261 ggaacacgct gcaccagccg gccagtgtgc tcagggtagg cggaccggcg atacatccat
     1321 cgatcctgat cccgttgctg gtgacggctg cagggttcac gctgttgttc ttcgccatgc
     1381 accttaaagc catgcgtaac gagattttgc gacggcgagt caggtccttg acgatggccg
     1441 aagtggaacg agccgttcgt ctcgaagcca gacctgcgga gtagcggtat ggatctcgga
     1501 cctcacgccg cgttcatctg ggcctcatat ggtgtggtcg cgcttgtcct cgcggtcatg
     1561 atcgcatggc tcatcgcgga tggacggtat cagcagcgcc agctcgatga tttcgaagcg
     1621 cgcggggtcc ggcggcgctc ggctcgcgcg gctgaccgtg gcaatggatc ggcataacgc
     1681 catgcctgac ggtaccgtgg cgaatcagca atcatccggc gtgcccgctc gtaaacagcg
     1741 gcgtcgcttg ggttggcttg ttgcgccgct catcatattt gcggcgctca cacttatgtt
     1801 caccttcgct ttgaagaccg gcgacccgtc ccgcctcccg tccgcgctga tcggtaagag
     1861 tgccccggaa ttttcgctca agccgctccc cggacttgtg gacaatggct cgccggtgcc
     1921 cggctttggc cacgatgacc tcggccgcgg gaagccatca gtcgtcaatt tctgggcctc
     1981 ctggtgcgtg ccgtgcgtgc aagagcatcc cgttcttgtc gagatggtgc gccgaaccgg
     2041 cgtcgacctg tacggcgtca actacaagga ccaggcaacg gctgcacgcc gcttccttgg
     2101 ccactacggc aacccttacc gggccgttgg tgtcgatgag aacggccgca ctgcaatcga
     2161 ttggggtgtc tacgggattc cggagacctt cgtcgtcgat ggcgagggac ggatcatcta
     2221 caagcacgtg gggccaattt cgccagaggc gctcgagacg aaaataatcc cggcgataga
     2281 atctgcgcgg aagaactcgt cagctacggg gtgaagccgt ttactgcggt ctctccgtga
     2341 gttcgtccgt ggctcgtgct ccccgcgaag atttttcgac gccgtcggcc ctacgatcga
     2401 gcccaacgcg gcaaccctct tcatcgggtg cgccgaagtg gtcgaaatag cgcggatcga
     2461 tccgctccac cggcagcacg atcagcacgt ccgtcgttcc gaactggtga tctatgaccg
     2521 cgccttcgcc gaccacagcg ccaagccgga ggtacccctt gatgagcggc ggcatggcct
     2581 tgagagccgc gcgcgggtcg actgcctcct tcggcaaacg gtccattgga acgaacaggt
     2641 gatcgtgggc tcgcacccgc cactcctccg gtgccaacgc atagtagtgg aggaacgaca
     2701 gcgccatggc atgtgcatcg gggtcgacac cctcgaagct ggcgcacccc agcatgacgt
     2761 ccactttgtg ctcgcggacg taggtccaga gcccgtgcca gagcagctcc accgtgcgct
     2821 tgttgcggta aggcctcagg acgcagctgc gccccagctc catgaaccga aggtccggcc
     2881 tt
//
LOCUS       DASUMG010000110         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_174964, whole genome shotgun sequence.
ACCESSION   DASUMG010000110 DASUMG010000000
VERSION     DASUMG010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174964"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1137
                     /locus_tag="VFX46_01790"
     CDS             22..1137
                     /locus_tag="VFX46_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5599347.1"
                     /translation="MPQEALSHCDAVVIGEVELVIDKLLDDLEQDEMRGTYKSPTLHP
                     MTGLSMPRYDLLKKHRYVNKTFVQTSRGCHQGCTFCAEPLMNGLRFRYRPVDEVIHEM
                     ENCGSRIISINDADFFGTPERPKEVMRALKGRNIQWQAGVTSKLAQDDRMLELAAESG
                     CTLLSIGFESISRATLTSVHKHVNRPETFAALVEKVHSYGIMVFGLFMYGFDGDDLSV
                     FDDTVKFNRDAGYDACAYSTLTPYPGTLTWYEMRKANRIVSFDWTMYDQGHVVYRPAQ
                     MSPDELRIGVGRAYENFYSASSMAQRFPLRGKRHRAQWVIYNLFMRRGSQTENIESIA
                     APTAEPGFAPMPPILPVKREWRTAVLEAAGGPGPHMS"
     gene            1441..>2137
                     /locus_tag="VFX46_01795"
     CDS             1441..>2137
                     /locus_tag="VFX46_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5599348.1"
                     /translation="MPPRGQTWTTLPALQQSHMNAFHEYDKGITGIGAGFAIGQRAIL
                     VQTEGGNILWDCVATLDPATVSLIKGMGGLKAIAISHPHFYTTMNEWAQAFNCPIHLN
                     AADQEWIMRKGPAIKLWQGDTFKLWDGVTLVRCGGHFPGGTVGHWAGGAGGRGIVCAG
                     DILTITQDRKWFSFMRSYPNFLPLSKREVEHIGKAMAPFKFEVVYGHYFDRVIANDAK
                     GLLEKSVARYVAAI"
BASE COUNT          435 a          636 c          661 g          405 t
ORIGIN      
        1 ggcggggtgc atccgagctt catgccgcag gaggcgctga gccattgcga cgccgtcgtc
       61 atcggcgaag tcgagctggt gatcgacaaa ctgctcgacg atctggagca ggacgagatg
      121 cgcggcacat acaagtcacc cacgctgcat ccgatgacag gtctgtcgat gccgcgctac
      181 gaccttctga agaaacatcg ctacgtcaat aaaacctttg tccagacttc gcgtggctgc
      241 caccagggct gcacgttctg cgccgagccg ctgatgaacg gcctcagatt tcgctatcgg
      301 ccagtcgatg aagtgatcca tgaaatggag aattgcggct cgcgcatcat ttccataaac
      361 gacgccgatt ttttcggaac tccggagcgt ccgaaggaag tgatgcgcgc gctcaagggg
      421 cgcaacatcc agtggcaagc cggcgtgacg tcaaagctgg ctcaggacga tcgcatgctc
      481 gagctggcgg cggagagcgg ctgcacgctg ctgagcatcg gttttgaatc gatatcgcgc
      541 gccacgctca ctagcgtgca caagcacgtc aaccggcccg agaccttcgc ggctttggtc
      601 gagaaggttc actcctacgg aatcatggtg ttcggcctgt tcatgtacgg gttcgatggc
      661 gacgaccttt cggtgttcga cgacaccgtc aagttcaacc gtgatgccgg ctacgatgcc
      721 tgcgcctatt caaccttgac gccttatccc ggcacgctga cctggtatga gatgaggaag
      781 gccaatcgta tcgtgtcctt tgactggacg atgtacgacc agggtcacgt ggtctatcgg
      841 ccggcccaga tgtcaccgga cgagcttcgc attggtgtcg gccgcgccta cgagaatttc
      901 tattcggcct catcgatggc ccagagattt ccgctgcgcg gcaagcggca tcgcgcccaa
      961 tgggtgatct acaatctttt catgcgccgg gggtcgcaga ccgaaaacat cgagtcgatc
     1021 gcggcgccca ccgcggagcc gggttttgct ccaatgcccc cgattctgcc ggtcaaacgc
     1081 gaatggcgta cggcggtgct tgaggccgcc ggagggcccg ggccgcacat gagctgatcg
     1141 cgtcctaccg gtcattccgg gacggcgcgc cagcgccgga caacacactc cgctcattcc
     1201 cgcggaagcg ggaattccag agttcattgc cctggatccc cgtgttcgcg gggatgagcg
     1261 gagaggggtc ggcaaccctc gggcgctttg caaaaagggc gagacccaac acaatgggcc
     1321 acgaacgaaa aagccgagga actcatgcct ctttacatct gtaccgcctg cggcacgcaa
     1381 tatcctgaaa gcgcgcagcc gcccgcgcaa tgcacaatct gcgaggagga gcggcaatac
     1441 gtgccgccgc gcggccagac ctggacgacg ctcccggcgc tgcagcagag tcacatgaac
     1501 gcgttccacg aatacgacaa aggcattacg ggaatcggtg ccggctttgc catcgggcag
     1561 cgcgcgatcc tggtgcagac ggaagggggc aatattctct gggactgcgt ggcgacgctc
     1621 gatcctgcga cggtcagcct catcaagggg atgggcggcc tgaaagcgat cgcgatctcg
     1681 catccgcatt tctacacgac gatgaatgaa tgggcgcagg ccttcaactg cccgatccat
     1741 ctcaacgccg ccgatcagga atggatcatg cggaaggggc cggcgatcaa gttgtggcag
     1801 ggcgacacct tcaagctttg ggatggcgtc actttggtcc gctgcggcgg gcactttccg
     1861 ggcggcacgg tcggtcactg ggcgggcggc gccggcgggc gagggatcgt ctgcgcggga
     1921 gacattctga ccattaccca ggaccggaaa tggttttcct tcatgcgcag ctatccaaat
     1981 tttcttccac tgtcgaagcg cgaggttgag cacatcggca aggccatggc gccgttcaag
     2041 ttcgaggtcg tgtacggaca ctatttcgac cgtgttatcg caaacgatgc aaaaggcctg
     2101 ctggaaaaat ccgtcgcccg ctatgttgcg gccatcg
//
LOCUS       DASUMG010000111         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_898034, whole genome shotgun sequence.
ACCESSION   DASUMG010000111 DASUMG010000000
VERSION     DASUMG010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898034"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..882)
                     /locus_tag="VFX46_01800"
     CDS             complement(175..882)
                     /locus_tag="VFX46_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ family molecular chaperone"
                     /protein_id="HEX5599349.1"
                     /translation="MPWRAITRVIGLGEGGAVRAVLDGFYANLGLDKLWRSRSQRNSV
                     AFTIAVISLCAKLSKADGVSTCLEAQAFEEIYHVPPEERANVERLYNIAKQDVAGYDA
                     YADKISRLLADEPDLKRNVLEALFHIAAADGIFHGAEDAYLADVARRFGFSDAEYRAI
                     RSLFVREPNDPFTVLGVQPDIPNDELKAHYKRLVREHHPDTLMARGVPKEFIDVANRK
                     LAALNAAYDEIAKERAL"
     gene            complement(1314..>2400)
                     /locus_tag="VFX46_01805"
     CDS             complement(1314..>2400)
                     /locus_tag="VFX46_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5599350.1"
                     /translation="YRTRLAEDMFEGLAPWLSRLPGRLLHTSVVGCAIFAAVSGSSAA
                     TTATVGRITLPELQKRGYDERMTIGTLAGSGTLGLLIPPSIILIVYATATDQSIARLF
                     IAGVVPGILLAGLFMGYVMVWALLFRHRMPPPEPATTFVQKVKASRRLLPVVLLILGI
                     IGSIYGGLASPTEAAVVGVVLSLALSWSTGSLTRENATDALLSATRTSCMICFILAGA
                     AFLTTAMGFTGIPRELAEWVGSFNLSKYQLLFALTLFFIVLGCFLDGISIVVLTTSII
                     IPMVERTGFDLIWFGIYIVIVTEMAQITPPVGFNLYVLQSMTGRNLFTIGLYALPLFM
                     LMVVAVALLAAFPELALWLPSTMLDAR"
BASE COUNT          525 a          742 c          721 g          412 t
ORIGIN      
        1 catagtgggc cgacaccttg ctttccggcc gcgcgagcca gtcgattgcg ccacggcatg
       61 tcggcacgcc ggtatagtgg agcagaagaa tggtcggctg gcggtcgtcg cggcgcggct
      121 cgaaattcgg tgacggacag acgtcgctga ccagtgagct gtcaggctcg aacgtcacag
      181 cgcgcgctcc tttgcaatct catcgtatgc ggcattgagg gccgcgagct tgcggttggc
      241 cacgtcgatg aactccttcg gcacgccacg cgccatcagc gtatcgggat ggtgctctcg
      301 cacaagccgc ttgtagtggg ctttcaattc gtcgttcgga atatccggct ggacgccgag
      361 aaccgtaaaa ggatcgtttg gctcacgaac gaaaaggctg cgaatggcgc gatactccgc
      421 gtccgagaag ccaaagcgcc gcgccacatc tgctagatag gcatcctctg caccatggaa
      481 gatgccgtcc gccgctgcga tgtggaacag ggcctccaga acgttccgct tcaggtccgg
      541 ctcatcggcg agcagccgtg agatcttgtc tgcataagca tcataacctg caacgtcctg
      601 cttcgcgata ttgtagaggc gctccacgtt ggcgcgctcc tccggcggca cgtggtagat
      661 ctcctcgaag gcctgcgcct ccaagcaggt cgagacgcca tccgccttgg acagctttgc
      721 acaaagcgag atcaccgcga tggtgaacgc gacgctgttg cgctgtgaac gggagcgcca
      781 gagcttatcc aggcccaggt ttgcgtaaaa gccatccagc accgcgcgaa cggcgccacc
      841 ctccccaagc ccgatgacac gtgtgatggc tcgccaaggc atacacctgc cttcttaccc
      901 tcgatcacgg cgacaatgaa gccacttggc gacgcatcgg gccgtgggct gcgtgccaca
      961 gatcggccaa aatccgccac ggtttaacca ttattcgagt tgcttcggcc attttgatgt
     1021 cgacttcatg gcatggcgtg ctagcttttt ttgccacaaa gctcaaggag ccgtcactaa
     1081 cgaacaaggg cgggggaacc atgagaacgc tttgctacgc cattgctgct gcggctatga
     1141 ttgccgtggc tgccggaacg ggcaagtcgg aggatccaac atcggatatc aaggctcagg
     1201 tccctgttca aacggcatct gtttccatca gccgttgatt gcgacctgag gcgcggcaag
     1261 tcgttcagcc cgtgcggccg cgcccaacat gatgacgccg aaaaaggcac cattcagcgc
     1321 gcatccagca tcgtcgaagg cagccagagc gccaattccg gaaaggccgc taacagggcc
     1381 accgccacca ccatcagcat aaagagcggc agggcataga ggccgatcgt gaacaggttc
     1441 cgcccggtca tcgactgcag aacgtagagg ttgaacccga ccggcggtgt gatctgtgcc
     1501 atctccgtga cgatcacgat gtagatgccg aaccagatca ggtcgaagcc ggtgcgctcg
     1561 accatcggga tgatgatcga ggtggtcagc accacgatcg aaatcccatc caagaagcac
     1621 ccgaggacga taaaaaacag cgtcagcgcg aagagcagct gatatttgct caggttgaag
     1681 ctccccaccc attccgcgag ctcacgcgga ataccagtga accccatggc cgtggtgagg
     1741 aaggccgcgc cagcgaggat gaagcagatc atgcaagatg tccgcgtggc cgacagcagc
     1801 gcatccgtgg cattctccct cgtcagactg cccgtggacc aggagagtgc gagcgacagc
     1861 acaaccccta cgactgcggc ctccgtcggt gaggcgagcc cgccatagat cgagccgatg
     1921 atgcccagga tcagcagaac caccgggagc agacggcgcg acgccttcac tttctgaacg
     1981 aacgttgtag ctggctccgg aggcggcatt cgatgccgga acagcaaggc ccagaccatc
     2041 acgtagccca tgaacagccc ggccagaaga attcctggaa ccacgccggc gatgaacagc
     2101 cgcgcgatcg actgatccgt ggcggtcgca tagacgatca ggatgatcga tggcggaatg
     2161 agcagtccga gcgtgccgga gccggcgagc gtgccgatcg tcatacgctc gtcgtagccc
     2221 cgcttctgca gctctggcag ggtgatacgc cccacggtgg ccgtcgtcgc agccgacgag
     2281 cctgacaccg cagcaaagat tgcgcagcca acaacgctcg tgtgcagcag acgccccgga
     2341 agacgcgata accaaggtgc caggccctcg aacatgtcct cggccagacg cgtgcggtag
//
LOCUS       DASUMG010000112         4448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_576644, whole genome shotgun sequence.
ACCESSION   DASUMG010000112 DASUMG010000000
VERSION     DASUMG010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576644"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VFX46_01810"
     CDS             <1..881
                     /locus_tag="VFX46_01810"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5599351.1"
                     /translation="DLAVQELTAAISGGKISQSTLARALYYRGLAYRKQNKPAQAIAD
                     FTSALWIKNGLDPEQRADALMNRAAAYREAGLTDQAEADEKRVAATREAAGARTASGE
                     SRPAPSSTTTASQSSSGGGFGSFFSALFGGSSQSSQSSSAQRSSAASYSSSGDQQAAE
                     SRRSPTVSAWSSQVHPAAGGWSDSTVVRKASTSNATRSAPTTTASTGRSTGSGRFIQV
                     AAVRNRQEAQTVAARLKQLGMTEGQQDAAIEETIMGNMGKLYSVRLGPFASADEVKSV
                     CPRLRQAGLDCLVLSR"
     gene            1039..1824
                     /locus_tag="VFX46_01815"
     CDS             1039..1824
                     /locus_tag="VFX46_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX5599352.1"
                     /translation="MQKSEAEQQPQARSGADTKANPLPWLGTAQSHSDLRIGARLRNY
                     FLTGLVVVGPVTITLYIGWWFINVVDAWVKPFVPSVYNPETYLPFPMPGIGLVFGIVG
                     LTLIGALAANLIGRTIISSGEMFLQRMPIVRNVYQALKQIFVSVVSAAGPGTQFNKVG
                     LMEFPSKGIWSLVYITGEVRGEIKEVRPGGEDDLLTVFMPTGIVPPTGFVCVVPRRDV
                     IPLNMTVEDVAKMVISAGMVMPEYQKQLKNLADAARGENVEKT"
     gene            complement(1846..3960)
                     /gene="recG"
                     /locus_tag="VFX46_01820"
     CDS             complement(1846..3960)
                     /gene="recG"
                     /locus_tag="VFX46_01820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX5599353.1"
                     /translation="MRPSILTPLFASVQSLAGVGPRVAVLLKKALRLPTGVTEPRVID
                     LLWHAPTGVIDRRAEPTIAGAVPGTIATFAVRVLRHKAPPPGNPKVPHKVTCEDDTGR
                     IDLVFFHAERRFIERQLPVGSIRYISGRVERYGDTLQMAHPDYIVPPEARADLPMLEP
                     VHPLTAGLSGKILLKIMRQAVERVPDLPEWQEPRWLALRGWPSFREAVTRLHVPRDSD
                     DVSPVAPHWQRLAYDELLAGQVALALVRQRLKSQPGRAIVGDGRIRRQLKAALPFALT
                     PSQVKALSEIEADMAAPRRMLRLLQGDVGSGKTAVALFAMATAVEAGTQAALMAPTEV
                     LARQHVETIAPLAEKAGLRVGLLTGREKGKARAEILARLARGDIDILIGTHALFQPDV
                     VFKDLAFAVIDEQHRFGVHQRLALQTKGGQGVADVLVMTATPIPRTLLMTHYGDLDVS
                     RLTEKPAGRKPVTTTLVSIDRLEEVIDGLRRALADGAQVYWVCPLIEGNGASELAAAE
                     ERYAHLNQVLGGKVGLLHGAMPSRVKDATMAAFAEGKLRVLVSTTVIEVGVNVPNATI
                     MVIEHAERFGLAQLHQLRGRVGRGERQSYCVLLYKPPLGEIAEERLKIMRETEDGFLI
                     AEKDLELRGGGEVLGARQSGLPEYRIANVPNFEELLAAARDDARLLLHEDPSLTSPRG
                     AAIRTLLYLFECDDAVRLFRAA"
     gene            4143..4409
                     /locus_tag="VFX46_01825"
     CDS             4143..4409
                     /locus_tag="VFX46_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEX5599354.1"
                     /translation="MRGDELDVRRRRAAYRATHRGTKEMDWVIGRYAVSVLPNLEGEA
                     LDRLERFLNLPDPDLQQWFYSPHLAAGTEFSELVAEMRRFHGLE"
BASE COUNT          803 a         1384 c         1472 g          789 t
ORIGIN      
        1 cggatctggc ggtgcaagag ctgaccgcgg ccatttcagg aggcaagatt tctcagtcga
       61 ccttggcgcg cgcgctgtat taccgcggcc tcgcctaccg caagcagaac aagccggcgc
      121 aggcgattgc cgatttcaca agcgcgctgt ggatcaagaa cgggcttgat cccgagcaaa
      181 gggcggatgc cctaatgaat cgcgctgccg cttaccgtga ggcaggcctg accgatcaag
      241 ccgaggctga cgagaagcga gtggctgcga cgcgagaggc agctggagcc aggacggcct
      301 cgggcgagag tcgcccagct ccatcgtcga ccacgacagc ttcccaatct tcttcgggcg
      361 gcgggtttgg cagcttcttc agcgcgctgt tcggcggcag ttcccaatct tcacagagct
      421 catccgctca gcgctcatcg gcagcatcct acagctcatc gggtgaccaa caagccgctg
      481 agtctcgccg ctcaccgacc gtttccgcgt ggtccagtca ggtccatcct gcggcgggcg
      541 gttggtcgga ttccacagtg gtgcgcaagg cgagcacgtc caacgccaca cgcagcgcgc
      601 cgacgaccac cgcttcgacg ggaaggtcca cgggttcagg ccggttcatc caggtggcgg
      661 ccgtccgtaa ccggcaggag gcgcagactg tagcggcgcg cctcaagcaa ctcgggatga
      721 ccgaggggca gcaagacgcg gccattgagg agaccatcat gggcaacatg ggcaagctct
      781 acagcgttcg cctggggccc ttcgcaagtg ctgacgaggt gaaaagcgtt tgcccgcggc
      841 tccgtcaggc tggtctcgac tgtctggtcc tcagccgtta gggtcgctgc tccccctcgc
      901 gatcgtttgc acgcccgaca cttatcgcct attgcaccca gagcctaatc cggcttcctt
      961 gatgcgggag accagcatac gggaggagct cggctcgcgt gctcgaattg tcgcgcgcgg
     1021 cgggatggat atcgagacat gcagaagagt gaagcggaac agcagccgca ggcgagatcg
     1081 ggcgcagaca cgaaggcgaa cccgttgccg tggcttggta ccgcccaatc tcattcagac
     1141 ctgcgcatcg gcgctcggtt gcgcaactat ttcctgactg gactcgtcgt cgtcggaccc
     1201 gtcaccatca cgctctatat cggctggtgg ttcatcaacg tggtggacgc ctgggtgaag
     1261 ccgttcgtgc ccagcgtcta caatcccgaa acctatcttc cgtttcccat gccggggatc
     1321 ggactggttt ttgggatagt cggcctgacg ctgatcggcg cactggccgc aaacctcatt
     1381 ggccggacga tcatcagctc cggcgagatg ttccttcagc gcatgccaat cgtgcgcaac
     1441 gtctatcagg ccttgaagca gatcttcgtc tccgtggtga gcgccgcagg gcctgggacc
     1501 caattcaata aagttggctt gatggagttt ccgtccaaag gaatttggtc gctcgtctac
     1561 atcacggggg aggtgagagg cgagataaag gaggtccggc ctggaggcga ggacgacctg
     1621 ctcacggtct tcatgccgac ggggatcgtg cctcccacgg gcttcgtctg tgtcgttccc
     1681 cgccgggatg tcatcccgct gaacatgacg gttgaggatg ttgcgaagat ggtcatctcg
     1741 gccgggatgg tgatgccgga gtatcagaag cagctgaaaa acctggccga tgcggcgcga
     1801 ggcgaaaacg ttgagaagac gtgacttggc aggctgcttc tgggctcacg ctgccctgaa
     1861 cagccgcaca gcatcatcgc actcgaagag atagagcagc gtgcgaatgg cagcgccgcg
     1921 ggggctggtg agcgatggat cttcgtgcag cagcaagcgg gcgtcgtcac gggcggcggc
     1981 cagaagctcc tcgaagttcg gcacattggc gatgcgatac tcgggaaggc cgctttggcg
     2041 tgcaccaagc acctcgccgc caccacgcag ctccaaatcc ttctcggcta tgaggaagcc
     2101 gtcctccgtc tcgcgcatga tctttaggcg ctcctcggcg atctccccga gaggcggctt
     2161 gtaaagcagc acgcaatagg attgccgctc gccgcgtcct acgcgcccgc gcagctgatg
     2221 aagctgggcg aggccaaatc tctccgcgtg ctcgatgacc atgatggtcg cgttgggaac
     2281 gttgacgcca acctcgatga cagttgtcga gacgagcacc ctcagcttgc cttcggcgaa
     2341 ggcggccatg gtcgcatcct tcacgcggct cggcatggcg ccatgcagca gaccaacctt
     2401 cccgccgagc acttggttga ggtgcgcgta gcgctcctca gcggccgcaa gctcggaggc
     2461 gccattgccc tcgatgagcg ggcacaccca atagacctgc gcgccatcgg ccaaggcgcg
     2521 ccgcagaccg tcgatgacct cctccaaacg atcgattgag acgagggtag tggtcacggg
     2581 cttccggccg gccggcttct cagtgaggcg agatacgtcg agatcgccgt aatgcgtcat
     2641 caggagcgtg cgggggatgg gggtggccgt catcaccagc acgtcggcca caccctgtcc
     2701 gcctttcgtc tgcaacgcca gccgctgatg gacgccgaag cggtgctgct cgtcgatgac
     2761 ggcaaaagcc aaatccttga agacgacgtc cggctggaag agagcgtgag tgccgatgag
     2821 gatgtcgatg tcgccgcggg caagacgggc aaggatttcg gctcgcgcct tgcccttttc
     2881 gcggcccgtc agcaacccga cgcggagccc ggccttctcg gcgaggggcg cgatggtctc
     2941 gacgtgctgc ctcgccaata cttccgtggg cgccataagg gcggcctgtg tgcccgcctc
     3001 gacggcggta gccatggcaa aaagggccac ggctgtcttg cctgagccga catcaccttg
     3061 cagcagccgt agcatccggc gcggcgccgc catatccgcc tcgatttccg aaagggcttt
     3121 gacctgggaa ggtgttagcg cgaacggcag ggccgccttg agctgccggc gaatgcggcc
     3181 gtcaccaacg atggcccggc cgggctggga tttgaggcgt tgccggacca gggccagtgc
     3241 cacttgtccc gcgagcagct cgtcgtaagc gaggcgctgc cagtggggtg ccaccggcga
     3301 gacgtcatcg gaatcgcggg gcacgtggag gcgcgtcacg gcttctcgga acgatggcca
     3361 gccacgcaag gcgagccagc gcggctcctg ccattcggga agatccggca cgcgttcgac
     3421 ggcctgccgc atgatcttca gcaggatctt ccccgagagg cccgccgtga gcgggtgaac
     3481 cggctccagc atcggcaggt cggcgcgggc ctcgggcggc acgatgtaat ccgggtgcgc
     3541 catctgcagc gtgtcgccgt accgctcgac ccggccggag atgtagcgga tgctgccgac
     3601 ggggagctgc cgctcgatga atctccgctc cgcgtggaag aagacgagat cgatgcgtcc
     3661 ggtatcgtcc tcgcaggtga ccttgtgcgg caccttggga ttgccagggg gcggtgcctt
     3721 gtgcctcagc acacggaccg cgaaggtcgc gattgtcccg ggcactgcgc cagcgatcgt
     3781 cggctcggct ctccggtcga tgactccagt cggcgcgtgc cacaacaggt cgatgacacg
     3841 tggctccgtg acaccggttg gcaggcgcag ggccttcttg agcagcacgg cgacccgcgg
     3901 gcccacacct gccagcgatt ggacggaggc gaagagcggt gtcagtatgc tcggacgcat
     3961 cgcggagacg ccggtcccat atcggagcca ttcctcaaac gcagaggagc cggtgcgtcg
     4021 cggctcactc tttgcgcatg gccttcctga ggctatatga cgcccatccc ccttgcacgt
     4081 tggcgaggca gggtcggggc gtcaaggcgc gcggctgatt atccagaggt ggtgaggacg
     4141 tgatgcgagg cgatgaactc gacgtgcgcc ggcggcgtgc cgcctaccga gccacgcacc
     4201 gcggcaccaa agagatggat tgggtgattg gccgctatgc cgtctcagtc ctgcccaatc
     4261 tggaagggga ggcgctcgat cggctggagc gctttctgaa cctgccggac cccgatctgc
     4321 agcaatggtt ctactctccg catctggccg ctggaaccga attttctgag ctggtagcgg
     4381 agatgcgccg ctttcatggt ctggagtgac ggcggctagg acgcaagcac gagagcggcg
     4441 gcccgaaa
//
LOCUS       DASUMG010000113         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1139246, whole genome shotgun sequence.
ACCESSION   DASUMG010000113 DASUMG010000000
VERSION     DASUMG010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139246"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFX46_01830"
     CDS             <1..868
                     /locus_tag="VFX46_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5599355.1"
                     /translation="IRLLRRFNSEVFSRFPQATTAAEESTAWPMVSRPVDWGGLGFGY
                     KWNMGWMHDTLDYISKDPIYRRHHHGQILFGLHYAFFENFILPLSHDEVVHGKRSILG
                     RMPGDEWQRFANLRAYYGFMFGHPGKKLLFMGNEFAQENEWRHDHSLDWHLLDKPAHA
                     GIQALVRDLNHLYRSVPALHALDCEDKGFEWLIMHDADRSVFAWLRKGRDTHERCLVV
                     VNFTPQVYRDYRIQVPFSGIWREVLNTDSAAYGGSDVGNGGAVRTWDEGAIPEVSLVV
                     PPLAAIFLVPER"
     gene            870..2945
                     /gene="glgX"
                     /locus_tag="VFX46_01835"
     CDS             870..2945
                     /gene="glgX"
                     /locus_tag="VFX46_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX5599356.1"
                     /translation="MRLSAGEPHQLGATWDGRGANFALFSANAEKVELCLFDSSGRRE
                     IERIALPERTEDVWHGYLNDVAPGQLYGYRVHGRYEPERGFRFNPHKLLIDPYAKQLT
                     GRIVWSDAHFGYRAGSARADLSFDRRDNARGMPKGVVIDQAFTWGEERRPSVPWEDTV
                     IYEAHVKGLTQQRGDVPPAWRGTFRGLAAPGVIGHLQRLGVTALELLPIHAFVDDRHL
                     VERGLNNYWGYNTLGFFAPAQRYALERPLDEFRSTVSRLHDAGIEVILDVVYNHTAEG
                     NHLGPTLNFRGIDNTSYYWLLPDQPRYYDDFTGCGNALNLTHPRVLQMVMDSLRYWVD
                     ICHIDGFRFDLASTLGRGPNGFDRGAAFFAALRQDPALAAAKLIAEPWDIGPGGYQVG
                     TFPPGWSEWNDHFRRTLRRYWMGEGNIIGELGRRMTGSADLFDHDRRSPRSSVNHVTV
                     HDGFTLADLVSYQRKHNLANGEHNRDGSDDNHSTNCGVEGATDKPEVLKLRRQLRRNL
                     LASLMLAQGVPLLLAGDEVGNSQQGNNNAYCQDNPVGWVDWSALGGDDEDDLSSFVAQ
                     LTELRRRFPQLRARRWVQGRRADGSFGVLWLTPEATEMAEQDWHFPQGRFLSYVLGPL
                     QQGKPPLYLVFNAATEAIEFTLPILQEYGRWTALLATAPVPRVEEEFASGSKLEAPPR
                     SILAFAGAT"
BASE COUNT          555 a          905 c          902 g          613 t
ORIGIN      
        1 gatcaggctc cttcgtcggt tcaattcgga ggtgttctca cggtttcccc aagcgaccac
       61 agcagcagag gaatcgacgg cttggccgat ggtttcgagg ccagtcgatt ggggcggtct
      121 gggcttcggc tataagtgga acatggggtg gatgcacgac accctcgact acatcagcaa
      181 agacccgatc taccgccgcc accatcacgg acaaatcctg tttggcttgc actacgcatt
      241 cttcgagaat ttcatcctgc cgctgtcaca cgacgaggtc gtgcatggga agcgttccat
      301 tctcggccgc atgccgggag acgaatggca gcgcttcgcc aatttgcgcg cctactacgg
      361 attcatgttt ggccacccgg gcaagaagct gctgttcatg ggcaatgaat tcgcccagga
      421 aaacgaatgg cgtcacgacc attcgcttga ttggcatctc ctcgacaagc cggcgcatgc
      481 ggggatccag gcattggtcc gggatctcaa tcacctttac cgatccgtgc cggccctgca
      541 cgcccttgat tgcgaggaca agggattcga atggctgatc atgcacgacg ccgatcgcag
      601 cgtctttgcg tggctacgga aaggacgcga tacgcatgag cgttgcctgg ttgtcgtcaa
      661 cttcacgcct caggtctacc gcgactatcg cattcaggtt cctttctccg ggatctggcg
      721 cgaggtcctg aacaccgact cggcggccta tggtggcagc gatgtcggga acggcggcgc
      781 agtcaggaca tgggatgagg gcgcaattcc cgaggtcagc cttgttgttc cgccgcttgc
      841 agcaattttt ctcgtcccgg agcgctgatt tgcggctttc ggctggcgaa ccgcaccagc
      901 tgggtgcgac atgggacggc cgaggcgcga atttcgcgct gttttcagcg aatgccgaga
      961 aggtcgaact gtgtctcttc gacagtagcg gccgccgcga aatcgaacgt atcgcgttac
     1021 ctgagcgcac tgaggatgtc tggcacggct atctgaacga cgtcgcaccc ggtcagctct
     1081 atggctatcg ggttcacggc cgctatgaac ccgagcgcgg cttccgcttc aaccctcaca
     1141 agctgctcat cgatccttat gccaagcaac tcaccgggcg catcgtctgg agcgatgccc
     1201 atttcggcta tcgcgccggc agcgcgcgcg ccgatctgtc tttcgatcga cgggataacg
     1261 cgcgcggaat gccgaagggc gtcgtcatcg accaagcttt cacctggggg gaggagcgca
     1321 ggccatcggt tccctgggaa gacacagtca tctacgaagc tcacgtcaag ggactgacac
     1381 agcaacgcgg cgacgtcccg ccggcttgga ggggtacctt ccgcggactc gcagcgccgg
     1441 gcgtcatcgg ccatcttcaa cggcttgggg tgaccgcgct cgagctgctc ccgatccatg
     1501 ccttcgtcga cgatcgacac ttggtcgagc gcgggctgaa caactactgg ggatacaaca
     1561 cgctcgggtt ttttgcacca gcgcaacgct atgcgctgga gcggccgctc gacgaatttc
     1621 gttcaaccgt ctcgcgcctg cacgatgccg gtatcgaggt catccttgac gtcgtttaca
     1681 accataccgc cgaaggcaat cacctcggcc ctacactgaa ctttcgcggc atcgacaaca
     1741 cgtcatatta ttggctgtta ccggaccagc cgcgctatta cgatgacttc accggctgcg
     1801 gcaatgcgct taatctcacc catccccgtg tgctgcagat ggtgatggat tcgctgcgct
     1861 attgggtcga tatctgccac atcgatggat ttcggttcga tcttgccagc acgcttgggc
     1921 ggggccccaa cggatttgat cgcggagcgg cgttcttcgc cgccctccgg caagatccgg
     1981 cgttggccgc agccaagttg atcgccgagc cttgggacat tgggcccggc ggatatcagg
     2041 tcggcacgtt tccaccgggc tggtcggagt ggaacgacca ttttcgtcgc acgcttcgcc
     2101 gctattggat gggcgaaggc aatatcatcg gtgagctcgg tcgccgcatg accggttctg
     2161 ccgatctatt cgatcacgat cgtcgcagtc cacgatcgag cgttaatcac gtcactgtcc
     2221 acgacggatt cacgctcgcc gacttggtca gctatcagcg taagcataat ctggcgaacg
     2281 gcgagcataa ccgcgacggc tcggacgata accacagcac gaactgtggt gtcgagggag
     2341 ctacggacaa gcccgaggtc cttaagctcc ggcgtcagct gcggcgcaat ctgctcgcaa
     2401 gcctgatgtt ggcgcagggc gttccattgc tgctggccgg agacgaggtt gggaacagcc
     2461 agcagggcaa taacaatgcc tactgtcagg acaacccggt cggatgggtg gattggtcgg
     2521 cgctgggtgg cgatgacgag gacgaccttt cctcattcgt ggcgcagctc accgaactgc
     2581 gtcggcgttt tccccaattg cgcgcgaggc gatgggtcca aggacgtcgt gccgatggct
     2641 cctttggcgt cttgtggttg acgcccgagg ccaccgagat ggcggagcag gactggcatt
     2701 ttccccaagg acgcttcctg tcctatgttc taggtcccct gcagcaagga aagccacccc
     2761 tctatctcgt tttcaacgcg gcgacggagg cgatcgagtt cacgctccct atcctccaag
     2821 agtacggccg ttggaccgcg ctgctggcaa ccgcgccggt gccccgcgtg gaggaggaat
     2881 tcgcgtcggg ctcgaagctg gaggcaccgc cgcgatcgat ccttgcgttt gcgggcgcga
     2941 catgacggcg tgcgcacgcc cggacgcagc cctca
//
LOCUS       DASUMG010000114         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1058952, whole genome shotgun sequence.
ACCESSION   DASUMG010000114 DASUMG010000000
VERSION     DASUMG010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058952"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..1092
                     /locus_tag="VFX46_01840"
     CDS             505..1092
                     /locus_tag="VFX46_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599357.1"
                     /translation="MYLYRLMMAESAGKPHAKNPRSSALGPYQFINSTFIEVVDKHFA
                     DEVQGKSRSEILALRTDMEFARRAAIAFNNDNAKYLEERGVKPTFAHLRLSHLLGPAA
                     AVKVLRAKPNTRLTALLPSSVIRANPFMRRMTVAMLVRWSEREVGVGVWSTPSEIAKS
                     DESESSATEAKLEEAVDAPSEKAELEVAEDNNRAL"
     gene            complement(1182..2279)
                     /gene="ychF"
                     /locus_tag="VFX46_01845"
     CDS             complement(1182..2279)
                     /gene="ychF"
                     /locus_tag="VFX46_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5599358.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAEAANYPFCTIEPNVGE
                     VGVPDPRLEKLASIAKSAQIIPTRLTFVDIAGLVRGASKGEGLGNQFLAHIREVDAVA
                     YVLRCFEDPDITHVEGRVDPLGDAEAVETELMLADLESLERRRIPLEKKAKSGEKEAK
                     LQLAVIDKALALLREGKPARLAELTPEEEPLFRQLQLLTAKPVLYVCNVDEASAATGN
                     EYSRKVEERARAEGAGCVVISAKIEAELAGLSPEDRQSYLAELGLEEPGLNRLIREGY
                     RLLGLITFFTAGPKETRAWTVPAGTKAPKAAGVIHSDFEKGFIRAETIAYEDYVACGG
                     EAGAREAGKMRLEGKDYVVQDGDAIHFRFAT"
BASE COUNT          478 a          767 c          711 g          468 t
ORIGIN      
        1 gatgagcctg aaaactcgaa cgtgtcttta ttaatagagc gctcgcaccg cgagtaatcc
       61 cttctgcatg cgtgcctgat ttcgtcgacg taggctcgcg cgccgagaat ttcggccgcg
      121 cgaaaagatg ccgcgcgcgg gccttgccca gcccgcgctt tcgccggatt cagcccctca
      181 caccaagcaa aaactgcgcg aaattgacca ctttgtcggc agattcgcgc cataaactcg
      241 ccaatctcaa gtgttcgatg agcaacgcga tgcagaaaca aagaaaaggg gcctcacgtg
      301 ttgagtatcg gaactgggtt atatcagtcg tcttctgttt ctggactgag gattataacg
      361 tccgccgtca tcttttctct cgcaacactt gctccggcgt acgcatttcc aaaactcagt
      421 gatccaacaa ctgtccctgc agagccgaag gaacagaatg cgccggagac agaagagtcc
      481 acgcagcccg agtcgaatat cgacgtgtac ctgtacaggc tgatgatggc cgaatcggcc
      541 ggcaagccgc acgcgaagaa cccgcgctca tcggcactcg gtccttatca gttcatcaac
      601 tcgacgttca tcgaagtcgt cgacaagcat ttcgctgacg aggtccaagg aaagagccgc
      661 tcagaaattc tggctctgcg gaccgacatg gagtttgcac gtcgcgcggc aattgcgttc
      721 aacaacgaca acgcgaagta cctcgaagaa cgcggcgtga agcccacgtt cgctcacctt
      781 cggctgagcc acctgctcgg gcctgccgcg gctgtgaagg tgcttcgggc gaaacccaac
      841 actcgcctca cggccctcct gccttcgtcc gtgatccgcg caaatccgtt catgcggcgc
      901 atgacggtgg ctatgctggt ccgctggtcg gagcgcgagg tcggcgtggg tgtctggtcg
      961 actccgagcg agatcgccaa atccgatgag agcgaatctt ccgcgaccga ggccaagctc
     1021 gaagaggccg tggatgcacc ctcggagaag gccgagctgg aggttgccga agacaacaac
     1081 cgcgctctgt gaagcgcgcc gaacgccact ctcccccaaa ggtgttcagg ttccgccctc
     1141 ctccgcgtgg gaggagggcg ttttgatttg agctttctgc gtcaggtggc aaaccggaaa
     1201 tggatcgcgt cgccgtcttg gacgacgtag tccttgcctt ccagccgcat cttgccagcc
     1261 tcccgcgccc cggcttcacc gccacaagca acgtaatcct cataggcgat ggtttcggcg
     1321 cgaatgaacc ccttctcgaa atcggaatgg atgacaccag ccgccttggg cgctttggtg
     1381 ccggcgggca cggtccaggc ccgcgtctcc ttcggcccgg cagtgaaaaa cgtgatgaga
     1441 ccgagtagcc ggtacccttc gcggatcagc ctgttgaggc ccggttcctc gagtccgagc
     1501 tcggcgagat aggactgccg gtcttcgggc gaaaggccgg cgagctccgc ctcgatcttg
     1561 gcggagatta cgacgcaacc agcgccttcg gccctcgcac gctcctcgac cttgcgtgag
     1621 tactcgttgc cggtagctgc cgaggcttcg tcgacgttgc agacgtaaag caccggcttt
     1681 gcagtcagca gctgaagctg ccggaacaac ggctcctcct cgggtgtcag ctcggccagc
     1741 cgcgcaggct tgccctcgcg caaaagcgcc aacgccttgt cgatgacagc gagctgaagt
     1801 ttggcttcct tttcccctga cttggctttc ttctccagcg gaatgcggcg gcgttcgagg
     1861 ctttcgaggt cggcaagcat cagctccgtc tcgacggcct cggcatcacc caagggatcg
     1921 acgcggccct ccacgtgggt gatgtcaggg tcttcgaagc agcgcagcac ataggcaacg
     1981 gcatcaacct cccggatatg ggccaggaac tggttgccca gcccctcccc tttcgaggcg
     2041 ccgcggacaa gaccggcgat gtccacgaag gtaagccgcg tcggaatgat ctgcgcgctc
     2101 tttgcgatcg acgccagctt ctcaaggcgc gggtcgggca cgccaacttc gcccacgttc
     2161 ggctcgatgg tgcagaacgg gtagttggcc gcttccgcag ccgcggtttg agtcagcgca
     2221 ttgaagaggg tggacttgcc gacattcggc aggcccacga tgccgcattt gaagcccatg
     2281 aaaaccgatc ctgtccggga aagcccgccc ggcgaacccg gtcaggccct gagcatctaa
     2341 gctttggggg cgatcttgaa aacccctccc ggtgccgcat gagcctgatg caacggcggc
     2401 gtcaattgcg cccggctccc tatt
//
LOCUS       DASUMG010000115         5156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_175711, whole genome shotgun sequence.
ACCESSION   DASUMG010000115 DASUMG010000000
VERSION     DASUMG010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175711"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFX46_01850"
     CDS             complement(<1..412)
                     /locus_tag="VFX46_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599359.1"
                     /translation="MKITIPDFCLVAIVAPNASSAEALAQRHFHPSEIVSLTSQMWPK
                     LAADRLVRRELVAIEASEASIQKQIDLVRLAKTFYAQPVAIVLCDPATRSDLRKWRSF
                     ESTGFRPAHAVLPWQVAELEIERERLPSDLRHEHG"
     gene            complement(491..1870)
                     /gene="glcF"
                     /locus_tag="VFX46_01855"
     CDS             complement(491..1870)
                     /gene="glcF"
                     /locus_tag="VFX46_01855"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HEX5599360.1"
                     /translation="MQTNFTAAQLQDPRTQEIDRILRRCVHCGLCTAVCSTYVLLGDE
                     RDSPRGRIYMMKDMFERGGPASPEVRYHVDRCLSCLSCMTTCPGGVDYMHLVDHARAY
                     IEQTSQRPLKERLLRRLLAEIVPYPDRFRLALRAAPLGRPFQKLMRRFGLKELAAMLD
                     LAPKRGIRSPKYSGPGTAITQAERKGRVIMLAGCAQQVLRPEINDATIRLLARRGVDV
                     EVAAGAGCCGALVHHMGREEEAIAFAKRNVDAWWKVMSRGPIDAVVINASGCGTMVKD
                     YGHLLAGIEGYAQRAQKIAEMARDVTEFLAEYDLGPPKRWSSLRVAYHSACSMQHGQQ
                     ITEEPKQLLRDAGFTVLDIPEGHICCGSAGTYNILQPELAQQLRDRKTANIASVKPDL
                     VAAGNIGCITQLATSLDVPIVHTVELLDWAYGGPVPRGLEGYKQFVSDVPEPEMPRMK
                     RTLQDLLRA"
     gene            complement(1901..3121)
                     /gene="glcE"
                     /locus_tag="VFX46_01860"
     CDS             complement(1901..3121)
                     /gene="glcE"
                     /locus_tag="VFX46_01860"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HEX5599361.1"
                     /translation="MPTVRPGAEWELANLVVAATERKRPIEVMGAGTKQGVGRPIQSA
                     LTISTSALRGITLYEPTELVMSARAGTPVIEVEAQLAARGQMLPFEPIDLGPALGAEA
                     GRGTIGAVFATNLSGARRVATGSARDHLLGVRAINGRGETFKSGGRVLKNVTGYDVAR
                     GLAGSWGTLAILTEVTFKVVPKPERTATLVLFGLTDELASEVMCQAMGTPFEVSGAVH
                     LPLAMARRLRTPELRDPGKAVTAIRLENLANFVDYRSAKLREHLAPYGSVEVFGDDVS
                     LAFWGELRQLSVLQEERAKPLWRVSTKPTAGPKVVDAVRRHMAVEAFYDWSGGLIWLE
                     VPESADAGATDIRRITAQYGGHATLMRADPSVRAAIDVFQPLDPGVERLTRGLKAAFD
                     PAGILSPGRMYASL"
     gene            complement(3134..4582)
                     /locus_tag="VFX46_01865"
     CDS             complement(3134..4582)
                     /locus_tag="VFX46_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5599362.1"
                     /translation="MSSIQLPEPSPEILARRDAIIADLKALVGAGAVVSDEDGRRAYE
                     TDGLTAYRKMPLAVVLPQTTEEVSAVLRYCHQNGIKVVARGAGTSLCGGALPAEDAVV
                     VGVSKMNRVLEVDYANRIAVVQTGITNLAVSNAVSKDGFFYAPDPSSQLACTIAGNLA
                     MNSGGAHCLKYGVTTNNVLGAKLVLMDGEIIEIGGAHLDAPGYDLLGLIIGSEGQLGI
                     VTEATVRILRSAENARPMLLGFESSEAAGACVSAIIGSGIIPVAIEYMDKPAIEVCEA
                     FAKAGYPLDVEALLIVEVEGSEEEIQDLLARLVKIAEPFNPKTVKVSSSAAESAKIWR
                     GRKSAFGAIGRISEYYCMDGTIPLKQLPQVLTRISEICGSHGLKVANIFHAGDGNLHP
                     LILYNANDPQEVEKAELAGAEILKLCVEVGGCLTGEHGVGIEKRDLMRHQFSETDLLV
                     QMRIKSVFDPDWLLNPAKVFPLAERVMLDNAA"
     gene            4845..>5156
                     /locus_tag="VFX46_01870"
     CDS             4845..>5156
                     /locus_tag="VFX46_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX5599363.1"
                     /translation="MHSRGLIGLVFLVAALSPAAAQDAENGEQVFRKCRACHQVGDGA
                     KNTVGPQLNDIVGRVAGGVDGFNYSKANRKAAEEGLVWTEENLMAYLENPRKFMPGTK
                     MA"
BASE COUNT          981 a         1698 c         1545 g          932 t
ORIGIN      
        1 ggccatgctc atgccgtagg tccgaaggca accgctcgcg ctcgatctcg agctccgcga
       61 cctgccatgg tagaaccgcg tgcgctgggc gaaagccggt cgattcgaaa gaacgccact
      121 tacggaggtc tgagcgggtt gccgggtcac agaggacgat cgccacaggt tgagcgtaga
      181 aagtcttggc gagccggacc aggtcgatct gcttctggat gcttgcctcg ctggcctcga
      241 tggccaccag ctcgcggcgg accagccggt ccgctgccaa tttcggccac atctgcgaag
      301 tcaggctgac gatctcgctg ggatggaagt gccgctgggc gagcgcctcg gcactggagg
      361 cgttaggcgc aacgatcgcg acgaggcaga agtccggaat ggttatcttc accggcaggg
      421 aaacccctcg ggagacttga actcgaccaa ctcgggagat cgcgcggcgg ttcgccaaca
      481 ccacgcgtgc tcacgcacgc aataagtcct gaagagtccg tttcatcctt ggcatctccg
      541 gctccggcac atcgctgacg aattgcttat agccttccag cccgcgtggc accgggccgc
      601 cataggccca atccagcagc tcaaccgtat gcacgatcgg gacatccaag ctcgttgcga
      661 gctgcgtgat gcagccgatg ttaccggcgg cgaccaggtc tggcttcacg gacgcaatgt
      721 tagcggtttt cctatcccgt aactgctggg ccagctcggg ttgcagaata ttgtaggtgc
      781 cagcagaccc gcagcagatg tgcccctcgg gtatgtcgag aaccgtgaag ccggcatccc
      841 gaaggagctg ctttggctcc tcggtgatct gttggccgtg ttgcatggag caggccgaat
      901 gataggcgac gcgcaacgac gaccagcgct tgggcggccc cagatcgtac tccgccagga
      961 actcggtgac gtcccgcgcc atctccgcga ttttctgggc gcgctgcgcg tagccttcga
     1021 tcccggcgag cagatgcccg taatccttca ccatcgtccc gcagcccgag gcattgatga
     1081 cgacggcatc gatcggcccg cggctcatca ccttccacca cgcgtcgacg ttccgcttgg
     1141 cgaaggcaat tgcctcctcc tcacgtccca tgtggtgcac gagcgcgccg cagcatccgg
     1201 ccccggcggc aacctccaca tccacccccc ggcgggctaa cagccgaatg gttgcgtcat
     1261 tgatttcggg tcggagcacc tgctgggcgc agcccgccag catgataacc cgccccttcc
     1321 gctctgcttg cgtaatcgcc gtaccagggc cgctgtattt gggcgaacgg atcccgcgct
     1381 tcggcgccag atccagcatt gccgccagct ccttcagccc gaaccggcgc atgagcttct
     1441 ggaatggacg tccgagcggc gctgccctta acgcgagccg gaaacggtcc ggatacggca
     1501 caatctcggc gagcagcctc cgcagcagcc gctccttcaa tggacgttgg ctcgtctgct
     1561 cgatgtaggc gcgggcatga tccacgaggt gcatgtagtc gacgccgcca gggcaggtcg
     1621 tcatgcagga caggcacgac aggcagcggt ccacatggta gcggacctcc gggctcgccg
     1681 gccctccgcg ctcgaacatg tccttcatca tgtagatgcg gccgcgcggc gagtcccgct
     1741 cgtcgccaag cagcacatat gtcgagcaaa cggccgtgca gaggccgcaa tgcacgcacc
     1801 gcctgaggat ccggtcgatc tcctgggtgc gcgggtcctg cagctgtgct gccgtgaagt
     1861 tcgtctgcat cgtgcctgac cgcccctttg gcggatggat ctaaagagaa gcgtacatgc
     1921 gaccggggct cagaatgccc gccggatcga aggcggcttt cagtccgcgg gtcagccttt
     1981 caacgccggg gtcgagcggc tggaagacat cgatcgcggc gcggacgctc ggatcggcgc
     2041 gcatcagcgt tgcatggcct ccatactgag cggtgatccg gcgaatgtcc gtagcgccgg
     2101 catcggctga ctccggcacc tccaaccaga tcagccctcc cgaccaatcg tagaacgctt
     2161 ccaccgccat gtggcgcctg accgcgtcga cgaccttggg cccggccgtc ggcttggtcg
     2221 acacgcgcca cagcggcttt gcgcgctcct cctgcaaaac agacagctgg cgcagctcac
     2281 cccagaatgc gagtgacaca tcgtcgccga acacctcgac cgatccgtat ggtgccaagt
     2341 gctcgcgaag ctttgcgctg cggtagtcga cgaaattcgc cagattttcc aatcgaattg
     2401 ccgtcaccgc cttgcccgga tcgcggagct ctggcgtccg caaccgccgg gccatagcca
     2461 gcggcagatg cacagcgccg ctcacctcga agggcgtgcc catagcttgg cacatcacct
     2521 cggaagcaag ctcgtccgtc aatccaaaca ggacgagggt ggccgtgcgc tccggcttcg
     2581 gcaccacctt gaacgtcacc tcggtcaaaa tcgcaagcgt gccccagctt ccggccaacc
     2641 cgcgcgcaac gtcgtatccc gtgacattct tcagcacgcg accgccggac ttgaacgtct
     2701 cgccgcgccc gttgatcgcc cgaacaccaa ggagatgatc gcgagcggaa ccggtcgcca
     2761 cacgccgagc tccggacagg ttcgttgcga acaccgcgcc gatggtgccg cgccccgcct
     2821 cggcgcccag cgccgggccg agatcaatcg gctcgaacgg cagcatctgc ccccgggctg
     2881 cgagctgagc ctcgacctct atcaccggcg tgccagcgcg cgccgacatg acaagctcgg
     2941 tcggctcata cagcgtgatg ccccgcagag cactggtcga gatagtgagc gctgattgaa
     3001 tgggccggcc gacgccctgc ttggtgccgg cacccatgac ctctataggc cttttccgct
     3061 cggtggcggc aaccacgagg tttgccagct cccactccgc tccgggtctg accgtcggca
     3121 caggtcgcac tccttaggcc gcgttgtcga gcatcacgcg ctcggccaac gggaaaactt
     3181 tcgccggatt gagtagccag tccgggtcga acaccgattt tatgcgcatc tgcaccagca
     3241 ggtccgtctc gctgaactgg tggcgcatca gatctctctt ttcgatgccc acaccgtgct
     3301 cgccggtcag gcagccgccc acctcgacgc acagcttcag aatttcggcg ccagccagtt
     3361 ccgccttttc cacctcttgt gggtcgttcg cattgtagag aatcagcggg tgcaggttgc
     3421 catcgccagc atggaagatg ttcgcgacct tgaggccatg gctgccgcag atctccgaga
     3481 tgcgtgtcag cacctgcgga agctgcttca gcggaatggt accgtccatg cagtagtact
     3541 cggagatacg gccgatagca ccgaatgcgg acttgcgccc tctccaaatc ttcgcgctct
     3601 ccgccgcact gctgctcacc tttaccgtct tcggattgaa cggctcggca atcttcacga
     3661 ggcgcgcgag cagatcctga atttcctcct ctgagccttc gacctcgacg atcagcaacg
     3721 cctcgacatc gagcgggtag cccgctttgg cgaaggcctc gcacacctcg atcgccggct
     3781 tatccatgta ttcgatcgca acgggaatga tgcccgagcc gatgattgcg gagacgcaag
     3841 cacccgctgc ctcgctcgac tcgaagccga gcagcattgg gcgggcgttc tcggccgaac
     3901 gcaggatgcg caccgtcgcc tcggtcacga tgccgagctg cccttccgaa ccgatgatca
     3961 agcccagcag gtcgtacccc ggagcatcga gatgagcgcc cccgatctcg atgatttcgc
     4021 catccatcag cacaagcttg gcgccgagca cattgttcgt cgtcacgccg tacttgaggc
     4081 aatgtgcgcc gcccgaattc atggcgaggt tgccggcaat cgtgcaagcg agctggctgg
     4141 aaggatcggg cgcataaaag aatccgtcct tgctcaccgc attcgagact gcgaggttgg
     4201 tgatgccggt ctgcacaacg gcaatgcggt tggcgtaatc gacctccagc acccgattca
     4261 tcttcgacac gccgaccaca acggcatcct ccgccggcag agcgccgccg cagagcgagg
     4321 tacctgcgcc tcgcgcaaca accttgatgc cgttctgatg acaatagcga aggactgcgc
     4381 tgacctcctc ggtcgtttgc ggcagcacga cggcgagcgg cattttccgg taagccgtca
     4441 gcccatccgt ctcataggca cgccggccat cctcatcgct gactacggcc cccgcgccga
     4501 caagagcctt caggtcagcg atgatcgcgt ctcggcgggc gagaatttcc ggactgggtt
     4561 ccggcagctg aatggacgac attcgagcct cgcgtcaatg tagcgacgaa attgccacca
     4621 gggctcctaa ttggcacggc tgcggcagaa ggggcaacct ggtcgcgttg cgatcaccgt
     4681 ttatgtgggt catgactcgg tcgttggcag agcggcagaa acgtccgctg ggcgcagcgc
     4741 cgccatgctt cgacgaaagc tgggccaacc gaactcgtta ccgcaagtac aggtatctag
     4801 cggtatccac cggcttctga acgactgata gaggaaacgg aacgatgcac tcaaggggat
     4861 tgataggatt ggtcttcctc gtggctgcac taagcccggc cgctgcacag gatgctgaaa
     4921 acggcgaaca ggtcttcagg aaatgccgtg cgtgccatca ggtcggagat ggcgccaaga
     4981 atacagttgg tccccaactc aacgacatcg tagggagggt tgcagggggg gtcgatggtt
     5041 tcaactactc caaggccaac agaaaggcgg ctgaagaagg tctcgtttgg accgaggaaa
     5101 acctgatggc ctacctcgag aacccaagaa agttcatgcc cgggaccaag atggcc
//
LOCUS       DASUMG010000116         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_657778, whole genome shotgun sequence.
ACCESSION   DASUMG010000116 DASUMG010000000
VERSION     DASUMG010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657778"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..616)
                     /locus_tag="VFX46_01875"
     CDS             complement(167..616)
                     /locus_tag="VFX46_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX5599364.1"
                     /translation="MTVPSSIAHAVQQTQEWLKELRDNGGLASEAEAYAVLRAVLHQL
                     RDRLTLAEAVDLGQQLPLIIRGIYYEGWRPSHAPEKIRTKQKFLDQITLRLLPHQIPP
                     EPAVRDVFALLAHHLDPGEIADVIDQLPAEIKQLWPETARTFRARAG"
     gene            748..>3286
                     /gene="fdhF"
                     /locus_tag="VFX46_01880"
     CDS             748..>3286
                     /gene="fdhF"
                     /locus_tag="VFX46_01880"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX5599365.1"
                     /translation="MTEKITFELDGKLVEANEGETIWQVAQRYGTTIPHLCWNPEPGY
                     RADGNCRACMVEIEGERVLAASCIRKPTPGMKVKTASERARKSREMVFELFIADQPKK
                     EESHDPASKFWNWVEAMGISTTSRYPRSDMPAPDNSHAAIAVNLDACIKCGLCVRACR
                     EVQMNDVIGMAYRGFDSKPVFDFDKGMGDSTCVACGECVQACPTGALMEANLLDASGK
                     RVAFEEKSVDTVCPYCGVGCQTKVHVKGDRILYVDGRNGPANENRLCVKGRFGFDYIH
                     HPDRLKKPLIRREDAPKRGDIVVDPQNPWDVFREATWEEALEKAAAGFRRILERDGGR
                     ALAGFGSAKGSNEEAYLFQKLIRQGFGTNNVDHCTRLCHASSVAALMEGLNSGAVTAP
                     FTAAKDADCIIVIGARPAENHPVAATYLKNAAKRGANLIVMDPRGQSQGIARYASHVL
                     QFKPGRDVALLNAMLNVIITEGLADVQYVQAHTDGFEDLKARIVDFTPERMEPVCGIP
                     AATIREVARLYARSERSIIFWGMGVSQHTHGTDNARCLIALALVTGHIGRPGTGLHPL
                     RGQNNVQGASDAGLIPMVFPDYKSVEDAKIRSMYEELWGRSLDPKRGLTVVEIMHAIH
                     DDQIKGMYIMGENPAMSDPDVAHARAALAHLEHLVVQDIFLTETAWHADVVLPASAHA
                     EKWGTYTNTNRQVQIGRPVLNPPGEARQDWELIQEIAQRIGLDWNYRDVSEVFTEMAS
                     VMPSLKNITWDRLLREDAVTYPCDAPDKPGNEIIFATSFPTATGRARIVPADLLPPDE
                     LPDDEYPLVLTTGRLLEHWHTGAMTRRASKLDALEPEAIAGLNPKEMN"
BASE COUNT          627 a         1020 c         1065 g          574 t
ORIGIN      
        1 aggtgcgttt tgctcacctc aaggcgcacc tcgcgacggc gccgttgctt actgccaagc
       61 agcgccaccg gtacgcagaa ctgcgcggat actcaggccc ggacagtgga caccatcgtc
      121 atcatcagag gcattgatag gtccagtgac ttttcctttg ccgatatcag ccagcacgcg
      181 cacggaaggt ccgcgctgtt tcgggccaca gctgcttgat ctcggccggc agctgatcga
      241 tcacgtccgc aatctcgccg ggatcgaggt ggtgggccaa aagggcgaac acatcgcgga
      301 cggccggctc cggcggaatc tgatgcggca gcaggcgcag cgtgatctgg tcgaggaact
      361 tctgcttggt gcggatcttt tccggtgcat gagacggccg ccacccttcg tagtagattc
      421 cgcgaatgat gagcgggagc tgctggccaa gatcgacggc ttcggccaga gtcaggcggt
      481 cgcgaagctg gtgaagcacg gcgcggagca cggcataggc ctccgcctcg ctcgcaagtc
      541 cgccattgtc gcgcagctct ttcagccatt cctgcgtctg ttgcaccgca tgggctatgc
      601 tagagggcac tgtcatcgtc atgactccct gggaaatgtc ccgggcaaga ttgtcggacg
      661 cgttaggcgc gtcgtgaatg cgccagatca aattggcgcg agggcaaaac gcacgaacag
      721 cggcacaggt tgggccaggg gttggatatg accgagaaga tcacgttcga gctcgacggc
      781 aagctcgtcg aggccaacga gggcgagacc atctggcagg tcgcgcagcg atacggcacg
      841 accattccgc acctgtgctg gaacccggag ccgggctacc gggccgatgg caactgccgg
      901 gcttgcatgg tggagatcga aggcgagcgt gtgcttgccg cctcctgcat ccgtaagccg
      961 accccgggca tgaaggtgaa gaccgccagc gagcgcgccc gcaagagccg cgagatggtc
     1021 ttcgagctct tcatcgcaga ccagccgaag aaggaggaga gccacgaccc tgcttccaag
     1081 ttctggaatt gggtcgaggc gatggggatc tcgaccacga gccgctatcc acgttccgac
     1141 atgccggcgc cggacaactc acatgcggcg atagcggtca atctcgacgc gtgcatcaag
     1201 tgcggcctgt gcgtgcgcgc ctgccgcgag gtgcagatga acgatgtcat cggcatggcc
     1261 tatcgcggct tcgactccaa gccggtgttc gacttcgaca agggcatggg ggactcgacc
     1321 tgcgttgcgt gcggcgagtg cgtacaggct tgcccgactg gcgcgctgat ggaggccaac
     1381 ctgctcgatg cgtcgggcaa gcgcgttgcg ttcgaggaga aatccgtcga cacggtttgt
     1441 ccgtactgcg gtgttggctg ccagaccaag gtgcacgtca aaggcgaccg gattctctat
     1501 gtcgacggtc gtaacggtcc ggccaacgaa aatcggctct gtgtcaaagg gcgcttcggc
     1561 ttcgattaca tccatcaccc tgaccggctg aagaagccgc tgatccgccg cgaagacgca
     1621 cccaagcgcg gtgacatcgt tgtcgacccg cagaaccctt gggacgtgtt ccgggaggca
     1681 acctgggagg aggcgctgga gaaggccgcc gccggcttcc gtcgcattct tgagcgtgac
     1741 ggtggcaggg cgcttgcggg cttcggttct gcaaaaggct cgaatgaaga agcctatctg
     1801 ttccagaagc tcatccgcca gggcttcggc accaacaacg tcgatcactg cacgcgcctg
     1861 tgccacgcct catccgtggc cgcgctgatg gaggggctca actcgggtgc cgtcaccgcg
     1921 ccgttcacgg cggcaaagga cgccgactgc atcatcgtca ttggcgcgcg gccggcggag
     1981 aaccaccctg tcgctgcaac ttatctaaaa aatgccgcca aacgcggggc caatctgatc
     2041 gtcatggatc cgcggggcca aagccagggt attgcgcggt acgccagcca cgtcctgcag
     2101 ttcaagccag gtcgcgatgt ggcgctgctc aacgccatgc tcaacgtgat catcacggag
     2161 ggactggcgg atgtgcaata cgtgcaagcg cacaccgacg gctttgagga cctgaaggcc
     2221 cggatcgtgg acttcacgcc ggagcggatg gagcccgtct gcggtattcc ggcggcgacg
     2281 atccgcgagg tggcgcggct ttacgcgcgg tccgagcgct cgatcatctt ctggggcatg
     2341 ggtgtctctc agcacaccca cggcactgac aatgcccgct gcctgatcgc gctggcgctc
     2401 gtcaccggcc acatcggccg gccgggcacg ggtctgcacc cgctacgtgg gcagaacaac
     2461 gtgcagggcg cgtccgatgc tggtctcatt ccgatggtgt tcccggacta caagtccgtg
     2521 gaggacgcca agatccgcag catgtacgag gagctgtggg gccgttcgct cgatccgaag
     2581 cgcggcctga cggtcgtcga gatcatgcac gcgatccacg acgaccagat caagggcatg
     2641 tacatcatgg gcgaaaaccc agccatgtcc gatccggacg tggctcatgc ccgcgcagcg
     2701 ctcgcgcacc tcgagcatct cgtcgtgcag gacatcttcc tcacggagac ggcttggcac
     2761 gccgatgtcg tccttccggc atcggcgcat gcggagaagt ggggcactta caccaatacc
     2821 aatcgtcagg tgcagattgg ccgcccggtg ctcaatccgc ctggtgaggc gcggcaggac
     2881 tgggagctca tccaggagat agcgcagcgc atcggcctcg actggaacta tcgcgacgtg
     2941 tccgaggtgt tcacggagat ggcgagcgtg atgccatccc tcaagaacat cacctgggac
     3001 cggctgctcc gcgaggacgc cgtcacctat ccgtgcgacg cgcccgacaa gccgggcaat
     3061 gagatcatct ttgccacctc gttcccgacc gctacgggac gcgcccggat cgtcccggca
     3121 gaccttctgc ctcctgacga gttgccagac gatgagtatc cgctggtcct cacaacgggc
     3181 cggctgctcg agcactggca tacgggtgcg atgacgcgcc gggcgagcaa gctcgatgcg
     3241 cttgagcccg aggcaatcgc tggtctgaac ccgaaggaga tgaacc
//
LOCUS       DASUMG010000117         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_256130, whole genome shotgun sequence.
ACCESSION   DASUMG010000117 DASUMG010000000
VERSION     DASUMG010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256130"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..825
                     /locus_tag="VFX46_01885"
     CDS             79..825
                     /locus_tag="VFX46_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599366.1"
                     /translation="MKKIFALAWAMSLLALIAPTKLAAGDSDPRVPPGTDPGGVAVAV
                     ISTGIDYTQPNIAARLARDGEGEIIGWDFADDDAFPFAPAESDGNGTAFASVLLADAP
                     GARLVPLRIDYERPATFFLAFAFIDQTPARVVVISSDGFGPEHRGSIHQTASQFDRLL
                     IIMARDEEPDGEEDDVANVLIVPTEIANGPDWDNRPLTEPNPVQSSRGLSAVARAAAV
                     ALEIAESEPEWDGASLKRAVLARLASHRSQ"
     gene            complement(933..1958)
                     /gene="moaA"
                     /locus_tag="VFX46_01890"
     CDS             complement(933..1958)
                     /gene="moaA"
                     /locus_tag="VFX46_01890"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX5599367.1"
                     /translation="MVVQETRVAPAPMIDPFGRHVTYLRVSVTDRCDFRCVYCMSEHM
                     TFLPKKDLLTLEELDRLCSAFIAKGVNKLRITGGEPLVRKNIMWLFRSLSRHLESGAL
                     EELTLTTNGSQLPRYASELAACGVKRVNVSLDTLKPDRFRAITRWGDIQQVMDGLAAA
                     EKAGLAIKINAVALRGVNEDEIEDLLRFAHGRGYDLTLIETMPLGDIDGDRTEQYLPL
                     SIVRARLMERFTLEDIPYRTGGPARYVRVAETGGRLGFITPMTHNFCESCNRVRLTCT
                     GTLYMCLGQEDAADLRAPLRASADDELLYAAIDEAISRKPKGHDFVIDRRTRRPAVSR
                     HMSVTGG"
     gene            2186..2383
                     /locus_tag="VFX46_01895"
     CDS             2186..2383
                     /locus_tag="VFX46_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HEX5599368.1"
                     /translation="MSEPDRFNISLRKFLKQVGVTSQREIERIVREKDLAGKGRLKVR
                     MELTAEGTDLKHVVEEEIDLG"
     gene            complement(2447..3193)
                     /locus_tag="VFX46_01900"
     CDS             complement(2447..3193)
                     /locus_tag="VFX46_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX5599369.1"
                     /translation="MTDSRPDEKGNQQGPGAQIMRILSGAKREWPILLIFLLAAASVW
                     AFIALAGKVVANDTPAIDHQLLELFRDPEDPTRPIGSPWFREMMRDFTGLGGTGVLTL
                     ITLSAVGFFLLERKDSAAALLAVAVLGGVVLNNVLKIGFGRPRPDLAAYEPFLSSASF
                     PSGHSTMAAVVYLTIGALIARLHPDPRVKIYVLTLSVLITLIVGVTRVYLGVHWPTDV
                     LAGWAVGSAWALFCWLVALVLQRRNAIEQT"
BASE COUNT          730 a         1091 c         1148 g          703 t
ORIGIN      
        1 gacgatcggg ccgctcgggg tgcggcggga cattgagacc ggccgttcgt cttcgatccc
       61 ggctcagaag cgaagcgaat gaaaaagatt ttcgctctcg cgtgggcgat gtcgcttctc
      121 gccctgatcg cacctacgaa gttggcggct ggcgacagcg atcctcgtgt gccgcctgga
      181 acagatcccg gtggcgtcgc tgtggccgtg atttcgactg ggattgatta cacgcagccg
      241 aacattgccg ccaggctggc ccgagatggc gaaggggaga tcatcggctg ggatttcgcg
      301 gatgatgatg cgttcccctt tgcgcctgca gagtcggacg gaaatggaac cgctttcgcg
      361 agcgtattgc tggctgatgc acccggcgcg aggcttgtcc ccttgcgcat cgactatgag
      421 cggccggcga cgtttttcct tgcctttgcg ttcatagacc agactcctgc tcgcgtggtc
      481 gtgatatcga gcgacggttt cggacctgag cacaggggct caattcacca gaccgcctct
      541 caattcgatc gcctgctgat catcatggct cgtgacgagg agccggacgg tgaggaggac
      601 gatgtcgcga acgtgctgat agtccccacc gagattgcta acggtcctga ttgggataat
      661 cgtcccctca ccgagccaaa tccggtgcaa tcttcacggg gcttatcggc agtggcgcgc
      721 gccgctgcag tcgcgctgga aatcgcagag agcgagccgg agtgggacgg agctagtctg
      781 aaacgtgctg tgctcgcgag gcttgcgagt cacagatctc aatagcgagc gtcgctggca
      841 acagcgctat cgcggatacg ctggtccgcc tccgcaattc aaacctgaaa cgcccaaaat
      901 tctagagtgc cgatcaaact gcgatccagc gtctacccgc ccgtcacact catatgccgc
      961 gaaaccgccg gccggcgggt gcggcggtcg atgacgaaat catggccctt cggcttgcgg
     1021 ctgatggctt cgtcaatggc cgcataaagc agctcatcat cggcgctggc gcgcagaggc
     1081 gcgcgcagat cggccgcatc ctcttggcca aggcacatgt aaagggtgcc agtacaagtc
     1141 agccgcacgc gattgcagct ttcgcagaag ttgtgggtca tcggagtgat gaagccgagc
     1201 cggccgcctg tctccgccac ccgcacatac cgggcgggcc cgccggtgcg atagggtatg
     1261 tcctccagcg tgaagcgctc catcaggcgg gcgcgcacga tggaaagtgg caagtattgt
     1321 tccgtacggt cgccatcgat gtcgccgagg ggcatcgtct cgatgagcgt caggtcatat
     1381 ccgcgcccat gggcgaagcg cagcaagtct tcgatctcgt cctcgttcac gcccctgagc
     1441 gccacagcgt tgatcttgat ggccaacccg gctttctcgg cagccgccag cccatccatg
     1501 acctgctgga tgtcgcccca ccgggtgatt gctctgaagc ggtcgggttt gagcgtgtcg
     1561 agcgagacat tgacacgctt cacgccgcag gccgccagct cgctggcgta tctcggaagc
     1621 tgactgccgt ttgtggtgag cgtcagctcc tcgagcgcgc ccgactcgag gtgccgagaa
     1681 agggagcgga acagccacat gatgttcttg cgcaccagcg gctcaccgcc cgtgatcctc
     1741 agcttgttca cgcctttggc gataaaggcc gagcacagcc ggtcaagctc ctccagcgtc
     1801 agaaggtcct tcttcggcag gaaggtcatg tgctcggaca tgcagtaaac gcagcgaaaa
     1861 tcgcagcggt ccgtcacgga gacccgcagg tacgtcacgt ggcgcccgaa agggtcgatc
     1921 atcggggcag gggccacacg ggtctcttgc acaaccattc ggctacctca tgggtcgagc
     1981 agtgtccggt gtcgggaaat atcttacgcc taaaggcaag gtaccgcagc accaagattg
     2041 atcaagtgct tccaactacg tggcttgttc agttcgtcgc gatttgcgct actgcgcctg
     2101 cggcctcaac cactgtccag atcggttcag ggcgcctata cctatcataa cattgcgtcg
     2161 ctatagctgc ttacgagagg tcatcatgtc agagcctgat cggttcaaca tctcccttcg
     2221 gaagtttctg aaacaggtgg gcgttacgtc gcagcgcgag atcgagcgca tcgttcgcga
     2281 gaaggacctc gcgggcaaag gccggctcaa agtgcgcatg gagctgacgg cggaggggac
     2341 cgacctgaaa cacgtcgtcg aggaagagat cgacctcggc taggccagtg gagcttgggc
     2401 cgataggcct gtccgctgag tcattgtgaa aaaggacgtc tttggatcag gtctgctcaa
     2461 tcgcgtttcg ccgttgcagc accagtgcga cgagccagca gaagagggcc caggcggagc
     2521 cgaccgccca gcccgcaagc acatcggtcg gccagtgaac gccgaggtaa acgcgcgtga
     2581 cgccgacgat cagtgttatc agcaccgata aagtgaggac gtagatcttg acccgcggat
     2641 cgggatggag ccgggcgatc aatgcgccga tagtcaaata gactactgcg gccatcgtcg
     2701 agtgtccgct cgggaagctg gcagaggaga ggaacggttc gtacgcagcc aggtcgggac
     2761 gggggcgccc gaatcctatc ttaaggacgt tgttcaacac gacaccgccc aggacggcca
     2821 cggcgaggag agccgccgcg ctgtccttcc tttcgagcag aaagaagccg accgcgctca
     2881 acgtaatcag cgtgagcacg ccggtgccgc ccaatcctgt gaagtctcgc atcatctccc
     2941 gaaaccaagg cgatcctatc gggcgcgtcg ggtcctcggg gtcgcggaac agctcaagca
     3001 gctggtggtc gattgccggc gtgtcgttcg cgacaacctt gccggcgagc gcgatgaagg
     3061 cccagaccga tgccgctgcg aggaggaaaa tgagcagaat gggccactcg cgcttggcgc
     3121 cgctcaggat ccgcatgatc tgggccccag ggccttgctg gtttcctttt tcatcaggtc
     3181 gggagtcggt cataagttct ctgatccggt cgttagagtt tcggccgaat gagcgcgtgc
     3241 gttagaacgt ctactcaagc tagatctgtc ggctgccgtg aaaccggctt ggcaggaagg
     3301 tggccgagct tggcgcgcac tgtagtagtc ttccaccgca ctgcaagccg ctccgctaac
     3361 cagccgctca agcagggaac gcaaccgttc ccagtgctct tcgacccatt catcggcggg
     3421 ccagtcgaaa tagttccacg cactatgatg cgtattgagg accgaccaaa tgagctccgc
     3481 agcgcctgag cgcaaggagt ttcgaagctg acgaacagag cgcgcgtcag caaggctcgg
     3541 accccatgct atgcaagacc atgcgccgcg gatgcgcgtg gattgggcaa tccgctgcta
     3601 ctgatttttc acctgccagc ggttaaagga agccgtgcgg ctgctttgca ttccgtatcc
     3661 ggatgcccgg ga
//
LOCUS       DASUMG010000118         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1139602, whole genome shotgun sequence.
ACCESSION   DASUMG010000118 DASUMG010000000
VERSION     DASUMG010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139602"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VFX46_01905"
     CDS             complement(<1..1106)
                     /locus_tag="VFX46_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX5599370.1"
                     /translation="MPLNSRFDDPDKLVDTDRGFISREIFVDPDLYKEELEKVFTRAW
                     LFIGHESLIPKPGDFYTTRMGEESVILCRDKQGVVHAFLNSCRHRGMKVCRYESGNTS
                     LFTCPYHSWTYTTDGKLQGVPHFRTLYEGLDREANSLVSVARLAIYKGTIWATWDKDA
                     PDFVTYLGDAKIHLDQALDCRDGRSGGSEVIGVHKWIFPANWKFAAENFLGDTYHNPS
                     HRSVDLIGIGPSAAAGKKGRRDDELEKAQHVWISFPEGHGVHSAIQPEANEYIESFLD
                     QPVLDQYFRHCFEERKRRLGEQARLLPFVGTIFPNTSYHGRQPRGLCAWHPHSPTSTE
                     GWRFFLVDADAPQEAKDYLRRYYMRYSGPAGMTE"
     gene            complement(1160..>2209)
                     /locus_tag="VFX46_01910"
     CDS             complement(1160..>2209)
                     /locus_tag="VFX46_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5599371.1"
                     /translation="NRMVAPDLFESRPAGLARPRSAAPCWASVRSSIACLLAVLFAVF
                     ANTQGEAQESRRPVTIVVPFSPGTGPDIIARIIAEKLQPRLGQPVIVDNKTGASGSIG
                     SQQVMRAAPDGHTLMVTADPPFTANVSLMKAVIYDPVKGFTPIIEAAVGTLALVVHPS
                     LPANSIKELIAYAKGRPGEVNYASPGVGTPHHLAMEFFKLTAKIDLMHVPFRDSAGAI
                     SNLLGGHVSAMFLPVHVALPLAPDKVRILAVATKARTPLLPDQPTLDELGFPGFDADI
                     RYGVLGPAGMPPEIVAKYNSEISEILRSPDVTERLAKQGLRSAPDTPDAYAANIVQDL
                     AKWRNVVTDAKLSTD"
BASE COUNT          418 a          666 c          708 g          417 t
ORIGIN      
        1 tgctcggtca ttccggccgg gccggagtag cgcatgtaat aacggcgcag atagtccttc
       61 gcctcctgag gcgcatcggc atcgaccagg aagaagcgcc acccttcggt ggaggtgggg
      121 ctatgcgggt gccaggcgca gagcccgcgc ggctgtcgcc cgtgatagga ggtgttgggg
      181 aaaatcgtac ccacgaacgg caacagcctc gcctgttccc caaggcgccg tttgcgctcc
      241 tcgaagcagt gacggaaata ctgatcgagc acgggctgat cgagaaacga ttcgatgtat
      301 tcgttcgctt cgggctggat cgcgctatgt acgccgtgtc cttccgggaa actgatccag
      361 acgtgctggg ccttttccag ctcgtcatcg cggcgcccct tctttccggc cgcggcactc
      421 ggcccgatgc cgataagatc gacggagcgg tggctcgggt tgtggtaggt gtcgccgagg
      481 aagttctcgg ccgcgaactt ccagttcgcc gggaagatcc acttgtgtac gccgatcacc
      541 tccgatccgc cgctccggcc atcgcggcag tcgagcgcct gatcgagatg aatcttcgcg
      601 tcgccgagat aggtcacgaa gtcgggcgca tccttgtccc aggtggccca gatcgtcccc
      661 ttgtagatcg cgagcctggc aacggagacg agcgaattgg cctcgcgatc aagcccctca
      721 tacagcgtcc tgaaatgcgg cacaccctga agcttgccgt cggtggtgta ggtccagctg
      781 tggtaggggc aggtaaacag cgaggtattg ccggactcat agcggcacac cttcatgccg
      841 cgatgacggc acgaattcag gaacgcgtgc acgacgccct gcttgtcccg gcagagaatg
      901 acggactcct cccccattcg ggtcgtgtag aagtcgcccg gcttggggat aaggctctcg
      961 tgcccgatga acagccaggc gcgcgtgaaa accttttcca gctcctcctt gtagagatcc
     1021 ggatcgacga agatttcgcg gctgatgaag cccctgtccg tgtcgacgag cttgtcggga
     1081 tcatcaaagc ggctgttcag cggcatgatc ggttctccac attgttggcc gagcggccac
     1141 gcttggccgg tagcaaaagt cagtctgtcg agagcttggc gtccgtgacg acgttgcgcc
     1201 acttggcgag gtcctgaacg atattggccg cataggcgtc cggcgtgtca ggagcagacc
     1261 tcagcccctg cttcgccagt cgctccgtca cgtcgggaga gcgaaggatt tcgctgattt
     1321 ccgaattgta cttggcgacg atttcgggcg gcatgcccgc cggcccaagg acgccgtagc
     1381 ggatatccgc gtcgaaaccg gggaacccaa gctcgtccag cgtcggctgg tcaggaagca
     1441 gcggcgttcg ggccttcgtt gccacggcaa gaatcctcac cttgtccggc gcgagcggaa
     1501 gcgcgacgtg aaccggcagg aacatcgcgc tgacgtgacc gccaaggaga ttggaaatcg
     1561 cgcctgccga gtcgcggaac ggcacatgca tcaagtcaat cttggccgtg agcttgaaga
     1621 attccatggc gagatggtgc ggtgtgccga ccccgggcga ggcataattg acctctcccg
     1681 gacggccctt ggcgtaggcg atcagttcct tgatcgaatt cgccggcagg gacggatgca
     1741 cgacaagcgc aagcgttcca accgccgcct cgatgatggg cgtgaagccc ttaacagggt
     1801 catagatgac tgccttcatc aggctgacat tggccgtgaa cggcgggtcc gcggtcacca
     1861 tcagcgtatg gccgtccgga gcggcgcgca tgacctgttg cgatccgatg ctgccactgg
     1921 cgccggtctt gttgtcgacg atcaccggct gcccgagcct gggctgcaat ttttcggcaa
     1981 tgatgcgtgc aatgatatcg gggccggttc cgggcgaaaa cggcaccacg atggtaacgg
     2041 gacgccgcga ttcctgcgct tcgccttgcg tgttggcgaa cacagcgaac agcaccgcca
     2101 ggaggcaagc aatgctcgag cgcacggatg cccagcatgg cgcagcggac ctcggccgtg
     2161 cgagacctgc aggacgagat tcaaacagat cgggcgccac catccgatt
//
LOCUS       DASUMG010000119         3386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_658063, whole genome shotgun sequence.
ACCESSION   DASUMG010000119 DASUMG010000000
VERSION     DASUMG010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658063"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="VFX46_01915"
     CDS             <1..746
                     /locus_tag="VFX46_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX5599372.1"
                     /translation="RWLDADRESVREPEAFLRRVVTRLCIDHLRSARHRRETYIGPWL
                     PEPVIEPESEFDDITLPLMMALERLSPLERAAFLLHDVFGVSFDEIAETIGREPAACR
                     QLASRARNHVRAARPRFTLPKERGLELAAAFFAASRNGDMQRLRALLAADVAVYADGG
                     GKVPAAMRPIVGFDKVMQFQTSLAQFFAKNMSRLLRYSFINGLPGFVTIEHGGILQTT
                     ALQIDDGKITAIYVIRNPEKLQHLSCISH"
     gene            complement(806..2128)
                     /locus_tag="VFX46_01920"
     CDS             complement(806..2128)
                     /locus_tag="VFX46_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX5599373.1"
                     /translation="MLDKTDTKAAARRSVVVQSFTNSVLDPEAPMLGPVEDGGTIIAN
                     TAPGCWGPMITPRLRGGHEVTQPVYVEGAEPGDGIAIRIRDITVTSIATASGHDTSPS
                     GYCLGDPYVAARCPTCDEVWPETHLEGIGQNAVICDKCGNPVKPFEIVHGYTVVFDET
                     RQVGVTMPKAAAEEIARNAYHYAALPDKSVQHPILVFAPSDMPAVMVRMRPFLGQLGT
                     MPSIAMPDSHNAGDFGSFLVGAPHAYGITAEQLAEHKTDGHMDIDAVRPGAILIAPVK
                     VKGGGVYMGDMHAGQGDGEIAGHTMDVAGSVTLQVEVVKNYPIDGPVLFPLVEDLPPL
                     AKPFTAEERARAERLAQSWGMDTLEELAPISVIGTAANMNAAIDNGLQRAAKLLGMSV
                     PEVRNRATINGAIEIGRAPGVIQVTFLAPLSRLDAVGLGKYAREQYNL"
     gene            complement(2263..2338)
                     /locus_tag="VFX46_01925"
     tRNA            complement(2263..2338)
                     /locus_tag="VFX46_01925"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2303..2305),aa:Ala,seq:cgc)
     gene            2549..2848
                     /locus_tag="VFX46_01930"
     CDS             2549..2848
                     /locus_tag="VFX46_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEX5599374.1"
                     /translation="MIPLLLFIDYLLNLYTWVVIASVVFSWLIAFNVINPYNQFVRTV
                     WQALNAVTEPLLRPIRRYMPDLGGIDLSPVVLLLGIFFVRSVVIRGWLIGGLLSG"
     gene            2845..3186
                     /locus_tag="VFX46_01935"
     CDS             2845..3186
                     /locus_tag="VFX46_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HEX5599375.1"
                     /translation="MTASRTGDAICRAIPTGIAIRVRLTPRSQRDSIDGLEETAEGTA
                     LKARVRAVPESGAANAALERLVAGWLGVPKSSVEVIAGGKSRLKTVAVSGNAEALQRQ
                     LDAKLACLSGR"
     gene            3218..>3386
                     /locus_tag="VFX46_01940"
     CDS             3218..>3386
                     /locus_tag="VFX46_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX5599376.1"
                     /translation="MAARVIDGKAIAAEVRRSVAAEVEQLKNEKGLTPGLAVVLVGED
                     PASQVYVRNKAR"
BASE COUNT          613 a         1045 c         1089 g          639 t
ORIGIN      
        1 tccgttggct cgatgccgac cgcgagtctg tacgtgagcc ggaggccttt ctgcggcgcg
       61 tcgtgacccg gctctgcatc gaccacctga gatccgcgcg acaccggcgc gagacttata
      121 tcggaccgtg gttgccggag ccggtgatcg aaccggagag tgaattcgac gacattacgc
      181 ttccgctgat gatggcgctg gagcgattgt cgccgctgga acgtgccgcc tttctgctcc
      241 acgacgtgtt cggcgtcagc ttcgacgaga ttgcggaaac gattggtcga gagccggcgg
      301 cctgccgcca actcgcaagc cgcgcgcgga atcacgtgcg tgcagcgcgt ccccgcttca
      361 ccttgcccaa ggagcgaggt ctcgagctcg cggctgcatt cttcgccgcg tcccgcaacg
      421 gcgacatgca gaggcttaga gcactcctgg cggctgacgt cgcagtctac gccgacggtg
      481 gcggcaaggt gccggcagcg atgcgaccga tcgtcggatt cgacaaggtc atgcagttcc
      541 aaacctcgct ggctcagttt ttcgcgaaga acatgtcgcg attgctgcgc tacagcttca
      601 tcaacggctt gcccggattc gtaaccatcg agcacggcgg catcctacag accaccgctc
      661 tgcagatcga cgacggcaag atcactgcca tctacgtcat acggaatccg gagaagctgc
      721 agcacctgag ctgcatcagt cactgatcgg caatctcaat ggcgagcttc ccctccgggt
      781 atgaacggcg gagggaagca tgctttcaga ggttgtactg ctcgcgggcg tacttcccga
      841 gcccaaccgc gtcgaggcgg ctgagaggcg caaggaaggt cacttggatg acgccgggtg
      901 cccggccaat ttcgattgcg ccgttgatcg tcgcccgatt gcggacctcc ggaacgctca
      961 tgccgagcag tttggcggcg cgctgcaaac cgttgtcgat cgcagcgttc atgttggccg
     1021 ctgtgccgat aacggagatg ggtgcaagct cctcgagagt gtccatgccc cacgattgtg
     1081 cgaggcgttc tgccctcgcc cgctcttccg ccgtgaaagg tttcgcaagc ggtggcaaat
     1141 cctcgacgag cgggaagaga actggcccgt cgattgggta gttcttcacc acctccacct
     1201 gcaacgtgac gctgccggcg acgtccattg tgtgaccggc gatctcgccg tcgccctggc
     1261 cggcatgcat gtcgcccatg tacacgccgc cgcctttcac cttcacaggc gcgatcaaga
     1321 tggcgccggg ccggacggca tcaatatcca tgtgcccgtc cgtcttatgc tccgcaagct
     1381 gctcggccgt gatgccgtaa gcgtgcggcg ctccaaccag aaacgagccg aaatcgccgg
     1441 cattgtgcga gtcgggcatc gcgatggagg gcatcgtccc gagctgccca aggaagggcc
     1501 gcatccgcac catcaccgcc ggcatgtcgg acggggcgaa tacgaggatc ggatgctgca
     1561 cggacttgtc cgggagcgca gcgtagtgat aggcgtttcg cgcgatctcc tcggcagcgg
     1621 ctttcggcat cgtgacgccg acctgccggg tttcatcgaa aaccaccgtg tagccgtgca
     1681 cgatctcgaa aggctttacc gggttaccgc acttatcgca gatgacggcg ttctggccga
     1741 tgccttcgag gtgggtttcc ggccagacct cgtcacaagt cgggcagcgc gcggcgacgt
     1801 acggatcgcc caggcagtaa cccgagggtg aagtgtcatg tcctgaagcc gttgcaatcg
     1861 aggtgacggt gatgtcgcgg atgcggatgg caatgccgtc gccaggctcg gcgccctcca
     1921 catagacggg ctgcgtcacc tcgtgcccgc cgcgcagacg tggcgtgatc atcggccccc
     1981 agcaaccggg cgcggtattt gcgataatcg tcccgccatc ttcgaccggc cctagcatcg
     2041 gggcctccgg gtcaaggacg ctgttggtga agctctgcac gacgacgctg cggcgggccg
     2101 ccgctttggt atccgtcttg tccagcatgc cagtctcccg ttcggctctc attcatcgaa
     2161 cgtaaactac ggggacgccg cgaggacatc acatgatgct gatcaagacc gccagcggcc
     2221 aaggttagaa tgagattagc gggaaggcag atgccaggaa attggtggag ccgaggggaa
     2281 tcgaacccct gacctctgca gtgcgattgc agcgctctcc catctgagct acggccccaa
     2341 tcgcgcttgc tggcgcatgg gagctggcat ttaggagcga cccccgcggg ttgtcaagca
     2401 aaccggccgt cacgtcgggc ttgccgcagt agcgccctgc tgtggctttg ggccgacttg
     2461 gccaactatg agaagccact ccgcttgccc cacacgctcc ggttcgtcta cacctgtcgg
     2521 gcagcgaacc ggaggagacc cgcgcgcgat gatacctttg ctgctcttca tcgactacct
     2581 gctcaacttg tacacttggg tggtaatcgc cagcgtcgtg ttctcctggc tgatcgcgtt
     2641 caacgtcatc aatccctaca accagttcgt gcggacggtc tggcaggcgc tcaacgctgt
     2701 aaccgagccg ctgctgagac ccattcgacg ctacatgccc gatctaggcg gcatcgacct
     2761 ttcgccagtg gtgctgctgc tcgggatctt tttcgtccgc tcggttgtga ttcgcggctg
     2821 gcttatcggg gggctgctaa gcgggtgacg gcatcgcgca ctggggatgc gatctgtcgc
     2881 gcgatcccca cgggtatcgc gattcgggtg cggctgaccc cgcggtccca gcgcgacagt
     2941 atcgacggcc tggaagagac cgcagaaggc acggcgctca aggcgcgggt tcgagctgtt
     3001 ccggagagcg gcgcagcaaa tgccgcattg gaacggctcg ttgcaggctg gcttggtgtt
     3061 cccaagagca gcgtcgaggt gatcgccggc ggcaagtcgc gactcaagac cgtggcggtt
     3121 tcgggaaatg cagaggcgtt gcagcggcag ctcgatgcca agctcgcttg tctgagcggc
     3181 cggtgaaaat ccaagaattt gttgaaggaa agaaggtatg gcagcgcggg tgatcgacgg
     3241 aaaagccatt gcggcggagg tgcgccggtc ggtagcggcc gaggtcgagc agctgaaaaa
     3301 cgaaaagggg ctgacgccag ggctcgccgt cgtgctcgtg ggcgaggacc cggccagcca
     3361 ggtctacgtg cgcaacaagg cgcgcc
//
LOCUS       DASUMG010000120         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_417008, whole genome shotgun sequence.
ACCESSION   DASUMG010000120 DASUMG010000000
VERSION     DASUMG010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417008"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="VFX46_01945"
     CDS             complement(<1..789)
                     /locus_tag="VFX46_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5599377.1"
                     /translation="MTVLSRRTFVLSATAAAAAFGLKGPLAFIGRAEAMGPGSELPRF
                     AKFKVGDLEVIQLYDGVWQKPHDPNFIKNATVEETKKALREAGLTDAHVPITFTVTVV
                     RMGDRTIMFDAGTGGQMAPTAGLLMKESMEAAGLDPKSINTIVVTHFHPDHIFGLMAK
                     DTNAQIFPNAEIVVPATEYKYWTDAGVFSSLPEARHGLAKRVQATFPSWSNIRQVEED
                     SEVLPGIRSVASYGHTPGHTSYLISSGNDQLMVLGDVSNLPALFV"
     gene            complement(909..1601)
                     /locus_tag="VFX46_01950"
     CDS             complement(909..1601)
                     /locus_tag="VFX46_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX5599378.1"
                     /translation="MISVVIPTLNAEERLGPTLAALIPAAVDGLVREVIVADGGSTDR
                     TLDIADAAGAEIVRCEPGRGMQLMAGAEKARFPWLLFLHADTVLEPGWHDEARSFIDT
                     VEAKRVSPAAAVFRFSLDDTGLAPRIVEAGVAVRCTLFKLPYGDQGLLIPARLFREIG
                     GYKPLPLMEDVDIVRRLGRRRIVMLRSRAITSAARYRRDGYGRRIARNLSCLGLYLLG
                     TPVEKISRFYER"
     gene            1713..>2011
                     /locus_tag="VFX46_01955"
     CDS             1713..>2011
                     /locus_tag="VFX46_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5599379.1"
                     /translation="MSNTAKGRIQIAAGTLVQEERRRGRGARSNRSGRFEAEVREDFD
                     DGWGSLAELEAFKTEVRIERARNIITTNNSPDIGFDRSINPYRGCEHGCIYCYARP"
BASE COUNT          413 a          618 c          589 g          391 t
ORIGIN      
        1 cacgaagagc gccggcaggt tggagacatc acccaacacc atcagctggt cgttcccgga
       61 gctaatcaga tagctggtat ggccgggcgt gtgaccatag ctggcgaccg agcggatgcc
      121 gggcaacacc tcgctatcct cctcgacctg cctgatgttt gaccaactcg ggaaggtcgc
      181 ttgaacgcgc ttggccaacc cgtgccttgc ctcgggtaag gaggagaaca cacccgcatc
      241 cgtccagtac ttatattcag tggccggcac cacgatctcg gcgttgggga agatctgagc
      301 gttcgtgtcc tttgccatca gcccgaagat gtgatcgggg tggaagtgcg tcaccacgat
      361 cgtattgatg gatttgggat cgagaccggc ggcctccatg ctttccttca tcagcaaccc
      421 agccgtcggg gccatctgcc cgccggttcc ggcatcgaac atgatcgtcc ggtcgcccat
      481 gcggactacg gtgacggtga aggtgatcgg cacatgggca tctgtaagcc cggcttcacg
      541 cagagctttt ttcgtctcct caacagtggc gttcttgatg aagttcggat cgtgcggctt
      601 ctgccaaact ccatcgtaaa gctggatcac ctccagatcg cccaccttga attttgcaaa
      661 cctgggaagc tcgcttcccg gtcccatcgc ctcggcgcgg ccgatgaagg ccagtggtcc
      721 cttgaggccg aacgcagctg ctgcagctgt agccgacagt acaaacgtac gacgagacaa
      781 cacggtcata gcgactcctt ggacttgatc tctccgcatg acttgcacgg aaaatgtgtt
      841 cccggaaggt acgcgggctc tgctccaagg aaaagcggcc ggtgacgtct cacgcagttt
      901 tgaaggtctc atcgctcgta gaagcgagag atcttctcca ctggtgtccc caacaagtag
      961 agcccgagac aggaaaggtt tctagcgatg cgccggccat atccatcgcg gcggtatcgt
     1021 gcggcgctcg tgatcgcacg cgagcgaagc atgacgatcc gtctgcggcc gaggcggcgc
     1081 acgatgtcaa catcctccat cagcggcaaa ggcttatagc cgccgatctc gcgaaagagc
     1141 cgcgctggga tgaggaggcc ctggtccccg tacggcaact tgaacagagt gcaccttacg
     1201 gccacgccag cctcgacaat tcgaggcgca agcccggtgt catcgagcga gaaccggaag
     1261 accgccgccg ccggtgacac ccttttcgcc tcgacggtgt cgatgaagct tctggcttcg
     1321 tcgtgccaac ccggctccaa gaccgtgtct gcatgcagaa acagaagcca gggaaatcga
     1381 gctttttcgg cgcctgccat aagctgcatc ccccgccctg gctcgcagcg gacgatctcg
     1441 gctccggcgg catcggcgat gtccagcgtc cgatcagtcg agccgccatc cgccacaatc
     1501 acctcacgta ccaaaccatc cactgcggct gggatcagcg ccgcaagtgt cggcccaagg
     1561 cgctcctcgg cattgagcgt gggtatgacg acggagatca tgaacgcctt cttatgagga
     1621 aaggccgcga tctagaaggg cgtaagtgtt cttgatttgt tcttatgcct tggctattgt
     1681 ccacatgacc caaaggttgg gtagtgctcc cgatgtcgaa caccgccaag ggccgtattc
     1741 agatagcagc cggtactctc gtccaagaag aacgccgccg cggccgcggt gcgcgctcta
     1801 accggtccgg ccgctttgag gctgaggtac gggaggactt cgacgacggc tggggaagct
     1861 tggctgagct cgaagcattc aagaccgagg tgcgcatcga gcgcgcgcgc aacatcatca
     1921 cgacaaacaa cagcccggat atcggtttcg accgatcgat caatccctat cggggttgcg
     1981 agcacggctg catctattgc tacgcccggc c
//
LOCUS       DASUMG010000121         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_899231, whole genome shotgun sequence.
ACCESSION   DASUMG010000121 DASUMG010000000
VERSION     DASUMG010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899231"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFX46_01960"
     CDS             complement(<1..1059)
                     /locus_tag="VFX46_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEX5599380.1"
                     /translation="MTAKIEGDMGESRMGGARMGLAAAVAAVVLSGLPGGTATAETLL
                     QALTSAYKFNPRLDAERARLRATDEEVPRALSGYRPFIFGSADVGYQRSVSRGSGGTD
                     TRESNPRGYGIDISQPLFRGFRTLNTVREAEAVVRAQRANLHIIEQSVLLEAVIAYAD
                     VVRDQAVVRLRENNVNVLTRELRATEDRFAVGEVTRTDVAQAQARRAGAVSALDLARA
                     NLRTSRAAYERVIGHPPSNLTDPSVPEHLLPKSQQEALAIAEQENPNIVAALYREQAA
                     RHVVDRIWGELLPEVSVDASYDRRFGQSEVFDDVETTRVTGRLTVPIYQGGEVSARVR
                     QAKHTHVSRLQEVEQFRTE"
     gene            complement(1270..1935)
                     /locus_tag="VFX46_01965"
     CDS             complement(1270..1935)
                     /locus_tag="VFX46_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX5599381.1"
                     /translation="MVNFAVQRKNMVESQVRPSDVVDRRIIRAMLEIPRELFVPEDVR
                     SLAYIDREVRMSPQSNGARYLMAPRVHARLIQALELEDDDIVLEVGCGTGYGSAVMAK
                     LAQTVFGLESDPGLAATATETLSALGIDNAVVVVGSLTAGWPSEGPYDAILLSGAVPE
                     VPATLFDQLKDGGRLVAVIADGTVGRATIWQRSGQNVSPRTLFDAAAATLPGFERAPE
                     FVF"
     gene            2177..2250
                     /locus_tag="VFX46_01970"
     tRNA            2177..2250
                     /locus_tag="VFX46_01970"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2209..2211,aa:Cys,seq:gca)
BASE COUNT          430 a          795 c          714 g          445 t
ORIGIN      
        1 ttccgtgcgg aactgctcca cctcctgcaa cctgctcacg tgggtatgtt tcgcctgccg
       61 gacacgcgcc gaaacctcgc ctccctggta gatcggcact gtcagccggc cagtgacacg
      121 ggtcgtctct acatcatcga atacctcgct ctgcccgaaa cgtcggtcgt agctggcgtc
      181 cacgctcacc tcgggcagca gctcgcccca gatccggtcg acgacatggc gcgccgcctg
      241 ctcgcggtac aaagctgcga cgatgtttgg gttctcctgc tcagcaatgg ccagcgcttc
      301 ttgttgcgac ttggggagca ggtgctcagg aacactcgga tccgtcagat tgctcggcgg
      361 gtggccgatg acacgttcgt aggcagcgcg actggtgcgc aggtttgcgc gcgcgagatc
      421 cagcgccgat acggcgccgg cccttcgcgc ctgagcctgg gccacgtccg tacgtgtgac
      481 ctcgccgacg gcgaaccggt cctcggtcgc acggagctcg cgcgtcagca cgttgacgtt
      541 attctcacgc agccggacga cggcctgatc tcgaacgaca tcggcataag cgatcacagc
      601 ctcgagcaat acggattgct caatgatgtg cagattggcg cgctgcgccc gcacgacggc
      661 ctccgcctcc cgcactgtgt tcaacgtccg aaatccacga aacaatggct gggagatgtc
      721 gatgccgtag ccacgcggat tcgactcccg ggtgtcggtg cccccactgc cccgcgaaac
      781 gctcctttgg tatcccacat cggctgaacc gaagatgaat ggccggtagc cggacagtgc
      841 ccgaggcact tcctcatctg tggcccgcag gcgagcgcgt tccgcgtcca gtcgtggatt
      901 gaacttgtag gccgaggtga gcgcctgaag cagcgtctct gccgtggcgg tcccccctgg
      961 caatccggac agcacaacgg ccgccaccgc ggctgccaag cccatccgtg ccccacccat
     1021 tcgagactcg cccatgtcgc cctcaatctt agctgtcatg acggccccca tgcctcgacg
     1081 aatccaaccg cagcgcggcc gtcgaagccc tgcacccgtt cagttaaact gattcccccc
     1141 ggggccgtag cgacgcgaat ccctaggtac ctcaaaccgg aggcacaaac gaactacgaa
     1201 tcgggtgtgg ctcgcctctt gccttggcga gagtggcctg aactccacag ttatgggtat
     1261 tattgacgat cagaatacga actcgggcgc acgctcgaat ccaggcagcg tagccgcagc
     1321 agcgtcaaac agagttctgg gcgagacgtt ctggccggag cgttgccaga tcgtggccct
     1381 gcccacagtg ccatccgcaa tcacagcgac gagccggccg ccgtccttga gctggtcaaa
     1441 caacgtcgcc ggcacctcag gaacggcacc gctgaggaga atggcgtcgt atggtccctc
     1501 gcttggccag cctgctgtca ggctgcccac caccacgaca gcgttgtcga tgccgagcgc
     1561 cgatagcgtc tccgtagcgg tcgccgcgag ccccggatcc gattcgaggc cgaagacggt
     1621 ctgcgccagc ttggccatta cggcactgcc gtaccccgtg ccgcacccga cctcgagaac
     1681 gatgtcgtca tcttcgagct ctagggcctg gatcagtctt gcgtggaccc tcggggccat
     1741 caagtagcgg gcgccattcg attgtgggga cattcgaact tcgcgatcga tgtaggcgag
     1801 cgaccgcacg tcctctggga cgaatagctc gcgcggaatt tcgagcatgg cgcgaatgat
     1861 gcgccggtcc acgacatcgc tcggtcgcac ctggctttcg accatgttct tgcgttggac
     1921 cgcgaagttc accatctctc tcctcgacat atagaatgtc cgccaccggc aagccccggg
     1981 ccgaggcggc ggcaacttag cggaggcctg tcgtctctgc cagatgtctt gtcaagagcg
     2041 aaaaccggta gacgcttggg cgcaagagac cgatttcgtt gcaggactgc cggcgtcatg
     2101 ctagaggacg ctcgcacccg cgatatcttc gcggcaaacc tgagcgcgcg ccataagccg
     2161 acgctcgggg cggggcggcc acgtggcgga gtggttacgc agcggactgc aaatccgcgt
     2221 acgccggttc gattccggcc gtggcctcca atacttagct cggcgccacc cgagcattct
     2281 gcactccgtt ttgcaatatg ttcacgatct ggtgctctcc agcttggtaa tggcgccgca
     2341 atcgcgagcg ttggtgtgcg cgcttatagc gctcgaggat cgcc
//
LOCUS       DASUMG010000122         2397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_176255, whole genome shotgun sequence.
ACCESSION   DASUMG010000122 DASUMG010000000
VERSION     DASUMG010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176255"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VFX46_01975"
     CDS             <1..1050
                     /locus_tag="VFX46_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5599382.1"
                     /translation="NADTDEVLVCCHVRSETHDVRSFLFRTRSPSLFRYRPGQFITLE
                     LEIDGEPINRCYTLSSTPTRPDTISITVKRQPGGKVSNWLHDNMKPGMEIKALGPAGD
                     FSCTLRPAAKYLFLSGGSGVTPLMSMSRAHYDLGEDADIFFVHSARTPRDIIFRRELA
                     AMTNGMDRFRTAFVCEQAGDERDWGAPTGYLSPTMLKSIVPDFAQREIFCCGPEPYMA
                     AVRTMLKEADFDMAHYHEESFSFERHAPASEGAATKPAPPDAPAKGFRIEFTRLGRVI
                     ECGPDQFILDAARAAGMRLPFSCSKGMCGTCKTKKLVGNVAMNHAGGIRQREIDAGMI
                     LICCSKPLSDVVLEK"
     gene            complement(1071..2261)
                     /gene="choV"
                     /locus_tag="VFX46_01980"
     CDS             complement(1071..2261)
                     /gene="choV"
                     /locus_tag="VFX46_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015220 - choline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015871 - choline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline ABC transporter ATP-binding protein"
                     /protein_id="HEX5599383.1"
                     /translation="MSVAVDFKHVDIIFGKDTPAALKMVDAGANRAEILAKTGSVLGA
                     ADATLTVNEGEISVLMGLSGSGKSTVLRAVNGLNKVARGQVLVKDGNRMVDVASCNAE
                     TLRHIRQKQVAMVFQQFALLPWRTVRENVGFGLELAGVPEPELNERVDKQLKLVGLDA
                     WANKHVHELSGGMQQRVGLARAFATDAPILLMDEPFSALDPLIRTKLQDELLQLQKSI
                     KKTILFVSHDLEEALKIGSRITIMEGGRIVQSGQAEDIVLRPADAYVQDFIANVNPLS
                     VLTAWNVMRGVHELERAEDGWIWLDQRKTTRFKLNGQGEVEAANCDGRSTEWVCCSEV
                     EKTVPEGSTPVYWARPGTTLKTVILAMHKSGTAPVALFDEHEKFVGSIGVRNVLQAIL
                     RRGG"
     gene            complement(2258..>2397)
                     /locus_tag="VFX46_01985"
     CDS             complement(2258..>2397)
                     /locus_tag="VFX46_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline ABC transporter permease subunit"
                     /protein_id="HEX5599384.1"
                     /translation="SLGNPVNKALANRNIPLGIEAGLAIVILAIILDRVLAAKPVGVR
                     K"
BASE COUNT          424 a          772 c          752 g          449 t
ORIGIN      
        1 aacgccgaca cggacgaggt tctcgtctgc tgccacgttc gttccgagac gcacgacgtg
       61 aggagttttc tcttccgcac gcgctcgccg agcttgttcc gctaccgtcc cggtcaattc
      121 atcacgctcg agcttgagat cgacggcgag cccataaatc ggtgctacac cttgtcctcg
      181 accccgacgc ggccggacac gatctcgatc acggtgaagc gccagcccgg cggcaaggta
      241 tcgaactggc ttcacgacaa catgaagcct gggatggaga tcaaggcgct cgggcccgcg
      301 ggagacttca gctgcacgct gcggccggcg gcgaagtatc tctttctgtc cggcggctca
      361 ggcgtgacac cgctcatgtc gatgtcgcgc gctcactatg acctcggcga ggacgccgac
      421 atctttttcg tgcacagcgc gcgcacgccg cgcgacatca tcttccggcg tgagctcgcc
      481 gcgatgacga acggcatgga ccgctttcgc acggcgttcg tctgcgagca ggcgggtgac
      541 gagcgcgact ggggcgctcc gaccggttac ctgtcgccca ctatgctaaa gtcgattgtc
      601 cccgacttcg cgcaacgcga gatcttctgc tgtggccccg agccctatat ggcggccgtg
      661 cgcaccatgt tgaaggaggc tgacttcgac atggcccact atcacgagga gagcttctcg
      721 ttcgagcggc acgctccggc ttccgaaggc gccgcgacca agccggcgcc gcccgacgca
      781 ccggctaaag ggttcagaat cgagttcacg cggctcggac gcgtcatcga atgcggaccc
      841 gaccaattca tcctcgacgc ggcgagggcg gcggggatgc gccttccctt ctcgtgcagc
      901 aagggcatgt gcggcacctg caagacgaag aaactcgtcg gcaatgtcgc catgaaccac
      961 gccgggggca tcagacagcg ggagatcgat gccggcatga tcctgatttg ctgtagcaag
     1021 ccgctgagcg acgtcgtgct ggagaagtag acctgccccg ccgcggacgt tcagccgccg
     1081 cggcgcagta tggcttgcag cacgtttcgc acgccgatcg agccgacgaa cttttcgtgc
     1141 tcatcgaaaa gcgcgaccgg agcggtcccc gatttgtgca tggcgaggat cacggtcttg
     1201 agcgtcgtgc cgggccgcgc ccaatagacg ggcgttgaac cctccggcac ggttttttcg
     1261 acctcgctgc agcacaccca ctcggtgctc cgaccgtcgc aatttgcagc ctcgacctcg
     1321 ccttggccat tgagcttgaa gcgcgtggtc ttgcgctgat cgagccatat ccagccgtcc
     1381 tccgcgcgtt cgagctcgtg cacgccgcgc atcacgttcc acgccgtcaa caccgagagc
     1441 gggttcacgt tggcgatgaa gtcctggacg taggcatcgg ccggccgcag cacgatatcc
     1501 tccgcctgtc ccgattgcac gatgcggccg ccctccatga tggtgatgcg gctgccgatc
     1561 ttgagcgctt cctcgaggtc atggctgaca aagaggatcg tcttcttgat cgacttctgc
     1621 aattgcagga gttcgtcttg cagcttggtg cggatcaggg gatcgagcgc cgagaacggc
     1681 tcgtccatca acaggatcgg tgcgtcggtg gcaaacgcgc gggcgaggcc gacgcgctgc
     1741 tgcataccgc ccgagagctc atgcacgtgc ttgttggccc aggcgtcgag gccgacgagc
     1801 ttcaattgct tgtcgacgcg ctcgttgagc tcgggttccg gaacgccggc aagctcgagc
     1861 ccgaagccga cattttcgcg caccgtccgc cagggcagaa gcgcgaactg ctgaaagacc
     1921 atggcgacct gcttctggcg gatgtgacgc agcgtttcgg cattgcacga ggccacatcg
     1981 accatgcggt tgccatcctt cacgagcacc tgcccgcgcg ccactttgtt gagcccgttc
     2041 acggccctga gcacggtcga tttgcccgag cccgacagcc ccatgagcac cgagatctcg
     2101 ccctcattga cggtgagggt ggcatccgcg gcgccgagca cggaaccggt cttggccagg
     2161 atctcggcgc ggttggcgcc ggcatcgacc atcttgagag cggccggagt gtctttgccg
     2221 aagatgatgt caacatgctt gaaatcgacg gcgacgctca tttccttacc ccaacaggtt
     2281 ttgccgcgag cacgcggtcg aggatgatag cgaggatgac gatcgcaagg cccgcctcga
     2341 taccgagggg aatgttgcga ttggcgaggg ccttgttcac ggggttgccg aggctcg
//
LOCUS       DASUMG010000123         4127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_979777, whole genome shotgun sequence.
ACCESSION   DASUMG010000123 DASUMG010000000
VERSION     DASUMG010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979777"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2946)
                     /locus_tag="VFX46_01990"
     CDS             complement(<1..2946)
                     /locus_tag="VFX46_01990"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX5599385.1"
                     /translation="MSRQALNEAFARTSFLHGTNAAYIEEMQALYEQNPGSVSDEWRL
                     FFQSLQEERGAVEAEVHGPSWARPLEELTQNGQSELVAALAGDYVGAERRIRDKLKER
                     AYAAGFDLSMAALLRATQDSIRALMLIRAYRVMGHLIADLDPLGLSERSVHRELKPET
                     YGFTEADLDRPIFIDKVLGLETATIRQILKILRRTYCGQIGVEFMHITSPAQKAWIQE
                     RIEGEGKGIKFTPEGRRAILNKLIEAETFEKFCDVKYTGTKRFGLEGGESMIPALEQI
                     IKRGGQLGVKEIVIGMAHRGRLNVLANVMGKPYRAIFNEFKGGSFKPDDVEGSGDVKY
                     HLGASSDRVFDGISVHLSLTANPSHLESVDPVVLGKVRAKQDQYGCPPDDRTVVMPLL
                     IHGDAAFAGQGIVAECFGLSGLKGHRTGGSVHFIINNQIGFTTNPRYSRSSPYCTDVA
                     KMVEAPIFHVNGDNPEAVVHVAKIATEFRQRFQKPVVIDMICYRRHGHNEADEPAFTQ
                     PVMYKAIKSHPTTLELYSKRLIEEGVVTAEEVEEARARFRAKLEEEYKAAETYRPNKA
                     DWLDGRWAGLEPNEKGDWRGNTGVDLDILREVGRRITTIPADFNAHRTVQRLLERRRE
                     MIETGEGIDWAMAEHLAFGTLLKEGFPVRLSGQDVERGTFSQRHAVLFDQDTGRRYTP
                     LKHVSPDQAKFEVINSMLSELAVLGFEYGYSLAEPHALVIWEAQFGDFANGAQVIIDQ
                     FISSGERKWLRMSGLVMLLPHGYEGQGPEHSSARLERYLQLSAEDNWQVANCTTPANY
                     FHILRRQLHRNFRKPLVLMTPKSLLRHKRVVSHLDEFGPGTSFHRVLWDGADRSHEHF
                     DASTVELKPDNEIRRVVLCTGKVYYDLYELRDQLGIDDVYLMRIEQLYPFPARALMAE
                     LGRFVNAEFVWCQEEPRNMGAWTYIEPNLEWVLQHIRAKHLRPRYVGRAAAAATATGQ
                     LSRHLQEQQT"
     gene            complement(3109..4008)
                     /gene="sucD"
                     /locus_tag="VFX46_01995"
     CDS             complement(3109..4008)
                     /gene="sucD"
                     /locus_tag="VFX46_01995"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEX5599386.1"
                     /translation="MAILVDKNTKVICQGITGSQGTFHTKQAKAYGTQIVAGVTPGKG
                     GAKHPDPELADVPLFNTVAEAKAKTGATATSIYVPPAFAADAILEAIDAEIPLIVCIT
                     EGIPVMDMVKVKRALQGSSSRLIGPNCPGVLTPDECKIGIMPGNIFKRGHVGIVSRSG
                     TLTYEAVKQTSDAGLGQSTAVGIGGDPVKGTEFIDVLEMFLADPDTHSIIMIGEIGGS
                     AEEEAAEFIKSEAAKGRKKPMVGFIAGVTAPPGRRMGHAGAIISGGKGKAEDKLEAMR
                     SAGIAIADSPAALGTTLVKVLNG"
BASE COUNT          709 a         1317 c         1263 g          838 t
ORIGIN      
        1 tgtttgctgc tcctgcagat gccgcgaaag ctgccccgtt gccgtcgcgg cggcagcagc
       61 ccggccgacg taccgcggcc gcagatgctt cgcccgaatg tgttgcagca cccattccag
      121 gttcggctcg atgtaggtcc aagcgcccat gttccggggc tcctcctggc accagacgaa
      181 ctctgcgttc acaaaacgcc cgagctcggc catgagtgcg cgcgccggga atggatagag
      241 ctgctcgatg cgcatcaggt agacgtcgtc gatgccgagc tgatcccgca gctcgtaaag
      301 gtcgtagtag acctttccgg tgcacagcac gacccgccgt atctcgttat ccggcttgag
      361 ctccaccgta gacgcgtcga agtgctcgtg gctccggtcg gcgccgtccc aaagcacgcg
      421 atggaagctc gtgcctggcc cgaactcgtc gaggtgcgaa acaacccgct tatgcctcag
      481 cagcgatttc ggcgtcatca gcaccagcgg cttgcggaag ttgcgatgga gctgccggcg
      541 caagatgtgg aagtagttcg cgggcgtggt acagttcgcg acctgccagt tgtcctcagc
      601 cgaaagctgc aggtaacgct ccaggcgggc ggacgagtgt tccggcccct ggccctcgta
      661 gccgtgaggc agcagcatca caaggcccga catcctgagc catttgcgtt cgccggacga
      721 gatgaactgg tcgatgatga cctgggcacc gttggcgaaa tcgccgaatt gcgcctccca
      781 aatgaccagc gcatgcggct cggcgaggct gtaaccgtac tcgaagccga ggacggccag
      841 ctctgacagc atcgagttga tgacctcgaa cttcgcctgg tcaggcgaaa cgtgcttcag
      901 cggcgtgtac cggcggccag tgtcctgatc gaagagcacg gcgtgacgtt gcgagaacgt
      961 gccgcgctcc acgtcctggc ccgagagccg caccgggaag ccttccttaa gcagcgtgcc
     1021 gaaggccagg tgctcggcca tggcccagtc gataccctcg cccgtctcga tcatctcgcg
     1081 gcgccgctca agcagccgct ggacggtcct gtgggcgttg aaatcggcgg ggatggtcgt
     1141 gatccggcgc cccacctccc gcaggatgtc gagatcgacg ccggtgttgc cgcgccaatc
     1201 gcctttttca ttcggctcca aacccgccca gcggccgtcc aaccagtcgg ccttgttcgg
     1261 acgataggtt tcggcggcct tgtattcctc ttcgagcttg gcgcggaatc gagcgcgtgc
     1321 ctcctcgacc tcctccgccg taaccacgcc ttcctcgatc agccgcttcg agtagagctc
     1381 gagcgtggtg ggatgcgatt tgatggcctt gtacatcacc ggctgggtga aggccggctc
     1441 gtccgcctcg ttatgcccgt gccgccgata gcagatcatg tcgatcacga ccggcttctg
     1501 gaaccgctgg cggaactccg tcgcgatctt ggccacgtgg accacggctt ccgggttgtc
     1561 gccgttgacg tggaaaattg gcgcctcgac catcttcgcc acgtctgtgc agtacggcga
     1621 cgaccgcgag taacgggggt tggtcgtgaa cccgatctgg ttattgatga tgaagtgaac
     1681 ggagccgccc gtgcggtgcc ccttgaggcc cgacaggcca aagcattccg ccacgatgcc
     1741 ttggccggcg aacgccgcgt cgccatgaat gagaagaggc atcacgacgg tgcggtcatc
     1801 tggcgggcag ccgtattggt cctgctttgc cctgaccttg cccagcacca cgggatcgac
     1861 gctctccagg tgcgacgggt ttgccgtcag cgacaggtgc actgagattc catcgaagac
     1921 gcgatctgac gatgcgccga ggtggtactt cacgtccccc gagccctcga catcatcggg
     1981 cttgaaggag ccgcctttga actcattgaa gatcgcccgg taaggcttac ccatcacgtt
     2041 ggccagcacg ttgagccggc cacgatgggc catgccgatc acgatctcct tcacgccgag
     2101 ctgaccaccg cgcttgatga tctgctcgag cgccgggatc atcgactcgc cgccctcaag
     2161 tccgaagcgc ttcgtgcccg tgtacttgac gtcgcagaac ttctcgaagg tctcggcttc
     2221 gatcagctta ttcaggatcg ctcggcggcc ttcaggcgtg aacttgattc ccttgccctc
     2281 gccttcgatc cgctcctgga tccaggcctt ctgagccggc gaggtgatgt gcatgaactc
     2341 gacgccgatt tggccgcaat aggtccgccg tagaatcttc aggatctgac ggatggtcgc
     2401 cgtctcgaga ccgagaacct tgtcgatgaa gatcggccgg tcgagatctg cttccgtgaa
     2461 gccgtaggtc tccggcttca gctcgcgatg gacgctccgt tcgctgaggc ccagcggatc
     2521 gagatccgca atcagatggc ccatcacgcg ataggcccgg atcagcatta gggcgcggat
     2581 agagtcctga gtggcccgca gcaacgcggc catggaaagg tcgaaacccg ccgcgtacgc
     2641 tcgctccttc agcttgtcgc ggatccgccg ctccgcaccg acgtaatctc ccgccaaggc
     2701 tgcgacgagc tccgattgcc cgttttgggt gagctcctca agcggccgcg cccaagacgg
     2761 accgtgcacc tcggcttcca ccgcccctcg ctcttcctgc aagctttgga agaacagccg
     2821 ccactcgtcg ctaacagagc cggggttctg ctcgtagagc gcctgcatct cctcgatgta
     2881 ggcagcattg gttccgtgca ggaaggaagt tcgcgcaaag gcctcgttca gagcttgtcg
     2941 tgacatcgtt tcactttgtc cggattaagc gttcgcaggc ctgcgaacgc tgcgacgcac
     3001 ccgatgggca cctgttgaca caccgcctga tttagtgcag gcgcgccgtt cctttaaggc
     3061 ccgccgagcg aggtcccagg ttgtactcct taccacgcga cctgttacct agccgttgag
     3121 gactttgacc aacgtcgtgc caagtgcggc ggggctgtcg gcaatggcga ttccggccga
     3181 ccgcattgct tcgagcttgt cttcggcctt gccctttccc cccgagatga tcgcccccgc
     3241 atgacccatg cgccggcccg gcggagctgt cacgccggcg atgaacccga ccatcggctt
     3301 cttccggccc ttggcggctt cgctcttgat gaactcggcg gcttcttcct cggccgagcc
     3361 gccgatctct ccgatcatga tgatggagtg cgtgtccgga tcggcgagga acatctcgag
     3421 cacatcgatg aactcggtgc ctttcacggg atcgccgccg ataccgacgg ccgttgattg
     3481 accgaggccg gcatccgagg tctgcttgac ggcctcatag gtgagcgtgc ccgagcgcga
     3541 tacgatgcca acgtgcccgc gcttgaagat gttgccgggc atgatgccga tcttgcattc
     3601 gtcgggagtg agcacgccag ggcagttggg gccgatcagc cgggacgagg atccttgcag
     3661 tgcccgcttc accttcacca tgtccatcac gggaatgccc tccgtaatgc acacgatgag
     3721 gggaatttcg gcgtcgattg cttcgaggat ggcatcagcc gcaaacgccg gcggcacgta
     3781 gatggatgtt gcggttgcac ccgtctttgc cttggcttcg gctaccgtat tgaaaagggg
     3841 tacgtccgcc agctccggat cggggtgctt ggcgccgcct ttcccagggg tgacacccgc
     3901 gacgatctgc gttccgtaag ccttggcctg cttggtgtgg aaggtgccct ggctaccagt
     3961 gatgccctga cagatgactt tggtgttctt gtcgacgagg atggccatta ctgcgctagt
     4021 cctgccctct tgcctcgaat ggtgcttgct tgtgggacag attgagcacc gaatcaagtc
     4081 ggtcgatggg cgaaagtgtc tgctcgatct gcagaattcc accggcg
//
LOCUS       DASUMG010000124         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_980195, whole genome shotgun sequence.
ACCESSION   DASUMG010000124 DASUMG010000000
VERSION     DASUMG010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980195"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFX46_02000"
     CDS             complement(<1..447)
                     /locus_tag="VFX46_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502422.1"
                     /note="subunit A of antiporter complex involved in
                     resistance to high concentrations of Na+, K+, Li+ and/or
                     alkali; in S. meliloti it is known to be involved with K+;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
                     /protein_id="HEX5599387.1"
                     /translation="MSTDAILLTVLVVMPFVGSVVAALLPTNAHDLEAWWAAGIAFIG
                     LAIASTFSQPMAEGAIIRAEFEWVPQLGLNFILRLDGFAWSFVVLVCLIGLLVILYAR
                     YYIRPEDPASRFFSYLLGFKGAMLGIVLSGNLLQLVFFWELTSLYSF"
     gene            786..1166
                     /locus_tag="VFX46_02005"
     CDS             786..1166
                     /locus_tag="VFX46_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599388.1"
                     /translation="MFAGPDRDEDEIHWVIVRRVEFDWVGKPEEDSCGPRQVIEPCVR
                     HCHSVFDRGGAEGLAVRQGFQDDLAWDAGAPPKVFRSGGENGGLRIDRLVQKQVLIAE
                     NRRELYRFHHTPPGESGITAPERA"
     gene            1789..>2091
                     /locus_tag="VFX46_02010"
     CDS             1789..>2091
                     /locus_tag="VFX46_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5599389.1"
                     /translation="MSETFGFIGLGNMGHPMAGRLLDAGYSLLVHDVRTSVVDQFVER
                     GAKRASSPAEVASQVETVFLSLPTPPVVETVALGENGLTSGDKVKRVIDLSTTGPQT"
BASE COUNT          440 a          621 c          641 g          389 t
ORIGIN      
        1 gaacgagtat aggctcgtga gctcccaaaa aaagacgagc tgcagcaggt tgcccgacaa
       61 cacgatgccg agcatggcgc ccttgaagcc cagcaaatac gagaagaagc gcgaggccgg
      121 atcctctggc ctgatgtaat agcgggcata gaggataacg agcagcccga tcaggcacac
      181 aaggacaacg aagctccagg cgaagccgtc gagccgcagg atgaaattga gccccaattg
      241 gggcacccac tcgaactcgg cccggataat cgccccttcc gccattggct gggagaaggt
      301 gcttgcaatg gcaaggccga tgaacgctat ccccgctgcc caccacgctt ccaggtcatg
      361 tgcattggta ggcaggagcg ctgcgacgac gctgccgaca aacggcatta cgaccaggac
      421 agtcagaagg atggcgtcag tcgacatgct cggcaagcaa cctttcgttt ttgggatggc
      481 gggtcgcgcc gactttaggg tctgtctgga aacagcaaca agacggcgcc aagccgcggc
      541 gcgacaaggc ttaagggcaa ttgcgccccg cgcgccacat gcaattcctt atttacggga
      601 aggttgcggc taccgcaaac gtcgccgcgc aactatcaag aggagttctt ggcgtctgag
      661 tctgcgctcc ggctgagagc cgatttgatc tcctgcggag ttgaagcagt ccgcagtgcc
      721 tgtcgcaccg aaggttctcg taggttacgg cacaaatgcc caagtgctga cagtagccca
      781 cttctatgtt cgctgggcca gaccgtgacg aagacgagat ccactgggtt atcgtccggc
      841 gcgtcgaatt cgattgggtg ggcaagcctg aagaagacag ctgcgggccg cggcaggtta
      901 tcgagccctg cgtgcggcac tgccactccg tgtttgatcg cggtggagcc gagggcctcg
      961 cggtccgaca gggctttcag gatgacctcg cttgggacgc cggtgctccg cccaaggtgt
     1021 tccgcagcgg cggcgagaac ggcggccttc gaatcgatag gctggtccag aagcaggtcc
     1081 tcatcgccga gaatcgtcgt gagctctaca ggttccacca tacgcctcct ggcgagagcg
     1141 gcatcacggc cccagagcgg gcctagggca gccgagactg atggcattcc atgtcgcagg
     1201 gcatttgctg gcacgttcta ttggcatggc gaccaatcag aacccaacgc gcgtaccagc
     1261 catcctgagg ctaatcgatt gaccgacaac cctttcctgg tagatcacga cgtttcgctc
     1321 cctccaggat gatcttgggt ggagcaccct ttagtgcgga gacgtgcccg ccgcaagagg
     1381 cgatcatctc ctcagtcttg agaagtgccc tgtcggcatc acaatcgcgg gaggggatta
     1441 gccgaatcct gagcagatcg atatgcgtat ttcgtcgcaa tccgtgcggt gagcgtgaga
     1501 cccgttgcac aagccatctt ggctccgaag tcaccgccgt cgaaatgcga gccagcagga
     1561 cccggcgaag actgcaaatc ggaatagctg cgccgccaat ctgtcgattg tattaccgta
     1621 acagcatctg tattctggcc tcgtacgaga cagcccccac ggcatggtag ccggccagtg
     1681 gcgcctggtt gctggccgcg cgggatcagc aggtttggcg ccggttcatt gtcagcttcg
     1741 ccctctgccc agagcgcaaa gtccggcgca cgcaatcggg aggacgcatt gagcgagacc
     1801 tttggattca tcggcctcgg caacatgggg caccctatgg ccgggcgttt gcttgacgcc
     1861 ggttattctc tgctagttca cgacgtgcgc acgagcgtcg tcgatcagtt cgttgaacga
     1921 ggcgcgaaac gggcctcgtc accggccgaa gtcgcctccc aagtggaaac cgttttcctg
     1981 tcgctgccta cgccaccggt cgtggagacg gtggcccttg gggagaacgg cctgacatcc
     2041 ggagataagg tcaagcgggt catagatctt tccaccacag gcccgcaaac c
//
LOCUS       DASUMG010000125         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_176826, whole genome shotgun sequence.
ACCESSION   DASUMG010000125 DASUMG010000000
VERSION     DASUMG010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176826"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /locus_tag="VFX46_02015"
     CDS             <1..1366
                     /locus_tag="VFX46_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX5599390.1"
                     /translation="AMRDVEAVLHPATNLALHRTTGPLVLERGEGVFVWDKEGKRYIE
                     GLAGLWCTGLGYGNQELIEAAREQMSRLSFTHLFGGRSHEPAIALAEKLKELVPAPTS
                     KVFFTCSGSEANDTQIKLVWYYNNARGLTKKKKIISRMKAYHGVTIAAGSLTGLPVFH
                     ADFDLPMQRVLHTDCPHYWRYAEEGESEEEYATRLANNLEDLIQREGPETIAAFIAEP
                     VMGAGGVIPPPRTYFQKIQEVLRRYDIMMIADEVICGFGRTGNWFGSQTFGIDPDTIT
                     MAKAITSAYMPLGAVTVSEPVYEALLEESKKLGVFAHGFTYTGHPVSCAVALKTLEIY
                     ERIDIVRHVQSVAPVFETRLRALRDHPLVGETRGVGLIGGIELVKNKSTKESFDVRQG
                     VGPKVVKLAEEEGLICRAVAGDTIALAPPLIINGAEINLMFDALGRALDRAEEWVRKE
                     GLRG"
     gene            1461..2474
                     /locus_tag="VFX46_02020"
     CDS             1461..2474
                     /locus_tag="VFX46_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599391.1"
                     /translation="MAMSVRKARHAGVAAAIAVACVMALAADIGFAQSGLQLDYGIAD
                     GLRLRVVILNAASPPAAPPTAIEEPETEPERELIWRHTFGAERRTPAVHSKSAARQPL
                     RLNADVVVLHGVSNVNSVRQMLPARDYHLLVSRQVLRRGESQVATTAIAVRRDSGLRV
                     IAQDHLLQLAEVSENSSTPLAAGTAARLHASSGSLWVLALDLSQCSAEMPEDDACEAA
                     SRQLAAIEDWATQRMKEGEPVIIGGRFHTALTQTSLPSQLRKLSRFPGFEPTGRDCAT
                     DDGQIDSAYVLASPGFRFDQIGLNGRFEPVNEKAPEKGCLMIVEADLRSSWAQTIIRA
                     AHH"
     gene            complement(2452..3210)
                     /locus_tag="VFX46_02025"
     CDS             complement(2452..3210)
                     /locus_tag="VFX46_02025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599392.1"
                     /translation="MPVTSNAERKTLILTGASRGIGHATVKRFASAGWRVITCSRQPF
                     PENCPWEMGPEDHIQVDLSDPEDTRRAIAEMRRRLEAQGSRLNALVNNAGISPKGPGG
                     SRLGTIETSLEDWQTVFQVNFFAPIMLARGLIEELERAKGAVVNVTSIAGSRVHPFAG
                     AAYATSKAALAALTREMAHDFGPRGIRVNAISPGEIDTSILSPGTDKIVREQIPLRRL
                     GEPEEVAKAIYFLCTEQSSYVNGAELHINGGQHV"
     gene            complement(3328..>3554)
                     /locus_tag="VFX46_02030"
     CDS             complement(3328..>3554)
                     /locus_tag="VFX46_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate oxidase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5599393.1"
                     /translation="SRPLESRFALEKAVAFYTAKYAVGEIPRPPYWSGFRVVPLEMEF
                     WHDRPFRLHDRVQFRRTGPAEPWVKTRLYP"
BASE COUNT          660 a         1107 c         1135 g          652 t
ORIGIN      
        1 tgccatgcga gatgttgagg ctgtcttgca ccctgcaacg aatctcgcgt tgcatcgaac
       61 aaccggtccc ctggtattgg agcggggcga aggagtcttc gtctgggaca aggaaggcaa
      121 gcgttatatc gagggccttg ctggcctttg gtgcacgggc ctcggctacg gtaaccaaga
      181 gctgatcgaa gcggcgcgcg agcagatgag ccgtttgtcg ttcactcatc tgttcggcgg
      241 gcgcagtcac gagccggcga tcgcactggc agagaagctc aaggagctgg tgcccgcgcc
      301 gacgtcgaag gtcttcttca cctgctcggg ctcggaggcg aacgacactc agatcaagct
      361 cgtctggtac tacaacaatg cccgtgggct gacgaagaag aagaagatca tcagtcgaat
      421 gaaggcgtac cacggcgtga cgatcgcggc ggggagcctc actggtctgc cggtcttcca
      481 cgccgatttc gacctgccga tgcagcgggt attgcacacg gactgtccgc attactggcg
      541 gtatgcggag gaaggcgaga gcgaggaaga gtatgccacg cgccttgcca ataatctcga
      601 ggatctgatt cagcgcgaag gcccggagac catcgccgct ttcatcgccg agcccgtcat
      661 gggagctggc ggggtcattc ctccgccgcg gacatacttt cagaagatcc aagaggtgct
      721 gcggcgctac gacatcatga tgatcgccga cgaggtcatc tgcggcttcg ggcgcacggg
      781 caactggttc ggctcacaga ctttcgggat tgatcctgac accatcacga tggccaaggc
      841 gatcacgtcc gcctacatgc ccctcggcgc cgtaacggtc agcgagcccg tgtatgaggc
      901 tctgctcgag gagagcaaga agctcggagt cttcgcccat gggttcacct acacaggtca
      961 ccccgtttcc tgcgctgtcg ccctgaaaac cctcgagatc tacgagcgca tcgacatcgt
     1021 ccgacatgtg cagagcgttg cgccggtgtt cgagacgcgc ctcagggcgc tcagggatca
     1081 cccgctcgtg ggtgagacgc ggggcgtcgg cctcatcggc ggcatcgagc tcgtgaagaa
     1141 caagtcgacc aaggaaagct tcgacgtgcg gcagggcgtg gggccgaagg tcgtgaagct
     1201 ggcggaggaa gaggggctga tttgccgtgc tgtcgccggc gatacgatcg cgctcgcacc
     1261 gcccctgatc atcaacggtg ccgaaatcaa tctgatgttc gacgctcttg ggcgggcgct
     1321 cgatcgcgcc gaggagtggg tccggaagga gggcctgcga ggctgatagc tatcgttagt
     1381 ggctccgcgg tctcttctga ttgggtttcc ggaacggcgc gagtcaggca cactcgtcgc
     1441 aacctgagcg ggcagcgggg atggcgatga gtgtgcggaa ggcgaggcac gccggcgttg
     1501 cagcggctat cgccgttgca tgcgttatgg cgctcgccgc tgacatcggt ttcgcccaat
     1561 caggtctgca gctcgactat ggcatagccg acggcctgcg gctgcgcgtg gtcatcctga
     1621 acgcggcttc tccgcccgca gctcctccga ccgcgatcga agaaccggag acggagcccg
     1681 agcgtgagct gatctggcgg catacgttcg gagcggagcg ccgaacccca gcggtgcaca
     1741 gcaaatcagc ggctcggcag ccgctccgtc tcaatgcgga tgtcgtcgtg ctccacggcg
     1801 taagcaacgt gaattcggtg cgccagatgc ttccggcgcg cgactatcac ctgctggtct
     1861 cacgacaagt tttgcggcgc ggtgagtctc aggttgcgac aacagccatt gccgtccgtc
     1921 gggactctgg cctccgcgtc atcgcccagg atcatctgct gcaattggca gaagtttcgg
     1981 agaattcgag taccccactt gcagccggca ccgcagcgcg actccacgca tccagcgggt
     2041 ctctctgggt gttggcgctc gacctttcgc agtgctccgc cgaaatgccg gaggacgatg
     2101 cctgcgaagc cgcaagccgg cagctcgccg ccattgagga ttgggcgacg caacggatga
     2161 aggaaggcga gcctgtgatc atcggaggca ggtttcacac cgctctgacc caaacgagct
     2221 tgccaagcca gctccgcaag ctctcacgct ttccgggctt cgagccgacc ggtcgcgatt
     2281 gtgcgaccga cgatggtcaa atagactcgg cgtacgttct cgcttcgcct ggcttccgct
     2341 tcgatcagat cgggctcaac ggccgcttcg agccggtgaa tgaaaaagcc cccgaaaagg
     2401 gctgcctgat gatcgtcgag gccgaccttc gctcttcctg ggcccaaacc atcatacgtg
     2461 ctgcccacca ttgatgtgca gctcggcccc gttcacgtag ctcgactgct ccgtgcacag
     2521 gaagtagatg gccttggcga cctcctccgg ctcgccgagg cgcctgagcg gaatctgctc
     2581 acggacgatc ttgtcggtgc cgggcgagag aatggacgtg tcgatctcgc caggggagat
     2641 ggcgttcacg cgaatgcccc gcggaccgaa atcatgagcc atttcgcgcg tcagcgccgc
     2701 cagcgccgcc ttggacgtcg cgtaagccgc gccggcaaac gggtgcacgc ggctgccggc
     2761 gatcgaggtc acattcacga cggcgccctt tgcccgctcc agctcttcga tcaacccgcg
     2821 ggccagcatg atcggggcaa agaaattcac ctgaaatacc gtctgccagt cctccagcga
     2881 ggtttcgatg gtgccgaggc ggctgcctcc cggccccttg gggctgatgc cagcattgtt
     2941 gacgagggcg ttgagccgcg acccttgcgc ctccagccgc cggcgcattt cggcaatggc
     3001 ccggcgcgta tcctcaggat cggaaagatc gacctggata tggtcctccg gccccatctc
     3061 ccaggggcag ttctccggga acggctgccg cgagcaggtg atcacgcgcc acccagcgga
     3121 ggcgaatcgc ttgacggtcg catgcccgat cccgcgcgag gcgccggtca ggatcagggt
     3181 ctttcgctcg gcattgctgg ttacgggcat ggtgtcacag catccatgaa caacgaccac
     3241 gagcttcgga tgtgcgttgc agcatagccg aaatcaatgc ggcaaggcac aagtttaggg
     3301 ccggcgcctc ccctcgcccg gttgcgctca gggataaagg cgcgtcttca cccacggctc
     3361 tgccggtcct gtgcgccgaa actgcacccg atcatgcagc cggaatggcc ggtcgtgcca
     3421 gaactccatt tccagtggca ccacgcgaaa ccctgaccaa tagggcgggc gtggaatttc
     3481 cccgaccgcg tacttggccg tatagaacgc aacggccttc tccagcgcaa agcggctttc
     3541 gagcggccgg gatt
//
LOCUS       DASUMG010000126         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_257183, whole genome shotgun sequence.
ACCESSION   DASUMG010000126 DASUMG010000000
VERSION     DASUMG010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257183"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFX46_02035"
     CDS             <1..669
                     /locus_tag="VFX46_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5599394.1"
                     /translation="DSSPMYASSQPTIGYGLRKLGYPQVFAAEKVWTSRPANGPAQIE
                     RTRAYWGIPKLDLVQVHNLVAWEEHLETLFAMKAEGKIRYVGITTSEGRRHREFERIM
                     ATQPIDFIQVTYNVLDREVESRILPLAQERGIAVIINRPFRQKELIWHVERHPLPSWL
                     AETGASTWPQLLLKFIISHPAVTCVIPATTRVDHVLENMAAGSGLFPDEAVRQRISDY
                     VRQL"
     gene            671..1351
                     /locus_tag="VFX46_02040"
     CDS             671..1351
                     /locus_tag="VFX46_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HEX5599395.1"
                     /translation="MSEWWTYSLSDMLLFSPRVYYRLFELHNHALWPAQLATVAAGLG
                     MVFALARPSTLLTRITFVVLGILWIWIAWAFFGERYATINWAAPYVAPAYGLEGLMLI
                     ATGLLRNRFSFGSAAAFTRTAAIILLAAILIGYPLIAPAVGRSWSAAEVFGIAPDPTA
                     LATLAVLAASPRGGRLVLMVIPLLWIFISTATLWTLQSPEYWIAPLAAVLALAITCLR
                     MEPRRSAA"
     gene            1481..2254
                     /locus_tag="VFX46_02045"
     CDS             1481..2254
                     /locus_tag="VFX46_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599396.1"
                     /translation="MILECRDLERSFGGLKALKGVTFGIEKGEIFGLVGPNGSGKTTI
                     VNAITGFYPPDRGQIIFEGKDITGIRPHQVAPLGIARTFQNLALFRGMSVLDNIMMGR
                     HTHMRPSALASLFYWVWAEPEEIRQRELVEDTIDFLQLQDVRDLPIEVVPLGLQKRIE
                     LARALVSEPRLLILDEPMAGMNQEEKEYMARFILDAREERGLTVLLIEHHMDVVSALC
                     DRMTVLSYGEQIASGTPAEALSNPAVIEAYLGTKRRHAA"
     gene            2244..>2691
                     /locus_tag="VFX46_02050"
     CDS             2244..>2691
                     /locus_tag="VFX46_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5599397.1"
                     /translation="MRPEGSTVKAVDVGLDLATDDPGAVPRENVGLDSGTLISLLAKN
                     AQTHGSSVAMRERDRGIWREFTWSDVLSEVLAFAAALDAHGFQPGDGLLVIGDNRPRL
                     YFGMLSAAALRGYPAPVYPDVPPDDLEYYAGNSRARFALAEDQEQVD"
BASE COUNT          477 a          852 c          831 g          531 t
ORIGIN      
        1 gattcctcgc ccatgtacgc atcctcccag ccaaccatcg gctatggcct gagaaagctc
       61 gggtacccgc aggtctttgc cgcggagaag gtgtggacat cgcggcccgc caacggccca
      121 gcgcaaattg agcggacgcg ggcctactgg ggcattccca agctcgatct ggtgcaagtg
      181 cacaatctgg tcgcctggga ggagcaccta gaaaccctgt tcgccatgaa ggccgagggg
      241 aagatccgct atgtcggcat caccacgtcg gaagggcgtc gtcaccgtga gttcgagcgg
      301 atcatggcga cccagccgat cgacttcatc caggtgacct acaatgtgct cgaccgggag
      361 gtcgaatctc gcatcctgcc gctcgcccag gagcgcggca tcgccgtcat catcaaccgg
      421 ccgttccggc agaaggagct catctggcac gtggagcgcc atcccttgcc gtcatggctg
      481 gccgagacag gggcatccac ctggccgcag cttctgctga aattcatcat ctcgcatccg
      541 gccgtcactt gcgtcatccc ggcgacgacg cgcgtcgacc acgtgctgga gaacatggcg
      601 gcaggctcag gccttttccc cgacgaggcc gtgcgccagc ggatatcgga ttacgtgagg
      661 cagctctgac atgtcggaat ggtggaccta ctccctttcc gacatgctcc tattctcgcc
      721 ccgcgtctat taccgcctat tcgagttgca caaccatgct ctgtggccgg cgcaactcgc
      781 gacggtcgcc gcaggtctgg ggatggtctt cgcattggcg aggccgagca ccctgctgac
      841 ccgcatcacg ttcgtggtgc tgggtatact gtggatctgg atcgcctggg cgttctttgg
      901 ggagcgctac gcgacgatca actgggcggc tccctacgtc gctccggctt atgggctgga
      961 aggcctgatg ctaatcgcga ccgggctgct aaggaatcgg ttcagcttcg gctctgccgc
     1021 tgctttcact cgcacggccg cgattatcct gctcgcagcc atcctcatcg gttatccgct
     1081 cattgcaccc gcggtcggac gatcatggtc cgccgcagag gtctttggca ttgcgcctga
     1141 tccaacggcg ctcgcgacgc tggcggtcct tgcggcatcg ccaaggggcg gacgactagt
     1201 gctgatggtc ataccgctgc tttggatctt catcagcacg gcgacgcttt ggacgctgca
     1261 gtcgcccgaa tactggatcg ctcctctggc tgcggttctt gcactggcca tcacgtgcct
     1321 gcggatggaa ccgcgacgtt ccgcagcatg agaaacctgc accagggctg ctattgctcg
     1381 cctagagccg cgcgcataaa gtacgcccgg tagcgtgcgg atatacaagg aaaaacaatt
     1441 ctgggcacgg ggaggtaact tccatgcgag ccggcgccaa atgattctcg agtgtcgcga
     1501 tcttgagcgg tccttcggcg gcctcaaggc gttgaagggg gtcacgttcg gcatcgaaaa
     1561 gggcgaaatt ttcggtctcg ttggcccgaa cggctccggc aagacgacca ttgtcaacgc
     1621 tatcactggc ttttatcccc ctgaccgcgg ccaaatcatc ttcgaaggaa aggacattac
     1681 gggcatccgg ccccatcagg tggcgccgct cggcatcgcg cgtacctttc agaacctcgc
     1741 cttgttccgc ggcatgagcg tgctcgacaa catcatgatg ggccgtcaca cgcacatgcg
     1801 tccctcggct ctggcaagct tgttctattg ggtgtgggcc gaacctgagg agatccggca
     1861 gcgggagttg gtggaggaca ccatcgactt tttgcaactc caggacgtgc gagacttgcc
     1921 cattgaggtc gtgccgctcg gcctgcagaa gcgcatcgag ctggcgcgcg cgctcgtctc
     1981 cgagccgcgt ctcctcatcc tcgacgagcc catggcgggc atgaaccagg aggagaagga
     2041 gtacatggcc cgcttcattc tcgacgcccg cgaggagcgc ggactcaccg tcttgctcat
     2101 cgagcatcac atggacgtgg tctcggcgct gtgcgatcgg atgaccgtgt tgagctacgg
     2161 cgagcagatc gcttcaggca cacccgcgga agcgctttcc aaccccgctg tgatcgaagc
     2221 ctatctcgga acgaagaggc gccatgcggc ctgaggggag cacagtcaaa gccgtcgatg
     2281 tgggtttgga tctcgccacg gatgatccgg gcgcggtgcc gcgcgagaat gtggggctcg
     2341 actccggcac cctgatctcg ttgctcgcca agaatgcgca gacgcatggg tccagtgtcg
     2401 ccatgcgcga gcgggaccgc ggcatctggc gagagttcac atggagcgac gtcctctccg
     2461 aggttctggc cttcgcggcg gctctcgatg cccacggttt ccagcctggt gatgggcttc
     2521 tggtgatcgg cgacaaccgc ccgcggctct atttcggtat gctcagcgcc gcggccctgc
     2581 gcggctatcc cgcgccggtt taccccgacg tgccaccaga cgacctcgaa tattacgccg
     2641 gcaacagccg tgcacggttc gcgctcgctg aggatcagga gcaggtcgac a
//
LOCUS       DASUMG010000127         2413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_337601, whole genome shotgun sequence.
ACCESSION   DASUMG010000127 DASUMG010000000
VERSION     DASUMG010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337601"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFX46_02055"
     CDS             complement(<1..627)
                     /locus_tag="VFX46_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5599398.1"
                     /translation="MRYWIGLAAIGIAAALPLQLDPMGYPIRVLTLAMLFAALGQAWN
                     IVGGLANQISLGHAAFFGLGAYTSTLLLINYGISPWLGMIAGAAVASVAALILSVPTM
                     RLQGHYFALATLAFSEVLRVIAISWSDVTGGPVGLSVPFSPDSFVQLQFRSTLPYYYI
                     ILAALVLVSVVFLLIRESALGYRLRAVKENVEAAEVIGVDTTRVKIIAA"
     gene            complement(624..1484)
                     /locus_tag="VFX46_02060"
     CDS             complement(624..1484)
                     /locus_tag="VFX46_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5599399.1"
                     /translation="MAFLQAVIDGLLIGGVYAVISSGLTLVFGVMGIINFAQAEFLML
                     GMFVAYFAWQLLGLDPLIGSLISFVVIFCLGVLVQMTLIQRVLRAPPVAQIFATVGLL
                     IVIENAALIAFGSDFRSVSTSYQTSALRLGPIFISVPYLAAFLMSLLSGCALWLFMNK
                     TWYGRAMRATAQDPMAANLMGIDADRMRQIAFGLGVGLTAFGGAVILPYFTVHPTVGQ
                     QFVVLMFTVVVLGGLGSVAGAVVGGLTVGVIQSVSTLFFPIQLQNLILFIVFITMLAL
                     RPTGLIGAAR"
     gene            complement(1568..>2413)
                     /locus_tag="VFX46_02065"
     CDS             complement(1568..>2413)
                     /locus_tag="VFX46_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599400.1"
                     /translation="EATERRGIPLLTMSFSDQITGRGFKYVFQVISKASTIGAAQLEG
                     TMEIAQGSGQKLSSIAIMYEDTAYGTSQAKGLRETAAKHNIEVVMDDAYPLGITDVTP
                     LINKLRATNAQAVFPCSYLNDGLQIIRSMRQQNITLPVIGGAGGYVIPDFKKGLGDFA
                     EGVLSVAPGNYDLAPELTNAFKERYGYFMVHEALEHAVCLEVLAQALEKAGKADPEAV
                     REALATTRFEGGWCNAMPGKAVDFDETGLNTLAKPLMVQWRGEDLVTVWPSDLAKAKA
                     VWGGA"
BASE COUNT          554 a          745 c          720 g          394 t
ORIGIN      
        1 ggcggcgatg atcttgacac gggttgtatc aacgccaatc acctccgccg cctcgacgtt
       61 ctccttcacc gcgcgcagcc gatagccgag ggcactttcg cgaatgagga gaaatacgac
      121 ggacacgagc accagcgctg cgagaatgat gtagtagtaa ggcaaggtgc tcctgaactg
      181 cagttgcaca aagctatccg gcgaaaacgg gacggaaagc ccaaccggac cgcctgtcac
      241 gtccgaccat gatatcgcga tgacacgcag cacctcgctg aatgccaggg tcgcgagcgc
      301 aaaatagtgc ccttgcagcc gcattgttgg aacactgagg ataagcgcgg ccacggatgc
      361 tacggccgca ccggcgatca tcccgagcca tggcgaaatg ccgtagttga tcaggagaag
      421 cgtcgaggtg taggcgccga gcccgaagaa agcggcatgg ccaagcgaaa tctggttcgc
      481 caatccgcca acgatattcc acgcctgccc gagcgctgcg aacaacatcg caagcgtcag
      541 cacgcggatt ggatagccca tggggtcgag ctggaggggc aatgccgcgg cgatgccgat
      601 cgccgcaagt ccaatccagt acctcatcgc gcagctccaa tgaggcccgt cggacggagc
      661 gccagcatcg tgatgaacac gatgaataga ataaggttct ggagctgaat ggggaaaaac
      721 aaggtcgaga cggactggat gacgccgacc gtaagccccc cgaccacagc gcccgccaca
      781 ctgccaagcc cgccgaggac caccacggtg aacatcagca ccacgaactg ctgccccacg
      841 gttggatgta cggtgaaata cggcaggatc accgcgccgc cgaaagccgt gaggccgacg
      901 ccaaggccaa acgcgatctg gcgcatgcgg tcagcatcga tgcccatgag attggcagcc
      961 atcggatcct gcgccgtggc ccgcatcgcc cgcccgtacc aagtcttgtt catgaagagc
     1021 cacagcgcgc agcctgatag gagcgacatc aggaatgcgg ccagataggg cacgctgata
     1081 aaaatcgggc ccagtctcag cgcggaggtc tgataggaag tcgagaccga tcgaaaatcc
     1141 gagccgaacg caatcagcgc cgcgttctcg ataacgatga gcagcccgac agtcgcaaag
     1201 atttgcgcca caggcggcgc gcggagcaca cgctggatga gcgtcatttg caccaggacg
     1261 cccaggcaga agatgacaac gaacgaaatg agcgagccga tcagcggatc gagccccagg
     1321 agctgccaag cgaaataggc aacgaacatg ccgagcatca ggaactcggc ctgtgcaaag
     1381 ttgatgatgc ccatgacgcc gaagacgaga gtcaggccgc tggagatcac ggcgtagacg
     1441 ccgccgatca acagcccatc gatcacggct tgaaggaagg ccaccaagaa gctccgagat
     1501 tggaccagct tcgaaacggc ttcaggtggg cacggtgcac cgaccggccc acctcgcgga
     1561 aggctaatta ggcgccgccc cagacggcct tcgctttggc aagatcactc ggccacacgg
     1621 tgacgagatc ctcaccccgc cactgcacca tcagcggctt ggccagcgta ttaaggcccg
     1681 tttcgtcgaa atcgactgcc ttgccgggca tggcattgca ccatccgccc tcgaagcgcg
     1741 ttgttgcaag ggcctcgcgg acggcctctg gatcggcctt accggccttc tcgagagcct
     1801 gggccagcac ctcgaggcac accgcgtgct cgagcgcctc atggaccatg aagtagccgt
     1861 aacgctcctt aaaggcgttc gtgagctctg gcgcgagatc ataattgcct ggcgcgaccg
     1921 acaagacacc ctcggcaaaa tctccgagcc ccttcttgaa atcaggtatc acgtagccgc
     1981 cagcgccgcc gatcacaggc agtgttatgt tctgctggcg catcgagcgg atgatttgca
     2041 gcccgtcatt gaggtacgag cacgggaaga cagcctgggc attggtggcg cgcagcttgt
     2101 tgatgagcgg cgtgacgtcg gtgatgccga ggggataggc atcgtccatc accacctcaa
     2161 tattgtgctt ggccgctgtc tcccgcaggc cctttgcttg cgaggttccg taggccgtgt
     2221 cctcgtacat gatggcgatg ctactgagct tttgcccgga gccctgtgcg atctccatcg
     2281 tcccttcaag ctgcgcagcg ccaatagttg atgccttcga gatcacctgg aacacgtatt
     2341 tgaacccgcg gccggtgatc tgatccgaga acgacatcgt gagtagcgga attccccggc
     2401 gctccgtcgc ctc
//
LOCUS       DASUMG010000128         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_417876, whole genome shotgun sequence.
ACCESSION   DASUMG010000128 DASUMG010000000
VERSION     DASUMG010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417876"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..842)
                     /locus_tag="VFX46_02070"
     CDS             complement(153..842)
                     /locus_tag="VFX46_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX5599401.1"
                     /translation="MTSRDAILSKIRAGLGAKVSEAERRQIVNDRLAARARHLIPARV
                     KEKSPHQLLALFRGFLERESATVLEVDSRQQIPSAIANYLRSNNLPARIRVGGDTMLT
                     QLPWGSEPHLEMLLGRAQPNDGVGLSRAAAAVAETGTLVMASGPDNPVTLNFLPETHI
                     VVVEQANLVPAYEDAWDLIRARFGPNGMPRTVNFISGPSRTADVGGKLVMGAHGPRRL
                     CVIVVKEPASS"
     gene            complement(839..2266)
                     /locus_tag="VFX46_02075"
     CDS             complement(839..2266)
                     /locus_tag="VFX46_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HEX5599402.1"
                     /translation="MKPTTKSFKQNVRVALTDAQLQRALAGLPTGLVANRAAARARLP
                     EFDDLREIGRDIKDHTLAHLDLYLEEYERNATAAGAKVHWAATAAEAREIILNICQEV
                     NAKIVTKGKSMITEEIGLNAELQAAGMEVVETDLGEYLVQIRGESPSHIIAPAIHLTQ
                     DQVEADFRRLHTNLPKDRVLDEAAQLVGEARQVLREKFLAADVGITGANFLIAETGSS
                     VIVTNEGNGDLTQSLARVHVAVASIEKIVPTREDVSAILRLLARSATGQEISTYTTFS
                     TGPRRADDPDGPEAYHVVILDNGRSELLGTAFREVLRCIRCGACMNHCPVYAAIGGHA
                     YGWVYPGPIGAVLTPALIGVREAADLPNASTFCGRCEQVCPMKIPLPKLMRHHREAEF
                     ELGGPSRRFRLGLKAWAWLATRPGLYHVTMRLAAAFLGALGRRRGAFRWLPLTRGWTQ
                     HRDFPAPQGRTFQQLWAEHKAGAPR"
     gene            complement(2352..>2582)
                     /locus_tag="VFX46_02080"
     CDS             complement(2352..>2582)
                     /locus_tag="VFX46_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5599403.1"
                     /translation="GGTFAVKYPDISNAMVEKKTANVAATDPDLLLAGDLGCLLNMAG
                     KLKREGRRIAARHVAEVLAGEISDPPIAGSGK"
BASE COUNT          429 a          818 c          852 g          483 t
ORIGIN      
        1 tgccgcgccc agtacaatca gtgccgcatt gcgaccaagg ggtcgtctgc ctgcgatgct
       61 cagttcgccg cctgcatgag gcgctgcatc tcaagtcgga agtgacctcc cgaattggct
      121 cgcgaaccag cagtctccaa ggtttcggca ggtcaactgc tggcgggctc tttcaccacg
      181 atgacgcaca ggcggcgcgg accgtgagcg cccatgacga gcttgccgcc aacatctgcg
      241 gtgcgtgacg ggccggatat gaaattgacc gtcctcggca tcccattcgg gccaaaccgg
      301 gcgcgaatga ggtcccacgc atcctcgtag gcaggcacga gattggcctg ctcgacgacg
      361 acaatgtgtg tttccggaag gaaattgagg gtcaccgggt tgtctggtcc cgatgccatc
      421 accagcgtgc cggtttcggc taccgcggct gccgcccggg ataggccaac accgtcattg
      481 ggttgtgcac ggcccagcag catttcgagg tgtggctctg aaccccaggg gagctgggtg
      541 agcatggtgt cgccgcccac acggatgcgt gctggcaggt tgttcgaccg cagatagttg
      601 gcgatcgcgg aaggaatttg ctgcctcgag tcgacttcca gcaccgtggc ggattcgcgc
      661 tccaggaaac cgcggaacag cgcgagcagc tggtgaggtg acttctcctt gacgcgtgcg
      721 gggatcaggt ggcgtgcgcg agccgcaagg cgatcattga cgatctgccg ccgctcggcc
      781 tcgctcacct tggcaccgag gcccgcccgg attttcgaca ggatggcgtc gcggcttgtc
      841 atctcggtgc tcccgccttg tgctccgccc agagctgttg gaacgtccgg ccctgtggtg
      901 ctgggaaatc gcggtgctgc gtccaccccc ttgtcagcgg cagccagcgg aacgcgcccc
      961 ggcggcggcc gagcgcaccc aggaaagccg cagccagccg catcgtcacg tgatagaggc
     1021 ccggccgggt ggccagccat gcccaggctt tgaggccgag ccggaagcgg cgcgacggtc
     1081 cccccagctc gaactcggcc tcgcgatggt ggcgcatgag cttcggcagc ggaattttca
     1141 tcgggcagac ctgttcgcag cgcccgcaaa aggtcgaggc attgggcaga tccgctgcct
     1201 cgcggacgcc gatcagcgct ggcgtcagca ccgccccaat cggtcccgga taaacccagc
     1261 cgtacgcgtg cccaccgatg gccgcataga cggggcaatg gttcatgcag gcgccgcaac
     1321 ggatgcagcg cagcacctcg cggaaggcgg tcccaagcaa ctccgagcgg ccgttgtcca
     1381 gaatgaccac gtggtaggct tccggaccat caggatcatc agcccggcgc ggtccggtgg
     1441 agaaggtggt gtaggtggag atttcttgcc ctgtcgccga acgcgcgagc aggcggagaa
     1501 ttgcgctcac gtcctctcgc gtgggcacga ttttctcgat ggaggcgacg gccacgtgca
     1561 cgcgggcgag cgactgcgtc aggtcaccgt tgccctcgtt tgtgacaatt accgacgatc
     1621 cggtttcagc gatgaggaaa ttggcgccag tgatgcccac atcggcggcc aggaacttct
     1681 cccgcagcac ctgtctggcc tcgccgacga gctgggccgc ctcgtccaga acgcgatcct
     1741 tcggaaggtt ggtatgcagg cggcggaaat cggcctcgac ctggtcctgg gtcaaatgga
     1801 tggcgggcgc gatgatgtgg ctcggtgatt cgccgcggat ctgcacgagg tattcgccga
     1861 gatcggtctc caccacctcc atgccagcgg cctggagctc ggcattgagg ccgatctcct
     1921 ccgtgatcat ggatttgccc ttggtgacga tcttggcgtt tacctcctgg caaatgttga
     1981 ggatgatctc gcgcgcctcc gcggccgttg ctgcccagtg caccttcgct cccgccgctg
     2041 tggcgttccg ttcgtactct tctaggtaga ggtcgaggtg cgcgagcgta tggtctttga
     2101 tgtctcgccc gatctcgcgg aggtcgtcga actctggcaa acgggcgcgg gcagcggcac
     2161 ggttcgcgac gaggccggtt ggcagccccg ccagcgcccg ttggagctgc gcatcggtca
     2221 gcgcgacgcg cacattctgc ttgaaggact tcgtcgtagg cttcatcggg cgatcctttt
     2281 gccggcgtca ggcgtgcggc gatcgtcagc ttggcgcgaa cgagatctgg gaagcaaggc
     2341 caagggggtg ctcactttcc tgacccggcg atcggcgggt cggagatttc gccggcaagc
     2401 acttccgcca catggcgagc cgcgatccgg cgcccttcgc gcttgagctt cccagccatg
     2461 ttcaacaggc acccgagatc gcctgcgagc aacagatcgg ggtcggtcgc tgccacattg
     2521 gccgtcttct tttcgaccat agcgttggag atgtccggat acttcacagc gaacgtgccg
     2581 cc
//
LOCUS       DASUMG010000129         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_980512, whole genome shotgun sequence.
ACCESSION   DASUMG010000129 DASUMG010000000
VERSION     DASUMG010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980512"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFX46_02085"
     CDS             complement(<1..306)
                     /locus_tag="VFX46_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5599404.1"
                     /translation="MINNMDVQKLGQESFDRAMRLLGDWNKGWQAIAAEMTDYSKRSI
                     EQGTKTFEKLLAAKSMEQAIEIQTNYARRAYDDYMQQITKLSGMYANLAKEAYKPLRT
                     "
     gene            656..2275
                     /locus_tag="VFX46_02090"
     CDS             656..2275
                     /locus_tag="VFX46_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX5599405.1"
                     /translation="MRLTELLCIARRWQAIFLLLATLFVAVAAPSAVAAEKRASMVID
                     ANTGKTLHASRADELRHPASLTKIMTLYLVFEEIEQGRLSLDSKIRMTKWGAMAPPSK
                     LGLKEGETISVRDAIRALVTKSANDVARAVAEHISGTEANFAKMMTEKARLLGMSKTT
                     FRNASGLPDPEQVTTARDMLTLALHLQDHFPQHYHFFSTRVFNFKGRSYRNHNGLLRT
                     YAGTDGIKTGYIRMSGFNVVTSVRRGGRHVVGVVFGGKTAASRNAEMRALLDRALGKA
                     STKKTRQPVAVSFYRATPPKPAQRPARATQVAASPTPRPIKVSDTIPAEPQHPTTPAK
                     ATDAASSTPKITVAKVRPVSITPVGGSQPEQAAQAEPKSTSNLPTALSDSAGTTGVVL
                     GRPPSTLQEQAARLSHGQPALAGFGFMSQAHAMPAPPPSAQRAAPPPAREHSQSQSSE
                     APPRMPDAGGYHVQVGAYTSLAEAEKALVATLEQAPTVLAEAAPRTIPVVSGSRRLYR
                     ARFVGLDSSAARNACEELRRRKIDCFVAKAE"
     gene            complement(2311..>2719)
                     /locus_tag="VFX46_02095"
     CDS             complement(2311..>2719)
                     /locus_tag="VFX46_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5599406.1"
                     /translation="MELDHDTGAMRGRVLKGTFAGREIESMSPADLAMLWQECRFADP
                     KSAQIIEAYLDTAHPSWRDDMARAGADPGHAASGRMTREEAYEILGLEPGATEEDIRR
                     AHRELMMKLHPDRGGSAYLAAKINLAKEVLLGR"
BASE COUNT          499 a          901 c          808 g          511 t
ORIGIN      
        1 cgtgcggagg ggcttgtaag cctccttggc gaggttcgcg tacatgccgc tgagcttcgt
       61 tatctgctgc atgtagtcgt cgtaagcgcg cctcgcgtag ttcgtttgaa tctcgattgc
      121 ttgctccatg ctcttggctg cgaggagctt ttcgaatgtc ttggtcccct gctcgattga
      181 gcgcttacta taatctgtca tctcggcggc aatggcctgc cagcctttgt tccagtctcc
      241 caagagacgc atagcacggt cgaagctctc ctggccgagc ttttgcacgt ccatgttgtt
      301 gatcatgacg caatcctgac aattgagggg gctgcggggg cagcatcgcg agttggcgct
      361 gcacaagata cgcctgtcag atactacgtc aagtcggatg cctgggctgc cgcaaccctc
      421 gctggcagac gttaaccaga tcctagtccc acctgtctag gctcaccact cgccttgaag
      481 ctgataatcg gccggcggct gagcgccgca aaggcgccaa aaaccgctgt ttcggtggac
      541 gcgaatgcaa cagcccgagc tcgagtgtgg cggttctgag cccggtcctg cgctcgccag
      601 cggatccgca ttgcgatgcc gcagccacgg gggctcttct tgtaacgagt acgcgatgcg
      661 gctgaccgaa ctcctttgca ttgcccggcg atggcaggcc attttcctcc tgctggccac
      721 gctattcgtg gccgtggcgg cgccatctgc tgttgccgcc gagaagcggg catcgatggt
      781 catcgacgcc aataccggca agacgttgca cgcctcgcgg gcggatgagc tacgccatcc
      841 cgcttccctc acgaagatca tgaccctcta tctggtcttc gaggagatcg agcagggccg
      901 cctcagcctc gactcgaaaa tccgcatgac gaagtggggc gccatggcgc cgccatccaa
      961 gctcggcctc aaggaaggcg agacgatttc ggtacgcgat gcgatccgtg ccctggtgac
     1021 gaagtcggcc aacgacgttg cccgagccgt tgctgagcac attagtggca cggaggcgaa
     1081 cttcgcgaag atgatgaccg agaaggcccg acttctcggc atgtcgaaaa ccacgttccg
     1141 gaacgcatcg ggcctgcccg accccgagca ggtgacgacg gcgcgcgata tgctgacgct
     1201 ggcgctccac ctgcaggacc acttcccgca gcactatcac ttcttctcga ctcgggtgtt
     1261 caatttcaaa ggccgcagct accgcaatca caatggcctc ttgcgcacct acgcaggaac
     1321 ggacggcatc aaaaccggat acattcgcat gtcgggcttc aacgtcgtca cctcggtgcg
     1381 gcggggcggg cgccacgtcg ttggcgtcgt attcggcggc aagacggctg cgagccgcaa
     1441 tgcggaaatg cgagcgttgc tcgatcgcgc actcgggaag gcttccacca agaagacccg
     1501 ccaacccgtg gccgtctcct tctatcgtgc cacgcctccc aaaccggcac agcggcctgc
     1561 tcgcgcaacc caggtggcag cgtcgcccac tccgagaccc atcaaggttt ccgacaccat
     1621 acccgctgag ccgcagcacc cgaccacacc agccaaggca accgacgctg cttcgtctac
     1681 gcccaagatc accgttgcga aggtgcgccc ggtctcgatc acgcctgtcg gaggatcaca
     1741 gccagagcaa gccgctcagg ccgagcctaa gtcaacatcc aatcttccga ctgcattatc
     1801 ggattcagca ggcacgaccg gagttgtgct ggggcgtccc ccttccactc tccaggagca
     1861 ggcagccaga ctatctcacg gccagccggc actggcgggg tttggcttca tgagccaagc
     1921 ccatgccatg ccggcaccgc cgccctctgc ccaaagagca gctccgcctc cggcgcgaga
     1981 acactcgcaa tcacaatcca gtgaggcgcc cccaagaatg cccgatgccg gcgggtacca
     2041 tgtccaggtc ggggcttata cgtcgctggc cgaagcagag aaggccctgg tcgcgacgtt
     2101 ggagcaggca cccacggtgc ttgccgaagc cgccccaagg accatccctg tcgtgagcgg
     2161 ctcccggcgg ctttacagag cccggttcgt cggtctcgac tcctcggcgg cacgcaacgc
     2221 ctgcgaggag ctgcgccgcc ggaagatcga ctgcttcgtt gccaaggctg agtagccggg
     2281 gatagaagcc atccacctca cggctttttg ctagcgcccg agcaacacct cttttgcgag
     2341 attgatcttg gcggcgagat acgcggagcc gccgcgatcc gggtgcaact tcatcatgag
     2401 ctcgcgatgg gcgcggcgga tgtcctcctc ggtggcaccc ggctcgaggc ccagaatctc
     2461 gtaagcctcc tcacgggtca tccttcctga cgccgcatgg ccaggatcgg ctccggctcg
     2521 cgccatatcg tcccgccagc tggggtgcgc ggtgtcgaga taggcctcga tgatctgtgc
     2581 agatttggga tcggcgaacc ggcactcttg ccacagcatg gcgaggtcag ccgggctcat
     2641 gctctcgatc tcccggcctg cgaaggtgcc cttgagcacg cgaccgcgca tggcgcccgt
     2701 gtcgtggtcg agctccatc
//
LOCUS       DASUMG010000130         3731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_980843, whole genome shotgun sequence.
ACCESSION   DASUMG010000130 DASUMG010000000
VERSION     DASUMG010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_980843"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..845
                     /gene="purC"
                     /locus_tag="VFX46_02100"
     CDS             57..845
                     /gene="purC"
                     /locus_tag="VFX46_02100"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX5599407.1"
                     /translation="MSKRRRVYEGKAKVLYEGPEPGTLIQHFKDDATAFNNKKHALIE
                     GKGVLNNRISEYIFVKLGEIGVPTHFIRRLNMREQLIREVEIIPLEVVVRNVAAGSLA
                     QRLGLEEGMQLPRSIVEFYYKADNLNDPLVSEEHITAFGWATPHEIDEIMQLALRIND
                     FLVGLFLGIGIRLVDFKVEFGRLWDNDQMRIVLADEISPDCCRLWDIRTSEKLDKDRF
                     RRDMGGLLEAYQEVARRLGVFTDNRPVKGVVPVLVASNPGGKPN"
     gene            941..1183
                     /gene="purS"
                     /locus_tag="VFX46_02105"
     CDS             941..1183
                     /gene="purS"
                     /locus_tag="VFX46_02105"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEX5599408.1"
                     /translation="MKARIIITLKNGVLDPQGKAIENAVAALGIGGVQSVRQGKFIEV
                     ELAASSQAEARETVERMCRELLANTVIENYRYEIEG"
     gene            1230..1928
                     /gene="purQ"
                     /locus_tag="VFX46_02110"
     CDS             1230..1928
                     /gene="purQ"
                     /locus_tag="VFX46_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5599409.1"
                     /translation="MKASVIIFPGTNREHDVADVLESVSGRKPQFVWHSETQVPPTDL
                     IVLPGGFSYGDYLRCGAMAARSPIMRDVIAKAKAGTPVLGICNGFQILCESGLLPGVL
                     MRNASLMFVCKDVWLRVEQPDTIFTSRFARGEVMPAIAAHAEGNYFADPDTLDRLEGE
                     GRIVFRYADRHGNVTPEANPNGSQRNIAGICDERRRVLGLMPHPENASDPLLGPTGGR
                     KLFESLAESLVGTA"
     gene            complement(2110..2586)
                     /gene="greA"
                     /locus_tag="VFX46_02115"
     CDS             complement(2110..2586)
                     /gene="greA"
                     /locus_tag="VFX46_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5599410.1"
                     /translation="MTERVPMTAEGYRQLEAELHRLKSVERPRIIQAISEARSHGDLS
                     ENAEYHAAKEAQGMNEARVAELEDKLSRAEVIDPKTLSGDTVKFGATVTLVDEDTEEE
                     VKYMIVGEFEANVREGRISIGSPIARALIGRSKGDSVEVTTPKGTRSYEIVNVEFK"
     gene            complement(2705..>3731)
                     /locus_tag="VFX46_02120"
     CDS             complement(2705..>3731)
                     /locus_tag="VFX46_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5599411.1"
                     /translation="ATLDRPSELVVSERRPLLIDRYLSDAIEVDVDCIADGKDTFVAG
                     IMEHIEEAGIHSGDSACALPPHSLDAETITELERQTRELALALNVVGLMNVQFAIKNG
                     EVYILEVNPRASRTVPFVAKVIGLPIARIAAQVMAGRPLASFELKQRKLDHIGVKEAV
                     FPFARFVGVDPILGPEMRSTGEVMGLDRDFAMAFGKSQIASGQALPTSGTVFVSVKDS
                     DKDRIVAPVRDLVEMGFRIVATRGTKRHLEQHGVPCTLVNKVLEGRPHVVDAMKNGEI
                     SIVFNTTEGARALADSKDIRRTALLNHIPYYTTLAGAIAVTRAIKALQSDTLKVAPLQ
                     SYAERPA"
BASE COUNT          727 a         1189 c         1118 g          697 t
ORIGIN      
        1 aacgtccggg aagcgttcct ctcctgccca gaccagctat tgcgaggaca acaagaatga
       61 gcaagcggcg ccgtgtctat gagggcaagg ccaaggtgct ctacgagggg ccggagcccg
      121 gcaccctgat tcagcacttc aaggacgatg ccacggcctt caacaacaag aagcacgcgt
      181 tgatcgaagg caaaggcgtg ctcaacaacc ggatttccga gtacattttc gtgaagctcg
      241 gcgagattgg cgtgccgacg cacttcattc gccgcctcaa catgcgcgag cagctcattc
      301 gcgaagtgga gatcatcccg ctcgaggtcg tcgtgcgcaa cgtggccgcc ggctcgctcg
      361 cccagaggct gggcctcgag gagggcatgc agctcccccg ctcgatcgtc gagttctact
      421 acaaggcgga caatctcaac gacccgctcg tctctgagga gcacatcacg gccttcgggt
      481 gggcgacgcc gcacgagatc gacgagatca tgcagctcgc gctgcggatc aacgacttcc
      541 tggtgggcct gttcctcggc attggcatcc ggctcgtcga cttcaaggtc gagttcgggc
      601 ggctgtggga caacgatcag atgcggatcg tccttgccga cgagatcagc ccggattgct
      661 gccggctgtg ggatatccgg acatccgaaa agctcgacaa ggaccgcttc cgccgcgaca
      721 tgggtggatt gctcgaggcc taccaagagg ttgcccgccg cctcggcgtg tttaccgaca
      781 accggccggt gaagggggtc gtgccggtgc tggtagcctc caatcccggc gggaagccga
      841 actgagcata acttcggcct gaagagcagc gaatcattgc cgaggacaac ggcatgagct
      901 actcgcgtcc ccggcacgac actcaccttt cgcttgtgaa atgaaagctc gcatcatcat
      961 caccttgaag aacggcgttc tcgatccgca gggcaaggcc atcgagaacg ccgttgccgc
     1021 gctggggata ggcggggttc aatccgtccg gcaaggcaaa ttcatcgaag tggagcttgc
     1081 tgcgagcagc caggcggaag cccgtgagac agtcgagcgc atgtgccgcg aacttctcgc
     1141 gaacacggta atcgagaact ataggtacga gatcgagggc tgatacaatc gatcgggcct
     1201 agcctgattt cctcgcacag cagggcggca tgaaggcttc cgtcatcatt tttcctggaa
     1261 ccaatcgcga acacgacgtc gcggacgtgt tggagtccgt ctccgggcgc aaaccccagt
     1321 tcgtctggca cagcgagacg caagtcccgc caacggattt gatcgtgctg cccggcgggt
     1381 tctcctacgg cgactacctg cgctgtggcg ccatggccgc gcgctcgccc atcatgcggg
     1441 acgtgatcgc caaggccaaa gccggcacac cggtgctcgg catctgcaat gggtttcaga
     1501 tcctttgcga atccgggctt cttccgggtg tgctgatgcg gaacgcttcg ctcatgttcg
     1561 tctgcaagga tgtatggctc cgggtggagc agccggacac gatcttcaca agccgcttcg
     1621 cgcgcgggga ggtcatgcct gccattgccg cccacgcgga gggcaactac ttcgccgatc
     1681 ctgacaccct cgaccgcctc gagggcgaag gccgcatcgt gttccgctat gccgaccggc
     1741 acggaaatgt aacgccggaa gccaacccca acggatccca gcgcaacatt gccgggatct
     1801 gcgacgagcg ccgccgcgtc ctcggcctga tgccgcatcc ggaaaatgcg tcagacccgc
     1861 tgctcgggcc gaccggcggc cggaagctgt tcgagagcct cgccgagagc ttagtgggga
     1921 ctgcctagaa ggttaagccc cctccccttg gggaacgccg tgagggctgg acttcctcca
     1981 gccgtaaagc cgcttccctc acgaaccgat gtccatccca aatgaaaggg gcccggatcg
     2041 ggcccccagc tccgatgccc ctggctacga caggaaaatg gatggaacag atcgcctaac
     2101 gttgcacccc tatttgaact cgacgttcac aatctcatag gaccgggtgc ccttgggcgt
     2161 cgtcacttcg accgaatcgc ctttggatct gccgatcagc gcgcgggcga tcggcgagcc
     2221 gatggaaatg cggccctcgc gcacgttggc ctcgaactcg ccgacgatca tgtacttcac
     2281 ctcttcctcg gtgtcctcgt cgacgagtgt gacagtggcg ccgaacttga ccgtgtcgcc
     2341 gctcagcgtc ttgggatcaa tgacctcggc acgggaaagc ttgtcctcaa gctccgccac
     2401 cctggcctcg ttcatcccct gcgcttcctt ggcggcgtga tactcggcgt tctcactcag
     2461 gtcgccgtgc gagcgcgctt cgcttatcgc ttggatgatg cgcggccgct ccacagactt
     2521 cagccgatgc agctcggcct caagctgccg atagccttct gcggtcatgg gcacgcgttc
     2581 ggtcatagtc aaagcctcct caccgacgca gactgcgccg gagcatcgcc tgtagccgtt
     2641 tcactcacca gggaaagtta ggtcagcccc aatgcgcaac aagctaatgc acgtaggccc
     2701 gcattcaagc gggcctttca gcatagctct gcaggggcgc gaccttcaat gtatcggact
     2761 gcaacgcctt gatggctctc gtcactgcga tcgcaccggc aagagttgta taatacggaa
     2821 tgtgattcag caaggccgtt cgccgaatgt ccttggagtc ggcaagggcc cgcgccccct
     2881 cggtcgtatt gaacacgatc gagatctctc cgttcttcat agcgtcgacg acgtggggcc
     2941 gcccctccag caccttgttg acgagagtac aggggacccc atgctgctca aggtgccgct
     3001 ttgtgccccg cgtcgctacg atacggaagc ccatttcgac cagatcccgc acgggtgcca
     3061 cgatccgatc cttatccgag tccttcaccg agacgaatac ggtccctgac gtcggcaagg
     3121 cttgtcccga ggcgatctgg ctcttcccga aggccatcgc gaagtcgcgg tcgagaccca
     3181 tgacctcgcc ggtcgatctc atctcgggcc cgaggattgg atcaaccccg acaaagcgag
     3241 cgaaggggaa tacagcctct ttgacaccga tgtggtcaag cttgcgctgc ttcaattcaa
     3301 aggaggcgag cggcctgccc gccatcacct gggcagcgat gcgggcgatc ggaagcccga
     3361 tcaccttggc gacgaagggc accgtacgac tggcgcgcgg attaacctcc aagatgtaga
     3421 cctcgccatt cttgatggcg aactgcacgt tcatgagccc gacgacgttg agcgccagcg
     3481 cgagctctcg agtctgccgc tcaagctctg tgatcgtctc cgcgtcgagt gagtgaggcg
     3541 ggagggcaca agccgaatcg cccgagtgga tgccagcctc ctcgatgtgc tccatgatgc
     3601 cggcaacgaa cgtgtccttt ccgtcggcta tgcagtcgac gtcaacctcg atggcgtcgg
     3661 aaaggtaccg gtcgatcagg agtggtcgcc gctccgacac gacaagctcg gatggccggt
     3721 cgagcgtcgc c
//
LOCUS       DASUMG010000131         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1060723, whole genome shotgun sequence.
ACCESSION   DASUMG010000131 DASUMG010000000
VERSION     DASUMG010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060723"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="VFX46_02125"
     CDS             <1..1240
                     /locus_tag="VFX46_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5599412.1"
                     /translation="GRSAMPAERLEKKLEERLAELGRTGRLKGDEAVITKVIPPADGK
                     GPRFFLEGYGAQPFLRMNSNSYLGLSLDAEVKAAEDEAVREYGTGPGAVRFISGTWAP
                     HVALERRLAEFHGREAAMLFSSAYATVMGLLPQLITDRTAVISDELNHNCIINAIALG
                     RPAEKRIWKHLDLDDLERHLKEAAQKCDRAIVVTDGVFSMRGDHAPLDAIRKIIDKFD
                     SAFAENAILIVDDSHGVGAFGPTGRGTEEVTGARADLLVGTLGKAFGVNGGYVTGSAT
                     IIRYLRETSPFYIYSNPITPAEAAAALKAVEIVDSPRGQQLLGHLREMTARFRHGLTV
                     LGLETLPGEHPVVPLMVRDTTRTAVLVAHLRNAGILATGLNYPVVPRGDEEIRFQICA
                     DHTPADIDEVLAALGAFTGR"
     gene            1310..1738
                     /locus_tag="VFX46_02130"
     CDS             1310..1738
                     /locus_tag="VFX46_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5599413.1"
                     /translation="MDADRIIELLELEPHPEGGYFRETFRDARVVEGRSCSTAIYFLL
                     KEGQVSHWHRVDAVEVWHWYAGAPLELGIAPDGGPEQIVRLGPSLFAGERPQVVIPAH
                     HWQRARSLGRWTLVGCTVAPGFEFSGFELAGPDFHPSQRR"
     gene            complement(1902..>2289)
                     /locus_tag="VFX46_02135"
     CDS             complement(1902..>2289)
                     /locus_tag="VFX46_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5599414.1"
                     /translation="MRGRNSTASWLVGASIAAALLLGGLGGAAAQDTEEERVPPPPLP
                     EEVLKDPKVISTGEELWKDQCAHCHGSRAYPGKAPKLQPRRYKPEFVWDRVYNGFRGM
                     PPWKDVYTEEEIIAIVAYVMSDDFFP"
BASE COUNT          427 a          704 c          722 g          436 t
ORIGIN      
        1 cgggaggagc gccatgcctg cggaaaggct ggagaaaaag cttgaagagc gtctggccga
       61 gcttggccgc accgggcgtt tgaaaggtga cgaagcggtc atcaccaaag ttattccgcc
      121 agccgacgga aagggaccgc gcttctttct ggaaggctac ggcgcacagc cattcctgcg
      181 catgaattcg aacagctatc tcgggctgtc actcgatgcg gaggtgaagg cggctgaaga
      241 tgaggcagtc cgtgagtacg gcactggccc tggagcggtg cggttcatca gcggcacgtg
      301 ggcgccccat gtcgcgctcg agcgccggct tgcggagttt catggccggg aagccgctat
      361 gttgttcagc tccgcctacg ccaccgtgat gggcctgctg ccgcagctca ttaccgaccg
      421 gacagcggtg atcagcgacg agctcaatca taattgcatc atcaacgcca ttgcgctcgg
      481 gcgacccgcg gaaaagcgca tctggaagca tctcgacctc gacgatttgg agcggcacct
      541 caaggaagct gcccaaaagt gtgatcgggc gatcgtcgtg actgacggcg ttttcagcat
      601 gcgcggtgat cacgcgccgc tcgacgcaat tcggaagatc atcgacaaat tcgattccgc
      661 cttcgccgaa aacgcgatcc tgatcgttga tgactcgcac ggcgtcggcg cgttcgggcc
      721 aacgggccgg ggcacggagg aggtgacggg cgcccgggcg gatctactgg tcggaacgct
      781 cggaaaggcg ttcggcgtca atggcggcta cgtcacgggt agcgcgacga tcatccgata
      841 tttgcgggaa acctcgccat tctacattta ctcgaacccg attacgccgg cggaggccgc
      901 cgccgcgctc aaagccgttg agatcgtcga cagcccgcgc gggcagcagc tccttggtca
      961 cctccgtgag atgacggccc gcttccgtca cggtctcacc gtcctcggcc tcgaaacgct
     1021 gcccggcgag cacccggtcg tgccgcttat ggtgagagat acgacgcgca cggccgttct
     1081 cgtcgcgcat ctgcgcaacg ccggcattct ggcgactggg ctcaattatc cggttgtccc
     1141 ccgtggggat gaggagatcc gcttccagat ctgcgcggac catacacctg ccgacatcga
     1201 cgaggtgctg gccgcgctgg gcgccttcac cggccgctga catgcgcccg gagcgctgat
     1261 acggtgaccg cgcttgagca cggcggggac tgatatgcgc gacggcgata tggatgcgga
     1321 ccggatcatc gagttgttgg agctcgaacc gcacccggaa ggcggctatt tccgggagac
     1381 gtttcgggat gcaagagtgg tcgaaggccg gtcctgctcg acagcgatct actttctcct
     1441 gaaagagggg caggtctcgc actggcatcg cgtcgacgcg gtcgaggtct ggcactggta
     1501 tgctggcgcg ccgctcgagc ttggcatcgc gccggacggt ggccccgaac aaattgtccg
     1561 gctcggccca tcactctttg cgggcgagcg cccgcaggtc gtcattcctg cgcatcattg
     1621 gcagcgtgcc aggagcctcg gccgctggac gctcgttgga tgcacggttg cgccaggctt
     1681 cgaattctcc ggcttcgagc tcgccggccc cgattttcac ccgagccagc ggaggtaatt
     1741 cagcaatctg ctggaacgat acgtaagatc ttgtcgtgac cgccgagcgg acgaaggtta
     1801 ttcgctagca cgatggcccg caatcgtacc agcctcactt caaaagtcga attgtggctg
     1861 atcaggtggg cgaactgcgc tggacccgca gccccactat gtcacgggaa gaagtcgtcg
     1921 ctcatgacgt aggcaacgat cgcaatgatt tcctcctctg tatagacgtc tttccacggc
     1981 ggcatgccgc gaaaaccgtt gtaaacacgg tcccatacga attctggctt atagcgacgc
     2041 ggctgaagct tcggcgcttt ccccggatag gcacgcgacc cgtggcaatg ggcacattgg
     2101 tccttccaca gctcttcgcc cgtactgatg accttcgggt ccttgagcac ctcctccggt
     2161 agtggtggtg ggggcaccct ttcctcttcg gtatcttggg cagcagcgcc gcccagaccg
     2221 ccgagcaata acgccgcagc gattgacgcg cctacgagcc aggaagcggt agaatttcga
     2281 cctctcatc
//
LOCUS       DASUMG010000132         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_418427, whole genome shotgun sequence.
ACCESSION   DASUMG010000132 DASUMG010000000
VERSION     DASUMG010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418427"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="ccoO"
                     /locus_tag="VFX46_02140"
     CDS             <1..594
                     /gene="ccoO"
                     /locus_tag="VFX46_02140"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEX5599415.1"
                     /translation="MRPYTPLEQAGRDIYIREGCYTCHSQMIRTLRDEVERYGHYSLA
                     AESMYDHPFQWGSKRTGPDLARVGGKYSDQWHVEHLIDPRSVVPESIMPAYPWLTRKL
                     DAENIQERLKTLRAVGVPYTDEMIEKAFDDLKAQVDPDSRAARELLERYPKAKVAKFD
                     GRPGGEISELDALIAYLQVLGTLVDFASFDAAGPNLR"
     gene            606..785
                     /locus_tag="VFX46_02145"
     CDS             606..785
                     /locus_tag="VFX46_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018072402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX5599416.1"
                     /translation="MTYDTVATFSQVTSLFFFIALFIGVVVYAFWPSNRRRFEAAQRE
                     ALDLDEDTASHGGRK"
     gene            782..1648
                     /gene="ccoP"
                     /locus_tag="VFX46_02150"
     CDS             782..1648
                     /gene="ccoP"
                     /locus_tag="VFX46_02150"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX5599417.1"
                     /translation="MSRKEIDEVTGVETTGHEWDGIKELNKPLPKWWLWTFYATIIWS
                     FGYWVLYPAWPTMSGYTKGVLDYSQRAVVTEQVEAARAEQAKFRDALAATPLDQVKDD
                     PELLRFAMAGGATVFADNCAPCHGRGAQGFPGYPNLNDDEWLWGGTIEDIHTTIRFGI
                     RSGHEEGRESQMPRFGIDQLLDRNQISDTAEYVLSLSGRATDEAAVARGREIYAEQCV
                     ACHGEDGKGNVELGAPNLTDAIWLYGSSKEDIVESIRTGRGGQMPPWEGRLDPVTIKS
                     LAVYVHSLGGGQ"
     gene            1839..>2281
                     /locus_tag="VFX46_02155"
     CDS             1839..>2281
                     /locus_tag="VFX46_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX5599418.1"
                     /translation="MPQNLKPADFINEAAPGVEKHDVAAINRKDRRELYAARQKVYPR
                     LAYGRFRNIKWLVMAITLSIYYVLPWLRWDRGPNLPDQAVLLDFANGRLFLFFLEIWP
                     QELYYITGILVISALGLFLVTALAGRVWCGYACPQTVWTDLMIAV"
BASE COUNT          437 a          687 c          725 g          432 t
ORIGIN      
        1 atgcggccct acaccccgct cgagcaggcc ggccgggata tctacatccg cgaaggctgc
       61 tacacctgtc acagccagat gatccgcacg ctgcgtgacg aagtggagcg ttacggccat
      121 tacagcctcg ctgcggagag catgtacgac catccgttcc agtggggctc gaagcggacg
      181 gggcccgatc tcgctcgcgt cggcggtaag tactccgacc aatggcacgt tgagcacttg
      241 atcgatccgc gctctgtcgt gcccgagagc atcatgccgg cgtatccctg gctcacccgc
      301 aagctcgatg cggagaacat tcaggagcgg ttgaagacgc tgcgggctgt cggcgttccg
      361 tacaccgacg agatgatcga gaaggccttc gacgacttga aggcccaggt cgaccccgac
      421 tcgcgggcgg cgcgcgagct gctcgagcgc taccccaagg ccaaggtcgc caagttcgat
      481 ggccggccag gaggcgagat cagcgagctt gatgcgctga tcgcctacct gcaggtgctc
      541 ggcacgctgg tcgatttcgc cagcttcgat gccgccggtc ccaacctgag gtgagggcca
      601 gcgtcatgac ctacgacact gtggcgacat tcagccaagt cacttcgctg ttctttttca
      661 tcgcgctgtt catcggggtc gtcgtctacg ccttctggcc cagcaatcgc cggcgcttcg
      721 aagcagcgca acgtgaggcg ctcgatctcg atgaagacac cgcttcccac gggggccgca
      781 aatgagcagg aaagaaatcg acgaggtcac cggtgtcgag accaccggtc acgagtggga
      841 cggcatcaag gagctgaaca agccccttcc caagtggtgg ctttggacgt tctacgccac
      901 catcatctgg tccttcggct actgggtgct ctatccagcc tggccgacga tgagtggcta
      961 tacgaagggt gtgctcgatt atagtcagcg ggcggtagtg accgagcagg tggaggccgc
     1021 gcgtgctgag caagccaagt tccgcgatgc gctcgccgcg acaccgctcg atcaggtgaa
     1081 ggacgatccg gagctcctgc ggtttgcgat ggccggcggg gctacagtct tcgccgacaa
     1141 ctgcgcgccg tgccatggcc gtggtgccca gggattcccc ggctatccca atctcaatga
     1201 cgacgaatgg ctgtggggcg gtaccatcga ggacatccac accacgatcc gcttcggaat
     1261 ccgttcgggt cacgaggagg ggcgtgaaag ccaaatgccg cgatttggca tcgaccagct
     1321 tctcgaccgc aatcagattt ccgatacggc agagtacgtc ctatctttgt ccggccgggc
     1381 gacggacgag gccgcagttg cgcgcgggcg ggaaatctat gcggagcagt gcgtggcgtg
     1441 tcacggtgag gatggcaaag gcaacgtcga gctaggcgca cccaacctca cggacgcgat
     1501 ctggctctat ggcagctcga aggaagatat cgtggagagc atcaggaccg gccgtggcgg
     1561 tcagatgcca ccatgggagg gccggctcga tccagtgacg atcaagtcgc tcgccgttta
     1621 cgtccactcg cttggtggcg ggcaataaag gtgccgatga gctgttgaac gccacaggca
     1681 aatggagcgg aattgaagac cggactttgt ccgggagcaa agcgtagcgc cctcgctcat
     1741 ggtcagtagg cgggggcagg tgaggagcag tgggcggacg ggcctgctcc tcacctctga
     1801 tttcctgacg caggagggta aggagaccaa tgacctccat gccgcagaat ctgaagcctg
     1861 ccgatttcat caacgaagcc gcgcccggcg tcgagaagca cgatgttgct gccatcaacc
     1921 gcaaggatcg gcgtgagctt tatgctgctc ggcagaaggt ctatccgcgc ctcgcctacg
     1981 gccggttccg gaacatcaag tggctggtca tggcgatcac gctcagcatt tattacgtgc
     2041 tgccatggct gcgctgggac cgcggtccga atctgcccga ccaggccgta ttgctcgatt
     2101 tcgccaatgg ccggttgttc ttgttcttcc tcgagatctg gccccaggaa ctctattaca
     2161 tcacaggaat cctggtgatc tcggcccttg gcctgttcct cgtcacggcg ttggcaggtc
     2221 gcgtctggtg cggctacgcc tgcccgcaga ctgtctggac cgatctcatg atcgcggtcg
     2281 a
//
LOCUS       DASUMG010000133         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_578822, whole genome shotgun sequence.
ACCESSION   DASUMG010000133 DASUMG010000000
VERSION     DASUMG010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578822"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..672
                     /locus_tag="VFX46_02160"
     CDS             25..672
                     /locus_tag="VFX46_02160"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX5599419.1"
                     /translation="MQHLLNPRLAEELRRVPVVPVLTIDDAKLAVPLARALVAGGLRI
                     LEVTLRTQGALEVIRRIVQEVPGVVVGAGTVRTPDQGHSAISAGARFLVSPGMTPRLV
                     QSADGWSVPFLPGAATPSEAMALADLGYRALKFFPAEPLGGVATLKALAAPLPDVVFC
                     PTGGIGPETARDYLALPNVVAVGGSWVAPAAAIAKEDWDTITRLAREAAALGIKS"
     gene            complement(675..1616)
                     /locus_tag="VFX46_02165"
     CDS             complement(675..1616)
                     /locus_tag="VFX46_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEX5599420.1"
                     /translation="MTFPWKSGRAICVGECMVELARGDDQRFGLGYGGDTFNTAVYLS
                     RAGVAVAYATAVGDDPYSAGIVQTARREAVDTDLIVTVPGRLPGLYLIETTPDGERTF
                     WYWRDRAPARELFELADAERIADELRKASLVYFSGITLSLYSERGLERFAAALQAARQ
                     AGALVAMDSNYRPRGWGQNPERARSVFERFWRIADIAMPTLEDERALWGDADEQTTVS
                     RLRGLGVREVAVKLGSRGAFVAAEGVEQLVPADKGLRVVDTTAAGDSFNAGYLAARRA
                     GASSVDAARQGNRLASIVIQHRGAIVPAEATAAVLSD"
     gene            1766..>2989
                     /locus_tag="VFX46_02170"
     CDS             1766..>2989
                     /locus_tag="VFX46_02170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX5599421.1"
                     /translation="MNICMIGAGYVGLVSAACFSEFGWTVTCVDKDHARIESLKRGEV
                     PIYEPGLEDLMQRNIAAGRLSFSTDLGPAVASADLIFLAVGTPKRRGDGHADLSYIYA
                     AVSEIAPHLTGFTVITTKSTVPVGTSREIARRLRELRPDADFAVCSNPEFLREGSAIQ
                     DFTHPDRVLVGCEDPRAREIMERLYKPLALRNAPIMFVSPESAELAKYAANAFLAMKI
                     SFINEVADLCEEVGANVQEVAEAIGKDRRIGDKFLHPGPGFGGSCFPKDVSALIRIAR
                     EARSPLSLVEQVQRVNDERKISMASRIERAAGGSVRGKVIAVLGVTFKPNTDDMRDAP
                     SLVVIPMLQERGASIRAYDPQGRKYAESLLPGVEWCSNAIEAATSADIVVVLTEWNEF
                     RALNLGALREVMRGDI"
BASE COUNT          542 a          986 c          933 g          528 t
ORIGIN      
        1 cgacagagcg aacaagggtc accaatgcag catttgctca accctcgcct tgccgaagag
       61 ctgcgccgcg tgccggtcgt gcctgtcctc acaatcgacg acgccaagct ggccgtcccg
      121 ttggcgcggg cgctcgtggc aggaggcttg cgaatcctcg aggtgactct gcgtacgcaa
      181 ggcgctctgg aagtcattcg ccgcatcgtc caggaagtgc ccggtgtggt ggtcggtgcc
      241 ggaacagtgc gcacgcccga ccagggacac agcgcgatat ctgccggcgc gcggttcctc
      301 gtctcgcctg gtatgacacc gcggttggtg cagtctgccg acgggtggtc ggtccctttc
      361 cttcccggcg cggccacgcc gtcggaagcg atggcgctgg ccgatctcgg ctaccgggcg
      421 ctgaaattct tccctgccga gccactgggc ggagttgcga cgctcaaagc cttggcagct
      481 ccgctgccgg acgttgtctt ctgtccgact ggcggcatcg gaccggaaac ggcgcgcgat
      541 tacttggccc tgccaaatgt cgtggctgtg ggcggatcgt gggtggcgcc agcggcagcc
      601 attgccaaag aggattggga caccattacg agattggccc gcgaagcagc cgccctcgga
      661 ataaagagct gaggttaatc gctcagcact gccgctgttg cctcggctgg cacgatggcg
      721 ccgcgatgct ggatgacaat ggatgccagc ctgttcccct gtcgagcagc atcgacggaa
      781 gatgcaccag cgcgccgggc ggcgagatat ccggcgttga acgagtcccc ggcggctgtc
      841 gtatcgacca ctcgaaggcc cttgtcagcc ggaacgagct gctcaacgcc ttcggcggcg
      901 acgaaagcac cgcgcgagcc gagcttgacg gcgacctccc gcactccaag accgcgcaat
      961 cgagatacgg tcgtttgctc gtccgcatct ccccacagtg cccgctcgtc ctccagcgtc
     1021 ggcatcgcga tgtccgcaat acgccagaag cgctcgaaga ccgatcgcgc ccgctcggga
     1081 ttttgccccc agccgcgcgg ccggtagttg ctgtccatcg caaccagcgc gcccgcctgt
     1141 cgcgccgctt gaagagccgc cgcaaaccgc tcgagcccgc gctcgctgta aagcgacagt
     1201 gtgatgccgg agaaatacac cagcgaggct ttccggagct cgtccgcaat ccgctcggcg
     1261 tctgccagct cgaacagctc ccgtgcggga gcccggtcgc gccagtacca gaacgtccgc
     1321 tctccgtccg gcgttgtctc gatcaggtaa agacctggaa gtcgcccggg aacggtaact
     1381 atgagatcag tatcgacagc ctcgcgtctc gctgtctgaa caatccccgc gctgtaggga
     1441 tcatcaccga ccgccgtggc gtaggcgaca gctacgcctg ccctggaaag atagactgcc
     1501 gtgttgaagg tatcgccacc gtagccgagg ccgaaccgct ggtcgtcacc gcgggccagc
     1561 tcgaccatgc actcgccgac acagatcgct cgaccacttt tccaggggaa cgtcatcctt
     1621 gatccgccaa actggtgaca gaaaggacta ccttaacaga cctccatatg gccagcttga
     1681 accccttctg aatttggcat agcccggagc ggactgctgc ttcgggggcg gcagttgctg
     1741 cacagcatat tccgcgaggt cagccttgaa catctgcatg atcggtgccg gctacgtagg
     1801 actcgtctcc gcggcttgct tttcggagtt cggatggacg gtcacctgcg tcgacaagga
     1861 ccacgcgcgc atcgaaagcc tgaaacgcgg cgaggtgccg atctacgagc ctggcctcga
     1921 ggatcttatg cagcggaaca tagccgcggg gcggctgtcg ttttccaccg acctcggacc
     1981 agcggtcgcc tccgccgact tgatctttct cgccgtcggg acgcccaagc gccggggcga
     2041 tggccacgcc gatctgtcat acatctacgc cgctgtctcc gagatcgcgc cgcacctgac
     2101 aggcttcacg gtcatcacga ccaaatcgac ggtgccggtg ggcaccagcc gtgagatcgc
     2161 ccgccgcctg agggagctga gacccgacgc agacttcgcc gtatgctcca atccggagtt
     2221 cctgcgcgaa ggctccgcca ttcaggactt cacacatccc gaccgcgtgc tcgtcggctg
     2281 tgaggacccg cgcgcacgcg agatcatgga acgcctttac aagccgcttg ccttgcgcaa
     2341 cgcgccgatc atgttcgtca gtcccgagtc ggcggagctt gccaagtacg ccgccaacgc
     2401 cttcctcgcc atgaagatca gcttcatcaa cgaggtggcg gatctgtgcg aggaagtcgg
     2461 cgcgaacgtg caggaagttg cggaggccat cggcaaggac cgtcgcatcg gtgacaagtt
     2521 cctgcaccct ggccccggtt tcggtggttc ctgtttcccc aaagacgtgt cagccctgat
     2581 ccgcatcgcc cgcgaggcgc gctcgccgct gtcgctcgtc gagcaggttc agcgggtcaa
     2641 cgacgagcgt aagatttcca tggcgagccg gatcgagaga gccgccggcg gatccgtacg
     2701 tggaaaggtg atcgcggtcc ttggcgtgac gttcaagccg aacaccgacg acatgcgtga
     2761 tgcgccgagc ctggtggtca tccccatgct ccaggagcgc ggggcaagca tccgggctta
     2821 cgatcctcaa ggccgcaagt acgccgaaag cctgctgccg ggggtcgagt ggtgcagcaa
     2881 cgcgatagag gcagccacgt cggccgatat cgtcgtagtc ctgacggaat ggaacgaatt
     2941 ccgtgcgctc aacctcggcg ccctcaggga ggtcatgcgc ggggacatc
//
LOCUS       DASUMG010000134         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_338786, whole genome shotgun sequence.
ACCESSION   DASUMG010000134 DASUMG010000000
VERSION     DASUMG010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338786"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..644)
                     /locus_tag="VFX46_02175"
     CDS             complement(84..644)
                     /locus_tag="VFX46_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599422.1"
                     /translation="MSTATEAFAGQLVENIPRRMTAHVPELVEVSIAKAEVKALAERL
                     QGSGTAYRHDVVVTKAMSVRLRAPEGGFWIEMASPETQWIENTLGLLSDDYASWRWIV
                     TPQRRGKARLQLIVSARTVGADGLAAETALPDQVVEVKIRTNYHKTAVQWGGWIAAAG
                     LGAILARFGEGVWEIGSALLTQIGLI"
BASE COUNT          358 a          671 c          613 g          378 t
ORIGIN      
        1 ggccaagggc atagggatcg acgtcttgcg cgcaacgatt gccgagctgt gtaagacgcc
       61 cggagccgcc tgagatcgac gtgtcagatc aaaccaatct gcgttagcag cgcactgccg
      121 atctcccaaa ccccctcgcc gaaccgcgca agaatcgcgc ccaacccagc tgcagcaatc
      181 caaccacccc actgcactgc cgtcttgtgg taattggtcc ggatcttcac ctcgacaact
      241 tggtctggca gcgcggtctc cgcagccagc ccatccgcgc cgacggtgcg agcagaaacg
      301 ataagttgta gccgcgcttt gccccggcgc tgtggcgtca cgatccagcg ccagcttgca
      361 taatcgtcgg aaagtaggcc caaggtgttc tcgatccact gagtctccgg cgaagccatt
      421 tcaatccaga aaccgccctc aggtgcgcgc aaccgcacag acatcgcctt tgtcacgacg
      481 acatcgtgtc gatatgcggt acctgaaccc tgaagccgct cggcaagagc cttcacctcc
      541 gctttggcaa tgctgacctc gacaagctcc ggcacatggg cggtcatccg gcgtgggatg
      601 ttctctacca gctgaccggc aaaggcttcc gtggcggtag acacctcgtg acccggcgtc
      661 gcctttggac gcgcaccgtg ttgcgacgac cgggatccgg acaaaggcgg ttccccccga
      721 gcgaatggtg gtggcgtcgg ctcagaccaa ggcgactgct ctcttggata atgaggcggc
      781 ggcggaggat gtgcttgcac cgcgccctgc acgggctgcc caggcatccg aggcgggggc
      841 ggcggtcgtt tcgcatcagg ttcgcgcaag acttgcggcc gatagtatcc atccatccga
      901 gaatggctcg gccagtcgag tgtcggagca ggcggcccaa cgtgattgga acctccgggt
      961 gccggttcgg ggctgacgaa atcgcccgac tcacctcggt gatgatgctg tgctccgtgc
     1021 ggaatggacg ggcccatcgc cggcgcatca tgaccatgct taccgatttc cgagcggagt
     1081 acctcgggag gcaagctcgc gggccccggc tcagggcgcg ggccatgctg tacttcggcc
     1141 ctcggacgag cagaggtggg ctccggctgc tcaggagaat agcgcccgtc aggcggctgc
     1201 ccatgcgctc gaccgtggtc ctccgcgtga acggaatcct ctacgggcgg actcgcctca
     1261 ggcctacgtg tgccattgat cccagtcgca acgcgccgcc gtgccgccgc aagaatatcc
     1321 tcagtcgtta ggccagatgg gccattccgt tggttgtggc cgagatatgg ctcttgtgag
     1381 accggcctcc cggcctcggg ccccccagat tgagcttccg gagctgcttg atgtccaggc
     1441 tctggcgctg attgtgtcgg cggctgccct gcgctgcgcg ccgttgcgtt gaccttctgc
     1501 agagccttga ttgcctcctc gaacgtcagt ccctgatcgc gcagcatgct tgctgccgcg
     1561 cttcggccgt cgccaacaat tgctgccaag acgatggcgc cgttcacctc gcggcgccga
     1621 ctctgcctcg ccgcggccgc ggcatactcc acgatacgaa tcagctcggg ggacgctatc
     1681 gggtccgatg actcgcccgg cgacgagcgc tcaaccgatt ctgcaacgta gctcgaggca
     1741 tcgccactta gtctatcaag gtcgacatgg caggcctgca gcaccgtcga cgcgtctttg
     1801 tccgcaatca gagccaggag caaatgctcc aaagtgactg tgcgatgcga ctggctcttg
     1861 gcgtagtcga aagcgcgcgc gagcgttgtc gccaggtcgc gtgacttcgg cacccagctc
     1921 aatgcatcgg cgtacggctg cgacatccaa gctccccttt cggctcctac cactgtgaaa
     1981 aaggagccgg ccccaccctc attgtcaggc gaatctgctc
//
LOCUS       DASUMG010000135         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_499479, whole genome shotgun sequence.
ACCESSION   DASUMG010000135 DASUMG010000000
VERSION     DASUMG010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499479"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFX46_02180"
     CDS             <1..582
                     /locus_tag="VFX46_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DjiA"
                     /protein_id="HEX5599423.1"
                     /translation="SVAFTIAVISLCAKLSKADGVSTRLEAEAFEEIFHVPPEERANV
                     ERLYDIAKQDVAGYDAYADKISRLLADEPDLKRNVLEALFHIAAADGIFHGAEDAYLA
                     DVARRFGFSDTEYRAIRRLFVREPNDPYTVLGVEPDISNNELKAHYKRLVREHHPDTL
                     MARGVPKEFIDVANRKLAALNAAYDEIAKERGL"
     gene            579..1349
                     /locus_tag="VFX46_02185"
     CDS             579..1349
                     /locus_tag="VFX46_02185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5599424.1"
                     /translation="MTFEPDSPLVSEVCPSPNFEPRRENRQPTMLLLHYTGVPTCRGA
                     IDWLARPESKVSAHYVIDEAGRIVQMVRESMRAWHAGLSRWGSEVDINSASIGIEIHN
                     PGHDGGYPDFTDAQMEAVAALCRDIIVRHGIRSERVLAHSDVAPVRKRDPGEKFDWAR
                     LAKAGVGHWVEPEPVARSDRGIGLGYGGMLVAEVQEMLARYGYDIEPTGDFEEKTMFV
                     VTAFQRHFRPARVDGRIDQSTITTLERLLAALPENQAA"
     gene            1359..2276
                     /locus_tag="VFX46_02190"
     CDS             1359..2276
                     /locus_tag="VFX46_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5599425.1"
                     /translation="MQKNVPSSAPIDGLAVFLVLACCASWGMNQVAIKVANTGIPPML
                     QAGLRSVVSGLLLLGWARARGIPLFNRDGSLWAGIAAGVAFGLEFLLLYQGLEWTTAS
                     RGVLFLYSMPFFVAIGAHLLIPGEKLTVIKAVGLVGAFCGLAIALGEGLTGEAGEGDI
                     VGDIFCIIAGFAWAATTILIRTTTLRFISAEKVLFYQLAVSAPLMIVASLIAGEGAAT
                     FSDPRVVLAFAYTAVVVAFISYVAWFWLLARYSPSAMSVFTFLTPIFGAFAGVLVLGE
                     AMTWRLLVALALVALGIYLVNRPSGPRVA"
     gene            2317..2739
                     /gene="rnpB"
                     /locus_tag="VFX46_02195"
     ncRNA           2317..2739
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFX46_02195"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT          489 a          879 c          933 g          544 t
ORIGIN      
        1 agcgtggcgt tcaccatcgc cgtgatctcg ctttgtgcaa agctgtcgaa ggcggatggc
       61 gtctcgacgc gcttggaggc ggaggccttc gaggagatct tccacgtgcc gccggaggag
      121 cgcgccaatg tggagcgcct ctacgacatc gccaagcagg atgtcgccgg ttatgatgct
      181 tatgccgata agatttcgcg gctgctcgcc gatgagccag atctgaagcg gaacgttctt
      241 gaggctttgt tccacatcgc tgccgcggac ggcatcttcc acggagccga ggatgcgtat
      301 ctggccgacg tggcgcggcg tttcggcttc tcggatacgg agtatcgcgc catccgccgg
      361 ctgttcgttc gtgagccgaa cgacccctat actgttctcg gcgttgagcc ggacatttcg
      421 aacaatgaat taaaggccca ttacaagcgg ctcgtgcgcg agcaccatcc cgatacgctg
      481 atggcgcgtg gcgtgccgaa ggagttcatc gacgtggcca accgcaagct cgcggccctc
      541 aatgccgcct atgacgagat tgcaaaggag cgtggtctgt gacgttcgag cccgatagtc
      601 cgctggtcag cgaggtttgc ccgtcaccga acttcgagcc gcgccgcgag aaccgccaac
      661 cgaccatgct cctcctccac tacacaggcg tgccgacctg ccgtggtgcg atcgattggc
      721 tcgcgcgacc cgaaagcaag gtgtcggccc actatgtcat cgacgaagcc ggccgcatcg
      781 ttcagatggt gcgcgagtcg atgcgggcgt ggcatgcagg cctgtcgcgc tggggaagcg
      841 aggtcgatat caattccgct tccataggca tcgagatcca taaccctggc cacgacggcg
      901 gctatcccga tttcacggat gcacaaatgg aagcggtggc ggcgctgtgc cgggacatca
      961 tcgtccgcca cggcattcgc tcggagcgcg tgctcgccca ttcggacgta gcgcccgtac
     1021 ggaagcggga tcccggggag aagttcgact gggcccggct cgccaaagca ggcgttggcc
     1081 attgggtcga gccagagccc gtcgcccgct cggatcgtgg gatcggcctc ggctacggtg
     1141 gaatgctcgt tgccgaggtg caggagatgc tggcgcgcta cggctacgac atcgagccga
     1201 cgggcgactt cgaggagaag accatgttcg tggtgactgc cttccagcgc cacttccgcc
     1261 cggcgcgggt cgacggccgt attgaccaat ccacgatcac gaccttggaa cggttacttg
     1321 ccgcattgcc ggagaaccag gccgcctgag ggaaactcat gcagaagaat gtgcccagct
     1381 cggccccgat cgacgggctg gccgtattcc tggtgctggc atgctgtgcg tcctggggta
     1441 tgaaccaggt ggcgatcaag gtcgctaaca ccggcattcc tcccatgctc caggcagggc
     1501 tgagatcggt cgtgtccggc cttctgctgc tgggctgggc ccgagcgcgt ggcattcccc
     1561 tctttaatcg cgacggaagc ctctgggccg gcattgcggc gggcgtggcg ttcgggctgg
     1621 aattcctgtt gctttaccag gggctcgagt ggacgacggc ctcgcgcggg gtgctgttcc
     1681 tctattcgat gccgttcttc gtcgccatcg gcgcgcatct gctgatcccg ggagaaaagc
     1741 tcacggtaat caaggcagtg ggcctggtgg gagccttttg cggcctcgcc atcgcactcg
     1801 gagaagggct gaccggagag gccggggagg gcgacatcgt cggcgacatc ttctgcatca
     1861 tcgcgggctt cgcgtgggcg gcgacgacca tattgatccg cacgaccacg ctacggttca
     1921 tctccgccga aaaagttctc ttctaccaac tcgctgtctc ggctccattg atgatcgtcg
     1981 cctcgctcat cgcgggggag ggggcggcga cgttctccga ccctcgcgtg gtgctcgcct
     2041 tcgcctatac cgccgtcgtg gtcgcgttca tcagctatgt ggcctggttc tggctgttgg
     2101 cgcgctactc gccgtcggct atgtccgtct tcacgttcct gacgccgatc ttcggagcat
     2161 tcgctggcgt gctggtttta ggcgaggcga tgacgtggcg ccttctggtg gcgctcgccc
     2221 tcgtcgcgct cggcatctat ctcgtcaaca ggccctctgg tccaagagtg gcgtgaaggg
     2281 ttgacccgga cgttgaacgc tcccttattc actatgccag ccggtcggac ggccgctgcc
     2341 agcgctctcg ctttctggcg acaaggcgct gggagaggaa agtccgggct ccatggaaac
     2401 acggtggcgg ctaacggccg ccgggggcga ccccagggaa agtgccacag aaagcaaacc
     2461 gccgacagcg gctcgccgag ccgctgggca agggtgaaag ggtgcggtaa gagcgcaccg
     2521 cgcgactggc aacaggagcg gcagggcaaa ccccaccggg agcaagacca aataggggtg
     2581 gccaggcgag cgggagtgtc cccgtggcgc caagcccgtt tccaggctcg ccacccgggt
     2641 tggttgcacg aggcgcgcgg caacgcgcgt cctagatgaa tggccgtcgc ggagaggcaa
     2701 ctctccagac agaacccggc ttacaggtca actggcactt gatcttccgg cgcaccgacg
     2761 ctcgccgcaa agtcttgctt aatcgaatct caaccgcgct tacgcaaagt gcggatgtct
     2821 tttgcgtacc ggccgagcgc cgggt
//
LOCUS       DASUMG010000136         2687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_178382, whole genome shotgun sequence.
ACCESSION   DASUMG010000136 DASUMG010000000
VERSION     DASUMG010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178382"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VFX46_02200"
     CDS             complement(<1..390)
                     /locus_tag="VFX46_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599426.1"
                     /translation="MRGTQLAPEARIKLKIGSRPGFVELDINRGGISGLKPAHRLGRD
                     FLTYVLWAVSIDGKASNLGEITFEGEHPVPVNLTTPYQTFWLMVTAEPNYAVVDPSSH
                     VVLYSVKEGSDSETRALPIRSNLFYFTH"
     gene            complement(700..867)
                     /locus_tag="VFX46_02205"
     CDS             complement(700..867)
                     /locus_tag="VFX46_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599427.1"
                     /translation="MTNHTAVISDLDQDIRWRDWQARGVASDRRTSARMRKLMLLIVA
                     ALVVWFVVQLT"
     gene            complement(1047..>2687)
                     /locus_tag="VFX46_02210"
     CDS             complement(1047..>2687)
                     /locus_tag="VFX46_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5599428.1"
                     /translation="VPGILIAVSLVFMVLGLAGGGVARQINNLADDLPRYRVNILAKI
                     ADVRGAGKGGSVEKLQETIEGIKTELGQSSARTGTASRPVVVSSELVPGFSGFSWLGP
                     LLGPLGTAGLVLAMVIFMLLERRDLRDRLIGLFGHGQLTVTTKAFDEAGTRVSRQLLM
                     QSMVNLVYGIVAGLGLYFLGVPHPMVWAALGAALRFIPYIGPVIGAGAPILVSLAALP
                     GWAGPLGVVGLFVGLELFTNLVLETVLYAGAAGVSQVALLVSLAFWTWLWGPLGLLMA
                     TPLTVCLVVLGKHVPGLEFVGMLMADTPALAPEYGYYQRLLARDQSEAADLIERHIKT
                     EAPRSVYDALLLPALNYAERDRLEQRLSLDEETAVIDATRELLSDAAESIRRIEPEPS
                     PPDDPTPSAPREPLRVLGFAANGSADELGLEMLGHVLNDLPILVEVTRRRLLASELVS
                     LVQEQEVSVICLADLPPSPPSKTRYLVKKLHAALPNVRILVGRWGPEALADDSTQLLR
                     DAGATLVAATLAETRTYLSGLLQVPTMPLEGAGSDQAA"
BASE COUNT          512 a          863 c          855 g          457 t
ORIGIN      
        1 gtgggtgaaa taaaagaggt tgctgcgaat cggaagcgct ctcgtctcgc tgtcagatcc
       61 ttccttcacg ctgtagagga ccacgtgaga actcggatcg accaccgcgt aattcggttc
      121 cgcggtcacc atcagccaga acgtctgata gggcgtcgtc agattgacgg ggactggatg
      181 ctcgccctca aaggtgatct cgccgagatt ggaggccttg ccgtcgattg aaaccgccca
      241 cagcacgtag gtgagaaagt ctctgcccag ccggtgcgcg ggcttcaagc ccgagatgcc
      301 gccgcgattg atatccagtt cgacaaagcc ggggcggctg ccgatcttca gcttgatccg
      361 cgcctccggg gccagctggg tgccacgcat atacacctcg atcgagcccg cgaggtggcg
      421 atagctcagg atctggccgt tcggctcctt cagttcaacg gccctgagct gcgccgccct
      481 cggctccggc gtctggggct cggcgacctt gggcttgggc cctgacgccg acgcagtgat
      541 cgacaacacg ctcagaaaag cggccagctt cagcagtgtc gatacgctcg aacgtggcac
      601 tcgatgggtt atcggcggcc cggcagcggt tgtccagcgt ccagggaact tgcgatgcct
      661 gttgggacac gcccggctcg ggtggtgctc actctttcat cacgtgagtt gaacgacgaa
      721 ccagacgacc aaagcggcga cgatgagcag catcagtttg cgcattctcg cacttgttcg
      781 ccggtcactg gcgacaccgc gcgcctgcca atcgcgccac cggatgtcct ggtcgaggtc
      841 ggaaatgaca gctgtgtgat tcgtcatcga cgtgtctcct gaatggtctc ggacatggtt
      901 gcgcgaggag cattccaggg ccacatcatg acgagcggcg ggagaaccgc gaggctcgcg
      961 agaaccgtta cctattgaaa gtgtacgggc cggcacctcg cggcgctgtt cccaagcgca
     1021 caccgctcgt gccgtcgatg ccgtcgctaa gccgcctggt cgctgcctgc cccctcgagc
     1081 ggcatagttg gaacctggag cagcccgctc aggtaagtcc gggtctccgc gagtgtcgcg
     1141 gccacgagag tcgcgccagc atcgcgcagc agctgcgtgc tgtcgtcggc cagcgcctcc
     1201 ggaccccagc gcccgacgag gattcgcaca ttcggcaaag cggcgtggag cttcttgacg
     1261 agatagcggg tcttggatgg agggctgggc ggtagatcag cgaggcagat gacagaaact
     1321 tcctgctctt gtaccagcga caccagctcc gaggcgagca agcgtcgtct ggttacctcc
     1381 acgaggattg gcagatcgtt cagcacgtgg ccgagcatct ccagcccaag ctcgtcagcg
     1441 ctgccattgg ccgcgaagcc cagcacacgc agcggttctc gtggggcgga aggcgttggg
     1501 tcatcaggcg gtgacggctc cggctcgatg cgccgaatcg actccgccgc gtcggacagg
     1561 agttctcgcg tcgcatcgat gaccgccgtc tcttcgtcga gcgacagccg ttgctcgagg
     1621 cggtcgcgct ccgcatagtt gagtgccggc agcagcaagg cgtcgtagac cgagcgcgga
     1681 gcctccgtct tgatgtgtcg ctctatgagg tcggccgcct cgctttgatc gcgggccagc
     1741 aagcgctgat agtagccata ctctggggcg agcgcggggg tgtcggccat cagcattccg
     1801 acgaactcga gacctggtac gtgtttcccg agtacgacga gacacacggt cagcggtgtc
     1861 gccatgagca ggccgagcgg gccccacaac catgtccaga aggccaacga cacgagcagc
     1921 gccacctggg agacgcccgc cgcacctgcg tacaaaacgg tctcgagcac gagattcgtg
     1981 aacagctcga gcccgacgaa cagacccacc accccgagcg gaccggccca gcctggtagc
     2041 gcggcaaggc tcaccaggat cggcgcaccg gcaccgatca cgggtccgat gtaggggatg
     2101 aaccgaagtg ccgcaccgag cgccgcccag accatcgggt gcggcacgcc gaggaagtag
     2161 aggccgagac ccgcgacgat gccgtagacc agattgacca tggactgcat cagcagctgc
     2221 cgactgacac gggttccggc ctcgtcgaat gcctttgtcg taactgtcag ctggccgtgg
     2281 ccgaacagcc caatcaaccg atcgcgcaga tcccggcgtt cgagcagcat gaagatcacc
     2341 atcgccagca cgagcccggc ggtccccaac ggaccgagca acggaccaag ccacgagaag
     2401 cccgagaaac ccgggaccag ttcggagctg accacaacgg gccgcgaggc cgttcctgtt
     2461 cgcgcgctcg attgtccaag ctctgtcttg atcccttcga tggtctcctg aagcttctcg
     2521 actgagcctc cctttcctgc tccgcggacg tcggcaatct tcgccaggat gttcacgcgg
     2581 tagcggggga gatcgtcggc gagattgttg atctgccgcg ccacaccccc gccggcaaga
     2641 ccgagcacca tgaacaccag gctgacggcg atgaggatgc cggggac
//
LOCUS       DASUMG010000137         4853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_178400, whole genome shotgun sequence.
ACCESSION   DASUMG010000137 DASUMG010000000
VERSION     DASUMG010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178400"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFX46_02215"
     CDS             <1..272
                     /locus_tag="VFX46_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5599429.1"
                     /translation="LLLTELPKAGFDRIVPADGAFYLYADVSRFTDDSLAFAKLMLDE
                     TGIAATPGVDFDETRGRRFIRFSYAGTTADMAEAVERLQKWSRLK"
     gene            390..1109
                     /locus_tag="VFX46_02220"
     CDS             390..1109
                     /locus_tag="VFX46_02220"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5599430.1"
                     /translation="MMMLRCIGVLLLSMLLADAGYAQALKQKTVTFEAKGKRLSATLR
                     IPHVTNPLPAIIIGHGTAGIDERGEFYRIPLEAAGFATLEVDFKSGIFTSPKDRPRNP
                     AFIPYLFAAVAALHEQAEIDHSRIGVLGFSLGGQLALNAAMRDLSAPYTSKGLKIAAA
                     VSLYPGCQLLVKRANAFNGMPTLILVGSNDHYTTPDDCYSAERLANARYPGSVDVVVY
                     EGAFHAFDRPRSKANDPRSER"
     gene            complement(1209..2204)
                     /locus_tag="VFX46_02225"
     CDS             complement(1209..2204)
                     /locus_tag="VFX46_02225"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5599431.1"
                     /translation="MDIQDLRIFARVAAVQNLSSVGTELGLTPGTISKRIQALESELR
                     VRLFDRTTRSIRITEEGATFLSYVERILDELESARAAVGENATRPKGKLKVSAPATFG
                     RFIVAPAICAFMRAYPEIEVQIDLTDRNVNLQEEGYDVAIRTGALTDSVLIAKRLCAD
                     RQVVVASPAYLEAHGVPRTPADLARHNCLVLKNNWQWSFSRGKEDFSIRVNGRLMSNN
                     GEILLHAALDGLGLLQTSELRVREDLAAGRLVRVLEEYEIASNSAVWAVYPSSKHVLP
                     KLRALLDFLAGWFREVEAQLPRTANRIGSGRKSEVSFPLPKPSTRQCQQPNALLP"
     gene            complement(2428..>4853)
                     /locus_tag="VFX46_02230"
     CDS             complement(2428..>4853)
                     /locus_tag="VFX46_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HEX5599432.1"
                     /translation="EIVLGAFGVALLAALIAVARVRAPTRDEAIRRIERRSGIPHRPA
                     SSYEDTLTIPSGNSATERIWQAHKARLAALLDRLRVGAPQPRTDRKDPFALRALLILS
                     VVLLMGLVGDSAKDRLFAAFRFSPEVQLANARLDAWVTPPAYTGKPPILLADGGAGTL
                     RTAAEGTDLIEVPDKSLLIIRASGAGQLSLEIAATGAEEPQTVEGQAPKQAGDVSEVR
                     AELRNSGTVRVLGSGSELTAWNFRVIPDQAPKIVLTKEPESTIRGGIKLFYKVEDDYG
                     VASAEARFARPRTEAGDPRTRWARADLLKGPRPPLERPPVLNLRLPRQNTENSETSSF
                     HQLGAHPYAGMKVVMTLVAKDHAGNVGKSQPIEIILPQRRFLKPLARAVIEQRRKLVE
                     DPRYRPLVVSALDALTYEPEGFIEDKHVYLGLRSVYHRLSRSGSRESLRSAVEQLWHI
                     ALRIEDGGALTDAERRLRDIQDRLSKALEEGASDEEIARLMQELRQAMAEFLQELARQ
                     AQGQEMQLPEGFNPNQIMRPQDLQRMLDQLENMARQGSREMAQQLLDQLRELMESLQA
                     GRMMGRQGQQGQQMMQMMDQMGDIIGKQQQLYDDTFGEQQRQQGERGQQGQRGQQGQQ
                     FGRRGQGQRGQQGQRGQQGQGQQGRSFDKGELADRQGQLRDRLGQLRQGLQNFGMQTP
                     GQLDGAEQSMENARRALEQGDLDRATREQSRALEQLRQGAQSMAEQMLRQMPSRFGQG
                     PTGDVPRDPLGRPQRSEGPDLGTSVKVPDEIDTQRAREILEELRRRLGEPTRPMLELE
                     YLERLLRRF"
BASE COUNT          934 a         1457 c         1458 g         1004 t
ORIGIN      
        1 agctccttct gaccgaactg ccgaaagccg gctttgaccg catcgttcct gccgacggag
       61 ccttctatct atatgcggac gtcagccggt ttacggacga cagcctcgcc ttcgcaaagc
      121 tgatgctcga tgaaaccggc attgctgcaa cgcctggagt cgacttcgac gagactcgcg
      181 ggcgccgctt catccgtttc tcttatgccg gcaccactgc cgacatggcc gaagccgtcg
      241 aacggctgca aaaatggagc cggctcaaat agaagaaagg tcgatttcgg aaagagataa
      301 ctgcgacctg gggccgccta cgatacagac tgacaagggt agctggttcc tttaaattcg
      361 gcggtcgtct gaagattatt tcagagttta tgatgatgct tcgttgcata ggcgttctgc
      421 tgctgagcat gttgcttgct gatgctggct acgctcaagc attgaaacag aaaactgtaa
      481 cctttgaggc aaaaggaaag aggctttcgg ccactttgcg gatcccgcac gtgaccaacc
      541 cgctaccggc gatcatcata ggccacggaa ctgccgggat cgatgagcga ggtgaattct
      601 acagaattcc actggaggcc gctggttttg caacgctgga agtggatttc aaatcgggta
      661 ttttcaccag tccgaaggac agaccaagaa atccggcttt catcccctat ctctttgcag
      721 ccgtagccgc gctgcacgaa caggcggaaa tcgatcatag tcggatcggt gttttggggt
      781 tctcgttggg cgggcagttg gccctcaatg ccgcaatgag ggatttgtcc gcaccctaca
      841 catcgaaagg tctgaagatc gcagcggcag tgtcgctgta ccctggatgt cagcttctgg
      901 tgaagcgtgc taatgccttc aacggcatgc ccacactcat tctggtaggc agcaacgacc
      961 actatacgac tccggacgat tgctattctg ccgaacgcct ggcaaacgcc cgttatcccg
     1021 gtagcgtaga tgtcgtcgtc tacgaaggcg catttcatgc attcgatcgg cccaggtcga
     1081 aggcaaacga tccaagatcc gaacgctagg caaggcaaag cggtggtagc ctatgacaag
     1141 gcagccgccg aagacgcccg gaggcgcgtt gtagagtttt tcaaggcgag attggcacta
     1201 gctggaggtt agggcaacaa ggcgttgggc tgctggcatt gacgcgtgct tggcttcggc
     1261 agcggaaacg aaacttcgct ttttcgtccg ctgccgatgc gatttgctgt ccgtgggagt
     1321 tgcgcttcga cctcgcggaa ccaacccgcc agaaaatcga gtaaagctct caatttcgga
     1381 agcacgtgct tggagctggg gtaaacagcc caaaccgccg agttgctggc aatctcgtat
     1441 tcctcgagca ctcggacgag tctcccggct gccaagtctt cccggacgcg gagctccgac
     1501 gtctgcaaca agccgaggcc gtcaagggcg gcatggagaa gaatctcgcc attgttcgac
     1561 atcagcctgc cgttcactct gatcgagaag tcctccttgc cccgactgaa cgaccactgc
     1621 caattgtttt tcaaaacgag acagttgtgg cgggcgaggt ctgcaggcgt ccggggcacg
     1681 ccgtgggctt caagataagc tggcgaggcc accacgacct gtctgtcggc acagagccgc
     1741 ttggcaatca gaaccgaatc cgtcagcgcg ccggtgcgga ttgcaacgtc gtagccctct
     1801 tcttgaagat tgacgttgcg gtccgtcagg tcgatctgaa cctcgatttc cgggtaggcg
     1861 cgcatgaatg cgcatatcgc tggagctacg atgaagcgcc cgaaggtggc gggggcagag
     1921 accttcagct tacctttggg gcgtgtcgcg ttttctccaa ccgcggcgcg cgcactctca
     1981 agctcatcaa gtatacgttc gacataggac aggaaggttg caccttcctc ggtgattcgg
     2041 atcgaacgag tcgtacggtc gaagagccgt acgcgaagct cgctttccag cgcctggatt
     2101 cgcttggaga tggtacctgg cgttaagcca agctcagttc cgaccgacga taaattctgt
     2161 actgcggcga cgcgcgcgaa gatccgcaga tcttgaatat ccatgatcac cctcagctac
     2221 gcggccattg ggagcgtagg tcggcgcagc tctcctaagg cgattgtcag caagttaaag
     2281 gtttatgcgc taaatgattc aacctgcaag tcttgtctgg cgataggagc cctgcttagc
     2341 ccgaatagcc acaggtattg acatgggccg tgttccacgg gcaccggccg ctcaaaggtc
     2401 cgccgaagga gcaaatcgct gatgtcttca gaatctgcgt agcaggcgct cgagatattc
     2461 gagctccagc attggccggg tgggctcgcc tagacgacgg cgaagctcct cgagaatttc
     2521 tcgagcccgc tgagtgtcga tctcgtccgg caccttgacc gaagtgccaa gatcaggccc
     2581 ttcggaacgt tgcgggcgcc ctaaaggatc gcgcggtaca tcccccgttg gtccctgtcc
     2641 gaaacgggag ggcatctgac ggagcatctg ctcggccatg gattgggcgc cctgacgaag
     2701 ttgttcgagg gcccgcgatt gttcgcgcgt ggcgcggtcg agatcacctt gttccagggc
     2761 acggcgggca ttctccatgg actgctcggc accgtcgagc tgacctggtg tctgcatgcc
     2821 gaagttctgc aatccttgcc gtagctggcc gaggcgatcc ctgagctgcc cctggcggtc
     2881 ggcaagctcg cccttatcaa aactgcggcc ctgctggccc tgaccttgct gaccacgctg
     2941 cccttgctgg ccacgctgcc cttgaccgcg tcggccgaac tgctgacctt gctgaccgcg
     3001 ctggccctgt tgaccgcgtt ctccttgctg gcgctgttgc tctccgaacg tgtcatcgta
     3061 gagctgctgc tgcttgccga tgatgtcccc catctggtcc atcatctgca tcatctgctg
     3121 accttgctgg ccttgccggc ccatcatgcg gccggcctgc agcgactcca tcagctctcg
     3181 aagctgatcg agcagctgct gcgccatttc gcgcgagcct tggcgggcca tgttctccag
     3241 ctggtcaagc atccgctgaa gatcctgagg acgcatgatc tgattcggat tgaagccctc
     3301 aggcagctgc atctcttggc cctgagcttg tcgcgcgagc tcctgcagga actcagccat
     3361 ggcctgccgc agctcttgca tgagccgcgc gatctcctcg tcggaggcac cttcctcgag
     3421 agctttcgag agccgatcct ggatgtcccg aaggcggcgt tcggcgtcgg tcaatgctcc
     3481 gccgtcctcg attcgcagcg cgatgtgcca gagctgttca acggcactgc gtagcgattc
     3541 gcgcgaaccg ctccgtgaaa gtcgatgata gaccgacctc agcccgagat agacgtgctt
     3601 gtcctcgatg aacccttcgg gctcatacgt caaagcgtcc agggcgctga ccaccagcgg
     3661 ccgataccgc ggatcttcga ccagcttccg gcgctgctcg atcaccgcgc gggcgagggg
     3721 cttaaggaac cgccgctggg gcaggatgat ctcgatcggc tggctcttac ccacgttgcc
     3781 ggcatgatcc ttggcgacaa gggtcatgac aaccttcatg ccggcataag gatgggcacc
     3841 aagctgatga aagctcgatg tctctgagtt ctcggtgttc tgtcgcggca ggcgtaggtt
     3901 gaggacgggc ggccgctcca atggcggtcg cggccccttc agaagatcag cccgcgccca
     3961 gcgcgtccga gggtcaccgg cttccgtgcg gggacgtgcg aagcgtgctt ccgcagaggc
     4021 cacgccgtag tcgtcctcga ccttgtaaaa cagcttgatg ccaccccgga tcgtgctctc
     4081 cggctcttta gtgagcacga ttttcggtgc ctggtccggg atgacccgga aattccaggc
     4141 agtcaactcg ctgccgctgc cgagcacgcg gacggtgcca gaattgcgca gctcggcgcg
     4201 gacctcggat acgtcgccag cctgctttgg cgcctgacct tcgaccgttt gcggttcctc
     4261 cgccccggta gcagcaatct ccagggacag ttgtccggca ccgctcgccc ggatgatcaa
     4321 gaggcttttg tctggtacct cgatgagatc ggttccctca gcagcggtcc gcagtgtgcc
     4381 ggcgccaccg tcggcgagta gaatgggcgg ctttccagtg tatgccggtg gcgtaaccca
     4441 agcgtcgaga cgtgcattcg caagctggac ttccggacta aagcggaaag cggcgaacag
     4501 cctgtccttg gcgctgtcac ccaccaagcc catcagcagc acgacgctga ggatcagaag
     4561 tgcccgaagg gcgaacgggt ctttgcgatc ggtccgaggc tggggtgctc cgaccctgag
     4621 ccgatcgagc agggccgcca gccgagcctt gtgtgcctgc cagatgcgct ccgtagcgga
     4681 gttgccgctc ggaatcgtca gcgtgtcctc gtaggaggag gcgggccggt gagggatgcc
     4741 cgaccggcgc tcgatgcgtc gaatggcctc atcacgagtt ggagcacgca ccctggcaac
     4801 cgcgatcagc gcagcgagca gggcaacgcc gaacgcgccg agcacgatct cgt
//
LOCUS       DASUMG010000138         2187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_17811, whole genome shotgun sequence.
ACCESSION   DASUMG010000138 DASUMG010000000
VERSION     DASUMG010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17811"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="VFX46_02235"
     CDS             <1..921
                     /locus_tag="VFX46_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5599433.1"
                     /translation="VNPDERLKDKTFEDYMFEGALAATNAVMRETGEDQINVLGYCVG
                     GTLLATLLAYLAARGEDLYKSATFLAAQVDFNHAGDLLVFIDDAQLAALEEMMAERGY
                     LDGSRMATVFNLLRPKDLIWPYVVNNYLLGKKPFPFDLLYWNQDSTRMPAANHRFYLR
                     EFYHENKLAKGEMKIAGTRLDLKQVKLPIYELATREDHIAPAKSVFVGSKLFGGPVEF
                     VLAGSGHIAGVINPPDPNRIKYQYWTNKKPAETLEEWIAGAKETPGSWWPHWTKWLAK
                     HSGKRVPAREPGAVLGVIEDAPGSYVKVKG"
     gene            complement(982..1764)
                     /locus_tag="VFX46_02240"
     CDS             complement(982..1764)
                     /locus_tag="VFX46_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599434.1"
                     /translation="MDLGLKGRRALVLASTKGLGRAVATALAGEGTSVMICGRFGAAQ
                     AAAEISRETSGAVMGRDCDLHDRAAIDDLFQAAVGEMGGVDILVLNSGGPPPAPALEV
                     SDKAWSEWFERMVGNLIHAATLALPAMRERKWGRLLTIASSAAVQPIPGMALSNSLRA
                     SLLAWNKTLAAEVAADGITCNVVLPGRIDTERVAALDEARAKREGRSVEEIRRDVLAG
                     IPIGRYGRPEEFGSVAAFLCSDRASYVTGTVVRIDGGLIRGI"
     gene            complement(1774..>2187)
                     /locus_tag="VFX46_02245"
     CDS             complement(1774..>2187)
                     /locus_tag="VFX46_02245"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5599435.1"
                     /translation="RPRTTLTPSMALRDGKPYLAWGTPGGDQQDQWSLILFLRHVHHG
                     LNLQEAIDAPSFHSEHCPSSFWPREARPNRLVLEGRFPPETVAELKARGHDVVVGEDW
                     SEGRLSACSREDRGGTLYLRAAANPRGMQGYAVGR"
BASE COUNT          429 a          696 c          682 g          380 t
ORIGIN      
        1 gtcaaccctg acgagcggct gaaggacaag acgttcgagg actacatgtt cgaaggcgcg
       61 ctcgcagcca ccaatgctgt catgcgcgaa accggcgagg atcagatcaa cgtcctcggc
      121 tactgcgtcg gcggtacgct gctggcgacg ctgctggcgt acctggctgc gcggggtgag
      181 gatctttaca agtcggcaac gttccttgcg gcgcaggtcg acttcaacca cgccggcgat
      241 ctgctcgtgt ttatcgacga tgcgcagctg gcggccctgg aagagatgat ggccgaacgt
      301 ggctacctcg acggctcgcg gatggccaca gtgttcaacc tgttgcggcc gaaggacctg
      361 atctggcctt acgtcgtcaa caattacctg ctcggcaaaa agccattccc gttcgatctc
      421 ctgtactgga accaggattc aacgcggatg ccggctgcaa accacaggtt ttacctgcga
      481 gagttctatc atgagaacaa gctggccaag ggcgagatga agattgccgg cacgcggctg
      541 gatctcaagc aggtcaagct tcccatttac gagcttgcga cgcgggagga ccacatcgca
      601 ccggcgaaat cggtgttcgt cggatcgaag ctgttcggcg gcccggtaga gttcgtactt
      661 gcgggctccg gccacattgc cggcgtgatc aaccctccgg acccgaaccg gatcaagtat
      721 cagtattgga ccaacaagaa gccagccgaa acgctggaag aatggatcgc aggagctaag
      781 gagacccccg gttcctggtg gccgcactgg accaaatggc tggccaagca ttccggcaag
      841 cgcgtcccgg cgcgcgagcc cggtgcggtg cttggtgtca tcgaggatgc gccgggtagc
      901 tacgtgaagg tgaaaggctg aggcgctctg gccccactag caggtgcgag gggaagtagt
      961 gccgcgcgtt tgagaattgc ctcaaatgcc gcggatgagg ccgccgtcga tcctcaccac
     1021 ggtgcccgtc acgtagcttg cacgatccga gcaaaggaaa gcagctacgg aaccgaactc
     1081 ctcgggccgg ccatagcgac ctatcggaat gccggcaaga acatcccgcc ggatttcttc
     1141 gacgcttcgc ccttcgcgtt tcgctctggc ttcatcaagc gccgctacgc gctctgtgtc
     1201 gatgcggccg ggcagaacaa cgttgcacgt gatgccgtcg gcagcgacct cggccgcaag
     1261 cgtcttattc caggcgagca gcgatgctct caagctgttc gatagtgcca tcccaggaat
     1321 gggctgcacg gcggctgagg acgcgatcgt aagaagccgt ccccacttgc gctcccgcat
     1381 ggccggaagg gccagagtgg ccgcatggat gaggttgccg accatgcgct cgaaccactc
     1441 gctccaggct ttgtctgaaa cctcgagggc cggcgcgggt ggcggcccgc cgctgttcag
     1501 gacgagaata tcgacgccac ccatttcgcc cactgctgcc tgaaacagat catcaatggc
     1561 agcgcggtcg tgaagatcac agtcacgccc catcacggcg ccgctggtct cgcgggaaat
     1621 ctccgctgcc gcctgcgcgg caccaaaacg gccgcagatc atgacggagg taccttcccc
     1681 tgccagggcc gtggccacgg cgcggccgag acccttcgtc gaagcgagca cgagcgcccg
     1741 gcgccccttc agtccaaggt ccactgcttc ctcctatctg ccgacggcgt agccttgcat
     1801 tccgcggggg ttcgcggcag cacgcaggta aagcgtcccg ccgcgatcct cgcgcgagca
     1861 ggccgaaaga cggccttcgg accagtcctc accaaccacg acatcgtgcc cgcgagcctt
     1921 cagctcagcg acggtttcag gcgggaaccg cccctccagc accagccggt tcggccgtgc
     1981 ctcccgcggc cagaaagagc tcgggcaatg ctcactatga aatgacgggg catcgatcgc
     2041 ctcctgcaaa ttcagtccgt gatggacatg ccgcaggaag agaatgagtg accactgatc
     2101 ctgctggtcg ccgcctggcg tgccccaggc cagataaggc ttgccgtctc gcagggccat
     2161 ggatggcgtg agcgtcgtgc gcgggcg
//
LOCUS       DASUMG010000139         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_579656, whole genome shotgun sequence.
ACCESSION   DASUMG010000139 DASUMG010000000
VERSION     DASUMG010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579656"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VFX46_02250"
     CDS             complement(<1..307)
                     /locus_tag="VFX46_02250"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5599436.1"
                     /translation="MVFTRKPEPRSGNIDPLQIKTMDQATGTPTPTPIRTNSYSGTSG
                     QDANESVIGNDLTIEGQAITIRCQGSLRVNGNIQADLHSVKLTVGEEAVISGTIAAET
                     "
     gene            complement(435..>2575)
                     /locus_tag="VFX46_02255"
     CDS             complement(435..>2575)
                     /locus_tag="VFX46_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX5599437.1"
                     /translation="GRARASQILAVTFTNKAAREMKERVGQLIGGAVEGMPWLGTFHS
                     IGARILRTHAELVGLKSSFTILDTDDQLRLMKQVIEAGGVDSDRWPARQLAALIDSWK
                     NRGLSPSQVPAGESFAFANGKGATLYADYQKRLKELNAVDFGDLLLEGLRLFREHPDV
                     LADYQRRFRFMLVDEYQDTNVAQYLWLRLLAQGSTSPNLCCVGDDDQSIYGWRGAEVE
                     NILRFEKDFPGAKVIRLERNYRSTGHILGAASGLIAHNRGRLGKTLHTDDGLGSKVIV
                     TGVWDDEEEARTIGEHIEAMQRAGQPLNEIAILVRASFQMRAFEDRFMTLGLPYRVIG
                     GPRFYERQEIKDAIAYLEVTLNPANDLKFERIINVPKRGLGDTTMKRIHALARAKDVP
                     LYHAARAITETEEISGKARKSLIDLLNSFERWRGLVGSMKHTELAELILDESGYTAMW
                     QQDRSPQAQSRLENLKELIRFMDEFESLAGFLEHVSLVMDADQTDTDDRVSLMTLHAA
                     KGLEFDVVFLPGWEEGLFPHQRSLDENGQAGLEEERRLAYVGLTRARRQVFISFAQNR
                     RTRGLYQPAIPSRFVDELPPEHVEVVEAPSRFSGPYAEFDRFAANPYGRSRFDDLGGS
                     FQANYQTPGWQRAQQRWRQGERPSRGPLTIEGELVASSTGRSSFQGGERVFHQKFGYG
                     LVAEVDGNKLTVDFDKAGRKRVVDSFVDRV"
BASE COUNT          446 a          835 c          793 g          501 t
ORIGIN      
        1 ccgtctcggc ggcgattgtg ccgctgatga ccgcctcctc accgaccgtc agcttcacgc
       61 tgtgcagatc agcctgaatg ttgccattga cgcgcaatga gccctggcag cgaattgtaa
      121 ttgcctgccc ctcaatggtg aggtcgttgc cgattacgga ctcgttagcg tcctgcccgg
      181 acgtgccaga atagctattg gtgcgtatcg gagttggtgt tggagtgcct gtcgcctgat
      241 ccattgtctt gatctgcagc gggtcgatgt tgccggacct cggctcgggc ttgcgtgtga
      301 agaccattac tctgaactcc cctcgagtac tatttccggc gcgagcatcc gctccggagg
      361 tggtcgaatt gtgtcattcg accacggaag ttcaagtccg aagcttcagc cggcagactt
      421 tacgggagta ccctttagac tcgatcgacg aagctgtcga caacgcgttt gcggcccgcc
      481 ttgtcgaagt cgaccgtcag cttgttgcca tcgacctcgg caaccagacc gtacccgaac
      541 ttttggtgga agacccgctc tcccccttgg aatgacgacc ggccggtcga ggaggcgaca
      601 agctcccctt caatcgtcag tggtccgcgg ctcggccgct cgccctgccg ccagcgctgc
      661 tgcgcccgct gccaacccgg cgtctggtag ttggcttgaa agcttccacc gagatcgtcg
      721 aaacggctcc gaccgtaagg attggcggca aaccgatcga actcagcgta gggaccgctg
      781 aacctgctcg gcgcttccac cacctcgaca tgctcaggcg gcagctcatc gacaaagcgc
      841 gacggaatgg ctggttggta caatccccgt gtgcggcggt tctgggcgaa cgagatgaac
      901 acctgccgcc gggcgcgcgt cagacccaca taggccaagc gcctttcttc ctcgaggccg
      961 gcctggccat tctcgtcgag tgagcgctga tgcgggaaca gcccctcctc ccagccgggg
     1021 aggaagacca cgtcgaactc gagccccttc gccgcgtgca gcgtcatcaa cgacacgcgg
     1081 tcgtcggtgt cggtttgatc cgcgtccatg acgagcgaga cgtgctcgag gaagccggcg
     1141 agcgactcga actcgtccat gaagcggatc agctccttca ggttctcgag ccgcgactgc
     1201 gcctggggcg agcgatcctg ctgccacatg gccgtgtagc cggactcgtc gaggatcagc
     1261 tcggcgagct ccgtatgctt catcgaaccc acgaggccac gccagcgctc gaatgagttg
     1321 agcaaatcaa tgagcgactt gcgcgctttg ccgctgatct cctccgtctc ggtgatggcg
     1381 cgcgccgcgt gatagagggg cacgtccttt gcgcgggcca gcgcatggat gcgtttcatc
     1441 gtggtgtcgc ccagcccgcg cttcggcacg ttgatgatgc gctcgaactt gagatcgttg
     1501 gcgggattga gcgtcacctc caggtaggcg atcgcatcct tgatctcctg gcgctcgtag
     1561 aatcgcggac cgccgatcac ccgatagggc agacccagcg tcatgaaccg gtcctcgaat
     1621 gcgcgcatct ggaacgaagc tcgcaccagg atggcgatct cgttgagcgg ctgtccagcg
     1681 cgctgcatcg cctcaatgtg ctcgccgatc gtgcgcgcct cctcctcgtc gtcccagacg
     1741 ccggtgacga tcaccttgga gccaaggcca tcgtccgtat gcagcgtctt gccgagccgg
     1801 ccccggttgt gcgcgatgag cccggacgcc gcaccgagga tgtggccggt ggagcgataa
     1861 ttgcgctcga gccggatcac cttcgcgccc gggaagtcct tctcgaaccg cagaatgttc
     1921 tccacctcgg cgccgcgcca gccgtagatg gactggtcgt cgtcgccgac gcagcacaga
     1981 ttgggcgatg tcgagccctg ggccagcagc cgcaaccaga gatactgggc gacattggtg
     2041 tcctggtact cgtccaccag catgaagcga aagcggcgct gatagtcggc caggacgtca
     2101 gggtgctcgc gaaacagcct gaggccttcg agcaataagt cgccgaaatc aactgcgttg
     2161 agctccttca ggcgcttctg gtagtcggca tagagcgtcg cccccttgcc gttggcaaag
     2221 gcgaagctct cacctgccgg cacctggctt ggcgacagcc cccgattctt ccagctgtcg
     2281 atcagcgcag ccagctgacg ggctggccag cgatcgctat ccacgccacc cgcctcgatg
     2341 acctgcttca tgagacggag ctggtcatcc gtgtcgagga tcgtgaagct gctcttcaag
     2401 cccaccagct ccgcgtgggt gcgcagaatc ctggcgccga tcgagtggaa cgtgccgagc
     2461 cagggcatgc cttcaacggc gccgccgatg agctgcccga cgcgctcctt catctcgcgc
     2521 gccgccttgt tggtgaaggt gacggcgagg atctggctcg ctcgtgcgcg gcctg
//
LOCUS       DASUMG010000140         3319 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1142341, whole genome shotgun sequence.
ACCESSION   DASUMG010000140 DASUMG010000000
VERSION     DASUMG010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142341"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VFX46_02260"
     CDS             <1..527
                     /locus_tag="VFX46_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599438.1"
                     /translation="INTRGSFLVSKCCIPHLKRAENPHILMLSPPLDMQPKWFAGHVA
                     YSIAKFGMSLCVLGLAEELKPDGIAVNALWPRTTIATAAIRNLLGGEEVVRMSRKPDI
                     LADAAHLVFTQPARSFTGHFLIDDTFLYEVGGVADFDQYRVDPTIDLAPDFFVPESST
                     PPPGVRLAARDVRA"
     gene            603..1550
                     /locus_tag="VFX46_02265"
     CDS             603..1550
                     /locus_tag="VFX46_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEX5599439.1"
                     /translation="MPALLITHPSFVEHDTGPGHPERPDRMRAIDKALSHEMFNDLKR
                     EEAPLREDVEERILLAHPKRYLEAIKAARPAEGEDPVRLDPDTVLSSTSWEPALRAVG
                     AGLLAVDQVLDPSSGIRKAFCQIRPCGHHAEKERAMGFCIFNNIAIAGLYAREKHGVE
                     RVAVVDFDVHHGNGTQDIFWSDKNLFFASTHQMPLYPGTGAESERGVGNIFNAPLRPN
                     DGGEQFREAMRSRILPALQNFSPDIILISAGFDAHEADPLASLRLHEDDYAWITDELC
                     EAAEKSAQGRVVSMLEGGYDLTALAQSVAVHVKLLMDAA"
     gene            1585..1848
                     /locus_tag="VFX46_02270"
     CDS             1585..1848
                     /locus_tag="VFX46_02270"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX5599440.1"
                     /translation="MANGTEKYPDLKDMSFERALKELEAIVGRLERGDVELEESIAIY
                     ERGEALRDHCDRLLKQAEAKVEKLTFGADGQPQGTEPLDSSSK"
     gene            1979..>3319
                     /gene="dxs"
                     /locus_tag="VFX46_02275"
     CDS             1979..>3319
                     /gene="dxs"
                     /locus_tag="VFX46_02275"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX5599441.1"
                     /translation="MKPGSTTPLLDTVRYPEDLRRLPEEDLPQLAAELRQETIDAVSV
                     TGGHLGAGLGVIELTVALHWVFDTPRDRLIWDVGHQAYPHKILTGRRDRIRTLRQGGG
                     LSGFTKRSESEYDPFGAGHSSTSISAGLGMAVARDLAGKKHNVICVIGDGAMSAGMAY
                     EAINNAGAMDSRLIVILNDNDMSIAPPTGALSAYLARITSSGTYFYVRDIAKQLAKRL
                     PKSWERRAARVEEYTRHLWQGGTWFEELGFYYVGPIDGHNFDHLLPVLKNVRDADQGP
                     VLVHVVTRKGKGYPPAENSADKYHGVNRFNVITGDQFKPKPNAPSYTRVFAESLIEEA
                     KKDDKIVAITAAMPSGTGLDLFGRVFPERTFDVGIAEQHAVTFAGGLATEGFKPFAAI
                     YSTFLQRAYDQVVHDIAIQKLPVRFALDRAGLVGADGPTHAGSFDLAYLGCLPGF"
BASE COUNT          613 a         1029 c         1064 g          613 t
ORIGIN      
        1 gcatcaacac gcgcggttcc ttcctggtct cgaaatgctg catcccgcac ctcaagcgcg
       61 ccgagaaccc gcacattttg atgctctcgc cacctcttga tatgcaaccc aagtggttcg
      121 ccggccacgt cgcctattcc atcgccaagt ttggaatgag cctatgcgtg cttgggctgg
      181 cggaggagct gaaaccggat ggaattgccg tcaacgcgct gtggcctcgg acgaccatcg
      241 cgacggcagc gatccggaat cttctcggcg gcgaggaggt ggtacggatg agccgtaaac
      301 ccgatattct ggctgatgcc gcgcatctgg tcttcactca gccggcccgt tcgttcaccg
      361 gtcactttct gatcgatgac acgttcctgt acgaggttgg cggcgtggcc gatttcgacc
      421 aataccgggt cgacccgact attgatctgg cgccggattt ctttgtgcca gaaagcagca
      481 cgccgccacc cggcgttcgg ctcgccgcgc gcgacgttcg cgcctgatcg tcagtgcggg
      541 cactcgtcgc caacttggac tagaatggcc atttcgaccg cttggccgag ggacttagga
      601 tcatgccagc cttgctcatc acgcatcctt catttgttga acacgacacc ggccctgggc
      661 atccggagcg gccggaccgc atgagagcta tcgacaaggc gctctcgcat gagatgttca
      721 acgacctcaa gcgcgaggag gcgccgctgc gcgaggacgt cgaggagcgg attctgctgg
      781 ctcatccgaa gcgttacctc gaagcgatca aggccgcgcg gccggcagag ggtgaggatc
      841 ccgttcggct cgatcccgac accgtcctgt cttcaacgag ctgggagccg gcgctgcggg
      901 ccgtcggcgc ggggctgctc gcggtagatc aggttctcga tccatcatcc ggcatccgca
      961 aagctttctg ccagattcgc ccctgcggcc atcacgcgga gaaagaacgg gcgatgggct
     1021 tctgcatctt caacaacatc gcgatcgctg gcctttacgc ccgggagaag cacggcgtcg
     1081 agcgcgtagc cgtcgtggac ttcgacgtgc atcacggcaa cggaacgcag gatatcttct
     1141 ggtccgacaa gaacctgttc ttcgcctcga cgcaccagat gccgctttat ccagggacgg
     1201 gcgccgaatc ggagcgaggg gtcggcaata ttttcaatgc gccgctgcgg ccaaacgacg
     1261 gtggcgagca gtttcgggag gcgatgcgct cgcggatcct gcccgctttg cagaacttct
     1321 caccggacat cattctgatt tccgccggct ttgacgcgca cgaagcggac ccgctcgcca
     1381 gccttcgtct gcatgaggac gactatgcct ggatcacgga cgagctgtgc gaggcggccg
     1441 agaaatcagc ccaagggcgg gtggtctcga tgctggaagg gggttacgat ctgacggcgc
     1501 tggcgcaatc agtggccgtc cacgtcaagc tgctcatgga cgcggcgtga cggcggagga
     1561 ggaccataca cggcggaaga gggtatggcg aacggaacag agaaatatcc tgatttgaag
     1621 gacatgagct tcgagcgagc gctcaaggag ctcgaggcga tcgtcggccg gcttgagcgc
     1681 ggggacgtgg agctcgagga aagcatcgcc atctacgagc gaggcgaggc gctgcgcgac
     1741 cattgcgacc ggctgttgaa gcaggcggag gccaaggtcg agaagctgac gttcggagct
     1801 gacggacagc cgcagggcac cgagcctttg gactcttcct cgaaatgaca ggacgcgcgc
     1861 ggatcaagcg tgtttcccgc gtcgatgctg ccagatgccg cgcgtcctga gacgagctat
     1921 actgagcgac cgcttgctcg ttcctggtgc cccctaggtc ggcagaaagg atcaccccgt
     1981 gaaacctggt agcacaacgc cgttgctcga tacggttcgc tatccggagg acctgcgccg
     2041 gttgccggaa gaggatcttc ctcagcttgc ggccgagttg cgccaggaga cgatcgatgc
     2101 cgtgtcggtg acgggcggtc acctcggcgc gggtctcggg gtcatcgagc tcacggtggc
     2161 cttgcactgg gtcttcgaca cgccacggga tcggctgatc tgggatgtcg gtcatcaggc
     2221 ctatccccac aagatcctta cggggcgccg cgatcgcatc cgcacgctcc gccagggtgg
     2281 cggcctctcg ggcttcacga agcgttccga aagcgaatat gacccgttcg gcgcggggca
     2341 ctcgtcgacc tcgatctctg ccgggctcgg aatggcggtc gcgcgcgacc ttgcgggaaa
     2401 gaagcacaat gtcatctgcg tgatcggcga cggtgcgatg agcgctggca tggcctacga
     2461 ggcgataaac aacgccggcg cgatggacag ccgtttgatt gtcatcctta acgacaacga
     2521 tatgtcgatc gcgccgccga ccggggcact gtccgcctat ctggcgcgga tcacctcgag
     2581 cggaacttat ttttacgtcc gcgacatcgc caagcagctt gccaagcgcc tgcccaagag
     2641 ctgggagcgc cgtgcggcac gggtggagga gtacacccgc cacctctggc agggtggcac
     2701 gtggttcgag gagctggggt tttactacgt cggccccatt gatgggcaca acttcgacca
     2761 cctgctgccc gttctgaaga acgtgcgcga tgcagaccag gggccggtgc tcgttcacgt
     2821 ggtcacgcgc aagggcaaag gctatccgcc ggctgaaaat tcggccgaca agtaccacgg
     2881 cgtgaaccgc ttcaacgtca ttacgggtga ccaattcaag cccaagccca acgcgccaag
     2941 ctacacccgc gtgttcgccg agagcctgat cgaggaggcc aagaaggacg acaagatcgt
     3001 tgccatcacg gcggcaatgc cttcgggcac ggggctcgac ctattcggca gggtgtttcc
     3061 ggagcggacc ttcgatgtcg gcattgccga gcagcacgcg gtgaccttcg ccgggggtct
     3121 ggcgacggag ggcttcaagc cgtttgccgc gatctactcg accttcctgc agcgcgccta
     3181 tgaccaggtg gtgcacgaca tcgccatcca gaagctgccg gtgcgcttcg cgctcgaccg
     3241 cgccggcctg gtcggcgccg atgggcccac ccatgcgggc tccttcgatc tcgcctatct
     3301 cggctgcctt cccggcttc
//
LOCUS       DASUMG010000141         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_18253, whole genome shotgun sequence.
ACCESSION   DASUMG010000141 DASUMG010000000
VERSION     DASUMG010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18253"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="fdxH"
                     /locus_tag="VFX46_02280"
     CDS             <1..835
                     /gene="fdxH"
                     /locus_tag="VFX46_02280"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEX5599442.1"
                     /translation="RQLTPVAKLIDVSKCIGCKACQAACLEWNDLREEVGVNHGVYDN
                     PLDLTPASWTVMRFTEYENGDDLEWLIRKDGCMHCDDPGCLKACPAPGAIVQYTNGIV
                     DFVSENCIGCGYCVKGCPFNIPRISKVDNKAYKCTLCSDRVAVGLGPACQKACPTQAI
                     VFGPKDEMKKWAEKRIEDLKSRGYDKAGLYDPPGVGGTHVMYVLHHADKPELYSGLPN
                     DPKISPVVEAWKGLSKYAGMAVLGLTAAFGFIHYILQGPNRVTDEDEENAEKLAGGGT
                     Q"
     gene            832..1512
                     /locus_tag="VFX46_02285"
     CDS             832..1512
                     /locus_tag="VFX46_02285"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HEX5599443.1"
                     /translation="MSQTKTSARVRVRRYSGSARINHWIVAISFVLLMLSGLSLFHPT
                     FFPLSALFGGLQVAREVHPWLGLLLAVGFLGLFIRFFPQNLPERTDWVWLARLRHVLT
                     ANDHYLPEVGKYNAGQKFVFWSQFVLIATLLVTGIGLWDQGLAYVESALGFKATIEQQ
                     RWAALIHSIAAVLAIAIWIVHVYAAIWVRGTISAMTRGTVTGGWGWRHHRKWLRKEVE
                     KGGVEKTA"
     gene            1592..2509
                     /gene="fdhE"
                     /locus_tag="VFX46_02290"
     CDS             1592..2509
                     /gene="fdhE"
                     /locus_tag="VFX46_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX5599444.1"
                     /translation="MSESGSAKLYTPNIGEAAKPPPVVLPYPASLFLNRSKRLLGLAP
                     GHQLESYLRFLAAVTRAQHDIQAALPEATPPSAEAVTQAVAHGMPPIPRTSSKPDEQA
                     ETTIERFLDSLTKLELPAEAAAAAKSLQSMTSEQRRVFINNVLDETDIQDRIAQSALI
                     LAGLQVHFTRLAAKLDASVLKPFADGVCPTCGSRPIASSVVNWPGATNTRYCTCSLCA
                     TMWYVVRVKCLLCGSNESINFRLIEGQPDTIKAEACDKCHGYVKIFYQIKDPTLEPLA
                     DDVASLGLDMLLTQDGWSRGGYNPFLIGY"
     gene            2548..>3167
                     /locus_tag="VFX46_02295"
     CDS             2548..>3167
                     /locus_tag="VFX46_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX5599445.1"
                     /translation="MNDTLSMPPARLPSVEQVLRTDVGTVATERFGHEATVKAVRRVL
                     DDIRRTLGFAGTNLPDKQTVAAEALELLEREHAPSVRRVFNLTGTVLHTNLGRAILPE
                     VAIEAAVLAMREPVALEFDVDSGTRGERDDHVRGLISELTGAEDAAIVNNNAGAVLLV
                     LNTFARGKEAIVSRGELIEIGGSFRLPDIMARAGADLVEVGTTNRT"
BASE COUNT          611 a          971 c          976 g          609 t
ORIGIN      
        1 ccgccagctg acgcctgtcg ccaagctcat cgatgtgtcc aagtgcatcg gctgcaaggc
       61 gtgccaggcg gcatgccttg agtggaacga tctgcgcgag gaggtcggcg tcaaccacgg
      121 ggtctacgac aacccgctgg acctgacgcc ggcctcgtgg accgtgatgc gcttcacgga
      181 atacgagaac ggcgacgatc tcgagtggct gatccgcaag gacggctgca tgcactgcga
      241 cgacccgggc tgcctgaagg cctgcccggc gccgggcgcg atcgtgcagt acaccaacgg
      301 catcgtcgac ttcgtgtcgg agaactgcat cggctgcggt tactgcgtga agggctgccc
      361 cttcaacatc ccgcgcatct ctaaggtcga caacaaggcc tacaaatgca cgctctgctc
      421 ggaccgcgtc gcggttggac ttgggcctgc atgtcagaag gcttgcccga cccaggccat
      481 tgttttcggc ccgaaggacg agatgaagaa gtgggctgag aagcggatcg aagacctcaa
      541 gtcgcggggc tacgacaagg ctggtctcta tgatcctccc ggcgttggcg gcacgcacgt
      601 gatgtacgtc ctgcatcacg ccgacaagcc tgagctttat tcgggactgc ccaacgatcc
      661 gaagatcagc cccgttgtgg aagcctggaa ggggctcagc aaatacgctg gaatggccgt
      721 tctcggcctg acggccgcct tcggcttcat ccactacatt ctgcaagggc cgaaccgcgt
      781 gaccgatgag gacgaagaaa acgcggagaa gcttgcggga ggtggcacac aatgagtcag
      841 acgaaaacct cggcgcgcgt ccgcgtgcgc cgatattcgg gaagcgcccg catcaatcat
      901 tggatcgtgg cgatcagctt cgtcctgctg atgctgagcg ggctctcact gtttcacccg
      961 accttcttcc cgctgagcgc gctgttcggc gggttgcagg tcgcacgtga agtgcatccg
     1021 tggctcggcc tactgcttgc ggtcggattt ctcggcttgt tcattcgctt ctttcctcag
     1081 aacctgccgg aaaggaccga ctgggtgtgg cttgcccgat tgcggcatgt cctgacggcc
     1141 aacgaccatt acctgccgga agtcggcaag tataacgctg ggcagaagtt cgtcttctgg
     1201 tcccagttcg tgcttatcgc cacccttctc gtcaccggca tcgggctttg ggatcagggc
     1261 cttgcctatg tcgagagcgc gctagggttc aaggcgacaa tcgaacagca acgctgggca
     1321 gcgttgatcc actccatcgc agctgttctc gccatcgcga tctggatcgt ccacgtttac
     1381 gcggcgatct gggtgcgtgg caccatcagc gccatgacgc gcggcacggt gaccggcgga
     1441 tggggctggc ggcaccaccg aaagtggctg cggaaggaag ttgagaaagg cggtgtggag
     1501 aaaactgctt agtgttatgc tggcagggta accggcggcc cctttagaag gaggggccgc
     1561 taatcataca catcctcagt ttgaggtgga catgtctgag tcaggctctg ccaagcttta
     1621 tactccgaac attggggagg cggctaagcc gccgccagtc gttcttccct atccagcttc
     1681 tctcttcctg aaccggtcga agcgcctcct aggtctggca ccggggcatc agctcgagag
     1741 ctatttgcgc ttcttggcgg ccgtcacgcg agcgcagcat gacatccagg ccgcattgcc
     1801 cgaagcgact cctccctcag ctgaagcggt tacccaggct gtcgcgcatg gtatgccgcc
     1861 catcccgcgc acctcctcca aaccagacga gcaagccgag acgacaatcg agcgttttct
     1921 cgacagcctg accaagctcg agctgccggc agaggccgcc gctgccgcca aatcattgca
     1981 aagcatgacg agcgagcagc gccgggtctt tatcaataat gtgctcgacg aaaccgacat
     2041 tcaggacagg atcgctcaga gcgcgcttat cctcgcaggg cttcaagtcc atttcacgcg
     2101 attggcggca aagcttgatg ctagcgtatt gaagcctttc gcagatggtg tctgcccgac
     2161 gtgcggtagc cggcccatcg cgagctccgt cgtcaactgg ccgggtgcca ccaacacgcg
     2221 ctattgcaca tgctcccttt gcgcgacgat gtggtacgtc gtgcgcgtaa agtgtctgct
     2281 gtgcggctcg aatgagagca tcaacttccg ccttatcgag gggcagccgg atacgatcaa
     2341 ggctgaggcc tgcgataagt gccacgggta cgtgaagatc ttttaccaga tcaaagaccc
     2401 gacgctggag ccgctggcgg acgacgtcgc ctctctgggt ctcgacatgc tgctgaccca
     2461 agatggctgg tccaggggtg gctataatcc attcctgatt ggctactgac gcgctcctta
     2521 tatcggggcg cccagcgagc accgacgatg aacgacacgc tctccatgcc gccagcgcgc
     2581 ctgccatctg tcgagcaggt gttgcgaacc gacgtcggca cagtcgccac cgagcgtttc
     2641 ggccacgagg cgaccgtcaa ggccgtgcgg cgcgtgcttg atgacattcg gcggacactc
     2701 ggatttgccg gaacgaactt gcccgataag cagacggtgg ctgccgaggc tctggagctg
     2761 ctcgagcgtg agcacgcgcc cagcgttcgc cgcgttttca atctcacagg caccgtgctg
     2821 cacacgaacc tcggtcgcgc aattctgccc gaggtggcga tcgaagcagc cgtcctagcc
     2881 atgcgtgagc cggtggcact cgaattcgat gtcgacagcg gcacccgtgg cgagcgggac
     2941 gaccacgtgc gcgggctgat ctcagaactg acgggcgcgg aagacgctgc catcgtcaac
     3001 aacaatgccg gcgctgttct gctggtgctc aacacgttcg cgcgaggaaa agaggcaatc
     3061 gtttctcgcg gcgagctgat cgagatcggc ggctcattcc ggcttccgga cattatggcc
     3121 cgcgccggcg ccgacctcgt cgaagtcgga acgacgaacc ggacgca
//
LOCUS       DASUMG010000142         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1062291, whole genome shotgun sequence.
ACCESSION   DASUMG010000142 DASUMG010000000
VERSION     DASUMG010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062291"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..499)
                     /locus_tag="VFX46_02300"
     CDS             complement(53..499)
                     /locus_tag="VFX46_02300"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5599446.1"
                     /translation="MSKSQRKPPARRAQFKHFTVIPTRWMDNDVYGHVNNVVYYSYFD
                     TVVNRYLIDFCGLDYHSGETIGVVVETSCRFHRSFCFPEDIEAGLRVAHIGNSSVNYE
                     IGLFGVGEDEARADGYFVHVYVDRATRRPTPMPDRMRIALARLAEG"
     gene            complement(559..906)
                     /locus_tag="VFX46_02305"
     CDS             complement(559..906)
                     /locus_tag="VFX46_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5599447.1"
                     /translation="MTITRRETTEILSNVVEANGFVFTAGITAKDTSRDVKGQTEEIL
                     KEIERLLGLCGTDKSKIVSTTIWVSDIRHREPMNEVWRAWTGGKDLPARACVEAKLAD
                     PRLLVEIAVIAAK"
     gene            complement(1058..1855)
                     /locus_tag="VFX46_02310"
     CDS             complement(1058..1855)
                     /locus_tag="VFX46_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5599448.1"
                     /translation="MANGSDAVLGVTRDATTASDAQSENISARAPWHERHPLNGQANG
                     ARSNGAGIASRELQAQDDAAPVFRDRARTILPVEYQGREVVFEAYAYEDDSASYQALA
                     LVHRATSSSADELPLVRVHSGCATGDIFHSLRCDCHAQLQKAIGRVIESRIGAIIYLP
                     HHEGRGIGLVAKIKAYAEQDRGLDTVDANISIGAPVDARDYTLAAKILKDLGFTEIRL
                     LTNNPLKVEALQRLGIKVAEQIQHATSPSRYNARYLATKRERMAHKL"
     gene            complement(2335..3309)
                     /gene="denD"
                     /locus_tag="VFX46_02315"
     CDS             complement(2335..3309)
                     /gene="denD"
                     /locus_tag="VFX46_02315"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX5599449.1"
                     /translation="MKVLITGGAGMIGRKLAQRLARDGSLAGRPITSVVLHDVVEAPR
                     CPDCPFPMTVKVGDLSRPGEAEELVADRPDVIFHLAAVVSGEAELDFDKGYRINLDGT
                     RYLFEAIRKAGNKPRVVFTSSIAVFGAPFPEAIGDEFFTTPLTSYGTQKAICELLLAD
                     YTRKGFLDGIGIRLPTICVRPGKPNKAASGFFSSIIREPLAGQEAVLPVSEDVRHWHA
                     SPRAAVGFLLHAAALDGDAVGPRRNLTMPGVSVTVGEQIEALRKVAGDKVVARIRREP
                     DPLIMQIVSGWPRNFDARRAIELGFRADSSFEEIIRIHIEDELGGKIA"
     gene            complement(3360..4136)
                     /gene="otnI"
                     /locus_tag="VFX46_02320"
     CDS             complement(3360..4136)
                     /gene="otnI"
                     /locus_tag="VFX46_02320"
                     /EC_number="5.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HEX5599450.1"
                     /translation="MPRFAANLSMMFTEVPFLDRFEAAAKAGFKAVEFLFPYEHPAEE
                     IAARLEANGLTLVLFNLPPGDWTKGERGLAALTGREADFEAALREALKYAEALRCPRV
                     HAMAGLVHHGTSRATYVANLRKAARMAKDFGVEVIIEPINTRDIPGYFLNHTSEARAI
                     IYEVGEPNLGLQFDIYHRQIVEGDVATAIREYAHLTRHYQIANPPDRGEPDEGELNYA
                     WLFKLIDETGFDGWIGCEYRPRRGTIEGLKWVERCGVRLG"
BASE COUNT          821 a         1299 c         1237 g          842 t
ORIGIN      
        1 cagcattcag gggccgcacc ggcctacgtc gactggcaag actgccgatg cctcaaccct
       61 cggcgagacg cgccaatgcg atccgcatcc gatcgggcat gggtgtcggc cggcgtgtcg
      121 cccgatcgac gtacacatgc acgaaatagc catcagcgcg agcctcgtcc tcaccaacgc
      181 cgaacagtcc tatctcgtaa ttgaccgacg agttgccaat atgggcgacg cgtaaaccgg
      241 cttcgatgtc ctcgggaaag cagaaggagc ggtgaaagcg acaactggtc tcaacgacga
      301 cgccgatggt ttcacctgag tgataatcga ggccgcaaaa atcgatcaga taccgattaa
      361 caactgtatc gaaatacgag tagtacacca cgttattgac gtggccgtag acgtcgttgt
      421 ccatccagcg cgtaggaatt acggtgaagt gcttgaactg cgcgcgccgg gcaggaggct
      481 tgcgttggct cttgctcatg agtctgtctg gggggtgggc cggaagatgt tcagctaatc
      541 cggccctgtt atgaataact acttcgcggc aataacagcg atttcgacca aaagccgcgg
      601 gtccgcgagc ttcgcttcca cacaagcacg tgccggcagg tccttgccgc ccgtccaggc
      661 ccgccagact tcattcatcg gctcgcgatg ccgaatgtcg gacacccaaa tggttgtaga
      721 gacgatcttg ctcttgtccg tgccgcacaa gcccaagagt ctctcgatct ctttgaggat
      781 ctcctcggtc tgccccttca cgtcccggct tgtatctttg gccgtaatgc cggctgtgaa
      841 cacgaagccg ttcgcctcca ccacgttgga taggatctcc gttgtctcgc gacgtgtgat
      901 ggtcatgggc ttctccccgt gccttacgtt gaacagaagc aactccttag cacgcttggc
      961 aacttctgcc agcgggtcag gcttttgtaa gccgcgcgaa caggatcttc cgacggaaac
     1021 ggtcgcgctc gatctggcgc ggacgagcag ccgctcatca cagcttgtga gccattctct
     1081 cccgcttggt cgcgagataa cgcgcgttgt agcgtgatgg cgatgttgcg tgctggatct
     1141 gctcggcgac cttgattccg agccgctgaa gcgcctcgac tttcaaagga ttgttcgtga
     1201 gcagcctgat ttctgtaaag cccaggtctt tgaggatctt cgccgccaaa gtgtagtctc
     1261 gggcatcgac gggcgcgccg attgaaatat tcgcatccac ggtgtcgagg ccgcggtcct
     1321 gctccgcata tgcctttatc ttcgcgacaa ggccgatgcc gcgcccttca tgatgcggca
     1381 gatagataat cgcgccaatc cggctttcga tgacccgccc tatcgccttt tggagctggg
     1441 catggcagtc gcagcggaga gaatggaaga tgtcgcccgt ggcgcagcca ctgtgcacgc
     1501 gcacaagggg cagctcgtcc gcacttgacg atgttgcgcg gtgaacgagc gccagcgcct
     1561 gataggaggc cgagtcatcc tcgtaggcat aggcctcgaa aacgacctcg cggccctgat
     1621 actcgaccgg caggatggtc cgcgcccgat cccggaacac cggagcggcg tcgtcttgcg
     1681 cctgcagctc acgagaagcg atcccggcac cgttcgaccg cgcgccattg gcctggccat
     1741 tcaatggatg acgctcgtgc cagggtgctc gcgccgaaat gttttccgac tgcgcgtcgg
     1801 aagctgtcgt cgcgtcccgc gtcacaccca gcactgcgtc tgaaccgttc gccatagtgg
     1861 tcctcgatga atcatctcgt cgtaacaaat ctgcccaagg cagttgttaa ggcgacgtaa
     1921 tcgtcggtgc tgctttttcg atcggctggg ccaaccgcac acctcctgag aggtacgaac
     1981 gcggccctac cgaaaacggc ccctcgctcg ccccaacgga acgcgactaa catttcggtc
     2041 ccgacctcaa acgtcgccat cgcgcggttg ctcctcgcgt gatcgacaga acgttagcgc
     2101 cgaacactgt ggcgccatta gggtgaaagg cctctcgtcg gtacactgac tgcctcatgc
     2161 cggacggtat gggataagct ccttcttctg tcagccggag gcgtaaaggt ggtgggcgcc
     2221 tcgcacgtga tggtagccaa ttgttctatt gctttcgcat cgttcaacaa atgggggccg
     2281 aagaacgccc ttaccagggg aggttcgcgt tggtgaaccc tcagcccttc acaatcatgc
     2341 tatcttgccg ccgagctcgt cctcgatgtg tatgcggatg atctcttcaa agctcgagtc
     2401 cgccctgaag ccgagctcga tagcccgccg cgcgtcgaag ttacgcggcc agccggaaac
     2461 aatctgcatg atcagcgggt ccggctcgcg acgaatgcgc gctacgacct tatcgccagc
     2521 gactttgcgc agagcctcga tctgctcgcc gacggttaca gagacgcccg gcatggtcag
     2581 gttgcgcctg gggccgacag catcgccgtc gagcgccgcg gcgtgcagca gaaagccgac
     2641 agcggcccgc gggctggcgt gccagtggcg tacatcctcg gaaacgggaa gcacggcctc
     2701 ctgcccggcc agcggctcgc gaatgatgct ggagaaaaag cccgaagcgg ccttgttggg
     2761 cttgcccggc ctcacgcaaa tcgtcggaag ccggatgccg atgccgtcga gaaatccctt
     2821 gcgggtgtaa tcggccagca gcagctcaca gatggctttt tgagtcccgt aggaggtcag
     2881 cggcgtcgtg aagaattcat cgccgatcgc ctcgggaaac ggcgcgccga acacagcgat
     2941 ggagctggtg aacaccacac gcggcttatt gccggccttg cgaatggctt cgaacagata
     3001 cctcgtgcca tcgaggttga tgcgatagcc cttgtcgaaa tccagctccg cctcgcccga
     3061 gacaacggcg gcgagatgga agatcacatc cggcctgtcg gctaccagct cttcggcctc
     3121 tcctgggcgc gagaggtcgc ccaccttgac agtcatcgga aaggggcagt cggggcagcg
     3181 cggggcttcc acgacatcat gcagcacaac gctggtgatc ggtcttcccg cgaggctgcc
     3241 atcacgcgcg agccgctgcg ctagctttcg gccgatcatg ccagcaccgc cggtgatcaa
     3301 gactttcatg gacgttgctc ctccctcggg tcgttagggg tcgagcttcc agggcgggtt
     3361 cagccgaggc gaacgccgca tctctcaacc catttcaatc cttcgatcgt gccgcgccgc
     3421 ggccggtatt cgcaaccaat ccagccatca aagccggttt catcgatcag cttgaacagc
     3481 cacgcgtagt tgagctcacc ctcgtccggc tcgcctcgat ccggaggatt ggcgatctga
     3541 taatggcgcg tcaggtgagc gtactcacgg atcgcggtcg caacgtcgcc ttcgacgatt
     3601 tgccggtgat agatgtcgaa ctggagcccc aggttcggct ctccaacctc gtaaatgatc
     3661 gcccgcgcct cgctcgtgtg gttcaggaag tagcctggaa tatcgcgcgt attgatcggc
     3721 tcgatgatca cttcgacccc aaaatccttc gccatacgcg cagcttttct caggtttgca
     3781 acgtaggtcg cacgcgaggt gccgtgatga acgaggccgg ccatggcgtg aacgcgggga
     3841 caccggagcg cttccgcata tttgagggct tcgcgtaacg cagcctcgaa gtcggcctcc
     3901 cgtccggtca acgccgcgag cccgcgctcg cctttggtcc aatctccagg cggcaaattg
     3961 aaaagcacca gcgtaaggcc gttggcttcc aggcgcgccg cgatttcctc tgccgggtgc
     4021 tcgtaaggga agaggaattc gaccgccttg aaacccgcct tcgctgctgc ttcgaagcgg
     4081 tccaggaaag gcacctcagt gaacatcatg ctgagatttg cagcaaagcg cggcatctgt
     4141 ctcaagcctc ggcaatcagg ttggagttgc agggaccatc gatccgaaag aagacagtt
//
LOCUS       DASUMG010000143         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_741310, whole genome shotgun sequence.
ACCESSION   DASUMG010000143 DASUMG010000000
VERSION     DASUMG010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741310"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1036)
                     /locus_tag="VFX46_02325"
     CDS             complement(<1..1036)
                     /locus_tag="VFX46_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEX5599451.1"
                     /translation="MPPAHPTAATSADVSHKDMANAIRALAMDAVEKAKSGHPGMPMG
                     MADVATVLFTRFLRYDASDPAWPNRDRFVLSAGHGSMLLYALLYLTGYPGMDIEQIKQ
                     FRQLGSKTAGHPEYGHAPGIETTTGPLGQGIATAVGMAIAERIWNARLGPDVIDHHTY
                     VIAGDGCLMEGISQEAITLAGHLGLGKLIVLFDDNGISIDGPTSLSTSEDQIRRFEAA
                     GWNTAAVDGHDPDAVAAAISRARSDGSKPWLIACKTTIGFGAPKKQGTAGVHGSPLGG
                     EEVAAARQALGWHHPPFEVPEPILNAWRIAGRRGAAERASWQERWAKVDAKLRQSFEH
                     PAAAHGEALRQ"
     gene            complement(1242..2141)
                     /locus_tag="VFX46_02330"
     CDS             complement(1242..2141)
                     /locus_tag="VFX46_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX5599452.1"
                     /translation="MKRIICIGHAALDYIYRIEAFPKVPSKVRALEHVESGGGMAANA
                     AATIARLGGKAELWSRVGDDDAGVKIRAGLRSLGVDVRSVQAFEEGRTSTSAIIVDDA
                     GERLIVGARDINMPSGTSWLPLERIKGAGAVLGDVRWLEGLRVSFARARKEGVPTVLD
                     ADLGAREALAEVLELTDYAIFSAPALEDFIPEVEIGEKLKRVLAFGPKHAGVTLGADG
                     YRWMEASGEGHQPGFKVDVVDTTGAGDAFHGAFTLALVEGQPIEACARFACAVAAMKC
                     TRIGSRAGLPTRAQVESFLAARN"
     gene            complement(2265..>3085)
                     /gene="kdsA"
                     /locus_tag="VFX46_02335"
     CDS             complement(2265..>3085)
                     /gene="kdsA"
                     /locus_tag="VFX46_02335"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX5599453.1"
                     /translation="STVTVGSVTFANNRPIALFAGPCQMESRAHALEMAAALKEICGR
                     LGIGLVYKTSFDKANRTSLSAKRGIGLAAALPVFAEIRESLGLPCVTDVHEPAQCAPV
                     AEVVDVLQIPAFLCRQTDLLVAAAKTGRVVKIKKGQFLAPWDMKNVVAKVTGSGNTKV
                     LVTERGVSFGYNTLVVDMRSLPIMAETGAPVIFDATHSVQQPGGQGTSSGGDRRFVPV
                     LARAAVAVGIAGLFIETHQDPDKAPSDGPNMVPLRQLESLLSEVMEIDRAVKKQ"
BASE COUNT          563 a         1004 c          941 g          577 t
ORIGIN      
        1 cctgccgaag cgcctcacca tgcgcagccg ccgggtgctc gaagctctgg cgcagcttgg
       61 catcgacctt ggcccagcgt tcctgccagc tcgcccgctc tgccgccccg cgccgcccgg
      121 caatccgcca agcattcaga ataggctctg gcacttcgaa aggcggatga tgccacccga
      181 gcgcctgtct cgccgctgcg acctcttcgc caccgagcgg ggagccgtgc actccggccg
      241 tcccttgctt ttttggggcg ccaaacccga tcgtggtctt gcaggcgatg agccagggct
      301 tgctgccatc agaacgggcg cgcgagatgg cggcggccac ggcgtccgga tcgtgcccat
      361 cgactgcagc cgtgttccaa ccagcggcct cgaagcgccg aatctgatcc tcggaggtgg
      421 aaagcgaagt gggcccgtcg atggagattc cattgtcgtc gaacagcaca atcagcttgc
      481 ccagcccaag gtgccccgca agcgtgatgg cctcctggct gatgccttcc atcaggcagc
      541 catcgcccgc gatcacatac gtgtggtgat caataacgtc gggcccgaga cgcgcgttcc
      601 agatgcgttc tgcaatcgcc attccgaccg cggtggcaat gccctggcct aacggcccag
      661 tggtcgtctc gatgcctggg gcgtgcccat actcggggtg gcccgcggtc ttcgagccga
      721 gctggcggaa ctgcttgatc tgctcgatgt ccatgcccgg atagccggtc aggtagagca
      781 gcgcgtaaag cagcatggag ccatggccgg ccgagagcac aaagcggtcg cggttgggcc
      841 aggcgggatc ggaagcgtcg taacgcagaa atctggtgaa aagcacagtt gcgacgtcgg
      901 ccatgcccat tggcatgccg ggatgccccg acttggcctt ctcgactgcg tccatggcga
      961 gcgcgcgaat ggcgttggcc atatccttgt ggctcacgtc tgcgctcgtc gcagccgtgg
     1021 ggtgagctgg gggcattggc ttcctcgttg ttgcccggcc ggacggtgca cgccggcccg
     1081 gatcggtatt gcgcgaccca tttagctgca ggcgcgggtg gcgtctacac tatggacagt
     1141 cgcactgacg tggcaggtga agttgtgcaa agcgaccaac tcgatcgcct cgataagccg
     1201 ccattagggg ccctgccggc tggctccgaa aagttgcatc ctcagttccg agccgccaga
     1261 aagctctcga cctgcgcgcg ggtaggcagg cccgcccggg agccgattcg ggtgcacttc
     1321 atggccgcaa ccgcgcacgc aaaccgtgcg caggcctcga tcggctgtcc ctccacaaga
     1381 gcaagtgtga atgcgccgtg gaaggcatcc ccggctccgg tggtgtcgac cacgtccacc
     1441 ttgaagcccg gctggtgtcc ttccccggac gcttccatcc atcggtagcc atcggcgccc
     1501 agcgtgacgc cggcgtgctt cgggccgaat gccagcacac gcttcaattt ctcaccaatc
     1561 tcgacctccg gaatgaagtc ttctagagcg ggcgcggaaa atatcgcgta atccgtcagc
     1621 tccagcacct cggccagtgc ctcacgggcg ccgagatcgg cgtcgagcac tgtgggcacg
     1681 ccctccttcc gcgcgcgggc gaaggacacg cgtagtccct caagccagcg cacatcgccc
     1741 agcaccgccc cagcgccttt gatgcgctcc agcggtagcc agctcgtgcc ggacggcatg
     1801 ttgatgtcgc gcgcgcccac aatgagccgc tcgccggcat catcgacgat aatcgcggag
     1861 gtcgaggtgc gcccttcctc gaacgcctgc acggaacgga cgtcgacgcc aaggcttctg
     1921 agcccagccc ggatctttac gccagcatcg tcgtcaccga cgcggctcca aagctcagcc
     1981 ttgccaccga gacgcgcgat cgttgctgca gcgttagccg ccattccgcc gccgctctcg
     2041 acgtgctcca gggcacgaac ctttgacggg actttgggga aagcttcgat tcggtagatg
     2101 taatcgagcg cggcgtggcc gatgcagatg atccgcttca tgatttcatt tccagcatcg
     2161 accggatcgc gtttcccggt ccttcaacag aggtttttct cgaataaacg cattgtccaa
     2221 gagaataagg taagggcaag taatcgagca cgaaaacctg tgtcttattg tttttttacc
     2281 gcgcggtcga tctccatcac ctccgaaagg agcgattcga gctggcgaag tggaaccata
     2341 tttggtccgt ccgagggcgc tttgtcgggg tcctggtgag tttcaatgaa aagtccggcg
     2401 atgcccacgg caacagccgc gcgggccaat accggcacaa aacgccggtc cccgcctgag
     2461 gacgtgccct ggccacccgg ctgctggacc gagtgggtcg catcgaaaat gactggagcg
     2521 cccgtctcgg ccatgatcgg cagcgatcgc atatcgacca ccaaagtgtt gtaaccaaag
     2581 ctcacgccgc gctcggttac gagaaccttt gtgttgccgc ttcccgtaac tttggcgacg
     2641 acattcttca tgtcccaggg cgcgaggaac tggcccttct tgattttcac cacccggcca
     2701 gtctttgccg cggccaccag caggtccgtt tggcggcaga gaaaggcggg gatttgcagt
     2761 acatccacaa cctcggccac tggcgcgcac tgtgccggct cgtgcacgtc ggtcacgcag
     2821 ggcaatccga gactctcgcg aatttcggcg aaaaccggca gcgccgcagc cagtcctatg
     2881 ccgcgcttcg ctgacaaact cgtgcggttg gccttatcga acgacgtttt gtaaacgagc
     2941 ccaattccga gccggccaca gatttccttc agggccgcag ccatttcgag ggcatgcgcg
     3001 cggctttcca tctggcaagg gccagcgaaa agggcgattg gccggttgtt ggcaaacgta
     3061 acggatccga ccgtgacggt ggaat
//
LOCUS       DASUMG010000144         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_902242, whole genome shotgun sequence.
ACCESSION   DASUMG010000144 DASUMG010000000
VERSION     DASUMG010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902242"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1051)
                     /gene="cobW"
                     /locus_tag="VFX46_02340"
     CDS             complement(8..1051)
                     /gene="cobW"
                     /locus_tag="VFX46_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HEX5599454.1"
                     /translation="MAQRIPCTIITGFLGAGKTTLIRSLLEKPSGRRLALIINEFGDI
                     GVDGELLKSCGVPGCSEEDIVELANGCICCTVADDFLPTLQSLLWRPAPPDHIVIETS
                     GLALPQPLLRAFSWPEVRTRVTVDGVVTVVDGRAVADGRFAADEDAVARQRAEDESLD
                     HESPLEELFEDQLSCADLIVLSKADLLTPEELAQVEARIAQDKRKGVAVVRAANGNLP
                     VGVLLGIGASAEDDAHNRRSHHDDEDHGAEDHNDFESFSVGIGSVEGPDSISARIRQV
                     LSNGDVLRVKGFLSVSGKPMRLVVQGVGQRLETYFDRPWRPDEARTGKVVVIGLKGLD
                     RARISSVLVGAPS"
     gene            complement(1189..1857)
                     /locus_tag="VFX46_02345"
     CDS             complement(1189..1857)
                     /locus_tag="VFX46_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5599455.1"
                     /translation="MGDTMAISSPHVARVAGWALLALCATTMPALAHHAMGGEVPQTF
                     MQGLLSGLGHPVIGIDHFAFIVAVGVAAALAGQLWTLTPVFAIGAFSGCLIHLGGVTL
                     PMAELVIAASVLLLGAIIASNRDVSPFALGILFAGAGLFHGWAYGESIFGAEPTPLIA
                     YLAGFTLIQLVIAISAGFASRWMIESDGKTRTNVRLAGAVVAGIGLALFVGHIEGLLF
                     PNVQ"
     regulatory      complement(1939..2148)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2188..2730
                     /gene="cobU"
                     /locus_tag="VFX46_02350"
     CDS             2188..2730
                     /gene="cobU"
                     /locus_tag="VFX46_02350"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HEX5599456.1"
                     /translation="MANEGEYRQAVGPRWVTLVLGGARSGKTRRALDLASPFARKVYV
                     ATAEPHDEEMRARIARHVAERGPTWRTVEAPLELAKAFADTHDAEIFVVDCLTLWLSN
                     LMHAGRDIEAETPKLLAALETCPAHVVLVSNEVGLGIVPDNALARVFRDAQGRLNQEI
                     ARIADVVELVAAGLPLRLKG"
     gene            2835..>3177
                     /locus_tag="VFX46_02355"
     CDS             2835..>3177
                     /locus_tag="VFX46_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599457.1"
                     /translation="MQRLAGKVALIVGAGSVGPGWGNGRAISVLFAREGAKVFGADKS
                     IDAAAETKRLAEAEGHTIAIGEVDVTNPESIKAMVDACLQTYGRIDVLVNNVGGSAPG
                     DVVSMPLEVWDR"
BASE COUNT          672 a          990 c          969 g          546 t
ORIGIN      
        1 ccgatgctca tgatggagcg ccgaccaaga cggaggaaat acgggcgcgg tccagcccct
       61 tgagcccgat cactacgacc ttgccggtgc gcgcctcatc cggacgccag ggccgatcga
      121 aataggtttc cagccgctgc ccgactccct gcaccacaag gcgcatcggt ttgccagaca
      181 cagacaggaa gcccttcact cgaagcacgt ccccattcga tagaacctgc cggatgcgcg
      241 cggaaatgct gtccggcccc tcgacactgc cgatgcccac agaaaagctt tcgaaatcgt
      301 tgtgatcctc cgcgccgtga tcttcatcat cgtgatgtga gcggcggttg tgcgcgtcgt
      361 cctctgcgga ggcaccgata ccgagaagga cgccaaccgg caaattgccg ttggccgcgc
      421 ggacgacggc aacccccttc cgcttatctt gcgcgatcct tgcttcgacc tgcgccagct
      481 cttctggcgt aagcagatca gccttgctca gaacgatgag atccgcgcag gaaagctgat
      541 cctcgaacag ctcctcaagc gggctttcgt gatcgaggct ttcatcctcg gcgcgctgcc
      601 gggcaacggc gtcttcatcg gcagcaaagc ggccatcggc cacggcgcgg ccatcgacga
      661 cagtcaccac gccgtcaaca gtgacccgcg ttctgacctc aggccatgaa aaagcgcgca
      721 acaggggctg tggcagcgcc agcccagacg tctcgatgac gatatggtcc ggaggtgcag
      781 gacgccaaag cagcgattgc agcgtcggaa gaaaatcatc ggcgacggtg caacaaatgc
      841 agccgttcgc aagctctacg atgtcttcct ccgagcagcc cggcacgccg catgatttca
      901 gcagctcgcc atccacgccg atgtcgccga actcgttgat gatcaatgcc agccgccggc
      961 cactgggctt ttcgagcagc gagcggatca gcgttgtttt cccggccccc agaaagccgg
     1021 tgatgatggt gcagggtatc cgctgggcca tcgcgaattc cgtctcccac gtatggtcaa
     1081 cgtgtgatgg tcggctgaaa acagcctcat cgagcgcgcg attgagctgt caagagtacg
     1141 tgctcgcgct ccgccggagc agagcacccc tcttgcatcc gcgcgtcttc attggacgtt
     1201 cgggaacagc aggccttcga tatggccgac gaaaagggcc agaccgattc ccgctacgac
     1261 ggcaccggca aggcggacat tcgttcgcgt tttgccgtca ctctcaatca tccaacggct
     1321 ggcgaaaccg gcagaaatcg cgataaccag ctggatcagc gtgaaccccg cgagataagc
     1381 gatcagcggc gtgggttcag caccgaaaat cgactcgcca taggcccagc catggaaaag
     1441 cccggcgcca gcgaaaagaa ttccaagagc aaagggagaa acatcgcggt tgctggcgat
     1501 aatcgcgccg agcagaagca cagaggccgc aatcaccagc tctgccatcg gcaatgtgac
     1561 gccgccaagg tggatgaggc agcctgaaaa agcgccgatc gcgaatacag gcgtcaaggt
     1621 ccacagctgg ccggcgagcg ctgcggccac accgacagcc acgatgaacg cgaaatgatc
     1681 gatcccgatc acaggatggc caaggccgga gagcaaaccc tgcataaacg tttgcggtac
     1741 ctctccaccc atcgcatggt gggctaaggc gggcatcgtg gtcgcgcaca aggcgagcaa
     1801 tgcccatcca gcgacacgcg ccacatgagg tgaggaaatc gccatggtat cccccattca
     1861 aaatggcggg gggaatcgag actggacgcc gatcgcagta cgatcgcgcg ccaatcgcga
     1921 ccggatcacc ccgtccgttc acgctccttc tccgacggca ggtctcctgg ctcgcgaatc
     1981 gacactgcat ccaccttccc agctgatttg gtcagccagt ggcctaccgg atgcaagctc
     2041 ctcgctcaca gtcgcggggg cggccgcggc ttcgactccc gatttgggtc agtcgtcccg
     2101 cgttcccttt taatctccgg atggagaacc gtcgggcgaa acggtaatcg gagccaggcg
     2161 gagcgtcaat cgcattgagg tctgacgatg gcgaacgaag gtgaatatcg gcaggcggtc
     2221 ggaccgcgat gggtgaccct cgttctcggc ggcgcacgat ccggcaagac acgccgtgcg
     2281 cttgacttgg cttcgccatt tgcacggaaa gtctacgttg caacggccga gccgcacgac
     2341 gaggaaatgc gggcgcggat cgcgcggcac gttgcggagc ggggaccaac ctggagaacc
     2401 gtcgaagcgc cacttgagct ggcaaaggca tttgctgaca cccacgacgc agagattttt
     2461 gtcgtcgatt gcctcacgct gtggctgtcg aacctcatgc acgccggccg cgatatcgag
     2521 gcggaaaccc caaaattgtt ggcggctctc gaaacttgcc cagctcacgt cgtgctggtc
     2581 tcgaacgagg tcgggctggg catcgtaccg gacaacgcgc tggcccgcgt cttccgcgac
     2641 gcccaaggcc ggctcaatca ggagattgct cgcattgccg acgtggttga attggtggca
     2701 gcggggctgc cgctgcggct aaagggatag gcgagtagac gaagcttccc cgagggctag
     2761 agttcgttcg aaaccgcctt ctgaacccgt caaattgggc agctctcaca aatcttgaac
     2821 aatgggagaa atacatgcag cggcttgcag gcaaggttgc gctcatcgtt ggtgcaggct
     2881 cggtaggccc agggtgggga aacgggcgcg ccatctccgt tcttttcgcc cgcgaaggcg
     2941 cgaaggtgtt cggcgctgac aagagcatcg atgcagctgc cgagacgaag cggctcgcgg
     3001 aggcggaagg ccacaccatc gcgatcggcg aggtcgatgt caccaatcca gagtcgatca
     3061 aggccatggt cgatgcctgc ttgcaaacct acggccgcat cgacgtgctg gtgaacaatg
     3121 tcggcggctc cgcgcccggc gatgtcgtca gcatgccgct ggaggtgtgg gatcgcc
//
LOCUS       DASUMG010000145         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_500619, whole genome shotgun sequence.
ACCESSION   DASUMG010000145 DASUMG010000000
VERSION     DASUMG010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500619"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1084
                     /locus_tag="VFX46_02360"
     CDS             110..1084
                     /locus_tag="VFX46_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5599458.1"
                     /translation="MTKPALLMTGRMMKLIEEQLDEAFTVHRLAQPADLDETLAKVGD
                     SIEAICTGGHTGVKVDDALMAKLPKLRIIGNFGVGYDSVDAAAAGKRGIIVTNTPDVL
                     TEEVADTTLGLLLMTVRELSQAERWLREGKWATEGDYPLTQASLRDRSVGILGMGRIG
                     MAIARRLEAFAVPVSYCARHRRENLPYPYYPTAVELAKAVDTLIVITPGGPETRNIVD
                     AAVLEALGPRGILINMARGSCVDEQALINALRNRTIHAAGLDVFLNEPRINPEFLTLP
                     NAVLLPHVGSASVHTRNKMGQLVVDNLKAYLERKPPLTPVPETPFKGW"
     gene            1375..>2527
                     /locus_tag="VFX46_02365"
     CDS             1375..>2527
                     /locus_tag="VFX46_02365"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX5599459.1"
                     /translation="MGFFAESLNRIQPSATIAVSTKARQLKAQGRDIISLSAGEPDFD
                     TPINIKEAAIRAMQEGKTKYTDVDGIPELKEAIVAKFKRENGLEYKTSQISVGTGGKQ
                     VLYNALMATLNPGDEVVIPAPCWVSYADIVLLGGGKPVFAPCRIENGYRLQPEDLEKA
                     ITPRTKWFIFNAPCNPTGAAYSRDQIKALTDVLMRHPHVWVLSDDMYEHLLYDGHKFW
                     TPAQVEPGLYERTLTLNGLSKAYCMTGWRLGYAGGPEPLIKAMCKLQSQSTSNPCSIT
                     QWAAVEALTGPQDFIERNNKVFMQRRDLVVSMLNQARGIKCPKPEGAFYVYPSCEELI
                     GKKTPKGVTIKNDEDFVTALLDEEGVACVHGAAFAMSPYFRISYATSTDA"
BASE COUNT          511 a          775 c          792 g          449 t
ORIGIN      
        1 tgtcacgcgc cagggctgag ttggcaaggc ggcacagtca ttgaaccact ctcgaccgaa
       61 tgttacgacg caggcacgaa ggaaagattg ccgctggaga gagagtacga tgaccaagcc
      121 tgctctgctg atgaccggcc gaatgatgaa gctgattgag gagcaactcg atgaggcctt
      181 caccgtgcac cggttggctc agccggcgga cttggacgag acgctcgcaa aggtaggcga
      241 cagcatcgaa gcgatctgca cgggtggcca cacgggcgtg aaggttgatg acgcgctgat
      301 ggcaaagctg ccgaagctca ggatcatcgg caatttcggc gtcggttacg acagcgtgga
      361 tgcggcggcg gcgggcaagc gcggcatcat cgtcaccaat acgcccgacg tgctgacgga
      421 ggaggtggcc gataccacgc tcgggttgct ccttatgacc gtgcgcgagc tgtcccaggc
      481 ggagcgctgg ctgagggaag gcaagtgggc caccgaaggc gattacccgc tcacacaagc
      541 cagcttgcgc gaccggagcg tcggcatcct tggcatgggc cgcatcggca tggcgattgc
      601 ccggcgtctg gaagcgttcg ccgtgccggt ctcctactgc gctcggcacc ggcgggagaa
      661 cctgccctat ccctactatc cgactgctgt ggagctggcg aaggcggtcg atacgctcat
      721 tgtcatcacg cccggcggcc cggagacaag gaacatcgtc gatgccgccg tgctggaggc
      781 gctcggcccc cgtggaatcc tcatcaacat ggcacgcggt tcatgcgtcg acgagcaagc
      841 gctcatcaac gcactccgga accgcacgat tcatgcggcg gggctcgacg tgttcctgaa
      901 cgagccgagg atcaaccccg agtttctcac gctgccaaat gcagtgctgc tgccgcacgt
      961 cggctcggcc tcggttcaca cgcgcaacaa gatgggccag ctcgtcgtcg acaacctgaa
     1021 agcttatctc gagcgaaagc cgcccctgac cccggtgccg gagacacctt tcaaggggtg
     1081 gtgaccggcg caggggtgac gaaaactacg gcaaatcgag gctgcaacac agcaacgcac
     1141 ttgccccggc ctcggcctgc gcctagtagt gtcaccccgg attgaagcgg gctgggtcgc
     1201 atatccatcc gaattccggc ggatggagcg gactggttcc cgccgtcgcc gagcttcgcg
     1261 tcctcaacca gttccttgca gaaggaggat gtgcctcggc gattgtttca atctatatcg
     1321 gaggatgatg attctgcatc gcaacgcgat gcatcctatg aacgtgaggc agccatgggc
     1381 tttttcgctg agagcctgaa ccgcattcaa ccttccgcaa ccatcgcggt ctcgaccaag
     1441 gcgcgtcagc tcaaagccca gggccgcgat atcatctcgc tctcggccgg cgagccggac
     1501 ttcgacaccc ccatcaacat caaggaagcc gccattcgtg cgatgcagga gggtaagacg
     1561 aagtacacgg atgtcgacgg cattcctgag ttgaaggagg cgatcgtcgc caagttcaag
     1621 cgagaaaacg gccttgagta caagacctcg cagatatccg tcggcactgg cggcaagcag
     1681 gtgctctaca atgccttgat ggcgacgctc aatccggggg acgaggttgt cattccggcg
     1741 ccctgctggg tgtcctatgc cgatatcgtg ttgctcggcg gcgggaagcc ggtatttgca
     1801 ccctgccgca tcgagaatgg ctataggctt cagccggaag acctggagaa ggcgatcacg
     1861 ccgcggacca agtggttcat cttcaacgcg ccctgcaatc cgaccggcgc ggcctactcg
     1921 agggaccaga tcaaggcgct cacggacgtt ttgatgcggc acccccacgt ctgggtgctg
     1981 tccgacgaca tgtacgagca tctcctgtac gacggccata agttctggac gcccgcccag
     2041 gtcgagccgg gtctctacga gcgcacgctc accttgaacg ggctctccaa ggcctactgc
     2101 atgaccggct ggcgactggg ctacgcaggc gggccggagc cgctcatcaa ggcgatgtgc
     2161 aagttgcagt cgcagtcgac ctcgaacccc tgctccatca cgcaatgggc ggctgtggag
     2221 gcgctgaccg gcccgcagga cttcatcgag cgcaataaca aggtgttcat gcagcggcgg
     2281 gaccttgtgg tgtcgatgct caatcaggcg cgcggcatca agtgcccgaa gccggaaggc
     2341 gcgttctacg tctatccgtc ttgcgaggag ctgatcggca agaagacccc aaagggcgtc
     2401 accatcaaga acgacgagga cttcgtcacg gcgctcctcg atgaggaagg tgtagcctgc
     2461 gtccatggcg ccgcgtttgc catgtcgccc tatttccgca tctcctatgc gacgtcgacg
     2521 gacgcct
//
LOCUS       DASUMG010000146         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_821811, whole genome shotgun sequence.
ACCESSION   DASUMG010000146 DASUMG010000000
VERSION     DASUMG010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821811"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VFX46_02370"
     CDS             <1..1464
                     /locus_tag="VFX46_02370"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5599460.1"
                     /translation="VLARSRHLRTGDIRAFGDLARDAAGGANGPGGYFALLDRTGRHL
                     VNTRLPISAPLPRTINPDALRHVLATGRAAVGDLEISPTTRDYMSVTRVPVVIDGEVR
                     YVLAFAPHQAYINDVLRDVDWPQQWLASIVDGQGRIIARSNDNRIGIQSVWDAKRRAL
                     GLTGAGWLEGTDREGRDCVAAFQASQLSNWYVYVWVPRDVLMAPERAVRWPLLGGLAA
                     ALLTSMLAAFVAGRSVQSAIGRVLQVARGLGQGQPVSFDNTHMQEANEVGVALVKAAR
                     QIAAREEHARLLLRELSHRSKNLLAIIEVMARQSERASRSRAEFLERFGARIASLSRS
                     HDLLVSQNWQGVPITELIRTQLTPFADPVEQRIRMDGPDLVLRPEASQNIGMALHELG
                     TNASKHGALSVPDGYVRLSWKEFHDSAGNARLEMSWQEIGGPPVVMPSGKGFGFAMLD
                     SIVPSALGGLAHLDWREDGLVWTLNVPAESAVVASAG"
     gene            complement(1678..1920)
                     /locus_tag="VFX46_02375"
     CDS             complement(1678..1920)
                     /locus_tag="VFX46_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599461.1"
                     /translation="MTARSLIVLACLWIAGVSYVAGINWPVFPLDLPAADPEVRAVYD
                     RAIWAHVVQYSLIALIPAAALLGVSRAVSRRRKNAP"
     gene            complement(1930..2388)
                     /locus_tag="VFX46_02380"
     CDS             complement(1930..2388)
                     /locus_tag="VFX46_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX5599462.1"
                     /translation="MRVDSSTLLEGAYASWWARDLEAVLACFADDVVFVNHLPTDVVP
                     FAGASYGKAELRQQLQIILDQFDFLEYRPVQIVAKRRLCHSQVHFRYRHKATGLTYEG
                     RLRHIWRVEGDKIVRFEEYHDPERTRAFFELLALYDKQSQFVDRATGSRE"
     gene            complement(2436..>3082)
                     /locus_tag="VFX46_02385"
     CDS             complement(2436..>3082)
                     /locus_tag="VFX46_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEX5599463.1"
                     /translation="MASIYERDHVTVDIGLAEEGQVYVGHNLEAVIADLEKRMREAAA
                     DLEFEEAARLRDEIKRLRETELAIADDPLARQADIEERAGRYGGERKFGQAPTSSTKG
                     SPSGSRIAREYEPVQPTFAQSTSRPHKPSLDDMGPGTDRPVPARSPKVDPRMRAGAFG
                     ETIRGPHKPTLDEMGPHAERGLPIPKPAKVIDMPDEGERKRRRGRPRKTGRPGQ"
BASE COUNT          558 a          926 c          974 g          624 t
ORIGIN      
        1 gtactggcca ggtcgcgcca tctgcggacg ggcgatataa gagccttcgg ggacctcgcg
       61 cgcgatgccg ccgggggtgc aaacgggccg ggcggctatt tcgcccttct cgatcgcacg
      121 ggacggcatc tcgtcaatac gcggctgccg atcagtgcgc ccctgccaag aacgatcaac
      181 ccggacgcgc tccgccacgt cctcgcaaca ggtcgcgctg ccgtcggtga tctggagatc
      241 agcccgacaa ctcgggacta catgtctgtc acgcgggtac cggtcgtcat tgacggcgag
      301 gtgcgatacg tgctggcctt cgcgccgcac caggcttaca tcaacgatgt tttgcgggac
      361 gtcgattggc cgcagcaatg gctcgcctct attgtggatg ggcaggggcg tattatcgcc
      421 cggtcgaatg ataatcgcat cggcattcag tcagtttggg acgcgaaacg gcgggcgtta
      481 ggcctcacag gtgctggctg gctggaggga acagaccggg aagggcgcga ctgcgtcgcc
      541 gcctttcagg cttcgcaact cagcaactgg tatgtctacg tttgggtgcc gcgggatgtg
      601 ctcatggccc cggagcgtgc cgtgcgctgg ccccttttgg gtggcctagc cgccgcgttg
      661 ctcacctcga tgttggcggc gtttgtcgct gggcgctccg ttcaaagcgc catcggccgc
      721 gtgcttcaag tcgcgcgggg attggggcag gggcaaccag tcagcttcga taacacgcac
      781 atgcaggagg cgaacgaagt cggtgtggcg ctcgtcaaag ccgctcgcca aattgcggcg
      841 cgggaagaac acgcgcgctt gctcctgcgc gagctgtcgc atcgcagcaa gaatctcctt
      901 gcaattatcg aggtcatggc ccggcaatcg gagcgcgcat ccaggagcag agcggaattc
      961 cttgagcgct ttggagcgcg cattgccagc ctgtcacggt cgcatgatct gctcgtgagc
     1021 cagaactggc agggcgtccc aatcactgaa ctgatccgga cgcaattgac gcccttcgcg
     1081 gaccctgtcg agcagcggat cagaatggac ggtcctgatc ttgtacttcg gccggaagca
     1141 tctcagaaca tcggcatggc gctacacgag ctgggcacga acgccagcaa gcatggtgcg
     1201 ctctcggtac ctgatggtta cgtccggcta agttggaagg agtttcacga ttcggctggc
     1261 aacgcccgcc ttgagatgag ctggcaggaa attggcggcc cccctgttgt gatgccgtcg
     1321 ggcaagggtt ttggattcgc gatgctggat tctatcgtgc ccagtgccct cggcgggctg
     1381 gctcatcttg actggaggga ggacggcctg gtttggacac tcaacgttcc cgcagagtcc
     1441 gcggtcgttg ccagtgctgg ctaggttgag cagtccttaa ccgcgacgcc gtccaagacg
     1501 aagcccggcc cttaggcccg ggaaatgatc gcggacttcg tgattacacc aagtatttcc
     1561 gacatttggt ctctccagaa ttcgaagccg ttggattgat tgtctggctg acggaggtcg
     1621 cgccgagggg caagcggctc cacgttctcg accgagtcag caaggcgcgc gcaatcctca
     1681 aggagcattc ttcctgcgcc ttgatacggc tcgagagacg cctaagaggg cagcggcggg
     1741 aatcagagca atgagtgaat actggaccac atgcgcccaa atcgcgcggt cataaactgc
     1801 ccgcacctcc gggtctgctg ctggcaaatc gagcgggaac acgggccagt tgatgccggc
     1861 gacgtaggat acgccagcga tccagaggca ggcgagcacg attaagctac gagcggtcat
     1921 cgatgatcct cattcgcgtg acccggtcgc gcggtcaacg aactggcttt gtttgtcata
     1981 aagggccaac agctcgaaaa atgcgcgtgt tcgctcaggg tcgtggtact cctcgaagcg
     2041 cacgatcttg tcgccttcaa ctcgccaaat gtgtcgcagc cggccttcgt aagtcagccc
     2101 tgtcgcttta tgccgatagc gaaaatgaac ttgtgagtga cataatctgc gcttcgctac
     2161 gatctgcacc ggccggtact caaggaaatc gaactgatcc aaaatgatct gaagctgctg
     2221 tcgcagctcg gcctttccgt aggatgcgcc tgcgaacgga acgacgtcgg tcggcaaatg
     2281 attgacgaac acaacgtcat ctgcaaagca ggcgagcact gcctcgagat cgcgtgccca
     2341 ccacgacgcg tatgcgccct ccagaagggt actcgagtcg actcgcatgg ccataaccct
     2401 ccccatgcga gcatagctgc caatctgcga gctgactact gccccggccg gcctgttttc
     2461 cgtggcctgc cgcggcggcg cttgcgttcg ccttcatcgg gcatgtctat gaccttggcg
     2521 ggcttcggga tgggtaatcc gcgctccgcg tgtgggccca tttcgtcgag ggtgggcttg
     2581 tgcgggcccc gaatggtctc gccgaaggcg ccagcacgca tccgcggatc gacctttggg
     2641 ctgcgggctg gaacaggccg atccgtccct ggccccatgt cgtcgagcga cggtttgtga
     2701 gggcgtgatg tcgactgcgc gaaggttggc tggacgggct cgtattctcg cgcgatccgc
     2761 gatccggacg ggcttccctt agtggagctc gtgggcgcct ggccgaactt ccgttcgcct
     2821 ccgtatcgtc cagcgcgctc ctcgatgtcg gcttgccgag ccaatgggtc gtcggcaatg
     2881 gccagctcag tctcgcgaag ccgtttgatc tcgtcccgca ggcgtgccgc ttcctcgaac
     2941 tcgaggtcgg ccgcagcctc gcgcatgcgc ttttcgaggt ctgcgatgac ggcctccaaa
     3001 ttgtgcccaa cgtagacctg gccttcttcg gctagtccaa tatccacggt cacgtgatcg
     3061 cgctcgtaga tagaggccat ta
//
LOCUS       DASUMG010000147         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_500812, whole genome shotgun sequence.
ACCESSION   DASUMG010000147 DASUMG010000000
VERSION     DASUMG010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500812"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFX46_02390"
     CDS             <1..780
                     /locus_tag="VFX46_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599464.1"
                     /translation="VLDLALEAITLQKTDYNERLQSSLLAFGGCLTLASGLALMTLSP
                     WATVFFAANAIFQGGYLLWAWKALPPESEAERKGRQQTINAFIIYLAAFAFVAHAHSQ
                     GVFSHWPINAGNFTWLVELMVIVAVSAGLWAYWFFHIPRKSPSSTWTPTEDAETDIWD
                     HLPDPPWPERLRLAPEYGCWPTWDDETGLNVNPADLGLSDNLLTRLKQWDDALQATYN
                     MDDPEASRFADDDAEQRWRDVGLAIATELEREWPGPVVVRL"
     gene            complement(795..2222)
                     /locus_tag="VFX46_02395"
     CDS             complement(795..2222)
                     /locus_tag="VFX46_02395"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5599465.1"
                     /translation="MELSRFERLSDTAWQVPPTGKMRVPAIIYATEDLIRDMDDKVYT
                     QVTNVATLPGIVRASYAMPDAHWGYGFPIGGVAAFDPDEGGVVSAGGVGFDISCGVRT
                     HLTGLTRTDIMKVQGELADALYATVPVGVGSTGKITLDDDEMTAMLAGGARWAVERGY
                     GRPEDLERIEENGRQDDAKPSAVSAQARKRQRREMGTLGSGNHYLEVQEVVEIFDADT
                     AAAFGLKQGECVVTIHCGSRGLGHQIGTEFLREMAIAAETYGIALPDRELACAPIKSE
                     VGQRYLGAMRSAINCALANRQIIGHLARQVFKRMFGIDLPLMFDVSHNTCKVEPHAVG
                     SVARHLYVHRKGATRAFGPGHPSLPEALRPWGQPVLIGGSMGTGSYILVGTRESEELA
                     FSSSCHGAGRQMSRHQAVRTWSGRQVVDELKARGIEVRSPSLRGVAEEAPGAYKDVSA
                     VVDAAEEAGLSRKVARVAPLIVIKG"
BASE COUNT          398 a          744 c          713 g          425 t
ORIGIN      
        1 gttctggacc tggcgctcga agccatcacg ctccagaaga ccgactataa tgagaggcta
       61 cagtcgagcc tgctcgcgtt cggcggatgc ttgaccttgg caagcgggct cgcgctgatg
      121 acgcttagtc cttgggcaac agtgtttttt gccgcgaacg caattttcca gggcggttat
      181 ctgctctggg cgtggaaggc cctgccgccg gaatcggagg ccgagcggaa aggccgccaa
      241 cagaccatca acgctttcat tatctatctg gcggcgttcg ctttcgtcgc acacgcccat
      301 tcccagggcg tgttctcgca ctggcccata aatgctggca atttcacatg gctggtggag
      361 cttatggtga tcgtcgcggt ttcagccggc ctctgggcat actggttttt tcacataccc
      421 cgtaagagtc catcctcgac ttggactccc acggaggatg cagaaaccga catttgggac
      481 cacctccccg atcctccgtg gcccgagcgc ttgcgcctcg cgcccgagta tggctgctgg
      541 cccacgtggg acgacgagac agggctcaac gttaacccgg cagatcttgg gctgtcggat
      601 aatctactga cgcgcctgaa gcagtgggac gacgccttgc aggcaacgta caatatggat
      661 gatccggagg cctcaagatt tgccgacgat gatgccgagc agcgctggcg cgacgtgggc
      721 ttggcaatcg ccaccgaact cgagcgggag tggccggggc cagtcgtcgt gaggctctga
      781 gcttgtcctg tgcatcaccc cttgatgacg atcagcggcg ccacccgcgc caccttgcgg
      841 gacaatcccg cttcttcggc ggcatcgacg acggcgctga catccttgta agcaccgggc
      901 gcttcttcgg ccacaccgcg cagcgaaggg ctgcggacct cgatgcccct ggccttcaac
      961 tcgtccacga cctgccgacc gctccaggtg cgcaccgcct ggtgccggct catctggcgg
     1021 cctgcgccgt ggcaggatga ggagaaggca agctcctcgc tctcgcgcgt gccgacaaga
     1081 atgtacgacc ccgtgcccat gctgccgccg atcagcaccg gctgccccca cgggcgcagc
     1141 gcttcgggca ggcttgggtg accggggccg aacgcgcgcg tggcgccctt acggtgcacg
     1201 tagagatggc gcgcgacaga tcccaccgcg tgcggctcaa ccttgcaggt gttgtgggag
     1261 acgtcgaaca tcaggggcag gtcgatgccg aacatgcgtt tgaatacttg gcgcgccaga
     1321 tggccgatga tctgccggtt ggcgagagca cagttgatcg ctgaccgcat ggcgccgagg
     1381 tagcgctggc ccacttccga cttgattggc gcgcaggcga gctcccggtc gggcagagcg
     1441 atgccgtaag tctcggcggc gatcgccatc tctcgcaaga actccgtccc gatttggtgg
     1501 ccaagcccgc gagatccgca gtggatggtg acgacgcatt cgccctgctt cagcccgaag
     1561 gctgcggcgg tgtcggcgtc gaaaatttcg acgacttctt gcacctcgag gtaatggttg
     1621 cccgacccca acgtgcccat ctcgcggcgc tggcgcttcc gggcctgagc ggaaacggct
     1681 gacggcttag catcgtcttg cctgccgttt tcctcaattc gctccagatc ctccggccgg
     1741 ccgtagccgc gctcgaccgc ccaacgggcg ccgccggcca gcatagcggt catctcgtcg
     1801 tcatcgaggg tgatcttgcc cgtcgagcca acgccgacgg gaacagtcgc ataaagcgca
     1861 tcggccagct cgccctgaac cttcatgatg tcggtgcgcg tgagccctgt gaggtgagtg
     1921 cgcacgccac aggagatgtc gaagccgacg ccgccggcgg acaccacgcc gccctcgtca
     1981 ggatcgaacg ccgccacgcc gccgatcggg aatccgtagc cccaatgcgc gtccggcatg
     2041 gcgtaggacg cgcgaacaat gccgggcagc gtcgcgacgt tggtgacttg cgtgtagacc
     2101 ttatcgtcca tgtcacggat caggtcctcc gtcgcgtaga tgatcgccgg cacgcgcatt
     2161 tttcccgtag gaggaacctg ccaggccgta tccgacagac gctcgaaccg agagagttcc
     2221 atcagtgggc tcctttcgct ggagctagac gtccacgacg cattgagccc gccactcgcc
//
LOCUS       DASUMG010000148         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1143130, whole genome shotgun sequence.
ACCESSION   DASUMG010000148 DASUMG010000000
VERSION     DASUMG010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143130"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VFX46_02400"
     CDS             complement(<1..421)
                     /locus_tag="VFX46_02400"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5599466.1"
                     /translation="MALSLGLLVQSLSLPQLPLTPIGPGFYPRIVLGLLALAGAALFV
                     QNVTANRAEVPAGGEVQLRPGFWRVPLLFGIVGAYVFLLPVLGYRLATVLFVLALMPA
                     IEWPTSARAWATVISISIATSLVSHLVFERYLLVLLPR"
     gene            complement(457..1407)
                     /locus_tag="VFX46_02405"
     CDS             complement(457..1407)
                     /locus_tag="VFX46_02405"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5599467.1"
                     /translation="MKIEWVRTFCAVLGVTLVTPALADFPERPIKMIVPWAAGGDTDN
                     IFRPFVPHFQKHIGQTVVIANIGGASGTVGAREARNSPPDGYTVFAVHDYIHLTYYAG
                     MADVSYKDFEPICRIAATPSVLTASAKTPWKDFKEFVEDAKKRPGEITVGATLGSTSH
                     LFPATIEKAAGIKLKYVSYDGLAPRMNAILGGHVDLTDSNLTQKGKVEAGELKFLAVA
                     TEDRLPEIPDVPTLKENGIDIVYEVVRGLLVPKGTPEDVRAKLDEACAKAAAEPEYAE
                     ALRLQGTRVAYLNAKDYGAFLDKLDADNKAIMTELGLAKQ"
     gene            1510..2322
                     /locus_tag="VFX46_02410"
     CDS             1510..2322
                     /locus_tag="VFX46_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HEX5599468.1"
                     /translation="MDPIRVAIAGLGAIGLQVARRIDAGEIEKMVLTAVSARDQAKAE
                     RAIAGLRNPPRLLPLERLAEEADVVVECVPAEHFLAAAEGPIRLGRIFMPLSVGALLS
                     HMHLVDLARETGARIVVPSGAILGLDAMRAVAEGNVHSVRLITRKPPAGLAGAPMLVE
                     KGISVDGLTEPLKVFSGTAREAAKGFPANLNVSVALSLAGIGPDRTEVEVWADPTVTH
                     NTHTILVKSDSSQLTMTIENIPSAENPRTGVITPLSVVAALRRLTSPLVVGT"
BASE COUNT          473 a          716 c          754 g          478 t
ORIGIN      
        1 cccgcggcag cagcacgagg agatatcgct cgaagaccag atgactgacg agcgatgtgg
       61 cgatggaaat tgaaatgact gttgcccagg cacgcgctga tgtcggccat tcaatggcgg
      121 gcatcaaagc caagacgaag aggaccgtgg ccagccggta gccgagcacc ggcaacaaga
      181 acacatacgc tccgacgatg ccgaacagca gcggcacgcg ccaaaagccc ggccgcaatt
      241 gcacctcgcc gccggcgggt acttcggcgc gattagctgt gacgttttgc acgaacagcg
      301 ctgctcccgc caacgcgagc aagccgagga caatgcgtgg gtaaaagccc ggacctatcg
      361 gtgtcagcgg aagctgcggg aggctcaacg actggaccag caggccaagg ctgagcgcca
      421 ccagaatgag tccgatgacg ccgtcgcggc caaccctcat tgctttgcga gcccgagctc
      481 cgtcatgatg gccttattgt ccgcgtcgag cttgtcgaga aacgcgccgt aatccttcgc
      541 attgaggtac gccacgcgcg tgccctgcaa ccttagcgct tcggcgtatt ccggctcggc
      601 ggccgccttg gcgcaggctt cgtcgagctt cgcgcgcaca tcctcgggcg tgcccttcgg
      661 caccaacaaa ccacggacga cctcgtaaac gatgtcgatc ccgttctcct tcagagttgg
      721 gacgtcaggg atctcaggca gccgatcctc ggtagcgacg gcgagaaatt tgagttcgcc
      781 cgcctcgacc ttacccttct gggtcagatt ggaatccgtg aggtcgacgt gtccgcccag
      841 aatggcattc attcttgggg cgagaccgtc ataggatacg tatttcaatt tgattccggc
      901 agctttttcg atggtcgccg ggaacaggtg actggtcgat ccgagggtcg cgccgaccgt
      961 gatctcaccg gggcgcttct tcgcatcctc gacgaattcc ttgaaatctt tccacggtgt
     1021 cttggcgctg gccgtcagca ccgacggggt ggccgcgatc cggcagatag gctcgaaatc
     1081 cttataactt acgtcggcca tgccggcgta ataggtcagg tggatgtaat cgtggacagc
     1141 aaaaaccgta tagccatccg gcggtgagtt gcgcgcttcc cgagccccga ctgttccgga
     1201 agcgccgccg atgttggcaa tcacaaccgt ctggccgatg tgcttttgaa aatgagggac
     1261 aaaaggccgg aagatgttgt ccgtatcgcc gccggcagcc cacgggacga tcatcttgat
     1321 cgggcgctca gggaaatctg caagggccgg cgtgacaagc gtgacgccga gaacagcgca
     1381 aaacgtccgc acccattcta tcttcacggc attcctccct gatttctttg tttctgtctt
     1441 ccaggtaata acaccttaga tgacgcgcta acgcaagccg ttggtacagt gggaaggagg
     1501 gtggtgggta tggatccgat cagagttgct atcgcgggac tgggcgcgat cgggctgcag
     1561 gtggcgcgtc gcatcgatgc cggtgagatc gagaagatgg tgctgacggc agtctcggcg
     1621 cgcgatcagg cgaaggcaga gcgggcgatc gccggtctcc gaaatccacc tagacttctt
     1681 ccgttggagc ggctggccga agaagccgac gtggtcgtgg agtgtgttcc tgccgagcat
     1741 tttctggcgg ctgccgaagg tccgatccgc ctcggccgca tcttcatgcc gctctccgtc
     1801 ggcgcattgt tgagccatat gcatctcgtc gacctggctc gggagacggg agcgcgaatt
     1861 gtggttccaa gcggcgcaat tctcggcctg gatgccatgc gtgcggtcgc cgagggcaac
     1921 gtccattcgg tgcgtctcat cactcgtaag ccgcctgccg ggttggctgg cgcgcctatg
     1981 ctggtcgaaa aagggatttc ggtggatggg ctgacggagc cgctgaaagt gttctctggt
     2041 accgcgcgcg aggcagcgaa gggctttcct gcgaacctca atgtgtccgt ggcgctgtcc
     2101 ctcgccggca tcggccctga ccggaccgag gtcgaggttt gggcggatcc gacggtgacg
     2161 cataatacgc atacgatact cgtgaagtcg gactcgtccc aactcacaat gacaatcgag
     2221 aatatcccgt cggctgagaa tcctcggacc ggagtgatca cgccgttgag cgtcgtggcg
     2281 gcgctacgca ggctcacgtc gccactggtc gtcggcactt gaagacggac caggtcacgt
     2341 tcagcttcag ctcggcgcca aactccgccg tataaatccg ctgtaagcat gcgtcgcatt
     2401 ctctgttgcg cgcagcctcg a
//
LOCUS       DASUMG010000149         3385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_902654, whole genome shotgun sequence.
ACCESSION   DASUMG010000149 DASUMG010000000
VERSION     DASUMG010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902654"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            405..1187
                     /locus_tag="VFX46_02415"
     CDS             405..1187
                     /locus_tag="VFX46_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014530053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX5599469.1"
                     /translation="MSTTIVLILVALAAALASPLGGLLALWRRPTTLFLSMALGFASG
                     VLLAAINFEMLPSALELSSLPTGLAGFLAGFAAVYGLDLAVHRGKLAGRKAEQRFRVR
                     RFHRRHPPQGDEVTVLAGGTSAEEVIEGLSIGVGVAVRPELGLLVALAIVIDNLSEAL
                     SIGELAWAQAKSEQRPVKRVLGWTGLIGLSLFSSTLIGYFVIGSLPGPILGFLLASGA
                     GGMLYLTITTLLPNAEEYHYQQSAALALAAGFTIILILGRLS"
     gene            1307..1465
                     /locus_tag="VFX46_02420"
     CDS             1307..1465
                     /locus_tag="VFX46_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599470.1"
                     /translation="MSILLAIVIVLGLLWYVFGGQTAPTTTATPGEGIKIDPTPPPTT
                     PIVPRAPE"
     gene            complement(1535..1783)
                     /locus_tag="VFX46_02425"
     CDS             complement(1535..1783)
                     /locus_tag="VFX46_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599471.1"
                     /translation="MTPKIVVMIIMLALPNGDSGVHVKPFPTAESCIEAANIEATDPF
                     VRSVECAELEDGILTLRFDRSGPPQNEPVTTGSADSTG"
     gene            2202..>3385
                     /gene="fumC"
                     /locus_tag="VFX46_02430"
     CDS             2202..>3385
                     /gene="fumC"
                     /locus_tag="VFX46_02430"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX5599472.1"
                     /translation="MTSPNVRIETDTFGPIEVPADKYWGAQSQRSLGNFKIGWEKQPK
                     PIIRALGIIKRAAAETNMALGRLDRKIGEAIVKAAQEVIDGKLDDHFPLVVWQTGSGT
                     QTNMNANEVIANRAIEILGGEKGSKKPVHPNDHVNMSQSSNDTYPTAMHIAAAEQIHH
                     ELLPALHHLHRALDQKAKAWSHIIKIGRTHTQDATPLTLGQEFSAFAQQIENGIKRIE
                     LTMSMLMELAQGGTAVGTGLNAPRGFAEKVCQRIAEITGLPFKSAPNKFEALAAHDAM
                     VMSHGALNTVAVSLFKIANDIRFLGSGPRSGLGELALPENEPGSSIMPGKVNPTQCEA
                     VTMVCAQVFGNHTTMTFAGSQGHFQLNVFNPVMAYNFLQSVRLLTDAARSFTDNCIVG
                     IQP"
BASE COUNT          631 a         1063 c         1047 g          644 t
ORIGIN      
        1 gcctcaggag gccgccagcg agtcgcccca gcagactcct ccgagttcag gtgcgcagat
       61 cgtccggctc gacgctttcc ggaagaagta ggctcaggta gctggcttca tcccttttcg
      121 gctggcgtgg ctgcgcgctg tcattcaacg cgcggctagc atcacccact ctgcacagct
      181 gcgggcagcc tcaagggcga ccagcattca actggccaaa actgccgcgc ggcgcctaat
      241 tccgacattc catatcaggc gagagatggc cgggacgtcg tcgaaccggc ccgtcctgtt
      301 gtcggtttcc gggatgtttc aaacgctcgc gatttaagcg ccggtgaagg ctcagcgctc
      361 gagcggttgc ggaccattcc ctagcgcttg gcaaaccggt tcgtatgtcg acgactattg
      421 tcctgatcct tgttgccttg gcagcagccc tggcttcgcc gctcggcggt ttgcttgcgc
      481 tttggaggcg gcctacgaca ctcttcctgt ccatggccct ggggttcgcg agtggcgttc
      541 tcctggcggc catcaacttt gagatgctgc cctcggccct ggagctcagc tcgttgccga
      601 cggggctcgc cggcttcctc gccgggtttg ccgccgtcta cgggctggat ctcgccgttc
      661 atcgtggcaa gctcgccggc cgaaaggccg agcaacgctt cagggttcga cgtttccaca
      721 ggcgacatcc gccccaagga gacgaggtca ccgtccttgc cgggggcacg agcgcggagg
      781 aggtcatcga gggcctgagc atcggggttg gtgtggccgt ccggcctgag ctgggcctcc
      841 tggttgccct cgccatcgtc atcgacaact tgagcgaagc actgagcatc ggcgagctcg
      901 cctgggctca ggccaagagc gagcaacggc cagtgaagcg cgtgctcggc tggacgggac
      961 tgattgggtt gtccctcttc agctccacgt tgatcggcta ctttgtcata gggtctctgc
     1021 ccgggccgat actgggcttc ctgttggcct caggggcagg cggcatgctc tacctgacca
     1081 tcacgacatt gcttccgaat gccgaggagt accactacca gcagtcagcg gcactggccc
     1141 tggcagctgg tttcacaatc atcctgatct tggggcgcct ctcctgaccc agagccgcca
     1201 ggcccacgac ggaactcctg agagttgatg acgttcgccg agtgaaccgc cttggcggag
     1261 gtcttcgatg agggaaagtg gagatctaca agcgggttgg aggctcgtga gcatccttct
     1321 ggctatcgtg attgtattgg gcctcctttg gtacgtcttc ggcggtcaaa ctgctccgac
     1381 gaccacggca actcctggcg agggcatcaa gatcgatccc accccgccgc cgacaacacc
     1441 catcgtccct cgcgctcccg agtagctcta tcggtcgagg ctatttccac tcgagctcat
     1501 gcggagggcg gcagctgttt gccgcccgaa tggcctaacc agtcgaatcc gccgagccgg
     1561 ttgtgaccgg ctcgttctga ggcggcccac tgcggtcgaa ccgcagcgtc aggatgccgt
     1621 cctcaagctc tgcgcattcg accgagcgca cgaacgggtc tgtcgcctcg atattggcag
     1681 cctcgatgca actctccgcc gttggaaacg gcttcacatg cacgccgctg tcgccgttcg
     1741 gcagcgcgag catgatgatc atgacgacga tcttcggggt cacggcgtgt cctcgaactt
     1801 ggaacagggg gacgggcggc gtcgccgccc aggctcacgc atcacttgga agtgttgccc
     1861 cgaaagctgt gacgcgaccg ctcattgcga tccggctcaa atctcctccc gcgaatgccc
     1921 caacacccac gggcgcgact ggttacctca gtactcccga gctggcgcca gaaaaatggc
     1981 agcaaaagga ggagattggg acaatcctcc gttcgaccgt gctagcggag ccaagtaggt
     2041 ttcgacatga gcactggtcg gcttggcgag cccggggacc gaatgacggg cttcggcaat
     2101 tcatggtgag gcggtccttg gaccaagaac tggagcgggc acttacgctg tgctagtgag
     2161 ccttagcttt cagtttcggc caaaggatga gcttgcctga catgacttct cctaacgtgc
     2221 gcatcgagac ggataccttc ggccctatcg aggtccctgc cgacaagtat tggggggccc
     2281 aatcccagcg gagcctcggc aacttcaaga tcggctggga gaagcagccg aagccgatca
     2341 tccgcgcgct cgggatcatc aagcgcgctg ctgctgagac gaacatggcg ctcggccgtc
     2401 tcgatcggaa gatcggtgaa gcaatcgtca aggcggccca ggaggtcatc gacggcaagc
     2461 tcgacgacca tttcccgctc gtcgtctggc agacggggtc cggcacgcaa acgaacatga
     2521 acgccaacga ggtgatcgcc aaccgagcga tcgagatcct cggtggagag aaaggctcga
     2581 agaagcccgt gcatcccaac gaccacgtca acatgagcca gtcgtcgaac gacacgtatc
     2641 cgacggcgat gcacatcgct gccgcagagc agatccatca cgagctgctg ccggcactgc
     2701 atcaccttca tcgggcgctc gaccaaaagg cgaaggcctg gtcacacatc atcaagatcg
     2761 gccgcacgca tacgcaggac gcgacgccgc tgacgctcgg ccaggagttc tcagcgttcg
     2821 ctcagcaaat cgagaacggc atcaagcgca tcgagctgac gatgtcgatg ctgatggagc
     2881 tggctcaggg tggcacggcc gtcggcacgg gcctcaacgc gccgaggggc tttgcggaga
     2941 aggtttgcca gcggattgcc gaaattaccg gcctgccgtt caagtcggcg ccgaacaagt
     3001 tcgaggccct ggcagcgcat gatgccatgg tgatgagtca cggcgccctg aacacggttg
     3061 ccgtgtcgct gttcaagatt gcgaacgaca tccgcttcct gggttcagga ccgcgctctg
     3121 gcctcggtga gctggcgttg ccggagaacg agccgggctc atctatcatg ccgggtaagg
     3181 tgaacccgac ccagtgcgag gccgtgacga tggtgtgtgc ccaggtgttc ggcaaccaca
     3241 ccacaatgac gtttgcgggc agccagggcc acttccagct caacgtcttc aacccggtga
     3301 tggcctacaa cttcctgcag tcggttcggc tgctgaccga tgcagcgcgg agcttcacgg
     3361 acaactgcat cgtcggcatc cagcc
//
LOCUS       DASUMG010000150         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1063023, whole genome shotgun sequence.
ACCESSION   DASUMG010000150 DASUMG010000000
VERSION     DASUMG010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1063023"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..720)
                     /locus_tag="VFX46_02435"
     CDS             complement(172..720)
                     /locus_tag="VFX46_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5599473.1"
                     /translation="MTVDVGGRLRYVRERYNLSQRELAKRSGVPNSTISLIEANKVNP
                     SIGALKRILDGVPIGLAEFFAIEPDKPRKIFYAAEELTEIGKGGISYRQVGENLVGRA
                     LQILKERYEPGADTGRVPLVHEGEEGGIIISGRLEVTVDSERRILGPGDAYYFESRRP
                     HRFRCIGPTPCEVVSACTPPSF"
     gene            928..>2028
                     /locus_tag="VFX46_02440"
     CDS             928..>2028
                     /locus_tag="VFX46_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5599474.1"
                     /translation="MAPFDMKPNELESYWLPFTPNRSFKKRPRLFSGAKDMYFFTPDG
                     RKVIDGSSGLFCVNAGHGREPIVKAIQEAAARLDYAPPFQYAHPQVFELSSRLALMAP
                     GDLEYTLFANSGSEAVDTALKVALAYHRARGEGSRTRFIGRERGYHGVGFGGISVGGM
                     VANRKMFGNMLTGVDHLPTTYNRAKQAFSVGEPDWGAETADELERLIGLHDASTIAAV
                     IVEPVAGSTGCLPPPKGYLQRLREITEKHGILLIFDEVITGFGRLGYAFAAERYGVVP
                     DMITFAKGVTNGAVPMSGVLVRKHIYETHMQGPEHMIELFHGYTYSGHPLAAAAGLAT
                     LNIYKDEGLFEKARANEKAFADAMMSLKGLPNV"
BASE COUNT          384 a          621 c          634 g          389 t
ORIGIN      
        1 tcgggcctcc cctgcccgca cggcgccact caacgccgat tcgactaacg actcctaaat
       61 gacgccagga ttcgggtgcg gccgggactg aagggcggcg caattagggc cgtacacagc
      121 tgagttgatg ttagcttcgg aagcggcgct ggggaacggt ttaacgtcgc ctcaaaacga
      181 cggcggcgtg caggcgctga cgacctcgca gggagtcggc ccgatgcagc ggaaacgatg
      241 gggacggcgg ctttcgaagt aataggcgtc gcccggccct agaatccgcc gctcggaatc
      301 gaccgtcacc tccagccggc cgctgataat gatgcctcct tcctcgccct cgtggacgag
      361 aggcacacgg ccggtgtcgg ccccaggttc gtagcgctct ttcaagatct gcaacgcccg
      421 cccgaccagg ttctcgccca cctgccggta ggagatgccg ccctttccga tctcggtcag
      481 ctcctcggcc gcataaaaga ttttgcgcgg cttgtccggc tcgatggcga agaattcggc
      541 gagcccaatc gggacaccgt cgaggatacg tttcagcgcc ccgatggacg ggtttacctt
      601 gttcgcttcg atcagcgaga tcgtcgagtt tggaacacca gaccgtttgg ccaactcacg
      661 ctgagaaagg ttatagcgtt cgcgaacgta gcgcagacga ccgccgacat cgactgtcat
      721 ggagctgccc catgtttcga atgttcaata tttcgaaaac tgtgccgggc gagggcagcg
      781 aaatcaaggg cctacaactt gggagaaaag ggcttgatag gctatgccga tcaggcctct
      841 actcctactc gggcgggcgt ctgcgaattt gacaactgta ccccccgagt ggctactgac
      901 gccctgagac acgattggag gattgcgatg gcgccgttcg atatgaaacc caacgagctt
      961 gagtcctatt ggctgccatt tacgccaaac cgctcgttca agaagagacc tcgcctcttc
     1021 tccggcgcca aggatatgta cttcttcacg ccggatggcc gtaaggtgat cgacggctct
     1081 tccggcctgt tctgcgtaaa tgctggccac ggccgcgagc cgatcgtgaa ggcgatccag
     1141 gaggcagcag cgcgcctcga ctatgcgccg ccgttccaat acgcccatcc gcaggtgttc
     1201 gagctctcat cccgtctcgc cctgatggcg ccgggcgacc tcgaatatac gttgttcgcg
     1261 aactccggct cggaggcagt cgatacggcg cttaaggttg cactcgctta ccatcgtgcg
     1321 cgcggcgagg ggagccggac ccgattcatt gggcgcgagc gcggctatca cggcgtcggt
     1381 tttggcggca tctccgtggg cggcatggtc gccaaccgga agatgttcgg caacatgctg
     1441 acgggcgttg accatcttcc gacgacctac aaccgggcta agcaggcgtt ttctgtcggt
     1501 gagccggatt ggggcgccga gacggcggac gagctggagc ggctgatcgg tttgcacgat
     1561 gcttcgacca tcgctgccgt gatcgtcgag ccggtggccg gttcgacggg ctgcctaccg
     1621 ccaccaaagg ggtacctgca gcgcctccgc gagatcaccg agaagcatgg catcctgctg
     1681 atcttcgacg aggtgatcac cggcttcggt cgcctcggct atgcgttcgc ggccgagcgg
     1741 tatggcgtcg ttcccgacat gatcacgttc gccaagggcg tgaccaacgg tgcggtaccg
     1801 atgtcgggcg ttctggtgcg caagcacatt tacgagaccc acatgcaagg gcccgagcac
     1861 atgatcgagc tgttccacgg ctacacctac tcgggccacc cgttggcggc tgcggccggt
     1921 ctggcgacgc tcaacatcta caaggacgag gggctgttcg agaaagcgcg ggccaatgag
     1981 aaggcctttg ccgatgccat gatgagcctc aagggcctgc cgaacgtg
//
LOCUS       DASUMG010000151         3259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_501182, whole genome shotgun sequence.
ACCESSION   DASUMG010000151 DASUMG010000000
VERSION     DASUMG010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501182"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VFX46_02445"
     CDS             complement(<1..863)
                     /locus_tag="VFX46_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599475.1"
                     /translation="MIASRLPWEKYERIDSGCAANSGAEESVWPITEPDPTSALISNP
                     QPIDPGEAIWKPLFDRAQLTKRANVVCMKWGTRYGPEWVNRLAAMVRRNTTWNIRFVC
                     FTDDPSGIRPDIECQPMPVVEFDPKLGRYWPKLGLFQENLGGLEGMTLYLDLDVVVMG
                     SLDSFFSYPGRFCIVREWKDPHLGYGNSSVVRYFIGLESAVLDRFYATSPEQIASIYA
                     CKEQNFLTRAVDEVTFWPDEWCPAFSRACLPRTRILRYFCKPSRPTSGRILVFYGSIT
                     PASAMVGEHDP"
     gene            complement(1184..1255)
                     /locus_tag="VFX46_02450"
                     /pseudo
     CDS             complement(1184..1255)
                     /locus_tag="VFX46_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
     gene            1529..3076
                     /gene="guaA"
                     /locus_tag="VFX46_02455"
     CDS             1529..3076
                     /gene="guaA"
                     /locus_tag="VFX46_02455"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX5599476.1"
                     /translation="MKDTVLILDFGSQFTQLIARRVREAGVYCEIVPYDRAEDGLQRL
                     QPKAIILSGGPASVYESDTPAAPDAVFRAGLPVLGICYGEHTMVAQLGGEVRPGTRRE
                     FGRAEIEVVNHSPLFEGVWSKGTRDHVWMSHGDHVASLPPGFRVIAVSEGAPFAAIAD
                     EERRLYGVQFHPEVAHTPRGAELLRNFVIGIAGCRGDWSMRAFREEAIARIREQVGEG
                     RVICGLSGGVDSAVAAVLIHEAIGDQLTCIFVDHGLLRQGEAEEVVTLFRGHYNIPLV
                     HVDAESRFLAALQGKSDPEEKRKTIGHLFIDVFEEEAKKLGGAEFLAQGTLYPDVIES
                     VSFTGGPSVTIKSHHNVGGLPERMKLKLVEPLRELFKDEVRALGRELGLPESFIERHP
                     FPGPGLAIRVPGEVTREKLDILRQADAIYLEEIRNAGLYKEIWQAFAVLLPVRTVGVM
                     GDARTYEYACALRAVTSTDGMTADYYPFPHEFLGRTATRIINEVKGINRVVYDITSKP
                     PGTIEWE"
BASE COUNT          639 a          931 c         1045 g          644 t
ORIGIN      
        1 ttcggatcat gctcgccgac cattgccgat gccggggtga ttgagccata gaagacgaga
       61 attcggcccg atgtcggccg gcttggcttg cagaaatagc ggagaatgcg ggtccgcggt
      121 aaacacgcgc gggagaaggc gggacaccat tcgtccggcc agaacgtcac ctcatcgacg
      181 gcgcgtgtga ggaagttctg ctctttgcaa gcatagatgc tcgcaatttg ctccggagaa
      241 gtcgcgtaga accgatcgag aactgcactt tccagtccaa tgaaatagcg aacgacggag
      301 ctgttgccat agccgaggtg cgggtccttc cattcgcgaa cgatgcagaa gcgccccgga
      361 taggaaaaga atgagtcgag gctgcccatg acgaccacgt ccaggtcgag gtaaagcgtc
      421 ataccctcaa ggccgccgag gttctcctgg aataatccga gcttcggcca ataccgcccg
      481 agctttggat cgaactcgac gaccggcata ggttggcact cgatgtccgg gcggatgccg
      541 gaggggtcat cggtgaaaca gacgaaccgg atgttccatg tggtattgcg acgaaccatc
      601 gctgcaagcc gatttaccca ttccggcccg tagcgcgtgc cccacttcat acagacgacg
      661 ttcgcgcgtt tcgtaagctg cgcgcgatcg aacaacggtt tccaaatggc ttcgcctgga
      721 tcgatcggct gagggtttga aatcagcgcg ctggtgggat cgggctctgt tatgggccag
      781 acgctctcct ccgccccgct gttggccgcg catccactgt cgatgcgctc atacttttcc
      841 cagggaagcc ggctcgcaat cattgcgcgt cacccgtgag aacattacga gtacctgcga
      901 cggcaacatt gcggggcaag cttgtggcag gctgaactga gagcgtttat cgacaagccc
      961 cctggccagc acgttcagca aattcggcgg gtcgagcagg ttatccgaat gaatttggcc
     1021 cgcaggattt agacatccga gcgatgtctt tctgaaaggc aatatccaca aaccgagttg
     1081 gagtcagagg cgaggggacg gcgatttcgg ctttcgtaat ggggccggca aacgccgaaa
     1141 gacggttcgc caacacgtca ggtgcggcaa cggggcgagc tggtcaggcc gtcatgcagc
     1201 acttcttgta cttcttgccc gacccgcagg ggcaaggatc gttgcggccg atcttggggc
     1261 tggagcgaac gaacggagca atgggccggt ggatatgatg gtggtgatgg ccgtggtggt
     1321 catggccaca gccgcacgcg tcatcatgaa cgtgatcgtg acgggccttc gttgcgtcgc
     1381 cagggttcgt tgtttgggtg ctcatcggct caggccctca ctgattgggt ttcccgcaat
     1441 atagtgcggg atggctgtcc agtagacgcc tgttagtcgc accctggcgg ctgttgcagt
     1501 cctccaggga ttgtcgtaac cgctcgtcat gaaagacact gtcctcattc tcgatttcgg
     1561 ctcccagttt acacagctca ttgcgcggcg cgtgcgcgag gccggcgtgt actgcgagat
     1621 cgttccctac gacagggccg aagacgggct ccagcggctt cagcccaaag cgataatcct
     1681 ctccggcggc cccgccagtg tgtatgaaag cgacacgcct gcggctccag atgcagtctt
     1741 ccgtgccggc ttgccggtgc ttggcatctg ctatggcgag cacacgatgg tggctcagct
     1801 cggcggcgag gtgaggccgg gcacgcgtcg ggagtttggc cgcgccgaga tcgaggtggt
     1861 taatcacagc ccgctgttcg agggcgtctg gtccaagggc acgcgggatc acgtttggat
     1921 gagccatggc gaccacgtcg ccagcctccc gccaggtttt cgagtgattg cggtctcaga
     1981 gggggcgccg ttcgcggcca ttgccgacga agagcgccgc ctctatggcg tgcagttcca
     2041 tcctgaggtg gcccatacgc cgcgaggagc cgagctgctg cgcaatttcg tgatcggaat
     2101 cgccggatgt cgtggcgact ggagcatgcg ggcgtttcgc gaggaagcga tcgcgcgcat
     2161 ccgcgagcag gtcggcgagg gcagggtcat ttgcggacta tcgggcgggg tggattctgc
     2221 cgtggccgcg gtgctgatcc acgaggccat cggcgaccag ctcacctgca ttttcgtcga
     2281 tcatggtctg ctgcggcagg gcgaggccga agaagtcgtc acgctgttcc gcggacatta
     2341 caacattccg ctggttcacg ttgatgcaga aagcaggttc ctggccgccc ttcagggcaa
     2401 gagcgaccct gaggagaaac gcaagacgat cggccatctc ttcatcgacg tgttcgagga
     2461 ggaagcgaag aagctcggcg gcgccgaatt tctcgcccag ggtaccctct atcccgacgt
     2521 gatcgagagc gtctcgttca ctggtggccc gtccgttacg atcaagtcgc accacaatgt
     2581 cggcggactg cccgaacgca tgaagctgaa gctggtggag ccgctgcgcg agctgttcaa
     2641 ggacgaggtt cgcgcattgg gccgcgagct ggggctgccc gagagcttca tcgaacggca
     2701 cccgtttcct gggccgggcc ttgccattcg cgtgccggga gaggtcactc gtgagaagct
     2761 cgacattctg cggcaggctg atgcgattta tctggaggaa atccgcaacg cgggcctcta
     2821 caaagaaatt tggcaggctt tcgcagtgct attgcccgtg cgcactgtgg gcgtcatggg
     2881 cgatgcgcgc acttacgaat atgcctgcgc tctcagggcc gtgacctcga cggacggcat
     2941 gaccgctgac tactacccct tcccgcacga gtttctcgga cgcaccgcga cgcgcatcat
     3001 caacgaggtg aaaggtatca atcgggtcgt ttacgacatc acctccaagc cgccgggaac
     3061 catcgagtgg gagtgacgta cgctcaccac gatagcggaa gttttttctt gctatcgacc
     3121 gctcatgaaa aaagcccctc agcacgaagg ctgggggctt gagagcttcg gcccggcggg
     3181 gacaccgggg atggatccgg gccgaaggga gacacatcag tagaagccct tgcaggcgaa
     3241 atacttgttc ttccagaag
//
LOCUS       DASUMG010000152         3784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_340430, whole genome shotgun sequence.
ACCESSION   DASUMG010000152 DASUMG010000000
VERSION     DASUMG010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340430"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1989)
                     /locus_tag="VFX46_02460"
     CDS             complement(<1..1989)
                     /locus_tag="VFX46_02460"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX5599477.1"
                     /translation="MGGLKDRRGKAAHFGRTLLRGPAGAPINGLLFALVALIFLASAA
                     DPAWALEPITVHPDQDRIEITGQGEIHGGQGDTLQVETAAGPDGVKGRMSIRASTPGT
                     SPNWIVFALHNPTDKVIERWLTAERYTVIGSSVIWPDLDARRIEAVTPSLGFLPDRVK
                     SDRADIFRITLEPGQTITYAAELASERVPRIYLWKPLEYELKARDRQLFNGIMLGITG
                     LLAIFLTAIFAANHKAIFPAASLVAWCVLGYLCVDFGFWHKLFQLRAEDNAVYRAATE
                     SAIAASIVIFVGVFLRLSAWHGFIRMLATIWIAAQLSLVFIAVIDPRLAASFARASFV
                     AIGAVGALLTLYLALRGQDRALALIPTWLLFLVWIFAAGVTLTGQLSGDIVVSGLVAG
                     LVLIVVLMGFTVTQFAFRSYEPVYGSAPSELQLRSLAIDGAGAAVWEWYARRDEIKVS
                     PIIEASLGLTAGELSTKVDDFVKHLHPADRERFRLVLLGVQERNGGSLHIELRMRHAD
                     NSYRWFELEAASVPNSDRRALRCVGLMRDITEAKRAQERLMHDAVHDSLTGLPNRELF
                     LDRLGVAVMRAKTEPQIRPTVFFIDIDRFKNVNASLGLIVGDSLLLTVARRLMRHIGP
                     QDTLARVGGDQFAILLVAQHEPRELAMIAERIRRALRSP"
     gene            complement(1989..2579)
                     /locus_tag="VFX46_02465"
     CDS             complement(1989..2579)
                     /locus_tag="VFX46_02465"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5599478.1"
                     /translation="MAFLRSGIGGDWLPHVRVDGIHLRPPHMGDYAAWAELRALSRDH
                     LTPWEPQWTRDELSRDAFRRRVRHYQRELREDLGYAFFLFRDLDDRLLGGITLSNVRR
                     GVTQAAAVGYWIGKPYAGRGLMTKAVAGIVRFAFEELKLHRLEAACMPSNTASIRVLE
                     KNGFQREGLARRYLKINGVWQDHLLYATLADDPREG"
     gene            complement(2596..>3784)
                     /locus_tag="VFX46_02470"
     CDS             complement(2596..>3784)
                     /locus_tag="VFX46_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5599479.1"
                     /translation="GVWVAAGARHESEHEHGISHLLEHMAFKGTRRRTARRIAEEIEQ
                     VGGELNAATSLEMTAYYARVLKGDEAIALDILADILQNSTFSEEELEREREVILQEIA
                     SIQDSPDEIAYDLVQDAAYPGQAVGRPIIGTRESVSRLTPADLRTFLRTRYLAPRMVI
                     SAAGAIDHNNLVRHAEALFGGLNGGDSHNGAAAQYVGGRRTSRKVFEQSHLLIGFESP
                     SYRDEEFYAAQVFSGLFGGGMSSRLFQAARENRGLCYAIYSTAWGMADSGMFDIHAAT
                     GPEQMGELIDVIGAELFNLASSPPTAPEVSRAKAQLKAALLTSFESSSARAEQMARQL
                     LFFDRLLPASELVERIEEVTPERIRAVAERVARSAPPSIAVVGAGRRSSDYAIRAERR
                     LAS"
BASE COUNT          745 a         1236 c         1167 g          636 t
ORIGIN      
        1 cggcgaacgc aaagcgcgcc ttatgcgctc ggcgatcatc gcgagctcgc gaggctcgtg
       61 ctgggccacc aacaggatcg cgaattgatc gccaccgacc cgcgccagcg tgtcctgcgg
      121 gccgatatgg cgcatcaggc gccgcgccac cgtgagcagc aggctgtctc cgacgatcag
      181 cccgagcgag gcgttgacgt tcttgaaccg gtcgatatcg ataaagaaaa ccgtcgggcg
      241 gatctgcggc tcggtcttgg cgcgcatgac ggccacgccg agccggtcga ggaagagctc
      301 ccgattgggc aggccggtca ggctgtcgtg aacggcatca tgcatgagcc gctcctgtgc
      361 ccgtttggcc tccgtaatgt cgcgcatcaa gccgacacac cgcagggccc gccgatccga
      421 attcggcacg cttgccgcct ctagctcgaa ccagcggtag gaattgtcgg cgtgccgcat
      481 tctgagctcg atgtggaggc tgcctccgtt gcgctcctgc acgcccaaga gcactaggcg
      541 aaaacgctcc cgatcggccg gatgtaaatg cttgacgaag tcatcgacct tcgtcgacag
      601 ctcacctgcc gtcagcccga gcgaggcctc gataatgggg ctgactttga tctcatcgcg
      661 ccgggcatac cattcccaga cagctgctcc ggcgccatcg atggcaaggg agcggagctg
      721 cagctcgctc ggggcagagc catagaccgg ctcgtaggag cggaaggcaa actgcgtaac
      781 ggtaaagccc atcagcacga cgatgaggac gagcccagcc accaggcccg acacgacaat
      841 gtcgcccgac agctgacctg tcagcgtgac gccggcagcg aagatccaga cgagaaacag
      901 gagccaagtc ggaatcagag ccaacgctcg gtcctggccg cgtaaggcca ggtacagggt
      961 cagcaaggct ccaaccgctc cgatcgcaac gaacgacgcc cgcgcaaatg aggcggccag
     1021 ccgcggatcg atgacggcga tgaaaaccag ggagagctgc gccgcgatcc agatcgtcgc
     1081 cagcatgcga atgaacccat gccaggcgct gaggcgcagg aacacgccga cgaagatgac
     1141 gatgctggcc gcgatggccg actcggttgc ggcccggtag acggcattgt cctcagctcg
     1201 aagctgaaag agcttgtgcc agaagccgaa gtcaacgcag aggtagccga gcacgcacca
     1261 cgccaccagg gacgcggccg gaaagatcgc cttgtggttg gcagcgaaaa ttgccgtcaa
     1321 gaagatcgcg agcagacccg tgatcccgag catgatgccg ttgaaaagct gccggtcgcg
     1381 ggctttgagc tcgtattcca atggcttcca gagatagatg cgcgggacgc gttcggaggc
     1441 cagctccgcg gcgtaggtga tcgtctggcc tggctccagc gtgatgcgga agatgtcggc
     1501 gcgatcgctt ttcacgcgat ccggaagaaa tccgagtgac ggcgtcacag cctcgatgcg
     1561 gcgggcgtcc agatccggcc aaatcacgga cgagccgatg accgtgtagc gctcggcggt
     1621 gagccaccgc tcaatgactt tgtctgtcgg gttgtggagt gcgaaaacaa tccagttcgg
     1681 gctcgtgccc ggtgtgctgg ccctgatcga catgcgcccc ttgacgccgt ccgggcctgc
     1741 agccgtttcg acctgaagtg tatcaccttg accgccgtga atctcacctt gaccggtgat
     1801 ctcgatccgg tcctgatccg ggtgaaccgt tataggctcc agtgcccatg cgggatcggc
     1861 agcgctggcg agaaaaatta gcgcaaccag cgcaaacagc aggccgttaa tgggcgctcc
     1921 cgccggccct cttagcaagg tccggccgaa gtgcgctgcc ttccctcttc gatctttcag
     1981 gccccccact atccctcccg cgggtcgtca gccaaagtcg cgtaaagcag gtgatcctgc
     2041 caaacgccat tgatcttgag ataccggcga gctagccctt cacgctgaaa tccgttcttc
     2101 tccagcaccc ggatcgaagc agtattgctc ggcatgcagg cagcttcgag gcgatgcaac
     2161 ttaagctcct cgaacgcaaa ccggacgatc cctgccacgg ccttcgtcat caagccccgg
     2221 ccggcatagg gcttgccgat ccaatagccc accgccgccg cctgggttac gccacgccga
     2281 acattgctca gcgtaatccc tccgagcagg cgatcgtcca aatcacggaa gaggaagaac
     2341 gcgtagccca agtcctcccg cagctcccgc tggtagtgcc gcacccggcg gcggaaggcg
     2401 tcgcgagaca gctcatcccg cgtccactga ggttcccagg gtgtgaggtg gtcgcggctc
     2461 aatgccctga gctcggccca ggcggcgtag tcgcccatgt gaggcggcct gaggtggatg
     2521 ccgtcaacgc gcacgtgtgg gagccaatcg ccgccgatgc cggatctcag gaaagccatc
     2581 gccctgctcc tttcgctagc ttgcgaggcg gcgttcggcg cgaattgcgt aatcggagga
     2641 acgcctgccg gcgcccacca cggcaatgga aggtggcgcc gaccgggcaa cgcgctcggc
     2701 gaccgcccgg atccgctcag gagtgacttc ctcgatccgc tccacgagct cggacgccgg
     2761 gagcagccga tcgaaaaaca ggagctggcg cgccatctgc tcggccctcg ccgacgaact
     2821 ctcgaagctg gtcagcagag ccgccttcag ctgcgccttg gcgcggctca cttccggcgc
     2881 ggtcggaggg gatgaggcga gattgaacag ctccgctccg atcacgtcga tcagctcccc
     2941 catctgctcc ggtcccgtcg cggcatggat gtcgaacatg cccgaatcag ccattcccca
     3001 ggccgtcgag tagatggcgt aacacagccc ccggttctca cgggcggcct gaaacagccg
     3061 ggacgacatc cctccgccga acagtccgga aaagacctgc gcggcgtaga actcctcatc
     3121 gcggtaggac ggcgactcga acccgataag caaatgactc tgctcgaaaa ccttcctgga
     3181 tgtacgccgc ccccccacgt attgcgccgc cgcaccattg tgcgaatcac cgccattcag
     3241 gccgccaaat agggcttcag cgtggcggac gaggttgttg tggtcaatcg ctcctgcggc
     3301 ggaaatcacc atcctcggcg caagatagcg cgtgcgaaga aacgttctaa ggtcggctgg
     3361 cgtgagccga gagacactct ccctcgtacc gatgatcggc cggccgacag cctggcccgg
     3421 gtatgcagca tcctgaacga ggtcataggc gatctcgtca gggctgtcct gaatactggc
     3481 gatctcctga aggatcacct ctcgctcgcg ctcgagctct tcctcggaaa aggtcgagtt
     3541 ctgcaaaatg tcggccagga tatcgagagc aatcgcctca tcacccttga gcactcgtgc
     3601 gtaataggcc gtcatttcga ggctcgtcgc ggcgttgagc tcgcccccga cctgctcgat
     3661 ttcctcggcg atccggcggg cggtgcgccg ccgggtcccc ttgaacgcca tgtgctcgag
     3721 cagatgcgag atgccgtgct cgtgctcgct ttcatggcgc gcgcctgcgg cgacccatac
     3781 gccg
//
LOCUS       DASUMG010000153         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_983223, whole genome shotgun sequence.
ACCESSION   DASUMG010000153 DASUMG010000000
VERSION     DASUMG010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983223"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFX46_02475"
     CDS             <1..461
                     /locus_tag="VFX46_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX5599480.1"
                     /translation="IYLVMTLGAFACILAMRRSDGPTEEISDLAGLAQTNLPMAFAFG
                     ALMFSMAGIPPLAGFFAKFYVFLAAINAGLYTLAVIGVLASVIAAYYYLYIVKVMFFD
                     EPKDSFLPVRSKEGFVIAVSAALMIFAFIVPYLPASLVSVADAAAASFRY"
     gene            469..1266
                     /locus_tag="VFX46_02480"
     CDS             469..1266
                     /locus_tag="VFX46_02480"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX5599481.1"
                     /translation="MPGAAPALPPGYGLLTFDEIGSTNAEAMRLGRAGETGPLWIWAR
                     MQTRGRGRSGRTWASPPGNLYTSLLIRLDCSPDAVHQLSLLAGVATYEAVRSACSRSG
                     GAVSGLRLKWPNDVLIDDAKLAGILPECTRVPGSSNAFLAVVGIGINLATHPEGLGRS
                     VTDLAAHGCLVEPADMLSELAAAMHQWLTEWDNGAGFARVREAWLERAGRPGEHITIN
                     TGQEVVEGSFLGIDPSGALVLRDPSGAQRRFTYGDVTLGAAAGANEQ"
     gene            1263..2972
                     /locus_tag="VFX46_02485"
     CDS             1263..2972
                     /locus_tag="VFX46_02485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX5599482.1"
                     /translation="MTDMWRAGSRPGSSEDEIVFLGLGGLGEIGLNVYLYGMGLEDNR
                     RWLMVDLGLTFAEEHEPGVEIVLPDLRFIEEERGALEGIVLTHAHEDHFGAVIDLWDR
                     LRAPIYATPFTAAMLRSKLAEEGSGIEPPITVVPMGARFKVGPFDLEFVTMAHSIPEP
                     SGLVIRTPHGIIFHTGDWKLDRTPLVGDPTDEEKIRSLGREGVRALICDSTNAFREGR
                     SPSEADVAESLKAIIAKAKRRVAVTTFASNVARIRAIAEAAKATRRHLVVAGRAMHRV
                     IQVAMETGYLPQDFRYLDQDQFAYLEPDEVLMLCTGSQGEPRAALSRIAEGEHPDLRL
                     GQGDLVIFSSRTIPGNEKAVTRVQNNLALLGVDILTDADALVHVTGHPRREELKEMYA
                     WLKPAVAVPMHGEARHLREHAWLAKAAGVLDVHPLRNGQMIRLAPDPVAIIDEAPVGR
                     LYRDGRLIVSGEGSVRERRRLAFAGIVAIGMAMSRRGELLSDPSIAMEGIPAETADGM
                     PMTEVVFDAIDGTLRSIPAGKRRDIELVRDAVRRSVRAAIEQVWGKKPIAKVLINVVD
                     TRR"
     gene            3123..3527
                     /gene="mce"
                     /locus_tag="VFX46_02490"
     CDS             3123..3527
                     /gene="mce"
                     /locus_tag="VFX46_02490"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007626175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX5599483.1"
                     /translation="MIGRLNHVAIAVRDLQAAAATYRETLGATVSEPLPQPDHGVTVV
                     FVTLPNTKIELLEPLGPNSPIAKFLERNADGGIHHVCYEVDDIIAARDRLRAAGARVL
                     GDGEPKIGAHGKPVLFLHPKDFFGTLIELEQA"
BASE COUNT          615 a         1121 c         1212 g          682 t
ORIGIN      
        1 ggatctacct cgtgatgacg ctcggcgcgt tcgcctgcat cctggccatg cgccgctccg
       61 acggcccgac ggaggaaatc agcgacctcg ctggcctggc gcagaccaat ctgccgatgg
      121 cctttgcctt cggcgcgctg atgttctcca tggcggggat tccgcccttg gccggcttct
      181 ttgcgaaatt ctacgtgttc cttgctgcca taaatgcggg gctttacacc ttggccgtca
      241 tcggcgtgct ggcgagcgtg atcgctgcct actactacct ctacattgtg aaggtgatgt
      301 tcttcgatga accgaaggac agcttcctgc ccgtgcggag caaggagggc ttcgtcattg
      361 ccgtatcggc agctctcatg atcttcgcgt tcatcgtgcc ctacctgccg gcgtcgctcg
      421 tctctgtcgc cgatgcggcg gcagcaagct tccggtactg aacggccgat gcccggggcc
      481 gctccagcgc ttccgcccgg ctacggcctc ctcacctttg acgagatcgg ctcgaccaac
      541 gccgaggcaa tgcgcctggg cagggccgga gaaacaggcc cactgtggat ctgggcgcgc
      601 atgcagacac gggggcgcgg ccgctcaggc cgcacatggg catcgccccc ggggaaccta
      661 tacaccagcc tgttgatccg gctcgactgc tctccggatg ctgtccacca gctctccctg
      721 cttgcggggg ttgcgactta cgaggccgtg cgttctgcct gctctcgaag tggcggagct
      781 gtcagcggcc tccgattgaa gtggcccaac gatgttctca ttgacgacgc caagctggcc
      841 ggcattttgc cggagtgcac gcgcgtcccg ggtagttcca acgccttcct ggcggtggta
      901 ggcatcggca tcaatcttgc aactcatccg gagggtttgg gccgctcagt aacggatctt
      961 gccgcgcacg ggtgcctcgt tgagcctgca gacatgctgt ccgagcttgc tgctgcaatg
     1021 catcagtggc tgaccgagtg ggataacggc gccggtttcg cgcgcgtgcg ggaggcctgg
     1081 ttggagcggg cggggaggcc gggtgagcac atcaccatca acacagggca ggaggttgtg
     1141 gaaggtagct tcctcgggat cgacccgtca ggagcactcg ttttacgtga tcccagcggt
     1201 gcccagcgcc gcttcaccta tggagatgta acgctggggg cggcagcggg cgcgaacgag
     1261 caatgacgga tatgtggcgg gcaggctccc ggccggggtc gagcgaggac gagatcgttt
     1321 ttctggggct tggaggtctc ggagagatcg ggctcaacgt ctacctctat ggcatggggc
     1381 tcgaggacaa ccgccgttgg ctgatggtcg acctcggcct cacgtttgcc gaggagcacg
     1441 agcctggcgt cgagatagtc ctgccagacc tgcgcttcat cgaggaggag cgcggcgccc
     1501 ttgagggcat tgtccttacc cacgctcacg aggaccactt tggagccgtg atcgacctgt
     1561 gggaccggct ccgcgcgccc atctacgcga cgccgttcac cgcggccatg ctgcgctcga
     1621 agctcgccga ggagggcagc ggaattgagc cgccaatcac cgtcgtcccg atgggtgctc
     1681 ggttcaaggt gggcccgttc gatctcgagt tcgtcacgat ggcccactcc ataccggagc
     1741 cctccgggct ggtgatccgt accccgcacg gcatcatctt ccataccgga gattggaagc
     1801 tcgaccgcac accgttggtc ggtgatccga cggatgagga gaaaattcgt agcctcggcc
     1861 gggaaggcgt ccgcgcgctg atctgcgact ccaccaatgc cttccgtgag ggccggtctc
     1921 cctcggaggc cgatgttgcc gagtcgctca aggcgatcat cgccaaggcg aagcgccggg
     1981 tggccgtgac gaccttcgcc tccaacgtcg cgcgcatacg tgccattgcc gaggcggcca
     2041 aggccacgcg acggcatctc gtcgtggctg gacgggccat gcatcgggtc atccaggtgg
     2101 ccatggagac aggctacctg ccgcaagatt tccgctatct cgaccaggat cagtttgcct
     2161 acctcgagcc cgacgaggtg ctgatgctgt gtactggcag ccagggcgag ccgcgggccg
     2221 cgctctcccg catcgcggaa ggtgagcatc cagacctccg cctcgggcaa ggcgacctcg
     2281 tcatcttctc gtcgcggacc atcccgggca acgagaaggc ggtcaccagg gtgcagaaca
     2341 acctggcgct gctcggggtg gatatcctca cggatgccga cgccctggtg catgtcacgg
     2401 gccacccgcg ccgcgaggag ctgaaggaga tgtacgcctg gctcaagccg gcggtcgccg
     2461 tgccaatgca tggcgaggcg cgccacctgc gggagcatgc ctggttggcc aaggcggcag
     2521 gcgttctgga tgtccatcct ttgcggaatg gacaaatgat ccgtctcgcg cctgatcccg
     2581 tggccattat cgacgaagcg ccggtggggc gcctttatcg agacgggcgg ctgatcgttt
     2641 ccggcgaggg ctctgtgcgg gagcgccggc ggcttgcctt tgccggaatc gtcgcgatcg
     2701 ggatggcgat gagccgccgc ggcgagctgc tgtccgatcc gagcatagcg atggagggca
     2761 ttccggccga gacggcggac ggcatgccca tgacagaggt ggttttcgac gccatcgatg
     2821 gcacgttgcg cagcatcccg gcaggcaagc ggcgggatat cgagctcgtg cgcgacgcgg
     2881 tgcggcgttc cgtgcgagcg gccatcgagc aggtctgggg caaaaagcct attgcgaagg
     2941 ttctcatcaa cgtcgtggat acgaggcgat agcaggcggt tggccgcatt gcgagagcag
     3001 ggtttggtgg ccagatcagg gggagggcac cgagagtcca gcgcggtcag cggcagccat
     3061 ctaacatcgg gcagtcggca ccgcggagtg gagcctcgca gcggctgcaa gggagcaaca
     3121 tcatgatcgg gcgcttgaac cacgtggcca tcgcggtccg cgaccttcaa gcagctgctg
     3181 cgacctatcg tgaaacgctt ggtgctacgg tctccgagcc cttgccgcag ccggaccacg
     3241 gcgtcactgt cgttttcgtg acgctgccga acaccaagat cgagcttctg gagccgctgg
     3301 ggccaaactc gcccatagcg aagtttctcg agcgcaatgc tgatggcggc atccaccacg
     3361 tctgctatga ggtggacgac atcattgccg cacgtgaccg cctaagagcc gcgggcgcga
     3421 gggtgcttgg cgatggcgag ccgaagattg gcgctcatgg caagcccgtt ctgttcctgc
     3481 acccgaaaga ttttttcggc acgctgatcg agctggagca ggcctgacca aagggcgcca
     3541 gatggagcaa caaaagtaat gagcctgaca ctggggatag cgctgtactt cattatctgg
     3601 tggacggtgc tgttcgccgt gcttccgatc
//
LOCUS       DASUMG010000154         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_661930, whole genome shotgun sequence.
ACCESSION   DASUMG010000154 DASUMG010000000
VERSION     DASUMG010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661930"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1790)
                     /locus_tag="VFX46_02495"
     CDS             complement(<1..1790)
                     /locus_tag="VFX46_02495"
                     /inference="COORDINATES: protein motif:HMM:NF005478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5599484.1"
                     /translation="MGAIARARQYIDSGAFEADLARRVAYRTESQLLPASLPQLESYL
                     RDEMEPILARMGFTCRIYPNPIEGQGPVLLATRIEDPGLPTVLGYGHGDVIRGLDDQW
                     TKGKGPWVTAREGDRLYGRGTADNKGQHTINLAALQAVLEERGRLGFNVKFMIEMGEE
                     AGSKGLNELVSAHREDFAADVLIASDGPRTRPERPTLTLGCRGALNFDLICDLREEME
                     PTLMRMGFTCRIYSNPIEGQGPVLLATRIEDPSLPTVLGYGHGDVIRGLDDQWTKGKG
                     PWVTAREGDRLYGRGTADNKGQHTINLAALQAVLQERGRLGFNAKFMIEMGEEAGSKG
                     LNELVSAHREDFAADVLIASDGPRTRPERPTLTLGCRGALNFDLVCDLREGGHHSGNW
                     GGLLANPAIILANAIATMVGPRGELLVEELKAPPMSKAVREALADVEIDGGEKGPQID
                     TWWGEPDLSPAERVYGANVLNVLAIGAGIPGKPVNAIPPRAVAHCQLRFVAGTDVANI
                     RPAVERHLRAKGFDRVQVADPPAANDGGFPATRTEPDDPWVIWAIDSFQKTANIKPAV
                     VPQMGGSICNDVFTDILGIPTLWVPHSYAS"
     gene            1934..>2618
                     /locus_tag="VFX46_02500"
     CDS             1934..>2618
                     /locus_tag="VFX46_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5599485.1"
                     /translation="MPLPPLRAHPEYQREIGYPQGVWRVSSFESRNIAGLITRLRASS
                     KAGKRNDPVAGAIWVTFAMATFAGLGTFGKYALQAGIHPLQVIFFRNFFCLVLMLPLL
                     HWRGRSLAMSAHFRLYGVRVTLTFVSMSAWFSALAYIPFGELTAIGFLAPLFATIFAV
                     LWLGEVVHVRRWTALAVGFVGALIILRPDTTGFGLGQILALVSAMTLGVVGPLLKQMS
                     AYDDPDKIVF"
BASE COUNT          430 a          854 c          821 g          513 t
ORIGIN      
        1 caggaggcat aggagtgcgg cacccacagc gtcggtatgc cgaggatgtc ggtgaaaaca
       61 tcattgcaga tggagccgcc catctgcggc acgacagccg gtttgatgtt ggcggttttc
      121 tgaaacgaat cgatggccca gatgacccag ggatcatcgg gctcggtgcg ggttgccgga
      181 aagccgccgt cgttggctgc ggggggatcg gcaacctgca cgcgatcaaa tccctttgcc
      241 cgaaggtgcc gctccaccgc cggtcggatg ttggctacgt ccgtgccggc aacaaaacgc
      301 agctggcaat gagccacagc cctaggcggg atggcattga ccggcttgcc agggatgcct
      361 gcgcctatgg cgagcacgtt caaaacattg gctccgtaga cccgctcggc aggcgaaagg
      421 tccggctccc cccaccaggt atcgatctgc ggaccctttt cgccgccatc gatctccacg
      481 tccgccagcg cctcgcgcac ggccttcgac atcggcgggg ccttcagctc ctcgaccagc
      541 agctctccgc gcggcccgac catcgtagcg atggcgttgg ccaggatgat cgcagggttg
      601 gccagcaggc cgccccagtt tcccgaatga tgcccaccct cgcgcaaatc acagacgaga
      661 tcgaagttga gcgcgccccg gcagccaagt gtcagcgtcg gccgttctgg acgcgtccgt
      721 ggtccgtcgg atgcaatgag cacgtcggct gcgaaatctt cgcgatgggc agagaccagc
      781 tcattcagcc ccttcgagcc cgcctcctcg cccatctcga tcatgaactt ggcattgaag
      841 ccgagccggc cacgttcctg aagcaccgcc tgaagtgccg ccaggttgat cgtatgctgg
      901 cccttgttgt ctgcggtgcc gcggccgtag agccgatcgc cctctcgcgc cgtgacccac
      961 ggccccttgc ctttggtcca ttggtcgtcc aacccgcgga tgacgtcgcc atggccgtag
     1021 ccaaggacgg ttggcaagct tgggtcctcg atgcgcgtag ccagaagaac tgggccttgg
     1081 ccttcgatgg ggttggaata gatgcggcac gtgaagccca tgcgcatcag ggtcggctcc
     1141 atttcctcgc gcagatcaca gatgagatcg aaattgagag cgccgcgaca gccgagcgtc
     1201 agcgttggcc gctcagggcg cgtccgtgga ccgtcggatg cgatcagcac gtcagccgcg
     1261 aagtcttcgc gatgggcaga gaccagctca ttgagcccct tcgagcccgc ctcctcgccc
     1321 atctcgatca tgaacttgac gttgaaccca agccgtccgc gttcctcgag caccgcctga
     1381 agtgccgcca ggttgatcgt atgctggccc ttgttgtcag cggtgccgcg gccgtagagc
     1441 cgatcgccct ctcgcgccgt gacccagggc cccttgccct tggtccactg gtcgtcgagc
     1501 ccgcggatga cgtcgccgtg gccgtagcca aggacggtcg gcaagccagg gtcctctatg
     1561 cgcgtggcca gcagcactgg gccttggccc tcgatcgggt tgggatagat gcggcacgtg
     1621 aagcccatcc gcgccagaat cggctccatc tcgtcgcgca gataggattc gagctgcggc
     1681 aggcttgccg gcaggagctg gctttccgtc cgataggcaa ctcggcgggc aagatcggcc
     1741 tcgaacgcgc cgctgtcgat gtactgccga gcgcgcgcga tggcgcccat tctggatgga
     1801 gctcgatccg ctgacattcc aagcccttcc cgaggcgatt aaccaaacga gactttgacc
     1861 cacagcgctt acggcgtcca gccggtccac cgccgccgca taactggcat gtcctgctca
     1921 ccgagcaatg gccttgcctc tgccacctct gcgagcgcat cctgagtatc agagggaaat
     1981 cggatatcct cagggcgtgt ggcgggtgtc gtcgttcgag agccggaaca ttgccgggct
     2041 gatcacccgg cttcgggcca gcagcaaggc gggcaaacgg aacgatcctg ttgccggggc
     2101 catctgggtc acgttcgcaa tggcgacgtt cgcggggctc ggcacattcg gcaaatatgc
     2161 gttgcaggcc ggcatccacc cgctgcaggt cattttcttc cggaactttt tctgcctcgt
     2221 gctgatgctg ccgctgctgc attggcgcgg gcggtccctc gccatgtccg cgcacttccg
     2281 gctttacggg gtccgcgtca ctctcacctt cgtgtcgatg tcagcctggt tctcggcgct
     2341 ggcgtacatc ccgtttggtg agctgacggc gatcgggttc ctcgcgccgt tgttcgcaac
     2401 gatcttcgcc gtgctgtggc tcggcgaggt ggtgcacgtt cggcgttgga ccgcgcttgc
     2461 cgtcggcttc gtcggtgcgc tcatcatctt gcggcccgat accacgggct tcgggctcgg
     2521 ccagatcttg gcgctggtct ccgcgatgac gttgggagtc gtcgggccac tgctcaaaca
     2581 gatgtcggca tatgatgacc ccgataagat cgtcttca
//
LOCUS       DASUMG010000155         3394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_742179, whole genome shotgun sequence.
ACCESSION   DASUMG010000155 DASUMG010000000
VERSION     DASUMG010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742179"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VFX46_02505"
     CDS             complement(<1..679)
                     /locus_tag="VFX46_02505"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX5599486.1"
                     /translation="MRSFHMPGRSPVYARRAMCATSHPLATQAAIEILREGGNAVDAA
                     LTAAAVLAVVEHPMTGIGGDCFAIIAKPGQKLIGLNASGRAPAAATAEWYASKGIKAI
                     EMQSPHAVTVPGAIDGWTTLLADHGSKPLSRILAPAIEYAEGGFPVAPRVAYDWANVA
                     EKLKGHPGSRQHLLPNGRPPRVGEVMRFPALARTLKIIAEQGRDGFYQGEVAEDIVAE
                     LRELGGLH"
     gene            complement(785..1291)
                     /locus_tag="VFX46_02510"
     CDS             complement(785..1291)
                     /locus_tag="VFX46_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HEX5599487.1"
                     /translation="MTFERRLAQLFRMDDEAWARHANPWSVWTRVISLPLLMLAVWSR
                     VWIGWWSLLPIAGVIFWLWINPRAFPPPRSTNNWASKGVLGERVWLNRREVPVPEHHR
                     LVPNVLTVLSAVGAIFFVWGLVRLEIWPTLLGLVLVMLSKLWFVDRMVWLYEDMKERH
                     PPYRAWLR"
     gene            complement(1322..3343)
                     /gene="accC"
                     /locus_tag="VFX46_02515"
     CDS             complement(1322..3343)
                     /gene="accC"
                     /locus_tag="VFX46_02515"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5599488.1"
                     /translation="MFKRILIANRGEIACRIIRTARRLGIETVAVYSEADRDALHVEL
                     ADTAVAIGPPPPSESYLVIDKIVAACKATGAEAVHPGYGFLSERAAFPRALAKAGVVF
                     IGPNPDAIDAMGDKIASKKAAAKAGVSTVPGHLGVIADEKQAVRIADQIGYPVMIKAS
                     AGGGGKGMRIAHNRRELEEEFDAARSEAKASFGDDRVFIEKFIVNPRHIEIQVLGDKH
                     GNVIHLGERECSIQRRNQKIIEEAPSPLLDEKTRKAMGEQAVALAKAVGYDSAGTVEF
                     VAGQDKSFYFLEMNTRLQVEHPVTEMITGIDLVEQMIRIAAGEELKIRQKDVTFTGWA
                     IESRIYAEDPFRNFLPSTGRLVKYRPPAQGSGEGMAVRIDTGVAEGGEISMYYDPMIA
                     KLITHAPTREAAIELQAQALDAFYIDGIKHNIPFLTALMQHPRWRAGNLSTTFIQEEY
                     PNGFEPRTPEGEDLQILAAVAAAIDHLHNCRRRKITGQMPGRPVTFAKRRVVDVGNGV
                     RRRVIVDGELNGPTTVTLLDDDGNPSETLEIASKWWPGEPVWSGMVRDRDVSVQVRPI
                     LNGFELSYRGVRVPARVYTEREAELAALMPVKAAADTSKVLLCPMPGLVKAIYVEAGQ
                     EVKAGDPLAVVEAMKMENTLRAERDGIVKLVNAKPGDSLAVDAVIMEFA"
BASE COUNT          613 a         1120 c         1045 g          616 t
ORIGIN      
        1 tgtggagacc accgagctcc ctgagctcag cgacgatatc ctcggcaacc tcgccctgat
       61 aaaagccatc acgcccctgc tcggcgatga tcttcagcgt gcgggcaagc gcggggaaac
      121 gcatcacctc gccgacacgc ggcggccggc cattcggcag caaatgctgg cgtgatcccg
      181 ggtgtccttt aagcttttcg gcgacgttgg cccaatcgta ggcgacgcgc ggcgcgaccg
      241 gaaatccgcc ctccgcatac tcgatggccg gagccagaat gcgggacagc ggcttggagc
      301 catgatcggc gagcagcgtc gtccaaccgt cgatggcacc gggtaccgtc accgcgtgcg
      361 gcgactgcat ctcgatcgcc ttgatgccct tggacgcata ccactcggct gttgccgccg
      421 ctggcgcgcg accggaggcg ttcaacccaa tcagcttctg tcccggtttt gcgatgatgg
      481 cgaaacagtc gccaccgatg cccgtcattg gatgctcaac aacggcgagc accgccgcag
      541 ccgtcagcgc cgcatccacc gcattgccgc cctcgcgcaa gatctcgatg gccgcctgag
      601 tcgcgagcgg atgtgacgtg gcacacatgg cgcgccgggc gtagacaggc gagcggccag
      661 gcatatggaa gctgcgcatg gaagatcccc tgattgagtg tcttgttggc gcaaatttgc
      721 gcatcgcgtt gcggtcagaa gccttggcca tgttgcccct gcacagctca ggcggcaagc
      781 cccgttacct gagccacgcg cggtaaggtg gatgacgctc cttcatgtcc tcgtacagcc
      841 agaccattcg gtccacgaac cacagtttgg aaagcatcac gaggacgagc cccaatagcg
      901 taggccagat ttcgagcctc accagccccc aaacgaagaa aatcgcgcct acggccgaga
      961 ggaccgtcag aacgttcggc accagccgat gatgctccgg caccggcacc tcacggcggt
     1021 tgagccaaac gcgttcgccc agcacgcctt tggaggccca attgttcgtg gaccgaggtg
     1081 gcgggaaggc gcgcggattg atccagagcc agaaaatcac acccgcaatc ggcaagagcg
     1141 accaccagcc gatccagacg cgactccaca cggccagcat caggagcggc agtgagataa
     1201 cgcgggtcca gacgctccag gggttggcat ggcgcgccca cgcctcgtca tccatccgga
     1261 agagctgggc gaggcgacgc tcgaatgtca ttggtcctcc tctcgagaac cagggcgcgg
     1321 gttacgcaaa ctccataatc acggcatcaa cggcgaggct gtcacccggc ttggcgttga
     1381 cgagtttcac gatgccgtcc cgctccgcgc gtagcgtgtt ctccatcttc atcgcctcga
     1441 ccacggcgag cggatcgcca gccttcacct cctggccagc ctcgacatag atcgccttca
     1501 caagaccggg catcggacac agaagcacct tggacgtatc ggctgccgcc ttgaccggca
     1561 tcagcgccgc aagctcggcc tcccgctccg tgtagacgcg cgctggcacg cgtacgccgc
     1621 ggtaagacag ctcgaaacca ttgagtattg ggcgaacctg cactgataca tcgcggtcgc
     1681 ggaccatgcc ggaccataca ggctcacccg gccaccattt cgatgcgatt tccagcgttt
     1741 cggacgggtt tccgtcatca tccagcaatg tcaccgtggt cggcccattg agctcgccgt
     1801 ccacgatcac ccgccgacga acaccgtttc cgacatcgac gacgcggcgc tttgcaaaag
     1861 taacaggccg gccaggcatc tgacctgtga tcttccgtcg tcggcaattg tggaggtgat
     1921 cgatcgcagc agcgacagcc gcgagaatct gcagatcctc gccttcggga gttcgaggct
     1981 cgaagccgtt gggatactcc tcctgaatga acgtggtcga aagattgccc gcccgccaac
     2041 gcgggtgctg cattaaagcg gtgaggaacg gaatattgtg cttgatgccg tcgatgtaga
     2101 aggcgtcgag cgcctgcgcc tgtagctcga tggccgcctc gcgcgtgggt gcgtgcgtga
     2161 tgagcttggc gatcatcggg tcgtagtaca tcgagatctc gccaccctcg gccacgccgg
     2221 tgtcgatgcg cacggccatg ccttcccctg acccctgagc gggagggcga tacttcacga
     2281 ggcggccggt ggacggcagg aagttgcgga agggatcctc ggcgtagatg cggctttcga
     2341 tcgcccagcc ggtgaaggtc acgtccttct gccggatttt gagctcctcg ccggcagcga
     2401 tccggatcat ctgctcaacg aggtcgatcc cggtgatcat ttctgtcacc ggatgctcga
     2461 cctgcaggcg ggtgttcatc tcgaggaaat agaagctctt gtcctgcccg gcgacaaact
     2521 ccaccgtgcc ggccgagtcg tagcccacgg cctttgcaag cgcgacggcc tgctcgccca
     2581 tggccttgcg ggtcttctcg tcgagcagcg gcgatggcgc ctcctcgatg attttctggt
     2641 tgcggcgctg gatcgagcat tcgcgctcgc cgagatgaat gacattgccg tgcttgtccc
     2701 ccagcacctg aatttcgatg tgccggggat tgacgatgaa cttctcgatg aaaacccggt
     2761 cgtcgccgaa gctcgccttc gcctcggagc gcgccgcgtc gaactcctcc tccagctctc
     2821 tccggttgtg ggcgatgcgc atgcccttgc cgccaccgcc ggcggacgcc ttgatcatca
     2881 cgggatagcc gatctggtcg gcgatcctca ccgcctgctt ctcgtcggcg atcacgccga
     2941 gatggccggg aacggtggat accccggcct tcgccgccgc cttcttgctc gcgatcttgt
     3001 cgcccatggc gtcgatggcg tcgggattgg gaccgatgaa gacgacgccg gccttggcca
     3061 gcgcccgcgg aaacgcggcc cgctcggaga ggaatccgta gcccggatgc acggcctcgg
     3121 cgccggtcgc cttgcaggcc gcgacgatct tgtcgatgac gaggtagctt tccgacggcg
     3181 gcggcgggcc gatcgcaacc gcggtgtcgg ccaattccac gtgcagcgcg tcgcggtcgg
     3241 cctcggagta gaccgcgacc gtctcgatgc cgaggcgccg cgcggtcctg atgatcctgc
     3301 aggcgatctc gccgcgattg gcgatcagga tgcgcttgaa catgcccgac taaaccccct
     3361 tccggctctc aggctttgcg gcgttgcagc agga
//
LOCUS       DASUMG010000156         2865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_501415, whole genome shotgun sequence.
ACCESSION   DASUMG010000156 DASUMG010000000
VERSION     DASUMG010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501415"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VFX46_02520"
     CDS             <1..780
                     /locus_tag="VFX46_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX5599489.1"
                     /translation="TRLDPEFVRPIAHRGLHDISLGRIENTAPAFEAAIAAGFGIECD
                     LRPASDGTPFVFHDRTLDRLVEATGPIASYSPSKLSVLRYRNQNTRLLSFGDLLELVA
                     GKVPLLVEIKSDWDTPDSQFLAAVADLSLGYRGPLALMSFDPAVMAAMRNLAPGLPRG
                     IVSGSYEGPEWWSEAIDSERAFRLRHCLEAGPAQPSFIAYHVDDLPTPVTRFAREVIR
                     LPVFAWTVRTEAQLRHAAAWADAPIFEGCDPHSCVTGACSS"
     gene            884..1204
                     /gene="sugE"
                     /locus_tag="VFX46_02525"
     CDS             884..1204
                     /gene="sugE"
                     /locus_tag="VFX46_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HEX5599490.1"
                     /translation="MAWVVLFVAALLEVAWAIGLKYTQGFTRLWPSVITLAAMVASMY
                     LLALAARTLPIGTAYAVWVGIGATGTAILGILLFDESADPARLVCLGLIIAGVLGLRL
                     LDST"
     gene            1287..2477
                     /locus_tag="VFX46_02530"
     CDS             1287..2477
                     /locus_tag="VFX46_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5599491.1"
                     /translation="MTSTGTAIVRIATSMAEIDAAQWDACANPDPARFNPFVTHAFLN
                     ALEQAGTVNRRTGWLPQHLVLEGPDGKIAGAMPCYLKGHSRGEYVFDWAWADAYERAG
                     GTYYPKLLCAVPFTPVPGPRLLVRPGPEQHNVERTLAAAAITLAKKLGVSSLHINFLT
                     EGEWQRLPELGFLQRTDQQFHWHNQGYSSFDDFLASLASRKRKAVRRERAQALAPGIT
                     IEHVTGADIREEHWDAFFDFYMDTGSRKWGRPYLNRRFFSLLGEVMADRCLLVMAKRQ
                     GRYIAGALNLIGGDCLYGRYWGAIEHHPCLHFEVCYYQAIEYAIANKLPRVEAGAQGE
                     HKLARGYMPVPTYSLHWIADPNFRRAVARYLEEERREVAEHRDILATYGPYRKVAPEA
                     EEAC"
     gene            2513..>2865
                     /locus_tag="VFX46_02535"
     CDS             2513..>2865
                     /locus_tag="VFX46_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5599492.1"
                     /translation="MTYDNNNVFAKILRGELPAHKVYEDEHTLAFMDIMPRTKGHTLV
                     IPKTPARNILDATPEQLAACMATVQRVARAVMKAFDAPGVTIQQFNESAGGQVVFHLH
                     FHVLPRWEGVPLAPHT"
BASE COUNT          529 a          898 c          856 g          582 t
ORIGIN      
        1 acgcgtcttg atccagagtt cgtgcgcccc atagcgcatc gcggcctgca cgacatctcc
       61 ttgggacgga ttgagaatac cgcgccggcc tttgaggctg ccattgcggc gggctttggt
      121 atcgaatgcg atctccgccc cgcgagcgac ggaacaccgt tcgtgttcca cgatcggacg
      181 ctcgatcgac ttgtagaagc tacgggtccg atcgcgtcgt acagcccatc caagctctct
      241 gtattgcgct accgcaacca gaacacacgg ctgctcagct tcggagacct cctcgagctg
      301 gtcgccggca aggtaccgct gctcgtcgag atcaagagcg actgggatac gccggattcg
      361 caatttctcg cggcggtggc agatctctcg ctcggctaca gggggccact cgccctgatg
      421 tcgttcgatc ccgcagtcat ggccgcgatg cgtaatcttg cgccagggct gccgcgcggc
      481 atcgtctccg gttcctacga ggggccggag tggtggtcgg aggctattga ttccgaacga
      541 gcctttcgcc tccgccactg tctggaggcc ggaccggcac aaccaagctt catcgcttac
      601 catgttgacg atctgcccac gcctgtgacg cgcttcgccc gtgaagtcat acgattacct
      661 gtctttgcct ggacggtgcg cacggaggcg caactccgcc atgctgcagc gtgggcggat
      721 gcccccatct tcgaaggatg cgatcctcat tcctgcgtca ccggcgcgtg ctcgtcttga
      781 ccccttgaac tgaccggcta agtcaatgtg ccactggccc gtggggacga cttcacagca
      841 agagggccaa tgcagggacg gccctgcgat ctgagaggat ttcatggctt gggtggtgct
      901 attcgtcgcc gctttgctgg aagtcgcgtg ggctattggt ttgaagtaca cgcaagggtt
      961 cacacgatta tggccgtctg tcatcacatt ggcggccatg gtagcaagca tgtatctgct
     1021 ggcgctcgca gctcgaacac tccccattgg gacggcctat gccgtatggg tcgggatcgg
     1081 cgcgactgga acggctatct tgggaattct gcttttcgat gaatcggccg atcccgccag
     1141 acttgtctgc ctcgggctga tcatcgcggg cgtgctcggt ctacggctac tcgacagcac
     1201 ttgaactgag ccccaagtca gtagctgcgc atcgaactcg tagcacaacc tgatatgcta
     1261 tcggctagag ccgatccgga gtgacgatga catctactgg caccgcaatt gttcgcatcg
     1321 ctacctccat ggccgaaatc gacgcggccc agtgggatgc gtgcgccaat ccggatcccg
     1381 cgagattcaa tccgttcgtt acccatgcct tcctgaatgc actggagcag gccggcaccg
     1441 tgaaccggag gacgggctgg ctaccccagc accttgtttt ggaggggccg gacggaaaaa
     1501 ttgccggcgc tatgccgtgc tatctgaagg gccacagccg gggcgagtat gttttcgact
     1561 gggcttgggc agacgcctac gagcgcgccg gcggcaccta ttatccgaag ctcttatgcg
     1621 cggtcccgtt caccccggtt ccagggccac gactgctggt tcggcctggc cccgagcaac
     1681 ataacgttga acgtacgctt gccgctgccg cgatcacgtt ggccaagaag ctcggcgtgt
     1741 cctcacttca catcaacttt ctcactgagg gtgaatggca gcgcctgccg gagctaggct
     1801 tcctgcagcg cacggatcag cagttccatt ggcataacca aggctacagc tcgttcgacg
     1861 atttcctcgc gagcctcgcc tcgcggaaac gcaaggccgt gcgcagagag cgcgcgcagg
     1921 ccctcgcccc cggcatcaca atcgagcatg tgacgggagc cgacatccgc gaagagcatt
     1981 gggacgcttt cttcgacttc tacatggata ccggctcccg caagtggggc cggccctacc
     2041 tcaaccggcg gttcttttca ctgctcggcg aggtcatggc ggacagatgt ctgctcgtca
     2101 tggctaagcg gcaaggccgc tacatcgccg gagctctgaa cctgatcggc ggtgattgcc
     2161 tttatggccg ctactggggc gccatcgagc accatccgtg tcttcacttc gaggtctgct
     2221 actaccaggc tatcgagtac gcgatcgcca acaagctgcc gcgcgtcgag gccggggctc
     2281 agggcgaaca caagctcgcc cgcgggtata tgccggtgcc gacgtattcc ctacactgga
     2341 tcgccgatcc gaacttccgc cgcgccgttg cccgctatct tgaggaggag cgccgggagg
     2401 tggcagagca cagagatatt ctggcgactt acggacccta ccggaaagtc gcgcccgagg
     2461 ccgaggaagc gtgttgaggt ttactggcgt ccacagctga aaggactggt tgatgaccta
     2521 cgacaacaac aacgttttcg ccaagattct ccggggtgag ctgccagccc acaaggtcta
     2581 cgaagacgag catacgcttg ccttcatgga catcatgccg cggaccaagg gccatacgct
     2641 tgtgatcccg aagacgccgg cacgcaacat cctcgacgcc acgccggagc agctcgccgc
     2701 ttgcatggcc actgtacagc gcgtcgctcg cgccgtgatg aaggccttcg atgcgccggg
     2761 ggtgacaatc caacagttca atgaatcagc tggcggacag gtcgtttttc acctccattt
     2821 tcacgtgctg ccgcgctggg agggcgttcc gcttgcgccg catac
//
LOCUS       DASUMG010000157         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_180540, whole genome shotgun sequence.
ACCESSION   DASUMG010000157 DASUMG010000000
VERSION     DASUMG010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180540"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2597)
                     /locus_tag="VFX46_02540"
     CDS             complement(<1..>2597)
                     /locus_tag="VFX46_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX5599493.1"
                     /translation="AMTGIAEWLASFGLSEYAERFRENAIDLSVVRDLTEQDLKDLGV
                     LLGHRRKMLRAIADLRGDLPRTPRTGTRPASADNAGRRQLTVLFCDLVGSSALSARLD
                     LEDLRTVMGAYHRCVAGIVAQNEGVIARYMGDGVLAYFGYPQAHEDDAEQATRAGLAL
                     VDAVARLRTDPATELQVRVGIATGMVVVGDLTGEGSAQEQTVIGETPNLAARLQTFAN
                     PGTVLISESTHHLTDGHFECRNLGPVALKGWAEPIPAWQVLGATEVESRFEAQHKTRL
                     APPIGRDEEIEMLLRRWHRARFGEGCAVMLTGEPGIGKSHIALALDERLESEPHITIR
                     QFCSAHHTNSALYPFIRQLERAARFERSDLPAEKFAKLEALLARSRADADRVVVPLAN
                     LLSIPPSDGYRLPELSPQKRKEVTLAAFLAQFSGLAARAPLFAIVEDVHWADPTSLEL
                     LTMALEQLSRLRVLMLVTARPEFTPPWPGHAHVTTISLTRLNRRNGAALIERVTSGKT
                     LPEEVMDQILARTDGVPLFVEELTKTVLETGLLHEQDNHYVLNRPLPSMAIPTTLHAS
                     LMARLDRLAPVREVAQIGAVVGREFSYELLSTVAELPKERLEDALDQLVRSELIFRRG
                     ENPQAVYTFKHALVRDAAYSGLLKSRRAALHATIADTFEQRFPEIAETQPETLAHHLT
                     EAGLFEKAVAYWLQAGKKAAMRSANLEAIAHLQRGIEASGHLSDSARKDRLELDFQFA
                     LGPCLIATQGPASNKTMATFSRARALCERLGDPPEQHQVMFWLTTASVMRGELPLAEE
                     TIAVLIQLAEARDDRPALLNAMRGQGMIRLFMGHLTGAQEVIGRAYDAFEASSEDDRL
                     AARSAGQDA"
BASE COUNT          461 a          865 c          806 g          465 t
ORIGIN      
        1 cggcatcctg gccagccgac cgcgccgcca gcctgtcgtc ctcgctgctc gcttcgaatg
       61 catcgtaggc acgtccgatc acttcctgag caccggtgag gtgtcccatg aaaaggcgga
      121 tcattccctg cccgcgcatc gcattgagca acgccggtcg atcatcgcgc gcttcggcga
      181 gctggatcag aaccgcgatg gtttcctcgg ccagcggcaa ctcaccacgc atgacgctgg
      241 cggtggtcaa ccagaacatc acctggtgtt gctcgggagg gtctccgagg cgctcgcaca
      301 gcgcacgcgc gcgcgaaaac gttgccatcg tcttgttcga ggccggccct tgggtagcga
      361 tcaggcaggg cccgagagcg aactggaagt ccagttccaa cctgtccttt cgcgcgctgt
      421 cggatagatg gcccgacgcc tcgatacctc gttgcaagtg cgcgatggct tcaaggttgg
      481 ccgaacgcat ggcggccttc ttgccggcct gcagccaata ggccaccgcc ttttcgaaca
      541 gcccggcctc cgtcagatga tgcgcgagag tttcgggttg cgtctccgcg atttccggaa
      601 accgttgctc gaaggtatcc gcgatagtgg cgtgcagcgc tgcgcgccgg cttttcagca
      661 gtcccgaata ggcagcgtcc cgcaccagcg catgtttgaa ggtatagacc gcctgggggt
      721 tctcgccccg gcggaatatc aactctgacc gaaccaactg gtcaagcgca tcctcaagcc
      781 gctcttttgg caattcggcc acggtgctca gcagctcgta ggagaactcg cggcccacca
      841 cggcgccgat ctgggccacc tccctcaccg gagccaaccg atcgagtctc gccatcagcg
      901 atgcgtgcaa cgtcgtcgga attgccatcg agggcagcgg acggttgagg acgtagtgat
      961 tgtcctgctc gtgcaacagc ccggtttcga gcaccgtctt ggtcagttct tccacgaaca
     1021 ggggcacgcc atcggtgcgg gcgagaatct ggtccatcac ctcttccggg agcgtcttgc
     1081 cggaagtgac gcgctcgatc aacgctgccc cgttgcgccg gttcagccgt gtcagcgaga
     1141 tcgtcgtcac atgcgcatga cccggccagg gcggcgtgaa ttccggcctc gccgtgacga
     1201 gcatcagcac ccgaagccgc gatagctgct ccagggccat cgtgagcagc tccagcgaag
     1261 tgggatccgc ccaatggaca tcctcgacaa tggcgaacaa tggcgctcgc gccgccaggc
     1321 cactgaattg agccaggaac gccgccaatg tcacctcctt gcgcttctgc gggctgagct
     1381 ccggcaggcg gtagccgtcg ctgggcggta tcgagagcag gttggccaga ggcaccacga
     1441 cgcggtcggc gtcggcgcgg gaccgcgcaa gcagcgcctc gagcttggcg aacttctctg
     1501 ccggcaggtc gctccgctcg aaccgcgcag cccgttcgag ctggcggatg aacggataca
     1561 gcgcactgtt ggtgtgatgc gccgagcaga actggcgtat cgtgatgtgc ggttcgctct
     1621 cgagccgctc gtcaagcgcc agggcaatgt gcgacttgcc gataccaggc tcaccggtga
     1681 gcatcaccgc gcaaccttcg ccgaacctgg cgcgatgcca gcgacgcaac agcatctcga
     1741 tttcctcgtc gcgcccgatg ggcggcgcca atcgggtttt gtgctgcgcc tcgaaacggc
     1801 tttccacttc ggtcgcgcca aggacctgcc aggccggtat tggctccgcc caacctttaa
     1861 gcgcgacagg gccgagattg cggcattcga aatgcccgtc ggtgaggtgg tgagtactct
     1921 cggaaatcag caccgtcccg ggattggcga acgtctgcag gcgcgcagcc aggttcggcg
     1981 tctcgccgat gaccgtctgc tcttgcgccg agccttcgcc ggtcaaatct cccaccacca
     2041 ccatgccggt cgcgatgcca acgcgaacct gcagctcggt ggcggggtct gtccggagcc
     2101 tggccacggc atcgaccagc gccagcccgg cgcgtgtcgc ctgttcggca tcatcctcgt
     2161 gcgcctgggg gtagccgaaa taggcgagca cgccgtctcc catataccgc gcgataacgc
     2221 cctcattctg ggcaacaatc ccagcgacgc agcggtgata ggcgcccatc acggtccgca
     2281 agtcctccag atcaaggcgg gccgaaagtg ccgacgagcc gaccagatcg caaaacaaga
     2341 ctgtaagttg acgccgcccg gcgttgtccg ccgacgccgg cctggtgccg gttcgaggtg
     2401 ttcgcggcag atctccccga aggtcggcga tcgcgcgcag catcttgcgg cgatgcccaa
     2461 gcaggacgcc caggtccttg agatcctgct ccgtgagatc tcgaacgacc gagaggtcga
     2521 tggcgttctc acggaatcgc tcggcatact cgctcaggcc gaacgatgct agccactctg
     2581 cgatacccgt catggca
//
LOCUS       DASUMG010000158         3770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_581872, whole genome shotgun sequence.
ACCESSION   DASUMG010000158 DASUMG010000000
VERSION     DASUMG010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581872"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFX46_02545"
     CDS             <1..550
                     /locus_tag="VFX46_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5599494.1"
                     /translation="FGVLDGRFVERMPIRPDEHFEPAGCSGLIVEAFTVPGKVALYLE
                     DEQAGSDLGTAPGDTIGLKLTDTRSGRFACYVPACAAVDDTLLRRMSGAATILFDGTL
                     FTDDEMIAQGLSPKTGRRMGHISMSGPDGSIAALGRLDVGRRIFTHINNSNPVLRQGS
                     PERARVEAAGWEIAYDGMEVIL"
     gene            559..1284
                     /gene="pqqC"
                     /locus_tag="VFX46_02550"
     CDS             559..1284
                     /gene="pqqC"
                     /locus_tag="VFX46_02550"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX5599495.1"
                     /translation="MTPDELEQQIRAVGAERYHDKHPFHRLLHSGKLNKGQVQAWALN
                     RYCYQAAVPRKDAALMSRTTDRELRREWIHRILDHDGSGDEEGGIERWLVLTDGLGLD
                     REYVTSMAGALPATRFAVEAYVAFVRERSLVEAVASSLTELFAPSIHRERIAGMLKNY
                     DFIDDSVMAYFRRRLNQATRDADFALDYVKRHATTRELQELCIEAVRFKCNVLWAQLD
                     ALHHAYVTPGLIPPGAFKPQPSM"
     gene            2179..3282
                     /locus_tag="VFX46_02555"
     CDS             2179..3282
                     /locus_tag="VFX46_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599496.1"
                     /translation="MARELRDAPPPLSLLDFPPEDDGIAGAKLAIADNNTTGRFLKQE
                     FTLDVIEAAADELVLRVKEKASAGAGFVVADAAPNTVLALSAALDGQNALVFNAGSGD
                     DALREENCRKNVVHTAPSRAMLADALAQYLVWKRWRRWFLVHGTQPDDLAYAAALRRA
                     ANRFGAKIVEERAYEYEVGSRRADGGHEQIQQQIPTFTQNVPDYDVLVIADESDQFGE
                     YIPYMTWDARPVAGTQGLVAASWHPALEQWGGTQFQSRFQKLANRPARPLDYNVWIAI
                     RSIGEAATRTRSADPEKLIAYLKSAEFDVAGFKGRKLTFRAWNGQLRQPILLSTPKLP
                     LTVSPQPGFLHQFSELDTLGVDEPESKCSNYAP"
     gene            3353..>3770
                     /locus_tag="VFX46_02560"
     CDS             3353..>3770
                     /locus_tag="VFX46_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX5599497.1"
                     /translation="MSLAAGSILFAPQISAQTVFVTNEKDNTVTVIDGDTLEAVKTVK
                     VGQRPRGVTLSKDEKWLLICASDDNTVQVYDARTMEFVKSLPSGPDPELFILHPSGNP
                     LYIANEDDNLVTVVDIEKGTVLAEIPVGVEPEGMGIS"
BASE COUNT          748 a         1165 c         1122 g          735 t
ORIGIN      
        1 tttcggcgtg ctggatggac ggttcgtgga gcggatgccg attcggccgg acgagcactt
       61 cgagccggcg ggctgttcgg gacttatcgt cgaggcattc acggtgcccg gcaaagttgc
      121 cctttatctc gaagatgaac aggccggatc agatctcggc accgcgcccg gcgacaccat
      181 cggcctgaaa cttaccgaca cccgttcagg tcgcttcgcc tgctacgtgc ctgcctgcgc
      241 cgccgtcgac gacacattgc ttcgacggat gtcgggtgca gcaaccatcc tgtttgacgg
      301 cacgctcttc actgacgacg agatgatcgc gcaggggctg tcgccgaaga ccggccgtcg
      361 tatgggccat atcagcatgt ctggccccga cggttccatc gcggccctcg gcagactcga
      421 cgttggacgc cgcatcttca cgcacatcaa caactcgaat ccggtgctgc ggcaaggctc
      481 gcccgaacgt gcccgggtcg aggcggccgg atgggagatc gcctacgacg gcatggaggt
      541 catcctgtga gaccaccgat gacgccagac gagctcgagc agcagatccg cgcggtcggc
      601 gccgagcgct atcacgacaa gcacccgttt caccgtctcc tccactccgg caagctcaac
      661 aagggccaag tgcaggcctg ggcgctcaat cgctactgct atcaggccgc agtgccacgg
      721 aaggatgccg ctctgatgag ccgcaccaca gaccgggagc tgcggcgcga atggatccac
      781 cgcattctgg accacgacgg cagcggggac gaggaaggcg ggatcgagcg ctggttggtg
      841 ctgacggacg gcctcggact cgatcgggag tacgtgacct ccatggccgg tgcattgccc
      901 gccacccgat tcgctgtcga ggcctatgtc gcattcgtcc gggagcgatc gctggttgag
      961 gcggtggcct cctcgctgac cgagctgttc gcgccatcga tccaccgcga gcgcattgcc
     1021 ggcatgctca agaactacga cttcatcgac gactccgtga tggcttactt ccgccggcgc
     1081 ctaaaccagg ccacccgcga cgcggatttc gcgctcgact acgtcaaacg gcacgccacc
     1141 acgcgcgagt tgcaggagct ttgcatcgag gcggtccggt tcaagtgcaa tgtgttgtgg
     1201 gcgcagctgg acgcactgca tcatgcttac gtcacgcctg gcctcatccc acccggtgct
     1261 ttcaagccac agccaagtat gtgaagcagc agcgcttgcg aacaccctag cgatgggcta
     1321 gaccgtggcg cgattgcgag ttgaatgcga gggctggcct cgctatgtgt gttggtcagc
     1381 tttcgaggag acacgctgcc gtagctcagt ggtagagcac tcccttggta agcttgcccg
     1441 cctgaccgga aacggtcagg agcgtaccgc ccaaagtcgg ggaaaccgtc cggtccggcc
     1501 gacggcaatc ccgagccaag cccgggacac gggaaggtgt agaggccaga cgggtggcat
     1561 ctaagggtcc ctcgggacct aagatgaagg gatggtccag cgcacgaacg cccacagtgg
     1621 cgggcgcgaa agcgcaggtg tgatgaaggg agaggtcgtg cgttcaatcc gcaccggcag
     1681 caccatcgtt cccaggccgc tcattcgggc ggtctttttt gtttcatttg gcgctgttgg
     1741 aattgttcct aagggaggga tgttccattc ccaaccttaa gaaacctgtt cggatgaaat
     1801 cggttccctc ctttctgatt atcttcagta ttggttagac gcccgtttta cgcttattcg
     1861 ggttttatcg actcgccctc cgctcttttc ttgacaatac cgaacacaca cgcgagcatt
     1921 ttctaatcgg atggctttgg cggaaaaact cccgcaattc ttcttgcggc cgccaactgg
     1981 aggccacgta tgcagagttc cgtttggttg cgctctcttg ttcgccgccg cctctgcagg
     2041 ccatggcttc cgctgctcgc tattacggct tttgcagcgc ccctcacttt acctgtagat
     2101 gctcgcggcc agagcactga tccaacaacc gccaagcaaa caagccgtca aaacgaggcg
     2161 caaaggatta ccatcatctt ggcgcgcgag ctgcgcgatg ctccgccgcc gctgtctctg
     2221 ctcgatttcc ctcccgagga cgacggcatt gccggtgcca agctcgccat agccgacaac
     2281 aatacgacgg gccgcttctt gaagcaggag ttcacgctcg atgtcataga agctgccgca
     2341 gacgagctag tcctgcgtgt caaggagaag gcatcggccg gggctggatt cgtcgttgct
     2401 gatgccgctc cgaatacggt tctcgctttg tcggcagcgc tcgatggaca gaatgccctc
     2461 gtcttcaatg caggcagcgg cgatgatgcc cttcgagagg agaattgccg gaaaaacgtg
     2521 gtccacaccg cgccgtcacg cgccatgctg gccgatgcct tggcccaata tctcgtttgg
     2581 aagcgctggc ggcgctggtt tctagtccac ggcacccagc cggatgatct cgcttatgca
     2641 gcggcattgc gtcgggccgc caaccgtttc ggggcgaaaa tcgtcgagga gcgggcatac
     2701 gagtatgagg tcggcagccg ccgtgccgac ggcggccatg agcaaatcca gcaacaaatt
     2761 ccgaccttca cccaaaatgt accggattac gacgtgctgg tgatcgccga cgaaagcgac
     2821 cagtttggcg agtacattcc atacatgacc tgggatgcgc gcccggtagc cggaacacaa
     2881 ggattggtgg cagcgagctg gcatcccgcc ctagagcaat ggggcgggac acaattccag
     2941 tctcgctttc aaaagctcgc taaccggcca gcccgtccgc tcgattacaa cgtctggatc
     3001 gcgatacgat cgatcggcga agcagcgaca cgcaccagat cggcggatcc cgaaaaattg
     3061 atcgcttacc tgaaatccgc tgagttcgac gttgccgggt tcaagggacg caagctcacc
     3121 ttcagggcct ggaatggcca gctgcgccag cctattctcc tttctacgcc gaagctgccg
     3181 ctgacggtgt cgccgcagcc cggtttcctg caccagttct cggagcttga taccctcggg
     3241 gttgatgagc ccgagtcgaa gtgtagcaac tatgcgccgt gatcaacgcg gccagggggc
     3301 gggatagcgt catgaggagg cttccgtgtc ccgtattgga accgccctgt ccatctcact
     3361 cgccgcgggc agcattctct tcgcgccgca aatttctgcc cagacggtat tcgtcacgaa
     3421 cgagaaggat aacactgtaa ccgtcatcga cggcgacacg ctggaggccg tgaaaactgt
     3481 caaggtcggc cagcgacccc gcggggtcac gttgtccaaa gacgagaaat ggctgctgat
     3541 ctgcgccagc gacgacaata ccgtccaggt ctatgacgcc cgcacaatgg aatttgtgaa
     3601 gagcctgccg tccggtcccg atcccgagct cttcattctc cacccatccg gaaatccgct
     3661 ctacatcgcg aacgaagacg acaacctcgt caccgtggtt gacatcgaga agggtaccgt
     3721 gttggccgaa attccagtcg gcgttgaacc ggaaggaatg ggcatcagcc
//
LOCUS       DASUMG010000159         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_100828, whole genome shotgun sequence.
ACCESSION   DASUMG010000159 DASUMG010000000
VERSION     DASUMG010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100828"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VFX46_02565"
     CDS             complement(<1..325)
                     /locus_tag="VFX46_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599498.1"
                     /translation="MTATIGLFRALTIPALRLCRTFAIFTLRLLRALSVSAGRLFGFL
                     TSPIRLLRSFQLPSFGLFRLLPFRAFTLLLDLTPTTLSRRRLFAPPGLIRGHLPSRGA
                     LPLAGG"
     gene            350..898
                     /locus_tag="VFX46_02570"
     CDS             350..898
                     /locus_tag="VFX46_02570"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HEX5599499.1"
                     /translation="MSERQRTAVVQRFRESGAIDRARVTGVDIDISVGARVPRERIRL
                     APVPTVIVEEVPAYRGHLFFVVRDEIVIVEPSTYVVVDVIRLDGAPGRAAQADGGRLT
                     LTSEQREIVLSHIDMQPEVRLGIGDVSVGMSIPRTVQLRSFPQAVIEDVPELEGYRYF
                     VFEDEVAIVDPDERRVVLTISG"
     gene            complement(943..1326)
                     /locus_tag="VFX46_02575"
     CDS             complement(943..1326)
                     /locus_tag="VFX46_02575"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5599500.1"
                     /translation="MRRINPDDIAKPASNYVQGVVHTLGGERLVISGQTGDRPDGSIE
                     EGLEAQMRRAWSNLFGVLRAAGFEKRHLVKVTIFVTVPGAVPTARKIREEALEDHLCA
                     STYLQVAGLARPEYLVEIEAEAVKE"
     gene            1588..2331
                     /locus_tag="VFX46_02580"
     CDS             1588..2331
                     /locus_tag="VFX46_02580"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX5599501.1"
                     /translation="MSDTEQTEAGRPPDAAWVNVGPQERTASALLGSLLLAWGARRGR
                     LIGTIAALGGAALLARGATGHCPGYATLRPTEEETRFARERGWSTAVVARESIVIDRP
                     PEELYRFWRDEINLPRFMESLEHVEKLSDRSSRWTVLGPAGRRLHWVSTITEDRPSEF
                     IAWRAEENADIRNAGWVEFRDLGSGTSTRVETFMAYEPPIGLAGHAIASLLGRDPASA
                     MRRSLQRLKQVVESEMEQENKQPTGAEQY"
     gene            2550..3293
                     /gene="otsB"
                     /locus_tag="VFX46_02585"
     CDS             2550..3293
                     /gene="otsB"
                     /locus_tag="VFX46_02585"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5599502.1"
                     /translation="MTLPDLDFEKDALFLDLDGTLIDIAPHPDDVVAPPDLIRSLGAL
                     ERRLGGALSVISGRPIAAIDALLAPLRLPASGVHGAEIRFEGGQANIARAAPPIPNGV
                     RWLLAPLAEVPGVLIEDKTIAIAVHFRQAPAAAERVREVVEAAVSAHRADQLTIVPGK
                     CVLEIKHARFSKGTAVEEFMARPPWAGRRPVFIGDDVTDEAAFAVLPKWKGVGIAVGQ
                     PRPGAVAGFATASEVRNWLAGLAREECTT"
     gene            3290..>3458
                     /locus_tag="VFX46_02590"
     CDS             3290..>3458
                     /locus_tag="VFX46_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX5599503.1"
                     /translation="MSAPGLDYGLIGNGRIAALIDKNGRIVWWCFPRLDGNPVFSRLL
                     AGDEDKGYTDVV"
BASE COUNT          646 a         1084 c         1094 g          634 t
ORIGIN      
        1 ggcccccggc cagcggcagg gcgcccctgg agggcagatg acccctgatc agccccggcg
       61 gggcgaacag cctccggcga ctgagcgtcg tcggggtcag gtccagcaac agcgtgaacg
      121 cacgaaacgg gagcaggcgg aacaacccga aggaaggcag ctgaaaggag cggagcaacc
      181 ggatcggcga cgtcagaaag ccgaagagcc gacccgccga gacagacagc gcgcggagga
      241 gcctgagcgt aaagatcgcg aacgtgcggc acaaccggag cgcagggatc gtgagcgcgc
      301 ggaacagccc gatcgtcgcc gtcaagcgga gcgtccggat cggccgcggg tgagtgagcg
      361 gcagcgcacc gcagtggttc agcgtttccg tgaaagcggt gcgatagatc gcgcccgggt
      421 caccggtgtc gatatcgaca tctccgtcgg cgcccgagtt cctcgcgagc gcatccgtct
      481 ggcgcctgtg ccgacggtga tcgtggaaga ggtccccgca tatcgcggcc acctcttctt
      541 tgtcgtgaga gatgagatcg tcattgttga gccctcgacc tacgtcgtcg tcgacgtaat
      601 ccggcttgat ggagcaccgg gccgggcagc gcaggcggat ggcggccgac tgacactcac
      661 cagcgagcag cgtgaaatcg tgctatctca catcgacatg cagccggagg tccgcctcgg
      721 cattggcgat gtcagcgttg gcatgagtat tccgcgaact gtgcagctgc gctcgttccc
      781 gcaagcggtg atcgaggatg ttccggagct cgaaggctat cgttactttg ttttcgagga
      841 cgaagtggcg atcgttgatc ctgacgaacg tcgggtggtt ctcaccatca gcggctaagc
      901 gttatgccgg tgcccgtctc gcgaagacgg gcaccggttt tctcactcct tcaccgcctc
      961 ggcttcgatc tcgacgagat attccggcct cgcaagcccc gcgacctgca aataggtcga
     1021 agcgcagaga tggtcctcca aggcctcctc gcgaattttt ctcgctgtgg ggaccgctcc
     1081 tggaacggtg acgaagatcg tcaccttgac caagtgacgc ttctcgaacc cagccgccct
     1141 cagcacgccg aatagattgc tccaggctcg gcgcatctgc gcctccagcc cctcctcgat
     1201 gctgccgtcc ggcctgtccc cagtttgccc cgaaatgacg agccgttcgc cacccaacgt
     1261 atgaaccacg ccttggacgt agttggaggc aggtttggca atatcgtcag ggttgatgcg
     1321 ccgcatagcg tgctcctcta aagctcttgt cgttatttcg accgctgggt ccgaaccgac
     1381 tgtccagccg tcgcccgcat tatcccagca actgcacggc cgggccagcc actatgccag
     1441 tcacgcatcc gcagcacgct ctcctctggc agggggcatg tgccgtccga gcttccacgc
     1501 gcctcgactg cacctactgc ctgcacattg cgggaacgaa cgaggcttgg acgccgttgc
     1561 cccactgagc tgatctaagg aaacggcatg agcgacacag agcaaacaga agctggacgc
     1621 ccgcccgacg cggcttgggt gaacgtcggc ccgcaagagc ggaccgcgtc tgcgctgctc
     1681 gggtcgcttt tgctcgcctg gggcgcgcgc agaggtcgtc tcatcggaac catcgccgcg
     1741 cttggcgggg ctgctttgct tgcccgcggt gcaacgggcc actgcccggg ttacgcaact
     1801 ctccgcccga cggaggagga aacacgtttc gcccgcgagc gcggctggtc gaccgccgtc
     1861 gtggcccggg aaagcattgt gatcgatcgg cccccggaag agctttaccg cttctggcgc
     1921 gacgagatca acttgcctcg gttcatggag agcctcgagc atgtggaaaa gctttccgac
     1981 cgcagctcgc gttggacggt gctcggtcct gctgggcgcc ggttgcattg ggtctcaaca
     2041 atcaccgagg acagaccatc agaattcata gcctggcgag ccgaagagaa tgccgacatt
     2101 cgcaatgcgg gctgggtgga gttccgcgat ttaggaagcg ggacgagcac acgagtggaa
     2161 accttcatgg cctacgagcc gcctatcggt ctggctggcc acgcaatcgc cagcttgcta
     2221 gggcgcgacc ctgcgagcgc gatgcggcgc agcctgcagc ggcttaagca ggtggtagaa
     2281 agtgagatgg aacaggaaaa caaacagccg acaggagctg agcagtactg atcagcgctg
     2341 ctagcagaag ctgtttggcc gcttcccctc ccgctgggcg tgtcgacgca ttgcgatcaa
     2401 gggccgccta tactatctcc tccagggatt gtgctgccga aaggcagtga tctggaacca
     2461 atcccaggca gacgcgtcct ctcgcgaggc attagcgctc gtgagctagg gatgcgcgtt
     2521 tgccaggccc accttataac aaagcttgca tgactctccc ggacctggat ttcgaaaagg
     2581 atgccctctt tctggatttg gacggcacct tgatcgacat tgctccgcat ccagatgatg
     2641 tcgtcgctcc gccagacctc atccgctctc ttggcgctct cgagcggcgg ctgggcgggg
     2701 cgctttcggt catatcaggc cggccgatcg ctgccatcga cgccctgctc gcgccgctac
     2761 gtctccccgc atccggtgtg catggtgccg aaatccggtt cgagggagga caggcgaaca
     2821 tcgcgcgagc cgcgccaccc atccccaacg gagttcgatg gctgctggcc cctttggccg
     2881 aagttcctgg cgtgctcatt gaagacaaga cgatcgccat agcggtccac tttcgccagg
     2941 cgcctgccgc cgccgagcgc gtcagggaag tagtggaagc tgccgtttcc gcccatcgag
     3001 ccgatcagct caccatcgtg cctgggaagt gcgttctcga gatcaagcat gcacgattca
     3061 gcaagggcac tgccgtcgaa gagttcatgg cacgcccacc ctgggctggc cggcgaccgg
     3121 tgttcatcgg ggatgatgtt acggacgaag cggcattcgc cgtgttgccc aaatggaagg
     3181 gcgtgggaat agccgttgga cagcctcgac caggtgctgt cgcaggcttc gccactgcat
     3241 ctgaggttcg caattggctg gcgggtcttg cccgcgagga gtgcacgacg tgagcgcacc
     3301 gggtctcgac tacggtctca taggtaacgg ccgcatcgcg gctttgatcg acaagaacgg
     3361 gcgtatcgtc tggtggtgct tcccgcgcct tgatggcaac cctgtattct cacggctcct
     3421 cgccggagac gaggacaagg gatacacgga cgtcgtcc
//
LOCUS       DASUMG010000160         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_261588, whole genome shotgun sequence.
ACCESSION   DASUMG010000160 DASUMG010000000
VERSION     DASUMG010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261588"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VFX46_02595"
     CDS             <1..840
                     /locus_tag="VFX46_02595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961301.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5599504.1"
                     /translation="VAATASDPAPMQYLAPFAGCTMGEFFRDNGMHAVIAYDDLSKQA
                     VAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNEEHGLGSLTALPVIETQAND
                     VSAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSAAQTKAMKQVAGRIK
                     GELAQYREMAAFAQFGSDLDATTQRLLARGARLTELLKQPQFSPLKMEEQVVVIFAGT
                     RGYLDPLPVNAVGRFEQELLRIMRDQHADILETIRTEKEISSETEKKLVEAIDQFAKA
                     FTP"
     gene            1044..1892
                     /locus_tag="VFX46_02600"
     CDS             1044..1892
                     /locus_tag="VFX46_02600"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009466634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX5599505.1"
                     /translation="MASLKDLRNRIASVKATRKITKAMQMVAAAKLRRAQDAATAARP
                     YAERMDSILANLNRRITSREGLSPLLVGTGRDQTHLLVVMTAERGLCGGFNANIARLA
                     RQDIQRLQQEGKTVKILSVGRKGYDILRRDYGNLVVDRIDLRSVRQIGYAHAQEIANK
                     ILALFEAGEFDVCTLYFSEFKSVITQKPTALQLIPAKLPDEESSEAHTGAVAVHEYEP
                     GEEEILEYLLMRNLSTQVFRGLLENAASEQGARMTAMDNASRNAGEMIDRLTLTYNRT
                     RQAMIT"
     gene            2037..>2536
                     /locus_tag="VFX46_02605"
     CDS             2037..>2536
                     /locus_tag="VFX46_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX5599506.1"
                     /translation="MASNKVGRIRQVMGAVVDVQFEDELPDILNALETNNHGNRLVLE
                     VAQHLGENTVRTIAMDSTDGLVRGQEVTDTGRPIAVPVGEATLGRIMNVIGEPVDEAG
                     PIVGVEARPIHQPAPEYVEQSTEAQILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTV
                     LIQELI"
BASE COUNT          518 a          814 c          775 g          429 t
ORIGIN      
        1 gtggctgcga cggcctccga tccggcgccg atgcaatatc ttgcgccgtt cgccggctgc
       61 acgatgggtg agttcttccg cgacaacggc atgcacgccg tgatcgcgta cgacgacctt
      121 tccaagcagg ccgtggccta ccgtcagatg tcgctgctgc tgcgccgtcc gccaggacgc
      181 gaagcctatc cgggtgacgt cttctacctg cactcgcgct tgctggagcg cgcggcgaag
      241 ctcaacgaag agcacggttt gggctcgctg accgccctgc ccgtcatcga gacgcaggcc
      301 aacgacgtgt cggcctacat tccgacgaac gtgatttcca tcaccgacgg ccagatcttc
      361 cttgagacgg acctgttcta tcagggcatc cgtcctgcgg tgaacgtcgg tctttcagtg
      421 tcgcgcgtcg gctcggccgc gcaaacgaag gccatgaagc aagttgccgg gcgcatcaaa
      481 ggtgagctcg cccaataccg cgagatggcg gccttcgcgc agttcggctc cgacctcgac
      541 gccaccacgc agcgccttct tgcccgcggt gcccgcctga ccgagctgct gaagcagccg
      601 cagttctcac cgctcaaaat ggaagagcaa gtggtcgtga tctttgccgg tacccgcggc
      661 tacctcgatc cgctgcccgt caacgcggtc ggccgcttcg agcaggagct gctgcgtatc
      721 atgcgcgacc agcacgcgga tatcctcgag accatccgga ccgagaagga gatttcctcc
      781 gagacggaga agaagctcgt cgaggcgatc gaccagttcg ccaaagcgtt cacgccttag
      841 ccttcgtgtc gttctggccc gggagagcga gaatcgatcg cagatccgag gccagagaag
      901 ggaagcgccg aaggcttctt ggctcaagag tggtaggccg acgccttcgg cagagttctt
      961 ctaggtctct aggtctcgaa tttccgctcg cctttgtgac caccagcccg aaggcgactg
     1021 actaagaagg aagacacggc ccgatggcca gcttgaagga cctgagaaat cggatcgcgt
     1081 cggttaaggc gacgcgtaag atcaccaagg ccatgcagat ggtcgccgcg gccaagttgc
     1141 gccgggcgca agatgcagcg accgcagcac gcccctatgc tgagcgcatg gacagcattc
     1201 tggccaacct caaccggcgg atcaccagcc gagaaggcct ttccccgctc ctcgtcggca
     1261 cgggtcgcga ccagacacat ctgctcgttg ttatgacggc cgagcgcggc ctttgcggtg
     1321 gcttcaacgc caacatcgcc cgactggctc ggcaggacat ccagcgcctg cagcaggaag
     1381 gcaagacggt caagattctc agcgtcggcc ggaaaggcta tgacatcctg cgccgggact
     1441 acggcaacct ggttgtcgac aggatcgatc tccggagcgt gcggcagatc ggctatgcac
     1501 atgcccagga gatcgcaaac aagattctcg ccctcttcga ggcgggagag ttcgacgtct
     1561 gcacgctcta cttcagcgag ttcaagtccg ttatcacgca aaagccgacg gccttgcagc
     1621 tgatcccagc caagctgcct gacgaggaat cgagcgaagc acacaccggg gctgtggctg
     1681 ttcacgagta tgagcccggc gaggaggaaa tcctcgaata cctgctgatg cgcaatctct
     1741 cgacgcaggt cttccgcggt ctgctcgaga acgcagcctc cgagcagggc gcgcgcatga
     1801 ccgccatgga caatgcctcg cgcaatgccg gcgagatgat cgacaggctg accctgacgt
     1861 acaaccgcac gcgccaggcc atgatcacct aggagctgat cgagatcatc tcgggtgccg
     1921 aggcgctgta gtgactagat cagcatggat aggctgaatg aaggaatttc gacgatcgag
     1981 caccgtctcg atctggtcaa agcagactga aaccgccgca cgcacaggga atgccaatgg
     2041 ctagcaacaa agtgggtcgc atccgccagg tgatgggcgc cgtcgtcgac gtgcaattcg
     2101 aggacgagct gcccgacatc ctcaacgcgc tggaaacgaa caatcacggc aaccgcctcg
     2161 tgctcgaggt cgcccagcat ctgggtgaga acacggtccg caccatcgcc atggactcaa
     2221 ccgacggcct ggtccgcggc caggaggtca ccgacacggg tcgcccgatc gccgtccccg
     2281 tcggcgaggc cactctcggc cggatcatga acgtgatcgg cgagcccgtg gacgaggccg
     2341 gtccgatcgt aggcgtcgag gcccgaccca ttcaccagcc ggctcccgag tacgtcgagc
     2401 agtcgacgga agcgcagatc ctcgtcacgg gcatcaaggt tgtcgacctg ctcgcaccat
     2461 acgccaaggg cggcaagatc ggcctgttcg gcggcgccgg cgtcggcaag accgttctca
     2521 tccaggaact gatcaa
//
LOCUS       DASUMG010000161         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1064758, whole genome shotgun sequence.
ACCESSION   DASUMG010000161 DASUMG010000000
VERSION     DASUMG010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064758"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..576
                     /gene="rplQ"
                     /locus_tag="VFX46_02610"
     CDS             142..576
                     /gene="rplQ"
                     /locus_tag="VFX46_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX5599507.1"
                     /translation="MRHRKLGRKFSRDSGHRQAMLSNMAAALIRHEQIITTLPKAKDL
                     RRVVDRYITLAKRGDLNSRRLAAARLRDEEMVKKLFEVLAPRYKDRNGGYTRVLKAGY
                     RFGDSAPRAVIELVDRDVSAKGKEDKARVAASEQEQEAQAAS"
     gene            607..1629
                     /locus_tag="VFX46_02615"
     CDS             607..1629
                     /locus_tag="VFX46_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEX5599508.1"
                     /translation="MGSSVLVTGATGFIAKHCIAELIRQGYRVRGTARHPDARTRVVN
                     ALARAGIDASHLEICKADLLHDDGWDAATEGCSHVLHVASPFPVKPPRNRDEVVRPAV
                     DGTLRVLKAAARAGVQRVVLTSSMVTCIYPSGGKQGRTYTEEDWTDTDRRDISSYVFS
                     KVLAERAAWEFVQQTPGAPEITVLNPGLVYGPALDLDLSTSHQILTLMAKGKYPVVPR
                     AGYSVVDVRDVAAAHVSAMTHPAAPGERFLVTSDYVTLVDIALMLAHALPDVASRIPR
                     REAPDILIRGLSALDRSLLAVIPDLSVRRECNNAKARERLGIAFRPAREAIRAAAESL
                     RELGAI"
     gene            1845..>2275
                     /locus_tag="VFX46_02620"
     CDS             1845..>2275
                     /locus_tag="VFX46_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX5599509.1"
                     /translation="MAEAQVRVPPPSREAIQYSYAPIVKRAAPAVVNVYVRSRVRAGI
                     PPLFDDPFFRRFFGDGFGMPRERIQNSLGSGVIVSPDGIVVTNYHVIRGGESAEIRVA
                     LADRREFDAKVILQDEKTDIAVLRIEANGETFPTLELADSDA"
BASE COUNT          427 a          682 c          717 g          449 t
ORIGIN      
        1 ggttcgagga tcagtattga ttcgcgaatg gtgagtagcg aatagcgaat gggagccaac
       61 ctgttcgcta tttctctact ggctcacaag aggtgaggcc aaagaacctc cccgaaacaa
      121 ccacatcaaa ggacgaagac gatgagacac cggaagctcg gccggaagtt cagccgggat
      181 agtgggcatc gccaggcgat gctctccaac atggcagccg ctcttatccg ccacgagcaa
      241 atcattacga cgctgcccaa ggccaaggac ctgcggcgcg tggtggatcg gtacatcacg
      301 ctcgccaagc ggggcgacct caattctcgg cggctggctg cagcccggct gcgggacgag
      361 gagatggtca agaagctttt cgaggttctc gctccgcgct acaaggacag gaacggcggc
      421 tacacccgtg tgctcaaggc cggttaccgt ttcggcgata gtgcaccccg cgcggtgatc
      481 gagctggtcg atcgggacgt cagcgccaag gggaaagagg acaaggcccg cgtcgctgca
      541 agcgagcagg agcaggaggc gcaggcggca agctgatccg cccgccagtt tccagggggg
      601 tgaagcatgg ggtcctcagt tcttgtcacc ggggccacag gcttcatcgc caagcactgc
      661 atcgcagagc tcatccggca aggatatcga gttcgtggaa cagcgcgtca cccggatgcc
      721 cggaccaggg tcgtaaacgc tttggcacgg gccgggattg atgcgtccca ccttgaaatc
      781 tgcaaagccg atctcctgca cgatgatggt tgggatgcag ctacggaagg ctgctcccat
      841 gtgctccacg tcgcctcacc atttcctgtg aagccaccgc gcaaccgtga cgaggtcgtg
      901 cggccggctg tggacggtac gttgcgtgtc ctgaaggcgg ccgctcgtgc cggcgtgcag
      961 cgagtggttc tgacctcctc catggtgacc tgcatctatc cttccggggg aaagcagggg
     1021 cgcacgtata cggaggaaga ttggaccgac accgatcggc gtgacatcag ttcctacgtc
     1081 ttctccaaag tgttggctga aagggcggcg tgggaattcg tgcaacaaac cccgggcgcc
     1141 ccggagatca cggttctcaa tcccggcctg gtttacgggc cggcgctcga tctcgatcta
     1201 tcgacttccc atcaaatcct gactttgatg gcgaagggga aatacccggt cgttccacgc
     1261 gcggggtact ctgtcgtcga cgtacgcgat gttgcggcgg cccacgtcag tgcgatgact
     1321 catccggctg cgccaggaga acggttcctc gttacgagcg actacgtcac gctggttgat
     1381 attgcgctta tgcttgccca tgcgctacct gacgtggcct cgcgcatccc gaggcgggag
     1441 gcgccggaca tcctcattcg cgggctttcg gcgctcgacc gcagtctact cgccgtcatc
     1501 cccgacctct ctgtccggcg cgagtgcaat aacgcgaagg cgagagagcg gctcggaatt
     1561 gcgttccgtc cggcgcgcga ggccatcagg gcggcagcag agtcgctgcg cgagctgggt
     1621 gcaatctaac atagcctcat gagtttcgtt gggcttggct tctatgcctt ggggcgaagc
     1681 gtctattatc tgttgcgtgg tcgaaatcgc agcccatggt tgcagcgctc gataagaacc
     1741 acgcgaagtg ggggctttca atgacacatc gccatcggtc cgtttggccg cggatggcag
     1801 ttctggctct tgcagttctg ccgagcttct tagtgagctc gcacatggcc gaagcacagg
     1861 tacgcgtgcc gccgccctcg cgcgaagcca ttcagtattc ctatgcgccc attgtgaagc
     1921 gcgccgctcc ggccgtcgtc aacgtctatg tgcggagccg tgtgcgagcc ggcattccgc
     1981 cgttgttcga cgaccccttc ttccgccgct tcttcggcga tgggttcggc atgccgcgag
     2041 agcggatcca aaactcgttg ggctcgggcg tgatcgtcag cccggatggc atcgtcgtca
     2101 ccaattatca cgtcatccgc ggcggcgagt ccgcagagat tcgtgttgcc ttggctgacc
     2161 gtcgggagtt cgacgccaag gtcatccttc aggatgagaa gaccgacatc gccgtgctgc
     2221 ggatcgaggc taacggcgag acatttccca ccttggagct tgccgactcc gatgc
//
LOCUS       DASUMG010000162         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_823660, whole genome shotgun sequence.
ACCESSION   DASUMG010000162 DASUMG010000000
VERSION     DASUMG010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823660"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..149
                     /locus_tag="VFX46_02625"
                     /pseudo
     CDS             1..149
                     /locus_tag="VFX46_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(658..1017)
                     /locus_tag="VFX46_02630"
                     /pseudo
     CDS             complement(658..1017)
                     /locus_tag="VFX46_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1317..2675)
                     /locus_tag="VFX46_02635"
     CDS             complement(1317..2675)
                     /locus_tag="VFX46_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007006989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5599510.1"
                     /translation="MNSIRSRLLGILIGTTLFVWLAAIAWIYTSTQAEVERVLDARLT
                     EAARMVNSLLADHRIEIASQGNVATLTTLANLAAAKAYERQLSCQIWSLDGTLVARSE
                     SAPELPLTNAMQGFSEDIVDGETWRVFTIENAELGVRVMVGDNLRMRERLVGDVVKGM
                     VHSAALAIPFLVGMIWLSVSRGLMPLSDMARALSQRSANELTPLPSAGIPKEIAPAIE
                     AINDLFGRVEAARERERSFTAFAAHELKTPLAGLKTQAQVALSSTDGAVHANALRQIA
                     AGVDRTSRLVKQLLDMAALEASDARPCHSYESMNHLVSSVVADLLPLASARGIPIKLE
                     SSEQDVRAKVEPHFFVLALRNLLENALDHANDVVTCQVLDLGKEVRVVIEDDGPGIPE
                     PELELVTAKFVRGRHKSATGSGLGLAIAKVAMARIGGRLTLHNRKPTGLAATLEIPNR
                     AA"
     gene            complement(2672..3337)
                     /locus_tag="VFX46_02640"
     CDS             complement(2672..3337)
                     /locus_tag="VFX46_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599511.1"
                     /translation="MRILIVEDDPLLLDGLAVGLGLKGFTVDAVANCEDGRAAVRAQR
                     YDGIVLDVMLPDGSGFDLLKELRDAKDGTPVLLLTARDQVPERIAGLDAGADDYVGKP
                     FDLDELAARLRAIARRGAGRATAHLEWRGVVLDPASLTATRDGVPLKLTRREFAALRA
                     LMERPAATVSKAALEEALYGWQEEVESNAVEVHIHHLRAKLGQDFIKTVRGLGYRLAE
                     GGA"
BASE COUNT          650 a         1102 c          987 g          685 t
ORIGIN      
        1 agcgcatgaa gaaacgggag gcggaacttg cggccgaggt cggccgttgg tttgaagccg
       61 ccgaggccgc cgatgcgcag gaggacaagc tctacggcga caggcgcggc gatgagttgc
      121 ctgattgggt ggctttgcat cgacagcgat ttgcgcattg tagccctgga tataaccgtc
      181 cttggtcagg agaatgcggc tgtccgcatc ggtgaagtta cgttgcgctt tgccgttggg
      241 ctcggcggac ggcggcgctg gcctcttgcc gttcttcttg cgaccttcgg tgatgcgcct
      301 ttcctcggcc tcggcccggc gccgcatctc ctcttgggcg gcggctttcg cttccgcctc
      361 cagctcggcc ttggcttggc ggatcctctc aagccgcttc tgcttgtcgg ccacccaatc
      421 aggcaactca tcgccgcgct tgtcgccgta gagcttgtcc tcctgcgcat cggcggcctc
      481 ggcggcttca aaccaacggc cgacctcggc cgcaagttcc gcttcccgtt tcttcatgcg
      541 atcataactc atcgccttgt gcttggacgc gttcgccttg atcttggtgc cgtcaagcgc
      601 cacatgcccg agcttcacca aaccggcttt ctcggcaagc tgcaacacct gcacgaacag
      661 agcggccagc gctttgagat gccgccggcg gaactccgag atcgtgcgga agtccggcgg
      721 gtcgcccgcc acgatcatca tgaagtccgc acgctccacc gccgccttcg cgatccgccg
      781 cgaggagtag atgccgctcg cgtaggcatg caagagcagc gccgtcatca tccgcggatc
      841 gaacggcggc tggccgagcg cgctccggta acttccgaca atcccggaaa gatcaagcgc
      901 ttccctcacg agcgccacga tcagccgaga cagatgagcc ttgggcacat agtcctcaac
      961 gcaaggcggc agaagctgtg cgtcgtcgat cttccagggg cgaaaatcct tgctcataag
     1021 tcggcaagga atcagactcg ccgtctcttg accagtgatt actcagacag gctcctagtc
     1081 cagaggatgt cgtaggcttc caccgccgac ctaagcaacg cgttcaggtc gccacacatg
     1141 ccgacttcgt tggttcggtg ctgccgctcg atgaggggat gcggatactt ggtggtagtt
     1201 tcttaaagct gccagccgct ccggagaccc cattgcccag taacaactcc tgatcggagc
     1261 tgaattagtc cggtcatctg gtttgcagct cgcgctttgg gattgtgtct gcctggtcac
     1321 gcagctcgat tagggatttc aagcgtagca gcaaggccag taggtttccg attatgaagc
     1381 gtgagcctgc cgccgatgcg ggccatggcg accttcgcaa tcgccaaacc gaggccactt
     1441 ccggttgctg atttgtggcg gccacggacg aatttagcgg ttactaattc cagttccggc
     1501 tcgggaatac ctgggccatc gtcttcgatg acgacccgga cctccttccc caggtcgagc
     1561 acctggcacg tcaccacgtc attcgcgtgg tccagtgcgt tctcgagcag attgcggagc
     1621 gcgagaacga aaaagtgcgg ttcgactttg gcccgcacgt cctgttccga gctctcgagt
     1681 tttatcggaa tacctcgcgc ggatgccaag ggaagcaaat ccgcgaccac ggagctcaca
     1741 agatggttca tactttcata cgaatggcat ggtcgcgcat cgctcgcctc gagtgctgcc
     1801 atatccagca gctgtttgac caaccggctc gtccggtcaa caccggccgc aatctgccgg
     1861 agtgcattgg cgtgaacagc cccatccgtg ctcgacagcg cgacttgagc ctgggtcttg
     1921 aggcccgcaa gcggcgtttt caattcgtgc gctgcaaagg ccgtaaaact ccgttcccgt
     1981 tcacgagccg cctccacccg tccgaaaagg tcgttgattg cctcaatagc cggagcgatt
     2041 tccttgggaa ttccggcact cggtaaaggc gtgagctcgt tcgccgatcg ttgcgagagc
     2101 gcgcgcgcca tatcgctcag tggcatgagc ccgcggctga cactcagcca aatcatcccg
     2161 acgagaaaag gtatggccag ggccgccgaa tgtaccatcc ccttcacgac atcgccgacg
     2221 agccgctcgc gcatccgcag attatcgccc accatgacac gaacgccgag ctcagcattt
     2281 tcaattgtga aaacacgcca agtctcgcca tcaacgatgt cttccgaaaa gccctgcatc
     2341 gcattcgtca gcggcagctc gggggcactt tcggatcgag caacgagcgt cccatcgagc
     2401 gaccaaatct ggcaggagag ttgccgctca taagccttcg ctgcggcaag atttgctagg
     2461 gtggtcaacg tggcgacatt cccctgagaa gctatctcga tccggtgatc tgccagcagc
     2521 gagttcacca tgcgcgctgc ttcggtcaga cgtgcatcga gcactcgctc cacctcggcc
     2581 tgcgtgctcg tgtagatcca agcgatggcc gcaagccaca cgaacaacgt ggtaccgatg
     2641 agaattccga ggagccggct acgaatggaa ttcacgcgcc tccctccgca agtctataac
     2701 ccagcccacg gaccgtcttg atgaaatcct gtcccagctt ggcccgcaga tgatggatat
     2761 gaacctcgac cgcgttgctt tcgacctcct cctgccaacc gtatagcgcc tcctccaggg
     2821 ccgccttcga gacagtggcc gcggggcgtt ccatgagggc gcgaagcgcg gcgaattcgc
     2881 gccgcgtgag cttcaggggt acaccgtctc tcgttgccgt cagagacgct ggatcgagca
     2941 ccacaccccg ccattcgaga tgcgcagtgg cacgcccggc gccgcggcga gcaatggcgc
     3001 gcagtcgtgc agccagctcg tcgagatcga atggtttgcc gacgtagtcg tctgcgccgg
     3061 cgtcgagacc cgcaatgcgt tccggcacct ggtcgcgtgc cgtgaggaga agcactggcg
     3121 tgccgtcttt ggcatcgcgg agttctttca ggagatcgaa ccccgaaccg tcgggaagca
     3181 tgacgtcgag cacgatgcca tcatatcgct gcgcgcgaac agcggcgcgg ccgtcctcgc
     3241 agttagcgac ggcatctacc gtaaacccct tgagcccaag cccgacggct aacccgtcga
     3301 gcaacagagg atcgtcttct acaatcagta tccgcatgat gtgtcatccc gtacgcacct
     3361 cgctggtgtc attcgccgct taaggctgcc ttaagctccc agcgacacct ccgcctccag
     3421 gcac
//
LOCUS       DASUMG010000163         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_823702, whole genome shotgun sequence.
ACCESSION   DASUMG010000163 DASUMG010000000
VERSION     DASUMG010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823702"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1220)
                     /locus_tag="VFX46_02645"
     CDS             complement(15..1220)
                     /locus_tag="VFX46_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX5599512.1"
                     /translation="MGSLQLMTWFIALAILPAALAGPAAAQTCTKQDIAAVIDSTAEK
                     LRKLNAAHQPELQAKLRQLGDRRGWSDEEREAKAAEFLDDDETRALDEQASKLLIELD
                     VIGDEAEIDPSTCQTRLEKLKGISTQLIEITTAKAAHASARIDAELNALSSSDRSRTA
                     EQPGKAAPRMATQTEPSPKSQPMSAAPALTPPKTRDARPMPPAEGGWDTDTVRHAAID
                     EADHVPPPEVLADLPPYVHQSEELEFTVEEIRAAGRGFFGSLSAGLASVIEYAFQTYG
                     RPNGYILGSEGGAALLAGLRYGDGRLVTKLGGERKVYWQGPSVGYDVGLAGSRVMFLV
                     YNLERPEDLFARFMGVDGSAYVVGGVGMTFLKKGRVVLAPIRTGLGLRVGANVGYLKF
                     TPERSLNPF"
     gene            1360..1725
                     /locus_tag="VFX46_02650"
     CDS             1360..1725
                     /locus_tag="VFX46_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599513.1"
                     /translation="MMMTRYLLAAIALIILTGPLASAAQALGLDFLHAQARVGNKVCM
                     TEHEHYGEGTMPTKQAAQSVAIRSWSDFTAFEYGRIWGSYRLAEGKRMDCSQTANGWL
                     CKTYARPCRPAKYGPPRRR"
     gene            1872..2390
                     /locus_tag="VFX46_02655"
     CDS             1872..2390
                     /locus_tag="VFX46_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX5599514.1"
                     /translation="MLTLSLLRHGQSSWDDPDLNDHERPLSKHGRNAAEIISDYIAEH
                     GLVPDHILCSTAVRARETLDVLLTRLGLLQPEIALEPALYLATPTALIGIIRETARSV
                     RHLMIIGHNPGLHALALDLTGSGLRRDITSMAVKFPSGALAVLTFEENDWTEIRPAAG
                     RLVQFVATRSVA"
     gene            2423..3037
                     /locus_tag="VFX46_02660"
     CDS             2423..3037
                     /locus_tag="VFX46_02660"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX5599515.1"
                     /translation="MLVLIDNYDSFTYNLVHFLGELGAACEVHRNDKITVDEVLRSKP
                     KAIVLSPGPCTPNEAGICLELVDAAARADLPMLGVCLGHQAIAQAFGGKIVRAPVPMH
                     GKLSVVRHQGEGIFKGLPSRFNATRYHSLIVERASLPNCLHVTAETPDGIIMGLQHKT
                     HPIHGVQFHPESIASECGHDLLANFLTLAGLSPRRRNVRSAKAA"
     gene            3069..>3771
                     /gene="trpD"
                     /locus_tag="VFX46_02665"
     CDS             3069..>3771
                     /gene="trpD"
                     /locus_tag="VFX46_02665"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX5599516.1"
                     /translation="MSTNELRHLIQKVATGATLNSEEIRTALETMTAGLATPAQMGAF
                     LMALRVRGETVEEITGAAQLMRSKMTRVEAPEDTVDIVGTGGDGQGTYNVSTCASLVA
                     AGAGMHIGKHGNRAVSSKAGASDVLAALGVKLDVPPPIITDCIIKAGVGFMWAPMHHS
                     AMKHWAPVRAELGIRNIFNLLGPISNPAGVKRQVVGVFAAQWVEPIAHVLKNLGSEHV
                     WVVHGHDGLDELTTTG"
BASE COUNT          725 a         1214 c         1123 g          709 t
ORIGIN      
        1 gcctcggtcc tttgtcaaaa tggattgagg ctgcgttccg gtgtgaactt cagataaccg
       61 acattggcgc cgacccgcag gcccagtccc gttcggattg gcgccagcac gacgcggccc
      121 tttttcagga aggtcatacc gacgccgccg acgacgtagg ccgaaccatc cacgcccatg
      181 aagcgggcga agaggtcttc gggacgttcc aggttgtaga cgaggaacat gacccgggag
      241 cccgcgaggc cgacatcgta cccgaccgac ggaccctgcc agtagacctt ccgctcgcca
      301 ccaagcttcg taaccaaccg tccatcgccg taccgaaggc ccgcaagcaa ggccgctccg
      361 ccttcggacc cgaggatgta cccattgggt cgtccataag tctggaatgc gtactcgatg
      421 acgctggcaa ggccggccga aagcgaaccg aagaatccgc ggcctgcagc gcgaatttct
      481 tcgaccgtga actccagctc ctccgactga tgcacgtacg gcgggaggtc ggccaagacc
      541 tcgggaggag gaacatggtc cgcctcatca atcgctgcat gacgaaccgt gtcagtgtcc
      601 caaccgccct ccgcaggcgg catcggacga gcatccctgg tcttcggtgg ggtaagtgcc
      661 ggcgcagcac tcattggttg agatttcggc gacggctccg tttgagtagc cattctcggt
      721 gccgctttgc caggctgctc tgcagtgcgg ctgcggtccg acgaactcag cgcgttcaac
      781 tctgcgtcga tccgtgccga ggcatgcgcg gccttggccg tcgtaatctc gatgagctga
      841 gtggaaatcc cctttagctt ttccagcctg gtctggcatg tgctcggatc gatttccgcc
      901 tcgtcgccga taacgtcgag ctcaatcagg agcttgctcg cctgctcatc gagcgcccgc
      961 gtctcatcgt catccagaaa ctcggctgcc ttggcttcgc gctcctcgtc gctccaaccg
     1021 cggcgatcac cgagctgcct cagtttggcc tgtaactccg gctggtgagc cgcgttgagc
     1081 tttctcagct tctccgcagt ggagtcgatg acggcagcaa tgtcttgctt ggtgcaggtc
     1141 tgagcggcag ccggcccggc caatgcggcg ggcaagatcg ccagcgcaat gaaccatgtc
     1201 atcagctgca atgaacccat gaagtaatcg tccaagtttg cttatgcgtt cggcagcgta
     1261 ggtccaggta agggaaactt tgccatagac aacggtcgtc cgtgcggcaa ccggaggtgg
     1321 cgttatcatc tgcctgaata tcacaacgaa aaaggaggca tgatgatgac ccgatacctg
     1381 ctcgcggcta ttgctttgat aatactcact gggccgcttg catcagctgc acaggcattg
     1441 ggactggatt tccttcatgc acaagctcga gtcggcaaca aggtctgcat gaccgagcac
     1501 gagcactatg gcgaaggcac gatgccaacc aaacaagccg cgcagagcgt tgcgatccgc
     1561 tcctggagcg attttaccgc cttcgagtac ggcagaattt ggggcagcta tcgattggct
     1621 gaaggcaagc ggatggattg ctctcagaca gcaaacggct ggctttgcaa gacgtacgca
     1681 cgcccgtgcc gtccggcgaa gtacggcccg ccgcggcggc ggtgacatca accgacataa
     1741 gctaatcagt cccattaaga cgacccgggg tcccaggccg tctacctgac ttcattcgat
     1801 tccggggagc acagagcata gaaatgtggt aggctcatcg aacgtgcctc ggggcacggc
     1861 ggagatgccc catgttgacg ctgtcgctcc tccgccatgg tcaatcatcg tgggacgatc
     1921 cggatctcaa cgatcacgaa cggccgctct caaagcatgg caggaacgcg gcggagatca
     1981 taagtgatta catcgcagag cacggcctcg tgccggatca catcctctgc tcgactgccg
     2041 ttcgcgcccg cgaaacactc gatgtgctcc tgacccggct gggtttactg cagccggaga
     2101 tcgctctgga gccagccctc tatctcgcga cgccgaccgc gctgatcggc attatccgcg
     2161 aaaccgcgag gtccgtgcgc cacttgatga tcatcggtca caaccccggc ctgcacgccc
     2221 tcgctcttga cctgaccggc tctgggctgc gccgcgacat tacgtcaatg gccgtaaagt
     2281 ttccgtccgg cgccctcgcc gtgctgacat tcgaagaaaa cgactggacc gaaatccgtc
     2341 ctgcggcagg tcggctcgtc caattcgtcg ctacccgcag cgtcgcttga cgaattccgc
     2401 tcacgtctcc tacacgttca ccatgctggt cctcatcgat aactacgaca gcttcaccta
     2461 caatctcgtc catttcctgg gcgagctcgg cgcagcgtgc gaagtccatc gcaacgacaa
     2521 gatcacggtg gacgaggtgc tccggtcgaa gccgaaggcg atcgtgctgt cgcccggacc
     2581 gtgtacgccg aacgaggctg gcatctgttt ggagctcgtg gacgcagcgg cccgcgccga
     2641 cctgccgatg ctcggcgttt gccttggtca tcaagccatt gcgcaggctt tcggtggcaa
     2701 gatcgtccgc gcgcctgtgc cgatgcacgg caagctctcc gttgtgcgcc accagggcga
     2761 gggcatcttc aaggggctgc cgagccggtt caatgcaacg cgctatcact ccctcatcgt
     2821 ggagcgcgcg agtctgccca actgccttca cgtcacggcg gaaacgcccg acggcatcat
     2881 catgggcttg cagcacaaga cccatccgat ccacggagtt cagttccacc ctgagagcat
     2941 cgcgtccgag tgcggacacg acctgctcgc caacttcctg actctagccg ggctcagccc
     3001 gcgacggcgc aacgtgcggt ccgccaaggc cgcctgacct tatcgaagct cgagtacaga
     3061 gaacaccgat gtcgaccaac gaattgaggc atctgatcca aaaggtggcc acaggcgcca
     3121 ccctcaattc cgaagagatc cgcaccgcac tcgagacgat gacggcgggc cttgcgacgc
     3181 ctgcgcagat gggcgccttc ctgatggcgt tgcgcgtgcg cggcgagacg gtcgaggaga
     3241 tcaccggcgc ggcccagttg atgcgctcga agatgacccg cgtcgaagcg ccggaggaca
     3301 cagtcgacat tgttggcacg ggcggcgacg gtcagggtac gtacaatgtc tcgacctgcg
     3361 cctccctcgt tgcggcaggt gcgggcatgc acatcggcaa gcatggcaac cgggccgtgt
     3421 cctccaaggc cggagcgtcc gacgtgctgg cggcgcttgg cgtcaagctc gacgtgcccc
     3481 ctcccatcat caccgattgc atcatcaagg ccggggtcgg cttcatgtgg gcgccgatgc
     3541 accattcggc catgaagcat tgggcgccag ttcgggctga gctcggcatc cgcaacatct
     3601 tcaacttgct cggcccgatc tcgaaccccg ccggcgtgaa gcggcaggtt gtgggcgtct
     3661 tcgcggcgca gtgggtcgag ccgatcgcgc acgtgctgaa gaacctgggg tccgagcacg
     3721 tctgggtcgt ccacggccac gacggcctcg acgagctgac cacgaccggc t
//
LOCUS       DASUMG010000164         2739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_503294, whole genome shotgun sequence.
ACCESSION   DASUMG010000164 DASUMG010000000
VERSION     DASUMG010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503294"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(641..1384)
                     /locus_tag="VFX46_02670"
     CDS             complement(641..1384)
                     /locus_tag="VFX46_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX5599517.1"
                     /translation="MLMKRPIRPSILLAAILSLLMTVPATAQRSAPLGQHEAGVFDYY
                     TLVLSWSPTYCASVRSPDNDPQCRRTARPYAFVLHGLWPQYERGFPEYCPTRERPFVP
                     QRIIDQMLDIMPSPRLVIHEYRKHGTCSGLDPAGYYDLSRRLYEKINIPKRFRLPAAP
                     FFISPQQVVDEFIDANPGLKVEMLTVDCRGSGNRLREVRICFSREGHFRPCGSNERRA
                     FCRANRMYVPPVRVGGGAGTTKPQGQRAL"
     gene            complement(1417..>2739)
                     /locus_tag="VFX46_02675"
     CDS             complement(1417..>2739)
                     /locus_tag="VFX46_02675"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX5599518.1"
                     /translation="LLRIKSISTDPAYKDECAAAAEWCARALRQIGFEVSIRETTGHP
                     MVVGHSHREVPKGMPHVLFYGHYDVQPPDPLDLWTSPPFEPRLVEDSRNGTVIVARGA
                     EDNKGQLMTFLEAARAWKQVAGTLPVAITVLLEGEEECGSPSLPQFLEKHAQEITCDV
                     ALVCDTTQWDKDTPGITTMLRGLAGTEVIVTGPNRDLHSGLYGGPAINPIRVLVRMLA
                     ALHDDDGRVRVPGFYDGIAEVPSSQAEQWRRLGFDEKAFLGEVGLSVPAGEKGYTALE
                     QLWARPTCEFNGITGGYQGPGTKTVIPSRASAKITCRLVAGQDPDKITASLQQFLAER
                     VPPDCKVEFVPFHGNAAISFDPNAPYVQAAAQALEEEWGRPAALMGSGASIPIVTAFK
                     THLGMDSLLIGFGTDDDCIHSPNEKYNLTSFRKGTRSWARILQRLARV"
BASE COUNT          534 a          829 c          877 g          499 t
ORIGIN      
        1 taacagcaga ggacccaccg tttggtgacg ggtttaggac gagattatta aaacgtgctg
       61 agctgagcga atgagcgatc cgtatggctc cgcggcttac tccaccatgc cgaggcggtc
      121 ctgacggccg gcgctaaccg cgaacttcaa cggtgggtcg tcagcagtgc acggatgcag
      181 ccgtgtttgc ttgaccactc gatccagcac gagcgtggcg cagtcaggcc aaatgttgct
      241 ggcatctgag agcgaagcgg gcactctgtc cgccggcttc gcagcggcga cggtctggat
      301 cgaagaggtg tgctcgggcg cgaaggtcag cgcgagaact cgcgggtttg gatctaggcc
      361 cgttgcgctc caaaacagtg ttccggcaac aaaaccgaga gcagcccaaa tcgcagccct
      421 gctgcgtgaa ccgcgcttgt cttgcaatag tggtcggccg gtggattgat ccaaggccgg
      481 atcagcatgc cgcgagtttg ccatggaatc cctcgtccga gcgcgaatct gttgcgttcg
      541 ggtaccatga tgccgaacgg ccaccacact gcaacagctg gccggtgctc aagctggcaa
      601 cgtcgcaagt tctccacgga aatctcaacg tgcgcactga tcagagtgca cgctgacctt
      661 gcggcttagt ggtgccagct ccaccgccga ctcgcacagg aggaacatac atgcggttcg
      721 cccggcagaa cgcgcggcgc tcgttgctgc cacaaggccg gaaatgtcct tcacgcgaaa
      781 agcagatccg aacctcgcgc agccggttac ctgagccgcg gcagtcgacg gtcagcattt
      841 cgaccttcag gccgggatta gcgtcgatga actcatcgac gacttgctgt gggctgatga
      901 agaacggcgc ggcgggcaag cgaaatcttt tcggaatgtt gatcttttcg taaagccggc
      961 gcgagagatc ataatagccg gccggatcga gccccgagca ggtgccatgc ttccgatatt
     1021 cgtgaatgac gagccgcggg ctcggcatga tatcgagcat ctggtcgatg atgcgctggg
     1081 gtacgaacgg gcgctcccgt gtcgggcagt actctggaaa cccgcgctcg tattgcggcc
     1141 acaagccatg aagcacgaag gcgtaaggcc gcgccgtccg acggcactgc ggatcgttgt
     1201 ccggtgagcg cacgctcgcg caatacgtcg ggctccagga caatacgagc gtgtagtaat
     1261 cgaaaacgcc ggcctcgtgc tgacctagcg gcgcgctgcg ctgggccgta gccgggacgg
     1321 tcatcagcag ggagaggatc gccgcaagga ggatcgacgg ccggatcgga cgtttcatca
     1381 acatatcgaa gctcgatcgt tggtgctcag acgtgatcaa acgcgcgcaa gccgctggag
     1441 aatgcgggcc cagctgcgcg tgcctttgcg gaagctcgtc agattgtatt tctcattggg
     1501 tgagtgaatg cagtcgtcgt cggtgccgaa accgatcagc aggctatcca tgccgaggtg
     1561 cgtcttgaaa gctgtaacga tagggatcga ggcgccagag cccatcagcg ctgcgggccg
     1621 accccattcc tcctccagtg cttgagcggc ggcctgcacg tacggcgcat tcgggtcgaa
     1681 cgaaatggcg gcattgccgt gaaacggcac gaactcgacc ttgcaatcag gcggcacgcg
     1741 ctcggccagg aactgctgga ggcttgcggt gatcttgtcg ggatcctgcc cagccacgag
     1801 ccggcacgtt atcttcgctg aagcgcgcga ggggatgacc gtcttggttc cgggcccctg
     1861 gtagccgccg gtgataccgt tgaactcgca cgtcgggcgg gcccatagtt gctccaacgc
     1921 ggtgtagccc ttttccccag cgggcacgct gaggccaact tcgccgagga aagccttctc
     1981 atcgaagccc aggcgccgcc actgctccgc ctgactgctg ggcacctcgg cgataccgtc
     2041 atagaagccc ggcaccctca cgcgcccgtc gtcatcgtga agcgccgcga gcatgcggac
     2101 cagaacgcgg atcgggttga tggccggccc accataaagg ccggaatgaa gatcgcgatt
     2161 ggggccggtt acgatgacct cggttccggc gaggccccgc agcatcgtcg tgatgccggg
     2221 agtgtccttg tcccactggg tcgtatcgca caccagagcg acgtcgcagg tgatctcctg
     2281 agcgtgcttc tccagaaatt gagggaggct cggtgagccg cactcttcct caccttcgag
     2341 gagcactgta atggcgacgg gcaacgtgcc agccacctgc ttccatgcac gcgcggcttc
     2401 cagaaaggtc atcagctgac ctttgttatc ctccgcgccg cgcgcgacga tcacggtgcc
     2461 attgcgcgaa tcctcgacca gccgcggctc gaacggcggc gaggtccaca aatcgagcgg
     2521 gtccggcggc tggacgtcat agtggccgta gaagagcacg tgcggcatgc ccttcggaac
     2581 ctcccggtgg gagtggccca cgaccatggg atggccggtc gtctcccgaa tggagacttc
     2641 gaagccgatc tgtcgcagcg cgcgtgcgca ccattccgcc gcagccgcac attcgtcctt
     2701 gtaggcggga tcggtggaaa tgctcttgat acgcagaag
//
LOCUS       DASUMG010000165         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_744137, whole genome shotgun sequence.
ACCESSION   DASUMG010000165 DASUMG010000000
VERSION     DASUMG010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744137"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFX46_02680"
     CDS             <1..750
                     /locus_tag="VFX46_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX5599519.1"
                     /translation="NPAHTAKVRIFTPGAEIPFAGHPTAGAAVLLAELRAPEPSEDLD
                     AIIALEENIGIVRVGVRLRKGEAAFAEFDAPKLPEQSGTLPPVESIAAAVSLLPREIG
                     FANHRPTRFAAGNSFAFIPVASLEAMAKAEVNSQHWATAISGQGLAGAFLYTRETVHT
                     TSSFHARMFAPDLGVPEDPATGSAAVTFAGVIQMFDELPDGHHKRIIEQGFEMGRPSI
                     ISLTLEVAGGQLRNVRIGGSAVRVTEGRVTI"
     gene            771..1259
                     /locus_tag="VFX46_02685"
     CDS             771..1259
                     /locus_tag="VFX46_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599520.1"
                     /translation="MPNSKLAIPAIAICGLLQIHSPQPASAEQVVWFGNRSEDGASLV
                     YGTPNSGYGKIAFSCEAGEDDVHFVYEHKPIRPEEGVQVEVFLAAGGVEIAIPTTGGR
                     LEIDDAFLLEGQTKLDDNLRKILTAGGQLIVTVEDGAEEFPLDGAAEAAKHLLQVCSP
                     NG"
     gene            1622..>2630
                     /locus_tag="VFX46_02690"
     CDS             1622..>2630
                     /locus_tag="VFX46_02690"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX5599521.1"
                     /translation="MSQTTNSGDRVLIFDTTLRDGEQCPGATMTFEEKLEVADLLDEM
                     GVDIIEAGFPIASDGDFEAVSAIAKRVKRAVVAGLARASFQDIDRCAEAVRHAQRPRI
                     HTFISTSPIHMKYKLQKEPHEVLEMVRASVERARKYVDDVEWSAEDGTRTEIDFLCRC
                     VETAIKAGATTINIPDTVGYTVPEEYYNLFKTVRERVPNSDKAVFSVHCHDDLGMAVA
                     NSLAGVKAGARQVECTINGIGERAGNAALEEIVMAIKTRNDVLPFWTGIETRMLTRAS
                     KLVSAATSFPVQYNKAIVGRNAFAHESGIHQDGMLKHTQTYEIMTPESVGLSKSSLVM
                     GK"
BASE COUNT          541 a          818 c          795 g          476 t
ORIGIN      
        1 aacccggccc acacggccaa agttcgcatc ttcacgcctg gcgcggagat tccgtttgcc
       61 ggtcacccaa cagcgggcgc ggccgtgttg cttgccgagc tgcgcgcccc tgaaccgagc
      121 gaggatctcg atgcgatcat cgcactcgaa gaaaacatcg gcattgtgcg cgttggcgtc
      181 aggctgagaa agggagaggc ggcatttgcg gagttcgacg ctcccaagct gcccgaacag
      241 tcgggcacgc ttccacccgt tgagtcgatt gcagcggccg tgagcctgct cccccgcgag
      301 attggcttcg caaatcatcg cccgactaga tttgcggccg gaaacagctt tgcctttatt
      361 ccggttgcat cactggaggc gatggcaaag gctgaagtga acagccagca ctgggcaacc
      421 gctatatccg ggcagggcct cgccggcgcc tttctctaca cccgcgagac ggtccacacg
      481 acatcctcct tccacgcacg aatgttcgcg cccgacttgg gtgtgccgga ggatccagca
      541 acgggctccg cagccgtgac attcgccggc gtgattcaga tgttcgacga gctgcccgat
      601 ggccaccaca agcgcatcat agagcaaggc ttcgaaatgg gccgtccgag catcatctcg
      661 ctcacgctcg aggtggcagg cggtcagctg cgcaacgtcc gcatcggggg cagtgcggtc
      721 cgcgtcacgg aaggccgggt cacgatctga atagtacgga cacaagtaaa atgccgaact
      781 cgaagctcgc tattcctgcc atcgctatct gcggcctgct ccagattcat tcaccgcaac
      841 cggcttcagc tgaacaggtc gtctggttcg gcaaccgaag cgaggatggc gcgtcgctcg
      901 tctatggaac gccaaacagc ggctacggaa aaatcgcgtt cagttgtgag gccggagaag
      961 acgacgtgca ttttgtctac gagcacaagc caataaggcc tgaggaaggc gtgcaggtcg
     1021 aggttttcct ggcggccggt ggcgtcgaga ttgctattcc aacaactgga ggacgccttg
     1081 agattgacga cgcctttctg ctcgaaggcc agacgaagct cgacgacaac ttgcgcaaga
     1141 tcctgaccgc aggcggccag ctgattgtca ccgtagagga cggcgccgag gagtttccgc
     1201 tcgacggcgc agcggaagca gcaaagcatc ttctccaggt ttgctcgccg aacgggtgac
     1261 cacgctcgcg cccctggctt ccttttgcat caaaggaggg ttgcaaagcc ccgacgcatc
     1321 cgctactcac catggggtgc ttaggcgcgt gccaggcact ttggaaccag accagcagac
     1381 tgccgatgcc cacagtgaat agtggccctg ctagagctat cgacgcggcc gtcgccgcgc
     1441 cagcaggtgg cttccgtctg ctcgctctcc ttcgcgatct gctccttact agcccctgag
     1501 cctcgacggt ctcggaacga tccgggacgg acgctcaggg ggaccatgat gaagcaaaac
     1561 ggatcgtgtt aaacctcacc ggccttcact tcctgccggt accgcgaagg aaaattcacc
     1621 aatgtctcag acgacaaact ccggcgatcg agtattgatt tttgacacaa cgctgcgcga
     1681 cggcgagcag tgccctggcg cgaccatgac gttcgaggag aagctcgagg tcgccgacct
     1741 gctcgacgaa atgggcgtcg acatcatcga agccggcttc cccattgctt ccgatggaga
     1801 tttcgaggcg gtttccgcca tcgccaaacg ggtcaagcgg gctgttgtgg ctggccttgc
     1861 acgcgcaagc ttccaggata tcgaccgttg cgccgaagcc gtccggcacg cccagcgtcc
     1921 gcgcattcac acgttcatct ccacctcgcc catccacatg aagtacaagc tccagaagga
     1981 gccgcacgag gtgttggaga tggtgcgggc ctccgtggag cgcgcccgca aatacgtgga
     2041 cgacgtggag tggtcggccg aagacggcac ccggacagaa atcgacttcc tctgccgttg
     2101 cgtcgagacg gccatcaagg ccggggccac caccatcaat attcctgaca ccgtcggcta
     2161 cacggttccc gaggagtact acaacctgtt caagacggtg cgtgagcggg tcccgaattc
     2221 agacaaggcc gtcttttccg tccattgcca tgacgacctc ggcatggccg tggctaattc
     2281 tcttgcgggc gtcaaggcgg gtgcgcggca ggttgaatgc accatcaacg gcatcggaga
     2341 gcgcgcgggc aacgccgcgc tcgaagagat cgtgatggcg atcaagaccc gcaacgatgt
     2401 gctgccgttc tggaccggta tcgaaaccag gatgctgacc cgcgcttcga agctggtttc
     2461 cgcggcgacc tcgttccccg tgcagtacaa caaggcgatc gtgggccgga acgccttcgc
     2521 gcacgagagc ggcatccatc aggacgggat gctgaagcac acccagacct acgagatcat
     2581 gacgccggag tcggtgggcc tcagcaagag ctcgctggtc atgggcaagc
//
LOCUS       DASUMG010000166         2461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_904987, whole genome shotgun sequence.
ACCESSION   DASUMG010000166 DASUMG010000000
VERSION     DASUMG010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904987"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..2461
                     /locus_tag="VFX46_02695"
                     /pseudo
     CDS             66..2461
                     /locus_tag="VFX46_02695"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
BASE COUNT          447 a          821 c          760 g          433 t
ORIGIN      
        1 tcctcacgct cggtcagcgc cgagctgaaa aagggcagtg cacgctattt ctggaagacg
       61 tggcggctca caatgagggt cagatcttca tcgccctgcc gccggaggac tggagttggc
      121 gcgaagcatt gagggaggca cgagaggagc ttaatcccag cggtgctacg attattccgt
      181 ttcgggctcg aactaattcg tcggatggag aggcttcgac ggaagatcga tcccccacag
      241 acccgcctcc ttccgaggga aatgcaaaga gcttcgaaac cgagttgaag cgcatcaaaa
      301 cggcgctcgc accagccccc gtctatcttg ccgcctcgta tcgctaccaa ggcgacgacc
      361 gcgcacgcat tgcggctctt gctcagctga cggagcgcgt gggcacaccc ctcgttgcga
      421 ccaatgacgt gctctaccac gtcccccagc gccgggcact gcaggatgtg ctcacctgca
      481 tccgcgaagg ggtgacgatc cacgaggcgg gcctgaagct cgcgcccaac gccgagcgcc
      541 atctgaaaag tccatccgaa atggcgcgcc tgttcgccgg ttacgaggct gcgctcgccc
      601 gcaccatcga gatcgccgag gcctgccgct tctcgctcga cgagcttgtc tacgaatatc
      661 ccgacgagcc ggtgccgccg ggaaaaacgc cccaatccta tctggaagag ctgacctggg
      721 agggcgcccg ctggcgttat ccggaaggca ttcctgaaaa agtcgcgcag ctcctccggc
      781 gggagctgaa ccttatcgcc cagctcaact acgcgcccta cttcctgacc gtctacgaca
      841 tcgtcaaatt cgcccgttcc caaaacatcc tggcccaagg caggggctcc gcggcgaact
      901 cggccgtgtg ctactgcctc ggcatcacgg cggtgaaccc gaccgagatc gacgtgctgt
      961 tcgagcgctt cgtctcgccc gagcgcaagg agccgccgga tatcgacgtc gatttcgagc
     1021 acgagcggcg cgaggaggtg atccagtaca tctacgcccg ctacggccgc gatcgggccg
     1081 ggcttgccgc gaccgtcatc tcctaccgcg cgcgctccgc cgtacgcgag gtcggtaagg
     1141 ccatggggct gtcggaggat acggtcgcgg cactcgccgg catggtctgg ggtgtctccg
     1201 gtgggacggt gccggaaaaa catgtgcggg aggcggggct cgatcctcgc gatccgctgc
     1261 tggctcaggt cctggcgctc gccgacgagc taatcggctt cccgcggcac ctttcccaac
     1321 acgtcggcgg cttcgtgctc acccgttcgc ctctgtgcga ggtcgtgccc atcggcaatg
     1381 cggcgatgga gaaccgcacg gttatcgaat gggacaagga cgatctcacc gcccttggcc
     1441 tgctcaaggt ggacgtgctt gccctcggca tgctgacctg catccaccgc gccttcgatc
     1501 tcatcgagac gcactacggc aagcgcctga cgctggcgaa catcccgcgc gaggacccaa
     1561 aggtctacga catgctctgc cgcgcggatt ccatcggcgt gttccaggtg gaaagccgcg
     1621 cgcaaatgaa catgctgccg cgccttaagc cgcgcacttt ttacgacctc gtcatcgagg
     1681 tggcgatcgt ccgtcccggg ccgatccagg gcgacatggt gcacccttat ctcaggcgcc
     1741 gggcgggtct tgagcctgag acctatcctt cacctgcccc ggagcatgga cctcctgacg
     1801 agctgcgcca aatcctcagc aagaccaaag gcgtgccgct gttccaggag caggccatgc
     1861 gcattgccat ggtggcggcg aagttctcgg acgcggaggt gaacgagctc cgcaaagcca
     1921 tggccacctt ccgccgcgct ggcaccatcg gcaagctcga gcacaagatg gtgagccgca
     1981 tggtggcccg cgggtacgat cccgagttcg cggcgcgctg cttcagccag atcaagggct
     2041 ttggcgaata cggcttcccg gaaagccacg ccgccagctt cgcccatctc gtttatgttt
     2101 ccgcctggat caaatgccat ttcccggcgg cgtttgcggc cgccctgctc aactcgcagc
     2161 cgatgggctt ctacgcaccg gcccagatcg tccgtgatgc ccgtgagcac ggcgtcgagg
     2221 tgcgccccgt agacatcaac cattcggatt gggactgcac gctggagccc atttcaggga
     2281 gtggcgaaaa caccgttgtc gcgctgcggc ttggcctgcg ccaggtcgat ggtctggctc
     2341 aggaggaagc acagaagatc gttgatgcgc gccaaggccg gcctttccga gacgtccacg
     2401 acctgtgggc ccgcgcaggc gtgcggctgg ccaccatcga gaagctcgcc gcggccgatg
     2461 c
//
LOCUS       DASUMG010000167         6479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_583244, whole genome shotgun sequence.
ACCESSION   DASUMG010000167 DASUMG010000000
VERSION     DASUMG010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6479
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583244"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1386)
                     /locus_tag="VFX46_02700"
     CDS             complement(229..1386)
                     /locus_tag="VFX46_02700"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599522.1"
                     /translation="MRRADLIALAQSTGFPRISIYLPTHLTYPEIEQDPIRLSNLLKQ
                     ADEQLEAAGVRRPEIDELLEEARGRVPASMFWRYQEHGLAVLIEKGKTHWVKLPRSVP
                     ELVVLSPRYHLRPMIPMFRNGENFNLLAVSRENIRFYRGRERELVEVAVENMPSGMTE
                     IRQRTEYDDQLGFHARDRGDPASGMDHGKFHTLGVSPEDYENVLFDTLLREVAKAVDR
                     HLASSEEPLVLAAEPRTLGHLRKHLAYRHIAQDTIQSDPVGLSKEELHSEAWRIAQPI
                     IRADRENARRRLRAWLDGASIPGAKDPQMLLRDAIAGRVECVFLAQNANVWGYFDEEF
                     QVLRIDPEPGPGNEDVLNRLAIETLIRSGDVFTMPEDLEGRIGPVAGLYRY"
     gene            1754..2386
                     /locus_tag="VFX46_02705"
     CDS             1754..2386
                     /locus_tag="VFX46_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5599523.1"
                     /translation="MRHWAAALAGLVVAIPLVAADPLDGHDCFSSDNDRRIKGCTELI
                     ERPELTGQLLGSAYAMRALGYSLKGLYDEAIRDYDEAIRLVPDFAVALNNRAWAYFKS
                     GRAEEGLPDVERSLQLQPTSPHAYDTRAHIRQVMGDPEGALRDYEAAMRFGGERMVQL
                     YQCGLAALGLYGGPVDGIYTPILRRAIEACVTSKTCDPLPPDEECRAVTS"
     gene            2471..3019
                     /locus_tag="VFX46_02710"
     CDS             2471..3019
                     /locus_tag="VFX46_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5599524.1"
                     /translation="MKFVASFAAALLLPLLGMTASAQESSSGITISQPWARATPGGVK
                     NGAAYMQITASAADKLVDARSDAAERVELHNHIHENGVMKMRRVESIEVPAGETVTLG
                     PGGYHVMLMGLKQPLKAGEELELTLVFEKAGEINVTASIEPIGARGPGGAAHADHGHD
                     HNHHHDHDHDHDHGEHHHEHKH"
     gene            complement(3078..3989)
                     /gene="speE"
                     /locus_tag="VFX46_02715"
     CDS             complement(3078..3989)
                     /gene="speE"
                     /locus_tag="VFX46_02715"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX5599525.1"
                     /translation="MKRWIEETFHPHWRVGMEADKILHEEKTEHQHLVIFENAVWGTV
                     LILDGVFQLTTLDEFIYHEMMAHVPLMALERPRRVLVIGGGDGGVLREVLKHPSVEKA
                     TLCEIDRSVIDLSLKYYPQVSNGAFDNPRADIVIGDGLKYVAETTEKFDAIIVDSSEP
                     IGPSAVLHTEEFFANCKRALKSGGIVVTQNGLPFLCADAFASTTRILSSLFKCCAPYL
                     CHQPCYFGGEFAINWGSDEPAHLEIPAAKLARRQKARKIETRYWTPDFHVAAFALPAY
                     AQKTVNEAKAAAAGRGGKAKQKSKAAT"
     gene            complement(4117..4692)
                     /gene="speD"
                     /locus_tag="VFX46_02720"
     CDS             complement(4117..4692)
                     /gene="speD"
                     /locus_tag="VFX46_02720"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HEX5599526.1"
                     /translation="MAYDDTLFQLGMDLTRSSTAQKEDFGNAPGAQNGEQVRGSHEDR
                     KDFFIERDGVRFAGSHLIIDLFGAKRLDDLRHIERTLKRCVEIAGATLLHIHLHHFTP
                     NGGVSGVAVLSESHISIHSWPEADYAALDVFMCGDAKPHLTIDVLKHAFDARHVVVKE
                     HRRGEELTELSWQAAADRKAPDRGEKVRRAA"
     gene            4987..5781
                     /locus_tag="VFX46_02725"
     CDS             4987..5781
                     /locus_tag="VFX46_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX5599527.1"
                     /translation="MHPEYETLRLDEPVPHVTVITMNRPQVLNALNTQMMAELRDCFA
                     SYYVDQGETRCLVLTGSGEKGFCAGADLKERNGMSDEVWRRQHAIIEQAIRAIMDCPI
                     PIIAAVNGAAYAGGCELALACDFIYAVPHARFAQTEVALGIIPGAMGTQNLPRSIGIR
                     RAKEVILTATPFTAEEALAWGLVNKLVPSDRLMAEVLDTASRIAANAPIAVRQAKRAL
                     DKSQDLDRANGYSFEIEAYNRTVDTEDRHEGIRAFNEKRKPVFKGR"
     gene            5814..>6479
                     /locus_tag="VFX46_02730"
     CDS             5814..>6479
                     /locus_tag="VFX46_02730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5599528.1"
                     /translation="MTTDRAEAVTISVDPEWQELRDGVRRICDDFPNEYWVKLDHESA
                     YPTEFVNALTEAGYLAALIPEEYGGSGLPLSAACAILETIHEQGCNAAACHAQMYTMG
                     TLLRYGSEAQKSKYLPGIASGELRLQAFGVTEPTTGSDTTQLKTRAEKKGDRYIVTGQ
                     KVWTSRAMQSDLMLLLARTTPADQVRKRSEGLSVFIVDLREAKGRGLELRKIDAMINH
                     NTCE"
BASE COUNT         1285 a         2068 c         1895 g         1231 t
ORIGIN      
        1 acgaaacttt cgaggacctg gacgtagggg atccagtcga gctgacgatc gcctccgacg
       61 aaagcccacg gggtccgcag gccaccagca tccgtcgcat ccggccattg cgttacaacc
      121 cgcagccaac ctgatggccg cgatttagcg aaccttcaga aatattgcgg ccaccgcctt
      181 gggcggtggc cgatgctttt tcttgaattt ctgtaacggt aggcgcgctt aatacctgta
      241 gagccccgcg accggcccta ttctgccttc aagatcctct ggcatggtga agacgtcacc
      301 gctgcgaatt agcgtctcaa tggccaagcg attgagcacg tcctcgttgc ccggacccgg
      361 ctcaggatcg atgcgaagca cttggaactc ctcatcgaaa taaccccaaa cattggcgtt
      421 ctgagctagg aacacacatt cgactcgccc ggctatcgca tcccgaagca acatctgtgg
      481 atccttggcg cccggaatgg aagcgccatc gagccaagcc cgcagacgcc ggcgcgcatt
      541 ctcacgatcg gcacggatga tcggctgggc gatgcgccac gcttcggaat gaagctcttc
      601 cttgctcagc ccaactgggt ccgattgaat cgtatcctgc gcgatgtgcc tgtaggcgag
      661 atgcttgcga agatgcccca aggtgcgcgg ctctgctgcg agaacaagcg gctcttcgct
      721 gcttgccaaa tgacgatcaa cagctttggc cacctcccga agcaaggtgt cgaacagaac
      781 attttcataa tcttcgggac tcacacccag cgtatggaat ttgccgtggt ccattccgct
      841 cgctggatcg ccacggtcgc gggcatgaaa tccaagttga tcgtcatact ccgtgcgctg
      901 ccggatctcg gtcataccgc tcggcatgtt ctcgaccgcg acctcgacca gctcacgctc
      961 acgtccccga tagaaccgaa tattttcgcg cgatacggcg agcagattga aattctcgcc
     1021 attccggaac atcggaatca tcggacgtag atgataccgt gggctgagca ctacgagctc
     1081 cggaacggac cggggcagct ttacccaatg cgtttttccc ttctcgataa gcacggcgag
     1141 accgtgctcc tgatagcgcc agaacatgga tgcgggcaca cgcccccgcg cttcttcaag
     1201 gagctcatcg atctccggtc ggcgaacccc cgctgcttcc aactgctcgt cggcctgctt
     1261 gagcaggttc gacagtctta tcggatcttg ttcgatttcc gggtaggtca ggtgcgtcgg
     1321 tagatagatg ctaattcttg gaaaaccggt cgactgcgca agcgcaatga ggtcggctcg
     1381 tctcatagct ttcccacttt ttgtcagctg gcctcaaaga gcgtaagcga agacgccagc
     1441 ttttctgttc gtttactccg gcccgttggg aaaaactcgc ctccggtccg ccaagttccg
     1501 ccaccggagt cacggcactc ccacaagcct tgaccccagc ctacccacag cgcggccccc
     1561 cagccttgac ttttaccaat atcgagcagg aattaggtgt gtgcaattgg cgagagctgc
     1621 ctgccacatt ggcccgaggg ccgtatcacg aatgctgtga cgaggctcgt ttcggcaacg
     1681 ccattgaatc gtcgggttcc agtcggaaac cgtcggaaaa tggatggtac agctccatga
     1741 agggatgcag gtcatgagac attgggcagc ggcactcgcc ggtctcgtcg tggcaatccc
     1801 ccttgtcgca gcggacccct tggatggcca cgattgcttc tcctccgaca atgaccggcg
     1861 catcaagggg tgcacggagc tgatcgagcg cccggagctc acagggcagc tgctgggatc
     1921 ggcttacgcc atgcgggcgc tcggctactc gctcaaaggc ctctacgacg aggccatccg
     1981 cgactacgac gaggcgatcc ggctggtgcc ggacttcgcc gtggccctca acaaccgggc
     2041 atgggcctat ttcaagtcgg gtcgggcgga ggaaggcctg cccgacgtcg aacgttcgct
     2101 ccagctgcag cctacgagtc ctcatgctta tgacacgcgg gcgcacatcc ggcaggtgat
     2161 gggcgatccg gaaggagcac tgcgcgacta cgaggcggcc atgcgattcg gcggcgagcg
     2221 catggtccag ctctaccaat gcggacttgc ggcattgggt ctctatggcg gccccgtcga
     2281 cggcatctac actccgattc tgcggcgcgc catcgaggca tgcgtgacca gcaagacctg
     2341 cgatcccctc ccgccggacg aggaatgccg cgccgtcaca tcgtgacgga tctggtcgag
     2401 tccgcttgac ccctcggccc gatccaactg cgagccaacc attccggtat cgagttgagg
     2461 agacacagct atgaagtttg ttgcgagctt tgctgcggcc ctattgctgc ccctgctggg
     2521 catgactgcc tctgcccagg aaagctccag tggaatcacc atttcccagc cctgggcgcg
     2581 agcaacccct ggcggcgtga agaacggcgc ggcttacatg cagatcacag cctcggcggc
     2641 cgacaagctc gtggacgccc gatcggatgc agccgaacgc gtcgagttgc acaatcatat
     2701 tcacgagaac ggcgtgatga agatgcgccg cgtcgaatcg atcgaggtgc cagccggcga
     2761 gacggtgacg ctcggccccg gcggctacca cgtcatgctc atgggcctga agcagccgct
     2821 caaggccggt gaagagctgg agctgacgct tgtctttgaa aaggccggcg agattaacgt
     2881 gactgcctcg atcgagccga ttggtgccag aggcccagga ggggctgcac acgcggacca
     2941 tggtcatgat cacaaccacc atcacgatca cgaccatgac catgatcacg gcgagcatca
     3001 ccacgagcac aaacactagc cgcgcgctga tgctctcgcg tcacccaata cctgcaacga
     3061 acggcggcgg cccggctcta ggtcgccgcc tttgatttct gctttgcctt gccccctcga
     3121 ccagccgccg cagctttcgc ctcgttcacg gtcttctgcg cgtatgcggg cagagcgaac
     3181 gctgcgacgt ggaagtcggg cgtccagtag cgggtctcaa tcttccgagc cttctgccgg
     3241 cgggcgagct tggccgccgg tatctccaaa tgtgccggct catccgatcc ccaattgatg
     3301 gcaaactcgc cgccgaagta gcagggttgg tggcacaagt acggtgcgca acatttgaac
     3361 agcgacgaga gaatacgcgt cgtgctggcg aaggcgtccg cgcagaggaa cggcaggccg
     3421 ttctgggtta ccacaatgcc gccagacttc agcgcccgct tacagttcgc gaagaactcc
     3481 tccgtgtgca gcaccgcgga cgggccgatt ggctccgagc tgtcgacgat gatcgcatcg
     3541 aacttctcgg tcgtctccgc cacatacttc aggccatcac cgatcacgat gtcggcccgc
     3601 gggttgtcga aggcgccatt cgagacctgc ggataatact tcagcgagag atcgatgacg
     3661 ctgcggtcga tctcgcagag cgtcgccttc tcgacggacg gatgcttcaa gacctcacga
     3721 agcacgccgc cgtcgccgcc gccaatgacg agcacccgcc gcggtctctc gagggccatc
     3781 agcggcacgt gggccatcat ctcatggtag atgaactcgt cgagcgtggt gagctgaaag
     3841 acgccgtcga gaatgagcac cgtgccccag acagcattct cgaaaatgac aaggtgctga
     3901 tgctcagttt tctcctcatg gaggatcttg tccgcctcca tgccaacacg ccagtgggga
     3961 tgaaacgtct cctcgatcca gcgcttcatc gaccagcctc catcgttgtg caggcctctc
     4021 cctgccttaa cccgtccaat ccgaacagcg aatgaggcgc gcacggtcca gagagacccg
     4081 cacgcgcccc attcttgatt cgttggctcc gcaggctcag gccgcccgcc ggaccttctc
     4141 cccgcgatcc ggagccttgc ggtcggcggc agcctgccag ctcagctccg tcagctcctc
     4201 gccgcgcctg tgctccttca cgacgacgtg gcgtgcgtca aaagcatgct tgagcacgtc
     4261 gatcgtaagg tgaggcttgg cgtcgccaca catgaacaca tcgagcgccg cgtagtcggc
     4321 ctcgggccag ctgtgaatgc tgatgtggct ctccgaaagg acagccacgc ccgagacgcc
     4381 cccgttcggc gtgaagtggt ggaggtggat gtgcaggagc gttgctccag ctatctccac
     4441 acaccgcttc agtgtccgct cgatgtggcg cagatcgtcg agtctcttcg cgccgaagag
     4501 gtcgatgatc aggtggctac cggcgaagcg gacgccatcg cgttcaatga agaagtcctt
     4561 cctgtcctcg tgcgacccgc gcacttgttc gccattttgc gcaccgggcg cgtttccaaa
     4621 gtcttccttt tgggcggtgc ttgaacgagt caagtccatc cccaattgga agagggtgtc
     4681 gtcgtaggcc atgtgaccct ccccaaccag cagacagagg tgacccgccc gctccttacc
     4741 ctttggggtt ccagggaact cctttgacct ttggggcagg gggacgaacg gagagcaccc
     4801 atatgcgatc aatgggcttt ctaatcaagt gcaaaattgc cttacccggc aaatttttgt
     4861 cggccgcgtt tacgagccgc tacgacgttt gatttgcttg tcgtgcaagg cctttttctg
     4921 cgtcgcagct agccctcagc ctcgctttcc cgtatctaaa atcggtcacg aaactgggag
     4981 ttcaacatgc atcccgagta cgagactctg cgcctcgacg agcccgttcc tcacgtcacc
     5041 gtcatcacga tgaatcggcc gcaggtcctc aatgcgttga acacgcaaat gatggccgag
     5101 ctacgtgact gcttcgcctc ctactacgtc gatcagggcg agacgcgctg cctcgtgctc
     5161 acgggatctg gcgagaaagg cttttgtgcg ggcgccgacc tcaaggagcg caacggcatg
     5221 tcggacgagg tctggcggcg ccagcatgcc atcatcgagc aggcgattcg cgccatcatg
     5281 gattgcccga tccccatcat cgccgccgtg aacggcgcag cttacgctgg cggctgcgag
     5341 ctggccctcg cctgcgactt catctacgcc gtgccgcacg cccggttcgc ccagacggag
     5401 gtggcgctcg gcatcatccc aggggctatg ggcacgcaga acctgccccg cagcatcggc
     5461 atccggcgcg cgaaggaagt gattctgacc gccacgccgt ttacggcaga ggaggccctc
     5521 gcttggggcc tcgtgaacaa gctggtgcca agcgaccgcc tgatggccga agtgcttgat
     5581 actgcaagcc gcatcgcggc caatgcgccc atcgccgtcc gccaggccaa gcgagccctg
     5641 gataagtccc aggatctcga ccgggccaat ggctacagct tcgagatcga ggcctacaat
     5701 cggacggtcg acacggagga ccgtcacgaa ggcatccggg cgttcaacga gaagcggaag
     5761 cccgtgttca agggacgctg agtgctgcaa ccaaagcaac gagccagtcg aagatgacca
     5821 ccgatcgcgc cgaagctgtc acaatctccg tggatcccga atggcaggag ctgcgcgacg
     5881 gtgtccggcg aatctgtgac gactttccca acgagtactg ggtgaagctc gaccacgagt
     5941 cggcctatcc aaccgaattc gtgaatgcct tgactgaggc cggttatctg gccgcgctca
     6001 tcccggagga atacggcgga tccgggctgc cgctgagcgc tgcctgcgcc atcctcgaaa
     6061 ccattcacga acagggctgc aacgcagccg cctgtcatgc ccagatgtac accatgggca
     6121 ctctgctgcg gtacggctcg gaggcgcaga agagcaagta cctgccgggg atcgcctcag
     6181 gcgaattgcg cctccaggcc tttggtgtga cggagccgac caccggcagc gacaccaccc
     6241 agctcaagac ccgcgccgag aagaagggcg atcgctacat cgtcacgggc cagaaggtct
     6301 ggacaagccg cgccatgcag tcggacctca tgctgctcct ggcgcggacc acacccgccg
     6361 accaggttcg gaagcgttcc gaaggactct ccgtcttcat tgtcgatttg cgggaggcca
     6421 aaggccgggg cctggagctt cggaaaatcg acgcgatgat caaccacaac acttgtgag
//
LOCUS       DASUMG010000168         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_503644, whole genome shotgun sequence.
ACCESSION   DASUMG010000168 DASUMG010000000
VERSION     DASUMG010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503644"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1449
                     /gene="glyA"
                     /locus_tag="VFX46_02735"
     CDS             145..1449
                     /gene="glyA"
                     /locus_tag="VFX46_02735"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX5599529.1"
                     /translation="MTSALSAQARASSGFFTEALAERDPELFGAVAAELSRQQNEIEL
                     IASENIVSRAVLEAAGSVLTNKYAEGYPGRRYYGGCQHVDVAEQLAIDRAKKLFGCEF
                     ANVQPHSGSQANQGVFMALAQPGDTILGLSLAAGGHLTHGSPVNMSGKWFKAVHYGVR
                     RQDHRIDHDEVRALAKEHRPRIIIAGGSAYPRTIDFAAFREIADEVGAYLMVDMAHFA
                     GLVAGGVHPNPVPYAHVVTTTTHKTLRGPRGGMILTNDPEIAKKVNSAIFPGLQGGPL
                     MHIIAAKAVALGEALRPEFKAYARAVVENAAALAETLQAAGYDLVAGGTDTHLLLVDL
                     RSKGLTGNVSERALGRAHITCNKNGVPFDPEKPTVTSGIRLGSPAGTTRGFGVEEFKA
                     IGRMIAEVLDGLAKNGEEGNAAVEARVREEAIALCRKFPIYT"
     gene            1592..2086
                     /gene="nrdR"
                     /locus_tag="VFX46_02740"
     CDS             1592..2086
                     /gene="nrdR"
                     /locus_tag="VFX46_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX5599530.1"
                     /translation="MRCPFCGGSDTQVKDSRPTEENAAIRRRRVCPDCGGRFTTFERV
                     QLREITVIKRSGRKVPFDRDKLLRSVEIALRKRPVEQERIERMVSGIVRQLESSGESE
                     IPSSTIGEIVCEALRGLDPVAYVRFASVYRDFREVSDFHEVLTEIAKENGTGQKAHGP
                     HDIE"
     gene            2043..>3016
                     /gene="ribD"
                     /locus_tag="VFX46_02745"
     CDS             2043..>3016
                     /gene="ribD"
                     /locus_tag="VFX46_02745"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX5599531.1"
                     /translation="MAPARKRMARTTSSDCQSARDARFMDIALMLARRGLGTTAPNPA
                     VGAVIVDETTGEVIARGWTQPGGRPHAETEALRRAGARARGATMYVTLEPCSHHGGTP
                     PCADALVEAGIARVVVGIEDPDPRVAGRGLDRLRAAGISVTRGVRSAEADWVTRGHIL
                     RVSERRPFIQLKLALAADGSVPRGKAGQPVWVTGPEARAHGHLLRATADAILVGRRTV
                     EDDDPELTCRLPGLAGQSPARVVLTRDLRISLESKLVRTARDVPVILFCGPDADSQQR
                     AELEARGCEIIPVREVEGTLWLPAVTEALAARNVTRLLVEGGPAIWRS"
BASE COUNT          500 a          965 c         1032 g          519 t
ORIGIN      
        1 cttctgcggg gaaagcccgc gccatccggt cccaacccga ccgcacatgg ttgttgtagg
       61 aggctggccc gtgctatgcg ctcgcgaccg gggccgatgc gctgacccta tttctgcgac
      121 tagttcataa cgagcgaaga cgagatgacc tccgcccttt ccgcgcaagc gcgcgcgtcc
      181 tcaggctttt tcaccgaggc gcttgccgaa cgagatcccg agctgttcgg cgccgtcgcc
      241 gctgagctct cgcggcagca gaacgagatc gagctgatcg cctcggagaa catcgtttcg
      301 cgggccgtgc tcgaggctgc ggggtcggtg ctcacgaaca aatatgccga aggctatccg
      361 gggcgccgct attacggcgg gtgtcagcat gtcgacgtcg ccgagcagct ggcgatcgac
      421 cgggccaaga agctcttcgg ctgcgagttc gccaatgtgc agccgcattc cggcagccag
      481 gccaatcagg gcgtgttcat ggccttggcg cagccgggtg acaccattct cgggctttcg
      541 ctcgcggcgg gcgggcatct gacgcacggt tcgccggtca acatgtccgg caagtggttc
      601 aaggccgttc attacggcgt gcgccggcag gaccatcgca tcgatcatga cgaggtgcgt
      661 gccctggcca aggagcatag gccgcgcatc atcatcgccg ggggatcggc ctatccgcgc
      721 accatcgatt tcgccgcctt ccgcgagatt gccgacgagg tcggggctta tctgatggtg
      781 gacatggctc attttgccgg tttggtcgcg ggaggcgtgc atccgaatcc cgttccctat
      841 gcgcacgtgg tcacgacgac cacgcacaag acgttgcgcg gcccccgcgg gggcatgatc
      901 ctgaccaacg accccgaaat cgcaaagaag gtcaactcag ccattttccc gggcctgcag
      961 ggcggcccgc tgatgcacat catcgcggcc aaggcggttg ccttgggcga agcgctgagg
     1021 ccggagttca aggcgtatgc ccgggccgtc gtcgaaaatg ctgccgccct cgctgagacg
     1081 ctgcaggcag ctggctacga tctggtggcc ggcggcaccg acactcactt gcttctggtc
     1141 gatctcaggt cgaaggggtt gaccggaaac gtctccgagc gggccctcgg ccgagcccac
     1201 atcacctgca acaagaacgg agtccccttc gatcccgaga agccgaccgt gacctcaggg
     1261 atccggctcg gctcccctgc cggcacgacg cgcggcttcg gtgttgagga gttcaaggcc
     1321 atcggccgca tgatcgcgga ggtgctggat gggctcgcca agaatggcga ggagggtaac
     1381 gccgccgtcg aggcgcgtgt gcgtgaggag gcgatcgccc tctgccggaa gtttccgatt
     1441 tacacctagg cccgtctccc gcgctggtgt gtgggctccc gaagggcacg gaacagggag
     1501 aataggatat cggtggcaag cgccgggatg accttaacgc gatgatccgg caacggcata
     1561 ctcagccgtc cggatgaggg gagatcacgc catgcgttgc cccttctgcg gcggcagcga
     1621 tacgcaggtg aaggacagcc ggccgacgga ggagaatgcg gccatccgac ggcgccgggt
     1681 gtgcccggat tgcggcggca ggttcacgac cttcgagcgc gtgcagctgc gagagatcac
     1741 cgtcataaag cggtcgggcc gcaaggttcc cttcgatcgc gacaagctgc ttcgatccgt
     1801 cgagatcgcc ctccgcaagc ggcccgtcga gcaggagcgg atcgaacgca tggtgtccgg
     1861 catcgtacgg cagctcgaga gctcggggga atcggagata ccctcctcca cgatcggtga
     1921 gattgtgtgc gaggccctgc gcgggctgga cccggttgcc tatgtaagat ttgcgtccgt
     1981 ttaccgtgat ttccgcgaag tctcggattt ccacgaggtg ctgaccgaga tcgcgaagga
     2041 gaatggcacc ggccagaaag cgcatggccc gcacgacatc gagtgactgt cagtccgccc
     2101 gcgacgcccg tttcatggac atcgccctga tgcttgcccg ccgagggctc gggaccacgg
     2161 cgccgaaccc tgccgtcggc gctgtgatcg tcgacgagac cacgggcgag gtgatcgcgc
     2221 gcggctggac gcagcccggc ggccgtccgc acgcggagac cgaggcgctg cggcgcgccg
     2281 gagcccgtgc tcgcggggcg accatgtacg tgacgttgga accctgctcg caccacggcg
     2341 gcacgccgcc ttgcgccgat gctctcgtgg aggcaggcat cgctcgcgtt gtggtgggga
     2401 tcgaggaccc cgatccacga gtggccggtc gcggcctcga tcgcctacgc gctgccggca
     2461 tttccgtcac ccgcggcgtg cgcagtgcgg aagcggactg ggtgacccgc gggcacattc
     2521 tgcgcgtgag cgaacgccgg ccgttcattc agctcaaact tgcgctggct gccgatggca
     2581 gcgttccgag aggcaaagcg gggcagcctg tctgggtgac aggacctgag gcccgcgcgc
     2641 atgggcatct gttgcgtgcc acggccgatg ccattctcgt cggtcgtcgg acggttgagg
     2701 atgacgatcc ggagctgaca tgccgcctgc ccgggcttgc cggccaatcg cccgcccgcg
     2761 tcgtgctgac acgcgacctg cggatcagcc tcgagtccaa gctcgtccgt acagcccgtg
     2821 acgtgccggt gatcttgttc tgcgggccgg atgccgactc ccagcagcgt gcggagctgg
     2881 aggcacgcgg ctgcgagatc atccccgttc gcgaggtcga ggggaccctt tggctgccag
     2941 ctgtcacgga agctctggcg gccaggaacg tcacgcgctt gcttgtggaa ggcgggccgg
     3001 cgatatggcg ctcctt
//
LOCUS       DASUMG010000169         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_985685, whole genome shotgun sequence.
ACCESSION   DASUMG010000169 DASUMG010000000
VERSION     DASUMG010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985685"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..445
                     /locus_tag="VFX46_02750"
     CDS             149..445
                     /locus_tag="VFX46_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599532.1"
                     /translation="MDDLRNDLTQRLDELEAHIGEMRKTLEHHGMISDQLQSEWDDML
                     RQHAELRRRLRSGEAKGSEAGATLGQDIDVLRHAFFRWAARVDKRFSAGPNDRS"
     gene            870..1271
                     /locus_tag="VFX46_02755"
     CDS             870..1271
                     /locus_tag="VFX46_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599533.1"
                     /translation="MRKAFVATLMCGVMAVQPVSATVLTPAPEAVRPATSSIVPAVKL
                     STDAETAKVIEVGRRHYPRYKRYRRKSRGGAFGAGLAVGIMTALIAKGISESHARNRF
                     DRCARDFRSFDYETGTIITRSGREVLCPYLR"
     gene            complement(1348..2247)
                     /locus_tag="VFX46_02760"
     CDS             complement(1348..2247)
                     /locus_tag="VFX46_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007606498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5599534.1"
                     /translation="MQKNAGLELLTTAEAAEYLRLKERKLYELIAERQIPCTKVTGKW
                     LFPRADLDRWLLAGMVRPEGVVPADPPPIVGGSHDPLLQWALSESRAGLAIMPEGSEA
                     GYRRFLRGEVIAAAIHFHDLDDPEKDANIEIVSREPTLYDAVLIGFATREQGLLVAPG
                     NPLGLKSIRDVLDRKARLIVRPAGAGAQQLLLALLRREGAELQDVAATMEAPTGPDIA
                     QAIRAGHGDCGVATRSVATAAGLDFVPLQIERFDLLMRQRDAFRPALQKLLKLLNSPG
                     FAQRAAELGGLDVSDAGSVRWAP"
     gene            2332..>2691
                     /locus_tag="VFX46_02765"
     CDS             2332..>2691
                     /locus_tag="VFX46_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5599535.1"
                     /translation="MDTSSALELILTGDATLFAIVRLSLLVTLSATFFAALVGLPLGA
                     IVALTNFPGRHAVIVVLNGLMGLPPVVVGLLVFLLLSRSGPLGPLGLLFTPTAMVIAQ
                     TVLITPIIAALSRQVIED"
BASE COUNT          503 a          806 c          821 g          561 t
ORIGIN      
        1 cgatcgtggc tggatgcgca agctcctccg gtcgggacgg tttcagcatg agatgaactc
       61 ttgctcgtac cggtgaagcc atcgaggggg cctgggcgtt gttcgtacgt ggcggggtgc
      121 cacttgcgat cagttgggag caaattcgat ggacgacttg agaaacgacc tgacccaacg
      181 actcgatgag ttggaagcgc acatcggcga gatgcgcaag accttggagc atcacggcat
      241 gattagcgac caactccaga gcgagtggga cgacatgctg cgccaacacg cggaacttcg
      301 tcggcggctg cggtccggcg aggcgaaggg cagtgaggcc ggcgccacct tgggccagga
      361 tatcgacgtc cttcgccacg ccttcttccg ctgggcggcg cgtgtggata agcgcttcag
      421 cgcgggaccg aatgacaggt cataaacgag ccgattggcg gcagaactcg tccgagcccg
      481 cgctttggct ctgggctaaa cggcgctcga ccgccttgtc agtgggccgc gccggagccg
      541 atcacctcgg ttgcgatgat cactgatcag ccaaaatcag ccgcattctt gaagtcaaaa
      601 tttgaagttt gctgacagcc tgacgagctg ctccagtgct gcgaccgagg atcgcccaat
      661 gtcggaatgg tactgatgtt tgcaattcca aagcacttcc taagtgaact ggaaacgctt
      721 gatgtgatag cccaggttca tctgggggat ttggcttgaa tagctgggcg ttttacgggg
      781 gcattcacga tattgccatt ccagtgccgg attacgtcac taggagcatg atgagccctc
      841 tgctcccagc gatcgaagga agtgtgtcga tgagaaaagc ttttgtggca acactgatgt
      901 gcggtgtcat ggccgtgcag ccagtatcag caactgtgct gacgcctgca ccggaggctg
      961 tcaggccggc gacaagttca atcgtcccgg cggtcaagct ttcgactgat gcagagaccg
     1021 ccaaggtgat cgaggtcgga agaagacatt accctcgtta caagcgttat cgccgtaaga
     1081 gccgtggagg tgctttcggc gccggcttgg ctgtcggcat catgaccgct ctcattgcaa
     1141 agggcatcag cgagtcccat gcccgtaatc gtttcgaccg gtgcgcgagg gacttccggt
     1201 ccttcgacta cgagacgggg acgatcatca cccgttcggg ccgtgaggtc ctctgcccat
     1261 atcttcgctg aacaagccgg ttgggcgtta agcagccgcg gcgaaatcat ccgccgcggc
     1321 tattttcgtt ggtgatctcc gctccgatca gggagcccag cgcacgctgc cagcatcgga
     1381 cacatccagc ccaccaagct cggctgcgcg ctgcgcgaag ccaggtgagt tgagcagctt
     1441 cagcagcttc tgcagcgctg ggcggaaggc atcgcgctgg cgcatgagaa gatcgaaccg
     1501 ctcgatctgg aggggcacga agtcgagccc ggcagcggtg gcaacagacc gtgttgcgac
     1561 gccgcaatcg ccatggccgg cccggatggc ctgcgcgatg tcaggccccg tcggcgcttc
     1621 catcgtagcc gcaacatcct gaagctccgc gccctcgcgt ctgagaagag ccagcagaag
     1681 ctgttgggcg cccgcacctg caggccgcac gatgaggcgt gcctttctgt cgagcacgtc
     1741 ccggatcgac ttcagcccca gtggatttcc aggtgcgacc agcaaccctt gctcccgcgt
     1801 ggcaaagccg atcagcacgg catcgtagag cgtcggctcc cgagacacga tttcgatgtt
     1861 tgcatctttt tcgggatcgt cgagatcgtg gaaatggatc gctgcagcga tgacttcccc
     1921 gcgcaggaag cggcgataac cagcttcgct tccctccggc atgatggcca gcccagcgcg
     1981 gctttcgctt agcgcccatt gcaggagggg gtcgtggctt ccgccgacaa tcggcggcgg
     2041 atcggccgga actacgcctt ccgggcgcac catccccgca agcaaccagc ggtcgagatc
     2101 ggcgcgcggg aacagccatt tgccggtcac cttcgtgcac gggatctgtc gctccgcaat
     2161 cagttcgtaa agcttccgct ctttcagtcg aagatactcg gcagcttctg ccgtcgtcag
     2221 caactctaag cctgcgttct tctgcatctt attgcatgga ttctaaaatt tgacatttcg
     2281 ggatcaaaca cactcaatgc ccttggtcaa cgggcattga ggagaggcgc gttggatacc
     2341 tccagcgctc tggagttgat cttgacaggc gatgcgacgc tgttcgccat cgtccgtttg
     2401 tctctgctcg tcactctttc ggccaccttc ttcgcagccc tcgttgggct gccgctcggt
     2461 gcaattgtag cgctcaccaa cttcccgggc cggcatgccg tcattgtggt gctcaacggg
     2521 ctgatgggtc tgccgccggt cgtggtcggc ctgctcgtct tccttctgct ctcacggtcg
     2581 gggccactcg gacccctggg gctgcttttc acgcctacgg cgatggtgat tgctcagacg
     2641 gtgctcatca cacccatcat cgctgccctg agccgtcagg ttatcgagga t
//
LOCUS       DASUMG010000170         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_583771, whole genome shotgun sequence.
ACCESSION   DASUMG010000170 DASUMG010000000
VERSION     DASUMG010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583771"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFX46_02770"
     CDS             complement(<1..238)
                     /locus_tag="VFX46_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HEX5599536.1"
                     /translation="MTTPAETSAAEVARIPLSPDFGWGWFAALIGTGVLVAVLVYTLG
                     VLVFVGVGIWGTNIPFVWGFDLANYVWWIGMANGC"
     gene            complement(222..>2566)
                     /locus_tag="VFX46_02775"
     CDS             complement(222..>2566)
                     /locus_tag="VFX46_02775"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5599537.1"
                     /translation="DRLLAHVQHVFPEARWYVYSPLASETVRDAVADVFGRPVDPVFD
                     LTQADVILTFGGDLLGQGPAHVRYAADYAQRRRTPDRPLPRLYSVETTPSLTGARADR
                     RLAVPPAQAEIIARQVFSRVRQRERSPPQSKTFVDFIAGEIARAGPRSLIFSGDEQPS
                     VVQAIAHALNAELGAVGRTVRYIDPLTPDAAYAQPLMSLAEAIVEGQVEALLILGCNP
                     AYDAPGDLEFAELIGRVPLSVHLGYWLDETSRASLWHIPARHALESWGDLRAFDGTVG
                     LCQPATVPLIEAMSAEEVLARIVGITEPDFVLVQATWREAWNESFESRWRSALEAGIV
                     ANSASPSVPVAAVPNWSPRQPQIAQSSGNPTVVFAPDPSVWDGRFANNGWLQELPKPL
                     TKQVWGNAALMAPAAAAAYRLRTGDIAVFSKGELSIEAPVWLLPGHAPNAVTLPLGYG
                     RRNAGMIGDLNGFDAYRLRSSKHPWILKDVIVEATGRSKELITTQHHHRMEGRNIVQV
                     VAPGEAAKATKPAGASLYPSYIYDGNAWGMTIDLDLCLGCNACVIACQAENNVPVVGP
                     EEVSRGREMHWLRIDRYYRGGSDEPETFFQPVLCMHCEKAPCEIVCPVNATVHSSEGL
                     NQMVYSRCVGTRTCSNNCPYKVRRFNWFNYHQRAPKTPDEIHNPDVTARWRGVIEKCT
                     YCVQWISAARIAARMDKRPIRDGDLVTACQQACPTRAIVFGDINDPSSEVRRRKEDPR
                     NYALLGELNTEPRTTYLARISDPVAARRNSGTPFFNDDTG"
BASE COUNT          546 a          766 c          766 g          488 t
ORIGIN      
        1 tgcagccgtt agccattccg atccaccaaa cgtaattggc gagatcgaac ccccaaacga
       61 acggaatgtt cgtcccccaa ataccgacac ctacgaagac gagcacgcca agggtgtaaa
      121 cgagcaccgc aaccaaaacg cccgttccaa taagcgcggc gaaccagccc caaccaaaat
      181 cgggagacag aggtatgcgg gcgacttccg ctgcggatgt ctcagccggt gtcgtcattg
      241 aagaatggag tgcctgagtt ccggcgagcg gctacgggat cggaaatacg ggcgaggtag
      301 gtcgtgcgtg gctcggtgtt gagctcgccg agcaatgcgt aattccgcgg gtcctccttg
      361 cgccggcgaa cctcgctgct cggatcgttg atgtcgccaa aaacgattgc ccgagttgga
      421 caggcctgct gacaagccgt caccaaatcg ccgtctcgaa ttggccgttt gtccattcgc
      481 gccgcaatac gcgctgcact gatccactgc acacaatagg tgcatttctc gatcacgccg
      541 cgccagcggg cagtgacgtc agggttgtgg atctcgtccg gagtcttggg agcccgttgg
      601 tggtagttga accaattgaa acgccggact ttgtagggac agttgttcga gcatgtccgc
      661 gtgccgacac accggctgta gaccatttga ttgagtccct cgctggagtg tacggtcgcg
      721 ttcacagggc aaacaatttc acaaggcgct ttttcgcaat gcatgcaaag cacgggttgg
      781 aagaacgtct cgggctcgtc tgagcctcct cggtagtatc ggtcgatgcg cagccagtgc
      841 atctcgcgtc cacgggagac ctcttccggc ccgacgaccg gcacattgtt ttcggcttgg
      901 caggcgataa cgcacgcatt gcacccgagg cagagatcga gatcgatggt catgccccag
      961 gcatttccat cataaatgta gcttggatag agcgaggcgc cagccggctt cgtagctttg
     1021 gccgcctccc cgggagcaac cacctggacg atattgcgtc cttccattcg gtggtgatgc
     1081 tgggtagtga tcagctcttt gctgcggccg gtagcctcta cgattacgtc cttcagaatc
     1141 cagggatgtt tcgagcttcg cagacgatat gcgtcaaacc cattcaagtc cccgatcata
     1201 ccggcatttc ggcggccgta cccaagaggc agcgtgacag cattcggcgc atggccaggc
     1261 agcagccaga ccggcgcttc aatgctcagc tctccttttg aaaaaacggc aatatcgccc
     1321 gtccgcagcc gataagcggc cgccgcagcc ggtgccatca gagctgcgtt tccccaaacc
     1381 tgcttggtga gtggcttggg aagctcctgc agccagccat tgttggcaaa gcggccgtcc
     1441 caaactgagg gatccggagc aaaaacgacc gtcggatttc cagaggattg cgcgatttgc
     1501 ggctgccttg gagaccagtt cggcacagct gctacgggga ccgagggcga cgcgctattc
     1561 gcgacgatgc ccgcctcgag cgcacttcgc cagcgagact caaatgattc gttccaagct
     1621 tcgcgccatg tagcctggac caaaacaaag tcaggttcag tgatgcctac gatccgggcc
     1681 aaaacctcct cggccgacat ggcttcgatg agtggcaccg tggccggctg gcataggccg
     1741 accgttccgt caaaggcccg cagatcgccc cagctttcga gcgcatggcg cgccggaata
     1801 tgccacaagg acgctcttga cgtttcatcg agccagtagc cgagatgcac cgaaagtgga
     1861 acccggccga tcagctccgc aaactcgagg tcgccgggcg cgtcataggc aggattgcag
     1921 ccgagtatta ggagcgcctc gacttggccc tcgacaattg cctccgctaa agacatgagc
     1981 ggctgcgcat aggctgcatc gggcgtgagc gggtcgatgt atcggacggt ccgaccgaca
     2041 gcacccagct cggcattaag agcatgcgca atcgcttgca caacggacgg ctgctcgtcc
     2101 cccgagaaga ttaaggatcg cggaccagcc cgtgcgatct ctcccgctat gaaatccaca
     2161 aaggtcttcg attgaggggg tgaccgctcg cgttgccgaa cgcgggaaaa tacttgtcga
     2221 gctattattt ctgcctgagc tggtgggacg gcgagccgac gatcagcacg cgcacctgtc
     2281 aggctgggcg tggtttcgac gctgtaaagc ctgggtagcg ggcgatcggg ggtgcgccgg
     2341 cgctgagcgt aatcggcagc ataccggacg tgagcaggcc cttggcccag cagatcaccg
     2401 ccgaacgtga gaatgacatc cgcctgagtc agatcgaaca ccggatcgac tggccgcccg
     2461 aaaacatcgg ccactgcatc gcggactgtt tcagatgcaa gtggcgaata gacgtaccat
     2521 ctcgcctcgg ggaacacgtg ctgcacatgt gcgaggagcc gatcgc
//
LOCUS       DASUMG010000171         2723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_744870, whole genome shotgun sequence.
ACCESSION   DASUMG010000171 DASUMG010000000
VERSION     DASUMG010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744870"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..927)
                     /locus_tag="VFX46_02780"
     CDS             complement(235..927)
                     /locus_tag="VFX46_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEX5599538.1"
                     /translation="MSNSPQQLTLELPHRPAHGAEDFLVSGCNEAAVLAIDRWPDWPH
                     PAMAVAGPAGSGKSHLVNVWRTRSNALMLPATALSQDSASEISAHRAIAIEDIDRGIA
                     DEQALFHILNMAAEHRLSVLLTSRHAPGELTIALPDLRSRLRAVPLVSIASPDEALLR
                     ALLVKLFADRQLAVEPHVISYLVLRMERSTEAAQRTVAEIDRLALATRRRVTRVLAAE
                     VLARLTPDEAGA"
     gene            complement(924..2030)
                     /locus_tag="VFX46_02785"
     CDS             complement(924..2030)
                     /locus_tag="VFX46_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5599539.1"
                     /translation="MRVERQVLFWLAALILLILAALVLRSVLLPFVAGMVIAYALNPL
                     ADRLGALGLPRVWASAIVVVLLIMVLVAAVVFLVPIIARQVQQLVEVLPGEIERVRGM
                     VEDSARERLGSHFADFQAWLDSVAAELSRNWASVIGFLAQSVWSQGRALVSFASLLLI
                     TPVVVFYLLVDWHSMLQKIDSWLPRDHAPTIRRLAIEINAAVSAFIRGQGLICLILGS
                     LYAVGLTWADIPYGLIIGLAAGLLSFVPFVGFALGLITALTVALIQYWPETLPLLKVL
                     AVFGIGQALDSSLLSPRIVGPKVGLHPVWLIFSLFVFGYLFGFVGVLVAVPMAAAIAV
                     LVRFALEVYLSSSIYKGRTALAPADLDQPLEKGP"
     gene            complement(2127..2681)
                     /locus_tag="VFX46_02790"
     CDS             complement(2127..2681)
                     /locus_tag="VFX46_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX5599540.1"
                     /translation="MSIPNLITLGRVILVPIVFWLLVSGRIQAAFVLFVVAGISDAID
                     GFLAKRFNWQTELGAYLDPLADKMLVVSIFVALGVLGELPSWLVIAVVSRDILIVMAV
                     VLSWLLDNPVRIKPLVVSKINTVTQIVLAAAVLADEGFGLGIGDIRTALVWAAAASTL
                     ASLVAYMRAWLAHMAGYQDENTTA"
BASE COUNT          589 a          872 c          842 g          420 t
ORIGIN      
        1 cggttcagcg cctcctccag cacccgccgg ttgaactgca accaagaaag ctcacgattg
       61 tagagcctgg cagggccttt ggggggctcg ggatggctag ctgtcctttc agcggcgttc
      121 tcgaccgcgc tcatcgacat ctcctgacac cgtgcgcaag ctgttggtct cggcacggaa
      181 cactacatgc agacccgtta cgataggatg atgcgggtcg cgcgggctag ccgttcacgc
      241 tccggcctca tccggcgtca gtcgggccag aacctctgcc gcgagcactc gggtcaccct
      301 tcttcgggtg gccaatgcga gcctgtcgat ctcggctacc gtgcgctgag ccgcctcggt
      361 cgagcgctcc atgcgcagca ccaaatacga aatgacatgc ggctccacgg cgagctgccg
      421 atcggcaaac agcttgacca gcaaggccct gagtagcgcc tcgtctgggc tcgcgatgct
      481 gacgagcggc acggccctga gccgcgatcg caaatcgggc agcgcaatgg tgagctcacc
      541 gggagcgtgc cggctggtca gcagcaccga cagcctatgc tcggcggcca tgttgagaat
      601 atggaaaagg gcctgctcgt cggcaatgcc gcggtcgatg tcctcgatcg cgattgcccg
      661 atgcgcactg atttccgacg cgctgtcctg gctcagcgca gtcgcgggca gcatgagcgc
      721 gttcgaccgt gttcgccaga cgttcacgag atggctcttg cccgagccgg cgggacccgc
      781 cactgccatg gccggatgcg gccagtccgg ccagcgatca atggccagta cagctgcctc
      841 gttgcacccg ctgaccagga aatcttccgc cccatgcgcg gggcggtgcg gaagctcgag
      901 cgtgagctgt tgagggctgt tgctcatgga cccttctcga gcggttgatc aaggtctgcg
      961 ggcgccaatg ccgtccgccc cttgtagatg gagcttgaca gatagacctc gagcgcgaag
     1021 cgaaccagca cggcgatcgc agccgccatc ggaaccgcga ccagaacgcc aacgaagccg
     1081 aacagatagc cgaacacgaa cagagagaaa atcagccaga ccggatgcaa gccgaccttc
     1141 gggccgacga tgcgcggcga cagcagactg gagtcgagcg cctggccgat gccgaacacg
     1201 gccaacacct tcaacagcgg cagcgtctcg ggccagtact ggataagtgc caccgtaagc
     1261 gcggtgatca gaccgagcgc aaagcccaca aacggcacga acgataagag acctgccgca
     1321 aggccgatga tcaggccgta gggaatatcg gcccaggtca gtccaacggc atataggctg
     1381 cccaatatca ggcagatgag cccctgaccg cggatgaacg cggagaccgc cgcattgatt
     1441 tcgatcgcca gtcgacggat cgtcggcgca tggtcgcgcg gcagccaact gtcgatcttc
     1501 tggagcatcg aatgccaatc gacgagcaaa tagaagacga ccaccggcgt tatcaggaga
     1561 agtgacgcga aactcacgag cgcccggccc tgcgaccaca ccgattgggc caaaaagccg
     1621 atgacactgg cccagttccg cgacagctcg gccgcgacgc tgtccaacca agcctggaaa
     1681 tccgcaaagt ggctgccgag acgctcccgc gccgagtcct ccaccattcc gcgcacacgc
     1741 tcaatctcac caggcaggac ttccacgagc tgctgcactt ggcgggcgat gatcggcacc
     1801 aggaagacca cggccgctac gagcaccatg atcaacagaa ccacgacgat ggccgaggcc
     1861 catacacgcg ggaggcccaa tgcgcccagg cgatccgcga gaggattgag agcataggcg
     1921 atgaccatgc cggccacgaa cggcagcagc acggagcgca ggaccagcgc cgcaaggatc
     1981 agcaggatca gcgccgcaag ccagaagagc acctgccgtt cgacacgcat gggatgcttc
     2041 cctcagcttg agcggttcgt ccccctgcga ccgcctggat gcgtggtttg tactaaggcc
     2101 gagagtgcaa ggattgcagc ttgatctcaa gctgttgtgt tttcgtcctg gtagccagcc
     2161 atgtgcgcca gccaggcccg catgtatgcg acaagcgacg ccaaggtcga tgcagcggca
     2221 gcccatacca gcgcggtgcg tatatcgccg atgccgaggc cgaacccctc atccgccagc
     2281 acggcggcgg cgagcacgat ctgcgtcact gtgttgatct tgctgacgac gagcggcttg
     2341 atccgcaccg ggttgtcgag cagccacgac agcacgacgg ccatcacgat caggatgtcg
     2401 cgcgaaacca cggcgatcac cagccaggac ggcagctcac ccagcacccc gagcgcgaca
     2461 aagatcgaga ccaccagcat cttgtcggcc agtggatcaa ggtaggcgcc cagctccgtc
     2521 tgccaattga agcgcttggc gagaaatccg tcgatggcgt cgctgatccc ggcgacgaca
     2581 aacagcacga acgcagcctg tatgcgtcca gagacgagca gccagaagac gatggggacg
     2641 aggatgaccc gtccgagagt gataaggtta ggtatgctca aggcgagatc cggtgggtct
     2701 cgggcatgtc aatcgttgta att
//
LOCUS       DASUMG010000172         4402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_343635, whole genome shotgun sequence.
ACCESSION   DASUMG010000172 DASUMG010000000
VERSION     DASUMG010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343635"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1329)
                     /locus_tag="VFX46_02795"
     CDS             complement(259..1329)
                     /locus_tag="VFX46_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599541.1"
                     /translation="MSTPYNVLILGASYGSLLATKLLLAGHNAKLVCLPAEVQLINAE
                     GTIVRMPAKGREGLVEVRSRDLPGKLSADGPDAIDPSGYDLVALAMQEPQYRSPSVRD
                     LLDRIAKARIPCMSIMNMPPLTYLKRLPGLDVEACRSAYTDPAVWDNLDPALMTLCSP
                     DPQAFRPPEENVNVLQVRLPTNFKAARFESDAHTDILRKLQADIEASRFEIDGTKVEL
                     PVKLKVHDSAYVPLAKWAMLIAGNYRCVLEDTVRPIKEAVHSNLDESRAVYNWVVELC
                     VKLGASESDMVPFEKYANAALSLLSPSSAARALAAGAPYIERVDRLVQIIAAQKSMKS
                     EAVDRTVALVDSWLERNRQKAS"
     gene            1608..2786
                     /locus_tag="VFX46_02800"
     CDS             1608..2786
                     /locus_tag="VFX46_02800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599542.1"
                     /translation="MPLLLVLALGVFAGALSMRVIDPVVPEVARDLDTTTSTVALLAS
                     AFSFPYAFSQPLLGPLADSMGKARIIKIFLGVLTVAMLISALAPSIEALFVARVVAGI
                     AAGGVIPVAFAIVGDRFDLADRHLALSRIILAAVTGQLTSSIGAGFIAAVFGWRVALA
                     ITGLVALATLVASILYLSPRPKVERPQFTLSGLRSAYATIFADPRAYVCYGAVATEGL
                     IVYGMAPYVATLLEAKGLGGIREAGIVIAGIGIGGVIYAALVRILLKWLGGKFNIMRG
                     GGVIAASGFCLISLQPIWPLQFAGFVLVGLGFFMIHNVLQVQATELAPNARGSAIALH
                     SFFFFLGQAAGPVAYGTGFSTLGVLMTVLLAAAIMLTMGFTSAGALQRLEARLPVRDS
                     "
     gene            complement(2912..>4402)
                     /locus_tag="VFX46_02805"
     CDS             complement(2912..>4402)
                     /locus_tag="VFX46_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX5599543.1"
                     /translation="EMVAKMAQKPLILALANPTPEILPEDARAVRPDAVIATGRSDYP
                     NQVNNVLCFPFIFRGALDVGATTINEEMKKAAVLALARIVEAEASDIVLSAYRADTFV
                     FGPDYILPKPFDPRLMTEIAPAVAKAAMETGVATRPIEDFEAYKERLSSFIYQSGFVM
                     RPIMEAAKLAHRESPRRIVFAEGEHPYVLQAAYQVIAEGIARPILVGRRENILRMLPH
                     LGLRMRPEVDFDIFDQETDPRVEVMTEEYHRLVERRGISPSHARRVVRAGSTVIAGLL
                     LRRGDADAMICGAVGRYQTQLRHVSQVIGKRKDVRGLAALSGIILPSGPLFMADTYVN
                     YDPDPQHLAEITLLAAEEVRRFGINPKVALLSHSNFGSEDTSSALKMREVLAILNERA
                     PDLEVEGEMHADAALSPFIREEIFPNSRLTGSANLLIMPCLDAANIAFNMLKVVSGAV
                     VLGPILLGAEKAVHIVTPSITVRGLLNMSAIALVDSFRSGGPQSAR"
BASE COUNT          802 a         1337 c         1355 g          908 t
ORIGIN      
        1 gccgaacaag gttggcgccc aatccaagct cgcaagaacc gatcagcctt agcccaagtg
       61 gctgtcagcg tacgagcgat gaatgtggaa acggcagttg gctgtcgcaa ccaacggtag
      121 cccaaaccaa aggaggcatc ctctcctgcg ggggtggttc gggcaattga tcgattccag
      181 atcgtgcaac tcgcctcccg cctcggggga ggcggtcgag gtgcctcgtg gtcgtgggca
      241 cctcgaccaa aacgtggctt agctcgcctt ctgccggttt cgttcaagcc agctgtcgac
      301 caaggccacc gtacggtcca ccgcctcgga cttcatgctc ttctgcgcgg cgatgatctg
      361 tacgaggcgg tcgacgcgct cgatgtacgg agcgccggcg gcgagcgcac gggcggccga
      421 tgacgggctc aagagcgaca gggcagcgtt ggcgtacttc tcgaacggaa ccatgtcgct
      481 ctcgctggcg ccgagcttca cgcagagctc gacaacccaa ttgtacacgg cccgcgattc
      541 gtcgaggttc gagtgaacgg cctccttgat cggccgcacg gtatcctcaa gcacgcagcg
      601 gtagttgcca gcgatcagca tggcccactt cgccagcggc acatacgcgg agtcgtgcac
      661 cttgagcttg acgggaagct cgaccttggt gccgtcgatc tcgaaccgcg aagcctcgat
      721 atcggcctgg agcttccgca gaatgtctgt atgtgcatcc gactcgaagc gggccgcctt
      781 gaagttcgtc ggcagccgga cttgtagcac gttgacgttc tcctcgggcg gacggaaggc
      841 ctgcgggtcg gggctgcaaa gcgtcatcaa agcagggtca aggttgtccc agaccgccgg
      901 atcggtgtag gcgctccggc aggcttcgac gtcgagacct ggaaggcgct tcaagtaggt
      961 gagtggcggc atgttcatga tcgacatgca ggggatccgg gccttcgcga ttcggtcgag
     1021 caggtcgcga acgctcggcg agcggtactg tggctcctgc atggcgagcg ccacgagatc
     1081 gtatccgctc ggatcgatgg cgtcgggtcc gtcggccgac aattttccgg ggagatcccg
     1141 agatctcacc tccacgaggc cttcccggcc tttagccggc atgcggacga tcgtgccctc
     1201 tgcgttgatg agctgtacct cagctggaag gcagaccagc ttggcattat gaccggccag
     1261 cagcagcttg gtcgctagca gtgatccgta agaagcacca aggatcaaga cattgtaggg
     1321 cgtgctcatc tgaagggccc tccgctcccg attggttggt tttgccaggg ggcgggaagc
     1381 ccgccccaaa tccgtgggca tgctagagag ttaacgcccg tgacgcaatc cgtggctcaa
     1441 tcgacaggcg ctactgcacg ttccgcatac ctgatcatca ctacaaatgc tgcattcgcg
     1501 ctcgacggtt tgatccgtcc ggcttaccgt tgttcttgtc ttaagttgtc tgcagcggct
     1561 gggggagtct ctgcaggcag ccatccgcta ggtcctcgac gctgccgatg cctcttctcc
     1621 ttgtccttgc gctgggggtc ttcgctggag cgctgtcgat gcgcgtgatc gaccccgtgg
     1681 tgccggaggt tgctcgcgat ctggacacga cgacttcgac ggtcgcgctg ctcgcatcgg
     1741 ccttctcatt cccttacgcc tttagccagc cgctgctggg gccgctggcg gactcgatgg
     1801 gcaaggcgcg catcatcaag atttttctcg gcgtgcttac cgtcgcaatg ctcatctccg
     1861 cgcttgcccc gtccatagag gcgctgtttg ttgcgcgcgt agtggcgggc atcgcggccg
     1921 gcggtgttat ccccgtcgcc ttcgcgattg ttggtgaccg gttcgatttg gccgaccgcc
     1981 acctcgccct ttcacggatc attctggctg cggtgacggg gcagctcacg agctccatcg
     2041 gggctgggtt catcgcagca gttttcgggt ggcgtgttgc tctcgcgatc acgggcttgg
     2101 ttgcgctggc gacgctcgtt gcgagcattc tctacctttc acctcggcca aaggtggagc
     2161 ggccgcaatt caccttgagc ggcctgagat cggcatacgc gacgatcttc gctgatccgc
     2221 gggcgtacgt ttgctatggc gcggtcgcca ccgaagggct cattgtctac ggcatggcgc
     2281 cgtatgtcgc gacgttgttg gaggccaagg gacttggcgg cattcgcgag gcaggcatcg
     2341 tcatcgccgg catcgggatc ggcggtgtca tctacgctgc cctggttcgt atcctgctga
     2401 aatggctcgg cggcaaattc aacattatgc gcggaggcgg cgtgattgct gcctcgggat
     2461 tctgcctcat ttcactgcag ccgatctggc cactgcagtt tgccgggttc gtcctcgtcg
     2521 ggctcggctt cttcatgatc cataacgtgc tgcaggtgca ggcgacggag ctggccccaa
     2581 atgctcgagg ctcggcaata gctctgcact cgtttttctt cttcctgggg caagcagcgg
     2641 ggccggtagc ctatggcaca gggttctcga ctctgggcgt tctgatgact gtgcttttgg
     2701 ccgccgcaat catgctgacc atgggcttca cctccgcagg cgcactgcag cggctggaag
     2761 cccgcctgcc agtccgtgat tcgtaagccg aattcttatt acatgcagaa acacgggtcg
     2821 ccgcctgcac cctgaagcgt tgcctgcgta gcgaattatt gaacccaaat tgaatcgaca
     2881 gagctgcagt ttgtcagcgt gtaccagtgc tctagcgcgc cgattgaggt ccacctgacc
     2941 tgaaactatc aaccaaggcg atggcactca tattgagaag accacggacg gtaatcgaag
     3001 gcgtcacgat gtgaacggct ttctccgctc cgagaagtat cgggccgaga accacagctc
     3061 cggacaccac cttcagcatg ttgaaggcga tattggctgc gtcgagacat ggcataatca
     3121 gcaagttcgc cgagccggtg agacgcgaat tcgggaaaat ctcctcgcga ataaatggcg
     3181 aaagcgccgc atcggcatgc atttcgcctt ccacctcgag atccggtgcc cgttcgttca
     3241 ggattgcgag cacttctcgc attttcagcg ccgaggaggt gtcctccgaa ccgaaattag
     3301 agtgcgagag gagcgctacc ttgggattga tgccgaaacg gcgcacttcc tccgctgcga
     3361 gtagcgttat ttcagccaaa tgctgcggat ccggatcata attgacgtag gtgtcggcca
     3421 tgaacagcgg accggatggc aggataatgc cagaaagcgc cgctagtccg cgcacatcct
     3481 ttctcttgcc gatgacctgt gacacatgcc gaagctgggt ctgatagcga cccacggcgc
     3541 cgcagatcat cgcgtcggca tcgccgcgcc tcaacagcag gccagcaata accgttgatc
     3601 ccgcccgtac gacacggcgg gcatggcttg gagaaatgcc ccggcgctcg acgaggcgat
     3661 gatattcctc ggtcatcacc tcgacccgag ggtcagtctc ctgatcgaaa atgtcgaaat
     3721 cgacctcggg ccgcatcctc agccccagat gcggcagcat gcgaaggata ttttcccgcc
     3781 ggccgacgag gatggggcgc gctatccctt ccgcaatcac ttgataggcg gcctgaagga
     3841 cgtaggggtg ctcgccctcg gcaaacacaa tgcgccgcgg cgactcgcga tgcgccagct
     3901 tcgcggcctc catgatcggc cgcatgacga agccggattg ataaataaag ctcgagagtc
     3961 gctccttgta cgcttcgaaa tcctcaatcg gccgcgtggc aacgccggtc tccatcgccg
     4021 ctttggccac cgctggcgca atctcagtca tgagccgcgg atcgaacggc ttcggaagaa
     4081 tgtaatccgg cccgaacacg aacgtgtccg ctcgataggc tgagagaacg atatcggaag
     4141 cctcggcctc gacgattctg gccagggcca agacggccgc cttcttcatc tcctcattga
     4201 tcgtcgtcgc tccgacgtcg agcgcgccgc ggaagatgaa ggggaagcag aggacgttgt
     4261 tgacctggtt gggatagtcc gagcggccgg tcgcaatcac cgcatcgggc cgcaccgcgc
     4321 gcgcgtcctc gggcaggatc tccggggtgg gattggccag cgccaggatc agcggcttct
     4381 gggccatctt cgccaccatc tc
//
LOCUS       DASUMG010000173         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_22139, whole genome shotgun sequence.
ACCESSION   DASUMG010000173 DASUMG010000000
VERSION     DASUMG010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22139"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFX46_02810"
     CDS             <1..742
                     /locus_tag="VFX46_02810"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5599544.1"
                     /translation="LGIVGAIKVEASPWVEDNLWVLEVAERDTIIVGVIGNLEPDKPD
                     YGEMLSRYHKNRLFRGIRYGNLWGRDITRQADNPAFIEGLKLLAQADLVLDTANPRVD
                     LLQAIVRITDKVPDLRVVVDHLPSLEPTPQSQQAYEEAIRELQQRPRVFVKLSAVIHR
                     VNGVVATDLAPYRDRLNRLVGTFGEDRILFGSDWPNSDGVAPIDKVVGIVREFFQTQP
                     RGVSEKYFWKNSLAAYKWIKRDPMQPAI"
     gene            complement(749..1315)
                     /locus_tag="VFX46_02815"
     CDS             complement(749..1315)
                     /locus_tag="VFX46_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5599545.1"
                     /translation="MTHLNIRSVGLVSMLALCIAVPAQAQRREARNPHQGSSAVLGEV
                     GLFLPTQDGMTTGPAVEGAYEYYLTARNSLRLGVGWANPKLDREHSDGTRQIRFGGDL
                     IHNWEGGAVHPFVGAGAGSYFLQPLDNGDSFGPSNTRFGARFLGGVEYFTSRSFAIKG
                     EGRYDAVMKANGYNPSGLTFSIGVKSYF"
     gene            complement(1558..>2213)
                     /locus_tag="VFX46_02820"
     CDS             complement(1558..>2213)
                     /locus_tag="VFX46_02820"
                     /inference="COORDINATES: protein motif:HMM:NF018534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599546.1"
                     /translation="NFDRRPLRNSSGVTGAGPIFHAVMVAAERRAAAGDARMLPEPSP
                     QAEVGVQAVEICALSGMAANSWCPARRREWIPTENGLPPCNWHHLTDEGVLTVWPAEY
                     RQWAIDHAETESNDRLGEVAAIRISGDASLKTLRISNPPDGATYLIDPTLRREFQSLS
                     LRAVLPVPGQVEWTVDRRPLATSASEQSVAWPLAPGRHTFVARTADGRSAESTITVR"
BASE COUNT          438 a          667 c          688 g          420 t
ORIGIN      
        1 gctcggaatc gttggcgcca tcaaagttga agcaagcccc tgggtagaag acaacctctg
       61 ggtgctcgag gtcgccgaac gcgacactat catcgtcggc gtcatcggca acctggagcc
      121 cgacaagcct gattacgggg agatgctgtc gcggtaccac aagaatcgtc tatttcgcgg
      181 gatccgatac ggcaacctgt gggggcgaga catcaccagg caggctgaca accctgcttt
      241 catcgaagga ctgaagctgc tcgctcaggc cgatctggtt ctggacacgg caaatccccg
      301 cgtcgatctg ctgcaagcca tcgtgcggat caccgacaag gttcccgatc tccgagtcgt
      361 cgtcgatcat ctgccgagcc tcgaacccac acctcagtca caacaagcat atgaggaggc
      421 gatccgtgag cttcaacagc gtccgcgagt cttcgtcaag ttgtcggccg tcatccaccg
      481 cgtgaacggc gtcgtggcaa cagacctggc gccgtatcgc gatcgtttga atcgcttggt
      541 gggcacgttt ggagaagatc ggattctctt cggcagcgac tggccgaata gtgacggggt
      601 cgcgccgatc gacaaagtag tggggatagt gcgggagttc ttccagacgc aaccgagagg
      661 ggtctcggaa aaatatttct ggaagaactc cctcgctgcc tacaagtgga tcaagcgcga
      721 tccgatgcag ccagcgatat gaggtgcgct agaagtagct cttcacgcca atggagaacg
      781 tgaggcccga tgggttatag ccattggcct tcatgactgc atcgtagcgg ccttctccct
      841 tgatcgcgaa cgatcgagac gtgaagtatt cgacgccgcc caggaaacgc gcgccgaatc
      901 tcgtgttgct cgggccgaag ctgtcgccgt tgtcgagtgg ctgcaggaag taggagcccg
      961 ctcctgcgcc aacaaacgga tggacggcgc ctccctccca gttgtggatg aggtcgccac
     1021 caaaccggat ctgccgcgtg ccgtcggaat gttcacgatc gagcttcgga tttgcccagc
     1081 ccacgccaag gcgcaggctg ttgcgcgcgg tcaggtagta ctcgtaggca ccctcaaccg
     1141 ccggtcccgt cgtcatgccg tcctgagtcg gaaggaaaag accaacctca ccaagcacgg
     1201 cggatgagcc ttgatgcggg ttgcgagcct cccgacgttg agcctgggcc gggacagcga
     1261 tgcagagagc aagcattgat acgaggccta ctgaacgaat gttgagatgt gtcatctgta
     1321 tgcactcctc gagatacaag ccccgcaacc tcaatgccca taatgacctc agctgtggct
     1381 ggagccgtat ccccagtggg ttacaagatg accgctcagc tgagtgtcct attacggcgc
     1441 gcttatatgg ttgtgcttgg tgggcacgcg gcgtccgact tgtggacgtt agatgggatt
     1501 tctgaccggt tgctgaactc gaccgtgacc agcggtcgag cttctggctg gagcgcgtca
     1561 gcgcaccgtt atggtcgatt cggccgagcg gccatcggcg gtgcgcgcca caaaggtatg
     1621 acggcctggc gcgagcggcc aagcgacgct ctgctcggat gccgaggtgg cgagcggtcg
     1681 ccgatcgacg gtccactcga cctgaccagg aaccggcagc acagcgcgga gcgacagcga
     1741 ttgaaactcg cggcgcagcg tcgggtcgat cagataggtc gcgccgtcgg gtggattgga
     1801 gatccgcaga gtcttgagag acgcatcgcc gcttatgcgg atcgcagcga cttcgcctaa
     1861 gcgatcgttc gattcagtct cggcatggtc gatggcccac tgacgatact cagccggcca
     1921 aacggtgagc accccctcgt cggtcagatg gtgccagttg cacgggggca ggccgttctc
     1981 tgtcggaatc cattctcgcc gtcgcgccgg gcaccacgag ttcgccgcca ttccagacag
     2041 cgcgcagatc tcgaccgcct gcacgcccac ctctgcttgt gggcttggtt cgggcaacat
     2101 ccgcgcgtca ccggccgctg cccgccgctc cgccgcgacc atgacggcgt ggaaaattgg
     2161 acctgcgccc gtgacgccgc ttgaattccg cagcggccgg cgatcgaagt tgc
//
LOCUS       DASUMG010000174         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_343722, whole genome shotgun sequence.
ACCESSION   DASUMG010000174 DASUMG010000000
VERSION     DASUMG010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343722"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..315
                     /locus_tag="VFX46_02825"
     CDS             13..315
                     /locus_tag="VFX46_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEX5599547.1"
                     /translation="MSVVCFDRRELDDILAVYGRKVATGEWRDYAIDIGRDKAVFSIF
                     RRSSECPLYRIEKNPKLARKQGAYSVIAAGGLILKRGHDLRRVLGVLERRLRVVGH"
     gene            459..2291
                     /locus_tag="VFX46_02830"
     CDS             459..2291
                     /locus_tag="VFX46_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5599548.1"
                     /translation="MAPPLVALRDIHLTFGGTPLLTGAELSVGPGERVCLVGRNGSGK
                     STLLRIAAGLVEPDRGERFIQPGALVRYLPQEVDFSAFTTTLEVVEAGLSPTQDRYQA
                     RYLLEMLGLTGAEPVATLSGGELRRVALAQVLAPEPDVLLLDEPTNHLDLPTIEWLEA
                     ELQRSRAALVLISHDRRFLENLSRCTVWLDRGVTRRLEQGFGAFEAWRDEILEQEEIE
                     RHKLDRKIAREESWLHGGVTARRKRNVRRVAELARMRQERREARRAMGNVRFTVGESG
                     PSGRLVIEANGISKAFEGKAVVKNFSTRIARGDRIGIVGPNGAGKTTLLKMLTGQLEP
                     DAGEVRLGSGIEMVSLDQRRDELAPTMTVKEALAGKGDKVIINGQPRHVASYMKDFLF
                     QPEQAFSPVSVLSGGERARLMLARALAKPSNLLVLDEPTNDLDLETLDLVQELLAEYA
                     GTVLLVSHDRDFLDRVVTSVIAAEGDGQWTEYAGGYSDMLAQRSGVDLPSTVAAKPAA
                     PEAKAKPSASTAAGNAPQRKRKLSFHEKRALETLPGVIDALQAEIEKLQATLSDPDLY
                     TRDPKTFETATTRLTDAQAELADAEERWLELELLREELEGAGGN"
     gene            complement(2344..>2711)
                     /locus_tag="VFX46_02835"
     CDS             complement(2344..>2711)
                     /locus_tag="VFX46_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5599549.1"
                     /translation="GANDALRGVDPAETRRNLDEILTRLDARGIPTLLAGMMAPRNWG
                     DDYVTRFDAIYPDLAERHGALLYPFFIERVALRPELNLDDGMHPNSAGVAAIVEDILP
                     KVEELIARVEAKRDAAPAN"
BASE COUNT          509 a          811 c          883 g          508 t
ORIGIN      
        1 gctccagccc aggtgtctgt tgtctgtttt gatcgccgtg agctggacga catcctcgcc
       61 gtttatggcc gcaaggttgc cactggggaa tggcgcgact acgccatcga tatagggcgg
      121 gacaaggccg tgttttccat cttccggcgc tcgagtgagt gcccgctcta ccggatcgaa
      181 aaaaacccga aacttgccag aaagcagggg gcttacagcg tcattgccgc cggcgggctg
      241 atcctgaagc gcggacatga cctgcgccgc gtcctgggag tgctggagag gcggctaagg
      301 gtggtgggac actgagccac cttcggtagc ctggcagggt ggttgacgct tgcccacgct
      361 ggcctttggc cgcagatcac ccccggtgcg agaggagcca ttacactgct cctttttaca
      421 ggctcgactg gccatcacga atcggactga ggcattcgat ggcacctcct ttggtggcgc
      481 tacgcgacat tcatttgacg ttcggcggca caccgctgct gacgggggcc gagctgtccg
      541 tcgggccggg cgagcgcgtc tgtctcgtgg gccgcaacgg ctcaggcaaa tccaccttgc
      601 tgcgcatcgc agcggggttg gtagagccgg accggggcga gcggttcatt cagcctgggg
      661 ctttggtgcg gtatctgccg caggaggtgg atttttccgc attcacgacg acgctcgagg
      721 tcgtcgaagc tggcctgtca cccacgcagg atcgttatca agcgcgctat ctgctagaaa
      781 tgctgggcct gaccggggcc gagcccgtcg cgacgctttc cggtggtgag ttgcggcgcg
      841 ttgcgctggc gcaggtgctg gcaccggagc ccgacgtcct gctgctcgac gagccgacga
      901 accatctcga tctaccgacg atcgaatggc tggaggcaga actccagcgg tcacgcgccg
      961 ctctggttct gatcagccac gaccgccgct tcctggaaaa cctctcccgg tgcacggtat
     1021 ggctcgatcg tggggttact cggcgtttgg agcaaggttt cggcgctttt gaggcctggc
     1081 gcgacgaaat cctggaacag gaggaaatcg agcgccataa gctcgaccgc aagatcgcgc
     1141 gcgaagagag ttggttgcat ggcggcgtaa ccgcacgccg caagcgcaat gtgcgccggg
     1201 tggctgagct cgctcgcatg cgtcaggagc ggcgagaggc tcgccgcgct atgggcaatg
     1261 ttcgtttcac cgtcggggag agtggaccat cgggccgcct cgtcatcgag gccaacggca
     1321 tatcgaaagc cttcgagggc aaggcggtcg tgaagaattt ctcgacccgg attgctcgcg
     1381 gcgaccgcat cggcatcgtc ggccctaatg gcgcgggcaa gacgacattg ctcaagatgc
     1441 taacgggcca gctcgagccg gacgccggag aggtgcgcct cggtagcggc attgagatgg
     1501 tctcgcttga ccagcgccgg gatgagctgg cgccgaccat gaccgtgaaa gaagccctcg
     1561 caggcaaggg tgacaaggtc atcatcaacg gccagccgcg gcatgtcgca agctatatga
     1621 aagatttcct gttccaaccg gagcaggcct tcagtcccgt atcggtcctt tctggtggtg
     1681 agcgggcacg tcttatgttg gcgcgtgcct tagccaaacc atccaacctg ctcgttcttg
     1741 acgagccgac gaacgatctc gacctcgaaa cgctcgatct ggttcaggag cttcttgccg
     1801 aatatgccgg aactgtgctg ctcgtcagcc acgaccgtga ctttctcgat cgggtcgtaa
     1861 cttcggtcat tgcggccgag ggggatgggc agtggaccga gtatgcgggc ggctactccg
     1921 acatgctggc gcagcgctca ggcgtagatc tgccgtccac cgttgctgca aagcctgccg
     1981 cacctgaggc caaggcgaag ccatcagcaa gcacagccgc aggcaatgct ccgcagcgga
     2041 agcgaaagct atcgtttcac gagaaacgtg cgctcgaaac tctgccaggg gtcatcgatg
     2101 cgctgcaggc cgagatcgag aagctgcaag caaccctttc tgatcccgac ctttacaccc
     2161 gcgatccaaa aacgttcgag acggcgacta cccggctcac cgatgcgcag gctgagctgg
     2221 ccgacgccga ggagcggtgg ctggagctgg agctgctccg cgaagagctg gaaggtgcgg
     2281 gtggaaacta gctgttcctg ctaaccccga aagcgacttg gcggtcgaaa ttgagggcgg
     2341 caactagttg gcgggggccg catcccgctt tgcctccacg cgcgcgatca gctcctcgac
     2401 tttcggcaag atgtcttcaa caattgccgc cacgccagcg ctgttcgggt gcataccgtc
     2461 gtcgaggttc agctcgggcc gtagcgcgac acgctcgatg aaaaacggat acagcaacgc
     2521 gccgtgacgt tcggcgaggt ccggataaat ggcgtcgaac ctcgtgacgt aatcatcgcc
     2581 ccagttgcgc ggagccatca tgccggcaag cagagtaggg atgccgcgcg cgtcgagccg
     2641 ggtgagaatc tcatccaaat tgcgacgcgt ttctgccgga tcgacgccgc ggagcgcgtc
     2701 attcgcgccc a
//
LOCUS       DASUMG010000175         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_344109, whole genome shotgun sequence.
ACCESSION   DASUMG010000175 DASUMG010000000
VERSION     DASUMG010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344109"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFX46_02840"
     CDS             complement(<1..554)
                     /locus_tag="VFX46_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5599550.1"
                     /translation="MLRSVASAVVGVAATGAFLASPAFADGGYPIYGDGGFKDEVHFA
                     PPPITWTGFYIGFHAGYGWGDSDWKALDDFDPILAGDRFNLDPEGGVFGGYIGYNAQT
                     GPVVWGIEASLSGADINESVRTTFGGLIDPPPIRFDTDIDTLWSVTGRIGYDWGRLLT
                     YVKAGYAGADVELTVTDPLTFRIS"
     gene            complement(870..1535)
                     /locus_tag="VFX46_02845"
     CDS             complement(870..1535)
                     /locus_tag="VFX46_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX5599551.1"
                     /translation="MCNLYSQTRAAEAVRRLFRVSHNRCASFEPRAAIFPGHDAPVVR
                     RAADGEREIALLSWGFVLPQPNRAAKRVTNVRDDKVLTSPFWRESFLQRRCLVPASSF
                     CEPKGSRPATWHWFALCGDEPRPLFAFAGVWRQWSGPLKPGGPSVVLDVFSILTTRPN
                     ALVATINHERMPVLLRSEDEFETWLSGSPQEAFSLIRSVEADAMRIVRSGTDREDMVA
                     EQA"
     gene            complement(1735..>2132)
                     /locus_tag="VFX46_02850"
     CDS             complement(1735..>2132)
                     /locus_tag="VFX46_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5599552.1"
                     /translation="KELAEKRADSTVNNPSEQSDFYPKGLTKPIAAFTPQRLQMYLRT
                     PTFRENGMDFSYFMQRSVVGAPGMAPEAQEYYIRLFKRVYDTPEWQDYMKKNSLFGEF
                     LSGPELMKYWLDQREKHARWKMALQVMKR"
BASE COUNT          441 a          655 c          634 g          402 t
ORIGIN      
        1 tccgaaattc ggaatgtgag cgggtcggtg acggtaagct cgacatccgc tccagcatat
       61 ccggccttca cataagtgag caggcggccc caatcgtatc cgatccgacc tgtcacgctc
      121 cacagcgtgt cgatgtctgt atcgaagcgt atcggaggcg gatcgatcag cccgccaaaa
      181 gtcgtgcgca cgctctcgtt gatgtcagct ccagagagcg aagcctcgat gccccaaaca
      241 actgggccag tctgagcatt gtacccgatg tacccgccga aaaccccgcc ttcggggtca
      301 agattgaaac ggtcgccagc caatatgggg tcgaaatcat cgagggcctt ccaatcagag
      361 tcgccccagc cgtagccggc atgaaagccg atgtagaagc cggtccatgt gatgggcggg
      421 ggtgcaaagt gcacttcgtc cttgaagcca ccgtcgccat agatcgggta cccgccgtcg
      481 gcaaatgcgg gcgatgctag gaatgctccg gtggccgcta cgccgacaac ggccgatgca
      541 actgacctca acactgaaag tccctccata cagcagagct tgctgagcta ctgacaattg
      601 gcccttgggg accactccct acggtccgtg gtctgattcg tactccgacg cgaactgagc
      661 gatcaacgca accactgcta gaatagaggg caagtcttaa gcatgcgtta ggtctgagcg
      721 gatcagcgcg ccgagaaatc agtaaggaca ggcacttggc gggtgttgca gcgcggtcgc
      781 agtggcggag atacgactac tggttgtgta aaacgcgact atcaacagct ttaccgcgag
      841 ccccgagcag cgatttgaac gcctgcgact tatgcttgct ccgccaccat gtcctcccgg
      901 tcggtacctg accgcacgat gcgcatggcg tcggcctcga cggagcgaat gagcgagaat
      961 gcctcttggg gtgaaccgga gagccaggtc tcgaactcat cctcgctcct cagcagaacg
     1021 ggcatccgtt cgtggttgat cgtcgccacg agtgcattcg gccgcgtcgt gagaatgctg
     1081 aacacatcca gcacgacgga gggccctcca ggcttcaacg ggcctgacca ctgccgccaa
     1141 accccagcga aggcgaacag cggtcgcggc tcgtccccac acagcgcgaa ccagtgccat
     1201 gtggccggtc gcgatccctt cggctcgcag aaggacgacg ctggcaccag gcagcgccgt
     1261 tgcaggaatg actcgcgcca aaaggggctc gtcagcacct tgtcgtcacg aacgttcgtc
     1321 acgcgtttcg ccgcgcggtt gggctggggg agcacaaagc cccaggacag gagcgcgatc
     1381 tcccgctcgc catccgctgc cctgcggacg acgggagcgt cgtgtccggg aaagatggct
     1441 gcgcgcggct caaatgacgc gcaccggttg tgagacactc gaaaaagcct gcgcacggcc
     1501 tccgccgcgc gggtctggga gtagagattg cacatgcaaa aatactagca atctctgagc
     1561 tgctcgccga gccgccataa gcgcgcgctg gacgaaaatc agcttcggga tcgatatcct
     1621 accgtcacca tcagccggta ttcagagcga gctgcgaagg cggacgctcg cccgcggatc
     1681 gatggtgacc agtgaaaccc tcataatctc tgccgaccgg cggtcgctga ccgtctaccg
     1741 cttcatgacc tgtagggcca ttttccagcg ggcgtgcttc tcgcgctgat cgagccaata
     1801 cttcatcagc tccggcccag acagaaattc gccgaacagg ctgtttttct tcatatagtc
     1861 ctgccactcg ggtgtgtcgt agactcgctt gaacagccgg atgtagtact cctgcgcctc
     1921 cggggccatt cctggagcac ccaccactga gcgctgcatg aaatagctga agtccatgcc
     1981 attttctcgg aaggtcgggg tgcgcaagta catttgcagg cgctgcggcg tgaaggcggc
     2041 gatcggcttg gtcagccctt ttgggtagaa gtcactttgc tcggacgggt tgttcaccgt
     2101 cgagtcggcg cgcttctccg cgagctcttt tg
//
LOCUS       DASUMG010000176         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_22914, whole genome shotgun sequence.
ACCESSION   DASUMG010000176 DASUMG010000000
VERSION     DASUMG010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22914"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VFX46_02855"
     CDS             <1..1679
                     /locus_tag="VFX46_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX5599553.1"
                     /translation="GDGTKGWPQAAPFNAILVAAGSPVVPPALKEQLAIGGRLIIPIS
                     GPTHEVLTRIRRTGEDTFQEEEHGLVAFVPLVGAGEWPPMVENSAQTPGAPLGAPSAV
                     GDPQVVTLGPVGAVTELALETGYRPTSNIGYELRQAALPFDLMTDLSGIATGLIGDRR
                     IVMLGEATHGTAEFYDARAEITAKLVAKHGFNIVAVEADWSDAAAYNRIIHGQPAVEP
                     GLSSPFTRFPRWMWRNTEMFHFLTRLRDLNREGRAQVSFYGLDIYGLNGSIEAVLHYL
                     DRVDPAAAAVARERYGCLTPWRADPVEYARMAASSTYHSCEDEATRMLVDLLEKRVEY
                     MKRDGSAFFDAEQNARVIADAEKYYRTMYRGSAESWNLRAQHMFETLERLLDHHGPGS
                     KAVVWAHNSHIGDARATEMGQVRGEHNLGQLARERWGDEVALVGFGTGSGFVAAASHW
                     DGELEIKRIRPPRSGSYEACCHDTGLPSFVLSLLPDQRREVIEILSEPRLERAIGVIY
                     RPETELISHYYRAVLPRQFDVWVWFNETGPVTAIPVPGVTDERETYPFGL"
     gene            complement(1786..>2580)
                     /locus_tag="VFX46_02860"
     CDS             complement(1786..>2580)
                     /locus_tag="VFX46_02860"
                     /inference="COORDINATES: protein motif:HMM:NF016999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEX5599554.1"
                     /translation="VLPMVLVALLTIVAGFIAAAVLMRLADMDVVAAGLASIPGGPVE
                     MAALAEHHNVPPAPIALAQTLRISLLVLIIPPVLVAISGGAPNAALTVGERSLPGMLL
                     LFALAVAGGLLFRWLRITSPFFLGALGFNAAATAFALPVSMPPYWVLAGAQILLGVWL
                     GCMFDRETLARTRAFVLAAIVSTGVLIVLCAGMAFAIAAVTGIDWHTMILATAPGSVT
                     EMALTAKILNQQVAIVTAFHIVRIFIILPFLPLIFSVTAKLANRSR"
BASE COUNT          528 a          766 c          843 g          443 t
ORIGIN      
        1 taggcgatgg cacgaagggc tggccccagg ctgccccgtt caatgccatt ctcgtggctg
       61 caggtagccc ggtggtgccc ccagcgctga aggagcagct tgctatcgga ggccgcttga
      121 tcatccccat cagcgggccg acgcacgagg tgctgacgcg gatccggcgg acgggcgaag
      181 acaccttcca ggaagaagaa cacggcctgg tagcgttcgt accgctggtc ggcgcgggag
      241 aatggccacc catggtggag aacagtgccc agacgcctgg agcacctttg ggcgcgcctt
      301 cggcggttgg tgatccgcag gtcgtaacgc tcggtcccgt cggagctgtg accgagctcg
      361 ctctcgaaac gggttatcgc cccaccagca acatcggcta cgagctgagg caggccgcac
      421 tgccgttcga cttgatgacc gatttgagcg ggatcgccac aggcctcatc ggcgaccggc
      481 gcatcgtcat gctcggcgag gcaacacatg ggacggcgga gttctacgat gcgcgtgccg
      541 agattacggc aaagcttgtc gcgaagcatg gattcaacat cgtcgccgtc gaggcggatt
      601 ggtccgatgc cgcggcttac aatcggatca tccacggcca gcctgccgtc gagccgggat
      661 tgtcgagccc gttcacacgc ttcccacggt ggatgtggcg caacaccgaa atgtttcatt
      721 tcctgactcg cctgcgggac ctcaaccgcg aaggcagggc gcaggtcagc ttctatgggc
      781 tggacattta cggcctcaac ggatccatcg aagccgtgct gcactatctc gatcgcgtgg
      841 atccggcagc ggcggccgtc gcgcgcgagc gttacggatg cttgacgccg tggcgagcgg
      901 acccggtgga atatgcgcgc atggccgcca gcagcaccta ccactcgtgc gaggacgaag
      961 cgacgcgcat gctcgtcgat ctcctcgaga aacgcgtgga atacatgaag cgcgacggct
     1021 cagccttctt cgacgctgag cagaatgcgc gcgtgatcgc cgacgcggag aaatactacc
     1081 ggaccatgta ccgcggctcg gcggagtctt ggaacctccg tgcccagcat atgttcgaga
     1141 cgctcgaaag gctgctggat catcatgggc ccgggagcaa ggccgtggtg tgggcgcaca
     1201 actcacatat cggcgatgct cgcgcgaccg aaatgggcca ggtgcggggg gagcacaatc
     1261 tcggtcagct cgcgcgcgag cgatggggcg atgaggtggc gctggttgga ttcggtaccg
     1321 gatcgggctt tgtcgccgca gcgtcccatt gggatggcga gctagagatc aagcgcatac
     1381 ggcctccgcg atccggctct tatgaagcct gctgccatga cacgggcttg ccttcattcg
     1441 ttttgagcct gctgccggac caaagacgcg aagtcatcga gatcctttcg gagccgcggc
     1501 tggagcgggc gatcggcgtg atttaccgtc ctgagacgga gctcatctca cactactata
     1561 gggcagtctt gccgcggcag ttcgatgttt gggtctggtt caatgaaact ggtcctgtga
     1621 cggccattcc tgtgcctggc gtcacggacg agcgtgagac gtatccgttc ggcctttaga
     1681 gctggactgg attaagaggc agcatttttt ttcagttgtc ggagttattc cgggcggcgg
     1741 aaatccgcca tcagtcggga aacaccagcg aacttcgccc ccgcgttatc gggagcgatt
     1801 cgccagcttg gcggtgacgg agaaaatcag gggcaggaac ggcaagataa tgaagatgcg
     1861 aacgatgtga aacgcggtca cgatcgcgac ctgctggttc aagatcttgg ccgtcagcgc
     1921 catctccgta acgctgcccg gggcagtcgc cagtatcatc gtgtgccaat caatgccggt
     1981 cacagcggcg atggcgaatg ccatgccggc gcataggacg atcagcactc cggtcgagac
     2041 gatggcggcg aggacgaagg cccgcgtccg tgcaagcgtc tcccgatcga acatacaacc
     2101 aagccacacc cccagcagga tctgagcacc ggcaagaacc cagtagggag gcattgaaac
     2161 aggcaaggca aaggctgttg ccgcagcgtt gaagccgaga gctccgagga aaaacgggct
     2221 ggtaatacgc aaccaacgga aaagtagccc gcccgctacg gcaagcgcga acagcagcag
     2281 catccccgga aggcttctct cgccgacggt aagagcggcg ttgggcgctc cgccgctgat
     2341 cgcaaccagc acaggcggaa tgatgagcac caggagtgag atgcgcaggg tttgggccaa
     2401 agcgatgggt gcagggggca cattgtgatg ctcggcgagc gccgccatct ccactggccc
     2461 gccgggtatg ctcgccaggc cagcggcgac gacatccatg tctgccagcc gcatgaggac
     2521 cgcagccgca atgaagccag ccacgatcgt caggagagcg acgagcacca tcggcaaaac
//
LOCUS       DASUMG010000177         3139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1066929, whole genome shotgun sequence.
ACCESSION   DASUMG010000177 DASUMG010000000
VERSION     DASUMG010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066929"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VFX46_02865"
     CDS             complement(<1..565)
                     /locus_tag="VFX46_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX5599555.1"
                     /translation="MMTRLARRSLRVSPISSAVFGLVMLLLCAPAAMADPGTPSKVSA
                     RYKIYFNGIEIGSFRFESNADSAKYSLAGQAKLSALLGVLKWSGTMRSEGILARGEPA
                     PSDYVFRYKSSSDSGSVAMSFDKKRVAETKLEPPQSPSKSRVPLTEEHLKDVLDPMSA
                     VLAMTTGHLDNPCDRKVAVFDGKHRFDI"
     gene            814..1125
                     /gene="rpmB"
                     /locus_tag="VFX46_02870"
     CDS             814..1125
                     /gene="rpmB"
                     /locus_tag="VFX46_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX5599556.1"
                     /translation="MTRRCELSGKLPMSGQLRSHAENKTKRTFRPNLCTVTLISDVLK
                     RSFRLRVSAHALRTVEHRGGLDAYLLKARDEELSLRCRRLKRQIEKAKAAAGAGEAQA
                     A"
     gene            complement(1191..2261)
                     /locus_tag="VFX46_02875"
     CDS             complement(1191..2261)
                     /locus_tag="VFX46_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEX5599557.1"
                     /translation="MRRASRLRRVGLGVLALALSISVAVLVAERAGAQKPVITDTKPI
                     EIVARPIHGFDRQAIDRQQFGRLKWRGGLVLSSLAEEFGGFSGVVLDDDGRRLFAVSD
                     TGAWLSAELDYEGTRPARVQRAWMGPLRARNGAPLTRNRDRDAEAVTLAEGSLTDGIL
                     LIAFEQLHRIGRFRSTEAGPSAPLHYLELPPEAGKMKSNRGIEAVSVLRGGPLKDSTI
                     VFAERLKDERGHHTGWVWVDGKPQKLHLADIGGFDITDSAALDDGGLIVLERRFNWIE
                     GLKMRLRLLRPEEIAVGAVMRGEVLFEADMGYEIDNMEAVAVHRGPHGEIVVTLLSDD
                     NFNSPFQRTVLLQFTLQKEALN"
     gene            complement(2288..>3139)
                     /locus_tag="VFX46_02880"
     CDS             complement(2288..>3139)
                     /locus_tag="VFX46_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEX5599558.1"
                     /translation="AVSRLANRLQRRLLAQQNRAWDFDLDEGMLDPARLPRVIIDPTH
                     PLSFKRERDTDFRDTVVTLLLDNSGSMRGRPIMVAACCADILARTLERCGVKVEILGF
                     TTRAWKGGQSREQWLAAGKPPNPGRLNDLRHIIYKAADAPWRRAKRNLALMMREGLLK
                     ENIDGEALAWAHNRLLARPEQRRILMMISDGAPVDDSTLSVNSGSYLEQHLRQVIEEI
                     ETRSPVELIAIGIGHDVTRYYRRAVTITDPSELAGAMTDKLVELFEEKGPAAPVRKFP
                     KRHLTLH"
BASE COUNT          624 a          992 c          919 g          604 t
ORIGIN      
        1 caatatcaaa gcgatgcttg ccgtcgaaga cagccacttt gcggtcgcag ggattgtcga
       61 ggtgtccagt cgtcatcgcc aagacggcgc tcatgggatc gaggacgtct ttgagatgtt
      121 cctcggtcaa gggcaccctg ctcttcgaag gcgattgggg cggctcaagc ttcgtctcgg
      181 cgacacgctt cttatcgaag ctcatcgcga ctgagccgga gtccgagctg ctcttatacc
      241 ggaagacgta atcggatggc gccggctccc ctcgtgccaa gataccctcg cttcgcatgg
      301 tgccggacca cttgaggaca cccaggagag cggaaagttt ggcctgccca gccaatgaat
      361 actttgccga gtcggcattg gactcaaacc ggaagctgcc gatctcgatg ccattaaagt
      421 aaatcttgta gcgtgccgag accttgctgg gcgtgcccgg atcggccata gccgccggcg
      481 cgcatagcag tagcatcacc agcccgaata cagcgctgct gatcgggctc acccggagcg
      541 agcgccgggc gagtcgtgtc atcatggtga cgcctccaga ttacgacgct ccaccaatcg
      601 gctacggatt attttggtca attgtatgtc gctgccgata caacaagtgc gtgccgcaca
      661 taggctacat ctcgcgtgct tgacgcttgg cctgatgcat aagtatagag ttccgcgaac
      721 tcccggccgc tctactgacg ggacgttgtc gaccccagaa gcggaaagaa ctctatgcgt
      781 ccccctctag ggtgcgccaa gtgaggattt gtcatgacga gacgctgtga gctgagcggc
      841 aagctgccga tgtcgggcca gctgcgtagc cacgccgaaa ataagaccaa gcgcacgttc
      901 cgcccgaacc tgtgcaccgt gacacttatc agcgacgtgt tgaagcgcag ttttcggctg
      961 agggtgagcg cccatgcgct gcggacggtc gagcaccggg gtgggcttga cgcctatctg
     1021 ttgaaggcac gggacgaaga gctatcactg cgctgccggc gactgaaacg ccagattgag
     1081 aaggccaagg cggcggctgg cgccggggaa gcgcaagccg cgtgaggtcg cattcgtgcg
     1141 gcctccgttt tcaagcagtt cacgcaactg atctgctggc ttctgcactt tcagttgaga
     1201 gcttctttct gaagcgtgaa ctggagcaga acggttcgct ggaacgggga attgaagttg
     1261 tcgtccgaca ataaggtgac gacgatctct ccgtgcggtc cccgatggac ggcgacggct
     1321 tccatattgt cgatctcata acccatatcg gcctcgaaca gcacctcgcc ccgcatgacg
     1381 gctccaactg caatctcctc gggtcggagg agccgaagcc tcatcttgag accttcgatc
     1441 cagttgaagc gccgttcgag aactatgaga ccgccatcgt ccagcgctgc cgagtcggta
     1501 atatcgaagc cgccgatatc cgccaggtga agcttttgcg gcttgccgtc gacccaaacc
     1561 caaccggtgt gatggccacg ctcgtccttc agccgctctg cgaacactat agtcgaatcc
     1621 ttgagtggtc cgcctcgcag aacgctcacc gcttcaatgc cccggtttga cttcattttc
     1681 cccgcctccg gtggcagctc caggtaatgg agtggggcag atgggccggc ttcagtgctt
     1741 cgaaaccgac cgatccgatg aagttgctcg aaggcgatca gcaaaatccc gtccgtcagg
     1801 ctcccctcgg ccaaggtcac cgcctcagca tcccggtctc gattacgcgt cagtggagcg
     1861 ccgtttcgtg cccgtaaggg ccccatccag gcgcgctgaa cacgagctgg ccgcgtcccc
     1921 tcgtagtcga gctccgcgga cagccacgca ccggtatcgg acacggcaaa aaggcgccgc
     1981 ccgtcatcgt cgagcacgac gccggaaaac ccaccgaact cttctgcaag cgagcttaga
     2041 accaggccgc cccgccactt caagcggccg aactgttgtc ggtcgattgc ctgcctatcg
     2101 aaaccatgta tgggccgcgc cacgatctcg atcggcttcg tatccgtgat gacaggcttc
     2161 tgcgccccag ccctctccgc gacaaggacc gcaacggaaa tcgacagcgc taaagcgagc
     2221 actccaagcc caacacggcg caatcgcgac gcccgccgca acttcctctc ctatccggta
     2281 gagtcgatca atgcagagtt aagtgccgct tgggaaactt gcgcactggc gcggctgggc
     2341 ccttctcctc gaacagctcg accaacttgt ccgtcatggc gccggccagc tccgatggat
     2401 cggtgatcgt caccgcgcgc cggtaataac gcgtgacgtc gtgcccgata ccgattgcaa
     2461 tgagctcgac cggtgagcgg gtctcgatct cctcgatgac ctggcgcagg tgctgctcca
     2521 gataagagcc tgagttgacg gatagagtcg agtcatccac aggggcgccg tccgaaatca
     2581 tcatcagaat gcgcctctgc tcaggacgtg cgagcaggcg gttgtgggcc cacgccaacg
     2641 cttcgccgtc gatgttctcc ttgagcaggc cctcgcgcat catgagcgcc aagttgcgct
     2701 tagcccggcg ccacggcgca tctgccgcct tgtagatgat gtggcgcagg tcattgagcc
     2761 gacctggatt gggcggcttc ccggctgcga gccactgttc gcgggactga ccacctttcc
     2821 acgcccgcgt ggtgaaaccg aggatctcga cttttacccc gcagcgctcg agcgtacgcg
     2881 cgagaatatc cgcgcagcag gccgcgacca tgatcggccg cccccgcatg cttcccgagt
     2941 tgtcgagcag gagggtcaca accgtgtcgc ggaaatcggt atctcgctcg cgcttgaacg
     3001 agagcgggtg tgtcggatct atgataaccc gcggcaaccg cgccggatcg agcatcccct
     3061 catccaggtc gaaatcccaa gcccggttct gctgcgcaag aagtcgccgc tgcaagcgat
     3121 tggccagccg cgatacagc
//
LOCUS       DASUMG010000178         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_103577, whole genome shotgun sequence.
ACCESSION   DASUMG010000178 DASUMG010000000
VERSION     DASUMG010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103577"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFX46_02885"
     CDS             <1..734
                     /locus_tag="VFX46_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5599559.1"
                     /translation="FCEKPLGLTAASARRSVQACVDAGVQLGVGHERRFEPAIIALAG
                     MIREGSLGTIMHAEAAFSHDKLIHVPPGDWRTVKATAPAAAMTAMGIHLTDLYISLFG
                     RVKTVQAMVAKRVLDWETGDVVTVQLGFEAGMTASLSAVLYTPHFMRMHVFGSKEWVE
                     IRNDTHPDTPGGIAELVRARTGHPHEVVRYNWTDTVVANLEAFAAAIRGTAPYPITHE
                     EMIHNIELLEAIAKSAEDRSTVHLS"
     gene            839..1303
                     /locus_tag="VFX46_02890"
     CDS             839..1303
                     /locus_tag="VFX46_02890"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5599560.1"
                     /translation="MLHDLVSRLPLLIVAVVLGVILVWQSPINAEAARRLGSPALAGV
                     LSISISLVLVVAFAAVTVRAKPDWSQIASAPWWAWIGGIAGAVFVVGAAVIVPKTGSV
                     LFLLAVVLGQMLGAVVADTYGMWGLPVQPISLAKLAGISLVLAGAVVFILSS"
     gene            complement(1325..1627)
                     /locus_tag="VFX46_02895"
     CDS             complement(1325..1627)
                     /locus_tag="VFX46_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX5599561.1"
                     /translation="MPAYWVARSRIIDPVEYKKYTDRVPEIVAKYGGKILARGGRYEI
                     LEGPEVFHRFVVIEFPSLEQAKACHSSPEYQEASAFRKNGAGEVELVIVEGGDATK"
     gene            complement(1657..3006)
                     /locus_tag="VFX46_02900"
     CDS             complement(1657..3006)
                     /locus_tag="VFX46_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5599562.1"
                     /translation="MFDAFQMTLLVVVLLGLVLFGTHIAVALGLTAALGVYLMMGDME
                     VLTSFLANTAYEAIRDYIFAVIPLFILMGEFLARCGAARDLFALVNRVMRWLPGRLGV
                     ATVVANALFAFVTGVSIAAAAAFSRIAYPEMKRHGYQRRFALATIASSACLGMLIPPS
                     VLMIVWGVITEESIGSLFLAGVIPGLIVVAGFSIYIILVAILNPSIVGEAKARALSAA
                     VPVVEEETGTEPSVLSALLSTGLIICLIVGVLGGIWAGFFTPTEGAGVGAAGALVIGI
                     MKGLRLKDISEVVLTVGRTTAPLLLLLVMAQIYSRVLSMTGVTGAVQAFFLDTGLSPM
                     AIISVMILIWFLLGCVIDSISIILLTVPVFAPIASALGFDPLAFAIIGIIAVETGLLT
                     PPFGILVFTVKASLPHERVPVGEIFAGCIPFWIILLGTVVLLVLVPETATWLPNLGR"
     gene            complement(3019..3534)
                     /locus_tag="VFX46_02905"
     CDS             complement(3019..3534)
                     /locus_tag="VFX46_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEX5599563.1"
                     /translation="MAEKLLGWIARSLLLLASLLAFALCFLVVADVIGRAGFNHPVQG
                     TPEIVSSSIVVICYLMATYAILSGGMMEVTLVTDILPPVPRALLGAVTFLLGALLFGL
                     IGWGSVERFVTDWTSGAYEGEGALRVPTWPVRLSVLVGSVLATLAYILLAIRAVRAAV
                     RGEPFSSSITH"
     gene            complement(3612..>4306)
                     /gene="dctP"
                     /locus_tag="VFX46_02910"
     CDS             complement(3612..>4306)
                     /gene="dctP"
                     /locus_tag="VFX46_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX5599564.1"
                     /translation="LARAVYDRVPLLSKIFEDKYNQKLLALIADNGYNLTTNFEWKTV
                     ADLKGQKIAGAGLNLKWLEYAGVVPVQSSLVEAYTSLQTGVYNGWIMFPSGVMNFKLY
                     EVSKYYTEIGFGAITWHGLTINLRRFERLPKEVQDILLEVGREYEELTGTVNEENYPK
                     QIEELKKVGAVVNQIDESVRIEWANSLKDWPREKAKELDAAGLPGTEVLKAALEEAEK
                     LGHKWPVRYEID"
BASE COUNT          910 a         1332 c         1280 g          784 t
ORIGIN      
        1 ttttctgcga gaagccgctt ggtctcacgg ccgccagcgc gcgccggtct gtgcaagcct
       61 gcgttgatgc cggagtgcag ctcggtgtcg gtcacgaacg gcgtttcgaa cctgccatca
      121 tcgcgctagc cggcatgatc cgggagggct cgctcggcac catcatgcat gccgaagctg
      181 ccttcagcca cgacaagctc atccacgtgc cgcctggcga ctggcggaca gtcaaggcta
      241 ctgcaccagc cgccgcgatg acggccatgg gcatccacct gaccgaccta tacatttcgc
      301 tattcggccg ggtgaaaacg gtgcaggcga tggtggccaa acgcgtgctc gattgggaga
      361 cgggcgacgt cgtcacggtg caactcggtt tcgaggcagg catgacggcg tctttgagcg
      421 cggttctcta cacaccccac ttcatgcgca tgcacgtgtt cggctcgaag gaatgggttg
      481 aaatccgcaa tgacacccac ccggacacgc caggcggcat cgcagagctc gtacgtgcac
      541 ggacgggaca tccgcatgag gttgtccgct acaattggac agacaccgtg gtggcgaatt
      601 tggaagcctt cgcggccgct attcgtggaa cggctcccta tcccatcacg cacgaggaaa
      661 tgattcacaa catcgagctg ctggaggcca tcgccaaatc ggctgaggac cgctcgacag
      721 ttcacctaag ctgagcaata actctggaac cctccaactt gcgcggggcg cctcgatcga
      781 gcgggattgg ggtgtctcgc gtttgtgccc aattttcccc gcaaaggatc gttcctcggt
      841 gcttcacgac ctcgtcagtc gactgccgtt gctcatcgtt gctgttgtgc tcggcgttat
      901 tctggtttgg caaagtccca tcaatgcgga agcggcccgg aggctcggct caccggcgct
      961 cgcgggcgtg ctgtcaatca gcatcagtct ggtgctggtc gtggcatttg cggcggtaac
     1021 cgtccgcgcc aagccggatt ggtcccagat tgctagcgct ccctggtggg catggatcgg
     1081 cggcatcgcc ggcgccgtgt tcgttgtcgg cgccgcagtg atcgtgccga agacgggcag
     1141 cgtgctattc ctgcttgccg ttgtgctcgg ccagatgctc ggcgccgtcg tagctgacac
     1201 ctacggcatg tggggcttgc ccgtgcagcc gatatcgctg gccaagctcg caggtatcag
     1261 cctcgtcctc gctggcgcgg tcgtctttat cctcagcagt tgaaacgtat cacgggcgca
     1321 cgatttactt cgttgcgtcg ccgccctcga cgatcaccag ctccacctca cccgcaccgt
     1381 tcttccgaaa agcggaggct tcctgatact cgggcgaaga atggcatgcc ttggcctgct
     1441 ccagtgacgg gaactcaatg acgacgaacc ggtgaaaaac ttccgggccc tcgagaattt
     1501 cgtaacggcc gccgcgcgcc agaatttttc cgccatactt ggcgacgatc tcaggaacac
     1561 ggtccgtgta cttcttgtac tcgacggggt cgatgatgcg cgagcgggca acccagtaag
     1621 caggcatggt tcgtccttct tctcctttgg ttgattttaa cgtccaagat tggggagcca
     1681 ggtagccgtt tccggcacca aaacgagaag cacgaccgtg ccgagcagaa tgatccagaa
     1741 cgggatgcaa cccgcaaaga tctccccgac cggaacacgc tcatgcggca gcgacgcctt
     1801 caccgtgaac acgagaatgc cgaacggcgg cgtcaggagg cccgtttcga cggcgatgat
     1861 tccgatgatg gcgaaggcta acggatcgaa gcccaaggca ctcgcgatcg gcgcgaagac
     1921 gggcaccgtc agcaggatga tcgaaatgga gtcgatcaca cagccaagta agaaccagat
     1981 caggatcatc acgctgatga tggccatcgg ggacaggccg gtgtcgagga agaacgcctg
     2041 tacggcaccc gtcacgcccg tcatggagag cacgcgcgag tagatctgcg ccatcaccaa
     2101 cagcagaagg aggggcgccg tggtgcgccc tacggtcagc accacctccg aaatatcctt
     2161 cagccgcagg cccttcatga ttccgataac aagcgcgccc gcggcgccaa cgccggcgcc
     2221 ttcggtcggc gtgaagaaac cggcccaaat gccgccaagc acgccgacga tgaggcagat
     2281 gatgaggccc gtgctgagca gcgccgaaag aactgaaggc tcggtccccg tctcctcctc
     2341 gacgacagga actgcagcag agagcgcgcg cgccttggcc tcgccgacga tggacggatt
     2401 caggatcgcg acgagaatga tgtagatgct gaagccggca acaacgatca gaccagggat
     2461 cacgccggcg aggaacaggc tgccgatcga ctcctctgtg atgacgcccc aaacaatcat
     2521 cagcaccgag ggcgggatca gcatgccgag acacgcactc gaggcgatgg tggcgagcgc
     2581 gaaacggcgt tgatagccgt gccgcttcat ctccggataa gcgatgcgcg agaacgctgc
     2641 cgccgcggcg atggacacgc cggtgacgaa ggcgaacaaa gcattcgcga caacggtcgc
     2701 gacgccgagc cggcccggga gccaacgcat taccctgttg acgagagcga agaggtcgcg
     2761 ggcggcaccg cagcgggcga ggaactcacc catcaaaatg aaaagaggga tgacggcgaa
     2821 aatgtaatcg cgaatggctt cgtaggcggt gttggcgagg aagctcgtca acacctccat
     2881 atcgcccatc atcaagtaaa cgccgagcgc tgccgtcagc ccgagcgcca ctgcgatgtg
     2941 ggttccgaag aggaccaggc ccagcaggac cacgacgagc agggtcatct gaaaagcgtc
     3001 gaacattcga acacctgctc aatgcgttat gctggaggag aacggttcgc ctcggacagc
     3061 tgcccggact gcgcggatcg ccagaagaat gtaggccagc gtcgccagca cacttccgac
     3121 gagtactgag agccgcactg gccacgtcgg cacccgcaag gctccttcgc cctcataagc
     3181 tccgctggtc cagtccgtca cgaagcgctc gaccgagccc caaccgataa gtccaaatag
     3241 gagcgccccc agcaaaaagg tcacggcgcc gagcagcgcg cgaggtacgg gagggagaat
     3301 gtccgtcacc aaagtgacct ccatcatccc gccgctgagg atggcgtaag tcgccatcaa
     3361 ataacaaatg acgacgatcg agctggaaac gatttctggc gtcccctgaa cggggtgatt
     3421 gaaacccgct cggccaatga cgtcggccac aacgagaaag cacaaggcaa aggcgaggag
     3481 cgaagccaac agcagcaggc ttctggctat ccacccgaga agcttttcgg ccatccaatt
     3541 caccatcagt gccgggcggc tctcacccgg agccttgctg cggctccggg tgctcgagat
     3601 cctgaatcag ttcagtcgat ttcgtagcgc accggccact tgtggccaag tttctcggcc
     3661 tcttccaggg cagctttcaa gacttcagtc cccggcaggc cagccgcatc gagctctttt
     3721 gctttctcac gcggccaatc cttgagcgag ttcgcccatt cgatgcgaac ggattcgtcg
     3781 atctggttga cgacagcgcc aaccttcttc agctcctcga tctgcttcgg ataattctcc
     3841 tcgttaaccg tgcccgtcag ctcctcgtac tcccggccga cctccaggag gatgtcctga
     3901 acctctttgg gcaggcgctc gaaccggcgc aagttgatcg tgagcccgtg ccaggtgatc
     3961 gccccgaagc cgatctccgt gtagtacttc gagacctcgt acagcttgaa gttcatgacc
     4021 ccggacggga acatgatcca gccgttgtag acccctgttt gcagcgaggt gtaggcttca
     4081 accagcgagg actgcactgg cacaacgccg gcatactcca gccacttcaa attgagaccg
     4141 gcaccagcga ttttctgccc tttgagatcg gctacggttt tccactcgaa gtttgtcgtc
     4201 aggttgtagc cattgtccgc aatcagggcg agcagcttct ggttgtactt atcctcgaaa
     4261 atcttgctga gcagaggaac ccgatcatag acggcgcggg cgagct
//
LOCUS       DASUMG010000179         4080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1067111, whole genome shotgun sequence.
ACCESSION   DASUMG010000179 DASUMG010000000
VERSION     DASUMG010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067111"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..748)
                     /locus_tag="VFX46_02915"
     CDS             complement(71..748)
                     /locus_tag="VFX46_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599565.1"
                     /translation="MIVIVDERQTVSEGFASWFDREGVSATGLRPAEFDQWVDSVSQP
                     DIMAVEAFLLGDCRERFALSRKIKGKAATAPVIAMNERRSLEETLELFAAGVDDVVRK
                     PIHVREILARIEAIRRRGNQSGPECAEAGAIRVFFDGRDPEVHGEVLPLPRRERRILE
                     YLVMNKGRRVTKTQIFNSVYGLFGENIDENVIESHISKLRKRLRQRLGHDPIDSQRYL
                     GYRLVFQ"
     gene            complement(1115..2323)
                     /locus_tag="VFX46_02920"
     CDS             complement(1115..2323)
                     /locus_tag="VFX46_02920"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX5599566.1"
                     /translation="MAQRDKVSNFSQQWAPETILVHGGQLRTEFGETAEALFLTQSYV
                     YDSAEQAEARFAGNEPGFIYTRFSNPTVAMFEERMRLLEGAEAARATATGMAAVTAAL
                     LCYLQTGDHIIAARAMFGSCRYIVDELCPRFGIETTLVDGSDIEQWRKALRPNTKALF
                     FETPANPTLEVVDIAAVSALAHDVGALVFVDNVFASPILQKPLALGADVVMYSATKHI
                     DGQGRCLGGVVLCSEKFLKDYLHTFLRQTGPSLSPFNAWVLLKGLETLSLRVKAQCAT
                     AAKIADWLAEQSLVERVRYCGRPDHPQADIIRRQMSGGGQVVTFEIAGGKERAFRFVN
                     ALKLIRISNNLGDAKSLITHPATTTHQRLSPEARAELGITDSMLRLSVGLEAAEDLIA
                     DLEAGLAAAA"
     regulatory      complement(2399..2476)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            2630..3733
                     /locus_tag="VFX46_02925"
     CDS             2630..3733
                     /locus_tag="VFX46_02925"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX5599567.1"
                     /translation="MGDQAASEKANGILPAQAIRQLIADGHIQLAEPMEDKQLQPASL
                     DLRLGDCAWRVRASFLPGPKARVEEKLADLALHKIDLTQSAVLETGCVYVAPLLERLA
                     LPPDISAAANPKSSIGRIDVFTRVIADAVASFDQVPAGYSGPLYAEICPQTFPIVVRR
                     GSRLSQVRFRQGTFADSDAALADLHRRERLVSSDNPDISQGIALSVDLVGDENGLVGY
                     RAKRHTGLIDVDATGKYDILDYWEPIKLHGEKRLILDPYEFYILASREAVNVPPTHAA
                     EMVPFNPLVGEFRVHYAGFFDPGFGYAASGGAGSRAVLEVRSHKVPFILEHGQIIGRL
                     IYERMAEVPEMLYGAALGSHYQAQGLKLSRHFK"
     gene            complement(3746..>4080)
                     /locus_tag="VFX46_02930"
     CDS             complement(3746..>4080)
                     /locus_tag="VFX46_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5599568.1"
                     /translation="PFVHPPIEDVALESVLYALADPVRLAILLKLASGNCPMNCSTAA
                     PEKLPKSTQSHHYKVLREAGLIRSERRGTEVVNTLRCDEINAKFPGVLPAILKAAAAQ
                     FGQATQKP"
BASE COUNT          817 a         1269 c         1225 g          769 t
ORIGIN      
        1 cccggcttgc acaaaactga tggggagcga tcgcgccgca cgaccgctcc ccgcgcgcta
       61 ccccgctgcg ctattggaaa acgagccggt acccgagata gcgctgtgaa tcgatcggat
      121 catgaccaag ccgctgacga agccgcttgc gcagcttgct gatgtggctc tcgatcacgt
      181 tctcgtcgat gttctcgccg aacaaaccgt acactgagtt gaagatctgg gtcttcgtaa
      241 cgcgccggcc cttgttcatg accagatact caaggatgcg ccgctcccgc cgtggcaatg
      301 gcagaacctc gccgtgcacc tcgggatcgc gaccatcgaa gaagacacga atcgcgcccg
      361 cttctgcgca ttccgggccg ctctgattgc cacggcggcg gatcgcctcg atcctcgcca
      421 ggatttcgcg tacgtggata ggcttgcgaa cgacgtcgtc cacaccggcc gcgaagagct
      481 cgagcgtctc ctcaagagac cgccgctcgt tcatagcaat gacgggcgct gtggcagcct
      541 ttcccttgat cttgcgagaa agtgcaaagc gttcgcggca atccccgagg aggaacgctt
      601 cgacggccat gatgtcaggc tgagatacgc tatcgaccca ctgatcgaac tccgctggtc
      661 gtagaccggt tgccgagacg ccctcccggt cgaaccaaga cgcgaaccct tccgacacag
      721 tttggcgctc gtcgacaatc acgatcattg ctgcccccct agtcagattt gatcgtctgc
      781 actttttgcg tataaccctt tgttagatcc gtcaatatgc gcagactacc caccaaacgt
      841 gaagatttgc ttattcttgg ggagcgtgat cgttcgccca caccggggac agcggcctta
      901 taacggggtg attttggagc tagcagaaga gaggagaggg ccaccgcgga ccggccagaa
      961 ttcctcagcc aagcgaactt tcgctgcgcc aaagcgaact tcctccgcgg aggcctttgg
     1021 cgcgcacgcg gcttctgtcc gcggcacaga actcccgaaa gccgcgaact aacgcggttt
     1081 gctgatggtg atgagccgtg aagggcggac gctattacgc cgccgcggca agccctgctt
     1141 cgaggtcagc aataagatcc tcggccgcct cgagcccgac cgacaaccgc agcatgctat
     1201 ccgtaatgcc gagctctgcc cgtgcctcgg ggctaaggcg ctggtgggtc gtcgtcgccg
     1261 gatgagttat gaggctcttc gcgtctccga gattgttgga gattcggatg agcttcaggg
     1321 cattaacgaa tcggaaagcg cgttcttttc cgccggcgat ctcgaaggtc accacctgac
     1381 cgccgcccga catctgccgc cggatgatgt cggcctgtgg gtgatccggg cggccacaat
     1441 agcgcacccg ctcgacgagg gattgttccg ccagccaatc ggcgatcttt gcggccgtgg
     1501 cgcactgtgc tttcacgcga agcgacagcg tctccaatcc tttcagcaac acccaggcat
     1561 tgaatgggct gagcgaaggc ccagtctggc gcaggaatgt gtgcaggtaa tcttttagaa
     1621 acttctccga gcacagcacg acgccgccga gacaccgtcc ctggccatcg atgtgcttcg
     1681 tcgccgaata catgaccaca tcggcgccca acgcgagcgg cttctggaga attggcgagg
     1741 caaaaacgtt atcgacgaaa acgagtgcgc caacgtcgtg cgcgagcgcc gaaaccgctg
     1801 cgatgtcgac cacctccaga gtcggattgg ccggtgtttc gaaaaaaagc gctttcgtgt
     1861 tgggccgcag ggctttccgc cattgttcga tgtcgctgcc gtcaacgagg gtcgtctcga
     1921 tgccgaagcg aggacaaagc tcatcaacga tatagcggca ggagccgaac atcgcgcgcg
     1981 ccgcgatgat gtgatcgccg gtctgaagat agcacagcag ggccgcagtc accgccgcca
     2041 tgcccgtcgc cgtcgcgcgt gccgcctcgg cgccttccag caggcgcata cgctcttcga
     2101 acatcgcgac ggtcggattg gaaaagcgcg tatagatgaa accgggctcg ttgccagcaa
     2161 aacgagcctc cgcctgctca gcagagtcgt agacgtaact ttgggtgaga aagagcgcct
     2221 cggcagtctc gccaaactcg gtccgtagtt gaccgccgtg gacgagaatc gtttcgggcg
     2281 cccattgctg gctgaaattc gaaactttat cgcgctgtgc catggacgcc tccttacctg
     2341 tctggcccag ggcaaaaaga aaacccggcc gcagatctcg caagaaaccg agcgcgggcc
     2401 aggtttgcgc gcggcctttt agcgagttct ttaacgtggc tgcaagccgg ccggccaaat
     2461 caccacggtg cggttgcgct gatacgcctg atgcgcgcga tcgtcaaggt cgaaggccaa
     2521 tcaattattc ggcaggctgc tgtgcgagct tgcaagacgt ggagagacgt aagcgcgact
     2581 tggcgcgaaa gggctgaaca gctagacacg tctcctacgg aggtgcggca tgggcgatca
     2641 agcggcttca gaaaaagcga acggaatttt gcccgcccag gcgatccggc agctgatcgc
     2701 cgacgggcat atccagctcg cagagccgat ggaggacaag cagcttcagc cagcgagtct
     2761 tgatcttagg ctcggtgact gcgcctggcg cgttcgcgcg agcttcctgc cgggccccaa
     2821 agcgcgagtc gaggagaagc tcgccgacct ggcattgcac aagatcgacc tgacgcaatc
     2881 cgcagtcctc gaaacgggat gcgtctacgt cgcgccgctc ttggaacggc tcgccctgcc
     2941 tcccgatatc tccgcagcgg ccaacccgaa gagctcgatc ggccgaattg acgttttcac
     3001 gcgagtcatt gccgatgcgg ttgcctcgtt cgatcaagtg cctgccggat actctggtcc
     3061 cctctatgcc gagatctgcc ctcaaacctt ccctatcgtc gtaaggcgcg gctcgcgcct
     3121 gagccaggtg cggtttcgcc aggggacgtt cgcggacagc gacgccgcgc tcgccgacct
     3181 ccaccggcgc gaaaggctcg tatcctccga caatccggac atctcgcagg ggatcgcgct
     3241 gtcggtcgac ttggtgggcg atgagaacgg actggttggc taccgcgcga agcgccacac
     3301 cgggctcata gacgtggatg cgaccggcaa gtacgacatc ctcgactact gggagcccat
     3361 caagctgcac ggcgaaaagc ggctcattct cgatccttat gagttctaca tcctcgcctc
     3421 gcgggaggcc gtgaacgttc caccgacgca cgcagcggaa atggtgccct tcaatccgct
     3481 cgtcggcgag tttcgggtgc actatgcagg tttcttcgac cccggatttg gctatgcggc
     3541 ctcaggcggc gcgggctcgc gcgcagtcct cgaagtgcgc tcgcacaagg tgccgttcat
     3601 actggagcac ggccagatca tcggtcggct gatctacgaa cgcatggcgg aggtgccaga
     3661 gatgctttac ggcgcggccc tcggcagcca ttaccaggcg caagggctga agctctcgag
     3721 gcacttcaag tgagggaaag aacgctcacg gtttttgcgt cgcctggcca aattgcgccg
     3781 cggctgcttt cagaatggcg ggcaaaacac cgggaaactt cgcattgatt tcgtcgcaac
     3841 ggagcgtgtt gacgacctcc gtcccccggc gttccgagcg gatgagaccc gcttcgcgca
     3901 acaccttgta gtggtgcgat tgagtcgatt tcggcaattt ctccggcgcc gccgtcgaac
     3961 agttcatcgg gcagttgccc gacgcgagct tcagcaggat cgcaaggcgc acaggatcgg
     4021 caagcgcgta gagaacgctc tccagcgcca catcctcgat cggcggatgc acaaagggcc
//
LOCUS       DASUMG010000180         2686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_345290, whole genome shotgun sequence.
ACCESSION   DASUMG010000180 DASUMG010000000
VERSION     DASUMG010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345290"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..1911)
                     /locus_tag="VFX46_02935"
     CDS             complement(133..1911)
                     /locus_tag="VFX46_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5599569.1"
                     /translation="MHLRLIPHLIVFAVSALLLGLGADNAFARQGVLLPAVCDHRDGR
                     LIVQASQPTAHEIVSELKRQVFSACRPGRSDRCRPMMVYQFEVACGAVHVSWMDLATA
                     LLSAGGTSAWIEGERLHIRPRSTRPRLGDCDNGDPTNGFLRKCEPWELDGLREEWVLP
                     PGFAPVHEVGARFVDLPPPAEPPLPEPEVAAFDNEELQAPSDSEAVSTGASSIGAPAD
                     STETLWASDNDTPAVAQEDIAHPHPQSDAEVETAYEPFDATQSPSEGNGSTASIEHTD
                     THSFVRNVSEAIAAAASAEPETVVSTAEAEPASSVRSWSAMLEFASSKTFTWLSESFL
                     SAFDPLAAALKDPRLEKEVTAALMGIALLSCLVSGIGWYSVRARRARAPAFRAADGRQ
                     PVVHTFRPPSRSEAESRDAAGQASASTALVAPTDEKICGELCRTAHTLLQQIEVRVDD
                     LQGVAPLRRVLQREMRGLEQFLTAVMTASPQEPEEWRRMRNRLQRIVRELHRLRDIVE
                     GAYRSLSTGGFTSREPPRDKYEAYEALGVNPDVDLRTLKKLVDALRACWHPDLAKDET
                     DRANREERMKRINIAWDIITSKRQEA"
     gene            2309..>2686
                     /locus_tag="VFX46_02940"
     CDS             2309..>2686
                     /locus_tag="VFX46_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX5599570.1"
                     /translation="MPSYKIAMAASDQDMARTLSGALSDLVEPPPDAVTTFESDDGWT
                     VEAYYNEHPDSERLTKELGALLSFAIPQIDVVEVPDENWVAISQAALPPVTAGRFTVH
                     GSHDRGLVPRGPNTLLIDAGEAFG"
BASE COUNT          496 a          862 c          824 g          504 t
ORIGIN      
        1 tgagacccgt tcgtcggacc aaggcgcaac aggccggacg acgtccggcc tgtatgtttt
       61 tgggggcgct agattggatc tgacccgtcg aactcctcct tcgatgcggg ccgtccggac
      121 ggtcgcagga ggtcacgcct cctggcgctt cgaagtgata atgtcccagg cgatgttgat
      181 gcgtttcatg cgctcctcac ggttcgcccg gtcggtctcg tccttcgcca aatccgggtg
      241 ccagcaggcg cgcagcgcat ccacgagctt cttcagcgtc cgtaagtcga catccggatt
      301 aacgccaagc gcctcgtagg cctcgtactt gtccctcggc ggctcgcggc tcgtgaaccc
      361 gcccgtagac aggctgcggt aggctccttc gacaatgtcg cgaagcctgt gcagctcccg
      421 cacaattcgc tgcaggcggt tgcgcatccg ccgccattcc tcaggctcct gaggcgaggc
      481 ggtcatcacc gcggtcagga actgctccaa gccgcgcatt tcgcgctgaa ggacgcgacg
      541 gaggggcgcc acgccctgca agtcatccac ccgaacctcg atctgttgca acagggtgtg
      601 tgcagtgcga cacagctctc cgcagatctt ctcgtccgtt ggagcgacaa gcgccgtgct
      661 ggccgatgct tggcccgcgg cgtcgcgcga ttcagcctcg cttcggctgg gtggccggaa
      721 ggtgtggaca acaggctgcc tcccatcggc tgccctgaag gcgggcgccc tggcgcgtcg
      781 ggcccttacg gagtaccagc cgattccgga aaccaggcac gacaagagcg caatgcccat
      841 cagtgccgcc gtgacctcct tctcgagtcg cgggtctttg agagccgcgg ccagaggatc
      901 gaatgcgctc agaaaggact ccgacaacca cgtaaaggtt ttgctgctcg cgaactccag
      961 catcgccgac cagctgcgga cggaactggc gggttccgct tcggctgtgc tgacaactgt
     1021 ttcgggttcg gccgacgcag cggctgcgat agcctccgag acgttacgta cgaaagagtg
     1081 agtgtcggtg tgctcgatgc tggctgtgct tccgttcccc tccgatgggg actgggtagc
     1141 gtcgaacggc tcgtaggccg tctcgacctc cgcatcggac tgtgggtgtg ggtgggcgat
     1201 gtcttcctgg gcaacagcag gcgtatcgtt atcgctcgcc cagagtgttt ccgtgctgtc
     1261 agcaggggcg ccaattgaag aggcgccagt tgagaccgct tctgagtcac ttggggcctg
     1321 cagctcctcg ttgtcgaaag ctgctacctc cggctctggc aacgggggct cggcgggcgg
     1381 aggcaagtca acgaaccggg ctccgacctc atggactggc gcgaagccgg gcggcagcac
     1441 ccattcctct ctcagcccat ccagctccca aggttcgcac ttccgcaaga acccattcgt
     1501 cggatcgcca ttgtcgcaat ccccaaggcg gggccgtgtc gaccgcggtc tgatgtggag
     1561 acgttcaccc tcgatccacg ccgaggtccc gccggcggac aggagggccg tcgcgaggtc
     1621 catccacgac acgtgcacag cgccgcaggc cacctcgaac tgatagacca tcatcggccg
     1681 gcaacggtcg gaacggccag gccggcaagc cgagaacact tggcgcttca actccgagac
     1741 gatctcatgc gcggtcggct gcgaagcctg tacgatgagg cggccgtcac gatgatcgca
     1801 gacggcgggc agcaagacgc cttggcgcgc gaaagcattg tccgcaccaa gtccgagcag
     1861 caacgcggac accgcgaata caataaggtg cggaatcaag cgtagatgca cctagcagcc
     1921 cccatgcagg cacgacagca tgccgcatat ctggcaagag cggcatgact gtgacagccc
     1981 cgtcatttta tcgggttgtc tagccgctgc aggcgaattc ttttgccctt gcttgataga
     2041 accttaatca catcgcccgc acatcgcaga cttcagaggc taaattcttg aaatacttga
     2101 ggctttcggt tcaatgcggg gcaggcagat gagccggatg cgtcacaatc aacatcgagc
     2161 gtctattggc gcaggccacg cgatgacatc agcaaccttg ggcaagaaca gctcggcctg
     2221 acggtttgcc caatcgacag agtagctgtg acgcgctacc tcgccgcccg aagccagttg
     2281 cgtctcagtc accggatctc gttcaccgat gccctcttac aagatcgcca tggccgcgag
     2341 cgaccaggac atggcccgta cactgtccgg tgcactgtct gatctggttg agccgcctcc
     2401 cgatgcggtc accacgttcg agtcggacga tggatggacc gtcgaggctt actacaacga
     2461 gcatcctgac agcgagcggc tcaccaaaga gctcggcgcc ctgctgtcgt ttgccatacc
     2521 ccagatcgac gtggtggagg tccctgacga gaactgggtg gccatctccc aagccgccct
     2581 gccgccagtg accgcgggac ggttcacggt ccatggcagc catgatcgcg ggctcgttcc
     2641 tcgaggccct aacactttgc tgattgatgc cggcgaagct ttcggc
//
LOCUS       DASUMG010000181         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_746549, whole genome shotgun sequence.
ACCESSION   DASUMG010000181 DASUMG010000000
VERSION     DASUMG010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746549"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1002)
                     /locus_tag="VFX46_02945"
     CDS             complement(118..1002)
                     /locus_tag="VFX46_02945"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599571.1"
                     /translation="MAKVAWIGLGVMGYPMAGHILKKGGHELVVYNRTAAKAEAWVKE
                     YGAGRAASTPAEAAADADFVFSCVGNDDDLRSVTIGENGAFQTMKKGSIFIDNTTASA
                     NVARELYAAAKERGFDFLDAPVSGGQSGAQNGVLTVMVGGDPDVFERAKPVISNYARM
                     VTLIGPAGSGQLTKMVNQICIAGLVQGLAEGIHFAQKAGLDIRLVMETISKGAAGSWQ
                     MENRWQTMAEGKFDFGFAVDWMRKDLMISLDEARRNGASLPVAALVDQFYSEIQRMGG
                     GRWDTSSLIARLNNADKK"
     gene            complement(1159..1572)
                     /locus_tag="VFX46_02950"
     CDS             complement(1159..1572)
                     /locus_tag="VFX46_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599572.1"
                     /translation="MMKSLTIATAALALGGTALFASMPAEAAANAKGSGVIIEARLGG
                     PGPAHVKNRKPSRVAATGVARGALLPYFLKKAQAQRKAINNWSAKVRLMYGSQYASWS
                     RANAKSTSCNRLGSTVTCRVSAVPHSPYRRFGMLN"
     gene            1817..>2090
                     /locus_tag="VFX46_02955"
     CDS             1817..>2090
                     /locus_tag="VFX46_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX5599573.1"
                     /translation="MQGEAKEPRESIAAPAGAVSLADIEKAARVLEGSIVATDCDQSH
                     TLSTMFGCEIWLKFENLQFTGSFKDRGALNRLSALSQEERAAGVIAM"
BASE COUNT          400 a          640 c          650 g          400 t
ORIGIN      
        1 ctagggcgcc ggtgccccga agaagtcgga gtcaagttgc gcgtgggaaa tgcttctcag
       61 ggttgcaaat caggcggtgc ctcgcctaag cgctgcaccg cctgaaaaga cttccgccta
      121 cttcttgtcg gcgttgttga gcctggcgat gaggctcgac gtgtcccaac ggccgccgcc
      181 catcctctga atctcggagt agaactgatc gacgagcgcg gccaccggca agctggcccc
      241 gttccggcgg gcctcgtcca ggctgatcat caaatcctta cgcatccaat cgacggcgaa
      301 gccgaaatcg aacttgccct cggccatggt ttgccaacgg ttctccatct gccaggagcc
      361 ggccgcgccc ttcgaaatcg tctccatcac gagccggatg tcgagcccgg ccttctgggc
      421 gaagtgaatc ccctccgcga gtccttgcac gagcccggcg atgcagatct ggttcaccat
      481 cttggtgagc tgacccgagc cggcggggcc gatgagggtc accatgcgcg catagttgga
      541 gatcaccggc ttcgcgcgtt cgaaaacatc cgggtcgccg ccgaccatga cggtcaacac
      601 gccattctgg gcgccagatt ggccgccgga aacgggcgca tcaaggaagt cgaaacccct
      661 ctccttggcc gcagcataaa gttcccgggc gacgttggca gaggctgtcg tgttgtcgat
      721 gaagatggag cccttcttca tggtttgaaa ggcgccattc tcgccaatgg tgaccgagcg
      781 taggtcatca tcgttgccga cgcaggagaa cacgaagtcg gcatcggcgg ccgcctcggc
      841 cggtgtgctc gcagcccggc cggcgccgta ctccttcacc cacgcttcgg ccttcgctgc
      901 agtccggttg tagacgacga gctcatgacc gcccttcttc aggatgtgtc cggccatggg
      961 atagcccatt acgccgagcc cgatccatgc aacctttgcc atttcgcctc ctccatgggt
     1021 tcttctggtg ggattgagcg agggttctag cccatgctga ggataatgtc aggtccggga
     1081 ggtgcgaaac aggtcagcga aaggaaaagg gagcctgaag gctccctgtc aggtgtacgc
     1141 aatcactttg ctcttcgttc agttcagcat gccgaagcgc cgatagggcg agtgggggac
     1201 ggcgctgaca cggcaggtca ctgtcgaacc gaggcgattg cagctcgttg actttgcgtt
     1261 ggcacgggac cagctcgcat attgagagcc atacatcaga cgcaccttgg cgctccagtt
     1321 gttgatggct ttccgctgcg cctgcgcctt cttcaagaaa tacgggagca gagcaccccg
     1381 tgcgacaccc gtggcagcga cgcggctcgg cttacgattc ttgacgtgcg cgggtccggg
     1441 tccacccaaa cgcgcctcga tgatcacgcc cgagcctttc gcattggcgg ccgcctcagc
     1501 cggcatcgag gcaaagagag ccgttccgcc gagcgcgaga gctgctgtag cgattgttag
     1561 cgacttcatc atgatcaatc tccatcctgt tgcttgcctc gagccgcgca ctgtgcgcgt
     1621 gccccattga cgaggccgaa gttagagtcg accggctgag gtgatgctga acgggttgtt
     1681 taaccagcgt tcattgccgc ggcccgtttc gcgagatggc gcggggtgct aagcagcagc
     1741 ctgaagacgc gttcgaagcg gcgaccagtg acccgcgaac tgccgacggc aggatgcacc
     1801 aaggacaacg aaagcgatgc agggcgaagc aaaggagccc cgagagagca ttgccgcacc
     1861 agcaggagcg gtgagcctcg ctgacatcga gaaggctgcg cgggtgctcg aaggctcaat
     1921 tgtcgcgacc gattgcgatc agagccatac gctttcgacg atgttcgggt gcgagatctg
     1981 gctgaagttc gagaacctgc agttcacggg ctccttcaag gatcgcggcg cgctaaaccg
     2041 cctcagtgcc ctctctcagg aggagcgagc ggcaggcgtc atcgccatgt
//
LOCUS       DASUMG010000182         5784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_907455, whole genome shotgun sequence.
ACCESSION   DASUMG010000182 DASUMG010000000
VERSION     DASUMG010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907455"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3056)
                     /gene="smc"
                     /locus_tag="VFX46_02960"
     CDS             complement(<1..3056)
                     /gene="smc"
                     /locus_tag="VFX46_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX5599574.1"
                     /translation="MHIQRLRLLGFKSFVEPTDLLIEKGLTGIVGPNGCGKSNLLEAL
                     RWVMGETSYKSMRASAMEDVIFSGTTSRPPRNSAEVTLIIDNRDRKAPAEFNDSDIIE
                     VTRRIERDAGSTYRINGREVRARDVKILFEDAATGARSPALVRQGQIGEIVNAKPEQR
                     RRILEDAAGIAGLHSRRHEAELRLNAAEANLARISDILGQLNSQLENLKRQARQVKRY
                     KEISAEIRRAEALLLYLQWKEAQAQVEAEEAALTEVTTRLGVAVRAESEAVRREAEIA
                     QSLQALRDEEATRAAVLARVKVEQETFEREVERTREREAELKDRMAQLARDIEREDEH
                     IREARQILAQLDAELEADLTADQHSTQQEQAVSGELAIAQEELERVEAELQELTARAA
                     EARTKRQNLMARLAERRDEIAKLERELAGLEAQARDIAARAPEAAKLAELQELVRQLT
                     LELAELETNAVGAEERVTVAAEQAKRAREASRQAELVARQLATELATLGKLLMPAQNV
                     PYPPVVDLLEVTPGYEQALGAALGDDLEAPIAPEAAAHWRLVEAMDDEPALPEGVEPL
                     IAYVDGPPELARRLAQVGVVPRELGDKLQPALKPGQRLVSREGDLWRWDGFVAAADAP
                     TPAAPRLAERNRLVELRHREAEARELANAAAAEAIAAAERHRRAEEEERQLRQAWREA
                     QSRLAKSREQLNAIERAARETEGRLAAVAEAKERVAARLAEARERLAETEADLNGNDD
                     SENYEPLIAAAQQRARAVRETVAQLRASLAALERERKLREERAARAVSERERWLQRAA
                     SAERQIGTLKTRLDETKAELDRLAQLPPLIEEKRQALLNALSQAERERKTAADQLAAA
                     ENAQREAAQAVKAAQAAVAEEREARARVEARLEAARRRRSEEVARIRDTFNVVPEACL
                     EIAELKPGAELPKLSDVDRLLTRLRADRERLGGVNLQAEEELQSLQEQYAKLDAERVD
                     VEAAIAKLRAAIGQLNREARKRLQSAFDVVNGHFQRLFTTLFGGGEA"
     gene            complement(3169..3972)
                     /locus_tag="VFX46_02965"
     CDS             complement(3169..3972)
                     /locus_tag="VFX46_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5599575.1"
                     /translation="MRGIRLRQMITAAGLALAGVALAGCAGESSLAEALQSAGAVPVS
                     STSAGNSEFNPFAEPSGTVTPARKVIQNPTLADVLKPGPLPEMALGRADAPVTIVKYA
                     SLTCPYCRKFHLETFPQLKREYIDTGKVRFILREFPIGFQSGAATIALRCAPPERYFE
                     LYGKFLQQQAKWVSQEVRLDPIFKIASQVGMTRAQFDACYENQGMINALKEVKERGRT
                     LGVIGTPNFFINDRLVKTVIGMEEIRAMVEPLLAAHSGRLAAKDHSHSE"
     gene            complement(3981..4664)
                     /locus_tag="VFX46_02970"
     CDS             complement(3981..4664)
                     /locus_tag="VFX46_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5599576.1"
                     /translation="MSRSAIISSVKVSRRTLMVSAGLAIGALAMGSLSSELLAQRRGP
                     SEVPVEELMKPGPLPDLVLGNPDAKVTIVEYASMTCPHCASFHNNVLPELKRKYIDTG
                     QVRLIFREFPLDILAEAGSMLARCAGDKAFAMLEVLFQKQDEWVVRDDPASKLFEIAK
                     QAGFTKERFDKCLKDDELHKQVQAMRTQANQQFGVNSTPTFFINGKRLQGGGSISDFD
                     RALEPLLKS"
     gene            complement(4770..5333)
                     /locus_tag="VFX46_02975"
     CDS             complement(4770..5333)
                     /locus_tag="VFX46_02975"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEX5599577.1"
                     /translation="MRSQQTLPARAVSSYLPQLTRKAFEKYGFASTALLTDWPSIVGE
                     DVASYTAPERLKWPRGVEALDEVEPENGQRPGATLLVRVDGPRAIELQHRAQQIIERI
                     NATFGYRAVSELRFVQAPIENRGSSNRRPPPPQPSEGEAEDPNELTSISDEALRKALA
                     RLQSSMRRDIARRRRHFHARSQVLTQP"
     gene            5496..>5784
                     /locus_tag="VFX46_02980"
     CDS             5496..>5784
                     /locus_tag="VFX46_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX5599578.1"
                     /translation="MQREWDGDAAAALLDWYDAERRDLPWRAAPGTTPDPYHVWLSEI
                     MLQQTTVKAVIPYFEKFLARWPTVEALAAADLDDVLSAWAGLGYYSRARNLH"
BASE COUNT          970 a         1950 c         1730 g         1134 t
ORIGIN      
        1 gcctcaccac caccgaacaa cgtcgtgaag agccgttgga agtgaccgtt gacgacatcg
       61 aatgcagact gaagccgctt gcgggcctcc cggttgagct gaccgatggc ggcacgcagt
      121 ttcgcaattg cggcttcgac gtcgacccgc tcggcatcca gcttggcata ctgttcctgc
      181 aaggactgca gctcttcctc ggcctgcagg ttaaccccgc caaggcgctc acgatcggcc
      241 ctcaggcgtg tcagaaggcg atcgacatcg gaaagcttcg gtagctcggc gccgggcttc
      301 agttcagcga tttcgagaca ggcctcgggc acgacgttga acgtgtcccg tatgcgggca
      361 acctcttcgc tgcgccgtct ccgagccgct tccagcctgg cttcaactcg cgcacgggcc
      421 tcccgctcct cggcaacagc ggcctgagcc gctttcacag cctgggctgc ctcacgctgg
      481 gcattctctg cggcagcgag ttgatccgct gccgttttcc gctcccgttc agcctggctt
      541 agcgcgttga ggagtgcctg ccgcttttcc tcgatcaaag gcgggagctg cgcgagcctg
      601 tcgagctccg ccttggtctc gtcgagccgt gtcttcaatg tgccgatttg ccgttcggcg
      661 ctcgcagcac gctggagcca acgctctcgt tcgctcaccg ccctggcggc tcgttcctcg
      721 cgcagcttcc gctcgcgttc caaggcagca aggctagccc gcagctgcgc gaccgtctca
      781 cgcacagctc gtgcccgctg ctgtgcggcc gcgatcaagg gctcgtaatt ctcgctgtcg
      841 tcattaccgt tcaggtctgc ctcggtctcg gcgaggcgtt cccgagcctc ggctaagcgg
      901 gctgcaacgc gctccttggc ctcggcgacc gcggcgaggc ggccttcggt ttcccgagcc
      961 gcgcgctcga tagcgttgag ctgctcgcgt gacttggcga gacgcgactg ggcttcacgc
     1021 caagcctggc ggagctggcg ctcctcctcc tcagcccggc ggtggcgctc agctgccgcg
     1081 atagcctcag ctgccgccgc gttcgccagc tcgcgggcct cggcctcgcg atggcgaagc
     1141 tcgaccaggc gattacgctc tgcaaggcgc ggagcggctg gggttggagc atcagcggcg
     1201 gcaacgaaac cgtcccaacg ccacagatcg ccttcccggg agaccagcct ctggcccggc
     1261 ttgagcgccg gctggagctt gtcaccaagc tcccttggca caacgcctac ctgtgccaac
     1321 cgcctggcca gctcgggcgg accatcgacg taggcaatca ggggctccac cccctccgga
     1381 agcgccggct catcgtccat cgcttcgaca agccgccaat gggcagccgc ttccggcgcg
     1441 atgggagcct ccagatcatc gcccagcgca gcaccaaggg cctgctcgta acccggcgtg
     1501 acctccaaca gatcgaccac gggaggatag ggcacgttct gcgcaggcat aagcaacttg
     1561 ccgagggtcg ccagctccgt cgcgagctga cgagccacca gctccgcttg gcggcttgcc
     1621 tcgcgcgcgc gtttcgcctg ctcagcggct acggtaacgc gttcctccgc acccaccgcg
     1681 ttcgtctcga gctcggcaag ctcaagggtg agctgccgaa ccagctcctg aagctctgcg
     1741 agcttcgccg cctctggtgc tctcgccgcg atgtcccgcg cctgcgcttc cagcccagca
     1801 agctcgcgtt cgagcttcgc aatctcgtcc ctccgctctg ccaaccgcgc catcaagttc
     1861 tggcgcttcg tccgggcctc ggctgcacgc gcggtcaatt cctgaagctc ggcctcgact
     1921 cgctccagtt cctcttgagc aatggcgagc tccccgctaa ccgcttgctc ctgttgagtc
     1981 gagtgctggt cggcagtcag atcggcctcc agctcagcat cgagctgggc gaggatttgt
     2041 cgggcctcgc ggatgtgctc gtcctcacgc tcgatgtctc gcgcgagttg ggccatacgg
     2101 tccttgagct ccgcctcgcg ctcccgtgta cgctccacct cacgctcaaa ggtttcctgt
     2161 tcaaccttga cgcgggcgag cactgccgcg cgggttgcct cctcgtcccg caacgcctgc
     2221 agggactgcg caatctcggc ctcccggcgc acggcttcgg attcggcccg gacagcaacg
     2281 ccgagtcgcg tcgtgacctc ggttagcgcc gcctcctcag cctcgacctg ggcctgcgcc
     2341 tccttccact gcaggtagag cagcagggcc tcggcccgac ggatctcagc cgagatctcc
     2401 ttgtagcgct tcacctgccg tgcctggcgc ttcaggtttt ccagctggga gttcaactgc
     2461 cccaggatgt ccgagatacg ggccagattt gcctcggccg cgttcaggcg aagctccgcc
     2521 tcgtgtctgc ggctgtgcag gcccgcgatc ccggcagcat cctccagaat gcggcggcgc
     2581 tgctccggct tggcgttgac gatctcaccg atctgcccct ggcggaccaa ggcaggtgag
     2641 cgcgcccccg ttgccgcatc ctcgaagaga atcttcacgt cgcgcgcacg gacctcccgc
     2701 ccgttgatgc ggtacgtgga gccggcatcc cgctcgatcc ggcgtgtcac ctcgatgata
     2761 tcgctatcgt tgaattcggc aggggccttc cggtcccggt tatcgatgat gagcgtcacc
     2821 tcggccgaat tgcgcggcgg gcgcgaggtc gtgccggaga agatcacatc ctccatggcc
     2881 gaggcacgca tcgacttgta ggaggtctcg cccatgaccc agcgcagcgc ttcgagcaag
     2941 ttcgacttgc cgcagccgtt agggccgacg atccccgtaa gccccttctc gatcagaagg
     3001 tcagtgggct cgacgaatga cttgaagccg agcagccgca gccgctgaat gtgcatcctt
     3061 cgtcgtgtcc tcgcttaccg gcagcgacgc cttcgggcgg agcagcatct ccgggctgat
     3121 ctatctggga atcaggtcag gaaagtgcct gctgcgccta ttcttgcctc actcagaatg
     3181 gctgtggtcc tttgcagcga gacgaccgga atgagcagcc aatagcggct ccaccattgc
     3241 ccgaatctcc tccatcccaa tcactgtctt cacgagccga tcgttgatga agaagttcgg
     3301 cgtcccgatt acacccagcg tgcgcccccg ctccttgact tctttgagcg cattgatcat
     3361 gccctgattc tcgtagcagg cgtcaaattg cgcccgagtc atccccacct gagacgcgat
     3421 cttgaagatg ggatcaagtc gcacctcctg gctcacccac ttcgcctgct gctgcaagaa
     3481 cttgccgtag agctcgaagt agcgctccgg cggagcacag cgcaacgcga tcgtcgccgc
     3541 gcctgattgg aagcctatcg gaaactcgcg caggatgaag cgcaccttgc cggtgtcaat
     3601 atactctcgc ttgagctgcg ggaaggtctc gaggtgaaac ttacggcagt aggggcaggt
     3661 cagggatgcg tacttgacga tcgtgactgg ggcatccgcc ctccccagcg ccatctccgg
     3721 cagcggcccg ggcttcagca catcggcaag ggtcggattc tggatgacct tgcgcgcggg
     3781 cgtaacggtg ccgctcggct ctgcgaaagg attgaactct gaattacccg cgctggtaga
     3841 gcttactggc acggcgcccg cactttggag tgcctcagcc aatgagcttt cgccggcaca
     3901 ccccgccaat gcaacgccgg cgagcgcgag gcccgccgcc gttatcatct gacgcagccg
     3961 aataccgcgc acgccccgat tcagctcttc agcagcggct caagagcccg gtcgaaatcg
     4021 gagatcgagc caccgccttg aagacgcttc ccattgatga agaatgtggg cgtcgagttc
     4081 accccaaact gctggttcgc ttgggtgcgc attgcctgga cttgcttgtg cagctcatcg
     4141 tccttgaggc acttgtcgaa ccgctccttc gtgaacccag cttgcttggc gatttcgaac
     4201 agcttggagg cgggatcgtc gcgaacgacc cactcgtcct gcttctggaa caagacctcg
     4261 agcatcgcga aggctttatc gccggcgcaa cgcgccagca tggaccccgc ctcggcaaga
     4321 atgtcgagcg ggaactcgcg gaagatcaac cgcacctgcc cagtgtcgat gtacttcctc
     4381 ttcagctctg gcagcacgtt gttgtggaag ctcgcgcaat gggggcaggt catcgaggcg
     4441 tactcgacga tggtaacctt ggcgtcggga ttgccgagca caaggtccgg caatgggccc
     4501 ggcttcatca gctcctcgac gggaacttcg gatgggcccc gccgctgcgc cagcagctcg
     4561 gagctgagag aacccatggc cagagcgcca atggctagcc ccgcagaaac catcaacgta
     4621 cgacggctca ccttgacgga gctgatgatc gcggatcggg acaaggggct actccttgag
     4681 ttcataacca aacgcctaag tgccgtcaga tggcaattca taggcaattc gttaatgcaa
     4741 ttttaggctg aacgccaatt gaatggcgct cacggttgcg tcagcacctg cgatcttgcg
     4801 tgaaaatgcc tccgtctgcg cgcgatatcg cgccgcatgc tgctttggag tctagccaga
     4861 gctttgcgga gcgcttcatc gcttatgctc gtaagctcat tagggtcctc tgcctccccc
     4921 tctgatggct gcggcggtgg cggccgccga tttgacgacc ccctgttctc tatcggagcc
     4981 tggacgaatc gcaactccga caccgcccgg tagccgaagg tggcgttgat ccgttcgatg
     5041 atctgttggg cgcggtgctg cagctcgata gctcggggtc catccacccg tacgaggagg
     5101 gtcgcgcctg gccgctgccc gttctccggc tcgacttcat cgagagcctc gacaccgcgc
     5161 ggccacttca accgctccgg tgccgtgtag ctcgcgacat cctctccgac aatgctcggc
     5221 caatccgtca gtagcgcagt agaggcgaag ccatacttct cgaacgcctt acgcgtgagc
     5281 tgcggcaggt agctcgaaac tgcgcgcgcc ggcaaagtct gttgtgaacg catcgtcagg
     5341 gcctctcccg gcgaaggacg gcgattcaag ggatcgacgt tgttcacgag ttgggacctg
     5401 cgatttcttt ccctatcgct gttgcttacc atcgaccgag tgattcggga gacgcgcgga
     5461 gtgctcacgt ggcagctgtg gcgggaaggc aacgaatgca gcgcgaatgg gacggcgacg
     5521 cagctgcggc tctgctcgac tggtacgatg ccgagaggcg cgatttgcct tggcgcgcgg
     5581 ctcccggcac aacgcccgac ccctatcacg tctggctcag cgaaatcatg ctgcagcaga
     5641 cgacggtgaa ggcggttatc ccctacttcg aaaagttttt agctcgctgg ccaacagttg
     5701 aggcgctcgc agcggcagac ctcgatgacg tcctcagtgc ctgggctgga ctcggctact
     5761 acagtagagc ccgcaatctc cacc
//
LOCUS       DASUMG010000183         3638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_506223, whole genome shotgun sequence.
ACCESSION   DASUMG010000183 DASUMG010000000
VERSION     DASUMG010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506223"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..1177
                     /locus_tag="VFX46_02985"
     CDS             326..1177
                     /locus_tag="VFX46_02985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX5599579.1"
                     /translation="MRVATIALALSAVLAGTAAHASCPGKPNALGVSRTVEIDTTGGP
                     GFGFEQYRVHDFLLPKEIVLTFDDGPWPETTRAVLDALDAHCTKATFFIIGKHALWHP
                     DILKDVAARGHTVGSHTWSHANLRKMKGDKAKEEIEKGLSAVKIALDSAPAPFFRFPY
                     LQDPKEVVDYLGSRNIAIFSHDVDSFDFKFRNPESLVKSVMSKLGKKGKGIVLMHDFQ
                     KVTARAMPALLDQLAAEGYKVVHMTAKDPATTLEEFDQLAQAEVKGPVGSGRPTSSVV
                     RTISTGQ"
     gene            complement(1178..1957)
                     /locus_tag="VFX46_02990"
     CDS             complement(1178..1957)
                     /locus_tag="VFX46_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599580.1"
                     /translation="MFRRCTIPASLVLVVLVLPLPGSAPSALEFSLEIPPPVKAPSRD
                     PKKSDLSVQEFLDRLMMAESSGRLYAKNPRSTALGPFQFIRSTFLEVVRRHFPDEVAG
                     LSDEQILALRVKPDFARRAALAYTLDNASFLGDQGLDVTSVNLRLAFLVGPTGAVRVL
                     TAETDTPVSALLSADALAANPFMASMTAGDLLRKAALDLTGNDRIAGLDRPRSTIGFR
                     IPCNLGRASCRRWVALRKRKLARQQRSAAGAPSEQAAQRSQ"
     gene            complement(2147..3103)
                     /locus_tag="VFX46_02995"
     CDS             complement(2147..3103)
                     /locus_tag="VFX46_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5599581.1"
                     /translation="MFRRSLVVPFVLALLPVAAAAAWVLSPAFFDIVPASRSPEPQAD
                     VQQAALTPPELEALQPRENSDVNAPSFDVVRISPGGVSVFAGTAPPNSTVTVTADGTL
                     VGTTKADERGEWTLLSEHQFTNLDPVLGLSAAQDHQPRMAEQRGAEIKSASEKLDPPA
                     RMMRDFEDIVASARGTDQTTDHAAQRAPVPVPIQFVFRKAKFTDQGRRAVELLSEYLR
                     LKKPESIVLTGHADERGSEAFNIELSRLRLQAVADYLRQSGYEGRLVLIPKGSSEPYQ
                     GVDRSKLDRRTLWQMDRRVELNLDTDVQPHRSVQGGSSSIPR"
     gene            complement(3390..>3638)
                     /locus_tag="VFX46_03000"
     CDS             complement(3390..>3638)
                     /locus_tag="VFX46_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5599582.1"
                     /translation="TNAWNFLNKITDAAKAAGVPRYAVHIRHASPYEAIIEAANSHGC
                     DLIVMGSHGRRGIAGLLLGSETVRVLTHSKIPVLVYRE"
BASE COUNT          733 a         1029 c         1159 g          717 t
ORIGIN      
        1 tgcagggcac gtgcctcgca ccatcgatcg ttggcgagtt cgtcctgccg aactcgccat
       61 gtttgccgtt ctctacaact taagcatcct tttcgctgcc tcgtgcgaac cacgaagcag
      121 cgagacttcc gatcgctgac caattggccg cgttactcgt tacccgaaag tcaaatttaa
      181 attgatgaac ggggacagat gctccctggt aggtttttgt acatctatag gccccctcga
      241 ggctcgccag cttagcgcct ctacgtttat agtgcgaccg ggagcaggag gtaacatcta
      301 gtaagctccg ggaacggggg atgatatgcg cgttgcaaca atcgctttgg ccctctcggc
      361 cgtgctggcg gggacggctg cccatgcaag ctgtccaggg aagcccaacg ccctcggagt
      421 atcccgcacg gtggagatcg atacgaccgg tgggccaggt ttcgggttcg agcagtaccg
      481 cgtccacgat ttcctcctac ccaaagagat cgttttgact ttcgacgacg gaccgtggcc
      541 ggagacgaca cgggccgttc tggatgcgct cgacgcgcat tgcacaaagg cgaccttttt
      601 catcatcggg aagcacgcac tctggcatcc tgacatcctg aaggatgtgg cggcgcgtgg
      661 acacacggtg ggcagccata catggtccca cgccaatttg cggaagatga agggtgacaa
      721 ggctaaggaa gaaatcgaga aaggcctcag cgcagtgaag attgcgctcg atagcgctcc
      781 cgcgcctttc ttccgcttcc cgtatttgca ggatccgaaa gaggtggtcg actatctcgg
      841 ctcgcggaac attgccattt tctcgcacga cgtcgacagc ttcgacttca agttccgcaa
      901 tccggagtcg ctcgttaagt ccgtgatgag caagctcggc aagaagggca agggcatcgt
      961 gctcatgcac gacttccaga aggttacggc cagagcgatg ccggccctgc tggatcagct
     1021 tgcggcagaa ggctacaagg ttgtgcacat gaccgcaaag gacccggcca cgacgctgga
     1081 ggagttcgat cagcttgctc aggcggaggt caaggggccg gttgggtcag gtaggccgac
     1141 gtcgagcgtc gttcggacca tcagcaccgg tcagtgatca ttgagatcgc tgagctgcct
     1201 gttctgacgg agcaccggct gcggaccgct gttgtctcgc gagcttcctc ttgcgtaacg
     1261 ccacccagcg ccggcagctg gctcggccaa gattgcaggg gatccggaag ccgattgtcg
     1321 acctgggtcg gtcgaggccg gcgatccggt cgttgccggt gaggtcgagc gcagccttgc
     1381 ggaggaggtc gccggcggtc atcgaggcca tgaacggatt ggctgcgagg gcgtcggcgc
     1441 taaggagtgc cgaaactggg gtatcggtct cggctgtcag cacacggacg gcgccggttg
     1501 gtccgacgag aaaggcgagc cgtaggttta ccgatgtgac gtcgagcccc tgatcaccta
     1561 ggaatgatgc gttatccagc gtgtaagcga gcgcggcgcg gcgtgcaaaa tccggcttga
     1621 cccggagtgc gaggatctgc tcgtcgctga ggcccgcaac ctcgtcagga aagtgtcggc
     1681 ggaccacttc cagaaatgtc gacctgatga attgaaatgg tcccagcgcc gtcgaacggg
     1741 gatttttcgc gtaaagccga ccgctcgatt cggccatcat cagacgatcg aggaactcct
     1801 ggacggagag gtccgacttc ttcggatctc gtgagggggc ctttaccggc ggcggaatct
     1861 ccagactgaa ttccagggct gatggtgccg acccgggaag cggaagaaca aggacgacga
     1921 gcaccaagct ggcagggatg gtgcagcgcc tgaacatctc gcgaaggtgc ttaagaaatg
     1981 ggaaggtcct gttacggtcc atggacagac gaatgcagtc ggttcggagc ggtctggcag
     2041 gcaaacgcta gcgcctgcgt ggaagttccg acacaggacg gaaagtgggt gattgaaccc
     2101 acgtcagacg gaggagccta ctgattgcag tggggcttgc cgcgggtcag cgcggaatgc
     2161 tggagctgcc accctgcacg gagcggtggg gctggacgtc cgtatccaga ttaagctcca
     2221 ctctacggtc catttgccaa agtgttcgcc ggtcgagttt tgatcgatcg acaccctgat
     2281 agggctcgga gctgcccttg ggaatgagca cgagtctgcc ctcgtaccca ctctgccgca
     2341 ggtagtccgc aactgcctgg agccttagac gggacagctc gatgttgaag gcctcgctgc
     2401 cgcgctcatc ggcgtgcccg gtcaggacga tgctctcggg cttcttcagt ctcagatatt
     2461 cgctgagcaa ttcgaccgcc cggcggccct ggtccgtgaa ctttgctttc cggaacacga
     2521 actggatcgg taccggcacg ggggctcgtt gagcagcgtg atcggtggtc tgatccgtcc
     2581 ctcgggccga cgcaacgata tcctcgaaat cgcgcatcat acgcgcaggt ggatcgagct
     2641 tctcagatgc ggatttgatc tcagcacccc tttgctccgc cattcgcggc tggtggtcct
     2701 gagctgctga caatccaagg accgggtcga ggttcgtgaa ctggtgctca gacagcagtg
     2761 tccactcgcc acgctcgtca gctttggtcg taccaacgag tgtgccatcg gccgtgaccg
     2821 tcaccgtcga gttgggcggt gcggtgccgg cgaacaccga tacgccccct gggctaatgc
     2881 gcacgacgtc gaaggacgga gcgttcacgt cgctgttttc gcgcggctga agcgcttcga
     2941 gctcgggtgg cgtcaaggcc gcctgctgaa catccgcctg cggctccggc gaccgagatg
     3001 ctgggacaat gtcgaaaaat gcgggcgaaa gaacccatgc ggctgctgct gcaacgggca
     3061 gcagcgcgag cacaaaggga acgacgaggc tgcgacggaa catcgaagta cgcctcaaca
     3121 taagactttg tatcacctcg agcccagtgc ttacaaggtc tcgcaatgta tttgtggaga
     3181 ggcggctgtc aaactagggg gtgccatccg aactggagag aattgcgggg cgcactattg
     3241 gatcgatact cccgcgaaag ctcgcaggaa acttgggttc agctataggt ggagctgcaa
     3301 ctcgttaata aaagttaacg gagccatacc caccatgggc acggctccgc acccgctgcc
     3361 gaaaaggaca atccgtgaga gtgccccgtc tactcgcgat acacgagcac cgggatcttg
     3421 ctgtgggtta ggacgcgcac ggtttcgctg ccgagcagga gtccggcaat tccccgacgc
     3481 ccatgagagc ccatgacgat gagatcgcag ccgtgcgaat tggcggcttc gatgatggcc
     3541 tcgtaggggc ttgcgtgtcg tatgtggacc gcataacgtg gaacccccgc cgcttttgcg
     3601 gcatcggtga tcttgttgag gaagttccag gcgttggt
//
LOCUS       DASUMG010000184         6203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1148446, whole genome shotgun sequence.
ACCESSION   DASUMG010000184 DASUMG010000000
VERSION     DASUMG010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148446"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFX46_03005"
     CDS             complement(<1..129)
                     /locus_tag="VFX46_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HEX5599583.1"
                     /translation="MAETPEEFDYVIVGAGSAGCILANRLSADPSVRVLLLEAGGKD"
     gene            complement(170..793)
                     /locus_tag="VFX46_03010"
     CDS             complement(170..793)
                     /locus_tag="VFX46_03010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5599584.1"
                     /translation="MSSAPGLSGSPNAVTIARLREAPDSGSASEAIEAIFFESSGRRF
                     DNEASRAEFLERWLGRYLTHYPEYVFVALAPGGEFVGYLVGCLDDPASNPLFSDISYF
                     AEFAHLTRKYPAHLHINLTARWRGQGIGKRLVATFAECAAKVGVPGLHVITAEGHRNN
                     RFYVACGFVQLGSAVWNGNRIAFFGRALVRSDDCSRDPDLPRCDPLG"
     gene            917..1141
                     /locus_tag="VFX46_03015"
     CDS             917..1141
                     /locus_tag="VFX46_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599585.1"
                     /translation="MSTPNTTIDSALDFVSSGTRTGRPRGVVQAIRDFFASLRTGIEA
                     AHHYERLKAQGVPPQEAVEIVYRDHFAKKN"
     gene            complement(1371..2357)
                     /gene="pta"
                     /locus_tag="VFX46_03020"
     CDS             complement(1371..2357)
                     /gene="pta"
                     /locus_tag="VFX46_03020"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX5599586.1"
                     /translation="MDIIAECKASIRGRGLRVILPESDDDRVLRAARRLLDEDLARPV
                     LLGVPESIEGKADELELSLDGVEIRNPDGDPDLEGLAAKIATGRERMTTGMAMRLLRR
                     PLYYGGAMVASGQAAAMVAGAANPTRRVIEAGLMTVGLAEGVATPSSFFLMLLPATHD
                     GAPRAYVFADCAVNVDPDASTLADIAIASAASAEALLHQEARVAMLSFSTHGSASHPI
                     VEKVRAAVGIVRERKPDLKIDGELQADAALNGTVAAKKIKGESAVAGRANVLVFPNLD
                     AGNIAYKLVQYLANAQAIGPFLQGFAKPIADLSRGASVDDIVATAAVTLARA"
     gene            2447..3592
                     /locus_tag="VFX46_03025"
     CDS             2447..3592
                     /locus_tag="VFX46_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5599587.1"
                     /translation="MSAADNEPKIYRGLKDVYFERSPTTFIDGKAGELRYRGYSIHDL
                     AEHSTFEETAYLLLYGELPTRSQLVKFEEDLKAARQLPPAVLDIISVVKHAHPMDVLR
                     TAVSALAAFDPETADNSREATLRKGIRLTSQVPMIVAAHEQIRKGRTPIAPDPQLSHA
                     ANFLWMLKGEKPSQDAARLLDTDFVLHAEHGSNASSFTARVVISTEANIHAAITAAIA
                     ALSGPAHGGAAEDVMKMAQEIGDPASVPAYVKAKRAAKEPITGFGHRVYRAEDPRARH
                     MREGVKRLSKEMGEPRWYEILEALVKEMEPYARHGVNVNVDFYSGVIYHLHGIPTDLF
                     VPIFAIGRVPGWTVQVLEQQSNNILIRPLTLYNGPGPRDYVPIDQRG"
     gene            complement(3646..3993)
                     /locus_tag="VFX46_03030"
     CDS             complement(3646..3993)
                     /locus_tag="VFX46_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599588.1"
                     /translation="MTKRLLSLAAAAALLIVVTPGPEVRAAAAKVPMLKTEASDIVEV
                     RHRKWRGYSYKRRHFRRHHHWRHRRHWHRHWHGPRFAIYIGGPRCGWLRRRAILTGSR
                     YWWRRYYRCRYWW"
     gene            complement(4153..4473)
                     /locus_tag="VFX46_03035"
     CDS             complement(4153..4473)
                     /locus_tag="VFX46_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599589.1"
                     /translation="MRLWSVIAQRKLLRRARMPIGILLAVFLSGCATSSEYGEYWTGS
                     ERVAMEDDGLPAQLPPPLHRKSEVDDPREPFSPHYGPPPPPSLPADLPPAFRRKLINA
                     VSVS"
     gene            complement(4633..>6203)
                     /locus_tag="VFX46_03040"
     CDS             complement(4633..>6203)
                     /locus_tag="VFX46_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX5599590.1"
                     /translation="SLLNEATEGLKRGDVLPGEVAFKLYDTYGFPLDLTQDALKPRGI
                     AVDIDGFNEALERQKEEARKAWKGSGEAATEAVWFELREEVGATEFLGYETESAEGVI
                     RGIVKDGSRVDRLKAGEEAAIVLNQTPFYGESGGQVGDTGEIRGAKGAIFRVTDTQKK
                     LGDLFVHYGVVEKGQFAVGDAVELEVDHARRSAIRANHSATHLIHEALRQVLGSHVAQ
                     KGSLVAPDRLRFDFSHPKPMTAEELKKVEDLANSIVAQNVPVETHLMSLEEALETGAM
                     ALFGEKYGDEVRVVSMGLAPPGSNRPWSMELCGGTHVKRTGDIRIIRIISEGASAAGV
                     RRIEALTGDGARAYLNQQDDRVREAANVLRVRPDDLVDRVKALMEERRTLERQLADAK
                     RQLALGAGGNGVAAADGIRTIGNIKLLARKVEGLKPKDLRGLVDDGKKKVGSGIVAIV
                     GVTEDGKAGIAVGVTDDLTNTYNAVDLVRAGAEVLGGKGGGGRPDMAQAGGPDGSKAH
                     LALEAIERRLSGAA"
BASE COUNT         1231 a         2027 c         1825 g         1120 t
ORIGIN      
        1 atccttgcca ccggcctcga gaagcagaac gcggacactc gggtcggctg aaagtctgtt
       61 ggccaatatg cagcccgccg agccggctcc cacgatcacg taatcgaact cttccggggt
      121 ttcggccatc gcgtttcgct cctccctggc atctcatgtt gaaacgtcat taccccagcg
      181 ggtcgcatcg aggaagatca ggatcgcggg aacaatcgtc actccgtacg agcgcccggc
      241 cgaagaacgc gattcggttg ccgttccaaa cggcggagcc gagctggaca aatccgcacg
      301 ccacatagaa ccggttattg cggtgccctt ccgccgttat cacatgcagg ccaggaacgc
      361 cgaccttcgc tgcacactct gcaaaagtcg cgacaagccg cttgccgatc ccctgaccgc
      421 gccagcgggc cgtcagattg atgtgcaggt gtgctgggta cttccgcgtc agatgcgcga
      481 attcggcgaa ataggagatg tccgaaaaaa gaggattgct ggcgggatca tcgaggcaac
      541 cgacgagata gccgacaaac tcacctccgg gagcgagcgc tacgaaaaca tattccgggt
      601 agtgcgtaag gtagcggccc agccaccgct caagaaactc agcccgagag gcctcgttgt
      661 cgaagcgccg gccactggac tcgaagaaga tcgcctcaat tgcctcgcta gcgctccctg
      721 aatctggtgc ttccctcagc cgtgcgatgg tcacggcatt tggcgagcca gataggccgg
      781 gtgcagaact catctatgca gaccacgaat agcaggtaga cagaatgcca cattgtgcaa
      841 cgcaatgtgg tatgccatat accagttcga tgagccgaga gccggaacag gcccggagag
      901 cgaacgaggt ttgatcatga gcaccccaaa tacgacgatc gactctgcct tggactttgt
      961 gtcctccggc acgagaactg gccgcccgcg cggcgtggta caggcgatcc gcgacttttt
     1021 cgcgtcgctt cgcaccggca ttgaggcggc tcaccactac gagcggctca aggcgcaagg
     1081 ggtacctccg caggaggcgg tcgagatcgt ctaccgcgat catttcgcga aaaagaactg
     1141 atcgcgaaca ttccccagac taagatccca aactcctcgc cgtgcagcct gcacggcttt
     1201 ttttatttgg gcccgagctc agcggcattg acaggggcgc ccggactcct tctagatgca
     1261 atctgatatt cgagacaact tgtcccggat tttagactat gggcgccatc ccgggaattc
     1321 agagcgcggt tgccagcaga ccagcgaagc ccatcctgcg gcgccagcgc tcaggccctt
     1381 gcaagggtga ccgcggctgt ggcgacaatg tcgtcgacgc tcgcgccgcg cgagagatcc
     1441 gcgataggct tggcgaaacc ctgcagaaaa ggtccgatcg cctgggcgtt ggccagatac
     1501 tgaaccaact tgtaggcgat gttccccgcg tccaggttcg ggaacacgag tacgttggcg
     1561 cggccagcca cggcgctctc ccctttgatc ttcttggcag cgacggtgcc attcagcgcc
     1621 gcatcggcct gcagctcccc gtcgattttc aggtcgggct tccgctcgcg cacaatgccc
     1681 accgccgcgc gaaccttctc gacaatcgga tggctggcgc taccgtgggt ggagaaggac
     1741 agcatcgcca ctctcgcctc ttgatgcagc agcgcctcgg cactggcggc ggaagcgatc
     1801 gcgatgtcag ccagcgtgct ggcatcgggg tccacgttca cggcgcaatc ggcgaagaca
     1861 tacgcccgcg gagcgccgtc gtgagttgcg ggcagcagca tgaggaagaa gctcgacggc
     1921 gtggcgaccc cctctgccag gccaacggtc ataagccctg cttcgatgac acgccgcgtt
     1981 ggattagcgg cgccggccac catggcagct gcttggccgg aggcaaccat agcgccgccg
     2041 tagtagagcg gccggcggag caatcgcatg gccatgcctg tcgtcatgcg ctcccggccg
     2101 gtggcaatct tggcagcgag gccttcgaga tccgggtccc catcgggatt acggatttca
     2161 acgccgtcca gggacagctc gagctcgtcc gccttgcctt caatcgactc cggcacgccc
     2221 agcagcacag gccgcgccaa gtcctcatcg aggagacggc gcgcggcacg caacacacgg
     2281 tcatcgtcgc tttcaggaag aatcaccctc agcccgcgcc ccctaatcga ggccttgcac
     2341 tcggcaatga tgtccatgtc ggccctctcg aaagctcctt ggcaggcagt tggcagccgc
     2401 ccgcgtcctc actggcatcg caattccgga agggaggttc aagcttatga gcgccgcaga
     2461 caacgagccc aagatctacc gtggtttgaa ggacgtctac ttcgagcgat cccccacgac
     2521 atttatcgac ggaaaggcgg gtgaattacg ctaccgcggc tattctattc atgacctcgc
     2581 cgagcattcg accttcgagg aaacagcgta cttgctgctc tatggtgagc tgccgacccg
     2641 cagtcagctc gtcaagtttg aggaggacct caaggcggcg cgtcaactcc caccggccgt
     2701 attggacatc atctccgtcg tgaagcacgc ccatcccatg gatgttctgc gtacggctgt
     2761 ttcggcgctc gcagcattcg atccggagac ggcggacaac agccgcgagg cgacgttgcg
     2821 caaaggcata cggctgacct cccaagtgcc gatgatcgtt gccgcgcatg agcagatccg
     2881 aaaaggacgc acgccgattg cgcccgaccc gcagctctcg catgccgcca atttcctctg
     2941 gatgctgaag ggcgagaagc cttcgcaaga tgcggcgcgg ttgctcgaca cagattttgt
     3001 gctgcatgcc gagcacggct cgaatgcctc ctctttcaca gcccgtgtcg tgatcagcac
     3061 cgaggccaac atccatgccg ccatcacagc ggccatcgct gcactttccg gcccggctca
     3121 cggcggcgct gcggaggacg tgatgaagat ggcgcaggaa atcggcgatc ccgccagcgt
     3181 gccggcctat gtgaaggcaa agcgcgcagc aaaggagccg atcaccggct tcggccatcg
     3241 cgtttaccgc gccgaggacc cgagggcgcg tcacatgcgc gaaggtgtca agcggctgtc
     3301 gaaggaaatg ggcgagccgc ggtggtacga aatcctagag gcgctcgtga aggaaatgga
     3361 gccctacgcc cgccacggcg tcaacgtcaa cgtcgacttt tactcaggcg tgatctatca
     3421 cctgcacggc attccgaccg atctgttcgt cccgattttc gccatcggcc gtgtgccggg
     3481 gtggaccgtg caggtcttgg agcagcagtc caacaacatc ctcatccggc cactgacgct
     3541 ctacaatggc cctgggccgc gggactatgt ccctattgat cagcgcggat gaaatctgcc
     3601 caaaagaaaa ccgggccgaa aattcggccc ggcttggatt cctgcttacc accaatagcg
     3661 gcagcgataa tagcgacgcc accaatagcg gctgccggtc agtattgccc gacgacgcag
     3721 ccacccgcac ctcggcccgc cgatataaat cgcaaaccga ggcccatgcc agtggcgatg
     3781 ccaatggcgg cggtggcgcc aatggtgatg gcggcggaaa tggcgcctct tgtaagaata
     3841 tccgcgccat ttgcgatgtc gaacctcgac gatatccgac gcctcggtct tcagcatcgg
     3901 aactttagca gccgcagcgc ggacctccgg ccctggtgta acgacgatga ggagggcagc
     3961 agcagccgct agggaaagca gacgcttcgt catttccaga ctcctgaaca gttgtgcgcc
     4021 ggctcagggt tcggccgacg acgaaccgct gtctctttgc tgagcttccc cacgtgatcg
     4081 cagtacccta atacggataa cctcgagatc caacccgggt ttcagttgca tccgcattga
     4141 cgccgatcaa aatcacgaga cacttaccgc attgataagc ttacggcgga acgccggtgg
     4201 caaatcagcg ggcaacgacg gcggaggcgg tggcccgtag tgcgggctga acggctcacg
     4261 ggggtcgtct acttccgatt tccgatgaag gggtggcgga agttgggcag gaagtccgtc
     4321 atcctccatg gcgacgcgct cactgccagt ccaatattcg ccgtactcgg atgaggttgc
     4381 acagccggaa agaaagaccg cgaggagaat gccaatgggc attctggcgc ggcgcagcag
     4441 ttttctctgc gcgataaccg accacaaacg cataacccga actccactga cacgacactg
     4501 tcgatcatca gagctcaggc agggttaaga ccgggttagc cctcgtccca attcaataca
     4561 ggttcgaatt ttccaccccc gcgcggccga gaaatgagca gcttgagcaa agctggtgag
     4621 caagggccat ggttacgcgg ccccgctcag ccgccgctcg atagcctcta gtgcgagatg
     4681 ggccttgctg ccgtccggcc cgccagcttg agccatatca gggcgaccgc caccaccttt
     4741 gccgcccagc acctcagcac cggcccggac gagatcgacg gcgttgtacg tgttcgtcag
     4801 atcgtccgtc acgccgacgg caatgccggc tttgccatcc tctgtcacgc ccactatggc
     4861 gacgatgccg gagccgactt tcttcttccc gtcgtcaaca agcccgcgga gatccttcgg
     4921 cttgagccct tcgactttcc gcgcgagcag cttgatgttg ccgatggtgc ggatgccgtc
     4981 cgcagcagcc acaccattgc cgcccgcgcc cagcgcgagc tggcgtttgg catccgccag
     5041 ctgtcgctcc aacgtccgcc gctcctccat cagcgccttg acgcgatcga ccaagtcatc
     5101 aggccgtacc cgaagcacat ttgcagcttc gcgcacgcga tcatcctgct gattgaggta
     5161 agcgcgcgcg ccatcgccag tcagcgcctc gatgcggcgc acaccggcag cggacgcgcc
     5221 ttcggaaata atccggataa tgcggatgtc gccggtgcgc ttcacgtgcg tgccgccgca
     5281 cagctccatg gaccacggcc ggttggagcc cggaggcgcc aggcccatgg acacgacgcg
     5341 cacctcatcc ccgtacttct cgccgaagag cgccatggca cccgtttcca acgcctcctc
     5401 gagcgacatc aggtgcgtct cgaccggcac gttctgcgcg acgatggagt tcgccagatc
     5461 ctcgaccttc ttcaactcct ccgccgtcat gggcttcgga tgcgagaagt cgaagcgcag
     5521 gcgatcgggc gcgacaagcg agcctttctg cgcgacgtgc gatccgagca cctgccgcaa
     5581 cgcttcgtgg atgaggtgag tcgccgaatg gttggcgcgg atggctgaac ggcgcgcgtg
     5641 atcgacctcg agctcgaccg catcgccaac cgcgaactgc cccttctcga cgacgccgta
     5701 gtggacgaag aggtcgccga gcttcttctg cgtgtcggtg acgcggaaga tcgccccctt
     5761 ggcgccacgg atctcgcccg tatcgccgac ctgtccgccc gactcgccat agaacggcgt
     5821 ctgattgaga acgatcgcag cctcctcgcc agctttcagg cgatcgacac ggctgccgtc
     5881 cttcacgatg ccacgaatga cgccttccgc cgattccgtt tcataaccaa ggaattcggt
     5941 ggcgccgacc tcctcgcgca gctcgaacca cacagcctcg gtcgcggcct cgccggagcc
     6001 cttccaggct ttccgcgcct cctccttctg ccgctccaga gcctcgttga agccatcgat
     6061 gtcgacggca atcccgcgcg gcttgagcgc gtcctgcgtg aggtcgaggg ggaagccata
     6121 cgtgtcatag agcttgaagg ccacctcgcc cggcagcacg tcgccccgct tcaggccttc
     6181 ggtcgcctca ttgagcaggc tta
//
LOCUS       DASUMG010000185         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_24414, whole genome shotgun sequence.
ACCESSION   DASUMG010000185 DASUMG010000000
VERSION     DASUMG010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24414"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFX46_03045"
     CDS             <1..502
                     /locus_tag="VFX46_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX5599591.1"
                     /translation="PHSPWAALDAFQLGEPRRHSLDVVVEGQKTSVEVLWNGGSPSVA
                     LPGEPGPIQCDEINAKAIDDGQRVFVLHNLRQTEVAWPVYDVSAADESDGTGSVRAPI
                     NGRVVKVFVEEGTLLDKGQRIAVIEAMKMEHVLVAPIRGRIAKLTASEGQQVTEGTVL
                     ATLVEE"
     gene            511..1515
                     /locus_tag="VFX46_03050"
     CDS             511..1515
                     /locus_tag="VFX46_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX5599592.1"
                     /translation="MVPGPRNLITDIPGLRVGNAHDERVLSGVTAVIFDKPNVASGVT
                     LGGAPGSRDTTLLEPEMTIQSLDAVVLSGGSLFGLDAAGGVVSFLRQEGRGVRIGTAN
                     VPIAAQAIIFDLLNGGDKNWGRMPPYWELGWRAAEKASGGDFPLGTVGGGYGASTVNF
                     KGGLGSASAVTPSGFIVGALAIVNAVGSVTIGDGPHFWAGAYEIGREFGGHGLPSRVP
                     AEAHKLRIKGTAPPSTTIALVATNARLTKAEAKRLSIMANDGLARAIRPVHAPMDGDT
                     VIAVSMQDAPLKNRTTDLTEIGQAAADCLARAVARGVYEAAVPSDAWAGPPAYRDLYP
                     "
     gene            1512..1703
                     /locus_tag="VFX46_03055"
     CDS             1512..1703
                     /locus_tag="VFX46_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599593.1"
                     /translation="MTRARWAIVIGVTALLVALLVWQQERQREVQRCIDSGGIWDGPT
                     SRCVPDPARPILQRDLQRT"
     gene            complement(1709..>2518)
                     /locus_tag="VFX46_03060"
     CDS             complement(1709..>2518)
                     /locus_tag="VFX46_03060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5599594.1"
                     /translation="ERYDQYLREQEEVRREFGVNFEQLDPAALRKREPYLTGNFAGGL
                     LMLDPVSVADPSAVGKAYADLFVRRGGKFITADARTLESTIDGWQVHTVDGPVKAREA
                     VIALGPWSDDVLRPLGVRVPLWVKRGYHIHFRPRGDAVLNGPLFDIESGFVLAPMTHG
                     IRLTTGAEFAHRDAPKTPVQLEKLEPIARAMFPLADRVENKPWMGSRPCLPDMLPMIG
                     PIPGHRGLWADFGHHHLGFTLGPATGRLLAEMLTGEPTFTDPWPYRVDRFR"
BASE COUNT          470 a          747 c          854 g          447 t
ORIGIN      
        1 accgcattcg ccttgggcgg ctctcgatgc attccagctg ggcgagccgc gtcgccattc
       61 gctcgacgtc gtggtggaag gccaaaaaac ctccgtcgag gtcctgtgga acggaggcag
      121 tccaagcgtc gcgctgccgg gcgagcctgg tccaatccag tgcgatgaga tcaacgctaa
      181 ggccatcgac gatggacagc gcgtgttcgt actccataac ttgcggcaaa cagaggtcgc
      241 ctggccggtt tatgacgtca gtgccgcgga cgaaagcgac ggcactggga gcgttcgtgc
      301 gccaattaac ggccgtgtgg tgaaagtctt cgtggaagaa ggcacgcttc tcgacaaagg
      361 gcagcggatt gcggttatcg aggcgatgaa gatggagcac gttctcgtcg cgcccattcg
      421 tggaaggatc gccaagctga ccgcgagcga agggcagcag gtgaccgagg gaaccgtgct
      481 ggcgacattg gtcgaggagt aggcaaagta gtggtgcccg gcccccgcaa tttgattacg
      541 gacattcctg gtttgagagt cgggaacgcg cacgacgagc gcgtgctgag tggcgttaca
      601 gctgtcatat tcgataagcc gaacgtggct tctggcgtaa cgctcggggg ggctccgggc
      661 agccgcgata cgacgctgct cgagcctgaa atgacgatcc agagcctcga tgcggtcgtg
      721 ctctcgggcg gctcgctttt cgggctggat gccgcgggtg gcgtcgtctc gttcttgcgc
      781 caagaggggc gcggcgtgcg tattggcacg gcgaacgtgc ccatcgccgc acaggcgatc
      841 atattcgacc ttctaaacgg gggtgacaag aactgggggc gtatgccccc gtactgggag
      901 ctcggctggc gggcggcgga aaaggctagt ggcggcgatt tcccgctggg caccgtcggt
      961 ggcggctatg gggctagcac tgtcaacttc aaaggcggcc tcggctctgc gagcgcggtg
     1021 acgccatccg ggttcatcgt cggcgcgctc gccattgtga atgccgttgg atcagtgacg
     1081 attggcgacg gaccgcactt ctgggccggt gcttacgaga tcggccgcga atttggggga
     1141 cacggcttgc caagtcgcgt accggcggaa gcccataaac tcaggatcaa aggcacggca
     1201 cccccctcga cgacgatcgc tctcgttgcg acaaatgccc ggctgaccaa ggcagaggcg
     1261 aagcggcttt caatcatggc caatgatggg ctggcccgtg cgatacgacc ggtccatgcg
     1321 ccaatggacg gcgatacggt aatcgcagtc tcgatgcagg atgcgccgct caagaatcgg
     1381 acgaccgatc tcaccgaaat cggccaagcc gcggccgact gcttggcccg tgctgtcgcc
     1441 cgtggcgtgt acgaagccgc cgtgccaagt gacgcgtggg ccggcccccc ggcctaccgg
     1501 gacctctatc cgtgacacgt gcccgctggg cgattgttat cggcgtaacg gcgctgctgg
     1561 tcgcgttgct ggtctggcag caggagcggc agcgcgaggt ccagcgctgc attgattccg
     1621 gcggaatctg ggatggcccg acttcccgct gcgtccccga tccggcgcgt ccgattttgc
     1681 agagagacct ccagcgcacc tagcctcttc accggaagcg atccacccga taaggccacg
     1741 gatcggtaaa cgtgggttcg cctgtcagca tttcggcaag caggcgcccg gttgcagggc
     1801 cgagcgtaaa gccgagatgg tggtggccga agtcggccca aagaccccga tggcccggga
     1861 taggaccgat cataggcagc atgtcgggca ggcagggccg ggagcccatc cacggcttgt
     1921 tttcgacccg atctgcaagc gggaacatgg cgcgggctat cggctcgagc ttctcgagct
     1981 gcacgggcgt tttcggcgcg tcgcggtggg cgaactcagc acccgtggtc aggcggatgc
     2041 cgtgggtcat cggcgccaac acgaaaccgc tttcgatgtc gaagagaggt ccgttgagca
     2101 cggcatctcc acgcgggcgg aagtggatat ggtaaccgcg cttcacccag agcggaacac
     2161 ggacgccaag cggcctcaaa acgtcatccg accaagggcc gagtgcaata acggcttcgc
     2221 gcgctttgac cggaccgtcg accgtgtgaa cttgccagcc gtcaatagtg gactcgagcg
     2281 tgcgcgcatc ggccgtgatg aacttgccgc cgcgtcgcac gaagaggtcg gcatacgcct
     2341 tgccgacagc gctgggatcg gccacgctga ccgggtccag catcaggagg ccgcccgcga
     2401 agttgccggt gaggtagggt tcgcgcttac gcagagcggc aggatcgagt tgttcgaagt
     2461 taacgccaaa ttcgcgccgc acctcctcct gttcacgcaa atattggtcg tagcgttc
//
LOCUS       DASUMG010000186         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_345926, whole genome shotgun sequence.
ACCESSION   DASUMG010000186 DASUMG010000000
VERSION     DASUMG010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345926"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VFX46_03065"
     CDS             complement(<1..791)
                     /locus_tag="VFX46_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX5599595.1"
                     /translation="MIQFAIIGIGAGAAAALLFASVTSGSLLSVPLYCLAPLPIMIAG
                     LGWSHWAAMTAALVGALGLGAVFGTVFLLAFSAGVGFPAWWLGYLAMLARPVAASNGS
                     SQGAVSFEWYPPGRLVVWAAVLGVLVVIVAIPNFGTDADSFRAGLRDALNAIMRVNNG
                     TLARAGSNTERLLEILVNAIPPAAAVLATVTNLLNLWLAARVVKFSGRLARPWPQLSS
                     MTFPPLMTAALGVAVVLSFLDGMLGIVGGIVAASLLLVYGVLGFAV"
     gene            complement(885..1133)
                     /gene="rpsR"
                     /locus_tag="VFX46_03070"
     CDS             complement(885..1133)
                     /gene="rpsR"
                     /locus_tag="VFX46_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5599596.1"
                     /translation="MAFAPQGGARRPFFRRRKTCPFSGANAPKIDYKDVKLLQRYVSE
                     RGKIVPSRITAVSAKKQRELAQAIKRARFLGLLPYVIQ"
     gene            complement(1137..1574)
                     /gene="rpsF"
                     /locus_tag="VFX46_03075"
     CDS             complement(1137..1574)
                     /gene="rpsF"
                     /locus_tag="VFX46_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5599597.1"
                     /translation="MPLYEHVYLARQDLSAQQVEELTAQLSGVIGQMGGKVTKNEYWG
                     LKSLNYRIRKNRKAHMTLLNVDAPPEALNEIERQERLHEDVLRYLTIRVDALEDGPSA
                     MMRKSDRDRDDRGGRDDRGGRGERRRRDEGDDIDSPDTIGGEE"
     gene            1881..>2665
                     /gene="fabD"
                     /locus_tag="VFX46_03080"
     CDS             1881..>2665
                     /gene="fabD"
                     /locus_tag="VFX46_03080"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5599598.1"
                     /translation="MAIAFVFPGQGSQAVGMGKALASNFTAAQRIFEEVDEALGSKLS
                     TIIFEGPAETLTLTENAQPALMAVSLAVQRVLETEAGVDLARDVQFVAGHSLGEYSAL
                     AAGGAFTVADAARLLRTRGQAMQKAVPVGVGAMAALIGLEFDAAAAVAAEAAQGEICQ
                     AANDNGAGQVVVSGNKAAVERAVEIAKTKGAKRAMLLPVSAPFHCALMQPAADVMAEA
                     LAKTTVKPPTVPLIANVLAKPIRAPDEIVRALVAQVTGTVRWR"
BASE COUNT          542 a          820 c          828 g          475 t
ORIGIN      
        1 actgcaaatc cgagcacgcc atagaccaag agcaaacttg ccgcgacaat gcctccgaca
       61 atgccaagca tgccgtccag aaagctcaaa acgacggcaa cgccgagtgc tgcggtcatc
      121 aacggtggaa aggtcattga cgaaagctgc ggccagggac gagccaaacg gccggagaat
      181 ttcacgacgc gtgccgcaag ccagaggttg agcaaattgg tgactgtcgc gagcacagca
      241 gctgctggcg ggatcgcatt gaccaggatt tctaatagtc gctcggtgtt gctcccggcc
      301 ctcgccagcg tgccgttgtt gacacgcata atggcgttga gcgcatcacg caggccggca
      361 cggaagctgt ctgcatcggt cccgaaattg ggaatggcga caataacgac cagaacgccc
      421 aacacggccg cccatacgac cagcctgccg ggcggatacc actcaaacga cacagcaccc
      481 tggctgctgc cgttgctggc ggcaactggg cgggcaagca ttgcgagata accgagccac
      541 caagccggga aaccgactcc ggcagaaaac gcgagcagaa agacggtgcc aaaaaccgcg
      601 ccaagcccaa gcgcgcccac aagtgccgcg gtcatggcgg cccaatggct ccagccgagg
      661 cccgcgatca tgatgggcag cggtgcaaga caatataacg ggaccgacag caacgagccg
      721 gaagtcaccg aggcaaacag caaagccgcc gcggcgcccg cgccaatccc gataattgcg
      781 aactgtatca tcacgagctg tcccgctcct cactgagcgg ttagagacgg agttgctccg
      841 ccccaaccat cggcgcccgg gtgtccccgg acgccttgtt gcgcctactg tatcacatac
      901 ggcagcaggc cgagaaagcg cgcacgtttg atcgcctgcg ccagctcgcg ctgtttctta
      961 gctgaaaccg ctgtgatgcg actcggaacg atcttgccgc gttccgaaac ataccgttgc
     1021 agcagcttca catccttgta gtcgatcttg ggtgcattgg cgcccgagaa tggacaagtc
     1081 ttacggcggc ggaagaaggg gcggcgtgca ccgccctgtg gcgcgaatgc catggtctat
     1141 tcctcccctc caatggtgtc aggactgtcg atgtcgtcgc cttcgtcacg ccgacggcgt
     1201 tcgccgcgtc caccacgatc gtcgcgaccg ccgcgatcgt cgcggtcgcg gtccgatttg
     1261 cgcatcatcg cggagggacc atcctcgaga gcatcgacgc ggatcgtgag gtagcggagc
     1321 acatcttcat ggagacgttc ctggcgctcg atttcgttga gcgcttccgg cggcgcatcg
     1381 acattgagca gcgtcatgtg cgccttgcgg ttcttgcgga tgcggtagtt gagggacttc
     1441 aggccccaat attcgttttt ggtgacctta ccgcccattt gcccgatgac gccggacagc
     1501 tgcgcggtca attcctcgac ctgctgcgcg ctcaggtcct ggcgcgcaag gtagacgtgt
     1561 tcgtacaacg gcatgaatgc ctttcccttg tttcaagtcc tcggtcctcg atcccggcgc
     1621 caagcccctc gagcccttcg gggaaaggct cgaatgctct gaaggcggaa gcacgggacg
     1681 ccgggccaag tggccctgcc gggtgacaat cgtgcccaac cggccgtccg ttcagctccc
     1741 aaccaggatc gatggagcgc gcggtttata ggccgttttt gcaaaagtgc aagataaaac
     1801 aaagggaccc gcattggctt gacatgtcgg ctatcgcggt gtctttcggc gcgatttccg
     1861 cacagattga cggatagggc atggcgatcg ctttcgtatt tccggggcaa ggcagccagg
     1921 ccgtagggat gggcaaggcc ctcgcatcaa atttcaccgc cgcgcagcgg atattcgaag
     1981 aggttgacga agcgcttggc agcaagctca gcaccatcat tttcgaagga ccagctgaga
     2041 cgctgacgct tacggaaaat gcccagccag ctcttatggc tgtctcgctt gccgtccagc
     2101 gtgtccttga gaccgaagcg ggagtggacc tcgcgcgcga cgtacagttt gtcgccggtc
     2161 attcgctcgg tgaatattcc gcgttggccg cgggcggcgc gttcactgtg gcggatgccg
     2221 cgcgtctcct tcggacgcgg ggccaggcca tgcagaaggc agtcccggtc ggtgtcggcg
     2281 cgatggcggc gcttatcggg ctcgagttcg acgcggccgc tgccgttgcg gctgaagccg
     2341 cgcagggcga aatctgccag gcggcgaatg acaacggcgc cggccaagtg gttgtttcgg
     2401 gaaacaaggc agccgtggaa cgcgcggtag aaatcgcaaa aacaaaaggc gcaaagcgtg
     2461 cgatgctgtt gcccgtgtcg gcacccttcc attgcgcctt gatgcaaccg gccgccgatg
     2521 tgatggctga agcgctcgcg aagacgacgg tcaagccgcc cacggtcccc ttgatcgcca
     2581 atgtgctggc gaagcccata cgcgcgcccg acgaaatcgt gcgcgctctc gtggcgcagg
     2641 tgaccggaac ggttcgctgg cgtga
//
LOCUS       DASUMG010000187         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1148630, whole genome shotgun sequence.
ACCESSION   DASUMG010000187 DASUMG010000000
VERSION     DASUMG010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148630"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFX46_03085"
     CDS             <1..333
                     /locus_tag="VFX46_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX5599599.1"
                     /translation="DWGQTDLYRGVRAPFLGLVAKLPAFQDQKLIEEGITDWTDQMQR
                     LDAHLSEHGPYLMGDSFTLADVPVGLIVNRWFGVPFDKPELKAVSAYYDRLAERPAYR
                     LHGRNGTP"
     gene            433..933
                     /locus_tag="VFX46_03090"
     CDS             433..933
                     /locus_tag="VFX46_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599600.1"
                     /translation="MKRQALSVLLLTPALIAPATAGEVCVTCSGPPAIYRCTVDDASR
                     LEGYRHSKRILQIACITELARTGGHQQCRVQRTGSESCFGFRRTISLADTLESLAARA
                     ENEPVEESAVEQQPAVSQQKDSGPPRTVEELARRTADSSKEQLKKTGKAVKQGWSCLT
                     SLFQDC"
     gene            1146..1391
                     /locus_tag="VFX46_03095"
     CDS             1146..1391
                     /locus_tag="VFX46_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599601.1"
                     /translation="MVWRCAALAAAVALLAAGCAQDNGTAVFGYQAALPPQAADITPD
                     MEAQLRKKTLASKVLAAIALERVTGRKPDPSRLTELN"
     gene            1631..>2340
                     /locus_tag="VFX46_03100"
     CDS             1631..>2340
                     /locus_tag="VFX46_03100"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX5599602.1"
                     /translation="MRLATFNVESLDLPPRAKLPIEVRAEALRPVLARLDADILCLQE
                     VNGQHVPDRPERVLLALDLVLKGTPYASYERISTSGPDGLGVADVHNLVTLSRFPIRS
                     RREVFHDLVPQPLYRMVTADPRPSEPEPVTFDRPLLAADIELPSGQLLTVINVHLRAP
                     LAAAISGQKLSAFVWRTVGGWAEGYFLASMRRAGQALELRLLLEELFAADPHRLIAVA
                     GDFNAEDYDTALRIAIGA"
BASE COUNT          430 a          736 c          738 g          436 t
ORIGIN      
        1 gactggggtc aaactgacct ttaccggggc gtccgcgccc cattcctcgg tctggtcgcg
       61 aagttgcccg ccttccagga ccagaagctc atcgaagaag gcatcaccga ttggaccgac
      121 cagatgcaaa ggctcgatgc ccatctgtcg gagcacggcc cctatctcat gggtgacagc
      181 tttacgctgg cagacgtccc ggtcggtctc atcgtcaacc gctggttcgg cgtcccattc
      241 gacaagccgg agctgaaggc ggtgagcgcc tattacgatc ggctggccga gcgtcccgcc
      301 tatcgcctgc acggacggaa cggaacaccc taatcggact tgacggggtg ccttcgggag
      361 gcgccccact ctcgccctgt ttttcttctt ggaatctggg ccttactggt cgtcagggag
      421 gcttgcgcga tcgtgaaacg gcaggcgctt tctgtccttc tgctaacacc ggcgctgatt
      481 gcacccgcta cagccggaga ggtctgcgtg acgtgttccg gtccaccggc gatctaccgg
      541 tgcacagtcg acgatgcgag caggcttgag ggctatcggc acagcaagcg catccttcag
      601 attgcgtgca ttaccgagct tgcaagaaca ggcggccatc aacaatgccg cgtgcaacgg
      661 accgggtccg agtcgtgctt cggatttcgg cgcacgatat ccctggcaga tactctcgag
      721 tcgctggcgg cccgcgcaga aaacgagccg gtcgaggagt cggcggtcga gcagcaacca
      781 gcggtctcac agcaaaagga ttccggcccg ccacggaccg tggaggagct tgcacgccgc
      841 accgcagact cctccaagga gcagctcaaa aagacgggca aggctgtcaa gcaaggttgg
      901 agctgcctca cctcgctgtt ccaggactgt tgagcggcgg aaattgatgc gcccaattcg
      961 cagcgacatc gcggccgcgg gacgttgcgt agaaccttca gtatattcag caccggttca
     1021 cgcgaagtct gcctatgctt gcgtgatggc ttcgatccgc tagagtgtcg gccgcaaacg
     1081 gcggcgcctc tgtcgggtgc ccatagccca tccgatttgt tggggttgat ttggaggggg
     1141 gggtcatggt ctggaggtgt gctgcgcttg ctgctgctgt tgcgctgctc gcggctggct
     1201 gcgcgcagga caacgggaca gcggttttcg gctaccaggc cgcgctgcca ccccaggcgg
     1261 cagacatcac ccccgacatg gaagcgcagc tccgcaagaa gacactggca agtaaagtgc
     1321 ttgctgctat cgcactcgag cgggtcacgg gccgcaagcc tgacccatca cgcctcaccg
     1381 agctcaactg acgcgtgccc cgagcacatc agggcgaccg tgaagcctgc tccagccttt
     1441 ccggaggcta accccttaca cctggagatg gcagaaagcc ctctgcggca gctggatttg
     1501 cccttcatca tggccgattg cgttgaaggg agacaggcag gtcgccagcc ccccaagacc
     1561 gaccggtgga ataattcacg gggtggtgtg gtacgggtca ggatgaggac cggccagtgt
     1621 ggagcacggc atgcgactgg cgacgttcaa tgtggagagc ctcgatcttc cgccgagggc
     1681 aaagctaccg atcgaagttc gcgcagaagc tttgcgcccc gtcctcgcca ggctcgatgc
     1741 cgacattctc tgcctgcagg aggtgaacgg gcagcatgtg ccggatcggc cggaacgagt
     1801 gctcctggcc ttggacctgg tgctgaaggg cacgccatac gccagttacg aacgcatctc
     1861 gacaagcggg ccggacggct tgggcgtcgc ggatgtgcat aacctcgtga cgttgtcccg
     1921 ctttccaatc cggtcgcggc gcgaggtgtt tcacgatctg gtgcctcagc cgctctacag
     1981 gatggtaacc gccgacccgc gcccatccga gccggagccg gtgacctttg atcgccccct
     2041 gctcgctgcc gacatcgagc tcccgagcgg ccagctgctg accgtgatca acgtccatct
     2101 gagggcgcca ctcgccgcgg ccatttctgg acaaaaactg agcgcctttg tgtggcggac
     2161 cgttgggggc tgggcggaag ggtactttct cgcctccatg cggcgtgccg gacaggccct
     2221 cgagttgcgt ctactcctcg aagagctgtt tgcagccgat ccgcaccgcc taatcgcggt
     2281 cgccggcgac ttcaacgcgg aggactacga tacagccctg cgcatcgcca tcggagcgga
//
LOCUS       DASUMG010000188         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_506712, whole genome shotgun sequence.
ACCESSION   DASUMG010000188 DASUMG010000000
VERSION     DASUMG010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506712"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VFX46_03105"
     CDS             <1..201
                     /locus_tag="VFX46_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX5599603.1"
                     /translation="KRAKAISANPLDEIPGIGPARKRALLQHFGSAKAVSRAGVADLK
                     EVPGISEQMAQTIYDFFHERRH"
     gene            complement(251..1210)
                     /locus_tag="VFX46_03110"
     CDS             complement(251..1210)
                     /locus_tag="VFX46_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5599604.1"
                     /translation="MGAVQSAIETGAVDDSGLPIIDAHHHYWDLGLNRHPWLAPDVLI
                     PFRYGDYSAIKTNFMPADYRAVSARHRIVASVTMEGEWDENDPVAETRWITEVAASHG
                     TPAAHVARAFLHRADVEEVLAGHAAYPIVRGIRHKPTAAAAPGLIEAGAPGSMGDPAW
                     RRGYAMLQEHGFHFELQAPWWHVDELLDLIAAYPETPVVINHTFLPADRSPEGLAGWR
                     AALKRAASAPQVTIKISGIGLKGRPWSIDDNRPIIRETIEIFGPDRCMFASNFPVDGL
                     CGSFDTIYSGYKQAVADLPTPDKLKLFHDNAIRIYRLAIPKLT"
     gene            complement(1220..2203)
                     /locus_tag="VFX46_03115"
     CDS             complement(1220..2203)
                     /locus_tag="VFX46_03115"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5599605.1"
                     /translation="MIGRRTFLSGIGGLALSLAAAWSALAQSYPNRPVQMIVPWAAGG
                     GTDTVARIFAAGLEKELGQPVNVVNRTGGNGMTGHNAIASATPDGYTVGIATSEIASY
                     KTLGLGDITPDSFDLVSRITTIPAGITVKADGPYKTAQDVIDALKASKKDELSASGTG
                     VGGAWHLAVAGLNKSIGLPADHVKYVPSQGGAPALNELAAGGLSFLTVSPIEALALAQ
                     AGRVKVLAVMDTKRLSSYPDVPTLKEATGLDWTFVNWFAFVLPKGIPDDVRTKLIEAA
                     ARAHARPEVQEALKARGITPVWETPEEFRAFATKFANTTAELLTDLGLAKN"
     gene            complement(2245..3279)
                     /locus_tag="VFX46_03120"
     CDS             complement(2245..3279)
                     /locus_tag="VFX46_03120"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5599606.1"
                     /translation="MGDPAGISPELTAKLLADADIRAAANLIAFGDRRVLAEGERIAG
                     VNADIPVWTPGMAIPDRAGSALFADLGHLDPAAITLGEATATGGRFALTNFRAALKLA
                     AAGGAGAVVYTPFNKYAMRLALPSYEDEIAVITETIGAEAHGREFNVLGNLWNARVTS
                     HVPLRRVADLITKEAIVNALVLTDKCMRSAGFERPRIAVAALNPHAGDGGNFGHEEID
                     VIRPAIEEAKARGVACEGPFPSDTVFLRARRGDFDAVQTMFHDQGQIAMKLMGFEEGV
                     TLIGGYPFPICTPAHGTAYDIAGKGIAKLGATRQAILMAARMANTPSAGPGRIRELLE
                     DICRAVALTV"
BASE COUNT          621 a         1130 c         1089 g          643 t
ORIGIN      
        1 aagcgggcga aagccatttc ggccaacccg ctcgacgaga tccccggcat tggaccggcg
       61 cgcaaacgcg cactgctgca gcacttcggt tcggccaagg cggtgtcacg ggccggcgtc
      121 gccgacctca aggaagtgcc tggcatctca gagcagatgg cgcagaccat ctatgatttc
      181 ttccacgaac gcaggcattg accgcctgtg acttcatttg gcgcgcctat cccactttgc
      241 acgtgcccta tcaggtgagc ttcggaatgg cgagccggta aatgcggatg gcgttgtcgt
      301 ggaagagttt cagtttgtcg ggagtcggaa gatcggcgac ggcctgcttg tagcctgaat
      361 agatcgtgtc gaagcttccg cacaaaccat cgacggggaa gttcgacgcg aacatgcagc
      421 gatcggggcc gaaaatctcg atcgtctcgc ggatgatggg gcggttgtcg tcgatgctcc
      481 acggcctgcc cttaaggccg atacccgaaa tcttgatcgt cacctgcgga gccgaagccg
      541 cacgcttgag cgccgcccgc cacccggcta gaccttccgg actacggtct gctggcagga
      601 acgtgtggtt gatgacgacc ggcgtctcgg gataggcggc gatcaaatcg agaagctcat
      661 ccacgtgcca ccagggcgcc tgcagctcga aatgaaagcc gtgctcctga agcatggcgt
      721 accctcgccg ccacgccgga tcacccatgg agcccggcgc cccggcttct atcaggcccg
      781 gtgcagcagc agccgtcggc ttatggcgaa tgccccgcac gatcgggtac gccgcatggc
      841 cggcgagcac ttcctcgaca tcggccctgt gcaggaatgc ccgcgcgacg tgggctgcgg
      901 gcgtgccgtg tgatgcggct acttctgtga tccagcgggt ttccgccact gggtcgttct
      961 catcccactc gccttccatc gtgacgctcg ccacgatgcg atgccgcgcc gacacagcgc
     1021 ggtagtcggc cggcatgaag ttggtcttga tcgccgagta gtcgccgtac cggaagggga
     1081 tcaggacgtc gggtgccaac cacggatgcc ggttgagccc gaggtcccag tagtgatggt
     1141 gggcgtcgat gatcggcaac ccgctgtcgt ccacggctcc cgtctcgatc gcggattgca
     1201 ctgctcccat cggaggcgct cagttcttcg ccaatcccag atcggtcagc agctcggcgg
     1261 tcgtgtttgc gaacttcgtc gcgaaggctc tgaactcctc cggtgtctcc cataccggcg
     1321 tgatgccgcg ggccttgagc gcttcttgaa cctcaggcct agcatgtgcc ctggcggctg
     1381 cctcaatcaa cttcgtgcgc acgtcgtcgg gaatgccctt cggcagcacg aatgcgaacc
     1441 agttgacgaa ggtccaatcg agacctgtgg cctccttgag cgtgggcaca tccgggtagc
     1501 ttgaaagccg cttggtgtcc atgacggcga gcaccttcac ccggcccgcc tgggccagcg
     1561 ccagcgcctc gatcggcgac actgtgagga atgagaggcc ccctgcggca agctcgttga
     1621 gggctggcgc gccgccctgg ctcggtacgt atttcacatg gtcggcgggc agcccaatcg
     1681 acttgttgag acctgcgacg gcgagatgcc acgcaccacc aacgcctgtg ccagaagcgg
     1741 acagctcatc cttcttggac gccttgagcg cgtcgatgac atcctgagct gtcttgtacg
     1801 gaccgtcagc ctttaccgtg atcccggccg gaatggtggt gatgcgcgag accagatcaa
     1861 acgagtccgg agtgatgtct ccgaggccga gcgtcttata ggaagcgatc tccgacgttg
     1921 cgatgccgac cgtgtagccg tccggcgtgg cggacgcgat ggcgttatgg ccggtcatgc
     1981 cattgccacc ggtgcgattg accacgttga cgggctgacc gagctccttc tccagcccag
     2041 ctgcgaagat ccgggccacc gtatcggtcc cgccgcctgc agcccacggc acgatcatct
     2101 gcaccggccg gttcgggtag gattgcgcta gcgccgacca tgcagccgcg agggacaatg
     2161 cgagtccccc gatgccagac aaaaatgtcc gtcttccaat cattgtttcc tcccatgttg
     2221 ttattctcgt ctgcaaggca gggatcagac ggtaagcgcg actgcccggc agatatcttc
     2281 cagcaattcg cgaatgcggc cggggccggc tgacggtgtg ttcgccatac gtgccgccat
     2341 cagaatagcc tgccgcgtag cacctagctt ggcgatgccc tttccagcga tgtcgtaggc
     2401 ggtcccatga gccggcgtgc agatcggaaa gggatagcca ccgatcaggg tgaccccctc
     2461 ctcgaagccc atcagcttca tcgcgatctg gccctggtcg tggaacatcg tctgcactgc
     2521 atcgaagtca ccccgccggg cgcggagaaa gacggtgtcg gaaggaaacg ggccttcaca
     2581 ggcaacgcct cgggccttcg cctcttcgat tgccggccgg atcacatcga tctcctcgtg
     2641 gccgaaattg ccgccgtcgc cggcatgagg gttaagcgca gcgaccgcga tgcggggccg
     2701 ctcgaatccg gcgctgcgca tgcatttgtc cgtgagcacg agcgcgttga cgatggcctc
     2761 cttggtgatc agatcggcaa cgcgccggag cggcacgtgc gaggtgacgc gcgcattcca
     2821 caggttgccc agcacgttga actcgcgccc gtgcgcctcg gcaccgatcg tctcggtgat
     2881 gacggcgatc tcgtcctcat agctcggcag cgccagacgc atcgcgtact tgttaaacgg
     2941 cgtgtacacc acagcgcctg ccccgccggc agctgccaat ttcagggcgg cgcggaagtt
     3001 cgtgagcgca aagcgcccgc cggtagcggt cgcctcgccc aacgtgatgg cggctggatc
     3061 gagatgtccg agatcggcga acagggctga gcctgcccga tcgggaatgg ccattcccgg
     3121 cgtccagacc gggatatcgg cgttgacgcc cgcgatcctt tcgccctccg ccaacacccg
     3181 ccgatcgccg aaagcgatga gattcgcagc agcacgaatg tccgcgtccg caaggagctt
     3241 cgcggtcagc tcagggctga tccccgcggg gtcacccatc gcaagcgcga tcacagggcg
     3301 ttcggaggtt tccgtcacgc gatcctctcg ggctcgatac tgacactatg cttagcacgg
     3361 ggccctggtc cgcaagcaat aggcccgcac gcttgaggct tggccgaaac gccatttccg
     3421 cgacgagcaa ggctcccggt ccgtaggggg caacggcgat ccacctcacc caccgctatt
     3481 cga
//
LOCUS       DASUMG010000189         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_827499, whole genome shotgun sequence.
ACCESSION   DASUMG010000189 DASUMG010000000
VERSION     DASUMG010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827499"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1359)
                     /locus_tag="VFX46_03125"
     CDS             complement(<1..1359)
                     /locus_tag="VFX46_03125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX5599607.1"
                     /translation="MISNSLRVRLLGAAVAIIVLAVQFAGLVVLILFERNVISLVHNE
                     LDAELDQLAASLSLNGDGKPSLTRELPDVRFRQLFAGRYWQLSVGDRPVLRSASLGDS
                     SLHVEVAANADAWRQPLAGPDKQKLYASIRRIVPLPPRHEKGGAPYLMIVAMDVAEIS
                     TLDTLNSQLRSNIFIVLGLLALILIAAAWAQVSIGLHPLETLRTGVENIRVGNARRLS
                     DDVPIEVQPLVTESNRLLEAQEKAIETARARAGDLAHGLKTALTVLATVAERMRERRE
                     HDLAEEMQQQLIVLNAHVERELTRTRIAANSTVMQRTLLKPLAERLLRTMERLPRGDR
                     LNWRLDCSPDFAAPVEEVDLAEILGNLLDNARKWARSEVTVAAWAAAGDAQLAIDDDG
                     PGVSASDRDNVMQRGVRLDESVPGSGLGLSITRAAVEAYGGKMALEASSTGGLRVLLR
                     FPD"
     gene            complement(1356..2018)
                     /locus_tag="VFX46_03130"
     CDS             complement(1356..2018)
                     /locus_tag="VFX46_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599608.1"
                     /translation="MRVLVVEDEAALAASIKGSLERSGFRVDLADNGEDAWVLGDTED
                     FTAVILDLGLPRMDGLSVLKRWRTTGRRMPVVILTARGTWMERVEGIDAGADDYLSKP
                     FQMEELLARLHAVLRRSAGQAMNTVTIGSLVIDQRRKSVTADGVPVELTPLEFSLLCC
                     FVLRPGEIISASELLDQVYGHGSEKDANVLEALLGRLRRKIGSDVILTRRGQGYFLRV
                     DG"
BASE COUNT          437 a          721 c          682 g          439 t
ORIGIN      
        1 gtcgggaaag cgcaagagca cccggaggcc gccggtcgac gatgcttcga gagccatctt
       61 gccgccgtat gcttcaacag ctgcgcgtgt tatgcttagc cccaatcccg agccgggaac
      121 gctctcatca agtctcaccc ctctttgcat cacgttgtca cgatcagacg cggacacacc
      181 gggtccgtca tcgtcgatgg cgagttgcgc gtcgccagct gccgcccaag cggcaacggt
      241 cacttccgat cgcgcccatt tgcgtgcgtt gtccagcaga ttgccaagaa tttccgccaa
      301 gtcgacttcc tcgaccggcg ctgcaaaatc ggggctgcaa tcgagcctcc aattgagccg
      361 gtcaccgcgc ggaagtcgtt ccatcgtccg cagcagccgc tcagcgagcg gctttaggag
      421 cgttctttgc atcaccgtcg aattggccgc aatgcgcgtt cgcgtcagct ctctttcgac
      481 atgggcgttg agcactatca gctgctgctg catctcctct gcaaggtcat gctcacggcg
      541 ctcacgcatt ctctctgcaa ccgtcgcgag caccgtcaga gccgtcttga ggccgtgggc
      601 cagatcgccg gctcgcgcac gagcggtctc gatcgccttc tcctgtgcct caagtaaccg
      661 gttcgactcc gtcaccagcg gctgcacctc gataggaaca tcgtcggata gccttcgtgc
      721 attgccgacg cgaatgttct ccacccccgt tcggagggtc tcgagcgggt gcagccctat
      781 agacacctgc gcccaggcgg ccgcgatgag gatcaaggcc agcagtccga gtacgatgaa
      841 gatgttgctg cgaagctgtg aattgagggt atcgagcgtg ctgatctcgg ccacgtccat
      901 agcgacaatc atcaagtagg gcgcccctcc cttttcgtgc cggggcggga gtggaacgat
      961 gcgccggatc gatgcataga gcttctgttt gtcgggcccg gcgagaggtt gtcgccaggc
     1021 gtcagcattt gcggcaacct cgacgtgaag cgagctatcg cccagcgaag cggaacgcaa
     1081 caccggtcga tcgccgacgg acaactgcca atatcggccg gcgaagagtt gacggaaacg
     1141 tacgtccggt agctcgcggg ttagcgatgg ttttccgtct ccgttaagag acaaggacgc
     1201 ggcgagctga tcaagctccg catcgagctc gttgtgcacg agcgatatga cattcctctc
     1261 aaagaggata aggaccacca gccctgcgaa ctggacggcg aggacgatga tggcgactgc
     1321 ggcgccgagc aaccggacgc gtaatgaatt cgagatcaac cgtctaccct gagaaaatat
     1381 ccttggccgc ggcgcgttaa gatgacgtcg gagccgatct tgcggcgcag ccgcccgagc
     1441 aaggcctcaa gaacattggc gtccttttcg ctgccgtgac cgtaaacctg gtcgagaagc
     1501 tcggatgcgc taatgatctc gccgggtcgc agcacaaagc agcaaaggag actgaactcg
     1561 agcggtgtta gctcgaccgg tacaccgtcc gccgtgaccg acttgcgtct ctggtcgatg
     1621 accaggctgc caatcgttac tgtgttcatc gcctgtcctg cggatcgccg aaggacggca
     1681 tgcaggcgcg ccagcaattc ttccatctgg aacggctttg acagatagtc atcggcgccc
     1741 gcatcgatgc cttcgacacg ctccatccag gttccgcggg ccgtcaggat gaccacgggc
     1801 atacgccgcc cggttgtccg ccagcgcttg aggacgctca gcccgtccat gcggggaagg
     1861 ccgagatcga gaatcacagc agtgaagtcc tcggtatccc cgagtaccca agcatcctcg
     1921 ccgttgtcag cgagatccac cctgaagccg ctgcgttcga gcgagccttt gatgctagcg
     1981 gcaagcgcgg cctcgtcctc gacgactaga accctcatcg ccacctcagc acaaagtgcc
     2041 ccaatctggg gatgttgggc gcgttggtgc aagcactggt atagtagcat tgccgggaac
     2101 gaaatccaca gcttccgtcc gtgcttgcgg gcgccgacgg gcgccgcagc ggtctggtaa
     2161 agtccgcagc cggtggtcca ccgcggcttc ccgaggagtc tgtgcggtca gcacgccgac
     2221 ctgacagggc cgggtcggca tccactcgcc gtcttcctgt gcagcgcttt gcgtttgct
//
LOCUS       DASUMG010000190         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_747657, whole genome shotgun sequence.
ACCESSION   DASUMG010000190 DASUMG010000000
VERSION     DASUMG010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747657"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFX46_03135"
     CDS             complement(<1..296)
                     /locus_tag="VFX46_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5599609.1"
                     /translation="MAKLNVNGKQYDVDVDPETPLLWVLREQIGLTGTKYGCGIAVCG
                     ACTVHINGEAQRSCSIPVSALTETDQIVTIEGLSEDSSHPVQRAWLELEVPQCG"
     gene            complement(669..>3012)
                     /gene="pepN"
                     /locus_tag="VFX46_03140"
     CDS             complement(669..>3012)
                     /gene="pepN"
                     /locus_tag="VFX46_03140"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEX5599610.1"
                     /translation="EANKALQGLYRSRGIYCTQCEPEGFRRITYFVDRPDVLAVYTTR
                     IEADAEEAPVLLSNGNPVERGTIDGGKRHYAVWKDPFPKPSYLFALVGGNLSAVTSTF
                     RTKSGRKVDLAIYVEPGKENRCGWAMDSLKRAMRWDEERFGLEYDLDVFNIVAVSDFN
                     MGAMENKGLNIFNDRLILVSPETATDAIYEAVESVVAHEYFHNWTGNRITCRDWFQLC
                     LKEGLTVYRDQEFSADERSRTVQRISDVRQLKAHQFPEDAGPLAHPVRPESYIEINNF
                     YTATVYEKGAEVIRMIEQIVGRDGFRAGMDLYIKRHDGQAATVEQFVKCFEDANGVDL
                     SQFMLWYKQAGTPELICSLDYNEARKEAKLTVEQVLKPTPGQPNKKPMHIPLRLGLLG
                     GNGEDIDLNLADSGPVPDGVIHVKKRKQTFRFTHVPSKPVPSLLRGFSAPVKLNIDLS
                     DRDLAFLMANDSDLFNRWQAANTYAMRMLIQLVKARTEERRPAQAVAYAQALGASLRN
                     EDLEPAYRAELLKLPSENDIAREIAKNVDPDAIHRARKRLLRIIGETLGAELEDIYKS
                     FDDGGKFSPDAASAGRRALRNAALTLLSARNTAADKVRLADHYWKASNMTEAAHALYL
                     LAARGGTDAKKALDDFYQRWKGDHLVIDTWFAAQAISPLAGTLARVKALTRHPLFSLT
                     TPNKVRALIGNFAAQNLVQFNRPDGAGYEFVVEQVLAIDKFNPQIAARMLSGFRSWRE
                     LEPGRRKLARKALRQVAKAKGLSRDVTEIVTKMLEAPPAD"
BASE COUNT          564 a          902 c          898 g          648 t
ORIGIN      
        1 tacccgcact gaggcacttc gagctccagc cacgcccgct ggaccggatg tgagctgtcc
       61 tcggacaagc cttcgatcgt gacgatctgg tccgtttccg tcagagccga aactgggatc
      121 gagcacgagc gctgcgcttc cccgttgatg tgaactgtgc aggcaccgca cacggcgatg
      181 ccgcaaccgt atttcgtgcc tgttaatcca atctgttcac gaagcaccca gagcagcggc
      241 gtctctgggt cgacatcgac atcgtactgc tttccattga cgttgagttt cgccatgggt
      301 cagcgtcctc ccttgggggg tgcatgcttt aggtgtgcta ggccgcgtcg gcgctcaccg
      361 cgtgcgccgg aactcatcag actatccagt ctattggccg aatatggctt ggcaaccgcg
      421 tttggcggga agcggttgat ggagaacttg cttctatcgc cccgagagat tgaaaaagct
      481 gattttgtcg aggtttctga cgagacttga catttttcaa atcggcgtga gcaccgctcg
      541 ggcccattcc gcaggcactc caccctcgtc atcccggcgc ttgtcgccgg gatccaaata
      601 tccacttgca catgagctcg gggtcagtgg attccggggt gcaaggctcg ggatgaccac
      661 gagagtgttc aatctgccgg aggagcctca agcattttgg tgacgatttc ggtcacgtcg
      721 cgggacagcc ccttcgcttt cgccacctgc cgcaaagcct ttctcgcgag cttgcggcgg
      781 ccaggctcca gctcacgcca actccggaaa cccgagagca ttcgggcggc aatctgcgga
      841 ttgaacttgt cgatggcgag cacctgttcg acgacgaatt catagccggc gccgtccggc
      901 ctgttgaact ggaccagatt ttgggcggcg aagttgccga tcagcgcgcg gaccttgttc
      961 ggcgtggtta gcgagaacag cggatgccgc gtgagcgcct tcacccgcgc cagcgtaccg
     1021 gcaagcggtg atatcgcctg ggctgcgaac caagtgtcga tgacgagatg gtcacccttc
     1081 cagcgctggt agaagtcatc aagcgccttc ttggcatccg taccgccccg cgcagccagc
     1141 agatacagcg cgtgcgcggc ttctgtcatg ttggaggcct tccagtagtg gtccgccaat
     1201 cgcaccttgt ctgccgcggt attgcgggca ctgagcagcg tgagcgccgc gttgcgcaat
     1261 gcgcggcggc cagcgcttgc ggcatcgggc gagaatttcc cgccgtcatc gaatgatttg
     1321 tagatgtcct cgagttcggc gcccaacgtt tcgccgatga tgcgcaaaag ccgcttccgg
     1381 gctcgatgaa tggcgtccgg atcgacattt ttcgcaattt cacgggcgat gtcgttttcg
     1441 gacggcagct tcagcagctc tgcgcgataa gcaggctcga gatcctcatt gcgaaggctc
     1501 gccccgaggg cctgggcata agcgacagcc tgcgccgggc gccgctcctc cgtgcgcgcc
     1561 ttgacgagtt gtatcaacat gcgcatcgca taagtgttcg cagcctgcca gcgattaaag
     1621 aggtcgctgt cattggccat caagaatgcg aggtcccggt cgctgagatc gatgttgagc
     1681 ttcaccggag ccgagaaacc gcgcagcagc gacggcacgg gcttcgaggg tacgtgcgtg
     1741 aagcggaacg tctgcttgcg cttcttcacg tgaatgacgc cgtccggcac tggtccggaa
     1801 tcggcaaggt tgagatcgat gtcctcacca ttgccgccga gaagccccag ccgcagcgga
     1861 atatgcatcg gctttttgtt gggctgccct ggcgtcggtt tgagcacttg ttcgacggtg
     1921 agcttcgctt ccttgcgcgc ctcgttgtaa tcgagcgagc agatgagctc cggcgtgccg
     1981 gcctgtttgt accagagcat gaattggctg aggtcgaccc cgttggcgtc ctcgaagcat
     2041 ttcacgaatt gctcgacagt cgcggcctgc ccgtcgtggc gcttgatgta gaggtccatg
     2101 ccggcgcgga agccgtcccg gccgacgatc tgctcgatca ttcgaatgac ctcggcgccc
     2161 ttctcgtaga ccgtcgccgt atagaaattg ttgatctcga tatagctctc cggccgcact
     2221 gggtgggcca acgggcctgc atcctccggg aactgatgcg ccttcaattg tcgaacatct
     2281 gaaatgcgct gcaccgtgcg cgaacgctca tcagctgaga attcctgatc gcggtagacc
     2341 gtcaatcctt ccttcaagca gagctgaaac cagtcgcggc aggtaattcg gttacccgtc
     2401 cagttatgga agtactcgtg cgcgacaacg gattcgacgg cttcgtaaat cgcatccgtc
     2461 gccgtttccg gcgaaacgag aatgaggcgg tcgttgaaaa tattgagccc cttgttctcc
     2521 atcgccccca tgttgaagtc ggacacggcg acgatgttga acacgtcgag atcatactcg
     2581 agcccgaacc gctcctcgtc ccagcgcatc gcgcgcttga gcgaatccat cgcccagccg
     2641 cagcggtttt cttttccggg ctcgacgtaa atggcgagat cgactttgcg gcccgatttc
     2701 gtccgaaatg tcgatgtgac ggcggataag tttccgccga cgagtgcaaa caaataagag
     2761 ggtttcggga agggatcctt ccacaccgcg taatgccgct tgccgccatc gatggtcccg
     2821 cgctccacgg gattaccgtt cgacagcaga acaggcgcct cttcggcatc cgcttcgatg
     2881 cgtgtcgtat agacggcgag cacatcgggg cgatccacga aatacgtgat gcggcgaaac
     2941 ccttccggct cgcactgagt gcagtagatg ccgcgggagc gataaagacc ttgcagcgcc
     3001 ttattggctt cc
//
LOCUS       DASUMG010000191         3247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1069059, whole genome shotgun sequence.
ACCESSION   DASUMG010000191 DASUMG010000000
VERSION     DASUMG010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069059"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1516
                     /gene="secY"
                     /locus_tag="VFX46_03145"
     CDS             164..1516
                     /gene="secY"
                     /locus_tag="VFX46_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX5599611.1"
                     /translation="MASAAEQLAANLNFGAFAKAEELKKRIWFTLGALVVYRLGTFIP
                     IPGVDPVAFAQAFQSSQSGILGMFNMFAGGAVERMAIFALNIMPYISASIIMQLMSSI
                     NPTLEQLKKEGEQGRKQINQYTRYLTVVLAAFQAYGIAIGLEGTRAASGAVVIEAGWF
                     FRLTTVVTLVGGTMFLMWLGEQITARGVGNGISLIIFAGIVATLPSAIAALFELARTG
                     SLSTIMLLLLLALMLLVVVVIVFMERAQRRIPIQYPKRQIGNRIFQGDSSHLPLKLNS
                     SGVIPPIFASSLLLLPITAAQFMSGQGPEWLNTIVAALGRGQPLHLIIYVALIVFFTF
                     FYTAVVFNPQETAENLRKYGGFILGYRPGQKTAEYIDYVLTRITVVGAIYLSAVCVLP
                     EFLISYAGVPFYFGGTSLLIVVSVTMDTVAQVQSHLLAHQYEGLIKKAKLRGATRGRR
                     "
     gene            1513..2172
                     /locus_tag="VFX46_03150"
     CDS             1513..2172
                     /locus_tag="VFX46_03150"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX5599612.1"
                     /translation="MNLILLGPPGAGKGTQAATIAASRGLIQLSTGDMLRAAVQAGTE
                     LGKKVKPIMESGQLVPDELVIGIIDERIAQPDCENGFILDGFPRTLPQAAALDELLEK
                     RNKQLDLVIELKVDDAVLIERISGRFACAKCGAGYHDKFKQPKTPGVCDACGSTEFVR
                     RADDNAETVRTRLMAYYKETAPLIGYYFAKGKLKSINGMAPIEEVTRDIERLLDEVVA
                     A"
     gene            2378..2749
                     /gene="rpsM"
                     /locus_tag="VFX46_03155"
     CDS             2378..2749
                     /gene="rpsM"
                     /locus_tag="VFX46_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX5599613.1"
                     /translation="MARIAGVNIPTQKRVVIALQYIHGIGPKFAKEICEKVGIPPERR
                     VHELTDAEVMQIRETIDRDYVVEGDLRREVAMNIKRLMDLGCYRGLRHRRGLPVRGQR
                     THTNARTRKGPAKPIAGKKKA"
     gene            2821..3219
                     /gene="rpsK"
                     /locus_tag="VFX46_03160"
     CDS             2821..3219
                     /gene="rpsK"
                     /locus_tag="VFX46_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX5599614.1"
                     /translation="MAREQQARTKVRRKERKNISSGVAHVNATFNNTMITITDAQGNT
                     IAWSSAGAMGFKGSRKSTPYAAQVAAEDAGRKAIEHGMKILEVEVSGPGAGRESALRA
                     LQAVGFQLTAIRDVTPIPHNGCRPRKRRRV"
BASE COUNT          630 a          970 c         1043 g          604 t
ORIGIN      
        1 agctcagcac ctttcgggcg aaggtcgtcc cgcgttatct tgctgccagc gccggccgtt
       61 gcataagagg gcgacaggca ggcggcgcca gggacgcggc tcgtaaggct tgcatactga
      121 ctgctgccgg gccgcgtcgg aaatttcggg agattcgtag aaaatggcat ccgccgccga
      181 acaactggca gcaaatctga atttcggcgc ctttgccaag gccgaggagc tgaagaagcg
      241 catctggttc acgctgggtg cgctcgtcgt ctatcggctt ggcacattca tcccgattcc
      301 gggcgtcgat ccggtggcct tcgcgcaggc cttccagtcg agccagagcg gcatcctcgg
      361 catgttcaac atgttcgccg gtggtgccgt cgagcgcatg gcgatctttg cgctcaacat
      421 catgccctac atctcggcct cgatcatcat gcagctcatg tcgtcgatca atccgacgct
      481 tgagcagctg aagaaggagg gcgagcaggg ccgtaagcag atcaaccagt acacccgcta
      541 cctgacggtc gtattggcgg cgttccaggc ctacggcatt gcgataggtc tcgagggaac
      601 acgagcggcc agcggcgctg ttgtcatcga ggcaggttgg ttcttcaggc tgacgaccgt
      661 cgttacgctg gtcggcggca ccatgttcct gatgtggctc ggcgagcaga taaccgcgcg
      721 cggggtcggg aacggcatct cgctgatcat tttcgcgggc atcgtggcga ccttgccgag
      781 tgccattgcc gcactcttcg agctggcacg gacgggctcg ctgtcgacga tcatgctgct
      841 tcttctgctg gcgctgatgc tgctggtcgt cgtcgtgatc gtcttcatgg agcgggcaca
      901 gcgccggatt ccgatccagt atccgaagcg acagatcggc aaccggatat tccagggtga
      961 cagctcgcac ttgccgctga agcttaactc atcgggcgtt attcccccta tcttcgcatc
     1021 gtcgctgctc ctgctgccga tcacggcggc gcagttcatg tctgggcagg gaccggaatg
     1081 gctcaacacg atcgttgcgg cgcttggtcg aggtcagccg ctgcacctca tcatctacgt
     1141 ggcgctgatc gtgttcttca cgttcttcta cacggccgtg gtgttcaatc cgcaggagac
     1201 cgcggagaat ctgcgtaagt acggcggttt catcctgggg tatcggccgg ggcagaagac
     1261 ggccgagtac atcgactacg tcctcacccg catcacggtg gtcggcgcca tctacttgtc
     1321 ggcggtttgc gtgctgccgg agttcctcat ctcctacgcg ggggtaccgt tctacttcgg
     1381 tggcacctca ctcttgatcg tggtgagcgt gacgatggac acggtggccc aggtgcagag
     1441 ccacctgctg gctcaccagt acgagggcct catcaagaag gcgaagctta ggggagcaac
     1501 gcgagggcgg cgatgaatct cattttgttg ggcccgccag gtgcaggcaa aggcacgcag
     1561 gccgcaacca tcgccgcttc tcgtgggctg atccagctgt cgacgggaga catgctgcgg
     1621 gcggcagtcc aggcgggcac ggagcttggc aagaaggtca agcccatcat ggaaagcggc
     1681 cagctggtcc cggacgagct ggtcatcgga atcatcgacg agcgcatcgc acagccggac
     1741 tgcgagaatg gcttcattct cgacggcttc ccgcgcaccc ttccacaggc ggcggccctc
     1801 gacgagctcc tggaaaagcg gaacaagcag ctcgacctcg tcatcgagct caaggtcgac
     1861 gatgcggtcc tgatcgagcg catatcaggc cggttcgcct gtgcgaagtg cggggcgggc
     1921 tatcatgaca agttcaagca gccgaagacc ccaggcgtct gcgacgcgtg cggctcaacg
     1981 gaattcgtcc gccgcgccga cgacaacgcc gagacggtga gaactcggct gatggcctac
     2041 tacaaggaaa ccgcgccgct gatcggctac tacttcgcca agggcaagct gaagtcgatc
     2101 aacggcatgg cacccatcga ggaggtgacg cgagacatcg agcgcctcct cgacgaagtc
     2161 gtggcggctt gacgcggggt gagcgcgttg acgcgaggca tgcgagcggt taaaaccacc
     2221 gcgattacat gccgcaccct ggcgagatcg gagctggtgt tggagcacca gacgccggtc
     2281 tttgctgtgt tgggcttcca atcacaaact tatcgcccgc tgaggcggta gcgggcggag
     2341 ctatcggcag accgcttgct cactcgagga gacacacgtg gcccgtatcg ctggcgttaa
     2401 cattccaacg caaaagcgcg tcgttatcgc gctgcagtac atacacggca tcggccccaa
     2461 gttcgcgaaa gagatctgtg aaaaggtggg cattccaccg gagcggcggg tgcacgagct
     2521 caccgatgcc gaggtgatgc agatccgcga gacgatcgat cgtgactacg tggtcgaggg
     2581 tgacctgcgc cgtgaggtgg cgatgaacat caagcggctg atggacctcg gctgctaccg
     2641 cggtctacgt caccgccgtg gtttgccggt ccgcggccag cgaacgcaca cgaacgcgcg
     2701 cacacgtaag ggcccggcca agccgatcgc cggcaagaag aaggcgtgat cggcctctcg
     2761 ggttgtgcag gcgcttgatt tttcaacaga actcaccagc tttcaggaac cgaacgactc
     2821 atggctagag agcaacaggc gcgcacgaag gtgcgccgca aggagcgcaa gaacatcagc
     2881 tccggcgtgg cgcacgtcaa cgcgaccttc aacaacacga tgatcaccat caccgatgcc
     2941 caaggcaata cgattgcttg gtcgtcggca ggtgcgatgg gtttcaaggg gtcgcggaag
     3001 tcgacgccgt acgcagctca ggttgccgct gaggatgccg gcagaaaggc catcgagcac
     3061 ggcatgaaaa ttctcgaggt ggaggtctct ggcccgggcg cggggcgcga gtcggcgctc
     3121 agggcgctgc aggctgtcgg cttccagttg accgcaattc gcgacgtaac gcccattccg
     3181 cataacggct gcaggccgcg caagcgtcgt cgcgtctaat cgatccgagg aatgctaact
     3241 ggcccga
//
LOCUS       DASUMG010000192         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1069118, whole genome shotgun sequence.
ACCESSION   DASUMG010000192 DASUMG010000000
VERSION     DASUMG010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069118"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(560..1339)
                     /locus_tag="VFX46_03165"
     CDS             complement(560..1339)
                     /locus_tag="VFX46_03165"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEX5599615.1"
                     /translation="MQLKGKTAIVTGSTSGIGLGIAHALAKEGANIVMNGLGDENENA
                     EQIRAVAAHGTRVMFHGANMLRHNQIADMVASAEQNFGSVDILVNNAGIQHVAPIEEF
                     PIEKWDAIIAINLSAAFHAIRAAIPGMKARRWGRIINICSAHSLTASPNKCAYVAAKH
                     GLAGLTKAVALEVARHGITCNAISPGFVWTPLVERQIPDLARTQGISEEQAREQILSR
                     QPTRQFITVEQVGALAAFLASDAAASITGANYSIDGGWTAE"
     gene            complement(1465..>2630)
                     /locus_tag="VFX46_03170"
     CDS             complement(1465..>2630)
                     /locus_tag="VFX46_03170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5599616.1"
                     /translation="GFWSAVVFATLPGVSLSAGIISTDVPLLFFWAVGLYAFVRLLDS
                     DRWWPAVLLGVALGLGFNAKYAMVYFLLSAAAYMVAVPEKRRLLRDARTYAALGIGLL
                     LIAPNLAWNAANSFATFAHTADNANWSGTLFHPEKALEFFGAQFGVFGPILFGSLLVI
                     TWRAWRDGLPEPERLLLAFALPVIVLVTVQAFLSRAHANWAAVSYVSATVLVTATMLR
                     EGAWRWFKGSLALHAAILLGIGIATATAGSFRLPVGGDPFARTLGWRAIAEETATVLA
                     EARQSGRPFGAVIADDRAITAELLYYMRDEPTSVFAWREPGVRPKDHYELTRAYQRGS
                     PEPVLLVSFRRGAERVTSRFAVVKPLLEREVPAGAGPGRRVAFYRLEGYAGGE"
BASE COUNT          526 a          852 c          785 g          467 t
ORIGIN      
        1 tgctgcggcc tgttccgccc gatccatctc aacctcatca tccttcacgc ttgcaagaaa
       61 gcgtcggcaa attcggctca aacaaaccgt cttgtgcaca agaaggcaaa tgctttcagc
      121 acggtttggc gcgccttggc caagctcggc atttgaacag ccaccagctc ttgcccctag
      181 ggcttcggct tcaatcgcga tcccgtgaac agtctcccca cgtttagcag tggctcacag
      241 gcggctccgg aggcataagt tttttcagaa atgcccctat catgtgttct tctgtcgcgc
      301 ggcatacctg tagctggcgc ggcctgctga tttggtcttg catggctcga accaggatct
      361 gacaactcgg ttcgcaatgg tgaaaaaccg gctgagacat cgttcacgtg aggtcgcgat
      421 tgcggtggct atcaccttgg ccgcagtggc ggcggtgatc gcaacattgt cgagccagga
      481 ggaggacgcc caaaccgcag agccagtcgc cctcatcacg cccaagaaat gagggcatta
      541 ggccgtgggg ccgctgccgt cactcggcgg tccatccacc gtcgatggaa tagttggcgc
      601 cggtgatcga ggcggcggcg tcgctggcca gaaatgcggc caaagcacca acctgctcta
      661 cggtgatgaa ctgccgcgtg ggctggcgtg acaggatctg ctcgcgcgcc tgttcctcgc
      721 tgatgccttg tgtgcgggca agatcgggaa tctgacgctc caccagcggt gtccagacaa
      781 agcccggcga tatcgcgttg catgttatac cgtgacgggc aacctcaaga gcgacagcct
      841 tcgtgagacc agccagcccg tgcttggctg cgacatatgc gcacttgttg ggtgatgcag
      901 ttagtgagtg cgctgagcag atgttgatga tccgccccca ccgccgcgcc ttcatgccgg
      961 gaatcgcggc gcggatcgcg tgaaaggccg cggacaagtt gattgcgatg atggcgtccc
     1021 acttctcgat ggggaactcc tcgatcgggg cgacatgctg aatgcccgcg ttgttgacga
     1081 ggatgtcgac gctgccgaag ttctgctcag ctgacgcaac catgtcagcg atctgattat
     1141 ggcgcaacat gttggccccg tggaacatga cccgcgtccc gtgagccgcc acggcccgga
     1201 tctgctcagc gttctcgttc tcgtccccga gcccgttcat gacgatgttc gctccctcct
     1261 tcgcgagagc gtgggcgatg ccgagcccga tgcccgatgt ggagccggtg acgatcgccg
     1321 tctttccttt gagttgcata gctgcctccc attcccgagg ttgtacgttt aaccgggccg
     1381 ctcgacgctg aaccaacgaa tgatccgcgt gcgcccgtca ttcatccggt gaacttgagg
     1441 gctccggcat agatcctgag cggcctactc gccgcctgcg tagccttcaa gcctgtaaaa
     1501 cgcgactctg cggcccgggc ccgcccccgc cggcacttcg cgctcaagga gcggcttcac
     1561 gacggcaaat ctgctggtta ccctttccgc tcccctgcgg aagctgacca agagcaccgg
     1621 ctccggcgag cctctctggt aggcacgagt cagctcgtag tgatccttgg ggcgtacacc
     1681 cggctcgcgc caggcaaaca ccgacgttgg ctcatcgcgc atgtaataga gcagctcggc
     1741 cgtgatcgcc cggtcgtccg caatcacggc gccgaacggc cggccggatt ggcgcgcttc
     1801 cgccagcacc gtggcagtct cctccgcaat agcccgccag cccagggtcc gcgcaaaggg
     1861 gtcgccgcca acaggcagac ggaaggagcc ggctgtcgcc gtcgcaatcc caatccctaa
     1921 gagaatcgct gcgtgcagcg cgagcgagcc cttgaaccaa cgccacgctc cctctcggag
     1981 catggtcgcc gttacgagca cagtggcaga cacgtagctc acagcggccc agttcgcatg
     2041 cgctcgcgaa agaaaggcct gcacggtaac gagtacaatc acaggcagcg caaacgccag
     2101 caacaaacgc tcgggctctg ggagcccgtc cctccaggcg cgccaagtga tcaccagcag
     2161 cgagccgaaa agaatcggcc caaacacgcc gaactgcgcg ccgaaaaact ccaacgcttt
     2221 ctcgggatga aagagcgttc cgctccagtt ggcgttgtcg gctgtatggg caaacgtggc
     2281 gaagctgttc gcggcgttcc atgcgaggtt cggcgcaatc agcagcaggc cgatgccgag
     2341 ggctgcatag gttcgggcat cacgaagcag gcggcgcttt tccggcaccg ccaccatgta
     2401 ggcggccgcc gaaagtaaga agtacaccat cgcgtacttc gcattgaacc ccaacccgag
     2461 cgccacaccc agcagcaccg cgggccacca gcgatcggaa tcgaggagcc gcacgaaggc
     2521 ataaagcccg acggcccaga agaacagcag cggcacgtcc gtcgagataa tgcccgcgga
     2581 caacgaaacg ccgggcagcg tcgcgaagac gacggcggac cagaacccca
//
LOCUS       DASUMG010000193         2824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_426569, whole genome shotgun sequence.
ACCESSION   DASUMG010000193 DASUMG010000000
VERSION     DASUMG010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426569"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFX46_03175"
     CDS             complement(<1..1148)
                     /locus_tag="VFX46_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX5599617.1"
                     /translation="MPKRAVSLDDKFDLNEPRQILTGTQAIVRLALMQQVRDKQAGLN
                     TAGYVTGYRGSPIAGLEGAFLRAQPILAKHNILFHPGLNEDLAATALWGSQQAELRGE
                     GKYDGVFGIWYGKGPGVDRTGDVFRHANHAGTSKHGGVLALLGDDHTCESSTSAHQSE
                     FAMVDYMIPVLNPAGVQEILDFGLLGFALSRYAGVWVALKCVKDNIESTATVDGRIDR
                     LKIVIPSEDEFRMPPGGLNIRLNDPPLAKEARLHDFKRPAMLAFARANKLDRIIMSGG
                     PSPKLGIITTGKSYLDVRQALDELGIDEVKADQLGIRLYKVGMVWPLEPRGVAAFADG
                     LETIIVVEEKRSLIETQVKEQLFDTPNRPRVIGKKDENEQWLFPAKGAL"
     gene            complement(1350..2393)
                     /locus_tag="VFX46_03180"
     CDS             complement(1350..2393)
                     /locus_tag="VFX46_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599618.1"
                     /translation="MDRRTFLKTTTVAAGTGLALSSTSALAQAEADALAAPAISRGTI
                     ELSFAAAWRPDVPVFGDAAHRIQQRLGQVLHDRYRIVPAGPGDEADLHFGPISADQHS
                     AFAFFAGLPGSHGLEPHSLQAWVAVGGGQMLWDDLAAQHGFKPFLAGHTGPNPGLWTN
                     RPLWSAADLSGEPIVLAGMGQHLAQKFGFQALNIHPRELGGALASGQIAAVEWGNAVA
                     TLVLCLPQSATRFYTGGIHRNGMALSLNVRRAAWERLDTAARLAIEDLSAREFSVAVA
                     ESLAHAQFAAEAIANMPDLEVAAFPPSISTAIDQAVTELIHEIATGSPDAARIRDSYL
                     AFRRHLAAPHLSS"
     gene            complement(2513..>2824)
                     /locus_tag="VFX46_03185"
     CDS             complement(2513..>2824)
                     /locus_tag="VFX46_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5599619.1"
                     /translation="ALNEEYIYGEDGRLQNPSFLDYRMPVASDLPMIDTVIIEVPNPR
                     HPYGVRGVGETPIVPPLAAIANAVENATGLRFTELPMSPPRVLAAIEAARARGDKNLK
                     V"
BASE COUNT          538 a          862 c          876 g          548 t
ORIGIN      
        1 agcgcgccct tggccgggaa gagccactgc tcgttttcat ccttcttgcc gatgacccgc
       61 ggacgattcg gcgtgtcgaa gagctgctct ttgacttgag tctcaatgag cgaccgcttt
      121 tcctcgacga cgatgatcgt ctcaagacca tcggcaaaag cagcaacacc acgcggctcg
      181 agcggccaaa ccattccgac cttgtacagc cgaattccaa gctggtcggc cttcacttcg
      241 tcgatgccga gctcatcgag ggcctggcgg acgtcgaggt agctcttacc cgtggtgatg
      301 atgcccagct ttggcgacgg gccgccgctc atgatgatgc ggtcgagctt attggcccgc
      361 gcaaatgcca gcattgcggg gcgcttgaag tcatgcagcc gcgcttcctt cgccaggggc
      421 gggtcgttaa gtcggatgtt gagcccgccc ggaggcatcc ggaactcatc ttcgctcggg
      481 atgacgatct tcagccgatc gattcgacca tcgactgtcg cggtgctttc gatgttgtcc
      541 ttgacgcact tcagcgcgac ccacacgccg gcataccgcg agagcgcgaa gcccagcaga
      601 ccgaaatcca gaatttcctg tacgccagcc gggttcagca cggggatcat gtagtccacc
      661 atcgcgaact cggactggtg cgcagaggtg gaactttcgc aggtgtgatc gtcgccaagc
      721 agcgctagca cgccgccgtg cttggatgtg ccggcatggt tggcatggcg gaagacgtcg
      781 ccggtgcggt cgacgccagg ccccttgccg taccagatcc cgaagacgcc atcgtatttg
      841 ccttcgcccc gcagctctgc ctgctgcgat ccccaaagcg cagtggctgc gaggtcctcg
      901 ttgaggccag gatggaagag gatgttgtgc ttcgccagaa tgggctgcgc ccggagaaag
      961 gcgccttcga ggcctgcgat cggggaaccg cgatagccgg tgacgtatcc cgcggtgttc
     1021 agacccgcct gcttgtcccg gacctgttgc atcagcgcga gccggacgat ggcctgggta
     1081 cctgtcagga tctggcgcgg ttcgttgagg tcgaacttgt cgtccagact gacggcgcgc
     1141 ttcggcatgg gacacctcgg cattaggctc gacgctgctc gcgtaaagag ccggtccgac
     1201 taggtcagca ttgctgacat aatccatacg cgagaaacgc cgaatcagca aacgttggca
     1261 gggtgccggc gttctctttc cagaatgttg gttgcgaatg acctgggtgc aaccaaatgg
     1321 gtgtgcgctg caacgtaagc gcgcatgcct cagctcgaga gatggggagc agccaaatgc
     1381 ctgcggaatg ccaagtagct gtcgcgaatg cgggccgcat ccggtgagcc ggtcgcaatc
     1441 tcgtgaatga gttccgtcac agcttggtcg attgctgtgg agattgatgg aggaaaggcg
     1501 gcgacctcca gatctggcat gttggcaatc gcctccgcgg caaattgtgc atgcgcaagc
     1561 gattcggcca ctgccactga aaactcccgc gccgacaaat cctctatggc gaggcgggca
     1621 gcagtgtcca agcgctccca ggcggcgcga cgcacattga gcgataaggc catgccattt
     1681 cgatggatgc cgccggtgta gaagcgggtt gctgattggg gcaggcaaag aacgagggtg
     1741 gccaccgcat tcccccattc caccgcagca atctgcccgc tcgctagcgc gccaccgagt
     1801 tcccgcgggt ggatattcag ggcctgaaat ccgaactttt gtgcaagatg ttggcccatt
     1861 ccggccagca cgataggctc gcccgacaaa tcggcggcac tccacagtgg acgatttgtc
     1921 cacaacccgg ggttcgggcc cgtatgtccc gcgagaaaag gtttgaagcc atgctgcgcc
     1981 gccaaatcgt cccagagcat ctggccgccg ccgacagcga cccaggcttg gagactgtga
     2041 ggctccaggc catgcgatcc cggcagtccg gcgaagaaag cgaaggctga gtgctgatcc
     2101 gcggaaatgg ggccgaaatg gaggtctgcc tcatccccag gtccggccgg aacaatccga
     2161 tatcgatcgt gcaggacctg ccctagtctc tgctgaatgc ggtgagcggc atcgccgaaa
     2221 accggcacgt ctggcctcca cgctgccgca aacgacaact cgatcgtgcc gcgcgaaatc
     2281 gctggcgccg ccagtgcatc agcttcggcc tgggcgagtg cggaggtaga cgacagtgcg
     2341 aggcctgtgc cggccgccac ggtcgtcgtt ttcagaaatg ttctgcggtc catgccagcg
     2401 cctccccact gcccgactgc ctgagccttc gccggatgaa acccgaatgg ctgtcgtctg
     2461 acaatgcccc ttgcgaggag gtttcacacg ggagtgggtg agccgccatg catcagacct
     2521 tcaaattctt gtctccgcgg gctcgcgctg cctcgatagc tgccaacacc ctcggtggcg
     2581 acatcggcag ttccgtgaaa cgcaatcccg tggcgttctc aactgcgttc gcgatggctg
     2641 ccagaggtgg tacgatcggt gtctcgccca ctccccgcac accataggga tgccttgggt
     2701 tcggcacctc gatgatgacc gtgtcgatca tgggcagatc cgatgcaacg ggcatgcggt
     2761 agtcgaggaa gctcggattc tgcaaacgtc cgtcttcgcc atagatgtat tcctcgttga
     2821 gggc
//
LOCUS       DASUMG010000194         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_25368, whole genome shotgun sequence.
ACCESSION   DASUMG010000194 DASUMG010000000
VERSION     DASUMG010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25368"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1893
                     /locus_tag="VFX46_03190"
     CDS             178..1893
                     /locus_tag="VFX46_03190"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HEX5599620.1"
                     /translation="MSPEAKIVLRRRYLIKNHHGTVTETIPQLFWRIATDVARAEQLY
                     PGPTRLAGAAQRFYESMARLEFLPNSPTLMNAGRPLQQLAACFVLPVEDSLNSILDAV
                     KQQALIHQSGGGTGFSFSRLRPHDDLVSSTHGIASGPVSFMRIFNMATDVIKQGGTRR
                     GANMGILRVDHPDILEFIGLKQNPAEMTNFNLSVGLTADFMRAFERRQRYGLINPRTG
                     KAARRLPAQMVFDRLAQAAWATGEPGVIYLDRINAANPTPRLGDIEATNPCGEQPLLP
                     YESCTLGSIDVSKFVARTGGTAAIDYARLDETISLAVRFLDNVIDRNHYPFKGIETAT
                     LQTRKIGLGIMGFADLLIALDIPYGTDAAIAQATSLMSFVQTRAHDASRQLALQRGLF
                     PAYQGSRLDARRERRRNATVTTIAPTGTISLIAGCSPGIEPLYGVRILRRALEDLELT
                     MVHPAFAKMIRAHGLPLEELRTDLAASPSIQDIARIPREIRRLFITAHDVPPQQHVRM
                     QAVFQRFSDSGVSKTINMPSSATKADVADAFLLAYELGCKGLTVFRSGSRENQVLSCS
                     PAQPC"
     gene            complement(2271..2429)
                     /locus_tag="VFX46_03195"
     CDS             complement(2271..2429)
                     /locus_tag="VFX46_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599621.1"
                     /translation="MSTLQALIFDFDGVIANTEPLQERSQKEASARLEYLKEWVRKRP
                     EVLPELWT"
     gene            complement(2520..2732)
                     /locus_tag="VFX46_03200"
     CDS             complement(2520..2732)
                     /locus_tag="VFX46_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599622.1"
                     /translation="MTAIGNPTAPGETIPLSLDPAGAKPGPVVRPGVTLSVLDGDLQP
                     SGWVYSVRTDDGVKGWIPEKRLRLKF"
BASE COUNT          593 a          929 c          823 g          545 t
ORIGIN      
        1 gagttcttcg cggtacgagc gcatagtcct gtcaccagca ctgcattgtg agaccgggag
       61 ccgaaaagat tcaagcccgg caatatcgtc aaacggcgga gcgggcggct ataataccgc
      121 atgcgtgctc cccgctctgt ccggtcgcct gccccccctt ccaggcccgt cccgcgattg
      181 tctcccgagg cgaagatcgt gcttcggcgg cgatacctca tcaaaaatca ccacggcacg
      241 gtcacggaaa cgatcccgca attgttctgg cgcattgcca ctgacgtcgc acgagccgaa
      301 cagctctatc ccggcccgac gcgccttgcc ggcgccgcgc aacggtttta cgaatccatg
      361 gcacgcctgg aatttctccc caattcgccg acgctcatga acgccggacg tcccctgcaa
      421 caattagctg cctgcttcgt cctgccggtc gaagactcgc tgaattccat cctcgatgca
      481 gtgaagcagc aggccctgat ccatcaatcg gggggtggga cggggttttc gttcagccgg
      541 cttcgccccc acgacgacct cgtgtcctcc acgcacggca tcgcttccgg tccggtgtcg
      601 ttcatgcgca ttttcaacat ggccacggac gtcatcaaac aaggtggcac acggcgcgga
      661 gccaacatgg gcattttgcg tgtggatcat cccgacattc tagaattcat cgggctgaaa
      721 cagaatccgg ccgaaatgac caacttcaat ctatcggtcg ggcttacagc agacttcatg
      781 cgtgccttcg aacgtcggca gcgctacggc ctcatcaatc cgcgcaccgg aaaagcggcg
      841 aggcggctgc cggcccagat ggtgttcgat cgcctggcgc aggccgcctg ggccaccgga
      901 gagccaggcg tcatctatct ggaccggatt aacgcggcca atccgacgcc ccggctaggc
      961 gacatcgaag ccacgaaccc ctgcggcgaa caaccgctgc tgccgtacga atcctgcacg
     1021 ctgggatcca tcgatgtctc aaagttcgtc gcgcgtaccg gcggcaccgc agccatcgat
     1081 tacgcgaggc tcgatgaaac aatcagcttg gccgtccgct ttctggacaa tgtgatcgat
     1141 cgcaaccact acccgttcaa gggcatcgaa accgccacgc tgcagacgcg aaagatcgga
     1201 ctcgggatca tgggattcgc cgatttgttg attgccctgg acatccccta tggcaccgac
     1261 gccgccattg cccaggcgac ctcgctgatg tcgtttgtcc agacgcgtgc ccacgacgcg
     1321 tcccgccagc ttgccttaca gcgagggcta ttcccggcct accagggcag ccggcttgac
     1381 gcccgccgcg agcgacggcg caatgccacc gtcaccacca tcgcgccgac gggaacgatc
     1441 agtctcatcg caggctgctc acccggcatt gaaccgctgt atggcgtgcg cattctccgc
     1501 cgggctctgg aagacctgga gttgacgatg gtacacccgg cttttgcgaa gatgatccgc
     1561 gcccacggcc ttcctctgga agagcttcgg actgatctgg cagccagccc gtccatccag
     1621 gatatcgcac gtattccgcg agagattcgg cggctcttta tcaccgcaca cgacgtcccg
     1681 ccacagcaac acgttcgtat gcaggccgtc tttcagcgct ttagcgacag cggcgtgtca
     1741 aaaacgatca atatgccatc gtccgccacc aaggcagacg tggccgatgc ctttctcctg
     1801 gcctatgaac tggggtgtaa gggtttgacg gtcttccgta gcggcagccg tgaaaatcag
     1861 gtcctgtcct gctctcccgc acaaccgtgc tgatcccgac tcgcggcata atcaaagggg
     1921 aaattgactc tcagagggga tggtgataag agaaggttca atcgtccagc ttggaggtat
     1981 gtcgatggcc cgtcaaccgg agccggactt ggaatttacg gaagaggaac tggatcagac
     2041 gactgccacc agaccttcct ctcccatgaa gtccggaaag aaatcgcgtg gcggacgacc
     2101 gctgctgtgg atttttttgc tcctcctcgt cgcaggaatc ggctacctcg ccatggaacc
     2161 ggaacaactc gcggactggc tggctccctt tacgggtgac aatccgcctg aaccgcacgc
     2221 gacggcgaca agacccaaga ctgctgcacc gacggctcct ccacccgctt ctacgtccac
     2281 agttccggca acacctccgg ccgcttccga acccactcct tcaggtactc caaccgagct
     2341 gatgcctcct tctgcgaccg ctcctgcaac ggctctgtgt tggcaatgac gccgtcgaaa
     2401 tcgaaaatga gcgcttgcag tgtcgacata gaaacgacgg gaagatcatg ttggaagtga
     2461 cggcgcggat tatatcacag gggtcacaaa aagaaggcgg accaacgcag tcgaaggatt
     2521 cagaacttca gtcgcagccg cttttctggg atccagccct tgacgccatc gtccgtccga
     2581 accgagtaga cccagccgct cggttggagg tcgccgtcga gcacgctcaa tgtgacgccg
     2641 ggacggacca caggacccgg cttcgcaccg gcagggtcca aagagagcgg aattgtttcg
     2701 cccggagcgg tcggattccc gatcgcagtc accttctgcc cttcggcaaa catcggagaa
     2761 gcgacagcgg atgcgggtgc tgtcggagcg gtcgcagaag gaggcattgc agaaggagtc
     2821 gcgggagcac ccatcgggtc ggtcggagtt cctgaaggag cggtttctga agcggtcggt
     2881 ggcgttgccg
//
LOCUS       DASUMG010000195         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_266282, whole genome shotgun sequence.
ACCESSION   DASUMG010000195 DASUMG010000000
VERSION     DASUMG010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266282"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VFX46_03205"
     CDS             <1..1095
                     /locus_tag="VFX46_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599623.1"
                     /translation="VSITVEASDKVQGANFLIEKAPRATTNGQRQPERSPVSLDWGFQ
                     GVEEEAEETGHEEEQARAEEVETRRSRRRRRRRGRRGGARGEHVQATPAVPGNGADGE
                     LMYAHGEHDLSDHEHDISEEDEEEEAVDEAVEEVSFSPDEEPAEAVASADDVEDGEEE
                     VQVAESSEEPKDEQRRHRRGRRRGRRGGRRDRDGVAPAVAGESGVVEANAEAVEPESG
                     PVLEASSEAPVQLDATADQTVTSAPGSEDVVPGAEVAIAEPESGAVAHAEAESGPAMT
                     EQAGAEPAQASTAPPVEASVPALESQAEGASQEQRPSRTPRTEPVASEPIIERIVLGP
                     EIGAVTPERPTPSEQPARRGWWQRRFGVNG"
     gene            complement(1201..1878)
                     /gene="aat"
                     /locus_tag="VFX46_03210"
     CDS             complement(1201..1878)
                     /gene="aat"
                     /locus_tag="VFX46_03210"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEX5599624.1"
                     /translation="MTARDSPLLEITPQVLLKAYTCGIFPMAESADDPQLYWIEPQAR
                     GVLPLDNVRVPKRLRRTIRQKPYQVRIDTDFAGVIAGCAAPKPGRRTTWINDRIRKLY
                     RELHRLGYCHTVEVWDGERLVGGLYGVALGGAFFGESMFSEVRDASKIALIYLCARLI
                     YGGFVLLDTQFVTDHLRQFGTIEVDRAEFHRQLEAALKINGDFFRLEPEPTAERVLDI
                     VLGNQAD"
     gene            complement(2035..>2478)
                     /locus_tag="VFX46_03215"
     CDS             complement(2035..>2478)
                     /locus_tag="VFX46_03215"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195221.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX5599625.1"
                     /translation="MITGVDLVIEQIRVAADRPLSLKQEEVTFSGHAIECRINAENPK
                     TFRPSPGKITYWHPPGGLGVRVDSGVYQGYKIPPYYDSLIGKLIVHGKTRNECLMRLK
                     RCLSEFVIDGIETTIPLFADLVRHPDIVNGAYDIHWLEKYLSKAE"
BASE COUNT          486 a          726 c          849 g          417 t
ORIGIN      
        1 gtctcgatca ccgtcgaggc gagtgacaag gtgcagggcg ccaacttcct gatcgagaag
       61 gcgccgcgcg caaccaccaa cgggcaacgg cagccggagc ggagcccggt cagcctggac
      121 tggggcttcc agggcgtcga ggaggaggcc gaggaaacag gacacgagga agagcaggca
      181 cgggcggagg aagtcgaaac gcggagatcg cgccggcggc gccgtcgtcg cgggcgtcgt
      241 ggtggtgcac gtggcgagca cgtgcaggcg acaccggcgg tgcctggtaa cggcgccgac
      301 ggtgagctga tgtacgcgca cggcgagcac gacctttcgg accacgagca cgacatctcg
      361 gaggaggacg aggaagagga ggccgtcgac gaggccgtcg aggaggtttc cttctcgcct
      421 gatgaggagc ctgcagaggc ggtcgcgtct gccgacgatg tcgaggacgg cgaggaagag
      481 gtgcaggtcg ccgaaagctc cgaagagccg aaagacgagc aacgtcgcca tagacgaggc
      541 cggcgccgcg gtcgccgggg cggccgccgg gaccgggatg gtgtcgcgcc tgctgttgcc
      601 ggtgagtccg gtgtcgtcga ggccaatgcc gaggcggtcg agcctgagag cgggcccgtg
      661 cttgaggcaa gctccgaggc gcctgtgcag ctcgatgcta ccgctgatca gacagtaaca
      721 tcggctcccg gctcagaaga tgtggtgccg ggcgccgaag tggcgattgc cgaaccggag
      781 agcggggctg tcgcccatgc cgaggcggag agtggtcctg ctatgaccga acaggcagga
      841 gcagagcctg cgcaggcttc tacagcacca ccagttgagg cctcagtgcc ggctttggag
      901 tcgcaggcag aaggggcctc tcaggagcaa aggccatcgc gcaccccgcg aacggagcca
      961 gtcgcgagcg agcccatcat tgaaagaatc gttctcggtc ctgaaatcgg cgcggtcaca
     1021 cctgagcgac caacgccttc cgagcagccg gcgcgccggg ggtggtggca gcgccgcttt
     1081 ggggtcaacg gttaggcttg ccagtcgggc gctcagctac attcgctcat tactgagcgc
     1141 tcgacgaaat cgcgctgaat agcggacgaa caggttcggc ggaagttggg gcagtctgaa
     1201 ttagtccgcc tgattgccga gcacgatatc gagcacccgc tctgccgtgg gttcaggctc
     1261 caggcggaag aagtctccgt tgatcttgag ggcggcttcg agctgacggt ggaattcggc
     1321 ccgatcaacc tcgatggtgc cgaactggcg caggtggtcg gtcacgaatt gcgtatcgag
     1381 caacacgaag ccgccgtaga tcaggcgggc gcacagataa atgagcgcga tcttggaggc
     1441 atcgcgaacc tccgagaaca tgctctcacc aaaaaaggcg ccgcccagag cgaccccgta
     1501 aaggccacca acaaggcgct cgccgtccca aacctcgaca gtgtggcagt agccgagcct
     1561 gtgcagctcg cgatagagct tgcggatgcg gtcgttgatc caggtcgtac ggcgtcccgg
     1621 cttcggagct gcgcaaccgg cgatcactcc ggcgaaatcg gtatcgatgc gcacctggta
     1681 cggtttctgt cgaatggtgc gccgcaggcg tttcggcact cggacgttat cgagcggaag
     1741 aacaccgcgc gcctgcggct cgatccagta gagctgcggg tcgtccgcgc tctcggccat
     1801 ggggaagatg ccgcacgtgt aggccttcag caggacctgc ggcgtgatct caagcagtgg
     1861 actatcgcga gcagtcatcg gattggtcgc ccggggcttc ggctggaacg ccggcgttca
     1921 agctaaatcg gccgagttct cccccagttc ccaggccgca atcgtctcag caatattatt
     1981 gctcaacctt gatcggccga gcaatatcaa tagccgcagg gcggaaggcg gcatttactc
     2041 agccttgctg aggtacttct ccagccagtg gatgtcgtaa gccccgttaa caatatcggg
     2101 atgccgcacc agatcggcaa acagcggtat cgtcgtctcg atcccgtcta tgacgaactc
     2161 cgacaggcag cgcttcagcc gcatcaggca ttcgttccgg gtcttaccgt ggacgatgag
     2221 cttgccgata agactgtcgt agtacggagg aatcttgtag ccctgataca caccgctgtc
     2281 gacgcgcacg ccaagcccac cgggcggatg ccagtaagtt atcttgccgg gtgatggccg
     2341 gaaggttttt gggttttccg cgttgatgcg gcattcgatg gcgtggcccg agaaggtcac
     2401 ctcctcctgc ttcaggctga gtgggcggtc tgccgctacg cggatttgct cgatgacgag
     2461 gtcgaccccg gtgatcat
//
LOCUS       DASUMG010000196         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_426810, whole genome shotgun sequence.
ACCESSION   DASUMG010000196 DASUMG010000000
VERSION     DASUMG010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426810"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFX46_03220"
     CDS             complement(<1..708)
                     /locus_tag="VFX46_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HEX5599626.1"
                     /translation="MAEITLTFPDGAKRKFPKGITGKELAETISKSLAKKAVAMSVNG
                     RLADLVDPIETDAEVKILTRNDPEALELIRHDAAHVLAEAVQELFPGTQVTIGPVIEN
                     GFYYDFYREEPFHPEDLLKIEARMREIIKRNKPFEKEVWSREKAKAYFREKGEEFKVE
                     LVDAIPENEEVKIYRQGEWLDLCRGPHMTATGQIGNAFKLQKIAGSYWRGDSSKAQLQ
                     RIYGTAWANEEDLQAYLK"
     gene            complement(868..1209)
                     /locus_tag="VFX46_03225"
     CDS             complement(868..1209)
                     /locus_tag="VFX46_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEX5599627.1"
                     /translation="MALRTGGRSGILNFDSVLSAIGRPYHGYHRTIHEKAAALLEGVA
                     TCHGFVDGNKRTALILTLLMIEESGYRLEPANSTEDLDVALEEFVLGVVAHKYTFEQM
                     VAWFKARIRRK"
     gene            complement(1407..1748)
                     /gene="yidD"
                     /locus_tag="VFX46_03230"
     CDS             complement(1407..1748)
                     /gene="yidD"
                     /locus_tag="VFX46_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX5599628.1"
                     /translation="MSVRPLLGAALKLPIHLYRWTLKPFVGWNCRHLPTCSEYALEAI
                     DKNGTWRGFWLMVSRLSRCHRFGTAGFDPVPDIRNERHPFAPWRYGRWTGRHMTQHWT
                     EERRTSYGQEN"
     gene            complement(1899..>2115)
                     /locus_tag="VFX46_03235"
     CDS             complement(1899..>2115)
                     /locus_tag="VFX46_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX5599629.1"
                     /translation="ELRDVGRTMRRMLKENGPPPTGRWADLGILEPVRDYKARHASTL
                     LVFDAVERALDEIEARRSGTDQAVAAQ"
BASE COUNT          395 a          696 c          575 g          449 t
ORIGIN      
        1 cttgaggtag gcttggaggt cctcctcatt ggcccaggcg gtgccgtaga tgcgttgcag
       61 ctgcgccttg ctggaatcgc cccgccaata gctgcccgcg atcttctgca gcttgaacgc
      121 attgccgatc tgacccgtcg cggtcatatg cgggccacgg cagaggtcga gccattcgcc
      181 ctgccgatag atcttgacct cctcgttctc cggaatggcg tcgacgagct caaccttgaa
      241 ctcctcgccc ttctcgcgga agtaggcttt ggccttctcg cggctccaga cctccttctc
      301 gaacggcttg ttgcgcttga tgatctcgcg catgcgcgct tcgatcttca gcaaatcctc
      361 cggatggaag ggctcctcgc ggtagaaatc gtagtagaag ccgttctcga tgaccggccc
      421 aatggtcacc tgggtgcccg ggaacagctc ctgcacagcc tccgccaaaa cgtgcgcagc
      481 gtcatgccgg atgagctcca gcgcttccgg atcgttacgg gtaaggatct ttacctccgc
      541 gtccgtctcg attggatcaa cgagatccgc gagccggccg ttgacgctca tcgcgacggc
      601 cttcttggcg agcgacttgg agatcgtctc ggccagctcc ttgcccgtga tccccttcgg
      661 gaatttccgc ttcgccccat ccgggaaggt cagtgtaatc tcggccataa ttcgcgatct
      721 cctctcactc gctgcatacc acgcaggtaa gacaaaccgg gcctggcaca ttttcacccg
      781 cgccgcggcc cggtgcggcg ctgttgcaga caactcgctg gcggaagtca agcagtttgt
      841 aactattggg tgtcagcgcc gctcgctcta cttgcgacgg atgcgcgctt tgaaccaggc
      901 gaccatctgc tcgaacgtgt acttatgggc gacgacgccg aggacaaatt cctccagcgc
      961 gacatccaag tcttcggtgg agttggccgg ctccaatcga tacccgctct cttcaatcat
     1021 cagcaaagtc agaataagcg cggtacgctt gtttccgtcg acgaaaccgt gacaagtcgc
     1081 gacaccttca agtaaagcgg ctgccttttc gtgaatagtc cgatggtagc cgtgatacgg
     1141 ccgaccaatc gccgacaaga cactgtcgaa attcagaatg cctgaccgcc cgcccgttcg
     1201 taacgccacc tcatgggcgc gatatacatc aacaacggta atccggtagt gccgcctcct
     1261 acttgtcggc aaggcgcttc agaacccttt tgtgccgctc agcaatccta tcaatcgttt
     1321 gtgctgatcg cgaccgcagg gtcttggtca cgctggtaaa cttccccgac gtagccgatc
     1381 ggccgacctt tgcggcagcg ggttttttaa ttttcttgtc cataggacgt ccttctctct
     1441 tccgtccaat gctgcgtcat gtgccgcccc gtccagcgac cataccgcca tggcgcaaac
     1501 gggtggcgtt cgtttcgaat gtccggcacg ggatcgaagc cggcggtgcc aaagcgatgg
     1561 cagcggctga ggcgcgaaac catcagccaa aatccgcgcc acgtgccatt cttgtcgatg
     1621 gcttcaagcg cgtactcgga gcacgtgggc agatgccggc aattccagcc gacgaacggc
     1681 ttcagggtcc agcggtagag gtgaatgggc agctttagcg cggcgccaag cagtggtctc
     1741 acagacaccc tttcgcgatc cgcccttacg tcgtcaagtc tagaactcaa tccgatccca
     1801 cactcaagtc attccgggcc ttgtgcccgg aatccagctc accacaatcg ccgagctatc
     1861 ggccgagtgg atcccggcga caagctgacg gctttcgatt actgcgccgc tacagcctga
     1921 tccgtccccg atcgccgcgc ctcgatctcg tccagcgccc gttcgacggc atcgaagacg
     1981 agcaacgtgg acgcatgccg tgctttatag tccctcaccg gctccagaat gccgagatcc
     2041 gcccaccgcc ccgtcggagg cgggccattc tccttcagca tccggcgcat cgtccggccc
     2101 acatcgcgca gctcc
//
LOCUS       DASUMG010000197         2614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_347326, whole genome shotgun sequence.
ACCESSION   DASUMG010000197 DASUMG010000000
VERSION     DASUMG010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347326"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VFX46_03240"
     CDS             complement(<1..1269)
                     /locus_tag="VFX46_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5599630.1"
                     /translation="MSTATLVKAVPQTDSAKPLTENSLAVSEPSHALARFATNLRHQD
                     IPESVRSRARHHALDAIGIALASTKFDFAHRTLNAIAGLGGSGPVPVIGMPARLSPRD
                     AAIVNGLLCHGLDFDDTHLDGVVHPTASAFPAALSAAMHVGATGRDLLTAYIVGVEVT
                     SRLGAVVKGAFHQVGFHPTGLVGVFGCALTAGVLFGLSERELVHAQGIALSLASGSLE
                     FLEDGAWNKRIHPGWAAAAGITAAALAKQGFKGASNPYVGRFGLYKSHLGPLAEGCDY
                     SLATAGLGETWEIARTSIKPFPACHFTHGCIDAALKLKKDGLDVSAIEKIEAFVPAEV
                     VKTVCEPEANKKRPANSYDAQFSIPFLVAAALVRGRMTLAEIEDVALSDPQILALADK
                     VQYRIDPNSPFPRAYSGEVRIALKDGKQVAA"
     gene            complement(1361..1849)
                     /locus_tag="VFX46_03245"
     CDS             complement(1361..1849)
                     /locus_tag="VFX46_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5599631.1"
                     /translation="MSERRTIVASYGRYLEDFVVGDVYEHRPGRTITEADNIQFSLLT
                     MNFHPMHCDAAFASKSEFGRPLVNSGLTLAIVLGMTVNDVSFKAIANLGWKDIKLIAP
                     VFPGDTIYARSEVLEVRESKSRPTQGIVTTRTEGYKADGTVFMEFIRMSLIPKRGHGV
                     GD"
     gene            2202..>2614
                     /locus_tag="VFX46_03250"
     CDS             2202..>2614
                     /locus_tag="VFX46_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599632.1"
                     /translation="MTEPTKGGLPLAVSAAIAIQALTSAAVLALTPIAPIVADAMGLP
                     AHVIGYQVSAVYAFAALISIFSGRIVRKYGPARSSQLALFAAAVGLAGFASGSAGIVL
                     IGSVLIGSGYALTNPAASELLIRLAPPDKRNLIFSL"
BASE COUNT          503 a          806 c          790 g          515 t
ORIGIN      
        1 cgcggcgact tgcttgccgt ccttcaaggc aatccgcacc tcgccagagt aggctctcgg
       61 gaacggcgaa ttgggatcga tccggtactg gaccttgtca gcgagggcga gaatctgagg
      121 atcggaaagt gccacgtcct cgatctcggc aagagtcatt cggccacgca ccaaggcggc
      181 ggcgacgagg aacgggatcg agaactgcgc atcatagcta tttgccggcc gcttcttgtt
      241 cgcttccggc tcgcagacag ttttcaccac ctcggcaggg acgaaagcct cgattttctc
      301 gatcgccgaa acatcgagcc cgtccttctt cagcttcagg gcggcgtcaa tgcagccatg
      361 ggtgaagtgg caagccggaa acggcttgat cgaagtgcgc gcgatttccc aggtctcgcc
      421 gaggccggcg gtcgcgagcg agtagtcgca accctcagcg agtggcccca ggtgcgattt
      481 gtagaggccg aaacgtccga cgtaaggatt gctcgctccc ttgaagccct gcttggcaag
      541 tgcagccgcg gtgatgcccg ctgcagcggc ccagccagga tggatccgtt tgttccacgc
      601 accatcctcg aggaattcca ggctcccgga ggcgagggac agagcgatgc cctgcgcgtg
      661 cacgagctcg cgttctgaga ggccaaataa tacgccggcc gtgagcgcac agccgaaaac
      721 gccgacgagc ccggtcgggt ggaagccgac ctgatggaag gcgcccttca caaccgcgcc
      781 gagccgggac gtgacttcga caccgacgat gtatgccgta agcagatccc ggccggttgc
      841 gccgacgtgc atcgccgccg aaagcgcagc cggaaacgca ctcgccgtcg gatgaaccac
      901 tccgtcgaga tgagtgtcgt cgaaatcgag gccgtggcag aggagcccat ttacgatagc
      961 agcatcgcgc ggcgagagcc gggcgggcat gccaatgacg ggaaccggcc ctgaaccgcc
     1021 gaggcccgcg atggcgttca aagtccggtg agcgaagtcg aacttggtgg acgcaagcgc
     1081 gattccgatc gcgtcaagcg cgtggtggcg cgcgcgagac cgcacactct caggaatgtc
     1141 ctggtggcgc agattggtgg caaatcgcgc aagcgcatgc gaaggctcgc tcacagccag
     1201 tgaattttcg gtgagaggct tcgcgctatc ggtctggggg acagccttca ctagcgtagc
     1261 ggtgctcatg ggttcctccc tgaatttctc gctgcggatc gacggcgacg cggacttaaa
     1321 ttctcgttct ctcggatgct ctcggcatcc gagaggctgg tcagtcccca actccgtggc
     1381 ccctcttggg tatgaggctc atccggatga actccatgaa tacggtaccg tccgccttat
     1441 atccttcagt tctcgtcgta acgatacctt gcgtaggacg cgatttgctc tcgcgcacct
     1501 cgagaacttc tgagcgagca taaatcgtgt cacccgggaa aaccggggcg atcagcttga
     1561 tgtccttcca gccgagattg gcgatcgcct tgaagctcac atcgttgacc gtcattccga
     1621 gcacgatcgc gagcgtcagt ccagagttga cgagcggccg tccgaactcc gacttgcttg
     1681 cgaaggcggc atcgcaatgc atcgggtgaa agttcatcgt gagcaggctg aattggatgt
     1741 tgtcggcttc tgttattgtc ctgcccggtc gatgctcgta aacgtctcca accacgaaat
     1801 cttcgagata ccgaccgtag ctggcaacga tcgtccgacg ctcgctcatc gtgccccttt
     1861 ccgaacctga caacgccgga aacggcgcca aacctgtgga caggttccgt atgtaaaagt
     1921 acgtacatgt tcggtcaagg tatctttcgc ggctgataga ggaatgtctt cgagctaacc
     1981 gtggacaagt cgatcacaac cgaagccgtc tcttgttggt tggtgcgcag cgtgcagccg
     2041 atgcatctcg caatggccgt cgcgaagact cggagcgtac tagtggacta cccagcagcc
     2101 ggcagcacgg cttgtctgct ggacgagcgc cttgtcgaac gatcaacaat gaggcgcaat
     2161 ccacgcgcct cagaccgcgc acacatcatg gaagcgatcc agtgaccgaa ccgacaaaag
     2221 gcggcctgcc gcttgcagtt tcggcggcta ttgcgatcca ggccctcacg agtgccgctg
     2281 ttctcgcgct tactccgatc gcgccgattg ttgccgatgc gatgggcctg ccggcgcacg
     2341 tcatcgggta ccaggtgagt gccgtctacg cgttcgcggc gttgatctcg atcttctccg
     2401 gccgcatcgt ccgcaagtac ggcccggcgc gctccagcca gctcgctctt ttcgccgcgg
     2461 cggttggcct tgccggtttc gcttccggaa gcgctggcat cgtgctgatc gggtcggtcc
     2521 taatcgggtc aggctacgcc ctgaccaacc ctgcggcctc cgagctgctc attcgccttg
     2581 cacctcccga taagcgcaac ctgattttct ccct
//
LOCUS       DASUMG010000198         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_828874, whole genome shotgun sequence.
ACCESSION   DASUMG010000198 DASUMG010000000
VERSION     DASUMG010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828874"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..714)
                     /locus_tag="VFX46_03255"
     CDS             complement(208..714)
                     /locus_tag="VFX46_03255"
                     /inference="COORDINATES: protein motif:HMM:NF042492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6653 family protein"
                     /protein_id="HEX5599633.1"
                     /translation="MTFERRLAGLFRMDDDTWARHANPWSVWTRVISLPLLMLAVWSR
                     VWIGWWSLLPIAGMILWLWINPRAFPPPRSTNNWASKGVLGERVWLNRREVPVPEHHR
                     LVPNILSAFSAVGAVFFVWGLVWLEIWPALLGLVLVMICKLWFVDRMVWLYEDMKDQH
                     PTYREWLR"
     gene            complement(743..>2673)
                     /locus_tag="VFX46_03260"
     CDS             complement(743..>2673)
                     /locus_tag="VFX46_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX5599634.1"
                     /translation="YSDADRDALHVEMADEAVHIGPAPAAQSYLVIENIIEACKKTGA
                     EAVHPGYGFLSEREAFPLALAEAGIVFIGPNPKAIAAMGDKIESKRAAAKAKVSTVPG
                     YLGEIADSAEAARIAEEIGYPVMIKASAGGGGKGMRIAHTRRELEEEFDAARSEAKAS
                     FGDDRVFIEKFIVNPRHIEIQVLGDKHGNVIHLGERECSIQRRNQKVIEEAPSPLLDE
                     KTRKAMGEQAVALAKAVGYDSAGTVEFVAGQDKSFYFLEMNTRLQVEHPVTEMITGID
                     LVEQMIRIAAGEELRLRQEDVTFTGWSVESRIYAEDPFRNFLPSTGRLVKYRPPVEGS
                     AEGMAVRIDTGVAEGGEISMYYDPMIAKLITHAPTREAAIELQAQALDAFYIEGIKHN
                     IPFLTALMQHPRWREGDLSTTFIQEEYPNGFEARAPGGEELQILAAVAAAIDHLHNCR
                     RRKITGQMPGRAVSFAKRRVVDVGNGVRRRVIVDGELDGPITVTLLDDDGNPSDTLEI
                     ASKWWPGEPVWSGMVRDRDVSVQVRPILNGFELAYRGMRVPARVYTEREAELAALMPV
                     KAAADTSKVLLCPMPGLVKAIHVEAGQEVKAGDPLAVVEAMKMENTLRAERDGIVKAV
                     NAKPGDSLAVDAVIMEFA"
BASE COUNT          501 a          884 c          805 g          483 t
ORIGIN      
        1 tcgatggccg cctgcgtcgc gagcggatgt gacgtggcgc acatggcgcg ccgggcatag
       61 acaggcgagc ggccgggcat atggaagctg cgcatggaaa ctcccccaat tgatggattg
      121 atttttggcg ccgagatcgc gcgcgcgtcg cgtggcagca gaggccgtgg cgatgttgcc
      181 cacgcgcagc tcagcgtgca agccccgtca cctgagccat tcgcgataag tcggatgttg
      241 gtccttcatg tcctcgtaaa gccagaccat acggtcgaca aaccacagct tgcaaatcat
      301 gacgaggaca agccccaaca gcgcgggcca gatttcgagc cacaccaacc cccaaacgaa
      361 gaaaaccgca cctacggctg agaatgcgga taagatgttc ggcaccagcc gatgatgctc
      421 aggcaccggc acctcgcggc ggttcagcca aacgcgttcg cccagcacgc ccttggaagc
      481 ccaattgttg gttgaccggg gtggcgggaa agcacgcgga ttgatccaca gccagaggat
      541 catgccagca atcggcagaa gcgaccacca gccgatccac acacggctcc acaccgccag
      601 catcaagaga ggcagcgaga tgactcgggt ccagacgctc caggggttgg cgtgccgcgc
      661 ccacgtgtcg tcatccatac ggaaaagacc cgcgaggcgg cgctcgaacg tcattggtcc
      721 tccttcagcc gagaaccagg gctcaggcga actccatgat gacagcgtcg accgcaaggc
      781 tgtcacctgg cttggcattg acggccttca caatgccgtc ccgctccgcc cgcagggtat
      841 tctccatctt catggcttcc acaacggcga gcggatcgcc agctttcacc tcttggccag
      901 cctcaacatg gatggctttc acgaggcctg gcattgggca caaaagcacc ttggacgtat
      961 cggcggcggc cttgaccggc atcagcgccg cgagctctgc ttcccgctcc gtgtaaacgc
     1021 gagccggcac gcgcatgccg cggtaggcca gctcgaaacc gttgaggatc gggcgaacct
     1081 gaaccgacac gtcgcggtcg cgcaccatgc ccgaccagac gggctcaccc ggccaccatt
     1141 tcgaggcgat ttcgagcgtg tcggacgggt ttccgtcgtc gtcgagcaac gtcaccgtga
     1201 ttggcccatc cagctcgccg tccacaatca cgcgacgacg gacaccattt ccaacatcga
     1261 cgacgcggcg cttcgcgaag gaaacagccc gacccggcat ctgacctgtg atcttccgcc
     1321 gtcggcagtt gtggaggtga tcaatggcag cggcgacggc tgccagaatc tgcagctctt
     1381 cccctccggg ggcccgggcc tcgaagccgt tgggatactc ctcctgaatg aaggtcgtcg
     1441 acagatcgcc ctcacgccag cgcgggtgct gcatcagcgc ggtcaggaac ggaatgttgt
     1501 gcttgatgcc ctcgatatag aaggcatcaa gcgcctgcgc ctgaagctcg atggccgctt
     1561 cgcgcgttgg tgcatgcgta ataagcttgg cgatcatcgg gtcgtagtac atggagatct
     1621 cgccaccctc ggccacgccg gtgtcgatgc gcacggccat gccttcggcc gagccctcga
     1681 caggagggcg gtacttcacg agacgaccgg tggacggcag gaagttgcgg aacgggtcct
     1741 cggcgtaaat gcggctctcg accgaccagc cggtgaaggt gacgtcctcc tggcgaagtc
     1801 tcagctcctc accggcggca atgcggatca tctgctccac gaggtcgatg ccggtgatca
     1861 tctccgtgac cggatgctcg acctgcaggc gcgtgttcat ctccaggaag tagaagctct
     1921 tgtcctgtcc tgcgacgaac tccaccgtgc cagcggagtc gtagccgacc gccttagcca
     1981 gagcgaccgc ctgctcgccc atggccttgc gggtcttctc gtcaagcagt ggtgatggcg
     2041 cttcctcgat gaccttctga ttgcggcgct ggatagagca ttcgcgctcg ccgagatgaa
     2101 tcacgttgcc gtgcttatcc cccagcacct gaatttctat gtgccgcgga ttgacaatga
     2161 atttctcgat gaaaacccga tcgtccccga agctcgcttt cgcctccgag cgtgcggcgt
     2221 cgaactcttc ctccagttcc ctgcgggtgt gggcgatgcg catgcctttg ccgccgccgc
     2281 cagccgacgc cttgatcatc acgggatagc cgatctcctc ggcgatgcgc gccgcctcgg
     2341 cggagtcggc gatctcgccg aggtagcctg gaaccgtcga gaccttcgcc ttggccgcgg
     2401 cgcgtttcga ctcgatcttg tcgcccatgg ccgcgatcgc cttgggattg gggccgatga
     2461 acacgatgcc cgcttccgcc agcgccagcg gaaaggcctc ccgctccgac aggaagccgt
     2521 agcccggatg caccgcctcg gccccggtct tcttgcaggc ttcgatgatg ttttcgatca
     2581 ccagatacga ctgcgcggcc ggcgccggcc cgatgtggac ggcctcatcc gccatctcga
     2641 catgcagcgc atcccggtcc gcgtccgagt aaa
//
LOCUS       DASUMG010000199         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_828902, whole genome shotgun sequence.
ACCESSION   DASUMG010000199 DASUMG010000000
VERSION     DASUMG010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828902"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2019)
                     /gene="tkt"
                     /locus_tag="VFX46_03265"
     CDS             complement(<1..2019)
                     /gene="tkt"
                     /locus_tag="VFX46_03265"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5599635.1"
                     /translation="MSTATTTASDLAQIDQLSINTIRFLSVDAVEKANSGHPGLPMGA
                     APMAYVLWTRFLKHNPTNPSWANRDRFVLSAGHGSMLLYSLLYLTGYDVPLEQIKQFR
                     EWGSTTPGHPEHGLTPGVEVTTGPLGQGFANGVGMAIAEAYLAARYNRPDHEIVNHYT
                     YALVSDGDLMEGVSAEAASLAGHLKLGKLIYLYDDNHITLSAATHITFTEERAMRFAA
                     YGWHTQTVENGNDLSAIERALRAAQAEKERPSLILVRTHIGYGSPHKQDTFEAHGSPL
                     GQEEVKLTKQALGWPIDPPFYTPEKALAHFRLAVENGKRAEAEWNARVSVYLKAFPNL
                     AREFQQGLAGELPKGWDTDIPIFAADPKGMATRVASGKVLNSIDPKLPSLIGGSADLD
                     PSTHTALTAMGDFENPSDESGNLQGSTGGGWSYVGRNLHFGVREHAMGSIANGLAVHG
                     GVIPFTATFLIFSDYMRPPIRLAALMEQRVIFVFTHDSIALGQDGPTHQPVEQLASLR
                     AIPHLIVIRPCDANETAVAWRVAIETRDRPVALVLTRQDVPTLDRAHYAAADGLRRGA
                     YVLADAPNGKPDIILIGTGSEVSLIVAAGHRLTAKEINVRIVSMPSWELFDTQPRSYR
                     DAVLPPSVRARLAVEAGASQGWCKYAGDEGDVIGVDRFGASAPGEVVMR"
     gene            complement(2031..3686)
                     /gene="pgi"
                     /locus_tag="VFX46_03270"
     CDS             complement(2031..3686)
                     /gene="pgi"
                     /locus_tag="VFX46_03270"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX5599636.1"
                     /translation="MTTNPRAQAQLRTRLPAWKALAEHYQRIRNVHLRDLFAGDPQRG
                     ERLTAEAAGLYLDYSKNRVTDETIALLLRLAHECGLAERIEAMFSGEKINVTEQRAVL
                     HIALRAPENERIFCDGVDVVPEVRAVLDRMAAFSEAIRSGGWRGYTDRPIRNVINIGI
                     GGSDLGPVMAYEALRHYSRRDMTFRFVSNVDGTDFDETTRDLDPEETLFIVCSKTFTT
                     LETLTNAHTARAWSLRKLVDERAVRRHFVAVSTNAEGVAQFGIDTANMFGFWDWVGGR
                     YSIDSAVGLSTMLAVGPENFRAMLSGFHEIDRHFRTAPFERNLPVLMGLLAVWNNDFL
                     GAQTVAVLPYDQYLKRFPAYLQQLTMESNGKHVTLDGRPVDSETGPIYWGEPGTNGQH
                     SFYQLIHQGTRLVPCDFVGFCQTLNPLGRHHDLLLANLFAQTEALAFGKTEDEVAAEG
                     TPSWLVPHRTFEGNRPTNTILAERMTPQTLGRLIALYEHSVFTQGTIWNIDSFDQWGV
                     ELGKVLANRIVPELESADEPLLAHDSSTNALIRRYRRLHRKQA"
     gene            complement(3683..>4365)
                     /locus_tag="VFX46_03275"
     CDS             complement(3683..>4365)
                     /locus_tag="VFX46_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEX5599637.1"
                     /translation="TREGVPAIEESIFAGVPINVTLLFSPEQYIASAEAYMGGIERRI
                     AAGLEPKVDSVASLFVSRWDVAVKDSLPKALRNRLGIAIAKRTYKVYRDLLASPRWRK
                     LATAGARPQRLLWASTGTKDPNASDTLYIEALAAPDTINTIPEKTLHAFADHGQVKGT
                     LPVDGGDAEEVLAEFTQIGVDHLALADQLQHDGTVSFDKSWQDLMNCIAAKSAVLKKV
                     NQAGAGQP"
BASE COUNT          879 a         1258 c         1287 g          941 t
ORIGIN      
        1 gcgcatcacg acctcccccg gtgctgaggc gccgaaacga tccactccga tcacatcacc
       61 ctcatcgccc gcatacttgc accaaccctg gctcgcgccg gcttcaaccg ccagccgtgc
      121 gcggactgat ggaggcagaa ccgcgtcgcg ataacttctg ggttgggtgt cgaacaattc
      181 ccagctgggc atggagacga tccgaacatt gatctctttc gcggtcagtc tgtgaccggc
      241 cgcaacgatc aaactgactt cggagccagt gccgattagg atgatgtccg gtttgccatt
      301 gggagcatca gctaaaacgt acgcaccgcg ccgcaagcca tctgccgcgg catagtgggc
      361 gcgatcaagt gtgggcacgt cctgccgcgt taggacgaga gccacgggcc ggtctcgtgt
      421 ctcaatcgcc acgcgccagg ccaccgccgt ctcgttcgca tcgcaggggc ggatgacaat
      481 caagtgtgga atcgcgcgga gcgaggcaag ttgctcgacc ggctgatgtg ttggaccatc
      541 ttgtccaaga gcgatgctat cgtgtgtgaa aacaaagatc actctctgct ccatcaacgc
      601 ggcgagccga atcggtggcc gcatgtaatc tgagaaaatg agaaacgtag ccgtgaacgg
      661 aatgactccg ccgtgcactg ccaacccatt cgcgatggat cccatcgcat gctcgcgcac
      721 accaaagtgt aggttgcgtc ctacatagct ccagccgccg cctgtcgatc cttgcaggtt
      781 gccggactca tcgcttgggt tctcgaaatc gcccatcgct gtaagcgccg tgtgggtgga
      841 aggatctaaa tcggcggagc cgccgattaa cgaggggagc ttcgggtcga tcgagttcaa
      901 cactttgcct gacgccacgc gggttgccat tcctttcggg tcggccgcaa agatcgggat
      961 gtcagtgtcc cagcctttgg gtaattcgcc agccagtccc tgctgaaatt ctctggcgag
     1021 gttagggaac gctttcaagt agaccgagac tcttgcgttc cattccgctt cggcccgttt
     1081 tccgttttca actgcgagac ggaaatgcgc cagtgctttc tctggagtgt agaacggcgg
     1141 atcgattggc cagcccaacg cttgtttcgt caacttcact tcttcctggc cgagcggcga
     1201 gccgtgagcc tcaaaagtgt cctgcttgtg cggcgagccg tagcctatat gcgtgcgcac
     1261 gagaatcagc gaagggcgtt ccttctccgc ttgcgcagca cgcagtgcgc gctctatcgc
     1321 tgaaagatcg tttccattct caactgtctg cgtgtgccag ccatacgctg cgaaacgcat
     1381 ggcacgttct tctgtgaatg tgatgtgggt cgcagcggaa agcgtgatgt ggttgtcgtc
     1441 gtagaggtag atcaatttac cgagcttcaa atgaccggcg agcgatgcgg cttcagccga
     1501 aacgccctcc attagatcgc cgtcgctaac gagcgcgtag gtgtaatgat taacaatctc
     1561 gtggtctggt cggttatagc gagcagcgag ataagcttcc gcgatcgcca ttccaacgcc
     1621 attggcgaat ccctggccaa gcggacctgt cgtcacctcc acccctggcg tcaatccgtg
     1681 ttcgggatga ccgggcgtag tgctgcccca ttcacgaaac tgtttgattt gttcgagagg
     1741 gacatcatag ccagtgaggt agagcagact ataaagcagc atcgatccat gcccggcgga
     1801 aagtacaaag cgatcgcgat tcgcccaact tggattggtc ggattatgtt tgaggaaccg
     1861 cgtccaaaga acataggcca ttggagctgc gcccatcggc aaaccgggat gaccgctgtt
     1921 tgctttctcc acagcgtcca ccgacaagaa acggatcgtg ttgattgaca gttgatcgat
     1981 ttgagcgaga tctgaagctg tcgtggtagc tgtactcatt attaatgttc ctatgcttgc
     2041 ttcctgtgaa gccggcggta gcgcctaatc agtgcgttgg tcgaactgtc gtgcgcgaga
     2101 aggggctcgt ccgcgctctc gagttcggga acgattcgat ttgccagcac tttgccgagc
     2161 tcaactcccc actgatcgaa cgagtcgata ttccagatcg tcccttgcgt aaacacactg
     2221 tgttcgtaga gcgcgatcag tcgccccagt gtctgcggtg tcatgcgttc cgcaaggata
     2281 gtatttgttg gtcgattacc ctcgaaggtg cgatgcggta ccagccagct tggagttccc
     2341 tccgctgcga cttcatcttc tgtcttgccg aacgccagcg cttcggtctg cgcgaacaga
     2401 tttgccagca acaaatcgtg atggcggccc agcgggttca aggtctggca gaagccgaca
     2461 aaatcacagg gaacgagccg ggtcccctga tgaatgagct gatagaacga atgctggccg
     2521 ttggtgccag gctcacccca gtagattggc cccgtctcgg agtcgacggg cctgccgtca
     2581 agcgtcacat gctttccgtt gctttccatc gtcaactgct gaagatacgc gggaaagcgc
     2641 ttcaggtatt ggtcatatgg cagcaccgcg accgtttgcg caccgaggaa atcattgttc
     2701 caaaccgcga gtagccccat gagcaccggc aggttgcgct cgaacggcgc ggtccggaaa
     2761 tgccgatcga tctcgtgaaa gcccgagagc atggcccgaa agttctccgg gccgaccgcg
     2821 agcatggtcg acaagccgac ggccgagtct atcgagtagc gaccaccgac ccagtcccag
     2881 aagccgaaca tgttcgcggt atcgatcccg aactgcgcca ctccctcggc attggtcgat
     2941 accgcgacaa agtgccggcg taccgcccgt tcgtccacta gcttgcgcag actccaggcg
     3001 cgagccgtgt gagcgttggt caacgtctca agtgtcgtga acgtcttgga gcagacgatg
     3061 aaaagtgtct cctcgggatc gaggtcacgc gtggtttcgt cgaagtcggt gccatcaacg
     3121 ttggagacga agcggaaggt catatcgcgc cggctgtaat gacgcaacgc ctcgtaggcc
     3181 attaccgggc ccagatcaga gccaccgatg ccgatgttaa tgacgttgcg gataggccga
     3241 tccgtgtatc cacgccagcc gccgctgcgg attgcttcgg agaaagccgc catgcggtcg
     3301 aggaccgcac ggacctcggg cacgacgtcg acgccgtcgc aaaaaatccg ctcgttttcc
     3361 ggcgcgcgca aggcgatatg gagaacggcg cgctgctcgg tcacgttgat tttctcaccg
     3421 ctgaacatcg cttcgatgcg ctcggccaaa ccgcattcat gagccaagcg taacagcagc
     3481 gcgatcgtct cgtcggtgac gcgattcttc gagtagtcaa gatagaggcc tgcggcttct
     3541 gcggtcagcc gctcaccgcg ctggggatcg ccggcgaaga ggtcccgcag atgcacattc
     3601 cgtattcttt ggtaatgctc ggcgagcgcc ttccacgctg gaagcctggt ccgcaattga
     3661 gcttgcgctc ttgggttcgt ggtcatggct gccccgcacc ggcttgatta accttcttca
     3721 gcacggcgct ctttgcggct atgcagttca taagatcctg ccacgacttg tcgaaggata
     3781 cggttccgtc atgctgcagt tggtcggcaa gcgctaagtg gtccacaccg atctgtgtga
     3841 attctgcgag tacttcttcg gcgtcgccgc catcaactgg caatgtgcct ttcacctgac
     3901 cgtgatcggc aaaggcgtgc aaggtctttt ctgggatggt attgatggta tcgggagccg
     3961 ccagcgcttc gatgtaaagc gtatctgagg cgttgggatc cttggtgccg gtgctggccc
     4021 agagcaggcg ttgtggccgc gcgccggcgg tggcaagttt ccgccagcgg ggtgatgcga
     4081 gcagatcgcg gtagacttta tatgtgcgct tggcgatggc gatgccaaga cgattgcgaa
     4141 gagccttcgg aaggctgtct ttgaccgcca cgtcccagcg gctgacaaaa agcgatgcga
     4201 ctgaatcaac cttcggctca aggccggcgg cgattcgtcg ttcaattccg cccatgtacg
     4261 cctcggcgga agcgatgtac tgctcgggtg aaaacaggag cgtgacattt atcggcacgc
     4321 cggcgaagat cgattcctcg atggcgggaa caccttcgcg agtac
//
LOCUS       DASUMG010000200         4400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_990434, whole genome shotgun sequence.
ACCESSION   DASUMG010000200 DASUMG010000000
VERSION     DASUMG010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4400
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990434"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFX46_03280"
     CDS             complement(<1..383)
                     /locus_tag="VFX46_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX5599638.1"
                     /translation="MRRLMMVILVALGIGAVGVYLSAFVVQQYQQALVLEFGKPKRIV
                     DQAGLHWKLPIVETVEFFDKRILDLDTTPQELFSSDQNKLIVDSFARYRIVDPLRYYQ
                     TVRNDLGVRSRVGPILDSALRRVLGS"
     gene            complement(398..1516)
                     /gene="hflK"
                     /locus_tag="VFX46_03285"
     CDS             complement(398..1516)
                     /gene="hflK"
                     /locus_tag="VFX46_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEX5599639.1"
                     /translation="MPWNNQSSGGGWKSGGGGGGPWGGGGPWGQGPSGGGQPDLEEIL
                     KRGQDKMKQVMHGSGIPGPLVLLLAAVALAVVGFYAFTFTVRPDEVGVVLRFGQLHRQ
                     EPPGLHFRLPYPIEEVRLPKVTAANRTEVGFRGPTDDRNRAIQSQEVLDESLMLTGDE
                     NIVDIGFVVLWNIKDAPAYLFNIENPEKTVKEVAESAMREVVGKSRVQDVLTEKRSQI
                     EQEVHQLVQRTLDFYGAGINVTQLQMQRAEAPAQVIDAFRDVAAAAIDQRRIENQAHA
                     YANKVIPEARGEVQRILQEAQAYKERVIAEAQGEASRFLNVLEEYRKAPELMRQRLYL
                     ETMERVLASTDKVIIDSNGGPGVVPYLPLDQLQPRKQQ"
     gene            complement(1709..2239)
                     /locus_tag="VFX46_03290"
     CDS             complement(1709..2239)
                     /locus_tag="VFX46_03290"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX5599640.1"
                     /translation="MTDKTSSEPIIALVVAAAENDVIGRSGGLPWRIPSDLRVFRRIT
                     MGKPVIMGRRTFTSIGKPLDGRDNIVLSRDPTFAAEGIVVRPTLAAAIAEARGIAATK
                     GVNEIMVIGGSEVFREALPHAQRIYLTRVHGSPEGDVYLPPIPQDQWIEASRQPLPRG
                     EADEFACTLIILERKR"
     gene            complement(2236..3030)
                     /locus_tag="VFX46_03295"
     CDS             complement(2236..3030)
                     /locus_tag="VFX46_03295"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX5599641.1"
                     /translation="MDQYLDLMRRVRTEGVRKTDRTGTGTLSIFGHQMRFDLARGFPL
                     VTTKKLHLKSIIHELIWFLKGDTNIAYLKANGVSIWDEWADEDGDLGPVYGKQWRSWA
                     TPDGRTIDQIAEAVEALRTNPDSRRIIVTAWNPADIPDMALAPCHCLFQFYVAEGRLS
                     CQLYQRSADVFLGVPFNIASYALLTMMMAQVTGLQPGEFIHTFGDAHLYLNHLEQADL
                     QLTRRPRPLPTMRLNPEVRDIFAFRYEDFELTGYDPHPHIKAPVAV"
     gene            complement(3066..3758)
                     /locus_tag="VFX46_03300"
     CDS             complement(3066..3758)
                     /locus_tag="VFX46_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX5599642.1"
                     /translation="MAAAFTIIAISGLLATALVGTLEIAPPTPFPAPDAIPRDEARIW
                     RMAAWLLGMQAAIIVLVVWVSGLFGGNRAEVLALNRSTPGRLYVAAILVMLAAQVIYN
                     VIVLPFAHQTMVEDVQPLIEPLQSDAFWLFGLAIVVGAPLSEELLFRGFLLSALAQSR
                     LGYFGAALLTTLGWTVLHAGYSGLGLIEVFLAGLLFSWLLWRTGNLWVPVACHAFYNG
                     IVLFAIVAVLLA"
     gene            3862..4224
                     /gene="ssrA"
                     /locus_tag="VFX46_03305"
     tmRNA           3862..4224
                     /gene="ssrA"
                     /locus_tag="VFX46_03305"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
BASE COUNT          832 a         1356 c         1377 g          835 t
ORIGIN      
        1 gagcctagga cgcgccgcag ggcactgtcg agaatcgggc cgacacggga gcggacgcca
       61 agatcgttgc gcaccgtctg gtaatagcgc agcgggtcga cgatccggta tctggcgaag
      121 ctgtcgacga tgagcttgtt ctggtccgaa gagaacagct cctgcggtgt cgtatccagg
      181 tcgaggatgc gcttgtcgaa gaactcaacc gtctcaacga tcggcaactt ccagtgcagc
      241 cccgcctggt cgacgatccg ttttggcttg ccgaactcaa ggaccaaggc ctgctgatac
      301 tgctgcacga cgaaagccga gagatagact cccacggccc cgatcccgag agcgacgagg
      361 attaccatca taagtctgcg catgattgga aatcctctta ctgctgctta cggggttgta
      421 attggtccag tggaaggtac ggcaccacgc ctggcccccc attgctgtcg atgatgacct
      481 tgtcggtcga ggcgaggacg cgctccatcg tttccagata gagccgctgc cgcatcagct
      541 ctggcgcctt gcggtattcc tcaaggacgt tcaggaaccg gcttgcctca ccctgggcct
      601 cagcgatgac gcgctcctta tacgcctgcg cttcctgaag gatgcgctgc acctcaccac
      661 gagcctctgg aataactttg ttggcatacg cgtgggcctg gttttcgatt ctccgctggt
      721 cgatcgctgc cgccgcgacg tcacggaacg catcgatgac ctgcgcaggt gcttcggccc
      781 gctgcatctg gagctgcgtg acgttgatcc ccgcgccgta gaagtcgagc gtgcgctgca
      841 cgagctggtg cacttcctgc tcgatctgcg agcgcttttc agtgagcacg tcctgaaccc
      901 ggctcttgcc caccacctcg cgcatggcgc tctctgccac ctctttgacg gtcttctcgg
      961 ggttttcgat attgaagagg taggccggag cgtcctttat gttccacagc acgacgaagc
     1021 cgatgtcgac gatattctcg tcaccggtga gcataaggct ctcgtcgaga acctcttgcg
     1081 actggattgc ccggtttcga tcatcggtgg ggccgcggaa gccaacctcg gtacggttcg
     1141 cagctgtgac cttgggcagg cgcacttcct cgatgggata aggtaagcgg aagtgcagcc
     1201 ccggcggctc ctgccggtgg agctggccga aacgcaggac tacgccaact tcgtccggcc
     1261 ttacagtaaa ggtgaaggca tagaacccga cgacagcgag agccaccgca gccagcagca
     1321 ggacgagcgg gccggggatg cccgagccgt gcatcacctg cttcatcttg tcttggccac
     1381 gcttgaggat ctcctcgaga tcgggctgac caccgccact ggggccctgc ccccatggtc
     1441 cgccgcctcc ccatggtcca ccaccgccgc cgccgctctt ccagccgccg ccgctactct
     1501 gattgttcca aggcatcgaa cagtgcttcc gtttcctgct ttgtgctggg agtgccaaac
     1561 ggcgcccgcc gcgagccgcg gggcgccgag acgtgcgttt ttgtatacgt ggttataggg
     1621 gacacccttc gcgcgcgcaa accgatctct gcaaaatgtg gtgcggccga tggggcttca
     1681 agcatttacg tttggcttcg tagccaaact agcgcttgcg ttcgagaatg atcaacgtgc
     1741 aagcgaattc gtcagcctct cctcgcggca gcggctgacg cgaggcctcg atccactgat
     1801 cttgggggat cggcggcaga tagacatccc cctctggcga gccatggacg cgagtgaggt
     1861 agatccgttg cgcgtgaggc aacgcttcgc ggaacacctc cgagccgccg ataaccatga
     1921 tttcgtttac gcccttggtt gccgcgatgc cccgcgcttc agcaattgcg gcggcaaggg
     1981 ttggcctgac aacgatgccc tcggcggcaa aggtcgggtc gcggctcaac acaatattgt
     2041 cgcgtccgtc gagcggcttg ccgatcgagg tgaacgtccg ccggcccatg atcaccggtt
     2101 tgcccatcgt aatccgccgg aagacccgca agtccgacgg gatccgccaa ggaagccctc
     2161 cgctgcggcc gatcacgtcg ttctcggcag cggcgaccac caaagcaatg atgggctcgg
     2221 aacttgtctt gtccgtcata ccgccaccgg cgccttgatg tgcggatgcg gatcatagcc
     2281 ggtgagctcg aaatcctcgt accggaacgc aaaaatgtcc ctcacctcgg ggttgagtct
     2341 catcgtcggc agtggtcgcg gcctcctggt gagctgcagg tcggcttgct cgaggtggtt
     2401 caagtagagg tgcgcgtcgc cgaaggtgtg gatgaactcg cccggctgca ggcccgtgac
     2461 ctgtgccatc atcatcgtta gcagcgcgta gctggcgatg ttgaagggaa cgcccaggaa
     2521 cacatcggcg gaacgctggt agagttggca ggagagccgg ccctcggcga cgtaaaactg
     2581 gaacaggcag tggcagggtg ctagcgccat gtctggaatg tcggccgggt tccaggctgt
     2641 cacgatgatg cggcggctgt ccgggtttgt cctcaacgct tcaactgctt cggcgatctg
     2701 gtcgatcgtg cggccatctg gcgtggccca agagcgccat tgtttcccat agacgggccc
     2761 gagatcgccg tcctcgtccg cccattcgtc ccaaattgag accccgttcg ccttcaggta
     2821 ggcgatgttc gtgtccccct tcaggaacca gatcagctcg tgaatgatcg acttcaggtg
     2881 cagcttcttc gtggtcacca gcggaaagcc gcgggcgaga tcgaagcgca tctggtggcc
     2941 gaagatgctg agggtgcccg tgcccgtgcg gtcggtcttc ctgacgccct cggtgcgcac
     3001 gcggcgcatc agatcgagat actggtccat ggctcgttct tagcctgatt ccatctcttg
     3061 gcgcctcacg ccagtagaac ggccacgatc gcgaacagga cgatgccatt atagaaggcg
     3121 tggcaggcca ccggcaccca caagttgccc gtgcgccaca gcaaccagga gaacagaagt
     3181 ccagcgagga agacctcgat aagccccagg ccggagtagc cggcgtggag caccgtccag
     3241 ccaagtgtcg taagcagcgc ggcgccgaaa tagccgagcc gcgattgtgc aagtgcgctc
     3301 agcaggaagc cccggaaaag cagctcctcg gacagcggtg ctccgacgac gatggcaagg
     3361 ccgaacagcc agaaagcgtc ggactgcagc ggctcgatga gcggctgcac gtcctccacc
     3421 atcgtctggt gggcaaaggg gagcacgatg acattgtaga tcacctgggc ggccagcatc
     3481 acgaggatgg cggcaacgta caggcggccc ggcgtggacc ggttgagggc taacacctcc
     3541 gctctgtttc cgccgaacag gcccgaaacc cacacgacca gcacgatgat cgccgcctgc
     3601 atgccaagta gccatgcggc cattctccag atccgggctt cgtccctcgg gatcgcgtcg
     3661 ggtgcgggaa agggagtcgg cggcgcgatc tccagtgtcc ccacgagggc cgtggcaagg
     3721 aggccagaga tcgcgatgat ggtgaaagct gccgccacgg cgggcaccgg gcgccaggga
     3781 gagcgcgcat cataggctgg cgggcctgtc agcagctcgc gtatggcaga ggtgagcaat
     3841 agcggtcata ccctttcaag agttcttttt ttcgagccat gggcagccta tattaggcgt
     3901 gccggattcg tccggctatg gcgataaatg gccgtcgaaa taagcccatc ggacccgggg
     3961 gcagtacccg gcgcctccac cacaggccgc agcaagagcg gcggtctctg atgggggcga
     4021 aataggatcg acgagggtgt aaaggacgtg ctttcgcccg gcatggttcc gccgttatcg
     4081 ggccaaccag aatagttgcc aacgacaact atgctgaggc tgctctcgct gcgtaatgca
     4141 gcgcgaaatg cctgaaccca agtcctgatc actagcctgg tcaggcgcgg ttcggagggc
     4201 accgggcaac agaagccctc cacttccctt gcggcccatg cctgggttcg cgcagcagta
     4261 gatggccctg gtccctcatg agcagcgggc gcgtgatcga ctatgaagct ttggcgcagg
     4321 acgcgatgcg aggcgtcgtg cgcgccgtgc tagcccgtac cgcaaagagc gggcttccgg
     4381 gtgatcacca cttctatatt
//
LOCUS       DASUMG010000201         3015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_347904, whole genome shotgun sequence.
ACCESSION   DASUMG010000201 DASUMG010000000
VERSION     DASUMG010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347904"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1237)
                     /locus_tag="VFX46_03310"
     CDS             complement(80..1237)
                     /locus_tag="VFX46_03310"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX5599643.1"
                     /translation="MRRADLIALAQSTGFPRVSIYLPTHLTYPEIEQDPIRLSNLLKE
                     AEERLQAAGVRRPEIEELLAEAKGRVPASMFWRYQEHGLAVLIEKGKTHWVKLPRSVP
                     ELVVVSPRYHLRPMIPMFRNGENFNLLAVSREKIRFYRGRERELIEVEVEGMPRGMAE
                     IRQRTEYDDQLSFHARDRGDPASGGNHGKFHSLGVSPEDYENVLFDTLLREVAKAVDR
                     HLASSEEPLVLAAEPRTLGHLRKHLTYRHIVQDAIQSDPVGLNKEELHSEAWRIAQPI
                     IRADRENARRRLRAWLEGAAIPGTKDPQMLLRDAIAGRVECIFVAQNANVWGFFDEEF
                     QVLRIDPEPGPGNEDILNRLAIETLIRSGDVFTMPEDFEARLGPVAGLYRY"
     gene            1620..2252
                     /locus_tag="VFX46_03315"
     CDS             1620..2252
                     /locus_tag="VFX46_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5599644.1"
                     /translation="MRHWAAALAGLLVAIPLAAADPLDGHDCFSSDNDRRIKGCTELI
                     ERPEVTGQLLGSAYAMRALGYSLKGLYDEAIRDYDEAIRLVPDFAVALNNRAWAYFKS
                     GRAEKGLPDVERSLELQPTSPHAYDTRAHIRQVMGDQEGALRDYEAAMRFGGERMVQL
                     YQCGLAALGLYGGPVDGIYTPILRRAIEACVTSKTCDPLPPDEECRAVTS"
     gene            2326..2853
                     /locus_tag="VFX46_03320"
     CDS             2326..2853
                     /locus_tag="VFX46_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEX5599645.1"
                     /translation="MKFLASFAAALVLSLLGMTASAQESSSVSANGITISQPWARATP
                     GGVKNGAAYLQISASGTADKLVDARSDAAERVELHNHVQENGVMRMRRVESIEVPAGG
                     TVTLAPGGYHLMLMGLKQPLKAGEKLDLTLVFEKAGEINVTAPIEPIGAKGPGHDHGH
                     DHGHGHGERQHEHKH"
BASE COUNT          596 a          943 c          894 g          582 t
ORIGIN      
        1 aaatctcatc ttcaaaatgc atcggccacc gcctcgagcg gtggccgata gtttttttgg
       61 tttaatatca gtcgactgcc taatacctat acagccctgc caccggcccg agcctggctt
      121 caaaatcctc cggcatcgta aagacgtcgc cgctgcggat cagggtctcg atggccaagc
      181 gattaagaat gtcctcgttg ccggggcctg gctcaggatc gatgcgaagc acctgaaact
      241 cctcatcgaa gaagccccaa acgttcgcat tttgggccac gaagatgcat tcgacccgcc
      301 cggctatcgc atcccgaagc agcatctgtg gatccttcgt gccgggaatg gcagcccctt
      361 cgagccaagc ccgcaggcgc cgccgcgcat tctcgcgatc ggcgcggatg atcggctgag
      421 caatgcgcca cgcttcggaa tgaagctctt ccttattcaa cccaacgggg tccgattgga
      481 tcgcatcctg cacgatgtgc cgataagtca gatgcttgcg aagatgcccc aatgtgcgcg
      541 gctcggctgc cagaacgagc ggctcttcgc tgctcgccaa gtggcggtcg acagctttgg
      601 ccacctcccg aagcaacgtg tcgaacaaga cattttcata atcttcgggg ctcacgccga
      661 gcgaatggaa cttgccgtga ttgcctccgc tcgccggatc gccgcggtcg cgggcatgaa
      721 agctgagctg atcgtcatat tccgtgcgct gccggatttc cgccatgccg cgcggcatgc
      781 cctcgacttc gacctcaatg agctcacgct cgcgtccccg atagaaccgg atcttctcgc
      841 gcgagactgc gagcaaattg aaattctcgc cgttccggaa catcggaatc atcggacgca
      901 gatgataccg tggactgacg acaacgagct ccggaacaga ccggggcaat ttcacccaat
      961 gcgtttttcc cttctcgata agcacggcga ggccgtgctc ctgatagcgc cagaacatgg
     1021 atgcgggcac acggcccttc gcttcggcaa gaagctcctc gatctccggc cggcggacgc
     1081 ccgctgcttg cagtcgctct tcggcctcct taagcaggtt cgatagtcgt attgggtctt
     1141 gctcgatttc cgggtaggta aggtgtgtcg ggagatagat gctaactctt ggaaaaccgg
     1201 tcgactgtgc tagcgcgatg agatcggctc ttctcataag ctttcccact tttgtcagct
     1261 ggccttgagg agcgtaagca aagacgccag cttttctgtt cgtgtgctcc ggcccgttaa
     1321 ggaaaactcc actccggtct gccagttccg cgaccggagc cacggtatcc ccacaggcct
     1381 tgactgcagc ctacccacag cgctgctgac ccagccttga catttgccaa tatcgagcag
     1441 gaattcgggt atatgcaatg gccgagcggt gcccataagt taagggtggg tagatcacgg
     1501 ttgggtacaa tcccgcttgc gggtgacaag cttcatttca gcaacgccat cgaatcgtcg
     1561 ggttccagtc ggaaatcgtc aggaaatgga cggttaagct ccatgaaggg atgcagatca
     1621 tgagacattg ggcagcggca ctcgccggtc tcctcgtggc aatccccctg gcggcagcgg
     1681 accccttgga tggccacgac tgtttctcct ccgacaatga tcgtcgcatc aaggggtgca
     1741 cggagctgat cgagcggccc gaggtcaccg ggcagctgct ggggtccgcc tacgccatgc
     1801 gggcgctcgg ctactcgctc aaaggcctct acgacgaagc catccgcgac tacgacgagg
     1861 ccatccggct cgtgccggac ttcgccgtgg ctctcaacaa ccgggcctgg gcctatttca
     1921 agtcggggcg tgcagagaaa ggcctgcccg acgtcgagcg ctcgctggag ctgcagccga
     1981 caagcccgca cgcttatgac acgcgggcgc acatccggca ggtgatgggc gaccaagaag
     2041 gcgcgctgcg cgactacgag gccgccatgc gattcggcgg cgagcgcatg gtccagctct
     2101 accaatgcgg gcttgcggca ttgggtctct atggcggccc ggtcgacggc atctacactc
     2161 cgattctgcg gcgcgccatc gaggcatgcg tgaccagcaa gacctgcgat cccctccccc
     2221 cagacgagga atgccgcgcc gtcacgtcgt gacggatctg gtcgagcccg cctgagccct
     2281 cgacccgatc cgaaacgttt cggcatcgag tcgaggagac cagcgatgaa gtttcttgcc
     2341 agctttgctg cggctttggt cctgtccctg ctggggatga ccgcctctgc ccaggaaagc
     2401 tccagcgtga gcgcgaacgg aatcaccatt tcgcagccct gggcgcgagc aacacccggc
     2461 ggcgtgaaga acggcgcagc ttacctgcag atctcagctt cgggcaccgc tgacaagctg
     2521 gtcgacgccc ggtcggacgc agccgaacgg gtcgagttgc ataatcacgt ccaagagaac
     2581 ggcgtgatga ggatgcgccg cgtcgaatcg atcgaggtgc cagccggcgg aacggtgaca
     2641 cttgccccgg gcggctacca cctcatgctg atgggcctga agcagccgct caaggcaggt
     2701 gagaagttgg acctgacgct ggtcttcgaa aaggcgggcg agatcaacgt gacggcccca
     2761 atcgagccga tcggggccaa gggaccagga catgaccatg gtcacgatca cggccatggt
     2821 cacggtgagc gccagcacga gcacaagcac taacgcgctg ggttctttgc tctttaggga
     2881 agcggcggcg cccagctcta ggtcgccgct ttcgctttct gcttggtctt ccgccctcgc
     2941 gaagccgcct cagccttcgc ttcgtcaacg actttcagtg cataggcagg caaagcaaac
     3001 gccgcgacgt ggagc
//
LOCUS       DASUMG010000202         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1150786, whole genome shotgun sequence.
ACCESSION   DASUMG010000202 DASUMG010000000
VERSION     DASUMG010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150786"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1708)
                     /locus_tag="VFX46_03325"
     CDS             complement(281..1708)
                     /locus_tag="VFX46_03325"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX5599646.1"
                     /translation="MELSRFERLSDTVWQVPPTGKMRVPAIIYATEDLIRDMDDKVYT
                     QVTNVATLPGIVRASYAMPDAHWGYGFPIGGVAAFDPDEGGVVSAGGVGFDISCGVRT
                     HLTGLTRAEIMKVQGELADALYATIPVGVGSTGKITLDDDEMTAMLAGGARWAVERGY
                     GRPEDLERIEENGRQDDAKPSAVSAQARKRQRREMGTLGSGNHYLEVQEIVEIFDADT
                     AAAFGLKQGECVVTIHCGSRGLGHQIGTEYLREMAVAAETYGISLPDRELACAPIKSE
                     VGQRYLGAMRSGINCALANRQIIGHLARQVFKRMFGVDLPLMFDVSHNTCKVEPHAVG
                     SVARQLYVHRKGATRAFGPGHPSLPEALRPWGQPVLIGGSMGTGSYILVGTRESEQLA
                     FSSACHGAGRQMSRHEAVRTWSGRQVIDELKARGIEVRSPSLRGVAEEAPGAYKDVSA
                     VVDAAEEAGLSRKVARVAPLIVIKG"
     gene            complement(1731..2180)
                     /locus_tag="VFX46_03330"
     CDS             complement(1731..2180)
                     /locus_tag="VFX46_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX5599647.1"
                     /translation="MTSTSEKSTVNSAHWEHFQHDADIGVRGYGPSLASAFEQAALAL
                     SAVLVDPNAIERSETVEIEVEAPNSDLLLYDWLNAIIYEMGTRDMIFGAYDVSIEGNK
                     LVGRAHGEAVDRERHQPAVEIKGATMTELATGEIRPGEWRAQCVVDV"
     gene            2318..>2494
                     /locus_tag="VFX46_03335"
     CDS             2318..>2494
                     /locus_tag="VFX46_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX5599648.1"
                     /translation="MEQSKPQPHFDAPGRGRIYDSIIETIGNTPLVRAPRLSKEEGVV
                     ADLVLKLEFFNPLGS"
BASE COUNT          444 a          820 c          774 g          456 t
ORIGIN      
        1 ccagttattc tcgctcgcat acgaagcagc tctgcgcaag atgttgctgc ataaggcttc
       61 ttcaggatgg cgttgggagg aagttgccgt gttttccgag cccgctccgt tgggacgacg
      121 cctggcaggc aacgttcaat ccggatgatc cggaagcatc gcgatttgcc gacgatgatg
      181 ccgagcagcg ctggcgcgac gagggcttgg caatcgccac cgaactagag cgggagtggc
      241 cggggccagt cgtcgtgagg ctctgagctt gtcctgtgca tcaccccttg atgacgatca
      301 gtggcgccac ccgcgccacc ttgcgggaca atcctgcttc ttcggcggca tcgacgacgg
      361 cgctgacatc cttgtaagcg ccgggcgcct cctccgccac accgcgcagt gaggggctgc
      421 ggacctcgat gcccctggcc ttcagctcgt cgatcacctg ccgcccgctc cacgtgcgca
      481 cggcctcgtg ccggctcatc tggcgacccg cgccgtggca ggccgaggag aaagcaagct
      541 gctcgctttc gcgcgtgccg acgagaatgt acgaccccgt acccatgctg ccgccgatca
      601 gcaccggctg cccccatgga cgcaacgctt caggtaggct cggatgaccg gggccgaatg
      661 cgcgcgtcgc gcccttgcga tgcacgtaaa gctggcgggc aacggatccc accgcatgcg
      721 gctccacttt gcaggtgttg tgggagacgt cgaacatcag cggtaggtcc acgccgaaca
      781 tgcgcttgaa gacctggcgc gccagatggc cgatgatctg ccggttggcg agagcgcagt
      841 ttattcctga ccgcatggcg ccgagatagc gctggcccac ttccgacttg atcggcgcgc
      901 aggcgagctc ccggtcaggc agcgatatgc cgtaggtctc ggcagcaacg gccatttccc
      961 gcaaatactc ggttccgatc tggtggccga gcccgcgcga gccacagtgg atggtgacga
     1021 cgcactcgcc ctgcttcagc ccgaaggctg cagcggtgtc ggcgtcgaaa atttcgacga
     1081 tctcttgcac ctcgaggtaa tggttgcccg atcctagcgt gcccatctcg cggcgctggc
     1141 gcttgcgcgc ctgggcggag acggctgacg gcttcgcgtc gtcttgcctg ccgttctcct
     1201 cgatgcgctc cagatcctcc ggccggccgt agccgcgctc gaccgcccat cgagcgccac
     1261 cggccagcat ggccgtcatc tcgtcatcgt cgagggtgat tttgcccgtc gagccgacac
     1321 cgacgggaat ggtcgcgtaa agcgcatcgg ccagctcgcc ctgaaccttc atgatttcgg
     1381 cgcgcgtaag ccctgtgaga tgggtacgca cgccacagga gatatcgaag ccgaccccgc
     1441 cggcggatac aacgccgccc tcgtcgggat cgaacgcagc cacgccgccg atcggaaatc
     1501 cgtagcccca atgcgcatct ggcatcgcgt aggacgcgcg aacaatgccg ggcagcgtcg
     1561 cgacgttggt gacctgcgtg tagaccttgt cgtccatgtc gcggattagg tcctccgtcg
     1621 catagatgat cgcgggcacg cgcatcttgc ccgtcggagg aacctgccag accgtatccg
     1681 acagacgctc gaaccgagaa agttccatga gtgggctcct ttcgctggag ctagacgtcc
     1741 acgacgcact gggcccgcca ctcgccaggg cggatttcac cggtcgccag ctcggtcatg
     1801 gtcgcgccct tgatctcgac ggcgggctgg tggcgttcac gatcgacagc ctcaccatgg
     1861 gcgcggccaa cgagcttgtt gccttcaatg gaaacgtcgt acgcgccgaa aatcatgtcg
     1921 cgcgttccca tttcatagat gatggcgttg agccaatcgt agaggaggag atcggagttc
     1981 ggcgcctcga cctcgatttc gaccgtctcg cttcgttcga tcgcattggg atcgacaagc
     2041 acggccgaga gggcaagcgc cgcctgctcg aaggccgagg caagcgaggg cccgtatccg
     2101 cgcacaccta tatcagcgtc atgctgaaag tgctcccagt gcgctgaatt gacagtggat
     2161 ttttcactcg tgctggtcat cggcagctcc ggtcggcgct cggaacggag tccatcctcc
     2221 ctcagttatc aacgcaaaac cgtcgcgagg aggtaggcgg ttcacaattc ccgtgccgta
     2281 aagccgcaac ctccgtctcc acgcatagat cgggcccatg gaacagtcca aaccgcaacc
     2341 gcacttcgac gcgcccggac gcgggcgcat ctacgacagc atcatcgaga cgatcggcaa
     2401 cacgccgctt gtgcgtgcgc cgcggctcag caaggaggag ggcgtcgtcg ccgacctcgt
     2461 gctgaagctc gagttcttca atccgctggg cagc
//
LOCUS       DASUMG010000203         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_990563, whole genome shotgun sequence.
ACCESSION   DASUMG010000203 DASUMG010000000
VERSION     DASUMG010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990563"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VFX46_03340"
     CDS             complement(<1..933)
                     /locus_tag="VFX46_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5599649.1"
                     /translation="MRIRAVRVRELGCFAEPVALEGLSGGLDVLAGPNEFGKSTLLRA
                     LQALFTLRHTAQSRAVDRLKPYGGGAPLIEADFEAGGRLWRLRKQFVSERQALLTDLT
                     TNKVVARGEEAHQRALELIGGGSGEHCLGLLWLEQGSVLPSREGRSGENGLDGGKREV
                     LSQIVERELKAATSGGHELRIVRDKLIEERNALVTERRRQPRGEYAAAITSRNSAMQE
                     LEAARMQAQAAAKRLTRLAELRSRRAELSDPQVVANRLQRVEKGEAEVKAASASRQQL
                     KIAEAKVQACESRLAEVQQAHQLLTQGLEEVSSLE"
     gene            complement(930..>2060)
                     /locus_tag="VFX46_03345"
     CDS             complement(930..>2060)
                     /locus_tag="VFX46_03345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX5599650.1"
                     /translation="RFTFVHTADWQIGKPFAGFPADKIPLLREARLDAIGRIASLARS
                     AGAQHVLVAGDVYDSPDLPDRDLRQSIERLRREEDLVWHLLPGNHDPAQPGGVWERLI
                     RLGVPENVRLHLKPRVEMIADGVALLPAPLTMRSATADPTAWMDTAATPGAQFRIGLA
                     HGSIQGFGGEDGEAAVPIAPDRARLAGLDYLALGDWHGVTRVSDRIWYSGTPEPDRFP
                     DNEPGFALLVRVGQNGRVEVERHPTARYTWWKRSLTVTGSDALASFERDIAANVLQPE
                     RLLLKLTLRGTVSLSTWSDVTTWLETLEQRLFHLSLDNQALQVVPEAVELDEFGTGDL
                     RRVAELLSAAARDEASGRAEVASLALRKLYGLWREAREGARP"
BASE COUNT          370 a          734 c          583 g          373 t
ORIGIN      
        1 ttcgaggctg ctgacttcct cgagcccttg cgtgagcagc tgatgggcct gctgcacttc
       61 ggccaaccgg ctctcgcagg cctgcacttt cgcctcggcg atcttcagct gctggcgcga
      121 agccgatgct gccttcactt cagcttcgcc cttctctacg cgctgaagcc gattagcaac
      181 cacctgcgga tcgctcagct cggcgcgccg cgaccgcagc tcggccaaac gggtcaggcg
      241 tttcgccgcc gcctgcgcct gcatcctggc agcttccagc tcttgcatcg cggaattgcg
      301 gctcgtgatg gccgctgcgt actcgcccct gggctgccgg cgacgctcag ttaccaaggc
      361 attgcgttcc tcaatgagct tgtcgcgaac gatacgaagc tcgtgcccac cggatgtcgc
      421 agccttcaac tcacgctcga cgatctggct gagcacctcg cgcttgccgc catcgagacc
      481 gttctctcca cttcttcctt cccgcgaggg cagaacgctg ccttgctcca gccacagcag
      541 ccccaggcag tgctccccgc tgccaccgcc gatcaactcg agagcgcgct gatgagcttc
      601 ctctcctctc gcgacaacct tattggtcgt caggtccgtc aacagagcct gtctttcgct
      661 tacgaactgc tttctcagac gccaaagccg cccgccagcc tcgaagtccg cctcgattag
      721 cggcgcgcct ccgccatagg gcttcaaccg atcaaccgcc cgcgactgcg ccgtgtggcg
      781 caaagtgaac aaggcctgca aggccctgag cagggtcgac ttgccgaact cgttcggtcc
      841 ggccagcacg tcgaggccgc ccgacagccc ttccaaagca accggctcgg cgaagcagcc
      901 cagctcccgc actcggaccg cgcggatcct catggtctcg ccccctcgcg cgcctctcgc
      961 caaaggccat acagctttcg gagggcaagc gatgcgacct cggccctgcc actcgcctca
     1021 tcgcgggctg ccgcgctcag aagctcagcc acccggcgaa ggtcgcccgt tccgaactcg
     1081 tccagctcca cagcctccgg aacgacctgc aaagcctggt tgtcgagtga caaatggaac
     1141 agccgctgtt cgagggtttc cagccacgtc gtcacgtccg accaggtcga gagcgagaca
     1201 gttcctctga gcgtgagctt gagaagcaat cgctccggct gcagtacatt tgcagcgatg
     1261 tctcgctcga acgacgccag cgcgtcggag ccagtcacgg tgaggctacg cttccaccac
     1321 gtgtagcgtg cggtcggatg gcgctcgacc tccaccctcc cgttctgtcc aacccggact
     1381 aagagagcaa aaccaggctc attatccgga aatcgatccg gctccggcgt tcccgaatac
     1441 cagatgcgat cggagactcg tgtcacacca tgccagtctc cgagcgcgag gtagtcgaga
     1501 cctgctaggc gcgcacgatc tggtgcgatc ggcaccgccg cctcgccatc ctcaccgccg
     1561 aacccctgta tggacccgtg cgcgagcccg atcctgaact gtgcccctgg ggtcgcggcc
     1621 gtatccatcc aggccgttgg gtcggctgtt gcggaacgca tggtcagggg cgcgggcagc
     1681 aaagctactc cgtcggcaat catctcgacc cgcggtttga gatgcagacg cacattctct
     1741 gggacaccca gacggataag ccgctcccaa acgccaccgg gttgcgcggg gtcatgattc
     1801 ccgggaagca aatgccagac gagatcctct tcgcgcctca gccgttcaat cgattgccgg
     1861 aggtcccgat ccggcaagtc gggcgaatca tagacgtcgc ctgcgacgag gacatgctgc
     1921 gcgcctgcgc tccgcgccaa gctggcgatc cgaccaatgg catcgagccg cgcctccctt
     1981 agcagaggaa tcttgtcggc aggaaagcca gcgaatggct tcccgatctg ccaatcggcc
     2041 gtgtgaacaa atgtgaaacg
//
LOCUS       DASUMG010000204         5121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_669601, whole genome shotgun sequence.
ACCESSION   DASUMG010000204 DASUMG010000000
VERSION     DASUMG010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669601"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFX46_03350"
     CDS             complement(<1..823)
                     /locus_tag="VFX46_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5599651.1"
                     /translation="MFRWFETRINAFPEEMPERPPDKLWAFYWHFLRPVWPAFLLLLI
                     VGLVGSLIEVALYAFIGTIVDRMKAAQSPQSFISDNGSMLVFMAFVALILRPLVSTAH
                     DLTKNQMIAGPVQTRVRWLSHRYVLRQSLGFFQNDFAGRVASKVLQTGHALRESVVQV
                     IDGLWFGVVHWGAALVLFADADWRLMIPLLAWLAAYGATIRVFVPRIQKLSFNASEAR
                     SVLTGRVVDSYTNILTVKLFAHADREDESARWALQNHLDRFQESQRNLTQMEFVLY"
     gene            1010..1918
                     /gene="hemF"
                     /locus_tag="VFX46_03355"
     CDS             1010..1918
                     /gene="hemF"
                     /locus_tag="VFX46_03355"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEX5599652.1"
                     /translation="MLPQPDVLDQWRERAAAWFETLRDEIRTVFEQLEDELPPGAPLS
                     DRPPGRFVATPWNRTDHTGAPGGGGVMSMMRGRVFEKVGVHISTVFGEFAPEFRSQIP
                     GASNDPRFWASGISLIAHTQNPHVPAAHMNTRMVVTTRWWFGGGADLTPMLEARRTQS
                     DPDTIAFHEAMRAACAPHSVADYARFKAWCDEYFYLPHRKEMRGVGGIFYDYLTPTEE
                     EGGWEAAFAFTQDVGRAFRDIYPQLVRRNWEKPWTEAEREEQLVRRGRYVEFNLLYDR
                     GTIFGLKTGGNVDSILSSMPPVVKWP"
     gene            complement(1949..3331)
                     /locus_tag="VFX46_03360"
     CDS             complement(1949..3331)
                     /locus_tag="VFX46_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5599653.1"
                     /translation="MRLNSLAFRLFATAVVSTMLILPVAGLLIYSVYWQEVEEGFNER
                     LSQLLTIVHADAIDGAESQPGTPGQVGEPLFFITHSGWYWQIKPLGGAPGEIKVSPSL
                     ATFILPSPYEAGVEPDLQGTRWMDGLGPNDQRVRIAETIYAFGEGADAPQYSISVAGP
                     LDWLDARVAGFGTQLAMALALAGVALVAVTLIQVRFGLLPLRAIENGLAAIRSGKATR
                     LDGELPAEIEPLQNELNALIQSNEEIIERARTQVGNLAHALKTPLAVITNEARDDKSP
                     LAQKVAEQAKVMRDQINHYLDRARKVATAGSLVRVTEVLPVVEALQRALERIYRDKGI
                     AILVDCPESARFQGEQHDLEEMLGNLLDNACKWARKNVRFSAVIKPPSQRGGGSRLIV
                     TIEDDGPGLTTDQRRRIGKRGLRLDETKPGSGLGLSIVSDLAHLYRGRLDLADSRYGG
                     LKAILELPAP"
     gene            complement(3321..3992)
                     /locus_tag="VFX46_03365"
     CDS             complement(3321..3992)
                     /locus_tag="VFX46_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599654.1"
                     /translation="MRALVVEDDPDLNRQLRQALSDAGFAVDTAHDGEEGFFLGDTEP
                     YDIVILDIGLPKMDGISILEQWRRAGRNMPVIILTARDRWSDKVAGIDAGADDYLAKP
                     FHMEELLARVRAQVRRFAGHAKSEIECGPLRLDTRTARVTCNGQPIKLTSHEYRLLAY
                     LMHHNERVVSRTELVEHLYDQDFDRDSNTIEVFIGRLRKKIPADMIKTVRGLGYRLTQ
                     EPDEA"
     gene            complement(4059..4358)
                     /locus_tag="VFX46_03370"
     CDS             complement(4059..4358)
                     /locus_tag="VFX46_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599655.1"
                     /translation="MLRIATTLIALIVLADLATATAETNQCFKSWSEAAAVVRQERLV
                     AVEQLSKQARATLGGSIVKATLCSAGQRYFYRLVVRLPGGLMRHISLDARQPSER"
     gene            complement(4423..4713)
                     /locus_tag="VFX46_03375"
     CDS             complement(4423..4713)
                     /locus_tag="VFX46_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599656.1"
                     /translation="MSQTEVSQTDQTSQTSKWWAHSMTIWGAIITAASTILPTIGPLF
                     GIDITPDLVHELGDQVVLVVQAIGGLIGIIMTIYGRTRATATLERKQVTFQL"
     gene            4768..5061
                     /locus_tag="VFX46_03380"
     CDS             4768..5061
                     /locus_tag="VFX46_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599657.1"
                     /translation="MAGRFNCAIDPPEPSPPETPEVTKAAVARTSDVRVALEDLGYGL
                     SMRGGERLGSDYSVDIHPQGLLQHSCRSADTMVHRGIEVHDGKRRREVACPGW"
BASE COUNT         1011 a         1557 c         1576 g          977 t
ORIGIN      
        1 aatagaggac gaactccatt tgggtgaggt tgcgctggct ttcttgaaac cggtccaggt
       61 ggttctgcag cgcccaacgc gcactctcat cttcacgatc cgcgtgcgca aagagcttca
      121 cggtaaggat gttggtatag ctgtcgacga ctcgcccagt cagcaccgag cgtgcttcag
      181 aggcattgaa cgacagcttc tgaatgcggg gaacgaatac ccggattgta gcaccatatg
      241 ctgccagcca ggccaggagg gggatcatca ggcgccaatc cgcatcggcg aaaagtacga
      301 gcgctgctcc ccaatggacg acgccaaacc acaggccatc tatgacttgc acgactgact
      361 cgcgaagcgc gtggcccgtc tggaggacct tggacgccac cctgcccgca aagtcgttct
      421 gaaagaatcc caggctctgc cgcaggacat agcggtgcga aagccagcgt acgcgcgtct
      481 gcacagggcc agcgatcatc tggttctttg tgaggtcgtg cgcggtcgag accagcggcc
      541 tcagtatcag ggcgacgaac gccatgaata ccagcatgct gccattgtcg gaaatgaatg
      601 actgcggact ttgtgccgcc ttcatccgat cgacgatcgt gccgatgaac gcgtagagcg
      661 cgacctctat tagagagccg acgaggccaa cgatcagcag cagcagaaaa gcgggccaga
      721 cagggcggag gaaatgccaa tagaatgccc aaagcttgtc gggcgggcgt tccggcattt
      781 cctcaggaaa ggcgttgatg cgcgtttcga accagcggaa catctgtcac tttcgcgaat
      841 tgaggcggcg cgcgggtgca ggcccatgtt gaaaccggga cagcctggaa attagctggt
      901 ggggccaaca agcgctgacg gcagtttcaa gttctccggc gccggttgct tggagctcgg
      961 ttccgaagta gtcccaagcc acgtcacaag catggcctaa gggtacgaca tgctgcctca
     1021 gccggacgtc ctcgatcaat ggcgcgaacg cgcagcggcc tggttcgaaa cgctgcgtga
     1081 cgaaattcgg accgtattcg agcagctcga ggatgagctg cctccgggcg cgccgctttc
     1141 agatcgtcct ccaggccgtt tcgtcgcgac tccttggaac cggacggatc ataccggcgc
     1201 cccaggggga ggtggcgtca tgtcgatgat gagggggcgg gtgttcgaaa aggtgggcgt
     1261 gcacatatcc acggtgtttg gggagttcgc tccggagttc cgctcccaga tccctggcgc
     1321 gagcaacgac ccgcggttct gggcatccgg catctcactc atcgcgcata cgcaaaatcc
     1381 gcatgtgccg gctgcgcata tgaacacgcg gatggtggtg acgacgcggt ggtggttcgg
     1441 cggcggggcg gacttgacgc cgatgctcga ggcccgacgc acgcaatcgg acccggatac
     1501 gatcgccttc catgaagcga tgcgggcggc ctgcgctccg cacagcgtcg ccgactacgc
     1561 gcgattcaag gcctggtgcg acgaatactt ctatctgccg caccggaagg aaatgcgcgg
     1621 cgtcggcggg attttctacg attatctcac cccgaccgag gaggagggag gatgggaggc
     1681 ggcctttgcg ttcacgcaag atgtcggccg cgcgttccgc gatatttatc cgcagctcgt
     1741 gaggcggaac tgggaaaagc cttggaccga ggctgagcgc gaggagcagc tcgtccgccg
     1801 cggacgatac gtggaattca atctgcttta tgaccgcggc acaatattcg gcctcaagac
     1861 cggaggaaat gtcgacagca tcctgtcgtc gatgccgcct gtggtgaagt ggccgtgatc
     1921 caaagaggct gatgtggaag tccgcgaatc agggggccgg tagctccagg atcgccttga
     1981 gtccgccgta gcgtgagtcg gccaggtcca gtctgccgcg gtacaaatgc gcgaggtcgc
     2041 tgacaatgga aaggcccagt ccggacccag gctttgtttc gtccaggcgt aatccgcgct
     2101 ttccgatgcg gcggcgttga tctgtcgtca ggcccggccc gtcgtcctca attgtaacga
     2161 tcaaacgtga tcctccgccc cgctgcgaag gtggtttgat gacagcagag aaacggacat
     2221 ttttccgggc ccacttgcag gcgttatcca gcaagttccc gagcatctcc tcgagatcgt
     2281 gctgctcgcc ctggaaccgc gccgactcgg ggcagtcgac gagaatggca attcccttat
     2341 cgcgataaat gcgctcgagc gcacgctgaa gcgcctccac caccgggaga acctccgtca
     2401 ctcggacgag cgagcccgcg gtagccacct tcctcgcgcg atcgagatag tggttgatct
     2461 gatcacgcat caccttcgcc tgctccgcca ccttctgcgc gagcgggctc ttgtcgtccc
     2521 gggcctcatt cgtgatgaca gcgagcggcg tcttgagcgc gtgagccaga ttgccgacct
     2581 gggttcgggc gcgctcgatg atttcctcgt tcgactggat cagggcattg agttcgttct
     2641 gcagcggctc gatctccgcc ggaagctctc catccaagcg cgtcgctttt ccagacctga
     2701 tggccgccaa gccattctcg attgcacgga gtggcagcaa gccgaatcgc acctggatga
     2761 gcgtcaccgc aacaagtgct acgcctgcaa gggcgagggc catcgcgagc tgcgtaccaa
     2821 agcctgcgac ccgcgcatcg agccagtcga gcggacccgc aaccgagatc gagtactgcg
     2881 gcgcgtccgc gccttctccg aaagcgtaga tcgtctctgc aatgcgcacc cgctgatcgt
     2941 tggggcccag cccatccatc cagcgcgtgc cttggaggtc aggctcgacc cccgcttcat
     3001 agggggacgg cagaatgaac gtggcgaggg atggggaaac cttgatctcc ccgggcgcac
     3061 cccccaaagg tttgatctgc cagtaccagc cagaatgcgt gatgaagaat agaggctcgc
     3121 cgacctgtcc gggtgtgccg ggctgcgatt cggctccgtc gatggcatcg gcgtggacga
     3181 ttgtgaggag ctggctcaag cgttcgttga acccctcctc cacctcctgc cagtagaccg
     3241 aatagatcaa caggcccgcg accggcagga tcagcattgt cgagacaact gccgtcgcga
     3301 agagccgaaa ggcgagcgag ttaagcctca tctggctcct gagtgagccg gtaccccaga
     3361 ccgcgaacgg tcttgatcat gtcggccgga attttctttc gaagtcggcc aatgaaaact
     3421 tcgatcgtgt tgctgtcgcg atcgaagtcc tgatcgtaga ggtgctcgac gagctcggtc
     3481 cgcgagacga cccgctcgtt gtgatgcatg agataggcaa gtagccggta ttcgtgcgat
     3541 gtcagcttga ttggctggcc attgcaggtc acccgcgccg tccttgtatc gaggcgcagc
     3601 ggcccacact cgatctcact cttggcgtga ccagcaaatc gccgcacctg cgcccggact
     3661 cgcgccagca gctcctccat atggaaaggc ttggcaaggt agtcatctgc accggcatcg
     3721 atgccagcaa ccttgtcgct ccaacggtca cgcgcggtga gaatgatcac cggcatattg
     3781 cgacctgcgc ggcgccactg ctcgaggatg ctgatcccat ccatcttggg caggccgatg
     3841 tccaggatca cgatgtcgta aggctctgta tcgccgagga aaaagccttc ctcaccgtcg
     3901 tgggccgtat ccacggcgaa gcctgcatct gacagcgcct gcctgagctg gcgattcaga
     3961 tcaggatcat cttcaacaac gagcgcgcgc aatttctgga tccccccacg ggtcgagcca
     4021 gactattcgt ggtgtatacc ccaatcatgc gagccatcct agcgctcgga tggttgccgg
     4081 gcatcgagcg agatatgccg catgagcccg ccgggcaagc ggaccaccaa gcgatagaaa
     4141 tagcgctgcc cggcagaaca aagcgtagcc ttcacaatcg agccgccgag tgtggccctc
     4201 gcctgcttgc tcaattgctc gacggcgacc agccgctcct gcctgaccac cgcagccgcc
     4261 tcggaccagc tcttgaagca ctgattcgtt tcggctgtgg ccgtcgccaa atccgctaat
     4321 acgatcaggg caataagcgt tgtcgcgatc ctcagcatag ctcccagccc ctcaggagcg
     4381 attgtcatta gaactgaggg cctgaaccga acgtgaacgc gattagagct ggaaagtgac
     4441 ctgcttgcgt tcgagcgtgg ccgtagcgcg ggtccggcca tagattgtca tgatgattcc
     4501 gatcagaccg ccgattgcct gtaccacgag gaccacctgg tcgccaagct catggacgag
     4561 gtcgggtgtg atatcgattc cgaacaaggg tccgatcgtt ggaaggatgg ttgaggccgc
     4621 cgtgatgatc gccccccaga tcgtcatgga atgggcccac cacttgctgg tttgtgaagt
     4681 ctgatctgtc tgcgacactt ctgtctgcga cattggccgt gctcctgcct caggttgttg
     4741 ggaaatcttt gttccggcgc tcgctatttg gcgggccgct tcaactgtgc gatcgacccg
     4801 ccggagccat ccccgcccga aacgccagaa gtgaccaagg ctgcggtagc gcgcacgtcg
     4861 gatgtccgcg tagcgctcga ggacctcggc tatgggctgt ccatgcgcgg cggcgagcgt
     4921 ctggggtccg attactccgt cgacattcac ccccaaggcc tgctgcagca ttcgtgccgc
     4981 agcgccgaca ccatggttca ccgcggcatc gaagtgcatg acggcaagcg ccggcgggag
     5041 gtcgcctgcc cgggatggta gccagtatcg ctcccggtag atcggacgaa ccgcggcagc
     5101 atccaggcgc ttgagctcct c
//
LOCUS       DASUMG010000205         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1071026, whole genome shotgun sequence.
ACCESSION   DASUMG010000205 DASUMG010000000
VERSION     DASUMG010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071026"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VFX46_03385"
     CDS             complement(<1..835)
                     /locus_tag="VFX46_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated RND transporter
                     HpnN"
                     /protein_id="HEX5599658.1"
                     /translation="MGTSPTGRIVALSIRHPLIVLLAALALTAAAFVYMARNFAMTAE
                     TSQLISPKLEWRKRGIEFDKAFPQLQNLTMVVVDGVTPELADDAARRLAQALQSRPDL
                     FHNVRQPDGGRFFERNGLMLLPTEEVKSVTDSLIKVQPLLAALAADPSLAGAMKMLSV
                     TLLGVKDNSVKLKDIEPQLTALAATFEKAAAGEPAYFSWQTLVTGDSPDMLETRRLIL
                     VQPEMDYSQLQPGQGASDVIRKMAQSLDLDTAHGVRVRLTGQVPLADEEFASLADDAG
                     LI"
     gene            1102..1905
                     /locus_tag="VFX46_03390"
     CDS             1102..1905
                     /locus_tag="VFX46_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599659.1"
                     /translation="MRRLRQVPFWEEVLYFERRAGAIVAAFTETVADPVLKEALALQG
                     QEETRHAKLIRVMVDRYGLDTPERPIGDLGADIETSFKDFGFGECFDSFVGFGLFKIA
                     WQSEFLPREIMQIFSMLMYEETRHIVFFINWMAYTEARKGWLARTVSPLVTLRYYVRA
                     VHNKIRFARRGQQIDDGKGLAALQVSMFLDGFNFRRFLEDCYAENSRRMDVFDPELLR
                     PSFLPQLTAAALKALRLWGGRTDVEPKPSPLPPLYGAPPGSVAAQPPEG"
     gene            complement(1907..>2090)
                     /locus_tag="VFX46_03395"
     CDS             complement(1907..>2090)
                     /locus_tag="VFX46_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599660.1"
                     /translation="VVGVDLPNNTVSVIDGRGGPVRTYRADTIAKQDMLKKIKVGDVV
                     IGLSTPLMITAISPAN"
BASE COUNT          356 a          669 c          682 g          383 t
ORIGIN      
        1 tgatcaggcc cgcatcgtcg gccaacgagg cgaactcttc gtccgccagc ggaacctggc
       61 ccgtgaggcg cacgcggacg ccatgagcgg tatcgaggtc gagcgattgc gccatcttgc
      121 ggatgacgtc gctcgctccc tggcccggct gcaactggct gtagtccatc tcgggctgaa
      181 cgaggatcag ccgtcgtgtc tcgagcatgt cgggtgagtc gccggtgacg agcgtctgcc
      241 aggagaagta cgcaggctct ccggccgccg ccttctcgaa ggttgccgcc agtgccgtca
      301 gctgcggctc gatgtccttg agcttcacgc tgttgtcttt gacgccaagc agggtgacgc
      361 tgagcatctt catcgccccc gccaggctcg gatcggcggc gagcgccgcg aggagcggct
      421 gcaccttgat cagggagtcg gtcaccgact tgacctcctc cgtgggcaac agcatcaggc
      481 cgttgcgctc gaagaaccgg ccgccatccg gctggcgcac gttgtgaaaa agatcgggcc
      541 gcgattgcag cgcctgggcg agccgcctgg ccgcgtcatc ggccagctcc ggcgtcacgc
      601 cgtcgaccac gaccatggtc aggttctgca gttgcgggaa tgccttgtcg aactcgatcc
      661 cgcgcttgcg ccactccagc ttgggcgaga tcagctggct ggtctcggcc gtcatggcga
      721 aattccgggc catgtagacg aaggccgccg ccgtcagcgc gagcgcggca agcaacacga
      781 tcaggggatg ccggatgctg agcgcgacga tacgaccggt cggcgaagtg cccatggaca
      841 aaagcgcctc tgcggattgg tgcaagactg acccgggaac cagtaggcat tcgtggcgac
      901 tttgcaaccc catgccttgg gaggctgatg tgaatggaca aaacgggatt cgtggccatg
      961 aatgggccct ccgaccgcgg cgtgcctttg atgaaaatcg gatccccagc gcatcgcgat
     1021 gctttctgcg cgcacttcat gcgaaccact ctcgactatg atccagaggg gctgccctgg
     1081 cctgtcctgg acgaggcggc attgcgccgc ctccggcagg tgccattctg ggaggaggtc
     1141 ctctatttcg agcgacgtgc gggcgcgatc gtggcggcct ttaccgaaac cgtcgccgat
     1201 ccggtcctga aggaggcgct ggccctgcaa ggccaggagg agacccggca tgcgaagctg
     1261 atccgcgtga tggtggacag gtacggcctc gacactccgg agcggccgat cggggacctc
     1321 ggcgcggaca tcgaaaccag cttcaaggat ttcggcttcg gtgaatgttt cgactcgttc
     1381 gtgggtttcg gcctgttcaa gatcgcgtgg caatcggaat tcctgccccg cgagatcatg
     1441 cagatcttct cgatgttgat gtacgaggag acgcgccaca tcgtcttctt catcaactgg
     1501 atggcctaca cggaagcccg caagggctgg ctggcacgga cagtctcgcc gctggtgacc
     1561 ctgcgctact acgtgcgggc ggtgcacaac aagattcgct tcgcacgtcg tggccagcag
     1621 atcgacgacg gcaagggcct ggcggccctc caggtcagca tgttcctcga cggcttcaat
     1681 ttccggcgct tcctcgaaga ctgctacgcc gagaacagcc ggcgcatgga cgtcttcgat
     1741 ccggaattgc tgcgtcccag cttcctgccg cagttgacgg ccgccgcgct caaggcgctg
     1801 cggctgtggg gcgggcggac agatgtggaa cccaagccaa gtccgttgcc gccgctctac
     1861 ggcgcgcccc cgggttccgt cgccgcacag cctccggaag gctgacctag ttggccggcg
     1921 agatcgccgt gatcatgagc ggcgtcgaca ggccgatcac gacgtcgccg accttgatct
     1981 tcttcagcat gtcctgcttg gcgatcgtat cagcccggta ggtgcgcacc gggccgccgc
     2041 gcccgtcgat caccgacacc gtgttgttgg gcaggtcgac gccgaccacc
//
LOCUS       DASUMG010000206         4815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_26967, whole genome shotgun sequence.
ACCESSION   DASUMG010000206 DASUMG010000000
VERSION     DASUMG010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26967"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..600
                     /locus_tag="VFX46_03400"
     CDS             286..600
                     /locus_tag="VFX46_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5599661.1"
                     /translation="MINNMDVQKLGRESFDRAMRLLEDWNKGWQAIAAEMTDYSKRSI
                     EQGTKTFEKLLAAKSMEQAIEIQTNYAKRVYDDYMQQMTKLSNMYANLAKEAYKPLQP
                     DS"
     gene            872..1207
                     /gene="clpS"
                     /locus_tag="VFX46_03405"
     CDS             872..1207
                     /gene="clpS"
                     /locus_tag="VFX46_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX5599662.1"
                     /translation="MSNNGGPDRDSEEEGRTGLVTKTRPKTKRPSLYKVLLLNDDYTP
                     MEFVVHVLEKYFNKGREEATRIMLHVHHKGVGVCGVYTYEVAETKVTQVMEFARQHGH
                     PLQCTMEKE"
     gene            1215..3662
                     /gene="clpA"
                     /locus_tag="VFX46_03410"
     CDS             1215..3662
                     /gene="clpA"
                     /locus_tag="VFX46_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEX5599663.1"
                     /translation="MPSFSQSLEAALHRALEYANERKHEYATLEHLLLALIDDRDAAN
                     VMRACNVDLEALRARITDYLDNELKALEVAGPSEAQPTTGFQRVIHRAVVHVQSSGRE
                     EVTGANVLVAIFAERESHAAFFLQEQEMTRFDAVQYISHGIAKRPGMSEPRPARGVDE
                     DTTDGDGKKPGADALNTYCVNLNKKAREGRIDPLIGREAEVQRTIQVLCRRQKNNPLF
                     VGDPGVGKTAIAEGLARKIVRGEVPDVLKNATIFALDMGALLAGTRYRGDFEERLKAV
                     MKEIENYPGAILFIDEIHTVIGAGATSGGAMDASNLLKPALQSGTVRCIGSTTYKEYR
                     QHFEKDRALVRRFQKIDVNEPTIEDAIEILKGLKPYFEEYHKLKYTDDAIKAAVELSA
                     KYIHDRKLPDKAIDVIDETGASQMLLPEGKRKKKITVKEIEATVATMARIPPKTITKS
                     DAEVLANLERDLKRVVFGQDKAIEALAAAIKLARAGLREPEKPIGCYLFSGPTGVGKT
                     EVARQLAKLLGVELLRFDMSEYMEKHTVSRLIGAPPGYVGFDQGGLLTDGVDQHPHCV
                     LLLDEIEKAHPDLFNILLQVMDHGKLTDHNGKQVDFRNVILIMTTNAGAADLAKPPMG
                     FNRAKREGEDQEAISKLFTPEFRNRLDAIIPFAGLPPEVIYKVVEKFIFQLEAQLADR
                     GVTIELSEAATKWLAEKGYDDKFGARPLARVIQEYVKKPLADELLFGRLQHGGTVKVL
                     VTGEGAEQKLDFEIIPGEPKPPKASEEDEGDEDLEEDVEKALIEAAAPRKALPGPKDK
                     PVRRTTGTVPTVPRKTD"
     gene            complement(3711..>4815)
                     /locus_tag="VFX46_03415"
     CDS             complement(3711..>4815)
                     /locus_tag="VFX46_03415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5599664.1"
                     /translation="PHAHSWHLANAYIPMEVIAKLSELGVFGITLPEEYGGLGLGKEA
                     MCVVSEELSRGYIGVGSLGTRSEIASELILGSGTEEQKQRYLPRIASGEILPTAVFTE
                     PDTGSDLASLKTRAVREGDVYKVTGQKTWITHPVRADLMTLLVRTDPNEPGYKGLSIL
                     LAEKPRGTDEEPFPVPGMSGGEIEVLGYRGMKEYEISFDNFEVPVSQLLGGVEGQGFK
                     QLMATFESARIQTAARAVGVAQSALDHALAYARERIQFGRPIYAFPRVSNKIVMMAVE
                     IMIARQLTYYAARQKDKGRRCDLEAGMAKLLAARVAWSAADNAVQIHGGNGFALEYPV
                     SRILCDARILNIFEGAAEIQAQVIARRLLEGAN"
BASE COUNT          998 a         1444 c         1537 g          836 t
ORIGIN      
        1 cgcacgaccg gctcagaacc gccacacacg agctcgggct gttgcattcg catccaccga
       61 aaccagcggt ttttggcgcc tttgcggcgc tcagccgccg gccgatcatc agctcaaggc
      121 gggtggtgag cgtagggaag cgagactagg atctggttaa cgtctgccag taacagttgc
      181 ggcagcccca gggaacccga cttgacgtgg tatctgaggg gcgtatcttg tgcagcgcca
      241 actcgcgttg ctgccccgca gcccctcgaa agtcaggatt gcgtcatgat caacaacatg
      301 gacgtgcaga agctcggtcg agagagcttc gaccgcgcta tgcgtctttt ggaggactgg
      361 aacaaaggct ggcaggccat tgccgccgag atgacggact atagcaagcg ctcgatcgag
      421 caagggacca agacgttcga aaagctgctc gcagccaaga gcatggagca ggcgatcgag
      481 atacagacca actacgcgaa gcgcgtttat gacgactaca tgcagcagat gactaagctc
      541 agcaacatgt acgcgaacct ggcgaaggag gcttataagc cactccagcc ggacagctga
      601 tcgatgctgc ctggggggaa atgcgctcgt tgcatcgaat gaatcgacca gcgggctcgg
      661 caatggaccg ggcccttttt gtgtctgttt gccagctttg gcccaaagaa gagtggatcg
      721 tttcagtcaa acattgcgta tgatagtttg tgatccgggg cttgtcgtgc ctcaaacgcg
      781 acgtaacttt attctggtgc ttgggagtcc gaagaatagc agggacgctt cagacgcggg
      841 gcgtgtagag ccgatgacga agatcgtagc gatgtcgaac aacggtgggc ctgacagaga
      901 tagcgaggaa gagggccgca ctgggctcgt gacgaaaacc cggcccaaga cgaagcggcc
      961 gagcctctac aaggttctcc tgctgaacga cgactacaca ccgatggagt tcgtcgtcca
     1021 tgtgctcgag aagtatttca acaaggggcg ggaagaggcg acgcgcatca tgctgcatgt
     1081 tcaccacaag ggcgtcggtg tgtgcggcgt ctacacgtat gaagtggctg agaccaaggt
     1141 gacgcaggtc atggaattcg cgcgtcagca tggccatcct cttcagtgca ctatggagaa
     1201 ggagtaggtt ccccttgcca tcattctcac aaagtctcga agcggcgctc cacagagcgc
     1261 ttgaatacgc caacgagcgg aagcacgagt acgctacgct cgaacacctt cttctcgcac
     1321 tgatcgacga ccgggacgcc gcaaatgtga tgcgtgcttg caacgtcgat ctcgaagctc
     1381 tgcgagcccg catcacggac tatctcgaca atgagctcaa agctcttgag gtggcggggc
     1441 catccgaggc gcagccgacg accggcttcc agcgcgtgat ccatcgggct gtcgtccacg
     1501 tgcagtcctc cggacgtgag gaggtcacgg gggccaacgt gctcgtggcc atcttcgccg
     1561 agcgggagag ccatgctgct ttcttcctgc aggagcagga gatgacgcgg ttcgatgccg
     1621 tgcagtacat cagccacggc atcgccaagc ggcccggcat gtcggagccg cgtccggccc
     1681 gtggcgtcga cgaggacacg acggacggcg atggcaagaa gccgggcgcc gatgcgctga
     1741 acacgtactg tgtgaacctc aacaagaagg cgcgtgaggg ccgcatcgat ccgctgatcg
     1801 gccgcgaagc cgaggtgcag cgcacgatcc aggtcctttg ccgccggcag aagaacaacc
     1861 cgctcttcgt gggtgatcca ggcgtcggca agacggcgat cgccgagggt cttgcgcgca
     1921 agatcgtccg cggcgaggtc ccggacgtcc tcaagaacgc gaccatcttc gccctcgaca
     1981 tgggcgcgct gctcgcaggc acccggtatc gcggtgactt cgaggagcgg ctcaaggccg
     2041 tcatgaagga gatcgagaat tatccaggtg cgatcctctt catcgacgag atccacaccg
     2101 tcatcggggc cggcgccacc tccggtggcg cgatggatgc ctcgaacctg ctgaagccgg
     2161 ccctccagtc cggaaccgtg cgctgcatcg gctcaaccac ctacaaggag taccggcagc
     2221 acttcgagaa ggatcgtgcg ctcgtccgcc gcttccagaa aatcgacgtg aacgagccga
     2281 cgatcgagga tgcgatcgag attctgaagg gcctgaagcc gtacttcgag gagtatcaca
     2341 agctcaagta tacggatgac gccatcaagg ccgccgtgga gctgtcggcc aagtacatcc
     2401 atgaccggaa gctgcccgac aaggcgatcg acgtgatcga cgagacgggc gcctcgcaga
     2461 tgctgctgcc cgagggcaag cggaagaaaa agatcacggt caaggagatc gaggcgaccg
     2521 tcgccacgat ggcgcgcatc ccgcctaaga ccatcacgaa gagcgacgcc gaggttctgg
     2581 ccaacctcga gcgcgacttg aagcgcgttg tcttcggcca ggataaggcc atcgaagctc
     2641 tggccgccgc catcaagctg gcgcgtgctg gcctgaggga gccggagaag ccgatcggct
     2701 gctacctgtt ctcagggccg acgggtgtcg gcaagaccga ggtggcgagg caactcgcaa
     2761 agctcctcgg cgtcgagctg ctgcgcttcg atatgtccga gtacatggag aagcacacgg
     2821 tctcgcggct tatcggcgcg ccgcccggct acgtgggctt cgaccagggt ggcctgttga
     2881 ccgacggggt cgatcagcac ccgcactgcg tgctgctgtt ggacgagatc gagaaggcac
     2941 accccgatct cttcaacata ctcctgcagg tgatggatca cgggaagctg accgaccaca
     3001 acggcaagca ggtcgacttc cgcaacgtta tcctcatcat gacgacgaac gccggtgcgg
     3061 ccgatctggc gaagcctccc atgggcttca accgggcgaa gcgtgaaggc gaggaccagg
     3121 aggcgatcag caaattgttt acgcctgagt tccgcaaccg cctcgacgcg atcatcccgt
     3181 tcgccgggct gccaccggaa gtcatctaca aggtggtcga gaagttcatc ttccagctcg
     3241 aggcgcagct cgcggatcgc ggcgttacca tcgagctctc ggaggcggcg accaagtggc
     3301 tggccgagaa gggctacgac gacaagttcg gcgcccgtcc gctggcccgc gtgatccagg
     3361 agtacgtcaa gaagccgctt gcggatgagc tcctgttcgg caggctgcag cacggcggca
     3421 ccgtgaaggt gctggtcact ggagaaggcg ccgagcagaa gctcgacttc gagatcatcc
     3481 cgggtgagcc caaacctccg aaggctagcg aggaagacga gggcgacgag gacctcgagg
     3541 aggacgtcga gaaggcgctc atcgaggccg cagcgccccg aaaggccttg cctggtccga
     3601 aggacaagcc ggtccgcaga acgacaggca ccgtcccgac cgttcctcgt aagaccgact
     3661 gatcctttct gatccaccct tcccctaacg aggcgggaag ggtggacagc tcagtttgct
     3721 ccctccagca atcttcgcgc aatcacctgc gcctggatct cggctgcccc ctcgaagata
     3781 ttgaggatgc gggcatcaca gaggatgcgc gagacgggat actcgagcgc gaaaccgttg
     3841 ccgccatgga tctgcacggc gttgtccgcc gctgaccacg ccacgcgggc ggcgagcagc
     3901 ttggccatgc ctgcctcgag gtcgcatcgt cggcctttat ccttctgccg ggcagcgtag
     3961 taggtgagct ggcgggcgat catgatctcg accgccatca tcacgatttt gttggagacg
     4021 cgcgggaacg cgtaaatcgg gcggccgaac tgaatgcgct cccgagcata ggcaagggcg
     4081 tggtcaagcg cagattgggc gacgccgacc gcgcgggccg ccgtttggat gcgagcggat
     4141 tcgaaggtcg ccatgagctg cttgaatccc tggccttcga cgccgccgag gagctgtgaa
     4201 acagggacct cgaagttgtc gaaggagatc tcgtattcct tcatcccgcg atagccgagc
     4261 acctcgatct cgcccccgct catgcctggg acagggaacg gttcttcatc cgtcccccgc
     4321 ggtttttcgg cgaggagtat cgacaggccc ttgtagccgg gttcattcgg atcggtgcgc
     4381 acgaggagcg tcatcagatc ggcacgcacg ggatgcgtga tccaggtctt ctgccccgtg
     4441 actttgtaga catcgccttc ccgaaccgcc cgcgtcttga gagaggcgag atcggagccc
     4501 gtgtcgggct cggtgaatac ggccgtcggc agaatttccc cggaggcgat ccgtggcaga
     4561 tagcgttgct tctgctcttc ggtcccgctg ccgaggatca gctcgctcgc gatttccgac
     4621 cgcgtcccga gggagcccac gccgatgtat ccgcgcgaaa gctcctcgga gacaacgcac
     4681 atggcctcct tgccaaggcc gaggccgccg tattcctcgg gcagcgtgat gccgaacacg
     4741 ccgagctcgc tgagcttggc gatgacctcc atcgggatgt aggcattggc gaggtgccac
     4801 gaatgggcat gcggc
//
LOCUS       DASUMG010000207         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_990993, whole genome shotgun sequence.
ACCESSION   DASUMG010000207 DASUMG010000000
VERSION     DASUMG010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990993"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="metK"
                     /locus_tag="VFX46_03420"
     CDS             complement(<1..1103)
                     /gene="metK"
                     /locus_tag="VFX46_03420"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5599665.1"
                     /translation="MARRSYLFTSESVSEGHPDKVCDRISDEIVDLFFREADRTGFDP
                     WRVRVAAETLATTNKVIIAGETRGPETITHDLVAHVARMAIRDIGYEQPGFHWETADI
                     QVLLHPQSADIAQGVDSSDEKDEGAGDQGIMFGYACRETPELMPAPIYYSHRILQTLA
                     AARKAGEGDAAKLGPDSKSQVTVRYEGGKPVGVSQIVLSTQHLDPDLTSNQVREIVEP
                     YIRKTLPEGWIDSRTTWHVNPTGKFVIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFS
                     GKDPTKVDRSAAYAARYLAKNIVAAGLAERCSIQLSYAIGVAEPLSIYVDTYGTGQVD
                     EDKLEMAIRRVVTLTPRGIRKHLGLNKPIYARTA"
     gene            complement(1234..1731)
                     /locus_tag="VFX46_03425"
     CDS             complement(1234..1731)
                     /locus_tag="VFX46_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5599666.1"
                     /translation="MTRQVQAVADRDDEVVEKGSRRANPLDLHVGSRVRLQRMLLGMS
                     QEKLGEQLGLTFQQIQKYEKGINRIGASRLYELAKVLGVSVEFFYEDAPGTTPTNKSQ
                     NSSSGLAERQADRYVVDFLSSREGIELNKAFVKITDPKVRRAIVDLVRSLAGEEKEKE
                     RDKDA"
     gene            complement(1860..>3099)
                     /gene="lnt"
                     /locus_tag="VFX46_03430"
     CDS             complement(1860..>3099)
                     /gene="lnt"
                     /locus_tag="VFX46_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX5599667.1"
                     /translation="WRPGEERIIALALALSAAEWLRGHILTGFPWNVLGYALTWPLPL
                     MQSAGLIGIYGLTLAVILITAPPILALAKVASNPDRRRASLAIGILALLPLPVAWGYG
                     TIRLAEPQPPPVDGVRLRLVQPSVPQRDKWRPERQRDIFFDHVNLSQQRPDGSFDGLS
                     GVTHVIWPEAAMPFLPLETPAALEAIANMLPEGTILLAGALRADGTTDAELGRRRAFN
                     SLIALNSDGQPVGIYDKVHLVPFGEYLPLQPVLERLGLQKLVGWRGGFSSGQSPRPLF
                     DVPGLPPVGALICYEAIFPGAVVQSASRPQALINVTNDGWFGLTTGPHQHFHQARVRA
                     VEEGLPLIRVANNGISGVIDANGRVLARLDLDERGVIDSPLPGAKNPTVYARYGDVVF
                     GVLWLCGLICLRILQRRKTQ"
BASE COUNT          582 a          945 c          940 g          632 t
ORIGIN      
        1 gccgtccggg catagatggg cttgttcaaa ccgaggtgtt tgcggatgcc gcggggcgtg
       61 agcgtgacaa ccctgcggat cgccatttcc agcttgtcct cgtcgacctg gccggtgcca
      121 taggtgtcta cgtaaatcga cagtggctcg gcgacgccga tggcgtagga aagctggatg
      181 ctgcagcgtt ccgccagccc cgccgcgacg atgttcttgg cgaggtaccg cgcggcgtaa
      241 gcggccgacc ggtcgacctt ggtcgggtcc ttgccggaga atgcgccgcc accgtgcggg
      301 gccgcgccgc cataggtatc gacaatgatc ttgcggcctg tgagcccgca atcaccgtcc
      361 ggtccaccga tcacgaactt cccggtcgga ttcacgtgcc aagtggtccg cgaatcgatc
      421 cagccttccg gcagggtctt gcggatgtaa ggctcgacga tctcccgcac ctggttggag
      481 gtgaggtcgg ggtcgaggtg ctgggtcgag agcacgatct ggctcacccc gaccggcttc
      541 ccgccctcgt agcggacggt cacctggctc ttggagtccg gcccgagttt tgctgcgtcg
      601 ccttctccgg ccttgcgggc cgcagctagc gtctgaagga ttctgtggct gtagtagatc
      661 ggtgccggca tcagctccgg ggtttcgcgg cacgcgtacc cgaacatgat cccctggtcc
      721 ccggcgcctt cgtccttctc gtcggagctg tcgactcctt gagcgatatc agcggattgc
      781 gggtgcagca gcacctgtat gtccgccgtt tcccagtgaa agcctggctg ctcgtacccg
      841 atgtcccgga tggccatccg ggccacatga gccacgagat cgtgcgtgat tgtttcgggg
      901 ccgcgcgtct ctcccgcaat gatgaccttg tttgtggtcg ccagcgtctc ggcggctacg
      961 cgcaccctcc agggatcgaa gccagtccga tcggcctcgc gaaagaaaag gtcgacgatc
     1021 tcgtcggaaa tccggtcaca gaccttgtcc ggatgcccct cagagacgga ttcgctagtg
     1081 aaaaggtacg atcggcgtgc cacgagtatc cccgtaaagc tgttgaccgc aagtgatggc
     1141 gcttctttac agcctttggg gggcgaatca agcgcgtcta agacgccggc caagcctttg
     1201 cccgcaaacc tggccggcgt gcgcttgttg acgctaagcg tccttgtccc tttctttttc
     1261 cttctcctcg cccgcgaggc tccggacgag atcgacgatc gcacgccgaa ctttcgggtc
     1321 ggtgattttc acgaacgcct tgttcagctc gatgccttcc cggctgctga ggaaatcgac
     1381 gacgtaccga tcagcctgcc gctcggctag cccagacgat gagttctgag atttgttggt
     1441 tggcgttgtg cctggggcgt cctcgtagaa gaactcgacg ctgacaccca gcactttcgc
     1501 gagttcgtaa aggcggctgg caccaattct gttgatgccc ttctcgtatt tctgaatctg
     1561 ctggaaggtg agaccgagtt gctcgccgag cttttcttgg ctcattccga gcagcatgcg
     1621 ctggaggcgc acgcgactgc cgacgtgaag atcaagcgga ttggctctcc ttgagccttt
     1681 ctcgactact tcatcatcac gatcggcaac agcttgcact tgtctggtca tgcccctagt
     1741 ccccaccctt gtgactggat tttttacagc gcaattcgta ggtgcaggta aagctgccgt
     1801 caaccggaac aagaggtgcg gaagctccct tctaccgcgc tttctgcttg gttatcagct
     1861 tactgcgtct ttctgcgttg aagaattcgt aggcagatta atccacatag ccaaagaacc
     1921 ccgaaaacaa catcgccgta ccgagcgtac acggtcggat tttttgcgcc ggggagtggc
     1981 gaatcaatca caccccgttc gtcaagatcc agccgcgcga gaacgcgacc gttggcatca
     2041 attacccctg atatcccgtt attggcaacg cgaatgagtg gcagcccttc ttcgactgcc
     2101 ctgacgcgag cctgatgaaa gtgctggtgc ggaccggttg tgaggccgaa ccagccgtcg
     2161 ttggtgacat tgatcagcgc ctgcggccgg ctcgctgatt ggaccacagc gcccggaaaa
     2221 atcgcttcat aacaaatgag tgcgccgacg ggcggcagtc ctggaacatc aaaaagaggg
     2281 cgcggtgatt gtccggatga aaagccgccg cgccagccca cgagcttctg caacccgagc
     2341 ctctcaagca cgggctgcag gggcaaatac tcaccgaacg gaacaaggtg caccttgtcg
     2401 taaatgccga ctggctgacc gtcagagttc agagcaatga ggctgttgaa ggcgcgtcgt
     2461 ctgcctaact ccgcatccgt cgttccgtcc gctctcagag cacctgcgag caagatggtg
     2521 ccttccggca acatattcgc aatcgcttcc aatgcagccg gcgtttcgag cgggaggaac
     2581 ggcatggccg cctccggcca tatgacatgc gtgacgccgg agaggccgtc gaagctgccg
     2641 tcaggccgct gctgcgacaa attgacgtga tcgaagaaaa tatcccgttg ccgctcaggg
     2701 cgccacttgt ctcgctgcgg aacgctcggt tgcacaaggc gcagacgaac accatcgacc
     2761 ggtggcggct gcggttcggc gagccggatc gtcccgtagc cccacgccac cggcaatggc
     2821 agcaaagcca agatgccgat ggcgaggctc gcgcgtcgcc ggtccggatt ggaggctacc
     2881 ttggccagtg ccaggatcgg cggagcggtg atcaaaatga ccgcaagggt caggccatag
     2941 attccgatca aacctgcgga ctgcatgagc ggcagcggcc aggtgagcgc gtagccgagc
     3001 acattccaag gaaagcctgt caggatatgg ccgcgaagcc attcagccgc ggagagtgcc
     3061 agcgcaaggg cgataattct ctcctcgccg gggcgccag
//
LOCUS       DASUMG010000208         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1071126, whole genome shotgun sequence.
ACCESSION   DASUMG010000208 DASUMG010000000
VERSION     DASUMG010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071126"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="VFX46_03435"
     CDS             <1..1259
                     /locus_tag="VFX46_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX5599668.1"
                     /translation="MGQPLVIEATATNEGTLLAEIGRLMTAAEQGRGRYVRLADRAAR
                     LYAPAVHLLGLLTFIGWMLLGHGWEAALTAAIAVLIITCPCALALAVPAVQVAASSRL
                     FGQGVLVKAADGLERLSEVDTIIFDKTGTLTMGEPRLVDPQSCSDELLNRAAALAAAS
                     RHPYARAVVKEARARGLDPVAATNVREVAGAGLACETEHGEERLGSAAWCGVGSETGD
                     LATLWFKPAEGDPIALRFEDHLRPDAAEVISTLKKAGYRIELLSGDRPAAVEAAAREA
                     GIEHWHARQNPAQKIARLEALKAEGRKVLMVGDGLNDAPALAAAHASLSPSSAADISQ
                     TAADLVFQGERLGAVVEALAVAKASHRMALQNFGLSLAYNAVFVPLAVAGYATPLIAA
                     IAMSSSSIAVTANAVRLRSKRLKLKV"
     gene            1263..1439
                     /gene="ccoS"
                     /locus_tag="VFX46_03440"
     CDS             1263..1439
                     /gene="ccoS"
                     /locus_tag="VFX46_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEX5599669.1"
                     /translation="MTALAWLIPAALFLGGLGLAAFLWALNTNQFEDLDGAAWRALQD
                     DELTTPTGEGSDPR"
     gene            complement(1482..2291)
                     /locus_tag="VFX46_03445"
     CDS             complement(1482..2291)
                     /locus_tag="VFX46_03445"
                     /inference="COORDINATES: protein motif:HMM:NF021472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HEX5599670.1"
                     /translation="MSNQTVQAGTISTRLAAAHIELRRVPFDAPWNWLAAGWRDLWSA
                     PHVGLTYGSAFAIAAFVLTAGLLQFGMQSLILALAGGFLLIGPLVAVGLYEASRRLET
                     GKPVALRDVMFAGLRAPGQLGFFGVVLAFAYAVWVQLAFLMFMLFMGSRGLPPASEFV
                     PTLLFTPHGLGLLVAGTAVGGVLATIIFSVSVISVPLLLTRPIDAVTAMAASIEAVVK
                     NPKPMALWAALIAGFMILGTLTLFVGLAVAFPLIGHATWHAFRSLVVQNGA"
     gene            complement(2463..>2665)
                     /locus_tag="VFX46_03450"
     CDS             complement(2463..>2665)
                     /locus_tag="VFX46_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5599671.1"
                     /translation="RHGVNVSVDIVSAPQSSAAEEIQRRTEKSELLVMGAYGQSRFRE
                     LVFGGVTRHLLRNMTIPVLFSH"
BASE COUNT          521 a          829 c          878 g          437 t
ORIGIN      
        1 acatgggcca gcccctggtg atcgaggcga cggccaccaa cgaaggcacg ttgctcgccg
       61 agatcggacg gctgatgacg gccgccgagc agggccgggg gcggtatgtg cgcctcgccg
      121 atcgggcagc gcgcctctat gcgcccgcgg tccacctgct gggccttttg acgttcattg
      181 gctggatgct cctcgggcac ggctgggagg ccgcgctgac ggcggccatc gcagtgctca
      241 tcattacctg tccgtgcgcg ctggctttgg cggttcccgc agtccaggta gctgcgagca
      301 gtcggttgtt cggccagggc gtgctcgtca aagcggctga cggtctcgag cggttgtccg
      361 aggtcgatac aatcatcttc gataagaccg gcacgctgac catgggcgag ccacggcttg
      421 tcgatccgca atcttgtagc gacgagctct tgaaccgtgc ggccgccctc gcggcggcca
      481 gccgccaccc ttacgcgcgg gccgttgtca aagaggcgcg cgcccgcggt ctcgatccgg
      541 tcgcggcaac caacgtgcgg gaagttgcgg gcgcgggtct cgcctgcgaa acggagcacg
      601 gcgaggaacg gctcgggtcc gcagcctggt gcggggtcgg cagcgagacc ggcgatctgg
      661 ccacgctttg gttcaagcct gccgagggtg atcccattgc gcttcgcttc gaggaccacc
      721 tgcggccgga tgcggcggaa gtcattagca ctctcaagaa agcgggttac cgcatcgagc
      781 tgctttccgg cgaccggccc gcagccgtgg aagcggcggc gagagaggct gggatcgagc
      841 actggcacgc ccggcagaac ccggctcaga agatcgcacg gctggaagcg ctcaaggcgg
      901 aaggccggaa ggtgctgatg gttggcgatg gtttgaacga cgctccggcg ttggcggcgg
      961 ctcatgcttc cctgtcgcca tcttccgctg cggatatcag ccagacggcg gcggatctcg
     1021 tgttccaggg cgagcggctg ggggccgtcg tcgaggcgct agccgttgcc aaggcgtccc
     1081 accgcatggc cctgcaaaac ttcggcttgt cgctggcgta taatgccgtt ttcgtgcctc
     1141 tggcggtggc aggctacgcg acgccgctca ttgccgcgat tgccatgtcg agctcgtcta
     1201 ttgctgtgac ggccaatgct gttcgcctca gatcgaagcg cctgaagctg aaggtgtaac
     1261 ccatgacggc tttagcttgg ctcatccctg cagcactgtt cctgggcggc ctcgggctcg
     1321 ccgcctttct ctgggcgctg aacacgaacc agttcgagga tctggacggg gcagcttggc
     1381 gggcgcttca ggacgacgag ctcacgacac ccactggaga aggatcggac ccccgctaag
     1441 acgctagaaa gagggcagct cgaattatcg aaaagaagct gtcaggcccc gttctggacg
     1501 acgaggctcc ggaacgcatg ccacgtggca tggccaatta acgggaacgc gacagccaga
     1561 ccaacgaaca gggtcagcgt gcccaggatc atgaagccag cgatcagcgc agcccaaagg
     1621 gccattggct tcggattctt tacgaccgcc tcgatgctcg ctgccatcgc cgtcaccgca
     1681 tcgatcggtc tggtaagcag gagcggcacc gaaatgacgg acaccgagaa aatgatggtg
     1741 gccagcacgc cgccgaccgc agttcctgca acgagcaggc cgagtccgtg gggcgtgaac
     1801 agcagcgtgg gcacgaactc acttgccggc ggcagaccgc gactgcccat gaacagcatg
     1861 aacatcaaaa acgcgagctg cacccagacg gcataggcga aggccaggac gacgccaaaa
     1921 aagccaagct gtccgggagc cctgaggccg gcgaacatca cgtctcgcag cgccaccggc
     1981 ttccctgttt ccaaccgccg gcttgcctcg tacaggccaa cggcaacgag ggggccgata
     2041 agcaagaagc caccagcgag ggccaggatc agggactgca tgccgaattg cagcaagccg
     2101 gcggttagca cgaaagctgc gatggcgaag gcagagccgt aagtcaggcc cacatgcggg
     2161 gcgctccaaa ggtcgcgcca gccagcagcc agccaattcc acggcgcgtc gaaaggaacg
     2221 cgccgcaact cgatgtgggc tgccgcgagc cgtgtactga tggtgcctgc ttgaaccgtc
     2281 tgattggaca tgcgctggac cacccccgca cctcatcgta agggcccccg cgctggcggc
     2341 cgagtgtccg cgcaacgcgt aaggacagct ctcaacatgc atccgttcaa atggagataa
     2401 acttattcaa tcgatttctt gaaacggatg caacgaggaa cgtacgcaag agcctccgca
     2461 ggtcagtgcg agaacaggac agggatggtc atgttccgaa gcaggtggcg ggttacgccg
     2521 ccgaacacga gctcacggaa gcgcgattgc ccgtaggcgc ccataaccag cagctccgat
     2581 ttctcggtgc ggcgctggat ttcctctgcg gctgacgact gtggagccga aacgatgtcg
     2641 acagagacgt taaccccatg ccggt
//
LOCUS       DASUMG010000209         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_107785, whole genome shotgun sequence.
ACCESSION   DASUMG010000209 DASUMG010000000
VERSION     DASUMG010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107785"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFX46_03455"
     CDS             <1..529
                     /locus_tag="VFX46_03455"
                     /inference="COORDINATES: protein motif:HMM:NF022400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEX5599672.1"
                     /translation="CSQTNRTGTVQTYLVYEPPNPPKELIKRAERMVFVREGFSWMAA
                     LFTPLWLLFNRMWLALLLYVIAMVALSVAFVAADLEQQWLTIASLAVHLAIGFEAGSL
                     RRWSLERRRWRMLGAVVGPSLLECERRFFQAWLGQSTGQDRESVRLARTEEQTSAQSP
                     RPIIEPSVNPVGSEA"
     gene            540..1193
                     /gene="hisH"
                     /locus_tag="VFX46_03460"
     CDS             540..1193
                     /gene="hisH"
                     /locus_tag="VFX46_03460"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX5599673.1"
                     /translation="MQRVTIIDYGSGNLHSARKAFERAALEADLSVKIEVTARPEDVR
                     SADRIVLPGVGAFADCKRGLDGVAGMSEALEDAVRKDGRPFLGICVGMQLMATRGLEF
                     GETPGLGWIEGEVKAIEPADRSLKIPHMGWNTLTVVNDHPLLTGIRTGADGLHAYFVH
                     SYHLVPRDRSVVVAETDYGGPITAIVAKGNTAGTQFHPEKSQVLGLKLIANFLRWRP"
     gene            complement(1252..1608)
                     /locus_tag="VFX46_03465"
     CDS             complement(1252..1608)
                     /locus_tag="VFX46_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599674.1"
                     /translation="MRLFLISYDLARSLRNKHAIATAIMNLSQAWARPLETTWYVRTN
                     ASDDDIQAELAGLLDSEDGLIVQAVSEAALLVNTSLRWFRRRRPVAEALGSILAFPVN
                     APSAIAQPELQLAQAG"
     gene            complement(1764..2081)
                     /locus_tag="VFX46_03470"
     CDS             complement(1764..2081)
                     /locus_tag="VFX46_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599675.1"
                     /translation="MTAPSAPSDPVPGNAGHRRALTEADAIDVWIARWLRARPIDLCR
                     RYQCDPRRLYEIWEGQRFPAAREKAWQIFQERYPGLVDRVNPGLHRRMPRGRHPDQLS
                     LFD"
     gene            2195..2479
                     /locus_tag="VFX46_03475"
                     /pseudo
     CDS             2195..2479
                     /locus_tag="VFX46_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
     gene            complement(2457..2780)
                     /locus_tag="VFX46_03480"
     CDS             complement(2457..2780)
                     /locus_tag="VFX46_03480"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5599676.1"
                     /translation="MLVSVPHYGTEPLPHITRDDYGEARFETFAYGFADTFARDVYGD
                     LHDHGVTVVATPVSRMFVDVNRRRDDFEHHEGEVRSRRGVVRTHTMREVPIFARPLPC
                     RATFQ"
BASE COUNT          509 a          855 c          921 g          536 t
ORIGIN      
        1 ctgcagccag acgaaccgga cgggcaccgt gcagacgtat ttggtttacg agccgcccaa
       61 tcctcccaag gagctgataa agcgcgcgga gaggatggtt ttcgtgcgcg agggcttctc
      121 gtggatggcc gctctgttca cgcccctctg gcttctcttc aaccggatgt ggctcgccct
      181 acttctctac gtcatcgcga tggtggcctt gagtgtggct ttcgtcgcag ctgatctcga
      241 acaacaatgg ctcacgatag cgagcctggc tgtgcacctt gcgatcggtt tcgaggcagg
      301 gtcgctacgc cggtggagcc tggaacgccg ccgttggcgc atgttggggg ccgtcgtcgg
      361 gccatctttg ctcgaatgcg agcgccgctt cttccaagct tggctgggcc aaagcactgg
      421 gcaagacaga gaatcggtgc gcttggcaag gaccgaggag cagacctcgg cgcaatcgcc
      481 tcggcccatc atagagccca gcgttaatcc cgtcggctca gaggcatagc aggccgccga
      541 tgcaacgcgt caccatcatt gactacggtt cgggaaacct gcattcggcc cggaaagctt
      601 tcgagcgcgc ggcgttggag gctgaccttt ccgtcaagat cgaggtgacc gcgcggccgg
      661 aagatgtccg ctcggcggat cggatcgtgc ttccgggcgt cggagctttt gcagactgta
      721 agcggggcct cgacggcgtt gccggcatga gcgaggcgct cgaggatgcg gtcaggaagg
      781 atggccgccc atttctaggc atctgcgtcg gcatgcaact catggcgacc cgcggcctcg
      841 aatttggcga gacacccggg cttggttgga tcgaaggcga ggtcaaggcg atcgagcctg
      901 cggatcgatc tctgaaaatc ccgcacatgg gttggaacac gctcacagtc gtcaacgatc
      961 atcccttgct tacggggatt cgcaccggag ctgacggcct gcacgcctac ttcgttcatt
     1021 cctatcatct cgtgccgcgt gatcgcagcg tcgtggtggc cgaaactgat tacggcggcc
     1081 ccattactgc gatcgtggcc aagggcaata cggccgggac gcagttccat cctgaaaaga
     1141 gccaagtcct tggcctgaag ctcatcgcga atttcctgcg ctggcggccc tagttcgcag
     1201 cgagtcgccg cgcctgcgaa tgctcccttc ccgccgggct tcgacgggtc cttatcccgc
     1261 ctgagccagc tgcaactcgg gttgtgcaat cgctgaggga gcattgactg ggaacgcgag
     1321 aatgctgccc aaagcttcgg caacagggcg gcgccgacgg aaccagcgga gggatgtgtt
     1381 gaccagcaag gccgcttcgc tcacggcctg gacaatgagg ccgtcttccg aatccaacag
     1441 tcccgccagc tcagcctgga tgtcatcgtc agaggcattg gtgcgtacgt accacgtcgt
     1501 ctcgagtggc cgcgcccagg cctggctgag attcatgatg gccgtggcga tcgcgtgctt
     1561 gttccggagc gatcttgcga ggtcatagga gatgaggaag aggcgcattg gtcgggtctc
     1621 ctagcgagag cggagcggta cgcgagacct gagaccgcga atggatcggt taatagaacg
     1681 taaagagaac atgccgcgaa tcaccggcgg tgtccagtag aaagttctct ctttgttcgc
     1741 cctagccgct tgaagagatc agcttagtca aacaacgaga gctgatccgg atgccggccg
     1801 cgcggcatcc gccggtgcag gccggggttg acgcggtcca ctagacctgg gtagcgctcc
     1861 tgaaagattt gccatgcttt ctcccgcgct gccgggaagc gctgtccttc ccagatctcg
     1921 taaagccgtc gcgggtcgca ttggtaccgg cggcaaagat cgatcggccg cgcacgcaac
     1981 cagcgcgcaa tccacacgtc gatggcgtcg gcctcggtca aagctcggcg gtgaccggcg
     2041 ttcccgggaa ctggatcgct cggcgcggat ggagcagtca ttcgcggatg cttgacgcaa
     2101 cgacttgttt cattccttta tcgcgcgtga tcatagccga catatggttc gcgcgggcag
     2161 atcgcgcgct gactcaccca ctcgagacga agcattgatc ctcttccccg ccatcgacct
     2221 taaggacggc cagtgcgtcc ggctgaagct cggcgagatg gacgcggtga ccgtcttcag
     2281 tgacgatccc gccgcccaag cccgaacctt cgaggaacag ggcttccagt ggctgcacgt
     2341 ggtggacctg aacggcgcct tcgcgggtaa gccggtgaat gccgatgccg tcgaggcgat
     2401 tcttggcgcc gtatccattc cggtgcagct cggaggcggc attcgcgatc tggccactat
     2461 tgaaatgtgg ctcggcaagg aagcgggcgg gcgaagatcg gtacctcgcg catggtgtgg
     2521 gtgcgcacca ccccccggcg cgagcgcacc tcgccctcgt ggtgctcgaa gtcgtcgcgg
     2581 cggcggttga cgtcgacgaa catgcgcgag accggcgtcg ccacgaccgt gacgccgtga
     2641 tcgtgcaggt cgccgtacac gtcgcgcgcg aacgtgtccg cgaagccgta ggcaaacgtc
     2701 tcgaagcggg cctcgccgta gtcgtcgcgt gtgatgtgag gcaacggctc ggtgccgtag
     2761 tgaggcacgc tgaccagcac cggcgtgcgc gctcccggcg ccgggtgcct gacgatcggg
     2821 a
//
LOCUS       DASUMG010000210         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1151927, whole genome shotgun sequence.
ACCESSION   DASUMG010000210 DASUMG010000000
VERSION     DASUMG010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1151927"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /gene="lptG"
                     /locus_tag="VFX46_03485"
     CDS             complement(<1..937)
                     /gene="lptG"
                     /locus_tag="VFX46_03485"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEX5599677.1"
                     /translation="MRALTLKLYIARHFVIGILGTFLVCALLIFMIDFIEVLRQSGKR
                     GSVPAATLVWLTLLRLPAYTEILLAFSVLVGSIASLLHLSRRSELAVIRAAGMSVWQF
                     LSPGIVVAFILGVLAVTFYNPLAAKARAEAEELFAEVFGKESNFLKTRAGSWLRQDGA
                     EGSSVMNAGGVADGGLRLFTVTVFQYDREGRFVERIEAKRATLREGYWELEDVRVAGV
                     GREPQRYARYLISTYLTPERVQDALGSIISLSFWELPGLIEVVERAGLSAAPYRIQYE
                     LLLSRPLLLICMVLFAATVSLRSFRSGGIQTMVATG"
     gene            complement(944..2152)
                     /gene="lptF"
                     /locus_tag="VFX46_03490"
     CDS             complement(944..2152)
                     /gene="lptF"
                     /locus_tag="VFX46_03490"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEX5599678.1"
                     /translation="MNTFSRYLFRQVAGALLLILLSLAGVVWIALALRELELVTTEGQ
                     NAWVFVIMTTLALPNLLGLIAPIALLIATIHTLNRMSGDSELIVLTASGASIWTLARP
                     LLLLALLVAIGVAFVNHIGMPWSLQMLRAYAIEVHTDLISKVLQPGQFSSPERNLTIH
                     IRDRTYNGELRGILLQDARNAEQTMAYLADRGVLSKQDESTFLVMFDGHIVRKTADGD
                     APQIITFESYAIDLNRFEPKSEGAVLKPRERYFSDLAWPDPDDPYARHRPGHLRAELH
                     ERLSSPLYPFAFVMIALAFVGQARSTRQGRMEGLIMAFVVAAMIRLGGLAVNNLVAIR
                     ASTVPLLYLLPLGGIVLSYVVIRMNAQPRGGATVSDRLAQVVSALRAMCAPWAPARSV
                     VRAARRQGAR"
     gene            2466..>3655
                     /locus_tag="VFX46_03495"
     CDS             2466..>3655
                     /locus_tag="VFX46_03495"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX5599679.1"
                     /translation="MSEPLAIEFAPLKAPLQHVAALLCADKLALGPQTRALNGKANGA
                     ILGAAEAVEFTGKAKTSFELLTPAGLDVRRLVVIGAGQPDKAKETDWVNLGGYAYAQI
                     TARKTEVASLVAEVADPRTRSAADIAADMAFGAVLRSYRFQKYITRQREDENGERKPD
                     GLTRLIVHTQDPDAAATAFARRKAVASGIFLARDLVNEPANVLGPVEFAERVQELSRV
                     GVEVEILDADALKELKMNALLAVSQGSVRPPRVAVMQWNGAKSRRSKPLCFVGKGVCF
                     DTGGLSMKPAQGMEDMKGDMGGAACVAGLMKTLAERQANVNVVGLVGLTENMPSGTAQ
                     RPGDIITSMSGQTIEVLNTDAEGRLVLADVLWYAQQRFKPRFVIDLATLTGAILIALG
                     KEHAG"
BASE COUNT          741 a         1151 c         1174 g          589 t
ORIGIN      
        1 ttccggtagc aaccatagtc tgaatgccgc ccgaccggaa tgaccgcaac gacacagtcg
       61 cggcaaaaag aaccatgcat atcaatagaa gaggccgcga cagcaggagc tcgtactgga
      121 ttctgtacgg ggcggctgac agcccagcgc gctcgacgac ctcgatgagg cccggaagct
      181 cccagaacga caaggaaatg atgctgccga gagcgtcttg aaccctctcc ggggttagat
      241 acgtgctgat cagatagcgc gcatagcgct gaggctcgcg cccgacgccg gcgacccgta
      301 cgtcttccag ctcccagtag ccttcgcgca gggtcgcccg tttcgcctcg atgcgctcga
      361 cgaagcgccc ctcgcggtcg tactggaaga cggtgacggt gaagagccgc aggccaccat
      421 cggcgacgcc acccgcgttc atcaccgagg agccctcggc accatcctgg cgcagccacg
      481 acccggccct ggtttttaga aagttcgatt cctttccgaa tacctccgcg aacagctcct
      541 ctgcctcggc gcgagccttg gccgcgagag ggttgtagaa ggttaccgcc agcacgccga
      601 ggatgaaggc aacgacgatc ccgggactga ggaactgcca aacggacatg ccggccgcgc
      661 gaatgactgc gagctcgctg cggcggctca aatggaggag gctcgcgatg ctgccgacga
      721 gcacggagaa ggcaagcagg atctcagtgt aggccggtag ccgcaagagc gtcagccaga
      781 cgagagtcgc cgccggcacg cttccgcgct ttcccgactg gcgcagcacc tcgatgaagt
      841 cgatcatgaa gatgagcagg gcgcagacga gaaacgtgcc cagaatgccg atcacgaagt
      901 gccgcgcgat gtagagcttg agcgtcaacg cgcgcatgct ggatcacctc gccccctgcc
      961 gacgggcagc tcgaacgacc gaacgcgccg gagcccaggg ggcgcacatt gcgcgcaggg
     1021 cggatacaac ttgggcaagc cgatccgaaa ccgtcgcccc tccgcgcggc tgcgcattca
     1081 tccggatcac gacataggag aggacgatcc cgccgagcgg cagcaggtag agcaggggga
     1141 ccgtcgaggc acggatcgca accaggttat tgaccgccag gccacccaga cggatcatcg
     1201 ccgccacaac aaaagccatg ataaggcctt ccatcctgcc ctgccgcgtc gagcgagcct
     1261 ggccgacgaa cgccagggcg atcatcacga aggcgaacgg atagagcggg ctggaaagcc
     1321 gctcatgcaa ctcggcgcga agatggcccg gccggtggcg agcatagggg tcatctggat
     1381 caggccaggc gaggtcgctg aaatagcgct cgcggggctt cagcaccgct ccctcgctct
     1441 tcggctcgaa gcgattgaga tcgatggcat agctctcgaa agtgatgatt tgcggggcat
     1501 cgccgtcggc ggtcttccgg acgatgtggc catcgaacat aacgaggaag gtcgactcgt
     1561 cctgcttcga cagcacgcca cgatcggcga gataggccat ggtctgttcc gcgttccgcg
     1621 cgtcctggag caggatgccg cgcagctcgc cgttgtaggt ccggtcccgg atgtggatcg
     1681 tgaggttgcg ctccgggctc gagaattgcc ccggctgcaa cactttggag atgaggtccg
     1741 tatgcacctc gatggcatag gcgcggagca tttggaggct ccacggcatg ccgatgtggt
     1801 tgacgaacgc gacacctatc gcaaccagca gtgcgagaag gagcagaggg cgcgccaagg
     1861 tccagatgct cgccccggat gccgtcagca cgatcaactc gctgtcgccg ctcattcgat
     1921 tgagcgtgtg gatcgtggcg atcagcagag cgatgggcgc gatcagcccg agcaggttag
     1981 gcagagctag ggtcgtcatg atgacgaaca cccaggcgtt ctgcccttcg gttgtgacga
     2041 gctcgagctc tcgcagggcc agcgcgatcc agacgacacc ggcgagggac aacaggatca
     2101 gcagtagcgc ccccgcgacc tgccggaaca gatagcggct gaaggtgttc atggccaacc
     2161 agcctcccca agccgctcgg acttctcgaa agcttctgcc cccaccgtat cgctgctccg
     2221 tgctccgccc gttcgcccgc ggtctcatag cacaatggcg gaaattcgac tccacgccgg
     2281 cgccggttct tgtcaaatca agcctgtggc ctcgtgacct cgcttgcact tctcgcaggg
     2341 gcgactaagt tcgcccggat tgcactgcga cgcgatcctc ccaccagtcc tcgagaggtc
     2401 gattgggcag acctgcaact cgaggccgtc cggtgccgta gctcaggacc agtccaggac
     2461 gaatcatgtc agagcctctt gccatcgaat tcgcgcccct caaggcgccg ctgcaacacg
     2521 tggccgccct actatgtgcc gacaagttgg cgttgggccc gcagacccgc gcgctcaacg
     2581 gaaaggcgaa cggcgccata ctgggagccg cggaggcagt cgagttcaca ggtaaggcaa
     2641 agacgtcctt tgagctgctg acaccggcgg ggctcgatgt ccgccgcctg gtggtcatcg
     2701 gcgcagggca accggataag gcgaaagaga cggactgggt caatctcggc ggctacgcct
     2761 acgctcagat cacggctcgg aagactgagg ttgccagcct cgttgccgag gttgcggatc
     2821 ccagaacccg atcggctgcc gatatcgcag cggacatggc gttcggtgcg gttctgagga
     2881 gctatcgctt ccagaagtac atcacccgtc agagggagga tgaaaacggg gagcgcaagc
     2941 ccgacgggtt aacgcgcctc atcgtccaca cccaggaccc tgacgcggcg gcaactgcct
     3001 ttgcccgtcg caaggctgtc gccagcggca tctttcttgc gcgcgatctc gtcaacgagc
     3061 ccgcgaacgt tctggggccg gtcgagttcg ccgagcgggt gcaggagctg tccagagttg
     3121 gcgtcgaggt cgaaatcctg gacgccgatg cgctcaaaga gctgaaaatg aatgctcttc
     3181 tcgcggtctc gcaggggagc gttcggccac cgcgcgtcgc tgttatgcag tggaacggcg
     3241 ccaaatcgcg ccgcagcaag ccgctgtgct tcgtcggcaa gggcgtgtgc ttcgataccg
     3301 gcggactatc gatgaagcct gcccagggca tggaggacat gaagggcgac atgggcggcg
     3361 cggcctgcgt tgcaggcctc atgaaaacgc tggcggaacg ccaggcaaac gtcaatgtgg
     3421 tcggccttgt cggactgacg gagaacatgc cctccgggac ggcgcaacgg cccggcgaca
     3481 tcatcacatc catgtccggc cagacgatcg aggtgctcaa caccgacgcc gagggccggc
     3541 tcgtactcgc agatgtgctc tggtatgcgc agcagcgctt caagcctcgc ttcgtcatcg
     3601 atctggcaac gctgaccggc gccatactga tcgcgctcgg gaaagagcac gcggg
//
LOCUS       DASUMG010000211         3105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_830317, whole genome shotgun sequence.
ACCESSION   DASUMG010000211 DASUMG010000000
VERSION     DASUMG010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830317"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFX46_03500"
     CDS             <1..612
                     /locus_tag="VFX46_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5599680.1"
                     /translation="EYIDVALLDSTVASLINQASTYLVSGIVPQRMGNAHPTVVPYQA
                     FRTADGHIILAIGNDGQFQRFCAMAGLDGLADDERYRTNSARILNRDTLIPKIAEAIR
                     QRTTAEWVSLLESQAVPCGPINTIDQVFADPQVQARGMRRVLEHEKAGPLPVTANPVR
                     MASHDTTADKAPPMLGENTDEVLTRLLDLSPDDIAALRNEGVV"
     gene            complement(667..990)
                     /locus_tag="VFX46_03505"
     CDS             complement(667..990)
                     /locus_tag="VFX46_03505"
                     /inference="COORDINATES: protein motif:HMM:NF009316.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HEX5599681.1"
                     /translation="MLAEIVTSLLVLIGGAFALLGSIGLARLPDFFSRLHGPTKATTL
                     GVGSVVMASLVDTAAVEPGLGLRELAITLFLFITAPVSAHMLSKAALSLQHRQTEARQ
                     RDLEP"
     gene            complement(1002..1271)
                     /locus_tag="VFX46_03510"
     CDS             complement(1002..1271)
                     /locus_tag="VFX46_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HEX5599682.1"
                     /translation="MLTYSVALAIAMVIAAMFLNFFRMIRGPSLPDRVVALDTLSINT
                     IALVMLFGISIDSSDYFVVALLIAMMGFVTTAAFCKFMLSGNVID"
     gene            complement(1265..1756)
                     /locus_tag="VFX46_03515"
     CDS             complement(1265..1756)
                     /locus_tag="VFX46_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HEX5599683.1"
                     /translation="MIARVLPRPYLSFIILALWLILAPNFNAGNVFLGGILAIATPLL
                     TASFWPEPLRVQRPLTGLRLLARLIVDILIANIIVARLVVGPIERLQPHFVEVPIDID
                     DDFVATMLGSIIALTPGTVFIDLDRERRVLFIHALNVADPAALVTTIKTRYEAPLKET
                     FGC"
     gene            complement(1753..>3105)
                     /locus_tag="VFX46_03520"
     CDS             complement(1753..>3105)
                     /locus_tag="VFX46_03520"
                     /inference="COORDINATES: protein motif:HMM:NF009309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HEX5599684.1"
                     /translation="AIGEWPAPFGIVLVLDRLSAAMVLLTALLALPALLYAMGGADAK
                     GQLFHPLFQLQLVGINGAFLTGDIFNLFVFFEVLLLASYGLLAHGGGFLRARASLVYV
                     VLNVVGSMLFLAALALIYGMLGTLNFADLALVLPQVPESDQALVRTAFALLVIVFALK
                     AALIPLSFWLPLAYGAAMAPVAALFAILTKVGVYALLRTSTIVLANAGFTANLFEPWL
                     VQVAIVTIALGTAGVLAALRLSVVVAYLVMISTGLMLAVIAVPGVASAAAIIYYMLHS
                     TLVTGGFFLLADAIANERGEHADKLERGPQIRDPTILGSAYLIVAVAVSGVPPLSGFL
                     GKVMLLQSVSGSPWRLPVWIAVLASSLVVALALARTASVLFWEPITDEPRPAYPPTSV
                     LRNLGLMALVAASPLLTLTSGSVSAYARAAAEQLVDRQAYIEAVLGAAPRIIREQRPR
                     "
BASE COUNT          709 a          957 c          956 g          483 t
ORIGIN      
        1 gaatacatcg acgtcgccct gctcgatagc accgtcgcat ccctgatcaa tcaggcttca
       61 acgtatctcg tctcgggcat tgttccgcag cggatgggca atgcgcatcc gacggtcgtg
      121 ccgtatcagg ctttccgcac ggctgacggg cacatcattc tggccatcgg caatgacggc
      181 cagttccagc ggttctgtgc aatggctggt cttgacggac ttgcggacga cgaacgctac
      241 cgcaccaact ccgcccgcat cctcaaccgc gatacactca tcccgaaaat tgccgaggcc
      301 attcgccaac gtacgacagc ggaatgggtc agtctgctcg agtcacaggc ggtgccttgc
      361 ggacccatca acaccatcga tcaggtgttc gccgatcctc aggttcaggc ccgtggcatg
      421 cggcgcgttc tggagcacga aaaggccgga ccgctgcctg tcaccgctaa tccggtccga
      481 atggcctccc acgacacaac cgccgacaaa gcgccaccga tgttgggaga aaacaccgat
      541 gaggtgctga cgcgcctgct cgacctttcg ccagatgaca ttgcggccct tcgcaacgag
      601 ggggtcgttt gatttcactg agtgcgtgct gtttctcggg cgaacgcgat tgtccgccgg
      661 aatagatcac ggctccaaat cccgttgccg agcctcagtt tgacggtgct gaaggctaag
      721 cgcggccttc gaaagcatat gggcgctcac tggggccgtg atgaacagga acagcgtaat
      781 cgccagctcc cgcaacccca agccgggctc gacggcggca gtgtcgacca gcgaggccat
      841 gacgacggag ccgacgccaa gagtcgtggc cttggtcgga ccatgaagcc gggagaagaa
      901 gtccggcagg cgcgcgaggc cgatcgagcc gaggagcgcg aatgcgccgc caatgaggac
      961 caacagcgaa gtgacgattt ctgccagcat ggcgcgtgtc ctcagtcgat cacattgccg
     1021 ctcagcatga acttgcaaaa ggcggccgtt gtcacgaagc ccatcatcgc aatcagcaga
     1081 gccactacga agtagtcgga gctgtcgatg ctgatgccga acagcatcac aagcgcaatg
     1141 gtgttgatcg acagcgtatc gagagccacc acacgatcag gcaggctcgg gccgcggatc
     1201 atccgaaaga aattaaggaa catggctgca atgaccatgg cgatggctag cgcaacggaa
     1261 taggtcagca tccgaaggtc tccttgagcg gggcctcgta acgggtcttg atggtggtga
     1321 cgagcgccgc gggatcggcc acgttcaagg cgtgaatgaa gagaacgcgg cgttctcgat
     1381 cgaggtcaat gaacaccgtc cccggcgtca gcgcgatgat gctgcccagc atcgtcgcca
     1441 cgaagtcatc gtcgatatcg atcggcactt cgacgaaatg cggttgcagt cgctcgatcg
     1501 gccccacgac gagccgcgcg acgatgatgt tggcaatcag aatatcgaca atgagccgag
     1561 ccaaaaggcg caggcctgtg agcggccgtt gcactctaag aggctctggc cagaacgacg
     1621 ccgtcagaag cggtgtggcg atcgccagaa ttccgccgag gaagacattt cccgcgttga
     1681 aattcggcgc cagaatgagc cacagcgcca aaataatgaa gctgagatac ggcctcggca
     1741 gaacacgggc gatcatcttg gccgctgctc ccgaatgatg cgtggtgccg cgccgagcac
     1801 agcctcgatg taggcctgcc ggtcgacgag ctgttctgcc gccgcccgtg cgtaggcaga
     1861 gacggagccg gaggtcagcg tcagcagcgg gctagccgcg acaagtgcca tcagccccag
     1921 atttcggagc acgcttgtcg gcggataagc tggccgcggc tcgtcggtga tcggttccca
     1981 gaaaaggacg ctcgcggtcc gcgccagcgc cagcgcaacg acaaggcttg atgcgagtac
     2041 cgcaatccac accggaagcc tccacgggct tccgctgacc gactgcaaca gcatcacctt
     2101 gcccaagaac ccggaaagcg gcggcacacc gctgaccgcg accgcaacga tgagataggc
     2161 cgagccgaga atggtggggt cgcgtatctg cggaccgcgc tccagcttgt ccgcatgctc
     2221 gccgcgctcg ttcgcgatcg cgtccgccag cagaaagaat ccgccggtaa ccagcgtgga
     2281 gtgcagcatg tagtagatta tggctgctgc gctcgcgacc cccggcacgg cgataaccgc
     2341 cagcatcaag ccggtcgaaa tcatcaccag ataggcaacg accacggaca ggcggagcgc
     2401 tgccagcact ccggctgttc caagcgcgat cgtcacaatg gcgacctgca ccagccaggg
     2461 ctcgaacagg ttcgctgtga agcccgcatt ggcgagcacg atggtggagg ttcggaggag
     2521 agcatacact ccgacctttg tgaggatcgc gaacagcgct gccactggag ccatagcggc
     2581 accgtaggca agcggcagcc agaacgagag cggaatcagg gccgccttga gcgcgaagac
     2641 gatgaccaac agcgcgaatg ccgtccgcac caacgcctga tccgattccg gaacctgcgg
     2701 cagcacaagc gcgagatcgg cgaagttgag ggtgcccagc atgccataga tcaacgcgag
     2761 ggccgcgaga aacagcatcg agccgacaac attgagcacg acgtagacga ggcttgcccg
     2821 cgcgcgtaga aacccgccgc catgggccag caggccatag gaggcgagca gaagcacctc
     2881 gaaaaagacg aacagattga agatatcgcc cgtcaggaaa gcaccgttga tcccgacaag
     2941 ctgaagctgg aacagcggat gaaacagctg acctttcgca tccgcccctc ccatggcgta
     3001 gagcaaggcc ggaagcgcaa gcagcgccgt cagcaggacc attgcggctg agagacgatc
     3061 cagcaccagc acgatcccga agggtgctgg ccactcgccg atggc
//
LOCUS       DASUMG010000212         3297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_911256, whole genome shotgun sequence.
ACCESSION   DASUMG010000212 DASUMG010000000
VERSION     DASUMG010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911256"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..145)
                     /locus_tag="VFX46_03525"
     CDS             complement(<1..145)
                     /locus_tag="VFX46_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599685.1"
                     /translation="MSRERLYLFDTTLRDGAQTSGVNFSLEDKRRIAELLDGLGIDYI
                     EGGY"
     gene            complement(283..681)
                     /locus_tag="VFX46_03530"
     CDS             complement(283..681)
                     /locus_tag="VFX46_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5599686.1"
                     /translation="MDNANNAPTLDHIGLQVSDFARAKEFYSAALQPLGIKLQMELPN
                     EAGGVAGFGRCKPEFWIAAGKTPTSPFHIAFHAANRAQVDAFYKAAIAAGARDNGAPG
                     LRPQYHPNYYGAFVIDPDGHNVEAVCHYPE"
     gene            complement(697..2088)
                     /gene="cysS"
                     /locus_tag="VFX46_03535"
     CDS             complement(697..2088)
                     /gene="cysS"
                     /locus_tag="VFX46_03535"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX5599687.1"
                     /translation="MTLTLYNTLTRRKEPFRPLDPKNVRMYVCGPTVYDYAHIGNARP
                     VIVFDVLFRLLRHIYGPEHVTYARNITDVDDKINARAAEEGISIRELTERTTRQFHED
                     IAALGVLPPTVEPRATDHIEDMKRMIERLVEKGCAYVAQDHVLFDVSKMPDYGRLSNR
                     SLEEMEAGARVEVAPYKKGPMDFVLWKPSKPGEPSWPSPCGIPTPGRPGWHIECSAMA
                     ARHLGEVFDIHGGGIDLVFPHHENEIAQSRCAHGTPIMAQVWMHNGFLQVEGEKMSKS
                     LGNFVTIHELLATDKFGGRSWPGEVLRFNMLRTHYRQPIDWTVEGLEQSLTILDSWFP
                     FASEYTDTDRQKAGPVIEALCDDLNTPKAIAELHRLHDAGERGALGFALALLGFTGET
                     IKRERERVERKRQELAAIVEPRISARLAARKAKNFAEADRIRDELAAMGIQLKDSKDS
                     ATGEIVTTWEIKR"
     gene            complement(2272..2811)
                     /locus_tag="VFX46_03540"
     CDS             complement(2272..2811)
                     /locus_tag="VFX46_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX5599688.1"
                     /translation="MLFDSWSGVLRVLVVGTLAYAGLVVLLRISGKRTVARMSAFDLA
                     VTVALGSTLAMVVLLKTIPLAEGLLAIALLILLQFVVSWLSSRSPGFRRIIKARPSLL
                     LSDGKFIEEALLEERVAREEVMAAVRASGYESLDQVAAVVLEADGTLSVIGHPTPGTE
                     SAMRNVEGREATRHLEHPA"
     gene            3025..>3297
                     /locus_tag="VFX46_03545"
     CDS             3025..>3297
                     /locus_tag="VFX46_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599689.1"
                     /translation="MRLIKRGTSNRRYGGLATAVALLLLATGPVLAQGWPWEWGWGRE
                     PPTPREPVFREPPQQQPLGYQGYDSSRSSICLQLEQRLAQEANRGAQ"
BASE COUNT          612 a         1007 c         1028 g          650 t
ORIGIN      
        1 gatacccacc ttcgatatag tctatgccaa gcccatccag cagctccgca atgcggcgct
       61 tgtcttcgag agagaaattc acccccgatg tttgcgcgcc gtcgcgcagc gtcgtgtcga
      121 aaagataaag gcgttcgcga gacaatgttc tgtcctcaga tgatatcgac aagacggagg
      181 gcaaggagga ccgccgcgat gatcacggcg atcttgagaa tgaggtagta gatgcggtgg
      241 cgctcaaacg gtggggtcgc gtcgggctcg gtcattgttt tctcactcag gataatggca
      301 gaccgcctcg acgttgtgcc catcgggatc gataacgaag gcgccatagt aattggggtg
      361 gtactgcggg cgtaaccccg gcgctccgtt atctcttgcg ccagccgcga tggcagcttt
      421 gtaaaacgca tcgacttgag ccctgttggc cgcgtggaaa gcgatgtgga aagggctcgt
      481 cggcgtttta ccggcggcaa tccagaattc cggcttgcag cggccaaatc ccgccacgcc
      541 gcccgcttcg ttcgggagtt ccatctgcag ctttatcccg agcggctgca atgcggcgga
      601 atagaattcc ttggcgcggg cgaaatcgct gacctgcaga ccgatatgat caagtgtggg
      661 cgcgttgttt gcattgtcca tagcgtatct ccggctctag cgcttgatct cccacgtcgt
      721 aacgatctcg ccggtcgcag agtccttgga gtccttgagc tggatcccca tggccgcgag
      781 ctcgtcgcga atgcggtctg cttcagcgaa atttttggct ttgcgtgccg ctaatcgagc
      841 gctgatgcga ggttcgacga tggccgcaag ctcctgacgc tttctttcga cccgctcccg
      901 ctcgcgcttt atcgtctcgc cagtaaaacc aagtagtgcc aaggcgaagc ccaaagcgcc
      961 gcgttctcca gcatcatgca atcggtgcag ctccgcgatg gccttgggcg tattcaagtc
     1021 atcgcacaaa gcctcaataa ctggcccggc tttttgtcga tccgtgtcgg tgtattcgct
     1081 cgcgaatgga aaccacgaat cgagaatagt gaggctctgc tccaagcctt caaccgtcca
     1141 gtcgatcggc tgccggtaat gcgtccggag catattgaag cgtagaacct ctcccggcca
     1201 ggaacgcccg ccgaatttgt ccgtcgccag caattcgtgg atcgtcacga aattccccag
     1261 gctcttggac attttctcgc cctcgacctg gagaaagccg ttgtgcatcc acacctgcgc
     1321 catgatcggc gtgccgtgcg cgcagcggga ttgtgcgatt tcgttctcgt gatgggggaa
     1381 gacgagatcg atgccgccgc cgtgaatgtc gaaaacctcg cccaagtgcc gcgcggccat
     1441 ggccgagcac tcgatatgcc aacccggccg gccgggcgtg ggaatgccac aaggactcgg
     1501 ccaagaaggc tcgccgggct tggacggctt ccagagaacg aagtccatcg gccccttttt
     1561 gtatggcgcg acctcgacgc gcgcgccggc ctccatctcc tcgaggctgc gattggagag
     1621 gcggccatag tcgggcatct tgctgacgtc gaacagcacg tggtcctggg cgacgtaagc
     1681 gcagcccttt tcgaccaggc gctcgatcat ccgcttcata tcctcgatgt gatcggtggc
     1741 gcgcggctcg actgtcggcg gcagcacgcc aagggcggcg atgtcttcat ggaactgccg
     1801 cgttgttcgc tcggtcagct cgcggatcga gatgccttcc tcggccgccc gcgcattgat
     1861 cttgtcatcg acgtccgtga tgttgcgggc gtaggtgacg tgctcgggcc cgtagatgtg
     1921 ccggaggagc cggaacagca catcgaaaac gatgacaggc cgcgcgtttc caatgtgcgc
     1981 atagtcgtag acggtcgggc cgcacacgta catacgcacg ttcttgggat cgagcggccg
     2041 gaacggctcc ttgcggcggg tgagggtatt atagagcgtg agcgtcactg tgttcccctg
     2101 acctgctggc ggggcttggc tcttttacgg gagaataggg gagcggccgc gcgccagcga
     2161 taagctagct cggaataatg atgcaaataa tgccgctgta gatgcgcgcg gcggacatgg
     2221 tcgcggctct gtccatcatg cggtgcttat gcgtgaggcc ggtgggggac gtcaagcggg
     2281 gtgctcgaga tgccgcgtgg cctctcgccc ttccacgttg cgcatcgccg attcggtgcc
     2341 gggcgttggg tgtccgatca cgctgagggt tccgtcagcc tccagcacta ccgccgccac
     2401 ctgatccaag ctttcgtatc cgctggcccg cacggcggcc atcacctcct cccgtgcgac
     2461 gcgctcttcc agcagggcct cctcgatgaa cttgccgtcg gacaacagca ggctgggccg
     2521 cgccttgatg atccggcgga agcccggcga gcgggatgag agccaggaaa ccacgaactg
     2581 aagcagaatg aggagcgcga tcgccagcaa accttccgcg agcgggatcg tcttcagcag
     2641 cacgaccatc gccagggtcg agccgagcgc cacggtcacc gcgaggtcga aggcgctcat
     2701 cctcgccacc gtgcgcttgc cactgatgcg cagcagcacc acgaggccgg cataggcgag
     2761 agtgccgacc accaacacgc gcagcactcc tgaccagctg tcgaacagca cccgagcacc
     2821 ttttccgagc cgatatgctg cggctcgagt actttcaacg gccgagcgct ccattcgttc
     2881 ggggatgcgt tgcccggcat ctacatcgag ggggtgcccc atttacgctg cattgacccc
     2941 atagcaaaat tgattcaagg aaacgggaga ccaggctggg ccaagccaaa gcggcgtggc
     3001 tcgcccggag gggtgtgatt cgacatgcgc ctcatcaagc gtgggacatc gaatcgccgc
     3061 tatggcggcc tggcgaccgc cgtagcgttg ctcctgttgg caactggtcc cgtgctggcc
     3121 cagggctggc cttgggagtg gggttgggga cgggagcctc caactccgcg cgagcccgtt
     3181 ttccgtgagc ccccgcagca gcagccgcta ggatatcaag gatacgactc aagccggtcc
     3241 tcgatctgtc tgcagctcga gcagcggctt gcccaggagg ccaatcgcgg ggcacag
//
LOCUS       DASUMG010000213         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_510302, whole genome shotgun sequence.
ACCESSION   DASUMG010000213 DASUMG010000000
VERSION     DASUMG010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510302"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFX46_03550"
     CDS             <1..291
                     /locus_tag="VFX46_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX5599690.1"
                     /translation="GPMNNASPFTMSGVAGILELIGGTLLVIGLFSRPVAFILSGMCA
                     VAYFYAHAPRGFYPILNGGELAALYSFVFLYLAAAGGGIWSVDRLWKEKEAA"
     gene            complement(388..1212)
                     /gene="panB"
                     /locus_tag="VFX46_03555"
     CDS             complement(388..1212)
                     /gene="panB"
                     /locus_tag="VFX46_03555"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX5599691.1"
                     /translation="MSNQGSVSRLTSVDIRARKGGAPIVSLTAYGANIAAIADNHVDF
                     LLVGDSVGMVVHGFESTLPVSLEMMIMHGAAVVRGTKRALVVVDLPFGSYEESPEVAF
                     RSAARIMRETGCGAVKLEGGRHMAETIRYLVERGIPVMGHIGLTPQSVNTLGGFRAQG
                     RTEDQWNAIEADAKAVSDAGAFAIVLEAMAEPLAARLTKAVPVPTIGIGASSACDGQI
                     LVMDDMLGITPRAPKFVKQFGQIGQAIDRAIADYAASVRNRSFPAPEHTYGMKPKG"
     gene            1321..2604
                     /gene="otnK"
                     /locus_tag="VFX46_03560"
     CDS             1321..2604
                     /gene="otnK"
                     /locus_tag="VFX46_03560"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HEX5599692.1"
                     /translation="MQESLPVLLGCIADDLTGATDLALMLSRNGMRTVQVMGVPDAST
                     LTDGADAVVVALKSRTNPADEAVRWSLASAEALLARGARQLFFKYCSTFDSTDRGNIG
                     PVAEALLEKVRGEIALVCPAFPTNSRTVYMGHLFVGNVLLHESPMKDHPLTPMRDSNL
                     VRVLQRQTQLKVGLVPYSVVDRGPDAITAALNEAARAGTRFAVADAISDRHLVSLGAA
                     AADHPLITGGSGLAMGLPDNFARQGLLQKAEPARHMPAPPGRALIIAGSCSEATRGQV
                     QSAMAAGIPAFKLDPLAIAQGKLAASDLVSWALKQPVDRPVLIYSSDEPGNVRAVQSE
                     LGREAAGAMVERELAAVAKELVEKGFTRLIVAGGETSGAVVGALGIRAIEIGPEIDPG
                     VPWTRSMDDRGLVLALKSGNFGALDFFMKAWDLIG"
     gene            2601..3239
                     /gene="otnC"
                     /locus_tag="VFX46_03565"
     CDS             2601..3239
                     /gene="otnC"
                     /locus_tag="VFX46_03565"
                     /EC_number="4.1.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate 4-phosphate decarboxylase"
                     /protein_id="HEX5599693.1"
                     /translation="MTTGAKLREEICAAGASLFNRGLTHGSTGNISVRLEDGGWLMTP
                     TGSSLGTLDPARLSKLDPDGRLVSGDPPTKEAFLHTTMYHARPNAGAVVHLHSTYSVA
                     VSCLEGIDPADCLPPITAYYVMRVGALPLVPYYPPGDKDLAEAVGKLADRHHAVLLAN
                     HGPVVAGTSLSAAMDAIEELEETARLYLLLRGQPIRTLTDDQVAELRRRFPS"
BASE COUNT          586 a         1036 c         1083 g          668 t
ORIGIN      
        1 gggccgatga acaatgcttc gcccttcacc atgagcggcg ttgccggcat tcttgagctg
       61 atcggcggaa cgcttctcgt aatcgggctg ttctcgaggc cggtggcgtt cattctttcc
      121 ggcatgtgcg cggtggccta cttctatgcc cacgcgccgc gcggcttcta cccgatattg
      181 aatggtggcg agctggcagc tctgtactcc ttcgtgttcc tctatctcgc ggccgccggc
      241 ggtggcatct ggagcgtcga ccggctttgg aaagaaaaag aagcggcttg agccgggttt
      301 aacgcagctt ccaagccgtc tccgggactt cgaagctgcg ttagctttcg tttcgggcgt
      361 gtaggtcggg agcttgtccc accccgtcta gcccttcggc ttcatcccgt aagtgtgctc
      421 aggcgcaggg aagctccgat tacggacgct ggcggcgtag tcggcgatcg cgcggtcgat
      481 ggcttggccg atctggccga actgtttcac gaacttgggc gcgcgcgggg tgatgccgag
      541 catgtcgtcc atcaccagaa tttgcccgtc gcacgccgag gacgcgccaa tgccgatcgt
      601 ggggacgggc acggctttgg tcagccgcgc cgcgagcggt tcggccatgg cctcaagcac
      661 gatagcgaaa gcacctgcgt cgctcaccgc tttcgcatcg gcttcgatcg cattccattg
      721 gtcctccgta cgtccttggg cacggaagcc gccgagcgtg ttcaccgatt gcggggtgag
      781 gccgatatgg cccatcaccg gtatgccgcg ttcgaccaga tagcggatgg tctcggccat
      841 gtggcgccca ccttcgagct ttaccgcgcc gcatcccgtt tcgcgcatga tgcgcgcagc
      901 actccggaag gcaacttccg ggctctcctc gtaggagccg aacggcaggt cgaccacgac
      961 cagcgcacgc ttggtgccgc ggaccaccgc cgcgccgtgc atgatcatca tctcgagcga
     1021 cactggcagg gtgctttcga acccgtgaac caccatcccg acactgtcgc ccacgagaag
     1081 gaaatcgacg tggttgtcgg caatcgcggc gatgttggca ccgtaagcgg tcagcgagac
     1141 gatcggcgcg ccgcccttgc gagcgcggat gtcaacagag gtcaatcgcg agacggaacc
     1201 ttggttggac acggggcagt ctcctggaag tttgggacga tctatcaacc tcgggctgcg
     1261 gagccaaggc tggtggtgct ctctcgtgcg tgctgaggta taggtctgcg atgggctgac
     1321 atgcaggaga gtcttcccgt gctacttggc tgcatcgccg acgatctgac cggcgccacc
     1381 gaccttgcgc tcatgctgag ccgcaacggc atgcgcaccg ttcaggttat gggcgtaccc
     1441 gatgcatcaa cgctcacgga cggcgcagat gcagtcgtcg tggcgctcaa gtcgcgcacc
     1501 aatccggccg acgaggccgt gcgctggtcc ctggccagcg cggaggcctt gctggcgcgc
     1561 ggggcgcggc agctgttctt caagtactgc tccaccttcg actcgaccga ccgaggcaat
     1621 atcggccccg tggcggaagc gctcctcgaa aaggtgaggg gtgaaattgc gcttgtttgc
     1681 ccggcttttc caaccaattc gcgcaccgtt tacatgggcc atctcttcgt cggcaacgtg
     1741 ctcctgcacg aaagcccgat gaaggatcac ccgctcaccc ccatgcggga ttccaatctg
     1801 gtgcgggtgc tacagcggca gacacaactg aaggtcgggc ttgttcctta tagcgtggtt
     1861 gatcggggcc cggacgccat cactgccgcg ctcaacgagg ctgcccgagc tggaacgcga
     1921 ttcgcggtgg ccgatgccat cagtgatcgg catctcgtgt cgctgggggc cgccgcggcg
     1981 gaccatccac tgattacggg cggctcggga ctcgctatgg ggctgcccga caacttcgcc
     2041 cggcagggcc tgttgcaaaa agccgaaccc gcaaggcata tgccggcgcc gccaggccga
     2101 gccctcatta ttgctggtag ctgctctgag gcgacacgcg ggcaggtgca gtcagcgatg
     2161 gcggcaggaa tccctgcgtt caagctcgat ccgcttgcga ttgcacaagg aaagctcgcc
     2221 gcgtcggacc tggtgagctg ggcactgaag cagcccgttg atcggccagt tctgatctat
     2281 tccagcgacg agccaggcaa cgtgcgggcc gtgcagtccg aactcggccg cgaggctgcg
     2341 ggtgccatgg tcgagcggga gcttgcggcc gtagcaaaag agcttgtcga gaaggggttc
     2401 acgcgcctca tcgtggcggg tggcgagacc tcaggtgcgg tcgtcggcgc cctgggtatc
     2461 agggccatcg agatcggccc ggaaattgat cccggcgttc cgtggacgcg cagcatggat
     2521 gaccgcgggt tggtgctggc gttgaagtcg ggcaatttcg gcgcgttaga tttcttcatg
     2581 aaggcgtggg atctcatcgg atgacgacag gggcgaagct tcgcgaagag atttgcgctg
     2641 ctggcgcctc gctcttcaat cgcgggctga cgcatggctc gacgggaaat atttccgttc
     2701 ggctcgaaga cggcggctgg ctgatgacgc cgactggctc gtctcttggc acactcgacc
     2761 cggcacgctt atcgaaactc gatccagacg gacggctcgt gtctggcgat ccgccgacga
     2821 aggaggcctt cctccacacg accatgtacc atgcgcggcc taatgccggg gctgtcgtgc
     2881 atctgcattc gacctactcg gtggccgtca gttgtctcga aggcatcgat cctgcggatt
     2941 gcctgcctcc gatcacagcc tattacgtga tgcgcgtcgg cgcgctgccc cttgttcctt
     3001 attatcctcc gggcgacaaa gatttggccg aagcggtcgg caagctcgct gaccgccatc
     3061 acgccgtgct tcttgcgaac cacggccctg tggttgcggg cactagcttg tcggccgcaa
     3121 tggatgcgat cgaagagctg gaggaaacag cgcgcctata tctccttctt cgcgggcaac
     3181 caatccgaac cctgacggat gaccaagtcg ccgaattgcg ccgtcggttt ccaagctagg
     3241 tgctccattg cgcagatccg cctaagttcg accccgttgc gctgtgcatc gcgttggcgg
     3301 cgcgccctta aagcagtgcc caggtgaaga tcgaggaatt caaacaagga gaggaaacgg
     3361 aaatggcgaa taa
//
LOCUS       DASUMG010000214         4906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_28070, whole genome shotgun sequence.
ACCESSION   DASUMG010000214 DASUMG010000000
VERSION     DASUMG010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28070"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFX46_03570"
     CDS             complement(<1..414)
                     /locus_tag="VFX46_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5599694.1"
                     /translation="MTRTLVIIPARMQATRLPGKPMADIHGKPMIVHVWERARAADVG
                     RVVVATDTEEIASAIRNVGGEAVLTRPDHASGSDRVHEALQRIDAGGAVDFVINLQGD
                     LPTLEPHLVRECLAPLMSPGPDIATLAAEIREPEEK"
     gene            592..1143
                     /locus_tag="VFX46_03575"
     CDS             592..1143
                     /locus_tag="VFX46_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX5599695.1"
                     /translation="MDTREFNKIAGAVLTALLLIFGTATFIDIQMRHKKPAKPGYELP
                     VQTTTAAETSQPAAAAGVNFQEIAGLLQNASPEAGQAAFKRCSTCHTPEKGGRNMTGP
                     NLWGIVGRPKGSQEGFNYSAAMKEKGGEWTYEDLANFIHNPRAFIPGNKMAFAGIRDN
                     AEIADLLAYLRTLSDNPPPLPSN"
     gene            1365..3245
                     /locus_tag="VFX46_03580"
     CDS             1365..3245
                     /locus_tag="VFX46_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5599696.1"
                     /translation="MVTHVKGSIGKSFLALAAAVLALGATMEVPQAGEEQKRHHALSL
                     VGEPAYGPDFKHFDWVNPDAPKGGTVRQWAMGSFDSLNPFSIQGSAAAGLGLIYDSLM
                     ASSPDEPSTEYGLIAEWVSFPDDFSSATFGLRPEARFHDGEPIKPEDVIFSLEALKKS
                     HPHYNAYYRNVVRAEKTGDHEVTFIFDQKGNRELPQIVGQLPVLPKHFWEAKGANGQP
                     RDLTRSTLEIPLGSGPYKIKSVDPGRSIVYERVKDWWAKDLPVTKGQWNFDELVFTYY
                     RDRVPAFEAFKIGEIDYWQETSAKAWATSYDFEAVRRGLVKKEEIPIRRVTPMQAFVM
                     NLRRPQFQDIRVRKAFNLAFDFEWMNKNLFFDQYKRTESFFGNSELQATGLPTGRELE
                     ILNEIKDEVPPEVFTTEWKNPVNATPQDQRNHLRQAAQLLAEAGWKPKNGVLTNDKGE
                     QLAVEFLLVQPDFERVVLPFKTNLERLGVRVTIRIVDTSQYQRRVDTFDYDIIVDSFP
                     QSNSPGNEQRNFWGSAAADMHGSQNSIGIKNPAIDKLIDKIIYAPNREELVAATRALD
                     RVLLWNYYVIPQWHSPYDRIAVWDMFGRPDKLPSQSPSFLQVWWLDAEKQKELNAKRG
                     RG"
     gene            3242..>4906
                     /locus_tag="VFX46_03585"
     CDS             3242..>4906
                     /locus_tag="VFX46_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5599697.1"
                     /translation="MRHDRKALFGHGSGQIIRVATSVLFGLLAAASGVAADEARHGLS
                     AFGELKYGPDFQHFDYVNPNAPKGGRLAHIGPAATITFDSFNPFILRGDAAQGLELLF
                     DSLMVRAMDEPDAVYGLVAKTAKLAPDRRSVTFQMRPEAKFSDGSPLTAEDVVFTFET
                     LKTKGHPHYRILLRDVVRAEALGPHEVRFVFQGENLRDLPLTVATLPILSKAYYATRE
                     FDQTTLEPPLGSGPYRISDFKQGSYTTFKRREDYWAKDLPVNRGRYNFDEIRYEYFRD
                     RTAALESLKAGAYDLREEFTAKDWATSYDIPQVKDGRIVLLTLPDESPSGAQGFFLNN
                     RRAKFSDRRVRKALDYAFDFEWTNANLFYSLYTRTASYFENSEMKATGKPSPEELALL
                     EPYRDQLPPEVFDEPYVPPVSDGSGSDRRLLRTAAKLLEEAGWELKGNQRVNKNGEVL
                     SIEFLVYEPTFERVIAPYIKNLKSLGIDAYIRRVDPAQFERRVKAFDFDIITQRFSMR
                     LTPGAELLNFFGSEAADTEGSFNRAGIKSPVVDALAMKALEAQSREE"
BASE COUNT          986 a         1557 c         1488 g          875 t
ORIGIN      
        1 tttttcttcg ggctccctga tctccgcggc gagggtggcg atatccgggc cgggactcat
       61 cagaggcgcc aagcattcac gcaccaggtg cggctcgagc gtcggcaaat ccccctgcag
      121 attgatcacg aaatccacag cgccgcctgc gtcgattcgc tgcaaagctt cgtgcacccg
      181 gtccgagccg gacgcatgat ccggacgggt caagacggct tcgccgccga cgttgcggat
      241 ggcggatgcg atctcctccg tatctgtcgc aacgacgacc cggccgacgt cggccgcacg
      301 cgcacgctcc cagacatgca caatcatcgg cttgccgtgg atgtccgcca tcggcttgcc
      361 gggaagacgc gtggcttgca tacgggccgg aatgatgacg agcgtgcggg tcatgggcgt
      421 gcagcttcga gcaggactga cgttttcggc gagtgacaat tggtctcggc acgagcgact
      481 ggaccgaacc gccaccccgg ttgcatgaag attgggactt tctatagtcg gccgccgatc
      541 ggggcacgag tcataagaag cgactggcgc gggcagaggg ctggggattc gatggatacc
      601 agggagttca acaaaatcgc tggggccgta cttacagcgc tcctcctgat tttcggaacg
      661 gcgacgttca tcgacatcca aatgaggcac aagaagcccg ccaagccggg ttacgagctg
      721 ccggtccaga ccaccactgc agcggagacg tcccaacctg ccgcagctgc tggcgtgaac
      781 ttccaggaaa ttgccggttt gctccagaac gcgagccccg aggccggaca ggcggcgttc
      841 aagcgctgct cgacgtgcca cacgcctgag aagggtgggc gcaacatgac cgggcccaac
      901 ctgtggggca ttgtcggcag acccaagggt tcgcaggaag ggttcaacta ctcggcggcc
      961 atgaaggaaa agggcggcga gtggacttac gaggatctgg ccaacttcat tcacaacccg
     1021 cgcgctttca tccctggcaa caagatggcg ttcgccggca tcagggataa tgccgaaatc
     1081 gccgacctgc tcgcttacct gcggacattg agcgacaatc cgccgccgct tccaagcaac
     1141 tgagcagtct gcaggagccc cctatatcga gggtgctcgc ggagccttgt tggctagatt
     1201 gtgatcgagc cacaaccgtg tgcgacctgg cctatagatt ggcgaggtcg cttttgctgt
     1261 tataacggca tcggcagccg ctccgcagcc acggaggcgg caattgtggc ccatgtcccg
     1321 aggagccatg gaccactatt ccaccactca ggcgggagtt ttgtgtggtc acacacgtca
     1381 agggtagcat aggcaagagt ttcttggcgc tggcggcagc tgtgctggcc ctcggcgcga
     1441 caatggaggt cccccaggct ggcgaagagc agaagcggca tcacgcgctt tcgctcgtcg
     1501 gcgagccggc atacggaccg gatttcaagc acttcgattg ggtcaatccg gacgcgccca
     1561 aaggcggcac agtgcgccag tgggctatgg gctcgttcga cagtctcaac cccttctcta
     1621 tccaaggcag cgcggcggcg ggcctcggcc tcatctacga ctctctgatg gcttcgagcc
     1681 cggacgagcc ctcgaccgag tacggcctga tcgccgaatg ggtttccttc ccggatgact
     1741 tttccagcgc caccttcggc ttgcgccctg aggctcggtt ccacgatggt gagccgatca
     1801 agccggaaga cgtcatcttc tcgctcgagg cgctcaagaa gtcgcacccg cactacaacg
     1861 cctattaccg caacgtcgtc cgggccgaga aaaccggcga ccatgaggtg accttcatct
     1921 tcgaccagaa gggcaatcgc gagctgccgc agatcgtcgg ccagttgccc gtgttgccga
     1981 agcacttctg ggaggcgaag ggcgccaacg gccagccgcg cgacctcacc cgaagcacac
     2041 tggaaatccc gttgggctcc ggaccgtaca agatcaagtc ggtcgatccg ggccgcagca
     2101 tcgtctacga gcgggtgaaa gactggtggg cgaaggacct gcccgtcaca aagggccagt
     2161 ggaacttcga cgagctcgtc ttcacctact atcgggaccg cgtgcccgca ttcgaggcct
     2221 tcaagatcgg cgagatcgat tactggcaag agacgagcgc caaggcctgg gccacctcct
     2281 acgacttcga agcggtccgc cgcgggcttg tgaaaaagga agagatcccg atccgccgcg
     2341 tgacgccgat gcaggcattc gtcatgaatc tgcgccggcc gcagttccag gacatccgcg
     2401 tgcgtaaggc cttcaatctg gctttcgatt tcgagtggat gaacaagaac ctgttcttcg
     2461 accagtacaa gcggacagag agcttcttcg gcaattcgga gctgcaggcg acagggctgc
     2521 caacaggccg cgaactcgaa atcctgaacg agatcaagga tgaagttccg ccggaggtct
     2581 tcacgaccga atggaagaat ccggtgaacg ccacgccgca ggaccagcgc aaccatctgc
     2641 gtcaggcggc gcaacttctc gcggaggccg gctggaagcc gaagaacggc gtgctgacca
     2701 acgacaaagg cgagcagctg gccgtcgagt tcctgctcgt tcagccggat ttcgagcgcg
     2761 tcgtcctgcc gttcaagacg aaccttgagc gcctcggcgt tcgcgtgacc atccgcatcg
     2821 tcgacacctc gcaataccag cgccgtgtcg acaccttcga ttacgacatc atcgtcgact
     2881 cattcccgca atcgaactcg ccgggcaacg agcaacgcaa tttctggggc tctgccgctg
     2941 ccgacatgca cggcagccag aactccatcg gcatcaagaa cccggccatc gacaagctga
     3001 tcgacaaaat catctacgcg ccaaaccgag aggagctggt ggcggccacc cgggcgctcg
     3061 atcgggtgct gctgtggaac tactacgtca tcccgcaatg gcactcgccc tacgaccgca
     3121 tcgccgtatg ggacatgttc gggcggcccg ataagctgcc aagccaaagc ccgtcgttcc
     3181 tgcaggtctg gtggcttgat gccgagaagc aaaaagagct caacgcgaag cgggggcggg
     3241 gatgagacac gaccgcaaag ccctctttgg tcacggctct ggccagatca tccgtgtcgc
     3301 gacgagtgtt cttttcggcc ttcttgctgc tgcttcgggc gtcgcagcgg atgaagcgcg
     3361 ccatggatta tccgcgttcg gcgagctgaa gtacggcccg gactttcagc actttgacta
     3421 cgtcaacccg aacgcgccaa agggcggtcg cctggcgcac atcggtccgg ccgcaaccat
     3481 cactttcgac agcttcaacc cattcatcct gcgcggggat gcagcccagg gactcgagct
     3541 tctcttcgac agtctcatgg tccgcgccat ggacgagcct gatgccgttt acggcctcgt
     3601 cgcgaaaacg gcgaagctcg cacctgatcg ccgttccgtg acgttccaga tgcgccccga
     3661 ggccaagttc tcggatggct cgccgctgac ggcggaggac gtcgtcttca ccttcgagac
     3721 cctgaagacg aagggtcatc cgcattaccg catattgctt cgtgacgtcg tccgggccga
     3781 ggcccttggg ccccatgagg tgcgcttcgt ctttcagggc gaaaacctgc gcgacctgcc
     3841 gctgaccgtc gccacactgc cgatcctgtc gaaggcctac tacgcgacgc gcgagttcga
     3901 tcagaccacg ctcgaaccgc cgctcgggtc cggcccgtac cgaatttccg acttcaaaca
     3961 aggcagctac accaccttca agcgccgcga ggattattgg gccaaggatc tgcccgtgaa
     4021 ccgcgggcgc tataacttcg atgaaattcg ctacgaatat ttccgcgacc gcacggcagc
     4081 gctcgaaagc ctgaaggctg gcgcatacga ccttcgcgag gagttcaccg ccaaggattg
     4141 ggcgacgagc tacgacatcc cgcaggtcaa ggatgggcgc atcgtcctgc tgaccctacc
     4201 tgacgaaagc ccctccgggg cgcagggctt cttcctcaac aatcggaggg ccaagttctc
     4261 cgaccggcgc gtgcgaaagg cgctcgacta cgccttcgat ttcgagtgga cgaacgccaa
     4321 cctcttctac tcgctgtaca cgcgcacggc gagctacttc gaaaactccg agatgaaggc
     4381 caccggcaag ccttcgccgg aagagctggc gctgctcgag ccttatcgcg accagctgcc
     4441 gcccgaggtc ttcgatgagc catacgtgcc gcctgtctcc gacggctccg gctcggatcg
     4501 ccgcctgctg cggacagcgg ccaagcttct cgaagaggcc ggctgggagc tcaagggcaa
     4561 ccagcgggtc aacaagaatg gcgaggtgct ctcgatcgag ttcctggttt acgagccaac
     4621 gtttgagcgt gtcatcgcgc cttacatcaa gaacctgaag tccctcggca tcgacgccta
     4681 tattcgccgc gtggacccgg cgcagttcga gcggcgcgtg aaggcgttcg atttcgacat
     4741 catcacccag cggttctcca tgcggctgac gccgggagcg gagcttctca acttcttcgg
     4801 cagcgaggcc gccgatacgg agggcagctt caaccgcgcg ggtatcaaga gccccgtcgt
     4861 ggacgccctg gcgatgaagg cgttggaggc ccagtcgcgg gaggag
//
LOCUS       DASUMG010000215         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_349698, whole genome shotgun sequence.
ACCESSION   DASUMG010000215 DASUMG010000000
VERSION     DASUMG010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349698"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..>2239
                     /locus_tag="VFX46_03590"
     CDS             540..>2239
                     /locus_tag="VFX46_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599698.1"
                     /translation="MTAEVIADSAPRVDAAEAAASSPWLLEVENLHVHFVTTAGVVRA
                     VEGLSYKVKPGEVVALVGESGCGKSVSALTVMRLLAKPAGRVVAGRILFQGRDLLKLS
                     EEEMRAIRGREISMIFQEPMTSLNPVLTIGFQIMEPLMIHLGMSEKQARARAAELMRM
                     VGIPDGERRLDQYPHQFSGGMRQRVMIAIGLACNPKLIIADEPTTALDVTIQAQILQL
                     MKDLSRELGIAMLIITHNLGVVARYADRVNVMYAARMAEQGTADDVFARPRHPYTYGL
                     LRSVPRLDRPREAKLETIEGLPPNLVSPPPGCRFAPRCAAATEACKVAPPPLVEVESG
                     HYSACIRATELAEKGPQAIGLSKPAGPAKLEVPRSESPPLLRLERLTKHFVVSSGSRL
                     FGRSSAIVRAVNDVSFSIAPGQTLGLVGESGCGKTTVGRLVLKLEEPTSGSIFFEGKD
                     LTKAGHDVMRDVRRRIQVIFQDPYSSLNPRMTVGQIISEPLLVYRMVPNKKAARERVE
                     DLLTQVGLYPYMADRYPHELSGGQRQRVGIARALAMEPKFIVCDEPVSALDVSIQAQV
                     "
BASE COUNT          401 a          680 c          732 g          426 t
ORIGIN      
        1 ggctgataat cagtcaaacg atttggcggg gccaattcgc gggcggacaa aaagcaggtc
       61 cgcggagtct ttgctgtttc ccagggaaca ggccctctcg tcgtcgttgc ccgtctcttc
      121 gaacagccgg atcgatgaac agccggaccg atcggcgagt tccaccgagg aaggacctgg
      181 cctggaaagg aacgcagggc gggggctggc ttccgtgcaa cttcgccgga agtttacatg
      241 ccgacgggtt gcccgatcgg ccgtaggacg ctatgcatgc ccacgatctc ggaacctcct
      301 gtgaaggcga gggattccgc gaggagtcgg agtttgttcc acgcctggta gaggcgagtg
      361 cggcgacgaa cttcgatttg caaaattggg aggatggcgg gtacgcaagt acgcgtcacg
      421 tcatgaggcg taatggccag cagtagtgac actcgcaccc aaaccagtcc cttcagcgat
      481 tttgtttctt tcggtgacac agtagagagc cgcaccgagc ggccgggttc ggcccagccg
      541 tgaccgcaga agtcattgct gattctgccc cccgcgtaga tgcggcagag gctgccgcgt
      601 cttccccctg gctgctggag gtcgaaaatc tgcacgttca tttcgtgacc actgccggcg
      661 tggtgcgggc agtcgaaggc ctgtcctaca aggtcaagcc cggtgaggtg gtggcgctcg
      721 tcggggagtc cggctgcgga aaatccgtgt cggcgctcac ggtcatgcgc ttgctcgcca
      781 agccggcggg ccgtgtcgtc gcaggccgta tcctgttcca agggcgtgat ctcctgaagc
      841 tcagcgaaga ggagatgcgc gcgatccgcg gccgggagat ctcgatgatc ttccaagagc
      901 cgatgacctc gctcaatccg gtgctcacga tcggcttcca gatcatggag ccgttgatga
      961 tccacctcgg catgagcgag aagcaggcgc gcgcccgcgc ggccgagctg atgcgcatgg
     1021 tcggcattcc ggacggcgaa cggcggctcg accaatatcc gcaccagttc tcgggcggca
     1081 tgcgccagcg cgtgatgatt gccatcgggc tcgcctgcaa tccgaagctc atcatcgccg
     1141 acgagccgac cacagcgctg gatgtgacga tccaggccca gatcctacag cttatgaagg
     1201 atctgtcccg cgagcttggc atcgcgatgc tcatcatcac gcacaacctg ggcgtggtgg
     1261 cgcgttacgc cgatcgcgtg aacgtcatgt acgcggcgcg catggcggag cagggcactg
     1321 ccgacgacgt gtttgcgcgg cctcgccatc cgtataccta cggacttttg cgttccgtgc
     1381 cgcggctcga cagaccgcgg gaagccaagc tcgaaacgat cgaagggctg ccgccgaatc
     1441 tagtttctcc gccgccaggg tgtcgctttg cgccccgctg tgcagcagcg accgaggcgt
     1501 gcaaggtggc tccaccacct ctcgtggagg tggagagcgg gcactattcg gcctgcattc
     1561 gggccaccga gctcgcagag aagggcccgc aggctattgg cctttcgaaa cctgccggcc
     1621 cggccaagct agaggtgcca cgctccgaaa gcccaccgct gctcaggttg gagcgcctga
     1681 cgaagcactt cgtagtgtcg tcgggctcga ggctttttgg caggtcgtct gcaattgtgc
     1741 gcgcggtcaa cgacgtcagc ttctcgatcg cgcctgggca gacgctcggc cttgtgggtg
     1801 agtccggctg cggcaagacc acggtgggcc ggctggtgct gaagctggag gagccgactt
     1861 ccggctcgat tttcttcgaa ggaaaagatt tgaccaaagc cggccacgac gtgatgcgcg
     1921 acgtgcgccg gcgcatccag gtcattttcc aggacccgta ctcttcgctc aacccgcgca
     1981 tgaccgtcgg ccagatcatt tctgagccgc tgttggtcta ccgtatggta ccgaacaaga
     2041 aggctgcgcg ggagcgcgtg gaggatttgc tgacccaggt cggcctctat ccgtacatgg
     2101 cggatcgcta tccgcatgag ctttccggcg ggcagcggca gcgcgtcggc atcgcgcgtg
     2161 ctctcgcgat ggagccgaag ttcatcgttt gcgacgagcc cgtctcggcg ctcgatgtgt
     2221 cgatccaggc gcaagtcat
//
LOCUS       DASUMG010000216         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_28461, whole genome shotgun sequence.
ACCESSION   DASUMG010000216 DASUMG010000000
VERSION     DASUMG010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28461"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(553..1293)
                     /locus_tag="VFX46_03595"
     CDS             complement(553..1293)
                     /locus_tag="VFX46_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599699.1"
                     /translation="MLTSVLEGSVSVGPYPELAGKRVLVTGLELQAGVDVARAFAEQG
                     CRIVLHTTASGAEADAVLEMLAQTASELQVYSDPLTDIESTTRFAQTAAKTFSGVDAV
                     VNLIHLDLRALSTEVLDGDFETAIARRLQTARVITRIAANRMRLTWSEGLVFNVLVST
                     PPQNNAETALLSMARAMLASMTRSEASEWADQAIRINAVAPVLDSTEAGACLVSEPQV
                     AALAMFLASSRGRDLSGLVFDLGLVEHA"
     gene            complement(1487..2035)
                     /locus_tag="VFX46_03600"
     CDS             complement(1487..2035)
                     /locus_tag="VFX46_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5599700.1"
                     /translation="MTVDVGGRLRYVRERYNLSQRELAKRSGVPNSTISLIEANKVNP
                     SIGALKRILDGVPIGLAEFFAIEPDKPRKIFYQAEELTEIGKGGISYRQVGENLVGRA
                     LQILKERYEPGSDTGRVPLVHEGEEGGIIISGRLEVTVDSERRILGPGDAYYFESRRP
                     HRFRCIGPVPCEVVSACTPPSF"
     gene            2245..>2762
                     /locus_tag="VFX46_03605"
     CDS             2245..>2762
                     /locus_tag="VFX46_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5599701.1"
                     /translation="MAPLDMKPNELESYWLPFTPNRSFKKKPRLFSGAKDLYFFTPDG
                     RKVIDASSGLFCVNAGHGREPIVKAIQEAAARLDYAPPFQYGHPQVFELSSRLALMAP
                     GDLEYTLFANSGSEAVDTALKVALAYHRARGEGSRTRFIGRERGYHGVGFGGISVGGM
                     VANRKMFGNMLTG"
BASE COUNT          530 a          853 c          847 g          532 t
ORIGIN      
        1 cagaggagga gcgcgacgcc tcccagcaat gtcaggagca gctcgcttcc ggccatcctc
       61 ttcttaatgc tccgtggaaa acgccaatgt gaaaattgaa cctttgaccg gtccagtcac
      121 atgcgcgaag cacgcagcat attctgcccc ggtccgaaaa cctagggacc ggcatcgatg
      181 cggcccgttg ccgaatccca gtgtccgccg cggtgaccgc ctcttgcaca agcagccggc
      241 ctcgcctgct aactcgtcaa aggtgaagca ggctcctcca atcgctacgc tgtaacgcta
      301 gatggctcac tgccaggatc attggcggtt acggcgccag cctgctggca agccgcagga
      361 cgcaaaatcc ccagtgcagc tccgtggtta ccgcgagcac gtgcacgaca tcattgatat
      421 cgctgatcgg accgcgatcg cgacaataca ttggttcgat atgggatcgc ggtcggttcg
      481 gtgccagggc ttctgtggtg agcgggaacc cgggcacaag gcccgggacg gctatccgcc
      541 agagccgtgc cgtcaggcgt gctcgaccaa cccaaggtcg aagactagtc ccgacaggtc
      601 gcggccgcgg gagctcgcaa ggaacatggc gagcgcagcc acctgtggtt ccgacacgag
      661 gcaagcgcca gcctcagtcg agtcgagcac cggggcaacc gcgttgatac gaatggcctg
      721 atcggcccat tcacttgcct cgctgcgagt catgctggct agcatcgcgc gggccatgga
      781 cagcagggct gtttccgcat tgttttgtgg cggcgtgctg acgagaacgt tgaaaaccaa
      841 cccttcgctc catgtcagcc gcattcgatt ggcggcaatg cgcgtgatca cccgtgcggt
      901 ctggaggcgg cgggcaatcg cggtttcgaa gtcgccatcc agcacctcgg tcgagagggc
      961 tctgaggtcg aggtggatca gattgacgac ggcatcgact ccggagaacg tcttggccgc
     1021 tgtctgcgcg aaccgggtgg tggattcgat gtctgtgagg ggatcggaat agacctgtaa
     1081 ttcggatgcg gtttgggcca gcatttccag cacggcatcg gcttctgccc cgctggcggt
     1141 cgtgtgcaac acgatccggc atccttgctc tgcgaaggcg cgggccacat ccacgcctgc
     1201 ctgaagctcc agtcccgtaa cgagaacgcg tttccccgcc agctcaggat agggaccaac
     1261 gcttacgcta ccctccagca cactcgtcaa catgagcgct tacctcctcg caccgtcggg
     1321 cctcccctgc ccgcacggcg ccactcaacg ccgattcgac taacgactcc taaatgacgc
     1381 caggattcgg gtgcggccgg gactgaaggg cggcgcaatt agggccgtac acagctgagt
     1441 tggatggtag cttcgtaatc gggcgaagtg aacggataag gtcggctcaa aacgatggcg
     1501 gcgtgcaggc gctgacgacc tcgcacggga ccgggccgat gcagcggaag cgatggggtc
     1561 ggcggctttc gaagtagtac gcatcacccg gccccaggat gcgccgttcg gagtcgaccg
     1621 tgacctctag ccggccgctg atgattatgc cgccttcctc tccttcatgc acaagaggca
     1681 cgcggccggt gtcagacccg ggctcgtagc gctctttcaa gatctgcaac gcccgtccca
     1741 ccaggttctc gcccacctgc cgatatgaga tgccgccctt tccgatctcg gtcagctcct
     1801 cagcctggta gaagattttg cgcggcttat ccggctcgat ggcgaagaac tcggctagcc
     1861 cgattggaac gccgtcgaga atacgtttca gggcgccgat ggacgggttt accttgttcg
     1921 cttcaatcag cgaaatcgtc gagtttggga cgccggaccg tttggccaat tcacgctgag
     1981 aaaggttata gcgttcgcgg acgtaacgca gacgcccgcc gacatcgacc gtcatggagc
     2041 tgccccgtgt ttcaagtgtt caatatttca aaaactctgc aatgcgggtg cagcgaaatc
     2101 aagtgtgtag aatttgggag aaaagggctt gatgggctat gacgatcggg cctctactcc
     2161 tattcaggcg ggcgtctgcg aatttgcctg gagtaccccc ggtggggcta ctgacgccct
     2221 gagcaacacg attggaggaa tgcaatggcg ccgctcgata tgaaacccaa cgagctcgag
     2281 tcctactggc tgccctttac gccgaaccgc tcgttcaaga agaaaccccg cctcttctcc
     2341 ggcgccaagg acctgtattt cttcacgccg gacggccgga aggtgatcga cgcctcgtcg
     2401 ggcctgtttt gcgtgaacgc cggacacggc cgcgagccga tcgtcaaggc tattcaggag
     2461 gcagcggccc ggctcgacta tgcgccgccg ttccagtacg gccatccgca ggtattcgag
     2521 ctttcatctc gtcttgctct gatggcgccg ggtgacctgg aatacactct gttcgcgaac
     2581 tcgggctctg aggctgtcga cacggcgctg aaggtggcgc tcgcctatca ccgcgctcgc
     2641 ggcgagggca gccggacccg cttcatcggc cgtgagcgcg gctatcacgg cgtcggcttt
     2701 ggcggcatct ccgtgggcgg catggtcgcc aaccggaaga tgttcggcaa catgctgacc
     2761 gg
//
LOCUS       DASUMG010000217         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1153240, whole genome shotgun sequence.
ACCESSION   DASUMG010000217 DASUMG010000000
VERSION     DASUMG010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153240"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VFX46_03610"
     CDS             <1..981
                     /locus_tag="VFX46_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5599702.1"
                     /translation="KIAGAMPCYLKGHSRGEYVFDWAWADAYERAGGSYYPKLLSAVP
                     FTPVPGPRLLVRPGPDRQSIERTLAAAAITLAKKLGVSSLHITFPTEGEWQRLPELGF
                     LQRTDQQFHWHNQGYGSFDDFLATLASRKRKAVRKERAQALAPGITIEHVMGADIREE
                     HWDAFFDFYMDTGSRKWGRPYLNRRFFSLLGEVMGDRCLLVMAKRRGRYIAGALNLIG
                     GDCLYGRYWGAIEHHPYLHFEVCYYQAIEYAIANKLPRVEAGAQGEHKLARGYMPVPT
                     YSLHWIADPSFRRAVARYLEEERREVAEHRDILAAYGPYRKVAPEAEETC"
     gene            1014..1439
                     /locus_tag="VFX46_03615"
     CDS             1014..1439
                     /locus_tag="VFX46_03615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX5599703.1"
                     /translation="MMSYDDSNVFAKILRGELPAHKVYEDEQTLAFMDIMPRGKGHTL
                     VIPKTPARNILDATPEQLAACMATVQRIARAVMKAFDAPGVTIQQFNESAGGQVVFHL
                     HFHVLPRWEGVPLGPHTGKMEDSEVLAANAEKIRQALAS"
     gene            1650..2294
                     /locus_tag="VFX46_03620"
     CDS             1650..2294
                     /locus_tag="VFX46_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599704.1"
                     /translation="MATILIVGASRGTGLETVRKALAAGHRVRALARSAANIPIEDAN
                     LEKITGDATDRTALLNAVDGVDAVIVTLGVPPSLSTAFSGTKLFSTATRALVDAMKEK
                     GVSRLIAITGIGAGESRGHGGPLYDWIAFPLLLKRVYDDKDVQEQIIRQSGLDWTIVR
                     PGLLTNGPTTGRYRALLDPSRWRADQISRADVADFLVKEVTERKFVHKAPVLLG"
     gene            complement(2298..3137)
                     /gene="soxA"
                     /locus_tag="VFX46_03625"
     CDS             complement(2298..3137)
                     /gene="soxA"
                     /locus_tag="VFX46_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HEX5599705.1"
                     /translation="MFACLSGLRIIAVALVTMIALLASGARATDPETLGSGEIIPESL
                     RPEDRRSGTTFLSESLRAQQADEGANPGMLWVATGESLWSTPAGPEGKSCASCHGDAE
                     TSMKGVAARYPMVDKATGELLNVELRINQCRRERQGAEPFAYESNELLGLTAFIAYQS
                     RGMPISVSVDGPAASHFEAGRKLFTMRQGQLNLACTQCHDENWGRKLRGDTISQGHPT
                     GFPIYRLEWQSLGSLHRRLRACSLGVRAEVLDYGSPEYLALELYLAWRTRGMPIETPA
                     IRR"
     gene            complement(3137..>3417)
                     /gene="soxZ"
                     /locus_tag="VFX46_03630"
     CDS             complement(3137..>3417)
                     /gene="soxZ"
                     /locus_tag="VFX46_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HEX5599706.1"
                     /translation="GEVVEIKTLIKHPMETGYRRDHRGQPIPRHIIQRFVATYNGEEV
                     FSADLTQGVAANPYLAFSLLVEESGEITFTWTDDLGSTHTEKAEITVA"
BASE COUNT          656 a         1064 c         1064 g          633 t
ORIGIN      
        1 aaaatcgccg gcgcgatgcc gtgctatctg aaaggtcaca gccggggcga gtatgttttc
       61 gactgggctt gggccgacgc ctatgagcgg gccgggggca gctactatcc gaagctcttg
      121 agcgcggtcc catttacgcc ggttccaggg ccacgccttt tggttcggcc gggacccgac
      181 cgacagagta tcgagcgtac gcttgccgct gctgcgatta ccctcgccaa gaaactcggc
      241 gtatcgtccc ttcacatcac atttccaacg gaaggcgaat ggcagcgcct gccggagcta
      301 ggctttctgc agcgcacgga ccagcagttc cattggcata accaaggcta cggctcgttc
      361 gacgactttc tcgcgacgct cgcctcgcgg aagcgcaagg cggtgcgcaa agagcgcgcg
      421 caggcactcg cgcccggcat cacaatcgag cacgtgatgg gggccgacat ccgcgaggag
      481 cattgggatg cctttttcga cttttacatg gacaccggct ctcgcaagtg gggccggcct
      541 tacctcaacc ggcgattctt ctcgctgctc ggcgaggtca tgggggacag gtgtctgctc
      601 gtcatggcaa agcggcgagg ccgctatatc gcgggagcgc tcaacctgat cggcggtgat
      661 tgcctctacg gccgctactg gggcgccatc gagcaccatc cgtaccttca cttcgaggtc
      721 tgttattacc aggccatcga gtacgcgatc gccaacaagc tgccgcgcgt tgaggcgggg
      781 gcccagggcg aacacaagct ggcccggggc tacatgcccg tgccaacgta ttccttgcac
      841 tggatcgccg atccgagctt tcgccgcgcc gttgctcgct atctcgagga ggagcgtcgg
      901 gaggtggcgg agcacagaga cattctggcc gcttacggac cctatcggaa agtcgctccc
      961 gaggccgagg agacgtgttg agattagttg gcgtccacag ctgaaaggac tggttgatga
     1021 gctacgacga cagcaacgtt tttgcaaaga ttctgcgggg tgagttgccg gcccacaagg
     1081 tctacgagga cgagcagacg ctcgccttca tggacatcat gccacggggc aagggccaca
     1141 cgctcgtcat cccgaagacg cccgcacgca acatcctcga cgctacacca gagcagctcg
     1201 ccgcttgcat ggctaccgtg cagcgtatcg cccgtgccgt gatgaaggcg ttcgatgcgc
     1261 cgggggtcac catccagcag ttcaatgaat cggcgggcgg ccaggtcgtt ttccatctcc
     1321 atttccacgt gctgccgcgt tgggaaggcg ttccgcttgg gccgcatacg gggaagatgg
     1381 aggacagtga ggtacttgcc gccaacgcgg agaagatccg gcaggcgctc gcctcataac
     1441 ggcatacgcg ctctgcgcca aagtgacgtg atcagaatgc ggcgcatcgt ctgcgaaccg
     1501 ttgcagtaga cggtgcgttt cagcgcagcg tccaggcgtc gaggcacaac gctcagaacc
     1561 ggcggcagcc gacctatgct cacctagcct gctgtaagca ccgtagaagc aagctcgacg
     1621 ctgccgcggg catgcgaaag gaggcgagga tggcgacgat actcatcgtc ggtgccagcc
     1681 gaggtacagg gctagagacc gttcgcaagg ctcttgccgc cgggcatcgg gtacgcgcct
     1741 tggcgcgatc agccgcaaac atcccgatcg aggacgctaa cctcgaaaag atcaccggcg
     1801 acgctactga ccgcacggcc cttctgaatg cggtcgatgg cgtcgatgcc gtgatcgtca
     1861 ccctcggtgt tccgccaagc ctctccacag ccttttccgg cacgaagttg ttctcgacgg
     1921 ccacgcgcgc cctcgtggac gccatgaagg aaaagggtgt gagccggctg atagccatta
     1981 caggcatagg ggccggtgag agccgcggcc acggtggccc gctctacgac tggatcgcct
     2041 tccctctcct gctgaagcgc gtttatgacg acaaggacgt gcaggagcag atcatccgcc
     2101 agagcggcct cgactggacc atcgttcgcc ccggtctcct gacgaacggc ccgacgacgg
     2161 gccgctaccg cgcactcctc gacccatctc ggtggcgcgc cgatcaaatc agccgggctg
     2221 acgttgcgga cttcctcgtg aaggaagtga ctgagcggaa gtttgtgcac aaagcaccgg
     2281 ttctcttagg ttgaggctca gcggcgtatt gcgggtgttt cgatcggcat gccgcgggtg
     2341 cgccacgcga ggtaaagctc caaggccagg tattccggcg agccgtaatc cagaacctcc
     2401 gcgcgcacac ccaggctgca cgcgcgcaaa cggcggtgaa gggagccgag agactgccac
     2461 tcgagccgat aaatgggaaa tcccgtcgga tggccttggc tgatggtgtc tcctctgagc
     2521 ttccggcccc agttctcatc atgacactgg gtgcaagcga ggttgagctg accctgccgc
     2581 atcgtgaaga gtttgcggcc cgcctcgaaa tggctggctg ccggtccgtc gaccgacaca
     2641 gagatgggca ttccccggga ctgataggcg atgaaggctg taagtcccag cagctcattc
     2701 gattcatacg cgaacggctc cgcgccctgc cgctcgcgtc ggcattggtt gatacgcagc
     2761 tcgacgttga gcagctcgcc ggtggcctta tcgaccatag ggtagcgcgc cgccacgcct
     2821 ttcatgctgg tttcagcatc accatggcag ctagcgcagg acttgccctc aggacccgcg
     2881 ggcgtggacc acagactctc ccccgtggca acccaaagca taccgggatt ggcaccctcg
     2941 tctgcctgct gtgcgcgaag ggactccgaa agaaacgtcg tgccggagcg ccgatcctcc
     3001 ggcctgagac tttctggaat gatctctccg ctgcctaagg tctctgggtc tgtggctcga
     3061 gcgccgcttg ccagcagcgc gatcatcgtc accagagcga cagcaataat ccgcaaacct
     3121 gataaacagg cgaacatcag gcgaccgtaa tttcggcctt ttccgtgtga gtgctgccga
     3181 gatcatcagt ccaggtgaag gtgatctcgc cgctttcctc gacgaggagc gaaaacgcaa
     3241 gatagggatt ggccgccacg ccctgcgtga ggtcggcgct gaacacctcc tcgccgttat
     3301 acgtcgcgac gaaacgctgg atgatgtggc gcgggatggg ttggccgcga tgatcccggc
     3361 gatagcccgt ctccatcggg tgcttgatca gtgtcttgat ttcgaccacc tcgccgc
//
LOCUS       DASUMG010000218         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_350506, whole genome shotgun sequence.
ACCESSION   DASUMG010000218 DASUMG010000000
VERSION     DASUMG010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350506"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1039)
                     /locus_tag="VFX46_03635"
     CDS             complement(92..1039)
                     /locus_tag="VFX46_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HEX5599707.1"
                     /translation="MSVKRKLASLAVGAAVALAGGTAQADQVRLMTGPQGGIWIPLGG
                     QLKDIWEKMIPGLQVQSLPGAGVANVRAIETGKAEVGFGNTITTADAIRGNEPFNEPH
                     KNVCNVASLYPQYFQAVVLAESGINSVKDLKGKAITTQPRGNTGELITQHWLKVHGLT
                     YSDVKMSFVSYTDSANQMKDKQAQLFTLGTGIPAASIMDIAAGVDIKLLDMSEDLEEM
                     RKLNPGYTLLEIPAGTYPKQDKPVKVIGYATHIVAACNLPEDTVYQMTKGIYENVDSL
                     SATSKAMAGLTPQKMAEDIGVPFHPGAAKFYKEHGIDVN"
     gene            complement(1373..>2177)
                     /locus_tag="VFX46_03640"
     CDS             complement(1373..>2177)
                     /locus_tag="VFX46_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5599708.1"
                     /translation="SVHGRMPSVTTGANLANRDLVYIGVSGDGDTASIGLGQFAHAVR
                     RRLNMVYIVMNNGCYGLTKGQFSATNDKTSPSKKGEPNPFDPIDLCQMAIQLGAGFVA
                     RSFSGDKRQLIPLIKAAISYRGFAFLDVISPCVTFNNHPASTKSYDYVRQHNQAIDKL
                     DFVPEEQEITADYDEGTSVPVTLHDGSRIILHKLDQSHDPRDADAALMTIRKHAARGE
                     VATGLLYIDESQHDLHDILRTSERPLNAVPMSELCPGSEKLASINARLQ"
BASE COUNT          424 a          637 c          651 g          465 t
ORIGIN      
        1 attgcgcact caactcagca ttgagtgctg cgggcatgaa gtgcaaaaag agtgatggag
       61 tgggccgcca tttttgacgg cccgaccaat gtcagttcac gtcgatgccg tgctctttgt
      121 agaacttggc ggcaccggga tggaacggaa cgccaatgtc ctcggccatt ttctgaggcg
      181 tcaagcctgc catggccttg gacgtagccg agagggaatc gacgttctcg tagatcccct
      241 tggtcatttg gtaaaccgtg tcctccggca gattgcaggc cgctacgata tgggtggcat
      301 aaccgatgac cttcaccggc ttatcctgct tcggataggt ccccgctggg atctcgagca
      361 gtgtgtagcc gggattgagc tttctcatct cctcgagatc ctcggacata tcgagcagct
      421 tgatgtcgac accggctgca atgtccatga tggacgccgc gggaatgccc gtgcccagcg
      481 tgaacagctg cgcctgcttg tccttcatct ggttggccga gtccgtatag gagacgaagc
      541 tcatcttcac gtcggaatag gtgaggccgt gaactttcaa ccaatgctgg gtaatgagtt
      601 cgcctgtatt ccccctcggc tgagtcgtaa tggccttacc tttgaggtct ttcacagaat
      661 tgattccgga ttccgccagc acgaccgcct ggaaatactg cgggtaaagc gaagcaacat
      721 tgcacacgtt cttgtgcggc tcgttgaacg gctcattgcc tcttatcgcg tcagcagtgg
      781 tgatagtatt gccgaaaccg acctcagcct tgccggtctc aatggcgcgc acgttggcga
      841 cacccgctcc aggcagtgac tgcacctgca gcccgggaat catcttctcc cagatgtcct
      901 ttagctgtcc gccgagcggg atccaaattc cgccctgagg accggtcatg aggcgaacct
      961 gatccgcttg agccgttccg cccgcgaggg caacggcggc acccacggct agagacgcaa
     1021 gtttccgctt cacagacatt ttggcgtttc ctttccgacg gttggcaaca attccgcccg
     1081 ccgcagctcc tgcagctgcg ggggaatgca catcgtgttg ttaggctgat atccgcgcgg
     1141 cagtactgct tggatcgcgc cgagtcgcgc ggtggctgcg caacattgcg cgcttcgctg
     1201 gccagcgtac catcccgagc tcctccacaa ggcccaaaac gggtcaatac ttattcgcgt
     1261 tattgtcgac gcgaagcgtt caggaactcg accacaaagt caagtacgca aacgccagcg
     1321 cgagatcgcg ctggcgtgaa gtcagaccga aaagaggaag tgcaaaacga ggctactgca
     1381 gccttgcgtt gatgctcgca agtttctccg agccggggca cagctccgac atcgggacgg
     1441 cgttcaacgg cctctccgac gtacgcagaa tatcgtgcag atcgtgctgg ctctcgtcga
     1501 tgtagagcag gccggttgcc acctccccac gggccgcgtg cttgcggatg gtcatcagcg
     1561 ccgcatctgc atcccgagga tcatgggatt gatccagctt atgcagaatg atgcgggacc
     1621 catcatgaag cgttaccggc accgaggtgc cctcgtcgta atccgccgtg atctcctgct
     1681 cctccggcac gaagtcgagc ttgtcgatcg cctggttgtg ctggcgcacg tagtcgtagc
     1741 tcttggtgga ggcgggatgg ttattaaacg tcacacacgg gctgatgacg tcgaggaagg
     1801 caaagccgcg gtagctgatc gccgccttga tgagcggaat gagttggcgc ttgtcgcccg
     1861 agaaggagcg ggccacgaag cctgccccca gctggatcgc catctggcaa aggtcgatcg
     1921 ggtcgaacgg attgggctcg cccttcttcg acggggaggt cttgtcgttg gtggccgaga
     1981 actggccctt ggtcagtcca tagcagccgt tgttcatgac gatgtagacc atgttcagcc
     2041 gccgccgaac ggcgtgcgcg aactggccga gaccaatcga ggctgtatcc ccatcgccag
     2101 aaacgccgat gtagacgagg tcgcgattgg cgaggttggc ccccgtcgtc acggacggca
     2161 ttcgcccgtg gacgctg
//
LOCUS       DASUMG010000219         8571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_751830, whole genome shotgun sequence.
ACCESSION   DASUMG010000219 DASUMG010000000
VERSION     DASUMG010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751830"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1346
                     /locus_tag="VFX46_03645"
     CDS             9..1346
                     /locus_tag="VFX46_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX5599709.1"
                     /translation="MSNLFQAAGLEKRPPQPLADKLRPKKLSEVVGQDHLVGPDGTLT
                     RMVRSGRLQSMIFWGPPGSGKTTVARLLANETALEFEQLSAIFTGVQDLRKAFDRAKA
                     RRAAGKGTLLFIDEIHRFNRSQQDSFLPYMEDGTITLVGATTENPSFELNAALLSRAT
                     VLVFHRLDEAALSELLRRAEAEEGRPLPVDQEAREALIAMADGDGRAVLNLAEEMFAA
                     VPVGAKPLTRDELTSLVQRRAPLYDKSRDGHYNLISALHKSVRGSDPDAALYWLARML
                     DAGEDPLYLARRLVRMAVEDVGLADPHAVVQALAAKDVYDFLGSPEGELALAQATIYL
                     AVAPKSNATYVAHKRAMRAAKDGGSLMPPKHILNAPTRLMAEVGYGAGYEYDHDTPEG
                     FSGQNYFPDGMERQQFYDPPERGFEREIRKRLAYFAELRRRRQERETKTSGAT"
     gene            1450..1998
                     /locus_tag="VFX46_03650"
     CDS             1450..1998
                     /locus_tag="VFX46_03650"
                     /inference="COORDINATES: protein motif:HMM:NF025478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system amidase effector
                     protein Tae4"
                     /protein_id="HEX5599710.1"
                     /translation="MVTFRDLWYGHPINESVQSPCIAPRDLTNLEGTSVARGFPVFAN
                     QCAIRMGVALKRAGVTANQLPGCAHCAVHPRDEMHFINATQLANAINRANLPGVGPRE
                     VISGPDAAKFYPKIFGRTGIIFIQDYWRRSFDPVGNPTGDHIDVWNGYRSSAKWLMEW
                     FSWLGYYSNYAEAREIWFWEVK"
     gene            2048..2278
                     /locus_tag="VFX46_03655"
     CDS             2048..2278
                     /locus_tag="VFX46_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599711.1"
                     /translation="MRYLIAMIFAIVAAAAATVFISSHVATWVVERMTFESPDEVANL
                     HDIVFMGVNLLALAIGWAIGWWLGNFERQSEL"
     gene            2503..2913
                     /gene="crcB"
                     /locus_tag="VFX46_03660"
     CDS             2503..2913
                     /gene="crcB"
                     /locus_tag="VFX46_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018328253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEX5599712.1"
                     /translation="MPAKGPVSGMMKYLLLASAGGALGAGARYLVYAEFTRRLGASFP
                     WATLTVNVVGSLLIGVLSALLLERFPDGAGLRVFIFTGILGGFTTFSAYSIEVISMLE
                     RGSFGSALTYAGSSVLLSLAACWVGLAGTRALIQ"
     gene            2910..3926
                     /locus_tag="VFX46_03665"
     CDS             2910..3926
                     /locus_tag="VFX46_03665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX5599713.1"
                     /translation="MKEAVVNEERTSDRVETLTVKPDESGMRLDRWFRSHFPQVTYTY
                     LQKLLRTGQVRVNSRRVQGGDRLEAGDQVRVPKVVRMPPKHGPSVVPPPGLSKADRDF
                     IERMILYEDDDVLVLNKPYGIAVQGGTGTKRHIDGLLAGMIDRFGDRPRLVHRLDRDT
                     TGVLLVAKRRDIAAKLGRVFQTRSAAKTYWALVKGVPKPPQGKVEAALVKTTGPEGDR
                     VRKAGPGEQDKAMHATTYYAVIDRVGTKAAWVSLKPVTGRQHQLRAHMSIIGHPIVGD
                     SKYEGGLDLPVEGIEPKLHLHARRLVIPHPRGGKIDVTAPLPDHMRRTWDLLGLDEGK
                     WSKE"
     gene            4026..4883
                     /locus_tag="VFX46_03670"
     CDS             4026..4883
                     /locus_tag="VFX46_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEX5599714.1"
                     /translation="MIGVFDSGLGGLTVLRALVKRFPQLSFVYLGDQAHVPYGNRPSD
                     QIVELTRAGVEHLFRQGCRLVILGCNTATAVALRRLQQEWLRSSGYSGHNVLGIVAPT
                     VEAATQTAWAVSTPQYPQKYKTDVIAVFGTQRTISSGVYPQEIRKRCPRVRVVQQACP
                     DLAGAIESARPESELERMVAEAVGSLMSTLEGETPHEAILGCTHYALIEHIFRRHLPG
                     STRILSQPEVVADSLEDYLTRHPEYATQDGSPPTLTLLTTGEPASTNELAQVFWPDAN
                     RFKRLSDFL"
     gene            5151..5354
                     /locus_tag="VFX46_03675"
     CDS             5151..5354
                     /locus_tag="VFX46_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599715.1"
                     /translation="MLRPLWIRSTAAIAIAPAGLGAGGGIVTSEETSGPLPAARGEER
                     DTGIVSGDAVNSSASGAGANFRK"
     gene            complement(5425..7620)
                     /gene="pnp"
                     /locus_tag="VFX46_03680"
     CDS             complement(5425..7620)
                     /gene="pnp"
                     /locus_tag="VFX46_03680"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX5599716.1"
                     /translation="MFDIHRVEIEWGGRKLRLETGQLARQADGAVLAQYGETTVLATV
                     VAEKEVRPGVDFFPLTVNYQERTYAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FVEGFKNETQVIATVLSFDNENDPDIVAMIASSAALTLSGVPFLGPIGAARVGLIDGE
                     LVLNPLIDEMEKTKLDLVVAGTQEAVMMVESEAHELSEDKMLEAVMFGHRHFQPVIQA
                     IIKLAETAAKEPWEFEIPDKSKYKDRVKELAEAELRQAYSITEKQKRREMVEAIRQKV
                     FAELLPPDDPADAVLLADVFKSLEQEIVRGNILKTGKRIDGRDLKSIRPIMAQVGVLP
                     RTHGSALFTRGETQALVVATLGTGEDEQYVDTLEGTKKERFMLHYNFPPFSVGETGRI
                     GSPGRREIGHGKLAWRALNPLIPSPEEFPYTIRVVSEITESNGSSSMATVCGSSLAMM
                     DAGIPLKRPVAGIAMGLIKENDSFAVLSDILGDEDHLGDMDFKVAGTSEGITSLQMDI
                     KITGITEEIMRVALEQAREGRMHILGEMAKALGEARPELGEHAPRIETIRIPTDKIRE
                     VIGTGGSVIRSIVEESGAKIDIEDDGTVRVAAPKREAIEAAIRRIREIASEPEVGEIY
                     KGKVVKIMDFGVFVNFFGSRDGLVHISQLTPKGRPNSPHEVVKEGQEVYVKLLGFDDR
                     GKVRLSMKVVDQQTGKEIPQEPRPEGEEGAEARADRRDRRDRRRDREAG"
     gene            complement(7851..8120)
                     /gene="rpsO"
                     /locus_tag="VFX46_03685"
     CDS             complement(7851..8120)
                     /gene="rpsO"
                     /locus_tag="VFX46_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX5599717.1"
                     /translation="MSITPERKQEIIREHATKPGDTGSPEVQIAILTERINQLTEHFK
                     VHKKDNHSRRGLLKMVGQRRQLLDYLKRKDEARYQRIIDKLGIRR"
     gene            complement(8313..>8571)
                     /locus_tag="VFX46_03690"
     CDS             complement(8313..>8571)
                     /locus_tag="VFX46_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599718.1"
                     /translation="SKLDAAMDFLKVKLLPVEAALEELQRINLSPADASRVRRGQSVL
                     IRGRDAPILSGEAYAMSKGQLVALGELTKGEFRPTRVFNLN"
BASE COUNT         1505 a         2644 c         2691 g         1731 t
ORIGIN      
        1 agaagcaatt gagcaatctc tttcaggcag caggactgga gaaacgaccg ccgcagccac
       61 tcgccgacaa gctgcggccg aagaagctga gcgaagtggt cgggcaggat catctcgtcg
      121 ggcctgacgg cacgctgacc cgcatggtgc gctccggccg cttgcaaagc atgatcttct
      181 ggggcccgcc gggctcgggc aagacgaccg tggcccggct gctggcgaac gaaacggcgc
      241 tcgagttcga gcagctctct gccattttca ccggtgtcca ggatctcagg aaggcgttcg
      301 atcgtgccaa ggcgcgccgc gctgccggca agggcacgct gctcttcatc gacgagatcc
      361 atcgcttcaa ccgctcgcag caggacagct tcctgcccta catggaagac gggacgatca
      421 cgctggtcgg cgcaaccacg gagaacccgt cgttcgagct caacgcggcg ttgctgtcgc
      481 gggcgactgt gctggtcttc catcggctcg acgaggcggc cttgtcggag ctgttgcgcc
      541 gcgcagaggc ggaggaaggg cgtccgttgc cggtcgatca agaggcgcgc gaagctttga
      601 tcgcgatggc cgatggcgat ggccgggcgg tgctgaacct tgcggaggag atgttcgctg
      661 cggttcccgt cggggccaag ccgctgacgc gcgacgagct gaccagcctc gttcaacgcc
      721 gtgcgccgct ctacgacaag tcccgcgatg gtcactacaa cctgatcagt gcgctgcata
      781 agtctgtgcg cggctctgat ccggatgcgg ccctttattg gctcgcgcgc atgctcgatg
      841 ccggcgagga cccgctgtat ctcgcccgcc gtctggtgcg tatggccgtc gaggatgtgg
      901 gcctcgcaga tccgcacgcc gtggtgcagg cgctcgcggc caaagatgtg tacgacttcc
      961 tcggcagccc cgagggcgag ctggccctgg cgcaggcgac gatctatctg gccgtggcgc
     1021 cgaagtcgaa cgccacgtac gttgctcaca aacgcgccat gcgggccgcg aaggatggtg
     1081 gcagcctcat gccgcccaag cacattctga atgcgccgac ccggttgatg gccgaggtgg
     1141 gttacggggc aggctacgag tacgatcacg acaccccaga aggattttcc ggccagaact
     1201 attttccgga cgggatggag cggcagcagt tctacgatcc tcccgagcgt gggttcgagc
     1261 gcgaaattcg caagcgcctc gcctatttcg cggaattgcg cagacgtcgt caggagcgag
     1321 agacgaagac gtcaggagcg acatagatct ctcgcgacag gcgcccttcg gatggcatcg
     1381 cgagactgtt cgtgccccgc gcaattggtg tagtgtgccc cctggcaaaa agcagtgggg
     1441 aggggcatca tggtcacgtt ccgtgacctt tggtatgggc acccgatcaa cgagtccgtt
     1501 cagtcaccgt gcattgcgcc acgtgacctg acgaacctgg aaggcaccag cgttgcccgt
     1561 ggtttccctg tattcgcgaa ccagtgcgct atccggatgg gagtggcgct gaagcgtgct
     1621 ggcgtgaccg cgaaccagct accgggatgt gcgcattgcg ccgtgcaccc gcgggatgag
     1681 atgcatttca tcaatgccac tcagctagcc aacgccatca atcgagccaa tcttcccggc
     1741 gtcggccccc gtgaggtcat cagcggtccc gacgcagcga agttctaccc gaagatattc
     1801 ggccgcaccg gtatcatttt catccaggac tattggcgcc gcagcttcga cccggtgggc
     1861 aatccgaccg gcgaccatat cgacgtctgg aacggctacc gctcgtccgc gaagtggctg
     1921 atggagtggt tctcttggct cggctactac tccaattacg ccgaggcgcg agagatctgg
     1981 ttctgggagg tgaagtgagc gagtgcaccg cgccgcggac gcatccacct gacaaggagc
     2041 acgcctgatg cgttatctca tcgcgatgat tttcgcgatc gtagccgcgg cagcggcaac
     2101 ggtgttcatc agctcgcacg tggcgacgtg ggtcgtggag cgcatgacct tcgagagtcc
     2161 ggacgaggtc gccaacctgc acgacatcgt cttcatgggc gtgaaccttc tagccctcgc
     2221 catcggatgg gcgattggct ggtggcttgg caatttcgag aggcaaagtg aattgtagtc
     2281 cggggccgtt ctgcctttgc tcttcagatc gtctccatct ataaggcggg cttgctccac
     2341 ccaagcgtcg tggtgaaagc accctggcgt gttctggcca gggccagcga tcgtgatccc
     2401 gcgtggcaat agggtctgtg gagccagact gcgcgcgatt gcccacggaa gaggggccgc
     2461 gtgggtgttt caagaactcc aaacttaagc tgaggattgc gggtgccggc caaaggccct
     2521 gtatcgggaa tgatgaagta tttgctgctc gccagtgccg gcggcgcgct cggtgcgggc
     2581 gcacgctatc tggtctatgc cgagtttaca cgccggttgg gtgcctcgtt cccctgggca
     2641 acgctgacgg tgaatgtcgt gggctccctg ctcataggcg tgctttcggc gctgctgttg
     2701 gagcgcttcc ctgatggcgc cggtcttcgg gttttcatct tcaccggcat tcttggtggc
     2761 ttcacaactt tttcggcgta ttcgatcgaa gtcatctcaa tgctggagcg aggatcattt
     2821 gggagcgcgt tgacttacgc cggtagctcc gtcctgcttt cgcttgcggc atgctgggtg
     2881 ggcctcgccg gaacgcgggc gctaatccaa tgaaagaagc tgttgtgaac gaagagcgca
     2941 caagcgatcg ggtcgagacg ctgaccgtca agccggatga gtccggcatg cggctcgacc
     3001 gctggttccg cagccatttt ccgcaggtca cctatacgta tttgcaaaag ctgctccgca
     3061 ccggccaggt gcgggtgaac agccgccggg tgcaaggcgg tgatcggctc gaggccggcg
     3121 atcaggtccg tgtgcccaag gtcgtgcgca tgccgccgaa gcacggcccc tctgtcgtgc
     3181 cgccgccggg cctctccaag gcggaccgcg atttcatcga gcgcatgatc ctctatgagg
     3241 acgatgatgt gctcgtcctc aacaagccgt acggcatcgc cgtgcagggc ggcacgggca
     3301 cgaagcggca cattgacggc ctgctcgcgg gcatgatcga ccgcttcggc gacaggccgc
     3361 ggctcgtcca ccgcctcgac cgcgacacga cgggcgttct cctcgtcgcc aagcgccgcg
     3421 acatcgccgc caagctcggc cgcgtgtttc agacgcgctc cgcagccaag acctactggg
     3481 cgctggtgaa gggtgtgccg aaacccccgc aggggaaggt ggaagcggcg ctcgtcaaga
     3541 ccacaggtcc cgagggggac cgcgtgcgga aagcgggccc tggcgagcag gacaaggcca
     3601 tgcacgcaac cacgtattac gcggtgatcg atcgtgttgg cacgaaggct gcgtgggtgt
     3661 ctctgaagcc agttaccggc cgccagcatc agctgcgcgc gcacatgtcg atcatcggcc
     3721 acccgattgt gggcgacagc aagtacgaag gcggcctgga cctaccggtg gaggggatcg
     3781 agcccaagct tcatctccac gcccgccgtc tcgtcatccc gcatccccgc ggcggaaaga
     3841 tcgacgtaac ggcaccgctg cccgatcaca tgcgccgcac gtgggactta ctggggctcg
     3901 atgaaggcaa gtggtcgaag gagtgaccag ctgggtcagg tttgggcctg aacttacctg
     3961 atcttgaccc cactcggaca tactcgtgtt gcagtctcgg gtgcggcgtg gcatggcgtc
     4021 tcaacatgat aggtgttttc gattccggcc ttggggggct gacggtgctg cgggcgctgg
     4081 tgaaacgctt tccgcagctt tcgttcgtct atctcggcga tcaggcccat gtgccttatg
     4141 gcaaccgccc ctccgaccag atcgtggagc tgacccgggc cggtgttgag cacctgttca
     4201 ggcaaggctg ccggctggtc atcctcggct gtaatacggc gacggccgtg gcgttgcggc
     4261 ggctgcagca ggaatggctc cggagttcag gatattcggg ccacaatgtt ctcggtatcg
     4321 tggcacccac ggttgaggca gcaacgcaga ccgcgtgggc cgtttcgaca ccgcagtacc
     4381 ctcagaagta caagacggac gtgatcgcgg tgttcggcac acagcgaacg atttcatccg
     4441 gcgtctatcc gcaagaaatc cggaagcggt gccctcgggt gcgggtggtg caacaggcct
     4501 gtcccgacct tgccggggcg atcgagagtg ccaggcccga gtccgaattg gagcggatgg
     4561 tggcggaggc tgtgggcagc ctgatgagta cgctcgaagg cgagacgccc catgaggcta
     4621 tcctcgggtg cacccattac gcgttgatcg agcatatttt ccggcgtcat ttgccgggat
     4681 cgacccgcat cctctcacag cccgaagtcg tggctgatag cctggaggat tacctgaccc
     4741 gacaccctga atatgcaact caggatggtt cgcccccgac gctcaccctt ttgaccacag
     4801 gagaaccggc ctcgaccaac gaattggcac aagtattttg gccggatgcc aatcgattca
     4861 agcggttgtc ggattttctt tgattccctt tccaataagc ttggaaccac tcgccatcaa
     4921 ttaagtattt ccatttacgc taattcaaaa ccgatcaaat tcagtaacca tcgaaacttc
     4981 tctttgcagt gacggttatt ccatcaggaa caatcattgt aagaagagag gtccgatgag
     5041 tcgtcgtcta atcattacgt gtgccggagc cgttcttggc ctcggcctcg ctctatccgc
     5101 cgggcaggcg gcgccagcca gccctggcca gttgaagggt gccaccaacg gtgctgagac
     5161 ccttgtggat caggtccacg gccgccatcg ctattgcgcc cgctggcctg ggcgcgggtg
     5221 gtggcatcgt cacgtcggaa gaaacttccg gcccgcttcc tgcggcccgc ggcgaggagc
     5281 gagatacagg cattgtttcc ggagacgcgg tgaactcgtc tgcgtctggc gctggcgcta
     5341 atttccgcaa gtaaacgagc tcaacaaaaa ggggccgggg gaaccttccc tccagcccct
     5401 ttcacatgtg caacccgctc atgatcagcc tgcctcgcga tcgcggcggc gatcccgccg
     5461 gtcgcggcga tctgcccgcg cttcggctcc ttcctctccc tcggggcgcg gctcctgcgg
     5521 gatttccttg ccggtttgct gatcgacaac cttcatggac aagcgcacct tgccgcgatc
     5581 gtcgaagccc aggagcttca cgtagacttc ctggccttcc ttgacgacct catgcggtga
     5641 gttcgggcgt cctttcggcg tcagctgcga gatgtgcaca agaccgtcgc gggagccgaa
     5701 gaagttcacg aacacgccga agtccatgat cttcacgacc ttgcccttgt agatctcgcc
     5761 gacctcgggc tcggacgcga tttcgcggat gcgccttatt gcggcttcga tcgcttcgcg
     5821 cttgggcgcc gccaccctca cggtcccgtc gtcctcgatg tcgatcttgg cgccgctctc
     5881 ctcgacgatg gagcggatga cggagccgcc ggtgccgatc acctcacgga tcttatctgt
     5941 cggaatacgg atggtctcga tgcgcggggc atgctcaccc agctcggggc gagcctcgcc
     6001 aagggccttc gccatctctc caaggatatg catccggccc tcgcgcgcct gctcgagcgc
     6061 gacgcgcatg atctcctcgg tgatgccggt gatcttgatg tccatctgca gcgacgtgat
     6121 gccttcagag gttccggcaa ccttgaagtc catgtcgccg agatggtcct cgtcgccgag
     6181 aatgtccgag agcaccgcga acgagtcgtt ctccttgatc aaacccatcg cgatgccggc
     6241 caccggccgc ttcaagggaa tgcccgcatc catcatcgcg agcgacgatc cgcagacggt
     6301 tgccatcgat gacgagccgt tgctctccgt gatctcggac acgacgcgga tcgtgtaggg
     6361 gaactcctca ggggacggaa tgagcgggtt gagggcgcgc caagcgagct ttccgtgccc
     6421 gatctccctc cgccctggcg agccgatacg gcctgtttcg ccgacggaga agggcgggaa
     6481 gttgtaatgg agcataaagc gctccttctt tgtgccctcc agcgtatcca cgtactgctc
     6541 atcctcaccg gtgccgagcg tggccaccac gagcgcctgc gtttcaccgc gcgtgaacag
     6601 agcagatccg tgcgtgcgcg gcagtacgcc gacctgagcc atgatcggcc ggatcgactt
     6661 cagatcgcgc ccatcgatgc gctttccggt tttcaggatg ttaccgcgaa cgatctcttg
     6721 ctcgaggctt ttgaaaacat cggcgagcag aacggcatcg gctggatcat cgggaggaag
     6781 cagctccgcg aagaccttct ggcggatcgc ctccaccatc tcccgacgct tctgcttttc
     6841 ggtgatcgag taagcctggc gcagctccgc ttcggcgagc tcctttacgc gatccttgta
     6901 tttcgacttg tcagggatct cgaactccca cggctccttg gcggcggtct cggccagctt
     6961 gatgatggcc tggatcacag gctggaagtg cctgtggccg aacatgacgg cctcgagcat
     7021 cttatcctcg gagagctcgt gggcctcgct ctcgaccatc atgaccgcct cttgggtgcc
     7081 ggcgaccacg aggtcgagct tcgttttctc catctcgtcg atgagcggat tgagcacgag
     7141 ctcgccgtcg atcaggccga cacgggcagc gccgatcggc ccgaggaacg gcacgccgga
     7201 cagcgtgagc gccgccgacg aggctatcat ggcgacgatg tcggggtcgt tctcgttgtc
     7261 gaacgagaga acggtcgcga tgacctgcgt ctcgttcttg aagccttcga cgaagagtgg
     7321 ccggattggg cgatcgatca ggcgggaggt cagggtttcc ttttcggtgg ggcggccttc
     7381 gcgcttgaag tagccgccgg ggatttttcc tgctgcgtat gtcctctcct ggtagttcac
     7441 cgtcagcgga aagaaatcca ctcccgggcg gacttccttt tcggcaacaa cggtggccag
     7501 gacagtcgtt tcaccatatt gggcgagcac agcgccatcg gcctggcgag ccaattgccc
     7561 cgtctcgagc ctgagctttc gcccgcccca ttcaatttct acacgatgaa tgtcgaacat
     7621 cttgcgtctt tcgaacttga gtcgctctga acttgtgccg ccctcggcac aagcttccac
     7681 ttggagccat cgcgggagcg gacgccggac caaggcggtt caatcggctt aggcggcggt
     7741 ctcttcgagc tctctgccac cagcggtggt cctccaatgc atgccggccg ggccgacccg
     7801 gcgacgaata tacagcagac ggccttgttg ccgcctgctg tcttttttgc tcagcggcga
     7861 atgccgagtt tatcgatgat tctctggtag cgcgcctcgt ctttgcgctt caggtagtcg
     7921 agcagctgcc ggcgttgccc gaccatcttt agcagccctc gacgcgagtg gttgtccttc
     7981 ttgtggacct tgaagtgctc ggtgagctgg ttgatccgct ccgtcaagat tgcgatctgc
     8041 acctcggggg agccggtgtc cccgggcttt gtcgcatgct cccggataat ctcctgcttg
     8101 cgctcgggtg taatcgacat cgggttatct cctcatcctc aaagcttcac attgggtcct
     8161 gtgggaccgt ctgcggccac ggccgggatg tcgtccagca gggtcgcaaa gagaaacacg
     8221 ggcctgcttc gcccgtgctg ggccgttata cactaacagt ggtcaagggc gagcagtttc
     8281 tcgcggtcgc acggcggcat gcgagtgaaa aatcaattca ggttgaagac gcgcgtcggg
     8341 cggaactcgc ctttggtcag ctcgcccagg gcgacgagct gtcctttgga catcgcgtag
     8401 gcctcgccgc tcagaatggg ggcgtcccgg ccacggatca gaacgctctg gccgcgccgc
     8461 acgcgcgatg cgtccgccgg gctcaagttg atccgctgca gctcttcgag cgcggcctcc
     8521 acgggcagca gcttgacctt gaggaagtcc attgcggcgt ccagcttgct c
//
LOCUS       DASUMG010000220         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_350522, whole genome shotgun sequence.
ACCESSION   DASUMG010000220 DASUMG010000000
VERSION     DASUMG010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350522"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..848
                     /locus_tag="VFX46_03695"
     CDS             201..848
                     /locus_tag="VFX46_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX5599719.1"
                     /translation="MHAIDYLVHFSNILMLAAYSVRDILWLRWFAVAAALTNIPYFLL
                     QGTVLWPPVLWALVFTAINLYQIARLYLERRPVVLSQDEQKLYDLGFRSLRPREFVSL
                     SLVGEWKNAEPGERVITEGEPVSSLSIPITGSADVHSRGERIGALAPGHIIGTALALT
                     GEPSPVEVTFTEPARYMRWSLPSLRRFMDTRPDLRVTLQGLVNRDLAGKLERLMS"
     gene            999..>2141
                     /locus_tag="VFX46_03700"
     CDS             999..>2141
                     /locus_tag="VFX46_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5599720.1"
                     /translation="MSIARIKRMGLFGGPLLALLCYHLLPLQYSTGAGQWVEFSAAGR
                     PTLGMMVWMATWWITEAVDIEVTALLPIATFPLLGIAPLSKVLPSYAADVIFLFMGGF
                     IIGLAIERWGLDRRIAFFTLRLVGARPGAIVGGFMAVTAFLSMWVSNTACAAMMVPIA
                     LSVIDLVLRSRTGTSLKESGSIPHDCVPECNFAIGLLLCVAYSASIGGIATIIGSPPN
                     GIAVRYMQQTFQKEVSFFDWLLIGGPFTLIFLPIAWLLITRVLLRADIGEIAGGRQYF
                     DEEYRKLGPLTRGEKVVLVVFSLTALLWTCSPLLKSIVIADMKPLAGLSDTGIAMLAA
                     MALFLIPVDRANCVRAMDWRTAVKLPWGVLMLFGGGLTLAASIEANG"
BASE COUNT          393 a          625 c          652 g          471 t
ORIGIN      
        1 acacgcagac tggaaagatc ctctgggagt cggctgggga agtcaccgtg gcgacaaagc
       61 tcctaaggtc ggagcagacc gtgccccttg acggaatcgc gcagaagctg tggctgcgca
      121 tgatccagga taacttgctg gatggggaaa gtggatcacg atttaccttt agcaattaaa
      181 aacaaggaga ctagatcaat atgcacgcta tagactatct ggtacatttt tcgaatattc
      241 tgatgctggc ggcgtattcg gtgcgcgaca tcctgtggct gcgctggttt gccgttgccg
      301 ccgcgctcac caacatcccg tacttcctcc ttcagggcac cgtgctctgg ccaccggttc
      361 tctgggcact cgtattcacc gccatcaatc tctatcagat cgcgcgcctc tacctcgagc
      421 gacgtccggt agtgctctcc caggacgagc aaaagctcta tgatctggga ttccgctcgc
      481 ttcgtccccg cgagttcgtt tcactgtcgc tcgtggggga atggaagaat gccgagcccg
      541 gcgagagagt cataaccgaa ggagagccgg tgtcgtccct gagcattccg atcaccggaa
      601 gtgcagacgt ccacagtcgc ggcgagcgca tcggggcgct ggcaccaggc cacatcatcg
      661 gcactgcgct ggccctcacc ggagaaccat cgccggtgga agtgaccttc accgagcccg
      721 cacgttacat gcgctggtcg cttccaagcc tgcgtaggtt catggacacg cggcccgacc
      781 tgcgcgtgac gctgcaaggg ctggtgaacc gagatctggc cggaaaactc gaacgactga
      841 tgtcgtgaag gaacacaacc ggagaaaatc gagactatcc ctgaggggta gccgacgcgg
      901 cctcgaaact aatccagaag aatatttaat gcttcgccgc tgcttggcat ctcgtctttc
      961 acgtaaggtg atcgctggca gaggagaaaa ttgctggaat gagcattgcc aggatcaaac
     1021 ggatggggtt gttcggcggc ccgctgctgg cgctgctctg ttatcatctg ctgccgcttc
     1081 agtactcgac cggggccggg cagtgggtcg agttctcggc cgcgggccga cctaccctgg
     1141 gcatgatggt ctggatggcg acgtggtgga tcacggaagc cgtcgatatc gaggttacgg
     1201 cgctcctgcc catcgcaacc ttcccgctgc tgggcattgc gcccctgagt aaagtactgc
     1261 catcttacgc cgccgacgtg atcttcctgt tcatgggcgg ttttatcatt gggcttgcga
     1321 tcgagcgctg ggggctcgac cggcgcatcg ccttttttac tctccgactg gtaggcgcgc
     1381 ggccgggcgc gatcgtgggc ggatttatgg cagtcaccgc gttcttgagc atgtgggttt
     1441 ccaataccgc gtgcgcggcg atgatggtgc cgatcgcgtt gagcgtcatc gatcttgtct
     1501 tgcgcagccg caccgggacg agcctcaagg aatccgggag cataccccac gactgcgttc
     1561 ccgagtgcaa ttttgccatc ggtcttttgc tctgtgtcgc gtactcggca tcgatcggcg
     1621 gaatcgcgac catcatcggt tctcctccca acggtatcgc cgtgcgctat atgcaacaga
     1681 cgtttcagaa ggaggtgagc ttcttcgact ggttgctgat tggcgggcca ttcacgctga
     1741 tcttcctgcc gatcgcctgg ttgctgatta cgcgcgtctt gttgcgcgcc gacatcggcg
     1801 aaatcgccgg cggacggcag tatttcgatg aagagtaccg caagctgggc ccgctcaccc
     1861 gcggcgagaa ggtggtcttg gtcgtatttt ccctgaccgc gcttctctgg acctgcagcc
     1921 cgttgctcaa atcgatcgtc atcgcggaca tgaagccgct ggccgggctc tccgacaccg
     1981 gcatcgccat gctggcagcg atggcgctgt ttctgattcc ggtggatcgc gccaattgcg
     2041 tccgcgcaat ggattggcgc acggcagtga aactcccctg gggtgtgctg atgttgttcg
     2101 gcggcggcct cacgctggca gcttcgattg aagcgaacgg c
//
LOCUS       DASUMG010000221         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_993133, whole genome shotgun sequence.
ACCESSION   DASUMG010000221 DASUMG010000000
VERSION     DASUMG010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993133"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFX46_03705"
     CDS             complement(<1..388)
                     /locus_tag="VFX46_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX5599721.1"
                     /translation="MTSSLTFPDSQRQGGGFDRRKILTGAAALAASAVTIKTASAASV
                     APLGQTGAPTTATQPLPLGPLPGARYPDARLESMKKPTVSFGPTGFPAFAGTMAVERV
                     ATGFRWAEGPVYFPAGRYVLFSDIPNN"
     gene            786..1739
                     /locus_tag="VFX46_03710"
     CDS             786..1739
                     /locus_tag="VFX46_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5599722.1"
                     /translation="MTQTNMTTAGIDTSKAKLDIAVHGRTDRWQVANALPGWRALAAS
                     LTKAHVTRVGIEATGGYERGVVEYLRAAGLDVLVLQPIQVKAFGRVHLRRAKNDVLDA
                     VLIAACAAALDQVKIKPDQRLADLAGYLTFVEQIEEDIRRCKTRLEHIDELRLRRMVL
                     GDIARAKARRLAEIRRIARQLRAHPDLATRLELVLSIPGIGERTALALLIRMPELGCV
                     SREEAAALAGLAPFDNDSGQHKGQRRIAGGRARLRRSLFAAALPAAFRWNKALIALYR
                     RLTATGKAHNAALVACARKLLIYANTVVQRGTPWTERPIRV"
     gene            complement(1768..>2400)
                     /locus_tag="VFX46_03715"
     CDS             complement(1768..>2400)
                     /locus_tag="VFX46_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599723.1"
                     /translation="DGVVNNAGILRDMIFHKMSVEAFEAVIKVHLMGSFYVSHAAARI
                     FREQESGSFVHFTSTSGLIGNFGQANYAAAKLGIVGLSKSIALDMGRFNVRSNCVSPF
                     AWTRMIGTIPTETDAQKARVEKIKQMGPEKIAPVCAYLLSDAAQDVTGQIFGVRMNEI
                     FLFSQNRPLRSVHRAEGWTPQTIAEHGMPALKESFYKLDRSADIFPWDPI"
BASE COUNT          442 a          732 c          790 g          436 t
ORIGIN      
        1 ggttgttggg aatgtcggag aacagcacgt agcgcccggc cggaaagtag accggtccct
       61 cggcccagcg gaaaccggtc gcaacgcgtt cgacggccat ggtgccggcg aacgccggaa
      121 acccggtcgg gccgaacgaa accgtcggct tcttcatcga ctcaaggcga gcgtccggat
      181 agcgggcgcc ggggagcgga ccgagcggca ggggctgcgt cgccgtggtc ggcgcgccgg
      241 tctggccaag cggtgccaca gaggcggcag aagccgtctt gatcgtgacg gccgaggcgg
      301 caagcgccgc tgcgcccgtc agtattttgc ggcggtcgaa accgccacct tgtcgctggg
      361 aatcgggaaa ggtcagtgag cttgtcatga tttcctccca agttttttgt tggaaggaaa
      421 acatccgccc gcattcgggc ataaagcggg caagctacag cgaaacaacc ggagatggag
      481 caggatttgt agggtgggca aagcgcagcg tgcccaccac ttccgacgat ggtctcctat
      541 agatggtggg cacagcgctg cgcgcctttg cccacccttg taatagcgcg atcgattatc
      601 gttggtctgt tccgtgagga atggcctgcc gcggctgcaa cctgcggcag gccgctggcg
      661 atcggatcga gcccacccag agcagtttgt ggctgccccg tagcgtgatc gtcgtcagca
      721 ccttcatcgg acccggatgg ttgccggaac aacttggttc gcttcacaag gcagggtcgg
      781 cgaagatgac acagactaac atgacgacgg cgggaatcga tacgtccaaa gccaaactcg
      841 atatcgcggt tcacggtcga accgatcgct ggcaggtcgc caacgccttg ccgggttggc
      901 gtgctctggc agccagtctg accaaggccc acgtcacccg ggtcgggatc gaggcgacgg
      961 gcggctatga acgcggtgtg gtggaatatc tgcgcgcggc gggccttgat gtgctggtgc
     1021 tgcagccgat ccaggtcaag gcgttcggcc gggtgcatct gcgccgggcc aagaacgatg
     1081 tgctggatgc ggtcttgatc gcggcctgtg ccgcagcgct cgatcaggtg aagatcaagc
     1141 ctgatcagcg cctggcagat ctggctggat atctcacctt cgtcgagcag atcgaggaag
     1201 acatcaggcg ttgcaaaacc cggctcgagc acatcgatga gctcaggttg cgccgcatgg
     1261 tgcttggtga cattgcccgc gcgaaggcac gacggctcgc ggagattcgt cgcattgcca
     1321 ggcaattgcg cgctcacccg gatctggcta cccgcctcga gctggtgctc agcattcctg
     1381 gcatcgggga acgcaccgcg cttgctcttc tgatccgcat gcccgaactc ggctgcgtca
     1441 gccgggaaga agccgcagcc cttgcagggc tggctccctt cgataacgac agcggtcaac
     1501 acaagggcca gcgtcgcatc gctggcggcc gagcgcgctt gcggcggtcc ttgttcgccg
     1561 ccgccctgcc ggccgccttc cgctggaata aggcgcttat tgccctctac cggcggctga
     1621 ccgcgaccgg aaaggcccac aacgccgccc tcgttgcctg tgcccgcaag cttctgatct
     1681 atgccaatac ggtcgtccaa cgcggcacgc cgtggaccga acggcccatc cgagtttaat
     1741 ggttgctaca gccgcgcctc gtgttgctca gatcggatcc cacgggaaga tgtcggcgga
     1801 gcgatcgagc ttgtagaacg actccttgag cgccggcatg ccgtgctcgg cgatggtctg
     1861 cggtgtccag ccttcggcac ggtgcaccga gcgcagcgga cggttctggc tgaacaggaa
     1921 aatctcgttc atgcgcacgc cgaagatctg cccggtcacg tcctgcgccg catcggagag
     1981 cagataggcg caaacgggcg cgatcttctc cggacccatc tgcttgatct tctcgacgcg
     2041 tgccttttgg gcatccgtct cggttgggat ggtgccgatc atgcgggtcc aggcgaacgg
     2101 ggacacgcaa ttcgagcgca cattgaagcg gcccatgtcg agcgcgatcg acttcgacag
     2161 gccgacgatg ccgagcttgg cggcggcgta gttggcctgg ccgaaattgc cgatcaggcc
     2221 ggaggtcgag gtgaagtgca cgaaggagcc gctctcctgc tcgcggaaga tacgcgcggc
     2281 ggcgtggctg acatagaacg agcccatcag gtgcaccttg atgacggcct cgaacgcttc
     2341 cacgctcatc ttgtggaaga tcatgtcacg caaaatgccg gcattgttga ccacgccgtc
//
LOCUS       DASUMG010000222         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_190051, whole genome shotgun sequence.
ACCESSION   DASUMG010000222 DASUMG010000000
VERSION     DASUMG010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190051"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFX46_03720"
     CDS             complement(<1..930)
                     /locus_tag="VFX46_03720"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5599724.1"
                     /translation="MKAAVEVLDEIFVRHRPAAQALADWGKAHRFAGSGDRAAIGNLV
                     FDVLRRRLSLAAQMSDDSTRALVLAAAPEALAMRVEEVAAAVDGSDYALEPLSPSERA
                     GLEREVPEEAPLHVRADIPEWLAPSFERVFGDRLAEEGRALARRAPVDLRVNTLKADR
                     ERVLKALSRFGAEPTPISPIGVRIPPPTGPGRTPNVEAEPGHGKGWFEIQDEASQIAA
                     LLSGASPRRQILDLCAGAGGKTLAMAAAMQNTGQVYAYDADRVQLRPIFERLKRAGVR
                     NAQVLPAGDEASVRALSAHFDIVFVDAPCTGSGV"
     gene            complement(952..1074)
                     /locus_tag="VFX46_03725"
     CDS             complement(952..1074)
                     /locus_tag="VFX46_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599725.1"
                     /translation="MSRVIHRTIEVVRWGGFVAFFIALVAMWVLLLWRTLQVGL"
     gene            complement(1109..>2102)
                     /locus_tag="VFX46_03730"
     CDS             complement(1109..>2102)
                     /locus_tag="VFX46_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX5599726.1"
                     /translation="ITVKSGVDREEAKRLLHQYRIEKLLVVDDDYRCIGLITVKDIEK
                     AALFPNACKDEQGRLRVAAATTVGDEGHERTERLIAAGCDVIVVDTAHGHSRRVLDAV
                     TRIKRLSNSTQVIAGNVATAEATRALIDAGADAVKVGIGPGSICTTRVVAGVGVPQFT
                     AIIDCVNEARKSGIPVIADGGVKFSGDITKALAAGADAVMIGSLLAGTDESPGETYLY
                     QGRTYKAYRGMGSVGAMARGSADRYFQQEVKDTLKLVPEGIEGQVPYKGPVATVLHQL
                     VGGLRAGMGYVGAASIAELQAKAKFVRISQASVRESHIHGVGITRESPNYPGNG"
BASE COUNT          391 a          692 c          645 g          374 t
ORIGIN      
        1 aacgcccgac ccggtgcagg gggcatcgac gaacacgata tcgaagtggg cgctcagcgc
       61 gcgcacagac gcttcgtcgc ccgcggggag gacttgcgcg ttgcgcacac cggcgcgttt
      121 cagtcgctcg aagatggggc ggagctgtac gcgatcggca tcgtaggcgt agacctgccc
      181 cgtgttctgc atcgcggcgg ccatagccag cgtcttgccg cctgccccgg cgcagagatc
      241 tagaatttgg cggcgcggcg aggccccgga gagaagcgca gcgatctggc tcgcctcgtc
      301 ctggatctcg aaccagcctt tgccgtggcc cggctcggcc tcgacattgg gcgtgcggcc
      361 aggtccagtg ggcggcggaa tgcggacgcc aattggggag atgggcgttg gctcggcgcc
      421 gaagcgcgag agcgccttca gcacgcgctc acgatcagct ttcagagtat tcacccggag
      481 atcaacaggt gcccgacgcg ccaaggccct gccctcttcg gcgaggcgat cgccgaacac
      541 cctctcgaac gatggcgcca gccattcggg aatgtcggcg cggacatgaa ggggagcctc
      601 ttctggcacc tcgcgttcga gccccgcgcg ctccgatggc gagagcggtt cgagcgcata
      661 gtcggagccg tcgaccgctg ccgcgacctc ctcgacccgc atggccagag cttcaggcgc
      721 agcggcgaga acgagcgcac gagtggaatc gtcgctcatt tgtgccgcaa gcgagagccg
      781 gcggcgaagg acgtcgaaca ccagatttcc gatggccgcg cgatcgccag agccggcgaa
      841 gcgatgcgcc ttgccccaat cggccagggc ctgtgctgcg ggccggtggc ggacgaagat
      901 ttcgtccagc acctcgactg cagctttcat tcgggcaccc ggccgcatcg actacaaccc
      961 cacttgcaga gtgcgccaga ggagcagcac ccacattgcg acaagagcga tgaagaaggc
     1021 gacaaatcct ccccagcgga ccacctcgat ggtcctgtgg atcacacgcg acaccacgaa
     1081 gtccaagcga acgtgcgaca actgcagatc agccgttgcc tgggtagttc gggctttctc
     1141 gcgtgatgcc aaccccatgt atatggctct cgcgaacaga ggcttgagag atgcgaacga
     1201 acttggcttt tgcctgcagc tccgcgatgc tggcggcgcc aacgtagccc atcccagcgc
     1261 gcaatccgcc gacgagctgg tgcagcaccg tagcaaccgg tcccttgtag ggtacttggc
     1321 cctcgatacc ctccggtacg agtttcaggg tatccttcac ctcttgctgg aagtagcggt
     1381 ccgcgctgcc gcgggccatg gccccaaccg atcccatgcc gcggtaagct ttgtatgtgc
     1441 gcccctggta gaggtaggtt tctccggggc tctcatccgt gccggccaac aacgagccga
     1501 tcatcacggc gtcagcgcca gctgcaagag ctttggtgat atccccggag aactttacgc
     1561 cgccgtccgc aatgaccggt atgccagact ttcgcgcctc atttacacaa tcgattatag
     1621 cagtgaactg ggggacgcct acgcctgcca caacgcgcgt ggtgcagatg gaacctggtc
     1681 caatcccaac cttcaccgca tccgcgccgg catcgatcag cgcccgtgtc gcctctgcag
     1741 tggctacatt gccggcaatg acctgagtgc tgttcgagag tcgcttgatc cgagttactg
     1801 catcgagcac gcgccgcgaa tggccgtgcg cggtgtcgac gacgatcacg tcacagccag
     1861 cagcgatgag tcgctccgta cgctcgtgtc cctcgtcacc cacggtcgtc gctgccgcga
     1921 cccgcagccg gccctgctca tccttacacg cgttgggaaa aagcgccgcc ttctcgatgt
     1981 ctttgacagt gatgagacca atacagcggt agtcgtcatc tacgacgagc agcttctcga
     2041 ttcgatactg atgcagaagc cgctttgctt cctcgcgatc cacgccggat ttaaccgtga
     2101 tc
//
LOCUS       DASUMG010000223         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_511557, whole genome shotgun sequence.
ACCESSION   DASUMG010000223 DASUMG010000000
VERSION     DASUMG010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511557"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VFX46_03735"
     CDS             complement(<1..278)
                     /locus_tag="VFX46_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599727.1"
                     /translation="MITHIAVWITQAVSIREMAVRLLLVLSFFLFSLAQAVAEEPTKA
                     IADCNQFEDRARQIKGCTEFIRLGKALGPNLAVAYTNRGIAYAAQGNF"
     gene            443..1291
                     /locus_tag="VFX46_03740"
     CDS             443..1291
                     /locus_tag="VFX46_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5599728.1"
                     /translation="MKLVRFGAPGAEKPGIIDADGVIRDVSAHVSDFDGPNLSPASLD
                     KLRKLDLKSLPAAPAGVRLGPPVARTMNFIAVGLNYEDHARETNMPIPAEPILFNKLA
                     NCIIGPNDDVMIPKGSQKLDWEVEIAFVVGTRARYVEEKDALNYIAGYTICNDVSERY
                     FQIERGGQWMKGKCFETSGPLGPWLVTPDEIEDVQNLDMWLDVNGKRMQIGNTRTMIF
                     SIKYLLHYISQFMVLEPGDVVTTGTPPGVGMGMNPPVFLKAGDVMKLHIQGLGEQTQR
                     VVPFKE"
     gene            complement(1376..>2012)
                     /locus_tag="VFX46_03745"
     CDS             complement(1376..>2012)
                     /locus_tag="VFX46_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599729.1"
                     /translation="AAPLIDGGSLHPAMTTVEAPIARERQLPHEPRNGTAKESDDAWR
                     EEVLVTLLDGLNLPLESYTRLAEHKVAEAAVAAGRALYRMASDNLHLRKALIDRRRDW
                     EARVENVRRRLLDECPGQPAKLAIVDETARSVADLIPALLLSPEAGFLDELIEHLEAA
                     AGADALVQPAEQNLLMQLRDLQSFDLASETPWTHARVLIEHFETGSASPLN"
BASE COUNT          387 a          609 c          604 g          412 t
ORIGIN      
        1 ttgaaattgc cctgcgcggc ataggcgatg ccgcgattgg tgtacgccac ggccaggtta
       61 gggccgagcg ccttcccaag acgaataaat tcagtacacc ctttaatttg ccgggccctg
      121 tcctcgaatt gattgcaatc cgcgatggct ttggtgggct cttcggctac cgcctgggcg
      181 agggagaaga ggaagaacga cagcacaagg agcagtctca cggccatctc ccggatcgat
      241 acagcctggg tgatccagac cgctatatgg gtgatcatga ccgattcttc gagctccccc
      301 caccccggag cttgggcttc gccaacccat agtttgcctt gacacgccag tctttgacgg
      361 cgcgcacacc actttacgct cggtcgtaat cggcctaccg atagagttta tcacaacagc
      421 agcgctctag gagtgaaacc tcatgaagct tgtgcgattt ggcgcgcctg gtgcagaaaa
      481 gcccggcatc attgacgccg atggtgtcat tcgtgatgtc tcggcccatg tcagcgattt
      541 cgatggcccc aacctcagcc cggcgagcct ggacaagctg cgcaagctcg acctgaagtc
      601 gctgcctgcc gctcctgccg gtgtgcgcct tggtccgccc gtggcccgga cgatgaactt
      661 cattgcggta ggtctcaatt acgaggacca cgcaagagaa accaacatgc ccatcccggc
      721 agagccgatc ctgttcaata aactcgcgaa ctgcatcatc ggtcccaacg atgacgtcat
      781 gatccccaag ggctcgcaga agctcgattg ggaggtggag atcgctttcg tcgtcggaac
      841 ccgggcgcgt tacgtggaag agaaggacgc cctcaactac atcgccggct acacgatctg
      901 taacgacgtg tccgagcgct acttccagat cgagcgcggc ggccagtgga tgaagggcaa
      961 gtgcttcgag acgtcgggcc cgctcgggcc gtggctggtg acgccggatg agatcgagga
     1021 cgtgcagaac cttgacatgt ggctcgacgt caacggcaag cgcatgcaga tcggcaacac
     1081 tcggacgatg atcttctcga tcaagtatct gctgcactac atctcgcaat ttatggttct
     1141 ggagccgggt gacgtggtga cgacgggtac gccgccgggc gtcgggatgg gcatgaatcc
     1201 gcccgtcttc ctgaaagccg gcgacgtgat gaagcttcac atccagggcc tcggcgagca
     1261 gacccagcgg gtcgtgccgt tcaaggagta atactgagcc agtcgatgtt tccgatcccg
     1321 tgtgcgggcg accgtgcacg ggatcgtctt ttagggctcc ggcttctcga tatcgttagt
     1381 tcagtggcga ggcgctgccg gtttcgaaat gctcgatgag gacgcgcgcg tgcgtccagg
     1441 gcgtttcgct ggcgagatcg aaggactgca agtcgcgaag ctgcatcagg aggttttgct
     1501 ccgcaggttg caccagagca tccgcgcccg cggcggcttc gagatgttcg atcagctcgt
     1561 cgagaaaacc tgcctcgggg cttagcagca gggctggaat cagatctgca accgaacgcg
     1621 ccgtttcatc cacgatagcc aacttcgccg gctgtcccgg acattcgtcg agcagcctcc
     1681 tgcgaacgtt ctcgacccgc gcttcccaat cgcgacgcct gtcgatcaga gctttacgca
     1741 ggtggagatt gtcgcttgcc atacgataga gcgctcgccc cgctgcgacc gctgcctcgg
     1801 ccaccttgtg ttcggccagt cgggtgtacg actccaacgg taggttcagc ccgtcgagta
     1861 acgtcaccag aacttcttcc cgccacgcat cgtcactttc ttttgccgtc ccgttacgcg
     1921 gttcgtgcgg tagctgccgc tcgcgggcga tcggtgcctc gaccgttgtc attgccgggt
     1981 gcagcgaacc gccgtcgatg agcggagctg ca
//
LOCUS       DASUMG010000224         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_350729, whole genome shotgun sequence.
ACCESSION   DASUMG010000224 DASUMG010000000
VERSION     DASUMG010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350729"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="VFX46_03750"
     CDS             <1..427
                     /locus_tag="VFX46_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5599730.1"
                     /translation="VRMMMGAQAVFEPAKPRFGVRIVEGAPEPSRMTPARKRAIEIAS
                     DGQARAKSALAAEAGCSTAVIDGLIEAGVLVEVAIPEKRYPVPRPSHSTVQFGPEQER
                     AVHALRSAVDANNFSVTLLDGVTGSGKTEVYFEAVARAL"
     gene            440..1891
                     /gene="priA"
                     /locus_tag="VFX46_03755"
     CDS             440..1891
                     /gene="priA"
                     /locus_tag="VFX46_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX5599731.1"
                     /translation="MLIMLPEIALTSQFLSRFERRFGCKPVEWHSALASAERGRVWRA
                     AATGEARVVVGARSALFLPYRELGLIIVDEEHDQSYKQDDRVHYQARDMAVVRGRLGR
                     CAVVLSSATPSIETYVNARTGRYRHLVLPSRYSGTALPDIEAINLRKHPPERGRWLSP
                     VLVEAITETAAKGQQTLLFLNRRGYAPLTLCRTCGTRIECPQCSAWLVEHRFRRKLTC
                     HHCGFALPVPEKCPKCGDSESLVACGPGVERIAEEVMERFPSARLTLLSSDLVPTLTE
                     MRKVIAAIENREVDIIIGTQIVAKGHHFPDLATVGVVDGDLGLGQADPRAAERTFQLL
                     HQVTGRAGRSFAQGRGFVQTYMPDHPVMQAIISGDREAFLDREVSQRQAATLPPFGRL
                     AALIISARQKEVAEDYARQVVRRAPRAERIEVLGPAEAPLAIIRGRHRWRILVKSPRD
                     VDLQTYLRAWLSAVPDPKGDLRLTVDIDPYNFL"
     gene            2121..2681
                     /locus_tag="VFX46_03760"
     CDS             2121..2681
                     /locus_tag="VFX46_03760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEX5599732.1"
                     /translation="MATDEPILAGVAGRYASALFELAQEERQLEQVEQDLTTFQQLLN
                     ESEDLRRLVRSPVFSSDEQLAAISAVLDRVGIGGLAGNFLKLAARNRRLFALPDMIKA
                     FHAMCARARGEVEADVISAFPLKDEQMQELKNALKGSVGKDVKVHLKVDPKLLGGLVV
                     KIGSRMIDSSLRTKLNSLKIRMKEVG"
     gene            2681..>4013
                     /gene="atpA"
                     /locus_tag="VFX46_03765"
     CDS             2681..>4013
                     /gene="atpA"
                     /locus_tag="VFX46_03765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX5599733.1"
                     /translation="MDIRAAEISAILKEQIKNFGKEAEVSEIGQVLSVGDGIARVYGL
                     DTVQAGEMVEFPNGVRGMALNLETDNVGVVIFGPDTGIREGDTVKRTGAIVDVPVGKE
                     LLGRVVDALGNPIDGKGPIKATERRRVDVKAPGILPRKSVHEPMATGLKAIDALIPIG
                     RGQRELIIGDRQTGKTAIILDTFLNQKPLNQGDDESQKLYCIYVAVGQKRSTVAQFVK
                     VLEERGALEYSIIVAATASDPAPMQYLAPFAGCTMGEFFRDNGMHAVIIYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNDENKAGSLTALPVIETQANDV
                     SAYIPTNVISITDGQIFLETDLFYQGIRPAVNVGLSVSRVGSSAQTKAMKKVAGRIKG
                     ELAQYREMAAFAQFGSDLDATTQRLLNRGARLTELLKQPQFSPLKMEEQVCV"
BASE COUNT          736 a         1255 c         1299 g          723 t
ORIGIN      
        1 ggtgcggatg atgatgggcg cgcaggcggt cttcgagccg gcgaagcctc gcttcggcgt
       61 ccgcatcgtc gagggcgcac ccgagccttc ccggatgaca ccagcgcgga agcgggccat
      121 cgaaattgcc agcgatgggc aggcacgcgc aaaatccgcg ctggcagcgg aggcgggctg
      181 cagcactgct gtgatcgacg ggctgatcga ggcgggcgtg ctcgtcgagg ttgccattcc
      241 cgagaagcgc tacccggtgc cgcggccgtc gcacagcacg gtccagttcg ggccggagca
      301 ggagcgggcg gttcacgccc tgcgctcggc cgtcgacgcg aacaattttt ccgtgacctt
      361 gctcgacggc gtcactggtt caggaaagac cgaagtctat ttcgaagccg tcgccagggc
      421 gctgtagcgg aaccggcaag tgctgatcat gctgccggaa atcgccctga cgagccagtt
      481 cctcagccgc ttcgaacgac gctttggctg caagcccgtc gagtggcatt cggcgctcgc
      541 ctcggcggag cgtggccggg tctggcgtgc cgcggcgaca ggcgaggcgc gggtggtcgt
      601 aggtgcgcgc tcggcgctat tcctgccgta ccgtgagctg ggcctcatca tcgtcgatga
      661 ggagcacgac cagagctaca agcaggacga ccgggtgcat tatcaggccc gcgacatggc
      721 ggtcgtgcgc ggccgcctgg gacgatgcgc ggtcgtgctc tcctccgcga cgccctcgat
      781 cgaaacttat gtcaatgccc gcacagggcg gtaccgccac ctcgtattgc cgagccgcta
      841 ttccggtacg gcactgcctg acatcgaggc gatcaactta cggaagcacc cccccgagcg
      901 gggccgctgg ctctcgcccg tgctggtcga ggcgattaca gagacagccg ccaaagggca
      961 gcagacgctc ctgttcctca accggcgcgg ctacgctccg ctgacgcttt gccggacctg
     1021 cggcacgcgc atcgaatgcc cgcagtgctc cgcatggctc gtggagcacc gcttccggcg
     1081 caagctcacc tgccaccatt gcggatttgc actgccggtt cccgagaagt gccccaaatg
     1141 cggcgactcg gaatcgctcg tcgcttgcgg cccgggtgtg gagcgaatcg ccgaggaggt
     1201 gatggagcgg tttccttcgg cccgcctcac actgctctcg agcgacctgg tgccaactct
     1261 taccgagatg cgcaaggtga tcgcggctat cgagaatcgc gaggtggaca ttatcatcgg
     1321 gacacagatc gtcgcgaagg gtcaccattt tcccgatctc gcgaccgtcg gggtggtcga
     1381 cggcgacctt ggcctcggac aagccgatcc gcgcgcagcc gaacgcacct tccagctatt
     1441 gcatcaggtc acaggccgag ccggccgctc gttcgctcag ggtcgcggat tcgtgcaaac
     1501 ctacatgccc gaccatccgg tgatgcaggc gatcatctcc ggcgaccgcg aggcctttct
     1561 ggatcgtgaa gtcagccagc gccaggcagc gaccttgccg ccgttcggtc ggttggcggc
     1621 actcattatt tctgcgcgcc agaaggaggt ggctgaggac tacgctcgcc aggtcgtccg
     1681 ccgggcaccg cgcgcagaga ggatcgaggt gctgggcccg gcagaagcac ccctcgcgat
     1741 catccgcgga cggcatcgct ggcgcatcct ggtcaaatca ccccgagacg tggatctgca
     1801 aacgtacttg cgggcgtggc tgtcggcggt gccggatccg aagggagatc tccggttgac
     1861 ggtcgatatc gatccttaca atttccttta gaagaagctc gcattgggcc tatccgcgcg
     1921 cgagccgggc agagacgaaa tgcggcattg agagcccatt ccggcggttg atggcctgcg
     1981 cctgccgtgt tagaggggac ccggcttctg ggaggggtac gaagcggtgc ggcgtgccct
     2041 ttacagatct gccttcgccc gcgggtgacc gccgcgcggc cactgctttc tatttcctca
     2101 acgcgacgag gccgttcgac gtggcgaccg acgaaccgat attggcaggc gtagcagggc
     2161 gctacgcatc ggcacttttc gagctggccc aggaggaacg gcagctcgag caggtagagc
     2221 aggatctcac gaccttccaa caactcctga atgaaagcga ggatttaagg cgcctcgtac
     2281 gcagtccggt cttctcgagc gacgagcagt tggcggctat ctccgccgtg ctcgaccgag
     2341 tgggaatcgg tgggctcgca ggcaacttcc ttaagctcgc ggcgcgtaac cggcgcctgt
     2401 tcgccttgcc ggatatgatc aaggcattcc acgcgatgtg cgccagggct cgcggcgagg
     2461 tcgaggccga cgtcatttct gccttcccgc tgaaagacga gcagatgcaa gagctgaaga
     2521 acgctctgaa gggatccgta ggcaaagacg tcaaggtgca cctgaaggtg gatcccaagc
     2581 ttctcggcgg gctcgtcgtg aagatcggca gccggatgat cgactcgtcg ctgcgcacga
     2641 aactcaattc actcaagatt cgcatgaaag aggttggctg atggacatcc gggccgctga
     2701 gatctccgca atcctcaagg agcagatcaa gaacttcggc aaagaggccg aggtctcgga
     2761 aatcgggcag gtgctttcgg tcggcgacgg catcgcgcgc gtttacggcc tcgacaccgt
     2821 gcaagccggt gagatggtcg agttcccgaa tggcgttcgg ggtatggcgc tcaacctcga
     2881 gaccgacaac gtcggtgtcg ttattttcgg cccggacacc ggcatcagag agggcgacac
     2941 ggtcaagcgg accggcgcta tcgtcgacgt gcccgtgggc aaggagctgc ttggccgtgt
     3001 ggttgatgcg ctaggtaacc ccatcgacgg caaagggccg atcaaggcta cggagcgccg
     3061 gcgtgtggac gtgaaagcgc ctggcattct ccctcgcaaa tccgtgcacg agccgatggc
     3121 cacgggcctc aaggccattg acgcgctcat tcccatcggt cgcggacagc gcgagctcat
     3181 catcggcgac cgtcagaccg gcaagaccgc catcattctc gacactttcc tgaaccagaa
     3241 gccgctgaac caaggcgacg acgaatcgca gaagctctac tgcatctatg ttgccgtcgg
     3301 tcagaagcgc tcgacggtcg ctcagttcgt gaaggtcctg gaggagcggg gcgcgctcga
     3361 gtactcgatc attgtggctg cgacggcctc cgatccggcg ccgatgcagt atctggcgcc
     3421 gttcgccggc tgcacgatgg gtgagttctt ccgcgacaac ggcatgcacg ccgtcatcat
     3481 ctacgacgac ctgtcgaagc aggccgtcgc ctatcggcag atgtcgctgc tactgcgccg
     3541 tccgccaggc cgcgaggctt atccgggcga cgtcttctat ctgcattcgc gcctgcttga
     3601 gcgcgccgcc aagatgaacg acgagaacaa ggctggctcg ctaaccgcgc tgccggtcat
     3661 cgagacccag gccaatgacg tgtccgccta cattccgacc aacgttattt ccatcaccga
     3721 cgggcagatc tttctcgaga ccgatctgtt ctatcagggc atccgtcctg cggtgaacgt
     3781 cggcctttcg gtgtcgcgcg tcggctcttc cgcccaaacc aaggcgatga agaaggtcgc
     3841 gggccgtatt aaaggcgagt tggcccagta ccgggagatg gcggcgttcg cgcaattcgg
     3901 ctcggatctc gacgcgacaa cgcagcgcct gcttaatcgc ggtgcgcgac tgaccgagct
     3961 tctcaagcag ccgcaattct caccgttgaa aatggaagag caggtctgcg tga
//
LOCUS       DASUMG010000225         3036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1073160, whole genome shotgun sequence.
ACCESSION   DASUMG010000225 DASUMG010000000
VERSION     DASUMG010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073160"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VFX46_03770"
     CDS             complement(<1..346)
                     /locus_tag="VFX46_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599734.1"
                     /translation="MLGSLLLLIAVTVPVAIYAWRKNRNTLPRATMFARQEVSRILVR
                     LPFALFAASCIAELVPQRAIAAVLGSEAGFTGIIAASLLGGLMPGGPIVSFPIAILFA
                     HQGAGGPQVIALI"
     gene            complement(346..951)
                     /locus_tag="VFX46_03775"
     CDS             complement(346..951)
                     /locus_tag="VFX46_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX5599735.1"
                     /translation="MTTEQGQLQQEDSAGDPPHRVVDGALWFFVLLAAGSALLLVWFR
                     GFDALYTAVLRSATLLLTVAPMIVVGLLLGGLVKELSDPKQIAPVLGANSGIKGLALA
                     TALGAATPGGPFAAFPIVYALALAGADVGAVVAYLTAWSVLGLHRVIIWELPLMGLDF
                     VTARVLASLPLPLVSGLIARLLMRFIPELPIGRTHVKGGGE"
     gene            1275..2096
                     /locus_tag="VFX46_03780"
     CDS             1275..2096
                     /locus_tag="VFX46_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5599736.1"
                     /translation="MPRLVSQFLSAVVVLSAFLAAILPAHAVAPDQCLALAETPSRSL
                     VQKAALRIAQLKPSEVRLTYIGHSTFLIESAAGVKIATDYNDYVRPREVPDVITMNRA
                     HDTHYTHFPDPRVEHVLRGWNPEGGPALHDFKLHDVRIRNVPTNIRTWDGGTLPYGNS
                     IFVFEIAGLCIAHLGHLHHTLTTQQLAQIGQMDVVLVPVDGSFTLDLDGMLEVLRALK
                     APLVIPMHYFSSFTLNRFLSRIEDSFEVIRSEVPTAVISRATLPRNPQVLVLPGH"
     gene            complement(2134..>3036)
                     /locus_tag="VFX46_03785"
     CDS             complement(2134..>3036)
                     /locus_tag="VFX46_03785"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5599737.1"
                     /translation="LRDRLVHVYKAGILIGISTSDVGYRGRRTPTGVFALAERKVTNG
                     GDFSWTGYALHADHVRGYPASLGCIRLPSAFARLLDSVAGPGTLVILARQRTEPVDVV
                     HSGTLFPAVPIYEAGRLVRTVAVRSMPELLTGSPVSGHVAIVVSRSGGNAILLRDGLR
                     EHIARVTFAHPDRRIGTHVYSLTGTSLGGHELVWLAFGIGRDRREPHLVSWHGDEILG
                     QISFEDRAEAMAMARLLHTGATLVITDELATSRKHQTPEDFVLISTQGSKPQPSYQRR
                     RRRRRARATRRRQRWASALFTAWR"
BASE COUNT          604 a          971 c          935 g          526 t
ORIGIN      
        1 tgatcagcgc gatcacctgc gggccgccgg cgccttggtg agcaaacaga attgcgatag
       61 ggaacgaaac gatcggacct cccggcatga gacccccgag cagcgacgcc gcgatgatgc
      121 cggtgaatcc cgcctcgctc cccagcacag ctgcaatcgc ccgctgcggc accagctcgg
      181 cgatgcacga cgcggcgaag agcgcaaacg gcagccgcac taggatgcga gagacttcct
      241 ggcgcgcaaa cattgtcgcg cgcggcagcg tgttcctgtt cttgcgccaa gcgtagatcg
      301 caacgggcac agtcacggcg atcagaagga ggagcgaccc gagcatcact cgcctccgcc
      361 tttgacgtgg gtgcgcccga tcggcagctc tggaatgaaa cgcatcagca ggcgggcgat
      421 cagccccgat acgagcggca acggaaggct cgccaagacg cgcgccgtca cgaaatcgag
      481 ccccatcagc ggaagctccc aaatgatgac gcgatgcagg ccgagcactg accaggccgt
      541 gagataagcg acgaccgcac caacatcggc gcctgccagc gccagcgcgt agacgatggg
      601 gaaggcggcg aacggacctc ccggcgtggc agcacctagc gccgtcgcca gcgccagccc
      661 ctttattccc gaattggcgc caagaacggg agcgatttgc ttcgggtccg acagctcctt
      721 tacgaggcca ccgagcaaca ggcccacgac gatcatcggc gcgacggtca agagcagcgt
      781 cgcagatctc aagacggctg tgtaaagcgc atcaaagcct ctgaaccaga cgagcagtag
      841 cgccgagccc gcggcgagca gcacgaaaaa ccacaacgca ccgtcaacaa cacgatgcgg
      901 agggtcgcct gcgctgtctt cttgctgaag ctggccttgt tcggtcgtca tgattgccgc
      961 gccatgtttg gcagatacgg gcgcggctgt cacgatgcgc tggccacgtg tcgctattct
     1021 catccggtca ctggcagcga cctgaccggg gagcagacga cttcttgctc atgatcatct
     1081 ctataccggg ggaaatggag aagaccgctg ctcctcaccg gtcaggtcgt gccgaacata
     1141 ggctcacttc ttcctgcgca ctgttccgca aggaacattg aggccaacaa agcatcttct
     1201 tgctctcgca gggcgcgttg gcgtcacgag ccgcatgaat taggatgctt gcagacgaac
     1261 ggcaggagcg acgcatgccc cggctcgtat cgcaattcct ttcggctgtt gttgtgctgt
     1321 cggcattcct ggctgcgatt ttgcccgccc atgccgtggc ccctgaccag tgccttgcac
     1381 ttgccgagac accttcgcgc tccttggtgc aaaaggccgc gctgagaatc gcgcagctca
     1441 agccttccga agtgcgtctg acctacatcg ggcattcgac gttcctgatc gaaagcgcgg
     1501 ccggcgtgaa aatagcgacg gactataatg actatgtgcg ccctcgcgag gtgcccgatg
     1561 tcatcaccat gaaccgggcc cacgatacgc attacacgca ttttcccgat ccccgcgttg
     1621 aacatgtgct gcgcggctgg aaccccgagg gcgggcctgc tctacatgac ttcaagcttc
     1681 acgacgtgcg gatccgcaac gtgcccacga atattcgcac gtgggacggc ggcacgctgc
     1741 cttacggcaa ttcaattttc gtgttcgaga ttgccggcct gtgcatcgcc catcttggac
     1801 atctccatca cacgctgacg acgcaacagc tggcgcagat tggacagatg gatgtcgtcc
     1861 tggtgccggt ggacggaagc tttacgctcg acctcgatgg catgctggag gtgctccggg
     1921 cactcaaggc tcctttggtc attccgatgc actatttctc gagcttcacg ttgaaccgct
     1981 tcttgtctcg cattgaagac agtttcgaag tgatcagaag cgaggtgccg accgcggtca
     2041 tctctcgcgc gactttgccg aggaatcccc aggtcctggt gctaccaggc cattgacgcg
     2101 gcggatcact ttagtcggga tgacaaagct gccttagcgc caggcggtga aaagcgcact
     2161 cgcccacctc tgccgccgcc gtgtggcacg cgcccgccgt ctccgccgac ggcgctggta
     2221 cgacggctgc ggcttcgagc cttgcgtcga gatcagaacg aagtcttcgg gcgtctggtg
     2281 ctttcggctg gtcgccagct cgtccgtgat gacgagcgtg gcgcctgtat gaagcagcct
     2341 ggccattgcc atcgcttcag cgcgatcctc gaatgaaatc tgaccgagga tctcgtcacc
     2401 gtgccatgag accaggtgcg gctcgcggcg atcgcggcca atgccgaacg ccagccagac
     2461 cagctcgtgc ccgccgaggg atgtgcccgt gagagagtag acgtgcgtgc caatccggcg
     2521 gtcgggatgg gcgaatgtca cccgcgcgat gtgctcccga agcccatcgc gcagcaggat
     2581 tgcgttaccg cccgagcgcg acacgacgat cgcgacgtgg cccgatacgg ggctccccgt
     2641 cagcagctcc ggcatggagc gcacggcaac ggtccgcacc aacctgcccg cttcgtagat
     2701 cggcaccgcc ggaaagaggg tgccggaatg gacgacatcc acgggctcgg tgcgctggcg
     2761 tgcgagaatg accagggtcc ctggacccgc gacgctatcg agaagccgag cgaaagcgga
     2821 tggcagcctg atgcagccga gcgaagccgg atagcctcgg acgtggtcgg catgcaaagc
     2881 gtaaccggtc caggagaaat cgccgccgtt ggtcaccttg cgctccgcca gcgcgaaaac
     2941 ccctgtcggg gtacggcgtc cgcggtagcc gacgtcagaa gtcgagatgc cgataaggat
     3001 gcctgccttg tagacgtgaa ccagccggtc ccgcag
//
LOCUS       DASUMG010000226         3292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_430507, whole genome shotgun sequence.
ACCESSION   DASUMG010000226 DASUMG010000000
VERSION     DASUMG010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430507"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFX46_03790"
     CDS             complement(<1..954)
                     /locus_tag="VFX46_03790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5599738.1"
                     /translation="MRALTAAGYDVRLVSRLRSFSQEPDPGVYQDLKSTAGAEAWRLV
                     ADWSAPNADWRPDCWFTYHPYYKSPDWMGPEVCARLGLPYFTAEASYAAKRDEEPWAI
                     WQADVVSAVRNAAASFCFTARDREGLESLAPLAGPLVDLPPFIDPSQIPERQRRPSRA
                     APRIGVAAMMRPGNKLESYVVLSQVLQMLLDLPWRLAIMGDGPARADVLSAFSPIPPD
                     RIDWMGELEPEAVVDALGACDLYVWPGIGEAYGLAYLEAQCMGLPVVAQDTGGVSAVV
                     RPNETGWLTPPGDLGAFADAVRRLLLDADLRNAMGDAASRFV"
     gene            1175..>3292
                     /locus_tag="VFX46_03795"
     CDS             1175..>3292
                     /locus_tag="VFX46_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5599739.1"
                     /translation="MDPNLFRFIWHHSRREQIGILLLILASLPFYFASLDVPRIIVSE
                     ALQGGAFPKGSLTARVFDWTVTLPEFLGGGKIVLSEGVELDQISYLLALSFIFLTLVL
                     INGAFKYVINIRKGVLGEQILRRMRYQLFATLLRFRPEDIRSVKPSEAASMIKDEVDP
                     IGGFVGDAFIQPVFLGSQALTALFFIMIQSSWLGLIALAVVMVQAVVIPYLRREQIRL
                     GRERQVAARHLAGRIGEVVEAAPAVHIHGTAVFDKAEVGRRLSQLFAIRVALFKRKFA
                     VKYLNNLLAQATPFFFYAIGGYFALRGALDLGQLVAVLAAYRDLPPPVKELIDWDQQR
                     IDVAVKYQQVVSQFTVDKLLPEPRDERPEDFAGPICTEGLRVLDRRGSPLLFNVSLTI
                     ERPSHVALTGGNGSGRGTFAKVLGRQITEYEGSVRIGSRELRDMADETAGRLIAYAGP
                     DPLLFVGSIRDNVTYSLRRVPPVPASSCESRARQLDELLLNGDEIHLDDSWIDYDAAG
                     VSSAEELDAAVLRGLAVAGLDEEVYWFGLNGRLGPGTNSEIAERFVEARRRVREQLAA
                     QSLDDLVEPFDPDRYNVHASIGENLLFGVPVGHGPTRSELAADPYVRAILEAEALDEP
                     LIEVGLRIAELTAEIFADLPPGDPLFERYSLIRADEMEHYRKLLDIVRQPGGKAHLSP
                     GARTRLIALALGYIEPRHRLSLLD"
BASE COUNT          576 a         1081 c         1006 g          629 t
ORIGIN      
        1 gacgaaacgg cttgcggcgt cacccatggc attgcgtaga tcggcatcga gaagcagccg
       61 acgaaccgca tctgcaaagg cgccgagatc gccgggcggc gtgagccagc ctgtttcgtt
      121 aggacgcacg acggccgaaa ctccgccggt gtcctgcgcc acaaccggca gtcccatgca
      181 ttgggcttcg agataagcta ggccgtaggc ctcacctatg cctggccaga cataaagatc
      241 gcatgccccc aaagcgtcca caacggcctc gggctccagc tcgcccatcc aatcgatgcg
      301 atccggcggg attggcgaga acgcggagag cacatcggcc cgggctggtc cgtcgcccat
      361 gatagccagc cgccagggca gatcgagcag catctgcagc acctggctga gcacgacata
      421 gctttcgagc ttattgccag ggcgcatcat cgctgcgacc ccgatgcgcg gtgccgctct
      481 cgatggccgc ctttgcctct ccgggatttg cgatgggtct atgaaaggag gcaaatctac
      541 gagagggccg gcaagcggcg ccaggctttc cagcccttct ctgtctcgcg ccgtaaagca
      601 gaagctcgcc gccgcattgc gaacggcaga gacgacatcg gcctgccaga tggcccacgg
      661 ctcctcgtcg cgtttcgcag catatgaggc ttcagccgtg aaatagggca gtccaagccg
      721 agcgcatacc tccgggccca tccagtcggg cgatttgtaa tacggatgat aggtgaacca
      781 gcagtccggc cgccaatccg cattcggagc ggaccagtcg gccacaagcc gccaggcctc
      841 tgcgcccgcc gtcgacttga gatcttggta aacgcctgga tcgggctcct gagagaagct
      901 gcgtaggcgg ctcacgagac gcacatcata gcccgctgct gtgagtgctc tcatgagaag
      961 cgccgccatc agccgctccc ccgacggcgt cggatggtca ggtggggcca gtggagcata
     1021 gaacgcgacc ttcatgttac cccccgactg ttaactcgcc tccctaactc agccctgtat
     1081 tccgctgatc gcgctccaaa attgacacgc tgctgcttgc cccttgctgg cctcctcact
     1141 accttcggtg gtcgagagcg caggatttcg cgtgttggac ccaaacctct tcaggtttat
     1201 ctggcatcac agccgtcgcg agcagatcgg catccttctg ctgatcctgg cctcgctgcc
     1261 gttctacttt gcctctctcg acgtcccccg catcattgtg agtgaggccc tccaaggcgg
     1321 cgccttcccg aagggcagcc tcaccgctcg tgtcttcgac tggacggtta cgttgccgga
     1381 gttcctcggc ggcggcaaga tcgtgctgag tgagggggtc gagctcgacc agatctcgta
     1441 tcttctggca ctcagcttca ttttcctgac gctcgtcctc atcaacggcg ccttcaagta
     1501 cgtcatcaac atccgcaagg gcgtcctggg cgagcagatt ctgcgccgca tgcgctacca
     1561 gctgttcgcc acgcttctga ggttccggcc cgaggatatc cgttcggtta agccatccga
     1621 ggcggccagc atgatcaagg atgaggtgga tccgatcggc ggcttcgtcg gagatgcctt
     1681 catccagccc gtcttcctcg gctcccaggc gctcacggcg ctcttcttca tcatgattca
     1741 gagcagctgg ctgggcctca tcgccctcgc tgtcgtgatg gtccaggcag tcgtcatccc
     1801 ctacctcaga cgcgagcaga tccggctagg ccgcgagcgg caggtggccg cgcgccatct
     1861 cgccggacgc atcggcgagg tcgtggaggc ggcccccgcc gtgcacatcc acggcacggc
     1921 ggtgttcgac aaggccgagg tcggccggcg cctcagccag cttttcgcga tccgcgtggc
     1981 gctgttcaag cgcaagtttg ccgtcaagta cctcaacaac ctgctggccc aggcgacgcc
     2041 ctttttcttc tatgcaatcg gcggctattt cgcgttgcga ggcgcgctcg atctcggcca
     2101 gctcgtcgcc gtccttgccg cataccggga cctgccgccg cccgtgaaag agttgatcga
     2161 ctgggatcag caacggatcg acgtggccgt gaagtatcag caggtcgtct cgcaattcac
     2221 ggtcgacaag ctgctgcccg agccccgcga cgagcggccg gaggactttg ctggcccgat
     2281 ctgcaccgag gggctcaggg tgctcgaccg ccgcggatct ccgctgctat tcaatgtttc
     2341 gctcaccatc gagcggcctt cgcacgtcgc cctcacggga ggcaacggca gcggccgtgg
     2401 gacgttcgcc aaggtgctcg gccggcagat caccgagtac gagggcagcg tccgcatcgg
     2461 atcgcgggag ttgcgggaca tggccgacga gacagccggc cgcctcatcg cctatgcagg
     2521 gcccgatccg ttgctgtttg ttgggtcaat aagggacaac gtcacctatt cattgcggcg
     2581 cgttccacca gtgccagcgt cgtcttgtga aagcagagca cgccagctcg acgagctgct
     2641 gttgaacggt gacgaaatcc atctcgacga cagttggatc gactatgatg ccgctggtgt
     2701 cagctcggcg gaggagctgg atgctgccgt tctgcgcggt ctggcagtcg ccgggctcga
     2761 cgaggaggtg tactggtttg ggctgaacgg tcgtctcgga ccggggacca actctgaaat
     2821 tgcggagcgc tttgtcgagg cgcgacggcg agtccgagag cagcttgcgg cgcagtctct
     2881 cgatgacctg gtcgagccct tcgatcccga ccgctacaac gtccacgcct cgatcggtga
     2941 gaacctgttg ttcggcgttc cggtcggcca cggtcccacc cggagcgagc tggcagccga
     3001 tccctatgtc cgcgcgatcc tagaggcgga agcgctcgac gaaccgttga tcgaggtcgg
     3061 cttgagaatt gcggagctga ccgcggagat attcgccgac ctgccgcctg gggacccgct
     3121 cttcgagcgc tactcgctca tccgggctga cgagatggag cactatcgca agctcctcga
     3181 tatcgtccgg caaccagggg gaaaggcgca cctgtccccc ggcgcgcgca cccgactgat
     3241 cgctctcgct ctcggataca tcgagccgcg acaccgcttg agccttctcg at
//
LOCUS       DASUMG010000227         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_672228, whole genome shotgun sequence.
ACCESSION   DASUMG010000227 DASUMG010000000
VERSION     DASUMG010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672228"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VFX46_03800"
     CDS             <1..692
                     /locus_tag="VFX46_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5599740.1"
                     /translation="LGANTDPYQPIERQYRITRSVLEVLDRFSHPVSIVTKSALVVRD
                     IDILARMAERDLVKVALSVTTLDRRIARKMEPRATTPWRRLDAIKQLSAAGVPTQVMV
                     APIVPAINDSEIERILEAAHDAGARDSGYVLLRLPLELKELFREWLETEFPDRAKRVI
                     SLLQSMHGGRDYTPEFGLRQRGSGPYAEQIAMRFRLALKRLGMNERGLKLRTDLFQKP
                     EKSSGEQLQLL"
     gene            725..1402
                     /locus_tag="VFX46_03805"
     CDS             725..1402
                     /locus_tag="VFX46_03805"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX5599741.1"
                     /translation="MGGTESEPRPTFEVEAVAIRLTGGPVAGIDEAGRGPLAGPVVAA
                     AVILDPDNIPPGIADSKALEPDVREELFERIKATSKIGIGIADVARIDRYNILNATLW
                     AMQQAVKQLEPTPRVALVDGNRPPKLRCQTQCIVQGDTKSLSIAAASIVAKVTRDRIM
                     IELGRAYPGYGFERHKGYPTPEHFEAIDRLGVTPHHRRSFRPVQVALGLVPPEEAELP
                     LLGFNQA"
     gene            1506..>2635
                     /locus_tag="VFX46_03810"
     CDS             1506..>2635
                     /locus_tag="VFX46_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX5599742.1"
                     /translation="MVLRHRVRPSLSLDQVIVGDCLEELAKLPTASVDLVFADPPYNL
                     QLEKDLLRPNNTRVDGVDDEWDKFTDFEEYDRFSRAWLSECRRVLKRDGAIWVIGSYH
                     NIFRLGVALQDLGFWIQNDIIWRKTNPMPNFRGKRFTNAHETLIWAARDRKSRVTFNY
                     EAMKAGNDDLQMRSDWLFPICAGPERLKDANGRKAHPTQKPEALLHRILVATTNPGDV
                     VLDPFFGTGTTGAVAKRLGRHWIGIERDPEYAKAAKERIDKVRPVPTSALEMARSKRS
                     EPRVPFGALIELGILEPGSALYDERRRFRAEVRADGTVAWGGEQGSIHRIGAAVQGKA
                     ACNGWTFWHFEAQGQLKPIDTLREMARQRLGLAPSQPIVIAA"
BASE COUNT          506 a          807 c          807 g          515 t
ORIGIN      
        1 ctctcggcgc aaacaccgac ccttatcagc ccatcgagcg ccaataccgc atcacccgct
       61 ccgtgctgga agtgcttgat cgtttcagcc atccggtcag catcgtcacg aaatcagctc
      121 tcgttgtccg cgacatcgac atcctcgcgc gaatggctga gcgcgatctt gtgaaagtgg
      181 ctttgtcggt taccacgctg gatcgccgta tcgcccggaa gatggagccg cgggcgacta
      241 ctccctggcg gcggctcgac gccatcaagc agctcagtgc tgccggagtg ccgacacagg
      301 tgatggtcgc tcccattgtt ccggccatca acgacagcga aatcgagcgc attctcgagg
      361 cggctcatga cgcgggcgct cgtgactccg gctacgttct tcttcggctg ccccttgagc
      421 tcaaggagct gttccgggaa tggctggaga cagagtttcc cgaccgcgca aaacgtgtga
      481 tcagcctcct gcaatccatg cacggcggtc gcgactacac gccagagttc ggcttgcgcc
      541 agcgtggcag cggcccctac gcggagcaga ttgcgatgcg gttccggctt gcccttaagc
      601 ggctcgggat gaacgagcgg gggctgaagc tacggacgga tctcttccaa aagcctgaaa
      661 agagcagtgg agagcagctg caactccttt gacatctcgg cgttgagcct tagaacctcg
      721 gctcatgggg ggaaccgaat cagagccgag gccgacattc gaggtggaag ccgtggcgat
      781 acggctgacc ggcggtccag tcgcgggcat cgacgaggcg gggcgcggcc cccttgcagg
      841 cccggtcgtc gcggcagccg tgatcctcga tcccgacaac atccctccgg gaattgctga
      901 ttccaaggcg ctggagcccg acgttcgaga ggagctgttc gagcgcatca aagccacgtc
      961 gaagatcggt attggcattg cggacgttgc gcgcattgac cgctacaaca ttttgaacgc
     1021 cacgctgtgg gccatgcagc aggcggtgaa gcaactcgag cccacaccgc gtgtggccct
     1081 tgtcgacggc aacaggccgc cgaagcttcg ctgccaaacc cagtgcattg tccaggggga
     1141 cacgaagagt ttgtcgatcg ccgcggcatc catcgtcgcg aaggtcacgc gcgacagaat
     1201 catgatcgag cttggccgcg cttatcccgg ctacggcttc gagcgccaca agggctatcc
     1261 tacaccggag cattttgagg ctatcgatcg cctcggcgtc accccgcacc atcgccgctc
     1321 cttcaggccg gtgcaggttg cgctcgggct cgttccgccc gaagaagcgg agttgccttt
     1381 gctgggcttc aatcaagcct gatcagccct gaactcgcct taaccctttg ttaaccctgc
     1441 atgctgagga tgcccctgcg ggctgagttt ccttgcctgt gttcgtttcg tttgcgagtt
     1501 gcgtcatggt attgcgtcac cgcgtacgcc cgagtcttag cctcgatcag gtcatcgtcg
     1561 gcgactgcct cgaggagctt gcgaagttgc cgacagccag cgtcgatctc gtcttcgccg
     1621 atccccccta caaccttcag ctcgaaaagg atcttctgcg gccaaacaat actcgcgtcg
     1681 acggtgtcga cgatgagtgg gacaagttta ctgatttcga ggagtacgac aggttttccc
     1741 gggcttggct aagcgagtgc cgccgggtgc tgaagcgcga tggcgccatc tgggtcatcg
     1801 gctcctatca caacatcttc cggctcggcg tagctttgca ggacctgggc ttctggatcc
     1861 agaacgacat catctggcgc aagacgaacc cgatgccgaa tttccgtggc aagcggttca
     1921 ccaatgcgca cgagacgttg atctgggctg cgcgtgatcg gaaatcgcgg gtcacgttca
     1981 attacgaggc catgaaggcc ggcaatgatg acctgcagat gcggtcggac tggctgttcc
     2041 cgatttgcgc agggcccgaa cggttgaagg atgccaatgg ccgcaaagct catcccactc
     2101 agaagcccga ggcgctgctg catcgcatcc ttgtggcaac gaccaaccct ggtgatgtcg
     2161 tccttgatcc cttctttggc accggaacta cgggcgcggt agcaaagcgg cttggccgcc
     2221 attggatcgg catcgagcgt gatcctgagt atgcgaaggc agccaaagag cgcatagaca
     2281 aggtgcggcc ggtccccacc tccgcgctcg aaatggcacg ctccaagcga agtgaacccc
     2341 gggtaccctt cggggcgctc atcgagttgg gaattctcga gcccggttca gcactctacg
     2401 acgagcgccg caggttccgg gcagaagtgc gggcggatgg cacagtcgcg tggggtggag
     2461 agcaaggctc gattcaccgc atcggtgccg ccgttcaggg gaaagctgct tgcaacgggt
     2521 ggaccttctg gcacttcgag gcgcaagggc agctgaagcc catcgataca cttcgtgaga
     2581 tggctaggca gcggctcgga ttggctccct cacagccaat cgtgatagcc gctga
//
LOCUS       DASUMG010000228         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_511775, whole genome shotgun sequence.
ACCESSION   DASUMG010000228 DASUMG010000000
VERSION     DASUMG010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511775"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VFX46_03815"
     CDS             <1..634
                     /locus_tag="VFX46_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HEX5599743.1"
                     /translation="PGPGQIVSSNPLGVAALLTQAGADARLLGIARDNRESLIQHVEA
                     ARDADVLVTIGGASVGDHDLVGPVLQSLGMELSFWKIDMRPGKPLLYGRLGPQRVIGL
                     PGNPVSALICTRVFLVPLVRRLLGLPDEEAAAVRAVLAEPVEANGPRQHYMRATLQRE
                     PDGRQLVRLARSQDSSLLAPLAASNVLLVRPPHAPALPAGAEVEVLLLDF"
     gene            756..1463
                     /gene="lexA"
                     /locus_tag="VFX46_03820"
     CDS             756..1463
                     /gene="lexA"
                     /locus_tag="VFX46_03820"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX5599744.1"
                     /translation="MLTQKQKELLLFIHARLQETGVPPSFDEMKDALDLKSKSGIHRL
                     ITALEERGFIRRLPHRARALEVIKLPENAEAAQPRRTGFTPSVIEGRLGKQSQLAIPS
                     SSMIDTRTVSVPVMGRIAAGVPISAIQTHTHDIACPPELLSTGEHFALEVRGDSMIEA
                     GIQDGDIVIIKRCNTAENGDIVVALVDGEEATLKRLRKRGSAIALEAANPNYETRIFG
                     PDQIEIQGRLVALMRRY"
     gene            complement(1595..>2204)
                     /locus_tag="VFX46_03825"
     CDS             complement(1595..>2204)
                     /locus_tag="VFX46_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599745.1"
                     /translation="SRPDILVGRNAELVAVRGPQGRLSALPAAGGSYELQRWLESDGD
                     ARSYHPASDVFRCDAVGCTANVKNRTVAVVRHPAALAEDCSRADILVIPFPRPKGCNP
                     KEIAIDFYAVRDRGTHAVYIGETGISVVTVVDFRGNRPWSRSPERPRRSGRAERQNAA
                     SQTAESIASDDLSETSRRSHSNFEDDDDPVIDQLQAQSEEEP"
BASE COUNT          394 a          705 c          689 g          416 t
ORIGIN      
        1 gcccggccca ggtcagatcg tctcgtccaa tccactggga gtggcggcgc tcctcacaca
       61 agccggcgca gatgcgagac ttcttggcat cgcgcgggac aatcgcgaga gccttatcca
      121 gcatgtcgaa gcggcccggg atgccgatgt tctggtgacc atcggaggcg cctccgtggg
      181 agaccacgac ctcgtcgggc cggtgctgca aagcctcggc atggagctgt ccttctggaa
      241 gatcgacatg cggccgggca agccgctgct ctacggccgt ttggggccac agcgcgtcat
      301 cggcttgcca ggcaatcccg tatccgcact gatctgcacg cgggtattcc tcgtgcccct
      361 ggtgcggcgc ctgcttggcc tgcccgacga ggaggcggcc gccgtccgtg ctgtccttgc
      421 cgagcctgtg gaagccaacg gccctcggca gcactacatg cgggctacac tgcaacgcga
      481 gcccgacggg cgccagctcg tgcggcttgc acgctcccag gacagctcgc tcttggcccc
      541 gctcgctgcc agcaacgttc tgttagtacg gccgccgcat gctccggcgc tgcctgccgg
      601 tgccgaggtc gaagttctcc ttctggattt ttagccctcg gccgtccttt gtggacgggc
      661 aggcttgcag aacaagaccg gtacgagtat cttgttcatg ttttgtacag gcacacgggt
      721 agcagggctg tcttgcagat ggggcgccgg atcagatgct gacacagaaa cagaaagagc
      781 ttttgctttt cattcacgcg cgcctgcagg agaccggtgt tccgccgtcg ttcgatgaga
      841 tgaaggatgc tctggacttg aagtcgaagt ccggcatcca tcggctcatc accgccctcg
      901 aggagcgcgg cttcatccgg cgtttgcccc accgcgctcg tgcgctcgag gtgatcaagt
      961 tgccggagaa tgcggaggca gcccagccac ggcgaaccgg cttcacgccc agcgttatcg
     1021 agggccggct cggcaagcag tcgcagctcg ccatcccatc atccagcatg atcgacaccc
     1081 gcacggtctc cgtgccggtc atgggccgca tcgctgccgg cgtgccgatt agcgccattc
     1141 agacccacac gcacgacatc gcgtgtcctc cggagctgtt gtccactggc gagcactttg
     1201 cgttggaggt gcgcggcgat tcgatgatcg aagccggcat ccaggacggc gacatcgtca
     1261 tcatcaagcg ctgcaatacg gcggagaacg gcgatatcgt cgtcgcttta gtggatgggg
     1321 aggaggcgac gctcaaacgt ctccggaagc gcggctccgc catcgctctc gaagctgcca
     1381 acccgaatta cgagacgcgc atctttgggc cggaccagat cgagatccag gggcgtctcg
     1441 tcgccctgat gcggcgctat tgaggctgca gtagccggac attggtgcat cacagtcgtg
     1501 tcgttgccgg cgcggaccac gaacagcgcg caaggtttga gatcgtgagc tgacgctctg
     1561 ccagcaccgc tccctcgcat tcgaggatag ctgcttaggg ctcctcctcg gactgagctt
     1621 gcagctgatc gatcactgga tcatcgtcat cctcgaagtt ggaatggctt cggcgtgacg
     1681 tttcgctcaa gtcgtccgag gcaatgctct cagctgtctg agaggcggcg ttttggcgct
     1741 ccgcacgccc gcttcggcgc ggccgctcag gtgacctcga ccatgggcgg ttgccgcgga
     1801 aatcgacgac ggtcacaacg gaaatgcccg tttccccgat gtagacggcg tgggttccgc
     1861 gatcgcgaac ggcataaaaa tcgattgcga tttccttcgg attacagccc ttgggccgag
     1921 gaaatggaat tacgagtata tcagcgcggc tacaatcttc agccaaagcc gcgggatggc
     1981 gcaccaccgc aaccgtccga ttcttgacgt tcgccgtgca gccgacggca tcgcagcgaa
     2041 acacatccga ggccgggtgg taagatcgcg cgtcaccatc gctttcgagc cagcgctgca
     2101 gctcatagct ccctccagcg gcgggcagcg cggaaagccg gccttgcggc ccacgaacgg
     2161 cgacaagctc cgcattgcga ccgacgagaa tgtcaggccg cgac
//
LOCUS       DASUMG010000229         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_350916, whole genome shotgun sequence.
ACCESSION   DASUMG010000229 DASUMG010000000
VERSION     DASUMG010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350916"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="frc"
                     /locus_tag="VFX46_03830"
     CDS             complement(<1..1046)
                     /gene="frc"
                     /locus_tag="VFX46_03830"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX5599746.1"
                     /translation="MKALEGVRVLDFTHVQSGPTCTQLLAWFGADVIKVERPGVGDIT
                     RTQLRDIPDVDSLYFTMLNHNKRSITLDTKHPKGKEVLERLIKACDVLVENFAPGVLD
                     RMGFSWERIQELNPRMIYASIKGFGPGPYEDCKVYENIAQCTGGAASTTGFDDGPPLV
                     TGAQIGDSGTGLHLALGIVTALYQRTRTGKGQRVTCAMQDGVLNLCRVKLRDQQRLTR
                     GPLTEYPQYSEGKPFGDTVPRSGNASGGGQPGWILKCKGWETDPNAYIYFIAQAPVWD
                     KICDVIGEPDWKTHPDYATPQARLPRLKQIFDRIEQWTMTKTKFEAMEILNKYDIPCG
                     PILSMKELAEDQSLR"
     gene            complement(1057..>2124)
                     /gene="oxc"
                     /locus_tag="VFX46_03835"
     CDS             complement(1057..>2124)
                     /gene="oxc"
                     /locus_tag="VFX46_03835"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HEX5599747.1"
                     /translation="RPLIILGKGAAYAQADDAIRALVEKSGIPFLPMSMAKGILPDTH
                     PQCAGAARSLVLKESDVVMLIGARLNWLLSHGKGKTWGDAPKKFIQIDIEPKEIDSNV
                     GIAAPIVGDIGSCVSRLIEEMGNNWPAPPADWIKTVNAKKEENIAKMAPRLMNNSVPM
                     DFHAALGALRTVIRERPDTILVNEGANTLDLARGVIDIYKPRKRLDVGTWGVMGVGTG
                     FAIAAAIETGHPVLAVVGDSAFGFSGMEVETICRYNLPVCIVVFNNNGIYRGVEVNPS
                     GGPDPAITVFVKDARYDKMMEAFGGVGVYATTPDELKRAVDAALDSGKPTLVNAVIDP
                     AAGSESGRIGNLNPQSVLKKK"
BASE COUNT          398 a          650 c          631 g          445 t
ORIGIN      
        1 cgcagcgact gatcctccgc caactccttc atcgacagga tgggcccgca ggggatgtcg
       61 tatttgttga ggatctccat ggcctcgaac ttcgtcttgg tcatggtcca ctgctcaatg
      121 cgatcgaaga tctgcttgag ccgcgggagg cgtgcctgag gcgtcgcata gtcggggtgc
      181 gtcttccaat caggctcgcc gatcacgtcg caaatcttgt cccaaacggg cgcctgagcg
      241 atgaagtaga tgtacgcgtt cggatcggtc tcccagcctt tgcacttcaa aatccagccg
      301 ggctgaccac cgccggaggc attgcctgag cgcggaacgg tgtcgccgaa cggcttaccc
      361 tcgctgtatt gcggatactc agtcagcgga ccgcgggtga gacgctgctg atcacgcaac
      421 ttcacgcggc agagattgag cacgccgtcc tgcatggcgc aggtgacgcg ctgacccttg
      481 ccggtgcgcg tccgttgata aagagccgtt acaatgccaa gggccaagtg caatccggtg
      541 ccgctatcgc cgatttgcgc gccggtgacg agcggaggac cgtcatcgaa accggttgtg
      601 gaggcggcgc cacccgtaca ctgggcgatg ttttcgtaga ccttgcaatc ctcataaggg
      661 ccaggaccga accctttgat ggaggcataa atcatccgcg gattgagctc ctggattcgc
      721 tcccaggaaa aacccatccg gtcgagcact cccggcgcaa agttctccac cagcacgtcg
      781 caggccttga tcagccgctc cagcacctcc ttgcctttcg ggtgcttcgt atcgagcgtg
      841 atcgaccgct tattgtggtt cagcatcgta aagtagaggc tgtccacatc cggaatatcc
      901 cgcagctgag tacgggtgat atcaccgacc cccggccgct cgaccttgat gacgtcagca
      961 ccgaaccaag cgagaagctg cgtgcaggtg ggaccggact gcacgtgcgt gaaatcgaga
     1021 acacggacgc cttccagggc tttcatatca agctccctat ttctttttca gcacgctttg
     1081 cggattgagg ttgccaatac ggccgctttc gctgccggcc gccgggtcga tgactgcgtt
     1141 gacgagcgtc ggcttgcccg aatccagagc cgcatctacc gcacgcttca gctcatcggg
     1201 cgttgttgcg tagactccaa cgccgccgaa ggcctccatc attttgtcgt agcgggcgtc
     1261 ttttacgaaa acagtgatag ccggatcggg accgccggac ggattgacct caaccccgcg
     1321 ataaatgccg ttgttgttga agacgacgat gcagacgggg agattgtacc ggcagatcgt
     1381 ctcgacctcc attccggaga agccgaaggc gctgtcgccg acaaccgcga gcacgggatg
     1441 gcccgtctcg atcgcggctg cgatggcaaa gcctgttccg acgcccatga cgccccaggt
     1501 gccaacgtcg aggcgcttcc gaggcttgta gatgtcgatg acgccccgcg ccaaatccag
     1561 cgtatttgcg ccttcgttga cgagaatagt atcgggccgc tccctgataa cggtccgcaa
     1621 ggcgccgagc gccgcatgga aatccatcgg caccgagttg ttcatcagcc gcggcgccat
     1681 cttggcgatg ttttcttcct tcttggcgtt gactgttttg atccaatccg ctgggggagc
     1741 cggccagttg ttccccattt cttcaattag ccgggacacg caggagccga tatcgcccac
     1801 gatcggcgcg gcgatcccca cgttgctgtc gatttccttg ggctcgatgt cgatttgaat
     1861 gaatttcttc ggcgcgtcgc cccaggtctt gcccttgccg tgcgagagca gccagttgag
     1921 ccgcgcgccg atgagcatta cgacatcgga ctctttcagc accagcgagc gcgcagcgcc
     1981 ggcgcattgc gggtgcgtgt caggcagaat gcctttcgcc atgctcatgg gcaggaacgg
     2041 tatgccgctc ttttcgacga gtgcgcggat cgcatcgtcg gcctgggcat aggccgcgcc
     2101 tttgccgaga atgatgagcg gacg
//
LOCUS       DASUMG010000230         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_190389, whole genome shotgun sequence.
ACCESSION   DASUMG010000230 DASUMG010000000
VERSION     DASUMG010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190389"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFX46_03840"
     CDS             complement(<1..613)
                     /locus_tag="VFX46_03840"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599748.1"
                     /translation="MSETFGFIGLGNMGHPMAGRLIDAGHSLLVHDVRASVVDQFVER
                     GAKRASSPAEVASQVETIFLSLPTPPIVETVALGENGLASGDKVKRIIDLSTTGPQTA
                     EKIANAFKARSVRWLDCPVSGGVGGAKAGTVAVMFSGPREDYDDLFPVLQVIGKPFYI
                     GDKPGLAQMMKLVNNCLSAAAMALTSEAAVVGAKAGIPPDVMID"
     gene            1269..1754
                     /locus_tag="VFX46_03845"
     CDS             1269..1754
                     /locus_tag="VFX46_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX5599749.1"
                     /translation="MVEPVELTTILGDEDLLLDSPIESKDTLLAAAADHLGRTTGVPS
                     EVILKALSDREALGSTAINHGVAVPHAGMDDLPRPAAVFFRLAHPIEFDAPDNNPVDL
                     VFVAIWPSDQRSGLLATLGSLCRNLREPPVRQALRTASSPKEVRALLGRGAVPDANKS
                     S"
     gene            1944..>2230
                     /locus_tag="VFX46_03850"
     CDS             1944..>2230
                     /locus_tag="VFX46_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599750.1"
                     /translation="MSTDAILLTVLVVMPFFGSIVAALLPTNAHNLEAWWAAGIAFIG
                     LAIAGTFSGPMAEGVVIRAKFEWVPQLGLNFVLRLDGFAWSFVVLVCLIGLL"
BASE COUNT          408 a          702 c          673 g          447 t
ORIGIN      
        1 cgtcgatcat cacgtcgggt ggaatcccgg ccttcgcgcc tacaacggcg gcctccgacg
       61 ttaacgccat cgctgcggcg gacaggcagt tgttgacgag cttcatcatc tgggccaggc
      121 ccggcttgtc accgatgtag aacggcttgc cgatcacctg cagcaccggg aagaggtcgt
      181 cgtagtcctc acgcggcccc gaaaacatca cggctacggt tccggccttg gcgccgccca
      241 caccgccgct taccgggcaa tcaagccagc ggacgctgcg cgccttgaaa gcgttggcga
      301 ttttctctgc ggtctggggg cccgtcgtgg agaggtcgat gatccgcttg accttgtcgc
      361 ccgaagccag accattctcc ccgagggcca ctgtctcgac gatcggtggc gtcggcagcg
      421 acaggaaaat ggtctccact tgggaggcga cttccgctgg cgatgaggcc cgcttcgcgc
      481 cccgctcaac gaactgatcg acgacgctgg cgcgcacgtc gtgaacgagt agagaatggc
      541 cggcgtcgat caaacgcccg gccatagggt gccccatgtt gccgaggccg atgaatccaa
      601 aggtctcgct caatgcatcc tcccgattgc gtgcgccgac cccgcgctcc aagccaaggg
      661 cgaagctgac aatggtcggc gctgatctgc cctgcccatc tcgaataagt cgaggcggga
      721 gcatccagac ttgtctagcc gaccaccgtg cctgcggcgc tgcctagtac aagtccagat
      781 tatagatgct gttacggtaa tacaatcgac agattggcga cggagccatt cgatatctgc
      841 tgtcaacacc ggcgccgctc cttcctcgta ctgcctgtga cggggctcaa acacgctcag
      901 aggcgagact gcgacgaatt tcgcatatcg atctgctcag ggttcggcta accccttccc
      961 gctgttggtg atgcccacgt ggcactcctc acggctggga agatgatcac ctcttgcggc
     1021 gtgcgcttct ccgcactaaa gggtgctcca cccaagatca tcctggaggg accggaacgt
     1081 cgtgatctac cagggaatgg ttgtcgttca atcgattggc ctcaggatgg ctggtacgca
     1141 cgttgggttc tgattggtag cgatgccaat agcacgtgcc agcgaatgcc ctgcgacatg
     1201 gactgccctc aatctccgct gtccgagccc cactccgggg ccgtgatacc gctctcgcca
     1261 ggaggcgcat ggtggaaccc gtagaactca cgacgattct cggcgacgag gacctgcttc
     1321 tggactcgcc tatcgagtcg aaggacacac tcctcgcggc agccgcggat cacctcggac
     1381 ggaccaccgg cgtcccaagc gaggtcatcc tgaaagcctt gtcagaccgc gaggcgctgg
     1441 gctcgacggc aatcaatcac ggagttgcgg tgccccacgc ggggatggat gacttgccgc
     1501 ggccggcagc ggtcttcttc cggcttgcac atccgatcga gttcgatgcg ccggacaaca
     1561 accctgtgga tctggtgttc gtcgcgatct ggcccagcga ccagaggagc gggctgctgg
     1621 caacattggg cagcttgtgc cgcaatcttc gcgagccgcc tgtgcgacag gcgttgcgga
     1681 ctgcttcaag tccgaaggaa gttcgagcgc ttctcggccg gggcgcagtt ccagacgcca
     1741 ataagtcctc ttgataggtg cgtgacggcg tttgccgtag ccgcgaccat cccgtaaata
     1801 tgaaattgcg tgtggcgcgc ggggctaatc cctcaacgag ccttgccgcg ccgcggcttg
     1861 gcgccgtctt gttgctgtcg gcagacagac cctaaagtcg gcgcgacctg ccatcctcac
     1921 gaacgaaagg ttgctcgccg agcatgtcga ccgacgccat cctcctgact gtcctggtcg
     1981 taatgccatt tttcggcagc atcgtcgcag cactcctgcc aaccaatgca cataacctcg
     2041 aggcatggtg ggcagcgggg atagcgttca ttggcctagc cattgccggc actttctccg
     2101 ggccgatggc agaaggcgtg gtcattcgcg ccaagttcga gtgggtaccc caattagggc
     2161 tgaattttgt tctgcggctc gatggcttcg catggagctt cgttgtcctt gtatgcctga
     2221 tcgggctgct
//
LOCUS       DASUMG010000231         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_672492, whole genome shotgun sequence.
ACCESSION   DASUMG010000231 DASUMG010000000
VERSION     DASUMG010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672492"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1367
                     /locus_tag="VFX46_03855"
     CDS             117..1367
                     /locus_tag="VFX46_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX5599751.1"
                     /translation="MKYSIFSLLRQAANAHQGWPRQWRDPEPKPAYDVVIIGAGGHGL
                     ATAYYLAKEHGIRNVAVLEKGWLGGGNTGRNTTIIRSNYLWEESEAIYEHAMKLWETL
                     SDELNYNVMYSPRGVMMLAHNVHDVQSFKRHVHANRLAGIDNEWLTPEEAKAFCPPLN
                     ISPNLRYPVLGAALQRRGGVARHDAVAWGYARAADKLGVDIIQNCEVIGIRRGPDGAV
                     EAVETTRGTIKARKVGIVAAGHTSVIAAMAGFRLPLESFPLQALVSEPVKPVFPCVVM
                     SNTMHAYISQSDKGELVIGAGTDQYVSYSQTGGLHITTHTLDAICELFPMFRRMRMLR
                     NWGGIVDVTPDRSPIIGKTPVPNLFINCGWGTGGFKATPGSGHVFAATIARGEPHPIA
                     APFTLDRFRTGRLIDEAAAAAVAH"
     gene            1377..1688
                     /locus_tag="VFX46_03860"
     CDS             1377..1688
                     /locus_tag="VFX46_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX5599752.1"
                     /translation="MLLIPCPCCGEARPEIEFRYGGEAHIARPRDPSALNDEQWADFL
                     YMRTNPKGIHFERWRHVHGCGRFFNAVRDTVSDRILLTYKSGEPRPDAATIEAAKEGR
                     R"
     gene            1688..>3797
                     /locus_tag="VFX46_03865"
     CDS             1688..>3797
                     /locus_tag="VFX46_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5599753.1"
                     /translation="MNPAFRTTTGGRIDRSHPIRFTFDGKSYTGYKGDTLASALLANG
                     VHLMGRSFKYHRPRGPIAAGAEEPNALVGVIGPNGRETPNLRATQVEIYDGLVAESQN
                     RWPSLSFDANAVNDLFSPLLPAGFYYKTFMGPRFLGATRVWKSIYEPLIRRAAGLGRA
                     PRSPDPDRYANRFAHCDVLIIGGGPAGLSAALAASEAGADVILCDEQAELGGSLLSDT
                     RSRINGQTAAEWLAQTLATLSSRPNVRLLPRTQAFGYYADNFVALQERVTDHLANPSP
                     NLPRERLWQVRAKEVVIATGAIERPLVFPENDRPGIMLADAARTYVNRYGVRPGNQAV
                     VFTACDTAYEAALALHEAGVEVRLIADLRPNPSGPAVDAARRAGIKVMGSATILGTHG
                     RLRISSAHVGLVDGLGKVVPYEHVACDLLLMSGGWTPSVHLYSQSRGKVVWNEEAQAF
                     LPGEHVQRERSAGACRGVFGLNAALEDGASAGRDAAEKAGHQPPPAVTFTVEELQHSY
                     RGVLEACPHHRDPARVKAFVDFQNDVTAKDLKIATREGFRSIEHVKRFTTTGMATDQG
                     KTSNVNALSIVSDTLRQPIPTVGLTTFRPPYTPVTFGVLAGERRGDLFDPVRTTPIHS
                     WAVEHGAVFEDVGLWKRARYFPRPGEDMHAAVSRECRTVRASVGLLDASTLGKIEVVG
                     PDAAEFLERMYVNPWKNLQPG"
BASE COUNT          659 a         1205 c         1234 g          699 t
ORIGIN      
        1 gtatcttagt gaggacaagt cgacgcagcc cgtgtggtca catgagagcc gtgttgctcg
       61 tttgctgatg cagggtaatc aattagcgtc tgtgatcatg agcggggtag aatccgttga
      121 agtactcgat ctttagtctt ctccgtcagg cagccaatgc ccatcagggt tggccgcggc
      181 agtggcgcga tcctgagccc aaaccggcct atgatgtcgt gatcatcggt gctggtgggc
      241 atgggctcgc gacagcctat tacctcgcca aggaacacgg cattcgtaac gtcgctgtcc
      301 tcgagaaagg ctggctcggc ggcggcaata cggggcgcaa cacgacgatc atccgctcga
      361 attacctctg ggaagagagc gaggcgatct acgagcacgc gatgaagctc tgggagaccc
      421 tctccgacga gctgaactat aacgtcatgt actccccgcg cggtgtgatg atgctggcgc
      481 acaatgtgca tgacgtgcag agcttcaagc gccacgttca tgccaatcgg cttgcgggca
      541 tcgacaacga atggctgaca ccggaggagg cgaaggcctt ctgtccgccg ctcaatatct
      601 cacccaatct ccgctacccg gttctaggtg cggccctaca gcgtcggggc ggcgtggcgc
      661 ggcacgatgc ggtggcctgg ggctacgccc gcgccgcaga caagctcggc gtcgacatca
      721 tccagaactg cgaggtgata ggcattcgcc gcggccccga tggggcggtc gaggcggtcg
      781 agaccacgcg ggggacgatc aaggcccgca aggtcggcat cgtggctgcg gggcacacca
      841 gcgtgatcgc ggccatggcc ggcttccgtc tgccgctcga aagcttcccg ttgcaggcac
      901 tggtgtcgga gccggttaag ccagtgttcc cgtgtgtggt gatgtcgaac accatgcacg
      961 cctacatctc ccaatccgac aagggcgaac tcgttattgg cgccggcacg gaccagtacg
     1021 tctcctacag ccagaccggc ggcctgcaca ttaccacgca cacgcttgat gcgatttgcg
     1081 agttgttccc gatgttccgg cgcatgcgga tgctgcgcaa ttggggcggc attgtcgacg
     1141 tcacgccgga ccgctcgccg atcatcggca agacgcccgt gccgaacctg ttcatcaact
     1201 gcggctgggg aacgggcggg ttcaaggcaa cgcccgggtc cggccacgtg ttcgcggcca
     1261 cgatcgctcg cggcgagccg cacccgatcg ccgctccctt cacgctcgac cgcttccgca
     1321 ccggccggtt gatcgacgag gccgcggctg cggccgttgc gcactgaggt tctccaatgc
     1381 tgctgatccc ttgtccctgt tgcggtgaag cgcggcccga aattgagttc cgctacggcg
     1441 gtgaggcgca tatcgcgcgc ccccgcgatc cgtctgcgct gaacgatgag cagtgggccg
     1501 attttctcta catgcggacc aatccgaagg gcatccactt cgagcgttgg cggcatgtgc
     1561 acggctgtgg gcgtttcttc aacgcggtgc gcgacaccgt cagtgatcgt attctgttga
     1621 cctataagtc cggcgagccg cgacctgacg cggccacaat tgaggcggcg aaggaggggc
     1681 gccgatgatc aatccggcgt tccggacaac aacgggtggc cgcatcgacc gctcgcaccc
     1741 gatccgcttc accttcgatg gcaagagcta cacaggctac aaaggcgata cgctggcctc
     1801 ggcgctgctc gccaacggcg tccacctcat ggggcgttcg ttcaagtatc atcgtccccg
     1861 cgggccgatt gcggcggggg cggaggagcc gaatgcgctc gtcggtgtaa ttggcccgaa
     1921 cggccgcgag acgccgaacc tgcgagcgac tcaggtcgag atctacgatg ggctggtagc
     1981 cgaaagtcag aaccgttggc cgtcgctgtc cttcgacgca aatgccgtca atgatctttt
     2041 ctctcccctt ctgcctgcgg gcttctacta caaaaccttc atgggaccgc gcttcctcgg
     2101 cgcgacgagg gtctggaagt cgatctacga accgctgatc cgccgggctg caggcctggg
     2161 acgagcacct cggtcgcccg atccggatcg gtacgcgaac cggttcgcac attgcgatgt
     2221 gctcattatc ggtggcggac cagcgggttt gtcggcggcg cttgcggctt cggaagcggg
     2281 tgcagatgtg atcctctgcg acgagcaggc ggagctcggc ggctcactgc tgtccgacac
     2341 ccgctcgcgc atcaatggtc agacggccgc cgaatggctg gcgcagacgc tcgcaacgct
     2401 gtcgagccgc cccaacgtgc ggctgctgcc gcgaacgcag gcgttcggat actacgcgga
     2461 caacttcgtc gccctgcagg agcgggtgac ggatcatctc gccaacccgt cgccgaacct
     2521 gccacgcgaa cggctctggc aggtgcgggc caaggaagtc gtgattgcca ccggcgccat
     2581 cgagcggccg ttggtattcc cggaaaacga ccggccgggc atcatgttgg ccgacgccgc
     2641 gcggacctat gtgaaccgtt acggtgtgcg gcccggcaac caagccgtcg tgtttacggc
     2701 gtgcgatacg gcttacgaag ccgcgctcgc attgcacgag gcaggcgttg aagtgcgcct
     2761 gatcgccgat ttgcggccta acccaagtgg tcccgctgtc gatgcagcgc gccgtgccgg
     2821 gatcaaggtt atgggctcag ccaccattct cggcacgcac gggcggctgc gcatttcgtc
     2881 cgctcacgtt gggctcgtcg atggccttgg caaggtcgtg ccgtacgagc acgtcgcgtg
     2941 cgatctgctg ctgatgtccg gcggctggac accgtcagtg cacctttatt cgcaatcgcg
     3001 cggcaaggtc gtctggaacg aggaggcgca ggccttcctg ccgggcgagc atgtccagcg
     3061 ggagcgctct gccggagcgt gccgcggcgt gttcgggctc aacgccgctc tggaggatgg
     3121 tgcttctgcc ggccgggatg ccgcggagaa agccgggcat caaccaccgc ctgcggtgac
     3181 gttcaccgtc gaagagctgc agcactccta tcggggcgtg ctcgaggcat gtccgcatca
     3241 tcgcgatccg gcgcgggtca aggcgtttgt cgatttccag aacgacgtca cggccaagga
     3301 tctcaaaatc gccacgcgag aaggcttccg ctccatcgag catgtgaagc gcttcaccac
     3361 gacgggcatg gccaccgacc agggcaagac gtcgaatgtg aacgccttgt ccattgtctc
     3421 cgacacactc aggcagccaa tcccgacggt gggtctgacg acgttccgtc caccgtatac
     3481 gccggtaacc ttcggcgtcc tcgcgggcga gcgccgcggt gatctgttcg atccagtccg
     3541 caccacgccc attcattcct gggctgtcga gcatggcgcg gtgttcgagg acgtcggtct
     3601 ttggaaacgc gcgcgttatt tcccgcgccc gggcgaggac atgcacgccg ccgtctcgcg
     3661 cgagtgccgg accgtgcgcg ccagcgtcgg gctgctcgat gcctcgacgc tcggcaagat
     3721 cgaggtcgtg gggccggacg cagccgagtt cctcgagcgc atgtacgtca atccgtggaa
     3781 gaacctgcag ccggggc
//
LOCUS       DASUMG010000232         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_29441, whole genome shotgun sequence.
ACCESSION   DASUMG010000232 DASUMG010000000
VERSION     DASUMG010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29441"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /gene="hisS"
                     /locus_tag="VFX46_03870"
     CDS             <1..1486
                     /gene="hisS"
                     /locus_tag="VFX46_03870"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX5599754.1"
                     /translation="KARLPRGFVDRPAEDIRAVEKMMAKIREVYELYGFEPVEQPLIE
                     YTDALGKFLPDQDRPNDGVFSFQDEDEQWLSLRYDLTAPLARHFAENFQFLAQPYRSY
                     RFGWVFRNEKPGPGRFRQFMQFDADTVGSASPAADAEICMMAADTMEALGIPRGSYVV
                     KVNNRKVLDGVLEAIGLGGDANAGRRLTVLRAIDKLDRLGTEGVKALLGPGRKDESGD
                     FTKGAGLDDPAIDRVLAAISVQAGDTDVVLTKLNLAASRLGEEGISELRSIAELVRAG
                     GYDEGRIKIDPSVVRGLEYYTGPVYEVELTFPIEGEDGKSVRFGSVAGGGRYDGLVAR
                     FRGEAVPATGFSIGVSRLTAALQHLGKIDTKPEPGPVVVTVFPDVPIAEYQRLVARLR
                     DAEYAPGKKIRAELYLGSGKFGAQMKYADKRGSPCVVIQGGNEKEKGEVQIKDLILGA
                     EIAGLSKDRDDYLKKQAEAQFAVRESELVDAVKKVLARNNVTWG"
     gene            complement(1759..2310)
                     /locus_tag="VFX46_03875"
     CDS             complement(1759..2310)
                     /locus_tag="VFX46_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEX5599755.1"
                     /translation="MRRWLPSVAFALVWAAPAAAIDMPERKAGLWELKMTFEGGSLPP
                     QTVEHCIDAETDKLMSATGGSMVQDMCSKPDVQKAGDTITVDSTCKIGPYATSSHSVI
                     TGDFNSAYSVKVVSKQQGPAIPGMPAERTMTVEGKWMGACKADQKPGDMIMAGRKINV
                     RDLQNMPGPPGAPARPGAPAQKK"
BASE COUNT          461 a          759 c          851 g          494 t
ORIGIN      
        1 caaagcgcgg ctgccgcgcg gctttgtcga ccgtccggcc gaggatatcc gcgccgtcga
       61 gaagatgatg gcgaagatca gggaggtcta tgagctctac ggcttcgagc cggtcgagca
      121 gccgctgatc gaatacaccg acgcgctcgg caagttcctg cccgaccagg accggcccaa
      181 cgacggcgtg ttctcgttcc aggacgagga cgagcagtgg ctgtcgctgc gctacgacct
      241 caccgcgccg ctggcgcggc atttcgcgga gaacttccag tttctcgcgc agccctatcg
      301 ttcgtatcgc ttcggctggg tgttccgcaa cgagaagccc ggccccggcc gcttccgcca
      361 gttcatgcag ttcgacgccg acaccgtcgg ctcggcctcg cccgccgccg acgccgagat
      421 ctgcatgatg gcggcggaca cgatggaggc gctgggcatt ccgcgcggca gctacgtggt
      481 gaaggtgaat aatcgcaagg tcctcgacgg agtgctggaa gcaatcgggc tcggcggcga
      541 cgcgaatgca ggaaggcgac tcaccgtgtt gcgcgcgatc gacaagttgg atcgccttgg
      601 cactgaaggc gtaaaggcac ttctaggccc ggggcgaaaa gacgagagcg gggatttcac
      661 gaaaggagcg gggcttgacg atcccgcgat tgatcgcgtg cttgccgcga tctccgttca
      721 agcgggcgat actgatgtcg tgttgacgaa gctcaacctc gctgcgtcgc gtctcggtga
      781 agagggaatt tccgagttgc gtagcatcgc cgagttggtt cgtgcgggag ggtacgacga
      841 gggtcgcatc aaaatagatc cctccgtcgt gcgcggcctc gaatattaca ccgggcccgt
      901 ttacgaggtg gagctgacct tccccattga gggcgaggat ggaaaatccg ttcgtttcgg
      961 ctcggtcgcg ggcggcggtc gttacgatgg cctggtggcc cgctttcgcg gcgaggcggt
     1021 gccagcgacg ggtttctcca ttggtgtgtc gcgcctgacc gcggcgctcc agcatctggg
     1081 caagatcgac accaagcccg agccggggcc ggttgtcgtg accgtgtttc cggacgtacc
     1141 gatcgcggaa tatcaacgcc tggtcgcgcg gctacgcgat gccgagtacg caccgggaaa
     1201 gaagattcgc gccgagcttt atctcggctc cggcaagttt ggcgcgcaga tgaaatacgc
     1261 cgacaagcgc ggctcgccct gcgtcgtgat ccagggcggc aacgagaaag agaagggcga
     1321 ggtccagatc aaggatctca tcctcggcgc cgagatcgcc gggctgagca aggatcgtga
     1381 cgactatctg aagaagcagg ccgaggcgca gttcgcggtc agggaaagcg agctggtcga
     1441 cgcggtcaag aaagtgctcg cgcgaaacaa cgtgacctgg ggctgatttc gcaagttagg
     1501 gtctgtgcgc aaccattgtc gcgcaaaccg gcggacagac cgtcattcgt agctggcggt
     1561 aggcggtagg ttgggtcgag ttcttcacga gacccaacaa tccctgagca tcgctagatg
     1621 ttgggtctcg cttcgctcga cccaacctac gacttggagc ggggggccgt cgcccttcga
     1681 ggctcgccca ggagggctcg cacctcaggg tgacggatca cctccgctac ctcgtcttgg
     1741 ggcgagcgat cgtctgcact acttcttctg cgccggcgcg cccggccttg caggtgcacc
     1801 cggaggtccc ggcatgttct gcagatcgcg gacgttgatc ttgcggccag ccatgatcat
     1861 gtcgccgggt ttctggtcgg ctttgcaggc gcccatccac ttcccctcga ccgtcatggt
     1921 ccgctccgcg ggcatcccgg gaattgccgg cccctgctgt ttcgacacga ccttgaccga
     1981 ataggcgctg ttgaaatcgc cggtgatgac agaatgggag ctggtggcgt agggcccgat
     2041 cttgcacgtg gaatcgacgg taatggtgtc gccggctttc tgcacgtccg gcttcgagca
     2101 catgtcctgg accatgctgc cgccggtcgc gctcatcagc ttgtcggtct cggcgtcgat
     2161 gcaatgctcg acggtctggg gcggcaggct gccgccttcg aacgtcatct tgagctccca
     2221 gagcccagcc ttgcgctctg gcatgtcgat ggccgcggcg ggcgcggccc agacgagagc
     2281 aaacgcaacg gatggcagcc atcggcgcat cgtaatctcc ttcagcgcga gagcgatgaa
     2341 ttcaccggca ggttctgctc ggcactgtgg gcaaataaag gcggtcgcgg gtgtcccgga
     2401 ttccggagtt cgcgggattg agcgcgtcct cctttgaggg ctccggaatc gaaggcgagc
     2461 gactacaata cgaatttatt tcttgactac gtaacgaata tctagtataa gattgtcatc
     2521 ctcgctcgtg aggggcgtct tctagagacg atcctgaagg cggag
//
LOCUS       DASUMG010000233         2918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_512051, whole genome shotgun sequence.
ACCESSION   DASUMG010000233 DASUMG010000000
VERSION     DASUMG010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512051"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFX46_03880"
     CDS             complement(<1..332)
                     /locus_tag="VFX46_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5599756.1"
                     /translation="MFDQTLRGRIIEAAMKLANERPWKDMTLLDIAEATGVTLVDLKK
                     EFSSKSEILAAFTRAVDDEVLRRAPKRESTTSRRDALFEILMTRFDVLAPYKQALRSI
                     AGDLRAPDP"
     gene            complement(396..1220)
                     /gene="proC"
                     /locus_tag="VFX46_03885"
     CDS             complement(396..1220)
                     /gene="proC"
                     /locus_tag="VFX46_03885"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX5599757.1"
                     /translation="MSLSLKGKLLLAGAGKMGGAMLTGWLDRGLAPSQIIVQDPAPPP
                     ENAELISRHGIEMRQPGAPLPEPPAVILVAVKPQIMDQVFPELATQAGPETLVLSIAA
                     GKTIASFEAHLPAGSAVVRSIPNTPAAIGRGITVCCANAHVSTSQREMCQSLLSAIGE
                     VGWVEDEALIDAATAVSGSGPAYVFLLAECLAEAGRAVGLDADLAKRLANATVSGAGE
                     LLRQSGLEPSVLRQNVTSPGGTTAAALEVLMAKDGLQPLLVRAVEAAARRSRELSG"
     gene            complement(1394..1891)
                     /locus_tag="VFX46_03890"
     CDS             complement(1394..1891)
                     /locus_tag="VFX46_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014490159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX5599758.1"
                     /translation="MASAEAGYQRMSNPIDIVEQVAHTHGWACDRTADDEVTLIVAGS
                     WTDYHLSLNWRDDLEVLHLACAFDFKVPDNRLPEIYKLIAQINEQLWLGHFDFWTQEG
                     LIMFRHGLMLNGALATPPQVEALLKAALEACERYYQAFQFVVWAGKESREALVSTMFH
                     TEGQA"
     gene            complement(2216..2482)
                     /locus_tag="VFX46_03895"
     CDS             complement(2216..2482)
                     /locus_tag="VFX46_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEX5599759.1"
                     /translation="MTQTTNRFFDELAKMMTDAAGAAQGLRREFESFMKAQGERVLRE
                     MDVVRRDEFEAVKAMAQRAREENEKLEARIAALEAELAALKSRV"
     gene            2667..>2918
                     /locus_tag="VFX46_03900"
     CDS             2667..>2918
                     /locus_tag="VFX46_03900"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX5599760.1"
                     /translation="MNSFASFLAIPFPNIDPVALQLGPVTVKWYGLAYMAGLLLGWLY
                     IRRLMRNPALWSGGKPPFGLEKTDDLLFFMTLGVVLGGRL"
BASE COUNT          557 a          943 c          855 g          563 t
ORIGIN      
        1 ggatccggcg cacgcaagtc gcccgcgatc gagcgtaggg cctgcttata cggagccagc
       61 acatcgaacc gcgtcatcag gatctcgaac agggcatcac gccgactggt tgtgctctcg
      121 cgtttaggtg cccgccgcaa cacctcatcg tcgactgccc gggtgaaggc cgcaaggatt
      181 tcggatttgc tcgagaactc cttcttaaga tcgacgagtg tgacgccggt cgcttccgcg
      241 atgtcgagca gggtcatgtc tttccagggc cgctcgttcg cgagcttcat ggccgcttcg
      301 atgatccggc cgcgcagtgt ctgatcgaac atgacaagcc tccgtgccct aaagacatag
      361 ggtcgctcgg cgagtttgaa aggctgatgt gaacctcagc cactgagctc gcggctacgc
      421 ctcgccgccg cctcaacggc gcggacgagg agtggttgca ggccgtcctt cgccatgagt
      481 acctcgaggg cggctgccgt cgtgccaccc ggggaagtca cgttctgccg cagcaccgat
      541 ggctcgagcc cggactgccg caacagctcg cctgcaccgg atacagttgc atttgccaga
      601 cgcttcgcca agtcggcgtc gaggcctaca gcgcgcccag cctcggcgag gcattcagcg
      661 agcaggaaaa catacgctgg acccgaaccg gaaaccgccg tggccgcatc gatcaaggcc
      721 tcgtcctcca cccagccgac ctctcctatg gccgacagca gtgactggca catttcgcgc
      781 tggctggtgg aaacgtgagc gttggcgcaa cagacggtga tcccccgacc gatcgctgcc
      841 ggggtgttgg gaatggagcg gaccaccgcc gagccagcag gcaaatgcgc ctcgaagctc
      901 gcaatcgtct tgcccgcggc tatcgacagc accaacgtct ccggtccggc ttgggtggca
      961 agctctggga agacctgatc catgatctgc ggcttgactg ccaccaggat caccgcgggc
     1021 ggctctggca ggggggcacc cggctgccgc atctcaatgc catgccgcga aatgagctcg
     1081 gcattttcgg gcggcggcgc agggtcctgc acgatgatct gtgagggcgc taggccgcga
     1141 tcgagccatc ccgtcagcat ggccccaccc atcttgccag cgccggccag caacagcttg
     1201 cccttcaggc tcaacgacac cgcaccgaac tccacttgag taaaggataa agttgccacc
     1261 gcagcctcat ggtgcgggcc tcatccaccc caccggatct atttcggagg gctcttttga
     1321 ataacgcatc actccgatgc tcgtcatcag tgtgccgccg aggccggggt cgacgcacgc
     1381 ctccggggca agcctaagct tgaccctcgg tgtggaacat cgtggaaacg agggcctctc
     1441 gactttcctt tccggcccag acgacgaact ggaaggcttg atagtaccgt tcgcacgcct
     1501 ccagcgccgc cttcaacagg gcctccacct gcggcggcgt tgccagcgca ccgttgagca
     1561 tgaggccatg gcggaacatg atcagtcctt cctgggtcca aaaatcgaaa tggccgagcc
     1621 aaagctgctc gttgatctgc gcaataagct tgtaaatttc tggcagacgg ttgtccggca
     1681 ccttgaaatc gaaggcgcaa gccagatgca gcacctcgag atcgtcgcgc caattgagcg
     1741 agaggtgata atccgtccag ctccccgcga cgatcaacgt aacttcgtca tcggcagtgc
     1801 gatcacacgc ccagccgtgg gtatgtgcta cctgttcgac gatatcgatt ggattgctca
     1861 tgcgctgata gcctgcttcc gccgatgcca ttcccagcca tcccccttgt tattccgccg
     1921 cgtggcttga ggatctacgc tcgacatccc ctgatcccct cacgacgatc agtgccggac
     1981 agtctgtggt aaccggataa accggtcgct gagcgggagc gaccaaaaca gatgcaaggg
     2041 acgagtcgca agctcaccct cttattgtcc acgggcaaag tgatgagttt cgggcgatgc
     2101 aagcgaattc gcgctgcttt tggaggaagt gtgcgcaatc gatggaaaac ctggggatgt
     2161 cgccagccgt gtcaggcccg acacagcaag cgactcaggg cgacgcagga aagactcaca
     2221 ccctgctttt cagagcggcc aattcggctt cgagggctgc aatccgggcc tcgagcttct
     2281 cgttctcttc ccgagcacgc tgagccatag ccttgaccgc ctcgaactcg tcgcgccgta
     2341 cgacatccat ctcgcggagc acgcgctcgc cctgggcttt catgaaagac tcgaactcgc
     2401 ggcgcagacc ctgagcggcg cctgccgcgt ccgtcatcat cttcgccagc tcgtcaaaaa
     2461 accgattggt ggtctgggtc atgggattag ccgccactcg tgatctattg ggttgcatgt
     2521 cactatgggt ctcggtgcgc tccggcgcaa gcatatcggc gcacccggtt gacagtagcc
     2581 gcaccgagcc gtcaacgtcc ggcgcgcggc aatgttgctt ggcgagccgc taaacttccg
     2641 cctcaccgct tcaccgcagg caatcgatga attctttcgc gagctttctc gccattccat
     2701 ttccaaacat cgatccggtc gcgctccagc tcggcccggt caccgtgaaa tggtacgggc
     2761 ttgcctatat ggcggggctg ctcctcggct ggctctacat caggcggctg atgcgaaatc
     2821 ccgccttgtg gtcaggcggc aagccgccat tcggcctgga gaaaacggac gacctactgt
     2881 tcttcatgac gttgggcgtc gtgctgggtg gccgcctc
//
LOCUS       DASUMG010000234         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_512456, whole genome shotgun sequence.
ACCESSION   DASUMG010000234 DASUMG010000000
VERSION     DASUMG010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512456"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFX46_03905"
     CDS             <1..232
                     /locus_tag="VFX46_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5599761.1"
                     /translation="VTDDALIDWAEHLEPEALQGDLTWYSGASGRELTRPRGLLVMHL
                     FNHQTHHRGQVHCLLTQLGVKSDATDLPFMPD"
     gene            complement(245..532)
                     /locus_tag="VFX46_03910"
     CDS             complement(245..532)
                     /locus_tag="VFX46_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599762.1"
                     /translation="MRLFLAALIALGAFAVVPDDADARKRARAPYAYRDYGPPPPWAY
                     GPPPPRYGYRYRESEQSDSMECIEARNLDPGGDYASYPCWAQRALAPKKHL"
     gene            680..>2599
                     /locus_tag="VFX46_03915"
     CDS             680..>2599
                     /locus_tag="VFX46_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5599763.1"
                     /translation="MDAQVPIGYSACPHDCPSTCALEVELLGNGRIGRIRGSKENTYT
                     AGVICSKVSRYAERVHHPDRLLYPLRRKGPKGSREFERISWKDALDITAEAMLAAEHK
                     YGPEAVWPYYYAGTMGLVMRDGINRLRHAKRYSDMFASICTTLAWTGFVAGTGKLAGP
                     DPREMAKSDLVVIWGTNPVNTQVNVMTHAVRARKERGAKIVAIDIYRNGTMEQADLAL
                     CLRPGTDGALACAVMHVLFRDGYANWPYMERYTDCPRELEAHLRTRTPEWASAITGLS
                     VEEIETFARMIGTTPRTYLRLGYGFSRQRNGAINMHAASCIASVTGSWLHEGGGAFHN
                     NGAIFHWDKTMIEGLDVRDPSVRVLDMSRIGPVLTGNPYDIGDGPPITALFIQNMNPV
                     QVAPEQRLVKKGFMREDLFVCVHEQFMTPTAQMADIVLPATMFLEHDDLYQAGGQQSI
                     LFGPKLIEPPGECRSNHDVIVELAKRVGAKHPGFEMTPRELIDWTLRKSGWGTLEELE
                     EKKWIDCQPDFETSHFLNGFGYPDGKFRFRPNWADVPVGRRHDWGLSHLPPALPDHWD
                     VNEKADEKHPFKLATSPARSYLNSSFNEMPSSRAREGRPRAKIHPDDLAALGISDGAR
                     IRMGNERGEIVIHAEA"
BASE COUNT          500 a          814 c          823 g          462 t
ORIGIN      
        1 agtcaccgat gatgcgctta tcgattgggc tgagcatctg gagccagaag cgctccaggg
       61 cgacctcacg tggtactcgg gtgcgagcgg ccgtgagctg acccggccca gaggcctatt
      121 ggtcatgcac ctcttcaatc atcagacgca ccaccggggc caggtgcact gtcttctgac
      181 gcaacttggc gtcaagtcgg acgcgaccga cctgcccttc atgcctgatt agcgagactg
      241 caccttagag gtgtttcttc ggcgccagcg cccgttgtgc ccagcaggga taactcgcgt
      301 aatcgccgcc aggatcgaga ttgcgggctt caatgcattc catcgagtcg gactgctctg
      361 actcgcgata ccgataccca tagcggggcg gaggcggccc ataagcccat ggcgggggtg
      421 gcccgtagtc gcggtaggcg taaggcgcgc gggctctttt gcgcgcatct gcatcatccg
      481 gaacgacggc gaaagcgcca agcgcgatca gggcggcaag aaaaagtctc atgataaact
      541 ccattcgcca ggcgcactga gcctggcagg tatcccatcc atacaactaa cgttccgtgc
      601 gtccccgctc cgtttcgctt tcactccgtc gcgaaagccg ttagcgtcag atctggtgcg
      661 atattgattg gaacgaaaca tggacgccca ggttccgatc ggctattcgg cttgcccgca
      721 cgactgcccg tcgacctgtg cactcgaagt ggagctgctc gggaacggcc gcatcggccg
      781 aatccgcggg tcgaaggaga acacctacac ggccggcgtc atctgctcga aagtctcgcg
      841 ctacgccgag cgcgtccacc atcctgaccg cctgctgtat ccgctgcggc gcaagggccc
      901 caagggatcg cgcgagttcg agcggatctc ctggaaagat gcgctcgaca tcaccgcgga
      961 ggcgatgctc gcggcggagc acaagtatgg cccggaagcg gtctggccct actactacgc
     1021 gggcaccatg ggccttgtga tgcgcgacgg catcaaccgg ctcaggcacg ccaagcgcta
     1081 ctccgatatg ttcgcgtcga tctgcacgac gctggcctgg accgggtttg tcgccggcac
     1141 cggcaagctt gcaggtcccg atccgcgcga gatggccaag tccgacctcg tcgtgatctg
     1201 gggaacgaac ccggtcaaca cgcaggtcaa cgtgatgacc catgccgtcc gggcgcggaa
     1261 ggagcggggc gccaagatcg ttgccatcga tatctaccgc aacggcacga tggagcaggc
     1321 ggacctcgca ctttgcctgc gtccgggcac ggatggtgcg cttgcctgcg ccgtcatgca
     1381 tgtgctgttc cgcgatgggt atgcgaactg gccatacatg gagcgttaca cggactgtcc
     1441 gcgcgagctc gaggcgcact taagaacgcg gacgccggag tgggcgagcg ccatcacggg
     1501 gctttcggtc gaggagatcg aaacgttcgc gcgcatgatc ggcacgacgc cgcgcaccta
     1561 tctgcggctc ggctacggct tctcccgcca gcgcaacggc gcgatcaata tgcacgcagc
     1621 ctcgtgcatc gcgtctgtca ccggctcttg gctccacgag ggcggtgggg cgttccacaa
     1681 caacggcgcg atcttccact gggacaagac gatgatcgag gggctcgatg tccgcgatcc
     1741 cagtgtgcgc gtccttgata tgtcacgcat cggaccggtg ctgacgggca acccatacga
     1801 catcggcgac ggcccgccca ttacggcgct tttcatccag aacatgaacc cggtgcaggt
     1861 cgcgcctgag cagcggctcg tcaaaaaggg cttcatgcgc gaggacctgt tcgtgtgcgt
     1921 gcacgaacag ttcatgacgc cgacggccca gatggccgat atcgtgctgc cggcgaccat
     1981 gtttctcgag cacgacgacc tctatcaggc gggagggcag cagagcattc tgttcggccc
     2041 gaagctcata gagccgccgg gcgagtgccg ctcgaaccac gacgtcattg tcgagctggc
     2101 caagcgtgtc ggggcaaagc accccggctt tgagatgacc ccgcgcgagt tgatcgattg
     2161 gacgctgcgc aaatccggct ggggcacgct cgaggagctg gaagagaaga aatggatcga
     2221 ttgccagccg gatttcgaga cctcgcactt cctcaacggg ttcggttatc ccgacgggaa
     2281 atttaggttc cggcccaact gggccgatgt gccggtcggg cgcaggcacg attggggcct
     2341 gtcgcacctg ccgccagcac tgcccgacca ttgggacgtg aacgaaaagg cggatgagaa
     2401 gcatccgttc aagctcgcaa cttcgcccgc gcgcagctat ctcaactcgt cgttcaatga
     2461 gatgccttcg agccgcgcca gggaaggccg tccgcgggcg aagattcacc ccgatgatct
     2521 agcagcgttg gggatcagcg atggcgcgcg catccgtatg ggcaacgagc gcggcgagat
     2581 cgtcattcac gccgaagct
//
LOCUS       DASUMG010000235         3178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1074050, whole genome shotgun sequence.
ACCESSION   DASUMG010000235 DASUMG010000000
VERSION     DASUMG010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1074050"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VFX46_03920"
     CDS             complement(<1..1136)
                     /locus_tag="VFX46_03920"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599764.1"
                     /translation="MLQSSPTVRALFAVPDYRRLWTIGALVGVVRWLEFLALGVFAYQ
                     VTQSPTYVALIALVRMAPWIVLGFLIGALADYFDKQRMLAAGFLVSGVAFAAMAVLSA
                     FGLAGYGTAICAALVSGLLWTTDLPVRRRLLVEAAGVHRMTAALGFDNSSYFAARAIG
                     PILGGVAYQLLGIEGIFALSAAVYLACFYLSVGLTDVEPSPTDRAHVGNPRPSVLSLL
                     IPPRELILSRRFQVVVGVTIVFNVWCFPFLSMVPVIGEKEFGLTPAQIGALSACEGVG
                     GTIGAILIGLLAGQRPYFPFYYFGVMGFLALMLGVSVSLTLGTTIVGLLLIGASASGF
                     SAVQYALIYTMAPPDMRGRAAGFLSIFIGTSTLGFYNTGWVFSR"
     gene            complement(1307..2146)
                     /locus_tag="VFX46_03925"
     CDS             complement(1307..2146)
                     /locus_tag="VFX46_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5599765.1"
                     /translation="MTPSSRVAAGGASIPVIGYGTWPLKGEECVRGVAAALACGYRHI
                     DTAAMYQNEAEVGEGLRVSGVPREDVWITTKVWWEDISDGPLQRSAERSLRQLGIDQV
                     DLLLIHWPNKAVPLSESIRALNDAKRRGLTRHIGVSNFPSRMLEEAVALSSEPIVMNQ
                     CEYHPYLDQSKVLAACRRHGVAFASYCPLGRGSVGGVLQDPVVQDIARRVGRTPAQVV
                     LRWHIQQPGVIPIPKSANPKRIAENFAIFDFELSEDDMRRLSSLARPDGRIVTMSVAP
                     EWD"
     gene            2954..>3178
                     /locus_tag="VFX46_03930"
     CDS             2954..>3178
                     /locus_tag="VFX46_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5599766.1"
                     /translation="MSRYSFIRQCLWTAAAASVVLTTMATSASAQGFFSFLWSGGSRQ
                     VVSFPKQYAPGQIIVSFGDRRLYWIHRRGEA"
BASE COUNT          677 a         1025 c          977 g          499 t
ORIGIN      
        1 aaacgagaga acacccagcc ggtgttgtag aagccgaggg ttgatgttcc aatgaagatg
       61 gagaggaagc cagcggctcg cccgcgcatg tccggcggcg ccatcgtgta gatcaatgca
      121 tattgaacgg ccgagaagcc agaagcgctg gcgccaatca gcagcagccc gacgatggtg
      181 gttccgagag tcaatgagac cgatacgccg agcatgagcg cgaggaaccc catcacgccg
      241 aagtaataga aggggaagta cggacgctgg cccgcaagaa gccctatcaa gatggcgccg
      301 atcgtcccgc caactccctc gcaggccgac aacgcaccaa tctgagccgg cgtgagcccg
      361 aattcctttt caccgatgac cggcaccata ctgaggaacg ggaaacacca gacgttaaaa
      421 acgatggtga cgccgaccac gacctgaaac cggcggctca ggatcagctc gcggggtggg
      481 atcagcaggg aaagaacgga agggcgaggg ttccccacgt gagctctatc cgtgggagaa
      541 ggctcgacgt ccgtcagccc gacggacaaa taaaagcagg cgagatagac ggccgcgctc
      601 agggcaaaga tgccctcgat gccaagcagc tgataggcga cgccgcctag gattggccca
      661 atggccctcg cggcgaagta gctcgagtta tcgaagccaa gcgccgccgt catccgatgg
      721 acacctgccg cctcgaccag cagccggcgg cgcaccggaa gatccgttgt ccagaggagg
      781 cccgatacca gtgccgcgca gatcgccgtt ccgtagcccg ccaaaccgaa tgcagacagc
      841 accgccatcg cggcgaacgc gaccccactg acgagaaagc cggccgccag catgcgctgc
      901 ttatcgaaat aatcggcgag cgccccgata aggaatccca atacaatcca gggcgccatc
      961 ctgacgaggg cgatcagggc cacatacgtg ggagactgcg tcacctggta ggcgaatacg
     1021 ccgagcgcca gaaactccag ccaccgcacc acgccaacga gcgcgcctat cgtccacagg
     1081 cgccggtaat cgggcaccgc gaagagcgcg cggactgtgg gcgaggactg gagcacggaa
     1141 gctgcggctg gtcagagggg cgtaccggga tctcctgaca attgcccgga atgcccttca
     1201 atgtcgactg gcacccgtgc atgacacacg tgcgcagacc gtgggagctt ggcagcgggc
     1261 acccatctgg gcgacatcag cccatccggt cgacaactac ccacccctaa tcccactcag
     1321 gcgcgacgga catcgtcaca atgcgcccgt ccggccgcgc aagactggac aggcggcgca
     1381 tatcgtcctc gctaagctcg aaatcgaaaa tggcgaagtt ctcggcgatc cgcttcgggt
     1441 tggcggattt cggaatgggg atcacgcccg gctgttgtat gtgccagcgc aagacgacct
     1501 gagccggcgt gcgcccgacc cgccgggcga tgtcttgcac cacaggatcc tgcaacacgc
     1561 cgccaacgga gccgcgaccg agggggcaat aggacgcgaa ggccacaccg tgccgtctgc
     1621 aggcggcgag caccttcgac tggtcaaggt acgggtgata ctcgcactgg ttcatgacga
     1681 tcggctcgct cgagagggcg accgcctcct caagcatgcg ggaggggaag ttcgaaaccc
     1741 cgatgtgccg ggtaagcccg cgccgcttcg cgtcgttgag ggcgcggatc gactccgaaa
     1801 gcggcacagc cttgttcggc caatggatga gcagcagatc gacctgatcg atgccgagct
     1861 gccggaggct cctctccgcc gagcgttgaa gcgggccgtc tgagatgtcc tcccaccaca
     1921 ccttggtggt gatccatacg tcctcccggg gcactccgct gacccgcagg ccttcaccga
     1981 cctcagcctc gttctggtac atcgcagccg tgtcgatatg gcggtagccg catgcgagcg
     2041 ccgccgccac cccgcggacg cattcttccc ccttgagggg ccaggtgccg tagccaatca
     2101 caggaatcga tgcgccgccg gccgcgacgc gcgagctcgg agtcattgag aggctccctc
     2161 ttcacgccag ttcgaggagg tgccgatcgt ttcgaagccg accggcaaag tcaaaccttg
     2221 aattctgcac ccgcggcaga ggcggacaga gcgacggaca aaaaaagggc acccgttgat
     2281 gggtgccctc agataagacc tgcgcgtggg gggggggacg gcgcaggtca caccctaaac
     2341 ccgcaagatc caattcagtt ccactcacat ctcaaaaaaa atcgccacgc ccctgaaaac
     2401 cttaaagaac cctttcccgg cgagcccggg cccgaagaat cggacgacgc gtccggggac
     2461 gcggcgctct ggtgctctcg agcggactcc tgccggcttt accagattgg ccacccggca
     2521 ggccggtgcg ggtagctcac ccgggaacac gctcggcgga ctcctgttcc gtacgacccc
     2581 aaggcagggc cggcatctct ccatttcgaa gcggatactg cctgcgtagg ccggtcaccg
     2641 tgaccaagac tacagtaacg tcccgagata agtctttgat caagaacaaa tttgccccgg
     2701 ccgcgacctt ccctctggtg cccgcgaccg tgccagcgct tccggcacgg cccctcgccg
     2761 tgttggcatt ctgccactct cgcgtccgca atcgcgaatc ggcgccttga caagatgtcg
     2821 gacggctgac gacatgccga aataggccgt taactgcgcg tcggcataat ggctttgcgt
     2881 gtgccgttca tctctgatgc gagcggcgac aacgattccg aaggtttcga cttccgagat
     2941 tggatggtgg caaatgtcgc gttacagttt tatccgtcaa tgcctctgga ccgccgccgc
     3001 agcgagcgtc gtgctgacca caatggccac gagcgcatcc gctcagggct tcttctcgtt
     3061 cctgtggagc ggtgggtcgc gccaagtggt gtcgttcccc aagcagtatg cgccggggca
     3121 gatcatcgtc agcttcggtg accgcaggct ctactggatc caccgccgtg gagaggcc
//
LOCUS       DASUMG010000236         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_673227, whole genome shotgun sequence.
ACCESSION   DASUMG010000236 DASUMG010000000
VERSION     DASUMG010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673227"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFX46_03935"
     CDS             complement(<1..308)
                     /locus_tag="VFX46_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012757773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5599767.1"
                     /translation="MRKETNSPSQLIDAKIKGIPDWRGAVLARIRQLIKEADPDVNEE
                     VKWRKPTNPSGVPVWSHNGIICTGETYKDKVKLTLAKGALLDDPNQLFNASLDGKERR
                     A"
     gene            complement(330..1151)
                     /locus_tag="VFX46_03940"
     CDS             complement(330..1151)
                     /locus_tag="VFX46_03940"
                     /inference="COORDINATES: protein motif:HMM:NF010309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase family
                     protein"
                     /protein_id="HEX5599768.1"
                     /translation="MIHFLANEPESCDQIAAAIDIVRDVLGEAVCAAYLYGSAVAGGL
                     RPSSDLDILVVSDHSLNRDAQASIIRRLLPISGRHAIGGPARSLELSIVARPALAPWR
                     YPPNIELQYGDWMRAEFERGELPEWPKADPDVAILIEAARRASVPLFGEPVASVLEPV
                     PQTDLVRAMRDTVPLLMPGILEGSDIRNGLLTLARIWTTLATGEIRPKDEAASWVLVH
                     LPEEHKAVLAHALAAYRGESLESWYALGPRLRPHVDYVVAQIYGLATTQLRNDQS"
     gene            complement(1171..1641)
                     /locus_tag="VFX46_03945"
     CDS             complement(1171..1641)
                     /locus_tag="VFX46_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX5599769.1"
                     /translation="MADKSSRQPRKPAKKLAPKAASEQPVLLSGGNPQIPKGYGDAPV
                     QAYIAAMPGWKQNVGRRLDAIISRTVPGVSKAVKWNSPFYGVEDNVWFLSFHCFTRYV
                     KVAFFRGASLTPIPPGKSKQEHVRYLDIYEDDPLDEAQFADLVRQAAALPGERM"
     gene            1999..3177
                     /locus_tag="VFX46_03950"
     CDS             1999..3177
                     /locus_tag="VFX46_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX5599770.1"
                     /translation="MRWKSFALVGLCTIVSVGTAATILALDEAGFIRLVAVEENAPQE
                     VALAVPEPQPVTPLAVPAPQPATPRAAPKTIGRFTYASLDPAELVPSPYLREQDVTEE
                     PEELDSPDLAEVPDEADDGADDVLNNNPPAELETTDSWHVDVKPEEREAQSAETPSRS
                     APQTQRATRPRGVYRTPALEKRLAQISPGATARLWKKFSSVKAAWPPSEIALVAIKDE
                     KVIELFARQDENAEWQFVHRYPVLAASGGPGPKLRQGDKQVPEGVYRISLLNPNSRYH
                     VSLRVNYPNEFDREKAKEDGRTNLGGDIMIHGKDKSAGCLAVGDEAAEELFVLAAHIG
                     LDNIKLIIAPTDFRRRGIPPVSPHQPKWLPELYTQVAEAMTEFKSPPPRISLLSLFGI
                     "
BASE COUNT          672 a         1055 c         1062 g          643 t
ORIGIN      
        1 gcgcgccttt ccttgccgtc caggctggcg ttgaacagct gattagggtc gtccagaagg
       61 gcgcccttag ccaaagtgag cttcaccttg tccttgtacg tctcgcccgt gcagatgatc
      121 ccgttgtgcg accacactgg aacgccggag gggttcgttg gcttccgcca tttcacctct
      181 tcgttaacgt cgggatcagc ctctttgatg agctgacgga tgcgcgcgag caccgcgccg
      241 cgccagtcag ggattccctt tatttttgca tcaatcagct gagacgggga gttagtctct
      301 ttcctcattt cataattgct gccgctttct caggattgat cgttcctcaa ttgcgtagtc
      361 gcgagtccat agatttgcgc gacgacgtaa tcaacgtgcg gccgcaatcg cggaccgagc
      421 gcgtaccaac tctccaaaga ctcaccgcgg taagccgcta gggcgtgcgc aagaacggct
      481 ttgtgctcct ccggtagatg aacaagaacc cagctcgccg cctcatcctt cggacggatc
      541 tcgccggtcg ctagcgttgt ccagatacgc gccagcgtaa gcaacccgtt ccgtatgtca
      601 ctcccctcca ggatgcccgg catgagaagt gggacagtgt cgcgcatggc cctgacgaga
      661 tcggtctgcg gcaccggttc caggacggaa gcaactggct ctccgaacag tggcacagac
      721 gcacggcggg ccgcctcaat gagaatcgca acgtctggat cggccttcgg ccattccggc
      781 agctcgcccc tctcgaattc ggcgcgcatc caatcgccgt actgcagttc gatgttcggc
      841 gggtagcgcc acggtgcgag tgccgggcgg gcgacgatcg aaagttcaag cgaacgggcc
      901 gggccgccga tggcatgtcg gccggaaatt ggcaaaagtc gccggataat agaggcctgc
      961 gcgtcgcggt tcagcgagtg gtcgctaacg acgagaatgt cgagatcgct gctcggacgc
     1021 aacccgcccg cgacggcgga cccataaaga taagccgcgc ataccgcctc gccgagaaca
     1081 tcacgaacga tgtcgatggc ggcggcgatc tgatcgcacg actctggctc attcgcaagg
     1141 aagtggatca tggctgcgaa aactgatctc tcacatcctc tcccccggca acgccgctgc
     1201 ctgcctcacc aaatccgcaa actgcgcttc atctagcgga tcatcctcgt agatatcgag
     1261 gtaccgcacg tgctcctgct tcgacttgcc cggcgggatc ggggtcaggg aagcgccgcg
     1321 gaagaaagcg accttcacgt acctggtgaa gcagtgaaag ctgaggaacc agacgttgtc
     1381 ctcgacgcca tagaagggcg agttccattt gacggcctta ctcactccag gaacggtgcg
     1441 ggagatgatc gcgtcgagac ggcggccgac attttgtttc cagcccggca tcgccgcgat
     1501 gtaggcctgc accggagcgt cgccgtagcc cttcgggatc tgaggattgc cgcccgagag
     1561 gaggacaggc tgctcagagg ccgctttggg tgccagtttc ttcgctggct tcctgggctg
     1621 ccgggatgat ttgtcggcca tgaacctcgc tccaatcgaa cgcgtgatcg tcgtagctac
     1681 actcagatta cggcgcttct gcagggactt gcagcaaacg cttcgcgcaa atagaatctg
     1741 cgctctgtct ctcatctccg agttgcaaaa cttgtcgtcg ccgcaaacgt tgatctgcaa
     1801 ctctaaggtg cgacgtctga ttttccagca gaaattccgt cgtccgcggg tcaggcgccg
     1861 cagagctgtc gtctttccac tcgccttttt gccttaaaga atcggctaca tgcgctaaag
     1921 tctgcgagac gcagcgaaag ccgagccgat ggctgatgcc gaacgagtgc cggccgctgt
     1981 ggtgggggtg gtgaaaggat gagatggaaa agcttcgcgc tcgtcgggct ttgcacgatc
     2041 gtctccgttg ggactgcggc gaccatactc gccctcgacg aagccggctt catccgactt
     2101 gttgctgtcg aggagaatgc gccccaagag gtggcgctgg ccgttcctga accgcaacct
     2161 gtgacgccac tggccgtccc tgcgccacaa cccgcgacgc cacgggctgc gccgaagacc
     2221 attgggcggt tcacatacgc gtcgctggac ccagcggagc tggtaccgtc gccgtatctt
     2281 cgggagcagg atgtcacgga agagcctgag gagctggact ctccggattt ggcggaggtg
     2341 ccggatgagg ctgacgatgg cgcggacgat gtgctgaaca ataatccccc cgcggagctc
     2401 gagacgaccg actcgtggca cgtggacgtc aagccggaag agcgcgaggc gcaatccgcc
     2461 gagacgccat cgcgcagcgc gcctcagacc caacgggcga cgcggcctcg tggcgtgtac
     2521 cggacaccag ccttggagaa gcgcctggca cagatctccc cgggtgccac cgcgcggctc
     2581 tggaagaagt tcagctcggt gaaggcagcg tggcctccgt ccgagattgc cctggtggcc
     2641 atcaaggatg aaaaggtcat cgagctgttc gcgcgtcagg acgaaaacgc cgaatggcag
     2701 ttcgtgcacc gctacccggt tctggccgcg agcggtggcc ccggcccgaa attgcgtcag
     2761 ggtgacaagc aggtgccgga gggtgtgtat cgcatctcgc tactcaaccc caacagccgc
     2821 taccacgtct cgctgcgggt caactacccg aacgagttcg atcgcgagaa ggccaaggag
     2881 gatggccgca ccaacctggg cggcgacatc atgatccacg gcaaggataa gtcagccggc
     2941 tgcctggccg tgggggatga ggccgcagag gagctgttcg ttctcgcagc gcacatcggg
     3001 ctggacaaca tcaagctcat catcgcgccc acagatttca ggaggcgcgg aataccgccc
     3061 gtctcgcctc atcaaccgaa atggctgcct gagctctaca cccaggtcgc tgaggcgatg
     3121 acggagttca agtcgccgcc gccgcggatc agcctgctgt ccttgttcgg catttgacga
     3181 aggcggcacg gttcgccgtg ggaccgcgaa aggacttcgg cgaaagctct gagtggcggt
     3241 cggttgggga atagcaagga ccggttttcg gcgccgctaa ctacggtcca gcggattgct
     3301 gcttcatccg ctcgctgcgc gcgcgcagca gctcgaccac gagcaggagc agaacggcca
     3361 ccaacgtcag catgactgcg acggcggcga tcgtcggatt gaagctgtcg cgcaagccgg
     3421 agaacatctc cc
//
LOCUS       DASUMG010000237         3042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_592305, whole genome shotgun sequence.
ACCESSION   DASUMG010000237 DASUMG010000000
VERSION     DASUMG010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592305"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX46_03955"
     CDS             complement(<1..508)
                     /locus_tag="VFX46_03955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5599771.1"
                     /translation="MQIGYGTQRVTFARYTGTGFDRICEKGNLMRRGLLGQLAAAAAL
                     AGVVAPGIAFAAAPECQNTVSFSRWLAEFRQEAAAAGISRATISAALDGMTLDRSIIA
                     RDRRQTFFAQSFLDVVQKLATTHRIRAGTRAMQRHNAIFQRAEREFGVPASVITAFWA
                     LESDFGAGV"
     gene            1108..1251
                     /locus_tag="VFX46_03960"
     CDS             1108..1251
                     /locus_tag="VFX46_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HEX5599772.1"
                     /translation="MITETSGGNPAMDYDEHYRTYRGFLKGAKYLVGLVIITLVLMAI
                     FLL"
     gene            1376..2515
                     /locus_tag="VFX46_03965"
     CDS             1376..2515
                     /locus_tag="VFX46_03965"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX5599773.1"
                     /translation="MVVIAVTAEPANETRVAISPDTVKRLAGAGCNIKIESGAGLRSR
                     FSDDLYEAQGAHIARSREDALSGADILLKVRRPSLDEVKALKPGAIVAAMLDPYGDRA
                     GLDALAATGVTLFSMEFMPRISRAQSMDVLSSQANLAGYKAVVNAAAMFEQAMPMMMT
                     AAGTVPAARVFVMGVGVAGLQAIATARRLGAVVTATDVRPATKEQVLSLGAKFIAVED
                     EEFKQAETAGGYAKEMSKEYQAKQAALVADHIKKQDIVITTALIPGRPAPKLVSAEMV
                     RSMKPGSVIVDLAVERGGNVDGAKAGQVAEVNGVKIVGFTNVAGRLAASASGLYAKNL
                     LTFLEILIDKKEKKLAVNWDDEIVRATALTRDGLVVHPNFMPKTA"
     gene            2564..2887
                     /locus_tag="VFX46_03970"
     CDS             2564..2887
                     /locus_tag="VFX46_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HEX5599774.1"
                     /translation="MTGTALDAVDPFVFRLSIFVLAVFVGYYVVWSVTPALHTPLMSV
                     TNAISSVIVVGALLAVGVGLVANDNGPIWARALGFVALVFASINIFGGFLVTQRMLAM
                     YKKKG"
     gene            2893..>3042
                     /locus_tag="VFX46_03975"
     CDS             2893..>3042
                     /locus_tag="VFX46_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX5599775.1"
                     /translation="MSANLAALLYLASGVLFILALRGLSSPESSRRGNMFGIVGMTIA
                     VLTTLA"
BASE COUNT          560 a         1002 c          935 g          545 t
ORIGIN      
        1 cgacaccggc tccaaaatcg ctttccagag cccagaacgc cgtgatcacg gatgccggga
       61 cgccgaactc ccgctcagcc cgctggaaga tggcattgtg gcgctgcatg gcccgggtgc
      121 ccgccctgat acggtgggtg gtggcgagct tctgcacgac gtcgagaaag gattgggcga
      181 agaaggtctg gcgccggtcg cgcgcgatga tgctccgatc cagggtcatc ccgtcgagcg
      241 ccgcagagat cgtcgctcgc gagatgcccg cggcagccgc ttcctgccgg aattcggcca
      301 gccagcggct gaaactgact gtgttctgac actccggcgc cgccgcgaac gcgatccccg
      361 gcgcgacaac accggccaat gccgcagctg ccgccaactg gcctagtaaa cccctgcgca
      421 tcaaattgcc tttctcacag attcggtcga aacccgttcc cgtgtatcgg gcaaatgtga
      481 cgcgttgcgt gccataccca atctgcactc gcacgctcgg agcaggccta catgctggtg
      541 gcgacttccg gaaacttctg ctgaacaatc gccggcgccc aaacaacaga gcaaacaaga
      601 tcgtcgccgg cgaactctga ttcacaaatg acgtcgcaga gtcggcttag tcctcgcaga
      661 ggtgacttcc gcaggtggcg aggatctctt gcgcggtgtt cgcccgctcc agagcctgcg
      721 tcgggggagg gccaagaagc gccatcacaa tgccccccaa cagcaaggct accgcaaagc
      781 ccgcgcaaaa ctccaatctg ttcttcattc gcatccttct tgccctctaa gacggactag
      841 cagctccctc ccaggcacgc gaagtgccac tcccccgcgc cccgtattta gcggtacgtg
      901 accgccctcg gcgagcccta tcagaggcgc ctgtgcacaa ggcggatctc ctgagggtgt
      961 ttcatgagca gatgcatcaa tttacaggct tgaacccatt gcctccctgg gataggtcca
     1021 accgtgcgca aggacgcctt cggcccagaa ggggatcact tgggggccgg agttcggtca
     1081 aatgagctgg atgtcgtgga gggggaattg atcactgaga cctcgggtgg caatcctgcc
     1141 atggattatg acgagcatta ccggacctac aggggcttcc tgaagggcgc gaagtacttg
     1201 gtcggtctcg ttatcatcac gctggtcttg atggccatct tcctgcttta gccaccatcc
     1261 tgctcgcatg gcgaaggttt cgtgcccgga attctgcggc acgatccggg ttgttgcgtg
     1321 ccagcagctc gccaactagc caaggacgtc cgggtcctcc acacaacggt gccccatggt
     1381 agtcattgcc gtaacggccg aaccagcaaa cgagacgcgg gttgccatct cgcccgatac
     1441 agtgaagcgg ctggcaggtg ctggctgcaa catcaagatc gagagcggtg cgggactgcg
     1501 ctcacgcttc tccgatgacc tttacgaggc ccaaggcgcg cacatcgctc gctccaggga
     1561 ggacgcgctc tccggtgccg acatcctgct caaggtccgc cgcccatcgt tggacgaggt
     1621 gaaggccctc aagcctggag cgatcgtcgc tgccatgctc gacccatatg gcgaccgcgc
     1681 cggccttgat gctctcgcag caacgggtgt gacgctcttc tccatggagt tcatgccgcg
     1741 catttcgagg gcccagtcca tggacgtgct gtcgagccag gccaacctcg ccggatataa
     1801 ggcggtcgtg aacgcagccg ccatgttcga gcaggccatg ccgatgatga tgaccgccgc
     1861 aggtacagtc cccgccgcgc gcgtgttcgt catgggcgtt ggcgttgccg gcctccaggc
     1921 tatcgccacg gcgcgccgcc tcggcgcggt cgtaaccgcg acggacgtcc ggccggctac
     1981 aaaggagcaa gtgctctcgc ttggcgcgaa gttcatcgcg gtcgaggacg aagaattcaa
     2041 gcaggccgag accgccggcg gctacgccaa ggaaatgtcg aaggagtacc aggccaagca
     2101 ggccgcgctg gtcgccgacc acatcaagaa gcaggacatc gtcatcacca cggcgctgat
     2161 ccccggccgc ccggcgccga agctcgtctc cgccgagatg gtgcgctcga tgaagcccgg
     2221 ctcggtgatc gtcgatctcg ccgtcgagcg cggcggcaat gtcgacggcg cgaaagcagg
     2281 gcaggtcgcc gaggtcaacg gcgtcaagat tgtcggcttc accaacgttg cgggccgtct
     2341 cgcggcctcg gcttcgggcc tctacgccaa gaacctgctc acgttcctcg aaatcctgat
     2401 cgacaagaaa gagaagaagc tcgcggtcaa ctgggacgac gagatcgtcc gcgccaccgc
     2461 gctgacgcgc gacggcctgg tggttcatcc gaatttcatg cccaaaaccg cctagcagga
     2521 aaccgcacag agagcgccgc cgacgcgcaa aggagccttc gccatgaccg gaaccgcact
     2581 cgacgccgtc gacccgttcg tcttccggct gtcgatcttc gtgctggcgg tgttcgtcgg
     2641 ctactacgtg gtctggtcgg tgacgccggc gctgcacacg ccgctgatgt cggtgaccaa
     2701 cgcgatctcc tcggtgatcg tggtcggcgc gctgctcgcg gtcggtgtcg gcctcgtcgc
     2761 caacgacaac ggcccgatct gggcgagggc gctgggcttc gtcgcgctgg tgttcgcctc
     2821 catcaacatc ttcggcggct ttctggtgac ccagcgcatg ctcgccatgt acaagaagaa
     2881 gggctgacgc cgatgtcggc gaacctcgcg gcgctgctct atctcgcgtc cggcgtcctg
     2941 ttcatcctgg cgctgcgcgg gctgtcgagc ccggagtcga gccgccgcgg caacatgttc
     3001 ggcatcgtcg gcatgacgat cgccgtcctc accacgctcg cc
//
LOCUS       DASUMG010000238         2234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_914362, whole genome shotgun sequence.
ACCESSION   DASUMG010000238 DASUMG010000000
VERSION     DASUMG010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914362"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VFX46_03980"
     CDS             complement(<1..707)
                     /locus_tag="VFX46_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5599776.1"
                     /translation="MDINLARTFLTVAETGSFIEAARKMNITQSTVSARVKVLEDLLG
                     RPLFQRSKYGASLTAAGEQFQKHALALVRIWQHAQLEVGLSGQHRDHLSVGGPLSLWE
                     GFLLKWIAWLRSSIPDIAVSTTVGQSAALTQRLLEGTLDLAVVYRPMQPPGLIVEHLF
                     DEEFVLVTSAKSTSRRIGSDYVFVNWGPDFQQDHAAAHPELTSPGLNMELGPLALSYV
                     LNNPASAYFPMRLVKHH"
     gene            1030..1371
                     /locus_tag="VFX46_03985"
     CDS             1030..1371
                     /locus_tag="VFX46_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599777.1"
                     /translation="MSRIAALMVGVAAVVLGAEAAAAVSLTNRDDRQHKVTIIEGERR
                     QDHTLAPSAVLEGICLKGCVIRLNDSEEDEYELEGNEVVSIEEGFVYYDGPDVPEQPA
                     QGEEGEGAGKQ"
     gene            complement(1483..2163)
                     /gene="lipB"
                     /locus_tag="VFX46_03990"
                     /pseudo
     CDS             complement(1483..2163)
                     /gene="lipB"
                     /locus_tag="VFX46_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473778.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
BASE COUNT          456 a          676 c          707 g          395 t
ORIGIN      
        1 atgtgatgct tcacgaggcg catcggaaaa taggccgaag cgggattgtt gagcacgtag
       61 gagagcgcga gcggcccaag ctccatattg aggcccgggc tcgtcagctc agggtgagcc
      121 gccgcatggt cctgctgaaa atcggggccc cagttgacga acacgtagtc gctcccgatg
      181 cgccggctcg tggacttggc gctcgtcacg agcacgaatt cctcatcgaa aaggtgctca
      241 acgataagcc ccggcggctg cattgggcgg tagaccacgg caaggtccag cgtgccttcg
      301 aggagacgct gcgtcagcgc ggcggactgg ccgaccgtcg tcgaaacggc aatatccggg
      361 atgctgctgc gcagccaggc gatccacttc agcagaaaac cttcccacaa gctcaacggt
      421 ccgccgaccg aaaggtggtc tcgatgctgg ccggacaggc cgacctcgag ctgcgcgtgc
      481 tgccaaattc gaaccagcgc caacgcgtgt ttctggaact gctcgcctgc ggccgttagc
      541 gacgcaccat acttggaacg ttggaacagc ggccgcccca agaggtcctc gagcaccttg
      601 acccgtgcac taaccgtcga ctgcgtgatg ttcatcttac gggcggcttc gatgaagctg
      661 ccggtctcgg cgacggttag aaaagttcga gcaaggttga tgtccatggc cgaagtgagc
      721 gcggcatcga cgccacgctt ctttgtaggc gtttctccct attcccaaga cctatcgaaa
      781 agttcgatag aagtcaccgg aaaattccgt ttgtggactc acttgaatct acccacattg
      841 cttcgcgact cgatgaaaac tcgagtcaag cgccctcatg ggtgagaagc agagagagga
      901 attgagacat gaaggcgaag acagcgaaag ccgccgcgaa gaagcctgca gccaaggctc
      961 ccgcgaaaaa ggcggctgcg aagaagaaga agtaagattc gtttcaagcc tgctgccgcg
     1021 gagccagaga tgtcgcgaat cgcagcgttg atggtgggtg tggcggcagt tgtgttgggt
     1081 gcggaggctg ccgccgcggt atccctgacg aaccgcgatg accgccaaca taaggtgacg
     1141 atcatcgagg gtgagcgccg tcaggaccac accttggcgc catcggcagt cctcgaaggg
     1201 atctgcctga agggttgcgt gatacggctc aacgacagcg aggaagacga gtacgagctc
     1261 gaggggaacg aggtcgtctc catcgaggaa ggcttcgtct attacgacgg ccccgacgta
     1321 ccggaacagc ccgctcaggg cgaagaaggc gaaggggctg ggaagcaata ggcaggcttg
     1381 cgtacgagga gctcagggcg cgaatgcgcc ttcgggctcc tttcctttgc cgaatggctt
     1441 caccttaagt tcggtacgca ggcggagcat ggcggcttga tccgctgccg gcggcgcgac
     1501 cggccggata ggaccaattt ttctttggaa aacctccgcc agcgcggcgt ccacttcgct
     1561 catagtgacg tcacggccaa ggtctttcag gcttgtgacg ccatgctcac gcaccccaca
     1621 gggcacaatt ccgccgaaat gactgagatc gggtgagacg ttcaggctca gcccgtggaa
     1681 gctgatccac cggcggatcc gaatgccgat ggctgcgatc ttgtcctcgg cctgctcacc
     1741 cttgtccggc cggcgcaccc acacccctac cctatccggc cggcactcgc cttggacgcc
     1801 gaacagggcc agcgtatcga tgacccagct ttcgagcatc cggatgaagg tccggatgtc
     1861 gccaacccgg cggcgcacat cgagcatgac gtagacgacg cgctggccag gtccatggta
     1921 agtgagctgc cctccccggc cggcgcggta gacggggaac cggccgggat cgatcaggtc
     1981 ctcccatcgg gtgctggtgc ccgccgtgta gagcggcgga tgctcgagca gccaaatgag
     2041 ctccggcgcc tggccggccg cgatggcggc tgcgcgctcc tccatcacgg cgaccgcctc
     2101 ggcatagtcg acgggcgcag ggccgctgac ggcccactcg atcggctggt ctcctaacgg
     2161 catgctggct tgcacttgag tgattcgctg gcgccctcgc actgcgcctc ggatgtctcc
     2221 atatcataga tata
//
LOCUS       DASUMG010000239         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_432022, whole genome shotgun sequence.
ACCESSION   DASUMG010000239 DASUMG010000000
VERSION     DASUMG010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432022"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFX46_03995"
     CDS             <1..922
                     /locus_tag="VFX46_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5599778.1"
                     /translation="ADYSEDLRIGPEILKSNIEAYRKIRNTLRWMLGNLAHYDEKLKV
                     RPREVPGLDRYMLHRLAELDVLVRQGYEAYDFKRVFHALSNFCVNDLSAFYFDIRKDT
                     LYCEPYSSKKRRACLMTLNEIFNCLTVWLAPIICFTAEEAWLARYPSEDGSVHLQVFR
                     RIPKAWRDDALAKKWEQVRRVRRVVTGALEIERAAKNIGSSLEAAPDIYVADEALLDA
                     LEDVDMAEVAITSAAQIVPSAPPVGAFTLPDVPGVGVVARRAQGRRCERSWRIVPDVG
                     SDPEYPELSARDAAAVREFEKRNIAAAAAE"
     gene            927..1445
                     /gene="lspA"
                     /locus_tag="VFX46_04000"
     CDS             927..1445
                     /gene="lspA"
                     /locus_tag="VFX46_04000"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX5599779.1"
                     /translation="MPQEVKPGWLWGPFSRLGLSIALITLIIDQAHKWWMLNVYRIEE
                     KGRVSVLPFLDLVYVKNLGVSYGLGAGVIGQGALVAFALIASLGLAVWLARGEMCRLT
                     AVSIGLLIGGAIGNAIDRLHLGGVADFFLLHAYGWSWYVFNIADAAIVAGVAGLLYDA
                     LRPSRNRAANDP"
     gene            1497..1949
                     /locus_tag="VFX46_04005"
     CDS             1497..1949
                     /locus_tag="VFX46_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599780.1"
                     /translation="MAKTGREKRLAVAASAMLASLALTGCGYDVQFNGAIFEAMGLAN
                     NGGPREEPKLPPRAGLVLPPDTQRLPEPGEAQAALPPPGEAWPEDPEERRVAAAEELD
                     RQHQEFCEKALWEARARGQPDAQITGPKGSCNPSILRNLTGKDITTRK"
     gene            2286..>2774
                     /locus_tag="VFX46_04010"
     CDS             2286..>2774
                     /locus_tag="VFX46_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5599781.1"
                     /translation="MTLALAQRIEAQTSERRASEFTLSNGMQVVVVPDHRAPVVTHMV
                     WYRVGAADEPPGVSGIAHFLEHLMFKSTEKIASGEFSRIVSRMGGQDNAFTSQDATAY
                     FQRIAKDRLAKVMEMEADRMVNLRLTEAEVLTERDVILEERRSRVENNPQAVLDEQMN
                     AVL"
BASE COUNT          508 a          816 c          925 g          525 t
ORIGIN      
        1 cgcggattat tcagaggacc tgcgcatcgg gccggagatc ttgaaatcga acatcgaggc
       61 ctatcgaaag atccgcaata cgctgcgctg gatgctgggc aacctcgccc attacgacga
      121 gaagctgaag gtgcggccgc gagaggtccc ggggctcgac cggtatatgc tgcaccggct
      181 cgccgagctc gatgtgctcg tgcgccaggg ctatgaggcg tacgatttca agcgggtgtt
      241 ccacgcgctg tcgaacttct gcgtcaacga tctgtcggcg ttctacttcg acatccgcaa
      301 ggacacgctt tattgcgagc cctattccag caagaagcgg cgcgcctgcc tgatgacgtt
      361 gaacgagatc ttcaactgtc tgacggtctg gctggcgccg atcatctgct ttacggcgga
      421 ggaggcatgg cttgcccgat atccgtcgga ggacggatcg gttcacctgc aggtcttccg
      481 gagaatcccg aaggcttggc gtgatgatgc gctggcaaag aagtgggagc aggtccgccg
      541 cgtgcggcgc gttgtgacgg gggcgctgga gatcgagcgg gcagcgaaga acatcggctc
      601 gagcctggag gctgcgcccg acatctatgt cgccgacgag gcgctgctcg atgcgctcga
      661 ggacgtggac atggccgagg tggccatcac gagcgcagcc cagatcgtgc ccagtgcgcc
      721 gcctgttggc gccttcacgc tgcctgacgt accgggtgtt ggcgttgtgg cgcgccgggc
      781 gcagggccgc cggtgtgaac ggtcctggcg tatcgtgccg gatgtcggat ccgatccgga
      841 atatccggag ctatcggccc gcgacgcggc tgcggttcgc gagttcgaaa agcgcaacat
      901 cgccgctgcg gccgctgagt agcagaatgc ctcaggaggt caaaccgggg tggctgtggg
      961 gaccgttttc gcgcctgggc ctttccattg cactcattac gctgatcatc gatcaggcgc
     1021 acaaatggtg gatgctcaat gtctaccgca ttgaggagaa gggcagagtt tctgtgctgc
     1081 cctttctcga cctggtctac gtcaagaatc tcggcgtcag ctacgggctc ggagcaggtg
     1141 ttatcggcca gggagccttg gtggcctttg cactgatcgc ctcgcttgga cttgcagtct
     1201 ggctggcacg cggtgagatg tgccgcctga cggcggttag catcggattg ctgatcggcg
     1261 gcgctatcgg caacgccata gaccggctac atctcggtgg tgtggcggat ttcttcctac
     1321 tccacgccta cggatggtcg tggtacgtct tcaatatcgc tgacgcggcg atcgtggccg
     1381 gtgtggcagg tttgctatac gacgcgcttc ggccgagtcg caatcgcgcc gcaaatgatc
     1441 cataaaggtg cgatcagaaa cgattctttc cgacgcgact gcttcggggg cggctaatgg
     1501 cgaagacggg tcgagagaag cggttggcgg tggccgccag cgcgatgctg gcatcactgg
     1561 ccctcacggg gtgcggttac gacgtccagt ttaacggcgc catcttcgag gcgatgggtc
     1621 tggcgaacaa cggtgggccg cgcgaggagc caaagctccc gccacgagcg ggcctcgttc
     1681 ttccgccgga cacccaacgg ctgccagagc ctggtgaggc ccaagccgcg ctgccgccgc
     1741 cgggcgaggc ttggcccgag gatccggagg agcgccgggt ggcagctgcc gaagagctcg
     1801 atcgacagca tcaggaattc tgcgagaagg cgctatggga ggctcgcgcg agagggcagc
     1861 ctgacgcgca gatcaccggc ccgaaaggat cttgcaatcc gtcgattctg cggaacctca
     1921 ccggcaagga cataacgacg cggaagtagc gcatgcaact gcggctcttg caatggccgc
     1981 aatcttggta tgtgttggca cgcgaccgcc gcggatcttg agcgggtgga gggtgtcgcc
     2041 gggttataca ccttggccgg actcaagcac cgacccggcg gctttcgtgc tctacattgt
     2101 ttgacaacgc tggtctcgaa cccgacataa gctcattggc aaggggcgct catctcagcc
     2161 ccgtgccagc tcctggcgcg ggaatccgcc cgagacatgc ccatctccgg gaccgcatct
     2221 gtgcataagg acagacgatg gccacacgcg ttcgggcctc acttgcaacc atcttggcga
     2281 tcacaatgac gcttgctctt gctcagcgaa ttgaggcgca aacgagcgaa aggcgcgcct
     2341 cagagttcac actctccaac ggcatgcagg tggtagtcgt tcccgaccac cgtgcgcccg
     2401 tagtgacgca tatggtgtgg taccgcgtgg gtgcggccga cgagccccca ggcgtttcgg
     2461 gtatcgcgca cttcctggaa cacctgatgt tcaagtcgac ggagaagatc gcctccggtg
     2521 agttctcgcg catcgtctcg cgcatgggcg gccaggacaa tgcgttcacc agccaggatg
     2581 cgaccgcata cttccagcgc atcgccaagg atcggctggc caaggtgatg gagatggagg
     2641 ccgaccggat ggtcaacctg cggcttaccg aggccgaggt gctgaccgag cgggacgtga
     2701 ttctcgagga gcgccgctcg cgcgtcgaga acaaccctca ggctgttctc gacgagcaga
     2761 tgaacgcggt gctg
//
LOCUS       DASUMG010000240         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_432333, whole genome shotgun sequence.
ACCESSION   DASUMG010000240 DASUMG010000000
VERSION     DASUMG010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432333"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFX46_04015"
     CDS             complement(<1..746)
                     /locus_tag="VFX46_04015"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5599782.1"
                     /translation="MHPAYSVIFFTTASGAGYGLLIWLGVFGPLGWVPQTSAFGLVGL
                     GLALLLITGGLLSSTAHLGRPERAWRALSQWRTSWLSREGVAAIATYVPAGLFGIGWV
                     FLERTDGFFALMGVLAAALAVATLICTGMIYASLPTIRAWHQPFVVPVYIVLALATGA
                     ILLNFLLLLFGIATDWAPLLAILAIAAAWILKTASWSRVDNAERSYTIEQATGLGRFG
                     KVRVLEQPHTQPNYVMREMGYQIGRKHAQKL"
     gene            complement(1077..1829)
                     /locus_tag="VFX46_04020"
     CDS             complement(1077..1829)
                     /locus_tag="VFX46_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5599783.1"
                     /translation="MTSLPETTAKKLGLVIDLDTCVGCQACATSCKEWNTGGYSAPLT
                     DQDPYGADPHGAWLNRIHTYEVVEGSSSRTVHFPRSCLHCETPACVTVCPTGASYKRA
                     EDGIVLVNPDTCIGCKLCSWACPYGAREYDYADGVMKKCTLCIDRIYNETLAPEDRIP
                     ACVRACPTGARHFGDLGDPNSAVSVLVAERGGYDLLPETGYKPVNKYLPPRPRRDATT
                     TPAVSGSSTVEAGDDGSLSARFFAWADKMLSR"
     gene            complement(1826..>2299)
                     /locus_tag="VFX46_04025"
     CDS             complement(1826..>2299)
                     /locus_tag="VFX46_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX5599784.1"
                     /translation="LRQITAQNRLYMHRDRARALGVADDDWVWVTSAIGRVKCQVKLM
                     EGVNPDTVWTWNAIGKRAGAWGLAPDAPEATRGFLLNHLISELLPEREGGYRFSNSDP
                     ITGQAAWYDLRVRIERAKPEEAGESWPRFEPLPNPYGEHRPVVSTFGAEFREAAE"
BASE COUNT          458 a          742 c          722 g          377 t
ORIGIN      
        1 agcttctgcg catgcttgcg gccaatctga tatcccattt cgcgcatgac gtagttgggc
       61 tgcgtatgcg gctgctcgag cacgcggacc ttgccgaacc ggccaaggcc cgtcgcctgc
      121 tctatcgtgt acgaacgctc cgcattatcg acccgggacc acgacgcagt cttcagtatc
      181 caggctgctg cgatcgccag aatcgcaagc aaaggcgccc agtccgtcgc gatgccgaaa
      241 agcagaagga gaaaattgag aagaatggcg cccgttgcta gggcgaggac gatgtaaacg
      301 ggcaccacga acggctggtg ccatgcgcgg atcgtgggaa gtgacgcgta gatcatcccc
      361 gtgcagatga gcgtcgccac ggccagagcc gcggccagca cacccatcag ggcgaaaaaa
      421 ccatccgttc gctccaggaa aacccaacca atcccgaaca gtcctgccgg aacataggtt
      481 gcaatggccg cgacgccttc gcgggatagc caggaggtcc gccactgaga cagcgctcgc
      541 caagctcgct ccggacggcc aaggtgcgcc gtcgacgaca gcaagccgcc ggtgatgagc
      601 aggagtgcca gccctagccc gaccaagccg aaggcagacg tctgcggcac ccatccgagc
      661 gggccgaaaa ctcccagcca gatgaggagg ccgtaaccgg cccctgatgc ggtcgtgaaa
      721 aagattaccg agtaggcggg atgcattgga tcagcctgtt ggaataggtc tcatcgcccc
      781 tcccctcagc gggatgggca cgtgaagtaa ggcatgatgg aacaggtggg acggggccac
      841 agatgactcg gtcgcgtcag agcagcccca cctccaatcc ctccccgcaa gggcgacggg
      901 agcatcctcc cgcatcgctg gagctacgga gatacgcgag cagcacacac catcagtcat
      961 cccggtgctt gtcaccggga tccacgttgc cgctagctcc agcgcctgcg gatggctgga
     1021 tcccgggcac gaggcccggg atgacatggt gtctggacaa tccggccaca caagcatcac
     1081 cgcgacagca tcttatcggc ccaggcgaaa aagcgggccg agagtgaacc atcgtcgcca
     1141 gcttcaaccg tcgagctgcc cgagactgct ggtgtggtgg tcgcatcgcg gcgcggacgg
     1201 ggcggcaggt acttgttcac gggcttgtag cccgtctccg gaagcaagtc atagccaccg
     1261 cgttccgcaa cgagcacaga aacggcgcta ttcggatcac cgagatcgcc gaagtgccgt
     1321 gcgccggtcg ggcaggctcg cacgcaggcc gggatgcggt cctcgggcgc cagcgtttcg
     1381 ttgtagatgc ggtcgatgca cagcgtgcac ttcttcatca cgccgtcggc gtagtcgtac
     1441 tcgcgcgcgc cataggggca ggcccaggag cacagcttgc accctatgca cgtatcggga
     1501 ttgacgagca caatgccgtc ttccgcccgc ttgtacgagg cccccgtcgg acaaactgtg
     1561 acgcaggccg gcgtttcgca gtggaggcag gagcggggaa agtgcaccgt ccggctcgat
     1621 gagccctcca ccacctcata ggtgtggatg cgattgagcc aggctccgtg cggatcagcg
     1681 ccgtacgggt cctgatcggt caacggcgcc gaatagccgc cggtattcca ctccttgcag
     1741 ctcgtggcac acgcctgaca gccgacgcac gtatcgaggt cgatcacaag gccgagcttc
     1801 ttggccgtgg tttccggcaa agacgtcatt cggcggcctc tcgaaattca gcgccaaagg
     1861 tggagacgac aggccgatgc tccccgtagg ggttaggtag aggctcgaac cgcggccagc
     1921 tttcaccggc ctcctccggc ttggccctct cgatgcgcac ccgtagatcg taccaggccg
     1981 cctgccccgt gatgggatcg ctattcgaga agcgatagcc gccctcgcgc tcgggtagaa
     2041 gctccgagat tagatggttg agtaggaagc cgcgcgtggc ctcgggcgcg tctggcgcga
     2101 gaccccacgc ccctgcccgc ttgccgattg cgttccaggt ccagaccgtg tcgggattga
     2161 caccttccat cagcttcacc tggcacttca cgcgcccgat agcgctggtc acccacaccc
     2221 aatcgtcgtc cgcaacgccg agagcgcggg cgcggtcgcg atgcatgtag agccggttct
     2281 gagcagtgat ctgcctgag
//
LOCUS       DASUMG010000241         2165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_352530, whole genome shotgun sequence.
ACCESSION   DASUMG010000241 DASUMG010000000
VERSION     DASUMG010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352530"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VFX46_04030"
     CDS             complement(<1..322)
                     /locus_tag="VFX46_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX5599785.1"
                     /translation="MPALSFFKYGVAAAAFLVASLPASAADPAATPPPVTVSIITTEK
                     QDLPILEELPGRIAPMRIAEVRPRVSGIVIERVFTQGSLVKEGEVLYRIDPEPFKVQV
                     ESAKA"
     gene            complement(400..984)
                     /locus_tag="VFX46_04035"
     CDS             complement(400..984)
                     /locus_tag="VFX46_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5599786.1"
                     /translation="MGRLRIIDRDAVLTAAETVVRRDGAARLTIEAVAAEAGISKASV
                     LYDYKTKQALIKAVVERRVAAECERVRKIIEEFGAAPDSAIQGCIAAAANRSLSDADR
                     EVALCLTAALAQDPELRAPIQEKCRQQIAEILETSSRPRNALLAFLALEGLKLLECLG
                     IYTWPEDERKRLLEDISGLAAGRLAAEPGRSAQG"
     gene            1308..>2165
                     /locus_tag="VFX46_04040"
     CDS             1308..>2165
                     /locus_tag="VFX46_04040"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5599787.1"
                     /translation="MFLPIYLPVAGMALDGLMLLAMGLSVGLLSGMFGIGGGFVITPL
                     LIFLGVPPLVAVGTGVSQVVAVSVSAAITQWQAKNVDVQMGMLLLGGGIAGATLGVAL
                     QRLLKALGQIDAFISLSYVLVLGVIGTLMFMESLAAWRKRKVQPHYSRRRGGQHTWIQ
                     GLPLKRRFRTSKLYASAIPPVVIGIFVGLLTALVGVGGGFVIIPALIYLMRVPTRVAI
                     GTSVFQVVFVAAYATILHTTQNFSVDLLLALPLMIGGVLGAQYGTKIGRQLNAEQLRI
                     LLALLVLAVC"
BASE COUNT          384 a          677 c          640 g          464 t
ORIGIN      
        1 tggccttcgc gctttcgacc tgcactttga agggctccgg gtcaatccgg tagagcacct
       61 caccttcttt tacgaggcta ccctgcgtga acacccgctc gatcacgatg ccggacacac
      121 gtgggcgcac ctctgcgatc cgcatcggcg cgatgcggcc gggaagctct tccaggattg
      181 gcaggtcctg cttctcggtc gttatgatgc taacggttac cggaggcggc gtggccgcag
      241 gatccgccgc ggatgcggga aggctggcca cgagaaaggc ggcagcagcc actccatact
      301 tgaagaacga tagggcgggc attgatgtcg ttcctggcca atcaatcgat tgaggcgaag
      361 ttccgtcgcc gggcgctagc ctaattccta acgaacaact cagccttgag cgctccgccc
      421 cggttcggcc gccagcctcc cggccgcaag accggaaatg tcctccagca gccgcttacg
      481 ctcgtcctct ggccatgtgt agatgcccag acactccagg agcttcaagc cctcgagtgc
      541 caggaaggca aggagtgcgt ttcggggccg cgaagaagtt tccagaattt cggcaatttg
      601 ttggcggcat ttttcctgaa tcggtgcacg tagctccgga tcctgggcaa gtgcggcagt
      661 cagacacaac gccacctcac gatctgcgtc ggacagtgac cgatttgccg cagcagcgat
      721 acagccctgg atcgcagaat cgggagccgc accaaactcc tcgatgatct tacgcacgcg
      781 ttcgcattca gccgcgacgc gacgctcaac caccgctttg atgagcgcct gcttcgtctt
      841 gtagtcgtag aggacgctgg ccttactgat gccggcctcg gcggcaactg cctctatggt
      901 cagccgcgcc gcgccgtcac gtctgacgac cgtttcggcc gcagtcagaa cggcgtccct
      961 atcgatgatc ctgaggcggc ccatcggcca tccaatttct aaccaaacgg tcggatatat
     1021 ttcattgggc ttggcggctg tcaagcagcg ttatgccggc gcgcttagaa aactcgcgaa
     1081 cgatcagcgg gcgtcggtgt tctgcaaagg caacagcagc ggcaccgcca caccaccatt
     1141 tcgctttctg aggcggagcg ccatccgaag agaccttcgg gtctggacag cagacccgaa
     1201 aaaggccagt tgcaatcggc tggccgaggt gggaggcatg ccctgcgcgg tccacccttc
     1261 ggaccacatc accggtagct ctcgtccgtt tctgtcgtgc cgacccgatg ttcctaccga
     1321 tctatctacc cgtagccgga atggcgctcg acggcctgat gcttctggcc atgggtctct
     1381 cggtgggttt gctttcgggg atgttcggta ttggcggtgg cttcgtcata acaccgctat
     1441 tgatcttctt gggtgtcccg ccgctcgttg ccgtcggcac tggcgtaagc caggttgtag
     1501 cggtttcggt ttcggccgcg atcacacaat ggcaggcgaa gaacgtcgac gtacagatgg
     1561 gcatgctgct cctcggaggc ggcattgccg gagctacgct tggcgtcgcc cttcagcgtc
     1621 tcctcaaggc cctggggcag atcgatgcgt tcatctccct cagttacgtg ctggtgctcg
     1681 gcgtcatcgg cacgctcatg ttcatggaaa gcctggcggc atggcgtaag cggaaggtgc
     1741 aaccgcatta ttccaggcgt cgcggcggac aacacacgtg gattcaaggg cttccgctca
     1801 agcggcgctt tcgcacgtcg aagctctacg ccagcgccat tccgcctgtc gttatcggca
     1861 tattcgtcgg tctgctcacg gccctcgttg gtgtcggcgg cggcttcgtc atcataccgg
     1921 cgctcatcta cctgatgcgt gtgccgacgc gcgtggcgat cggaacgtcc gtcttccaag
     1981 tcgtcttcgt ggctgcttac gcgacgattt tacacacaac acagaatttc agtgtcgatc
     2041 tccttctcgc gctgccgctc atgatcggcg gcgtgctagg tgcgcagtac gggacgaaaa
     2101 tcggccgcca gctcaacgcc gagcagctcc ggattctact cgcattgctg gtccttgccg
     2161 tttgc
//
LOCUS       DASUMG010000242         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_513641, whole genome shotgun sequence.
ACCESSION   DASUMG010000242 DASUMG010000000
VERSION     DASUMG010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513641"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFX46_04045"
     CDS             complement(<1..572)
                     /locus_tag="VFX46_04045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX5599788.1"
                     /translation="MISIVRQGIMAAALAGSLLLSPTQYAMAQDGVVAIVNGKTITEA
                     DVRLAEAEVGSDLGSLPEATKRRVLVEYLIENQLFAEAAEGDQLGSGPEFDGRMQYWR
                     RRALRDTYFDKTVKEAVTEADAKRLYDEQVKLLKPEEEVKARHILVESEDKAKEIAEK
                     IAHGADFAEMAKQYSKDPGTKDDGGALGYF"
     gene            940..>2069
                     /locus_tag="VFX46_04050"
     CDS             940..>2069
                     /locus_tag="VFX46_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5599789.1"
                     /translation="MLSIGSIAAKIFGSANDRKLKSYRGIVAAINELEPELERLTDEE
                     LRGRTEQFRQQLKAGKSLDDLLVPAFATVREAAKRTLGQRHFDVQLMGGIALHQGKIA
                     EMKTGEGKTLVATLPVYLNALTGRGVHVVTVNDYLAKRDAEWMGEIYKFLGLTVGCIV
                     HELDDEQRRAQYACDVTYGTNNELGFDYLRDNMKMSRDEMVQRGHYFAIVDEVDSILI
                     DEARTPLIISGPVEDRAELYNAIDGFIPRLSKEDYEVDEKQRSVSLTESGNEKIEEML
                     QAAGLLKGNSLYDIENVTVVHHVNQALKAHTLFQKDRDYIVKNGQVIIIDEFTGRMMP
                     GRRYSEGLHQALEAKEKVPIQPENQTLASITFQNYFRLYDKLA"
BASE COUNT          412 a          650 c          599 g          408 t
ORIGIN      
        1 ctgaaatagc ccaacgcccc gccgtcgtcc ttggtgccag ggtccttgga gtactgcttg
       61 gccatctcgg caaaatcggc cccgtgcgcg attttttcgg cgatctcttt cgccttatcc
      121 tcgctctcca ccaggatgtg acgcgccttg acctcctcct cgggcttcag cagcttaacc
      181 tgctcatcat agagccgctt ggcgtcggct tccgtcaccg cctccttgac ggtcttgtca
      241 aaataggtgt cgcgtagcgc tcggcggcgc caatactgca ttcgcccgtc gaactcgggg
      301 ccggagccga gctgatcgcc ctcggccgcc tcggcaaaca gctggttctc aatcagatac
      361 tcgacaagca cccggcgctt ggtagcttct ggcaggctgc cgagatcaga gccgacctcg
      421 gcttcagcca gtcggacgtc ggcttccgta atggttttgc cattcacgat cgccacgacc
      481 ccatcttggg ccatggcgta ttgggtaggg gagagcaaga gactaccggc tagtgcggca
      541 gccatgatgc cctgtctcac tatcgagatc accggagtgt gttccttgaa tagtggggcg
      601 ggcagccacg aagtctaacg cgaaccgatc gccgcacggg ttaaagataa taatctgtct
      661 cgatggttag accgcattcg ccggtttgcc aagccgcgtt cgccgccgcg tttgcaatcc
      721 ggccagaacg gatgcatggc gaactgtcgc gttcgattga ttgacatttg ccggtccggc
      781 tccttatctc tcgcctatcg agtggcattc tccgtccgcc ggtagcccgc tcgcgcctgt
      841 ttgcgcttcc accccggcca atttcgtgtt ccttctcctg gcgcgaacac gtgtgaccgg
      901 gtctcaaggc gctggacatg cctgcaagga taggtgcaca tgctttcaat cggctcgatc
      961 gcggccaaga ttttcggttc agccaatgac cgcaagctca agtcctatcg cggaatcgtg
     1021 gctgctatca atgagttgga gcccgagctc gaacggctga ccgacgagga gcttcgcggc
     1081 cgcaccgagc agtttcgcca gcaactcaag gccggcaaga gcctggacga tctcctcgtc
     1141 ccggcgttcg caaccgtgcg cgaagcagcc aaacgcacgc tggggcagcg ccacttcgac
     1201 gtgcagctga tgggcggcat tgccctacac cagggcaaaa tcgcggagat gaagaccggc
     1261 gagggtaaga cgctggtcgc tacattgccc gtttacttga atgccctcac tgggcgcggc
     1321 gtccacgtgg tgacggtcaa cgattatctg gccaagcgcg acgccgaatg gatgggcgag
     1381 atctataagt ttctcggcct gacggtcggc tgcatcgtcc acgagctcga tgacgagcag
     1441 cggcgagccc agtacgcgtg cgacgtcacc tatggcacga acaacgagct gggcttcgac
     1501 tatctccgcg acaacatgaa aatgagccgc gacgagatgg tgcagcgcgg ccattacttc
     1561 gccatcgtcg acgaggtgga ctcgatcctc atcgacgagg cgcgcacgcc gctcatcatc
     1621 tcgggaccgg tcgaggaccg cgcggagctc tacaacgcca tcgatggttt cattccgcga
     1681 ctgtcgaagg aagactacga ggttgacgag aagcagcgct cggtctcact caccgagtcg
     1741 ggcaacgaga agatcgagga aatgctgcag gctgcgggcc tgctcaaggg caactcgctc
     1801 tacgacatcg agaacgtcac ggtcgtccat cacgtcaatc aggcgctgaa agcacacacg
     1861 ctcttccaga aggaccgcga ctatatcgtc aagaacggcc aggtcatcat catcgatgag
     1921 ttcaccggcc gcatgatgcc gggccggcgc tactccgagg gcttgcacca ggcgttggaa
     1981 gccaaggaaa aggtgccgat ccagcccgag aaccagacgc tggcctcgat caccttccag
     2041 aactacttcc gcctctacga caagctggc
//
LOCUS       DASUMG010000243         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_352658, whole genome shotgun sequence.
ACCESSION   DASUMG010000243 DASUMG010000000
VERSION     DASUMG010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352658"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..706)
                     /locus_tag="VFX46_04055"
     CDS             complement(59..706)
                     /locus_tag="VFX46_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599790.1"
                     /translation="MLLWRRLLVLFSLAMLMALADKAHAGKFEFRVTGVKRSDVLNIR
                     ERVDDQATISQKKILGQIPANSGGIYGSGATLRVGSSRWYEIRYKNVRGWVNGRYLQP
                     VSSDLGSELESNLFCGGTEPFWALKVERNTAELSRPDGPPVRYTVAIREPFQGRKDAL
                     ALRLVSDAAPAISALVQHKEWCSDGMSDLEYAFEVRIIGLKTGEVPLHGCCSLLR"
     gene            complement(1109..1912)
                     /locus_tag="VFX46_04060"
     CDS             complement(1109..1912)
                     /locus_tag="VFX46_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599791.1"
                     /translation="MLRVGGLLVLCLMVLALPAQAQRGKHNDLVLLGEKSVGFLVDRD
                     VIEVGKSEDWFRDRAFRSLHFKAERNDLHLISIRLVYLNGYKEDLKVDRTIRAGRQLP
                     VDLRGERSYLRRIEMIYRSRPSFRGQAVIKVYGEPAPRSIRPPRFEPPKNWELLGEKS
                     VGFRVDRDVINVKHPENWFRDRGFRMLHFVVEDNEVHLISIRIVYFNKYTETIKVNRD
                     VRPGGHVAVDLRGDRSYLRQIEMVYRAEPSRRGNATVKVYGEPAPRGRR"
     gene            complement(2132..2362)
                     /locus_tag="VFX46_04065"
     CDS             complement(2132..2362)
                     /locus_tag="VFX46_04065"
                     /inference="COORDINATES: protein motif:HMM:NF018294.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HEX5599792.1"
                     /translation="MMAASRSTYFVSPRQRLVSRLLQAVREAKRAYVRWYNRRAACHY
                     LSSLDDRMLRDIGISRSQIEAAVRNGRPSQQA"
     gene            2510..>2868
                     /locus_tag="VFX46_04070"
     CDS             2510..>2868
                     /locus_tag="VFX46_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX5599793.1"
                     /translation="MRRADRLFEIIQLMRRKAPVRARDLSEALEVSERTIYRDIQDLI
                     ASGVPIEGEAGVGYVLKAGFDLPPLMFKEQEIEALVLGARIVESLPDAELAEAASNAI
                     AKIEAVIPDRLRGYMANT"
BASE COUNT          612 a          872 c          818 g          566 t
ORIGIN      
        1 caggtagagg ctgagctgcg ctgcttctga tttccgacac gccgatcgaa gcgcaagctc
       61 acctcaacaa gctgcagcaa ccgtgcagcg gaacctcccc ggtcttcaga ccgataatgc
      121 gcacctcgaa agcgtactcg aggtccgaca tcccgtcgct gcaccactcc ttgtgctgca
      181 cgagagcact gatggccggg gccgcatcgc tgacgaggcg tagggcgaga gcatccttac
      241 gcccttggaa cggctcgcgt atggcaaccg tgtaacggac cggcgggccg tctgggcgac
      301 tgagctcggc cgtgttccgc tccaccttca gcgcccagaa tggctcggtc cccccacaga
      361 acaaattgga ctccaactcg ctgccaaggt ccgaggagac gggttgcaga tagcgcccgt
      421 tgacccagcc gcgaacattc ttgtagcgga tctcgtacca gcgggaagag ccgacccgga
      481 gagttgctcc agatccgtaa atgccgccgc tgttggccgg aatttgcccc agaatctttt
      541 tctggctgat tgtggcctga tcatccacgc gctcgcgaat gttgagcaca tcagaccgct
      601 tcacaccagt cactcggaat tcgaatttgc cagcgtgcgc cttatcggcc aacgccatca
      661 gcatggcgag gctgaataga acaagcaaac gtctccaaag cagcatcgta taggagggct
      721 cctcggcaaa gctggcactg atagctaagc tcagccatct taatatcagc cgttgcaaat
      781 gccagcttcg cccgggccga ggcaagtcga atacaacttg gagatttgat ttgcaggcag
      841 gccctcgtcc ctttggttta aattcgcctt atcggcttct caaacgagga cgacaaattt
      901 gatccgggtg aggcaatgct gatgggcacc cagtgacggg cgtcaccagg tgcccattcc
      961 ggggcctgtg cttggtaggg ggcgtccggc acaggcaagg tgccggaaga accaacgcca
     1021 agcgagttgg ttccaattca aattcggcag tcaaaacagg cgacagcata aattgtgcgc
     1081 tgtcgcccgc tacgcgctca agctgcggtt agcgccgccc cctcggggcg ggctcgccgt
     1141 agaccttgac ggttgcattg cctcgccggc tcggctcagc ccggtaaacc atttcgatct
     1201 ggcgcagata gcttcggtcg ccccgaagat cgaccgcaac gtggccgcca ggacgaacat
     1261 ctcggttgac cttgatggtt tccgtgtact tgttgaagta cacgatgcgg atcgagatca
     1321 gatggacctc gttgtcctcg accacgaagt gaagcatccg gaatccgcgg tcacggaacc
     1381 agttttccgg atgtttcacg ttgatgacgt cacggtccac ccggaacccg acagacttct
     1441 caccaagaag ctcccaattc ttgggcggct cgaatcgagg cgggcgaata gaccggggcg
     1501 caggctcacc atagaccttg atgaccgcct gtccgcggaa gctggggcgg gagcgataga
     1561 tcatctctat gcgccgcaga tagcttctct caccgcggag atcgaccggc agctgacgac
     1621 ccgcacggat cgtgcgatca accttcagat cttctttata gccgttcagg taaacgagcc
     1681 gaatcgaaat caggtgcaga tcattgcgct ccgccttgaa gtgcaggctc cggaacgcgc
     1741 gatcgcggaa ccagtcttcc gatttgccca cctcgataac atcgcgatca accaggaagc
     1801 cgacgctctt ttccccgagc agcaccaagt cgttgtgttt gcctctctgg gcttgggcag
     1861 gcaaggcaag caccatcagg cagagcacaa gtaaaccccc aacccgcagc atttgtcctc
     1921 cgtagcagga ttaaccaaat gtaagccgct aactcttgcc tacggcggcg ccgaagtaaa
     1981 cgaaaatcgc tgtgcccgga ctattcaggc cttctgatgg cggaaatgcg gctgaaatcg
     2041 catctatgac tagcacggcg caagtagaac gtcttaccaa ccggcattga aagttggcga
     2101 gggctctcgg cgactaggtc ttgatccgtg attacgcctg ctgactgggg cgaccgttgc
     2161 gaactgccgc ctcgatctgg cttcgcgata tgccgatgtc tcgcagcatg cggtcatcca
     2221 ggctgcttaa gtagtggcac gcagcacgcc ggttgtacca cctgacatag gctcgcttcg
     2281 cctcacgaac cgcctgcaat aagcggctga ccagacgttg tcgcggcgaa acgaaatacg
     2341 tgcttcggga tgctgccatc atgtgctcct gtcatctcgt tctccagtcg cactatggaa
     2401 gacagctcct gacagcctgg cgtcagcagc tccgacacgc tcctgacacc gcggtgtcag
     2461 gagtgatagg ttacgtatct tagcgcaagc tagatcgtgg attggcagcg tgcgccgcgc
     2521 cgaccgactg ttcgaaatca tacagctgat gcgccgcaag gcccctgtgc gcgctcgcga
     2581 tctgagtgaa gcgctggagg tctcggaacg gaccatctat cgcgacattc aggacttgat
     2641 cgcaagcggc gttcccatcg aaggggaggc cggtgtcggc tacgtgctca aagccggctt
     2701 cgatctccca ccgctgatgt tcaaggagca ggagatcgaa gccctcgtcc tgggcgcacg
     2761 catcgtcgag tccctgcccg atgcggagct ggccgaagca gcctcgaacg cgattgccaa
     2821 gatcgaggca gtcattccgg accggttgcg cggctacatg gccaatac
//
LOCUS       DASUMG010000244         2334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1156327, whole genome shotgun sequence.
ACCESSION   DASUMG010000244 DASUMG010000000
VERSION     DASUMG010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156327"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1585)
                     /locus_tag="VFX46_04075"
     CDS             complement(<1..1585)
                     /locus_tag="VFX46_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5599794.1"
                     /translation="MNEQLRRTSAREEFAGAGDPGALEALFGARRGFASALSPAEAAL
                     VQAYRDRGFLRSRLDPLGLAPIPTVPELDPARYGLDPAKARSLVKELERVYCGSIGWE
                     FGHIHDTAKRQWLELQAEQGALPGTAERQWMLALLTRAHTFERVLAQRLPTARLFGLG
                     GAESFIALIEVLLTESVPLGLEEVVIGGMHRGRFNLLANVCGKPLGALLAEIQGKPPV
                     PEGLGVSSDVPYHLGYSGEREITGRNIRFSVSPHPSHLQLVPIISQGRARAKQRARGV
                     PEGQRAVLPLLLHTDASFAGQGLVAEMFQLSRLAPYDLGGTIHVVINNQIGFTTLPEE
                     GRSARHPTDIAKLVEAPVLHVNGDDPDAVYQVAIVAAHWRATFASDIVIDLVCYRRPG
                     HNEFDEPRFTQPLMYRAIDARPPVNEIYAARLEAEGIDTSLMAAEADTMSERIAAGLA
                     AAKSYSVNRADWFEGAWKGFRAGSTVEMLGSPETGLPVDELRRLGRLLTEVPAGFSLD
                     PKVARFLEERRHTIETGEGL"
     gene            1701..>2334
                     /locus_tag="VFX46_04080"
     CDS             1701..>2334
                     /locus_tag="VFX46_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX5599795.1"
                     /translation="MTAPLSHIRVLDLSRVLAGPLATQFLADMGAEVIKIERPRSGDD
                     TRAWGPPFAVEPTENDPGISAYFACCNRGKQSVTVDITHPKGQELIRKLAAKSDVVIE
                     NFKVGGLAKYGLDYQSLKQINPRIIYCSITGFGQTGPYAARAGYDFLVQAMGGLMSIT
                     GEPDDVPGGGPVKVGVAISDQMAGMFALSAILTALLKRERTGEGEYIDVAL"
BASE COUNT          428 a          763 c          728 g          415 t
ORIGIN      
        1 tgaggccctc gccagtttcg atcgtgtgcc gccgctcctc caggaaacgc gccaccttag
       61 gatcaagaga gaacccggcc ggaacttcgg tcagcaacct gcccaggcgc ctcagctcat
      121 ccacgggcag ccctgtttcg ggcgagccca gcatctcgac cgtgctgcca gcgcgaaagc
      181 ctttccatgc gccttcgaac caatcggcgc gattgacgga gtaggacttt gctgccgcaa
      241 gccccgctgc gatcctttcg ctcatcgtgt cggcctcggc ggccatgagc gatgtgtcga
      301 tcccctccgc ctcgagccgc gccgcgtaga tctcgttcac cggcggacgg gcatcgatcg
      361 cgcgatacat cagcggctgc gtgaaccgcg gctcgtcgaa ttcattgtgg ccagggcggc
      421 gatagcacac gaggtcgatg acgatgtcgc tggcaaatgt cgcgcgccaa tgtgctgcca
      481 cgatggcgac ttgatagacg gcatccggat catcgccgtt cacgtggaga accggcgcct
      541 cgaccagctt cgcgatgtcg gtcggatgcc gcgcggaacg cccttcctcc ggcagcgtcg
      601 tgaagccgat ctgattgttg atgacgacat ggatcgtgcc gccgagatcg tagggcgcca
      661 accgcgaaag ctggaacatt tcggccacga gcccctgccc tgcgaagctc gcatccgtgt
      721 gcaacaagag cggcagcacg gcgcgctgcc cttccggtac accgcgcgcc cgctgcttgg
      781 ctcgcgcccg gccctggctg ataataggca cgagttgtag gtgcgaggga tgcggagaaa
      841 cggaaaagcg gatgttgcgg ccggtaatct cccgctctcc ggaatagccg aggtggtagg
      901 gaacgtctga cgacactcca agcccttcgg gcactggggg cttaccttga atttcggcca
      961 gcagcgcgcc gagcggcttg ccgcacacgt tggcgagcag attgaaccgc ccgcgatgca
     1021 tgccgccgat gaccacctcc tcgaggccca gcggcacgct ctcggtcagg agcacctcga
     1081 tcaaggcaat gaagctttct gcgccgccga gaccgaagag tcgcgcggtc gggagcctct
     1141 gcgccagtac gcgctcgaac gtatgcgccc gcgtgagcag cgccagcatc cattggcgtt
     1201 ccgcagtgcc tggcaatgcg ccctgctcgg cttgcaattc gagccactgc cgcttggccg
     1261 tgtcgtggat gtgcccgaac tcccatccga tcgatccgca gtagacgcgc tcaagctcct
     1321 tgacgagtga gcgcgctttg gccggatcga gcccataacg ggcgggatcg agctccggaa
     1381 cggtcggaat cggcgcgagc cccagcggat cgaggcggct tctcaagaat ccgcggtcgc
     1441 ggtaggcctg caccagcgcc gcctcggcag gcgacaatgc ggaagcgaaa ccgcgcctgg
     1501 ctccgaaaag cgcttcaagg gcaccaggat cgccggctcc agcgaactcc tccctcgccg
     1561 aggtgcggcg tagctgctca ttcattccgg ctgcaatccc tgggaagtct gtgtcctctc
     1621 cagcaggatg acaccatgac acctgctggg cgctttcgtg ctaacggtta gcgcacaatc
     1681 cactgccggg aaacgtctca atgactgctc cgctttccca catccgcgtg cttgacctgt
     1741 cgagggttct cgccggcccg ttggccacgc agttcctggc cgacatgggt gccgaggtga
     1801 tcaagatcga acgtccacgg agtggcgatg acactcgcgc ctggggccca cccttcgccg
     1861 tggagccgac cgaaaacgac ccaggcatct cggcgtactt cgcctgctgc aatcgcggca
     1921 agcagtcggt gacggtggac atcacgcatc ccaaggggca ggagctcatc cgcaagcttg
     1981 ccgccaagtc ggacgttgtg atcgagaact tcaaggtcgg cgggctcgcc aaatatggtc
     2041 tcgattacca gagcctgaag cagatcaatc cccggatcat ctattgctcc atcactggct
     2101 tcggccagac cgggccatat gcagcacggg ccggatacga tttcctcgtt caggcgatgg
     2161 gagggctgat gagcattacg ggcgagcccg atgatgtgcc tgggggaggc ccggtgaagg
     2221 tcggcgtggc catttcggat caaatggcag ggatgttcgc cttatccgcg atcctgaccg
     2281 cgcttctcaa acgcgagcgc accggcgaag gcgagtacat tgacgtcgcc ctgc
//
LOCUS       DASUMG010000245         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_834663, whole genome shotgun sequence.
ACCESSION   DASUMG010000245 DASUMG010000000
VERSION     DASUMG010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834663"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VFX46_04085"
     CDS             <1..1036
                     /locus_tag="VFX46_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599796.1"
                     /translation="EAKTWWQGDRIWTTSLDDTFLGGKLLPDALASAAEDYTGERAMG
                     AAEPLGTQGWQALADLRAWPRAVAPIIETPKKFVRLRSGHNVVLKFAGMDLTGQQFWE
                     SQANARLVIDRAAVSPLGCHQGWLVLPWIDGERLSAADADSGFITEYLGPWIAAVSSR
                     KLHPDEIRAGIRRVADALSVWATRQDAFPVSSIEGMTEQVLGEVGTASQPCYGDGRLA
                     PHEWIREPSGTIRAVDFGAHDRDHTWVGPQPIVWDLVGAEVEWGLDPVRAGELRSNVQ
                     SLTGYSCSERTRAFYIAGYCAFRAAAAHYSATMKNEAALRDHLLEAERYYEQRLKSTL
                     AFLTTDVRAA"
     gene            complement(1139..3187)
                     /locus_tag="VFX46_04090"
     CDS             complement(1139..3187)
                     /locus_tag="VFX46_04090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX5599797.1"
                     /translation="MSASSAANPLLVPWPTPFGVPPFSEIKPEHFRPAFEQALAEHCA
                     EIDRIAKDPAGPTFENTIAALENSGRTLDRVSCVFWNLASAHTNDELQAIEREIAPKL
                     AQHWNAITSNTALFARIDAVMHSAETAGLDAEQKRVLERTHLAFVRAGAKLAPEEKKR
                     LGEILERLATLGTQFSQNVLADEKSYELPLSEEDLVGLPEFLIQAARKAAEERGSAAK
                     YVITLSRSLIEPFLAFSPRRDLREQAFKAWISRGENGGASDNRAIIAEMLKLRRERAH
                     LLGYKTFADYKLENTMAKTTANVRALLNEVWPAAKARALQEREKLQAIAQREGANITI
                     EPWDWRYYAERVRKAEYDLDESEVKPYLPLDNVIEAAFYVAGRLFGLSFSERPDVPVY
                     HPDVRAFEVTDSAGNHVGLFLGDYFARPSKRSGAWKSTFRRQERLAGDIRPIVVNVMN
                     FAKGSGDTPALLSFDDARTLFHEFGHALHSLLSNVTYPSISGTSVARDFVELPSQLYE
                     HWLLTPEVLRKFARHYRTDEPMPAHMIEKLRAASTFNQGFATVEYLSSAIVDIEFHSE
                     LYDDQEPIAFEQSVLERIGMPREIVMRHRSPHFLHIFSGDGYSAGYYSYLWSEVLDAD
                     AFAAFEETGDVFDPDTAARLKEFIYSAGGLRKEEEAYKAFRGRLPTVEGLLKKRGLIA
                     "
     gene            3434..3838
                     /locus_tag="VFX46_04095"
     CDS             3434..3838
                     /locus_tag="VFX46_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599798.1"
                     /translation="MRKWIFAVLAVAGTALIATPSAMAQSEWGTEEDDQIWDEFGEPE
                     YFSPPMGTQLYGYRTLGVVTPPGVTPQTRVYRYSAPAPAPAPGLSYFSPPRTAPGGTV
                     ETEVQVGPGPGECGTNFYWSEAAGRCVDARMR"
BASE COUNT          773 a         1112 c         1165 g          789 t
ORIGIN      
        1 ggaggcaaag acgtggtggc agggagatcg catctggacg accagtcttg acgatacgtt
       61 tctaggcgga aaattgctgc cggatgcgct cgcctctgcg gcggaagact atacgggcga
      121 gcgggcaatg ggtgcggcag agccgctcgg cacgcagggt tggcaagctc ttgccgatct
      181 ccgagcgtgg ccacgggcag ttgccccgat catcgagacg ccgaagaagt tcgttcgtct
      241 gcgttcgggc cacaatgtcg tcctgaaatt cgccgggatg gacttaaccg gtcagcaatt
      301 ttgggaaagt caagcaaacg cgcgccttgt gatcgatcgc gcggcagtat ctcctctagg
      361 gtgccatcag ggctggcttg ttctcccctg gatagacggt gagcggctga gcgccgcaga
      421 cgctgattcc ggattcatca cagaatatct tggtccttgg attgcagcgg tttcatctag
      481 gaagcttcat ccggatgaaa ttcgggccgg aattcgccga gtcgctgatg cgctttcggt
      541 gtgggccacg aggcaagatg cctttcctgt ttcttcgatc gagggcatga ctgagcaggt
      601 gctcggcgag gtcggcacag cgtctcagcc ctgctatggc gatggacgct tggcgcctca
      661 tgaatggatt cgcgagccaa gcggcactat ccgggcagtt gattttgggg cgcatgatcg
      721 cgaccatacc tgggtcggac cccagcctat tgtctgggat ttggtggggg cggaggtcga
      781 atgggggctc gaccctgtgc gcgctggcga gcttcgttcc aacgttcaga gcctcactgg
      841 ctattcgtgc tctgaaagaa cgcgagcttt ttacattgct ggatactgcg ccttcagggc
      901 ggccgcagct cattactcag caacaatgaa aaatgaagct gcactccgcg atcatttatt
      961 agaagctgaa cgttattacg aacagcgact gaaatcgacg ttagcttttc tgacgactga
     1021 cgtgcgagct gcgtgagtgg ccgccggcta taacgcattt gccgaccatc ggtggaaatg
     1081 cttcacactc agcagggtaa tcctgctaag tcctccctat ccgcctgcgt tactgcgcct
     1141 acgcgataag tccgcgcttt ttcagcagac cttcgacggt cggcagccgg ccgcggaagg
     1201 ccttgtaggc ttcctcttcc ttcctcaagc cgccggccga gtagatgaac tccttcagcc
     1261 gcgcggctgt atcgggatcg aaaacatcgc ctgtttcctc gaaagcggca aatgcatccg
     1321 cgtccagcac ttcggaccac aggtagctgt aatagccggc cgaatagcca tcgccggaga
     1381 aaatgtgcaa gaagtgcggt gaacggtgcc gcatcacgat ttcgcgcggc atgccgatgc
     1441 gttcgagcac ggactgctcg aaagcgatgg gctcctgatc gtcgtaaagc tccgagtgga
     1501 attcgatatc gacgatggca gagctcaggt attccaccgt cgcaaaaccc tggttgaagg
     1561 tgcttgccgc cctcagcttc tcgatcatgt gtgccggcat cggctcatcg gtgcggtaat
     1621 gccgggcaaa cttgcgcaaa acctcgggtg tcagcaacca atgctcgtag agctgtgacg
     1681 gcaactcgac aaaatcgcga gcgaccgagg tgccggagat cgatggatag gtcacgttcg
     1741 acaacagact gtgcaacgcg tggccgaact cgtgaaacaa cgtgcgcgcg tcgtcgaacg
     1801 acaacagggc cggcgtgtct cccgagcctt ttgcgaaatt catcacattg accacgatcg
     1861 gccggatgtc gcccgcgagc cgctcctgac ggcggaaggt gcttttccaa gcgcccgatc
     1921 gctttgaagg ccgcgcgaag tagtcgccaa ggaagaggcc gacgtgattt cccgcgctgt
     1981 cggtcacctc gaacgcgcgt acatcagggt gatagaccgg cacgtctggg cgctcgctga
     2041 agctcagccc aaacagccgt cccgcgacgt agaatgcggc ctcgatgaca ttgtcgagcg
     2101 gcaagtacgg cttcacctca gactcatcga ggtcatactc ggccttccgc actctttcgg
     2161 catagtatcg ccaatcccac ggctcgatgg tgatattggc gccttcgcgc tgagcgatgg
     2221 cttgcagctt ttcgcgctct tgcagcgccc gcgccttcgc cgcaggccag acctcgttca
     2281 ggagtgcccg aacgttcgcg gtcgtcttgg ccattgtgtt ttcgagcttg tagtcggcga
     2341 atgtcttata cccgagcagg tgagcgcgct cgcgccgcag cttcagcatt tcggcgatga
     2401 tggcccggtt atctgaagcg ccgccgttct cgccgcggct gatccaggcc ttgaacgcct
     2461 gctcccgcag gtctcggcgg ggagagaaag cgaggaatgg ctcgataagg gagcgcgaaa
     2521 gagtgatcac gtacttcgcc gcgctgccgc gctcctcggc agctttccgc gcggcctgaa
     2581 tgagaaactc gggcaagcca accagatcct cctcgctgag cggcagctcg tagctcttct
     2641 catccgcgag gacattctgg ctgaactgcg tgcctaacgt tgccagccgc tcaaggattt
     2701 cgccaagtcg cttcttctcc tcaggcgcaa gcttggcgcc agcccgcacg aacgcgagat
     2761 gagtgcgctc taagacgcgc ttctgttcag catcgagccc agccgtttcc gcgctatgca
     2821 tcacagcgtc gatgcgcgca aaaagagctg tattcgatgt gatagcattc caatgctgcg
     2881 cgagcttcgg cgcgatttcg cgctcgatgg cttgcagctc atcgtttgtg tgggcgctcg
     2941 ctaggttcca gaaaacgcaa gagacgcggt ccagcgtccg gccggaattc tccagcgctg
     3001 caatagtgtt ctcgaaagtc ggccctgcgg ggtccttcgc aatgcggtcg atctcagcgc
     3061 aatgttcggc aagagcttgc tcgaaggctg gtcggaaatg ctccggcttg atttcggaga
     3121 agggcggcac gccaaacggc gtgggccatg ggacgagcaa aggatttgca gccgaactgg
     3181 cgctcatcag atggaaacct cgggggaagg ctggaggatg gcgaaaccta gaaacgatat
     3241 agtctagaag gatctgcaac gtaaagtgga agctagcaaa ccaaatctgc tcgcatcctt
     3301 tccattcagt taggcttagg gcctgtcgag ccctcctgag ccagcaggct acggccagcc
     3361 acctgcattt tgccgcacgg aaccagtccc aaatcgcatc gttgacaggc gactcaaaaa
     3421 ggaggtcgct cagatgcgga aatggatttt tgctgtactg gccgtcgccg gcacggcgct
     3481 gattgcgacg cccagcgcga tggcgcaaag cgaatggggc accgaagaag atgaccagat
     3541 ttgggatgag ttcggtgagc ccgagtactt cagtccgccg atgggaacac agctctatgg
     3601 gtatcgcacc cttggcgttg tgacaccgcc tggcgtgaca ccgcagactc gggtctatcg
     3661 ttactcagcc ccagctccgg ctccagcccc aggtctctcc tacttcagtc cgccacgaac
     3721 ggcaccagga gggacggtcg agactgaagt gcaggtcggc ccgggaccgg gagaatgcgg
     3781 caccaacttc tactggtctg aagctgctgg gcggtgcgtc gacgcccgca tgaggtaac
//
LOCUS       DASUMG010000246         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_272650, whole genome shotgun sequence.
ACCESSION   DASUMG010000246 DASUMG010000000
VERSION     DASUMG010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272650"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1974)
                     /locus_tag="VFX46_04100"
     CDS             complement(<1..1974)
                     /locus_tag="VFX46_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HEX5599799.1"
                     /translation="MARFPVDKARLNRFAGRILARYVDFVYRTSRVVGEPADTAAFVR
                     AHSPFILAMWHGQFLMLPRVERGGLKVHSMVARHGDAELIGQVLEHFGMDMIRGAGAG
                     TRRRNRGGAYALRAALRVLANGDCVAMTADVPPGPARVAGQGIVTLARLSGRPIIPAA
                     TATSRYKAFQTWSRMTLNLPFGTLAVVAGEPIYVDPDEDEEGLEKARLKVQEALNAVT
                     ARAYELAGADPARATPPPVGRAAPPIPPGVLYKSYRALTGLARPAVSLLLDARTRQGK
                     EDRQRRGERFGQASVARPEGCLVWVHAASVGETNAILPLLTALRARRPDIRFLLTTGT
                     STSAAIASSRLESHDIHQYVPLDVPKAVRDFLDHWCPQLAIFTESEIWPNLIMETSAR
                     SIPLALVNARMSSRSYARWRRWRGLAKPMFSRFDVVLAQNEKLARQFGYLGARNVLAA
                     GNLKIDAPPPAADAAEVNRLRGLLDGRPCLVAASTHEGEEEIIGLAHKIIAQRAASLC
                     TIVVPRHPERGGRIAEILREQGLEVSRRSQGAVPERSTDVYIADTLGELGTFYSLTPL
                     AFIGGSLIPRGGQNPVEAIRHGAVVITGPHWANFSDAYRALFRRRAALQVDSAESLAR
                     AVGSLMDDEVELARMRASAQAALAGLSGALNRTV"
BASE COUNT          419 a          746 c          719 g          398 t
ORIGIN      
        1 cacggtgcgg ttcaatgcgc ctgacagccc tgccaatgct gcttgagcgc tggcccgcat
       61 ccgcgccaat tccacttcat cgtccattag cgacccgacg gcgcgagcca ggctctctgc
      121 cgaatcgacc tgtagggcgg cgcggcggcg gaaaagagca cgataggcgt cgctgaaatt
      181 ggcccagtgg ggaccagtga tcaccacagc accgtgccga atggcctcca ctggattttg
      241 tccgcctcga gggatcagcg agccaccaat gaaggccaga ggtgtaaggc tgtagaacgt
      301 gccgagctca cccaaggtat ctgctatgta gacatccgtg ctccgttcag gcacggcacc
      361 ttgcgagcgt cgtgagacct cgaggccctg ctcccgcaga atctccgcga tgcggccgcc
      421 gcgctccgga tggcgaggca ccacgatcgt gcacaagctc gccgctctct gcgcgattat
      481 cttgtgcgcc agaccgatga tttcctcctc cccttcgtgc gtgctcgccg caaccaggca
      541 aggccgtccg tcaagcaatc cccgtaagcg attgacctcc gccgcatcgg ccgccggggg
      601 cggtgcgtcg atcttcagat tgccggcagc taggacgttg cgtgctccca agtatccgaa
      661 ctggcgggcc agcttctcgt tttgggcgag cacgacgtcg aaccgactga acatcggctt
      721 cgccagtccc cgccatcgcc gccaccgtgc ataagaccgg ctcgacatcc gggcattaac
      781 gagcgcaagg gggatcgagc gggccgacgt ttccatgatc aagttcggcc agatctccga
      841 ttcggtgaaa atcgcgagct gcgggcacca atgatcgagg aaatcccgca cggccttcgg
      901 cacgtcgagt gggacatact ggtggatgtc gtgcgactcg aggcggctcg atgctatggc
      961 cgccgaggtc gaggttccgg tcgtgaggag aaagcggatg tccggccgcc tggccctgag
     1021 cgccgtcaag agcggcagaa tggcgttcgt ctcgccgacg ctcgccgcgt ggacccaaac
     1081 caggcagcct tcaggccggg ctacgctggc ctgcccgaag cgttcgccgc ggcgctgccg
     1141 gtcctccttg ccttgccgcg ttcgagcgtc gagcaacagg gaaacggctg ggcgtgcaag
     1201 tcctgtcagg gcgcggtagg atttgtacaa gacaccaggc ggaatcggcg gggcggctct
     1261 cccgacgggg ggtggtgtag cccgagccgg atcagcaccg gccagctcgt atgctcgtgc
     1321 ggtgacggcg ttgagcgcct cctgaacctt gagacgcgcc ttctccaacc cctcctcatc
     1381 ctcatccggg tcgacataga tcggctcgcc tgccacgaca gcgagcgtcc cgaaaggcag
     1441 gttgagcgtc atccgactcc aggtctggaa cgccttgtag cggctggtgg ccgtcgctgc
     1501 gggtatgatc gggcgcccag acagacgcgc cagtgtcacg ataccttgac cagcgacccg
     1561 tgccggccct gggggcacat ccgccgtcat ggcgacgcaa tcgccgttcg ccagaacgcg
     1621 caatgccgca cgaagtgcat aagcgccacc gcggtttcgt cgccgcgtcc cggcgccagc
     1681 gcccctgatc atgtccatcc cgaaatgctc gagaacttgt ccgatcagct ccgcgtcgcc
     1741 gtggcgcgca accatgctgt gcaccttcag cccgccgcgt tccacccgcg ggagcatgag
     1801 gaactgcccg tgccacatag ccaggatgaa ggggctgtgc gctcgcacga atgccgccgt
     1861 atcagcgggc tcaccgacga cgcgcgacgt acgatagacg aagtccacgt accgcgcgag
     1921 gatgcggccg gcgaaccggt tgaggcgagc cttgtcgaca ggaaatcgag ccaccctctg
     1981 gcgtgccttt tggcaaactt gggaatcggt atgcgcagcc cgtccccttg attggggtgt
     2041 ctatagcgga taagcaccct ttgcaattgc gaatctgagc gattgctgat cggagagact
     2101 ggcgctgatc ggttgccgcc agcaggagtt ccacagaagc agacgaatgg cgaacgtagg
     2161 cctagggaaa cgagcccggc ggcaattcaa tcggcttgtc aaccgatgcc gaaatgcgct
     2221 cgtcgagcat agtcctgatt gggccaaggc tgccttcggc cctgccgcca gctacttcga
     2281 ca
//
LOCUS       DASUMG010000247         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1156688, whole genome shotgun sequence.
ACCESSION   DASUMG010000247 DASUMG010000000
VERSION     DASUMG010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156688"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VFX46_04105"
     CDS             complement(<1..289)
                     /locus_tag="VFX46_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEX5599800.1"
                     /translation="MTNNDQIKDEDSLIQSYFAPLTVGFPGAYGLEEDCAALTPEAGH
                     DLVLKTDAIAEGIHFRPEDAPEDVAWKALAVNVSDLAAKAARPVGYLLSLAF"
     gene            complement(402..899)
                     /gene="nusB"
                     /locus_tag="VFX46_04110"
     CDS             complement(402..899)
                     /gene="nusB"
                     /locus_tag="VFX46_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX5599801.1"
                     /translation="MTPENKPERRSARSAARVAAVQGLYQMDLAQTDLNAVIEEFQTL
                     RFPQAEPGDAIAGADPTFFAELLRGVVRRQRDVDPLIDQQLAAGWRLARVDSILRAIL
                     RSGVYELLERQDVPARVVINEYIDIAHDFFSEDEPRVVNGVLDKIARRLRPSELDAKP
                     GRRGS"
     gene            complement(896..1372)
                     /gene="ribH"
                     /locus_tag="VFX46_04115"
     CDS             complement(896..1372)
                     /gene="ribH"
                     /locus_tag="VFX46_04115"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX5599802.1"
                     /translation="MPARLRYRILIIEGRFYDHISDALLEGAVAELEAAGATYDRVTV
                     PGALEIPQALMQAVAAGLIPATAPSAWYSGAVALGCVIRGETSHYDIVCNNANHWLME
                     VAIRHNVPVGNGILTVDTEAQALSRAEGGRDGKGGAAARACLQLLDIARQFQGQGA"
     gene            complement(1424..2584)
                     /gene="ribB"
                     /locus_tag="VFX46_04120"
     CDS             complement(1424..2584)
                     /gene="ribB"
                     /locus_tag="VFX46_04120"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX5599803.1"
                     /translation="MNSSLKTVSYSEGRGLLSSTPELLEDLRNGRMVILVDDENRENE
                     GDLVIAAQMATPDAINFMAKHGRGLICLALTKARAEQLRLESMVRANASRTGTAFTVS
                     IEAREGITTGISAHDRARTIATAIDPTKDHNDIVSPGHVFPLVAREGGVLVRAGHTEA
                     SVDLARLAGLYPAAVICEIMNEDGTMARMPDLVSFAQRHGLKIGTIEDLIAFRLRNDR
                     IVKPIARRQVESAFGGAFELYVYETTVEPVEHLALVKGDLTKPGPVLVRVHAVNMLSD
                     MLGIGLSADTADGSLIAQSMRAIAEEGRGVLVLIRDLRPKSVSSWIGERSGLTPRRVE
                     GNERRLVEIGVGSQILRDLGVRDMVLLTNSPDSVYVGLEGFGLKIVEQRRIG"
     gene            complement(2581..3174)
                     /locus_tag="VFX46_04125"
     CDS             complement(2581..3174)
                     /locus_tag="VFX46_04125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX5599804.1"
                     /translation="MFTGIITDIGEVIARDGGRFSIRSRYTADTIKIGASIACDGCCL
                     TVTAVEPQGTGSVFSVDTSNETRSKTTIDSWQAGRRINLERALTAGDELGGHIVTGHV
                     DGVARILDIQPDGESRRFTFEAPEHLALYIAPKGSVALDGTSLTVNEVAGNRFGVNVI
                     PHTLTVTTWGAKTAGQLVNLEVDIFARYVARLMEFRR"
     gene            complement(3228..>3432)
                     /locus_tag="VFX46_04130"
     CDS             complement(3228..>3432)
                     /locus_tag="VFX46_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599805.1"
                     /translation="GPAIWRSFARAGFVDEVIAFQARAEQTREANRPPPSAYLPGLDL
                     TLVARQEVGADVMMIYRRRIRQH"
BASE COUNT          623 a         1101 c         1071 g          637 t
ORIGIN      
        1 gaaatgcaag cgagaggagg taccccaccg gccgtgcggc ctttgcagcc aggtccgata
       61 cgttgacggc caaggccttc caggcaacgt cctcgggtgc atcttccggg cggaagtgaa
      121 tgccctcggc aatggcgtcg gtcttcagga ccagatcgtg gccagcctcc ggtgtcagtg
      181 ccgcgcagtc ctcctcgaga ccatatgcgc ccgggaaccc gaccgtcagc ggggcgaagt
      241 agctctgaat gaggctgtcc tcatccttga tctggtcatt attagtcatt cgacccgacc
      301 cggctctcac ttccacttgc gggaataagg gtacggtttt cggcttcaaa cgtctcgccg
      361 ggggagccac gccgtcgtgc tgcctctccg ccaagtgggg gctaactacc gcgccgacca
      421 ggcttggcgt cgagctccga cgggcgcagc cgccgcgcaa tcttgtcgag cacaccgttc
      481 acgacccgcg gctcgtcctc ggagaagaaa tcgtgggcga tatcgatgta ctcgttgatg
      541 acgacacgcg ccggcacgtc ctgccgctcc agaagctcgt aaacccccga tctcaaaatg
      601 gcacgcaaaa ttgaatccac ccgggccagt cgccagccgg cggcgagttg ctgatcgatg
      661 aggggatcaa cgtcccgctg acgacggaca acgcctctca gcaactcggc gaagaatgtc
      721 ggatcggcac ccgcaatggc atcgcccggt tcggcctgcg gaaaccgcag ggtttggaat
      781 tcctcgatga cggcattgag gtccgtttgg gccaggtcca tctggtacag gccctgtacg
      841 gctgcgacgc gggcagcgct gcgcgcgctt cgcctttccg gcttgttttc gggagtcatg
      901 cgccttggcc ctggaactgc cgggctatgt ccaataactg aaggcaggcg cgtgccgcag
      961 caccaccctt gccatcccgg ccaccctccg cacgtgacag ggcctgcgcc tcggtatcca
     1021 ccgtaaggat gccattgccg accggcacgt tgtgtcggat tgcgacctcc atcagccaat
     1081 ggttcgcatt gttgcaaacg atgtcgtaat gcgaggtctc gccacggatc acgcaaccca
     1141 gcgcgactgc gccactgtac caggcgcttg gagccgttgc gggaatgagg cccgcagcca
     1201 cggcctgcat gagagcctgt ggaatctcga gcgcgcccgg aacggtcacc cggtcgtagg
     1261 tcgctccggc agcctcaagc tccgcgacgg caccctccag tagagcatcg gaaatatggt
     1321 cgtagaagcg gccttcaata atgagaatgc gatagcggag ccgcgccggc atggcgctcc
     1381 cggccgttgc tgtcgcgcgt gaaccccgtc cagtcatgat gaattagccg atcctgcgct
     1441 gctcgacgat cttcagcccg aatccttcga ggccgacgta gaccgaatcc ggagaattgg
     1501 tcagcagcac catgtcgcgg acgcccaggt cgcgcaggat ttgcgagccg acgccaatct
     1561 cgacgagccg ccgctcattg ccctccaccc tgcggggcgt cagacccgag cgctcgccga
     1621 tccaggagga gacggacttc ggtctgaggt cgcgaatgag caccagcaca ccgcgccctt
     1681 cttccgcaat ggctcgcatg gactgagcga tgagagatcc gtcggccgta tcagcgctca
     1741 aaccgatgcc gagcatgtca gacagcatgt tgacggcgtg gacgcggacc agcaccggcc
     1801 ctggcttcgt caaatcacct ttgaccagcg ccaagtgctc taccggctcc acggtcgttt
     1861 cgtagacgta aagctcgaag gcgccaccga acgcgctctc tacctgccgg cgtgcgattg
     1921 gcttcacgat ccgatcgttg cggagccgga atgcgatgag gtcctcgatg gtcccaatct
     1981 tgaggccgtg gcgctgggcg aacgagacga ggtccggcat ccgtgccatg gtgccgtcct
     2041 cgttcatgat ttcgcagatc acagcggccg ggtagagccc cgccagccgt gcgaggtcca
     2101 cggaggcctc cgtgtgcccg gctcggacga gaacgccgcc ctcccgcgcg accaggggaa
     2161 acacgtggcc cggcgagacg atatcgttgt gatctttggt cggatcgatg gcggtggcga
     2221 tggttcgtgc gcggtcgtgg gccgaaatgc cggtggtaat gccctcgcgc gcttcgattg
     2281 acacagtgaa cgccgtgccg gtccgcgagg cattggcccg caccatcgac tcgagccgta
     2341 gctgctccgc gcgcgctttg gtcagtgcca ggcagatgag cccgcgcccg tgcttggcca
     2401 tgaagttgat ggcgtcaggc gtcgccatct gcgccgcaat gacgaggtcg ccctcatttt
     2461 cgcggttctc gtcatcgacg agaatgacca tgcgcccgtt gcggaggtcc tccaggagct
     2521 ccggcgtgga ggagaggagc cctcgtccct ccgagtagga aactgtcttg agggagctgt
     2581 tcacctgcgg aactccatga gacgggcgac gtatcgggcg aaaatatcga cttccagatt
     2641 aacgagttgg cccgccgttt tggcgcccca ggtcgtcacc gtcaaggtgt gggggattac
     2701 gttgacccca aagcgattgc ccgccacctc gttcaccgtg agagaggttc cgtcgagcgc
     2761 caccgagcct ttcggtgcaa tgtagagagc caaatgctcc ggcgcctcga acgtgaatct
     2821 tcggctctcg ccatcgggct ggatgtccag aatgcgcgcg acgccgtcga cgtgaccggt
     2881 gacgatatgt ccgccaagct cgtccccagc ggtcagcgcc cgctccaggt tgatgcgtcg
     2941 cccagcctgc cagctatcga tggtcgtctt cgagcgggtt tcgttggacg tatcgacgct
     3001 gaacacactg ccggtgccct gaggctcaac agcggtaacg gtcaagcagc acccatcgca
     3061 ggcgatcgag gcgccgatct tgatcgtatc cgcagtatac cttgagcgga tggagaagcg
     3121 cccgccatcg cgggcaatga cctcgccgat atccgtgatg atgcctgtga acatggtgct
     3181 cgcgtaagtt gcttcgggct taggcgtcga ggccgatcgt catctgttca atgctggcga
     3241 atgcggcgcc ggtaaatcat cataacatca gcgccgactt cctgcctcgc tacaagtgtg
     3301 agatcgaggc cgggcagata ggcgctgggc ggcggccggt tcgcttcccg tgtttgctcc
     3361 gcgcgggcct gaaaggcgat cacctcgtcg acgaaacccg ctcgcgcgaa ggagcgccat
     3421 atcgccggcc cg
//
LOCUS       DASUMG010000248         3301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_433463, whole genome shotgun sequence.
ACCESSION   DASUMG010000248 DASUMG010000000
VERSION     DASUMG010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433463"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VFX46_04135"
     CDS             complement(<1..670)
                     /locus_tag="VFX46_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599806.1"
                     /translation="MAFDALANRPLTVVSWGGAYQDAQRKVYFEPFKATGAQLIDESW
                     DGGVGVLRSKVQGDTDSGWDVVQVESEELELGCDEGMFLPIDWDKIGGKDRYLAAAQH
                     ECGVGTIMWNFVLAYDKDKLKEAPTGWADFFDTKKFPGKRALRQGPKSNLEIALMADG
                     VPPADVYKVLSTPEGVERAFKKLDTIKGDLIWWKSGAQAPQLLASGEVVMTSAYNGRI
                     DAAIR"
     gene            complement(821..1906)
                     /locus_tag="VFX46_04140"
     CDS             complement(821..1906)
                     /locus_tag="VFX46_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5599807.1"
                     /translation="MGASDSFVRFDNVDKTYDGAVLVVSGLNLDVHRGEFLTLLGPSG
                     SGKSTILMMLAGFEMPTRGLITLDGTDITRLPPHKRGLGMVFQNYALFPHMTVAENLA
                     FPLQVRGVPRSAIREKVARALEMVRLPNLGDRMPSQLSGGQQQRVALARALVFEPLLV
                     LMDEPLGALDKQLREHMQYEIKQLHDQLGVTVVYVTHDQSEALTMSDRIAVLNEGRIQ
                     QIGSPRSIYDRPDTAFVAQFIGENNRIEGTVRTLTNGIAAVEVDGGCTVLAEASDAVT
                     TGDRVLVSLRPERLLIGAAAADATNRFDGRILEVIYHGDHLRLRLEALGRDDFIVKTP
                     VTGTDGLTTGANVIIGWREADGRALPL"
     gene            2303..>3301
                     /locus_tag="VFX46_04145"
     CDS             2303..>3301
                     /locus_tag="VFX46_04145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX5599808.1"
                     /translation="MTATSQKGMSRQPGRWLGLALTIVAACTFASGPIWAQSGYATQA
                     KQAILIDANDGSVLFQHNADERMHPASMSKLMTLVMVFRALKSGQLKMDDEFVMSVNA
                     WRTGGAPSGTSAMFVPVNEKVTVSELLQGIIVQSGNDASICVAENIAGTEEAFAEQMT
                     RFGREIGLKSTTFKNSTGLYHPEHLTTARDLARLAQYVINEFPEYYPMFAQPDFRYRR
                     HRFVNRNPLLNMDLGVDGLKTGYIKEGGYGMVASAKQGERRLIAVVNGLPTAAKRREE
                     AARLLEWGFRNFTEFRLFDEGEIVGRARVWGGDQFYLPLTGAEGGVQVLLPRSAANQ"
BASE COUNT          648 a         1025 c          998 g          630 t
ORIGIN      
        1 tgcggatggc tgcatcaatg cgcccgttgt aggcggaggt catcaccacc tcgccggatg
       61 ctaggagctg cggggcctgc gctccagact tccaccagat gaggtcgccc ttgatggtgt
      121 cgagcttctt gaacgcccgc tcaacgccct ctggcgtgct caggacctta tagacgtcag
      181 ccggtggaac accgtcggcc atcagcgcta tttcgaggtt gctcttcggg ccttggcgca
      241 gggcccgctt gcctgggaat ttcttggtgt cgaagaagtc ggcccagccc gtgggcgctt
      301 ccttcagctt gtccttgtcg taagcgagaa cgaagttcca catgatcgtg ccgacgccgc
      361 actcatgctg cgcggccgcc aggtaacgat ccttgccgcc aatcttgtcc caatcgatcg
      421 gtaggaacat gccttcgtcg cagccgagct ccagctcctc gctctcgacc tgcacgacgt
      481 cccagccgga atccgtgtcg ccctggactt tggagcgcag cacgccgaca ccgccatccc
      541 agctctcgtc gatcaattga gcgcccgtgg ccttgaaggg ctcgaaatag actttccttt
      601 gtgcatcctg ataggcgccg ccccaggaga cgaccgtcag cggacggttg gccaatgcat
      661 cgaaggccat caaaccggcg ccggccacca ccgcggccaa gaccaattta ctcctcagtc
      721 ccatgcgcat cctccctttg catatcgata ccaaaccctt gcacggcccg ctattctggc
      781 gggacataca tctcgaagcc gaaacagtat tcctgcccat tcatagcggc aaggcgcgtc
      841 cgtcggcctc tcgccacccg ataatcacgt tggcccctgt ggtcaggcca tccgtaccgg
      901 tgaccggagt cttgacgatg aagtcgtcgc gtccgagcgc ctcgagacgt agacgaagat
      961 ggtcgccgtg atagatcacc tcgagaatgc gtccatcgaa ccggttggtg gcatccgctg
     1021 ccgccgcacc gatcagaagc cgttctgggc ggagtgagac aagaacgcgg tcgccggtgg
     1081 tgacggcgtc gctcgcctcg gccagcacag tgcacccgcc gtccacctcg acggctgcaa
     1141 tgccgttcgt cagcgtgcga actgtccctt cgatgcgatt gttctcgccg atgaattggg
     1201 cgacgaaagc ggtatcggga cggtcgtaga tcgagcgggg cgagccgatc tgctgaatcc
     1261 gcccctcgtt caaaacggcg atgcgatcgg acatggtcag cgcttcgctc tgatcgtgcg
     1321 tgacgtagac caccgtcacg ccaagctgat catgcagttg cttgatctcg tactgcatgt
     1381 gctcgcggag ctgcttgtcg agagccccaa gtggctcgtc catgagcacc aggagaggct
     1441 cgaatacgag cgccctcgca agcgcgacgc gctgctgctg tcctccggaa agttgagaag
     1501 gcatgcgatc gccaaggttg ggcaggcgaa ccatctccag cgcgcgtgca accttctcgc
     1561 gaatagcgga gcggggcact ccccgcacct gcaaaggaaa ggcaaggttc tccgccacgg
     1621 tcatgtgcgg aaagagggca tagttctgaa agaccatgcc gaggccacgc ttatgcggcg
     1681 gcaggcgtgt gatgtcggta ccgtcgagcg tgatcaagcc gcgggtcggc atctcgaaac
     1741 ccgcgagcat catcaggatc gtcgatttgc cggagccgga cgggccgagg agggtcagga
     1801 actcgccgcg gtggacgtca aggttcaacc cggacacgac aagcactgcg ccgtcgtacg
     1861 tcttgtcgac attatcgaac cggacgaagc tgtcggatgc ccccattgat cctcggtacc
     1921 aatatttcct atgacatcgc tacgaatcga gagctgatcc gaacggccca tcggttccag
     1981 gccgtgcccg gctcgtagag gtcgcgattc gggtaggtag cccgctcgag ccttgccgtc
     2041 gtttagcagg cgtcagcccg acctgcaatt tgatccgaac gcccgtttgc tgtcagcttg
     2101 cgcccgaaag gcgtgcagag tttctatcgc aacgtaaacg cggtcataaa gttaactccc
     2161 gcgagtatcg ccgtagcgac cggcaatcta ttatgggaaa cggaccaaga tcaccggtcg
     2221 acattgacgc gaacgccgcc ggctacagta taccccggat accctcgtgg tcgtcagatc
     2281 gagcgagcaa cactggagag ccatgactgc aacttcacag aaaggcatgt cacgccagcc
     2341 tggtcgttgg ctcggcttgg cgttgacgat cgttgcggcg tgcacgttcg ccagcggtcc
     2401 catatgggcg caaagtggct acgccacaca agccaagcag gcaatcctga tcgacgccaa
     2461 cgatggatct gtgctcttcc agcacaatgc ggacgagcgc atgcatcccg ccagcatgag
     2521 caagctcatg accctcgtta tggtgttcag agcgctcaag tccgggcagc tcaagatgga
     2581 cgatgagttc gtgatgagcg tgaacgcgtg gcggacgggc ggcgcgcctt cgggcacctc
     2641 ggcgatgttc gtcccggtca acgaaaaggt gactgtttcc gagctgctgc agggtatcat
     2701 cgtccagtcc ggcaacgatg catcgatctg cgtcgccgag aatatcgccg gaactgagga
     2761 ggctttcgcc gagcagatga cccgcttcgg gcgcgaaatc gggctcaagt cgacgacgtt
     2821 caagaactcc accgggctct atcaccccga gcatctgacg actgcgcgcg atctcgcgcg
     2881 actggcgcag tacgtcatca acgagttccc ggagtactat ccgatgttcg cgcagcccga
     2941 tttccgctac cggcggcaca gattcgtcaa ccgcaatccg ctgctgaaca tggacctcgg
     3001 ggtcgacggg ctgaagacgg gctacatcaa ggaaggcggc tacggaatgg tcgcctcggc
     3061 aaagcagggt gagcgccgtt tgattgcagt agtcaacggc cttccgaccg cggcgaagcg
     3121 ccgagaagag gcggcacgtc tccttgaatg ggggttccgc aacttcacgg aattccggtt
     3181 gttcgacgag ggcgagatcg tcggccgtgc acgcgtgtgg ggtggcgacc agttctatct
     3241 gccgctgacg ggtgccgagg gcggtgtcca ggttctcttg ccgcgttcgg cagcgaacca
     3301 a
//
LOCUS       DASUMG010000249         2580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_433611, whole genome shotgun sequence.
ACCESSION   DASUMG010000249 DASUMG010000000
VERSION     DASUMG010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433611"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VFX46_04150"
     CDS             complement(<1..770)
                     /locus_tag="VFX46_04150"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5599809.1"
                     /translation="MSKTTEAGRRKLRSQAWFDNPENIDMTALYLERMTNYGLTIDEL
                     QSGKPIIGIAQSGSDITPCNRHHLELAKRVREGIREAGGIAFEFPVHPIQETLKRPTA
                     ALDRNLQYLSLVEVLYGYPIDGVVLTTGCDKTTPAQIMAAATVDIPAIVLSGGPMLNG
                     WYNGERTGSGTIVWLAREMLARGEIDNKQFIEMVASSAPSAGHCNTMASAAEALGMSL
                     PGCAAIPAPHRDRAQMAYLTGKRAVEMVFEDLRPSKILT"
     gene            990..2222
                     /locus_tag="VFX46_04155"
     CDS             990..2222
                     /locus_tag="VFX46_04155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX5599810.1"
                     /translation="MHVVIIGAGIIGLAVAHKLLDQGHAVTLIDRGELSAASRGNAGI
                     IAHVDIQPLASPRMILQAARWLIDPVGPLSVRPAYLPRLAPWLARFLLASTPSRIERS
                     KQGIIPLQLMALPAWERLLSRLGLRRELNRNGMLYVFESIEAFEAMKPVYREQVELGI
                     QLELVDGTVLRTLEPALSDRFQRAAFFPGVAHVNDPFDLAVKLTSAASDRGAQLVEGS
                     VSGIAIASGDEPIVVLEGGRTISADRIVVAAGAWSKRLAACLGDKIPLDTERGYNTTI
                     PAPGVTLSRPVMFEEHAFVLSPLRTGLRVGGAVELASTSASPNWQRVEAMLAKAQAFV
                     PGLRDEGRVDWMGCRPSLPDSLPVISTARWSSKVVYAFGHAHHGLTQAAITAELVGAL
                     VAEQAPPIDLTPYNAQRF"
     gene            2238..>2580
                     /locus_tag="VFX46_04160"
     CDS             2238..>2580
                     /locus_tag="VFX46_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006088903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX5599811.1"
                     /translation="MATHTFFCIDGHTCGNPVRVVAGGAPALSGKTMLERRAHFLAEF
                     DWIRTALMFEPRGHDMMSGSILMPPTREDCDVGILFIETSGCLPMCGHGTIGTVTIAI
                     ERGLVQPRTPGQ"
BASE COUNT          427 a          816 c          842 g          495 t
ORIGIN      
        1 gtcaggatct tcgaaggccg caagtcctcg aacaccatct cgacggcccg cttgcccgtg
       61 agataggcca tctgcgcgcg gtcgcggtgg ggcgccggga tcgctgcgca gcccggcaag
      121 ctcatgccga gcgcctcggc ggccgacgcc atcgtgttgc agtgcccggc ggacggcgcc
      181 gaggaggcca ccatctcgat gaactgctta ttgtcgattt cgcccctggc cagcatctcg
      241 cgcgccagcc agacgatggt gccggagccg gtgcgctcgc cgttgtacca gccgttgagc
      301 atcggcccac cggaaaggac gatggccgga atatcgaccg tcgccgcggc catgatctgg
      361 gcgggagtcg tcttgtcgca accggtggtg agaacgacgc cgtcgatcgg gtagccgtag
      421 agcacctcga caaggctcag atactgcagg ttgcgatcaa gcgccgctgt cgggcgcttc
      481 agagtctcct gaatggggtg gacgggaaac tcgaaggcga tgccacccgc ctcacggatg
      541 ccttcgcgga cgcgctttgc gagctccagg tggtgccggt tgcaaggcgt gatgtccgaa
      601 ccgctctggg cgatgccaat aatcggcttg cccgattgca gctcatcgat agtgaggccg
      661 tagttcgtca tgcgctccag gtagagcgcc gtcatgtcga tgttttccgg gttatcgaac
      721 caggcctggg agcgcagctt tctgcggcct gcctctgtcg tcttggacat gttccatccc
      781 ttaagtcggc ttggggccaa tccagcgctc accgaaggac ccgcgctaaa ggcccacccg
      841 caccgatatc acgccgcctg cgtgcgcgcg tcaacaaaac acgctacagc caggccgttt
      901 ttcggggagc aactgtgcag acctcgacat cgccgccact gtcgggcatg aaagccggga
      961 agttggggga accgcgtcgg accgctcgta tgcacgttgt catcatcggg gcgggcatca
     1021 tcggattggc agtcgctcac aagctgctcg atcagggaca tgccgtgacc ctgatcgatc
     1081 gtggcgagtt gagcgccgcc tcacgcggca atgcggggat catcgcgcat gtcgacatcc
     1141 agccgctggc ctcaccccgg atgatcctgc aggcagcacg ctggctgatc gatcctgtcg
     1201 gaccgctttc ggtgcggcct gcctatctgc cccgattagc gccctggctc gcgcgttttc
     1261 tgctcgccag cacaccttcg cgcatagaac gttcgaagca gggcatcatt ccgctgcagc
     1321 tgatggcact cccggcgtgg gagcggctgc tttcgcggct tggcctgcgc cgcgagctca
     1381 atcgcaacgg catgctctac gtcttcgaga gcatcgaggc gttcgaggcg atgaagcccg
     1441 tgtaccggga gcaagtcgag cttggcattc agctcgagct cgtcgatggc acagtgcttc
     1501 ggacgctcga gcccgctttg tcggaccgct ttcagcgcgc ggcattcttc ccgggcgtcg
     1561 ctcacgtgaa cgatcccttc gatctggctg tgaagctgac ctctgcagcg agcgatcgtg
     1621 gagcgcagct cgtggagggg tccgttagcg gaatcgccat cgcaagtggc gacgagccca
     1681 tcgtcgttct ggaaggaggc cggacgatct ccgcggatcg gattgtggtt gccgcggggg
     1741 cgtggtcgaa gcggcttgcg gcgtgcctcg gtgacaagat tcctctcgac accgagcggg
     1801 gttacaatac aaccataccc gcgcccggcg tgacgctcag ccgcccggtc atgttcgagg
     1861 agcacgcttt cgtcctgtct ccgctgagaa cgggccttcg cgtcggtgga gccgtggagc
     1921 tggccagcac gtccgcatca cccaactggc agcgggtcga ggcgatgctc gccaaagcgc
     1981 aggcctttgt ccccggcctc agagatgagg gacgcgtcga ttggatgggc tgccggccat
     2041 cgctgcccga ctcgctgccc gtgatttcga cggcgcgctg gagcagcaaa gtcgtttacg
     2101 cgttcggtca cgcgcatcat ggtctgacgc aggcggccat tactgcggag ctggttggcg
     2161 ctcttgtcgc tgagcaagct ccgcctattg atcttacgcc gtacaacgct cagcgcttct
     2221 gatcaggagc acgcaacatg gcgacgcaca cgttcttctg catcgacggg catacgtgcg
     2281 ggaatccggt gcgggtggtc gccggcggtg ctccggcact cagcgggaag acgatgttgg
     2341 agcggcgcgc gcacttcctc gccgagttcg attggatccg gacggcgctg atgttcgagc
     2401 cgcgcggaca cgacatgatg tccggctcca tccttatgcc gccgacgcgg gaggactgtg
     2461 acgtcggtat tcttttcatc gagacatccg gctgcctgcc gatgtgtggg cacggcacta
     2521 tcggcacggt gaccatcgcg atcgaacgcg gactggtgca accgcggact cctggtcagc
//
LOCUS       DASUMG010000250         4137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_353865, whole genome shotgun sequence.
ACCESSION   DASUMG010000250 DASUMG010000000
VERSION     DASUMG010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353865"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFX46_04165"
     CDS             <1..896
                     /locus_tag="VFX46_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5599812.1"
                     /translation="CEETGVPYRRCGKLLVATAEDQLPKLKAIHGTASRNGVDDLVAL
                     SAAQAKELEPEVSCVAALLSPSTGIVDTHALMLAILGHAEADGAQLVLNSPVRSITAV
                     KPGLFSLAIGAPGDSETTVTCNKLVVSAGLHASRLAETMEYPSGYRPPPTYYAKGQYY
                     ALSGRSPFSRHIYPMPHGAWLGLHATVDLSGRCKFGPDIEWIPQIDYTFQPEKLAQFL
                     DFIRTYYPGLDPERLHPDYTGIRPKLYREGEPVPDFAIHAEAQHGHPGLVMLFGIESP
                     GLTAALAIGEYVARLIRGASN"
     gene            complement(893..1348)
                     /locus_tag="VFX46_04170"
     CDS             complement(893..1348)
                     /locus_tag="VFX46_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018066709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599813.1"
                     /translation="MDYLVLLTGLACALALVGLGGAFYEFTVVDPFWPKRPDLIQPQR
                     GGISRKQFWIPAHISFEIVLIVTLIVAWSAPDVRFWLIVAVISHATMRIWSAFDFIPK
                     ALAFETADPQSVTETEARNWTRRSIMRLPLALLTSFATLAAFFETARLS"
     gene            complement(1389..2354)
                     /locus_tag="VFX46_04175"
     CDS             complement(1389..2354)
                     /locus_tag="VFX46_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX5599814.1"
                     /translation="MLLTLRQLEILVAAADAPTFSEAAARIGISQPSLSETIRRAELE
                     LNVKLFDRTTRSVTLTEEGRYVVAVAREMIRNFRQGLENIARWSGTRRGRITVAGLPS
                     VACAVLPAAIRRFVQEHPDVEINVHDLPHERAIAAVEHGMADLALTIRPAKLKECRFE
                     ELGADRLYLVCPPDHPLMKKEKVSWRDLAPYPFVGLSRGSSVRRMTDAAFVNREIFIE
                     PAYELEQIPSAVALVEAGLGISALPALTLTMVRGARVATKPLFDPVMKRRIGVVTLAG
                     RQPSNFILSFIKVLRETFNELRLNSGRDSELRFPHPMRVPFPRQS"
     gene            2445..3818
                     /locus_tag="VFX46_04180"
     CDS             2445..3818
                     /locus_tag="VFX46_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX5599815.1"
                     /translation="MVSRVFIGCGAGFAGDRTDAGIPVVRALAECDGPRYIIYETLAE
                     RTLALAQVARRNSSGPGYNPALDKFLRPILRDCLAAGIKIVGNFGAANPRGAAHRIAE
                     LARELGCGRLKIAFVEGDDITSRLSPAEFAQREVGGQLLAGHPEIVAANVYLGAEPIA
                     EALAKGADVVVTGRVADPSLVLGPLLHSFGWPLDDLDRIAAGTLAGHLLECGAQLTGG
                     YFADPGYKDVPGMDNMGYPVAEIDADGCVVVGKPAGTGGLVDRRTVTEQILYEIHDPA
                     AYLTPDVVLDITEVEIEEIGENRVRVTGARGHPKPEKLKATVCIDGGFLAEAEISYAG
                     PNAAARARLAIDTVRKRVARQLPHLTLRTDAIGVMSVHADLAGEAAQEAWLRAPDDSR
                     VRFAAQFPDTETAEILLGEVEALYCAGPAGGAGLRRHITPRLLSASCLIERDLVTPTV
                     SMLEGAP"
     gene            3815..>4137
                     /locus_tag="VFX46_04185"
     CDS             3815..>4137
                     /locus_tag="VFX46_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599816.1"
                     /translation="MSDFVEVPLHRIAYARSGDKGNRLNIALIAAAPRYYQSIAEQMT
                     ESRVAAIFATRRPTRVVRYDLPKLCAFNFVLDDVLEGGVNSSLGLDGHGKSLSYLLLA
                     APIRVS"
BASE COUNT          816 a         1260 c         1260 g          801 t
ORIGIN      
        1 tttgcgagga gactggcgtg ccctaccgcc gttgcgggaa gcttctcgtt gcgacagcgg
       61 aggaccagct gccgaagctg aaggccatcc acggaacggc gtcgcgcaac ggcgtcgacg
      121 acctcgtggc gctttcagca gcgcaggcaa aggagctgga gccggaggtg agctgcgtgg
      181 cggcgttgct ctcgccttca accggcatcg tcgacacgca tgccctgatg ctggccattc
      241 tcggtcatgc ggaagcggac ggcgcgcagc ttgtgctgaa cagcccggtt cggagcatca
      301 cggcggtcaa gcctggactg ttctctctcg ccatcggagc gcccggcgac tctgaaacaa
      361 cggtgacgtg caacaaactt gtcgtttctg ccgggctgca cgcctcacgt cttgctgaga
      421 cgatggagta cccttctggc taccggccgc caccgaccta ctatgcaaag ggacaatact
      481 acgccctgtc cggccgctcg cccttcagcc gtcacattta tcccatgccg cacggcgcgt
      541 ggctgggcct ccacgcaacc gtggacttgt ccggccgctg caaattcggc cccgatatcg
      601 agtggattcc gcagatcgac tacacatttc agcccgagaa gctggcgcag tttctcgatt
      661 tcattcggac ctactatcca gggctcgatc cagaaaggct gcatcctgac tacacaggaa
      721 ttcgcccgaa actctatcgc gagggcgagc ctgtgccgga cttcgccatc cacgccgagg
      781 cgcagcacgg ccaccccggg cttgtcatgc tttttggcat cgaaagccca ggtctgaccg
      841 ccgcgctcgc cattggcgag tacgtcgctc gactcattcg aggggcatcg aattaggaca
      901 atcgagcggt ctcgaaaaag gctgccagcg tcgcgaaaga ggtcagcaaa gccaatggca
      961 accgcatgat gctgcggcgg gtccaattcc gagcctcagt ttcagtgacg ctctgcggat
     1021 cggcggtctc gaaagcaagc gccttcggga tgaaatcgaa tgccgaccag attcgcatgg
     1081 ttgcgtggct tatcacggcg acgatcagcc aaaacctgac gtctggagcc gaccacgcga
     1141 cgatcagcgt tacgatcagc acgatttcga acgaaatatg cgcgggaatc cagaactgct
     1201 ttctcgaaat tccgccgcgc tgcggttgga tgagatcggg gcgctttggc cagaatgggt
     1261 ccacgacggt gaactcatag aatgcacctc cgagccccac cagcgccaat gcacacgcca
     1321 atccggtcag aagcacaagg tagtccattc ctcttcctcg tcgatgaagt ggcgtgtccc
     1381 ttttggggtc aactttggcg aggaaaaggc accctcatgg ggtgaggaaa acgaagttcc
     1441 gaatctctgc ctgaattgag ccggagctca ttgaaggttt cccgcagcac cttgataaag
     1501 ctcaagatga aatttgacgg ctgacgacct gcgagcgtga cgacgccgat tcgccttttc
     1561 attacgggat cgaaaagcgg ctttgtcgcg acccgcgcac ctctgaccat tgtgagcgtc
     1621 aaagcaggaa gcgcggaaat accgagccca gcttcgacca gcgcaacggc ggacgggatt
     1681 tgctcaagct cataggcggg ctcgatgaaa atctcgcggt tgacgaatgc tgcatcggtc
     1741 attctgcgga cggatgatcc gcgagagagc ccaacaaacg gataaggcgc gagatcacgc
     1801 caactcactt tttccttttt catgagcgga tgatccggcg ggcacaccag atagagtctg
     1861 tcggcaccga gctcttcgaa acggcactcc tttagcttcg ccggccgaat ggtcagtgca
     1921 agatcggcca tgccgtgctc aacagccgcg atggcccgct cgtgcggcag atcatggaca
     1981 ttgatttcaa cgtcggggtg ctcctgaacg aagcgccgga tggcagcggg aagaaccgcg
     2041 caagcgacag aaggcaggcc agcgacggta atccggccgc ggcgcgtacc gctccaccgg
     2101 gcaatattct ccaggccctg gcggaagttg cggatcatct cccgggcgac tgcaacgacg
     2161 tagcgcccct cttctgtcag cgtgaccgag cgcgtggtcc gatcgaacaa cttcacattg
     2221 agctcgagct ctgcccgacg gatggtctcg ctgagtgatg gctgagatat cccgatcctg
     2281 gcagccgctt cactgaaggt cggcgcatct gcggctgcga ccaggatctc cagctgccgc
     2341 aaagtgagaa gcataggctc gtcctatatt ttcacaggtc gccgttattt aacacgatga
     2401 atagcctttg ctagatttgt tctggggagg agaatctagg accgatggtg agccgcgttt
     2461 tcatcggttg tggagctggt ttcgccggcg accggacgga cgctggcatt ccggtcgtcc
     2521 gcgcgctcgc agaatgcgac ggcccgcggt acatcattta cgagacgctt gccgagcgga
     2581 cgctggcgct ggcgcaggtg gcgcgccgca actcctcagg gccaggctac aacccggcgc
     2641 tcgacaaatt cctccgtccg atcctgcgag actgccttgc tgccgggatc aagatcgtcg
     2701 gtaacttcgg agcggccaat ccgagaggtg cagcccaccg catcgcggag ctggcgcgcg
     2761 agctcggttg cggccggttg aaaatcgcct ttgttgaagg cgacgatatc acaagccgcc
     2821 tgtcacccgc cgagtttgct cagcgcgagg tcggaggcca gctccttgct ggccatcccg
     2881 agatcgtggc ggcgaacgtg tatttgggcg ccgagccgat cgccgaggcg cttgcaaagg
     2941 gggccgacgt tgtcgtgacc ggcagggtcg ccgatccttc cctcgtcctc ggacctttgc
     3001 ttcacagctt cgggtggccc ctggacgacc ttgatcgcat cgcggcagga acgctcgccg
     3061 gccatctcct ggagtgtggc gctcagttga ccggcggcta cttcgcagat cctggctaca
     3121 aagatgtacc gggtatggac aatatgggct acccggtcgc tgaaatcgac gccgacggat
     3181 gcgtggtggt cgggaaaccg gccggaactg ggggactagt cgaccgtcgg acagtgacgg
     3241 agcagattct ctacgagatc cacgatccgg ccgcctacct tacccctgac gtggtgctcg
     3301 acatcactga ggttgagatt gaagagattg gcgaaaatcg cgtgcgcgtg accggcgcgc
     3361 gcggacaccc caagccggaa aagctgaagg ccacagtttg tatcgatggc ggttttcttg
     3421 ctgaggctga gatttcctat gctggcccca acgcggcagc acgcgcgagg ctggccatcg
     3481 atacggttag gaaacgcgtc gcccggcaat tgcctcatct gacacttcgg acagatgcga
     3541 tcggtgtgat gagcgttcat gcggacctgg ccggcgaagc cgcgcaagag gcttggctgc
     3601 gtgcaccgga tgacagtcgc gttcgcttcg ccgcgcagtt tcctgacacc gagacggccg
     3661 aaattttact cggcgaggtc gaggccctct attgcgccgg tccagctgga ggagcgggtc
     3721 tccgcaggca cataacgcca aggctgctca gcgcctcgtg tctgatagag cgcgatctgg
     3781 tgacccccac ggtatcaatg ctggagggcg cgccatgagc gatttcgtcg aggttccgct
     3841 ccacagaatc gcctacgccc gctcgggcga caaaggcaac cggctgaata tcgcgttgat
     3901 cgcggctgct ccacgctact atcagtcgat cgcggaacag atgaccgaga gccgcgtagc
     3961 ggccattttc gccacccgcc ggccgacgcg cgtcgtccgc tacgacctgc cgaaactttg
     4021 cgccttcaat tttgtgctcg atgacgtgct cgagggcggt gtgaactcca gtcttggcct
     4081 tgatggccac ggcaagagtc tgtcgtacct gcttctcgcc gcacccatcc gcgtttc
//
LOCUS       DASUMG010000251         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_594533, whole genome shotgun sequence.
ACCESSION   DASUMG010000251 DASUMG010000000
VERSION     DASUMG010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594533"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..1011
                     /locus_tag="VFX46_04190"
     CDS             448..1011
                     /locus_tag="VFX46_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HEX5599817.1"
                     /translation="MRIIRQMRALSVALIGALTMLGSVPQAKAGPAAAEIDLAVQDTL
                     EEFFYRVPGSRELVAKSAAVLVFPTVYKAGFGIGGEYGEGALLSRRRTIDYYNLISAS
                     IGFQLGAQARSIVIVFMTPEALASFRRVDGWKVGVDGSIALINIGIGGSIDTTRITNP
                     IVGFIFDNKGLMYNLTLEGSKISRIYR"
     gene            1126..>2047
                     /gene="aroC"
                     /locus_tag="VFX46_04195"
     CDS             1126..>2047
                     /gene="aroC"
                     /locus_tag="VFX46_04195"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX5599818.1"
                     /translation="MSYNTFGHLFRVTTWGESHGPAIGCVVDGCPPGVPLTAEDIQAY
                     LDRRRPGQSRYTTQRREPDQVEILSGVFVDDNGRQVTTGTPISLVIRNVDHRSKDYGD
                     IKDAFRPGHADYTYYEKYGIRDYRGGGRSSARETAMRVAAGAIARKILSGVTIRGALV
                     RIGPHEIDRTRWDWAEVDSNPFFAPDHGIVQTWASYLDQIRKAGSSAGAVVEIVAEGV
                     PPGLGAPVYGKLDQDLASALMSINAVKGVEIGAGMAAAQLRGEENADEIRIGPDGRPQ
                     FLTNNAGGILGGISTGQPIVCRFAVKPTSSI"
BASE COUNT          395 a          611 c          631 g          410 t
ORIGIN      
        1 ttcaagttaa ctcttagtgc aaatttgatg tgggccggtc cgccgccacc gcggtgcgtt
       61 cgcgagtcgg atcgaggcgc tcgccgccac atatctgccg aaacgttgac caatggttct
      121 caattggctg gcaatcgctc gccagataca cacctcgtcg ttactcggcg tgccgctcct
      181 gagaaagaaa ggggaattga agagcctggc ggggtggagc tagtcgagaa agtaaaagag
      241 gcagccgctc gcgcgacgag ctttcgccat gaaactacaa cgacggcact ttgttgcgcg
      301 acctgatgac cccgtgccgc tagcgtggcc ctcacacaac gctcccacga aaacatccaa
      361 ccagcctgag tctggccacg gcggaatgct acgcttcaga tcgtggtgag tcggaggttc
      421 cgaccgccgt cgaaacgagg gaacaccatg cgcatcattc ggcagatgag agcattgtcg
      481 gtggcgttga tcggtgcgtt gacgatgctt gggtccgttc cgcaggctaa ggcaggaccg
      541 gcggcagccg agatcgatct tgcggtccag gataccttgg aggagttctt ctaccgagtg
      601 cctggctcgc gggagctggt tgccaagtcc gccgccgtgc tcgtgttccc caccgtctac
      661 aaggccggct tcggtatcgg cggtgagtat ggcgagggag ccctcctctc ccgtcgcagg
      721 acgattgatt attacaatct gatctcggca tctatcggct tccagctcgg cgcacaggcg
      781 agatcgatcg tcatcgtttt catgacgccc gaagcgctgg cgagcttccg ccgggtggac
      841 ggttggaagg tgggtgtcga tggctccatt gccctgatca acatcggcat aggtggttcg
      901 atcgacacga ctcggatcac gaaccccatc gtcggcttca tcttcgacaa caagggcctg
      961 atgtataacc tcacgcttga gggctcgaaa atcagccgca tctatcgctg agcggcgctg
     1021 aaagtcgttc tgacctgtga tgctctgcgc atcgggcgtt gccgcctgat gctgtgtcgg
     1081 ttaagcctgc cgagcagatc gacattgccc ggctggttta tccgcatgtc ctacaatacc
     1141 ttcggccatc tatttcgtgt cacgacttgg ggcgagagcc acgggccggc gatcggctgt
     1201 gtcgttgacg gctgcccgcc gggcgttccg ctgactgcag aggacatcca ggcgtacctt
     1261 gaccgccgcc gtcccggaca gtcgcgctac actacgcagc gccgcgagcc ggaccaggtt
     1321 gaaatccttt ctggcgtgtt cgtcgatgat aacgggcggc aagtgactac cggcacgccg
     1381 atctcgctcg tgatccgaaa cgtcgatcac cgctccaagg actacgggga catcaaagat
     1441 gcgtttcgcc ccgggcacgc ggattatacc tactatgaga agtacggcat tcgcgattat
     1501 cgcgggggag gtcgatcctc agcccgcgaa acggccatgc gcgtggcagc tggggcaatc
     1561 gcgcgcaaga tcctgtcggg cgtgaccata cgcggcgccc tcgttcgtat cgggccgcat
     1621 gagatcgatc gaacacgctg ggactgggcc gaggtcgaca gtaacccctt cttcgcgccg
     1681 gaccacggga tcgttcagac ctgggcctcc tatcttgacc agatccgcaa ggctggctcg
     1741 tccgccggag cagtcgtcga gatcgtcgcc gagggagtgc cgccgggctt gggagcgccg
     1801 gtatatggaa agctggatca agaccttgcc tcggcgctga tgagcatcaa tgcagtcaaa
     1861 ggtgtcgaga tcggtgccgg catggccgca gctcagctca gaggagaaga gaacgcggac
     1921 gaaatccgta ttggccccga cggccgacct cagttcctga cgaataatgc aggcggcatc
     1981 ctcggcggga tttccacggg tcagccgatt gtttgccgct tcgccgtgaa gcccacatcg
     2041 tccatac
//
LOCUS       DASUMG010000252         2677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_515012, whole genome shotgun sequence.
ACCESSION   DASUMG010000252 DASUMG010000000
VERSION     DASUMG010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515012"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFX46_04200"
     CDS             <1..413
                     /locus_tag="VFX46_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5599819.1"
                     /translation="ERLQTTRDPRVLAMGDCAAAPWLGRNKLVPPRAQAAEQQARYLA
                     RAIPRLLAGQEVKPFRYNDLGSFVSLGEENTIGTLMGYLRGHRLRLEGFIAHLIYRWL
                     YKRHQAALFGWSAAILTTIGNWLRGTSRPRVKLH"
     gene            complement(449..769)
                     /locus_tag="VFX46_04205"
     CDS             complement(449..769)
                     /locus_tag="VFX46_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX5599820.1"
                     /translation="MRLVTTETIEEPVRHGELIYACAVSGANVLRDMREAITNTIGGE
                     MTRYEALLDQTIARALAKLAERAREKGYDGVLAVRISHPVITNGAVEVVVAGTGYRRV
                     PAVN"
     gene            complement(780..2582)
                     /locus_tag="VFX46_04210"
     CDS             complement(780..2582)
                     /locus_tag="VFX46_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599821.1"
                     /translation="MPAAFLHRTIAALTAFAVATAPLSEQASAAVKLTRADYEACQTG
                     DEQAFRAAVELITSKALQAGLAAIDFKAVVAEEWRRTGLDETLDKQVDLAVAEVHDES
                     SWGDLLQSLAYKEKSQELATAVAERVYRSEPVKVAIEELAVGVGKNVGRNIELATLDA
                     AEPALQCLQAFLGPRFGTTVSRVVANDAGKEFAIDPETARANVSTTSVLIEGSEGIAG
                     AVILLVRRQLANMARRIGHRIVGAVLGRLVSIVAGGVGVVLIAKDIWELRSGVLPIIA
                     EEMKSDATKQRVREELADSIARQLEEQVRDISVSTADRVVEIWREFRRAHAKVLELAE
                     QNPPFRAFLDAASPEQLPRIDELVGIIVADEGDTGVLKRLENGTLYDAVNRLTEPGMT
                     IARDLRSVEAALRWSTLAGDRLDRVVEWELYRRARADSFTPVSISRLLALDDRLAALR
                     LTSVERDARDVLFELDNDELRKLARSLAEPELQAMARYMTGLRPDASQRIMRAVAQNP
                     SKMQVLSSTRVRDAVLASRDQDAAVAMMLRNSSLPNPAVISADFQLVLDGQVSPILLW
                     ERHSIVISVLAFLALVVLLMFKRLLFGRRRRVTA"
BASE COUNT          484 a          892 c          806 g          495 t
ORIGIN      
        1 ctgaaaggct gcagacgacc cgtgatccgc gtgtgctcgc aatgggcgac tgcgctgcag
       61 ctccgtggct gggccgaaat aaactggtgc cgccgcgcgc gcaggctgcc gagcagcagg
      121 cccgctatct cgctcgggcc attccacgat tgctcgctgg tcaggaggtg aagccgttcc
      181 gctacaatga cttaggctct ttcgtatcac taggcgagga gaacacgatc ggcacgctga
      241 tgggctatct cagagggcat cgtctgcgcc tcgagggctt cattgcccac ctgatctacc
      301 gctggctcta caaacgccat caggcggcac tgtttggctg gagcgccgct attcttacga
      361 ccatcggtaa ttggctacga ggcacctctc ggccgcgggt gaagttgcat taacaatgcc
      421 ctttgaatct cgctgcgcga gggcgctgtc agttcaccgc gggtactcgc ctatatcccg
      481 tccctgcgac caccacctcg acggcgccat tcgtgatcac aggatgcgag atgcgcacgg
      541 caagcacgcc gtcataacct ttctcccgcg cccgttcggc aagcttggcg agggccctgg
      601 caatcgtctg gtcgagcaag gcctcgtagc gggtcatctc gccgccgatg gtattggtga
      661 ttgcctcgcg catgtcccgc agcacgttcg cgccgctgac cgcgcaggca tagatcagct
      721 caccgtgacg cacgggctcc tcgatcgttt cggtcgtgac gagccgcatg tctcccccgt
      781 taggctgtca ccctccgccg gcggccgaag aggagccgct tgaacatcag cagcacgacg
      841 agggcgagaa atgccaatac gcttatgacg atcgaatgcc tttcccacag caggatcggg
      901 ctgacctgcc cgtcaagaac aagttgaaaa tcggcgctga tcaccgccgg attggggagc
      961 gagctgttgc gcagcatcat ggcgacggcg gcatcctggt cgcggctcgc cagcacggcg
     1021 tcgcggaccc gagtcgacga gagcacctgc atcttggacg gattttgagc caccgcacgc
     1081 attatgcgct gactcgcgtc cggtcgaagg ccggtcatat accgtgccat cgcttgcagc
     1141 tccggctcgg ctaagctgcg ggcgagcttt ctcagttcat cgttgtccag ctcgaacagc
     1201 acgtcccggg catcgcgctc cacgctggtc aatcgcagcg ccgcgagcct gtcatcaagc
     1261 gcgagcaggc gagagatcga aaccggggtg aagctatcgg ccctggcgcg ccgatacagc
     1321 tcccactcga cgacgcggtc gaggcggtca ccggcgagcg tcgaccaacg tagcgccgcc
     1381 tcaactgacc ggagatcgcg cgcgatcgtc atgcccggct cggttagccg gttgaccgcg
     1441 tcgtagagtg ttccgttctc caaccgcttg agaacaccgg tatcgccctc gtcggcgaca
     1501 atgatgccca ccaactcgtc gatgcgcgga agctgctcgg gactcgcggc atccaggaac
     1561 gctctgaacg gcggattctg ctccgcaagt tcgagaacct tggcgtgggc gcggcggaat
     1621 tcccgccaga tctcgacaac gcgatcggcc gtcgaaacgg agatgtcacg cacctgctcc
     1681 tcgagctgcc gggcaatcga atccgcgagt tcctccctta cacgctgctt cgtcgcgtcc
     1741 gacttcatct cctcggcgat gatgggcagc acgccgctgc gaagctccca aatgtccttt
     1801 gcgatcagca cgacgccaac gccgccggcc acgatcgaca ccagccgccc gagcaccgcg
     1861 ccgacgatgc gatgaccgat gcgccgcgcc atgttggcga gctgacggcg gacgagcagg
     1921 atgaccgcgc ccgcgatgcc ctcgctgcct tcgatcagca cagaggtcgt cgaaacattt
     1981 gctcgcgctg tttccggatc gatggcgaac tccttgccgg cgtcgttcgc caccacccga
     2041 gagacggtgg tcccgaaacg cgggccgagg aacgcctgca ggcattgcag tgccggctcc
     2101 gccgcgtcga gggtcgccag ctcgatattg cggcccacgt tcttgcctac gccgacagcg
     2161 agctcctcga tggccacctt taccggctcg gaccggtaga cgcgctcagc caccgcggtc
     2221 gccagctcct gcgatttctc cttgtaggcg agagattgca ggagatcgcc ccagctcgac
     2281 tcgtcgtgga cctcggcaac tgcgaggtcg acttgcttat ccagagtttc gtcgagacct
     2341 gtacggcgcc attcctcggc cacgactgcc ttgaaatcga tcgctgcgag accggcttgc
     2401 agggccttgg atgtgataag ctcgaccgcc gcacggaacg cctgctcgtc gcccgtctga
     2461 cacgcctcgt agtccgcacg cgtgagcttc accgcggcgc tggcctgctc agacaggggc
     2521 gctgtcgcga ccgcgaaagc cgtcaatgct gcaatagtcc ggtgcagaaa agcagcaggc
     2581 acgcgcactc ccctgtgtct ctcgtcctcc aggcagagga gcgattatgg ttactccagt
     2641 atttatagct gatgaatttg gaaaccggga cacacgt
//
LOCUS       DASUMG010000253         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_194216, whole genome shotgun sequence.
ACCESSION   DASUMG010000253 DASUMG010000000
VERSION     DASUMG010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194216"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /gene="lon"
                     /locus_tag="VFX46_04215"
     CDS             <1..1573
                     /gene="lon"
                     /locus_tag="VFX46_04215"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX5599822.1"
                     /translation="RMPEAAAEYSMVRTYLDWLVELPWKLPEEPPIDIEQARKILDED
                     HYGLEKVKRRIIEYLAVRKLAPHGKAPILCFVGPPGVGKTSLGQSIARAMGRKFQRVS
                     LGGVHDEAEIRGHRRTYVGALPGNIIQAIRKAGSRNCVLMLDEIDKLGAGIHGDPAAA
                     LLEVLDPEQNSTFRDNYLGVPFDLSRVVFITTANMLDTIPGPLRDRMEVISLAGYTAD
                     EKLQIARRYLVRRQLEANGLKPEQVEITDEALKEIIQRYTREAGVRNLEREIGKALRY
                     AAVRIAEGDTHPIRIDASDLQGILGPPQFESEVAMRTSVPGVATGLAWTPVGGDILFI
                     EATRVPGSGKLILTGQLGEVMRESAMAALSIVKNRAEALNIDPAIFEKNDIHVHVPAG
                     AVPKDGPSAGIAIYVALISLLTRRCVRNDTAMSGEISLRGLVLPVGGIKEKVVAAARA
                     GIKRVILPARNRKDYEDIPEEARRDVEIIWIDHVEEAVAAAICSEEQKTAKTPREPEA
                     AEEAAAQPRAGEAAE"
     gene            complement(1579..>2141)
                     /locus_tag="VFX46_04220"
     CDS             complement(1579..>2141)
                     /locus_tag="VFX46_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX5599823.1"
                     /translation="APTVWMLGSSDYGAQVAAHFGLPYSFAHFITDGVGTEQALALYR
                     QLYRPSRRHPEPLATVCIWALAAETEEEAQWLFTSRAKSRLARDRGVYGPLDHPDAPL
                     PFELTPQEEARLAEMRRTAFVGTPKKVGERIRALANQLGIDEVAIVTWSHDPAARRKS
                     YELLAAEFGLQASQSAAGVGDKRAKR"
BASE COUNT          424 a          661 c          705 g          351 t
ORIGIN      
        1 acgcatgccg gaagccgcgg ccgaatacag catggtccgc acctacctcg actggctggt
       61 cgagctgccc tggaagctgc ccgaggagcc gccaatcgac atcgagcagg cgcgcaagat
      121 cctcgacgag gaccactacg gactcgagaa ggtcaagcgg cgcatcatcg agtatctcgc
      181 cgtccgcaag ttggctccgc acggcaaggc gccgatcctc tgcttcgtcg gaccgccggg
      241 tgtcgggaaa acctctctcg gtcagtcgat cgcccgcgcc atgggccgca agttccagcg
      301 ggtcagcctc ggtggcgtgc atgacgaggc cgaaatccgc ggccaccggc gcacctacgt
      361 cggcgcattg ccgggcaaca tcattcaagc catccgcaag gcgggttcac gcaactgcgt
      421 gctgatgctc gacgagatcg acaagctagg cgcgggcatc cacggcgacc cagctgccgc
      481 cttgctcgaa gtgcttgatc ccgagcagaa cagcacgttc cgcgataatt atctcggcgt
      541 gccgttcgat ctcagccggg tggtgttcat caccaccgcc aacatgctcg atacgatccc
      601 cggcccgttg cgtgaccgta tggaggtcat tagccttgcg ggctacacgg ccgatgaaaa
      661 gttgcagatc gcacggcggt atctcgtccg gcggcagctc gaggccaacg gcctgaaacc
      721 agagcaggtc gagatcacgg acgaggctct gaaagagatc atccagcgct acacacggga
      781 agctggtgtc cgcaatctgg agcgcgagat cggaaaggcg ttgagatacg ccgcagtacg
      841 catcgcggaa ggcgacacgc accccatccg cattgatgcc agcgatcttc aaggaatcct
      901 tgggccgccg cagttcgaga gcgaggtggc catgcgcacc agcgtgccgg gtgtggcaac
      961 cggtctcgcc tggacaccgg tgggcggcga catcctcttc atcgaggcga cccgcgtgcc
     1021 gggcagtggc aagctgatct tgacgggtca gctcggcgag gtcatgcggg aaagcgccat
     1081 ggcggcgctc agcatcgtca agaaccgcgc ggaagcgctc aacatcgacc cggccatttt
     1141 cgaaaagaac gacattcacg tccacgtgcc agcgggcgcc gtgcccaagg atgggccgag
     1201 cgcaggcatt gccatctatg tcgcgctcat ctcactgctg acccggcgtt gcgtgcgaaa
     1261 tgacacggcc atgtcgggag agatcagcct ccgggggctc gtgttgccgg tcggcggcat
     1321 caaggaaaag gtggtggccg ctgccagagc tgggatcaag cgcgttattc tgcctgcccg
     1381 caaccggaag gattacgagg acatcccaga ggaggcgcga agggacgtgg agatcatttg
     1441 gatcgaccac gtcgaggagg cagtcgctgc tgctatctgt tccgaggagc agaaaactgc
     1501 caagacgccg cgtgaacccg aggcagctga ggaagcagcc gcacagcctc gcgcaggaga
     1561 ggcagcggag tgatttggtc agcgttttgc gcgtttgtcg ccgacgcccg cggctgattg
     1621 cgatgcttgg agcccgaact ccgcggcgag aagctcgtag gacttgcggc gtgctgccgg
     1681 gtcgtgtgac cacgtcacga tggcaacctc gtcgatcccg agctggttcg ccagggcacg
     1741 gatccgttcg ccaaccttct taggcgtgcc gacaaacgcg gttcgccgca tttcggcgag
     1801 gcgggcttcc tcttgtggcg tgagctcaaa cggcaacggc gcatcagggt gatcgagcgg
     1861 tccgtacaca cctcgatcgc gcgccaatcg cgattttgcg cgcgaagtga aaagccattg
     1921 ggcctcctcc tcggtctcgg cagcgagcgc ccaaatgcag acagtcgcca gaggctcggg
     1981 gtggcgccgg ctcggccggt agagctggcg gtaaagggct aacgcctgtt cggtgccgac
     2041 accgtcggta atgaagtggg cgaaggagta gggaagccca aagtgagcgg caacctgtgc
     2101 gccatagtcc gagctgccga gcatccacac agtgggcgcc g
//
LOCUS       DASUMG010000254         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_917080, whole genome shotgun sequence.
ACCESSION   DASUMG010000254 DASUMG010000000
VERSION     DASUMG010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917080"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFX46_04225"
     CDS             complement(<1..555)
                     /locus_tag="VFX46_04225"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX5599824.1"
                     /translation="MTRELIYFADPMCSWCYGFAPVISAIEQHFRGRLPLRLVMGGLR
                     AGQTRPMRDQDKEYIREAWTRVSAASGQPFDFSFFEREGFVYDTEPACRAVVTMRLRQ
                     PSRALAFMERIQTAFYAENRDVTSREVLADLAAEEGEDRETFAAALLSPEARNETFRD
                     FLTAQEFGIEGFPTLIVGSGNQYAL"
     gene            804..2465
                     /gene="ettA"
                     /locus_tag="VFX46_04230"
     CDS             804..2465
                     /gene="ettA"
                     /locus_tag="VFX46_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX5599825.1"
                     /translation="MATPQYVFHMHRLSKTYPGGKQVLKDISLSFLPGAKIGVVGVNG
                     AGKSTLLKIMAGVIDDYQGEAWAAEGVRVGYLPQEPELDPTKDVLGNAMEGVAEKKAL
                     LDRYTELAANYSEETAEEMARLQDEIDAQNLWDLDQQVEQALDALRCPPGDADVTKLS
                     GGEKRRVALCRLLLSKPDILLLDEPTNHLDAETVAWLERYLKEYPGCVILVTHDRYFL
                     DNITEWTLELDRGQGIPYKGNYSSWLEQKAKRLEIEKNQEEAKQRTLKRELEWIRSSP
                     KARQAKAKARIQAYEKLAEEAGREQISHAQIQIPPGPRLGSVVLEVENLTKGYGDRLL
                     IENLSFKLPPGGIVGVIGPNGAGKTTLFRMITGQEKPDAGTIRLGETVKLGYVDQSRD
                     TLDPNKTVWEEISDGKDVIQLGKREVPSRAYVGWFNFRGPDQQKKVGLLSGGERNRVH
                     LAKMLKEGSNLLLLDEPTNDLDTETLSALESALEDFPGCAVIISHDRFFLDRIATHIL
                     AFEGDSHVEWFEGNFQAYLEDKKRRLGDAAVEPHRIKFKRFERKN"
     gene            2552..3508
                     /locus_tag="VFX46_04235"
     CDS             2552..3508
                     /locus_tag="VFX46_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5599826.1"
                     /translation="MAGHIHTHDHSHRHVHMDAAAGDRRVFLAVVVNLGLTIGQLVGG
                     FFAGSLALIADAVHNFSDAISLGIAFFARKIARRQSDERMTFGYVRAELVAALINYTT
                     LIVVGVYLAYEAILRFFFPQEVEGWLVVAVAGLALVIDAITALLTYSLAKTSANIRAA
                     FLHNVADALGSIAVIIGGALILTFGWHIVDPIVTLIIAGYILWLAFTEVGSVARILML
                     GVPPEIDVEELIAAIRKIDGVEDIHHVHVWAIDENRNSLEAHIVVPDDMEPDAKAVKD
                     RIRALVRERFGVTHTTLEIERASECHEFGNAKSIGHHIGHQH"
BASE COUNT          729 a         1127 c         1172 g          648 t
ORIGIN      
        1 gagcgcgtac tggttgccgc tgcccacaat cagcgtagga aagccctcga taccgaactc
       61 ctgggcggta aggaaatcgc ggaacgtctc gttgcgtgcc tcgggggaaa ggagggcagc
      121 cgcaaaagtc tcccgatcct caccttcctc cgcagcgaga tcggccagaa cctcgcggga
      181 ggtcacgtca cgattttccg catagaatgc ggtctggatc cgctccatga aagcgagggc
      241 ccggctcggc tggcgcaggc gcatcgtaac aaccgcgcgg caagcgggct cagtatcgta
      301 aacgaagccc tcgcgctcga agaatgagaa atcgaagggt tgaccggagg cagcactcac
      361 tctcgtccag gcctcgcgga tgtactcctt gtcctgatcg cgcatcggcc gggtctggcc
      421 ggcacgcagc ccgcccatga caagccgcaa aggcaggcgg ccccggaagt gctgctcgat
      481 ggccgagatg accggagcga aaccatagca ccaggagcac atgggatcgg cgaagtagat
      541 gagctcgcgg gtcatggctg gctccttttc gttagctcat cgtaacccgg gatcgctgcg
      601 aaatgccacg gctaaatccg ggcctgggcc ggataaagcg cgacgccaag tagcttgggt
      661 gttggaatgt gaggtcctat ctcttgggcg gggtgcacgg gcgctgcttt cgatgagccc
      721 cgtcagttga gaccagtaac ggtttcgtct agaaccgcac ccaatttccc gtagcagctc
      781 ggtttgagaa ggcgccaaag gacatggcaa cgccgcaata cgtctttcac atgcaccggc
      841 tctccaagac ctatccgggg ggcaagcagg tcttgaagga catctcgctg tccttcctgc
      901 ccggcgccaa gatcggcgtc gtgggcgtga acggtgcagg caagtccacg ctgctcaaga
      961 tcatggccgg cgtgatcgac gactaccagg gcgaggcgtg ggccgctgaa ggcgtgcgcg
     1021 tcggttacct gccgcaggag ccggagcttg atcccaccaa ggacgtgctc ggcaatgcca
     1081 tggaaggcgt cgccgagaag aaggcgctgc tcgaccgcta caccgagctt gccgccaact
     1141 actcggaaga gacggccgag gagatggcgc gcctgcagga cgagatcgac gcgcagaacc
     1201 tgtgggatct cgatcagcag gtggagcagg cgctcgatgc tttgcgctgc ccgccgggcg
     1261 atgccgacgt gacgaagctt tccggcggcg agaagcgccg tgttgcgctc tgccggctgc
     1321 ttttgtcgaa gcccgatatc ctgttgctcg acgagccgac caaccatctc gacgccgaaa
     1381 cggtggcctg gcttgagcgg tacctgaagg aatatccggg ctgcgtcatc ctcgtgacgc
     1441 acgaccgcta cttcctcgac aacatcaccg agtggacgct ggagctcgat cgcggccagg
     1501 gcatccccta caagggcaat tactcgagct ggctcgagca gaaggccaag cgcctcgaga
     1561 tcgagaagaa ccaggaagag gccaagcagc gcaccctcaa gcgcgagctg gagtggatca
     1621 ggtcgagccc gaaggcgcgg caggccaagg ccaaggcgcg tatccaggcc tatgagaaat
     1681 tggccgagga ggcgggccgc gagcagatca gccacgctca gatccagatc ccacctggtc
     1741 cgcgactcgg cagcgtcgtc ctggaggtcg agaacctcac gaagggttac ggcgacaggc
     1801 ttctcatcga gaacctttcg ttcaagcttc cgccaggcgg catcgtcggc gtgatcggcc
     1861 cgaacggcgc cggtaagacg acgctgttcc gaatgatcac gggtcaggaa aagccggatg
     1921 ccggcacgat ccggctcggc gagacggtga agctcggcta cgtggatcag tcacgcgaca
     1981 cgctcgatcc gaacaagacg gtctgggagg agatttcgga cggcaaggac gtcatccagc
     2041 tcggcaagcg cgaggtgccg tcgcgcgcgt atgtcggctg gttcaatttc cgcgggccgg
     2101 accagcagaa gaaggtcggg cttctatccg gcggtgagcg caatcgtgtg catctcgcca
     2161 agatgctgaa ggaaggcagc aatctgctgc tcctcgacga gccgaccaac gacctcgaca
     2221 ctgagacgct gtcggccctc gagtcggcgc tcgaggactt tccgggttgc gcagtgatca
     2281 tcagccacga ccgcttcttc ctcgaccgta tcgccacgca catcctggcc ttcgagggtg
     2341 acagccacgt cgagtggttc gagggcaact tccaggccta tcttgaggac aagaagcgcc
     2401 gcctgggcga cgcggccgtg gagccgcatc ggatcaagtt caagcggttc gagcggaaga
     2461 actgaggtca gtcgccgccg gcttgacccc ggcggaatgc cggaacctgc tctatcggcg
     2521 cctgttccgg ctggagttgg ccggaggcga gatggcagga catatccata cgcacgacca
     2581 cagccacaga cacgtgcaca tggacgcggc ggccggcgac cggcgcgtct tcctcgccgt
     2641 ggtggtcaac ctgggcctga ccattggaca gcttgtcggc gggtttttcg caggcagcct
     2701 cgcgctgatc gccgacgccg tgcacaactt ctctgatgcc atctcgctcg gcatcgcatt
     2761 cttcgcccgc aagattgccc gccgccaaag cgatgagcgc atgaccttcg gctacgtgcg
     2821 agcagagctc gtcgcggcac tgatcaacta cacgacgttg atcgtcgtcg gcgtctatct
     2881 ggcctacgag gcaatcctcc ggttcttctt cccgcaggag gtcgagggct ggcttgtggt
     2941 tgccgtggcc ggtcttgcgc tggtgatcga tgcgattacc gccttgctga cctattcatt
     3001 ggccaagacg agcgcgaaca ttcgcgcggc gttccttcac aacgtcgccg atgctctcgg
     3061 gtcgatcgct gtgatcatcg gtggtgcgct gatcctgacc ttcggctggc acatcgtcga
     3121 cccgatcgtg acgctgatca tcgcgggcta catcttgtgg ctggcattca ccgaagtcgg
     3181 cagcgttgcg cggattctaa tgctcggcgt tccgccagag atcgacgtcg aggaactcat
     3241 agctgccatt cgcaagatcg acggggtcga ggacatccat cacgtccatg tctgggcgat
     3301 cgacgagaac cgaaactcgc tcgaggcgca catcgttgtg ccggatgaca tggagcccga
     3361 tgccaaggcc gtgaaggacc ggatcagggc gcttgtccgc gagcgctttg gcgtgacgca
     3421 cacgacgctc gagatcgagc gcgcctcaga gtgtcacgaa ttcgggaacg cgaaatcgat
     3481 cgggcaccac atcggccatc agcactaggc tgagtgtcta agaaggtgct actgcaactt
     3541 gacgtccgcg cggcctaacg actgagcgcg atcaccagcg cctgcgggtt gccgccgttc
     3601 tcgccgatgt agctctcgaa caggttcttg aggaacgggc tcatccagaa acccattacc
     3661 tgcgcatcgc ggacac
//
LOCUS       DASUMG010000255         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_33375, whole genome shotgun sequence.
ACCESSION   DASUMG010000255 DASUMG010000000
VERSION     DASUMG010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33375"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2150)
                     /locus_tag="VFX46_04240"
     CDS             complement(<1..2150)
                     /locus_tag="VFX46_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX5599827.1"
                     /translation="MDYVGLKTYKEDGSLAGEVRLVGLFTSQAYTQPPQQIPFLRHKV
                     ETVMARSEFAPGSHAAKALANVLATFPRDELFQIDEQLLLNWSREIVDLDLRPRVKVF
                     ARFERFERFVSVLVYIPRDRFSTTVRERIGDILAEVYGGHIRDFTPFFPEGPLVRVHY
                     IIERHKEPRPRIREQDLEAQITEATRTWDDRLGDALSKAGESFAALAPKYREAFPAGY
                     TEFFSVDRAIEDIARIERLSPEQPLAIDLHADKAETRRLRAAIYRFDQPIPLSERVPV
                     LENLGFFVIDERSYRVTPRLDGATREVALHDMVLETADGIPIELALHDRRAEECFLAV
                     CRGDAENDNFNRLVITAGADWREVAALRAYATYLRQIGVPFGFRYIGETLNRHAGVAR
                     DFVELFHTRFDPDRNWTSEQRAETEQRIRKRIEGALGQVASLDEDRILRHYMSLISAT
                     VRTNFFQRDEKGRPPATIAFKLAPELIDGLPEPKPYREIWVYSPRVEGVHLRFAPIAR
                     GGIRWSDRAQDFRTEVLGLAKAQQVKNTVIVPQGAKGGFVPKQLPRSGSRDEIAKEGL
                     AAYRAFVSSMLDITDNIVAGEIAPPDRVIRHDGDDPYLVVAADKGTATFSDFANEISL
                     QRNYWLGDAFASGGSAGYDHKRMGITARGAWECVKRHFREMDIDIEAQPFRVVGVGDM
                     SGDVFGNGMLLSPAICLLAAFDHRDIFIDPSPDAA"
BASE COUNT          401 a          677 c          686 g          397 t
ORIGIN      
        1 gccgcatccg ggctcggatc gatgaagatg tcgcgatgat cgaatgcggc aagcaggcag
       61 atggcgggcg agagcaacat cccattgccg aacacgtcgc ccgacatgtc gccgacgccg
      121 accacgcgga atggctgcgc ctcgatgtcg atgtccattt cgcggaagtg ccgctttacg
      181 cactcccatg caccacgcgc cgtgatcccc atgcgcttgt ggtcgtagcc cgccgaaccg
      241 ccggaggcga aggcatcccc gagccagtag ttgcgttgaa gcgaaatttc gttggcgaaa
      301 tcggagaagg tcgccgtgcc cttgtcagcg gccacgacga gataaggatc atcgccatcg
      361 tggcgtatca cgcgatctgg cggggcgatc tctccggcca cgatattgtc cgtgatgtcg
      421 agcatcgaag agacgaacgc ccgataggca gcgaggcctt ccttcgcgat ttcatcgcgt
      481 gatccggaac gaggcagctg cttgggtacg aagccgccct tggcgccttg cggcacgatc
      541 accgtgttct tgacctgctg cgccttcgcc agccccagca cctcggtgcg gaagtcctgg
      601 gctctgtccg accagcggat gccgccgcgc gcgatgggag caaagcgcag gtgcacgccc
      661 tccacccggg ggctatacac ccagatctcc cggtacggct tcggctcggg cagaccgtcg
      721 atgagctcgg gcgccagctt gaacgcaatc gtcgccggcg gccgcccctt ttcgtcccgc
      781 tggaagaagt tcgtgcgtac ggtcgccgag ataaggctca tgtagtggcg taggatccgg
      841 tcttcgtcga ggctggcgac ctgccccaac gcgccttcta tgcgtttgcg aatgcgttgc
      901 tcggtctcgg cgcgctgctc gctcgtccaa ttgcggtccg gatcgaagcg ggtatggaac
      961 agctcgacga agtcgcgcgc cacgccggcg tgacggttca gcgtctcgcc gatatagcgg
     1021 aagccgaacg gcacgccgat ttgcctgagg taggtggcat aggctctaag cgctgccacc
     1081 tcacgccagt cagctccggc cgtgatgacg aggcggttga agttgtcgtt ctccgcatcg
     1141 ccgcggcaaa cggccaggaa gcattcctct gcccgtctgt cgtgtaaggc cagctctatg
     1201 gggatgccat cggccgtttc aagcaccata tcgtgcagcg ctacctcgcg cgtcgcgcca
     1261 tcaaggcgtg gcgttacgcg gtagctgcgc tcgtcgatga cgaagaagcc caggttctcg
     1321 agcacgggca cccgctcgga cagcggaatg ggctgatcga aacgatagat agcggcacgc
     1381 agccgccgcg tctccgcctt gtccgcatga aggtcgatgg caagcggctg ctcgggactt
     1441 aggcgctcga ttcgagcaat gtcctcgata gcacggtcaa cgctgaagaa ttccgtgtat
     1501 cccgcgggaa atgcttcgcg atacttcggc gccagcgcgg cgaagctctc acctgctttg
     1561 ctgagggcgt cgccaaggcg atcatcccac gtacgggtgg cctcagtaat ctgcgcttcc
     1621 agatcttgct cccggatgcg cggacggggc tctttgtgcc gctcgatgat gtagtggacg
     1681 cgcacgagcg gcccttcggg gaagaagggc gtgaaatccc tgatgtggcc gccatagacc
     1741 tcggcgagga tgtcgccgat ccgctcacgg acggtcgtcg agaagcggtc gcgcgggata
     1801 tacaccagga ccgagacgaa ccgctcaaac cgctcgaatc gggcgaaaac tttgactcgt
     1861 ggccgtagat cgaggtcgac gatctcgcgc gaccagttca gcagaagttg ctcatcgatc
     1921 tggaacagct catcgcgcgg aaaggtggcg agcacattgg cgagtgcctt cgcggcatgg
     1981 ctgccgggcg caaactccga ccgggccatg accgtctcga ccttatggcg caggaaagga
     2041 atttgctgcg gcggctgcgt ataggcctgg gacgtgaaaa gcccgaccaa ccgcacctcg
     2101 cctgcaagcg atccgtcctc cttgtaggtc ttgagcccca cgtaatccat gaaaacccgg
     2161 c
//
LOCUS       DASUMG010000256         2993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_836413, whole genome shotgun sequence.
ACCESSION   DASUMG010000256 DASUMG010000000
VERSION     DASUMG010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836413"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFX46_04245"
     CDS             complement(<1..1148)
                     /locus_tag="VFX46_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599828.1"
                     /translation="MIGRSLQPAGSEIAASAGMGLVALFTATTFLSAFLLFSIQPLFA
                     KMVLPVLGGSPSVWAVALCFFQGALLAGYCYAHLLNRLAATHLTGFVHIAVCGAALLA
                     LPISVPAGWSEPPAGDPYLWQLGLFSVAIGLPFLAVAANAPLLQAWFAATGHPDGRDP
                     YFLYAASNLGSLVALLSYPFLLEPLLGTRALAELWAGGFVALIGAITICFFALRGRTS
                     TPLQPTTPAPILDEKPPSWSRRLGWVGLALVPAALLTAFTTHITTDVASAPLLWVLPL
                     ALYLLTYVIVFRERALIPMRVLLVLHLAAVIVALLQLSQTRHEGWFLTSATGVAVFFT
                     SGLVAHRTLYDLRPAPRYLTEFYLWLSLGGVLGGLFAALIAPRLFSEV"
     gene            complement(1247..1936)
                     /locus_tag="VFX46_04250"
     CDS             complement(1247..1936)
                     /locus_tag="VFX46_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX5599829.1"
                     /translation="MKITWFGHSCFRIETGNSVLLIDPFLKGNPTFEKSGVSWEEATQ
                     GVTHVALTHGHDDHVGDAAEICKLRDATLFAVFELAMHVGNQGAQKLEPMNTGGTVRH
                     ADFDLTFTQAHHSSSSGGVYLGHPCGIVLQTRENKTVYHMGDTDVFSGMEHINELYHP
                     NFGIVPIGDRFTMGARTAAYACKKFFRFSTVFPCHYGTFPIIDQTADAFVAEMAGQNV
                     VVPKVGSPVTV"
     gene            2110..2541
                     /gene="ruvX"
                     /locus_tag="VFX46_04255"
     CDS             2110..2541
                     /gene="ruvX"
                     /locus_tag="VFX46_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5599830.1"
                     /translation="MLGLDLGTKTIGVALSDVTRTIASPAETIARTKFKADAARLIQI
                     AAEQDVGGLVLGLPRNLDGSEGPRAQATRAFARNFNRLSPLPILLWDERWSTMAAERM
                     LIDADRSRKRRGEVIDKVAATIILQSALDRLKAFDGPSGDE"
     gene            2645..>2993
                     /locus_tag="VFX46_04260"
     CDS             2645..>2993
                     /locus_tag="VFX46_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation response protein"
                     /protein_id="HEX5599831.1"
                     /translation="MTDEIPNQSPPFVDINLFTTDAALQEAVEREGADYALEQLAEFG
                     AVCGSAEALERGRLANEYPPRLKTHDPKGRRLDIVEFHPAYHACMARSMAEGLHCSSW
                     EGDQTGSAAAAANV"
BASE COUNT          650 a          896 c          961 g          486 t
ORIGIN      
        1 aaaacttcgg agaagagccg cggcgcgatc agtgccgcga acagcccacc gagtacgccg
       61 ccgagcgaga gccacaaata gaactcggtc agatagcggg gcgctgggcg cagatcgtaa
      121 agcgttctgt gggccaccaa accagacgtg aagaatacgg caacacccgt agcactcgta
      181 aggaaccagc cctcgtgacg tgtctgagaa agctgtagca gggcaacgat cacggcagcc
      241 agatgcagaa cgagcagaac ccgcatcgga atgagcgcac gctcgcgaaa cacgatcaca
      301 taggtcagca ggtaaagtgc gagcggcagt acccacagga gcggtgccga tgcaacatcg
      361 gtggtgatgt gggtggtaaa ggccgtcagc agagccgccg gcacgagagc gagccccacc
      421 caaccgagtc ggcgagacca agaaggtggt ttttcgtcca gaatgggtgc cggagtggtc
      481 ggctgtagcg gggtcgaggt gcgaccccgt agggcgaaaa agcagatcgt gatcgctccg
      541 atcagcgcga cgaagccgcc cgcccatagc tcggccaggg ctcgagtgcc cagaagcggc
      601 tcgagcagga aaggatagct gagaagggca accaggctac cgaggttgct agcggcgtag
      661 aggaagtagg ggtcgcgtcc atcgggatga cccgtggcag caaaccaagc ttgtagcagg
      721 ggagcattcg cagcaacggc cagaaacggc agcccgattg ccacagagaa cagcccgagc
      781 tgccagagat acggatcgcc ggcgggcggc tcggaccaac ctgcaggcac gctgatcggc
      841 agcgcgagca gtgccgcccc gcagaccgca atgtgcacga agccggtgag gtgcgtcgcg
      901 gctaaccggt tgagcaggtg ggcgtagcag tagcccgcga gcagcgcccc ctggaagaag
      961 cacagcgcca ctgcccagac cgaaggggag ccgccgagca ccggcagcac catcttcgcg
     1021 aacagcggtt ggatcgagaa caacagaaac gccgacagga aggtcgtcgc ggtgaagagc
     1081 gcaaccagac ccatccccgc ggacgcggcg atttccgacc cggccggctg caagcttcta
     1141 ccgatcattc acatcctcgc agacaggccg ggccatccat ccgggcagaa accatcgcag
     1201 agtggatcag gctccacccg agcttactca atcacaaaag gtcggcctac accgtgacag
     1261 gtgatcccac cttcggcacc acgacgtttt gcccggccat ttcggcgacg aacgcatctg
     1321 ccgtctgatc gatgatcggg aatgttccgt aatggcaggg gaagacggtg ctgaagcgga
     1381 agaacttctt gcaggcgtag gcggccgtcc gtgcacccat ggtgaagcga tctccaatcg
     1441 gcacgatgcc gaagttcgga tgatagagct cgttgatgtg ctccattccc gaaaacacgt
     1501 ctgtatcgcc catgtggtag acggtcttgt tctcccgtgt ttgcagcacg atgccacacg
     1561 ggtgccccag atacacgccg cccgacgagg aagagtggtg cgcctgcgta aatgtcaggt
     1621 cgaagtcggc gtgacgcacg gtccctccgg tgttcatggg ctcgagcttt tgcgcaccct
     1681 gattgccgac atgcatcgcg agctcgaaca ctgcgaaaag cgtcgcgtcg cgcaatttgc
     1741 agatctcggc ggcgtctccg acatggtcat cgtggccgtg ggtgagggcc acatgcgtca
     1801 ctccttgcgt tgcctcctcc caggacacgc ccgatttctc gaaggtcggg ttgcccttca
     1861 ggaaaggatc gatgaggaga acgctgttgc cggtttcaat tcggaagcag gaatggccga
     1921 accaggtaat cttcatgagg atttccctct gtcacaagcg acgtttccgg tcgggggcag
     1981 tataggtcgg gcttcggatg caacaaccct ttcaatcaga tgtgagcaaa gcttgtggct
     2041 gacaacgctc tcggcgagac aacgacagaa cccgccgatt tcgctgcgcg gctcgccaag
     2101 ggtaagcgct tgctgggtct cgacctcgga acaaaaacga tcggcgtagc gctgtcggac
     2161 gtcacgcgaa ccatcgcatc gccagccgag acgattgccc gtaccaagtt caaggctgac
     2221 gcggcccgtt tgatccagat cgcggcggag caggacgtcg gcggtctcgt cctcgggctt
     2281 ccccgcaatc tcgatggaag cgaggggcca cgggctcagg ccacgcgcgc gttcgcccga
     2341 aatttcaacc gcctttcacc tttaccgatc ctgctttggg atgagcgctg gtcgaccatg
     2401 gccgcggagc gcatgctgat cgatgcagat cgtagccgca agcgcagggg ggaggtcatc
     2461 gacaaggtgg ctgcaaccat tatcctgcag tcggcgctgg atcggttgaa ggcgtttgac
     2521 gggccttccg gagatgaatg aactttgcaa tctcggcgtg tagtgagagt ctgtctcagg
     2581 cgtcgcccag gctacagtga ttccagctcc cgatcgcgaa gcagctcgag cgcagaccgt
     2641 tgccatgaca gacgagatcc ccaatcagtc gccgcccttc gtggacatca atctgttcac
     2701 caccgacgct gcgttgcagg aggcggtaga gcgggaaggt gcggactatg ccctcgagca
     2761 gctggccgag ttcggggcgg tttgcggcag tgccgaagcc ctcgagcggg gacggctggc
     2821 gaacgagtat ccgccgcggc tgaagactca cgatccgaag ggccgccgcc tcgacatcgt
     2881 cgagtttcac cccgcctacc acgcctgcat ggcgcgaagc atggccgaag ggctgcactg
     2941 ctcctcctgg gagggggacc aaaccggcag cgccgccgcg gcggctaacg tag
//
LOCUS       DASUMG010000257         2399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_515885, whole genome shotgun sequence.
ACCESSION   DASUMG010000257 DASUMG010000000
VERSION     DASUMG010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515885"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1973)
                     /locus_tag="VFX46_04265"
     CDS             complement(<1..1973)
                     /locus_tag="VFX46_04265"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEX5599832.1"
                     /translation="MNGALLWIAGLIVALLAALFAVPYAVDWNGYRGVFEEQASRMLD
                     REVRIGGDVSLRLLPVPYVRFEKPRIADAEYGEALFRADSFTMWLDLPSLVQGILEAK
                     QVELERPILRLQVEEDGGGNWRNFRVQEAALPFVPRGVALQSVHINEGVLAVHDVVGQ
                     PLTRIEIANGELSAPALEGPYRVRADVRWNGEVREVRVSTAAPDPDGSTRLKVTVRAP
                     ESGNSYTFDGSAVNLFDRPQFDGNLTAVIPISSNRDGLRPTTVEGDEQPSLPAGSLSR
                     GVFEIRASVSANAVSAKLSEIAFSFEQNGQPQLLTGTAEASWHDRPVVRASLASRWLD
                     LDRIADVAPETNALAAIRNLTASVVEFLPTQAEVAAQLSIDQANLAGDVISDLTVSIE
                     NTGEGLAIKQFQAALPGSSRVELAGSLTGSGTAEAFDGEILLRGGNFNRFISWAARGT
                     PFAEARSDAGFSFSGNLKLGRERLELTNASVDTGPNRLNGDVSYQWDGRRKLVISLET
                     GHADISGVLPGSLGPDLLKANLGALAGADPGSGLSELAAGLAEADIRLRVRADALTDG
                     TRVLHNVDADVAIENGNLSIPNLRASTPQGFKLEIDGTVHDFANRATGSLVGVIEAST
                     PTALAEALNIGAGDLDPGKRQWLAALAPLRLAF"
BASE COUNT          435 a          761 c          768 g          435 t
ORIGIN      
        1 atgaaagcga gacgcagcgg agcaagagcg gcgagccatt gcctcttgcc tgggtcgaga
       61 tcgccggcgc caatgttcaa cgcttcggca agggcggttg gcgtcgaagc ctcgatgacg
      121 ccgaccaggg agccggttgc cctgttggcg aaatcgtgga ccgtgccgtc gatctcgagc
      181 ttgaatcctt gtggagtgga ggcccgcagg ttcgggatgc tgaggttgcc attctctatt
      241 gcaacatccg catcgacgtt gtgcagcacg cgcgtgccgt cggtaagagc atcggcgcgg
      301 acacggaggc ggatgtcagc ttcggcaagg cccgcggcga gctcagatag gccggagcct
      361 gggtcggcac ccgccaaggc accgaggttg gccttcaaca gatcgggacc cagggagccg
      421 ggcaacactc cagaaatgtc tgcgtgcccc gtctcgagcg agatgacgag cttgcggcgt
      481 ccatcccact ggtaggaaac atcaccgttg aggcgatttg ggcccgtatc cacgcttgcg
      541 ttggtcagct cgagccgttc tctgcccagc ttcaggttac cgctgaacga gaatccggca
      601 tcgctgcgcg cttcggcaaa cggagttccc cgcgctgccc aggatatgaa tcggttgaaa
      661 ttgccgcccc tcaacaagat ttcgccgtcg aacgcctcgg cagtgccgga gcctgtcagc
      721 gagcccgcca gctccacgcg ggagctaccg ggaagagcag cctggaactg cttgatcgcc
      781 aggccctcgc ccgtgttttc gatggaaaca gtaagatcgc tgatgacgtc accggccaga
      841 ttggcctggt cgatcgagag ttgggcagca acctccgcct gtgtcggcag gaactcgacc
      901 acacttgcgg tcaagtttcg gatcgctgcg agtgcgttgg tttctggagc gacatcggcg
      961 atacggtcga gatccagcca gcgagacgcc aagctggccc tcaccaccgg gcggtcgtgc
     1021 cagctcgctt cggcggttcc cgtgagcaat tgcggctgcc cattctgctc gaatgagaag
     1081 gcgatttcgc tgagtttggc gctgaccgcg ttggcgctga ctgaggcccg gatctcgaaa
     1141 acgcctctgg agagcgaccc cgcaggaagg gaaggctgct catctccttc gacggtggtc
     1201 ggacgcagtc catccctatt gctgctgatc ggaatgaccg cagtgaggtt tccgtcgaac
     1261 tgtgggcggt caaacaggtt cacggcgctg ccgtcgaacg tgtacgagtt ccccgactcg
     1321 ggcgcacgca cagtaacctt cagacgggtg cttccgtcgg gatcaggtgc cgcagtggac
     1381 acgcgcacct cgcggacctc gccattccac ctgacgtccg cccgcactcg atagggcccc
     1441 tccagggcag gcgccgacag ctcgccgttc gcgatctcga tcctggtcag aggctggccc
     1501 acaacatcat ggacggccag aacgccctcg ttgatatgga ccgattgcag cgccactccc
     1561 cgcggcacga acggcaatgc cgcttcttgt acgcggaagt tgcgccagtt ccctccgccg
     1621 tcctcctcga cctgcagccg caggatgggc cgctccagct ccacctgctt ggcttcgagg
     1681 atgccttgga cgagcgacgg caggtcgagc cacatcgtga agctatcagc ccggaacagg
     1741 gcctcgccgt attcggcatc ggcgatgcgc ggcttctcga accggacata cgggacgggc
     1801 agcaagcgga ggctgacatc gccgccgatc cggacctcgc ggtcgagcat gcgggaggct
     1861 tgctcctcga agacgccgcg gtagccgttc cagtccacgg cgtagggcac cgcgaacagc
     1921 gcagcgagca gcgcgacaat gaggccggcg atccagagga gtgcgccgtt catgctcaac
     1981 ctactccgcg tggtcgtgta aatcgcatca ggtcttatcc ggaccgctgg gcgcgaggac
     2041 cactccggta cagctgctcg gcgtcaaccg gcgcggatgc cctgacgttc cgctttatcg
     2101 cacagctcgc ggaaagttgg gacatctttc cggtttcgac ggcacggaga ggctcaatag
     2161 cctgtgcgtg gctgccgcag gcaccggatt tgccgctttt ggcgtgctat atttaggcaa
     2221 tgcagtcgaa tcgtaagcac caggtaccaa acccggccga gtctgttcgt cccgcggccg
     2281 aatcaggcgg gcgatcccct gcagatggtc tgaccgtgtc gcaacgggct ttgcgggctg
     2341 cagagccgcc ctacctcgcg ggtctcaatg aagcgcagcg cgaagccgtc gaggcgctc
//
LOCUS       DASUMG010000258         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_676611, whole genome shotgun sequence.
ACCESSION   DASUMG010000258 DASUMG010000000
VERSION     DASUMG010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676611"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1288
                     /locus_tag="VFX46_04270"
     CDS             83..1288
                     /locus_tag="VFX46_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX5599833.1"
                     /translation="MIKIRNRFRNLLARPDQWKKIIKAATRMKAAAPFSPVSDHLTET
                     ANFGPNPGALRMFSHLPTALPDQCALLVVLHGCTQSAAGYDFGAGWSTLADRFGFALL
                     LPEQQRSNNPNGCFNWFQSGDIERGHGEAASIRQMVATMVSDHGIDPARVFVTGLSAG
                     GAMTSVMLATYPEVFAGGAIIAGLPYGAATNMQQAFKTMYQCSPRAARAWGELVRKAS
                     RHKGPWPRVSVWHGGADATVIPPNAAEIIKQWSDVHGLPVQPSFEAMVDGYPRQVWVN
                     AAGDELIESYTIPSMAHGTPLATGAADDQCGATGPFLLEVGISSSYHIARFFGLAANR
                     RASESSRRPLSARVPELDGAEGAKASAEPLDGEVLEPNFRPSAKHPPLDIGAVIADAL
                     RNAGLLKSG"
     gene            complement(1641..2699)
                     /locus_tag="VFX46_04275"
     CDS             complement(1641..2699)
                     /locus_tag="VFX46_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011046377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5599834.1"
                     /translation="MATTAQIAIAVIGIDIGKNSFHIVGLDHRGAIVLRQKWSRGQVE
                     ARFANMSPCLIGMEACVGAHHLSRKLKALGHDARLMPARYVRPYSKGQKNDFRDAEAI
                     AEAVQRPTMKFVVTKTADQLDLQALHRVRERLVSQRTGIINQIRAFLLERGIAVRQGQ
                     RFLRVELPRILAAPPDVLSPRMVRVIEGLASDWHRLDERIEGLSSEIEELAKRDAGCE
                     RLMSVPGIGPIISSAMVAAIGTGDSFSKGRDFAAWLGLVPRQISTGDRTILGKISKRG
                     NRYLRVLFVQAAWVVLVKVKPTRWEGHGLRPWIEAAKKRLHHNVLAIALANKLARIAW
                     SVLAHGRAFETNKLQAAA"
BASE COUNT          589 a          939 c          935 g          603 t
ORIGIN      
        1 acgtgccctt gctgcggcgc agcaacgttt cccgccttaa gctgttatgt ctgagcgacc
       61 gcggtaaaga tagaggtaag caatgattaa aattcggaat cgatttcgga acctgctggc
      121 gcggccggat cagtggaaga agatcataaa ggccgcgacg cgcatgaaag cggcggctcc
      181 tttctcgcct gtttccgatc atctgaccga aaccgcgaat ttcgggccaa accctggcgc
      241 gttgcgaatg ttcagccacc tgccaacggc tctgccggac caatgtgcgc tccttgtcgt
      301 tctgcacggc tgcacgcaaa gcgcggccgg ctatgatttc ggggcgggat ggtcgacgct
      361 ggccgaccgc ttcggattcg ctctgcttct tcccgagcag cagcgatcga acaatcccaa
      421 tggctgcttc aactggtttc agagcgggga catagaacgg ggacatggag aggcagcatc
      481 gatccggcag atggtcgcga cgatggtctc ggaccatggc atcgaccctg cccgggtttt
      541 cgtcaccggg ctctcggccg gaggtgcgat gacttcggtc atgctcgcca cctatccgga
      601 agtgttcgca ggcggtgcaa tcatcgccgg acttccgtac ggggcggcaa ccaacatgca
      661 gcaggctttc aagactatgt atcaatgctc gccgcgcgca gcccgcgcat ggggtgagct
      721 ggtgcggaag gcgtctcggc acaaaggccc gtggccgcgc gtctccgtgt ggcatggggg
      781 tgcggatgca accgtcattc cgcccaacgc agctgaaatc atcaagcaat ggtccgacgt
      841 gcatggcctg ccggtccagc cgtccttcga ggcgatggtc gatggctatc cccgccaggt
      901 gtgggttaac gcagcggggg atgagctgat cgagtcgtac actatcccca gcatggcgca
      961 tggaacacca ctcgccacgg gggccgcaga tgatcagtgc ggagctactg gaccatttct
     1021 cctcgaagtc gggatttcct cttcgtacca catcgccagg ttttttggtc ttgcggccaa
     1081 tcgcagggct tcggaatcct cgcggagacc gctcagtgcc cgcgtgccgg aactggatgg
     1141 ggcggagggc gcaaaagcgt cagcggagcc gctcgacggc gaagtgctgg agccgaactt
     1201 ccggccaagc gcgaagcatc cacccctaga tatcggtgcg gtcatcgccg acgcattgag
     1261 aaacgccggt ctcttaaaga gcggctagcg attggaataa tttactcagc cgactgccgg
     1321 atcgcccgcc caaggctctc cggcctttcg tcaccggcac acccgcggac tgacccaatg
     1381 tatggtccgg ccgtgcgtag caagaagatt tcgacgagtc aggggtgagc ggtcttgcat
     1441 caatgtatcc ggcctttgat tggagtgtgg actccgggcc atcatggata tcagcgcgcg
     1501 tgcgatctca ttagcggtca ggcctcaact tggccattcg ggtcaccagt gttcgcatgc
     1561 gccgggaaga ccgaacctcc atctcgtctc atcctctcgc agacctcggc gggcaggtaa
     1621 ttcaatgact cgcgaaacga tcacgcagca gcctgaagct tgttcgtctc gaaggctcga
     1681 ccatgagcaa gaacgctcca cgcgatgcgc gcgagcttgt tggcgagggc gatggcgagc
     1741 acgttgtgat gcagccgctt cttggcggcc tcgatccaag gcctgagccc gtggccttcc
     1801 catctggtcg gtttcacttt tacaagaacg acccatgctg cctgcacgaa caggacgcgc
     1861 aggtagcgat tgccccgctt cgatattttg ccgaggatgg tgcggtcccc ggtcgagatt
     1921 tgcctcggaa ccagccctag ccatgcggca aagtcgcgac ccttggagaa gctgtcgcca
     1981 gtcccgatcg cggcgaccat ggcgctggag atgatcggtc cgatgccggg gacgctcatc
     2041 aggcgttcgc atccggcgtc ccgtttggcc agctcctcga tctcgctcga taaaccctcg
     2101 atgcgctcat ccagccgatg ccagtcagaa gccaacccct caatgacacg caccatgcgg
     2161 ggggaaagca catcgggcgg tgcggcaagg atgcgcggta gctccacccg caggaagcgc
     2221 tgtccctgtc gcaccgcgat gccccgctcc agcaggaagg cgcggatttg attgatgatg
     2281 ccggtgcgct ggctgaccag ccgctcacgg acgcgatgca gagcctgtag gtcgagctga
     2341 tcggcggtct tggtgaccac gaacttcatc gtcggtcgct gtactgcctc ggcaatcgcc
     2401 tcagcgtcac ggaagtcgtt cttctggcct ttggaatagg gtcgcacgta cctcgccggc
     2461 atcagccgcg catcgtgccc cagcgccttg agcttgcgac tgagatgatg cgcaccgacg
     2521 caggcctcca tgccgatcag gcacggggac atattggcaa agcgtgcttc gacctgacca
     2581 cgcgaccact tctgccgcag cacgatcgcg ccgcgatgat ccagaccgac gatgtggaac
     2641 gagttcttac cgatatcgat gccgattacg gcaatcgcaa tctgtgctgt cgtagccatg
     2701 gcgtgctcct tgtcttggcg cccctggcca gtttatcgct ggcggggcag gagcacggcc
     2761 ggaccatcca ttcaaccaaa atttgctgtg atgcacagcg cagttcccat gaatggcgtg
     2821 gtagagtgtg gccctcggcc gaggcgagca tatgcaacgg cgcgagttca tcagcctgtt
     2881 aggaggcgca gatgttgtct ggccgctcga ggcgatgcca cgcatccgca cgcatggttg
     2941 aaggagctaa acgatgacac agcatatcct tctcaagctg gccgccataa cgattactgc
     3001 ggcagccatt ggtgcctcgt cagcatccgc tcagtcgggc gcagcccaga tcaccgtcct
     3061 ttacga
//
LOCUS       DASUMG010000259         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_756652, whole genome shotgun sequence.
ACCESSION   DASUMG010000259 DASUMG010000000
VERSION     DASUMG010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756652"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFX46_04280"
     CDS             <1..682
                     /locus_tag="VFX46_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX5599835.1"
                     /translation="IPANPEIMPRKRPMDPEQRAARRVEIKDPRAGKPSVQRYYVTPS
                     YVTAEPGEAEALDLLMKITGSGATSRLYQKLVATDKLASTAGGWYGGSSMDFGKIAVY
                     AVPADGVSLSNVEAAIDAVLADIVANGVTEAELERAKKAYLAEYIYESDNQATLARRY
                     GWGLVVGRTVEDIEAWPERISKVTREDIQKVAARYFDPRRSVTGTLLPVPPEGVEQTR
                     TSGPNGRS"
     gene            696..2066
                     /locus_tag="VFX46_04285"
     CDS             696..2066
                     /locus_tag="VFX46_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX5599836.1"
                     /translation="MAARLLGSSDLVPTPRFLLALVTALVFGLGATYPTSASAMKIQT
                     VKSPGGIEAWLVEEHSVPLIAMRFAFEGGNSQDPAGKEGVANFLTAMMDEGAGDLTAI
                     QFQERMEEIAMRMGFEDSRDSFYGSFETLTENLDEAVELLRLAINEPRFDPDAMERIR
                     GQLLTSLAFAAKDPDRVAAREWFAAAFPDHPYGRPAVGTEESLATITRDDLEKYRKRV
                     FAKDTLKIAVVGDIDAETLGKVLDRVFGDLPEKADLEPIPFVEPKKGGDVHVIEMPVP
                     QSVATFGLAGIPRKDPDFIPAFVMNHILGGGGFSSRLMEEVREKRGLAYSVYSYLQPY
                     QHTAIFAGGVATKNEEIGQSLEVIRAELQRMATEGPTPEELENAKSYLIGSYPLRFDT
                     NAKIANQLLGIMQDGLGQDYIEKRNSLIEAVTLEDVKRAAKRILETDNLIMTVVGQPK
                     NLKGRS"
     gene            2162..>2522
                     /locus_tag="VFX46_04290"
     CDS             2162..>2522
                     /locus_tag="VFX46_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599837.1"
                     /translation="MVESSGAGSSAATLPYRQDIDGVLEGAIGSYGLGESELRGWLGK
                     LEPALDDLRKDWETGSLAILRIPEETSDIAPAEAAIANLMEGASTVLFFGTGGSSLGG
                     QTLAQLAGWNIPGAADEA"
BASE COUNT          498 a          751 c          820 g          453 t
ORIGIN      
        1 aattcctgcc aatccggaga tcatgccgcg caagcggccg atggatcccg agcagcgggc
       61 cgctcgccgg gtcgagatca aggatccgcg ggcaggcaag ccatcggtgc agcgctacta
      121 cgtaaccccg agctatgtga ccgcggagcc aggcgaggcc gaggcgctcg atctcttgat
      181 gaagatcacc gggtccggag ccacgagccg cctctaccag aagcttgttg ccaccgataa
      241 gctcgcctcg accgcgggtg gctggtatgg cggctccagc atggatttcg gcaagatagc
      301 cgtttacgcc gtcccggccg acggagtctc tctgagcaat gtcgaggccg cgatcgacgc
      361 tgttctcgcc gacattgtcg cgaacggtgt gacagaagcc gagctggagc gggctaagaa
      421 ggcctatctc gccgagtaca tctacgagag cgacaaccag gcgaccttgg cgcgccgtta
      481 tggctggggt ctggtggttg ggcgcacggt cgaggacatc gaggcctggc cggagcgcat
      541 ctccaaggtg acgcgcgagg acatccagaa ggtggcggca cgctacttcg atccgcgtcg
      601 ttcggttaca ggcacgctgc tgccggttcc gccggagggc gtagagcaga cccgaacctc
      661 tgggccgaac ggtcgttctt gagggagttc acgctatggc tgcgcgcttg ctcgggtcgt
      721 ccgaccttgt tccaactccc cgtttccttc tcgcactcgt caccgcgctc gttttcgggc
      781 tcggtgcgac atatcccacg tcagcatccg caatgaaaat acaaaccgtg aagagcccag
      841 gcggcatcga ggcatggctg gtcgaggagc actcagtccc gctgatcgcg atgcgctttg
      901 cgttcgaggg tggcaactcg caggatccgg ccggcaagga aggcgtcgcg aacttcctga
      961 cggccatgat ggacgaggga gccggcgatc tcacagccat tcagttccag gagcgcatgg
     1021 aagaaatcgc catgcgtatg ggattcgagg atagccgcga ttccttctac ggcagcttcg
     1081 agacgctgac cgagaatctt gacgaggccg tcgagctgct acggctcgcc atcaacgagc
     1141 cacgcttcga ccccgacgcg atggagcgca ttcgcgggca gctgctcacc agcctcgcgt
     1201 tcgctgccaa ggaccccgat cgggttgctg cgcgtgagtg gttcgctgct gctttcccgg
     1261 accaccccta tggccggccg gcggttggca ccgaggagag cctggcgacg atcactcgtg
     1321 atgatctgga aaagtaccgc aagcgggtgt tcgcaaagga cacgctgaaa attgccgtag
     1381 tgggcgatat cgacgccgaa acgctgggca aggtgctcga ccgggtcttc ggtgatctgc
     1441 ccgaaaaggc cgacctggag ccgattccgt tcgtcgagcc gaaaaagggc ggtgacgtgc
     1501 acgtgatcga gatgccggta ccgcagtcgg tcgccacatt tgggctcgcc ggcattccac
     1561 gcaaggatcc ggacttcatc cccgcctttg tcatgaacca tattttgggg ggcggcggct
     1621 tctcctcgcg actgatggag gaggtgcgcg agaagcgcgg gctggcctac tcggtctaca
     1681 gctatctgca gccctatcag cacacggcca tattcgccgg tggagttgcg acaaagaacg
     1741 aggagatcgg ccaatcgctg gaggtgatcc gggccgagct gcagcgcatg gcgacagagg
     1801 gtccgactcc agaggagctt gagaatgcga agagctatct catcggctcc tatccgctga
     1861 ggttcgacac caatgccaag attgccaacc agctcttagg gatcatgcag gatggtctcg
     1921 gtcaggacta catcgaaaag cgcaattcgc tgatcgaggc ggtcacgctg gaggacgtca
     1981 aacgggcggc caagcgcatt ctcgagacgg acaacctcat catgaccgtg gtgggtcagc
     2041 cgaagaacct caagggtcgg tcctgagttc ctccttggcc gaggcccgaa gcttggcact
     2101 ggcaagggac tcaggtctaa caatgcgcga tcgctgatca ggcagaagga gacctcgcgg
     2161 catggtggag tcgtcaggcg ccggatcctc cgcagccacc cttccttacc gacaggacat
     2221 tgacggcgtt ctcgagggag caatcggaag ctacggactc ggcgagagcg agctgcgggg
     2281 ctggctcggc aagcttgaac cagcgctaga cgaccttcgt aaggattggg agaccggaag
     2341 cctcgcgatc cttcgcattc ctgaggagac gtccgacatc gcgccagccg aggcggcaat
     2401 cgccaacctt atggaaggcg cgagcacggt tctgtttttc ggtacgggag gctcgagcct
     2461 cggtggtcag acgctcgcgc agcttgccgg ctggaatatc ccgggagctg ccgatgaagc
     2521 tc
//
LOCUS       DASUMG010000260         4768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_998142, whole genome shotgun sequence.
ACCESSION   DASUMG010000260 DASUMG010000000
VERSION     DASUMG010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998142"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFX46_04295"
     CDS             complement(<1..265)
                     /locus_tag="VFX46_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX5599838.1"
                     /translation="MLRKTSTACLITVLALAGTTGCAEAKPGERVRIQNFSLDATEVT
                     IGQFREFASAVGLVTAAEKAGGGYEWDAGWQLRPGWTVYRPYGR"
     gene            complement(306..1361)
                     /locus_tag="VFX46_04300"
     CDS             complement(306..1361)
                     /locus_tag="VFX46_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5599839.1"
                     /translation="MTKKAMSVVDRRSALKAIAATVLAGSAPLAPSSVLAQAANANRG
                     APLMRAIPKTGEQIPAVGLGTFETFDISPGEPRDHVREVLRRFHAAGGRVVDTSPLYG
                     MAEVCVGDFAFELGIADDLFITNKTWTTGEWLSDNSHSEAQFRRSAERLWRKVMDVQQ
                     VHSLENYDQVLHLLRRWKQEGRVRYIGVTQWNREYYDTMERLVRTADLDFIQVNYSIF
                     SRHCEERLLPACVDNGVAVQINMPFEKARLFTCVAGRPVPDFAREFGAETWAQFFLKF
                     ILSHPAVTVVIPATSNPDHLTDNMGALYGELPDKKMRERMVKYMEEIPGFADTLNQPP
                     YPGKNYGGVVTWPFRSA"
     gene            complement(1478..2371)
                     /locus_tag="VFX46_04305"
     CDS             complement(1478..2371)
                     /locus_tag="VFX46_04305"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5599840.1"
                     /translation="MSSRNSGDIWAVSPRPSDHSRFAWDRAIFDTARRGYTDVVEGKI
                     RSQHHLVMVTLSGGARRHQFKTDCGHRYDGPDRAGCASFLPAGCERRLRLEGVAWEWA
                     SIALRPDLLPGDDDGRPLLCDVPTFSNVQDPFLFGLFAELKRIHSQNGRLDLTYCDTM
                     VAALVQYLSRRYGGAKDEANPGKLPAWRLRRITDYVEAHLDEEIRISTLASLVGLSEG
                     HLHRAFKLTCGQTLLQFINERRIHRAMELLARSSMPITTLAHEVGFSSPSYFARVFRA
                     HTGVGPAEYRRSLRDGQAHRT"
     gene            complement(2577..3494)
                     /locus_tag="VFX46_04310"
     CDS             complement(2577..3494)
                     /locus_tag="VFX46_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX5599841.1"
                     /translation="MFLRIDRLQVEMPLPKDPDPAAAAVVNELMGGRFGEMTTLNTYM
                     YQSFNFRMRDKLRPFYALIANIATEELGHIELVGAAVNGLLYGADGNGKISAGDAPFE
                     STFKSTGAGTNYHLNGPHGIMAVNNLGQPWRGDYIFNSGNLILDLLHNFFLECGARTV
                     KLRAYEMTKNPAARMMLGYLFTRGGVHAHAYALALEKLTGVELKKMLPIPRIFDKEIP
                     ECEKFIKEGSHRKLYRFSPEDYKEVAAIWNGPGLDASEILEVVDGPPQGGPQYQMEGV
                     PEAFIPEYHPEELVEIANKLYTAAGGSKA"
     gene            3734..4417
                     /locus_tag="VFX46_04315"
     CDS             3734..4417
                     /locus_tag="VFX46_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5599842.1"
                     /translation="MTHLFDPPPVPALPIAGSDKLFPVHRIYCVGRNYAEHTREMGFD
                     PDKEPPFFFMKAPDTLVTDGKFPYPSATQDVHWEIELVVALKSGGVNIPRAEAMKHVY
                     GYAVGLDMTRRDLQGVAKKMGRPWEVGKSFEKAAPCSPIVPAEKIGHPTTGAIWLDVN
                     GERRQTGDLSQMIWDISSQIEYLSGLWELRAGDLIFTGTPAGVGAVKKGDKLHGHVDG
                     VGDLEVEVI"
     gene            4429..>4768
                     /locus_tag="VFX46_04320"
     CDS             4429..>4768
                     /locus_tag="VFX46_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX5599843.1"
                     /translation="MTHELHLLPASISLAVAERIVDAALAAATRIGTAPMAVAVLDVG
                     GHLVAFKRQDGLAFLRCEIAQSKAWTALAMGTSSRGSRDRLAERPTFQAALSSLSQGR
                     FIPVPGGVLIL"
BASE COUNT          884 a         1454 c         1477 g          953 t
ORIGIN      
        1 cgcgaccgta cgggcggtac acggtccagc cgggacgcag ttgccagcct gcatcccact
       61 catagccgcc tccggctttt tcggctgctg tgacgagccc gacggcgctc gcgaattcgc
      121 ggaattgccc gatcgtgacc tctgtggcgt cgagcgaaaa gttttggatc cgtacacgct
      181 cgccaggctt tgcctcagcg catcccgtcg ttcccgcaag cgctagaacg gtgatgagac
      241 aagccgtcga ggttttgcgc aacattcgct gcctcctctt acgaacgcgg cggaagtccc
      301 ttcactcacg ccgaacggaa tggccaggtc accacaccgc cgtaattctt accgggataa
      361 ggcggttgat tcagcgtgtc ggcgaatcct gggatttctt ccatgtattt caccatacgc
      421 tcccgcatct tcttgtcggg aagctcgcca tacagcgcgc ccatgttgtc agtgaggtga
      481 tcgggattgc tcgtggctgg gatcacgacc gtgacggctg ggtgtgaaag gatgaatttc
      541 aggaagaatt gagcccaggt ctcagccccg aactcgcgcg caaaatccgg caccggccga
      601 ccggcaacgc atgtgaacag gcgcgctttt tcgaacggca tgttgatttg caccgcgacg
      661 ccgttgtcga cgcaagccgg cagaagccgc tcctcgcaat ggcgggaaaa gatgctgtaa
      721 ttcacctgga tgaagtccag gtccgccgtg cgcacgaggc gctccatcgt gtcgtaatac
      781 tcgcggttcc attgcgtgac gccgatgtag cgcacccgcc cttcctgctt ccagcggcgg
      841 agcaggtgca ggacctgatc gtaattctct aggctgtgca cctgctggac gtccatcacc
      901 tttcgccaca gccgctcggc agatctccgg aattgcgcct cggagtggct gttgtcggac
      961 agccattcgc ccgtggtcca ggtcttgttg gtgatgaata ggtcatcggc gatgccaagc
     1021 tcgaaggcga agtcgcccac gcacacttcc gccatgccgt acaaaggcga agtgtcgacg
     1081 acccgcccgc cggcggcgtg gaaccgccgc aagacttcgc gcacatggtc gcgcggctcg
     1141 ccagggctta tgtcgaaggt ttcaaaagtg ccgaggccga cggcgggaat ctgttctccc
     1201 gttttcggaa ttgcccgcat taacggcgcg cctctgttgg cattcgccgc ctgcgccagg
     1261 acgctgctcg gagccagcgg cgcgcttcct gcgagaacgg tcgctgctat cgctttcaat
     1321 gccgatcgcc tgtccacgac gctcatcgcc ttttttgtca tcgcttgttc ctcacgctga
     1381 attaggctcg tgccctcgcg cgtgaggtag caaacggcgc ctcggtctgc gcgcgcgatc
     1441 ctgatgacgt gacgcgattc tgatatggct tacgctgtca ggtgcgatga gcctgcccgt
     1501 cccgaaggct gcggcggtat tcggcggggc cgacgccggt gtgcgcccgg aaaacgcggg
     1561 cgaaatagct ggggctgctg aaaccgacct catgcgccag cgtcgtgatc ggcatggagc
     1621 tgcgggcaag aagctccatt gcccggtgga tgcgtctctc gttgatgaac tgcaggagcg
     1681 tctggccaca ggtcagcttg aaggcgcgat gtaagtggcc ctcggacagc ccgaccagtg
     1741 aggcaagcgt cgatattcgg atctcttcgt cgagatgggc ttccacgtag tcggtaatgc
     1801 ggcgcaggcg ccacgcgggg agcttgccag gattggcttc gtctttcgcg cctccgtagc
     1861 gccggctcag atactgaacc agcgccgcca ccatcgtatc gcagtaagtg agatccagac
     1921 gaccattctg cgaatgaatt cttttcagct ctgcaaacag gccgaaaaga aaagggtctt
     1981 gaacgttgct gaacgtcggc acatcgcaca gtagtggccg gccgtcatca tcacccggaa
     2041 gaagatccgg ccgcagcgca atcgacgccc attcccaagc cacgccctcg agcctcaagc
     2101 gacgttcgca gcctgctggc agaaaagagg cacatcccgc ccgatccggt ccgtcatagc
     2161 ggtggccgca gtcggtcttg aactggtgac gccgtgcgcc cccggagagt gtcaccatta
     2221 ccaggtggtg ctgtgagcgg atcttaccct cgacaacgtc ggtgtagccg cgtcgcgcgg
     2281 tatcgaagat ggctcggtcc cacgcgaacc ggctgtggtc ggaaggccgc ggactgacgg
     2341 cccagatatc gccgctgttg cgcgaactca cggtcgatca actccggtca gttcttggaa
     2401 gtcctcatgg agcacgccga tggtcggcca ataaagctcg tgtgcgagcg cgactttttg
     2461 gccatacgca ccggagcgct cttaacactc catcacgccg aaaccgtgaa atttgaaaac
     2521 agagcgccgc gtcgggaaag ccgatgcggc gcttctgaaa agtagccgga aggtgtttac
     2581 gccttcgatc cacctgcagc cgtgtagagc ttgtttgcga tctcgacgag ttcctcgggg
     2641 tgatactcgg ggatgaaggc ctccggaacg ccttccatct gatattgcgg gccgccctgc
     2701 ggaggaccat cgacgacttc gagaatttcg gaggcgtcca ggccaggacc gttccagatg
     2761 gcggcgactt ccttgtaatc ctctgggctg aagcgataga gcttccggtg gctgccttcc
     2821 ttgatgaact tctcgcactc cggaatttct ttgtcgaaga tgcgggggat aggcagcatc
     2881 ttcttcagct cgaccccagt cagcttttca agcgccagag catatgcgtg ggcgtggacg
     2941 ccgcctctgg tgaagaggta gcccagcatc atgcgcgctg ccgggttctt ggtcatttca
     3001 taggcacgca gcttcaccgt gcgggcgccg cactcgagga agaagttgtg cagcaggtcc
     3061 aggatgagat tgccggagtt gaagatgtag tcgccccgcc agggctggcc gaggttgttc
     3121 acggccatga tcccgtgcgg cccgttgagg tgatagttcg tcccggcgcc ggtgctcttg
     3181 aaggtgctct cgaacggagc atcgccggct gaaatcttgc cgttgccatc agcgccataa
     3241 agcagtccgt tgacggccgc gccgaccaat tcgatatggc cgagttcctc cgtcgcgatg
     3301 ttggcgatca gggcgtagaa cggcctcagc ttgtcccgca tgcggaagtt gaagctctgg
     3361 tacatgtagg tgttcagggt ggtcatctcc ccgaaccgtc cacccatgag ttcgttcacg
     3421 acggccgccg cagcagggtc agggtctttc ggaagcggca tctcaacctg gaggcgatcg
     3481 atccttagaa acatgcgcgt actccttcat gacctctacc tgaactagcc ggcaccggaa
     3541 cgagcacgcc gggccggtgc tactaaacgg agtggcgcgc gcgggagatc cacttcgagg
     3601 cgaaagattg tcagctgagc aaaggatttc agggacgggc gccgacgcac cgcgtcactc
     3661 gccctggcca gagcgatctg ctaatgctcg gcgcggcgac caacccaagc acacgtcaag
     3721 gaaacacatc tcgatgactc acctgttcga tccgccgccg gtgccggcac tccccattgc
     3781 aggctccgac aagctctttc cggtgcaccg catctattgc gtcggccgca actatgcgga
     3841 gcacacccgc gagatggggt tcgaccccga caaggagccg ccgttcttct tcatgaaggc
     3901 gccggacaca ctggtgacgg atggaaagtt cccctacccg tcagccaccc aggacgtgca
     3961 ttgggaaatc gagctggtag tcgcgctgaa gtccggtggc gtgaacatcc cgcgcgcgga
     4021 ggccatgaag catgtctatg gctatgccgt ggggctcgac atgactcgcc gtgacctgca
     4081 gggcgtggcc aagaagatgg gccggccgtg ggaagtcggt aagtcgttcg agaaggccgc
     4141 tccctgctcg cccatcgtgc cggctgaaaa aatcgggcat cccaccaccg gtgccatctg
     4201 gcttgatgtc aatggcgagc ggcggcagac aggcgacctc tcccagatga tctgggatat
     4261 ctcgagccag attgaatatc tgtcagggct ttgggagctg cgcgctggcg atttgatctt
     4321 cactggcacg ccggcgggtg tgggagctgt caagaaaggc gacaagctcc atggccacgt
     4381 cgacggagtt ggcgacctcg aggttgaggt catctgagga cggctgtcgt gacgcacgag
     4441 cttcatctgt tgccggcatc catctcactg gccgtggccg agcgcatcgt ggatgccgcc
     4501 ctcgcggctg cgacccgcat cggtaccgcg ccgatggccg ttgccgtgct ggacgtgggc
     4561 ggccacctcg tcgcgttcaa gcggcaggat ggtttggcgt ttctgcgctg tgagattgct
     4621 cagagcaaag cgtggacggc gctcgccatg ggaacatcaa gccgtggcag tcgcgacagg
     4681 cttgcggagc ggccgacgtt tcaggccgct ctatcttcac tctcgcaggg ccggttcatc
     4741 ccggtgcctg gcggcgtcct catcctcg
//
LOCUS       DASUMG010000261         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_274837, whole genome shotgun sequence.
ACCESSION   DASUMG010000261 DASUMG010000000
VERSION     DASUMG010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274837"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..1975
                     /gene="ltrA"
                     /locus_tag="VFX46_04325"
                     /pseudo
     CDS             486..1975
                     /gene="ltrA"
                     /locus_tag="VFX46_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152765.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(2302..>2471)
                     /locus_tag="VFX46_04330"
     CDS             complement(2302..>2471)
                     /locus_tag="VFX46_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5599844.1"
                     /translation="RRAKQSPQNYPWLAQLLARRPFKVVAFALANKMARVAWALLARG
                     GTYRAPRLAAA"
BASE COUNT          485 a          745 c          802 g          439 t
ORIGIN      
        1 caggtgatgc ggggctgagc catccgacca cgggcatcgc cgcctgctgc ccgcgcgccg
       61 cgggcggcca cgccgcagcc gcgccgccaa gcagcgtgat gaactcgcgc cgcttcatgt
      121 gtggatgggt gcgccgggag accggcggaa ggtagacggt gcgagtccgt cacggtgaag
      181 gcgtagcgag ctacaccggc ctcgagtcat gcgagtgtgg tcgtgaggcc gcaagcgaag
      241 cgttgacagg ggagtgcgta ggctaggtat tgagcggcgt aagacaagtt cggggcgctg
      301 atgttttccg tccaacagaa ggcaagatgg tcagtgtcgt catcgcaaga cacgagccag
      361 ccccgcgtcg tcgcagaccc tggcatgtgc agaagccttc tgcccgggaa ccgggagatc
      421 tccatcgctg tccccaccag tcgtggccgg gaccgcgtcg ggaaggccgg aggccgtagc
      481 cgacgatgag cgcgagggag aagtcggacc tgcccgaagt agcagagaag cgagcgaaca
      541 aagtggcctc ggctgcggcg gagcttgtgg agcgaagggg cggggccaag gagaatgcgg
      601 aactgcaaag cacggtccgg acgcagagcc gggaagccgt gttacaggcg caggcccgca
      661 tacgagaagc cgtcaacagg aacagacagg acaagctgac ggcgctcctg catcacctca
      721 ccatcgatgt tctgcgggca agcttctttg gtttgaagaa gtccgccgcg ccgggggtcg
      781 acgagatgac ctggaccgaa tacgcggaag gccttgaggg aaacctctcg gacctccact
      841 ctcgcgtcca aacgggagcg tatcgagcgc tgccatcgcg ccggacttat attccaaagg
      901 cagatggcag acagcggccg ctcggcatcg ctgcactgga agataagatc gtccaggcgg
      961 cggtggttgc gatcttgacg ccgatctacg aggcggagtt cctggggttc agctacgggt
     1021 tccgaccgaa gcgtagtcag caccaagcgc tggatgcact cgcgttcggg atcggtaggc
     1081 ggcgcatcaa ctgggtgctt gattgcgacg tccaatcctt tttcgacaag gtcagccaat
     1141 cctggctgat ccgcttcatc gaacatcgga ttggcgatcg acgaatcatc cggttgatcg
     1201 ccaagtggct gacagcaggt gtgctcgaag gcgggcactt gatggtgacg gaggagggga
     1261 cgccacaggg tgcggtcatc agcccgctct tggccaatat ctatctccac tacgtctacg
     1321 atttatgggc ccaccagtgg cggcagcgat gcgccacggg cgacgttatt gtcgtgcgct
     1381 atgcggacga caccatcgtc ggctttgagc atcagcacga ggcagagcgg ttccttgccg
     1441 atctcaaggc aaggctcgca cgcttcgggc tcacccttca tcccgacaag acgcggctca
     1501 tcgagtttgg ccgatccgca attgcgaatc gacattctcg gggtctcggc aagccggagt
     1561 cgttcgactt ccttgggttc acgcattatt gcgcgacccg tcggagcggc tcgggcttcg
     1621 tgctcgggag gaaaccggtg gccaagcgaa tgcgtgccaa gcttcgagag atcaaggagc
     1681 agctcatggc catacggcac gaaggaaccg aaaggcaggg ccgctggctc tgccaggttc
     1741 tgcgcggctg gatggcctat tatgctgtgc caatgagcgg ctcagcgatc tcagcattcc
     1801 ggcatcacat gatcgaacgt tggcacggcg ctctgatgcg ccgaagccaa cgacgtcgat
     1861 taacgtggac gagaatgaag aaaatcgctg accggcacct gccgttccca cgcatcctgc
     1921 acccgtggcc tgaaaagcgg ttcctcgtca ctatccaagg tgggagcccg gtgcgttagc
     1981 agcgcccgcc gggatctgtg cggggggcgg agagaaatct ccgtccctac cgcgaccgcc
     2041 cgcggtgcaa gaggaatctg actatcagcg aagcgttcgg gtgcggtcat gtattcggcc
     2101 tttgaatgcg gccgtttggc cgctggccct gatgtgatcc gctgatcggt cccaaacaaa
     2161 accacgcgct ctttcctcgc gtgacccatc acgggttttc cgatcgacgg tctcgaccgt
     2221 ttcgcatcaa cgtcttcgtc accccttgca gttcacgaaa cgcacggacg caacctcatc
     2281 ctcccatcgg ttggcctctt ctcacgccgc cgcgagtcgt ggcgcccgat aggtgcctcc
     2341 cctggccaac agcgcccagg cgacgcgcgc catcttgttg gcaagcgcaa aggccacgac
     2401 cttgaatggc cgccgtgcca acaactgcgc cagccaggga tagttctgcg ggctctgctt
     2461 ggcgcgccgc a
//
LOCUS       DASUMG010000262         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_194914, whole genome shotgun sequence.
ACCESSION   DASUMG010000262 DASUMG010000000
VERSION     DASUMG010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194914"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VFX46_04335"
     CDS             complement(<1..598)
                     /locus_tag="VFX46_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5599845.1"
                     /translation="MFSLTGRGALVTGATGGIGGAIARKLHAAGASVAISGTKMDRLE
                     ALADELKERVHIVPCDLSDRAAVQKLAGEAEAKLGTLDILVNNAGVTKDNLFVRMKDE
                     EWDEVIAVNLTSTFMLCRAALRNMMRRQWGRIINITSISGIIGNPGQGNYAAAKAGMI
                     GMTKSLAREVASRGITANCIAPGFIKTAMTDALTEKQVE"
     gene            complement(605..1552)
                     /gene="fabD"
                     /locus_tag="VFX46_04340"
     CDS             complement(605..1552)
                     /gene="fabD"
                     /locus_tag="VFX46_04340"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5599846.1"
                     /translation="MAVAFVFPGQGSQEVGMGRELAQAYKAAQDVFDEVDNALGEPLS
                     RIIWEGPKDVLTLTENAQPALMAVSMAVIRVLEVEKGFKLADKVAYVAGHSLGEYSAL
                     AAAGAISVSDAARLLRARGKAMQQAVPVGQGAMAALLGVGLDVAEKVAAEAAQGDVCQ
                     IANDNEPTQVVLSGHKTAIDRVAEIGRKHGVRRAIPLPVSAPFHCSLMQPAADVMAEE
                     LAKVEVRRPVVPVVANVLAEPISDPEEIKKRLVEQVTGTVRWRESVLKMSELGVGDFY
                     EIGAGKVLTGLIKRILKDANAVSVGSPADVDEALAALGR"
     gene            1944..2381
                     /gene="rpsF"
                     /locus_tag="VFX46_04345"
     CDS             1944..2381
                     /gene="rpsF"
                     /locus_tag="VFX46_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX5599847.1"
                     /translation="MPLYEHVFLARQEVSPQQVEAMIDEFGQIIKDGGGKVGKTEYWG
                     LKTLAYKIKKSRKAHYALMNIEASPDVVAEMERRMRLSTDVLRFLTLRVDEHETEPSV
                     QMRKGDDRRDGRGGSRGQRAEGASTFRARATAAKTEQEAESEE"
     gene            2385..2633
                     /gene="rpsR"
                     /locus_tag="VFX46_04350"
     CDS             2385..2633
                     /gene="rpsR"
                     /locus_tag="VFX46_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX5599848.1"
                     /translation="MADVSVAGPRRPFQRRRKTCPFSGQNAPKIDYKDVRLLGRFISE
                     RGKIVPSRITAVSAKKQRELARAIKRARFLGLLPYVLK"
     gene            2766..>3303
                     /locus_tag="VFX46_04355"
     CDS             2766..>3303
                     /locus_tag="VFX46_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEX5599849.1"
                     /translation="MPFSLLIGLGAGLVSALLFASASTGTLLGLLVLVFLTPLPVAIT
                     GLGWGWPSAATAAVSAPIVIALLVTPRAALLHLIALGLPTVILSYLTLLNRNVVVDGE
                     EHVEWYPIGRIVAITSVIAGCLATLALLSTATDMEGLRSSLRETLENSFLRQIEAAQP
                     GGQALGEEEMATFTELMVV"
BASE COUNT          583 a         1054 c         1001 g          665 t
ORIGIN      
        1 cctcgacctg tttttcagtc agtgcgtcgg tcatagccgt cttaatgaaa ccgggtgcga
       61 tgcaattggc tgtaatgccg cgagaagcaa cctcgcgagc caatgacttg gtcattccga
      121 tcatgcctgc tttagccgca gcgtaattgc cctgccctgg gttgccgata atgccagaaa
      181 tggacgtgat gttgataatc cgcccccact ggcggcgcat catattacgc agagccgccc
      241 ggcacagcat gaaggtggag gtgagattca cggcgatcac ctcatcccac tcttcgtcct
      301 tcatgcgaac gaaaaggttg tccttggtca cgccggcgtt gttgaccaga atgtccagtg
      361 tgccgagctt ggcctcggcc tcgccggcaa gcttctgaac tgcggcgcga tcactcaggt
      421 cgcacggaac gatgtgaacc cgctctttga gctcatctgc cagggcctcg agccggtcca
      481 tcttcgtgcc agagatcgcg accgatgcgc ctgcggcatg caatttgcgc gcgattgcgc
      541 cgccgatgcc tccggttgca cccgttacga gcgcacctct gccggtcaaa ctgaacattc
      601 cgcatcacct cccgagtgcc gcgagtgcct cgtccacgtc cgcaggcgag ccgacggaga
      661 ccgcattggc gtctttcaaa attcgcttga tgaggcccgt caacacctta cctgcaccga
      721 tctcgtagaa gtcgccaaca ccgagctcac tcatcttcag caccgactcg cgccagcgca
      781 cggtccccgt cacctgctcg acgaggcgct tcttgatctc ttccggatcg ctgatcggct
      841 cggcgaggac attggcaacc accggcacca cgggccggcg cacttcgact ttggccagct
      901 cttcggccat cacatcagcc gcgggctgca tcaaggagca atggaacgga gcgctcaccg
      961 ggagcggtat ggcccggcgc acgccgtgtt tgcggccgat ttcagcgact cgatcaatgg
     1021 cggttttgtg acctgagagc acgacctggg tcggttcgtt gtcgttggcg atttggcaga
     1081 catcgccctg agccgcttcc gccgcgacct tttccgcaac gtcgagtccc actccgagca
     1141 gcgctgccat ggcgccctgc cccaccggca ctgcttgctg catcgccttt ccgcgagccc
     1201 ggaggagtct tgcagcatca ctcacggata tcgccccagc cgccgccagg gccgagtact
     1261 ccccaagtga atgtccggcg acataggcga ccttgtcagc gagcttgaag cctttttcga
     1321 cctccagcac gcgaatcaca gccatggaga cggccatcag ggccggctgg gcattctccg
     1381 tcagggtcag gacgtccttt gggccttccc agatgatgcg cgacagcggc tcgcccaagg
     1441 cgttgtccac ctcgtcgaag acatcttgcg ccgccttgta ggcctgggcg agttcccggc
     1501 ccatgccgac ttcctggcta ccctggccgg ggaagacgaa agccaccgcc atgaatgctg
     1561 ccgcccctgc tcctgcttgc gagtcgtgag taacggatgg cccatacagc aaggccgcgc
     1621 cattcactct tcgccgttcg ttatttcccg cgctttaggc acggctcagc cggtactgtc
     1681 aagctaacgg gatgaaagag gcttgcagtg ctcgccggtt cgcgtataag gagcgcgttc
     1741 cgaagccatt ccggttggag gctgaacgac gggcgtgccc tggttcctgg ggcatgcttc
     1801 gggatcggat cccgtgccgc cggtgtctcc gctctttgag gtcgtctttg aggcctttcg
     1861 taccgctgat tcgcggtttg aaccggcctt aaaaggggct ctgcgccgga atggacaact
     1921 tgagaagaaa ggcaaaaatc ttcatgccac tttacgagca tgttttcctg gcgcgccagg
     1981 aggtctcgcc tcaacaggta gaggcgatga tcgacgagtt cggccagatc atcaaagatg
     2041 gcggcggtaa ggtcgggaag accgagtact gggggctcaa gaccctcgcc tacaagatca
     2101 agaagagccg caaggcccat tatgcgctga tgaacatcga ggcgtcccct gacgtggtgg
     2161 ccgagatgga gcggcgcatg agactgtcca ccgatgtcct gcggtttttg acgctgcgcg
     2221 tcgacgagca tgagaccgag ccgtccgtcc agatgcggaa gggtgacgac cggcgtgacg
     2281 gtcggggcgg ctcacgcggc cagcgcgccg aaggtgcctc cacgttccgt gctcgggcga
     2341 ccgcggccaa gaccgagcaa gaagctgagt cggaggagta agccatggct gacgtatcgg
     2401 ttgccggtcc ccgccgacct tttcagcgcc gccgtaagac gtgcccgttc tccgggcaga
     2461 acgcgccgaa gatcgattac aaggatgtcc gcctgctcgg gcggttcatc tccgagcgcg
     2521 ggaagattgt gccgagccgc ataacagcgg tttcggcgaa gaagcagcgg gagcttgctc
     2581 gggctatcaa gcgggcgcgc ttcctcgggc ttctgcccta tgttttgaag taggctctag
     2641 gagccagcgg cctgcctgcg gaatgcccgc gggcgggacc gtttgagaag gacacgtggt
     2701 tgaggcacgc ctcggataat gctcgaggcg acctctaacc gcccaagtgc gggacagctg
     2761 gtgccatgcc tttttccctc ctgatcggcc ttggagctgg gctcgtctcg gcacttcttt
     2821 ttgcgtctgc gtcaacgggc acgctgctgg gcctgctggt cctcgtcttc ctgacgccgt
     2881 tgcccgtggc catcaccggc ctgggttggg gctggccatc cgccgcgaca gccgcagtct
     2941 cggcacccat cgtcatcgct cttctcgtca ccccgcgcgc ggcgctactc cacttgatcg
     3001 cgctcggact gccgaccgtg atcctctcat atctcacgct tctcaatcgg aacgtggtgg
     3061 tggacgggga ggaacacgtc gagtggtatc cgatcggtcg catcgtcgcg atcacgtccg
     3121 tcatcgcagg atgcctcgcc actttggccc tcctgtccac cgcgactgac atggagggcc
     3181 tgcgttcctc tcttagggaa accctcgaga acagttttct gcggcagatc gaagccgccc
     3241 agcctggagg acaggccctg ggcgaggagg agatggccac gttcaccgag ctgatggtcg
     3301 tga
//
LOCUS       DASUMG010000263         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_756935, whole genome shotgun sequence.
ACCESSION   DASUMG010000263 DASUMG010000000
VERSION     DASUMG010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756935"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..751)
                     /gene="rsmD"
                     /locus_tag="VFX46_04360"
     CDS             complement(161..751)
                     /gene="rsmD"
                     /locus_tag="VFX46_04360"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX5599850.1"
                     /translation="MRVIGGRFRGRPLASPRDPSDLRLRPSSDRVRESVFNILTHGIP
                     DFSLEGAKVLDLFAGTGALGIEGLSRGASFCLFVEQDAEARGLIRRNIEAFGLTGVTR
                     IFRRDATDLGPAGHRNGFTLVFADPPYGKGLAEKALASAAEGGWLADGAIAVIEESDR
                     AELALPPGFTEIDRRTWGSTQVVFARYETQSGARAP"
     gene            complement(862..1569)
                     /locus_tag="VFX46_04365"
                     /pseudo
     CDS             complement(862..1569)
                     /locus_tag="VFX46_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            1761..>2028
                     /locus_tag="VFX46_04370"
     CDS             1761..>2028
                     /locus_tag="VFX46_04370"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5599851.1"
                     /translation="MAPTSNGSHMLIALDEARRAADRGEVPVGAVIVSPEGAVVARAG
                     NRTRELMDPTAHAEILVIREACRAIGSERLTGFSLYVTLEPCAMC"
BASE COUNT          334 a          658 c          654 g          382 t
ORIGIN      
        1 aaaactatcg ggtgagcgcc ttgtgatatc gcgaatgcgt tcgagacgct caggatggcg
       61 atggtgagca cataaagccc ggcctcgatc cagccgctga tgcgctctgc ccggcgatcc
      121 gggtgttggg gtgatggctt ctgcatagct tcctggacgt tcagggcgca cgcgcgccgg
      181 attgggtttc gtagcgggca aatacaactt gcgtgctacc ccacgtgcgt cggtcgatct
      241 ctgtaaaccc tggaggtagc gcaagttcgg ctcgatcgga ttcctcgatc accgcgatcg
      301 cgccgtcggc cagccagccg ccttccgctg cggaggcaag cgccttttcg gcgagcccct
      361 tgccgtacgg cggatcggcg aagaccagcg tgaacccgtt gcggtgtcct gctgggccga
      421 ggtcggtggc gtcgcggcgg aaaatccgcg tcaccccagt cagtccgaaa gcctcgatat
      481 tgcggcgaat gagtccgcgc gcttccgcat cctgctccac gaacaggcag aagctggcgc
      541 cgcgcgacag gccctcgatg ccgagcgcgc ctgtgccggc gaaaagatcg agcaccttgg
      601 cgccttcgag ggagaagtct gggattccgt gggtcagaat gttgaagacg gattcccgca
      661 cgcggtccga cgacggccgc agccgcaggt cactcggatc cctgggcgag gcgagcggcc
      721 ggccacgaaa ccggcctccg atgaccctca tcgtccgtcg tccttgattt cttgcgatcc
      781 ggcgtcagct tcggaagccg ccgacttgcg gcgctgccgg cgtgccttct cctcctcggc
      841 gtcgttgagc cccaggagtt tcgcggtccg gggtccaagc tgctcgatga gcacacgccg
      901 gcgtacaggc tcgacctcac ctggcttcaa gtcatggagc tggaaggggc cgtaggagac
      961 ccggatcagc cggttgacct gaaggcccaa tgctttgagc accttgcgca cctcgcggtt
     1021 cttgccttcc cggagcgcga ccgtcagcca gacgttgctg cccttcaccc gatcgagcgt
     1081 ggcctcgatc gagccatacc gagtcccctc cacctcgatg ccatccttca gcttgtcgag
     1141 ttcggcctgc gtaacttttc cgaatgcgcg cacgcgataa cgccggagcc aaccggtcga
     1201 cggcagctcg aggtgtcgtg ccagtgcgcc gtccgtcgtc agtaggagca gtccctccga
     1261 attgaagtca agccggccaa cggaaatgac ccgcggcatc ccctcgggca ggttctcgaa
     1321 gaccgtcggg cgcccatcag gatcacggtg agtggtgacg aggccccgcg gcttgtagaa
     1381 ccgccaaagc tgaacgggct cgaaagccgg caagggtttg ccgtcgacca gcaccacgtc
     1441 gtcaggccca acgtcgcgcg ctgggctttt cagaacttcg ccattgacgg tcacacggcc
     1501 ctcggcgatc caacgctcgg cttcccggcg ggaacagagg cctgctctcg cgatcgcttt
     1561 ggcgatgcgc atcgtttgtg gcggccggcc ggaagcctgt tgctcgggtt ctgacagctc
     1621 agagctgtcg cggccctgct tcagcggtac aggcttctgc ggcttggccg gctcggggcg
     1681 ttggtttctc gcaagctgct ttgatcggcc cattcggggc tcacgcatga tgagatcgat
     1741 ccggttttga aggtgaactt atggcaccga cctccaatgg aagccacatg ctgatcgcct
     1801 tggatgaggc ccgtcgtgcg gcagatcgcg gcgaggtgcc ggtgggggcg gtgatcgtct
     1861 cgcctgaagg ggccgtggtc gcccgcgccg gcaaccggac ccgggagctg atggacccca
     1921 ctgcgcatgc cgaaatcctc gtcatccgcg aggcctgcag ggccatcgga tcagaacgcc
     1981 ttaccggatt cagtctctac gtcacgctcg agccttgcgc catgtgcg
//
LOCUS       DASUMG010000264         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1159026, whole genome shotgun sequence.
ACCESSION   DASUMG010000264 DASUMG010000000
VERSION     DASUMG010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159026"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFX46_04375"
     CDS             <1..993
                     /locus_tag="VFX46_04375"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5599852.1"
                     /translation="NVGREMLNVLAERRFPADEVYPIASRRSIGTEVSYGDKTLKCLD
                     IEQFDFRKADFCLMSAGAAVSREWAPRIAATGCTVIDNSSAWRYDLDVPLIVPEVNAD
                     AVRGFRKKNIIANPNCSTAQMVVVLKPLHDYATIKRVVVATYQSVSGAGKEAMDELWY
                     QTKGKYVPGQEVEPKKFPKEIAFNVIPHIDVFMEDGYTKEEWKMVAETKKILDPKIKV
                     TATCVRVPVFVGHSEAVHIEFEKPITAEEARNILREAPGVLVVDKREPGGYVTPVEAA
                     GEFATFVSRIREDPTVENGLAMWIVADNLLKGAALNTVQIAETLINRNLLTRAA"
     gene            complement(1070..1675)
                     /gene="leuD"
                     /locus_tag="VFX46_04380"
     CDS             complement(1070..1675)
                     /gene="leuD"
                     /locus_tag="VFX46_04380"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX5599853.1"
                     /translation="MQKFTTLTGVAAPLPLINIDTDMIIPKQFLKTIKRTGLGKALFY
                     DLRYDENGNENPDFVLNKPAYRNAEILVAGDNFGCGSSREHAPWALLDFGIRCIISTS
                     FADIFYNNCFKNGILPIKVKPEELQVLMDDASRGANAKLTIDLEKQEIRRPDGGVITF
                     DIDPFRKHCLLNGLDDIGLTLQKEAAIRAYEEKMAAVRPWA"
     gene            complement(1772..2299)
                     /locus_tag="VFX46_04385"
     CDS             complement(1772..2299)
                     /locus_tag="VFX46_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599854.1"
                     /translation="MARFSKIIARSLGLLLVVAVGISWPTRGFSEPPTPEQYVAHVGG
                     GAKIVPPGQLVLDNKRVYCGQRPTVLDPNLDDYGAAYPGFLILNPRLLARVSTPVKLW
                     IHAHECGHQFRGPDEETADCFAVQRGRREGWLTPQGLEEVCQFITPARSTSMHLSGSH
                     RCQAMRQCYADPRVR"
     gene            complement(2415..2831)
                     /locus_tag="VFX46_04390"
     CDS             complement(2415..2831)
                     /locus_tag="VFX46_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX5599855.1"
                     /translation="MANSSPWRDARSPSLEDLEVLAAEAWERLPQHFRDMCRGLVIRV
                     EDFASEEVLKELGIESPFDLMGLYQGVSLTRKSFSDPSAEPDMIFLYRRAILDYWAEK
                     DETLGDIVTHVLVHEIGHHFGFSDEDMERIEEEAKS"
     gene            3006..>3454
                     /locus_tag="VFX46_04395"
     CDS             3006..>3454
                     /locus_tag="VFX46_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX5599856.1"
                     /translation="MTALHPELLAYAQSLTLDTATAEDLVHEALLRVLQTRLPPEPKR
                     LRPWAFRILKNLFLDLQRRQRVRASYLQSEAQLADERDDGDPVEMLLVRQAYERLAPR
                     DREIICLIDILGFTYSEASEIIGVPVGTVMSRISRARRSMLELIEDS"
BASE COUNT          718 a          972 c         1071 g          693 t
ORIGIN      
        1 aacgtaggcc gcgagatgct gaatgtcctg gcggagcgcc gctttcctgc ggacgaggtt
       61 tacccaatcg cctcgcgtcg ctccattggc accgaggtca gctacggcga caaaacgctc
      121 aaatgcttag acatcgagca attcgacttc agaaaggccg acttctgcct tatgtcggcg
      181 ggggctgccg tttccaggga atgggcaccc cgcattgccg ccaccggctg cacggtcatc
      241 gataactcgt cggcatggcg ttacgacctg gatgtgccgt tgatcgtgcc ggaagtgaac
      301 gccgatgccg taagaggctt caggaagaag aacatcatcg ccaatccgaa ttgctcgacg
      361 gcgcagatgg tcgtcgtgct gaagcctctg cacgattacg cgacgatcaa gcgggtggtc
      421 gttgccacct atcaatccgt ttccggcgcc ggcaaggagg ccatggacga gctttggtac
      481 cagaccaagg gcaagtacgt tcctggccaa gaggtcgagc cgaagaagtt cccgaaggag
      541 atcgcgttca acgtcattcc tcacatcgac gtcttcatgg aggacggcta cacgaaagag
      601 gaatggaaga tggtagccga gaccaagaag atccttgatc cgaagatcaa ggtgacggcc
      661 acgtgcgtac gtgtgccggt cttcgtcggc cactcggaag cggtgcacat cgagttcgag
      721 aagccgataa cggcggaaga ggcgcggaac attctccggg aggcgcccgg tgtgttggtc
      781 gtcgacaagc gcgagccagg tggctacgtg acgccggtcg aggccgctgg cgagttcgcg
      841 acattcgtca gccgcatccg cgaggacccc accgtcgaaa atgggctcgc gatgtggatc
      901 gtcgccgaca acttgctcaa gggtgctgca ctcaatacgg ttcagattgc tgagacgctg
      961 atcaaccgta accttctgac aagagcggcc taaagagctt cacggcgacg agccaagaac
     1021 aaagggcagg cgcttggcct gcccttttgc gtttcggtcg cttccaaact catgcccagg
     1081 ggcgcactgc agccatcttc tcctcgtagg cacggatggc cgcttccttc tgcagcgtca
     1141 atccgatgtc gtcgaggccg ttgagcaggc aatgtttgcg gaaggggtcg atgtcaaaag
     1201 tgatcactcc gccgtctggc ctgcggattt cctgcttctc aaggtctatg gtgagcttgg
     1261 cgttggcgcc gcggctggca tcgtccatca gcacttggag ctcctcgggc ttcaccttga
     1321 tcggcaaaat gccgttcttg aagcagttgt tgtagaagat gtccgcgaaa ctggtcgaga
     1381 tgatgcagcg gatgccgaag tcgagcagcg cccaaggagc atgctcacgg ctggagccac
     1441 acccgaaatt atcgcccgcg actaggattt cggcattccg gtacgccggc ttgttgagga
     1501 cgaaatccgg gttctcgttg ccgttctcgt cgtaccgcag atcgtagaag agcgccttcc
     1561 ccaagccggt ccttttgatc gtcttcagga actgcttggg gatgatcata tcggtgtcga
     1621 tattgatcaa cggcagtggg gcagccacgc cggtcaacgt cgtgaacttc tgcattgttg
     1681 tcctcgggcc cggttcagcc gcccagtgac tgcggggcgg cccaatagtc acctgtcata
     1741 aaggcgagaa ggtgctatcg cgtcaaggga ctcagcggac ccgtgggtcc gcgtagcatt
     1801 ggcgcatggc ctgacagcgg tgtgagccgg agaggtgcat gctcgtgctc cgggcggggg
     1861 taatgaactg gcagacttcc tctaggcctt ggggcgtcag ccaaccctcg cggcgaccgc
     1921 gttgaacggc gaagcagtca gcggtctcct cgtctggtcc gcggaactgg tggccgcact
     1981 catgcgcgtg aatccagagc ttcacgggtg tcgagacgcg cgccaagagg cggggattga
     2041 ggattaggaa tccgggataa gcagcgccat agtcgtcgag gttgggatcg agaaccgtgg
     2101 ggcgctgtcc gcaatagacg cgcttattat caagcacaag ctggccggga gggacgatct
     2161 tcgcgccacc tcccacgtgg gcgacgtact gctctggagt tggcggctcc gagaagccac
     2221 gggtgggcca agagatgccc acagctacta cgagcagaag acctaaagac cgtgcgatga
     2281 ttttcgagaa cctggccatc atccccccac ccagatgctc ggttgtgccc tctagggatg
     2341 gcttcagtta atcagaggta agatcttccg ctacaggtcg gcatgcaatg ggaatgtgaa
     2401 gcatctgcct gtgatcaaga tttcgcttcc tcctcgattc gctccatatc ctcgtccgag
     2461 aagccgaaat gatggccgat ctcgtgcacg agtacgtgag tgacgatgtc cccaagggtt
     2521 tcatctttct cggcccagta atcgagaata gctcggcggt agaggaagat catatcgggc
     2581 tcagctgacg gatcgctgaa gcttttcctg gtgaggctta ctccctggta aaggcccatg
     2641 agatcgaatg ggctttcgat accaagctcc ttcagcacct cttcggaagc gaagtcctcc
     2701 acgcgaatga cgaggcctcg acacatgtcg cggaagtgtt gaggcagtct ctcccaagcc
     2761 tctgcagcca agacttccag gtcttccagg ctgggtgagc gcgcgtcgcg ccaaggactg
     2821 ctgttcgcca tcgatactac cccggggcac cccgccaagg catgtggcgc acgaagcaga
     2881 gccggtcaat gtctcgggag agaccttgcc gagtcgcaac ggggccggca cattggccta
     2941 actggattca gtctgtcggg ggccgaaggg caagtagcgt gaggtggtgg tcgagcagac
     3001 gcgcagtgac agctctgcat ccagagctgc tggcctatgc gcaaagcctg acgctcgaca
     3061 cggcaacagc ggaggacctt gtgcacgagg cgctgttgcg agttctacag accagacttc
     3121 cgccggagcc taagcgtttg cggccctggg cctttcgcat cttgaagaat ttgtttctcg
     3181 acctgcagcg ccggcagagg gtccgcgcaa gctatttgca atccgaggcg cagctggcag
     3241 atgagcgaga cgatggtgat ccggtggaga tgctgctcgt gcgccaagcc tatgaaaggc
     3301 tcgctccgcg ggatcgcgag attatctgcc tcatcgacat ccttggattc acctactcag
     3361 aagcgagcga aatcattgga gtcccagtag gtacggtgat gagccgcata agccgtgcaa
     3421 ggcgttccat gctagagctg atagaagact cgaa
//
LOCUS       DASUMG010000265         2459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1159237, whole genome shotgun sequence.
ACCESSION   DASUMG010000265 DASUMG010000000
VERSION     DASUMG010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159237"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFX46_04400"
     CDS             complement(<1..125)
                     /locus_tag="VFX46_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599857.1"
                     /translation="MTRIDNTRVIRAPRGPDISAKSWLTEAPLRMLMNNLDPEVA"
     gene            complement(252..1784)
                     /gene="hutH"
                     /locus_tag="VFX46_04405"
     CDS             complement(252..1784)
                     /gene="hutH"
                     /locus_tag="VFX46_04405"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX5599858.1"
                     /translation="MTTVAIGLGDIGLDGWRKVYRGAAVRLHPDALPKIAASAEAVAA
                     IVARGEPVYGVNTGFGKLATVRIEAADLEALQRNLVLSHSAGVGPPLPRAITRLMIAL
                     KLASLAQGASGIRRSTVALLEAMIERGLTPVIPSQGSVGASGDLAPLAHMAAAMIGLG
                     EIETSSARMPALEALQQHGLEPLKLGPKEGLALLNGTQFSTAYALAGLIETETLFRTA
                     LVTGALSTDAARGSDAPFDPRIHELRRHRGQIVTAAALRALMAGSEIRASHLKCDERV
                     QDPYCLRCQPQVMGAVLDLLRHAAGVLVTEANGVTDNPLIYPDTGEALSGGNFHGEPV
                     AFAADMMAIAICEIGSLAERRIAMLVDPAVSGLPAFLTAHPGLNSGFMIPQVTAAALV
                     AENRQRAHPASIDTIPTSANQEDHVSMSAHGARRLIPMADNLASIVAIELLVAAQACD
                     FHRPLASSPPLERVRERLRAGVPFLDQDRSFAPDIDVAAALVRSGAVIDAAGSELLPL
                     VD"
     gene            complement(1781..>2459)
                     /locus_tag="VFX46_04410"
     CDS             complement(1781..>2459)
                     /locus_tag="VFX46_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5599859.1"
                     /translation="LPAEAEGDKAAYVDLVCREMIPAIAAEGLADAVDGFCETIAFTA
                     NEIARVFEAARAAGLPVKLHAEQLSNSGGAILAARYQALSADHLEYLDEPGAAAMADS
                     GTVAVLLPGAYYFIRERQAPPVDLLRRYKVPIAVATDCNPGTSPLSSLLLAMNMAATL
                     FRLTVEECLAGVTREAARALGQLSEVGTLEPGKWCNLAIWDIEDPAELVYHIGFNPLC
                     TRVWRGR"
BASE COUNT          451 a          766 c          813 g          429 t
ORIGIN      
        1 tcggcgacct cggggtcgag attgttcatc agcatgcgta gcggggcttc ggtgagccag
       61 ctttttgcgg agatatcagg accacgcggg gcgcggatga cgcgggtgtt gtcgatgcgg
      121 gtcattgcaa ctctcctgaa agactttctt ggccgagccc ctctgctatt gccgctgccg
      181 tctcggtcag ccgaactctg cctcccaact ccccgcggag gcccagcggc gtaatcgctt
      241 gcgccaagct tttagtcgac gagcggcaac agttctgatc cggccgcgtc gattaccgca
      301 ccgctccgca cgagtgctgc agctacgtcg atgtccggag caaaagaacg gtcctggtcc
      361 aggaaaggca ctccggcgcg caggcgctcg cggacgcgct ctaagggcgg gctagaagcg
      421 agcggccggt ggaaatcgca ggcttgtgca gcgacgagca gctcgatagc gacgatgctg
      481 gcgagattgt cagccatcgg gatcagccgg cgggcgccgt gggccgacat cgacacgtgg
      541 tcctcctgat tggccgacgt cggaatggtg tcgatgctgg ccgggtgcgc ccgctgccgg
      601 ttctccgcaa cgagggcggc agccgtgacc tgcggaatca tgaagccgga gttgagcccg
      661 ggatgggcgg tgaggaacgc aggcagcccg gagactgcag ggtcgacaag cattgcgatg
      721 cgccgttccg ccagcgagcc gatctcgcag atggcgatgg ccatcatgtc cgcggcgaaa
      781 gcaacgggct cgccgtggaa attgccgccg gacagtgcct cgccagtgtc agggtagatg
      841 agcggattat ccgtaacgcc attggcttcc gtcaccagaa ctccggccgc gtggcgcagc
      901 aggtcgagca cggcgcccat cacctgtggt tggcagcgca ggcagtaagg atcctggacg
      961 cgttcgtcac acttgaggtg ggaggcgcgg atttcgctcc ctgccatcaa agctcgcaga
     1021 gcagcggccg ttacgatctg tccacgatgc cgacgcaact cgtggatcct tggatcgaac
     1081 ggcgcatcgg agccgcgggc cgcatctgtt gagagggcgc cggtgaccag agccgtgcgg
     1141 aaaagcgtct ccgtttcgat caggcccgcc agcgcatacg cggtcgagaa ctgtgtgccg
     1201 ttaaggaggg ccaatccttc cttgggcccg agcttgagcg gctccaggcc gtgctgctgc
     1261 aacgcttcca gcgcaggcat ccgggccgaa ctcgtttcga tctcgcccag cccaatcatc
     1321 gcggcggcca tgtgggcaag gggagcgaga tcgcctgatg ccccgacgga gccctgcgaa
     1381 ggaatgacgg gcgtcagccc gcgctcgatc atcgcttcca acagcgccac agtcgagcgc
     1441 cggattccgg aggcgccttg agccaagctc gcgagcttaa gtgctatcat caaacgtgtg
     1501 atggcccgcg ggagaggtgg gccgacaccg gcggagtgcg acagaacgag atttcgttgc
     1561 agagcctcga gatcggccgc ttcgatgcgg accgtggcga gctttccgaa ccctgtattg
     1621 acgccgtaga ccggttcgcc gcgcgcaaca atcgccgcga cggcctcagc gctcgccgct
     1681 attttcggca gtgcgtcggg atgcagtcgc acggcggcac cgcgatagac cttgcgccag
     1741 ccgtcgagcc cgatgtcgcc caaaccgatt gctacggtgg tcatcgacct ctccataccc
     1801 gcgtgcacag cgggttgaag ccgatatggt agacgagctc tgccgggtct tcgatgtccc
     1861 aaatggcgag attgcaccac ttgcccggct caagcgtacc gacctcgctg agctggccta
     1921 acgcgcgggc ggcctcccgc gtgacgccgg caaggcattc ctccacagtc aaccggaaca
     1981 gtgtggcggc catgttcatc gccagcagca gtgaggacaa tggcgaggta cccggattgc
     2041 aatccgtcgc aactgcgatc ggcaccttgt agcggcggag cagatcgaca ggcggcgcct
     2101 gcctctcccg gatgaagtag taggcaccgg gaagcagcac tgcgactgtg ccggaatccg
     2161 ccatcgcggc agctccaggc tcgtcaagat attccaggtg gtcagcagaa agcgcctgat
     2221 aacgtgcggc aaggatggcg ccgccactgt tcgaaagctg ctctgcgtgg agcttcaccg
     2281 gcagacctgc ggctctggcc gcttcgaaca cgcgggcgat ttcgtttgcg gtgaatgcga
     2341 tggtctcgca gaagccatcc acggcatctg caaggccctc tgcggcaatg gcgggaatca
     2401 tctcgcggca aacaagatcc acgtaagcgg ctttgtcgcc ttcggcttct gctggcagg
//
LOCUS       DASUMG010000266         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1159425, whole genome shotgun sequence.
ACCESSION   DASUMG010000266 DASUMG010000000
VERSION     DASUMG010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159425"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFX46_04415"
     CDS             <1..528
                     /locus_tag="VFX46_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5599860.1"
                     /translation="FLYLVVVAIILSVIYLLLERISRSPWGRLQRAIRDEENSAAMSG
                     KNVPLRRVESFVLGSFIMGVAGAVYAHYVVSIDYSHFNPLFATFIVWVMVMLGGSGNN
                     KGVILGAFAVWGVWSGAAFMIDVLSPALQAISPDLPSRGAYLRWMLVAVMLALIVLFR
                     PQGIIPERKKVSDFM"
     gene            complement(596..1942)
                     /locus_tag="VFX46_04420"
     CDS             complement(596..1942)
                     /locus_tag="VFX46_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599861.1"
                     /translation="MSDFSLRNARRRDFLKGVGAAGGAMALSVTLPRALWAQSPPPKV
                     PIGQVVAFTGSGAEYGPFFRDAAQLALNQINTAAEQVFGGPIVERLIAEDSNTLPTPA
                     IEAARKLVEADGVPAIICEWSSGVTVAVATSVTIPSGVLQVGNGTTSPLVSVLPEDEG
                     KDLLFRTSPSDALQGVVAADLAAGKIIDGYKFDTAATIYINNPYGQGLSNAFAAAFEK
                     RGGKVLAQVAHPEEVQPTYRSILSAALQDKPGVLVLPTYPAHTAQILKESRDIFGYTS
                     WQFTDANRSSEVLDAVGGKDLAGKLGTVPSADPESQSYKAFAEAYRAATKQDRIPPFT
                     ETTYDAAMVIGLALAKAIADGITDASEITGTALRDRLRPIANPPGEKIIGGSQEEITK
                     ALQLLKEGKEIDYVGAGGSVDFDQNGDVKTPIAVWRYTETGDETVRIVPAEEIASE"
     gene            2335..>2836
                     /locus_tag="VFX46_04425"
     CDS             2335..>2836
                     /locus_tag="VFX46_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599862.1"
                     /translation="MVAFGRRSTSDYPRYDSFDSDEIFAFDEMTPGERVCPPPHRSRM
                     AAALRSILFLLIAVGVGWVLMADRTAWPEWLNVVLDHSTGLIDRAQSQLNENATGVAE
                     GPEPSFDQPSLEQAALDERALPETEGLPPSTEIAAESPEEMERPEEVESREVVTLNAT
                     ETQDDES"
BASE COUNT          497 a          870 c          886 g          583 t
ORIGIN      
        1 ttcctctatc tggtggtcgt ggcgatcatc ctctcggtga tctacctcct gctcgaacgc
       61 atctcgcgtt cgccttgggg acgcctccag cgcgccatcc gcgacgagga gaactcggct
      121 gcgatgagcg gcaagaacgt acccctgcgt cgggtcgagt ccttcgtact gggcagcttc
      181 atcatgggtg tcgcgggcgc cgtctatgcc cattacgtgg tcagcatcga ctacagtcac
      241 ttcaatccgc tctttgccac cttcatcgtc tgggtgatgg tgatgctcgg aggcagcggc
      301 aacaacaagg gtgtcattct cggagccttc gcggtttggg gcgtgtggag cggagcggcc
      361 ttcatgatcg atgtgttgag ccctgcgctc caggccatct cgcccgacct gccatcgcgt
      421 ggcgcttatc tccgctggat gctcgtggcg gtgatgctcg cgctgatcgt gctgttccgc
      481 ccgcagggga ttatcccgga gcgtaagaag gtctccgatt tcatgtaacg gccgacacgg
      541 ccacaagaaa atgggcgccg ctcgggcgcc cattttcgtt acgtaagctg ctcctttatt
      601 cggacgcgat ttcctcggcg gggacgatcc gcaccgtctc gtcgccggtc tcggtgtaac
      661 gccagaccgc tatcggcgtc ttgacgtcgc cattttggtc gaagtcgacc gagccaccgg
      721 cgccgacgta gtcaatctct tttccttcct tgagcaactg cagcgccttc gtgatctcct
      781 cctggctgcc gccgatgatc ttctcgcccg gcgggttggc gatcgggcgc aggcgatcac
      841 gaagcgcggt tccggtgatt tcagacgcat cggtgatccc gtcggcgatc gccttggcca
      901 gcgccagacc gatgaccatt gccgcgtcgt aggtcgtctc cgtgaagggc ggaatacggt
      961 cctgcttggt ggctgcgcga taggcttccg cgaatgcctt gtagctttgc gattcggggt
     1021 ctgccgaagg cactgtcccg agcttgcccg caaggtcctt accgccgacc gcatcgagca
     1081 cttcgctgga ccggttcgcg tcagtgaact gccagctcgt gtacccgaag atgtcgcggc
     1141 tctctttcag gatctgagcc gtgtgggccg gatatgtcgg tagcaccagc acgccgggtt
     1201 tgtcctgaag cgccgcgctc aggatggagc ggtaggtcgg ctgcacctcc tcggggtgtg
     1261 cgacctgggc cagcacctta ccgccacgct tttcgaaggc agcggcaaac gcgttggaca
     1321 ggccctgacc ataggggttg ttgatgtaga tggttgccgc ggtgtcgaac ttgtaaccgt
     1381 cgatgatctt gccagctgcg aggtctgccg cgaccacgcc ctgcagggcg tccgaggggc
     1441 tagtgcggaa cagcaggtcc ttgccttcgt cctcggggag tacggacacc agcggcgatg
     1501 tcgtaccgtt gccgacctgc agcacccctg agggaatcgt aaccgaagtg gccaccgcca
     1561 ccgtcacgcc gctcgaccac tcgcagatga tggccggcac accatcggcc tcgaccagct
     1621 tgcgagctgc ctcgatggca ggtgttggaa gcgtattgga gtcttctgca atcaaccgct
     1681 cgacgatcgg accgccgaag acctgttcgg ccgccgtgtt gatctggttc agggccagct
     1741 gtgccgcatc acggaagaac ggcccgtatt cggcgcctga gccggtaaag gccacgacct
     1801 ggccgattgg aaccttcggc ggcgggctct gagcccaaag tgcgcggggc aacgtgacac
     1861 tcaacgccat cgccccgcca gcggctccga cacctttgag aaaatcgcgg cggcgcgcgt
     1921 tcctaagcga aaaatcggac atctgttcct cccgatgaga tacgcgggaa ccggcctcgc
     1981 tggcagttcc ggcaagcctt ccagccatac cacaatcccg ggccaaagcc atcctgctga
     2041 cgatctactg gcgcaaaaac agctcacttt acctggggtt gcgccggtgc gtgcggctcc
     2101 aggtctcgcc cgtctcaccc gtcgcctatt tggtgaagtg gaccagtgcg tgccgacgga
     2161 ttagcggcag tgcagggata taaagcaatc gattatctac ttggatactt gccggttgga
     2221 gccgtccggc ggggccggcg cgggcgcgac ttcgtcatca tttcgcccgc aaattttaga
     2281 tgcttacgca acagcgggga aagacaccga cgagcgacat ttggagggga agccatggtc
     2341 gcctttggtc ggcgttcgac ctcggactat cccagatacg acagcttcga ttccgacgag
     2401 atctttgcct ttgacgagat gacgccgggc gaacgggttt gccccccgcc gcaccggtca
     2461 cgaatggctg ctgcgctccg cagcattctg ttcctgctta tcgctgtggg tgtcggctgg
     2521 gtgttgatgg ctgatcggac agcctggccg gagtggttga atgtcgttct tgatcactca
     2581 acgggtttga tcgaccgagc tcagtcccag ctcaacgaaa acgccactgg ggtagcggag
     2641 ggaccggaac catcattcga ccagccgtcg ttggagcagg ccgcgctgga cgagcgggcg
     2701 ctgcctgaaa ctgaaggatt acccccttcg accgagattg ccgccgaatc gccggaggaa
     2761 atggaaaggc cggaggaggt ggaaagcagg gaggtggtga ccctcaacgc aacggaaacc
     2821 caagacgacg agtctt
//
LOCUS       DASUMG010000267         3249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_677690, whole genome shotgun sequence.
ACCESSION   DASUMG010000267 DASUMG010000000
VERSION     DASUMG010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677690"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VFX46_04430"
     CDS             complement(<1..1043)
                     /locus_tag="VFX46_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599863.1"
                     /translation="MHAIAHPPAGLFGIAFLQAMRRAMPQLLIATIAAGIATYLAQSW
                     LPPAYVAEARLVVNSPDRAAVDRHIEALNSPANLIATSIELGLQDHPEFNTRAPVQRL
                     FDEFGVSRIWGDDEQAHPNEVLLSAIQSTFEVAAVDREKGEIAIRFSAAEPELAAKFV
                     NRIVERYLQNHEADNPIRVLAWAEEPHQASTPRKGLMAILGMIVMLLLCLGVIAVREA
                     LFNATGRREGDKEKLGLPAFRRFSPTGGRSLISVAAAADRILALPRTDRGCRIMVAGE
                     AKDIEVADEARALADALSSSGRKVVLVRWGLGGGDVNGRKSRQQLLGINDLLEGRATF
                     EEIVTRLPSSSAHT"
     gene            complement(1208..1903)
                     /gene="nthA"
                     /locus_tag="VFX46_04435"
     CDS             complement(1208..1903)
                     /gene="nthA"
                     /locus_tag="VFX46_04435"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX5599864.1"
                     /translation="MPHDGHEHEAGHVHNHGDGQPHRHPFQRDHDADPASPYEVLERA
                     IRELLIEKGVLTAEEISAQVDLMDSRSPALGAKVVARAWVDPDYKARLLADTRSALAE
                     LGIDIGTLADFRTVENTPKVHNAVVCTLCSCYPKMLLGIPPAWYKSLAYRSRMVVDPR
                     GVLREFGLELPDDVEVRVHDSTADLRYLVLPMRPEGTEGWTEEQLAEIVTRDCMIGTA
                     VPEVGRKRPDVMS"
     gene            complement(1893..2213)
                     /locus_tag="VFX46_04440"
     CDS             complement(1893..2213)
                     /locus_tag="VFX46_04440"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX5599865.1"
                     /translation="MTGARFQPGDVVRVDHRLTLGHCRTPFFLRGKTGVIVSVQGVFR
                     DPEKLAYHKPGLPAQVLYKVRFRQTDLWPNYRGRPQDQVEADIYEHWLVKIEGEGRTH
                     SHAA"
     gene            complement(2210..2620)
                     /locus_tag="VFX46_04445"
     CDS             complement(2210..2620)
                     /locus_tag="VFX46_04445"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599866.1"
                     /translation="MTEPMRKTEFRQGAGGRAVHDVGGLDFGPIDRTEHDLALWEKRV
                     DAMLVLLTGPKKSAFKIDALRRVIEDYGQQVYDNTGYYDKWIRAIRNLLVEQEILTRD
                     EIEAKVAEVEQAMKAEGRELAGGTVPWDTTEAPI"
     gene            complement(2675..2750)
                     /locus_tag="VFX46_04450"
     tRNA            complement(2675..2750)
                     /locus_tag="VFX46_04450"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2715..2717),aa:Thr,seq:tgt)
     gene            2940..>3249
                     /locus_tag="VFX46_04455"
     CDS             2940..>3249
                     /locus_tag="VFX46_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599867.1"
                     /translation="MRLHVLLAGLAAAVAFSTVPVASSQAEDRGYRVWEDDSYTSFRT
                     RPRVVSGYFIADPYAYRYEPRGYYPYYDAGYWRPLCGAKVDCVPRAFQPPYWKAWGYP
                     K"
BASE COUNT          613 a         1051 c          942 g          643 t
ORIGIN      
        1 gtatgcgcgg agctgctcgg cagcctcgtc acgatctctt cgaacgtcgc gcgtccctcg
       61 aggagatcat tgatccctaa gagctgttgg cgcgatttcc ggccgttcac gtcgccaccg
      121 ccaagccccc accgaaccag caccaccttg cgcccgctgg agctcaaggc atctgcaagc
      181 gcccgggctt cgtctgcgac ctcgatatcc ttggcctcgc ctgcgaccat gatgcggcac
      241 ccccggtccg tcctgggcag cgcgagaatg cggtctgcag cggcagcgac cgaaatcagg
      301 ctgcgccctc ctgtggggct gaagcgacga aaggcgggca agccaagctt ttccttgtca
      361 ccttcgcggc ggccagtggc gttgaatagt gcctcgcgga cggcgatgac gccgaggcaa
      421 aggagcagca tgacgatcat gcccaggatc gccatgagcc ctttccgggg cgtgctggcc
      481 tggtgcggct cctcggccca tgcgaggacc cttattggat tatccgcctc gtggttttgc
      541 aggtaacgct cgacgatccg attgacgaac tttgctgcca actccggctc cgccgcggaa
      601 aatcgtatag cgatctcacc cttttcccgg tccacggcag cgacctcaaa tgtgctttgg
      661 atcgcagaca gcaaaacctc attcgggtga gcttgctcgt catctcccca gatgcggctg
      721 acaccaaact cgtcgaagag tctctgcacc ggggcacgag tattgaattc cgggtgatcc
      781 tggagaccca attcgatgga tgtggcgatt aggttggcgg ggctattgag cgcttcgatg
      841 tgcctatcaa ccgcggcgcg gtccggggaa ttcacgacca gccgtgcctc cgcaacgtag
      901 gccggaggca gccaggattg agccagatag gtcgcaattc cagccgctat cgtcgcgatc
      961 aacaattgcg gcatcgcacg ccgcatcgcc tgaagaaatg cgatgccgaa caacccggct
     1021 ggcggatgag cgatggcgtg catcagcaga tggcaagcgt cggtggtgaa cgaaatcttt
     1081 gctcatccaa tagatgcaag ccggggcgta accaatttgt taaaatcccg cgaggtttag
     1141 cgcggcgtgc tgcgacgggg tgggaaagct caccctttga cccgctgagg aaactgccca
     1201 ggtgctttca ggacattacg tccggacgct tccggccaac ttcgggcacc gcagtaccaa
     1261 tcatgcagtc gcgcgtgacg atctcggcga gctgctcctc ggtccagcct tccgtccctt
     1321 ccggccgcat cggcaaaacg aggtagcgca gatcagccgt tgagtcgtgc acccgcacct
     1381 cgacgtcatc aggcagctcc aggccgaact cccggagcac cccgcgcgga tcaaccacca
     1441 tccgcgaacg gtaggccagg ctcttgtacc aggccggcgg gatgccgagc agcattttcg
     1501 gataacagga gcacagcgtg cacacgacgg cgttgtgcac cttcggagtg ttttccaccg
     1561 tgcggaaatc ggcgagtgtg ccaatgtcaa tgccgagctc cgcgagcgcg gagcgcgtat
     1621 ccgccaacag gcgcgccttg taatcagggt cgacccaggc ccgcgcaacg accttcgcgc
     1681 caagagccgg cgagcggctg tccatgagat cgacctgagc gctgatctcc tcggccgtca
     1741 atactccctt ctcgatcagc agctcgcgga tcgctctctc aagcacctca tatggcgagg
     1801 cgggatcagc atcgtggtcg cgctggaatg ggtggcggtg gggttggccg tcaccatgat
     1861 tgtgcacgtg gccggcctcg tgctcgtgcc catcatgcgg catggctgtg ggtcctcccc
     1921 tcaccttcga ttttcacgag ccaatgctcg taaatatcgg cctcaacctg atcttgcgga
     1981 cgcccgcgat aattgggcca aagatcggtt tgccggaaac ggaccttata gagcacctgc
     2041 gcgggcaaac ccggtttatg gtaggccagc ttttcgggat cgcgaaaaac accctgcacg
     2101 ctgacaatca cacccgtctt accccgcagg aaaaacgggg tccggcaatg cccgagcgtc
     2161 aacctgtggt cgacccggac gacgtcgccc ggctgaaagc gcgcaccagt caaatgggag
     2221 cctccgtcgt atcccacggc acggtgccgc cggcgagctc gcggccctct gctttcatag
     2281 cctgctcgac ctcagcgacc ttggcctcga tttcgtcccg cgtcaggatc tcctgctcca
     2341 cgagcaaatt gcggatggcg cggatccatt tgtcgtaata accggtgttg tcataaacct
     2401 gctggccgta gtcctcgatg acccggcgta acgcgtcgat cttgaacgcg cttttcttgg
     2461 gcccggtcag caggacaagc atggcgtcga cccgcttttc ccagagcgcc agatcatgct
     2521 cggtccggtc gatcgggccg aaatcaagtc cgccaacatc gtgcaccgcg cggccgccag
     2581 ccccctggcg aaactcggtc ttccgcatcg gctcggtcat cggcgctcct cccattcatt
     2641 ggcgccactt cttacggcat gccggatgaa atgctggtgc cggcagaggg acttgaaccc
     2701 ccgacctacc gcttacaagg cggttgctct accaactgag ctatgccggc aatgccgtgg
     2761 agtgatagcg gcttataccc tgggatggag catctggcca gccgctgcgc gctcttgccc
     2821 tgcgcattca tatttcgtca agcataccac tcgaagcgcc gcgacacagt cagcattcat
     2881 acatacaagc gtgtgctaga tctctagcgc gacctcgcga tttcatctgg agaccagtca
     2941 tgcgtttgca cgtcctattg gccggtttgg cggcagcagt cgccttcagc accgttcctg
     3001 tcgcttcatc gcaggccgaa gatcgtggct accgggtctg ggaggatgat agctacactt
     3061 ccttccgcac ccgacctaga gtcgtctcag gctatttcat cgccgatcct tatgcctacc
     3121 gctacgagcc gcgcggctac tatccctatt acgacgcggg ctactggcga cccctctgcg
     3181 gagccaaggt ggactgcgtg ccgcgagctt ttcagccccc ttactggaag gcatggggct
     3241 atcccaagc
//
LOCUS       DASUMG010000268         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_436594, whole genome shotgun sequence.
ACCESSION   DASUMG010000268 DASUMG010000000
VERSION     DASUMG010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436594"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VFX46_04460"
     CDS             complement(<1..969)
                     /locus_tag="VFX46_04460"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5599868.1"
                     /translation="MRIGNAIVATGILLLATLSGIASAGAQATDVRFVLDFLLQGQQS
                     PFVLGRERGYYSAQKVNLASFDPGRGGADSITKVASGTHDIGFGDLSSLIEYNAKNPG
                     RELIAVVLIYDQAPLSLISLKKANIEKPADLIGRKGGAPSVDATYRLFNVFARVNSID
                     PARVQWTTVQPQVREPLLVRGEIDFAAAWVMTAVPSLLGLGVKREDINVMMLRDHGLD
                     LYSNVVFTTPAFAKQHPDTVRGFAKATIQSWQNAASDPKAAITALKRAEPLSDPAVER
                     GRLKWALEFVATPRVRKSGMGDVDPARLTKHIQIVTEGFQLPRTLAS"
     gene            complement(1161..1931)
                     /locus_tag="VFX46_04465"
     CDS             complement(1161..1931)
                     /locus_tag="VFX46_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5599869.1"
                     /translation="MSTTLEKLSPWLFTIGLFVLWELACRIFNVDTFVLPAPSQIFEA
                     AVRYWSPLLKNSFVTLWTTLAGFALAVAFGIVLGIVVGWSRAIYRGLYPVMIGFNSIP
                     KVAVVPILIIWFGIGVVPAILTAFLISFFPIVVNVATGLATMEPELEDVLRALGAKKL
                     DIMLKVGIPRTQPYLFASFKVAITLAFIGAVMSETIGANAGLGHLMVQAGSNFQMPLV
                     FACLLGLAIEGIAMYAVFAYVESYFTRWAFRSSMSAAA"
BASE COUNT          399 a          605 c          675 g          365 t
ORIGIN      
        1 ggatgccagg gtacgcggaa gctggaatcc ctcggtcacg atctggatgt gtttggtgag
       61 ccgtgctggg tccacatctc ccattcccga tttgcgcact ctcggcgtgg cgacaaactc
      121 gagcgcccat ttcagacggc cccgttcgac cgcgggatcg gagagcggtt cggcccgctt
      181 gagcgctgtg attgcggctt taggatcgct cgccgcgttc tgccaggatt gaatggtagc
      241 ctttgcgaat ccgcgtacgg tatcgggatg ttgtttcgcg aaggcgggcg tcgtgaacac
      301 gacattggag tagagatcga ggccgtgatc gcgcagcatc atcacattga tgtcctcgcg
      361 cttgaccccg agtccgagga gggatggtac cgccgtcatg acccatgctg cggcgaagtc
      421 gatctccccg cgcaccagca gcggttcccg cacctgtggc tgcaccgtgg tccattgcac
      481 gcgtgccgga tcgatgctgt tgacgcgagc aaagacattg aacaggcggt aggtcgcgtc
      541 cacggagggc gcacctccct tgcggccaat gaggtccgcc ggcttctcga tgttggcttt
      601 tttgagcgag atcaggctca gcggcgcctg atcatagatg agcaccaccg caatcagttc
      661 gcgaccggga ttcttcgcgt tgtactcgat gagcgagctc agatcgccaa acccgatgtc
      721 gtgggtgccg ctggcgacct tggtgatgga gtcggcgccg ccgcgaccgg gatcgaagga
      781 tgcgaggttg accttctgtg cgctgtagta gccgcgctcg cggccgagca cgaacggcga
      841 ctgctgacct tgcagcagga aatccaggac gaaccggacg tcggtggctt gcgcgcccgc
      901 tgatgcgatg ccggacaagg tcgccagcag caagatgccg gttgcgacga tcgcgttgcc
      961 tatgcgcatg gttgctcctc gtgctcgtcg gtcatcgctc gcttgagccg ggtattttcc
     1021 cctcgttcac ccgcctcgat gcaagcggag atcaagaagg tcgcaatcgc tggccgccgc
     1081 tcgcttgccg tgcagcgcct ggatgatcga gtccggcggc agtcgagcgg gattggattc
     1141 cgccgtcaag agcgaagcgt tcaagctgcc gcgctcatgg acgagcggaa ggcccagcgc
     1201 gtgaaatagg attcgacgta ggcgaatacg gcgtacatcg cgatgccttc gatcgcgagt
     1261 ccgagcaggc aggcgaacac caacggcatc tggaagttcg atccggcctg caccatcaga
     1321 tggcccaggc cggcattggc gccgatggtc tcggacatca cggcgccgat gaaggcaagg
     1381 gtgatggcga ccttgaacga agcgaacaga tagggctgcg tgcgcggaat gccgaccttc
     1441 agcatgatgt cgagcttctt ggcgccgagc gcgcgcagca cgtcctcgag ctccggctcc
     1501 atggtggcga ggccggtcgc cacattcacc acgatcggaa agaacgagat cagaaacgcg
     1561 gtgaggatcg ccggcaccac gccgatgccg aaccagatga tcaggatcgg gaccaccgcg
     1621 actttcggaa tggagttgaa gccgatcatc acgggataga gcccacgata gatcgcgcgc
     1681 gaccagccca cgacgatgcc gagcacgatg ccgaaggcga cggcgagcgc gaagcccgcg
     1741 agcgtcgtcc aaagcgtgac aaaggagttc ttgaggagcg gcgaccagta cctgaccgct
     1801 gcctcgaaga tctgcgaagg cgcaggcaga acgaaggtat cgacgttgaa aatgcggcag
     1861 gcgagctccc acagcacgaa caggccgatg gtgaacagcc agggcgacag cttctccaga
     1921 gtcgtgctca tgcctttcgc acctgggcga tcttggcgcg cagctcgtgc acgatatcgg
     1981 tgaagcgcgg ctcgaagcag atgtcgagat cgcgcgggcg cggcagatcg accgtcttgg
     2041 tcat
//
LOCUS       DASUMG010000269         3391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_196342, whole genome shotgun sequence.
ACCESSION   DASUMG010000269 DASUMG010000000
VERSION     DASUMG010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196342"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFX46_04470"
     CDS             <1..828
                     /locus_tag="VFX46_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599870.1"
                     /translation="FEGLWERARASVGGSILAAQLALDGIIAFHPGGGTHHGRPERAS
                     GFCYFNDPVFAILTLLDGGITRLLYVDLDAHHGDGVQDAFARDGRVSFFSIHEEGRWP
                     GTGGLHDRLGGRAVNVPVPRGITDAEYWFIVEQLLAPMLDRFRPEAVVVTLGADALAG
                     DPLSAMQLSNHTLWAATELCIERAGAAVVLGGGGYNPWTTARLWAGMWARLSGQPIPP
                     SLPEPAVDVLSALSCDLVDDEDRDPQWFTTVADPPCRIEHVTVRQQVRDIVTSLARD"
     gene            956..3334
                     /locus_tag="VFX46_04475"
     CDS             956..3334
                     /locus_tag="VFX46_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX5599871.1"
                     /translation="MNEHVSEPRPAEGVGSRMPEQGAPQRSFALSFGIDRLGLVALKA
                     PLVSAVVIVLLSVLAIMGVMRLKVDDSLSELFRTDTPEFRQYEEIDRRFPSSEYDVLA
                     VVEGPNLLERPQLQAFADAVIDLQLTDGVDGLVSMLSARGKPDATGYAPPIVPDELPE
                     GAEYDAIIEALRTNDIVSGKFLSDDGQLALIVMSLDRQVVDEQGAKAVIGGILETAER
                     ALEGSGLTVKLTGAPVMQLEIRNAVERDRLIYNGLGFLVGALIAYFFFRRLSLTLIAV
                     LGPTIAILWTLGVVGGLDFRLNLFINVLTPLILVSGFSDSMHLVFSIRRDIMAGVDRV
                     TAARNAVLDVAPACLLTAMNACLALISFAWAESALIRTFGKAALLAVCTSYIAVAVVV
                     PTLAALLIRREKAPVSLPPEQRSDGVGFLTRISDWIIHRVAYRPLPYFLAGLIAVVLT
                     GLAYVQLEPHYRLADQVPDKEHALAATDRLDEKLTGANPVHVMIEWNGGQSLYDSRTF
                     EVIARAHAVLEKEAGLGNVWSVDSLRRWLAEAGDNRIETVKAYIKVLPEHLVRRFIDA
                     DEKAVLVTGRLPDVDASEILPVVQKIDQALEPVRHEFQDYEVSVTGLPAIAARNSATL
                     IWELNIGLVGDMFVIFIFLGIALRSVLSSVTSILPSLFPIFATGALLWLTGEGLQFAS
                     IIAITVAFALAIDSTIHFLNRFRIEETRLAPGPGNATAALIATMHHIGPVIVLTTIVL
                     ALGLGVTVLSHLPSLRLFGRLAAVCLFASLIAQLFILPATIALYRRYFPRKA"
BASE COUNT          533 a         1069 c         1072 g          717 t
ORIGIN      
        1 ttcgaggggc tttgggagcg ggcccgcgcc agcgttggcg gttccatcct tgctgcgcaa
       61 ttggcgctcg acggcatcat agctttccac ccgggtggcg gcacccatca cggccgtccg
      121 gagagagctt ccggcttttg ctatttcaat gatccggtat tcgccatcct caccttgctc
      181 gatggcggca tcacgcgcct tctgtacgtc gatctcgatg cccaccacgg ggatggtgtg
      241 caggatgcct tcgcgcgcga tgggcgcgtt tccttcttct ctatccacga ggaggggcgc
      301 tggcccggta ccggcgggct ccatgaccgc ctcggtggcc gcgccgtcaa tgtgcctgtg
      361 ccacgcggca tcacggacgc cgagtattgg ttcatcgtcg agcagcttct tgcgccgatg
      421 ctggaccgtt tccgccccga ggcggtcgtt gttaccctcg gggccgatgc cttggccggt
      481 gatccgttgt cggcaatgca actcagcaac cacacgctgt gggcggcgac ggagctgtgc
      541 atcgagcgcg ctggcgctgc ggtcgtgctg ggaggcggag gctataaccc gtggacgacg
      601 gcgcgactct gggccggcat gtgggcgcga ctctcgggcc agcccatccc tccatcgctg
      661 ccggaacctg ccgtggacgt gctcagtgcg ctctcatgcg acctggtgga cgacgaggat
      721 cgagacccgc agtggttcac gaccgttgct gatccgccat gtcgcatcga acatgtcaca
      781 gttcggcaac aggtgcggga tatcgtgaca agcctcgctc gggattaact ccctaagcaa
      841 gccgcgaacc cggcgagact tcacttcgaa ttggtccgcg atacttgccc ttggacggca
      901 cgggtggcaa gcatcctgcg ggagcctctt tcgagtcggg gcttttggct gcgccatgaa
      961 cgaacacgtc tccgagccac gccctgcgga aggggtggga tcacgcatgc cggagcaggg
     1021 cgctccgcaa cgctcgtttg cgctcagctt tggtatcgat cggctcgggc tggttgcgct
     1081 gaaggcgccc cttgtgtcgg cagtcgtcat cgtgctgctg agcgtgctgg caatcatggg
     1141 cgtgatgcgg ctcaaggtgg acgactcgct ctccgagctg ttccgcaccg atactcccga
     1201 gttccgccag tacgaggaga tcgaccggcg cttcccctcc agcgaatacg acgtcctcgc
     1261 cgtcgtcgag gggccgaacc tgctggagag gccgcagctt caggcctttg cggatgcggt
     1321 cattgacttg cagctgaccg acggcgtcga cggcctcgtg tccatgttgt ccgcgcgcgg
     1381 caagccggat gcaaccggct atgcgccccc gattgttccc gacgagctgc ccgaaggcgc
     1441 cgagtacgat gcgatcatcg aggcgctgcg cacgaatgat atcgtctcgg gcaagttcct
     1501 ctccgatgac ggccagctgg cgctgatcgt gatgtctctc gaccggcagg tcgtggatga
     1561 gcagggggcg aaggctgtca tcggcggaat tctcgaaacg gcagaacggg cgctcgaggg
     1621 atcgggcctc acggtgaagc tcaccggggc gccggtgatg cagcttgaga tccgcaatgc
     1681 ggtcgagcgg gaccgtctca tctacaatgg gctggggttc ctggtcggtg ctttgattgc
     1741 ctacttcttc ttccgccggc tgtcgctgac gctgatcgcg gtgctcggac cgacaattgc
     1801 gattctgtgg acgctgggtg tcgtcggcgg gctcgacttc cgcctgaacc tgttcatcaa
     1861 cgtgctgacg ccgttgatcc tcgtctcggg cttttccgac tcgatgcacc tggtgttctc
     1921 gatccgccgg gacattatgg caggcgtcga ccgggtaacg gccgcgcgaa acgctgtgct
     1981 cgacgttgcc ccagcgtgcc tgctgacggc aatgaatgcg tgtcttgcgc tcatctcgtt
     2041 cgcctgggcg gaatcggcgc tgattcgcac attcggcaaa gcggcgctgc tggcagtctg
     2101 cacgtcttat atcgccgtgg cggtcgtcgt gccgaccttg gccgcgctgc tcattcggcg
     2161 cgaaaaggcc ccggtatccc tgccaccgga acagaggagc gatggggttg ggttccttac
     2221 ccgcatatcg gattggatca tccatcgcgt ggcttaccgg ccgctgccat acttcctcgc
     2281 cgggctgatc gccgttgtgc tgactgggct cgcctacgtg caactcgagc cgcactacag
     2341 gctcgccgat caagtgccgg acaaggagca cgcactcgcg gccaccgatc gtctcgacga
     2401 gaagctgaca ggagccaacc ccgtccacgt catgatcgag tggaatggtg ggcagtcgct
     2461 ctacgactca aggacttttg aggttatcgc gcgtgcacac gccgtgctgg agaaagaagc
     2521 ggggctcggc aacgtctggt ctgtcgacag cctgcgccgc tggctcgccg aagccggtga
     2581 caaccgcatc gagacggtga aggcctatat caaggtcttg cccgaacacc tcgtccgcag
     2641 gttcatcgac gcagacgaaa aggctgtgct cgtaacaggc cggctgccgg acgtggacgc
     2701 gagcgaaatt cttccggtcg tgcagaagat cgatcaggcg ttggagccgg tccgtcatga
     2761 gttccaggac tatgaagttt ccgtaaccgg tttgcccgcg atcgccgcgc ggaactccgc
     2821 cacgctcatt tgggagctga acatcgggct cgtcggcgac atgttcgtca tcttcatttt
     2881 tctgggcatc gcgctgcgtt cggttctatc cagcgtcacg agcattctgc cgtcgctttt
     2941 cccgatcttc gcgaccggtg cgctgctctg gctcacggga gaagggctgc agttcgcgtc
     3001 catcatcgcc atcactgtgg cgttcgcgct ggcgatcgac tcgacgatac acttcctcaa
     3061 tcgcttccgg atcgaggaga cccggctcgc gcctggcccc ggcaatgcaa cggcggcgct
     3121 catcgccacg atgcatcata tcgggcctgt gatcgttctg acgaccatcg ttctggcact
     3181 ggggctcggc gtaaccgtcc tttcgcacct gccatccttg cggctcttcg ggcggctggc
     3241 cgcagtgtgc cttttcgcat cgctgatcgc gcagcttttc attctgccgg cgacgatcgc
     3301 cctgtatcgc cgctatttcc cgcgcaaggc ctgacgatca gggcttccct catattcgca
     3361 ttttcgaata tgatcccgcc atgggatggc t
//
LOCUS       DASUMG010000270         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_678188, whole genome shotgun sequence.
ACCESSION   DASUMG010000270 DASUMG010000000
VERSION     DASUMG010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678188"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..>2211
                     /locus_tag="VFX46_04480"
     CDS             104..>2211
                     /locus_tag="VFX46_04480"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX5599872.1"
                     /translation="MDLGDWSIRNWRPLAGLTSALLIGLGALPGLLTGYNSYLPALGG
                     PNGLLYDVTLKAAQPWRRNIPTAPVVFVAIDDASLSDPELAALPRALFQPVWARLIDG
                     LLEAGARRIAFDVVFAYAGADFQVGAFTLPDYDRSLIDSLERARDRIVLGRFPSVSPA
                     LPFLKAAGASRIGVLDLQLESDGRVRSTAPIIQLADGRIALGFAALGAGLSVRQASAT
                     QRILMTPYAPMTDTPTYSLATLLACLSSAEGTALIRRFIEGRIVVVGTAVPGEDQHRG
                     PTRFLGGGPPAPPADRCAPHSGLSKRSTPDNAPGALMQVAAIQSAASERPVTLAPTWL
                     RLIAGAGLALLFTLMALRDESALALGERDVSPASVLFVQLARSVAFGLVGPILFGCLF
                     SAVAFIFADIWLPMGYPILVTIFAFATIVGIRSVRHRALFRSLYRTAGRYLPPARLVT
                     LARSGFADPLDGHEREVSILLVDLAGFTAFSNRPDLSASDIVRMANRYFTLMQATIDR
                     YGGCSDKFLGDAVLAFWNGLSDEPDHALKALATASDIIRAVGHAEGPQGDLLAARAIV
                     CSGKVYVGDLGAKQRSNFTIIGPAVNETFRLEKVPDAYGLSLLVAGSAAEMIEASKSS
                     QSIGVLAGNVLVRIDDVELKGFDGLRPIYALVPGDDPGLAEFQAARNALDRHMVCEAL
                     AHLRAVDGGMLQVAAKTLLQR"
BASE COUNT          341 a          696 c          714 g          460 t
ORIGIN      
        1 ccgcgccgtc gaccaggcac tgcgtgaacg cgctgcgcgg ggggcgcaat ccggttgcac
       61 acgggtgggc cgctgacggt catggcacgg taggatcgac attgtggatc tcggcgactg
      121 gagcatccgc aactggcggc cactcgcggg cctgacgagc gccctcctca tcgggttggg
      181 cgcgctccca gggttgctga ccggctacaa cagttatctc ccggcgttgg gcggtcccaa
      241 cggcctgctc tatgacgtga cgctcaaagc cgcgcagcca tggcgccgca acattcccac
      301 cgcgccggtg gtgttcgttg cgattgatga cgcgtcgctt tccgatccgg agcttgcggc
      361 tcttccgagg gccttgtttc aaccggtgtg ggctcgcctg atcgatgggt tgctggaggc
      421 cggagcgcgc cggatcgcct tcgatgtggt gtttgcctat gccggagccg atttccaggt
      481 cggcgccttc acgttgccgg actacgaccg gagcctcatc gacagtctcg aaagggcccg
      541 agatcgcatc gttctcgggc gcttccccag cgtttcgccg gcgcttccgt tcctgaaggc
      601 cgcgggagcg tcacggatcg gagttctgga tctccagctc gaatcggatg ggcgggtgcg
      661 aagcaccgcg ccaattatcc agctcgctga tgggcgcatc gccttgggct tcgccgccct
      721 gggcgccggc ctgagcgttc gccaggcatc tgccactcag cgcattctga tgaccccata
      781 tgccccgatg acggatacgc cgacatacag tctggcgacc ttgctcgctt gcctgtcatc
      841 ggccgaaggc actgcactca tccgtcgatt catcgaaggc cggatcgtgg ttgtgggtac
      901 ggcggtgcct ggcgaagacc agcaccgcgg gccgacgcgt ttcctggggg gcggacctcc
      961 cgctccaccc gcggaccggt gcgcgcccca cagcgggctg tccaaacgtt caactcccga
     1021 caatgcgcca ggggcgctga tgcaagtcgc ggcgatccag tcggcggcaa gtgagcggcc
     1081 cgtgacactg gcgccgacct ggttgcggct catcgccggt gccggacttg cgctcctttt
     1141 caccctcatg gctttgcggg acgaaagcgc gctggcactc ggcgagcgcg acgtctcgcc
     1201 ggcctcggtc ctgttcgtgc aattggcccg gtccgttgca tttggattgg tgggcccgat
     1261 ccttttcggc tgtttgttta gcgccgtggc gttcatattc gccgacatct ggctcccgat
     1321 gggctatccc atcctcgtta cgattttcgc atttgcaacc atcgttggga ttcgttcggt
     1381 ccgccatcga gctctgttca gaagtctgta ccggaccgca ggccgctatc tgccaccggc
     1441 acggctcgtg acactggctc gcagcggctt cgccgacccg ctcgatgggc acgaacggga
     1501 agtttcgatt ctattggtgg acctggctgg atttaccgct ttctccaacc ggcccgatct
     1561 gagtgcgtcc gatatcgttc gaatggcgaa ccggtacttc accttgatgc aggccacgat
     1621 cgaccgttac ggggggtgca gcgacaagtt tctcggagac gcggtgctgg ctttctggaa
     1681 cggcctttcc gatgagccgg accacgcatt gaaggccctt gcgacagcct cggacattat
     1741 cagggccgtc ggtcacgccg agggaccgca aggcgatctc ctcgccgcca gagccattgt
     1801 ctgttccggc aaggtctatg ttggcgatct tggcgcaaag cagcgaagca acttcacgat
     1861 catcggtccg gcagtcaacg agacgtttcg ccttgaaaaa gtgccggatg cttatggtct
     1921 ttcgctgctg gttgcgggct cggcggccga aatgatcgag gcttcgaaat cgtcgcaatc
     1981 gatcggcgtg ctcgcgggca acgttctggt gcgaatagat gacgtggagc tcaaggggtt
     2041 cgatggcctg cggccgattt atgcgttggt gcccggcgat gaccccgggc ttgccgaatt
     2101 ccaggcggca cgcaacgctc ttgaccggca catggtgtgc gaggcgctgg cccatctgag
     2161 agccgtcgat ggcggcatgc tgcaagtcgc ggcgaagacg cttctgcagc g
//
LOCUS       DASUMG010000271         5916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1160597, whole genome shotgun sequence.
ACCESSION   DASUMG010000271 DASUMG010000000
VERSION     DASUMG010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160597"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFX46_04485"
     CDS             complement(<1..246)
                     /locus_tag="VFX46_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="HEX5599873.1"
                     /translation="MRILSGARREWPILLIFLLAAASVWTFIELAGRVVANDTPAIDH
                     QLLQLFRDPEDPTRPIGSPWFREMMRDFTGLGGTGVLT"
     gene            complement(578..1219)
                     /gene="ureG"
                     /locus_tag="VFX46_04490"
     CDS             complement(578..1219)
                     /gene="ureG"
                     /locus_tag="VFX46_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HEX5599874.1"
                     /translation="MMTSPHGPLRVGIGGPVGSGKTTLTEMLCKHFRNTYDICVIAND
                     IYTKEDAEILMRAQALPLERIMGVETGGCPHTAIREDCSINLAAIETMKRKFPNLDLI
                     FIESGGDNLAATFSPELADLTIYVIDVAQGEKIPRKGGPGITQSDLLVINKTDLAPYV
                     GADLEVMERDTRRMRGDKPYVFANLRSGVGVGEIAKFIETYGGLAPTGDSLRS"
     gene            complement(1213..2019)
                     /locus_tag="VFX46_04495"
     CDS             complement(1213..2019)
                     /locus_tag="VFX46_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041577574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HEX5599875.1"
                     /translation="MVRGRSITAIITIITTITTTIIRTNLVGLEPRLGQREVAQKARQ
                     AKAQALLQIWLSPAFPVGAFAYSHGLEAAVDQGWIRDRQTLKAWLADLVEFGSLQNDL
                     IFVACAWRAAATENDENLREIIEFAAALQPSAERFLEATQQGRSFLQQIEASWPPPRP
                     MPAAIQDCSDVVYAVAVGVASALHHIPVADVLRAYAIAFVGNLLSAAIRLSIIGPTES
                     QQLLAQLTPDLIAVAASAETATLEDLGSATWRSDLASLLHETQHTRLFRS"
     gene            complement(1946..2446)
                     /gene="ureE"
                     /locus_tag="VFX46_04500"
     CDS             complement(1946..2446)
                     /gene="ureE"
                     /locus_tag="VFX46_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HEX5599876.1"
                     /translation="MQRATRVIRKAELGSQAPVQSITLDRASRFRRRVAMKTDDGVPF
                     LLDLPEATYLADGDALVLENGDLIKVVAAAEPLLEISADNPHALARIAWHIGNRHTPA
                     EITPDAIYIQPDHVLEEMVIGLGAHVREVMRPFEPEGGAYGGKGPLHNGHHHHHHHHH
                     HHDHSH"
     gene            complement(2454..4169)
                     /gene="ureC"
                     /locus_tag="VFX46_04505"
     CDS             complement(2454..4169)
                     /gene="ureC"
                     /locus_tag="VFX46_04505"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX5599877.1"
                     /translation="MATRVSRSAYAGMYGPTVGDKVRLADTELIIEVEKDFTIYGEEV
                     KFGGGKVIRDGMGQSQIANAQGAVDTVITNALIVDHWGIVKADVGITNGRISGIGKAG
                     NPDIQSGVDIIIGPGTEVIAGEGKILTAGGFDTHIHFICPQQVEEALMSGITSMLGGG
                     TGPAHGTFATTCTPGPWHIEMMLRSADAFPMNIGFAGKGNASKPDALVEMIKAGACAL
                     KLHEDWGTTPAAIDCCLSAADDYDIQVMIHTDTLNESGFVESTIEAFKGRTIHAFHTE
                     GAGGGHAPDIIRVAGLPNVLPSSTNPTRPFTVNTIDEHLDMLMVCHHLSSKIPEDLAF
                     AESRIRRETIAAEDILHDLGALSMMSSDSQAMGRVGEVITRTWQTAHKMKVQRGPLPE
                     DKGKGNDNFRVKRYIAKYTINPAIAHGVSKHIGSVEVGKLADLVLWSPAFFGVKPDCV
                     LKGGVIAAALMGDANASIPTPQPVHYRPMFGAYGGAVGATSLLFVSRAAKEANVREKL
                     GLAKEVVAVENVRSGISKASLVHNDATPEITVDPETYEVWADGELLVCEPAAELPMAQ
                     RYFLF"
     gene            complement(4180..4488)
                     /locus_tag="VFX46_04510"
     CDS             complement(4180..4488)
                     /locus_tag="VFX46_04510"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HEX5599878.1"
                     /translation="MIPGEIIPADGEIELNSGRKTVSLTVANTGDRPIQVGSHYHFYE
                     TNPALSFDRAKARGFRLDIPAGTAVRFEPGQTRQVQLVEIAGNREVCGFRQAVMGKLD
                     "
     gene            complement(4499..5086)
                     /locus_tag="VFX46_04515"
     CDS             complement(4499..5086)
                     /locus_tag="VFX46_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX5599879.1"
                     /translation="MKPVGSRNLLRAVAFFAMVALLTTPVFAHTGVSTHGTFFSGLLH
                     PLSGLDHLAAMISVGLWASVVGGKRVWAWPLAFVSAMVAGGILGFSGVSLPAVEPLIA
                     ASVIVLGLAVTAVLNAPVWIGAAVVAAMAVFHGHAHGTEAPGGDWLWYALGFVAATAA
                     LHVSGIALGHFLRLKWPVRLVGGATAALGVVLLVQ"
     gene            complement(5109..5411)
                     /locus_tag="VFX46_04520"
     CDS             complement(5109..5411)
                     /locus_tag="VFX46_04520"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX5599880.1"
                     /translation="MNLTPREKDKLLIAMAAMVARRRLERGVKLNYPEAVALITDFVL
                     EGARDGRSVSELMRDGAKVITRDQVMPGVAEMIHDVQVEATFPDGTKLVTVHEPIR"
     gene            complement(5432..5914)
                     /locus_tag="VFX46_04525"
     CDS             complement(5432..5914)
                     /locus_tag="VFX46_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HEX5599881.1"
                     /translation="MPQPTILFSGARVTRSIEADVAENASLLIGEATIFGRTASGEQM
                     GFGAFRDKWRIRRDGRLLFAEEVRLEGHLGEMMGRPAIANNGIATALLIHTGPNAEDM
                     RESVRKSLFDCPGVHGVSAWRGMLVMRALANGLSEVQCVLHRAVQALTRAAIPRLWQF
                     "
BASE COUNT         1231 a         1785 c         1723 g         1177 t
ORIGIN      
        1 cgtgagcacg ccggtgccgc ccaatcctgt gaagtctcgc atcatctcgc ggaaccacgg
       61 cgagccgatc gggcgcgtag ggtcctcggg atcgcgaaaa agctggagca gctggtggtc
      121 gattgccggc gtatcgttcg cgacaaccct gcccgcaagc tcgatgaagg tccagaccga
      181 tgccgctgcg agaaggaaaa tgagcagtat gggccactcg cgcctggctc cgctcaagat
      241 gcgcatgagc tggttcccag ggcctggttg gctccctgtt tcatcgggtg ggggtttgtt
      301 catgactcaa ttgatccggt cgttggggtt cggccgaata aacgcgcaag ttataacgtt
      361 ttcttacgag agatctgtcg gatgccggga gccggcttgg gctggcagag tacatcaagc
      421 tgcgcggcgg agtgggcgct atgatcgatg ccggcgttcg cgatgttcga gagctgtgcg
      481 agcaaagctt tcccgcctgg tcaggtctct gtgaactcac gcagaaggaa tttcgctata
      541 tcgacggccc ggtgacgtgg cgtttggaaa agagaaatca cgagcgcagg ctgtcgccgg
      601 ttggcgctag tcccccgtaa gtttcgataa acttggcaat ctccccaacg ccgacgcccg
      661 accgcaggtt agcgaacacg tacggcttgt cgccgcgcat gcgccgagtg tcgcgctcca
      721 tcacctcgag gtcagcgccg acgtatgggg cgagatcggt cttgttgatg acgaggagat
      781 cggactgcgt aattccgggc cctcctttgc gcgggatctt ctcgccttgc gcgacgtcga
      841 tgacatagat cgtgagatcg gccagctccg gcgagaacgt cgcggcgaga ttgtcgccgc
      901 cggactcgat gaagatgaga tcgaggttgg gaaacttccg cttcatcgtc tcaatggccg
      961 caaggttgat cgaacagtcc tcgcgaatgg ccgtatgggg gcagcctccc gtctctacgc
     1021 ccatgatccg ctccagcggc agcgcctggg cgcgcatgag gatttccgcg tcctccttcg
     1081 tatagatgtc gttcgcgatg acgcagatat cgtaagtatt gcggaaatgc ttgcacagca
     1141 tctccgtcaa cgtcgtcttg ccggagccga ccgggccgcc aattccaacg cggagcgggc
     1201 catgaggtga cgtcatgatc ggaatagcct cgtgtgctgc gtttcgtgaa gaagcgaagc
     1261 aagatccgag cgccaggtag cgctgccaag gtcttcaagc gttgccgtct cggcagaagc
     1321 cgcgacagcg atgagatctg gagtgagttg cgcgagcagc tgctgacttt cggtggggcc
     1381 aatgattgat aggcgaattg ctgccgaaag cagattgccg acaaatgcaa ttgcgtatgc
     1441 gcgcaggacg tccgccaccg gaatgtggtg aagggcagag gcaacgccta cggcgacagc
     1501 gtaaacgaca tcgctgcaat cctgaatagc cgccggcatt ggtctcggcg gcggccagga
     1561 ggcctcgatc tgttgaagga atgaacggcc ctgctgggtt gcctcaagaa aacgttcggc
     1621 cgagggctgc agggcggccg caaactcaat tatttctcgg agattttcgt cattttctgt
     1681 ggccgctgct cgccaggcac aggcgacaaa aatcagatca ttttgtagag atccaaattc
     1741 cacgagatct gcgagccacg ccttcaatgt ttgacgatcc cggatccaac cctgatcgac
     1801 ggctgcctcg agaccgtggc tatacgcaaa cgcaccgaca gggaaggcgg gagacagcca
     1861 gatctgcagc agagcctgcg cctttgcctg tcgcgctttt tgcgccacct cgcgttggcc
     1921 taacctgggt tcaagaccga ccaaattagt gcgaatgatc gtggtggtga tggtggtgat
     1981 gatggtgatg atggccgtta tggagcggcc ccttaccacc gtacgcgccg ccctccggct
     2041 cgaagggacg catcacctcc cggacgtgcg caccaagacc aatcaccatc tcttccagga
     2101 catgatcggg ctgaatgtag atggcatcag gcgtgatttc cgctggcgta tggcgattgc
     2161 cgatatgcca cgcgatacgg gccaatgcgt ggggattgtc agcggaaatt tcgagcaacg
     2221 gctcagctgc cgcgactacc ttgatgaggt ccccattctc aaggaccaat gcatccccat
     2281 ccgcaagata tgtcgcctcc ggcaaatcga ggaggaatgg cacaccatca tccgtcttca
     2341 tcgcaactct gcgacggaaa cgggacgcgc gatcgagcgt gatgctctgg acgggggcct
     2401 gcgaaccaag ctcagctttg cgaatgacgc gggttgctcg ctgcatgacg acctcaaaac
     2461 aggaaatagc gctgcgccat cggtaattct gccgccggct cgcaaaccaa gagctcaccg
     2521 tcagcccaca cttcgtaggt ttccggatcg acagtgattt cgggcgtggc gtcgttgtgc
     2581 accaagctcg ctttggaaat gccgctacgg acgttttcaa cggcgaccac ctctttggca
     2641 aggccgagct tctcgcgaac gttggcctcc ttggcagcgc gcgagacgaa cagaagcgac
     2701 gtcgcgccga ctgctccgcc atacgcgccg aacatcggtc tgtaatgcac cggctgaggt
     2761 gtggggatgg aggcattcgc atcgcccatc agcgcggcgg caatcacgcc gcctttgaga
     2821 acgcaatccg gcttcacgcc gaaaaacgcc ggcgaccaaa gaacaagatc agcaagcttg
     2881 ccaacctcca ccgatccaat gtgcttggat acgccgtgcg caattgccgg attgatggtg
     2941 tatttggcaa tgtagcgctt cacccggaaa ttatcgttcc ccttcccttt gtcttccggc
     3001 aacggtccgc gctgcacttt catcttatgg gctgtttgcc aggtgcgcgt gatgacctcg
     3061 ccgacgcggc ccatagcctg gctgtccgag gacatcatcg aaagcgcgcc gagatcgtgc
     3121 agaatatcct cggcagcaat ggtttcgcga cggatgcggc tttcggcgaa ggctaaatcc
     3181 tccggaattt tcgacgacag atgatggcag accatcagca tgtcgaggtg ctcgtcgatg
     3241 gtgttcaccg tgaacggccg ggtgggattt gtcgatgacg gcagcacgtt gggaagcccg
     3301 gcaacgcgaa tgatatccgg agcgtgccct ccaccagcgc cttccgtgtg gaaggcgtga
     3361 attgtccggc ctttgaaagc ttcgatcgtg ctctcgacga atcccgattc gttgagcgtg
     3421 tcggtgtgga tcatgacctg aatgtcgtaa tcgtcggcgg ccgagaggca gcaatcgatg
     3481 gctgccggag tggtgcccca atcctcgtga agcttcaggg cgcacgcacc cgccttgatc
     3541 atttcgacga gcgcatcagg cttcgacgca ttaccctttc cggcaaagcc aatgttcatg
     3601 ggaaacgcat cggctgacct gagcatcatt tcgatgtgcc acggccccgg cgtgcaggtg
     3661 gtcgcaaagg tgccgtgcgc aggccccgta ccacctccca gcatcgacgt gatgccggac
     3721 atcagcgcct cctccacctg ctggggacag atgaaatgga tgtgcgtatc gaagccccct
     3781 gcggtcagaa ttttgccctc accggcgatc acctctgtgc cagggccgat gataatatcg
     3841 acgccggatt ggatatctgg attaccggcc ttcccaattc cggaaatccg cccattggta
     3901 atgccaacgt ccgctttcac gatgccccaa tggtcgacga tcagcgcgtt ggtgatgaca
     3961 gtgtcgacgg cgccctgagc attggcgatt tgtgactgtc ccatgccatc gcgaatgacc
     4021 tttccgccgc cgaacttcac ctcctccccg taaatggtga aatccttctc gacctcgatg
     4081 atgagctcgg tgtccgctag gcgcaccttg tcgccgactg ttgggccata catgccggcg
     4141 taggcagagc gcgaaactct tgttgccatc tgtctgcccc tagtcgagct ttcccatcac
     4201 cgcctgccgg aagccgcaga cctcgcgatt gcctgcaatc tccacgagct gcacctgccg
     4261 tgtctgtccg ggctcaaagc gcaccgcggt tccagcggga atatcgagcc ggaaacctcg
     4321 cgcttttgcg cgatcgaaag acaaagccgg attcgtttcg tagaaatgat aatggctgcc
     4381 gacttggata gggcggtcgc cagtattggc gaccgttagg gaaaccgtct tgcggccgga
     4441 attgagctca atctcgccgt cggccgggat gatctcgcca gggatcatcg ctcaccccct
     4501 attgtacgag cagaacgacg cccagggcgg ccgtcgcgcc accaacaagc cgcaccggcc
     4561 acttgagccg aagaaaatgt ccgagcgcaa tgccgctcac gtggagcgca gctgttgcgg
     4621 ccacgaaacc aagagcatac caaagccaat cgccaccagg tgcctccgta ccgtgggcat
     4681 ggccatggaa caccgccatt gccgcaacga ctgctgcgcc aatccacacc ggcgcattca
     4741 gtacagctgt gacagccaga ccgagcacaa tcacggatgc tgcgatgaga ggctctactg
     4801 cgggcagcga aacaccggaa aatcctagaa tgccgcccgc aaccatggcg ctgacaaacg
     4861 ccagaggcca cgcccaaacc cgctttccgc ccactacgga ggcccacaaa ccaacagaga
     4921 tcatcgcggc cagatgatca agtccgctga gtggatggag aaggccgcta aagaaagttc
     4981 cgtgtgtgct gactccggtg tgcgcgaata ccggtgttgt cagcaatgcg accatcgcaa
     5041 agaaggcgac cgcgcgaagc aaattccgac ttccgactgg cttcatatcc aatccccatc
     5101 cctaagattc agcgaattgg ttcgtgtacg gtgaccagct tggtgccatc gggaaatgtc
     5161 gcctcgacct gtacgtcgtg gatcatttcg gcgacgccgg gcatgacttg atcgcgcgta
     5221 atgacctttg cgccatcgcg catcagctcg gaaaccgagc ggccatcgcg ggccccctcg
     5281 aggacaaagt cggtgatcaa cgcgacggcc tccggataat tgagcttcac tccgcgctct
     5341 aggcggcgcc gcgccaccat ggctgccatg gcgatcagca gcttgtcctt ttccctgggt
     5401 gtgagattca tcttgtgcgt ccccctccag atcagaattg ccaaagccgt ggaatggcag
     5461 ctcgcgttag tgcttgtaca gctcgatgaa gcacgcactg gacttccgac agcccattcg
     5521 cgagagcgcg catcacgagc atcccgcgcc aggcgctgac gccgtgcact cccggacaat
     5581 caaaaaggct tttccgcaca gattcgcgca tatcctcggc gttgggacca gtatgaatca
     5641 agagcgcagt ggcaatccca ttgtttgcaa tagccggacg tcccatcatc tcacctaaat
     5701 gaccttcgag ccgcacttcc tccgcaaaca gcaaacggcc atcacggcga atccgccatt
     5761 tatcccggaa cgctccgaac cccatttgct ccccgctcgc cgtgcgaccg aagatcgtcg
     5821 cttccccaat caataagctg gcattttcgg ccacatccgc ctcgatggag cgcgtcacgc
     5881 gagcgccgga gaaaagaatc gttggttgcg gcagcc
//
LOCUS       DASUMG010000272         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_276885, whole genome shotgun sequence.
ACCESSION   DASUMG010000272 DASUMG010000000
VERSION     DASUMG010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276885"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..437)
                     /locus_tag="VFX46_04530"
                     /pseudo
     CDS             complement(1..437)
                     /locus_tag="VFX46_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018454926.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-dependent oxidoreductase"
     gene            complement(457..840)
                     /locus_tag="VFX46_04535"
     CDS             complement(457..840)
                     /locus_tag="VFX46_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599882.1"
                     /translation="MGSWRAKSPFEDSPFIDSFDEWMDAPEGQDSIDALNLVFDALEH
                     AGVDARRRKIIWDDGKRLSIKQSVERIHAEHPDVPRGQIESHLCGWLENCAPESYSEQ
                     QLKELDQLVGPWINDYQRTSRTAKK"
     gene            975..2376
                     /locus_tag="VFX46_04540"
                     /pseudo
     CDS             975..2376
                     /locus_tag="VFX46_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611516.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2486..2803)
                     /locus_tag="VFX46_04545"
     CDS             complement(2486..2803)
                     /locus_tag="VFX46_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599883.1"
                     /translation="MKPGPKPSSGKAMTHAERQARYRTKRAEGGPTFRYRKPADRRSR
                     IQRWHDAVADLQALQNDYQAWLEALPENMADGATADALRAICDLDLSELQSIVPPKGF
                     GRD"
     gene            complement(2878..>3485)
                     /locus_tag="VFX46_04550"
     CDS             complement(2878..>3485)
                     /locus_tag="VFX46_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599884.1"
                     /translation="VRWKGGVLTDIDVPLPRSRPATIRTDEDTLDLVRRLATLYPDAV
                     IAGILNRQGRTTARGHRFEANRVATLRTHWRIPCFQRKPEIAEGDVMTIAKAAIVLNV
                     APSTLHRWLNDGLIAGEQVTPGAPWRIRMTDELKARFTASAGEGFITMQEATRALGVT
                     RQTVLQRVKRGEIEAAHLTKGGKVGLRIKLANRQPNLFEPA"
BASE COUNT          629 a         1136 c          990 g          730 t
ORIGIN      
        1 tcacggcgcc attggtggtc tggcctgaat ttccgctcgg cgatccagtt gatcaccggc
       61 tccttggagg aaatcggata ggcaacgaac ttttggaact cgtggccggc catgatcatg
      121 ctgcggccac tcaggaatgt ccgtgcccgc gtcacaccgc gccacaggat cgcaccattc
      181 cacagcggcg gtccctcgtc aggatacatc gcgctgcgta gagttgagtg aatgccatcc
      241 gcagcaatca gcagttcgcc cactgccgcg ccaatcgcac gaccactgcg acggttgatg
      301 aaagccgcgc gtacgccgtt ggctgtcgct tcccattcgg caaggtgatg tccagtggca
      361 atgcagtcag caccaagccg ctcatgcgct gcggccaaca ggatcatctg caattcgccg
      421 cggtgaatgg agaacttgag agtgcggagt tctcggctac tttttggcgg tgcgtgacgt
      481 gcgttgataa tcgttgatcc agggtccgac gagctggtcg agttccttga gctgttgttc
      541 ggagtaggat tccggcgcac agttttcgag ccagccgcag aggtgacttt cgatctgacc
      601 tcggggcacg tccggatgct cggcatggat gcgctccacc gactgcttga tcgacagccg
      661 cttcccgtcg tcccaaatga tcttgcgccg cctggcgtcg acgccggcat gttccagcgc
      721 gtcgaagacc agattgagcg cgtcgatcga gtcttgcccc tcgggcgcgt ccatccattc
      781 gtcaaagctg tcgatgaagg ggctgtcttc gaaggggctt ttcgcacgcc aactacccat
      841 tgcgtgagga tagcaccgtt ggtcgccaac gtcgcgggga tcacgccaga gtcaacgccg
      901 atcacgccag attcaacttt gttacagaga cgtcgggagt aacgacgtca tagcaaacat
      961 cagacgagtc cccgatgtcg ctgtggatgc actggtggaa cgcgatctgg cacctgcgtc
     1021 cggcgttctc ccgactgcag accttccttt ggttcgccac cgccgtggca ggcttcacgg
     1081 cgcgcaccga catgctgggc gtgacctcca tcgtgcgcgc gttgaacctc gatgctcgct
     1141 gctacaatgc gctgcgtgac tccttccaca gcgccgccgt caagctcgac cggctcaccg
     1201 cgctgtggac ccagctggcg ctacgcctgt tcccccagac cctgcgcgtc aatggcaggt
     1261 gcgttctggt cggcgatggc atcaaagtcg ccaagtgcgg tcgcaagatg cccggggtca
     1321 agctcctgca tcagcagtcc gaagccaaca ccaagccgga attcatcatg ggccactcac
     1381 tgcaggccgt gagcctgctc gtccatgccg cgtccacctg cttcgccgtg ccgctcgcca
     1441 tccgcatcca cgaaggattg gtatggtcca atcgcgaccg ccgcaccctg ctcgacaaga
     1501 tgctggccct cctcggtatc gccgccgtcg atcaaccgtt ctacttcgtc gccgatgcct
     1561 actacgccgc gcacaagatc gtcgccggcc tgctcgcgca gaacaatcac ctcctcaccc
     1621 gggtgaagtc caatgccgtc gcctacaccg cctatcagca gcgcggcccg cgcaagagag
     1681 gccggcccag ggtctatggc cgcaagatca ggctccactc cctgctgcag gaccgcaagg
     1741 ccttccagca ggcgaaaagc cccgtctacg gcgaggataa gctgatcatc cagtatcgcg
     1801 tccgcgacct gctctggcgt cccgcgggcc gcctggtccg cttcgtcgcc gtgatccatc
     1861 cggcgagagg caattgcatg ctgatgtgca ccgacacatc cctcgacgcg gtcgagatca
     1921 ttcgtctcta cggcctgcgc ttcaagatcg aacttacctt caagcaggcg gtccgccaga
     1981 tcggttcctt cgcctaccat ttctggatgc ccgacatgaa gccgatccgc cgccgcagtg
     2041 gtaaccagca tctccatcgc gcctcgtcca aataccgcga cgacgtcagg cggaaagatc
     2101 cacgcctacc acgtgttcat ccaggcgggc gtcgtctgcc agggactgct tcagtacctc
     2161 gccgtcgctt tccccgacct cgtctggaac tctttcggct cctggcttcg caccgttcga
     2221 cccggcatcc cgccctccga actcgtcgtc gccaccgcac tgcgccagcg cctgccagaa
     2281 tttctcttga atgccgtcaa aagctcgatc ttcatgaaat tcgtctccga tcgtcaggac
     2341 accagcaaaa tgcgcgcctt ccgccttgcc gcctgagtgt ctcggagcac aaactccaga
     2401 ctcacgagtt atgttttctc tgactgattt tagtggtcga tgatcaatcc aagtgcgagc
     2461 tcctcataga ttacatcgtg caacttcagt cgcgtccaaa acccttgggc ggcacgatgc
     2521 tctgcagttc agatagatca agatcacaga tcgcgcgcaa ggcatccgct gtagcgccgt
     2581 ccgccatgtt ttccggcaaa gcctcgagcc aagcctggta atcgttttga agcgcctgta
     2641 ggtcggcaac ggcatcgtgc catctctgaa tgcggcttcg acgatctgcg ggcttgcgat
     2701 accggaatgt gggcccgccc tctgctcgct tggttctgta gcgggcttgc ctttccgcat
     2761 gggtcatggc cttgcccgac gacggtttgg gtcctggttt cattgttcac gggttctcgc
     2821 gttggctccg ctgtaattcg tcacgtgacg aaatacagcg gcaggcgacg atcagcctca
     2881 ggcgggctca aaaaggttcg gttgccggtt ggcaagcttg atccgtaatc cgactttccc
     2941 acccttggta aggtgcgctg cttcgatctc gccacgcttt acacgttgca agacggtctg
     3001 acgggtgaca ccgagagcgc gggtagcctc ctgcatggtg ataaaccctt cccccgctga
     3061 ggcggtgaag cgtgctttta gctcgtccgt catgcggatc cgccagggcg cgcctggggt
     3121 gacttgctca ccggcgatca ggccgtcgtt caaccaacga tgcagggtcg atggcgcaac
     3181 gttcaagaca atcgccgcct tcgcgatcgt cataacgtcg ccttccgcga tctctggctt
     3241 tcgttggaag caggggatgc gccaatgggt ccgcaaggtc gcgacgcgat tggcctcgaa
     3301 ccggtggcca cgagccgttg ttcgaccctg tcgattgaga atgcccgcga tcacagcgtc
     3361 cgggtaaagc gtggcgagac ggcgcacaag gtccaacgtg tcctcgtcgg ttctgattgt
     3421 tgcgggtctc gatcggggca aaggcacgtc gatgtcggtc agcacgccgc ccttccagcg
     3481 taccg
//
LOCUS       DASUMG010000273         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_598011, whole genome shotgun sequence.
ACCESSION   DASUMG010000273 DASUMG010000000
VERSION     DASUMG010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598011"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1685)
                     /locus_tag="VFX46_04555"
     CDS             complement(120..1685)
                     /locus_tag="VFX46_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX5599885.1"
                     /translation="MRSAADLPDDVNELKRRLVAAEAGLLAKTLEAEKLKFELARLKR
                     MSFGQSSERIEREIEQLELKLEEIETTVAVAGPPEASEPDDPAPTASPEKKPRRKLPE
                     HLPRREERHEPGTCACPRCGCERLRKVGEDVTEVLEYVPGRFEVVRHVRPAYSCSKCE
                     AMTQASMPPMPIPRAMAGASVLAHVIMSKYADHLPLYRQAEIYARDGLDLDRSMLADW
                     MGKAAWLLRPLAERIGEHVMAGTVIHADDTPVPVLAPGNGKTKTGRLWIYLRDERPHA
                     GPAPPAVLYRYTPDRKGERCREHLAPFAGHLHADGYAGFAELYESAEGKPANVTEVAC
                     WAHVRRKFFDVHKANGSAIAREALERIGALFDIERTIAGKSADRRRTVRQSLAREKLD
                     ELAGWLDRQQKLLSGKSELAGAIRYARSRWDALTAYVDDGRLEISNNAAENAIRPVTL
                     GRKNWLFAGSDAGGDRAAIFYTLIRSAKLSGLEPEAWLRDVLTRIGHHSINRLDELLP
                     WIMAQPATHSVAA"
     gene            complement(1711..1968)
                     /gene="tnpB"
                     /locus_tag="VFX46_04560"
                     /pseudo
     CDS             complement(1711..1968)
                     /gene="tnpB"
                     /locus_tag="VFX46_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465831.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; incomplete; partial in the middle
                     of a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2026..2127)
                     /gene="tnpB"
                     /locus_tag="VFX46_04565"
                     /pseudo
     CDS             complement(2026..2127)
                     /gene="tnpB"
                     /locus_tag="VFX46_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          347 a          731 c          675 g          376 t
ORIGIN      
        1 cagagtctgc accaggtcct gcgtgctcct actccaacgg ctgagctcag tgacgaggat
       61 ggcgtcaatc gtaagcgtcc gttgcaagct gcggccgttt cggcttgacg gaggtgcgtt
      121 caggcggcga cgctgtgcgt cgccggttgc gccataatcc aaggcagcaa ttcgtcgagc
      181 cggttgatcg aatggtggcc gatgcgggtg agtacatccc gcaaccatgc ctcgggttcg
      241 agaccgctga gtttggctga gcgtatcagg gtgtagaaga tcgcggctct gtcaccgccg
      301 gcatcgctgc cagcgaagag ccagttcttg cgacccaagg tgacgggccg gatcgcattc
      361 tcggcggcgt tgttggagat ttccagccgc ccatcgtcca cataggccgt cagggcatcc
      421 cagcgtgatc gggcatagcg gatggcgccg gcgagttcgc tcttgccgga cagaagcttt
      481 tgctgccggt cgagccatcc ggcgagctcg tcgagcttct ccctggccaa gctctggcgc
      541 acggtccggc gtcggtctgc agacttgcct gcgatcgtgc gctcgatatc gaacagcgct
      601 ccgatcctct ccagcgcctc gcgtgcgatg gccgagccgt tggccttgtg cacgtcgaag
      661 aacttgcgcc gcacgtgagc ccagcacgca acctcggtga cgttggcagg cttgccctcg
      721 gcgctctcgt agagctcagc aaagccggca tagccgtcgg cgtgcaggtg gccagcgaat
      781 ggcgccaagt gttcacggca gcgctcgccc ttgcggtcgg gcgtgtagcg atagagcaca
      841 gcgggcggcg ccggtccggc atgcgggcgc tcgtcacgca gatagatcca cagccgaccg
      901 gtcttggtct tgccgttgcc gggcgccagc acgggcaccg gcgtatcgtc ggcatggatc
      961 accgtgcccg ccatcacgtg ctcaccgatc cgctcagcca gagggcgcag cagccacgcc
     1021 gccttgccca tccagtcggc caacatgctg cgatcaagat caaggccgtc gcgcgcgtag
     1081 atctcggcct ggcgatacaa gggcagatgg tcggcgtact tcgacatgat cacgtgcgcg
     1141 agcacgctgg caccggccat ggcacgcggg atcggcatcg gcggcatgga tgcctgtgtc
     1201 atggcctcgc acttggagca cgagtaggcc ggccgcacat ggcgcaccac ctcgaagcgg
     1261 ccgggcacgt actccaagac ctcggtcacg tcctcgccga ccttgcgcag gcgctcgcag
     1321 ccgcagcgcg ggcaagcgca ggtgccgggc tcgtgcctct cctcgcggcg tggcaaatgc
     1381 tcgggcagct tgcggcgcgg cttcttctcg ggcgatgcgg tcggcgcggg atcatcaggc
     1441 tccgaggctt cgggcgggcc cgcgaccgct actgtcgtct cgatctcctc aagcttcagc
     1501 tccagctgtt cgatctcgcg ctcgatgcgc tccgacgatt ggccgaagga catccgcttt
     1561 agccgcgcca gctcgaactt cagcttctcc gcttccagcg tcttggccag caatcccgct
     1621 tcggccgcaa ccaaccgccg cttcagctca ttgacgtcgt cgggcaggtc ggcggcgctg
     1681 cgcatgccga cgccatacgc tgcttcgccg ctatcccgcg actgtcggct gccacgtgcg
     1741 ttgaggagtt cgccaatcga tgccttccag caacatcgac agctgcgccg gcgtcagcgt
     1801 gacggaaccc tccttcgtga tcggccaaac gaagcgcccc ttctccagcc tcttggcgaa
     1861 caggcagaaa ccctgaccgt cccagtagag gaccttcacc agatcgccgc gcttgccgcg
     1921 gaacacgaac aggtggcccg agaacggatc cttcttaagt agctcctgcg ccagcatcga
     1981 cagctgcgcc ggcgtcagca ccgcaactcc ttgttcggtg cgcggccaca cgaaccggcc
     2041 ccgttcgagc cgcttggcga gcaggcacag cccgtcgccg tcccaccaca gcagcttgac
     2101 gaggtcgccg cgccgcccac ggaacacga
//
LOCUS       DASUMG010000274         2211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_518263, whole genome shotgun sequence.
ACCESSION   DASUMG010000274 DASUMG010000000
VERSION     DASUMG010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518263"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VFX46_04570"
     CDS             complement(<1..677)
                     /locus_tag="VFX46_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX5599886.1"
                     /translation="MPSNDVVIRSAILATFRDLAERRGLDCHGLLEEAGLPRNALDNP
                     ETELPLNSVASLMELAAERADDPSLGLTLAEAHHPGASGLVDYIALHASTVGESLQAI
                     VDYSGLICHPSPLQLRKKRDSLALSWSFPTVKGTRRVQFASFINAVIVLRLRHLAGRQ
                     WSPLAMEVEHPELPCPAMVRRIFGRRITFNARQNQILIDRKTAGQAIRGADPNLFRIL
                     REFGDVK"
     gene            885..1403
                     /locus_tag="VFX46_04575"
     CDS             885..1403
                     /locus_tag="VFX46_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5599887.1"
                     /translation="MSRTQDQSQLSSALHLLHRAGQRADELFTARMGEKDLTPRQYAV
                     LKAVASMENPSQTALVEYTGIDRSTIADIVRRLIKRGMLQRRRTAHDARMYAVKLTPS
                     GREALEAAEPIAKATEEQLLSVLPAQQRSIVVQALSTIVDKLKIEANGSKGAEGAAAS
                     ATGRRRRSSARQ"
     gene            1548..1997
                     /locus_tag="VFX46_04580"
     CDS             1548..1997
                     /locus_tag="VFX46_04580"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX5599888.1"
                     /translation="MTEVLFYHLERQPLERVLPTLLERTLERGWRAVVQCASEERLEA
                     IDGWLWTYREDSFLPHGTIRDGPPALQPIFLTTGEENPNGANVRFLVEGAVISDFKPY
                     DRVVLIFNGHDAAAIELARAEWKRARAMGCEVTYWQQADNGRWERRA"
BASE COUNT          450 a          633 c          689 g          439 t
ORIGIN      
        1 aatttgacat ctccgaactc ccgcaagatg cggaagaggt tcgggtccgc gcctcgaatt
       61 gcttggcccg cggtcttgcg gtcgatcaga atctggttct gccgggcatt gaacgtgatg
      121 cggcggccga aaatccggcg aaccatagcc gggcagggca gctccggatg ctccacttcc
      181 atggcgagcg gactccattg gcgaccggcc aaatgcctga gccgcagaac gataaccgca
      241 ttgatgaagc tcgcgaactg aacgcggcgc gtgcctttta ccgtggggaa agaccaggat
      301 agcgcaaggg aatcgcgctt cttgcgaagc tgcagcggac ttggatggca aatgagacct
      361 gagtagtcga cgatagcctg aagggactcg cctacggtgc ttgcatgcag tgcgatgtag
      421 tcgacgagac ccgaagcacc cggatggtgt gcctccgcaa gtgtcagccc gaggctaggg
      481 tcgtcggctc gctcagcggc gagctccatg agtgacgcca cggagttcag cggcagctcg
      541 gtttcaggat tgtccaaagc atttcttggc aaccccgcct cctcgagaag gccatggcag
      601 tcgaggccac gcctctcggc gaggtcgcgg aaagtcgcaa ggatggcgga gcgaataaca
      661 acatcattgg aaggcataac tgcagatcat tgtctacccg atggaaacgc ccaactgcgc
      721 caatcgccgc gattctttgc acctagcgtc aagaagcatg caccagaggt caagtctgca
      781 tgcccctctt cacgtaaatc ctctcgaaaa atcagaatca tgacaatggg ttgcggacgc
      841 ccagctcttg tcttgtcata tctttcctgg agcctttccc tacgatgtct cggacgcaag
      901 accaatccca gctgagttct gccctgcacc ttctgcatcg tgccggtcag cgtgcagatg
      961 agttgttcac ggccaggatg ggcgagaagg atctcacgcc acggcagtat gcggtgctca
     1021 aggcggtcgc gagcatggag aacccaagcc agacggcgct ggtcgagtac accgggattg
     1081 atcgctcgac tattgcggac atcgtgcggc ggctgatcaa gagaggcatg ctgcagcggc
     1141 gccggactgc tcatgacgcc agaatgtatg ccgtcaagct gacgccctcc ggccgggagg
     1201 ctctcgaagc cgctgagcct attgccaagg cgacggagga gcagctcctt tctgtgctgc
     1261 cggctcagca gcgctcgatt gttgttcagg cgttgagcac aatcgtcgat aagctcaaga
     1321 ttgaggcgaa cggcagcaag ggtgctgaag gtgctgctgc ctccgcgacc ggtcggcgta
     1381 gacgttcttc agcgcggcag tagcacacgt tccggttcgg ctgggcttct gatcgggatg
     1441 gtcggctcag ccgttctcga ctagcaatgg ttctccatcg cctctgcggc gtggcttcgt
     1501 ctgctatatg ctattcgtgc cgcgttcacg gggtggaagg ggaacaagtg accgaggtgc
     1561 tattctacca tctcgagcgc cagccgcttg agcgggttct gccgacactt ctcgagcgca
     1621 cgcttgagcg tggctggcgc gcagtggtcc agtgcgcgag cgaggagcgt ctggaggcga
     1681 tcgacggctg gctctggacc tatcgcgagg acagctttct accgcacggc acgatccgcg
     1741 acggcccgcc tgctttgcag cccatcttcc tgacgacggg tgaggagaac ccgaacgggg
     1801 cgaacgtccg tttcctggtc gaaggggcgg taatttcgga cttcaagcct tacgatcgtg
     1861 tcgttttgat cttcaacggc cacgatgcag ctgccatcga gcttgcccga gcggagtgga
     1921 agcgggcacg ggccatgggc tgcgaggtga cctactggca gcaggcggat aatgggcgct
     1981 gggaaagacg cgcctgattg ctctctcgcg ctcaagtgag agaggctgcc ttcgtcgata
     2041 tccaaaatcc accaggagtt gaaacaaacg ggccttcaag atccccggtg caattcttgc
     2101 ctctttagcc gacctccgaa tgctcggaag tttcgcgacg cgatgtgagt gccgcgattg
     2161 aaacatacgt cacaaacgcg agcgcaagtg cggcgatata cggcgtttca a
//
LOCUS       DASUMG010000275         3914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_277023, whole genome shotgun sequence.
ACCESSION   DASUMG010000275 DASUMG010000000
VERSION     DASUMG010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3914
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277023"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFX46_04585"
     CDS             <1..792
                     /locus_tag="VFX46_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599889.1"
                     /translation="RICGPEFALWVARHDRFHAPLITDPALPYVAAWAWFADHPETLG
                     GQLLRRPWTPNMSPRRAFDEVTAWRKRIRLALTLSALSRKPWLSEGSALGYEFVELRT
                     IEDFINESDAMDNCLDSFSEKLESGVSYVFSIRRNGSPVADIEIGAHPVDPTLPTIVQ
                     LRGPRNRRAHPEIWRAAYSWLGSQELHPVPPQVVNSAARRRAWRALWKPYLATLSPAD
                     REQFEKIALELDKLRTRRRTRTQRMRTATAQRPDPILSEDAGTAC"
     gene            complement(833..2422)
                     /locus_tag="VFX46_04590"
     CDS             complement(833..2422)
                     /locus_tag="VFX46_04590"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX5599890.1"
                     /translation="MTKADLFPLTSARTVHRNNHSVELIEQAIRRAEGRLASTGALVV
                     ETGVHTGRSAQDKFTVRDEQTEGTIWWDNNKAMSREHFDVLHRDFLEHIKGRDLFVQD
                     LYAGADPASRLNVRVVCEYAWHALFIRYLLRRPGLNELSNFEPGLIVVDLPSFRADPA
                     RHGCRTETVIAVDLSRRLVLIGGTSYAGEMKKSVFSFLNYVLPEQGIMPMHCSANVGP
                     AGDAAVFFGLSGTGKTTLSADPKRTLLGDDEHGWSENGIFNFEGGCYAKTIRLSAEAE
                     PQIWAASNRFGALLENVVLKPGTAEPDFDDGRLTENTRSAYPLEFISNASTTGMAGHP
                     KNIVMLAADAFGVLPPIAKLTPSQAEYHFLSGYTAKVAGTEKGLGSEPQATFSTCFGA
                     PFMPRHPSVYGNMLRELIAKHKVDCWLVNTGWTGGKYGVGHRMPIQVTRALLDAALSG
                     TLKGQPMRTDPIFGFQVPTSLPGVDSKILNPRETWSDPAAYDEQAKALVEMFNKNFAT
                     FESHVDADVRAAAPTLRQAAE"
     gene            3086..3793
                     /locus_tag="VFX46_04595"
     CDS             3086..3793
                     /locus_tag="VFX46_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5599891.1"
                     /translation="MKEKSTIALVDDERNILTSLRMALEAEGYKVRTYTDGASALEGL
                     TEEPADLGIFDIKMPRMDGMELLRRIRQQSDMPVIFLTSKDEEIDELFGLKMGADDYI
                     AKPFSQRLVVERVKAVLRRSQPRDGAGNQAETSRVIERGKLRLDPERHTCHWDGKPVT
                     LTVTEFLILQALAARPGVVKTRDALMDAAYDDQVYVDDRTIDSHIKRLRKKFKQVDEN
                     FDVIETLYGVGYRFKEV"
BASE COUNT          775 a         1213 c         1207 g          719 t
ORIGIN      
        1 cgcatctgcg gtcccgagtt cgcgctgtgg gtggcaaggc acgaccggtt ccatgcgccg
       61 ctcatcacag atccagcgct cccttacgtc gcggcctggg cctggtttgc ggatcaccct
      121 gagacactcg gcgggcagct gctgaggcgg ccatggacgc ccaacatgag ccctcggcgt
      181 gccttcgacg aggtcacggc gtggcgcaag cgcatccggc tcgctttgac attgagcgca
      241 ctttcccgaa agccgtggct cagcgaagga tcggcgctcg gctacgagtt cgtcgagctc
      301 cgcacgatcg aggatttcat caacgaaagc gacgcgatgg acaactgtct cgactccttc
      361 tcggaaaagc tggagtcggg cgtcagctac gtgttcagca tccggcgaaa cggctcgccg
      421 gtagccgaca tcgagatcgg agcgcaccct gtagacccta ccctgccaac catcgttcag
      481 cttagagggc cgaggaacag gcgggctcat ccagaaatct ggcgtgccgc ttacagctgg
      541 ctcggaagcc aggagctcca tcccgtgccg ccgcaggtgg tcaactcagc cgcccgccgg
      601 cgtgcctggc gcgccctgtg gaagccttac ctcgcgaccc tctcgcccgc tgatcgggag
      661 cagttcgaga aaatagctct ggagctcgac aagttgcgca cccgcaggcg gacccgcacg
      721 cagcggatgc gaacggccac ggcgcagagg ccggacccta ttcttagcga agatgccggc
      781 accgcctgtt gagcggcacc ggcatctgaa gacacccgac tgttgaaggc tcttattcgg
      841 ctgcctggcg cagcgtcggc gcagccgccc tcacgtcagc atcgacgtgg ctctcgaagg
      901 tggcgaagtt cttgttgaac atctcgacaa gtgccttcgc ctgctcgtcg taggccgccg
      961 ggtctgacca cgtctcgcgc gggttcagaa tcttgctgtc cacacccggc agcgatgtcg
     1021 gcacctggaa gccgaaaatc ggatcggtcc gcatcggctg gcccttgagc gtccccgaga
     1081 gagccgcatc gagcagtgcg cgggtcacct ggatcggcat tctatgccca acgccgtact
     1141 tgccgccggt ccagccggtg ttgacgagcc agcaatcgac cttgtgcttc gcgatcagct
     1201 cacggagcat attgccgtag acgctgggat ggcgcggcat gaacggggcg ccaaagcagg
     1261 tcgagaacgt cgcctgcggc tcggacccaa ggcccttctc cgtgccggcc accttcgccg
     1321 tatagcctga gagaaagtgg tattcggcct ggctgggagt gagcttagcg atcggcggca
     1381 acacgccgaa ggcatccgcc gccagcatca cgatgttctt gggatgcccg gccatgccgg
     1441 tggtgctggc attggagatg aactcgagcg gatatgcgga gcgggtgttc tcggtcagcc
     1501 ggccgtcatc gaagtctggc tcggcggtgc caggcttcag cacaacgttt tccagcagcg
     1561 caccaaagcg attcgaggcg gcccagatct ggggctcggc ctcagccgac aaccgaatgg
     1621 tcttggcgta gcagccgccc tcgaaattga agatgccgtt ctcggaccag ccgtgctcgt
     1681 cgtcaccaag gagcgtgcgt tttgggtccg ccgaaagcgt cgtttttcca gtgccggaca
     1741 ggccgaagaa gaccgcagcg tctccagccg gccccacgtt ggccgagcaa tgcatcggca
     1801 tgatgccctg ctcgggcagc acgtagttca ggaacgagaa aaccgacttc ttcatctcgc
     1861 cggcatagga agtgccgccg atcagcacca agcggcggct gagatcgact gcgatgaccg
     1921 tctcggtccg gcagccatgc cgcgcgggat cggcgcggaa gctgggcaga tcaacgacga
     1981 tcaagccggg ctcgaaattc gacagctcgt tcagcccagg ccgccgcagc aaataccgaa
     2041 tgaaaagcgc gtgccatgcg tactcgcaaa ccacgcgcac attgagccgg cttgctggat
     2101 cagcccctgc gtacagatcc tgcacgaaga gatcgcggcc tttgatgtgc tccaggaaat
     2161 cccgatggag cacgtcaaag tgctcgcggc tcatggcctt gttgttgtcc caccagatgg
     2221 tgccttccgt ctgctcatcg cggacggtga acttgtcctg ggcggagcga cctgtgtgta
     2281 cccctgtttc tacgaccagc gctccggtgc ttgccaaccg gccttcggcc cgcctgatcg
     2341 cctgctcaat cagctcgact gagtggttgt tgcggtgaac ggtacgcgcg ctcgtcaggg
     2401 gaaacaggtc tgccttcgtc attttccttc aaccgtccgt tggaggggca caagacacct
     2461 ccgacctacc tgatgctaga actaccgcag cttcagcttc gcgcgccctc gcggaaagtt
     2521 tgccgcagaa cccggcgcgc caagtcgcca gccgcgttac tttggccccg tcagatagga
     2581 cacaaactac gaaatccgtg aagggtcacc gtatacattg tggtccaatc agcaatcacc
     2641 ggccggcggg tgcgactgaa gtccagacaa aattgccatc aacaaagacc ggtagcaccc
     2701 ttactgtcgg caggtgggcc cttttgagtc ccacaaggga tcgctgcaag ccagctcgga
     2761 tgggtgcgct atcaggttcc gttgttaacg caacacgaga catctaatct ataagtgctc
     2821 gagagaactg cgggcgaaag acatcaacgc gcttaagcga aaccgagttc tgcgattgca
     2881 ggtgcgacga actgccgggg gatgccatct gttcaccttc ttacgcggtt caacccaaga
     2941 ttgttgttgg caaaacgtcg tctcggtagg cttgtcggcc tattcatcgt agggttaaca
     3001 atccgttggt aagtcggacg cgccacattt cgcacaagaa cgggtcatag agcccggtca
     3061 caatgaccgt ccagggcaag agaccatgaa agagaagagt acgattgctc tcgtagacga
     3121 cgagcggaac attctcacct cgctcaggat ggctcttgag gctgaggggt acaaggtacg
     3181 gacctacacc gacggcgcat ccgcgctcga agggctgacc gaggagcctg ccgacctcgg
     3241 catcttcgac atcaagatgc cgcgcatgga cggaatggag ctgctgcgcc ggatccgcca
     3301 gcagtcggac atgccggtga tcttcctcac ctccaaggat gaggagatcg acgagctctt
     3361 cggcctcaag atgggcgccg acgactacat agcgaagccg ttctcgcagc ggctcgtcgt
     3421 ggagcgcgtc aaggccgtgc tgcgccgctc gcaaccgagg gacggcgctg gcaatcaggc
     3481 cgaaacctcg cgggtgatcg aacgcggcaa gctgcgcctc gaccccgagc gtcatacgtg
     3541 ccactgggat ggcaagccgg tgacgctgac tgtcacggaa ttcctgatct tgcaggcgct
     3601 ggccgctcgc cccggtgtcg tgaagacccg cgatgccctc atggacgcgg cctacgacga
     3661 ccaggtttat gtcgacgacc ggacgatcga cagccacatc aagcgattgc gtaagaagtt
     3721 caagcaggtc gacgagaatt ttgacgtgat cgagacgctt tatggtgtag gctatcggtt
     3781 caaggaagtc tgagccccgg ggggttcatg gcgatcgagc tggagcggtc ccggcccgtc
     3841 gttgatctat ggcggaccgc aaaagagggg ttcctgcgcg tacaccgcgc ggcgagtccc
     3901 gtcaggcagt ggct
//
LOCUS       DASUMG010000276         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_277116, whole genome shotgun sequence.
ACCESSION   DASUMG010000276 DASUMG010000000
VERSION     DASUMG010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277116"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFX46_04600"
     CDS             complement(<1..236)
                     /locus_tag="VFX46_04600"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5599892.1"
                     /translation="MDARTFDKSKLPSRHVTEGPSRAPHRSYYYAMGLTEEQIKRPFV
                     GVVSCWNEAAPCNIALMRQAQSAKKGVDEAGGTPR"
     gene            complement(573..3230)
                     /locus_tag="VFX46_04605"
     CDS             complement(573..3230)
                     /locus_tag="VFX46_04605"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX5599893.1"
                     /translation="MLQKTYQPAEIEKRIYEEWEKAGAFRAGRPERVNAEPYCIVIPP
                     PNVTGSLHMGHALNNTLQDILCRFERMRGKDVLWQPGTDHAGIATQMVVERQLMEQQI
                     TRRDLGREKFVEKVWEWKEHSGGMIINQLKRLGASCDWSRERFTMDEGLSRAVLKVFV
                     ELYKRGLIYKDKRLVNWDPRFQSAISDLEVMQVEVKGHLWHLRYPLKDDPSRYIVVAT
                     TRPETMLGDTAVAVHPEDERYKELVGKTVVLPLVGREIPIIADEYTDPEKGSGAVKIT
                     PAHDFNDFEVGRRHNLPMINIFDAEARINENAPEAYRGLDRFEARKRVVADLEAQGLL
                     EKVEPYTHMVPHGDRSGVPIEPWLTDQWYVNAAELAKEAIKAVEDGRTKFVPKNWEKT
                     YFEWMRNIQPWCISRQLWWGHQIPAWYGPDGEIFVEETENLALAAAQRHYGTRVNLVR
                     DPDVLDTWFSSALWPFSTLGWPDKTPELERYYPTNVLVTGFDIIFFWVARMMMMGLYF
                     MKEVPFRDVYIHALVRDERGQKMSKSKGNVIDPLELIDTYGADALRFTLAAMAAQGRD
                     IKLSRGRVEGYRNFATKLWNAARFAEMNECVPLKDFDPSSVRLTVNRWIAGEAEKAVA
                     EIQQAIETYRFNDAAGAAYRFVWNVVCDWYRELVKPILGGTDNEAKSETRAMLAWTLD
                     QILKLLHPFMPFITEELWAHMVEHGVKRDTLLALSPWPQLDGLAHAEADKELGWVVSL
                     VSEIRSVRSEMNVPPGAKVPLVLVGASETIRRWADEHQETISRLARLASIEFEDVPPA
                     GAAVIAFGDTTAALPLAGIIDMDAEKARLRREIDKAAADLAKIDAKLANPQFLAKAPE
                     EVVEEQRERKAELEALSARLANALKRLEA"
     gene            complement(3383..3652)
                     /locus_tag="VFX46_04610"
     CDS             complement(3383..3652)
                     /locus_tag="VFX46_04610"
                     /inference="COORDINATES: protein motif:HMM:NF022155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HEX5599894.1"
                     /translation="MTTSATAAIQSGATQQAGVAEPAAAASPKTSGPAPQARSSGRTL
                     EETVVELLRPMLREWLDANMPHIVEKALRAELSSISKRPAADDTR"
     gene            3796..>4231
                     /locus_tag="VFX46_04615"
     CDS             3796..>4231
                     /locus_tag="VFX46_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599895.1"
                     /translation="MDRLEATAERAFPAAHRQALRYWAPAQESAEERVSVAPATGSPV
                     RLESLPKAIARRSGEHQTAISPRAAVGQALKRAAHPLLSPVQARLQGQRQAVRGGHSW
                     VHVQSQEADRSEAEPMAWCWRLDRKASLCAVLQPLARRVQEAP"
BASE COUNT          724 a         1338 c         1340 g          829 t
ORIGIN      
        1 cgcggcgtgc cgcctgcctc gtcgacaccc ttcttggccg actgggcctg ccgcatcaga
       61 gcaatgttac agggggctgc ctcattccag cacgagacga caccgacgaa gggccgcttg
      121 atctgctcct cggtgagccc catggcgtag tagtaagagc ggtggggcgc acgtgacggc
      181 ccttccgtga cgtgacggct cggcagcttc gacttatcaa acgttctggc gtccattcta
      241 acctgacctc ccacggagtg tggcaaattc gtgtgcctgc atatacacgc acgcccgcac
      301 tctgaggagg ccagcctttc gtctcagcaa tgttctacaa gaaacccgcc actcggcacg
      361 cggatgaata ctcttgagcg ggggcaacca cattcatttc gggttcatat acgtggtcgg
      421 cctttgtggc tcaaaagagg caataggggc ggatttcgtt gccacgatgc cacagtggtg
      481 aacggcaggt tagtgcttgt gaaaaggtga ggtggcgaac ggtgagctgg gcgataagcc
      541 gcccagctac tcactccgca attcgctctt cttcacgctt caaggcgctt cagcgcattg
      601 gcgagccgtg ctgacagcgc ttcgagctcc gccttgcgct cgcgttgctc ctccacgacc
      661 tcctcgggtg ccttcgcgag gaattggggg ttggcgagct ttgcatcgat cttggcgagg
      721 tctgctgccg ccttatcgat ctcccgccgc agtcgggctt tctcagcgtc catatcgatg
      781 atgccggcga gcggaagagc agcagtcgtg tcgccaaacg cgatgactgc cgcacctgcc
      841 ggtggcacat cctcgaactc gatggaagca agccgtgcca ggcgactgat cgtctcctgg
      901 tgctcgtcag cccaccgccg aatggtctcg ctcgcgccca cgaggacgag cggcaccttg
      961 gcgccaggcg gaacgttcat ctccgaccgg acggagcgga tctcgctgac gaggctgacg
     1021 acccacccga gctccttgtc agcttcggca tgggcgaggc catcgagctg cggccagggg
     1081 ctcagcgcaa gcagtgtgtc gcgcttgacg ccatgctcaa ccatatgcgc ccacagctcc
     1141 tccgtgatga agggcatgaa ggggtgcagc aacttcagga tctgatcgag cgtccaggcg
     1201 agcatggcgc gcgtttcgga cttggcctcg ttgtccgtgc cgccgaggat tggcttcacg
     1261 agctcacggt accagtcgca gacgacgttc cagacaaagc ggtaggcggc gccggcggcg
     1321 tcgttgaagc ggtaggtctc gatcgcctgc tggatctctg ccaccgcctt ctcggcctcg
     1381 ccggcgatcc agcggttgac ggtcaggcgc accgatgaag gatcgaagtc cttcaggggc
     1441 acgcactcgt tcatctccgc gaaccgcgca gcattccaga gcttggtcgc gaagttccga
     1501 tagccctcga cgcggccgcg ggaaagcttg atgtcgcggc cctgcgctgc catggccgcg
     1561 agcgtgaagc gcagcgcgtc ggcgccgtat gtgtcgatga gctccaaggg gtcgatgacg
     1621 ttccccttgg acttcgacat tttctgccct ctctcgtctc ggacaagagc gtggatgtag
     1681 acgtcgcgga acggcacctc tttcatgaag tagaggccca tcatcatcat cctggcgacc
     1741 cagaagaaga tgatgtcgaa gccggtcacg aggacgtttg tcggatagta ccgttcgagc
     1801 tctggtgtct tgtcgggcca gccgagcgtg gagaacggcc agagggccga tgagaaccag
     1861 gtgtcgagaa cgtccggatc acgaacgaga ttgacccgcg tgccgtagtg ccgctgcgca
     1921 gcagccagcg ccaggttctc cgtctcctcg acgaaaatct cgccgtccgg gccgtaccac
     1981 gccgggatct gatgccccca ccacagctgc cgcgagatgc accaaggctg gatgttccgc
     2041 atccactcga agtaggtctt ctcccagttc ttggggacga acttcgtacg gccatcctca
     2101 acggccttta tggcttcttt cgccagctcg gcggcattca cgtaccactg gtccgtgagc
     2161 cacggctcga tcggcacgcc cgaacgatcg ccgtgcggga ccatatgcgt gtaaggctcg
     2221 accttttcga ggagaccctg agcctccaga tcggcgacga cacgctttct cgcctcgaag
     2281 cgatcaagcc cgcggtaggc ctccggcgcg ttctcgttaa tccgcgcctc ggcatcgaag
     2341 atgttgatca tcggcaggtt gtggcgacgg ccgacttcga agtcgttgaa gtcgtgggcc
     2401 ggggtgatct tgaccgcgcc ggaccccttc tccggatcgg tatactcgtc cgcaatgatt
     2461 ggaatctcgc gcccgacaag cggcagcacg actgtcttgc cgacgagctc cttgtatcgc
     2521 tcgtcctccg gatgaacagc cacggccgta tcgccgagca tcgtctcggg ccgggttgta
     2581 gcgacgacaa tgtagcggga gggatcgtcc ttcagcggat aacgcaggtg ccagaggtgg
     2641 cccttcacct ccacctgcat cacctcgagg tcggaaatgg cggactgaaa ccgcgggtcc
     2701 caattgacga gccgcttgtc cttgtagatg aggccgcgct tgtaaagctc gacgaagacc
     2761 ttgagcaccg cccggctcag cccctcgtcc atcgtgaacc gctcgcgcga ccagtcgcac
     2821 gaggcgccca gccgcttgag ctggttgatg atcattccgc cggagtgctc tttccactcc
     2881 cagaccttct cgacgaactt ttcacgcccg aggtcgcgcc gcgtaatctg ctgctccatg
     2941 agctggcgct cgacgaccat ctgcgtggca atgccggcgt ggtccgtgcc cggttgccag
     3001 agcacatcct tgccgcgcat gcgctcgaac cggcagagga tatcctgcag cgtgttgttg
     3061 agggcgtgcc ccatatggag cgagcccgtg acgttgggcg gcgggatgac gatgcaatag
     3121 ggctcggcgt ttacccgctc cggccggccg gcgcggaagg cgcctgcctt ctcccattcc
     3181 tcgtagatcc gcttctcgat ttcggcaggt tggtaggtct tctggagcat gggtcgcttg
     3241 gcccggattc caaactaaac ggaagtgcag ggcgtcctac gccccgcatt cctcacctgt
     3301 caacacaagc gagcttgctg aggtccgcgg aaggtatcgc gcctcatcaa cggacccctc
     3361 tcggagggat ccccggcgca gttcagcgcg tgtcgtcagc agctggccgc ttcgagatag
     3421 aggagagctc cgcacgaagc gccttctcga cgatatgcgg catgttggcg tcgagccatt
     3481 cgcgcagcat cgggcgaagc agctcgacaa cagtttcctc gagcgtgcgc ccggatgatc
     3541 gcgcttgggg agcaggcccg ctcgtcttcg gcgaggctgc cgcagcaggc tccgccacgc
     3601 cagcttgttg agtcgctcct gattggatgg cagccgtcgc ggacgtggtc aaaggtttgc
     3661 tctcttgctc cgttcgctcc gggcgcgttg ccgtctgatc cagcgcgctg gcctcgcgcc
     3721 gctgaatgcc tgaaacaggt ccaggctgag gtgctggctc aggagcagcc ttggcttgga
     3781 tcggcgcagc agattgtgga ccgacttgag gcgacggctg agcgggcgtt tcccgctgct
     3841 caccggcagg cactccgcta ctgggctccc gcgcaggaaa gcgctgagga gcgggtctcg
     3901 gtagctcctg cgactggttc gccggtgcgg ttggaaagct tgccaaaggc gattgctcgc
     3961 cgttcgggcg agcaccaaac agcgatctcg cctcgtgcgg ctgttggaca ggctttgaaa
     4021 cgggcggctc accctctgct ttcgccggtg caggcgcggc tgcagggcca gcgtcaagct
     4081 gttcgcgggg gccactcgtg ggtgcacgtc caaagccagg aagcggaccg gtctgaggcg
     4141 gagccgatgg cgtggtgctg gaggctcgac cgaaaagcca gcttgtgcgc cgtccttcag
     4201 ccgctggcgc ggcgggtgca ggaggctcct c
//
LOCUS       DASUMG010000277         2047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_116925, whole genome shotgun sequence.
ACCESSION   DASUMG010000277 DASUMG010000000
VERSION     DASUMG010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116925"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFX46_04620"
     CDS             <1..438
                     /locus_tag="VFX46_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5599896.1"
                     /translation="LQKDCTTAVADIGKAVGLSTTPCWRRIQKLEEAGYIQKRVALLD
                     PKKVGAGVTVFVFISTSQHTLEWLERFHAVIVDFPEVVEFYRMSGEIDYLLRVVVPDI
                     DAYDRFYKKLISKIELSNVSSAFAMEQIKFTTALPLDYAPVEQ"
     gene            617..>2047
                     /locus_tag="VFX46_04625"
     CDS             617..>2047
                     /locus_tag="VFX46_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5599897.1"
                     /translation="MPHGEPKIALSTPVGDTVKTTTCYMCACRCGIRVYLKNGKVRYI
                     EGNRDHPVNKGVLCGKGSAGIMQHYSPARLRAPLKRVGPRGSGEFEEITWDEAMDLAT
                     KWLAEVREKDPSRLAFFTGRDQSQALTGWFAQQYGTPNYAAHGGFCSVNMAAAGLYTF
                     GGSFWEFGEPDWERTKYFMLFGVAEDHASNPIKTALGKLKARGVKIVAINPVRTGYNA
                     IADEWVGIKPGTDGMLIGALIHELLRTRNIDLEYLVRYTNAPWLVIRDPGAADDGLFA
                     RNESGKPLAFDKKTGALADATLPEIAPALVGEYKLPDGRTAVPSFALIAERFLSAEYS
                     PEKAAAITGVPAHTIRRLAQELAQAAFQQAISLNVPWTDWAGRRHETMIGRPVSMHAM
                     RGISAHSNGFQTCRLLHVLQILLGSIDCPGGFRYRPPFPKPTPPPNRPGGKLGTVIPG
                     KPLNGAPLGFPQGPEDLLVDDQGRALR"
BASE COUNT          389 a          628 c          647 g          383 t
ORIGIN      
        1 ctgcagaagg attgcaccac ggccgtagcc gacatcggca aggcggtcgg tctgtcgacc
       61 acaccctgct ggcggcgcat tcagaagctg gaggaggccg gttacatcca gaagcgcgtg
      121 gcgctgctcg accccaagaa ggttggagca ggggtcaccg tcttcgtctt catcagcacc
      181 agccagcaca cgctcgagtg gcttgagcgt ttccacgctg tcatcgtcga ttttcctgag
      241 gtggtcgagt tctaccgcat gagcggcgag atcgactatc tgctgcgggt cgtggtgccc
      301 gacatcgatg cttatgatcg cttctataag aagctcatct ccaagatcga gctgagcaac
      361 gtctcctccg ccttcgcgat ggagcagatc aagtttacga cggcgcttcc cttggattac
      421 gcgcccgtcg agcagtgagg attgcgagcg gtccgttctg agctactttt cggttagggc
      481 gctctggtcc aacggcggat attcgcgttt gtgaatgctt gttggacctg ccagcgtgtg
      541 ctagcagtct caaatctcga aacggatcgc atcgcccagc gatcctcagt caaagaacga
      601 tggggaaacg ccgtccatgc cgcacgggga accgaagatc gctctctcga cgcccgtcgg
      661 cgacactgtc aagacgacca cctgctacat gtgcgcctgc cggtgcggca tcagggtcta
      721 cctgaagaac ggcaaggttc ggtacatcga gggcaaccgc gatcacccgg tgaacaaggg
      781 ggtgctgtgc ggaaagggct ctgccggcat catgcagcac tactcgccgg cacggctgag
      841 agcacccttg aagcgcgtgg gtccgcgcgg ttccggcgag ttcgaggaga tcacctggga
      901 cgaagcgatg gatctggcca ccaaatggtt ggccgaggtc cgcgagaaag atcccagccg
      961 tctcgccttc ttcacgggcc gcgaccagtc ccaggcattg acgggctggt tcgctcaaca
     1021 atacggcact ccaaactacg ccgcccacgg agggttctgc tcggtcaaca tggctgcggc
     1081 ggggctctac acgttcggcg gctcgttctg ggagttcggt gagcccgatt gggagcgcac
     1141 gaagtatttc atgcttttcg gcgtggccga ggaccacgcc tcgaacccca ttaagacggc
     1201 cctcggaaag ctgaaggccc gaggcgtcaa gatcgtcgcc atcaacccgg tgcggacggg
     1261 ctacaacgcc atcgcggacg agtgggtggg catcaagccg ggaacggatg gaatgctcat
     1321 tggagcgctc atccacgagc tgctgcggac ccggaacatc gatctggagt atctggtccg
     1381 ctacacgaac gccccttggc tcgtgatccg cgatccgggt gcagccgacg atggcctctt
     1441 cgcgcggaac gagagcggca agccgctggc gttcgataag aagactggtg ccctagcaga
     1501 tgcaactctg ccagagatcg cgccggcttt ggttggcgaa tacaagttgc cggacgggcg
     1561 cacggctgtg ccgtcattcg ctctcatcgc ggagcgcttc ctgtcagctg aatatagccc
     1621 ggagaaggcg gcagcaatca ccggcgttcc ggcacacacg atccgccgtc ttgcccagga
     1681 gctcgcccag gctgccttcc agcaggcgat cagcctgaac gtgccgtgga ccgattgggc
     1741 agggcgccgg cacgagacga tgatcgggcg gccagtctcg atgcacgcga tgcgcggcat
     1801 ctcggcccac tcgaatggct tccagacctg ccgcttgctg cacgtgcttc agattctcct
     1861 gggctccatc gattgccctg gagggttccg ctacaggccg ccgttcccga aaccgacgcc
     1921 gccgcccaac agacccggcg gaaagctagg tactgtcatt ccagggaagc cgctcaatgg
     1981 cgcgccgctt ggatttccgc agggcccgga ggatttgctt gtcgatgatc agggcagggc
     2041 gctgcgg
//
LOCUS       DASUMG010000278         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_357757, whole genome shotgun sequence.
ACCESSION   DASUMG010000278 DASUMG010000000
VERSION     DASUMG010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357757"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2649)
                     /locus_tag="VFX46_04630"
     CDS             complement(<1..>2649)
                     /locus_tag="VFX46_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX5599898.1"
                     /translation="GWYSEEGTDSPEALEIALDKPNYKVGDVARLKIEDRYGGRAFVV
                     VLGDRLLASQEVELPKGGGELTIPVERDWGPGAYVTAMLYRPMDEQAKRMPSRAIGVA
                     WLPLDQSARTLNVSLEAQPQVRSGTDLTVPVKLEGLTAGEEARVTVAAVDVGILNLTR
                     YEAPAPERWFLGQRRLGTEIRDYYGRLIDGMRAERGRLRSGGDAEAGMGMQGSPPDGQ
                     TVALFSGIVRVGPDGAAQVHFDLPDFNGTVRLMAVAWTRDKVGHGSADVVVRDPVAVT
                     ASAPRFLTLGDQARLEVDLHNVEGVTGPFSLTVEQEVADGSLTTLAERELVLAANERK
                     SESFTIAPEHVGQTTYRIAIAGPGGLEIRRSLTLDVKPPAGDIRRVTISKLAPGASLT
                     LGADLLSDFIPGLTRVSVSVGQAAGLDVPGLLTQLDRYPYGCAEQVTSRALPLLYANQ
                     LAVTAGLPPDNELRQRIEDAIARVFEMQDSSGAFGTWGPSGGDMWLTSYVTEFLTRAR
                     EQGFSVKPQAFELALDRLQNFVNYAQDFEHGGEDRAYALYVLARNGRAPIGEVRYYAD
                     TRLDRFATPLAKAQLAAALAMLGDRARAEDVFRQALASADAADRNDRRDFGSVLRDRA
                     ALVTLASETRTAWNDAQPLVATLSQTYASRSHTSTQEQAWLLLAARALMDQSRDARLS
                     VNGVAHIGVLNRTFGADEIRAGDVVVRNEGQTAVDVMVSVNGAAMTPEPPIARNFKID
                     RAFYTLDGTPIELGSATGGGGQVRQTDRFVVVLKVEADVRGRILLVDRLPAGLEIENP
                     RLIDSADLRNFPWLRTSVQPEHTEFRDDRFVAAFDLGADEGQQSGSGRPVTFTVAYMA
                     RAVSPGSYVHPAATVEDMYRPERFART"
BASE COUNT          485 a          908 c          799 g          457 t
ORIGIN      
        1 cgtccgcgca aagcgctcag gccggtacat atcctcaacc gttgccgcgg ggtgcacgta
       61 gctgcccggc gaaacggcac gcgccatata agcgaccgtg aacgttaccg gccgtccgct
      121 cccgctctgc tggccctcgt cagctcccag atcaaacgcc gcaacgaacc gatcgtcccg
      181 gaactccgtg tgctcgggct gcacggatgt cctgagccac gggaaattcc ggagatccgc
      241 actgtcaatg agacgcggat tctcgatttc caatccggcc gggagacgat cgacgagcag
      301 aatccgtcca cgaacatccg cctcgacctt caaaacaacg acgaaacgat cggtttgccg
      361 aacctgtcca ccaccacctg ttgcactgcc cagttcgatc ggagtgccgt cgagggtgta
      421 gaacgcgcgg tcgatcttga agtttcgggc gatcggcggc tccggcgtca tggcggctcc
      481 gttcacagac accatgacat cgacagccgt ctgcccttcg ttgcggacga cgacgtcccc
      541 cgcgcggatt tcatccgcac caaaggttcg attgaggacg ccaatgtgcg ccacgccgtt
      601 gaccgagagc cgggcatcgc ggctctgatc catcagcgcc cgggcggcga gcagcagcca
      661 cgcctgctcc tgagtggatg tgtgggatct ggaggcatac gtctggctaa gcgtggcgac
      721 cagaggctga gcgtcattcc acgccgtacg cgtttctgag gctagcgtga ccagcgcagc
      781 tcggtcgcga agcaccgagc caaagtcccg ccgatcgttg cgatcggctg cgtcagccga
      841 ggcgagagcc tgccggaaga cgtcttccgc acgcgcccga tctcctagca tcgccaatgc
      901 tgcagcgagc tgcgccttgg cgagcggcgt ggcgaaacgg tcaaggcgcg tatccgcgta
      961 atagcggacc tcgccaatcg gcgcccggcc gttccgggcc agaacgtaga gcgcataggc
     1021 ccggtcctcg ccgccgtgct cgaaatcctg cgcgtagttg acgaagttct gcagccggtc
     1081 gagcgccagc tcgaacgctt gcggcttcac ggagaagccc tgctcgcggg cgcgcgtcag
     1141 gaactccgtg acgtagctcg tcagccacat gtcgccacca ctcggccccc aagtgccgaa
     1201 tgcgcccgag gaatcttgca tctcgaagac gcgtgcgatc gcatcttcga tccgttgtct
     1261 gagctcgttg tcaggtggca gcccagccgt gaccgcgagc tgattggcat agagcaacgg
     1321 cagcgcgcgg ctggtcacct gctcggcaca accgtacgga tagcgatcga gttgcgtcag
     1381 cagcccagga acatccaacc cagccgcctg cccaacgctg acagacaccc gggtcaggcc
     1441 ggggatgaag tcggatagga gatcagctcc gagcgtgagg ctggcaccgg gcgccagctt
     1501 gctgatggtc actcgacgga tgtcgccggc tggcggcttc acatcgagcg tcaggctgcg
     1561 tcgtatttcg agcccgcctg gcccggcaat cgcgatcctg tacgtggtct gaccgacgtg
     1621 ttccggcgcg atggtgaaag actcgctctt gcgctcgttt gcggccagca ccagctcgcg
     1681 ctcggccagt gtcgttaggc ttccatccgc gacttcctgc tcaaccgtga gcgagaaagg
     1741 tccggtgacg ccttcaacat tatgcagatc gacctcgagc cgcgcctgat cacccagcgt
     1801 caagaagcgt ggagctgagg cggtgacagc caccggatcg cgcaccacaa catctgctga
     1861 tccgtgcccg accttgtcgc gcgtccaggc cacagccatc aggcgaacag tgccgttgaa
     1921 atccggcagg tcgaaatgga cctgagcagc accatcgggt ccaacacgga cgatgccgga
     1981 gaacagagcg accgtttgcc cgtcaggcgg gctgccctgc atccccatac cggcttccgc
     2041 atctccgccg gaacgcaacc ggccacgctc tgcgcgcatg ccgtcgataa gccgcccata
     2101 gtaatcgcgg atctccgtac cgagacggcg ctgtcctagg aaccagcgct ccggggccgg
     2161 tgcctcgtac cgcgtcaggt taaggatgcc gacgtccacg gcggcgactg tgacacgcgc
     2221 ctcttctccc gcagtcagcc cctcaagctt gaccggtacg gtaaggtccg tcccggaccg
     2281 cacctgcggc tgagcttcca aggaaacgtt cagcgttcgt gcgctctggt ccagcggcag
     2341 ccatgcgacg ccgatcgccc ggcttggcat ccgcttggcc tgctcatcca tcggcctata
     2401 aagcatggcc gtgacataag cgccgggccc ccagtcacgc tcgaccggga tcgtcagttc
     2461 gccgcctcct ttcggcagct cgacctcctg gctcgccagc aggcgatcac ccaggaccac
     2521 aacgaacgcg cggccgccgt agcgatcctc gatcttgagc cgggcaacgt cgcccacctt
     2581 gtagttcggc ttgtcgagag cgatctcgag agcctctgga ctgtccgtcc cttcctcgga
     2641 gtaccatcc
//
LOCUS       DASUMG010000279         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_518810, whole genome shotgun sequence.
ACCESSION   DASUMG010000279 DASUMG010000000
VERSION     DASUMG010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518810"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1705)
                     /locus_tag="VFX46_04635"
     CDS             complement(176..1705)
                     /locus_tag="VFX46_04635"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HEX5599899.1"
                     /translation="MAIEGNLYAAFEERFRAAGSTIAFSIPGGADVTYSQLLDDVARM
                     ANALTALGVKPGDRVLVQVEKSISNVHLYLATLKIGAVYNPLNTAYTAAELDYFIRDA
                     EPALIVVPGEKLPHVEPIAAELGTAKIATMEADGSGSLTDLARGQSTEHRTVERDSDD
                     LAALLYTSGTTGRSKGAMITHRNLSSNAEVLHEYWGFEPGDVLLHALPIFHTHGLFVA
                     LNTTFLNTSRVIWLPKFDLEAVMRHMPEATVMMGVPTFYTRLLGNPAFGREHCARMRL
                     IISGSAPLLAETHKEFEARTGHKILERYGMTEAGMITSNPYRGGERIAGTVGYALPGI
                     SVRVASQDGKILPPNEVGVLEVKGPNVFKGYWRNPEKTKEEFRPDGYFITGDLATMAE
                     DGRVTIVGRAKDLIISGGFNVYPKEIEEELNALPGVNESAVVGVPHPDFGEGVIAILT
                     PEPGAKVPSEAEVISALSNRLAKFKLPKRVFVLDELPRNAMGKVQKGELRAQFAKTFT
                     G"
     gene            complement(1835..>2884)
                     /locus_tag="VFX46_04640"
     CDS             complement(1835..>2884)
                     /locus_tag="VFX46_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5599900.1"
                     /translation="ITPLLHAILTEPISWSALRALNGLCFAGLFMSIESWLSGASTIE
                     TRGRVLASYTVLNLTVVTLGMQLMAVGSPSDFQLFSLVAILYSLAAVPVALTRTNAPV
                     PSRTAKLRLAWLIGVSPAAVAGCFFNGVVNGAFWALAPLYGRSAGFSVAEVAMFMALA
                     VLGGAASLWPIGYLSDRVGRRPLLVILGATGAIAGIGLYVLSTSSPIAIMPLAVLWGC
                     SAFPIYTLCVAHANDLVHKKRAIEVSSGLLLTFSIGAVLGPLVAGYIMSVAGHGALFL
                     HTAAAHLLITLIMVVRARLRPTLPVEHKEVFVAVPKTTPLVFELDPRAEGAPPSASDV
                     VPQPSTQDFAAEQRA"
BASE COUNT          595 a          860 c          888 g          541 t
ORIGIN      
        1 ggcgtctcga aaaaacgcca tcccttcctg aaaaagatgg ccaagggtca agctattccc
       61 ttggccaatt ttgttgagcg ttcgttcgag agccgcagat atcgcggctt tccatcagtc
      121 agtttctcaa aggcagggcg agcttgattt tttcagcccg accgaaaaag ctccgctatc
      181 ccgtaaacgt ctttgcgaat tgggctcgga gctcgccttt ttgcaccttc cccatcgcat
      241 tgcgcggcag ctcgtctagc acgaaaaccc gtttcggcag cttgaacttg gctaaccgat
      301 tggagagggc agagatgact tcggcttcgc tgggaacctt ggctccgggt tcaggtgtca
      361 ggatagcgat cacgccttcc ccaaaatccg gatgaggaac gccgaccacg gccgactcgt
      421 taacgcccgg cagagcgttc agctcttcct cgatctcttt gggatagacg ttgaacccgc
      481 cggaaatgat gagatctttc gcgcggccca caatcgttac tcggccatcc tctgccatgg
      541 tggcaaggtc gccggtaatg aaatagccgt cgggccgaaa ctcctccttc gtcttctcgg
      601 gattgcgcca gtaccctttg aagacgtttg gccctttgac ctccagaacg ccgacctcgt
      661 ttggaggaag aatcttgccg tcctgagagg cgacccgcac tgagatcccg ggcagagcat
      721 aacccaccgt gccagcaatc ctctcgccgc ccctgtacgg gttcgaggtg atcatgccgg
      781 cttcggtcat accgtaccgc tcgaggatct tgtgcccggt gcgggcctcg aactccttgt
      841 gagtttcagc aaggagcggc gccgagccgg agataatcag ccgcatgcga gcgcagtgct
      901 ccctgccgaa cgccgggtta ccgagaagtc gtgtgtagaa ggtcggcacg cccatcatca
      961 ccgtggcttc cggcatgtgc cgcatgacgg cttcaagatc gaattttgga agccagatca
     1021 ctcgcgaggt gttgagaaac gtcgtattga gcgcgacgaa cagcccgtgc gtgtggaaga
     1081 tggggagcgc atggagcaac acgtcaccgg gctcgaagcc ccagtactcg tgcaggacct
     1141 ctgcattcga ggacaaattg cggtgggtga tcatggcgcc cttggagcgc cccgtcgtcc
     1201 cagaggtgta aagcagggct gccaggtcgt ccgaatcccg ctcgacggtt cgatgctctg
     1261 tgctctggcc gcgggctaga tcggtcagcg atccggaacc gtccgcctcc attgtcgcga
     1321 tcttggcagt gcctagctca gcagcaatcg gctccacgtg cggaagcttc tcgcctggca
     1381 cgacgatgag ggctggctcg gcgtctctga tgaaatagtc aagctctgca gcggtatagg
     1441 ctgtgttgag cgggttatag actgcgccaa tcttcagcgt agcaagatac aggtggacgt
     1501 tagaaatcga tttttcgacc tgaaccagaa cgcgatcgcc cggcttgacg ccaagtgctg
     1561 tcagcgcgtt cgccatgcgg gcaacgtcat cgagcagctg ggaataggtc acgtcggcgc
     1621 cacccggtat cgagaacgct atagttgagc cggcggcgcg gaaccgctct tcgaatgccg
     1681 cgtagagatt tccttcgatc gccatgagct ttgccgtctt ggttcagaac ttccagatca
     1741 ccagccgagg gcaggcggat aaaacgcttt ttggccgcga taggcaaggc cagggcgtca
     1801 attagaagtc gcgtcgatcc cccggcggac gggatcacgc tcgctgctcg gcggcaaagt
     1861 cttgcgtcga tggctggggt acgacgtcgc ttgcacttgg gggcgcacct tccgcacgcg
     1921 gatcgagctc gaagacgagg ggggtggtct tgggcactgc gacaaagact tccttgtgct
     1981 ccaccggcaa tgtcggccgc agtctggccc ggaccaccat gatgagcgtg atcagcaggt
     2041 gcgctgctgc cgtgtgcagg aagagcgcgc catgacccgc tacgctcatg atgtagccgg
     2101 caacgagcgg accgagcacg gcgccgatcg aaaaggtgag cagcaagccg cttgaaacct
     2161 cgatggcgcg cttcttgtgc accagatcgt tcgcgtgtgc cacgcagagg gtgtagatcg
     2221 ggaaggcgct gcagccccag agcaccgcaa gtggcatgat ggcgatcggg ctgctggtgc
     2281 tgagcacgta aagcccaatc cctgctatcg ccccggtggc gccgaggatc accaacaacg
     2341 gccgccggcc gacccggtca gacaagtagc cgatcggcca gaggctggca gctccgccga
     2401 gaaccgccaa cgccatgaac atcgcaacct cggcgacaga aaagcctgcg ctgcgcccgt
     2461 aaagcggagc gagcgcccag aatgcgccgt tgacgactcc gttgaagaag cagccagcaa
     2521 cagccgccgg cgagacgccg atgagccagg ccagccgcaa cttggcggtc cgcgagggaa
     2581 cgggtgcgtt tgttctggtg agtgcaacag gtacggcggc cagcgaatag agaattgcga
     2641 ccagcgagaa gagctggaaa tcgctggggc ttccaaccgc catcaactgc atgcccagcg
     2701 tcaccaccgt caggttcagg accgtgtacg acgcaagcac gcggccccgc gtttcgatgg
     2761 tcgatgcacc gctcaaccaa ctctcgatgc tcatgaagag gccagcgaag cagaggccat
     2821 tgagggcccg gagagcggac cacgaaatag gctccgtcag gatagcgtgc agcaacggcg
     2881 tgat
//
LOCUS       DASUMG010000280         2927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1161251, whole genome shotgun sequence.
ACCESSION   DASUMG010000280 DASUMG010000000
VERSION     DASUMG010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161251"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VFX46_04645"
     CDS             <1..609
                     /locus_tag="VFX46_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX5599901.1"
                     /translation="VEKAAQTIDAEKAQKIAQKMRKGEFDLEDLAEQIKQMQKIGGMS
                     GVLGMLPGIGKIRNQIAEANLDDKVLKRQHAIILSMTPKERRNPKIIDGKRKRRIAAG
                     SGTKVEEVNRLLKMHRQMADVMKAMGKNKGMLARMFGGGAMPQPTPEMVEQLQRGELP
                     KGLPGLPSGGVPRLPGLGGGLPGLGGAAPKFPGLPGFPGKKK"
     gene            880..1233
                     /gene="rpsP"
                     /locus_tag="VFX46_04650"
     CDS             880..1233
                     /gene="rpsP"
                     /locus_tag="VFX46_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX5599902.1"
                     /translation="MSVKIRLARGGAKKRPYYYIVVANSSAPRDGRYIEQVGAYNPML
                     KKDDPNRVKLDIDRCKHWLSVGAQPTDRVARFFDAAGLIKRQPKHNPIKGKPGKKRLE
                     REAAEAEKAKEATGA"
     gene            1265..1798
                     /gene="rimM"
                     /locus_tag="VFX46_04655"
     CDS             1265..1798
                     /gene="rimM"
                     /locus_tag="VFX46_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643927.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX5599903.1"
                     /translation="MGAAPARVLLGRIRAAHGIRGHVLIHTFTEAPEDIASYGPLQDE
                     SGERQFEITVVRVTPKGVVARISGVDDRTSAEALRGTQLYVERDRLPPTEEDEYYHSD
                     LIGLTAFKPNGERMGEVIAVHNFGAGDILEIRLKGKRTTEMVPFMRDFVPEVDVSAGR
                     LTVHMPGENEADRDQKS"
     gene            complement(1800..2507)
                     /locus_tag="VFX46_04660"
     CDS             complement(1800..2507)
                     /locus_tag="VFX46_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5599904.1"
                     /translation="MRAVVRVLDGETLALDDGSQVRLIGALAPRALDTGADEGTWPLA
                     EAAKSALEELSLGRSVELGFAGRRTDRYGRLMAQVFVRTDDKRLWLQGEMLRQGHARA
                     YALPGSSDCLDDLVAAEKQAREARLGLWEHASYQIRPANRHRELLRFRSTYQLVEGEV
                     LATADVRGRIYLNFGEDWRQDFTITVQPSNRRQFAEAGMDLLALKSRRVLVRGWIERR
                     GGPMIEVYHPAQLEILE"
     gene            2775..2851
                     /locus_tag="VFX46_04665"
     tRNA            2775..2851
                     /locus_tag="VFX46_04665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Arg,seq:cct)
BASE COUNT          592 a          927 c          898 g          510 t
ORIGIN      
        1 gtcgaaaagg ccgcccagac gatcgacgcc gaaaaggcgc agaagatcgc ccagaaaatg
       61 aggaagggcg agttcgacct ggaggacctc gccgagcaaa tcaagcaaat gcagaagatc
      121 ggcggaatgt ccggcgtgct cggaatgctg cccggcatcg gcaagattcg taatcagatc
      181 gccgaagcca atctcgacga caaggtgctg aagcggcagc acgcgatcat cctgtcgatg
      241 acgccgaagg agcgccgcaa cccgaagatc atcgatggca agcgtaagcg gcgtatcgca
      301 gccggctccg gaaccaaagt cgaagaggtc aaccgcctgc tcaagatgca ccgccagatg
      361 gccgacgtga tgaaggccat gggcaagaat aaaggcatgc tggcgcgcat gttcggtggt
      421 ggtgccatgc ctcagccgac ccctgagatg gtcgagcagc tgcagcgcgg cgagttgccg
      481 aagggcttgc cgggcctgcc gtccggtggg gttccgcgcc tacccggtct cggcggcggc
      541 ctgccggggc tgggcggagc agcccctaag tttccaggac ttcccggttt tccggggaag
      601 aagaagtagg ttcgggtcgc gtcgctatga tctgttgcaa cccgagacct tctcgcgagc
      661 tgtcatcgcg gcgcttagcg ccgggatccg ccttgcctct cgctcccgag cgaggcgatg
      721 gctggatcct gaacacaaga cccgaggtga cgtcgtgttc cgaccggcca atggctgctg
      781 gcctagtggc ccggtgtcct ttcccccaaa cccattggct ccgtgttcgc atgcccgaat
      841 acgtccaata cagatgaaag agaagaagga agacgcagaa tgtcggtgaa gatccgcctt
      901 gctcgcggtg gcgcaaagaa gcgcccctat tactacatcg tggtcgccaa cagctcggca
      961 ccacgcgatg gccgctacat cgagcaggtt ggcgcctata atcccatgct gaagaaggac
     1021 gatccgaatc gggtgaagct cgacatcgac cgctgcaagc actggctctc ggtcggcgcc
     1081 cagccgacgg atcgcgtcgc gcgcttcttc gatgcggcgg gcctcatcaa gcggcagccg
     1141 aagcacaacc ccatcaaagg caagccgggc aagaagcgtc tggagcgcga agccgcagag
     1201 gccgagaagg cgaaggaagc caccggcgcg taacgccgcg caaattccct gaaggagctg
     1261 gtgaatgggc gctgctcctg cgcgggtcct gcttggtcgc atccgcgctg cgcatgggat
     1321 ccgcgggcac gtgctcattc acacgtttac cgaggcgcct gaggacattg catcctacgg
     1381 tccgctacag gacgagtccg gagagcggca gttcgaaatc acggtggtcc gcgtcacgcc
     1441 gaaaggtgta gtggcgcgca tctccggcgt cgacgatcgc acctcggccg aggcgctacg
     1501 tggcacacag ctctacgtcg agcgcgaccg cctccctccg accgaggagg acgaatatta
     1561 ccactccgat ctcattgggc tcaccgcctt caagccgaac ggcgagcgaa tgggcgaggt
     1621 gatcgccgtt cacaacttcg gtgctggtga catcctggaa atccggctca agggcaaacg
     1681 gaccacggag atggtcccct tcatgcgtga tttcgttccg gaggtggacg tgtcagcggg
     1741 ccggctcact gtccatatgc cgggggagaa cgaagccgat cgggaccaaa agagctgaac
     1801 tactcaagaa tttcgagctg ggcagggtga tagacttcga tcatcggacc cccgcgccgc
     1861 tcgatccaac cgcggacgag cactcggcgg ctctttagcg caagcaaatc catcccggct
     1921 tctgcgaact ggcgcctgtt cgacggctgt accgtaatgg tgaagtcctg ccgccagtcc
     1981 tcgccaaagt tcaggtaaat ccgtcctcgg acgtccgctg tggccaggac ctctccctcg
     2041 acgagttggt acgtgctccg gaagcggagc agctccctgt ggcgattagc tggccgtatt
     2101 tgatacgacg catgctccca tagccccaaa cgtgcctcac gtgcttgctt ctcggcggca
     2161 acgaggtcat ccaagcaatc gctgctgccc ggcagcgcat aggctcgggc atgaccctgc
     2221 cgcagcatct caccttggag ccataggcgt ttgtcatcgg tgcggacgaa gacctgcgcc
     2281 atcaaccggc catatcgatc ggttcgcctg cccgcgaatc cgagttcgac cgaacggccc
     2341 aggctcagct cctccagtgc cgatttcgct gcctcagcga gcggccaagt cccttcatct
     2401 gcgccggtat ccagcgcccg cggtgccaac gcaccgatca accgcacctg cgagccgtca
     2461 tcaagtgcta acgtttcccc gtcgaggacc cgcacgacgg cacgcaccgg gccaagctcc
     2521 agcccacatc ccggtgcctc tgcgagagct ggctttgcta agaggccgac cagcgccaat
     2581 atcccgagcc agcacctcag gcgaacaaaa cctccttcga ccaaggcacc ccctttggcg
     2641 cttgcaggtc gcccccagct gccctacaca cctattgcgt cgcggttcct tccgagccgc
     2701 tgctgcgcta gtgtcgttcg tggcgcttta gttggccagc cggcagtgag agatccgcgg
     2761 ttgttttgaa gagtggcccc gtagctcagc aggatagagc atcagattcc taatctgagg
     2821 gtcgcaggtt cgagtcctgc cggggtcgcc aattgattca aaggcttacg cgagaggcgt
     2881 ctggcgctgg gcagcaaact gggcagcagc gaagtgtgct catcttg
//
LOCUS       DASUMG010000281         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_839499, whole genome shotgun sequence.
ACCESSION   DASUMG010000281 DASUMG010000000
VERSION     DASUMG010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839499"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFX46_04670"
     CDS             complement(<1..775)
                     /locus_tag="VFX46_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HEX5599905.1"
                     /translation="MTEAVAAQNADAPSRVSAELLESFCRDALRACGADEDTAAAATR
                     AMMHASRLGIDSHGVRLLEHYVTVISGGRVNSRPRMGVVRQTGAVATFDADDGHGALA
                     AYRAMEHAVGLARQYGIGAVAIRNSSHFGAAGAFALAAAEAGMIGFAFCNADRFVRLH
                     GGAARFHGTNPIACAVPVPNQRPWLLDMATSAIPYNKIQLYKSLGRPLPQGVASTEDG
                     EDTTDPHRAAMLAPLGGEFGYKGAGLAGLAEILSAVLSGM"
     gene            complement(772..1515)
                     /locus_tag="VFX46_04675"
     CDS             complement(772..1515)
                     /locus_tag="VFX46_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5599906.1"
                     /translation="MAGRLAGKRAFVTGAGQGIGRAVALAFAREGAHVIAASRTFSKM
                     EDLPSKDPKIKPIELDVTDGPAVRRAIEEAGELDVLFNCAGWVHNGTILDCTEEDWAR
                     SFDQNVTSMFYTIRAALPGMIARGRGSIINVASVASSITGVPNRAAYGASKAAVIGLT
                     KAVARDVIKTGVRCNALCPGTTHSPSLEERIQSAEDPEAARRMFIERQPMGRLGTVEE
                     MAAAALYLASDESAFTTGTIFVVDGGQTL"
     gene            1636..>2444
                     /locus_tag="VFX46_04680"
     CDS             1636..>2444
                     /locus_tag="VFX46_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5599907.1"
                     /translation="MRRRKENERDTQAVARGLGSSPRLYQRALEILESQIENGEWPAE
                     ATLTETGIAARLGISRAPARRALAELEKTGLIARSDTRGYIVVGGRRRGRPRRGDKGS
                     VETTAVKLTPQPSWERIYGTVESEIIARISFASWRVNEAELARHFGVSRTVARDVVSR
                     LQQRGIVQKDDSSRWYAPALTAEHVGELYELRWILEPIALKKAAPNLPEGLLPRLHKN
                     LTDAISRATEIGGETLDQLEHELHVTLLSYCRNRALMQAIAFPQSLIIAHS"
BASE COUNT          482 a          759 c          748 g          455 t
ORIGIN      
        1 tcattccgga aaggacggcg ctcaatattt ccgcaagccc ggctagtcct gctcctttgt
       61 agccgaattc accgccaagc ggcgccagca tggcggctcg atgggggtcg gtcgtgtcct
      121 ccccatcctc ggtagaggca acaccttgag gcaatggcct gccaaggctc ttgtagagct
      181 gaattttgtt gtacggaatg gcgctcgtgg ccatatccaa gagccatggt cgttgatttg
      241 gtactggcac ggcacaggca atcgggttcg tgccatggaa ccgcgcggcg ccgccgtgaa
      301 ggcgcacgaa gcgatcggcg ttgcagaagg caaagccgat cattcccgcc tcggctgccg
      361 ccaacgcgaa tgcgccggcc gcgccgaaat gcgacgaatt gcggatagca acagcgccga
      421 tgccgtattg cctcgcgaga ccgacggcgt gctccattgc tcggtaggcg gcgagcgcgc
      481 catggccatc gtccgcgtcg aaggtcgcaa cggcgccggt ctggcggaca actcccatcc
      541 gaggcctgga attaactcgg ccgccagaaa taaccgttac gtaatgctca agaagtctga
      601 caccatggct atcaatgccg aggcgcgatg catgcatcat cgcacgtgtg gccgctgcgg
      661 ccgtatcttc gtctgcgccg cacgctctga gcgcatcccg acaaaatgat tccagtagtt
      721 ccgccgaaac acggctgggc gcatccgcat tctgagcggc aactgcctcg gtcacagcgt
      781 ctgccctcca tccacgacaa atatggttcc ggtggtgaac gcggattcat cagacgccag
      841 ataaagggca gccgcagcca tctcctccac cgtaccgagc cggcccatcg gctgccgttc
      901 gatgaacatg cgccgagcag cctccggatc ttccgcgctc tggatgcgct cttcgagcga
      961 cggtgagtgg gtcgttcctg ggcagagcgc gttgcagcgc acgcccgttt tgatgacgtc
     1021 cctcgcaacc gccttggtca gccctatgac ggcagccttg ctcgcgccgt aggcggcacg
     1081 gttgggcacg ccggtgatgc tggaggccac agaagctacg ttgatgatgg agccgcggcc
     1141 gcgcgcaatc atgccaggca gcgcggcgcg aatggtgtaa aacatcgagg taacgttctg
     1201 gtcgaagctt ctcgcccagt cctcctcggt gcaatcgaga attgttccgt tgtgcaccca
     1261 gccagcgcaa ttgaagagca cgtcgagctc acccgcttcc tcgattgcgc ggcgcaccgc
     1321 cgggccatcg gtcacatcga gctcaatggg tttgatcttt ggatccttcg acggaagatc
     1381 ctccattttc gagaaggttc ggctcgcagc gatcacatgg gccccttcgc gcgcgaaagc
     1441 cagggcaact gcacgaccga ttccctgccc tgctccagtg acgaacgcac gcttacccgc
     1501 aagccggccg gccatctgca ccactctccc aacaaccagc gtcttctgta tacactagac
     1561 gttgggtttc gatcttggca agatcgaatt tgtcgtcgta cgacaggcag gcgtgagctt
     1621 cgggaatggg cggaagtgcg aagacgcaag gagaacgaga gagacactca agccgttgcc
     1681 agagggcttg gctcctcacc gcgcctttat cagcgcgcgc tcgaaatcct tgagtcacag
     1741 atcgaaaatg gagagtggcc ggccgaagcg acgttgacgg agacaggcat cgccgcccgg
     1801 cttggcatca gccgcgcgcc tgcccgtcga gcgctggccg agctggagaa gacgggcttg
     1861 atcgcgcggt cggatacgcg cggctatatc gttgttggtg ggcgacgccg aggcagacct
     1921 cggcggggag ataagggcag cgttgaaacc acggccgtca agctcacccc tcaaccgagc
     1981 tgggaacgaa tttacggcac ggtggagagc gagatcatcg cgcgcatctc ctttgcatcc
     2041 tggcgcgtga acgaggccga gctcgcccgc cattttggcg tgagccggac tgtggcccgc
     2101 gacgtcgtca gccgtctgca acagcgtggg atcgtccaga aggacgacag ctcgcgatgg
     2161 tatgcgccag ccctgacggc cgagcacgtc ggcgaattgt acgagctgag gtggattctc
     2221 gagccgatcg cgctgaaaaa ggctgcaccg aatttgccgg aaggtctcct tccgcgactt
     2281 cataagaatt tgaccgacgc gatttctcgc gccacggaaa tcggtggtga gacgctcgat
     2341 caactcgagc acgagctgca cgtcacgctg ctgagctatt gccggaaccg ggccctgatg
     2401 caggcgatcg cctttccgca atcgctaatt atcgcgcata gctt
//
LOCUS       DASUMG010000282         4579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_598523, whole genome shotgun sequence.
ACCESSION   DASUMG010000282 DASUMG010000000
VERSION     DASUMG010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598523"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2340
                     /locus_tag="VFX46_04685"
     CDS             <1..2340
                     /locus_tag="VFX46_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEX5599908.1"
                     /translation="EPPVMTRIHAHDGQLIAEYGRERRIFVPINAIPKLVIHAFLSAE
                     DKRFYEHGGLDFIGIARAVIRNLQNPGRRPEGASTITQQVAKNFLLSSEQNYERKLKE
                     AILAIRIERAFSKDKILELYLNEIYLGIGSYGVAAAALNYFNKELDDLTPEEAAYLAA
                     LPKAPNNYHPFRKTREATIRRNWVLDQMVENGYLSREEAEQAKAKPLQVNLRPFGAHI
                     FAAEFFAEEVRRTLLARYGEEKLYGGGLSVRTTLDPELQRHARKALIDGLVEFDRERG
                     WRGPVDHIDVSGDWGAKLAEMNVPSDIDPWRLGVVLEVEAAKIIVGLRPTREPDGKIS
                     PKREAVEISFEEMKWAGKFRATDRPKGPAPKKPSDVVQVGDVVYVSPKDPNDPTGAWS
                     LMQIPEVGGGLVAMDPHTGRVLAVVGGFSFSISQFDRAIQARRQPGSSFKPLVYAAAL
                     DNGYKPTSIVLDAPIEIDQGPGQEVWRPRNYNKEASYGPSTLRIGIEKSRNQMTARLA
                     QELGMPIIVEYSKRFGAYDDLLPVLAMSLGAGETTLLRMTAAYCVFANGGKQVRPTLI
                     DRIQDRWGRTIWRHDQRECVGCNADEWRGQEEPEIPDDRRQVIDPYTAFQMTSILEGV
                     VQRGTATIVKKILPGTPLAGKTGTTNESKDAWFVGYSPDLVVGVYVGYDTPKPMGKSA
                     TGGQIAAPIFANFMKLALNGTKVPFRAPPGIRSIPVNRYTGIRASPGDSNVIWEYFKP
                     DEEPDDPNSFLGYETSSYSDSDRTYPPARNVISGFGNVY"
     gene            2532..3657
                     /gene="prfB"
                     /locus_tag="VFX46_04690"
     CDS             join(2532..2597,2599..3657)
                     /gene="prfB"
                     /locus_tag="VFX46_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_122381344.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX5599909.1"
                     /translation="MRAEPQKHVDAIRRSLSLLRRHFDWDTAEARLAELNARAEDPNL
                     WNDPRAAREIMRERDSLDRKISSYKQLERELEDAITLIELGEAEGDEETIAEGEAALK
                     ALSELAQKREIEALLSGEADGNDAYLEVHAGAGGTESQDWAEMLVRMYIRWAEDHGFE
                     VTMVEASPGEEAGLKSATLEIKGENAYGWLKTESGVHRLVRISPFDANARRHTSFASV
                     WVYPKVDDTIEIDIKESDVRIDTYRASGAGGQHVNTTDSAVRITHLPTGIVVACQQER
                     SQIKNRAIAWAMLRARLYDLELKKREEKANAEAAAKTEIGWGHQIRSYVLQPYQLVKD
                     LRTGVESSNPQAVLDGDLDPFIEAALAQRLKDEQRAHASQ"
     gene            3806..>4579
                     /locus_tag="VFX46_04695"
     CDS             3806..>4579
                     /locus_tag="VFX46_04695"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX5599910.1"
                     /translation="MTTATRAACVIGWPAKHSRSPKMHRWWLKHYGIDGDYRFEEVPS
                     EEFPAFIANLRGNGYIGANITIPHKEAAFKLTDPDERARAVGASNTLWYGDDGVLRST
                     NSDVDGFLAALDVSAPGWSETGKHAVVVGAGGAGRAAVWGLLERGIRRIHVVNRTFDR
                     AVALAERYGDRVVPTRWEDRNKVLDGAGLLANATTLGFRGNPELDIELSGMAPDAVVI
                     DINYVPLETPLLARARQLGLKTADGLDMLLYQACTGFELW"
BASE COUNT          935 a         1380 c         1452 g          812 t
ORIGIN      
        1 gagccgcccg tgatgacgcg cattcatgcg cacgacggac agctcatcgc cgagtacggg
       61 cgtgagcggc gcatcttcgt gccgatcaac gccataccca agctcgtgat ccatgctttt
      121 ctgtctgctg aggacaagcg cttctacgag catggcgggc tagacttcat cggcatcgca
      181 cgcgctgtca tcagaaacct gcagaatcct gggcgacgtc ccgaaggtgc atcgaccatc
      241 acccagcagg tcgccaagaa cttccttctt tccagcgagc agaactacga acgcaagctg
      301 aaggaggcga tcctcgcgat ccgcatcgag cgggccttct cgaaggataa aatcctcgag
      361 ctatacctga acgagattta cctgggcatc ggctcctacg gcgtggccgc agccgccctc
      421 aactacttca acaaggagct ggacgatctg acgccagaag aggcggccta cctggcggcg
      481 ctgccaaagg ctcccaacaa ttaccatccc ttccgcaaga ctcgcgaagc caccattcgg
      541 cggaattggg tgctcgatca gatggtcgag aacggctacc tcagccgcga ggaggcggag
      601 caggccaagg ccaagccttt gcaggtcaac ctgcgtccct ttggggcgca catcttcgct
      661 gccgagttct tcgcggagga ggtgcgccgg acgcttcttg cgcgctacgg tgaggagaag
      721 ctgtacggtg gcgggctttc ggtacgcacc acgctcgatc ccgagctgca gcgccacgcg
      781 cgcaaagctt tgatcgatgg cctcgttgag ttcgatcgcg agcgcggctg gcgcggtccc
      841 gtcgaccata tcgacgtcag cggtgactgg ggtgccaagc tggcggagat gaacgtcccc
      901 tcggacattg atccgtggcg gttgggcgtc gtcctcgaag tggaagcggc caagatcatc
      961 gtcgggctca ggcccacgcg cgagccggac gggaagatct ccccgaaacg ggaagccgta
     1021 gaaatctcct tcgaggaaat gaagtgggcc ggcaagttcc gtgccaccga tcgcccgaag
     1081 gggccagcac caaagaagcc gtcggatgtc gttcaagtgg gggacgtggt ctacgtgtcg
     1141 cccaaggatc cgaacgatcc caccggcgcc tggagcttga tgcagatccc ggaggttggt
     1201 ggcggcctcg tggcgatgga cccgcacacg ggccgcgtgc tggcagttgt tggtggcttc
     1261 tccttctcga tcagccagtt cgaccgcgct atccaggcga ggcgccagcc agggtcctcg
     1321 ttcaagcctc tcgtttatgc cgctgccttg gataacggct acaagcccac gagcattgtg
     1381 ctcgatgcgc ccatcgagat cgaccagggg cctgggcagg aggtctggcg cccgcggaac
     1441 tacaacaagg aagcttcgta cggcccctcc accctacgga tcggcatcga gaagtcccgt
     1501 aaccagatga cggcacgcct ggcgcaagag cttgggatgc cgatcatcgt cgaatactcc
     1561 aagcggttcg gcgcctatga tgatctgctc cccgtgctcg cgatgtcgtt gggtgcggga
     1621 gagacgacac tgctccgaat gacagccgcg tactgcgtgt ttgccaatgg cggcaagcaa
     1681 gtcagaccga ccctgatcga ccgaatccag gaccgctggg gccgaacgat ttggcggcac
     1741 gatcagcgcg agtgcgtcgg ttgtaacgcg gatgaatggc gcggtcagga ggagcccgaa
     1801 atcccggacg accggaggca ggtcatagac ccctacacgg cgttccagat gacctcgatt
     1861 cttgaaggcg tcgtgcaaag gggcactgcg acgatcgtga agaagatact gcccggcacg
     1921 cctctggcgg gaaagacagg cacgacgaac gaatcgaagg acgcgtggtt cgtcggatac
     1981 tcgccggacc tcgtcgttgg cgtctacgtc gggtacgaca cgcccaagcc catgggtaag
     2041 tccgcgaccg gtggacagat tgcggcgccg atcttcgcca acttcatgaa gctcgcgctc
     2101 aacggcacga aggtcccgtt ccgcgctccg ccaggcattc gctcgatacc ggtcaaccgc
     2161 tacacgggca tccgagcatc gccaggcgac agcaatgtca tctgggagta cttcaaaccg
     2221 gacgaggagc cggatgaccc gaactcgttc ctcggttatg aaacgagctc atacagcgat
     2281 agcgacagaa catacccgcc ggcccgcaac gtgatttctg gctttgggaa tgtttattga
     2341 taggcgcgcc tgtcacggcg cgccctgagg gctcggcttc agccaagttt tttcgttgct
     2401 cgcgctaact cgtgagtgag ggtggccaat cgcatttaca tcgcagcgca gcacacttat
     2461 atcgtagaca catcagagac ctgtcagaca catccaatcc cgaccgaatc ctgacatttg
     2521 gaggtttgcc gatgcgggcg gagccgcaga agcacgttga tgccattcgc cggtcactgt
     2581 ctctcctacg gaggcatctt tgactgggat acagccgaag cacgactagc cgaactcaac
     2641 gcacgggctg aggacccgaa cctttggaac gatcctcgcg ccgcccgcga aatcatgcgc
     2701 gagcgtgatt cgctcgatcg caagatttcg tcctacaagc agctggagcg ggagctcgag
     2761 gacgcaatca cactgatcga gctcggcgag gctgaaggcg acgaggagac gatcgccgaa
     2821 ggcgaggcgg cgctcaaggc gctgagtgag cttgcccaga agcgcgagat cgaggccttg
     2881 ctctcgggcg aggcggacgg caacgacgcc tatctcgagg ttcacgcggg tgctggcggt
     2941 acggaaagcc aggactgggc cgagatgctc gtgcggatgt atatccggtg ggccgaggac
     3001 catgggtttg aagtgaccat ggtcgaggcg agcccgggcg aggaagccgg cctgaagtca
     3061 gccaccctcg agatcaaggg tgagaacgca tacggctggc tgaagaccga atctggcgtg
     3121 cacaggctgg tgcgcatctc gccgttcgat gccaacgccc ggcgacacac cagcttcgcg
     3181 tccgtctggg tctatccgaa ggtcgacgac acgatcgaga tcgacatcaa ggagagcgac
     3241 gtccggatcg acacctatcg ggcgtctggt gccggtggcc agcacgtcaa cacgaccgac
     3301 tcggcggtgc gcatcacgca cctgcccacg gggatcgtcg tggcctgcca gcaggagcgt
     3361 tcgcagatca agaaccgggc cattgcctgg gccatgcttc gggccaggct ttacgacctc
     3421 gagctgaaaa agcgcgagga gaaggccaat gcagaggcgg ccgccaagac ggaaattggc
     3481 tgggggcacc agatccgctc atacgtgctg caaccgtacc agctcgtgaa ggacctgcgg
     3541 acgggcgtgg agagcagcaa tccccaggcc gtgctcgacg gtgacttgga tcctttcatc
     3601 gaggcggcgc ttgctcagcg attgaaggac gagcaacggg cgcacgccag ccagtaattc
     3661 agcagtaggc cgcctttgct aactctacca actttcgggc cgggctcctg tacaatggtg
     3721 cccggctcga gtgtggtatc ggcgagtaag agtgctggct ccgccactcg tgctatcatc
     3781 ccggcgcctg caacgaggat aagcaatgac cactgcaacg agagccgcat gcgtgatcgg
     3841 ctggccggcg aagcactccc gctcgccgaa gatgcatcgc tggtggctga agcattacgg
     3901 catcgacggt gattatcgtt tcgaggaagt gccgtccgag gagttccccg ccttcatcgc
     3961 caacctccgc ggcaacggct acatcggcgc caacattaca atcccccaca aagaggcagc
     4021 attcaaactg acggatcccg atgagcgtgc gcgcgccgtg ggcgcgtcga atacgctgtg
     4081 gtatggcgat gatggcgtgc tgcgctcgac gaattcggac gtcgacggct tcctcgctgc
     4141 attggacgtc tcagctccag gttggagcga aacaggcaag catgcggtgg tggttggcgc
     4201 cggtggcgcg gggagagccg cggtctgggg cctgctggag cggggcattc ggcgcatcca
     4261 tgtcgtcaac cggaccttcg atcgtgcggt ggccctagcc gagcgctatg gcgatcgggt
     4321 cgtgcccacc aggtgggaag atcggaacaa agtccttgat ggtgcaggcc tcttggccaa
     4381 tgccacaacg ttgggcttca gaggcaatcc cgaactcgac atcgagcttt ccggcatggc
     4441 gccggatgcc gttgtcatcg acatcaatta cgttccgctt gaaacgccgc tattggcccg
     4501 cgcgcggcag ctcggcctca aaacggccga tggactcgac atgctgcttt atcaggcatg
     4561 cactggattc gagttgtgg
//
LOCUS       DASUMG010000283         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_839638, whole genome shotgun sequence.
ACCESSION   DASUMG010000283 DASUMG010000000
VERSION     DASUMG010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839638"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1066)
                     /locus_tag="VFX46_04700"
     CDS             complement(32..1066)
                     /locus_tag="VFX46_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5599911.1"
                     /translation="MDMCRSSRQVFCAVLGAIFFVCSSASIPAATLDEVRSRGHLLCG
                     VAEGLMGFSHLDARGVWSGLEVDFCAALAAAIFGDRNAVKYRPLTLAERFSALRDGEV
                     DVLARSAPWTLSRDMDLGARYVDTLFFDGQAIMVRRAQGVTSVLELSGASICVMNAGS
                     AQQGLEEYFSQRGMRFTAVTFDKWDAAVEAYLNERCTALTADLTALALVRAAIDQTGE
                     HQLLPELLTKEPLGPAVKQGDPQWFGIVRWVLYALIAAEEHGVSRANVEAMRQSPIAA
                     VRRLLGTDGDLGASLGLSRTWAYDVIRQVGNYGELFERNVGMRSPLKLERRINDLWSK
                     GGLMHAPPFR"
     gene            complement(1178..>2292)
                     /locus_tag="VFX46_04705"
     CDS             complement(1178..>2292)
                     /locus_tag="VFX46_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HEX5599912.1"
                     /translation="HDFHAHPEVLYDVHRTAGIVAEKLKAWGCDEVVTGIGRTGVVGV
                     IRGRSNNSGKVIGLRADMDALPIQEETGLPYASTIPGRMHACGHDGHTTMLLGAARYL
                     AETRNFDGTVVLIFQPAEEGGAGAKAMIDDGLMDRWGIQEVYGLHNQPGLPVGSFALR
                     PGPLMAASDRIYITIHGKGGHAAKPHCCIDPLLAGAHVVTALQSIVSRNVDPLESGVV
                     SICMFHAGDTQNVIPATAKLTGTARSLQPHIRDLLEKRIIEVTKATAAMFGAEADVVY
                     KRDYPVTRNHERETQFAASVAAAVVGPDKVNTSAPPVMGGEDFSFMLEARPGAFIFMG
                     NGDSAGLHHPAYNFNDDAIPYGVSYWVRLVETAMPA"
BASE COUNT          447 a          740 c          691 g          414 t
ORIGIN      
        1 gttacacgcg ttttcgtacg tcggctgatc gtcacctgaa tggtggcgcg tgcatcaggc
       61 cgcctttgga ccacagatcg ttgatgcgac gttccagctt cagcggtgac cgcatgccga
      121 cattcctctc gaacagctcg ccgtaattgc cgacctgccg aatgacatcg taagcccacg
      181 tacggctgag cccgagcgac gcgcctaggt cgccatccgt tccgagcaag cgtctcacgg
      241 ctgcgatcgg cgattgacgc atcgcctcca cgttcgcccg tgacacgccg tgctcctcgg
      301 cggcgatcag cgcgtacagc acccagcgga cgatgccgaa ccactgagga tcaccctgtt
      361 tcaccgcggg accgagcggt tccttcgtca gcagctcagg tagcagctga tgctcgccgg
      421 tttgatcgat tgcagcgcgg acgagggcta gcgccgtcag atcggcagtg agcgcagtgc
      481 agcgctcgtt gagataggct tcgacagctg cgtcccattt atcgaacgta accgcggtga
      541 agcgcatgcc gcgctggctg aagtactcct cgaggccttg ctgcgcggag cccgcattca
      601 tcacgcaaat gctcgcacca ctgagctcga gcacgctcgt tacgccttga gcccggcgca
      661 ccataattgc ctggccatcg aagaagagcg tatcgacgta ccgtgcgccg agatccatat
      721 cgcggctcaa cgtccacggt gcactgcgag ccagaacatc aacctctccg tcacgcagcg
      781 ccgaaaagcg ctctgccaag gtgagcgggc ggtatttcac ggcgttccga tcgccgaaga
      841 ttgccgccgc aagagccgcg cagaaatcca cctccaggcc cgaccagacg cctcgcgcat
      901 cgagatgcga aaatcccatc aggccttcgg cgacgccgca caacaggtgc ccgcgcgacc
      961 ggacttcgtc gagcgttgcg gccgggatgc tcgcgctgct gcaaacaaag aaaatggcgc
     1021 cgagaaccgc gcagaatacc tgacgtgacg atctgcacat atccattcga aaagccgctg
     1081 atgagtgatt gccgtacccg gcagctggcg gtcagccaag gcggctcacc cgcgcagatc
     1141 tgccccggtg tgcacccccc aggcttacgc cgtcagctca cgcaggcatg gctgtctcga
     1201 ccagacgcac ccaataggag acgccgtagg ggatcgcatc gtcgttgaag ttgtacgctg
     1261 ggtggtggag gccggcgctg tcgccattgc ccatgaaaat gaaggcgcca ggccgcgctt
     1321 ccagcatgaa cgagaaatcc tctccgccca taaccggcgg cgcggacgta ttcaccttgt
     1381 cagggcccac gacggcggcc gcaacagaag cagcgaactg cgtctcacgt tcgtgattgc
     1441 gggtcaccgg ataatcgcgc ttgtaaacga cgtctgcttc ggcaccgaac atggcggccg
     1501 tcgctttcgt gacctcgata atccgcttct cgagcaggtc gcggatgtgg ggctgtagcg
     1561 agcgcgcggt gccggtcagt ttcgccgtcg ccggaataac attctgcgta tcgccggcgt
     1621 ggaacatgca gatcgacacg acgccagact ccaagggatc gacgttcctg gagacgatgg
     1681 actgcaatgc ggtgacgaca tgcgcaccgg cgagcagcgg gtcgatgcaa caatgcggct
     1741 tggcggcgtg cccccctttg ccgtggatcg tgatgtagat ccggtccgag gcggccatta
     1801 gcggacctgg cctgagagca aagctgccga caggaagccc tggttggttg tggagaccgt
     1861 agacctcctg aatgccccag cgatccatga ggccgtcgtc gatcatcgcc tttgcgccag
     1921 cgccgccttc ctcagcgggt tgaaagatca agacaaccgt gccgtcgaaa ttccgtgttt
     1981 cagcaaggta acgggcggca ccaagcagca tcgtggtgtg tccgtcatgc ccgcaggcat
     2041 gcattcggcc ggggattgtc gaagcgtaag gcaggcctgt ctcctcttga atcggtaggg
     2101 cgtccatgtc ggccctcagc ccgataacct tgcctgagtt gttgctccga ccacggatca
     2161 ccccgacgac gcccgttcgg ccgatgccgg tgacaacctc gtcgcagccc caggccttca
     2221 acttctccgc cacgatgcca gccgtccgat gcacgtcgta aagaacctcg ggatgtgcgt
     2281 ggaagtcgtg gc
//
LOCUS       DASUMG010000284         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_117694, whole genome shotgun sequence.
ACCESSION   DASUMG010000284 DASUMG010000000
VERSION     DASUMG010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117694"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="VFX46_04710"
     CDS             complement(<1..832)
                     /locus_tag="VFX46_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5599913.1"
                     /translation="MALSTGVGSLSAGSETRHHALSLVGEPAYGPDFKHFDWVNPDAP
                     KGGTVRQWAMGSFDSLNPFSVQGSTAAGVGLIYDTLMTSSPDEPSTEYGLVAEWVSFP
                     DDFSSATFGLRPEARFHDGEPIKPEDVIFSLEALKKAHPHYNAYYRNVVRAEKTGDHE
                     VTFTFDQKGNRELPQIVGQLPVLPKHFWEATGANGQPRDLSRSTLEIPLGSGPYKIKS
                     FDPGRSIVYERVKDWWAKDLPVSKGQWNFDEFVFTYFRDRVPASEAFKVGQIDYWQET
                     S"
     gene            complement(1110..1661)
                     /locus_tag="VFX46_04715"
     CDS             complement(1110..1661)
                     /locus_tag="VFX46_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX5599914.1"
                     /translation="MDTRELNKIAGAVIAALLLMFGSATFIDIQMSHKRLAKPGYELP
                     VETTTAAESSQTSAAAGLNFEEIASLLQNASADSGQAAFKRCSTCHTPDKGGRNMTGP
                     NLWGIVGRPKGSQEGFNYSAALKEKGGEWGYEELANFIHNPRSYIPGNKMAFAGIRDN
                     GEIADLLAYLRTLSDNPPPLPSN"
     gene            1838..2578
                     /locus_tag="VFX46_04720"
     CDS             1838..2578
                     /locus_tag="VFX46_04720"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX5599915.1"
                     /translation="MTRTLVIIPARMQATRLPGKPMADIHGKPMIVHVWERARAADVG
                     RVVVATDAEEIASAIRDLGGEAVLTRADHASGSDRVYEALQKLDATGAADFIINLQGD
                     LPTLEPHLVRECLVPLLGPGPDIATLAAEIREPDERVAPSVVKVVGTSIGCDGARQLR
                     ALYFTRATAPFGEGPLYHHIGIYGFRRAALERFVALPPSPLELREKLEQLRALENGMR
                     IDVTLVDTVPLGVDTPADLERAREMLAA"
BASE COUNT          459 a          809 c          812 g          523 t
ORIGIN      
        1 cgctcgtctc ttgccagtag tcgatttgac cgactttgaa ggcctcggat gccggcaccc
       61 gatctcggaa ataggtgaag acgaactcgt cgaagttcca ctggcccttg gagacgggca
      121 ggtccttcgc ccaccagtcc ttcacccgct cgtagacgat gctgcgacct ggatcgaacg
      181 acttgatctt atagggcccg gagccgagcg ggatttctaa ggtgctgcga gacaggtccc
      241 gcggctggcc gttggcgccg gtcgcttccc agaagtgctt gggcaacacc gggagctggc
      301 cgacgatctg cggcagctcg cgattccctt tctggtcgaa ggtgaaggtc acctcatggt
      361 cgccggtttt ctcggcgcgg acgacgttac ggtagtaagc gttgtagtgc gggtgcgcct
      421 tcttgagggc ttcgagcgag aagatgacgt cttccggctt gatcggctcg ccgtcgtgaa
      481 agcgggcttc cgggcgtagg ccgaaggtgg cgctcgaaaa gtcgtcggga aaggagaccc
      541 attcggccac aagaccgtat tcggtcgagg gttcgtctgg gctcgaggtc atcaatgtat
      601 catagatgag gccgacgccc gccgccgtgc tgccttgcac ggagaagggg ttgaggctgt
      661 cgaaggagcc catggcccac tggcgcacgg tgccgccctt gggcgcatcc ggattgaccc
      721 agtcgaagtg tttgaaatcc ggtccgtatg ctggctcgcc gacgagcgaa agggcgtgat
      781 gccgtgtctc gctgcctgcc gaaagactac caacaccggt gctaagggcc attgccgctg
      841 cggcgagcgc caaaagacct ctgcctatgc taccgttgac gcgtgtggcc acacgatact
      901 cccgcctaag tggtggaaca ctggcccatg cctcgccggg gcacgggcca acaaatgagg
      961 cctccgtggc tccggagcaa ccatcgacgc cgttataaca gcaaaagcga ccccgccaat
     1021 ctatggccgg gtcgcacacg gtcgtagctc gatcacaatc tggccaatag ccccgcgagc
     1081 cgcgatgcta gggcccgctt gagcatcact taattgcttg gcagcggcgg cggattgtcg
     1141 ctcaacgtcc gcaggtaggc gagcagatcg gcgatttcgc cattatccct gatgccggca
     1201 aacgccatct tgttgccagg gatgtagctg cgcgggttgt ggatgaagtt ggccagctcc
     1261 tcgtaacccc actcgccgcc cttttccttc agggctgccg agtagttgaa gccttcctgc
     1321 gaacctttcg gtctgccgac aatgccccag aggttaggcc ctgtcatgtt gcggccaccc
     1381 ttgtcaggcg tgtggcacgt cgagcagcgc ttgaacgccg cctgtccgct atcggcgcta
     1441 gcgttctgca gcagactggc aatttcctcg aagttcagac cagcagctgc ggaggtttgg
     1501 gacgactccg cggcagtcgt ggtctcgacc ggcagctcgt atcctggctt ggccagccgc
     1561 ttatggctca tctggatatc gatgaatgtc gccgatccaa acattaggag gagcgctgcg
     1621 attacagccc cggcgatctt gttcaactcc ctggtatcca tcgaatcccc agccctctgc
     1681 ccgcgccagt cgcttcttat gactcgtgcc tcgatcggcg gccgactata gaaagtccca
     1741 atcttcatgc aaccggggtg gcggttcggt ccagtcgctc gtgccgagac caattgtcac
     1801 tcgccaaaac gtcagtcctg ctcgaagctg cacactcatg acccgcacgc tcgtcatcat
     1861 tccggcccgt atgcaggcca cgcgtcttcc cggcaagccg atggcggaca tccacggcaa
     1921 gccgatgatc gtccacgtct gggagcgtgc gcgggcggcc gatgtcggcc gcgttgtcgt
     1981 cgcgacagac gcggaggaga tcgcttccgc tattcgtgat ttgggcgggg aggctgttct
     2041 gacgcgtgcg gatcatgcgt ccggctcgga tcgcgtgtac gaagcattgc agaaactcga
     2101 cgcaaccggc gctgccgatt tcataatcaa tctgcagggg gacctgccaa cgctcgagcc
     2161 gcacctggtt cgcgaatgcc tggtgccgct gttgggcccc gggccggata ttgccaccct
     2221 cgcagccgaa atcagggagc ccgacgaaag ggtcgctccg agcgtcgtga aggtcgttgg
     2281 gacctcgata gggtgtgatg gggcgcgtca gctccgcgcg ctctacttca cccgtgcgac
     2341 ggcccccttc ggcgaaggcc cgctctatca tcatatcggc atctatggct tccgccgtgc
     2401 ggcgctggag cggttcgtag ccctcccgcc gtcaccgctc gagctgcgtg agaagcttga
     2461 gcagctccgc gcgctcgaga acggcatgcg catcgacgtg acgctcgttg acaccgttcc
     2521 gctgggtgtc gatactcccg ctgatctgga gagggcgcgc gagatgcttg cggcttgaca
     2581 cgagccgagt tctgagccga aac
//
LOCUS       DASUMG010000285         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_117715, whole genome shotgun sequence.
ACCESSION   DASUMG010000285 DASUMG010000000
VERSION     DASUMG010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117715"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFX46_04725"
     CDS             complement(<1..177)
                     /locus_tag="VFX46_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5599916.1"
                     /translation="MRDQINQAIKEAMKAGDKRRLATLRLISAAIKDRDIAAQIDSAG
                     QPTGTDKISDAEVLQ"
     gene            413..1579
                     /gene="carA"
                     /locus_tag="VFX46_04730"
     CDS             413..1579
                     /gene="carA"
                     /locus_tag="VFX46_04730"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5599917.1"
                     /translation="MTARLVLADGTVITGRGLGAVGSAVGEVCFNTAMTGYQEILTDP
                     SYAGQIITFTFPHIGNVGTNPEDTETSNLAMRSGVRGCVLRADITSPSHYRAVEHLDA
                     WLKARGIIAICGVDTRALTARIRAQGMPNAVIAHEPSGVFDIEKLKAEARAWPGLVGL
                     DLVPEVTTGQSYTWDEMRWVWGKGYPRQLNPEFHVVAVDYGLKRNILRSLASAGCRVT
                     VVPATTTAEEILERKPDGVFLSNGPGDPAATGKYAVPEIQRLIDSGLPLFGICLGHQL
                     LGLAVGARTVKMHQGHHGANHPVKDFTTGKVEITSMNHGFAVDRDTLPADVEETHVSL
                     FDGSNCGLRLKGRPVFSVQYHPEASPGPQDSHYLFIRFVNMMRERKGLPPLKER"
     gene            1581..2594
                     /locus_tag="VFX46_04735"
     CDS             1581..2594
                     /locus_tag="VFX46_04735"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX5599918.1"
                     /translation="MAVGRCAAVVLSLGLLIFPAEGASAQSGSATQTEGRVSAQDQAL
                     VGTIWSAREGRAVSREELLEGLSSTPLILLGEVHDNPEHHSLRAWIIGELATRRSASG
                     ERGPAVVFEHIRADQQSLVDDFLASDERADAGALLQKLDWDRSGWPSAAMFSPLFEQA
                     LKFRLPIFAGNTPAEAIRKVAREGLASLLDEERSRLGLDQQLEPHLYAALIKDIEENH
                     CGLVPESAFPPMALAQQYRDAHLAERMISAMAANGAAVLLAGNGHVRSDRGVPWHLRR
                     RAPGSNIGVVTFVEADPGITEAAPYVPLDPDGQPATDYVWITSRAERPDPCEEMRQRI
                     SRP"
BASE COUNT          506 a          871 c          885 g          557 t
ORIGIN      
        1 ctgcagaacc tccgcgtcgg aaatcttgtc agtgccggtg ggctgcccgg cgctgtcgat
       61 ctgagctgca atgtcgcgat ccttgatggc cgcggagatg agccgcaagg tagcaagccg
      121 gcgcttgtca cccgctttca tggcctcctt gatggcctgg ttgatctgat cgcgcatgac
      181 acctcctcct agtattggcg tgatgtacgc ccccagtggc atcgtcgcaa ggctggcacc
      241 cattgattga cgcgttccgc cgcctcacat aggttgccgg tctgcaaaca gcttattgcg
      301 atgcagcgag cggtgccctt tgccgatgcg gccgcgccgc ctggagccga tcatgacagc
      361 gataacccaa tccatccgcc gcagtgcgcc cgcgccctgg gccgagccgc tgatgactgc
      421 ccgccttgtg cttgcagacg gcaccgtgat caccggccgt gggctgggag cagtcggatc
      481 ggcagtcggc gaggtctgct tcaataccgc catgaccggc taccaggaga tcctcaccga
      541 tcccagttac gccgggcaga tcatcacctt taccttcccg cacatcggca atgtcggcac
      601 caatcccgag gataccgaga cctccaacct cgccatgcgc tctggcgtgc gcggttgcgt
      661 gctgcgggcc gatattacgt ctccgtccca ttaccgcgcg gttgagcatc tcgatgcttg
      721 gctcaaggcg cgtggcatca tcgcgatttg cggggtcgat acgcgggcgt tgaccgcacg
      781 gattcgcgct cagggcatgc ccaatgcggt catcgcgcac gagccgtctg gggtctttga
      841 catcgagaag ctgaaggcgg aggcacgggc ctggccgggt ctcgtggggc ttgacctcgt
      901 gccggaggtg acgaccggcc agagctacac gtgggacgag atgcgctggg tgtggggcaa
      961 aggctatccc cgccagctca atcccgagtt tcacgtcgtc gcggtcgact atggcctgaa
     1021 acggaacatc cttcgctcgc tcgcgagcgc cgggtgtcgc gtgacggtgg ttcctgccac
     1081 aacgaccgcg gaagagatcc tcgagcggaa acccgatgga gtgtttctct cgaacggacc
     1141 aggagatcca gcggcaacgg gcaagtatgc tgtgcctgag atccagcggc tgatcgactc
     1201 gggactgccc ttgtttggca tctgcctcgg ccatcagctc cttggccttg ccgttggagc
     1261 acgcaccgtg aagatgcacc aaggccacca cggagcgaac cacccggtaa aggacttcac
     1321 gaccggcaag gtcgagatca cgtccatgaa ccacggcttc gctgtagacc gcgatacgct
     1381 gccagccgat gtcgaggaga cgcacgtctc gctgttcgac gggagcaatt gcggcttgcg
     1441 gctcaagggc cggccggtgt tctcagtcca gtatcaccca gaagcctcgc ctggcccgca
     1501 ggacagccac tatctgttca tccgcttcgt gaacatgatg cgcgagcgca agggcttgcc
     1561 gccgctcaag gagcgttagt atggccgtgg ggcgctgcgc tgctgtggtg ctgagccttg
     1621 gcctgctaat cttcccggcc gaaggtgcca gcgcgcagag tggcagtgca acccaaacgg
     1681 agggtcgagt gtcggctcag gaccaggcgc tcgtgggtac gatctggtcg gcccgggaag
     1741 gccgtgccgt ctcgcgagaa gaattgctgg aaggtctctc gagcacgccg ctcattctgc
     1801 tcggtgaagt tcacgacaat cccgagcacc acagccttcg cgcatggata atcggcgagc
     1861 ttgccacccg tcgaagcgcg tcgggcgaga ggggcccagc tgtggtgttc gagcatattc
     1921 gcgccgatca gcagagcctg gttgatgact tccttgcttc cgacgaacga gcagacgcgg
     1981 gcgcgcttct tcagaagctc gactgggatc ggagcggctg gccctcggcg gctatgtttt
     2041 caccactgtt cgagcaggcg ctgaaatttc ggctgcccat cttcgctggc aacaccccgg
     2101 ccgaggcaat ccgaaaggtg gcacgtgaag ggctggcgtc tctgctggat gaggagcgat
     2161 cccgcctagg gctcgaccag caacttgagc cgcacctgta cgcggctctc atcaaggata
     2221 tcgaggaaaa ccattgcgga ttggtgcctg aaagcgcgtt cccgccgatg gcgctcgccc
     2281 agcaatatcg cgatgcgcat cttgcggaga ggatgataag cgcgatggcg gcaaacggcg
     2341 ccgcggtctt gttggcgggc aatggtcacg ttcgttcgga ccgcggcgtg ccctggcatt
     2401 tgcgccgccg tgctccgggg tcaaatattg gtgtggttac cttcgtagaa gcggaccccg
     2461 gcataacaga agctgcccct tatgtgccgc tcgatcctga cgggcagcca gcgaccgatt
     2521 atgtatggat cacctcacgc gcggagcggc ctgacccgtg cgaggaaatg cgacagcgca
     2581 tttcgcgtcc ctgaagctga gcactcgatc agacgcttac atttgatttt ggtgcagaaa
     2641 ctgcgtggcg cgaattgagg tcgtttacct tccgcgccac tccgatcttc tgatctgttc
     2701 ttccgagccg cgctgctcgg tgcgtctttt gaggcggggc gacagaaaaa tgcgcgccga
     2761 gctcttcaac gccctttaca tgccgagcaa ccgatcttat ttcggcgact cattcgaat
//
LOCUS       DASUMG010000286         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_519368, whole genome shotgun sequence.
ACCESSION   DASUMG010000286 DASUMG010000000
VERSION     DASUMG010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519368"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1554
                     /gene="otsA"
                     /locus_tag="VFX46_04740"
     CDS             97..1554
                     /gene="otsA"
                     /locus_tag="VFX46_04740"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HEX5599919.1"
                     /translation="MVARLIVISNRVAVPESASEFSAGGLAVAVQAALSGRDGIWFGW
                     SGRVTEEPDHEPTIRKVGGTTYIVLDLAPGDFNEYYNGFANRVLWPILHYRLDLAEFS
                     RSDLSGYQKVNNYFADHLSRILKPDDIVWVHDYHLMPLARLLRERGHQNRIGFFLHVP
                     CPPPDILTALPKHREIIGSLVAFDLIGLQTENDCENLSRYLLRMGARRAPDAYHLEAN
                     GRRVTLGAFPVGIETARFERTARRAAATALIKETAESLAGRAMVMSVDRLDYSKGLPN
                     RMAAIERFLSMYPEWRGRVTFLQIAPKTRSEIQEYAEIDRQVSTLAGQINGLHGDVTW
                     TPIRYVNRVYSRAALAGLYRLAKVGLVTPLRDGMNLVAKEYVAAQNPLDPGVLVLSQF
                     AGAAAELDGALIVNPHEPEAVAAAIRFALEMPLDARQARHQRMMEVLRRNDIHAWASK
                     YLATLEQTRPTPATISATAAARHLPPGSIAASASR"
     gene            1673..>2099
                     /locus_tag="VFX46_04745"
     CDS             1673..>2099
                     /locus_tag="VFX46_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599920.1"
                     /translation="MCARSPFTFAQRLIFYGLSVAVPVLAICGLLVYQYTAQQYRAIE
                     EETLAAAQELARDLEREIQVQLLLLEALIGVLGPEMDLARLHQNARRALVPHGAHVVV
                     YDVGGWQILNTSVPMGDPLPVSYDPVRIKAIVERGQPYVS"
BASE COUNT          409 a          653 c          609 g          428 t
ORIGIN      
        1 agtaaccgga cgcctttggg gtaatctgcc gcagacctat tcgatggcgg gaattgtcat
       61 caccgccaag cggctttcga agggttggga gcaagcatgg tcgctcgcct cattgtgata
      121 tctaaccgtg tcgctgtacc ggagagtgca agcgaattct ctgccggtgg gcttgcagtg
      181 gctgttcaag cagccctcag tgggcgggat ggtatctggt ttggctggag cggaagagtc
      241 actgaggagc cagaccatga gccaacgata aggaaagttg gtggtaccac atacattgtt
      301 ctcgatctcg cccctggcga cttcaacgag tactacaacg gatttgccaa ccgggtctta
      361 tggcccatcc tgcattaccg attggacctt gctgaattct cacgctcgga tctttcaggc
      421 tatcagaaag tcaacaacta ctttgccgac cacctttctc gaatactcaa gccggacgat
      481 atcgtctggg tccacgatta tcatttgatg ccgctcgcca gactcttgcg cgagcgcgga
      541 catcagaatc gtattggctt cttcctgcac gtcccctgcc cgccacctga catcctgacg
      601 gcgcttccga aacaccgcga aatcatcggt tctctggtcg ccttcgatct catcggtctt
      661 cagaccgaga acgactgcga gaacttgagc cgctaccttc tgcggatggg cgccaggcgc
      721 gcgcctgatg cgtatcacct ggaggcaaac gggcgcaggg ttacgttggg tgcattcccc
      781 gtcggcatcg aaacagcgcg cttcgaaaga acggcacgac gggccgcagc aaccgcgctg
      841 atcaaggaaa cggctgaaag tcttgccggc cgcgctatgg tgatgagcgt ggacaggctc
      901 gattactcca agggcctgcc aaaccgcatg gctgccattg agcgcttcct cagcatgtat
      961 cctgaatggc gcgggcgcgt cacattcctt cagattgcac ccaagacgcg ctctgagatt
     1021 caggaatatg cggaaatcga ccggcaggtc tccacgcttg cagggcagat caacggcctc
     1081 catggcgacg tcacgtggac ccccattcgc tatgtcaacc gggtctattc gcgggccgca
     1141 ctggccggcc tctaccggct ggcgaaggtt ggcctcgtga ccccgctcag agatggcatg
     1201 aatctcgtcg ccaaggagta cgtggcggcg caaaatccct tggacccggg cgtgctcgtg
     1261 ctttcgcaat ttgcaggtgc agccgcggag cttgatggcg cgctgatcgt caacccgcat
     1321 gagccggaag ctgtcgccgc tgcaatccgc ttcgcgctgg agatgccgct tgacgcaagg
     1381 caggcccgac atcagcggat gatggaggtc ctccggcgca acgatattca cgcctgggcg
     1441 tccaaatacc tcgccaccct cgagcagacc aggcccacgc cggcgaccat ttccgccacc
     1501 gcagcagccc gtcatctgcc gcccggctcg atcgcggcca gcgcttcccg ttagcaacag
     1561 tcgttaatcc tccgaggccg cggctccgcc cgcaaacgcg tcagaggttg catttcggcc
     1621 gtggacggca cacgttaacc caacccacgg caccaccgca ctctgatcgc ccatgtgtgc
     1681 acgctcgccg ttcacgttcg cccagcggct gattttctac gggctttcag tcgccgttcc
     1741 ggtgctcgcg atctgcgggt tgctcgtcta tcagtatact gcacagcaat accgggcgat
     1801 cgaggaggag acgctggcag cggctcagga gttggcgagg gatctcgaaa gagagatcca
     1861 ggtccagctt ttgctcctcg aggctttgat cggcgtgctc gggccagaga tggacttggc
     1921 ccgcctgcac cagaacgcaa gacgcgctct tgttccgcac ggcgcgcatg tcgtcgtcta
     1981 cgatgtcggc ggatggcaga ttctcaacac ctcggtgcca atgggcgacc cgctgccggt
     2041 gagctatgac ccggtccgaa taaaagccat cgtcgaacgt ggtcagccct atgtttcag
//
LOCUS       DASUMG010000287         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_37326, whole genome shotgun sequence.
ACCESSION   DASUMG010000287 DASUMG010000000
VERSION     DASUMG010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37326"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="VFX46_04750"
     CDS             complement(<1..114)
                     /locus_tag="VFX46_04750"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX5599921.1"
                     /translation="MASSELRTIHIRIEGRVQGVGFRAWVEDEARALGLDGW"
     gene            complement(280..356)
                     /locus_tag="VFX46_04755"
     tRNA            complement(280..356)
                     /locus_tag="VFX46_04755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Arg,seq:tct)
     gene            complement(404..709)
                     /locus_tag="VFX46_04760"
     CDS             complement(404..709)
                     /locus_tag="VFX46_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEX5599922.1"
                     /translation="MVARIYKPAKTAMQSGIARTKEWVLEFEPQLPRTIDPLMGWTGQ
                     QDPREQIQLTFDSKEEAIAYAERNGIAYILQDPEPRRPVLKSYADNFRYGRIGAWTH"
     gene            964..1278
                     /locus_tag="VFX46_04765"
     CDS             964..1278
                     /locus_tag="VFX46_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX5599923.1"
                     /translation="MIVEERDYRVKTGKLAEFVSLYEKHGLPIQLELLGTFLGYFTTE
                     FGELNHVVALWGYESLDDRQQRRDRMMADPRWQEYLKITQGLLEEQHSRILRPTSFSP
                     IK"
     gene            1301..2095
                     /locus_tag="VFX46_04770"
     CDS             1301..2095
                     /locus_tag="VFX46_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5599924.1"
                     /translation="MSSSFVVLPDGARLAVRTTGSGRDLVLVSGLGGTAAFWDPVVPH
                     LEPHFRVIRFDQRGIGRSDRGTDQVSINRLAHDTLAVLEHVDARNAMLLGHSTGGVIL
                     QELALIDPSRVGALVLSGTWARPNRFMAELFRSRTVLLKTVPREYAATLAFIGYTPEW
                     LDANWAHYEAMLAAAPMTSEQQKVVAERIEALLAFDRSSDIGSISLPTLIQGAEDDLI
                     VPAFLQRELASLMPKAELRILPSGGHFFPVTRLSEFVKMLLEWKNA"
     gene            complement(2138..>2286)
                     /locus_tag="VFX46_04775"
     CDS             complement(2138..>2286)
                     /locus_tag="VFX46_04775"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEX5599925.1"
                     /translation="ASLALRSDGIHKVSLDQVIATMRQTGADMQAKYKETSLGGLAVN
                     YTEC"
BASE COUNT          453 a          700 c          694 g          439 t
ORIGIN      
        1 ccagccatcg aggccgagtg ctctcgcttc atcttccacc cacgcccgaa agccgacccc
       61 ctgaacgcgg ccttcgatgc ggatatgtat ggtccggagc tccgatgaag ccatcagcac
      121 caccttttgc gcacaggggc attttactgt tccgcagcgg gcttaagatt ggatcagctc
      181 cgctgctttt gaacccgtcg aaaatctcct cgcccgggag ggtccactgc cgcagaatca
      241 gaatgacggc tgtccagaat cgtaagccat tgaaatagct ggcgaccccg gcaggactcg
      301 aacctgcgac cctctgctta gaaggcagat gctctatcca gctgagctac ggggccgtga
      361 ctcgtacaat acccgatcga agacgaggcg tcttgtcgaa tgcctagtgc gtccatgcgc
      421 caatgcggcc gtatctgaag ttgtccgcat aggatttcag aaccggcctg cgcggctctg
      481 ggtcttggag gatatacgca atgccgttgc gctcggcgta agcgatggcc tcctctttcg
      541 agtcgaacgt gagctggatc tgctcccgcg ggtcttgctg gccggtccac cccatcaatg
      601 ggtcgatcgt gcggggcaac tgaggctcaa actcaagcac ccactccttg gttcgggcaa
      661 tgcccgattg catggcggtc ttcgccggct tgtagatgcg agcaaccatg tgcggtcctg
      721 cagtcccaat cgaaaatctg tgcttgcgcc cggaacatac gcagtccgcg ccgacacttc
      781 aatgctgctt gatcgggatc ggcggccgcg gtgttgccgt gggaactaac acatgccaga
      841 tgaggacacc gacgccgttg gcggtctgag aagcatgccg gcaaccggct caatctgcct
      901 cgcagcgcgg atgacgccac cacgccgcag ccagcgacaa accaagattg tatggagaca
      961 aatatgatcg tcgaagaacg tgactaccgg gtgaagacgg gcaagctcgc ggagttcgtc
     1021 agcctgtacg agaaacacgg ccttccgatc cagctcgagc tgttgggaac cttcctcggc
     1081 tacttcacca ccgagttcgg cgagctcaac cacgtcgtcg cgctatgggg atacgaaagc
     1141 ctggatgacc ggcagcagcg ccgggatcgg atgatggctg atccgcgctg gcaggagtac
     1201 ctcaagatca cccaaggctt gctggaagag cagcactccc ggatactgag gccaacatcg
     1261 ttctcgccga taaagtagcg cagccacgga agccgatcat atgagcagct cgttcgtcgt
     1321 tctgcccgac ggcgctcgcc tcgccgtcag gaccacagga agcggccgcg atctcgttct
     1381 cgtcagcggc cttggaggaa ccgctgcgtt ctgggatccc gtcgtaccgc acttggagcc
     1441 gcacttccgg gtcatccggt tcgaccagcg cggcatcggc aggagtgacc gcggcaccga
     1501 ccaggtgtcc atcaatcggc tggcgcacga tacacttgca gtgctcgagc atgtcgatgc
     1561 ccgcaatgcc atgctgctgg gccattcgac aggcggggtc attctccagg aattggcgct
     1621 gatcgacccg tcgcgcgttg gtgcgctcgt gctcagcggc acatgggcgc gcccgaaccg
     1681 gttcatggcg gaactgttcc gttcgcgcac cgtcttactc aagacggtac cacgcgaata
     1741 tgccgccacg ctggccttca tcggctatac gccggagtgg ctggacgcca actgggcgca
     1801 ctatgaggcg atgctggcgg cagcaccgat gacgtccgag cagcagaagg tcgtggcgga
     1861 gcgcatcgag gcactgctcg ccttcgatcg aagcagcgac atcggatcga tcagcctgcc
     1921 gacgctcatt cagggcgctg aggacgatct tattgtgccg gcattcctcc agcgggagtt
     1981 ggcgagcctg atgccgaaag cagagctgcg cattctgccg agcggtgggc acttcttccc
     2041 ggtcacgcgg ttatcggaat tcgtgaagat gctgctggaa tggaagaacg cttagcctcc
     2101 acgaaagcgg ggccgcgagc ttcggcagac gcaggcttca gcactcggta taattcacag
     2161 ccaagccgcc gagcgatgtc tccttgtact tcgcctgcat atcggcgccg gtctggcgca
     2221 tggtagcaat aacttggtca aggctgacct tgtggatgcc gtcggagcgc agggctaagg
     2281 aagcgg
//
LOCUS       DASUMG010000288         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1081320, whole genome shotgun sequence.
ACCESSION   DASUMG010000288 DASUMG010000000
VERSION     DASUMG010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081320"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..>2333)
                     /locus_tag="VFX46_04780"
     CDS             complement(100..>2333)
                     /locus_tag="VFX46_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX5599926.1"
                     /translation="ITSFETRDDTHVLIRAPAFRGVLAASAFSLLATAWPAPAAGPQL
                     VAAPSAVPVVKANAARLPAKPLLTPDRAARESDHVARFDEAIAPAREHPVSEEDAKRI
                     ADAIGAYYARDESRGRALQREITDPIGRKLVEWYRLRAGLADAFQIRAFLEANPAWPD
                     RELLNRKFEELLFAQGGDAAAIKNAFSRQEPATGLGLAALASAYLVEGDTKRAKDLAS
                     RAWREFDIPAAYESGFLERFGSLLTEADHKWRFDRLLIDTPRWSSQRKERAAAAKRII
                     PLLSKAEQVKAEARLAVFLRQKSAAKLMAALPADSSADWGLAFQRADLLRRQGKDEEA
                     WTILLSAPTGREEAVNPDGWWTLRLAAAYDALRAGNPKMAFELVREAGPLSVNPHNQQ
                     TFFAGWLALRHLNEPQTALPFFEANEKSADGPLSRSQALYWLGRTHEALGKRSKAKEL
                     YKLAALYKDTFHAHLAREKLGKDAPKLSITPPEAPTAEQIDRFNNLDAVRAAVIARKA
                     GINNVLVRAFISELQRYFETEAEVAMVAHLAEALGDTQMALRVGKAAVARGMNLLYYS
                     YPIHAFPAYDPLRTPPEPALLLALARQESEFNGSIMSGAGARGILQVMPGTARHICRN
                     YRIKCNIKRLMNDPSYNAMLASAYVGDRMDDVSGSYILTLTSYNAGPGRTRQWLRELG
                     DPRDPSVDPIDWIHQIPFEETRDYVQKVLSNLQIYRARLGNYKGSIGADLVRARRKPD
                     AAP"
BASE COUNT          424 a          697 c          748 g          464 t
ORIGIN      
        1 catcaagcga ggtgaagaag atgtcccgcc agggctctgt ttcctttgcc ataacacgcc
       61 gtttttgttg ggaagctgaa gtcgataaat gctgagcgcc tacggtgcgg cgtccggttt
      121 ccgtctggca cggacgaggt ccgcgccaat ggagcctttg taattgccga gccgcgcccg
      181 gtagatttgc aggttcgaaa gcactttttg cacgtaatcc cgcgtttcct cgaatgggat
      241 ttggtgaatc cagtcgatcg ggtcgacgct gggatcgcgg gggtcgccga gctcgcgcag
      301 ccattggcgt gtgcgcccgg ggccggcatt gtagctcgtc agagtaagaa tatagctgcc
      361 cgacacatcg tccattcggt cgccgacata ggcactcgcc agcatggcgt tgtacgacgg
      421 atcgttcatc aagcgcttga tattgcactt gatccggtaa ttccggcaga tgtgccgcgc
      481 cgtgccgggc atgacctgca aaatgccgcg tgcgccagcg cccgacatga ttgagccgtt
      541 gaactcactc tcctggcggg cgagggcgag gagcagcgcc ggctcagggg gtgtgcggag
      601 cgggtcatag gccgggaaag catgaatggg ataagagtag taaagaaggt tcatgccgcg
      661 cgcgacagcg gctttgccca cgcgcaacgc catctgtgtg tcgccgagtg cttcagcgag
      721 atgggcgacc atcgccacct ccgcctcggt ctcgaaatat cgctgcagct ccgaaatgaa
      781 ggcgcgcacc aacacgttgt tgatcccggc ttttctagcg atcacagcgg cacggactgc
      841 gtcgaggttg ttgaagcggt cgatctgctc ggcggtcggt gcctcgggtg gggtgatcga
      901 gagcttcggc gcgtctttcc ccaacttttc gcgggcaaga tgggcgtgga acgtgtcctt
      961 gtagagagcc gcaagcttgt acagctcttt ggctttgctc cgcttaccga gcgcctcatg
     1021 ggtgcgtccc aaccaataga gggcctgtga ccggctcaaa ggcccatcgg cgctcttttc
     1081 gttcgcttca aaaaacggca aggccgtttg cggttcgttc aagtgccgga gagccagcca
     1141 tccggcaaaa aaggtttgct ggttgtgcgg attgacgctg agaggaccgg cctcgcggac
     1201 aagctcgaag gccatcttgg ggttaccggc ccgcaaggcg tcgtaggcag ccgccagcct
     1261 gagtgtccac cagccgtcag ggttgaccgc ctcttcgcga ccggtgggcg cagagagcag
     1321 tatggtccag gcctcttcgt ccttgccttg tcggcggagc aggtcggccc tctggaacgc
     1381 aagcccccag tccgccgagc tatctgccgg cagggctgcc atcagcttgg ccgccgattt
     1441 ctggcgcagg aacactgcaa gccgcgcctc ggctttcacc tgctcggcct tgctcagcag
     1501 gggaatgatg cgcttggcag ctgcagcccg ctccttccgt tggcttgacc atcgaggagt
     1561 gtcgatcagt agccgatcaa accgccactt gtggtcggct tctgtcagaa gactgccaaa
     1621 tctttcgagg aagccacttt cgtacgctgc tggaatatcg aactctcgcc aagcacgcga
     1681 cgcgagatct ttcgcgcgct tggtgtcacc ctcgacgaga taggcggaag caagcgctgc
     1741 cagtccaagg cccgtggctg gctcttgcct cgaaaaggcg ttcttgatcg cagctgcgtc
     1801 tccaccttgc gcaaaaagca gctcctcgaa tttccggttg aggagttcgc gatccggcca
     1861 ggcggggttg gcctcgagga acgcccggat ctggaaggca tccgccaacc cggcgcgcag
     1921 ccgataccac tcgacgagct tgcggccgat cgggtcagta atctctctct gcagtgcccg
     1981 tccgcgcgac tcgtccctgg cgtagtaagc gccgatcgca tcggctatgc gtttcgcgtc
     2041 ctcctcgctg acgggatgct cacgggcagg cgcaatcgct tcgtcgaaac gggcgacgtg
     2101 atcggattca cgtgcggcgc gatccggcgt cagcaacggc ttggcaggaa ggcgcgcagc
     2161 gttcgccttg acgacaggaa ccgcggatgg cgccgcaacg agctgcggac cagctgcagg
     2221 agcaggccac gcagtggcca gaagcgagaa ggcggatgcc gcgaggacac ctctgaaggc
     2281 gggagcgcga atcagcacgt gcgtatcgtc tctcgtctcg aagctggtga tcg
//
LOCUS       DASUMG010000289         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_599163, whole genome shotgun sequence.
ACCESSION   DASUMG010000289 DASUMG010000000
VERSION     DASUMG010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599163"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VFX46_04785"
     CDS             complement(<1..962)
                     /locus_tag="VFX46_04785"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX5599927.1"
                     /translation="MKPIHEIGEKLGIPPTALLQYGPTKAKVSYDFIKSIQDRPDGKL
                     ILVTAINPTPAGEGKTTTTVGLGDGLNRIGKKAMCCLREPSLGPCFGMKGGAAGGGYA
                     QVVPMEDINLHFTGDFHAITSANNLLAAMIDNHIYWGNELGIDPRRVAWRRVMDMNDR
                     ALRSIVNSLGGVANGFPRQDGFDITVASEVMAILCLAMDLDDLQRRLGNIVVGYTRDK
                     KPIRARDVKGDGAMTVLLKDALMPNMVQTLENNPVFIHGGPFANIAHGCNSVLATRTA
                     LKLADYVVTEAGFGADLGAEKFFDIKCRAAGLSPDAAVLVATIRA"
     gene            complement(1155..1967)
                     /locus_tag="VFX46_04790"
                     /pseudo
     CDS             complement(1155..1967)
                     /locus_tag="VFX46_04790"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
     gene            complement(2317..3588)
                     /gene="frc"
                     /locus_tag="VFX46_04795"
     CDS             complement(2317..3588)
                     /gene="frc"
                     /locus_tag="VFX46_04795"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX5599928.1"
                     /translation="MKALEGVRILDFTHVQSGPTCTQLLAWFGADVIKVERPGVGDIT
                     RGQLRDVPNADSLYFTMLNHNKRSITLDTKHPKGKEVLERLIKTCDVLVENFAPGVLD
                     RMGFSWERIQELNPRMIYASIKGFGPGPYEDCKVYENVAQCTGGAASTTGFDDGPPLV
                     TGAQIGDSGTGLHLALGIVTALYQRTHSGRGQRVTCAMQDGVLNLCRVKLRDQQRLAH
                     GPLTEYPQYSEGKPFGDTVPRSGNASGGGQPGWILKCKGWETDPNAYIYFIAQAPVWD
                     KICDVIGEPDWKTHPDYATPQARLPRLKQIFDRIEQWTMTKTKFGAMDILNKYDIPCG
                     PILSMKELAEDQSLRATGTIVEVDHPTRGKYLTVGNPIKLSDSPAEVKRSPLLGEHTE
                     EILRDVLGYSEAEIAELREATEPAPKRAAAE"
     gene            complement(3599..>3879)
                     /locus_tag="VFX46_04800"
     CDS             complement(3599..>3879)
                     /locus_tag="VFX46_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX5599929.1"
                     /translation="NNGIYRGVEVNPSGGPDPAITVFVKDARYDKMMEAFGGVGAYAT
                     TPDELKRAVDAALDSGKPTLINAVIDPAAGSESGRIGNLNPQSVLKKK"
BASE COUNT          697 a         1157 c         1192 g          833 t
ORIGIN      
        1 gcgcggatcg tcgccacgag caccgcggcg tcgggcgaga ggcccgccgc gcggcacttg
       61 atgtcgaaga acttctcggc gccgagatcc gcgccgaagc cggcctcggt caccacgtaa
      121 tcggcgagct tcagcgctgt ccgcgttgcc agcaccgagt tgcagccatg cgcaatgttg
      181 gcgaacggac cgccgtggat gaagacgggg ttgttctcga gcgtctgcac catgttgggc
      241 atcagcgcgt ccttgaggag gacggtcatg gcgccgtcgc ccttcacgtc gcgggcgcgg
      301 atcggcttct tgtcgcgcgt gtagccgacc acgatattcc cgagccgccg ctgcaggtcg
      361 tcgaggtcca tggcgaggca aaggatggcc atgacctcgg aggcgacggt gatgtcgaag
      421 ccgtcctgcc gcgggaagcc gttcgctaca ccacccagcg agttgacgat ggagcgcagc
      481 gcgcggtcgt tcatgtccat gacccgccgc caggctacgc gccgcggatc gatgccgagc
      541 tcgttacccc agtagatgtg attgtcgatc atcgcagcga gcaggttgtt cgccgacgtg
      601 atcgcatgga aatcaccggt gaagtggagg ttgatgtcct ccataggcac gacttgggcg
      661 taaccgccgc cggccgcacc gcccttcatg ccgaagcatg ggccaagcga aggctcgcgc
      721 aggcagcaca tggccttctt gccgatccga ttgagaccat ccccgaggcc aacggtcgtg
      781 gtggtcttgc cctcaccggc gggcgtcggg ttgatcgcag tgacaaggat cagcttgccg
      841 tccggccggt cctgaattga cttgatgaag tcgtaggaga cctttgcctt agtcggcccg
      901 tactgaagca gggcggtggg tggaataccc agcttctcgc cgatctcatg gatcggcttc
      961 attttcgcct cacgggcaat ctcgatatcg gaccttacgg ccacggatgt tcctcctgct
     1021 cccttctttg attccgccgc cggcttcggc gaaggtggat cgaaccgatc tccgaccttg
     1081 agcccggctg ataccgcgta ctcagtggct ggaattttct gcccgcccgc tgggcgaaca
     1141 cgcttgatca gaatgcggcc acccttggct gcaacctgaa tgccctcccc ggtgatcgcc
     1201 atgatctcac cggggttgcc agaaccatcg agcttcgccg aatcgaagat gtcgagccga
     1261 gcacccttga tcgtgctcca ggctccgggg gctgggttgg tcgcgcggat gaggttgtag
     1321 acctcagaaa ctggcttcga ccaatcgatt tcggccagat cttttttgaa ccagctctcg
     1381 tatgtggcct tcgaatggtc ttgcggaatc ttcggtgctt tgccggcccg tacgaggtcg
     1441 acggcctcca tcatggcgtc gacgccgagc gggaagagct tattgaaata aacgtcgccg
     1501 agtgtctcgt ctggaccgat ctcgacctcc ttctgaagga ggatggggcc ctcgtcgagt
     1561 ccttcatcgg gccagaaaat cgtcaacccc gtgcgcgtct tgcccatggc aatcggccag
     1621 ttgatggacg aggggccgcg gtgcaggggc agaagtgagg gatggtattg gatcgtcccg
     1681 agtttcggcg catcgagcac cggctgcggt acgaacagcg tgacgtaagc catcacgcat
     1741 aggtccggct cgtaagagcg catcaactcg agtgcttctg gcgtcttcca ggatttgggc
     1801 tggtggaccg gcaggccttt ttcctcggca aagacggcga gcggatcacg tgccttgcct
     1861 tccttgtctg gagcgcagaa cacggcgaca acattctcgc cgcgctcaag aagcctctcc
     1921 aggaccgcct taccgaaagc ttgctgcccg tgaactacga tgcgcatgtg gatccttccc
     1981 aattatgtac tttgcttatc tggactgccc cttatccgtc attcccgcca ctgcgaaaat
     2041 tcaggccacc ttttctactg gatgccgctg gcggtcgaac taggcatgaa ctccgtgtcg
     2101 tttcttgcct tcgatcgggt cgtatttgta ccgagataga tgatttagct ctaatctgtc
     2161 acgtcggcac agactgcgcc attgaattgg tgcgagtgta agtgtgggga aatccagacc
     2221 atgcagtgat gcagattagg tgcactcgaa cgacgccggg aggggattcc ccgcctcccg
     2281 attggcttgc taggatggga ggcggggttc aggatattac tccgcggcag cccgcttcgg
     2341 agctggctcc gtagcttccc tcagctcagc gatttccgcc tcactgtagc cgagcacatc
     2401 gcggagaatt tcctccgtgt gctcgccgag caggggtgag cgcttcactt cggccgggct
     2461 gtctgaaagc ttgataggat tgccgacggt caggtacttg ccacgagtcg gatggtccac
     2521 ctcgacgatg gtgcctgtgg cccgcagcga ctgatcttct gccagttcct tcatcgacag
     2581 gatgggcccg caggggatgt cgtacttgtt gaggatatcc atggccccga acttcgtctt
     2641 ggtcatggtc cactgctcaa tgcgatcgaa gatctgcttg agccgtggga ggcgcgcctg
     2701 tggcgttgca tagtccgggt gggtcttcca atcgggctcg ccaatcacat cgcaaatctt
     2761 gtcccagacg ggcgcctgag cgatgaagta gatgtaggcg ttgggatcgg tctcccagcc
     2821 cttgcacttc aaaatccagc cgggctggcc tccgcccgag gcgttgcctg agcgtggcac
     2881 ggtatcgccg aacggcttgc cttcgctgta ctgcggatac tcggtcagcg gaccgtgggc
     2941 gagacgctgc tgatcgcgca gcttcacgcg gcagagattg agcacgccgt cctgcatggc
     3001 gcatgtgacg cgctggcctc ggcccgagtg cgtacgctga tagagcgcgg tcacgatgcc
     3061 cagtgccaga tgcaggccag tgccgctgtc gccgatttgt gcgccggtga cgagcggagg
     3121 accgtcgtca aagccagttg tggatgcggc gccaccggtg cactgagcga cgttctcgta
     3181 gaccttgcaa tcttcatagg ggccaggacc aaaacccttg atagaggcat aaatcatccg
     3241 cgggttaagc tcctggatgc gttcccagga gaagcccatg cggtcgagca cgcccggcgc
     3301 gaagttctcc accagcacgt cgcaggtctt gatcaagcgc tccagcacct ccttgccctt
     3361 gggatgcttc gtatcgagcg tgatggaacg cttgttatga ttgagcatcg taaaatagag
     3421 gctgtccgca ttcgggacat cccgaagctg gccgcgggtg atatcgccga cgcccggccg
     3481 ctcgaccttg atgacgtcgg caccgaacca agcgagaagc tgcgtgcagg tcggaccaga
     3541 ctgcacgtgc gtgaaatcga gaatacggac gccttccagg gctttcattt caagctccct
     3601 atttcttttt cagcacgctt tgcggattga gatttccgat acggccgctt tcgctgcctg
     3661 ccgccgggtc gatgactgcg ttgatgagtg ttggcttgcc cgagtccaga gctgcatcta
     3721 ctgcacgctt cagctcatcg ggcgttgttg cgtaagcccc aacgccgcca aaggcttcca
     3781 tcattttgtc gtagcgggcg tctttgacga aaacggtgat cgccggatcg ggtccaccag
     3841 agggattgac ctcaaccccg cgataaatgc cgttgttgt
//
LOCUS       DASUMG010000290         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_840405, whole genome shotgun sequence.
ACCESSION   DASUMG010000290 DASUMG010000000
VERSION     DASUMG010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840405"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1423
                     /gene="pyk"
                     /locus_tag="VFX46_04805"
     CDS             2..1423
                     /gene="pyk"
                     /locus_tag="VFX46_04805"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX5599930.1"
                     /translation="MVATLGPASSDPDMLERLFAAGVDVFRINMSHSTHEVARKLHAA
                     VRNAEVKFRRPIGILCDLQGPKFRIGEIEGGRTFLSEGSLFRFDTNEEPGTSERVFLP
                     HPQIFEAVEPGHTLLLDDGKLKMRVVDRSVREITAEVLVGGALASRKGIATPDSILPI
                     NPITEKDRADLDYALRLGVDWVALSFVQRGEDLVLARRLIGQAASIMAKIEKPSAIAD
                     LDDIIANADGFMVARGDLGVEMPIEKVPGLQKEITRKARAAGKPVVVATQMLESMISS
                     PVPTRAEVSDVATAVFDGADAIMLSAESAVGQFPLEAVQMMNRIAIQVENDPGYDDFV
                     HATATVPQPTGADAIAAAVHKIARTLKLSAIICYTATGSTALRVARERPGLPVIGLTP
                     VLATGRKLSLVWGIHSVLTSDPANLADMVRKACRIAFEEKFAAAGDGVIITAGVPLGT
                     PGTTNMVRMAYLDENGAPLADQG"
     gene            complement(1506..>2087)
                     /locus_tag="VFX46_04810"
     CDS             complement(1506..>2087)
                     /locus_tag="VFX46_04810"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5599931.1"
                     /translation="ELELPDLSPPEMEGEGDEALPELREKLPDSAVERAKMLDNLYAL
                     LATAEDEHAADKTSKAIMRLWAVSGSDTVTVLMARAIKAVKEKNTKLALRMLDEVVEQ
                     APDYAEGWSQRGHLLYLEGETHRALGDLRRALALDPNHFRALGALAHILREYGANESA
                     LEAYRKLLQIHPFADGAQKAVDELEREVRGQGI"
BASE COUNT          382 a          649 c          654 g          402 t
ORIGIN      
        1 aatcgtcgcg acgctcggcc ccgcctcgtc cgacccggac atgctcgagc gcctgtttgc
       61 ggcgggcgtt gatgttttcc gcatcaacat gagccattcg acccacgagg tcgcgcggaa
      121 gctccatgcg gcggttcgaa atgccgaggt gaagttcagg cggccgatcg gaattctctg
      181 cgacctgcaa ggcccgaagt tccggattgg cgagatcgag ggcgggcgaa cgtttctcag
      241 cgagggctcg ctgttccggt tcgacaccaa cgaagagccc gggacgtcgg agcgggtctt
      301 tttgccccat ccgcagattt tcgaggcggt cgagccaggc cacacgctct tgctggacga
      361 cgggaagctc aagatgcgcg tggtcgaccg tagcgtgcgg gagatcacgg ccgaggtctt
      421 ggtgggcgga gcattggcaa gccgcaaggg catcgccacg ccggacagca tcctgcccat
      481 caacccgatc accgagaagg atcgcgccga tctcgattat gccctgcgcc taggcgtcga
      541 ttgggtggcg ctttctttcg tccagcgggg tgaggatcta gtgcttgccc gccgcctgat
      601 tggccaggcg gcctcgatca tggccaagat cgagaaacca tcggccatcg cggatctcga
      661 tgacatcatc gcgaacgccg acgggttcat ggttgcccgt ggcgacctcg gcgtcgagat
      721 gcccatcgag aaggtgccag ggctccaaaa ggaaatcacg cgaaaggccc gcgccgcagg
      781 caagccggtc gtcgtggcga ctcagatgct ggagagcatg atctcttcgc ccgtgcccac
      841 ccgcgctgag gtttccgacg tcgcaaccgc tgttttcgac ggcgcggatg ccatcatgct
      901 gtcagcagaa tccgctgtcg gacagttccc cttggaagcg gtgcagatga tgaaccgcat
      961 cgccattcag gtggagaacg atcccggcta cgatgacttt gtgcatgcta ccgcaacggt
     1021 tccccagcca acgggcgctg atgcgatcgc tgccgccgtt cacaagatcg caaggacgct
     1081 gaagctctct gccatcatct gctacacggc gactggctcg acagcgctcc gggtggctcg
     1141 cgagcggccg ggtttgccgg tcataggcct tacacccgtg ctggcgacgg gacgcaagct
     1201 atcgctggtg tggggtattc attcggtgct gacgagcgac cccgccaacc tcgcggacat
     1261 ggttcgcaag gcttgccgaa ttgcctttga ggagaaattc gcagcggcgg gcgatggggt
     1321 catcatcaca gccggtgtcc ccctcggcac acctgggacc acgaacatgg tgcgcatggc
     1381 gtacctcgac gagaacgggg cgccattagc tgaccaaggg tgaattggtg ccgaggcgcc
     1441 gattacattc gaagtctctt taaggagctc gttgaagccg gccacgaata gcaggctggc
     1501 agccgtcaga tgccctggcc gcgcacctcc cgctcaagct cgtcgacggc cttttgcgca
     1561 ccatcggcaa acgggtggat ctgaagcagc ttgcgatacg cctcgagcgc cgactcgttc
     1621 gcgccgtatt cgcgcagaat atgtgccagg gcacccaagg cccggaaatg gttcggatcg
     1681 agcgccagag cgcgccggag gtctcccagc gcacgatggg tttcgccctc caggtaaagc
     1741 aagtgaccac gctggctcca gccttctgca taatcgggtg cctgctcgac cacctcatcg
     1801 agcattcgga gagctagctt ggtgttcttt tccttaacgg ccttgatcgc tcgggccatg
     1861 agcacggtaa ccgtgtcgct gcccgagaca gcccacaacc tcataatggc cttggaggtc
     1921 ttgtcggccg cgtgctcgtc ctctgcagtc gccaacaagg cgtagaggtt gtcgagcatt
     1981 ttggctcgtt cgactgcact gtcgggcagt ttctcgcgca gctcgggcag agcttcatcc
     2041 ccctcgcctt ccatctcagg aggcgacagg tcgggtaact ctagctc
//
LOCUS       DASUMG010000291         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_198712, whole genome shotgun sequence.
ACCESSION   DASUMG010000291 DASUMG010000000
VERSION     DASUMG010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198712"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1547
                     /locus_tag="VFX46_04815"
     CDS             96..1547
                     /locus_tag="VFX46_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX5599932.1"
                     /translation="MSTATLVKAVPQGDGPKPLVENSAAVSEPSHALARFAANLRHQD
                     IPEEVRSRARHHALDAIGIALASTKFDFAHRTLNAVAGLGGAGPVPVIGMPARLSPRD
                     AAIVNGLLCHGLDFDDTHLGGVVHPTASAFPAALSAAMHAGASGRDLLTAYIIGVEAT
                     ARLGAVVKGAFHQVGFHPTGLVGAFGCALTAGVLFGLSERELVQAQGIALSLASGSLE
                     FLEDGAWNKRIHPGWAAAAGITAAALAKQGFRGASRPYVGRFGLYKSHLGPLAEGCDY
                     TLATTGLGETWEIARTSIKPFPACHFTHGCIDAALRLKKDGLDISAIEKIEALVPAEV
                     VKTVCEPEANKKRPANSYDAQFSIPFLVAAALVRGRITLAEIEDAALSDPQILAIADK
                     VQYRIDPNSPFPKAYSGEVRIALKDGEQVAAREEINRGAPDRPLSESEIVEKYRANAA
                     MSVSPAVADRIENAVLNLDEAQDLGNFAAAIAG"
     gene            1589..>2091
                     /locus_tag="VFX46_04820"
     CDS             1589..>2091
                     /locus_tag="VFX46_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5599933.1"
                     /translation="MSASNPKIVDDIAENERIILESVDRFLDRHVRPHVMRLEHDDIY
                     PDEIVERMKELGLFGAIIPTEYGGLGLSATTYAKIIERISSAWMSISGIINSHLIMAH
                     IVAKMGTEEQRKEFLPRFATGELRGGLALTEPDCGTDLQAIRTTAVRDGDHYVVNGTK
                     TWISNGI"
BASE COUNT          409 a          682 c          635 g          365 t
ORIGIN      
        1 acctgtcgga tggccaaaaa cgtccgatag acgagagctg cgccaaagaa gcgcgtcact
       61 ctcgatccgc aacgagaagt tcaagggagg agcgcatgag caccgcgacc ctagtgaaag
      121 ctgtccctca gggcgacggc ccgaagcctc tcgtcgaaaa ttcggccgcc gtcagcgagc
      181 cttcccacgc gcttgcgcgt ttcgccgcca atttgcgtca tcaggatatt cccgaagaag
      241 tccggtcgcg ggcgcgtcac cacgcgctcg acgcaattgg aatcgccctc gcgtccacca
      301 agttcgactt cgcccaccgg acgctgaacg ctgtcgcggg actgggcggc gcggggccgg
      361 ttccggtaat tggcatgccc gcccggctct ccccgcgcga tgccgctatc gtgaatgggc
      421 ttctctgcca cggcctcgac ttcgacgata ctcatctcgg cggagtcgtc catccgacag
      481 cgagcgcgtt tccggccgct ctttcggcgg cgatgcacgc tggcgcaagt ggccgggatc
      541 tccttacggc atacatcatc ggcgtcgagg ccacggcccg cctcggcgcg gtcgtgaagg
      601 gcgccttcca tcaggtgggc ttccacccga ccggccttgt cggagctttt ggctgtgcgc
      661 tcacggccgg cgttttgttt ggcctctcag agcgcgagct cgtgcaggca caaggcatcg
      721 ctctgtccct cgcctccggc agccttgaat tcctggagga cggtgcgtgg aacaagcgaa
      781 tccatcccgg ctgggccgct gccgcgggca ttactgcggc cgccctcgcc aaacagggct
      841 tcaggggtgc gagccgtccg tatgtcggac gtttcgggct ctacaaatcg cacctgggac
      901 cgctcgcgga aggttgcgac tacacgcttg cgaccaccgg cctcggcgaa acttgggaaa
      961 tcgcccgcac ctcgatcaaa ccgtttccgg cgtgccattt cacccacggc tgtatcgacg
     1021 ccgccctgag actgaagaag gacgggctcg acatttcagc aatcgagaaa atcgaggctc
     1081 ttgtccctgc cgaggtggtg aagacggtct gcgagcccga ggcgaacaag aagcggccgg
     1141 caaacagcta cgacgcgcag ttctcgatcc cgttcctcgt cgcagctgcc ctggtgcgcg
     1201 gccgcataac tcttgccgag atcgaggacg cggcgctttc cgatccccag attctcgcga
     1261 tcgccgacaa ggtccagtat cggatcgatc ccaattcgcc gttcccgaag gcctactccg
     1321 gcgaggtgcg gatcgctttg aaggacggcg agcaagtcgc cgcgcgcgaa gagatcaacc
     1381 gcggcgcgcc ggaccgtccg ctgagcgagt cagaaattgt cgagaaatat cgcgcaaatg
     1441 ccgcgatgtc ggtgagtccg gcggtggctg accgcatcga gaacgccgtg ctgaacctcg
     1501 acgaagcgca ggatctcggc aacttcgcgg ccgccatcgc gggctaagcg ccaaagcgaa
     1561 atcaacatcg aaattcgaag gatccggaat gagcgcttcc aacccgaaga tcgtcgacga
     1621 catcgcggaa aacgaacgga tcattctcga gagtgtcgat cgtttcctcg atcgtcacgt
     1681 gcgtccgcac gtcatgcggc tcgagcacga cgacatctat cccgacgaga tcgtcgaacg
     1741 aatgaaggag ctcgggctgt tcggcgcgat catccccacc gagtacggcg gtctcggtct
     1801 cagcgctacc acttacgcca agatcatcga acgaatctcg agcgcgtgga tgtcgatcag
     1861 cggcatcatc aactcgcacc tcatcatggc gcacatcgtc gcgaagatgg gcacggaaga
     1921 gcagcgcaag gagtttctgc cgcgtttcgc gaccggggag ttgcgcggcg gccttgcact
     1981 caccgagcca gattgcggca cggacctcca ggcgatccgc acaacggccg tccgagatgg
     2041 tgaccactac gtcgtcaacg gcaccaagac gtggatctcg aacggcatca a
//
LOCUS       DASUMG010000292         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_359160, whole genome shotgun sequence.
ACCESSION   DASUMG010000292 DASUMG010000000
VERSION     DASUMG010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359160"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1373
                     /locus_tag="VFX46_04825"
     CDS             87..1373
                     /locus_tag="VFX46_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX5599934.1"
                     /translation="MTIFLVFAGLLALLLSGVPIFAALGLTATVILLLFEGSVSSLAE
                     TTYSHLANPVLTTIPLFVFMAQVMIRAKVIDDLYETAHTLVGHIRGGLGIATVMACTV
                     FASISGSSVATALSIGSTAIPQMKRFGHSERSALGVVAAGGTLGILIPPSGPMILYAI
                     VSEASIGALFLAGIIPGLLLAALFSLYCLIEAALAGRADKPAWAGWPTVWSAVRRSIW
                     ALMAPPVVMGGIYFGVFTATEAAAAGAIYALLVAVLVYRNFGLADLWACAYSTMRTTM
                     MVFMIIAAAAMFGYAITIIRLPVGVTEAVAQMDLGPLGFIIAVMALIFVLGMFLESIA
                     IILITTPILLPTLHTLGIDPIWYGVLLMVNLELAMITPPVGMNLFAIKGITGAPLSEI
                     VKGAAPYVAIMIIGMAILIAFPELATWLPFRAGFGR"
     gene            1557..1835
                     /locus_tag="VFX46_04830"
     CDS             1557..1835
                     /locus_tag="VFX46_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599935.1"
                     /translation="MAKRRRDPVRPTQAHEINEVDLAQDMMGRNALQGDDQANVHNQR
                     YVEPGVNREPDDLIETFEKADKDVRARRDLGKGRRHSPKHPYNQPPEE"
     gene            1961..2128
                     /locus_tag="VFX46_04835"
     CDS             1961..2128
                     /locus_tag="VFX46_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599936.1"
                     /translation="MNSRTLILAAVLVIVAIAGYLYMMPEVEVMRSEPATTSEPATAS
                     EPTITTPSGSQ"
BASE COUNT          434 a          738 c          742 g          535 t
ORIGIN      
        1 atggcgccgc tgctcgccgc ctgcgttctc cttgcgctga tggcggcagc ccgcctgctg
       61 gatgcattag cgcgaggagc gcagcgttga cgatcttcct cgtctttgca gggctgcttg
      121 cgctgttgct ctcgggcgtt cctattttcg cggcgctggg actgacggcg acggtcatcc
      181 tgttgctctt tgagggcagt gtctcgagcc ttgccgagac gacctacagc catctcgcca
      241 accccgttct gacgacgatt ccgctgttcg tcttcatggc gcaggtcatg atccgcgcca
      301 aggtgatcga cgacctttac gagacagcgc acacgctcgt cggtcatatc cgcggcggcc
      361 tcgggatcgc caccgtcatg gcctgcacgg tgttcgcctc gatctccggc tcttcagttg
      421 cgacggcgct ttccatcggc tcaaccgcca ttccgcagat gaaacggttc ggtcattcgg
      481 agcggagcgc attgggtgtc gttgccgccg gtgggacgct tggcattctc attccgccgt
      541 ccgggccaat gatcctatat gccatcgtct ctgaagcttc gatcggcgct cttttcctcg
      601 ccggcatcat cccggggttg ctgctggctg cgctgttctc gctttactgt ctcattgagg
      661 cggccttggc cgggcgtgca gacaagccgg cctgggcagg atggcccacc gtctggtcgg
      721 ctgtccggag gtcgatctgg gcgctcatgg ctccgcctgt ggtgatgggt ggcatctact
      781 tcggcgtctt taccgcaacc gaggccgccg ccgcgggcgc gatctacgcg ctcctggttg
      841 cggtgctcgt gtaccgcaac ttcggattgg ccgacctgtg ggcgtgtgcc tactccacca
      901 tgcgcacgac catgatggtg ttcatgatca tcgctgccgc cgcgatgttc ggttacgcaa
      961 tcaccatcat ccggttgcct gtgggtgtga ccgaagcggt tgcgcagatg gatctcgggc
     1021 cgctgggctt catcatcgcc gtcatggcac tgattttcgt gctgggcatg ttcctcgagt
     1081 ccatcgcgat catcctcatc acgacgccaa ttctattgcc gacgctgcat acgctcggga
     1141 tcgatccgat ctggtatggc gtgttgttga tggtcaatct cgagctggcg atgatcaccc
     1201 ctccagtcgg catgaacctc ttcgcaatca aggggatcac cggcgcgccg ctctcagaga
     1261 tcgtgaaagg cgcggcgcct tacgtagcca tcatgattat tggcatggcc attctcatcg
     1321 catttccaga gctggcgacc tggctgccgt ttcgcgccgg cttcgggcgc taaacacagc
     1381 cttgcgggct tgccagccca ttgacttcgt tgagcgtcct ctttgcgagg gaacaaatcg
     1441 ctaacttagc tgtcggagta tggctcgcgg ccggacgcct cattgaggaa ccgcaacgtc
     1501 ggcctcgtgt tggaggatta cgaagtccga agactggagt tgggaggtac ggtgagatgg
     1561 cgaagaggcg aagggaccct gtgcggccga ctcaggctca cgagatcaat gaggtcgatc
     1621 ttgcccagga catgatggga aggaatgccc tccaaggcga tgaccaggcg aacgttcaca
     1681 accagcgtta tgtcgagcca ggagtgaacc gggagcccga cgatctgatc gaaaccttcg
     1741 agaaggccga caaagacgtt cgtgcgcgac gggatctcgg caaagggcgg cgccactcac
     1801 cgaagcatcc ctacaaccag ccccccgagg aatgaacgct cattctcgcg ccattttggc
     1861 aacagttttg aggcgaacgc gccgttcata ggagcgtccc cttctcaaca tattggaaga
     1921 atatcgtgtc agccttcgga tcaactctgg aggatgcacg atgaatagtc gaacgctgat
     1981 tttagcggcg gtgctcgtca tcgtggcaat tgctggatac ctctacatga tgccggaagt
     2041 ggaagtaatg cggtcagagc cggcgacgac cagcgaaccg gcaacggcta gcgagccgac
     2101 gattacgacc ccgagtggct cgcagtaacg atttccagga tattgtcgac cggcatcggg
     2161 aatcgcagct cgaggctgcc tggtgccggt tgactagttt ggctgccacg ggaccataac
     2221 gacacgccat ccggtgcgtg gcttgcaggg agctcaacaa agacgtccgt tcgtgccggg
     2281 gaggtgcgat cagagcgacg tgctcgcaac tgccacccgc cataacgggt cccgctgcca
     2341 ctgcggcagg tcgccggtgg agttctgcaa ttcgagcagg cgctttcccc catcgacata
     2401 cttgattcgg tgctcattct gtcccacaag cagcacccat cgaggcaag
//
LOCUS       DASUMG010000293         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_198874, whole genome shotgun sequence.
ACCESSION   DASUMG010000293 DASUMG010000000
VERSION     DASUMG010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198874"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1237
                     /locus_tag="VFX46_04840"
     CDS             221..1237
                     /locus_tag="VFX46_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX5599937.1"
                     /translation="MTEPIPVAVVGAGYFGRLHAKHYAANPAAKLVAVVDIDPAKARA
                     VAEEFGAEAFCDYRSVIGRVIGASVAVPTALHFEVASALIDNGIHVLVEKPITDRLET
                     ALALASLAEERRRVLQVGHIERFSSTYRALAREVTRPLYIESYRVSPWKQRSGEVDVV
                     LDLMIHDIDLIQGLVGLPATMVHAVGMPVINATTDLANARIVFGSACVATITASRISH
                     KTERRIRVFQPNRYIIGDFANHRIERYSVCGNPARIGLAAIKFDSVEVPKEDSLANEI
                     AEFLECISTGRRPTVTGREACEAMRLAELISDSIDGHRLMANGALRNDAQWDEGSAPY
                     GASA"
     gene            1234..>2360
                     /locus_tag="VFX46_04845"
     CDS             1234..>2360
                     /locus_tag="VFX46_04845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5599938.1"
                     /translation="MKHAFALRDTNPAPARVKVPLFDLSRQAERFGPKLNQRIAEVLQ
                     HGRFILGPEVEELEAALARFTGARHVIGVSSGRDALIISLMALGVGAGDAVFVPAFTF
                     SATAGAVVAAGAVPVFTDVDPATLTMDAEQLERAIKRTLRNTALRPRAVIPVDLYGLP
                     ADYAAIEAVATRYGLSIIADAAQSFGARVGSRRVGTLAEITATSFYPTKPLGAFGDGG
                     AIFTDDDQLAQAVREIRSHGTRGSGDVAMRLGLTGRLDTLQAAVLLVGLEVFEERLAS
                     RREIAARYQRAFSGVVGTPPQSALLCSAWALYTIRTRDRDNVRGRLAAAGVETGLYYR
                     VPLHRHPAFAGWLTGEEELPVSEQAADEVLSLPLHAGLSEE"
BASE COUNT          427 a          704 c          752 g          477 t
ORIGIN      
        1 gtcccaggta aatgcgatcc gtgtgctatt caagcggagc tgcgtcgttg aaggagatcg
       61 acgtcggtat gtgctgtcgc gtgttccgcc accttgccat cggtgtccac agacaaaagt
      121 ggataaccgc tcatttcccg cgctggttgg cccccgcgcc acttctcatc cataggtggt
      181 gtgcgtaaca ttcctgccac ttttggttcg ggtggggaac atgacagagc caataccggt
      241 cgctgtcgtc ggggcgggat acttcggacg cctccatgcg aaacactacg cggccaatcc
      301 ggcagccaag ctggtcgctg tggtcgacat cgatcccgca aaggccaggg ccgtggcgga
      361 ggagttcggg gccgaggcgt tttgcgacta ccgctccgtg atcggccgtg tgattggcgc
      421 gagtgtcgcc gtgccgacgg cgctgcattt cgaggtcgcc agcgctctga tcgacaacgg
      481 cattcacgtt ttggtcgaga agccgattac cgacaggctg gagactgcgc tggcactcgc
      541 gtctcttgcg gaggaacggc ggcgggtgct ccaggtcggt catatcgagc ggttttccag
      601 cacataccgc gcactcgcgc gcgaggtcac gcgcccgctt tacattgaaa gctaccgcgt
      661 ctcaccgtgg aagcagagga gtggtgaggt cgacgtcgtt ctcgacctca tgattcatga
      721 catcgatctc attcaggggc tggtggggct gccggcaaca atggtgcacg cggtaggcat
      781 gccggtgatc aatgccacca cagatttggc gaatgccaga atcgtatttg gctccgcttg
      841 cgtcgccacc attacggcaa gccgaattag tcataagacc gagcggcgaa ttcgcgtttt
      901 tcagcccaat cggtacatca ttggcgattt cgcaaatcat cggatcgagc ggtattcggt
      961 ttgcggaaat ccggcacgga tagggctcgc cgcaatcaaa ttcgattccg tcgaggttcc
     1021 gaaagaggac agtctcgcca acgagatcgc cgagttcctc gaatgcatca gcactgggcg
     1081 cagaccgacg gtcacgggcc gcgaggcttg tgaggcgatg cgcctcgcgg agttgatcag
     1141 cgacagcatc gatgggcacc ggttgatggc gaacggagct cttcgaaacg acgctcaatg
     1201 ggacgagggc tcggcgccct acggtgcatc cgcatgaagc acgcctttgc actgcgcgat
     1261 acgaatcccg ctccggcccg ggtcaaggtg ccattgttcg atttgtcgcg tcaggctgag
     1321 cgtttcggtc ccaagctcaa ccaacgcatt gctgaagtgc ttcagcatgg ccggttcatt
     1381 ctcggcccgg aagtggagga gctcgaagct gcactcgcca ggtttacggg tgcgcgccac
     1441 gtcatcggtg tttcgagtgg tcgcgacgcc ttaatcattt cactgatggc gttgggcgtt
     1501 ggcgccggtg atgctgtttt cgttccggcg ttcacgttct ctgccacagc cggcgcggtc
     1561 gttgcggcgg gcgccgtgcc ggtcttcaca gatgtcgatc cggcaacctt gacgatggac
     1621 gccgaacagc tggagcgcgc aatcaagcgg acgctccgca atacggcgct gcgaccaagg
     1681 gcagttatcc cggtcgatct ctacggactt ccagctgact acgcggccat cgaagctgtt
     1741 gcgacgaggt atggcttatc gattatcgcg gacgcggcac agagcttcgg cgcccgggta
     1801 ggctcacgcc gggtcggcac tctggccgag attaccgcga ccagcttcta cccgacgaag
     1861 ccgctcggag ccttcggcga cggcggtgcc atcttcaccg atgacgacca actcgcccag
     1921 gcggtgcgcg agatcagaag ccacggcacc cgcggctcag gcgacgtcgc aatgcggctc
     1981 ggccttaccg gccgcctcga tacccttcaa gccgcggtgc tgttggtggg actagaggtc
     2041 ttcgaggaac ggctcgcctc gcgccgggag atcgcagccc gataccaacg ggccttcagc
     2101 ggtgtggtcg gaacccctcc gcagtcggcg cttctttgct ctgcctgggc gctctacacg
     2161 atccgaacac gggatcgaga taacgtgcgg gggcggcttg ctgcggcagg tgttgaaaca
     2221 ggcctttatt atcgcgtgcc tctccatcgc catcctgcct tcgcgggctg gctgaccggc
     2281 gaggaagagt tgccggtttc agagcaggca gcggatgagg tcttaagcct gccgcttcac
     2341 gcgggactga gcgaagagca
//
LOCUS       DASUMG010000294         2024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_680692, whole genome shotgun sequence.
ACCESSION   DASUMG010000294 DASUMG010000000
VERSION     DASUMG010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680692"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..685)
                     /locus_tag="VFX46_04850"
     CDS             complement(149..685)
                     /locus_tag="VFX46_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599939.1"
                     /translation="MARKQTRTPLLENEAEELENEAEERGVLAEHPARAAEPWEVVAE
                     VASRQGRAELAANGTRPLKHDGASRALAQETARADRNLGEPYMSLRGDPSHARGKVAP
                     HVERRGPSAGHHDHARRSQTVACCEASSPALRWAADQAQHRHPDEQRDAADNLDSGCP
                     KNLEFQRRGRNPAQPDSH"
     gene            730..1278
                     /locus_tag="VFX46_04855"
     CDS             730..1278
                     /locus_tag="VFX46_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599940.1"
                     /translation="MEVFVGQLAENIPRRMTALVPELVEVRVPKAQVRALAERLQGSG
                     TAYRHDVVVTKAMSVRLRAPEGGFWIETASPETQWIENTLGLLSDDYASWRWIVTPQR
                     PGKARLQLIVSARTVGADGLAAETALPDQVIEVKVRTNYQKVAIQWGGWIAAAGLGAI
                     LARFGEGIWDFGSALLTQIGLI"
     gene            complement(1288..1980)
                     /gene="yihA"
                     /locus_tag="VFX46_04860"
     CDS             complement(1288..1980)
                     /gene="yihA"
                     /locus_tag="VFX46_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX5599941.1"
                     /translation="MGPPRHKTPVVTSETEAASPDWIAAGAALFGRPWVFVRGVPALE
                     FLPEADRPEIAFAGRSNVGKSSLINAVVGQHGLARTSSTPGRTQELNFFATADVPLYI
                     VDLPGYGFAQAPKQKVKAWIELVRAYLSGRPTLMRVFLLVDSRHGIKPPDREIMQLLD
                     GAAVSYRIVLTKADKPNRSQLEAVVSATRAAIASHTAAHPDIIVTSATKGMGIDLLRA
                     TIAELYQTTRAA"
BASE COUNT          353 a          650 c          634 g          387 t
ORIGIN      
        1 ccgccgcgct ctgggaccga ggggcaaatt tcgcaggggt catcttccgc ccacgatcag
       61 cggaatgccc aaacgggctc aacaattgag aatattctcg ctgcggcgcg gcggcgcgtt
      121 gcggccggga tgaatggtgc acgtcagcct agtgagagtc cggctgcgcc ggattccgtc
      181 cgcgccgctg gaactcgaga tttttcggac agccactatc caaattatca gccgcatccc
      241 gctgctcgtc cgggtgccga tgttgagcct ggtccgcagc ccagcgcaga gctggagagc
      301 ttgcctcgca acatgccacg gtctgactcc ggcgggcatg gtcgtgatgc cccgccgatg
      361 ggcccctccg ttccacatgg ggtgcaacct tacccctcgc atggctcgga tcacctcgga
      421 ggctcatata cggttcccca aggttccgat ccgcgcgggc cgtctcctgc gccaaggctc
      481 gactggcccc gtcatgcttc agtggacggg taccattggc cgcaagctcc gcgcgaccct
      541 ggcgcgaggc gacctccgcc accacctccc atggctcagc agcccgcgca gggtgctccg
      601 cgagcacacc ccgctcctcc gcctcattct ccagctcctc cgcctcattc tccaagagag
      661 gcgtcctggt ctgcttccgc gccaccacct cctccgaagg cagccttcgg caatgagatg
      721 tctgccggcg tggaagtctt cgttggccag ctggccgaga acatcccgcg ccgaatgacc
      781 gctcttgtgc cggagcttgt cgaggtcagg gttcccaagg cgcaagtaag agcgctcgcc
      841 gaacgccttc aggggtcagg cactgcctat cggcacgatg tcgttgtgac gaaagcgatg
      901 tccgtgcggt tgcgcgcgcc ggagggcggc ttctggatcg aaacggcttc cccggagacc
      961 cagtggatcg agaacaccct cggtcttctg tcggatgact acgcaagctg gcgctggatc
     1021 gtaacgccgc agcgccctgg caaggcccgg ctacaactca tcgtttctgc ccgcacggtc
     1081 ggtgcggatg ggttggcagc tgagacggcg ctgcctgacc aggtcatcga agtgaaagtc
     1141 cggaccaact accagaaggt ggcgatccag tggggcggct ggattgcggc ggccgggctg
     1201 ggcgcaattc tggcccgatt cggcgaggga atttgggact tcggcagcgc gctgctgacg
     1261 cagattggtt tgatctgacg gccgatctca ggccgcgcgg gtcgtctgat acaattcggc
     1321 gatcgttgcc cgcaatagat cgatccccat acctttcgtc gccgaagtga cgataatgtc
     1381 cggatgggca gccgtatgag aagcgatagc ggcgcgagtg gcgctaacca cggcttccag
     1441 ttggctgcgg ttcggcttat ccgctttggt cagaacgatc cgataagaga cagcggctcc
     1501 atcgagaagc tgcatgattt cgcgatccgg cggcttgatc ccgtgccgtg aatcgaccag
     1561 caggaaaaca cgcatcaggg tggggcggcc actgagatac gcccgcacga gctctatcca
     1621 ggctttgacc ttttgcttgg gagcctgcgc gaagccgtag cccggcaaat cgacgatgta
     1681 gagcggcaca tctgctgtgg cgaagaaatt cagttcctgg gtgcgtccgg gtgtgctcga
     1741 cgtgcgagcc agaccgtgct ggccgacaac cgcgttgatg agactggact ttccgacgtt
     1801 ggagcggcct gcgaacgcta tttcgggccg gtctgcttca ggcagaaact ccaacgctgg
     1861 cacgccacgt acgaagaccc atggacggcc gaacagagca gccccggctg cgatccaatc
     1921 ggggctcgct gcctcagtct cgctcgtcac aactggtgtt ttgtgccgcg ggggccccat
     1981 cgttgcctcc tacggctaac cgctaccctt tagctttgcg cgcg
//
LOCUS       DASUMG010000295         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1082096, whole genome shotgun sequence.
ACCESSION   DASUMG010000295 DASUMG010000000
VERSION     DASUMG010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082096"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..624)
                     /locus_tag="VFX46_04865"
     CDS             complement(22..624)
                     /locus_tag="VFX46_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599942.1"
                     /translation="MCKIWTAGMRAVGFVLVSLMLNLAPASAQELSDRAVRAFMNYAW
                     SLTPAKFTKPDGTSVEIDKKDRSKVEVPMETAREVIKVGRLTAHAQICELPEDQIANY
                     RSLMRREEAKGKWTEQQIIYINQLHLTTVMLLTGKIKLVEKTEGGKEVVVKEPDEDEA
                     YTCTDEQRKKVKELIAAYVATGPDLNDNPKAAQAPAALKK"
     gene            complement(731..1987)
                     /locus_tag="VFX46_04870"
     CDS             complement(731..1987)
                     /locus_tag="VFX46_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5599943.1"
                     /translation="MRAVNPKHPRTQAPTVESTDVTVAEVVSAAYHETVEAASQLAEP
                     PLTRDEVRAAITFCADEACIAADSFCQGCRKWTEREGLTTFDQYCSRFSSIRFAESGL
                     RLEPGGPLPPVVFRSLDELAKSWAGEEFWFLARRVIRRLKKLEDPRPKRLTGIDEDAL
                     PSPTLILVRPQLADNIGMAARAMANFGLDDLRLVDPRDGWPNEKARAAASGANFIIDA
                     AKATGSLAEAVADLNFVCATTARQRDLAKPVLTPEQAVQEIHRRTELGQRCGIIFGPE
                     RAGLETDEVALADAIVMVPVNPRFASLNLAQAVLLVSYEWLKETRRGTLGRVTPYESA
                     LKPGLNTRSSRPATKEELYGFFDHLESELARLGFFNPPHRRQVVMSNVKSMFSRMNAT
                     EQEVRTLRGIVATLAHGKGAGRKTPS"
     gene            complement(2095..>2365)
                     /locus_tag="VFX46_04875"
     CDS             complement(2095..>2365)
                     /locus_tag="VFX46_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5599944.1"
                     /translation="EVALVKIDVEGHELSVLEGMRSLLQVRVPILVELTAERGNNERL
                     ERFKSLLCPFYEHVLDLSDESPVSRPSQSISAFRLRSSQSDLLIY"
BASE COUNT          437 a          739 c          701 g          488 t
ORIGIN      
        1 atggctgggt gaagagcgcc tttacttctt tagcgctgcc ggagcctgag cggccttcgg
       61 gttgtcgttg agatccggcc ccgttgccac gtatgcggca atcagctcct tcaccttctt
      121 acgctgctcg tcggtgcagg tataagcctc gtcctcatcc ggctctttga cgacgacttc
      181 cttcccaccc tcggtcttct cgacgagctt gatcttgcca gtcagcagca tcaccgtggt
      241 caggtgcaat tggttgatgt agatgatctg ctgctcggtc cacttgccct tggcttcctc
      301 gcgccgcatc agcgagcggt agttggctat ctggtcctcc ggaagctcgc agatctgcgc
      361 gtgggccgtg aggcgcccaa ccttgatgac ctcacgtgcc gtttccatcg gcacctcgac
      421 cttgctgcgg tccttcttgt cgatctcgac cgaggtgccg tccggcttcg tgaacttggc
      481 cggcgtgagg gaccaagcgt agttcatgaa cgccctgacg gcacgatcac tgagctcctg
      541 cgcgcttgca ggtgcgagat tgagcatcag cgagacgagc acgaacccta cagctcgcat
      601 cccggcggtc cagatcttgc acatttatcg atccttgaaa tctgctcacg tcgttgcggc
      661 ccgcaacaat tccgtcctcc cacagcattg tgcggtggtt cggctcgaaa gtaggcataa
      721 tcaaggcact ttaggatggt gtcttgcggc ccgcgccttt gccatgagca agcgtggcca
      781 ctatgccacg cagggtccgg acctcctgct cggtggcgtt catccgagag aacatggact
      841 tcacgttact catcacgacc tgccgccgat gcggcgggtt gaaaaatccg agccgggcga
      901 gctcggactc gagatggtcg aagaaaccgt agagctcctc cttggtcgcc ggacgggagc
      961 tgcgtgtgtt gagaccgggc tttaaggccg attcgtaagg ggttacgcgg ccaagggtac
     1021 cccgcctcgt ctctttcagc cattcgtagc tcaccagcag cacggcttgg gcaagattga
     1081 gcgaggcgaa acggggattg acgggtacca tgacgatagc gtccgcgagg gcaacctcgt
     1141 ccgtctcaag gcccgcccgt tccggaccga agatgatccc acagcgctga ccaagctccg
     1201 tgcgcctgtg aatttcttgg actgcctgct ccggcgtcaa cactggcttt gcgagatcgc
     1261 gttgccgcgc tgttgtggcg cagacgaaat tcaggtcggc gacagcctcg gccaagcttc
     1321 cagttgcctt tgccgcatcg atgatgaagt tcgcgcccga ggccgcggca cgggcttttt
     1381 cgtttggcca gccgtcgcgt ggatcgacca ggcgcaaatc atcgagcccg aagtttgcca
     1441 ttgcgcgagc ggccatgccg atgttgtccg caagctgcgg ccgcaccagg atgagtgtcg
     1501 ggctcggcag tgcatcctcg tcgataccgg tgagccgctt aggccgtggg tcctcgagtt
     1561 ttttcaagcg acgaatgacc cgacgcgcca gaaaccaaaa ctcctcgcca gcccaggact
     1621 tcgccagctc atcaagcgat cggaaaacga ctggcggcaa tggtccccca ggctccagcc
     1681 tcaggccgct ttctgcgaac ctgatggacg aaaaacgact gcaatactgg tcgaaagtcg
     1741 tcaggccctc acgttccgtc catttccggc agccctggca aaacgagtcc gcggctatgc
     1801 aggcttcgtc cgcacagaag gtgatggcgg cccgcacctc gtctcgcgtg agcggcggct
     1861 cggcaagctg gctcgcagct tccaccgtct cgtgataggc cgcgctcacg acctcggcga
     1921 cggtgacatc agtcgactct acggtaggag cctgcgtcct gggatgtttc gggttgacgg
     1981 cgcgcatcgt gcgggcctgc tgtcctcgtc atgaccgatc gtcggcagca ctcgcgtaac
     2041 gaatgccgct ggagagagca accatgcttg ccgccactcg gcgaagggca agcgtcagta
     2101 gatcagaaga tcggactggg aactacgtag ccgaaaagcc gaaatcgact gcgatggtcg
     2161 gcttacaggt gactcgtccg agagatccag cacgtgttca tagaaggggc acaacaagga
     2221 cttgaacctc tccagccgct cgttattgcc acgctcggcc gtgagctcga cgagaattgg
     2281 cactcggact tgcaggagcg accgcatgcc ctcaagaacg gacagctcgt ggccctcgac
     2341 gtcgattttc accagcgcca cctcc
//
LOCUS       DASUMG010000296         3870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_279108, whole genome shotgun sequence.
ACCESSION   DASUMG010000296 DASUMG010000000
VERSION     DASUMG010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279108"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VFX46_04880"
     CDS             complement(<1..756)
                     /locus_tag="VFX46_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5599945.1"
                     /translation="MNNRQERIAALHAQAAQRILIIDGAMGTMIQRHKLGEADYRGNR
                     FANHHKDVKGNNDLLVLTQPQIIADIHEQYLAAGADIIETNTFNAQRISQADYGMEEL
                     SYELNVAAARIAREAADKWTEKTPDRPRFVAGAVGPTNRTASISPDVNNPGYRNITFD
                     ELREAYAEQVRGLIEGGVDIILIETIFDTLNAKAAGFATLEVFDEAGFTLPIMISGTI
                     TDRSGRTLSGQTVEAFWYSLRHLKPFSVGLNCAL"
     gene            complement(753..1667)
                     /gene="metF"
                     /locus_tag="VFX46_04885"
     CDS             complement(753..1667)
                     /gene="metF"
                     /locus_tag="VFX46_04885"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEX5599946.1"
                     /translation="MNSERKSRLLGNGEITVSFEFFPPKNERMEEALWAAVRRLEPLN
                     PAFVSVTYGAGGSTRERTHATVSRLVRETTLSPAAHLTCVGATCEEIDSIVRSYWEAG
                     VRHIVALRGDPPGGVGTRYEPHPGGYANAAALVAGIAKIAPFEISVAGYPEKHPESPT
                     VEDDLDNLKAKVDAGASRVITQFFFDNERFLRFVEKARARGIWCPIVPGIVPIHNFKQ
                     VAGFAMKTGASIPAWLARRFEGLDDDPATTHLVAAVVAAEQVMDLVDEGIRQFHFYTL
                     NCADLVFAICHLLGLRPRPAVMQQAVTA"
     gene            complement(1664..2692)
                     /locus_tag="VFX46_04890"
     CDS             complement(1664..2692)
                     /locus_tag="VFX46_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5599947.1"
                     /translation="MALAGELEIERAVAGLKAAADSTRLRILLLLAEGELNVKDLTNI
                     LGQSQPRISRHVKLLGEAGLIERVREGSWVFCRLADGPGGALARALVQSVNANDPVYV
                     RDRQRAEEVKAEREMAAQAYFRAHAADWDRIRALHVSEAEVEAAMREALGTERVELLL
                     DLGTGTGRTLELFSDIYDRGIGIDLNQSMLAYARAKLRRTGLDHVQVRHGDIYNLSFP
                     DGVADAIVMHQVLHFLSDPSRAIKEAGRVLAPGGRLLIVDFAPHQLEFLRDEFAHERL
                     GFSSAQVSQWLADAGLSLVSSRDLPPPAGSESGKLTVSLWLATRPVVRQRSLARRSVA
                     RQSMESSL"
     gene            3562..>3870
                     /locus_tag="VFX46_04895"
     CDS             3562..>3870
                     /locus_tag="VFX46_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599948.1"
                     /translation="MAIQVAPRLAISRSRHSLIALLSALTLFLAAPSVRASETDPIEF
                     MKQTARELIAAARSGSQRDFMEVIQNRGHISAIGLYALGNYKSQLRPADRETYYGGIV
                     R"
BASE COUNT          760 a         1217 c         1137 g          756 t
ORIGIN      
        1 gagcgcgcaa ttgaggccga ccgaaaacgg cttcaaatga cgtaacgaat accaaaatgc
       61 ctcaaccgtc tggccagaca atgtgcggcc tgagcgatcg gtgatcgtgc ccgaaatcat
      121 gatgggcagc gtgaaaccgg cttcatcgaa gacttcgagt gtggcgaaac cggccgcctt
      181 ggcattcagc gtatcgaaaa tcgtctcgat caaaatgata tcgacgccgc cctcgatcag
      241 accacggacc tgctcggcat aagcctcccg caactcgtcg aaagtgatgt tgcgatatcc
      301 gggattattc acgtccggtg aaatggaggc ggtgcggttc gtcggcccaa cagctccggc
      361 gacaaagcgc ggacgatccg gcgttttctc ggtccatttg tctgctgctt cacgcgcaat
      421 ccgggctgcc gcgacgttaa gctcgtacga cagctcctcc atgccgtaat cggcctggga
      481 aatgcgctgc gcgttgaacg tgttcgtctc gatgatatcg gcgcccgcgg cgagatattg
      541 ctcgtgaata tcggcgataa tctgcggctg cgtgagcacc aacagatcgt tgttgccctt
      601 cacgtccttg tggtgattgg caaaccgatt gccgcgatag tccgcctcgc ccagcttgtg
      661 cctctggatc atggtgccca tcgcaccgtc gatgatgagg atgcgctgcg cggcctgcgc
      721 atgcagcgcc gcaattctct cttgccgatt gttcatgcgg tgacagcctg ctgcatgacc
      781 gccgggcgcg gccgcagtcc caagagatga cagatggcga acaccaggtc cgcgcagttc
      841 aacgtgtaga agtggaactg gcgaatgccc tcatcgacca gatccatgac ctgctcggcg
      901 gcgacaacgg ccgcgaccag atgcgtcgtc gccggatcat cgtccaaccc ctcgaaacgc
      961 cgtgcgagcc aggccggaat gctcgcgcct gttttcatgg cgaagcccgc gacctgcttg
     1021 aaattatgga tcggcacaat gcccggcacg atggggcacc agataccacg ggcgcgcgcc
     1081 ttctcaacga agcgcaggaa gcgctcgttg tcgaaaaaga attgcgtgat gacgcgcgag
     1141 gcgccagcat cgaccttagc tttcaagttg tcgagatcat cctcgaccgt tgggctctcg
     1201 ggatgcttct ccggatatcc cgcgaccgaa atctcgaatg gcgcgatctt ggcgatcccc
     1261 gccaccagag cagcggcatt tgcatagccg ccgggatgag gctcgtatcg tgtgccgacg
     1321 ccacccggcg gatcgcctct caaagccaca atgtggcgga cgccggcttc ccaatagctc
     1381 cgcacgatgg agtcaatttc ttcgcatgtg gcgcccacgc acgtcaggtg cgccgcgggc
     1441 gacaacgtcg tttcacggac gaggcgggaa accgtcgcgt gcgtgcgctc acgcgtggag
     1501 ccgccggccc cataggtcac agagacgaaa gcgggattga gcggctcgag gcgccgcaca
     1561 gcggcccaca acgcctcctc catgcgctcg ttcttcggcg gaaagaactc gaaggagacg
     1621 gtaatctccc cgttgccaag gagccggctt ttccgttcgg aattcataga gaggactcca
     1681 ttgactgacg ggccaccgac cttcttgcca aactccgttg ccggacgacc ggacgggtgg
     1741 caagccaaag tgacacagtg agtttgccgc tttccgagcc cgcgggcggc gggagatcac
     1801 ggctcgatac caacgacagc cccgcatccg ccagccactg gctgacttgg gccgatgaga
     1861 agcccagccg ttcatgggca aactcatcgc gaaggaattc gagctggtgc ggggcgaagt
     1921 ctacgatgag cagacgtccg ccgggcgcca gcacacgacc tgcttccttg atggcgcggc
     1981 taggatcgct caggaaatgc agcacctgat gcatcacgat ggcatcggcg acgccatcgg
     2041 ggaatgacaa gttgtagatg tcaccatgcc ggacctgcac atggtccagc cctgtgcgcc
     2101 gcagcttggc ccgcgcatag gcaagcatcg actggttgag gtcgatgccg atgccgcgat
     2161 catagatgtc cgagaacagt tcgagcgtcc tgcccgtacc cgtgccgaga tccagcagca
     2221 gctcgacccg ctccgtgccc agcgcttccc gcatggccgc ttcgacctcg gcctccgata
     2281 catgcaaggc acggattcga tcccagtccg ctgcgtgggc gcggaaatag gcctgcgccg
     2341 ccatttcgcg ctcggctttg acctcctcag cgcgctggcg gtcgcggaca taaacagggt
     2401 cgtttgcgtt caccgactgc accagggcgc gggcgagagc gcctcccggc ccatcagcaa
     2461 gccgacaaaa cacccagctc ccttcgcgca cccgttcgat gaggccagct tctcccagga
     2521 gctttacatg ccggctaata cgcggctggc tctgaccgag aatgttggtg aggtctttga
     2581 cgttgagctc gccttctgcg agcagcagga ggatgcgtaa gcgagtggag tcagcggccg
     2641 ccttcagacc cgccactgcc ctttcgatct cgagctcacc cgcaagcgcc atccgtgcct
     2701 ccttgtcgga ggagcatata aagatatctt tatgtccgtc aaggacatct taagtaatgc
     2761 ctgttccgga ggtgacagac gaaggaaccg ttggcctggt aaggagttgc cgtccccgcc
     2821 gttccggcag cctcccgacg aatagttgcg ttgatgagat tacaagaaat gccctccaac
     2881 gattttccgc gatcaacaaa tccgacaccg caagttgaaa gtccaacctc tgacagccgc
     2941 actggcagag gattgatcat tttcctcgtc ctcctgacag ccgctctcct gctgacgcca
     3001 tacgtgctcg tcgccgccgc ggcagcggcc cagccggaca tgcttcgcgt cctcgccgaa
     3061 aagcctttga ttgcggctca gctcgcaggc gaactcgtca tctctctgat gatctgcttg
     3121 ttgcccttca gccgaatggt ttcgcggcgt ccggcacgag ggtcaacgat cggcccagag
     3181 ccacactcgt ccgcaggcct cgcggatgct cagtcccctc gacaatcagc cgggcaagaa
     3241 cattgacgac ggcgcggtcc gtgctgagat catcttaggt ggccgcccct gggctctcgc
     3301 gcaacacctt ctcaaccttt tgagaagggt cggccgccgc gttcatccgg cgtcaacgta
     3361 agccatgtca ttcatccgat ctggtcaaat cgctaacggt ttgtacactg tgactgtgga
     3421 gacatggtag gtgcaaggct tacccggcgt cagccggctg cagttgacgg ggagagccac
     3481 ggctgtcaat ggatgccgtc ggcttggctg ctcgaggccc ggaacatgtt gtcggtccgt
     3541 ttcttgttcg ggagaattgg aatggccatt caagttgccc cgcgtttagc gatttctcgc
     3601 agccggcaca gcttgatagc gctgttatcc gcactcacgc tcttcctggc ggcaccttcg
     3661 gtgcgtgcct cggagaccga ccccatcgag ttcatgaagc agacggcgcg tgagctcatc
     3721 gccgctgcgc ggtcgggctc gcagcgcgat ttcatggaag tgatccaaaa ccgcggtcac
     3781 atttctgcga ttgggctcta tgcgcttggc aactacaagt cccagctgcg gcctgcggac
     3841 agggaaacct actacggcgg cattgtccgc
//
LOCUS       DASUMG010000297         2571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_680986, whole genome shotgun sequence.
ACCESSION   DASUMG010000297 DASUMG010000000
VERSION     DASUMG010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680986"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..563
                     /locus_tag="VFX46_04900"
     CDS             48..563
                     /locus_tag="VFX46_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5599949.1"
                     /translation="MTFRTETYLIKDDGTIPNNDRLPLVVYRSAISAEPGREAEERFK
                     TLFAQNGWGGTWVNGIYPFHHYHARSHEILGIAAGSVDVQFGGPGGPVVTLTAGDAVA
                     IPAGVSHCRRSESAGLSVVGAYPRGQEDWDLKRATEADRTQALEEIPKVALPDSDPIA
                     GRNGRLVELWR"
     gene            833..2392
                     /locus_tag="VFX46_04905"
     CDS             833..2392
                     /locus_tag="VFX46_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HEX5599950.1"
                     /translation="MDTIGVNRRTVLRHAGSWALASGVLAIATRTDWARAGEAENGED
                     DRAAFSGEVVKRLAMELAQRPFAKPEIELPEPFSKLSYDQYRDIRFRPEQAIWRGENL
                     DYELQLFALGYIYDTPVDIWLVEDGWMRKLKADGTLFSIGPLIPDAPQAAPFGFSGFR
                     IHGPINQSDVMDEYVVFQGATYFRAVGRSQRYGLSARGLAINTAKPPGEEFPIFRSFW
                     IEKPAPGAPEIIVHALLDSPSTTGAYRFAIRRGPFTVMDVEATLYPRTEIRHIGLAPL
                     TSMFLHGAAHRRINDDYRPAVHDSEGLAILNGRGERIWRPLTNPRLLQVSAFIDDDPK
                     GFGLVQRDRTYSSFEDLEAQYERRPTAWVEPKGNWGRGFVELIEIPTDEEIHDNIVAY
                     WKPANTVPAGEPLTFAYRLHWGEMIPVAWAGARVRKTRRGSNGRDGVLFVLDFDGPAL
                     ENASELPVADVSASAGTISNVVVHRHPAINGVRCSFELDPGSADLIELRLVLKANERV
                     ISESWLYRWTR"
BASE COUNT          497 a          786 c          825 g          463 t
ORIGIN      
        1 tgctcaagcc gcagctgcca tgtcgcaacg ttgaggcttg aactgccatg acgttccgga
       61 cagagacata cctgatcaag gacgacggca ctattcccaa caatgatcgt ttgccgctgg
      121 tcgtctatcg ctccgcaatc agcgcggagc cggggcggga ggccgaggag cggttcaaga
      181 cgctcttcgc gcagaacggg tggggcggaa cgtgggtcaa cggaatttat ccctttcacc
      241 attaccacgc gcgctcgcat gaaatcctcg gcatcgccgc ggggtcggtc gacgtgcagt
      301 tcggcgggcc tggcgggccg gtcgtgacgc ttacggccgg ggatgcggtc gccattcctg
      361 cgggcgtcag ccactgccgc cgaagcgaat cggcgggcct gagcgtcgtg ggagcctacc
      421 ctcgcggcca ggaggattgg gatctcaagc gcgccactga ggcagatagg acacaagcac
      481 tcgaagaaat tcccaaggtc gcacttcccg attcggaccc gatcgctggg agaaatggcc
      541 gtcttgtcga gctctggcgc tgaccggagc cgatgaatca gtcgcggaca tgacctggcg
      601 gcatgtctgc cccgatagcc gcggccggta cggcagcaaa accgccacat tgattgggca
      661 gcagagcacc catatggggc agcggaatcg gcgtgagttc aaggagttga acggacccgg
      721 cggcacaaca cgcagcgatt gcacgtgttg gatcggcctg tgagggccgg gggcgaggga
      781 ccgcgtgggc gcacgtctcg atgacgctga aggaactggg gccgaacgtc gtatggacac
      841 gattggtgtg aaccgtcgca ccgttctccg gcatgcggga tcctgggcgc tggcgagtgg
      901 agtcctcgct atcgcgacgc gtacagattg ggccagggcc ggggaggccg agaacggcga
      961 ggatgacagg gcggcgtttt ctggtgaggt cgtcaagcgc ctcgccatgg agctggccca
     1021 gcgtccgttc gccaagccgg agatcgagct gccggagccg ttctcgaagc tctcctacga
     1081 tcagtaccgc gacattcgct tccgtcccga gcaggcgatc tggcggggcg agaacctcga
     1141 ctacgagctg caactcttcg cgctcggata catctacgac acgcccgtag acatctggct
     1201 tgtcgaagat ggatggatgc ggaagctcaa ggccgacggc acgctgttct cgatcgggcc
     1261 actgatcccc gacgcgcctc aggccgcacc gttcggcttc tcaggctttc ggattcacgg
     1321 accgatcaat cagtcggacg tgatggacga gtacgtcgtc ttccaaggcg cgacgtattt
     1381 tcgagctgtc ggccgcagcc agcggtacgg tctttctgcg cgcggcctcg ccatcaatac
     1441 ggcgaagcca ccgggagagg agttcccgat cttccgcagc ttctggatcg agaagccggc
     1501 gcccggcgca ccggagatca tcgtccacgc gctgctcgac agcccctcga cgaccggcgc
     1561 ttatcgcttc gcaatccggc gcggcccgtt cacggtcatg gacgtggaag cgacgctcta
     1621 tccacggacc gaaatccgcc acatcgggct ggcacctttg acgagcatgt tcctgcacgg
     1681 tgcggctcat cgccgtatca acgacgatta ccgccctgct gtgcacgaca gtgaaggtct
     1741 cgcaattctc aacggccgcg gcgagcgtat ctggcgaccg ctgaccaatc cgcgactgct
     1801 gcaggtgagc gcctttattg acgatgatcc aaaggggttc ggcctcgtgc agcgcgaccg
     1861 cacatattcg agcttcgagg atcttgaagc ccaatacgag cgccgcccca ctgcatgggt
     1921 tgagccaaaa ggaaattggg gccgaggatt cgtggagttg atcgaaatcc caaccgacga
     1981 ggagatccac gacaacatcg ttgcctattg gaagcctgcc aataccgttc ccgcaggtga
     2041 gccattgacc ttcgcttatc gcctgcactg gggcgaaatg atcccggtgg catgggctgg
     2101 tgcccgcgtc cgtaagacgc ggcgtggcag caatgggcgc gacggtgtgt tgttcgttct
     2161 ggatttcgat ggcccagcac tcgaaaacgc gagcgagctt cccgtcgcag atgtgtcagc
     2221 gagtgctggc accatctcga acgtcgttgt ccaccgccat cctgcgatca atggagtgag
     2281 gtgcagcttc gagctggatc cgggatcggc ggatctcatc gaactcagac tcgttctcaa
     2341 ggccaatgag cgagtcatat ccgaaagctg gctttatcgc tggacgcgct gatcaatcat
     2401 gagcaacacg agcgccggcc gagcagccca agtggcggct caactcgaaa tcgaacgtcg
     2461 cgatgcgggc ctgagctggc ttccgcccga gcagccgctc gacatgccaa cgcaggactt
     2521 cgacgcgcca ccgtcctgcc ggctagcgcc aagagatgcc ggcatgcggt g
//
LOCUS       DASUMG010000298         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_200067, whole genome shotgun sequence.
ACCESSION   DASUMG010000298 DASUMG010000000
VERSION     DASUMG010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200067"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1137
                     /locus_tag="VFX46_04910"
     CDS             22..1137
                     /locus_tag="VFX46_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX5599951.1"
                     /translation="MSNSALFSPARLNGLDLPNRIVVSPMCQYSAEDGSASDWHMMHL
                     GMLSNSGAGLLIIEATGVEPAGRITPGCLGLYSDENEQALKRVVDACRKYGSAKIGIQ
                     LGHAGRKASTNPPWQGTPPGGPLKEGAWQTKAPSAIPFNEGWHVPKALTSQEIEELIE
                     AFAAAARRADRIGLDLIELHGAHGYLINEFLSPLTNKREDEWGGSLENRMRFPLEVFK
                     AVRAVWPQPKPLGIRISAVEWVDGGWTIEDSIELAKRLKDLGCDYIDVSSGGNNPNAR
                     IPIGPGYQVPFAEQIKKAVGIPVMTVGMITEAQQAESIIAEGKADFVAIARAFLDNPH
                     WAWHAAYKLGAEPMLPNQYLRVGPRVWPPAKQYAASA"
     gene            1250..>2482
                     /locus_tag="VFX46_04915"
     CDS             1250..>2482
                     /locus_tag="VFX46_04915"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5599952.1"
                     /translation="MYENLSLYIDGKFTAGDSGVTSDVINPANDSVLAKLPHAKKAEL
                     DAALAAADKGFKEWTRVSAYERAKILRKAADLVRERTDDIAKVLTQEQGKILIEARLE
                     VQGAADIIDWFAEEGRRAYGRIIPARADGVRNMVIMEPIGPVAGFAPWNFPVTQAVRK
                     IAAALAAGCSIIIKCPEETPGSPLGLVRCFHDAGVPAGVINLVYGTPAEISEYLIPSP
                     IIRKISFTGSVPVGKHLNALAASHMKRATMELGGHAPVLVYDDVDVEHVAKLMVALKY
                     RNAGQVCISPTRFFLQEKIYDKFVAEFTDIAKNLKVGDGMDPTTKMGPLANARRVNAI
                     EQFVQDAQEKGAKVATGGKRIGNQGNFFEPTVLTDVPENARIMNEEPFGPLAPITSFR
                     SYDEVVAEANRLPYGLAAY"
BASE COUNT          483 a          757 c          791 g          451 t
ORIGIN      
        1 ttcccagctt aggatcctca catgtcgaac agcgccttgt tctctcctgc gcgcctgaac
       61 ggcctcgatc tacctaatcg catcgtcgtc tcacccatgt gccagtattc tgcggaggac
      121 ggttccgcgt ccgactggca catgatgcat ctcgggatgc tgtcgaactc gggggccggt
      181 ctgctcatca tcgaggccac cggcgtcgag cctgcaggcc gaatcacgcc ggggtgcttg
      241 ggcctttact cagacgagaa cgagcaggcg ttgaagcggg tggtcgatgc ctgccgcaag
      301 tatggctcgg ccaagattgg catccagctt ggccacgccg gccgcaaggc atcaactaac
      361 ccgccgtggc aggggacgcc tccaggtggc ccgctgaagg agggcgcgtg gcagaccaag
      421 gccccctcgg cgatcccgtt caacgagggc tggcacgtgc cgaaggccct aacgtcgcag
      481 gagatcgagg agctgatcga agccttcgct gccgccgcac gccgagcaga ccggattggt
      541 ctcgatttga tcgagctgca cggtgcccat ggttacctca tcaatgagtt cctctcgccg
      601 cttacgaata agcgagagga tgaatggggt ggcagcctcg agaaccgtat gcggttcccg
      661 cttgaggtgt tcaaagcagt gcgtgcggta tggccacaac ctaagccgct tgggatccgt
      721 atttccgccg tggagtgggt cgatggcggc tggacaatcg aggactccat cgagctggca
      781 aagcggttga aggacctcgg ctgcgactac atagacgtct cgtcgggcgg taacaatccg
      841 aatgcgcgta ttccgattgg acctggctac caggtcccgt ttgctgagca gatcaagaag
      901 gcggtcggca tccccgtgat gacagtcggc atgattactg aggctcaaca agcggaaagc
      961 atcatcgccg agggtaaggc cgatttcgtc gccatagccc gcgctttcct agacaaccct
     1021 cattgggctt ggcatgcggc ctataagctg ggcgctgagc cgatgttgcc caatcagtac
     1081 ctccgggttg gacctcgggt gtggccgcct gctaaacagt acgcggcgtc tgcctgaagc
     1141 aacagagaca gtcacggaag ctcatgccta agcatgcggc ctgatctgca ggccgacaga
     1201 ggcgcgagct tcctgcacgg ggcgaaactg aacgttggaa agagcactca tgtacgagaa
     1261 tctcagcctc tacatcgacg gtaaattcac ggcgggtgac agcggcgtca catccgacgt
     1321 catcaatccg gcgaacgact ctgttcttgc caagctcccg catgcgaaga aggccgagct
     1381 cgatgcggcg ctcgcagcag ccgacaaggg cttcaaggag tggacgcgcg tgtcggctta
     1441 cgagcgggcg aaaatcctcc gcaaggctgc cgaccttgtc cgtgagcgga cggatgacat
     1501 cgccaaggtg ctcacgcagg agcagggcaa gatccttatc gaggcgcggc tcgaggtgca
     1561 gggcgcggcc gacattatcg actggttcgc cgaggaaggc cgccgtgcct acggccgcat
     1621 cattccggct cgcgctgatg gcgtgcgcaa catggtcatc atggagccga tcggtccggt
     1681 cgcaggcttt gcgccgtgga acttcccggt gacgcaggct gtgcgcaaga ttgcggcggc
     1741 tctggcggcg ggctgctcca tcatcatcaa gtgtccggag gagacgccag gctcacccct
     1801 tggcttggtg cgctgcttcc atgacgcagg cgttccggcg ggtgtcatca acctcgttta
     1861 cggcacgccg gcggagatct ccgagtacct gatcccgtcg ccgatcatcc gcaagatctc
     1921 gttcaccggc tcggtgcccg tcggcaagca cctcaacgcg cttgcggcca gccatatgaa
     1981 gcgggcgacc atggagctcg gcggccacgc gcctgtgctc gtctacgacg acgtcgatgt
     2041 ggagcacgtg gcgaagctca tggtggcgct gaagtaccgc aacgccggcc aagtctgcat
     2101 ctcgccgacg cgcttcttcc tgcaggagaa gatctacgac aagttcgtgg cggagttcac
     2161 cgacatcgcc aagaacctga aggtcggcga cggcatggat cccacgacca agatgggtcc
     2221 gctcgcaaac gcgcggcgcg tgaacgccat cgagcagttc gtgcaggatg cgcaggagaa
     2281 gggtgccaag gtcgccaccg gtggtaagcg cattggcaac cagggcaact tcttcgagcc
     2341 gactgtgctg accgacgtgc cggagaacgc ccgcatcatg aacgaggagc cgttcggccc
     2401 gctggcgccg atcacgtcgt tccgcagcta tgacgaagtg gtggccgaag ccaaccgtct
     2461 gccttacggc ctggcggcct at
//
LOCUS       DASUMG010000299         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1083095, whole genome shotgun sequence.
ACCESSION   DASUMG010000299 DASUMG010000000
VERSION     DASUMG010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083095"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..868
                     /locus_tag="VFX46_04920"
     CDS             86..868
                     /locus_tag="VFX46_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5599953.1"
                     /translation="MQIFKNPARERLQNGELALGIGLRQARTVDIAPAMKAAGFDWLF
                     IDLEHGSMGLDTAVQISVAALAAGISPIVRVPKGRYDLATRALDGGAFGIVMPHVDTA
                     DEAREIVSQLKYPPVGHRSIAGAMPQVGFQSVPLADAIAAVNSNMLIVAMLETPEAIA
                     NADAIAAVPGIDVLLIGTNDLTCEMGIAGALMHERVAEAYQTMVAACRKHGKWAGMGG
                     VYSEEGLRRYIEIGAQMILCGSDLSLLMSAGSVRTKFLRELG"
     gene            complement(886..1437)
                     /locus_tag="VFX46_04925"
     CDS             complement(886..1437)
                     /locus_tag="VFX46_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5599954.1"
                     /translation="MTRVQTQGVHHITLVGADRQTSIDFWEGVLGMPLVFEQPNLDAP
                     DQIHLYFDPGDGRLITVFTNESWKPDRTPNPTNVGNVHHIAFTVSRATYTQAAARLRE
                     RGFANSGEVDRGFMYSLYFRDPLGQLLELACYKFEPPTGYTHVDVLREAHKIRVARGA
                     YNITDEHLADAIEALSRRRSESS"
     gene            complement(1497..1712)
                     /locus_tag="VFX46_04930"
     CDS             complement(1497..1712)
                     /locus_tag="VFX46_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599955.1"
                     /translation="MAFEDIQAELGLLLTQLENERRPDDRHEIYLVIMRKLNELKAYG
                     MPLPQALVDLERNLEREFEAEQRGSAT"
     gene            complement(1799..2044)
                     /locus_tag="VFX46_04935"
                     /pseudo
     CDS             complement(1799..2044)
                     /locus_tag="VFX46_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088104.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA;
                     frameshifted; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
BASE COUNT          382 a          608 c          659 g          395 t
ORIGIN      
        1 tgatcaaggc ccattctgct aggcgctatt gcacaatcga tgtccttgct ttctcatgct
       61 cggggaggaa gagcgaggga aaggcatgca gatcttcaag aatccagcgc gtgaacgcct
      121 gcagaatggc gagcttgcac ttgggattgg gctgcgccag gcgcgaaccg tcgacattgc
      181 gccggcaatg aaagcggccg ggttcgactg gctgttcata gatctcgagc acggatcgat
      241 ggggctcgac acagctgtgc agatttctgt cgcggcgctg gcggccggga tttcacccat
      301 cgttcgcgtc ccaaagggcc gctatgatct tgcaacacgg gcgctcgatg gcggtgcatt
      361 tggcatcgtc atgccgcatg tcgacacggc cgatgaagca cgggagatcg tcagccaatt
      421 gaagtatccg ccggtcgggc atcgctcgat tgcgggggcc atgccgcaag tcggttttca
      481 gagcgttcca cttgccgacg cgatcgcggc ggtgaacagc aacatgctca tcgtggcgat
      541 gctcgaaacg cctgaggcga tcgccaacgc ggacgccatt gccgctgtgc cgggtatcga
      601 tgtcctcctg atcggcacca acgacctgac ctgtgaaatg ggcatcgccg gcgctctaat
      661 gcacgaacgc gtcgctgaag cctaccaaac aatggtggcg gcgtgccgga aacacggcaa
      721 atgggccggc atgggaggcg tctactcgga ggagggtctg cgtcgctaca ttgaaattgg
      781 cgctcagatg atcctctgcg gcagcgactt gagtttgctc atgagcgctg gatccgttcg
      841 cacgaagttc ttgcgagaac tcgggtagct gagcacgttg aaggctcaac tactctcact
      901 gcgccgccgc gagagcgcct cgatcgcgtc cgcgaggtgc tcatcggtga tgttgtaggc
      961 cccgcgggca acgcggattt tgtgggcctc gcgcagcacg tccacatgcg tgtagcccgt
     1021 cggcggctcg aacttgtagc aagcgagttc gagcaactgg cccaggggat cgcggaaata
     1081 gagcgaatac atgaacccgc gatccacctc gccggagttg gcgaacccgc gctcgcgcag
     1141 ccgtgccgct gcctgcgtat aggtcgcgcg ggagaccgtg aatgcaatgt ggtggacgtt
     1201 gccgacgttg gttgggttcg gcgtgcgatc cggcttccag gattcgttgg tgaaaaccgt
     1261 tatcagccgc ccgtcgccgg ggtcgaagta gaggtggatc tggtccggcg cgtcgaggtt
     1321 cggctgctcg aaaacgagcg gcatgccgag cacgccctcc cagaagtcaa tggaggtctg
     1381 gcgatccgct ccgacaagcg tgatgtgatg cacgccctga gtttgcacgc gggtcatcct
     1441 tttgctccgc agctttgatc cgcagtttcc gaccgataac ggcaaactgc ttacccttag
     1501 gtggcgctgc cacgctgttc ggcctcgaat tcgcgctcga ggtttcgctc gaggtcaacg
     1561 agagcctgag gcagcggcat gccatacgcc ttgagctcgt tcaacttgcg catgatcacg
     1621 agatagattt cgtggcggtc atcgggacgc ctctcgtttt ccagctgggt gagcaggagg
     1681 ccgagctccg cctggatgtc ttcaaaggcc atggtcgcac catcatccga tctgcccccc
     1741 acttcagact aaaccacgaa ccgtcgcgaa tcgcaccaat aggggcgtgc cgagccactc
     1801 aatgggcagc ctccagcgcg aggctaattc cctggccaac gccgatgcac acagcgctcg
     1861 ccgtgcctta cgctccttga gctccagcgc cgccgtgagc accaggcgag ctcccgacat
     1921 gccaaacggg tggccgagcg cgatggcgcc gccgttcgga ttgacgtggg gggcgtcgtc
     1981 gggaaggccg agctcgcgaa ggacgggcag ggcctgggcg gcgaaggcct cgttgatctc
     2041 gatc
//
LOCUS       DASUMG010000300         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_761931, whole genome shotgun sequence.
ACCESSION   DASUMG010000300 DASUMG010000000
VERSION     DASUMG010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761931"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..410
                     /locus_tag="VFX46_04940"
     CDS             174..410
                     /locus_tag="VFX46_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599956.1"
                     /translation="MNRPLAGVKLWAALLACVMGLALASCSRANSTRLPELATLPEGL
                     LTKEQQQEAVEKLQERRKVHREQAIEEIKSARRN"
     gene            539..1765
                     /locus_tag="VFX46_04945"
     CDS             539..1765
                     /locus_tag="VFX46_04945"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5599957.1"
                     /translation="MHEEFHRIKRLPPYVFAEVNRLKAEARAAGADIIDLGMGNPDLP
                     TPKHIIDKLVETVNKPRTNRYSASKGILGLRRAQAAYYERRFGVKLDPETQVIATLGS
                     KEGFANMAQAITAPGDVILVPNPTYPIHEFGFIISGASIRHIPASNGPEFLRGVERAA
                     KYSIPKPLAMVLNYPSNPTAMTVDLDFYAEAVQLAKRLGLIILSDLAYAEIYFDNNPP
                     PSVLQVPGAIDVAVEFTSLSKTYSMPGWRVGFAVGNERLIAALARVKSYLDYGAFTPI
                     QVAATAALNGPQDCVEEIRQVYKSRRDTLIESFARAGWHIPTPPATMFAWAPLPEPYR
                     DIGSVEFAKLLIEKADVAVSPGLGFGEYGEGYVRIALVENEHRIRQAARNIKRFLSQP
                     PELMHNVIPLQKKASS"
     gene            1774..3099
                     /locus_tag="VFX46_04950"
     CDS             1774..3099
                     /locus_tag="VFX46_04950"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5599958.1"
                     /translation="MAPPLRLGIAGLGTVGTGLIRLLDAHAERFRATLGRDITVTAVS
                     ARNRHKDRGISIDRFRWVDNPVELAADPGIDVFVELIGGENGIAAEAVEAALRAGKHV
                     VTANKALLARHGVRLARLAEERGIALNFEAAVAGGIPVIKTIREALLGNEISRVYGIL
                     NGTCNYILTTMQEEGRPFAEVLKEAQERGYAEADPTFDIGGFDTAHKLSLLTSLAFGT
                     ELAFEDIHIEGIQHITPADIEAADDLGYRIKLLGVAVRTPSGIEQRVHPAMVPKHSAI
                     AEVSGVTNCVAIDGDFVGDLLLVGSGAGAKPTASAVASDIGDIARGLVFPPFLTPASK
                     LKPYTRAILDRHQGAYYVRLTVYDRPGTMAAIASRMAEQDVSIESIVQRRPRSALPGS
                     GGRPAPGTLTPIVMITHETTEEAMRKALAAIERDGKVSEKPQMIRIEKL"
     gene            3139..4095
                     /gene="glpX"
                     /locus_tag="VFX46_04955"
     CDS             3139..4095
                     /gene="glpX"
                     /locus_tag="VFX46_04955"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX5599959.1"
                     /translation="MVNERSEGLDRVLVLEVGRVTEAAAIAAAKFRGRGNEKAADKAA
                     VDAMRRELNRLHIRGRVVIGEGEMDEAPMLYIGEEVGIGDGPEVDIAVDPLEGTTICA
                     KAMPNSLAVLAIAERGGLLHAPDMYMNKIAIGPGYPEGIIDLDASPADNLNALAKAKG
                     VPISEITACILDRPRHADLIKAVREAGAAVQLIPDGDIAGVIWTTDPEETGIDIYLGS
                     GGAPEGVLAAAALRCIGGQMQGRLMPLKDEERERAAKMGADIDRKYTMEDMASGDVIF
                     SATGVTDGSLLDGVHFRGDFAETETVIMRAKTGTVRRNVGVK"
BASE COUNT          839 a         1307 c         1339 g          728 t
ORIGIN      
        1 gggtgacatt cggccccgat ccggtggaag cacagcgtga ctgaatttat cgataagact
       61 tcgagacatt tagatagtgt tgcaagagta ccggcgccag cgtacaggcg aaccgtttca
      121 gaatcgatta aatttgacgc atgatttatt gcagcgccag caacgaagct cgagtgaacc
      181 ggcccttggc cggcgttaag ctctgggcgg cactcctcgc gtgtgtgatg ggattggcgc
      241 tcgcaagctg ctcgcgagcc aactccacgc gcttaccaga gctcgcaacg ctgccggaag
      301 gcctgctcac caaggagcag cagcaggagg ccgtcgagaa gctgcaggag cgccggaagg
      361 tccaccgcga gcaagccatc gaggagatca agagcgcccg gcgcaactga ccggaagctt
      421 tcttaaccga gggtttcacc acccggcccg ctccccggct ctggacgaag agaagcaaac
      481 gatcgtaaag acggacggcg gattttgtct gtgctgacac taaccgaggg ttgatcccgt
      541 gcacgaggag ttccaccgca tcaaacgcct gccaccttac gtttttgctg aggtcaatcg
      601 cctcaaggca gaagcacgag ccgccggtgc cgacatcatc gacctcggca tgggcaaccc
      661 ggacctgccg accccgaagc acatcatcga caagctggtc gagacggtga acaagccgcg
      721 caccaaccgt tactcggcct cgaaaggcat cctgggactc aggcgcgcac aagccgccta
      781 ttacgagcgc cgcttcggcg tgaagctcga tcccgagacg caggtgatcg ccaccctcgg
      841 ctccaaggaa ggcttcgcca acatggcgca ggccatcacg gcaccgggcg acgtcattct
      901 ggtgccgaac ccgacttatc ccatccacga gttcggcttc atcatctccg gagcatctat
      961 ccgccacatc ccggcgagca acggcccgga gtttcttcgc ggcgtcgagc gcgcggccaa
     1021 gtactcgatc cccaagccgt tggcgatggt gctgaactac ccgtcgaatc cgacggccat
     1081 gacggtggat ctggacttct atgcggaggc ggtacaactc gccaagcggc tgggcctcat
     1141 cattctgtcg gacctcgcct acgccgagat ctacttcgac aacaatccgc caccctcggt
     1201 gctgcaggtg ccgggtgcaa ttgatgtcgc agtcgagttc acctcgctct cgaagaccta
     1261 cagcatgcct ggctggcgtg tgggcttcgc ggtggggaac gaacggctca tcgcggcgct
     1321 ggcacgagtc aaatcttatc tcgactacgg tgccttcacg ccgatccagg ttgcggccac
     1381 ggcggcgctc aacgggccac aggattgcgt cgaggagatt cggcaggtct acaagagccg
     1441 ccgcgatacc ttgatcgaga gcttcgcccg agcgggctgg cacattccca ctccgccggc
     1501 aaccatgttc gcgtgggcgc cgcttcccga gccctaccgc gacatcggct cggttgagtt
     1561 tgcgaagctg ttgatagaaa aggcggatgt ggctgtctca cccgggcttg ggttcggtga
     1621 gtatggcgag ggctacgtgc gcatcgcgct ggtggagaac gagcaccgga tccgccaggc
     1681 ggcgcgcaac atcaagaggt tcctctcgca gccacctgag ttgatgcaca acgtcattcc
     1741 actgcagaag aaggcctcca gctagtctcg cacatggcac cacctttaag actcgggatt
     1801 gccggcctgg gtaccgtcgg cactggcctc atccgactgc tcgatgctca cgcagagcgg
     1861 ttcagggcga ccctcgggcg tgacatcacg gttacggccg tgtcggcgcg caaccgtcac
     1921 aaagaccggg gcatctcaat cgatcgtttc cggtgggtgg acaatcccgt cgagctggcg
     1981 gccgatccag gcatcgatgt gttcgtcgag ttgatcggcg gggaaaatgg catagccgcc
     2041 gaggcggtcg aggccgccct ccgggccggg aagcacgtgg tgaccgcgaa caaggcgctg
     2101 ctcgcccgcc acggcgtccg gctggctcga ctggccgagg aacgcggcat cgccctcaat
     2161 ttcgaggcgg ccgtcgctgg cggcataccc gtcatcaaga cgatccggga ggcgctgctc
     2221 ggcaacgaga tcagccgcgt gtacggcatc ctcaacggca cgtgcaacta catcctcacc
     2281 acaatgcagg aggaggggcg gcccttcgct gaggtgctga aggaggccca ggagcgcggc
     2341 tacgcggagg cggatccgac cttcgacatc ggcggcttcg acacggccca caagctgtcg
     2401 ctgctgacga gcctggcgtt cggcacggag ctggcgttcg aggacatcca catcgagggc
     2461 attcagcaca tcacaccggc tgacatcgaa gcagcagatg atcttggcta ccgcatcaag
     2521 ctgctgggtg tcgccgtgcg cacgccgagt ggcatcgagc agcgggtcca tccggcgatg
     2581 gtgccgaagc actccgctat cgctgaggta tccggcgtta cgaactgcgt cgcgatcgac
     2641 ggcgatttcg taggagacct tcttttggtg ggctcgggcg ccggcgccaa gccgaccgcg
     2701 tccgctgtgg cgagcgacat cggcgacatc gcccgcgggc tggtcttccc gccgtttctc
     2761 acacccgcct cgaagctcaa gccctacacc cgcgccattc tcgaccgaca ccagggcgcc
     2821 tattacgtgc ggctcaccgt ctacgatcgc ccgggaacca tggcggcgat cgccagccgg
     2881 atggcggagc aggacgtttc catcgaaagc atcgtgcagc gccggccccg ctcggccctg
     2941 ccggggtcag gaggtcggcc ggcgccaggc actctgaccc cgatcgtgat gatcactcac
     3001 gagacgaccg aagaagccat gcgcaaggcg ctggccgcga ttgagcgcga cgggaaggtt
     3061 tcagagaagc cgcaaatgat tcgcatcgaa aaactctgat acatcggact ggcgagatcg
     3121 gagatggagg aaggccccat ggtcaacgag cgtagcgagg gtttggatcg tgtgctcgtc
     3181 ctcgaggttg ggcgggtaac ggaggccgcc gccatcgccg cagccaagtt cagggggcgt
     3241 ggcaacgaga aggcagccga caaagctgcc gtcgatgcaa tgcgccgcga gctcaatcgg
     3301 ctccacatcc gggggcgggt cgtcatcggc gaaggcgaga tggacgaggc cccgatgctc
     3361 tatatcggcg aggaggtcgg catcggggat ggtccagagg tcgatattgc ggtcgatccc
     3421 ctcgagggca ccacgatttg tgccaaggcg atgccgaatt cgctggccgt cctggcgatt
     3481 gccgagcgcg gtggtcttct ccatgcgccg gacatgtaca tgaacaagat cgccatcggc
     3541 cccggttatc ccgagggcat catcgatctc gatgcctcgc ctgcagacaa tctcaacgcg
     3601 ttggccaagg cgaagggcgt tccgatcagt gagatcacgg catgtatcct cgaccggccg
     3661 cggcatgccg acttgatcaa ggctgtgcgg gaggctggcg ctgccgtgca gctcattccc
     3721 gacggtgata tcgccggcgt catttggacg accgaccctg aggaaaccgg catcgacatc
     3781 tatctcggct ccggcggcgc gccggaaggc gttctggcag ccgctgcgct gcgctgcatc
     3841 ggcggtcaga tgcaagggcg gctcatgccg ctcaaggatg aggagcgcga gcgcgccgcc
     3901 aagatgggtg ccgacataga ccgcaagtac acgatggagg acatggcctc cggcgacgtg
     3961 atcttctccg cgacaggtgt tacggatgga tcgctgcttg atggcgtcca cttccggggt
     4021 gacttcgcgg agacggagac cgtcattatg cgcgccaaga ccggcacggt gcgccgaaat
     4081 gtcggagtca aatgaggaaa acgcaaagga tcactatgga gaaatcgata gagctctctg
     4141 tcgcaaagtt gctcaactcc tttccgagtt agaccaagag ctatatcgga gactggagaa
     4201 gtccggaagc tat
//
LOCUS       DASUMG010000301         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1163944, whole genome shotgun sequence.
ACCESSION   DASUMG010000301 DASUMG010000000
VERSION     DASUMG010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163944"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /gene="pgk"
                     /locus_tag="VFX46_04960"
     CDS             complement(<1..452)
                     /gene="pgk"
                     /locus_tag="VFX46_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX5599960.1"
                     /translation="MDLDKLKTTAGLDVHGKRVLVRADLNVPVQDGQVTDATRLERLI
                     PGIRDLSSRGARVVIISHFGRPKGNWAPEFSLKPVADKLSDLMGTPISFVEDCVGPTA
                     QQAVNSLSPGQIVVLENLRFHKGEEKNDPEFAKELAALGDLYVNDAFS"
     gene            complement(477..>2273)
                     /gene="tkt"
                     /locus_tag="VFX46_04965"
     CDS             complement(477..>2273)
                     /gene="tkt"
                     /locus_tag="VFX46_04965"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5599961.1"
                     /translation="SAGHGSMLLYSLLYLTGYPGMDIEQIKQFRQLGSRTAGHPEYGH
                     APGIETTTGPLGQGIATAVGMAIAERIWNARLGPDVIDHHTYVVAGDGCLMEGISQEA
                     ITLAGHLGLGKLIVLFDDNGISIDGPTSLSTSEDQIRRFEAAGWNTAAVDGHDPDAVA
                     AAISRARSDDSKPWLIACRTTIGFGAPKKQGTAGVHGSPLGSDEIAAARQTLGWPHPP
                     FEVPEPILKAWRSAGRRGAEERARWQERWAKIDAKLRESFEHPVAAHGEALRQAILEA
                     KRKAAAETAKKATRQWSEFTLDYLVPVLPGLIGGSADLTGSNNTKTKSQTVIRKGDFS
                     GTYIHYGIREHAMAAAMNGLALYGGLIPYGGTFLVFSDYCRPAIRLSALMRQRVIYVM
                     THDSIGLGEDGPTHQPIEHLASLRAMPNLNVLRPADGVETVECWEIAVRPSDTPSILA
                     LTRQPVPNLRTTHTDENLCAKGAYVLRETSGKRDVTLLATGSEVGLAVEAAEALAGQG
                     IAAAVVSMPCFELFRQQPEGYRRAVLGDAPRIGIEAAVVQGWHEWLDKDDVFIGLSDF
                     GASAPGAALFQHFGLTPARVVETALALIAKRR"
BASE COUNT          424 a          746 c          682 g          421 t
ORIGIN      
        1 ctggaaaacg cgtcgttgac gtagaggtcc ccgagtgccg ccagctcttt cgcgaactcg
       61 ggatcgttct tttcctcgcc cttgtggaac cgaaggtttt ccaacaccac gatctgacca
      121 ggcgataagg agttcaccgc ctgctgagcc gtcggtccaa cgcaatcttc cacgaaagaa
      181 atcggtgtgc ccatcagatc ggacagcttg tcggcgacag gtttcagcga gaactccggt
      241 gcccaatttc ctttcggccg tccaaagtgc gagatgatga cgacgcgagc gccgcggctc
      301 gacaagtctc gaatgccagg aatcagccgc tcgagccggg tcgcatccgt cacctggccg
      361 tcctgcacag gaacgttgag atcggcgcgc accagaacgc gcttcccatg aacgtcgaga
      421 cctgccgtcg tcttgagctt atcgaggtcc atggacgatc tcccattcct gtgatgtcac
      481 ctgcgtttcg cgataagggc cagcgccgtt tcgacaaccc gcgcaggcgt caggccgaag
      541 tgttggaaca aggccgcgcc cggcgctgaa gcaccgaaat cgctgagccc gatgaacacg
      601 tcatccttgt cgagccactc atgccagccc tggacgacgg ccgcttcgat gccgatccga
      661 ggagcatctc ccagcacagc gcggcgatag ccttccggct gctggcggaa aagttcgaag
      721 cacggcattg aaaccacggc cgccgcgatc ccttgcccag ccagcgcctc tgccgcctcg
      781 accgcgagtc cgacctccga ccccgtggcc agcaaggtga cgtcgcgctt gcccgaagtt
      841 tcacgcaaga catacgcgcc tttggcgcaa aggttctcgt cggtgtgcgt cgtgcgcagg
      901 ttcggcacgg gctgccgagt cagcgcgagg atgctgggtg tatcgctcgg gcgcacagcg
      961 atctcccagc actcgaccgt ctcgacccca tccgccgggc gcaggacgtt gaggttcggc
     1021 atcgctctga ggctcgcgag atgctcgatc ggctggtgcg ttggcccatc ctcgccgaga
     1081 ccgattgagt cgtgggtcat tacgtaaatg accctctggc gcatcaaagc tgaaaggcga
     1141 atggccgggc ggcaatagtc agagaacacg aggaaggtgc cgccatacgg gatcaggccg
     1201 ccgtaaagcg ccagcccgtt catggccgct gccatggcgt gctcgcgaat gccgtaatgg
     1261 atgtaggtgc cgctgaaatc gcctttcctg atgacggtct gcgatttggt cttggtgttg
     1321 ttcgagcccg tcagatcggc tgagcctccg atcaaacccg gcaagaccgg caccaggtaa
     1381 tccagcgtga attcggacca ctgacgtgtc gccttcttgg cggtttcagc cgcggccttg
     1441 cgtttggcct caaggattgc ctgccgaaga gcctcaccat gcgcagccac tgggtgctcg
     1501 aagctctcgc gcagcttggc atcgatcttg gcccaacgct cctgccatct cgcccgctcc
     1561 tcagccccgc ggcgcccagc ggagcgccaa gctttcagaa ttggctccgg cacctcgaaa
     1621 ggcggatgag gccacccaag cgtctgtctc gccgctgcga tttcatcact accgagcgga
     1681 gagccgtgca cgccggccgt gccttgcttc ttcggggcgc caaacccgat cgtggtcctg
     1741 caggcgatga gccagggttt gctgtcatca gaacgggcgc gcgagatagc ggcggccacg
     1801 gcgtccggat catgcccatc gaccgcagcc gtgttccaac cagcggcctc gaagcgccga
     1861 atctgatcct cggaggtgga aagcgacgtc ggcccgtcga tggagatccc gttatcgtcg
     1921 aacagcacaa tcagcttgcc cagccccagg tgacccgcaa gcgtgatggc ttcctggctg
     1981 atgccttcca tcaggcagcc atcgcccgca acaacatacg tgtggtgatc gataacgtcg
     2041 ggtccgaggc gcgcgttcca gatccgctct gcaatcgcca tgccgaccgc ggtggcaatg
     2101 ccctggccta gcggtccggt agtcgtctca atacccggcg catgcccgta ctcaggatgc
     2161 cccgccgtcc tcgagccgag ctgccgaaac tgcttgatct gctcgatgtc catgcccgga
     2221 tagccggtca ggtagagcag cgaataaagc agcatggagc catggccggc cga
//
LOCUS       DASUMG010000302         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_440558, whole genome shotgun sequence.
ACCESSION   DASUMG010000302 DASUMG010000000
VERSION     DASUMG010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440558"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VFX46_04970"
     CDS             complement(<1..375)
                     /locus_tag="VFX46_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX5599962.1"
                     /translation="MPKRTDLRSILIIGAGPIVIGQACEFDYSGAQACKALRQEGYRV
                     ILVNSNPATIMTDPDLADATYIEPITPDVVARIIAKEKPDAVLPTMGGQTALNTAMAL
                     HRSGKLKEFGVELIGAQVESIKV"
     gene            complement(359..703)
                     /locus_tag="VFX46_04975"
     CDS             complement(359..703)
                     /locus_tag="VFX46_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEX5599963.1"
                     /translation="MTTTRARQLRARQTDVERKLWFLLRDRRLNGAKFRRQVPIGNYF
                     VDFVCQEAKLIVEVDGSQHSDQVAYDCARTEWLRSVGYRVLRIWNNDLTENEEGVLTA
                     ILNELDPNAQAH"
     gene            complement(855..2021)
                     /gene="carA"
                     /locus_tag="VFX46_04980"
     CDS             complement(855..2021)
                     /gene="carA"
                     /locus_tag="VFX46_04980"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX5599964.1"
                     /translation="MSAPHATDPTHAPTALLVLGDGTVLKGRGLGAEAKAVGEVCFNT
                     SMTGYQEIMTDPSYAGQIINFTFPHIGNVGANAEDIETVTPAARGCVLAIDITEPSSW
                     RALQSFDEWLRRYNLPAIAGIDTRRLTRRIRDGGAPNGVIVHRRDGAFDVPALVKEAK
                     AWPGLEGMDLAKDVSCRQTYHWDESLWALGRGYGKQSHPKFKVVAIDYGAKRNILRCL
                     TAAGCDVTVVPATATTGDVLRHKPDGVFLANGPGDPAATGEYAVPVIKDILKRDIPIF
                     GICLGHQMLALALGGKTEKMPRGHRGANHPVKDLTTGKVEITSQNHGFCVVRESLPKG
                     VTVTHESLFDRCVEGLELEGKPVFSVQYHPEASPGPMDSHYLFHRFVEKMEERK"
     gene            2328..>2442
                     /locus_tag="VFX46_04985"
     CDS             2328..>2442
                     /locus_tag="VFX46_04985"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX5599965.1"
                     /translation="MLRQRLNDDLKAAMKSRDQRATSALRLILAALKDRDIA"
BASE COUNT          386 a          766 c          805 g          485 t
ORIGIN      
        1 aaccttgatc gactcgacct gcgcgccgat cagctcgacg ccgaactcct tgagcttgcc
       61 ggagcggtgc agcgccatcg cggtgttgag ggccgtctgc ccgcccatgg tcggcagcac
      121 cgcgtccggc ttctccttcg cgatgatgcg cgccaccacg tccggcgtga tcggctcgat
      181 ataggtcgcg tccgcgaggt ccggatcggt catgatggta gcgggattcg agttcaccag
      241 gatgacgcga tacccttcct gccgcagcgc cttgcacgcc tgcgccccgg aataatcgaa
      301 ctcgcaggcc tggccgataa cgatggggcc ggcgccgatg atcaggatgg agcggaggtc
      361 agtgcgcttg ggcattcggg tccagctcgt tcaagattgc ggtcagcacg ccttcctcgt
      421 tctctgtgag atcgttgttc cagatgcgca gaacgcgata tccgactgaa cgcagccact
      481 ctgtacgcgc gcaatcgtaa gcgacttgat ctgaatgctg gctgccatcg acttcgacga
      541 tcaacttcgc ttcctggcag acgaagtcga cgaagtagtt gccgataggg acttggcgac
      601 gaaacttcgc gccattcaag cgcctatcgc gcagcaggaa ccaaagcttg cgctccacgt
      661 cggtctgtcg tgctctcaac tgacgtgcgc gcgtcgtcgt catgatggca gtcctcgcct
      721 gcgatacccc tcaccctgtc cctctccctc aaggggagag gggacgctgt tgctgggctc
      781 gtgcgaatag cccctctccc ctcgtgggag aggggttggg gtgagggggg aagcgacggc
      841 tgcttccggc ttcatcactt cctttcctcc atcttttcga cgaagcggtg gaagaggtag
      901 tgggagtcca tggggcccgg agaggcttcc gggtggtatt gcacggagaa gaccggcttg
      961 ccctcgagct cgaggccctc gacgcacctg tcgaacagcg actcgtgcgt gaccgtcacg
     1021 cccttgggca agctttcgcg cacgacgcag aagccgtggt tctggctggt gatctcgacc
     1081 ttgccggtgg tgaggtcctt caccggatgg ttggcgccgc ggtggccgcg gggcatcttc
     1141 tcggtcttgc cgcccaaggc gagcgccagc atctggtggc cgaggcagat gccgaagatc
     1201 gggatgtcgc gcttgaggat atccttgatc accggcaccg catattcgcc agtcgccgct
     1261 ggatcacccg ggccgttggc gaggaacacg ccgtcgggct tgtggcgcag cacgtcgccg
     1321 gtcgtcgcgg tcgcgggcac cacggtcacg tcgcagccgg cggcggtgag gcagcgcagg
     1381 atgttgcgct tggcgccgta gtcgatcgcc accactttga acttcggatg gctctgcttg
     1441 ccgtagccgc ggccgagtgc ccacaggctt tcgtcccagt gataggtctg gcggcaggac
     1501 acgtccttgg cgaggtccat gccttcgagg cccggccagg ctttcgcttc cttgaccagg
     1561 gccggcacgt cgaacgcgcc gtccctgcga tgcacgatga cgccgttggg cgcgccgccg
     1621 tcgcggatgc gccgggtcag gcgccgcgta tcgatgccgg cgatggctgg caggttgtag
     1681 cgccgcagcc attcgtcgaa ggattgcagg gcgcgccagc tcgacggctc ggtgatgtcg
     1741 atggcgagca cacaaccgcg cgccgccggc gtcacggtct cgatgtcctc ggcattggcg
     1801 ccgacgttgc cgatgtgcgg gaaggtgaag ttgatgatct gaccggcgta ggagggatcg
     1861 gtcatgatct cctggtagcc ggtcatcgag gtgttgaagc agacctcgcc caccgccttg
     1921 gcttcggcgc ccaggcctcg gcccttcagc accgttccgt cgcccaggac gagaagggcg
     1981 gttggcgcgt gcgtagggtc ggtcgcgtga ggagcgctca tgccgatggc cttcggagtg
     2041 gaaaatgccg ctggaccttg ccggtccggc gcgcttgtcc gcatcccagg ggttagccat
     2101 tgggcgaacc cgaggccggc ggtccattcg acgatcggcg aagatcgccg gtttctaagc
     2161 cgcgaatcct gctccgtcaa gcgaatgtcc tgcttttttc ggcgccaaat cagtagatga
     2221 ttcaaccgtt tagtcagttc tcgggatggg cttccggaaa ttgcctcggt tccgggaatc
     2281 ctgtagtctc gccgcctttc acgataccct ctcgatccgg agttcccatg ttgcggcagc
     2341 gcctcaacga tgacctgaag gcggcgatga aatcgcgcga ccagcgcgcg acctccgccc
     2401 tgcgcctgat cctggccgcg ttgaaggacc gcgatattgc cg
//
LOCUS       DASUMG010000303         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1004039, whole genome shotgun sequence.
ACCESSION   DASUMG010000303 DASUMG010000000
VERSION     DASUMG010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004039"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VFX46_04990"
     CDS             <1..385
                     /locus_tag="VFX46_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599966.1"
                     /translation="QDAHRAPRASEERGRIDMFAPLDAECKGDSTKGFARFSLNAGCC
                     SQKVGRLWLESWSWLPLGWRDFRGRCNERSDLDQCRQGNKAILGEARGAAILSASQKD
                     RPPHWLKKIRLDVRYLGRSISCKEL"
     gene            437..664
                     /locus_tag="VFX46_04995"
     CDS             437..664
                     /locus_tag="VFX46_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599967.1"
                     /translation="MDIDRLTKAANAAGFAMANSEELLVPAPQPKPSEEFGWRVADPL
                     QQNEQQKALVSVKPALSLRDWWLALSTKATA"
     gene            complement(666..1559)
                     /locus_tag="VFX46_05000"
     CDS             complement(666..1559)
                     /locus_tag="VFX46_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5599968.1"
                     /translation="MTPFLSSSGPDRVLRLEDGRRLAYREYGARDGWPVLALHGTPGS
                     KLMFSIAEAAALELGLRLIAPDRWGYGKTDPHPRPSLEAWARDAAELVDALELSQLSL
                     VGISGGSPYAVATAAHLGDRIARMALAVPVGPIAGNPVEHRLAPFHRFIFLGVGRRPG
                     VTRFFFRGYRRLVAWKPRQAVMLVAVTQCPSDRALLRTPAVQEFLSTMLRDGLEPGVQ
                     GPAVDLELFASPWNFPLDRISSATRIWIGNDDQLVPLSAARHLASAIPNAELTELEGC
                     GHFWIAQDYRQVLDWLAESRP"
     gene            1771..2202
                     /locus_tag="VFX46_05005"
     CDS             1771..2202
                     /locus_tag="VFX46_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5599969.1"
                     /translation="MIRILSVAAAIAVSATVVYAGADFIDQRQRAMKALANAAKAPGA
                     VLKGEAKFELDKVHSSLAVFKEQAAKLKDLWPDDSKDGETNALPAVWDKKSEFLALFD
                     KFEADVQTASEKIKDEDSFKAEWPKVMSNCGGCHKQYRKPQ"
     gene            2343..>2737
                     /locus_tag="VFX46_05010"
     CDS             2343..>2737
                     /locus_tag="VFX46_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5599970.1"
                     /translation="MKRRSFLTVLLRLLVTPIVIAGAALYLLTIPSVLSESELAPRTA
                     DLANGETMFHAGGCASCHATPGEEGKTRLGGGLALNTAFGTFRAPNISPDPQYGIGSW
                     SEIEFVNAMLRGVGRNGEHLYPSFPYTSY"
BASE COUNT          574 a          825 c          821 g          517 t
ORIGIN      
        1 tcaggacgcg caccgcgcgc cccgcgcctc cgaggagcgc ggcaggattg atatgttcgc
       61 gcccctggat gccgagtgca agggggattc gaccaaaggt tttgcaaggt tttctctaaa
      121 tgcgggctgc tgctcgcaaa aagtcggccg gctttggctg gagagctggt cttggcttcc
      181 gcttggctgg cgcgactttc gaggccgatg caatgaacga tctgacttag atcaatgtcg
      241 gcaaggcaac aaagcaatac tgggagaagc gcgcggtgca gcaatcttga gtgcatcgca
      301 aaaagatcga ccaccgcatt ggttaaagaa aattcgtctt gacgtgcgat atttgggccg
      361 cagtattagc tgtaaggaac tttgaaagcc ggacggtgtt ttcagcttgc gtgcggtatg
      421 taggggcgtg ggtccaatgg atatcgacag gctgacaaaa gctgctaatg ccgccggctt
      481 cgctatggcc aattcggagg aacttctggt tcccgcgccg cagccgaaac caagcgaaga
      541 attcggttgg cgagttgccg atccccttca acaaaacgag cagcaaaagg cgctcgtatc
      601 cgtaaagcca gctctcagcc ttcgcgattg gtggttggcg ttatcgacca aggctacggc
      661 atagcttacg gccggctctc agcgagccaa tccaacactt gtctgtaatc ctgcgcgatc
      721 cagaaatggc cgcagccttc gagctcggtc agctccgcgt ttgggattgc ggacgcgagg
      781 tggcgggccg ccgatagcgg caccagctgg tcatcattgc cgatccaaat tcgagttgcg
      841 gagctaattc ggtccagcgg aaagttccac gggctggcaa acagctcgag atcgaccgca
      901 gggccctgga cgcccggctc caaaccatcg cggagcatcg tgcttaagaa ctcttgaacg
      961 gctggcgtgc ggagcaatgc tcgatccgat gggcattggg ttaccgccac cagcatcaca
     1021 gcctgtcgcg gcttccacgc caccagccgc cggtaccccc gaaagaagaa gcgcgtgaca
     1081 ccgggccgcc gtccgacgcc taaaaagatg aagcgatgaa acggcgcgag tcgatgctcc
     1141 acagggtttc ccgcaatggg gccaacggga acggcaagcg ccattctcgc gattcggtcg
     1201 cccaggtgcg cagccgtcgc cactgcgtaa ggtgatccgc ccgaaattcc caccagcgag
     1261 agctgtgaca actcgagcgc gtcgacgagc tcggccgcat cgcgagccca tgcttccagc
     1321 gacggacgcg ggtgaggatc agtcttccca tatccccaac gatccggcgc gatgaggcgc
     1381 aggccaagtt caagagcggc cgcttccgca atgctaaaca tgagctttga gcctggcgtc
     1441 ccgtgcagcg ccaacaccgg ccatccatcg cgcgcgccat actcccggta cgccagtcgc
     1501 cggccatcct ccaggcgcag cacgcgatcc ggaccagagc tgctaaggaa cggggtcatt
     1561 ctgggctccg atgtttagtc agactcttct gtagagctct cgcgttcgta gattgacaag
     1621 ccacgtcagg atccgcgccc aaggccctgg atcggcagcc atttcacgtg gctgtgatcg
     1681 agaatgggcc gcctcgcaat tgacgaaaat gggggccaat gccagcttcc gcggtcggcc
     1741 gctagcccat caaagagagg gggagaatat atgatccgca ttctttctgt cgcagctgcc
     1801 atcgctgtca gtgctacggt cgtctacgca ggcgccgact tcatcgatca gcgccaacgg
     1861 gccatgaagg ctctggcgaa tgccgcgaag gcgcctggcg ccgtgctcaa aggggaagcc
     1921 aagttcgagc tcgataaggt ccatagctcg ctagcggtat tcaaagagca agccgccaag
     1981 ctcaaagatc tttggccgga cgactccaag gatggcgaga cgaacgccct tcccgccgta
     2041 tgggacaaga agagcgagtt tctggctcta ttcgacaagt ttgaggcaga cgtccaaacc
     2101 gcgtccgaaa agatcaagga cgaagactca ttcaaggccg agtggccgaa agttatgagc
     2161 aactgcggcg gttgccacaa acagtaccgc aagccccagt aatctaccga ctcgatttcg
     2221 tgctgccgcg tttggatcga cgccggcccg cgcccagcgg cctgctgcgg gctggtgcgt
     2281 cgatccactt ggcatgcaga ttcggcaaag actggcttgg ccgaaagcgg tgagagatga
     2341 tcatgaagcg acgctcgttc ctcacggtgc tactcaggct gctcgtcacc cccatcgtga
     2401 tagcaggtgc agccctttat ctgctcacga taccttcggt gctttcggag tcggagctgg
     2461 ctccccggac ggcggacctc gccaacggcg agaccatgtt ccatgctggt ggctgcgcgt
     2521 cgtgccatgc cacgccgggt gaggagggta agactcgtct cggcggcggc cttgctctca
     2581 acacggcctt cggcaccttc cgtgccccga acatctcgcc ggacccgcag tacggcatcg
     2641 gcagctggag cgagatcgag ttcgtgaatg ctatgctgcg cggtgtcggc cgtaatggcg
     2701 agcatctcta tccttcgttc ccctacacct cctacca
//
LOCUS       DASUMG010000304         2290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_682722, whole genome shotgun sequence.
ACCESSION   DASUMG010000304 DASUMG010000000
VERSION     DASUMG010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682722"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2058
                     /locus_tag="VFX46_05015"
     CDS             <1..2058
                     /locus_tag="VFX46_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEX5599971.1"
                     /translation="SAEALKAGLDGLFADGRPFNLLLKTNAGGHLEADGRASGARAVL
                     RLRDVAGYKRDLIKIIDQHRQLARDIRASRALLNALPMPVWIRGPDGRIEWVNKAYVA
                     AVEAADEAEVREKQIELLESRQRAALERKLNKREPLTTRMPLIIGGERKPHDIVIVPL
                     EDTTAAAAIDVTAVETAQGELDRQIAAYLRTLDRVATAVAIFGADRRLNFFNAAYQRL
                     WNLDRDWLETRPTDSDILDRLRETSHLPATVNYRAWKAKLLACYQSTTELEDWWHLPD
                     GRMLHVVAEQRPDGGIIQLFDDVTERFDLESQYNALINVQRETLDSLKEGVAVFATNG
                     RLKLCNQAFAAIWRLPGQVLEGQPHIDEIIRHACERFDNQRTWARISRAVTSISDQRE
                     QIEGQMVRPDGSVIDFTVAPLPDGATLITFADVTASKRIERALAERNEALVAADRLKS
                     QFISHVSYELRTPLTNIIGFTELLSSPRTGPLNEKQREYLSDITSSSKTLLSIIDDIL
                     DLATIEAGAMELKLARVDVRNVLKSAIQGIRERASRARLTLDVAVADDAVSFIADESR
                     VRQIIYNLLSNAVGFSKPGDTIRISCWRENGMMVFSVEDQGVGIPRDLQDRIFERFES
                     RSLGSKHRGVGLGLSISRHLVELHGGEMRLESEPGRGTKVTVRFPEVPAKLTVAEAMS
                     ARA"
     gene            2092..>2290
                     /locus_tag="VFX46_05020"
     CDS             2092..>2290
                     /locus_tag="VFX46_05020"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX5599972.1"
                     /translation="MTAPWIYARAREADVRHLAELLAFALKPGDVIALRGELGAGKST
                     LARALIRALLSDETAEVPSPTF"
BASE COUNT          425 a          750 c          709 g          406 t
ORIGIN      
        1 tctgctgagg cactgaaagc ggggctagac ggcctcttcg ccgacggccg gcccttcaat
       61 ctgctgctca agaccaatgc cggggggcac cttgaggcgg acggccgtgc ctctggcgct
      121 cgcgccgttc tgagattgcg cgatgtggcc ggctacaaac gcgacctcat caagatcatc
      181 gaccagcacc ggcagcttgc ccgcgacatc cgggcaagcc gcgcccttct caacgccctg
      241 ccgatgccgg tctggatcag agggcctgac gggcggatcg aatgggtgaa caaggcgtat
      301 gtggcagcag tggaagcggc cgatgaagcc gaggtgcgcg agaagcagat cgagcttctg
      361 gaatcccgcc agcgagccgc gcttgagcgg aagctcaaca aacgcgagcc gctcacgaca
      421 cgcatgccgt tgatcatcgg tggcgagcgc aagccgcacg acatcgtcat cgttccgctc
      481 gaggacacga ccgccgccgc cgcgatcgat gtcacggctg tcgagactgc ccagggcgag
      541 ctcgaccggc agattgccgc ctaccttcgc acgttggatc gcgttgccac cgcggtcgcg
      601 atcttcggag ccgaccggcg gctcaacttc ttcaatgctg cgtaccagcg cctttggaac
      661 cttgatcgcg actggctcga gacccgcccc acagacagcg acatcctcga tcgcttgcgc
      721 gagacctcac accttcccgc cacggtcaac tatcgtgcct ggaaggcgaa gctccttgcc
      781 tgctatcagt cgaccaccga actggaggat tggtggcacc tgccagacgg gcgcatgctc
      841 cacgtcgtgg ccgagcagcg gcctgacggc ggcatcatcc aattgttcga cgatgtgacc
      901 gagcgtttcg acctcgaaag tcagtacaac gccctgatca acgtgcagcg ggaaacgctc
      961 gacagtctca aggaaggcgt cgcagtcttc gcaacgaacg ggcggctcaa gctctgcaac
     1021 caagctttcg cagccatctg gaggctaccg ggccaggtgc tggagggcca gccacatatc
     1081 gacgagatca tccgccacgc ctgcgagcgg ttcgacaatc agcgcacttg ggcccgcatc
     1141 agccgcgcgg tgacatccat ctccgatcag cgcgagcaaa tcgaggggca aatggtgcgc
     1201 ccagacggca gcgtcatcga cttcaccgtg gcgcctttac ccgacggggc gacgctgatc
     1261 acatttgccg acgtgacggc ctccaagcgc atcgagcggg cgctcgccga gcgcaacgaa
     1321 gcgcttgtgg ccgctgaccg gctcaagagc cagttcatca gccatgtttc ttacgagctt
     1381 cgcacgccgc tgacgaacat catcggcttc accgagctgc tctccagccc cagaaccggc
     1441 ccgctcaacg agaagcagag ggaatacctg agtgacatca cgagctcttc gaagaccctg
     1501 ctgtcgatca tcgatgatat cctcgacctc gccaccatcg aggccggggc catggagctg
     1561 aagctcgcgc gggtcgacgt tcgcaatgtg ctcaagtccg ccattcaggg aattcgcgag
     1621 cgtgcaagcc gggcgcggct gacgctcgac gtcgccgtgg ccgacgacgc cgtttccttc
     1681 attgccgacg agagccgagt tcgtcagatc atctacaatc tgctgtcgaa tgcggtcggc
     1741 ttctccaagc cgggtgacac gatcaggatc tcgtgctggc gggaaaatgg catgatggtg
     1801 ttctcggtcg aggaccaagg tgtcgggatc ccgcgggatc tgcaggatcg catcttcgag
     1861 cggttcgaga gccggagcct tggctcgaag catcgcggtg ttggtctcgg gctttcgatc
     1921 tcgcgccacc tcgtcgagct gcacggaggc gaaatgcgcc tcgagtcgga gccgggtcgt
     1981 ggcaccaagg tcactgtccg gttcccggag gtcccggcca agctgacggt tgcggaggcc
     2041 atgtctgctc gggcttgacg atcgcccgcc cccggctttt cctctcgccg catgacggcg
     2101 ccctggatat atgctcgcgc gcgcgaggcc gatgttcgcc atttggcaga gctgctcgcg
     2161 tttgcgctca agccggggga cgtcatcgcg ctccgtggag agctcggcgc cggcaagtca
     2221 acgcttgctc gtgcactcat ccgtgctctg ctgtcggatg aaacggccga agttccgagc
     2281 cccaccttca
//
LOCUS       DASUMG010000305         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_682888, whole genome shotgun sequence.
ACCESSION   DASUMG010000305 DASUMG010000000
VERSION     DASUMG010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682888"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /gene="era"
                     /locus_tag="VFX46_05025"
     CDS             complement(<1..653)
                     /gene="era"
                     /locus_tag="VFX46_05025"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX5599973.1"
                     /translation="MADKEAVAGYGPQGETRCGFIAVIGAPNAGKSTLVNALVGAKVT
                     IVSRKVQTTRIPVRGIVVEGASQLVFLDTPGIFRPRRRLDRAMVEAAWAGALDADIVV
                     LMVDALKGITEDVERILDRLGEAHGLKVLVLNKVDRLVDKSKLLVLTDQLTKRIAFDR
                     VFMVSALTGSGLEDLKHYLVEVAPPGPWHYPPDELSDLSDRKLAAEITREKIYDRLH"
     gene            complement(646..1386)
                     /gene="rnc"
                     /locus_tag="VFX46_05030"
     CDS             complement(646..1386)
                     /gene="rnc"
                     /locus_tag="VFX46_05030"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX5599974.1"
                     /translation="MRRGTKRTELETRLGYTFKNRELFKRALTHASVRSHRGTRHDNE
                     RLEFVGDRVLGLAVADMLFELHPDASEGELARRFNRLVRGQACANVARRVGVGPYLIL
                     SESEAESGGREKETILAGALEALLGAVFIEAGFETARKIVRSLWSDLLDAMPEAIPDP
                     KSALQEWAQGQGLSLPEYIEISRSGPDHAPLFVTEVHIPGREPARGEGPTKRQAEQAA
                     ASVMLAREGVRLEALRRGDAGLKKSSHG"
     gene            complement(1393..2163)
                     /gene="lepB"
                     /locus_tag="VFX46_05035"
     CDS             complement(1393..2163)
                     /gene="lepB"
                     /locus_tag="VFX46_05035"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX5599975.1"
                     /translation="MSLNTDTGKKRESGWWETVKIVIQALALAMVVRVFFYQPFNIPS
                     GSMKSTLLVGDYLFVSKLSYGYSRYSFPFGLNLFSGRIFASPPQRGDVAVFKLPRDNS
                     TDYIKRVIGLPGDEIQMRNGVLYINGQAVPKVRVEDFVTREEDGVLRRIPQYEETLPN
                     GVKYRVLDSEPNGPFDNTGVFKVPENHYFVMGDNRDNSTDSRAQWGVGFVPFENFVGR
                     AEIIFFSAAFDDPDAFRLYTPWLWPRDIRWERFFTLVR"
BASE COUNT          393 a          723 c          648 g          400 t
ORIGIN      
        1 tcgtgcaggc ggtcgtagat cttttcgcgg gtgatctccg cagccagctt gcggtctgaa
       61 agatccgaca gctcatccgg gggatagtgc cacggcccag gcggcgcgac ctccaccaga
      121 tagtgcttca agtcctcgag cccgctgcca gtcagcgcgg agaccatgaa tacccggtcg
      181 aacgcgatcc gtttcgtcag ctggtccgtg aggacgagaa gcttgctctt gtctactagg
      241 cggtcgacct tgttcagcac aagcaccttc agcccgtgcg cctcgccgag ccggtcgaga
      301 atccgctcca cgtcctcggt gatccctttc aaggcatcga ccatcaggac gacgatgtcg
      361 gcatcaaggg caccggccca ggctgcctcc accattgcgc ggtcgagccg ccgtcggggc
      421 cggaaaatgc cgggggtgtc cagaaagacc agctggctcg cgccctcgac cacgatcccc
      481 cgcacgggga tgcgtgtcgt ctggaccttt cgcgaaacga tggtcacctt ggccccgacc
      541 agggcattga ccagcgtgga cttgccggca ttgggcgcgc cgatcaccgc aatgaagccg
      601 caccgcgtct cgccttgggg cccgtagccc gccacggcct ccttgtcagc catgggatga
      661 cttcttcagg ccggcatcgc cccggcgtag cgcctccagg cgaacgccct cgcgcgccag
      721 catcacgctg gccgccgctt gctctgcctg gcgtttcgtt ggcccctccc ctcgcgccgg
      781 ctcccggccc gggatgtgca cctccgtgac gaagagcggc gcatgatcgg gaccgctccg
      841 cgaaatctcg atgtactctg ggagcgagag cccttggccc tgcgcccact cctgcagcgc
      901 tgacttcgga tcgggtatcg cttcgggcat ggcatcgagc agatcgctcc acagcgatct
      961 cacaatctta cgcgcagtct cgaaaccggc ttcgatgaac accgctccca ggagcgcctc
     1021 gagagcgccg gccagaatcg tctccttctc gcgcccgccg ctctccgcct cgctttcgct
     1081 gaggatcagg tacggcccca ccccaaccct ccgcgcgacg ttggcacagg cttgcccgcg
     1141 gaccagccgg ttgaagcggc gggcaagctc tccttcgctg gcgtcggggt gcagctcgaa
     1201 gagcatatcg gcaacggcga gccccaacac tcggtcgccg acgaattcga gccgctcgtt
     1261 gtcgtgccgc gtcccccgat gcgaccggac gcttgcatgt gtcaaagcgc gtttgaacag
     1321 ctcacggttc ttgaacgtgt agcccagtcg cgtctcgagt tccgtcctct tcgttccgcg
     1381 tcgcacgtct gttcagcgca ccaaggtgaa gaaccgctcc cagcgaatat cccggggcca
     1441 gagccagggc gtgtagagcc ggaatgcgtc gggatcgtcg aaggccgccg agaagaagat
     1501 gatctccgca cggccaacga agttctcgaa tggcacgaag cctacacccc actgcgcacg
     1561 gctgtccgta gagttatcgc ggttgtcccc cataacgaaa tagtggttct cggggacctt
     1621 gaacacgccc gtgttgtcga acggaccgtt gggctcggaa tcgagcactc tgtatttcac
     1681 gccgttgggc agcgtttcct catactgcgg gatgcgacgc aacacgccgt cttcctcgcg
     1741 ggtgacgaaa tcctcgacgc gaactttggg aacggcctgg ccattgatgt agaggacacc
     1801 gttccgcatc tggatctcgt cgcccggcag gccgatcacg cgcttgatgt agtccgtcga
     1861 gttgtcacgc ggcagcttga acacggcgac atcgccccgc tgaggaggcg acgcgaaaat
     1921 gcgaccggaa aacagattga gtccaaatgg aaacgagtag cggctgtagc cgtaggacag
     1981 cttcgaaacg aacaggtagt cgcccacgag cagcgtcgac ttcatcgagc ccgacggaat
     2041 gttgaagggc tggtaaaaga acacgcgcac taccatggcg agcgcgagcg cctgaatgac
     2101 gatcttgacg gtttcccacc agccgctttc gcgcttcttg ccggtgtctg tgttcagact
     2161 catt
//
LOCUS       DASUMG010000306         3226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_441610, whole genome shotgun sequence.
ACCESSION   DASUMG010000306 DASUMG010000000
VERSION     DASUMG010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441610"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2829)
                     /locus_tag="VFX46_05040"
     CDS             complement(<1..2829)
                     /locus_tag="VFX46_05040"
                     /inference="COORDINATES: protein motif:HMM:NF037694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX5599976.1"
                     /translation="MTKLLRLVFFALVLWLPAQQSSAQTNTFAHPEIAREAQRYENEL
                     ATKTAAGSRPRQDLIAHGRRALNAGDVRAAASAFAQAAVLAPENAETWLLLARALLAT
                     SPDPNHPSERYELPANASAAAYLAYKRATSPAMQAQALAVLGRALQYRSVWRPALNAL
                     KISLSISEDPEVRQEYERLRAEHGFRLVDYTVEQDVAQPRVCLQFSENILRGQDLTKF
                     IAVDGKDPQAIAVEGSQLCVEGLSHGQRYQVLVRAGLPSEVDESLQQPAELSIYVRDR
                     SPFVRFTGRSYVLPSRGQAGIPVVSVNTNKVAVEIYRIGDRSHVSAVVNGDLRRQLSR
                     WDLHEMRETKGVRVFTGTLDVPMRLNEEVTTALPVGEAVPQLEPGAYALVAKPTESKD
                     EYGPFATQWFIVSDLGLTTFTGDDGIHAFVRSLATTAPVANVAVRLVARNNEVLGQGR
                     TDTAGHVHFERGLTRGEGGLAPALLIAEGDGGDYAFLDLTTSAFDLSDRGVKGREAPG
                     PLDAFLFTERGVYRPGEDVHLTALVRDRAGNASGVPVTLIVTRPDGVEHTRVPLTQDE
                     LGGHTTTLRLAPSSMTGTWRARLHADPLDDPLTSVAFLVEDFLPERLDMTIDQVAEAL
                     RPGQSGVVKVSGRYLYGAPAAGLAIEGDIVVRPAAGGLAAYPDYQFGLADDTVSPVRN
                     AIDGLPSTDSEGRAELQVMLPPVPRTARPLEAELFLRLREPSGRAIERSVTLPVEVQL
                     PRIGLKPLFRDGRVGEGTTAEFDVIHVGSSGERARLAGAKWQLVRLEQRWQWYNRDGY
                     WAYEPITLTQKVASGVIDIDAEKPARIAAQVDWGRYRLEVASADTGGPASSTIFTAGW
                     YSEEGTDSPEALEIALDKPSYNVGDVARLKIEDRYGGRAFVAVLGDGLLASQEVELPK
                     GGGELTIPVERDWGPGAYVTAMLYRPMDE"
     gene            complement(2988..>3226)
                     /locus_tag="VFX46_05045"
     CDS             complement(2988..>3226)
                     /locus_tag="VFX46_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599977.1"
                     /translation="DSMRRDPLSHDACELYAPDHPETAQRASLFFERLWQATEPVLER
                     SSPAMDDEVGPHLIHPVGLISPKSGPKLGPRTSA"
BASE COUNT          590 a         1075 c          993 g          568 t
ORIGIN      
        1 ctcgtccatc gggcggtaaa gcatcgccgt gacgtaggcg ccgggccccc agtcgcgctc
       61 gacgggaatc gtcagctcac cgcctccttt cggcagctcg acttcctggc tcgctagcaa
      121 gccatcaccc agaaccgcca cgaaggcgcg gccaccgtac cgatcctcga tcttgaggcg
      181 ggcgacgtca cctacattgt agctcggctt gtcgagggcg atctcaagag cctctggact
      241 gtccgtccct tcctcggagt accatccggc cgtgaaaatg gtgctggaag ctggaccgcc
      301 cgtgtcggca gacgcgacct ccagacgata ccggccccag tcgacctgag ccgcgatgcg
      361 cgcgggtttc tcggcatcaa tgtcgatgac gccgctcgcc accttctgag tgagcgtgat
      421 cggctcatag gcccaatagc cgtcgcggtt gtaccactgc catctctgct cgagccgcac
      481 cagctgccac ttggcacccg ccagccgcgc ccgctcaccg ctgctgccga catggatgac
      541 gtcgaattcg gctgttgtcc cctcgccgac ccggccatcg cggaacagtg gcttcagtcc
      601 gatccgcggc agctggacct cgacaggcag cgtgacgctt cgctcgatcg cacggccact
      661 cggctctctg agcctcagga acagttcagc ctccagcggc cgcgctgtcc gcggaaccgg
      721 cggcagcatg acttgaagct ccgcgcggcc ttcggagtcc gtgctcggaa gcccgtcgat
      781 ggcattgcgc acggggctga ctgtgtcgtc cgcgaggccg aattgatagt ctgggtaggc
      841 cgcgagacca cccgccgccg gcctcacgac gatgtcgccc tcgatggcga gaccagcggc
      901 cggcgcgcca tagagatagc gccccgaaac tttgacgacg cctgactgcc ccggcctgag
      961 ggcctccgcg acctgatcaa tcgtcatatc gaggcgctcg ggaaggaaat cctcgacaag
     1021 gaaggctacg ctggtcagcg ggtcgtccaa aggatcggcg tgcagccgcg cgcgccacgt
     1081 ccccgtcatg ctcgacggcg ccagccgaag cgtcgttgta tgaccaccga gctcgtcctg
     1141 cgttaacggt acccgcgtgt gctcgacacc atccggcctg gtgacgatga gagtgaccgg
     1201 gacgccggaa gcattaccag cccgatctcg aaccagagcg gtcaggtgca catcctcgcc
     1261 tggccgataa acgccacgct ccgtgaacag aaatgcatcc agcggaccgg gggcttcccg
     1321 ccccttcacc ccacgatcgg acaaatcgaa tgccgacgtc gtgaggtcga gaaaggcata
     1381 atcgccgccg tcaccttccg cgatgaggag cgccggtgcc agaccgccct ccccgcgcgt
     1441 caggccgcgc tcgaagtgaa cgtggcccgc tgtatccgtc cgcccctgcc cgaggacctc
     1501 attgtttcgc gccaccagtc gcacggcgac gttcgcaacg ggcgcagtgg ttgcgagcga
     1561 ccggacgaaa gcatggatgc catcgtcccc ggtgaaggtg gtgaggccaa ggtccgagac
     1621 aatgaaccac tgcgtcgcaa acggtccgta ctcgtctttg gactcggtcg gttttgcgac
     1681 cagagcgtag gcgccaggct ccagctgggg caccgcctct ccgaccggca gcgcagtcgt
     1741 cacctcctca ttgagacgca tggggacgtc gagcgtgccc gtgaaaaccc gcacgccctt
     1801 ggtctcgcgc atctcgtgga ggtcccagcg cgaaagctgt cgtctaaggt caccgttgac
     1861 gacggctgag acatggctgc ggtccccaat ccggtagatc tcgacagcaa ccttgttggt
     1921 gttgacggag acgaccggga tgcctgcttg gccgcggctc ggcagcacat agctgcgccc
     1981 ggtgaagcgc acgaacggcg accggtcgcg aacgtaaatg gaaagctccg ccggctgctg
     2041 cagggactca tcaacctccg acggcaaccc cgcccgcacg agcacctgat atcgctgccc
     2101 gtgggaaagg ccctccacgc aaagctgcga accttcgacc gcaatggctt gcggatcctt
     2161 cccgtcgact gcaatgaatt tcgtcagatc ctgaccgcgc aggatgttct cggagaattg
     2221 aaggcaaacc ctcggctggg cgacgtcctg ctcgacggtg tagtcgacaa ggcggaagcc
     2281 gtgctcggcc ctcaacctct cgtactcctg ccgcacctcc ggatcttcgg agatcgaaag
     2341 gctgatcttc aaagcattca aagccggccg ccagaccgat cggtattgga gagcacgacc
     2401 gagcactgcc agcgcctgcg cttgcatggc cgggctcgta gcacgcttgt aagcaaggta
     2461 cgccgcggcg ctcgcattgg cgggcagctc gtaacgctcg ctgggatgat tgggatcggg
     2521 agatgtggcc aggagggctc gcgcgagcag cagccatgtc tccgcgttct ccggggcaag
     2581 tacagcagcc tgagcgaaag cgctggccgc tgccctcaca tctcccgcat tcagcgccct
     2641 cctcccgtga gcgatcaggt cttgtcgcgg gcgcgatcct gctgccgtct tggtcgccag
     2701 ctcgttctcg tagcgctggg cctctctggc gatctccgga tgcgcgaaag tattggtttg
     2761 agcactactt tgttgtgccg gaagccaaag aacgagggca aagaatacga ggcgcaggag
     2821 tttggtcatg gcacatccct cgcgagcaga tccgcccagg ccaagcttta cggcactagg
     2881 ggcctgacct tacactcgct caagttgccc tgaccgaaca aggggccttc tgagagatac
     2941 cccttgatcc aatcaacttg cgccagttgc cgctcgttgg cccggactta agcagatgtg
     3001 cgtggaccga gcttgggacc ggattttggc gagatcaatc cgacggggtg gatgagatgc
     3061 ggaccaacct cgtcatccat tgctggcgac gaacgctcga gcacgggctc ggttgcctgc
     3121 cagaggcgct caaagaacaa gcttgcgcgc tgtgccgtct cgggatggtc gggtgcgtaa
     3181 agctcgcagg cgtcgtggct cagcggatct cgccgcatgc tgtcac
//
LOCUS       DASUMG010000307         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_602469, whole genome shotgun sequence.
ACCESSION   DASUMG010000307 DASUMG010000000
VERSION     DASUMG010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602469"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VFX46_05050"
     CDS             <1..676
                     /locus_tag="VFX46_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX5599978.1"
                     /translation="RKPSARTNAPKLSMQPAPDKSRVAALLLAAGSSRRFGGGNKLLA
                     DVDGRPLIYWSAAAFVSSRASELVVVTGPEPEVIEAALTGLPAQFVHNPDHLSGMGGS
                     VAVGISALGDNCTGVLICPGDMPGVSTDLINALIAAFEATGSSRIIRPMLSGERPGNP
                     VLWPRRYFPDLARLKGPAGGRTLLADLNKEIEYLPWQDQGAALDIDTPEDLERYRQAK
                     HRSHSS"
     gene            752..>2087
                     /locus_tag="VFX46_05055"
     CDS             752..>2087
                     /locus_tag="VFX46_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX5599979.1"
                     /translation="MRTGELEKPTPKQRPRLVLFGYELALWRAAVVLLAVMLAAGIAL
                     TPAPQGVAQKAMVALGLISMTVVLWATLAIPPPLAAAIFIGLVIVTGAAAPASAISGF
                     LSSSLWLVFGGLMIGAAAERTGFGRFIARRFLGRFRSSFSALVLGVIAGSTLLTFLVP
                     SNIGRLAITIPVVLALADDAGYRLGSNGHVGLVLIAVVGNFAVGQGVLPGNLLNMMII
                     GAGETLYGLDVSYMHYLLLCAPVLGLAKGLLVWRLLVWMYPAPAPRPNGEMRDDRLGP
                     EAKRVGVILAAAILLWATDFWHGIKPGWVALAAGALCVVPGIGVLPPRELVNPQRLLI
                     IVWVGTVLSLAAVMTESGAGELVSSALAQLAGVEGRSPFYGYFAMAYLSSVIATLATF
                     GGGIPIAAATAAEVSAATGLPLETAVMALAPGMSAMFFPYIASPIVVGLAMGR"
BASE COUNT          332 a          646 c          667 g          442 t
ORIGIN      
        1 ccgaaagccg agcgcaagaa ccaacgcgcc gaagctcagt atgcagccag cgcctgacaa
       61 gagccgcgta gcagccctgc tgttggcggc tggctcatcg cggcggtttg gcgggggcaa
      121 taaactgttg gcagatgtag atggtcgacc gctgatttat tggtccgccg ccgccttcgt
      181 gtctagccgg gcttcggagc tggtcgtcgt gacgggcccg gagccggaag tcatcgaggc
      241 tgcactcacc ggactgccag cgcaatttgt gcacaatccg gatcacctgt ccggcatggg
      301 cggatcggta gccgtcggga ttagcgcgtt gggggacaat tgcaccggtg tcctgatttg
      361 cccgggagac atgccgggtg tctcgacgga tctcatcaac gcactaatcg ctgcgttcga
      421 ggccaccggc tcaagccgca tcattcgccc gatgctgtcc ggagaacgac caggtaatcc
      481 cgtactgtgg ccgcgccgct atttccctga tttggctcgt ctcaaagggc cagcgggcgg
      541 gagaacgcta ctggcggatc tcaataagga aatcgaatac ctgccctggc aggaccaagg
      601 cgccgccctg gacatcgata cgcccgagga tctcgagcgg tatcgtcaag ccaagcatcg
      661 atcgcatagc agctaggcat tcctggcctt gacgcgaaat gcgtgaccct gattgcctca
      721 ccgcggtgtt tggagctggg gcacacgcga gatgaggacg ggcgagctgg aaaaaccaac
      781 accgaaacag cgccctcggc ttgttctctt tggatacgag cttgcactct ggcgggcggc
      841 ggtcgtcttg ttggcggtca tgctcgccgc aggaatcgct ctaacgcctg cgccgcaagg
      901 cgttgctcaa aaggcgatgg tggcgctcgg cctgatttcc atgacggtcg ttctgtgggc
      961 gacccttgcg attccaccac ctctagcagc tgcaattttc atcggtttgg tcatcgtgac
     1021 gggggcggca gctcccgcga gcgcgatttc aggcttcctt tcgagctcgc tgtggttggt
     1081 gttcggtggc ctgatgatcg gggccgctgc tgagcgtacc ggcttcggcc ggttcattgc
     1141 ccgaagattt ctcggaagat ttcgcagctc attttctgcc ctggtgctcg gtgtcattgc
     1201 cggctcgacg ttgctgacat ttctcgtgcc ctccaatatc ggccggctgg caattacaat
     1261 tcccgttgtt ctcgcgctgg ccgatgacgc cggctatcga ctgggctcca acggccatgt
     1321 cggcctcgtg ctgatcgcgg tcgtggggaa ttttgccgtg ggccagggcg tgctccccgg
     1381 caacctcctc aacatgatga tcatcggtgc cggcgaaacg ctgtacggcc tcgacgtcag
     1441 ctacatgcac tacctgcttt tgtgcgcgcc ggtcctgggc ctcgccaagg gattgctcgt
     1501 ctggcgtctt ctcgtgtgga tgtacccggc ccctgctccg cgccctaacg gtgagatgcg
     1561 agacgaccgg cttggccctg aggcaaaacg cgttggcgtt atcctcgcag ctgcgatcct
     1621 tctctgggca accgacttct ggcacggcat caagcctggg tgggtggcgc tggcagccgg
     1681 tgcgctttgc gtcgtgcctg gcatcggggt cctgcctccg cgggagctcg tcaacccgca
     1741 gcgcctgctc atcatcgtct gggtcggcac ggtgttgagc ctcgccgcgg taatgacgga
     1801 gtcgggagca ggcgagctgg tatccagcgc actggcgcag ctcgccgggg tcgaaggccg
     1861 gtcgccgttc tacggctatt tcgcgatggc ctatctttcg tcggtgatcg ccacactcgc
     1921 gacgttcggc ggcggcattc cgattgccgc agccaccgca gccgaggtca gcgccgccac
     1981 cggcctgcca ctcgaaacgg ccgtgatggc actagcgccc ggaatgtctg cgatgttctt
     2041 cccgtacatc gcatccccga ttgtggtcgg cctcgcgatg gggcggg
//
LOCUS       DASUMG010000308         3579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_121524, whole genome shotgun sequence.
ACCESSION   DASUMG010000308 DASUMG010000000
VERSION     DASUMG010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121524"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VFX46_05060"
     CDS             complement(<1..337)
                     /locus_tag="VFX46_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX5599980.1"
                     /translation="MVIRKIITAIVTVLVGLLPIAAVAAPGYGSSLLWGGTAEWGGGR
                     QSVPFSTAYKPGEIVVSFGDRRLYYITKPGQAISYPIAIPREESRWQGVTTVSMKKEN
                     PSWTPTPSML"
     gene            687..1697
                     /locus_tag="VFX46_05065"
     CDS             687..1697
                     /locus_tag="VFX46_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599981.1"
                     /translation="MAQVPAKPCLSLNAPSDTLPGAERLGEPEDVAARLRRRNLESLR
                     QRIRRIERSAGTGLEGPTLRPPIYDYSLGKTSGAEEHVPTNPLSGKIAGPGLTWQLGV
                     AEVDGLLGPEGLEIGGVHEIKPALQEQGTDWAATWATAFIFSLALAIRRCAMPDGRAC
                     RPILCCRPAAMASEFGGPYGPGLRALGLDPKRLIIVETKTAADTLWAIEEGLKSRGLA
                     LVLGVLGEIELTPARRLALAASAYCAPCVLLTHPRTETMAATATRWRVGPLPSGPHPF
                     DPHAPGAPRFTVDLERCRAQPLSAETASFSLEWCDEALCFRLAPGVADRADAARKSGR
                     GA"
     gene            1618..3243
                     /locus_tag="VFX46_05070"
     CDS             1618..3243
                     /locus_tag="VFX46_05070"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HEX5599982.1"
                     /translation="MRRFVFVWLPAWPIERMRRANPDAVPDDRPFALVEAGSHGIVIA
                     AANAQARAEGVRPGQSLADARAIYPGLLTRKIERSKDRAALFALARWSGRYGPARNVD
                     GESGFWIDITGVAHLFGGERQLMADLVSRLRAFGLTVRAGLADRIAAAAALARFARVG
                     TEGFAIAPAGRMQEALRDLPVEALGLADDTILLLKRLGLRRIGQLYDLPRPALARRFR
                     SLAPGRRKVKNSTELAGAVLARLDAALASAPEARHALPETRRAIVRRIFSEPLISSEG
                     IESAVADLAASLCRMLDQAGRGARRVRLCFYRVDGTVAEVRAGTSRPCREPKHLLQLL
                     APKLPEIDAGFGVDVLTLEATALELLDPLQGRLSAGLEEWTEADTAQLIDRLSNRLGA
                     ERVFRLVPYESHIPERAEVRIPALMAKDPREKQGAERSKVSHISTTSLRPPLLLAIPE
                     PITVLAEVPEGAPARFTWRRVTHTVVKAEGPERIEPEWWRMLGPLARPPGPRDYYRIE
                     DDRGGRYWVFREGLYDRDPEAGPPVWYMHGLFG"
     gene            3254..>3579
                     /locus_tag="VFX46_05075"
     CDS             3254..>3579
                     /locus_tag="VFX46_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX5599983.1"
                     /translation="MFSAPAYAELQVTTNFSFLRGGSHPHELVAQAKALGLSALAVTD
                     RNTLAGVVRAHTAAKEAGLKLIVGARLDLQDAPSLLCLPTNRAAYGRLCRLLTLGQRR
                     AEKGQCT"
BASE COUNT          623 a         1155 c         1145 g          656 t
ORIGIN      
        1 ggagcatcga cggagtcggc gtccacgacg ggttttcttt cttcatagag acagtcgtta
       61 cgccctgcca gcggctttcc tcgcgcggaa tagcgatcgg atagctaatg gcctgtccgg
      121 gtttggtaat gtagtaaagc cgccggtcgc cgaagctgac tacgatctcc ccgggtttgt
      181 aagccgtgct gaacgggacg gattgacgac cgccgcccca ttcggcggtg cctccccaaa
      241 gcagactgga tccatagccc ggcgctgcca cggcagctat gggaagaagg ccgacgagta
      301 cagtaacaat cgcagtgatg atcttacgta tgaccatctc gtactccgtt aaggctcaga
      361 tgatcgagcc gatgggccgc tcgccccacc gggtacatca tcttagtcaa gacgtgatga
      421 atggttcata tacagcagct tcggtccgaa aaccggcaca tgaaagccac actctggcca
      481 agattttggg cagtgggctg attctcggat gaggaggcgg gggcattcgg tgccggaggg
      541 cgggttcgct cacgaactgc agtgaacttg acctacgtat ctgagttggc cctcgcacga
      601 gcgaggcgca tgccagggtt gaccaggggc gtgcaatggc gtaaagaaca taacgagaac
      661 aaaaatgaag ctcaatggca gcaggcatgg ctcaagttcc tgccaaacct tgcctttctc
      721 tcaacgcccc gtccgatacc cttccgggtg ccgagcggct aggtgaacct gaggatgtcg
      781 cggcgcgcct gcggaggcgg aacctggaaa gccttcggca gcggatccgc agaatagagc
      841 gtagcgccgg cacagggctg gaagggccaa ctctgcggcc tcccatttac gattacagtc
      901 taggcaaaac ttcgggcgcg gaggaacacg tgcccaccaa tcctctctcg ggcaagattg
      961 ccggtcctgg tctgacgtgg cagctcggcg ttgccgaagt cgatggtctc ctggggcccg
     1021 aagggctcga gattggcgga gtgcatgaga tcaagcccgc gctgcaggaa caggggacag
     1081 actgggctgc aacttgggct acggcattca tcttctccct cgctctggcc atccgccgct
     1141 gcgccatgcc agacgggaga gcgtgcaggc caatcctctg ctgccggccg gccgcaatgg
     1201 ccagtgagtt tggcggccct tatggtccgg ggttgcgggc gctgggtctc gacccgaaac
     1261 gcctcatcat cgtcgaaacg aaaacggcgg ctgacaccct ctgggccatc gaggaagggc
     1321 tgaaatcgcg aggtctggcg ctcgtccttg gtgtcctggg ggagatagag ctgacgccgg
     1381 cccggcgtct ggcattggcg gcctccgctt attgcgcgcc ttgcgtcctc ctcacccatc
     1441 cgcgaaccga gacaatggcc gctaccgcaa cccgctggcg cgtggggcct ctgcccagcg
     1501 gcccgcatcc cttcgatccg cacgcccccg gtgctccccg gttcacagtc gatctcgaac
     1561 gctgccgggc gcagccgctt tcggccgaga ctgcctcttt cagtttggag tggtgtgatg
     1621 aggcgctttg ttttcgtctg gctcccggcg tggccgatcg agcggatgcg gcgcgcaaat
     1681 ccggacgcgg tgcctgatga ccgtccgttc gcactcgtcg aagcaggttc gcatggcatc
     1741 gtcattgctg ctgcgaatgc ccaggcccgc gccgagggtg ttcggcccgg ccagtctcta
     1801 gccgatgcca gggcaattta tccaggcctc ctgacgcgca agatcgagcg aagcaaggat
     1861 cgggcggcgc ttttcgcctt ggcgcgctgg agcgggcgct acggtccagc ccgaaatgtc
     1921 gatggggaga gcggattttg gatcgacatc accggcgtgg cgcacctctt cggaggcgag
     1981 cgccagttga tggccgatct cgtcagccga ctgcgtgcct tcgggctgac cgtgcgggcc
     2041 ggccttgccg atagaatcgc cgcagctgcg gcgctcgccc ggtttgcccg cgtgggaacg
     2101 gaaggatttg ccattgcgcc cgccggccgc atgcaggaag cgctccgtga tctgcccgtc
     2161 gaagcactgg gtttggcgga cgacaccatt ctcctgctaa agcggctcgg gctgcgccgt
     2221 atcggccagt tgtacgacct gccccgcccg gccttggcgc gccgttttcg atcgctcgca
     2281 cctgggcgcc gcaaagtaaa gaactccaca gaacttgccg gcgcggtgct ggctcgcctc
     2341 gatgccgccc tcgcctcagc acccgaagcg aggcatgcgc ttccggaaac tcggcgagcc
     2401 atcgtccgcc gcatcttctc agagccgctg atttcctcgg aagggatcga aagcgccgtc
     2461 gccgatctcg ctgcgagcct gtgccggatg ctcgatcaag ccgggagagg cgcccgtcgc
     2521 gtgcggcttt gtttctatcg cgtcgacggg accgtggcag aggtccgggc tggcaccagt
     2581 agaccctgcc gtgagcccaa acatctcctg caactgctgg cgccgaagct cccggaaatc
     2641 gatgccggtt tcggtgtcga tgtgctgacg ctggaagcca cggccctcga actgctcgat
     2701 cccctccagg ggagattgag cgccgggctg gaggaatgga cggaagccga caccgcccag
     2761 ctcatcgaca ggctctccaa ccggctcggg gcggagcgcg tgttccggct tgttccttat
     2821 gagagccata tccccgagcg cgccgaggtg cgcattcccg ccctaatggc gaaagacccg
     2881 cgtgaaaagc aaggggcgga gcgctccaaa gtctcgcata tttcgactac ttcgctccgc
     2941 ccaccgctcc tgctcgccat cccggaaccg atcacagtgc tcgccgaagt gcccgaaggc
     3001 gcgccggcgc gtttcacatg gcgccgcgtc acccacaccg tggtcaaggc cgaagggccg
     3061 gagcgcatcg agcccgaatg gtggcgcatg ctcggccccc tcgccagacc gccgggtccg
     3121 cgggactatt accgcatcga ggatgatcgc ggcggccgat actgggtgtt tcgcgaaggc
     3181 ctttatgacc gcgatcccga agccgggccg ccggtctggt acatgcacgg attgttcggg
     3241 tgaggagcat ctcatgttct ccgctcccgc ctacgccgag ctgcaggtca cgacgaactt
     3301 ctcgtttctg cgcggcggct cacatccgca cgagctggtc gcccaggcca aggcgcttgg
     3361 cctttccgcg ctcgctgtaa cggatcgcaa tacgctcgcc ggtgtagtgc gggcgcacac
     3421 ggctgcgaag gaagccgggc tgaagctaat cgtcggcgcc cgcctcgacc tgcaggatgc
     3481 gccgagcctg ctttgcctgc ccacgaaccg cgccgcctac ggccgcctct gccgtctcct
     3541 cactctcggt cagcggcggg cggaaaaagg gcagtgcac
//
LOCUS       DASUMG010000309         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1165319, whole genome shotgun sequence.
ACCESSION   DASUMG010000309 DASUMG010000000
VERSION     DASUMG010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165319"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFX46_05080"
     CDS             complement(<1..680)
                     /locus_tag="VFX46_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5599984.1"
                     /translation="MKAGSVAAAFRTVGLALILAVMASAGAEAAEVTLRLKGGGFQLT
                     GELKEYDTTKYVIENRSFGTMTLDASRFDCIGQGCPRAPFAAPAALNLARGTSRTIRI
                     HGSDVVGAELMPLLVQGYANSIGAKAVRAVGANPLEMQIRLTDANDREITLVELHRYG
                     TDQAFQGLANGSVEIVMADRPVTEDEVAMLARAGLTDIRSPAHEHVIALDGLAIVVSE
                     DNPAVSLS"
     gene            complement(706..2124)
                     /locus_tag="VFX46_05085"
     CDS             complement(706..2124)
                     /locus_tag="VFX46_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599985.1"
                     /translation="MRPVIRLLPVVVLLPVISAGTPTAAPGFSPAEPAHQPLALPVPS
                     RGPSAESYEVLRLAQNAPVLHVARVFVMEPGTEIPLPIQVEAMPRNSFLRLRGLPSNA
                     TLSEGHQIGPGAWAVPLVSLPNIRVRVPRESTGRFEVSISAVNVAGDILSEIKTTLVV
                     ASSSSLLAGLSEQPAETPEPTLPQPPAPTLAERTDAGQPSATDTSASGMGGQPPLSSA
                     SAANIQPPSSKSCGAAEISTAALPAAQMAISIKATDCRANEPVTIEYAGARFVRQLDE
                     AGELSFTLDCFAGTSSKVVISFADGARGAIDVTARDLGRVSKVAVIWKAPVNLDLHAF
                     EYAASYGEPGHVWAASPSSPNDAWEKTLATGRGHGFITAADGNTEGDKYEVYTFWHHE
                     EQTSGAIEMAVDFESRGDTPSGDMCGNGPLSQVAFEVVMLSRHGEVTRQQAMMLPMEC
                     GATLTQSARYNKSVIPVLRVRR"
BASE COUNT          474 a          823 c          830 g          508 t
ORIGIN      
        1 atcgagagcg ataccgccgg gttgtcctcg gaaacgacga tggcgagacc gtccagtgca
       61 atgacatgct catgtgcggg ggagcggata tcagtcaggc cagctcttgc cagcatggcg
      121 acctcgtcct ccgtaacagg ccggtcggcc atgacgattt ccacggagcc gttcgccaga
      181 ccctgaaacg cctgatccgt tccatagcgg tgcaattcca ccagggttat ttcccggtcg
      241 ttcgcgtccg tcagtctgat ctgcatctcg agggggtttg ctcctacggc cctgaccgcc
      301 ttggcgccga tgctgttggc gtagccttga acgagcaggg gcatcagctc ggcgccgacc
      361 acgtccgagc cgtggatccg gatcgtccgt gaggtgccgc gggccaggtt gagagcggcg
      421 ggagcggcaa acggcgcgcg gggacagcct tgaccgatgc agtcgaacct cgacgcatcg
      481 agggtcatcg tgccgaagct tctgttctcg atcacgtatt tggtcgtatc gtactccttg
      541 agctcgccgg tgagctgaaa tccgccgcct ttgaggcgaa gcgtcacctc ggctgcctcg
      601 gccccggcgc tcgccatcac ggcgaggatc agcgccagac caacggtgcg aaacgctgcc
      661 gccacactgc ctgctttcat tgagcttcgt ccccccgatg ctcagtcatc gccggacccg
      721 taagacgggg atgaccgatt tattgtagcg cgccgactgc gtgagggtgg cgccgcattc
      781 catgggcaac atcatcgcct gctgtcgcgt cacctcccca tggcgtgaca gcatcacgac
      841 ttcgaacgcc acttgggaca gcggtccgtt tccgcacatg tctccgcttg gcgtgtcgcc
      901 gcgtgattcg aagtcgactg ccatctcgat ggcacctgag gtttgctcct catgatgcca
      961 gaaggtgtag acctcgtact tgtcgccttc ggtattccca tccgcagcgg tgatgaagcc
     1021 gtgcccgcgg ccggtggcca gcgtcttctc ccacgcgtca ttcggcgagg atggcgatgc
     1081 ggcccacacg tgccccggct cgccgtagga ggctgcgtac tcgaaggcgt ggagatcgag
     1141 gttcaccggg gctttccaaa tgacggcaac tttcgagacc cgtcccaggt cgcgggcggt
     1201 cacgtcgatg gcaccgcgag cgccgtctgc aaacgagatc acaaccttcg agctggttcc
     1261 cgcgaagcag tccagcgtga agctcagctc gcccgcttcg tcgagttggc gcacaaatct
     1321 tgcgcctgcg tactcgatcg tcaccggctc gttggcgcgg caatccgttg ccttgatact
     1381 aatggccatc tgagccgcag gcagagcagc cgttgagatc tcagcggcgc cacaagattt
     1441 cgatgatggg ggctggatgt tggcggcgct cgcactcgag agtggcggct gtccgcccat
     1501 tccacttgcc gaggtatccg ttgcactcgg ctgccccgcg tcagtccgct ctgccaacgt
     1561 gggcgcaggc ggctgtggca aggttggttc gggtgtttcc gccggctgct ccgataatcc
     1621 agccagcaga gatgacgagg aggcaaccac caatgtggtc ttgatttcgg acaggatgtc
     1681 ccccgccacg ttcaccgcac tgatgctgac ctcgaaccgg cccgtcgact cgcgtggaac
     1741 gcggacacgt atgttgggga gtgacacgag cgggacggcc catgcacctg ggccgatctg
     1801 atggccttcc gacaacgtcg cgttcgatgg caggccgcgc agccggagga agctgttgcg
     1861 cggcatggcc tccacctgga tgggaagcgg gatctccgtt cccggctcca tcacgaacac
     1921 acgcgcaacg tgaagcacag gagcattctg tgcgagacgc aaaacttcat agctttcggc
     1981 gctagggccg cgcgaaggga caggcagggc cagtggctga tgagctggct cggcaggact
     2041 aaagccgggt gctgccgtcg gtgtgccagc cgatatgacc ggcagtagca cgacgaccgg
     2101 caagagccgg attacaggcc gcatccggat aaacccacgg cgacccgagg cagggtggag
     2161 ttcgccccgc cttggagcgc tgagcttgct gggatggcaa tgctccgtcg gcattgtcgg
     2221 acccctctat gcgctgacgc tcccattctt gacgaggaga gcaggcattc gggccaggtc
     2281 gtcggagctt ttgtgggaga tccggtagtc agatgacagg tgctgaggcg ggcgcctgcc
     2341 cggacgaagg cgacataaca taacgcaccc cctagtcgac gtttcccccg agcggctact
     2401 tctttgtcag ccgtctcgca tcgcgatcct ttgacggatc agtcctaaga caatgaacag
     2461 catttctgcc tgttggtaaa ggtccgaact cgacctttct ttggcttcgc gtgagcaaaa
     2521 accacgaagg cgagatcaac tctccgcagc cggcccgagg tggcgctgcg caagggcgat
     2581 tgggtcgctg tcgcggcgag agcgaccttc aaaataacag cccttctcga gcacg
//
LOCUS       DASUMG010000310         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1004904, whole genome shotgun sequence.
ACCESSION   DASUMG010000310 DASUMG010000000
VERSION     DASUMG010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004904"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFX46_05090"
     CDS             <1..394
                     /locus_tag="VFX46_05090"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX5599986.1"
                     /translation="MRNASLKFVCKEVWLRIEQPDTIFTSRFKRGEVMPAIAAHAEGN
                     YFADADTLDRLESEGRVVFRYADPQGNVTPEANPNGSQRSIAGICDERRRVLGLMPHP
                     ENASDPLLGPTGGRRLFESLVDNLVGAA"
     gene            complement(525..1001)
                     /gene="greA"
                     /locus_tag="VFX46_05095"
     CDS             complement(525..1001)
                     /gene="greA"
                     /locus_tag="VFX46_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX5599987.1"
                     /translation="MTERVPMTAEGYRQLEAELHRLKSIERPRIIQAISEARSHGDLS
                     ENAEYHAAKEAQGLNEARVAELEDKLSRAEVIDPKTLSGDTVMFGATVTLVDEDTGEE
                     VKYQIVGDFEANVRQGRISISSPIARALIGRSKGDSVEVTTPKGTRSYEIVNVEFK"
     gene            complement(1120..>2257)
                     /locus_tag="VFX46_05100"
     CDS             complement(1120..>2257)
                     /locus_tag="VFX46_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX5599988.1"
                     /translation="ETIGYPVMIRPSYVLGGRAMEIVRDAGELDRYVARLSATLDRPS
                     ELVVSERRPLLIDRYLSDAIEVDVDCIADGKDTFIAGIMEHIEEAGIHSGDSACALPP
                     HSLDPEIIAELERQTRELALALNVVGLMNVQFAIKDGKVYILEVNPRASRTVPFVAKV
                     IGIPIARIAAQVMAGRPLASFNLKQRKLDHIGVKEAVFPFARFVGVDPILGPEMRSTG
                     EVMGLDRDFAMAFGKSQIASGQALPTSGTVFVSVKDSDKDRIVAPVRDLVEMGFRIVA
                     TRGTKRHLEQHGIPCDLVNKVLEGRPHVVDAMKNGEISIVFNTTEGARALADSKDIRR
                     TALLNHIPYYTTLAGAIAVTKAIKALQSDTLKVAPLQSYAGGPA"
BASE COUNT          419 a          740 c          677 g          421 t
ORIGIN      
        1 gatgcggaac gcttcgctca agttcgtctg caaggaggta tggcttcgga tcgagcagcc
       61 ggacacgatc tttacgagcc gcttcaaacg cggagaggtc atgccggcca tcgccgccca
      121 cgcggagggc aactacttcg ctgacgccga cacgctcgac cgcctcgaga gcgaaggccg
      181 tgtcgtgttt cgctatgccg acccgcaagg aaatgtgaca ccggaggcca accccaacgg
      241 gtcccagcgc agcatcgccg gtatttgcga cgagcgccgc cgcgtgctcg gcctgatgcc
      301 gcatccggaa aatgcgtcag acccgctgct cgggccgacg ggcggccgga ggttgttcga
      361 gagcttggtc gacaatctgg tcggagctgc gtaggcgggc tatccccgca gtggtcgccg
      421 gggccttggc agatatgctc atcccaagcg aaagggcccg attgggcccc ttactttaat
      481 tctcccacac cagttggccg aggcaatcgc ctcgacgagc gagcttactt gaactcgacg
      541 tttacgattt cgtaggagcg ggtccccttg ggcgtcgtca cctcgaccga atcgcccttc
      601 gatctgccga taagcgcacg agcgatcggc gagctgatcg agatgcggcc ctggcgcacg
      661 ttcgcctcga agtcgccgac aatctgatac ttcacctctt ccccggtatc ctcgtcgacg
      721 agcgtgacgg tggcgccaaa catgaccgtg tcgccactca gcgtcttggg atcgatgacc
      781 tcggcgcggg agagcttgtc ctcaagctcc gctactctcg cctcgttcag tccctgcgct
      841 tccttggctg cgtggtactc ggcgttctca ctcaggtcgc cgtgcgaccg cgcttcgctt
      901 atcgcctgga tgatgcgcgg gcgctcgata gacttcagcc gatgcagctc ggcctcaagc
      961 tgccgatagc cttctgcagt catgggcacg cgttcggtca tagtcaaagt ctcctcgccg
     1021 acgcagagtg cgccggagca tcgcctgtcg tcatttcact caccagggga agttaggtca
     1081 gccccgatgc gcaacaagct aatgcacgta ggcccgcaat caagcgggcc ctccagcata
     1141 gctctgcagg ggcgcgacct tcaatgtatc ggactgcaat gccttgatgg ctttcgtcac
     1201 tgcgatcgca cctgcgagag ttgtataata tggaatgtga ttcagcaagg ccgtacgccg
     1261 aatgtcctta gaatccgcaa gggcacgggc cccctcggtc gtattgaaca cgatcgagat
     1321 ctccccgttc ttcatggcat cgacgacgtg tggccgcccc tccagcacct tgttgacgag
     1381 atcgcagggg atcccatgct gctccaggtg gcgcttggtc ccccgcgtcg ccacgatgcg
     1441 gaagcccatt tcgaccagat cccgcacggg cgccacgatc cggtccttat ccgagtcctt
     1501 caccgagacg aacacggtcc ccgaggtcgg caaggcctgg ccagaggcga tctggctttt
     1561 cccgaaagcc atcgcgaagt cgcgatcaag acccatgacc tcgccggtgg atctcatctc
     1621 gggccccagg atcggatcga ccccgacaaa gcgggcgaac gggaatacag cctccttgac
     1681 gccgatgtgg tcaagcttcc gctgcttcag gttgaaggac gcaagcggcc ggcccgccat
     1741 cacctgagcg gcgatgcggg caatcggaat cccgatcacc ttggcgacga aaggcaccgt
     1801 gcggctcgcg cgcggattga cctccaggat gtagaccttg ccatccttga tggcgaactg
     1861 cacgttcatg aggccgacga cattgagcgc caacgcaagc tctcgcgttt gccgctcgag
     1921 ctccgcgatg atctctgggt cgagcgaatg cggcggcaga gcacaggccg agtcgcctga
     1981 gtggatgcca gcctcctcga tgtgctccat gatgccggca atgaacgtgt ccttgccgtc
     2041 ggcgatgcag tcgacgtcga cctcgatggc atcagacagg tagcggtcga tcagaagcgg
     2101 ccgccgctcc gagacgacca gctcagacgg ccggtcgagc gtcgccgaca gccgcgcaac
     2161 ataccggtcg agctcccccg cgtcgcgcac gatctccata gcccggccgc cgagcacata
     2221 ggacggccgg atcatgacgg ggtatccgat cgtctcc
//
LOCUS       DASUMG010000311         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_522869, whole genome shotgun sequence.
ACCESSION   DASUMG010000311 DASUMG010000000
VERSION     DASUMG010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522869"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFX46_05105"
     CDS             complement(<1..542)
                     /locus_tag="VFX46_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX5599989.1"
                     /translation="MSHFQATMRDDGSQERTEAKEAELTFEARALQLAEHIDIKGLER
                     EGQLSTNPLALRVGEKGMAVLFRFGAAVFIRMTPREQEEFIAEISSRLIEPTTVRESE
                     SVSITIRPDAEDSVTLSGNVVLKNDAPERLLLVAEALAVSVALGYDEIRIAEAFDRIE
                     PVANSLMRGKLLRGSRAALVR"
     gene            complement(539..2278)
                     /locus_tag="VFX46_05110"
     CDS             complement(539..2278)
                     /locus_tag="VFX46_05110"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5599990.1"
                     /translation="MPRTFKIALAQLNPVVGDIPGNVEKAKRAHAEAAQLGADVIVFP
                     ELFITGYPPEDLVLKPAFAAAARRGLEDLARDVANGGPALIVGTVWAEEGRAYNSVAL
                     LDEGAIQAVRYKVDLPNYGVFDEKRVFHSGPLPGPINVRGVRLGVPICEDIWQEEVVE
                     CLQESGAEIIVSPNGSPYDRLKLDLRFNVAVARITESGLPLAYLNQVGGQDELVFDGA
                     SFVLNSDANLAVQLPAWEEAVVVTEWEQRDGSWWCKPGLKAVIEEDAAAAYQACMLGL
                     RDYVEKNGFPGVVLGLSGGIDSALVAALCVDALGPERVSCYMLPYRYTSNESLSSAAA
                     CAAALGVHYEILPIEAPVAGFMSALEKPFAGREPDTTEENIQSRVRGTMLMAISNATG
                     RMLVTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYRLARWRNAHVPKGGKGPTG
                     VVIPECILTRVPSAELRPNQTDQDTLPPYEVLDDILECLVEHEMPLPDIVARGHDPAT
                     VKRVERMLYLAEYKRRQSAPGVKITTRSFGRDRRYPITNKFRESLEAPASTQSTIAPP
                     RLVRPRADGGEVS"
     gene            complement(2377..>3168)
                     /locus_tag="VFX46_05115"
     CDS             complement(2377..>3168)
                     /locus_tag="VFX46_05115"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012109973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX5599991.1"
                     /translation="QALADRISETLVFMRACGITSENTPQLRTTNVYTSHEALLLGFE
                     QALTRLDSTSGEWYATSGHMLWIGDRTRQPDHAHVEFCRGIRNPIGVKCGPTLEPDEL
                     LRLIDILDPKNEPGRLTLICRFGADKLESHLPKLIRAVRREGRTVVWSCDPMHGNTIT
                     AASGYKTRPFDRILKEVELFFDVHRAEGTHAGGIHVEMTGQNVTECTGGATAITEADL
                     SDRYHTHCDPRLNADQALELAFLIAERLRQERMNGVQQEAAAALG"
BASE COUNT          548 a         1038 c          988 g          594 t
ORIGIN      
        1 cggaccagcg ccgcacgact gcctcggagc agcttgccgc gcatgaggct gttggcgaca
       61 ggctcgatgc ggtcgaaggc ttccgcgatc cggatctcgt cgtatcccag ggccacactg
      121 acagccaacg cctccgcgac gagcagcagg cgctcgggcg catcgttctt cagaacgaca
      181 ttcccactaa gcgtgacaga atcctcagcg tctggccgga tggtgatgct gacgctctcg
      241 ctttcgcgca ccgtcgtggg ctcgattagg cggctgctga tctcggcgat gaactcctcc
      301 tgctcacgag gggtcatgcg gatgaagacc gccgccccga agcgaaacag caccgccatg
      361 cccttctcgc caacccgcaa ggcgagcgga ttggtggaaa gctgcccctc gcgttccagt
      421 cccttaatgt cgatatgctc cgcaagctgg agcgctcgcg cctcgaacgt cagctcggct
      481 tcctttgcct ccgtgcgctc ctgggagcca tcgtcgcgca tagttgcttg aaagtggctc
      541 aagacacctc gccaccgtca gcgcgcggcc tgacgaggcg tggaggcgca attgttgatt
      601 gggtggaggc cggcgcctca aggctttcgc ggaacttgtt cgtgatcgga tatcgccggt
      661 cgcgcccaaa gctgcgcgtc gtgatcttca cgccgggagc cgactgccgg cgcttgtact
      721 cggccagata gagcatccgt tctacacgct tcacggttgc cgggtcatgg ccccgagcaa
      781 cgatgtcggg cagtggcatc tcatgctcga caagacattc gaggatgtcg tcgagcacct
      841 cgtacggtgg caacgtgtct tggtcggtct ggtttggccg tagctccgcg gagggcacgc
      901 gcgtcaaaat gcactctggg atgacgacgc cagttggccc tttgccaccc ttcggcacgt
      961 gcgcattgcg ccagcgcgcc aggcggtaaa cctgcatctt gtaaaggtcc ttgatcgggt
     1021 tgaagccccc attcatgtcg ccatagagcg tggcgtagcc gacagacatc tcggacttgt
     1081 tgccggtcgt cacgagcatc cttccggttg cattcgagat ggccatcagc atcgtgccgc
     1141 gcacgcggct ttggatgttc tcctcggtgg tgtccggctc tcgaccggcg aagggcttct
     1201 ccaatgccga catgaagccc gcgacgggag cctctattgg caagatctcg tagtgcacac
     1261 ccagagctgc ggcgcaggcc gccgcgctgg agaggctttc gttcgaggtg taccggtacg
     1321 gcagcatgta gcagctcacg cgctccggcc cgagcgcatc gacgcacagg gcggccacga
     1381 gcgcggagtc gataccgccg gacagcccaa gcaccacgcc agggaacccg ttcttctcca
     1441 cgtaatcgcg caggcccagc atgcatgcct gataggccgc ggcggcatct tcctcgataa
     1501 cggccttgag gccaggcttg caccaccagc ttccgtcccg ctgctcccac tcggtgacga
     1561 cgaccgcttc ctcccaagct gggagctgca cggcgaggtt ggcatccgag ttgagcacga
     1621 aggaagcgcc atcgaagacg agctcgtcct ggcctccgac ctggttcagg tatgcgagcg
     1681 gcagcccgct ctcggtgatc cgcgcaacag cgacgttgaa gcgaaggtcc aatttcaacc
     1741 ggtcataggg ggagccgtta gggctgacga tgatctctgc gcccgactct tgcaggcact
     1801 cgaccacctc ctcctgccag atgtcctcgc agatcggcac gccgaggcgc accccgcgca
     1861 cgttgatcgg cccaggcagc ggcccggaat gaaatacccg cttctcgtcg aagacaccgt
     1921 aattgggcag gtcaaccttg tagcgcactg cctggatggc gccttcgtcc agcagcgcca
     1981 cggagttgta ggcacgaccc tcctcggccc atacggtgcc gacaatcaat gccgggccgc
     2041 cgttcgcaac gtcccgggcc agatcttcca gccccctgcg cgccgccgcg gcgaacgccg
     2101 gtttgagcac gaggtcctcg ggcgggtagc cggtgatgaa cagctcaggg aagacgatga
     2161 catcggcccc gagctgagcc gcttccgcgt gcgcgcgctt ggccttctcg acattaccgg
     2221 ggatgtcgcc gaccaccgga tttagctgag ccagcgcaat cttgaatgtt ctgggcatgc
     2281 aacagtttta agcgggaatg ggcgctgagc gaatagcgaa tgatgggcgc gccaagttcc
     2341 gctgatcacg gctcggtgcg cgcccattcg cttgcctcaa cccaaggcag ctgccgcctc
     2401 ctgttgcacg ccgttcatcc gttcctgcct cagccgctca gcgatcagga aagcgagctc
     2461 gagtgcctga tcggcattga gccgcgggtc gcaatgcgtg tggtagcggt cggagagatc
     2521 ggcttccgtg atcgccgtcg cgccacccgt gcactcggtc acgttctggc ccgtcatctc
     2581 cacgtgaatc ccgccggcgt gcgtgccctc ggcccgatgg acatcgaaga acagctcgac
     2641 ttccttcagg atgcgatcga acggccgggt cttgtagccc gaagcggccg tgatcgtgtt
     2701 gccgtgcatc gggtcgcagg accacacgac ggtccgccct tccctccgaa cagctcggat
     2761 gagcttcggc aggtgtgact ccagcttgtc tgcgccgaac ctgcagatga gcgtcagccg
     2821 gccaggctcg ttcttggggt cgaggatgtc gatcaggcgc aacagctcgt ccggctcgag
     2881 ggtaggcccg cacttgacgc cgatcgggtt gcggatgcca cggcagaact ccacgtgggc
     2941 atgatccggc tgtcgggtgc gatcgccgat ccacagcatg tggcccgagg ttgcgtacca
     3001 ttcgccgctc gtggaatcga gccgggtcaa cgcctgctcg aagccgagca gcaacgcctc
     3061 atggctcgtg tagacgttgg tcgtcctgag ctgcggggtg ttctccgacg taatcccgca
     3121 agcccgcatg aagaccagcg tctctgaaat gcggtctgcg agcgcctg
//
LOCUS       DASUMG010000312         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_201905, whole genome shotgun sequence.
ACCESSION   DASUMG010000312 DASUMG010000000
VERSION     DASUMG010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201905"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VFX46_05120"
     CDS             complement(<1..708)
                     /locus_tag="VFX46_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX5599992.1"
                     /translation="MRAIVAVAAAVAACLLALFGTVGSALAQQEAPAQPAEESTEAAS
                     PEWTGTVTPAGSAEAEFTEEQLATIAKVNAYFNELDNLQGRFLQTDPDKKQMRGKFYV
                     KKPGRFRFDYGSPSLKVIISDGRWLAIQDHDLNTEDVYELDNTPFRLLLRKDVDLLRD
                     ARIFDVQEVEDLIIITLQDKDPDVSGRITLFLTKDPELMLKEWVTVDAQGLETRVQLS
                     QVDATAEIDPNLFKRENL"
     gene            complement(847..>2592)
                     /locus_tag="VFX46_05125"
     CDS             complement(847..>2592)
                     /locus_tag="VFX46_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX5599993.1"
                     /translation="LMPRRSVGQADLGRAPSEQEANSLTDGPALDPEFEDEESRRIAQ
                     RFAPANAQAAPRPARPASSGLLRFPLHRGDSSAFRKPGVNLLKKPAGGKPGPEFTQTV
                     LRGTARLLEDVLADFGIKGEIKDIRPGPVVTLFELEPARGIKSSRIISLAEDIARSMS
                     ATAVRVAVVPGRNVIGIELPNTRREIVLLRELFETEAFRSTDARLPLALGKAINGDPV
                     VVDLARMPHLLIAGTTGSGKSVGINSMVLSLLYKLTPEDCRLLMIDPKMLELSVYNGI
                     PHLLTPVVTDPHKAVAALNWVVREMEERYKRMAALGVRNIDVFNNRVRNAKIRGDRMR
                     RTVQTGFDRRTGEAVFETEELDLEPMPYIVVVVDEFADLMIVAGKEIEAAVQRLAQMA
                     RAAGIHLIMATQRPSVDIITGTIKANFPTRISFKVASKVDSRTILNEQGAEQLLGQGD
                     MLFSPGSGQIIRVHGPFVSDEEVESIAQFLREQGEPEYVEGITDGPQEPEDFGAAGNG
                     KSSEGDLYEKAVAIVLRDRKASTSYLQRRLSIGYNRAADLMERMEREGIVSPANAVGK
                     REILVSGLSATSTAP"
BASE COUNT          479 a          833 c          760 g          520 t
ORIGIN      
        1 gagattctcc cgtttgaaca aattggggtc gatctccgcg gtcgcgtcca cctgcgagag
       61 ctgaacacgc gtttccaacc cttgcgcatc gacggtcacc cactccttga gcatcagctc
      121 tggatccttg gtcaggaaga gtgtgatccg gccggacacg tccgggtctt tgtcctgcag
      181 tgtgatgatg atcagatcct caacttcctg cacatcgaag atgcgcgcat cacgcagcaa
      241 atcgacgtct ttgcgcagaa ggagtcggaa gggcgtattg tcgagctcgt acacgtcctc
      301 agtgttgagg tcgtgatcct gaatggcgag ccacctccca tcggagatga tgaccttcag
      361 gctgggggag ccgtaatcga agcgaaaccg gcccggcttc ttgacgtaga acttaccccg
      421 catctgcttc ttgtcggggt cggtctgcag gaagcggccc tgcaggttat ctagctcgtt
      481 gaaataagcg ttgaccttcg caattgtggc cagctgctct tccgtgaact ccgcttctgc
      541 ggagcccgct ggggttaccg ttccggtcca ctccggacta gcggcctcag tgctctcctc
      601 agccggctgg gctggagcct cttgttgcgc cagagccgag ccaaccgttc caaacaatgc
      661 gagcaggcaa gcggcaaccg ccgccgctac cgccacaatc gcgcgcattc cgcccccttt
      721 tctggtcaag gctcgtggag atcgttgtcg caacaatgag accagatgtg ggcaatctat
      781 ctgcccgaat cagttgcaca agctcctccc aactaccgga ctgtggacgg agcaaatttg
      841 ttcatctcag ggcgcggtcg aggttgccga aagcccgctc accaggatct cgcgcttgcc
      901 aacggcattc gccggactga cgataccctc ccgctccata cgttccatga gatcggcagc
      961 gcggttgtag ccgattgaaa gccggcgctg caggtagctc gtcgacgcct tgcgatcgcg
     1021 cagaacgatg gcgacagcct tctcgtagag atcgccctcg ctgctcttcc cgttgcctgc
     1081 cgccccgaag tcctcgggtt cctgaggacc atcggtgatg ccttcgacgt attccggctc
     1141 gccctgctct ctcaagaact gcgcgatgct ctcgacctcc tcgtcggaaa cgaaggggcc
     1201 gtgaacacgg atgatctggc ccgaccccgg cgagaacagc atatcgcctt gcccgagcag
     1261 ctgctccgcg ccctgctcgt tcaagatcgt gcggctatcg actttcgagg ccaccttaaa
     1321 gctgatgcgc gtcgggaaat tggccttgat ggttccggtg atgatgtcga ctgatggccg
     1381 ctgcgtcgcc atgatgaggt gaatccctgc cgcgcgcgcc atctgcgcca gccgctgcac
     1441 cgcagcctcg atctccttcc cggcgacgat catcaaatcg gcaaactcgt cgaccacgac
     1501 gacgatgtag ggcatcggct cgagatcgag ctcctcggtt tcaaaaactg cctcgccggt
     1561 gcgccgatcg aatccggtct gcacggtgcg ccgcatccgg tcgccgcgga tcttcgcgtt
     1621 ccgcactcga ttgttgaata cgtcgatatt ccgcacaccg agcgccgcca tgcgcttgta
     1681 gcgctcctcc atttcgcgca cgacccagtt gagcgcagcg actgccttgt gcggatcggt
     1741 gacgaccggc gtcagcagat gcgggatgcc gttgtagacc gacagctcca gcatcttcgg
     1801 gtcgatcatc agcaaccggc aatcctcggg cgtcagcttg tagagcaacg acagcaccat
     1861 cgagttgatg ccgaccgatt tgccggagcc cgtcgtgcca gcaatcagga ggtgcggcat
     1921 acgggccaag tcaacgacga ctgggtcacc gttgatggcc ttgccgaggg cgagcgggag
     1981 ccgcgcgtcg gtggagcgga aggcttccgt ctcgaacagc tcccgaagca gcacgatttc
     2041 gcgccgcgtg ttaggcagct caattccgat cacattacgg cccgggacca cggcgacccg
     2101 cactgccgtg gcactcatcg accgggcaat gtcctcggcc aagctgatga tccgcgagga
     2161 tttgatgccg cgcgctggtt ccagctcgaa aagcgtaact accgggccag gcctgatgtc
     2221 tttgatctcg cccttgatgc cgaagtccgc caggacatct tcgagcaacc gcgccgtgcc
     2281 tcgcagaacg gtctgggtaa actcaggacc cggcttacct cccgccggct tcttcaacag
     2341 attgacgccc ggcttgcgaa aggcagaaga gtcgccccgg tggagcggaa accggagcag
     2401 accggagctc gcgggtcgcg ctggtcgcgg agccgcttgc gcattggctg gagcaaatcg
     2461 ctgcgcaatg cggcggcttt cctcatcttc gaattccgga tcgagcgccg gaccgtcagt
     2521 cagactgttg gcctcctgtt ccgaaggagc gcgccccaag tcggcttgtc caacggaacg
     2581 ccttggcatc ag
//
LOCUS       DASUMG010000313         3082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_763509, whole genome shotgun sequence.
ACCESSION   DASUMG010000313 DASUMG010000000
VERSION     DASUMG010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763509"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFX46_05130"
     CDS             complement(<1..480)
                     /locus_tag="VFX46_05130"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5599994.1"
                     /translation="MKHRIQFLRRRREFITLLGGAGLAWPVAARGQTWPTQLTRIICP
                     IAAGGGVDATARIVASQLSQIWGQQVVVENKTGASGNMAAEYVARSSPDGYVIYIAAF
                     PHATNRYLYSSLGYDPVADFAPVTLIGFYPLVMVVPNSSPAHSVKEFIAYAKTKKLSY
                     "
     gene            complement(477..1703)
                     /locus_tag="VFX46_05135"
     CDS             complement(477..1703)
                     /locus_tag="VFX46_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5599995.1"
                     /translation="MKTLHKRCAGLDVHKAEVVACVRLISKRKVEREVRRFPTTTQGL
                     LALAEWLESAGCTHVAMEATGVYWKPVWHILEGRFALILANAAHVRNVPGRKSDVNDA
                     TWLADLLAHGLIRSSFVPPPAIQELRDLTRTRRQLTREIVQHVQRIQAVLEEANIKLC
                     SVITDIMGASGRRMLKALIAGETDAEKLAALGHERLGCTRAELVEVLTGRVREHHRFL
                     LGQHLRTIEQLKDSVVAFDARIEAALSPFHDIVERLEEVPGLAATSTETVIAEIGTDM
                     SLFPTAGHLLSWAGFAPRLDESAGKHRSTRIRKGAPWLKPVLVQAAWGAARKKNSYFQ
                     AQFLRLKARHGAKKAAIAVAASILTTVYHMLRDGTCYQDLGPEYFTRRNPAQAAARLA
                     NRIRNLGYHVEIRAAA"
     gene            complement(1989..2258)
                     /locus_tag="VFX46_05140"
     CDS             complement(1989..2258)
                     /locus_tag="VFX46_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX5599996.1"
                     /translation="MGNSFALLWPAIVLCAPTFVWAEAAPPAGSKPLTEILRPLEAGS
                     DFAYFEEIELEGGKYKIEYFKKDGSKQKIEVDPLTGTPSEPAPAR"
     gene            complement(2317..>3082)
                     /locus_tag="VFX46_05145"
     CDS             complement(2317..>3082)
                     /locus_tag="VFX46_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX5599997.1"
                     /translation="ILAISWTSHDYVNHLFGPESRQSQDQTARLDRIFAELFDYLDQR
                     IGLQNVLITLSADHGFMNIPEYSASRNLDAGRIDPDKMIEATNTALSAKFGDGKYITA
                     WWNPNLYVDYKLIASRNLDKVEVENAAQDFLRDYPGVEAVYTRTQMERGMMPNTKLAK
                     QVILAWHQKISGDIVIMNKPNWYLFAKPNTYASTHGSPWSYDTNVPLAMYGPNWIKPG
                     KYGDSEVVDLGRTIAFLLNVRPPNGCEGRVLSEALN"
BASE COUNT          580 a          954 c          905 g          643 t
ORIGIN      
        1 atagctgagc ttcttcgtct tcgcgtaggc gatgaactcc ttcaccgagt gcgccggaga
       61 ggagttcggc acgaccatca cgagggggta aaaaccaatc agcgtgacgg gggcgaagtc
      121 agcgactgga tcgtatccaa gcgaggaata aagatagcga ttggtggcgt gcggaaacgc
      181 agcaatataa atgacgtaac catctgggct ggaacgggca acgtactcgg ccgccatgtt
      241 accgctggcc cctgtcttgt tttcgaccac gacctgctgg ccccaaatct gggagagctg
      301 cgaggcgacg atacgggcag tggcatcgac ccctccaccg gccgcgatcg ggcagatgat
      361 gcgcgtaagc tgcgttggcc acgtctgccc gcgcgctgca actggccaag cgagacccgc
      421 accgccgagc agtgtgatga actcccgccg tcttcttaga aactggattc ggtgcttcat
      481 gcggcagctc tgatttcaac atgataaccg agatttcgga tgcggttggc gagtctggcc
      541 gcggcttggg ccggattacg gcgagtgaag tattcggggc cgaggtcctg gtagcaagta
      601 ccatcacgca gcatgtgata gacggttgtc aggatcgacg cggcaaccgc gatcgctgcc
      661 ttcttggcgc cgtggcgcgc tttgagacgg aggaattggg cctggaaata gctgttcttc
      721 ttgcgcgctg cgccccacgc agcttgcacc agtaccggct tgagccaggg tgctcccttc
      781 ctaatccgcg ttgagcggtg cttgccggcg ctctcgtcaa ggcgcggagc gaatcccgcc
      841 caggagagaa gatgccccgc ggttgggaac aggctcatgt cggttccgat ctcggcgatc
      901 acggtctcgg tggaggtcgc agcaagcccc ggcacttcct ccagccgctc gacgatgtca
      961 tgaaagggcg atagcgcggc ctcaatgcgg gcgtcgaagg ctacaacgct gtccttcagc
     1021 tgctcaatcg tccgcagatg ctgaccgagc aggaaccgat gatgctcgcg gacacggccg
     1081 gtgagaactt ccaccaactc agcccgcgtg cagccgaggc gctcatgacc aagcgcggcg
     1141 agcttttcgg catccgtctc gccggcgatc agggccttca acatgcgccg cccgctggcg
     1201 cccatgatgt ccgtgatcac cgaacacaac ttgatgttcg cctcctccag aaccgcttgg
     1261 atacgctgca cgtgctgcac gatctcgcgc gtcaactgcc tgcgcgtccg ggtcagatcg
     1321 cgcagctcct gaatcgccgg tggcggcacg aagcttgacc ggatcaggcc atgcgccaac
     1381 aggtccgcaa gccaagtcgc gtcgttcacg tcactcttgc ggccaggtac gttcctcaca
     1441 tgcgcggcat tcgccagaat gagtgcaaac cgcccttcca gaatgtgcca gaccggcttc
     1501 cagtacacgc ccgtcgcttc catcgccacg tgcgtgcatc cggcactctc cagccactcg
     1561 gcaagcgcca ataatccctg tgtcgtcgtc gggaaacggc gcacctctcg ctccaccttc
     1621 cgcttgctga taaggcgcac gcaggccacc acctctgcct tgtgcacatc gagacccgcg
     1681 caccttttat gcaacgtctt catcgccacc tccctaatta aggcgcggcc ggcatgaagc
     1741 ccttgctctc tgaactcttc ttaccgtgtt ccgggatgca ttcacccatt cgggcaaatc
     1801 gctatccgtt ggccacaatt accggtgctc aaagggcctc cggtcaaact cattcacggg
     1861 ctcgctcgca ccaagacgac gccgacctcc gcgccgaccg cagcacagct taccttgttt
     1921 catgcatccg ggtgcgcagc gcccatggca actcattcga atcctgatcg ccgtcgtcca
     1981 ttccgtagtt agcgagccgg cgcaggttca ctgggcgtac cggtcagcgg atcaacctca
     2041 attttctgtt ttgacccatc ctttttgaaa tattcaatct tgtatttgcc tccttccaac
     2101 tcgatttctt cgaaataggc gaaatcggag cctgcttcga ggggccgcag aatttcggtc
     2161 agcggctttg agcccgccgg cggggcggcc tcggcccaga cgaacgtcgg cgcgcagagc
     2221 acgatggcgg gccacagcaa tgcaaatgag tttcccattt cgacctcctg agcgatcggc
     2281 cggccgccgg gggcggcacc tatctcggca ttgctcctag ttcagggcct cggacaacac
     2341 gcggccctcg cagccgttcg gcggacgtac attcagcagg aaagcgatcg tccgcccgag
     2401 gtccacgact tcggagtcgc cgtatttgcc aggcttgatc caattcggac cgtacatggc
     2461 cagcggcacg ttcgtgtcgt acgaccaagg cgatccgtgc gtcgaggcgt aggtgtttgg
     2521 ctttgcgaac agataccagt tcggcttgtt cattatgacg atatcgccgc tgatcttctg
     2581 gtgccacgcc aggatcacct gcttcgcaag cttggtgttc ggcatcatgc ctcgttccat
     2641 ctgcgtgcgg gtgtacacgg cctccacgcc tggatagtcg cggaggaagt cctgcgctgc
     2701 gttctcgacc tcgactttat cgaggttacg ggacgcgatc agtttgtaat cgacatagag
     2761 gttcgggttc caccaggcgg tgatgtattt gccgtcaccg aacttggcgg aaagcgcggt
     2821 gttggttgcc tcgatcatct tgtcgggatc gatgcgccca gcatcgagat tacggctggc
     2881 gctgtactct gggatattca tgaatccatg gtcggcggac agcgtgatca gaacgttctg
     2941 caggccgatg cgctggtcga gatagtcgaa caattccgcg aagatgcggt ctaggcgggc
     3001 ggtctggtcc tgtgactggc ggctctctgg gccgaacaag tggttcacgt agtcgtggct
     3061 cgtccagctt atagcgagaa ta
//
LOCUS       DASUMG010000314         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_843952, whole genome shotgun sequence.
ACCESSION   DASUMG010000314 DASUMG010000000
VERSION     DASUMG010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843952"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFX46_05150"
     CDS             complement(<1..385)
                     /locus_tag="VFX46_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012484724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5599998.1"
                     /translation="MQLKVNRFVGMGAIGALLVLVGASLALTSTSAQEDDANRQLIEE
                     GREIFRFDTFGDEVFWGGTLQLHQAIAGEQLGGVGPGVSPNTALSVGLKVDAEALPAE
                     LVEQIQAGEVDLDDPATTLALLELNA"
     gene            complement(564..1235)
                     /gene="folE"
                     /locus_tag="VFX46_05155"
     CDS             complement(564..1235)
                     /gene="folE"
                     /locus_tag="VFX46_05155"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5599999.1"
                     /translation="MMDWDPIGTAALDVSHGNGHRPSRASDEVTIPYPDREEKIKLLL
                     RSLLVLLGEDPERSGLRGTPQRMARMYGELLAGYETDIETLVNGALFDVDYSEMVVVK
                     DIEYYSLCEHHLLPFFGKAHVAYIPRDRVIGLSKIPRIVEMYARRLQVQERMTQQIAT
                     QLDDVLHPQGVAVVMEGTHLCAAMRGVEQRSARMRTSAMLGCFQKNAALRAEFLQQIG
                     PEPAH"
     gene            complement(1244..>2308)
                     /locus_tag="VFX46_05160"
     CDS             complement(1244..>2308)
                     /locus_tag="VFX46_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5600000.1"
                     /translation="EKHYPYDRPPLSKQLWTGKKTQEDIWRDVPEGVSFYLGRTVVAL
                     DLDRKEARDQAGDVYTFDKLLLATGGKPRRLPFGGDNIIYFRSLDSYRRLRALAEQHD
                     RFVVIGGGFIGSEIAAALAMQGKHVTMLFPEGGIGARQFPADLAAHLNEYYREQGVDV
                     LSRESVVDVEGAGNELTVVTESGRRIEANAVVAGIGITPNVALAEAAGLNVTDGIVVD
                     SSLRTSHPDVYAAGDVANFPDPVLGTRRRVEHEDAANSMGAVAGRAMAGAEAVYDHTP
                     MYYSDMFDLGYEAAGRLDSRLETVVDWQEPFRKGVIYYLDGQRVCGVLLWNVWDKVDE
                     ARQLLAEPDRFSIRTLQQGR"
BASE COUNT          414 a          780 c          681 g          433 t
ORIGIN      
        1 cagcgtttag ctcaagcagc gccagggtcg tcgccggatc gtcaaggtcg acttcgcccg
       61 cctggatctg ctcgaccaac tcggcaggca atgcttcggc atcgaccttc aacccaaccg
      121 acagcgctgt gttcgggctc acccctggac cgacgccccc aagctgctcc cctgcgatgg
      181 cctgatgaag ctggagcgtg ccgccccaga agacctcatc gccaaaggta tcgaagcgga
      241 agatctcgcg gccctcttcg atgagttgcc ggttggcgtc atcctcttgc gccgaggtgc
      301 ttgtcagagc cagactggcg cccaccagca caagcagcgc accgattgca cccatcccta
      361 cgaagcgatt gactttaagc tgcatctcca tcacctcatg tatgaagaac cgattgcttg
      421 ctcataagca catcagtcgg tcgagaggcg cctcgttcac ctgtctggac gagcgatcgt
      481 ccagacaggc gggtgaggag cgcaaagagc catcgcttgc cggtatacgc cgacggtgca
      541 gcgaaaaaac aaccgccccg gctctagtgg gccggctcgg gcccaatctg ctgcaaaaac
      601 tccgcacgga gcgccgcatt cttctggaag cagccgagca tggcgctggt gcgcatccgc
      661 gcgctgcgct gttcgacgcc gcgcatggcg gcgcagaggt gcgtcccttc catcacgacg
      721 gcgactccct gcggatgcag cacgtcgtcc aactgcgtcg cgatctgttg cgtcatgcgt
      781 tcctgaacct gcaggcggcg cgcgtacatc tccacgatgc gcgggatctt cgacagcccg
      841 atgacccgat ctcgcgggat ataggcgacg tgtgccttac cgaagaacgg caagaggtga
      901 tgctcgcaca ggctgtaata ctcgatgtcc ttcacgacga ccatctcgct gtagtccacg
      961 tcgaacagcg ccccgttgac cagcgtctcg atgtccgtct cgtaccctgc cagcaactct
     1021 ccgtacatcc gcgccattcg ttgcggcgtg ccgcgcaagc cactccgctc cggatcctcg
     1081 cctagcagca caagcagaga gcgcaggagc agcttgatct tctcttcgcg atccgggtag
     1141 gggatggtca cttcgtcgct agcccggctg ggccggtgac cgttgccgtg cgatacatcg
     1201 agtgctgccg taccgattgg atcccaatcc atcatgcctt ctcctatcgt ccctgttgga
     1261 gcgttcggat cgagaaacga tctggctcgg cgagaagctg gcgggcctcg tctaccttgt
     1321 cccagacgtt ccagagcaag acgccgcaaa cgcgttgccc gtcgaggtag tagatgacgc
     1381 ctttgcggaa aggctcctgc cagtcgacga ccgtttccag acgcgaatca agccgccccg
     1441 ccgcctcata gccgaggtca aacatatccg agtagtacat cggcgtgtgg tcgtacactg
     1501 cttcggcacc ggccatcgcc cggcccgcga ccgcgcccat gctgttggca gcgtcctcat
     1561 gctcgacccg gcggcgcgtg ccaagcaccg gatcggggaa gttggcgaca tcgcccgccg
     1621 cgtacacgtc cggatggctg gtgcgcaggc tgctgtccac aacgatcccg tcggtcacgt
     1681 tcagccccgc cgcctcggcc agcgccacgt tcggcgtgat gccgatgccc gccacgactg
     1741 cgtttgcctc aatccggcgg ccgctctcgg tgacgacggt cagctcgttt cccgcgcctt
     1801 ccacgtccac gaccgattcg cgagacagga cgtcgacgcc ttgctcgcgg taatactcat
     1861 tcaggtgagc cgccaggtct gcggggaact ggcgggcgcc gatcccacct tcgggaaaca
     1921 gcatggtgac gtgcttgccc tgcatcgcca gcgccgctgc gatctcggag ccgatgaagc
     1981 cgccgccgat caccacaaag cgatcgtgct gctcggccag ggcgcgcaga cggcggtagc
     2041 tgtccagact gcgaaagtaa atgatattgt cgccgccaaa gggcaggcgt cgcggcttgc
     2101 cgccggtggc aagcagcaac ttgtcgaagg tgtacacgtc tccggcctga tctcgcgcct
     2161 ctttgcggtc gaggtccagc gccacgacgg tgcgccccag atagaaagag acgccttccg
     2221 gcacgtcgcg ccagatgtcc tcttgcgttt tcttgcccgt ccacaactgc ttgctaagcg
     2281 gtggacggtc gtacgggtag tgcttttc
//
LOCUS       DASUMG010000315         4904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_924645, whole genome shotgun sequence.
ACCESSION   DASUMG010000315 DASUMG010000000
VERSION     DASUMG010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924645"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1176
                     /locus_tag="VFX46_05165"
     CDS             190..1176
                     /locus_tag="VFX46_05165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003535421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HEX5600001.1"
                     /translation="MTFKALLAAKTDEKISTSVVEMTEQDLMPGDVTIAVDYSTVNYK
                     DALAITGRADVIRQFPLIPGIDLAGTVEASSYPGIAVGDRVVANSWGLSQTHHGGYAQ
                     KARLKGEWLVKIPAPFSTKDAMAIGTAGYTAMLSVLALEHGGLTPQRGDILVTGANGG
                     VGSIAIAILSDLGYRVVASTGRLEESDYLRSLGAADIIDRRTLSEPGAPIAGERWAGA
                     VDSVGSHTLVNVLAQTQYRGVVTACGLAQGLDLPGSVLPFILRNVTLAGIDSVNAPQE
                     ARIEAWSRLARDLNPYKLARTTQVVGLAEVPDVVGRMFEGKVRGRTVVDVNA"
     gene            complement(1504..1788)
                     /locus_tag="VFX46_05170"
     CDS             complement(1504..1788)
                     /locus_tag="VFX46_05170"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5600002.1"
                     /translation="MSSQQTPTTTHSVETPSGRISYASAGSGPVALFVHGVVLNKHLW
                     RHQLAGLSDIRRCIAVDLLAHGDTEIAPDQDVSVTANAKMLREVLDGRMA"
     gene            complement(1792..2574)
                     /locus_tag="VFX46_05175"
     CDS             complement(1792..2574)
                     /locus_tag="VFX46_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX5600003.1"
                     /translation="MNQTEKAKRFAELQVKGKPLLLYNAWDAGSAKAILAAGAKAIAT
                     SSWSVAEAQGYRDGEAIPIALAEQIVARIAATIDAPVTVDFEGGYSEDDGELAGNVSR
                     LLDLGVIGINFEDRVVKGAGLYDIDRQARRISAIRKAAEQKGVELFINARTDAFFEHG
                     DDAAQAVGEALDRAKAYAVAGASGYFVPGLVDDALIGRVCESVSLPVNVMVMEGVPSN
                     ARLAELGVARISYGPIPYIRAMKALEREARTTLNEAAARSEG"
     gene            2835..3494
                     /gene="mug"
                     /locus_tag="VFX46_05180"
     CDS             2835..3494
                     /gene="mug"
                     /locus_tag="VFX46_05180"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEX5600004.1"
                     /translation="MSSYEPDILARDLDVIFCGVNPATTAAVVGHNFSSCSNRFWTVL
                     HLTGFTDVRLQPHEERRLLEYRCGLTAVVARPTRRAIDVPPDEFRKARPGFEAKIRRY
                     APRSVAFLGKRTFSAMMDQPDVAWGRYPAGFAGTTTWILPNPSGLNKAFSLEALVRTI
                     ASCGRPSGPGRQRETSGHGERPGRIDSARSRRGNLDLSWRVPSITGAIRMRLRRNRPL
                     W"
     gene            complement(3457..3708)
                     /locus_tag="VFX46_05185"
     CDS             complement(3457..3708)
                     /locus_tag="VFX46_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018858232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HEX5600005.1"
                     /translation="MLDLRPNCECCNKDLPPAATDAMICSFECTFCRGCAETTLGGRC
                     PNCGGGLVARPIRPAEKLARYPASTVPVTKADGCVGASS"
     gene            complement(3734..4408)
                     /locus_tag="VFX46_05190"
     CDS             complement(3734..4408)
                     /locus_tag="VFX46_05190"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5600006.1"
                     /translation="MFSVIFEVHPKQEKFDLYLELAKGLRPILEGIDGFIDNERFEST
                     RRPGWILSHSTWRDEKSVVRWRTVGKHHNTQQRGRDEVFQDYHLRVGEIVADTAPPAG
                     AAIVEQRLDETEIGRAKFMTLTEVPPSSGMTVAVLPDWLSLDRHRADLIDHDVFASIY
                     NAGKLALLASWRDRPSAEKFSPPAFGGLRHRIVRVVRDYGMFDRRETPQYYPDIQTGT
                     ASRGNS"
     gene            4590..>4904
                     /locus_tag="VFX46_05195"
     CDS             4590..>4904
                     /locus_tag="VFX46_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX5600007.1"
                     /translation="MSDVKTAIMDAAERRMQQGGFGGFSFREIATDVGIKSSSVHYHF
                     PTKEDLAAAVIRRWAEYTSELIDKELEKDPDPVRVWTKAFQGTAFSEAHMCPCTVLGA
                     ASQ"
BASE COUNT          883 a         1579 c         1514 g          928 t
ORIGIN      
        1 gctttcgcgc tgccgtgcga cggcggcttt gcccttagcg agggagatac ggacttgaac
       61 agtcaagatc ctcatcgcca gcgcgggctg gtgcggcggc agctcgccgg tcgaacgtcg
      121 ggggtcgaat tcctgacggc cttctcgacg ggcatccatc cggcatcacc cttcagcgag
      181 gatagtccga tgacgttcaa ggcgctgctc gcagcaaaga cagacgaaaa gatctcgacc
      241 agcgtggtcg aaatgactga gcaggatctc atgcctggag acgtaaccat cgcggtcgac
      301 tattcgactg tgaactacaa ggatgcgctc gccattaccg ggcgcgcgga cgtcattcgc
      361 cagtttccct tgatcccggg catcgatttg gctggcaccg tcgaggcatc ctcgtatccc
      421 ggcatcgccg tcggagaccg cgttgtcgcc aacagctggg ggctgagcca gacccatcac
      481 ggcggatatg cgcagaaggc gcggctgaaa ggcgagtggc tggtcaagat tccagcgccc
      541 ttttcgacaa aggacgccat ggccatcggc acggccggtt acacggcgat gctgtccgtc
      601 ctggccctcg aacacggcgg cctcacaccg cagcgcgggg acattctcgt caccggcgcc
      661 aatggcggcg tcggctccat cgcgatcgcg atcctgtcgg atctcggtta tcgcgtcgtt
      721 gcgtcgaccg gacgtctcga ggaaagcgat tatctgcgca gcctcggcgc ggcggacatc
      781 atcgaccgcc ggaccctttc ggagccggga gcaccgatcg cgggcgaaag atgggccggc
      841 gcagtggact ccgtcggcag tcatacgctc gtgaacgtgt tggcgcagac tcaatatcgc
      901 ggcgtggtca cggcctgcgg cctggctcaa ggtctggatc ttcccggatc ggtgctgccc
      961 ttcatcctgc gcaacgtcac ccttgccggt atcgactcgg tgaatgcccc gcaggaggcc
     1021 cgcatcgagg cctggtcgcg tctggcacgc gatctgaacc cgtacaagct cgcccggacg
     1081 acgcaggtgg tcggccttgc agaggttccc gatgtcgtgg gccggatgtt cgaagggaaa
     1141 gtccggggtc gcacagttgt cgacgtcaac gcgtaactgc cgtcgcgaat acaaaacggg
     1201 agaggaacca tgaacagagt cgcagagcac gccgccacaa ctccgaccag gtattgtgaa
     1261 gttggggacg ttcgttacgc ctacagagag cttcgcccag acaatgcagt cgatagaacg
     1321 cctgtcctgt tcctccatcg gttccggggc acactagacg actgggatcc gctcttcgtc
     1381 gatgagttgg ccaaaacacg gcaaatcgtc ctcttcagcg atcaggaagt cggttcgtct
     1441 acgggaacgg cagcaacgag cgtggacgaa aaggcacgga atgctgccgc tttcgcaagg
     1501 gctctaggcc attctgccat cgagcacctc tctcagcatc ttcgcgttgg cggtcacgga
     1561 aacatcttga tccggcgcga tctcggtgtc gccatgcgcc aggagatcca ccgcgatgca
     1621 gcggcggatg tcagacagcc cggccaactg gtgccgccac agatgcttat tcaagaccac
     1681 gccatgcaca aagagcgcga ccggaccgga gccagcggat gcgtagctga tgcggcctga
     1741 aggtgtctcg acgctgtgag tcgtggttgg ggtttgctgg ctgctcatgg ctcaaccctc
     1801 cgaacgcgcg gcggcttcgt tcaacgtcgt cctcgcttct cgctcgagtg ccttcatcgc
     1861 gcggatatat ggaatcggac cgtaactgat gcgcgcgacg ccgagttcgg cgagccgagc
     1921 attcgatgga acgccctcca tgaccattac gttcacgggc agcgagacac tttcgcagac
     1981 ccgcccgatc agcgcatcgt ccaccaagcc ggggacgaag tagccggacg cgcctgcgac
     2041 cgcgtaggct ttggccctgt cgagagcctc gccgacagcc tgcgccgcgt catcgccatg
     2101 ttcgaagaac gcatcggtcc gcgcgttgat gaagagctcg acgcccttct gctcggccgc
     2161 cttgcggata gccgagatcc ggcgtgcctg ccggtcgata tcgtagagac ccgcgccctt
     2221 cacgacccgg tcctcgaaat tgataccgat gacgccaaga tcgagcagtc gcgaaacatt
     2281 gccggccagt tcgccgtcgt cttcgctgta gccgccctcg aagtcgacgg tgaccggcgc
     2341 atcgatcgtc gccgcgatcc gcgccacgat ctgttcggcg agcgcgatcg ggatggcctc
     2401 gccgtcgcga tagccttggg cttccgcgac ggaccagctg ctcgtcgcga tcgccttggc
     2461 accagcagcg agtatcgcct tggcgctgcc ggcatcccac gcattgtaga gcagcagcgg
     2521 cttgcctttc acctgcagct cggcgaagcg cttggccttt tccgtctggt tcattgcatt
     2581 ctcctaagtt gacgagctcg gggacgtggg ctcacccatt ccgtcccttg gaagtgcgga
     2641 cccgaggctt gagcgacttg cgccgcttgt gaccctgcag aatggcgttc gtgacgttct
     2701 atctatcagt aggtccacac gcgttagata aggctggcgc gccggcgcgt caacctctat
     2761 ctatcggtag acagcctgga cgctgcccga tcgccagcgg gcgaccgccc gcagcccata
     2821 aggaagagac cggattgagc tcgtatgaac cggacattct tgcccgcgat ctcgacgtga
     2881 tcttctgcgg cgtgaacccg gccacgactg ccgcagtcgt cggtcacaat ttctccagct
     2941 gcagcaatcg cttctggacg gtgctgcatc tcaccggctt caccgatgtc cgccttcagc
     3001 cgcacgagga gcggcgcttg ctcgagtacc gatgcggcct gaccgccgtc gtcgcccggc
     3061 cgacaagacg ggccatcgat gtgccgccgg acgaattccg gaaagcgcgg ccgggcttcg
     3121 aagccaagat acgtcgctat gcccctcgtt cggtcgcgtt tctcggcaag cgcaccttct
     3181 cggcgatgat ggaccagccg gacgtggcat ggggccggta tcccgcaggg tttgcgggca
     3241 ccacgacctg gatcctgccc aaccccagcg gcctcaacaa agccttctcc ctcgaggcgc
     3301 tggtaaggac tatagcgagt tgcgggcggc cctccgggcc gggccgtcag cgtgagactt
     3361 ccggtcacgg ggagcgacca ggacggattg acagcgcccg gtctcgccgc ggcaacttgg
     3421 atctttcgtg gcgcgttcct tcgatcaccg gagcgatcag gatgaggctc cgacgcaacc
     3481 gtccgctttg gtgaccggca cggtggatgc cggataccgt gccagctttt cggccgggcg
     3541 gatgggacgc gctaccagcc cgccgccgca gttcgggcag cgcccaccca gggtcgtctc
     3601 cgcacagccc cggcagaagg tgcattcgaa gctacagatc atggcgtcgg tggccgccgg
     3661 cggcagatcc ttgttgcagc attcgcagtt ggggcgaagg tctaacacca tggttccttt
     3721 ctgatgacag cgattacgaa tttccgcgcg acgccgtccc cgtctggata tccgggtaat
     3781 actgcggagt ctcgcgtcga tcgaacatgc cgtagtcgcg gaccacgcgc acaatccgat
     3841 ggcggaggcc gccaaaggcg ggcgggctga atttctcggc agagggccgg tcgcgccacg
     3901 acgctaacag cgcgagtttc cctgcgttgt agatgctggc aaaaacgtcg tggtcgatca
     3961 ggtccgcccg atgccgatcg agcgacaacc agtccggcag caccgcgacc gtcatgcctg
     4021 atgacggcgg gacctcggtc agcgtcatga atttcgcccg cccgatctcc gtctcgtcga
     4081 gccgctgttc gacgatcgcc gcgcctgcgg gtggtgccgt gtccgccacg atctcgccca
     4141 cccgcagatg gtagtcctgg aacacctcat cccggccgcg ctgctgggtg ttgtgatgct
     4201 tgccgaccgt ccgccacctc accacggatt tctcgtcgcg ccaggtcgag tgcgacagga
     4261 tccagcccgg ccggcgcgtg ctctcgaagc gttcgttgtc gatgaagccg tctatccctt
     4321 cgaggatcgg tctcaatccc ttcgcaagct cgagatagag gtcgaacttc tcttgcttgg
     4381 gatggacttc gaagatcacc gagaacatcg ccatcgcccc ggattgtcat gcctcatgcc
     4441 gcggagcggc agggcttcct cctctgcgcc agcgggtagc gtctctgacg ccacgctcgc
     4501 cgacgtctct ctcgactggt agggggataa ccgttgtttt ctcccggcgc aaggactatc
     4561 tatcaacaga tagtcatagg agtgactcca tgagtgacgt caaaactgcg atcatggacg
     4621 ccgccgaacg gcgcatgcag cagggcggtt tcggcggctt cagcttccgg gaaatcgcca
     4681 cggatgtcgg catcaagagc tcgagtgtcc actaccattt cccaacaaag gaggatctcg
     4741 cggcggccgt cattcgccgc tgggcggaat atacttccga attgatcgac aaggagctgg
     4801 agaaggaccc agatcccgtc cgtgtctgga cgaaggcctt ccaaggaact gctttctccg
     4861 aagctcacat gtgcccttgt accgtgctcg gcgccgcctc gcag
//
LOCUS       DASUMG010000316         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1165853, whole genome shotgun sequence.
ACCESSION   DASUMG010000316 DASUMG010000000
VERSION     DASUMG010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165853"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="VFX46_05200"
     CDS             complement(<1..166)
                     /locus_tag="VFX46_05200"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005767019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEX5600008.1"
                     /translation="MVNDRSEGLDRVLVLEVGRVTEAAAIAAAKFRGRGNEKAADKAA
                     VDAMRRELNRL"
     gene            complement(208..1533)
                     /locus_tag="VFX46_05205"
     CDS             complement(208..1533)
                     /locus_tag="VFX46_05205"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX5600009.1"
                     /translation="MAPPLKLGIAGLGTVGTGLIQLLDAHAERFRATLGRDITVTAVS
                     ARSRHKDRGFSLDRFRWVDNPVELAADPGIDVFVELIGGDNGIAAEAVEAALRAGKHV
                     VTANKALLARHGVRLARLAEERGIALNFEAAVAGGIPVIKTMREALLGNEISRVYGIL
                     NGTCNYILTTMQEEGRPFAEVLKEAQERGYAEADPTFDIGGFDTAHKLSLLTSLAFGT
                     ELAFEDIHIEGIEHITPADIEAADDLGYRIKLLGVAVRTATGIEQRVHPAMVPKHSAI
                     AEVSGVTNCVAIDGDFVGDLLLVGSGAGSKPTASAVASDIGDIARGLVVPPFLTPASK
                     LKPYTRAMLDRHQGAYYVRLTVYDRPGTMAAIASRMAEQDVSIESIVQRRPRSALPGS
                     GARPVPGSLTPVVLITHETTEEAMRNALAAIERDGKVSEKPQMIRIEKL"
     gene            complement(1542..2768)
                     /locus_tag="VFX46_05210"
     CDS             complement(1542..2768)
                     /locus_tag="VFX46_05210"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX5600010.1"
                     /translation="MTDEFHRIKRLPPYVFSEVNRLKADARAAGADIIDLGMGNPDMP
                     TPKHIIDKLVETVNKPRTNRYSASKGIPGLRRAQAAYYERRFGVKLDPETQVIATLGS
                     KEGFANMAQAITAPGDVILVPNPTYPIHEFGFIISGASIRHIPASNGPEFLRGVERAA
                     KYSIPKPLAMVLSYPSNPTAMTVDLDFYAEAVQLAKRLGLIILSDLAYAEVYFDNNPP
                     PSVLQVPGAIDIAVEFTSLSKTYSMPGWRVGFAVGNERLIAALARVKSYLDYGAFTPV
                     QVAAAAALNGPQDCVEEIRQVYKSRRDTLIESFGRAGWHIPAPPATMFAWAPLPEPYR
                     DIGSVEFAKLLIEKADVAVSPGLGFGEYGEGYVRIALVENEHRIRQAARNIKRFLSQS
                     PESMHNVIPLQQKASG"
     gene            complement(2898..3122)
                     /locus_tag="VFX46_05215"
     CDS             complement(2898..3122)
                     /locus_tag="VFX46_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600011.1"
                     /translation="MAGSAPWAVLLAGVIGLVLAGCSRANSTRLPELATLPEGLLSKE
                     QQQEAVEELQERRKAHRKQAIEEIKSAQRN"
     gene            3336..>4074
                     /locus_tag="VFX46_05220"
     CDS             3336..>4074
                     /locus_tag="VFX46_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEX5600012.1"
                     /translation="MQKPNPENRPVTTAYKLENPEELALNLLKLFEQGGKVMARFVER
                     ADSQVGPYSATNEMMEAAKTLGQVAQFWFSDPGRLAEAQGELVRGYFDLWQSAVRRAL
                     GEKADPVAEPERGDNRFKDPEWSQSPYFDYWKQVYLLTARWAESILEKTDGIDERTRM
                     KAEFYLEQLLSALSPSNFPVTNPEILRETLATNGRNLVQGLAHLAEDMERSGDLFKIR
                     QTDLDAFEVGRNLAVTPGKVVFQNDLLQ"
BASE COUNT          753 a         1281 c         1270 g          770 t
ORIGIN      
        1 ggagccggtt gagctcacgt cgcatcgcgt cgacagcagc tttgtcggcg gccttctcgt
       61 tgccgcgccc tctgaatttg gctgcggcga tggcggcggc ctccgtcacc cgcccaacct
      121 cgaggacgag tacacgatcc aatccctcgc tacggtcgtt gaccatgggg ccttcctcca
      181 tctccggtct cgcccagtcc cgatgtatca gagtttttcg atgcgaatca tctgcggctt
      241 ctctgatacc ttgccgtcgc gctcgatcgc ggcgagcgcg ttgcgcatgg cttcctcggt
      301 cgtctcgtgg gtgatcagga cgacgggcgt cagagagccc ggcaccggcc gcgccccgga
      361 ccccggcagg gccgaacggg gtcgacgctg cacgatgctt tcgatggaaa cgtcttgctc
      421 cgccatacgg ctggcgatcg ccgccatggt tcctggccgg tcgtagacgg tcagtcgcac
      481 gtaataggca ccctgatgcc ggtcgagcat ggcgcgagtg tagggcttga gcttcgatgc
      541 cggtgtcagg aacggcggca caaccagccc gcgagcgatg tcgccgatat cgctggccac
      601 agccgacgcg gtcggcttcg acccggcgcc agagccgacc aatagcaggt ctccaacgaa
      661 atcgccgtcg atcgcgacgc agttggtaac ccccgacacc tcagcgatag ccgagtgctt
      721 cggcaccatc gccggatgga cccgctgctc gatgccagtg gccgtgcgca cggccacgcc
      781 cagcagcttg attcggtagc cgagatcatc tgccgcttcg atgtcggcgg gcgtgatgtg
      841 ctcgatgccc tcgatgtgga tatcctcgaa agccagctcc gtgccgaacg ccaggctggt
      901 cagcagcgac agcttgtggg ccgtgtcgaa gccgccgatg tcgaaggtcg ggtccgcctc
      961 cgcgtagccg cgttcctggg cctccttcag cacctcggcg aaaggccggc cctcctcctg
     1021 catcgtcgtg aggatgtagt tacacgtacc gttgaggatg ccgtatacgc ggctgatctc
     1081 gtttccgagc agcgcctcgc gcatcgtctt gatgacaggt atgccgcccg cgaccgccgc
     1141 ctcgaaattg agagcgatgc cgcgttcctc agccagtcgg gctagccgga cgccatggcg
     1201 agcgagcagc gccttgttcg cggtcaccac atgcttgccg gcccggaggg ctgcctccac
     1261 tgcctcggcg gcaatgccat tatctccgcc gatcaactcg acgaacacat cgatgccagg
     1321 gtcggccgca agctcgacgg gattgtccac ccaccgaaaa cgatcgagag agaagccccg
     1381 gtccttgtga cggctgcgcg ccgacacggc cgtaacggtg atgtcacgcc cgagggtcgc
     1441 cctgaaccgc tcagcatgag catcgagcag ttggatgagg ccggtgccga cggtacccag
     1501 gccggcgatc ccaagtttca agggtggtgc catgtgcgag actaaccgga cgccttctgc
     1561 tgcagtggaa tgacgttgtg catcgactca ggtgactgcg agaggaacct cttgatgttg
     1621 cgcgctgcct gacggatccg gtgctcgttc tccaccaggg cgatgcgcac atagccctcg
     1681 ccgtactcac cgaagccgag cccgggtgag acagccacat ccgccttttc tatcaatagc
     1741 ttcgcaaact caaccgagcc gatgtcgcgg taaggctcgg gaagcggcgc ccaggcgaac
     1801 atggttgccg gcggagcggg aatgtgccag cccgcgcggc caaagctttc gatcagggta
     1861 tcgcggcggc tcttgtacac ctgccgaatc tcttccacgc aatcttgcgg cccgttgagt
     1921 gccgcggcgg ccgcaacttg cactggcgtg aaagcgccat agtcgaggta ggatttgacc
     1981 cgagccagag ccgcgatcaa ccgctcgttt ccaacggcga agccgacgcg ccagccgggc
     2041 atgctgtaag tcttcgagag cgaggtgaac tcgacggcaa tatctatcgc acccggcacc
     2101 tgcagcacgg agggcggcgg attgttgtcg aagtagacct cggcgtaggc gagatccgac
     2161 agaataatga gacccagccg tttggcgagt tgtactgcct ccgcatagaa gtccaggtcg
     2221 accgtcatgg ccgtcgggtt cgacgggtag ctcaacacca tcgccagcgg cttggggatc
     2281 gagtacttgg ccgccctctc gacgccgcgc aggaactccg gcccgttgct cgccgggata
     2341 tgacgaatgg acgctccgga gatgatgaag ccgaactcgt ggatcggata ggtcggattc
     2401 ggcaccagga tgacatcacc cggcgccgtg atcgcctgcg ccatgttggc gaagccttcc
     2461 ttggagccga gcgtggcgat cacctgcgtt tcggggtcga gcttcacgcc gaagcggcgc
     2521 tcgtaatagg cggcttgtgc acgcctgagc cccggaatgc ctttcgaagc cgagtagcgg
     2581 ttggtgcgcg gcttattcac cgtttcgacc agcttgtcga tgatgtgctt cggggtcggc
     2641 atgtccgggt tgcccatgcc gaggtcgatg atgtcggcgc cggcggctcg cgcgtctgcc
     2701 ttgaggcgat tgacctcaga aaagacataa ggtggcaggc gtttgatgcg atggaactcg
     2761 tcggtcacgg gatcaactca tcggttggag atcagcacag acaaatccgc agttcgtctt
     2821 tacgatcgtt tgcttcgcct cgtccagagc cggcaaactg gtcaggtgat gaaacgctcg
     2881 cgtgagaagg cctttggtca gttgcgctgg gcgctcttta tctcttcgat agcctgcttg
     2941 cggtgagcct tccggcgctc ctgaagctcc tcgacggcct cctgctgctg ctccttggag
     3001 agcaggcctt ccggcaacgt cgcgagctct ggcaaccgcg tcgaattggc ccgcgagcag
     3061 cctgcaagca ccagtccaat cacgcccgcg agcagtacgg cccagggcgc ggagccggcc
     3121 aagggccggt tcggtcgaac ctcattattg gaactgcgat gaatcatgca tttcagttta
     3181 atcgatttcg aaagtcttcg cctgtacgct ggtgccggct cgattgagac actatctaaa
     3241 cgtggcgaag tttcaccgat gaattgagac acgctgcgct tccaccggtt ggagccgaac
     3301 gtcacccggg gaagcttccg gacgtgcgga cagcaatgca gaaaccgaac cccgaaaaca
     3361 ggccggtcac cacagcctac aagcttgaga accccgagga gctggcactc aacctgctca
     3421 aactgttcga gcaaggtggc aaggtcatgg ctcgcttcgt cgagcgtgct gacagccagg
     3481 ttggccctta ctcggcaacg aacgaaatga tggaagcggc caagaccctg gggcaggtcg
     3541 ctcagttctg gttcagcgat ccagggcggc tcgccgaggc gcaaggcgag ctggtgaggg
     3601 gctacttcga cctttggcaa tcggccgtcc gccgggcgct cggggagaag gccgatccgg
     3661 tggcagaacc cgagcgcggc gacaaccgct tcaaggaccc ggagtggtcc cagagcccct
     3721 actttgatta ctggaaacag gtctatctgc tgacggcgcg ttgggccgaa tccatcctcg
     3781 agaagacgga cggcatcgac gagcgcacac gcatgaaagc cgagttctac ctcgagcagc
     3841 tgctcagtgc gctgtcgcct tcaaactttc ccgttacgaa cccggagatc ctgcgcgaaa
     3901 ctctggccac gaacggccgc aatctcgttc agggcctcgc ccatttggcc gaggacatgg
     3961 agagatcggg cgatctcttt aagatccgcc agacggatct cgatgctttc gaggtcggcc
     4021 gcaaccttgc cgtgacccct ggcaaagtcg ttttccagaa cgacctcctg cagc
//
LOCUS       DASUMG010000317         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_523516, whole genome shotgun sequence.
ACCESSION   DASUMG010000317 DASUMG010000000
VERSION     DASUMG010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523516"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFX46_05225"
     CDS             complement(<1..412)
                     /locus_tag="VFX46_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011512206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX5600013.1"
                     /translation="MTQSVEELRRESERNREQLTKTVDQLRLQITDTASDIQNKVSPQ
                     HIKSEVSDFISSKTASWIDSLKQHAKENPMQAIAAGTAIAVPVMRMVRGFPLPLLMIG
                     AGLALSSKNVRERAAAAAAPAAEKAQEITGDAMQR"
     gene            complement(610..3138)
                     /locus_tag="VFX46_05230"
     CDS             complement(610..3138)
                     /locus_tag="VFX46_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX5600014.1"
                     /translation="MDKLLVGSEARRKMETADGFVRVRGAREHNLKDVSLDIPRNALV
                     VFTGVSGSGKSSLAFGTLYAEAQRRYLESVSPYARRLFHQMAVPEVDSVEGLPPAVAL
                     QQQRGSPTTRSSVGSVTTLSNLLRMLYSRAGVYPRNQPLLYAESFSPNTPEGACPNCH
                     GIGRVHEVDERSLVPDDTLTIRERAVAAWPSAWQGQNLRDILVTLGYDVDKPWRELSK
                     KDRDWILFTDDQPTVPVYAGYTPAETKRALKRKEEPSYQGTFTGARKYVLQTFATTQS
                     AMMKRRVAQFLSSSDCRACKGKRLKPEPLSVTFAGMDIAEISRLPLKQLRGVLGQHLN
                     GRKRDFADHPEKALVVQRITEDMSARLDVLLDLGLGYLTPERSTPTLSPGELQRLRLA
                     TQVRSNLFGVVYVLDEPSAGLHPADTEALLRALERLKSSGNSLFVVEHELEVVRHADW
                     IVDVGPDAGAQGGQIIYSGPLAGLGGVRNSRTRPYLFDQAVKVAKTPRDPCGWLKLRG
                     VTRNNLDGLDADIPLGIFTSVTGISGSGKSSLISQFLVEAVGGELGHRNEPADDDDGL
                     EAKVQTVGGEITGGLEKIKRLVVVDQKPIGRTPRSNLATYTGLLDHVRKLFAATKQAR
                     ARRYDAGRFSFNVAKGRCGTCLGEGFICVELLFLPSVYAPCPTCKGARYNTKTLEIKI
                     RDRSIADVLAMTVDEAFEFFANDDPLRRSLGVLREVGLGYIRLGQSATELSGGEAQRV
                     KLATELQRAQRGETLYVLDEPTTGLHPSDVAKLMKQLDRLVESGNTVVVVEHDMTVAA
                     VSDWVIDIGPGAGDEGGRIVASGAPAQVARETQSRTAKYLAAVI"
BASE COUNT          624 a         1079 c          974 g          698 t
ORIGIN      
        1 cgcgctgcat tgcgtctccc gtaatttcct gagccttttc cgccgctggc gcagcggctg
       61 ctgccgcgcg ctcacgcacg ttctttgaac tcaatgcaag ccccgcccca atcatcaaaa
      121 gcggcagcgg aaagccacgc accatccgca tcaccggcac cgcaatggcg gtccctgccg
      181 cgatcgcttg catcggattt tccttggcat gctgtttcag tgaatcgatc cagcttgcag
      241 ttttggagct aatgaaatca gatacttcag acttgatgtg ctgcggggaa accttgtttt
      301 ggatgtcgga agcggtgtcg gtaatctgca acctcaactg atcgactgtt ttcgtcagtt
      361 gttctcggtt gcgctcagat tcccgtcgca attcctcaac ggattgtgtc atcgtgtcac
      421 cctctgggat ttctttcggg acgaactcgc gctgttttga atcgttccta accggaacga
      481 agtcacctaa ctttagtttg cagagccaag tgggttattg gacgccgatg tggctcccgg
      541 tctctcgcca cgaaaagcgc ggaactgccg gttatgcgag actttgccgg atgccttgtg
      601 cagcactctt caaataacag cagctaggta tttcgccgtt cgactctgcg tttccctcgc
      661 gacctgcgct ggcgcccctg aagccacgat ccggccgccc tcgtcgcccg ctccgggccc
      721 gatgtcgatg acccagtcgc tgaccgcggc aacggtcatg tcatgttcga ccaccacaac
      781 cgtgttgccg ctctcgacga ggcggtccag ttgtttcatc agtttcgcca cgtccgacgg
      841 gtgcagcccc gtcgtcggct catcaagcac gtagagcgtc tcgcctcgct gcgcgcgctg
      901 gagctcggtg gcaagcttga cgcgttgggc ctccccaccc gacagctcgg tggccgattg
      961 ccccagccgg atgtatccca gaccgacttc acgaagcacg cctagagatc gtcgcagcgg
     1021 atcgtcgttg gcaaagaatt caaatgcttc gtcgacggtc atggccagca cgtccgcgat
     1081 cgacctatcc cggatcttga tctccagcgt cttggtattg tagcgggcgc ccttgcacgt
     1141 ggggcacggc gcgtacacgc tcggcagaaa gagcagttca acgcaaataa acccttcgcc
     1201 caggcatgtc ccacaacggc ccttggccac attgaaggag aacctgcccg cgtcgtaccg
     1261 cctggcacga gcctgcttgg tcgcggcgaa gagcttccgg acatgatcca gcagaccagt
     1321 gtacgtcgcc aggttcgacc ggggcgtccg gccgatcggc ttctgatcga cgaccaccag
     1381 ccgtttgatt ttttccagac cgccggtgat ctcgccgcct actgtctgga ccttggcttc
     1441 cagcccgtca tcatcatcgg ccggctcatt gcggtgaccc aattcgccgc cgacggcctc
     1501 gacgaggaat tggctgatga ggcttgactt gcccgaccct gaaatacctg taacgcttgt
     1561 gaagatgccc aatggaatat cggcatccaa accatcaaga ttgttgcgcg tgacgccgcg
     1621 cagcttgagc caaccgcagg ggtctcgggg cgtcttggca accttaacgg cctggtcgaa
     1681 gagataaggt cgtgtacggg agttccgcac gcctccgagg cccgccaacg ggccgctgta
     1741 gatgatctgg ccgccctgcg cgccggcgtc gggaccgacg tcgacaatcc agtctgcatg
     1801 tcgcacgacc tcgagttcgt gctcgacgac gaagagcgaa tttccggacg actttaggcg
     1861 ttccagagcg cgcagcagcg cctcggtatc cgccgggtgt agtcctgccg aaggctcgtc
     1921 caggacgtat acgaccccga acagattgga tcgcacttgc gtagccaacc gaagccgctg
     1981 aagctccccc ggcgacaacg tcggcgtact gcgctcgggc gtcagatagc cgagacccag
     2041 gtcgagcagt acgtcgagac gtgccgacat gtcttctgtg atgcgctgca cgaccagagc
     2101 cttctcggga tggtcggcga agtccctttt tcggccattg aggtgctggc cgagcacgcc
     2161 gcggagctgc ttcaacggta atcgcgaaat ttccgcgata tccattccgg cgaaagtgac
     2221 cgagagaggc tcgggcttga gccgcttgcc tttgcacgcc cggcaatctg agcttgaaag
     2281 aaactgcgcc acgcggcgct tcatcatcgc gctttgcgtc gtggcaaagg tttgcagcac
     2341 gtattttcgc gcgccagtaa aggtgccctg atagctcggc tcctcctttc gcttcagcgc
     2401 ccgcttggtc tcggccggcg tgtatcccgc gtaaaccggc acagtcggct gatcgtcggt
     2461 gaacaggatc cagtcccgat cctttttcga taattcgcgc cacggcttgt ctacgtcgta
     2521 gccaagcgtc accagaatgt ccctcaggtt ctggccctgc catgcgctcg gccacgccgc
     2581 gacggctcgc tcccggatgg tcaacgtgtc gtcgggcacc agcgaacgct cgtcgacctc
     2641 atggacgcgg ccgatgccgt ggcaattcgg acaggcgccc tcgggcgtgt tcggcgaaaa
     2701 cgactcggcg taaagaagtg gctgatttcg cggatagacg ccggcgcgcg agtagagcat
     2761 ccgcagcaga ttggacaacg tcgtcacgct gccgaccgac gatcttgttg tcggcgagcc
     2821 gcgctgctgc tgcagggcca ccgccggagg tagcccctcg accgaatcca cctcgggaac
     2881 cgccatctgg tggaaaaggc gccgagcgta gggcgagacg gattcgagat aacgccgctg
     2941 ggcttctgca tacaacgtcc cgaaggccag ggacgacttc ccggatccgg acacgccggt
     3001 aaaaacgacc agcgcattac gcggaatatc cagcgacacg tccttgagat tgtgttcgcg
     3061 ggctccgcga acccgcacga agccgtccgc ggtctccatc ttccgcctcg cttcactgcc
     3121 gaccagcaac ttatccatcc aacctcctcg ccgcgcacgc aggcgcgtcg cgttcaatag
     3181 gaaagtcttc gagaattgac gggttccgcg ggcggtggcc ctcctgcacc gacgtcctcg
     3241 tgaggtcccg ttcgagattt cgcatattgg aacccgccct ttcgcccgtt gttctagttc
     3301 gagcgaagca gcggagattt tgatgaataa ccgaggtcgg cagatctaca aaccggagtt
     3361 tcgcttcggc ctggg
//
LOCUS       DASUMG010000318         3683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_523822, whole genome shotgun sequence.
ACCESSION   DASUMG010000318 DASUMG010000000
VERSION     DASUMG010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523822"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2458)
                     /gene="acnA"
                     /locus_tag="VFX46_05235"
     CDS             complement(<1..2458)
                     /gene="acnA"
                     /locus_tag="VFX46_05235"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX5600015.1"
                     /translation="MTAQEPVSLDSFKSRKTLTVDNKTYTYFSLPDAERNGLNGISRL
                     PYSMKVLLENLLRHENGRSVTKDDIAAISAWLDNRGKVEKEIGFRPARVLMQDFTGVP
                     AVVDLAAMRDGMQALGGDPRKINPLVPVDLVIDHSVIVDEFGTPRAFARNVELEYQRN
                     LERYEFLKWGQGAFSNFRVVPPGTGICHQVNLEYLAQTVWTNEVGGETYAYPDTLVGT
                     DSHTTMVNGLAVLGWGVGGIEAEAAMLGQPLSMLIPEVVGFKLTGRLREGVTATDLVL
                     TVTQMLRKHGVVGRFVEFFGPGLDHMSIADRATIANMAPEYGATCGFFPIDQATLEYL
                     ELTGRDAHRIALVEAYAKAQGLFRTADSPDPVFTETLELDLGTVEPSMAGPKRPEGRI
                     ALEKVASGFATALETEYRKTSNTSARYKVPGRDFDLGHGDVVIAAITSCTNTSNPSVL
                     IGAGLLARNAVEKGLTSKPWVKTSLAPGSQVVAAYLEKSGLQPYLDKLGFNLVGFGCT
                     TCIGNSGPLAPEISQTINENGIVAAAVLSGNRNFEGRVSPDVQANYLASPPLVVAYAL
                     AGTVTKDLTKEPLGIGKDGNPVYLRDIWPSNQEIQKYIAENISRELFKQRYADVFKGD
                     EHWQKIKATPGLTYSWNSGSTYVQNPPYFQGITKEPQPISDIENARILALLGDKITTD
                     HISPAGSIKKDSPAGRYLLERQVRPEDFNQYGTRRGNHEVMMRGTFANIRIKNHMVKD
                     ENGNIREGGYTVHYPSGETMFIYDAAMRYREEGVPLVVFAGVEYGNGSSRDWAAKGTR
                     LLGVRAVITQSFERIHRSNLV"
     gene            2693..3295
                     /gene="ccmA"
                     /locus_tag="VFX46_05240"
     CDS             2693..3295
                     /gene="ccmA"
                     /locus_tag="VFX46_05240"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEX5600016.1"
                     /translation="MSNLTVERGGRHVISELSFEVAAGEALLLTGPNGAGKTTLLKTV
                     AGFISPAGGRVTLRGVSDDEATAEQIHYLGHLNGLRPTLTVEENARFWASYLGGTDAD
                     VSTALERFGLGSLATIPAGYLSAGQKRRLALARLLLAPRPLWLLDEPSSSLDAHSTEV
                     LASVLDEHVAAGGLIIAATHLPLGLRQSRELRLGFAARAA"
     gene            3313..>3683
                     /locus_tag="VFX46_05245"
     CDS             3313..>3683
                     /locus_tag="VFX46_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX5600017.1"
                     /translation="MKRDLLIAAREGGATGTALGFYLIVIALMPLGLGPDLQLLSRIA
                     PGVLWIGLLLAALLSLGRIFETDKEEGVLEILATGRVPLEFVVAAKAFAHWLSSCVPL
                     AILSPLLGLLLNLELAAYPILL"
BASE COUNT          606 a         1158 c         1190 g          729 t
ORIGIN      
        1 cgaccagatt cgagcggtga atgcgctcga agctctgggt gatgaccgcg cggacgccga
       61 gaaggcgcgt gcccttggca gcccagtcgc gcgaggagcc gttgccgtac tcgacgccgg
      121 cgaagacgac cagcggcacg ccctcctcgc ggtaacgcat ggccgcatcg tagatgaaca
      181 tcgtctcgcc cgagggataa tggacagtgt agccgccctc gcggatgttc ccgttctcgt
      241 ccttcaccat gtggttcttg atgcggatgt tggcgaaggt gccccgcatc atcacctcat
      301 ggttaccgcg gcgcgtgccg tactggttga agtcctcggg ccgcacctgc cgctccagca
      361 agtagcgtcc cgccgggctg tccttcttga tggagccggc aggcgagatg tggtcggtag
      421 tgatcttgtc accgaggagc gccaggatcc gcgcattctc aatgtcggag atcggctgcg
      481 gctctttcgt gatgccctgg aagtagggcg ggttctgcac gtaggtcgag ccgctgttcc
      541 aggagtaggt cagtccgggc gtggccttga tcttctgcca gtgctcgtcg cccttgaaca
      601 cgtcggcgta acgctgcttg aacagctcac gcgagatgtt ctcggcgatg tacttctgga
      661 tctcctggtt cgacggccag atgtcgcgca ggtagacggg gttgccgtcc ttgccgatgc
      721 cgagcggctc cttcgtcagg tctttggtga ccgtgccggc gagagcgtag gcaacgacca
      781 gcggcggtga cgcgaggtag ttcgcctgca cgtcggggct cacgcggcct tcgaagttgc
      841 ggttgcccga aagcacggcc gccgcaacga taccgttctc gttgattgtc tgcgagatct
      901 ccggcgccag cggccccgag ttgccgatgc aggtggtgca gccgaagccg accagattga
      961 agccgagctt gtcgagatac ggctgcaggc cggacttctc gagataggcg gccacaacct
     1021 gcgacccagg cgccagcgag gtcttcaccc acggcttgga ggtcaggccc ttctcgacgg
     1081 cattgcgcgc gagcaggccg gcaccgatga gcacgctcgg gttcgatgtg ttggtgcagc
     1141 tggtgatggc tgcgatgacg acgtcgccgt ggccaaggtc gaaatccctg ccgggtacct
     1201 tatagcgggc actggtgttg ctggtcttgc ggtactcggt ctcgagcgcc gtcgcaaagc
     1261 cgcttgccac cttctcgagc gcaatccgcc cttccggccg cttcggacca gccatggacg
     1321 gctctacagt gccaaggtcg agctccagcg tctccgtgaa caccggatcg gggctgtccg
     1381 ccgtccggaa caggccttgg gccttcgcgt aggcctcgac gagtgcgatc cggtgggcgt
     1441 cgcggcccgt cagctccagg tactccagcg tggcctggtc gatcgggaag aagccgcagg
     1501 tcgcgccata ctccggtgcc atgttggcga tggtggcccg gtccgcgatg gacatgtgat
     1561 caaggcctgg gccgaagaac tcgacgaacc tgccgacgac gccgtgctta cgcagcatct
     1621 gcgtcacggt gagcacaagg tccgtcgccg tgacaccttc cctcaggcga cccgtcagtt
     1681 tgaagccgac aacctcggga atgagcatcg acagaggctg accgagcatc gctgcctccg
     1741 cctcgatgcc acccacaccc cagccgagaa ccgcgaggcc attgaccatc gtcgtgtggc
     1801 tgtccgtgcc gacgagtgtg tccgggtaag cgtaggtttc gccgccgacc tcattggtcc
     1861 aaacggtctg cgccagatac tcgaggttaa cctggtggca aatgccggtg cccggcggaa
     1921 ccacgcggaa gttggagaac gcgccctgcc cccacttcag gaactcgtag cgctccaggt
     1981 tccgctggta ctccaattca acgttgcggg cgaaggcgcg cggcgtgccg aactcgtcca
     2041 cgatcaccga gtggtcgatg acgagatcga cggggacgag cgggttgatc ttccgcggat
     2101 cgccgcccag cgcctgcatg ccatcgcgca tggccgcgag gtccacgacc gctggcacgc
     2161 ccgtgaagtc ctgcatcagc acgcgggcgg gccggaagcc gatctccttc tcgaccttgc
     2221 cgcgattgtc gagccaggcc gaaatggctg cgatgtcgtc tttcgttacc gagcggccgt
     2281 tctcatggcg gagaaggttc tccagcagca ccttcatcga gtacggcaaa cgggaaatgc
     2341 cattgaggcc attccgctcc gcatcgggca gcgagaaata ggtgtaggtc ttgttgtcga
     2401 cggtgagtgt cttgcggctc ttgaagctgt ccaacgacac gggttcttgc gcggtcacgt
     2461 tctgtcctcc atccgagcaa ttacagccgc gccgccggag cgccggcgga gggaacaatc
     2521 ccgtttttgc gctagcgtcg atagaagcgt gctccttata tatccgcgcc cgggcgagga
     2581 ctagtccacg cgttgtcccg gcgccgatgg cgagccatca gccctgagac aggtttctgt
     2641 gaccgacaaa gagcatcgca ctaacgaaaa cgcgagcagg cctcgcctca tcgtgagcaa
     2701 cctgacggtt gagcgcggcg gccggcatgt gatttctgag ctttcattcg aggtcgcggc
     2761 gggagaagct cttttgctca ccgggccgaa cggtgccggc aagacgaccc tcttgaagac
     2821 ggttgcaggc ttcatttcac ccgcaggtgg tcgtgtgaca ctgaggggtg tgagcgacga
     2881 tgaggcaacc gcggagcaaa ttcactacct ggggcatttg aatgggctac gcccgacgct
     2941 caccgtcgag gagaacgccc gcttctgggc gagctacctt ggtggcactg acgccgacgt
     3001 atcgacggct ctcgagcggt ttggcctcgg atcacttgcc accatccccg cagggtatct
     3061 ttcagccggg cagaagcgcc ggcttgcact tgccagactg cttctcgcgc cgcgtccgct
     3121 ttggctgctc gacgaaccgt cctcctcatt ggatgcccat tcgactgagg tgctcgccag
     3181 cgtgttggat gagcatgttg cggccggcgg gctgatcatc gctgcaaccc atttgccgct
     3241 cgggcttcgg cagtcgcgtg agctgcggct cggttttgcc gcgagggccg catgagcgcc
     3301 ctctggtctc tcgtgaagcg cgacctgctg attgctgccc gcgaaggtgg cgcgacagga
     3361 acggcgctcg gcttctacct catcgtcatc gccctgatgc cgctcgggct gggcccagac
     3421 ctgcaactgc tctcgcggat tgcacccggc gtgctgtgga tcggacttct ccttgccgcg
     3481 ctgctgtcgc tcggccgcat ctttgaaacg gataaagagg aaggcgttct ggagatcctg
     3541 gcgaccggtc gcgtgccgtt agagttcgtg gtggcggcca aggcgttcgc ccactggctg
     3601 tcgagctgcg tgccgctagc aatcctgtcc cccctgctcg ggctgctgct caatttggag
     3661 cttgcggcct atccaattct gct
//
LOCUS       DASUMG010000319         2322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_202882, whole genome shotgun sequence.
ACCESSION   DASUMG010000319 DASUMG010000000
VERSION     DASUMG010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202882"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..780)
                     /locus_tag="VFX46_05250"
     CDS             complement(1..780)
                     /locus_tag="VFX46_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018689850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEX5600018.1"
                     /translation="MAQFDNRYAQAATAARTGVAVDEGLRAYMLSVYNYMAAGVALTG
                     VVAYMLFSAAVTGDPGSAVARLGNGTMLTEFGRLLYVSPLRWVIMLAPLAFVFFLSFR
                     VYQMSVSAAQIAFWLFAGVMGASMSTIFLVYTGASITRVFFITAAAFASLSLWGYTTR
                     KDLSGWGTFLFMGLIGIIIAALVNLFLQSSALQFAVSVMGVLVFAGLTAYDTQRIKDG
                     YYAVAGSAELAAKTAIMGALSLYLDFINMFTSLLYLFGERE"
     gene            complement(959..>2322)
                     /locus_tag="VFX46_05255"
     CDS             complement(959..>2322)
                     /locus_tag="VFX46_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5600019.1"
                     /translation="APEQMRPRSRVIVLTVAAIAALVLLAVLSSESRWIAIYFCLGLT
                     AVFALFTGLGKLIAGLARRAPRSRIPEIALAIGNLGGPGGLTRSTVLSLGAGLSLLVA
                     VALTDHSMVRELTTHLPETSPTYFVLDIRRDDAARFRELVDRVAPGSEIRDAPMLRGR
                     LVQLGGRPVDQIRPPPEAQWVLSGDRGLTYSETVPEGSRIVAGEWWPQNYDGEPLVSF
                     EAELAGHLGIGLGDTVTVNVLGRNITARIANLREVQWESLAINFVMVFSPNALRAAPH
                     NLLATITLPPTADLTTDAELARAISNTFPSVTAIRVKDAIDAFSEVFEKVMTAVRAAG
                     SITLLAGALVLAGALATAQRRRIKQAVILKTLGAVRRRILLSHFVEYAVLAAAVAAVA
                     ALLGTVAAWVVVTQVMEIDFTFSSTAVAGALGLALLLVMGFGGFGTWQVLRAPAVPYL
                     RSD"
BASE COUNT          514 a          724 c          719 g          365 t
ORIGIN      
        1 tcactcgcgc tcgccgaaca ggtagagcag cgaggtgaac atgttgatga agtcgagata
       61 gaggctcaga gcacccatga tggccgtttt cgcagccagc tcggcgctac cggcaaccgc
      121 gtagtagccg tccttgatcc gctgggtgtc ataggccgtc agaccagcga acaccagcac
      181 gcccatcacg gaaaccgcga actgcagcgc gctcgactgc aggaagaggt tgaccagcgc
      241 ggcaatgatg atgccgatca ggcccatgaa caggaaggtg ccccaaccgg acaggtcctt
      301 gcgggtcgtg tagccccaca ggctaaggct ggcgaacgcg gcggccgtga tgaagaacac
      361 gcgggtgatc gaggcgccgg tgtagacgag gaagatcgtc gacatcgatg cgcccatcac
      421 accagcaaac agccagaacg cgatttgcgc ggcgctgacg ctcatctgat agacgcggaa
      481 ggacaggaag aacacgaagg ccaacggagc cagcatgatc acccagcgca gcgggctgac
      541 atacagcagc cggccgaact ccgtcagcat ggtgccattg cccaaccggg cgacagcgga
      601 gccgggatcg cccgtgacgg cggcgctgaa caacatgtag gcgaccacgc cagtaagggc
      661 cacgccggcc gccatgtagt tgtagacgga tagcatgtac gcgcggaggc cctcgtcgac
      721 cgctacgccc gtacgggcgg cggtggcggc ttgcgcatac ctgttgtcga attgagccat
      781 tgtcgatcct ttggtcgctg attgcgagca cccaaaaact cagtggcttg caatatgacc
      841 cgcacgcgcc tcgctttcaa ggcagcgcgt acccgtcata ccgcgactgg gtgaaattat
      901 tggctgagat ccctggacgc aagctcaatg gacaggccaa tgttgctcaa gcggctcctc
      961 aatcagaccg caaataggga acggccggcg cccgcagcac ttgccaggtg ccgaatccac
     1021 caaaacccat cacgagcagc aatgccaggc ccagggcgcc cgcgaccgct gttgacgaaa
     1081 atgtgaagtc gatctccata acctgggtca ccacgaccca ggcagcgacg gtgccgagca
     1141 atgcagccac cgctgcaacg gcggcggcca gaacggcgta ctccacgaaa tgggaaagga
     1201 gtatccgtcg tcggacagct cccagcgtct tcagaatgac cgcttgcttg atgcgccgcc
     1261 gctgcgctgt tgctagcgct cccgccaata cgagagcacc cgcaagcaag gtgatgctgc
     1321 cagccgcgcg caccgcggtc atgaccttct cgaagacctc gctaaaggca tcgattgcat
     1381 ctttgacgcg gatcgccgtc acggaaggaa aagtgttcga gatggcgcgg gcgagctccg
     1441 cgtccgtggt taggtcagca gtgggcggca atgtgatcgt tgccaaaaga ttgtgcggcg
     1501 cagcccgcag cgcattgggg gaaaacacca tcacaaagtt gatggcaaga gattcccact
     1561 gcacctcccg taagttggcg atgcgcgcgg tgatgttgcg gccgagcacg ttgacggtaa
     1621 ccgtatcgcc gaggccaatc ccgaggtgcc ccgcgagctc cgcctcgaac gaaaccaacg
     1681 gctcgccgtc gtaattctgc ggccaccatt ccccggccac gatccgcgaa ccttcgggca
     1741 cagtctcgga gtaagtcagc cctctgtctc cgctcagcac ccattgggcc tcgggtggcg
     1801 gcctgatctg atcgaccggc ctgccaccaa gctgcacgag gcgacctctg agcataggcg
     1861 catcgcgtat ttcagagccg ggcgccacgc ggtcgacgag ctcgcggaac cgcgctgcgt
     1921 cgtccctgcg aatgtcgagc acgaagtacg tggggctcgt ttcgggaaga tgggtcgtta
     1981 gctcgcgaac catcgaatgg tccgtcaaag cgactgccac cagcaacgac aaccctgcgc
     2041 cgagcgacag tacggtcgaa cgggtgagac cgccgggccc gcccaggttc ccgatggcaa
     2101 gtgcgatttc gggaattcga gagcgcggag cacgtcgcgc gagaccagct atgagctttc
     2161 caagtccggt gaaaagcgcg aatacggccg tcagccccag gcagaagtaa atcgcgatcc
     2221 agcgcgattc tgacgacaag actgcaagca ggacgagcgc ggcgattgcg gccaccgtca
     2281 atacgatgac tcgcgatcga ggtcgcattt gctcaggcgc ta
//
LOCUS       DASUMG010000320         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_844733, whole genome shotgun sequence.
ACCESSION   DASUMG010000320 DASUMG010000000
VERSION     DASUMG010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844733"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VFX46_05260"
     CDS             complement(<1..242)
                     /locus_tag="VFX46_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600020.1"
                     /translation="MAYIPVSENGALAACRWQFGPRTVGGYLTAYGLAIVAPILLFSG
                     ALLHGYISIENRQLERQVLQTARLLTLDVDRELSGL"
     gene            928..>2574
                     /locus_tag="VFX46_05265"
     CDS             928..>2574
                     /locus_tag="VFX46_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX5600021.1"
                     /translation="MDLIRYKQPHSGNAPPLLLALVIGLIIAVLYFGGELFVPLAVAV
                     LLSFVLTPLVAFLRKLRVPRVPAVILVVAIAFSTIFSIGAIMGAQLADLAGQLPKYET
                     TLRQKIRDLRPQPGAPAGVLERATDALEKLGEELDREPQRRSPTGSSTSEPIPVEIHH
                     PPPRALEYYQRVVTPLLGPTAQTGLILILVIFILLQREDLRDRLIRVFGGEDFERTTA
                     AMSDAAGRLSRFFLTLTGMNIVYGAVIAAVLWLLGIPGPILWGILAGLMRFVPYIGSI
                     IAAVFPVLLAAAVDPGWSLVLVTLLVYVIGEFTMGQVMEPWLLGSSTGLTPVAVVAAA
                     SFWTWLWGPVGLLLAIPLTVCLIVLGRHVPQLNFIYVLLGDQPALTPAQRFYQRMLAG
                     DIDEITFEAEQLLKKQSLVTYFDEVALPALVMAQEDTRRGRFDHNRLAAMRAFVEELI
                     DDLSDFEVETPERNEDPEAPKPPPDLPVLTADELKRGWQVATPVLSIGIRNPLDRAAA
                     TILAHLATKHGIETRVLGAEDVTPSKVDSLDLGGAQLAVLS"
BASE COUNT          485 a          807 c          732 g          550 t
ORIGIN      
        1 attaagccgc tgagctcgcg atcaacgtcc agagtcagca aacgggcggt ctgaaggacc
       61 tgccgctcca attgccgatt ctcgatcgaa atgtagccgt ggagaagggc accagaaaaa
      121 agcaatatcg gcgcgacgat cgccaagcca tatgccgtga gatatccgcc gactgtccgc
      181 ggcccgaatt gccaacggca ggccgcgagt gccccgtttt ccgacacggg aatgtaggcc
      241 atttccccct ctgatcgagc tttacccggt attgaggtaa gtcccgcggc ggtccccccc
      301 atttcggagc atacgcataa cggatccacg catcctcgat ccgcggcgag gcgatgactt
      361 gtagtcagtc gcgcacattg ccgcagcctc gccggaaatg acgggattcg gctcccaacc
      421 gaacggggcc acccacaacc cggcttcatc cacaacttgg gcgccctcgt ctcgatggcg
      481 aggctcgccg gtcacggcgt gtcgcgggag tgttacgcgc aatgtcacaa tattaaccct
      541 cactgccagc ccgtaacgcg cgaccggttt attggttcca tcgctaatca tttaagttct
      601 tgtgagcata atcaggtttc acttctgcaa cacttgtgag aactccctgt ttggaaccgt
      661 tttcagtttg cacgcgtttg atttgtgccc ctagacgtgg acgcaagcga agcgtccaat
      721 gcctcgaccg cggtcgcatg ccggccgcgg tctccttttc tcaaatcgtc ttctttctcg
      781 atcttgtcga tccccgggat gaagctagac ggccccgttc ttcaaagtat cgggcccgag
      841 aacgcaaagg cctgccatcg cgttccttga gcacgtaagc cctagcccgg ccggtcgctc
      901 ggccgccata tcgagcacgg aactgcaatg gacctgatcc ggtacaaaca gcctcactcc
      961 ggcaatgcac caccacttct gctcgctctg gtcattggtc tgatcattgc ggtgctttac
     1021 ttcggcggtg agctatttgt gccactcgcg gtagccgtgc tgctgagctt cgtattgacg
     1081 cctctcgtgg ccttcctacg aaagctccgg gtgccgcgcg ttccagcggt catcctagtg
     1141 gttgcaatcg ccttttcgac cattttctcg attggcgcga tcatgggtgc tcagctggcg
     1201 gatctcgccg gacagctgcc caagtatgaa acgacgctaa gacagaagat ccgagatttg
     1261 aggccacagc caggtgcacc ggccggagtc ttagagcgag cgacggacgc gctcgagaaa
     1321 ctcggcgaag agctggatcg ggagccgcaa cggagaagcc caactgggtc ttcaacctct
     1381 gagccaatac cggttgaaat ccaccacccg ccgccgcgcg cattggagta ctaccaaagg
     1441 gttgtcactc ctctcttagg gccgacggcc caaaccggac ttatcctcat cctggtcatc
     1501 ttcatccttc tccagaggga ggacctgcga gaccggctga tccgcgtttt cggaggcgag
     1561 gatttcgagc ggacaaccgc tgcgatgagc gatgccgcag gccgcctcag tcgctttttc
     1621 ctgacgctta ccgggatgaa tatcgtctat ggcgctgtaa tcgcggcagt gctttggctc
     1681 cttggaattc ctggcccgat cctttggggc atcttggcgg ggttgatgcg cttcgttcct
     1741 tacatcggat cgatcatcgc agcggtcttc ccggttctcc tcgccgcagc ggtcgatccc
     1801 ggatggtcgc tggtcctcgt gacgctgctc gtttacgtca ttggcgagtt cacgatgggg
     1861 caggtcatgg agccctggct gctaggcagc tccaccggcc ttacgcccgt ggccgttgtc
     1921 gcagcggcct cgttctggac atggctctgg ggaccggtcg gtctgctgct ggcaattccg
     1981 ttgacggtct gtctcatcgt cctcggtcgg catgtgccgc agctcaattt catctacgta
     2041 cttttgggcg atcagccggc gctgacgccg gcacagcgct tctaccagcg catgctggcc
     2101 ggcgacatcg acgagatcac attcgaggcg gagcagcttc tcaagaagca atcgctcgtc
     2161 acctacttcg acgaggtcgc gcttcccgct cttgttatgg cacaggagga cacccgccgc
     2221 gggcgcttcg accacaaccg cctcgcagcg atgcgcgcat tcgtcgagga gctgatagac
     2281 gatctgagcg acttcgaggt cgagacgccg gagaggaatg aagacccgga agcacccaaa
     2341 ccgcctcccg atttgccggt gctgacagcc gacgaactga agcgaggctg gcaggtggcc
     2401 acgccggtgc tgtcaatcgg gatccgcaat ccgctcgacc gagccgcagc aactattttg
     2461 gctcatcttg caaccaagca cggaatcgaa acacgcgtgc ttggtgcgga ggacgtcacc
     2521 ccatcgaagg tcgattcgct cgacttaggc ggcgctcaac tggccgtgct ctcc
//
LOCUS       DASUMG010000321         2969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_844810, whole genome shotgun sequence.
ACCESSION   DASUMG010000321 DASUMG010000000
VERSION     DASUMG010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844810"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFX46_05270"
     CDS             <1..301
                     /locus_tag="VFX46_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX5600022.1"
                     /translation="VKREFLPGPEVQSDEDLMAFARAKGTTIFHPCGTCKMGRDPMAV
                     VDERLRVHGISGLRVIDASIMPTMTSGNTNAPTIMIAEKASDMILEDAHQHAVAV"
     gene            312..1142
                     /locus_tag="VFX46_05275"
     CDS             312..1142
                     /locus_tag="VFX46_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600023.1"
                     /translation="MTKIDYEAEYNNRRRVPEHTEIMARWAERSARVRSTVRCELDQP
                     YGPGPRNCYDLFLPAGEAANNAPLVVYIHGGYWQRGDRADYSFVAEVLVGKGAAVALP
                     SYTLCPEATVAQIIEEMRQFVRTIWTRMNRRPVIVGHSAGGHLAAALLATDWVKYDLP
                     SDVIRAAYSISGVFALEPLIPTSLNEALRLTPEAARESSPILWPPPPADRVFVAAVGG
                     AESQEFLRQSLEITAAWSAAGVKAECIVIPHANHFTVVDELTRPESAMVFRVLQLAQQ
                     "
     gene            1287..1667
                     /locus_tag="VFX46_05280"
     CDS             1287..1667
                     /locus_tag="VFX46_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600024.1"
                     /translation="MTIVLMVMLAIASPLAHVVLPQALANQSFVHGGSKHAHPHHHGD
                     ELPIAALGAFGENSSHEACWPESNCAKLHEFCPDCSYFCHPVFDVTGEYLIENQGRPV
                     AYSAPNAILKGVIPDVLPRPPQIG"
     gene            1760..2203
                     /locus_tag="VFX46_05285"
     CDS             1760..2203
                     /locus_tag="VFX46_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX5600025.1"
                     /translation="MKSYLPSLATIFAIAFAATAAPAAAQSADSHGGHGHGEASMHST
                     EHGHGHGSHSALPIKGTVNEVSKAYAEANAKMHAGMDIEFTGEADVDFVRGMIPHHQG
                     AVDMARIVLQYGKDPEIRKLAGEIIKAQEAEISMMQEWLKKRGLQ"
     gene            complement(2236..>2969)
                     /gene="ubiG"
                     /locus_tag="VFX46_05290"
     CDS             complement(2236..>2969)
                     /gene="ubiG"
                     /locus_tag="VFX46_05290"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEX5600026.1"
                     /translation="DPQEIDRFAQLAGKWWDPEGEFRPLHKIGPARLSFIRSHLVHHF
                     GLPESSAKSLSGLSILDVGCGGGLISEPLARLGARVTGIDPAKENIAAAIRHAEPQGL
                     DIDYRACRVEDLVDEQRQFDAVVCLEVIEHVPDPQAFIAACAALVRPGGIFIASTINR
                     TLKAYALAIIGAEYILRWLPVGTHQWDRLVTPDELARYCTSAGLQQPTFEGLIYNPLT
                     GRWTLGADIDVNYMAAAAKPAPASH"
BASE COUNT          593 a          892 c          922 g          562 t
ORIGIN      
        1 tgtgaagcgc gaattcctgc ctggtcccga ggtgcagagc gacgaagact tgatggcgtt
       61 tgccagagcc aagggcacga cgattttcca tccgtgcggc acctgcaaga tggggcgcga
      121 ccccatggcg gtggtcgatg agcgtctgag ggtgcacggg atttccggcc tgcgggtcat
      181 cgacgcgtcg atcatgccga ccatgacttc gggcaatacc aacgcgccga cgatcatgat
      241 cgcggaaaag gcatccgaca tgatcctgga ggatgcgcac cagcacgcgg tggcagtcta
      301 gggaggggca cgtgacgaag atcgattacg aggccgaata caacaaccgg cggcgagtgc
      361 ccgagcacac ggagatcatg gcgcgctggg cggagcggtc agcccgggtc cgttcgacgg
      421 tgagatgcga gctggatcag ccttatggtc cggggcctcg caactgctat gacctatttc
      481 ttccggctgg agaggcggcc aacaacgcgc cgctggtcgt ctatatccac ggcggctatt
      541 ggcagcgcgg cgaccgggcg gactacagct tcgtggccga ggtgctggtc ggcaagggtg
      601 ccgcggtcgc gcttccctca tacacgttgt gccctgaagc caccgtcgcg cagatcatag
      661 aggagatgcg ccagttcgtg cgcacgatct ggacgcgcat gaaccggcgg ccggtgatcg
      721 ttggccattc cgctggcggt cacttggctg cagctttact ggccacggac tgggtgaaat
      781 atgatctgcc gtcggatgta atccgggcag cctattcgat cagcggcgtc ttcgcgctcg
      841 agccgctgat tccgacgagc ctcaatgagg cgctacggct cacgcccgaa gcggcacgcg
      901 agtcgagccc gatactttgg ccgccacctc cggccgatag agtattcgtt gcggcagtcg
      961 gcggagctga aagccaggag ttcttgcgtc agagcctgga gatcacggca gcttggtcgg
     1021 cagccggtgt gaaggctgag tgcatcgtca ttccgcatgc caaccatttc acggtggtcg
     1081 acgaactgac gcgcccggaa agcgcgatgg tgtttcgcgt gctgcagttg gctcagcagt
     1141 gagaaccctt gcaggcgggt gacagccttg tgaattcgcg gcctgaaagt tgacgattat
     1201 ttgcgatttc atcagcccac ctcatggcag agtgattgga ttgcatgctg gttcgcggaa
     1261 aggcaccgtg tttgtgggcg cgagcgttga ccatagtcct gatggttatg ctcgctatcg
     1321 cttccccgtt ggcgcacgtg gtgcttccgc aggctcttgc caatcagtcg tttgttcacg
     1381 gtggttccaa gcatgctcat cctcatcacc atggcgatga gctgcccatc gccgctctcg
     1441 gggcattcgg tgaaaacagc tcccacgaag cctgttggcc ggaaagcaat tgcgcaaagc
     1501 tccatgagtt ctgtcccgac tgcagctact tctgccaccc ggttttcgac gtgactggag
     1561 agtatctgat cgaaaaccag ggccggccag tcgcctattc cgcccccaat gccatcctca
     1621 agggggttat tcccgacgta cttcctcgac caccccagat cggctgatcg cgcaagtcga
     1681 agaaacacag tccgggttgc gcattcgtgg tttgcgcaca ttccggacgg ccaaggtcaa
     1741 caaattcagg acacttccga tgaagagcta tcttccatca cttgcgacga tcttcgcgat
     1801 cgcattcgct gcaaccgctg ctcctgctgc ggcgcagtcg gcggactcgc acggaggcca
     1861 tggacatggc gaagcgagca tgcacagcac ggagcatggc cacggtcatg gctcacactc
     1921 cgccttgccc atcaaaggca cggtgaacga ggtctccaag gcctacgccg aggccaacgc
     1981 aaagatgcac gccggaatgg acatcgagtt caccggcgaa gcggacgtgg acttcgtccg
     2041 aggtatgatc ccgcaccatc aaggcgccgt tgatatggcg cgcatcgtcc tgcagtacgg
     2101 caaggacccg gaaatccgga agctcgccgg cgagatcatc aaagcccagg aggcagagat
     2161 ctcgatgatg caggagtggc tcaagaagcg cgggctgcag taacgctgta ttaagcaaga
     2221 acgtctggca ccggctcagt gcgatgccgg tgctggcttt gccgccgctg ccatgtagtt
     2281 gacgtcgata tccgcgccca acgtccagcg accggttaat gggttataga tcagcccctc
     2341 gaatgtgggc tgctgcaacc ctgcgctcgt acaatatcgc gccagctcgt caggcgtgac
     2401 gaggcggtcc cactgatgcg tgccaaccgg aagccaccgc aggatgtatt cggcgccgat
     2461 gatcgccagt gcatatgcct tcagcgtacg gttgatcgtc gacgcgataa agatcccgcc
     2521 cggcctcacc agtgcagcac atgccgcaat gaatgcctgg gggtcgggga catgctcaat
     2581 gacctccagg caaaccaccg cgtcgaactg cctctgctcg tctacaagat cctcgacccg
     2641 gcaggcgcgg tagtcgatgt cgaggccctg gggctccgca tggcgaatgg ctgccgcgat
     2701 gttctccttt gcggggtcga tcccagtaac gcgagccccc agccgggcca gcggctcgga
     2761 gatcaacccg ccgccgcatc ccacatccag aatagagagg ccggagagag atttcgcgga
     2821 gctctccggc aggccgaaat ggtgaacgag gtgcgaacgg atgaaggaaa gccgggccgg
     2881 accgatcttg tgcagcggcc gaaattcacc ttccggatcc caccacttcc ccgccaattg
     2941 ggcgaagcga tcgatttcct gaggatcca
//
LOCUS       DASUMG010000322         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1085865, whole genome shotgun sequence.
ACCESSION   DASUMG010000322 DASUMG010000000
VERSION     DASUMG010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085865"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /gene="otsB"
                     /locus_tag="VFX46_05295"
     CDS             complement(<1..676)
                     /gene="otsB"
                     /locus_tag="VFX46_05295"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEX5600027.1"
                     /translation="MTLPDLDFEKDALFLDLDGTLIDIAPHPDDVVAPPDLIRSLGAL
                     ERRLGGALSVISGRPIAAIDALLAPLRLPASGVHGAEIRFEAGQDKIARAAPPIPNGV
                     RWLLAPLAEIPGVLVEDKTIAIAVHFRQAPDAAERVKEVVEAAVAAHRGDQLTVVPGK
                     CVFEIKHARFSKGTAVEEFMARPPWAGRRPVFVGDDVTDEAAFAVLPKWNGVGIAVGQ
                     PRPGAVA"
     gene            complement(982..>3436)
                     /locus_tag="VFX46_05300"
     CDS             complement(982..>3436)
                     /locus_tag="VFX46_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002968469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEX5600028.1"
                     /translation="IFGPNRNEPLDEEAVRAAFTELAEQIGDGRTPEEVADGFLRIAV
                     ENMANAIKKISVQRGYDVTEYVLNCFGSAGGQHACLIADTLGMETVMIHPLSGLLSAY
                     GMGLAKIRASREQSVEAPLEAASMQEVETLSEKLRGDVIAEVAAEGVLPDEISTTVNL
                     HLRYEGTDTALPIRMSGPEEMRAEFERLHRQRFGFISPEKRVFIAAVEVEAVGGGVSA
                     EERHLDTNRQDPQPTRATRFFSRGAWHEAPVYIREDIAPGHRIMGPALIIEPNQTVVV
                     EPGWRLEVSPLNHLILTRAEPRKREVLGKTADPVLLEVFNNLFMSIAEQMGEALRNTA
                     QSVNIKERLDFSCAVFDSEGQLVANAPHMPVHLGSMDRSVETIVRERKGTMRPGDVYM
                     LNAPYNGGTHLPDITVITPVFGPDGSEILFYVASRGHHEDIGGLTPGSMTPRATHIEE
                     EGVYIDNFKLVDQGRFLEAETYALLTGAKYPARSPHKNIADLKAHVAANARGAAELEK
                     MVAHFGLDVVKAYMRHVQDNAEESVRKLLSRLEDGEFRVEMDQGTHVQVKITVDREKR
                     EATVDFTGTSPEQPNNFNAPAPVTRAATLYVFRVMVDEPIPMNAGCLKPIRLIIPERS
                     MLSPQYPAAVVAGNVETSQIVTNCLFAAMGAMASSQGTMNNLTFGNDKYQYYETICSG
                     SPAGPGFDGTAAVHVHMTNTRLTDPEILELRYPVVLEEFSIRRGSGGRGRWSAGDGTY
                     RRIRFLEQMDCAILSGYRRVRPFGLFGGEPGECGENLVRRNDGRMERLEGCDQTVLAP
                     GEAIIIKTPTGGGYGKPEP"
BASE COUNT          599 a         1069 c         1121 g          647 t
ORIGIN      
        1 ctgcgacagc acctggtcgg ggctgtccaa ccgctatccc cacgccattc catttgggca
       61 atacggcgaa tgctgcctca tccgttacgt cgtctccgac gaataccggc cgccggccag
      121 cccagggtgg acgtgccatg aactcttcga cagcagtgcc cttgctgaag cgtgcgtgct
      181 tgatctcgaa gacgcatttc ccgggcacaa cggtgagctg atcgccgcga tgggcagcga
      241 cggctgcttc caccacctcc tttacgcgct cggcggcgtc gggagcttgg cggaaatgga
      301 cggcgatggc gatcgttttg tcctcgacga ggacgcctgg aatttcagcc aaaggggcca
      361 gcaaccatcg aactccgttt gggatgggtg gcgcagctcg cgcgattttg tcctgtcccg
      421 cctcgaaccg gatttcggct ccgtgaacac cggaagcagg gaggcgtagc ggcgcgagca
      481 gggcgtcgat cgcagcaatg gggcggcccg agatgaccga aagcgctccg cccagccgcc
      541 gctcgagagc gccaagagag cggataagat ctggcggcgc gacgacatcg tcgggatgcg
      601 gagcaatgtc gatcaaggtg ccgtccaagt cgaggaagag ggcatccttt tcgaaatcca
      661 ggtccgggag ggtcatgtaa gctttttgta agaggggtgg gcctggcaaa cgcgcactcc
      721 cacgtcacgg gcgcgaacgc ctcgcgagcg gacgcgtctg cctgagattg tttccggatc
      781 gctgcctttc ggcagcgcga tccgcggagg agataatata gacgccctac aatctcaatg
      841 cgtcgacacg cccagcggga ggccaggcag acaaacagct tctactggca gcgcagatta
      901 ggcggcgcgg ctcctatcga gacgttctcg cgcaacgacg accgggcgat tgcgtgaccc
      961 aaccggcaga gggcgctggg cctagggctc gggcttgccg tagccgccgc cggtgggcgt
     1021 cttgatgatg atcgcctcac caggcgccag cacggtctga tcgcagcctt ccaggcgctc
     1081 cattctgccg tcattccggc ggacgaggtt ctctccgcat tcgcccggct cgccgccgaa
     1141 aagcccgaac ggccgcacgc gtcggtagcc ggagaggatg gcgcagtcca tctgctccag
     1201 gaaccggatg cgccggtagg tgccatcgcc cgcggaccag cgtccgcgcc cgcctgagcc
     1261 gcggcggatg gagaactctt cgagcacgac cgggtagcgc agctcgagaa tttcggggtc
     1321 ggtgaggcgc gtgttggtca tgtggacgtg cacggcggcc gtgccgtcga acccaggtcc
     1381 ggccggagag ccggagcaga tggtctcata gtactgatac ttgtcgttgc cgaaggtcag
     1441 gttgttcatg gtgccctggc tcgatgccat ggcgcccata gcggcgaaca ggcagtttgt
     1501 gacgatctgg ctcgtctcca cgttgcccgc cacgacggcg gccgggtact gcggcgacag
     1561 cattgaccgc tccgggatga tgagccggat gggcttgagg cagcccgcgt tcatcgggat
     1621 cggctcgtcg accatcacgc ggaacacata aagcgtcgcc gcgcgggtca ccggcgccgg
     1681 agcattgaag ttattgggct gctccgggct cgtgccggtg aagtcgactg tggcctcccg
     1741 tttttcgcga tccacggtga tcttcacctg cacgtgcgta ccctggtcca tctcgacccg
     1801 gaactcgccg tcctcgagcc ggctcagaag tttgcggaca ctttcctcgg cattgtcctg
     1861 cacgtggcgc atgtaggcct tcactacgtc gaggccgaag tgggcgacca tcttctcgag
     1921 ctcggcagcg ccgcgagcat tggcggccac gtgcgccttg aggtcggcga tgttcttgtg
     1981 cgggctgcgg gccggatact tagcgcccgt caacagggcg taggtttcgg cctcgaggaa
     2041 gcgaccctga tcgaccagct tgaagttgtc gatgtagacg ccttcttctt cgatgtgcgt
     2101 ggcgcgcggc gtcatcgaac ccggcgttag gccgccgata tcctcgtggt ggccgcggct
     2161 cgcgacatag aacaggattt ccgaaccatc cgggccaaac accggcgtga tgaccgtgat
     2221 gtccggcagg tgcgtgccgc cattgtaggg cgcgttcagc atgtagacgt cgcccgggcg
     2281 catcgtgcct ttgcgctcgc gcacgatggt ttccaccgag cgatccatcg agccgaggtg
     2341 caccggcatg tgcggagcat tggcgacgag ctggccttcc gagtcgaaaa cggcgcagga
     2401 aaagtcgagc cgctccttga tgttcacgga ctgggccgta ttgcgcagcg cctcgcccat
     2461 ctgctccgcg atcgacatga agaggttgtt gaacacctcg agcaaaacgg ggtcggcggt
     2521 cttgccgagc acctcgcgtt tccgcggctc tgcgcgcgtg agaatgaggt gattgagggg
     2581 tgagacctcg agccgccagc cgggctccac cacgactgtc tggttcggct cgatgatgag
     2641 cgcgggcccc atgatgcgat ggccaggagc gatatcctcg cggatataga ctggcgcctc
     2701 atgccacgcg cccctggaaa agaaccgggt tgctctagtc ggttgcggat cctggcggtt
     2761 ggtatcgagg tgccgctctt ctgcgctgac cccgccaccc acggcctcca cctcgacggc
     2821 agcgatgaaa acgcgctttt ccggagagat gaaaccaaat cgctggcgat gcaggcgctc
     2881 aaattctgcc cgcatctctt ccgggcccga cattcggatc ggcagggctg tgtcggtgcc
     2941 ctcgtatcgg agatgcaggt tgacagtcgt cgagatttcg tcaggcaata cgccctcggc
     3001 cgcaacctcg gctatcacgt cgccgcggag cttttcggac aaagtctcca cctcctgcat
     3061 cgacgcggcc tcgagcggcg cctcgacgga ctgctctcgg ctcgcgcgga ttttggccag
     3121 acccatgccg taggccgaga gaagacccga gaggggatgg atcatcaccg tttccatgcc
     3181 gagcgtatcg gcgatgagac aggcgtgttg acctccggcg gatccgaaac agttcagcac
     3241 gtactcggtc acatcgtatc cgcgctgcac ggaaatcttc ttgatggcgt tagccatgtt
     3301 ttcgacggcg atgcgcagga agccgtctgc gacctcctcg ggcgtgcggc catcaccaat
     3361 ctgctcagcg agctcggtga atgcagctcg cacggcttct tcgtcgagcg gctcgttgcg
     3421 attggggccg aagatc
//
LOCUS       DASUMG010000323         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_524485, whole genome shotgun sequence.
ACCESSION   DASUMG010000323 DASUMG010000000
VERSION     DASUMG010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524485"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VFX46_05305"
     CDS             complement(<1..754)
                     /locus_tag="VFX46_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX5600029.1"
                     /translation="MASPADVLRLIKEKDVKFVDLRFTDTKGKLQHVTAEASCIDEGV
                     FADGFAFDGSSIAGWKGIEASDMLLSPDPATAHLDPFFAQTTLAIFCDVIEPSTGELY
                     ERDPRGIAKKAEAYLKQTGIGDTAYFGPEAEFFIFDDVRFTTDPFNTGFKLDSTELPT
                     NSGTEYEMGNLGHRPRTKGGYFPVPPVDSCQDIRSEMLSVMAEMGVKVEKHHHEVAAA
                     QHELGIKFGPMVTIADHLQIYKYVAHNVAQAYG"
     gene            complement(848..1186)
                     /locus_tag="VFX46_05310"
     CDS             complement(848..1186)
                     /locus_tag="VFX46_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX5600030.1"
                     /translation="MKKIEAIIKPFKLDEVKEALQEIGLQGITVLEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVIPDEMLDKAVEAIQRAAKTGRIGDGKIFISSIEEAIRI
                     RTGETGTDAI"
     gene            1524..1904
                     /locus_tag="VFX46_05315"
     CDS             1524..1904
                     /locus_tag="VFX46_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007799592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5600031.1"
                     /translation="MRVTGFDHLVLTVASIERTVEFYERVLGMTREVFGPERRTALRF
                     GPHKINLHEQGKEFEPKAAKPTPGSADLCFLVEGFEGIADHLAAAGVPIIEGPVERTG
                     AQGRIISYYIRDPDMNLIELSHYP"
     gene            1919..>2151
                     /locus_tag="VFX46_05320"
     CDS             1919..>2151
                     /locus_tag="VFX46_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX5600032.1"
                     /translation="MTTTGVDGLELLTSAEMGRADELTIAGGTPGLTLMENAGQGVAE
                     EVEWIAEPGARVAVLCGPGNNGGDGFVAARLLR"
BASE COUNT          377 a          614 c          679 g          481 t
ORIGIN      
        1 tgccgtaggc ctgggcgacg ttgtgggcca cgtacttata aatctgcagg tggtccgcga
       61 tggtgaccat cgggccgaac ttgatgccga gctcgtgctg ggcggccgcg acctcgtggt
      121 gatgcttttc gaccttgacc cccatctcgg ccatcacgga tagcatctcg gagcggatgt
      181 cctggcagct gtcgaccggc gggacgggga agtagccacc cttcgtccgc ggccggtgac
      241 ccaggttacc catctcatat tcggtgccgg agttcgtcgg cagttccgtc gagtcgagct
      301 tgaagccggt gttgaacgga tcggtcgtga agcgcacgtc atcgaagatg aagaactccg
      361 cctccggacc gaagtaagcg gtgtcgccga tgccagtctg cttcagatag gcttcggcct
      421 tctttgcgat gccgcgcgga tcgcgctcgt acagctcacc ggtcgaaggc tcgatgacgt
      481 cgcagaagat cgccagcgtc gtctgggcga agaacgggtc gaggtgtgcc gtagccggat
      541 cggggctcag cagcatgtcg gaagcctcga tgcccttcca tcccgcaatg gatgaaccgt
      601 cgaaggcgaa gccgtccgca aaaacgccct cgtcaatgca gctcgcctct gcagtcacgt
      661 gctgcagctt gcccttggta tctgtgaacc gcaggtctac gaatttcacg tccttttcct
      721 tgatgagcct aaggacgtcg gctggacttg ccattctaca actcccctct ggtgtaggct
      781 tggttgctag gttgggtgct tggatcagtg ggtatgagcc gcagctctgt ccggttttgc
      841 tcgatgatca aatggcgtcc gttcccgtct ctccggtgcg gattcggatc gcttcctcga
      901 tcgacgatat gaagatcttg ccgtcgccga tgcgtccggt cttcgccgcc ctttgaatgg
      961 cctctaccgc cttgtcgagc atctcgtcag gaatcacgac ctcgattttc actttgggca
     1021 gaaagtcgac gacgtactcg gcgccccgat agagctcggt atgacccttc tgacgtccga
     1081 agcctttggc ctcgaggacc gttatccctt gcaagccgat ttcttggaga gcctctttca
     1141 cctcatcgag cttgaagggt ttgatgatcg cttcgatttt tttcatcgtg tctcccggac
     1201 gactgcttgt tggctcgccc ctcgctaagc agaaggcgtg ccagatggtt actgcggggc
     1261 ggactgcccg aaaattcaat gcgttggcct tattctcgtt catctgcaga ctgccattcg
     1321 atagccattc ttactaagaa ttaggcgcat tggttatttt ttatgcaggc agtttgcttg
     1381 ccgctgcctc tctgttgggc aactgtggcc gcgaattgaa ccggtaaagg ctggagcgga
     1441 tgggttgact gctggaatcg cctgcgcgtt catggcgagc ggcgacgtcg tgggtacgac
     1501 cggtgtcaac agggaggcat tcaatgcgcg tcacaggctt tgaccatctc gttctcaccg
     1561 tcgcttccat cgaacgaacg gttgagttct acgagcgcgt gctcggaatg acgcgcgagg
     1621 tgttcggtcc cgagcggcgg actgcgctgc gctttgggcc gcacaagatc aacttgcatg
     1681 agcaggggaa ggagttcgag ccaaaagcag ccaagccgac cccaggctcg gcggatctgt
     1741 gcttccttgt ggagggtttc gagggcatcg cggatcacct cgcagcggcc ggtgtgccga
     1801 taatcgaggg gccggtggag cgaaccggtg cgcaagggcg gatcatctcc tattacatcc
     1861 gcgatccgga catgaacctg atagagctca gccactatcc ctgaggggag ctgaggcgat
     1921 gaccaccacc ggcgttgatg ggctagagct tctgacctcg gccgagatgg ggcgagcgga
     1981 cgagctgacc atcgcgggcg gtacgccggg gctcaccctg atggagaacg ctggccaggg
     2041 cgtcgctgaa gaggtggaat ggatcgcgga gcctggcgcg cgggtcgctg tgctctgcgg
     2101 gccgggaaac aatggcgggg acggcttcgt cgccgctcgc ctgctccggg a
//
LOCUS       DASUMG010000324         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_926022, whole genome shotgun sequence.
ACCESSION   DASUMG010000324 DASUMG010000000
VERSION     DASUMG010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926022"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VFX46_05325"
     CDS             <1..829
                     /locus_tag="VFX46_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5600033.1"
                     /translation="PARAIDRMISAAVDLETFIRSEIGRQGKCDLHVFGGPTVSIDAR
                     ALAVLSSILGDIVASTARIDPQAAIAFDVVWSCDHQGQCVVELSLPSSCGARGLSGTG
                     AIRSKQRLELEQSGAGMIRCEMSDSGARIIIPARYIASRRTLEDWSKGQSVDPLKDRS
                     VLVVEDQLLIALDLETLLLEHGAASVRLCGTVEDALQSIRMERPDIAILDVNLGSTTS
                     FPVAFELQRLGVPLIFATGYGNEVDVPPALRSIPLVAKPYCLETIREGLRSTFAVHA"
     gene            complement(891..1625)
                     /locus_tag="VFX46_05330"
     CDS             complement(891..1625)
                     /locus_tag="VFX46_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003586003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEX5600034.1"
                     /translation="MAESGRRYFARSVGALSLAGLLALGLSTISPVDFSRAAAPDRHT
                     ESVEGRALVIDGDTIVIEGTRVRLEGIDAPESGQQCLRLDGQSWRCGIAAAQALAELV
                     SDKHVRCEGSERDRYDRLIGVCRVGSLEINAEMVRRGLAWAFVRYSKRLLNEEREARS
                     RRLGIWQAENEPAWDYRARRWAQSQAQAPGGCAIKGNISRQGRIYHMPGSAYYDAVEI
                     NPSRGERWFCTENEAIAAGWRPAWTN"
     gene            1855..2199
                     /locus_tag="VFX46_05335"
                     /pseudo
     CDS             1855..2199
                     /locus_tag="VFX46_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452917.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
BASE COUNT          434 a          673 c          697 g          472 t
ORIGIN      
        1 tccagccagg gcgatcgatc gcatgatttc cgccgcagta gatctggaga cgttcatccg
       61 ctccgaaatc ggccggcagg gtaagtgcga cttgcacgtc ttcggcggtc ccaccgtgtc
      121 gattgatgct cgcgctctcg cggtgctgtc gtccatactc ggcgatatcg ttgcatcgac
      181 ggcaagaatt gacccgcagg cggctatcgc cttcgatgtc gtctggtcct gtgatcacca
      241 gggccaatgc gtggtggagt tgagcctccc ctcgagttgc ggcgcacgcg gattgtcagg
      301 cactggcgct atccgctcga agcagcggct tgagctggag cagtctggtg ccgggatgat
      361 ccgttgcgaa atgtcggata gtggcgcacg catcattatt ccggcccgct atatcgcaag
      421 ccggcgcacg ttggaggatt ggtcaaaagg acaatcggtc gacccgctaa aagatcgatc
      481 agttcttgtt gtcgaggacc aactcctgat cgcgctggat ctggaaacgc tcctccttga
      541 gcatggagcc gcgtcggttc gtctttgcgg gacggtcgag gatgcgctgc agtcgatcag
      601 aatggagcgg ccagatatag ctattctcga tgtcaattta gggagcacaa cgtcttttcc
      661 agttgcgttc gaactccagc ggcttggtgt tcctctgatt tttgcgactg gatatggtaa
      721 cgaggtggat gttccaccgg cgctgcgctc aattcccctc gttgcgaaac cctattgcct
      781 ggaaacgata cgcgaaggat tacgctccac cttcgcggtg catgcgtaaa caagccggtc
      841 cggcgatttt ccaaccactt aatcaagcaa ttccggcatt accattggtt tcaattcgtc
      901 cacgccggcc gccagccagc cgcaatcgct tcgttttccg tgcagaacca acgctccccg
      961 cgtgatggat tgatttcgac ggcgtcgtag tatgcgcttc cgggcatgtg atagatccgc
     1021 ccttgacgcg aaatattgcc ttttattgca caaccgcccg gtgcttgcgc ttgagactga
     1081 gcccagcgcc tggcacggta atcccaagcg ggctcattct cggcctgcca aattcctagg
     1141 cgtctactgc gagcctcgcg ctcttcattc aacagccgtt tcgaataacg gacgaatgcc
     1201 caggcaaggc cgcgccgcac catttccgca ttgatttcca gagaccccac gcggcaaacg
     1261 ccaatcagcc gatcatatcg atcgcgctct gagccctcgc accggacgtg cttatcggaa
     1321 accaactcgg cgagagcctg cgctgcagct atcccgcatc tccaggattg cccatcaagg
     1381 cgaaggcact gctggcctga ctcaggcgca tctattcctt caaggcgtac gcgtgttccc
     1441 tcaattacga tggtgtcgcc atcgatgacg agcgcccggc cttccacgct ttcagtgtga
     1501 cgatccggag cagctgcccg cgagaaatcg accggagaga tggttgaaag cccgagcgcg
     1561 agcagaccgg ccagcgaaag cgctcccacg ctgcgggcga agtatcggcg tccagattca
     1621 gccataagtt gaagctgatc tgattcgtgc ggcagcgtcg aggcgccgaa gtgcccaaac
     1681 ttgtcagaaa cttaacgtgc tcaacgattg ggctgtctta agtgtgactg ggcaaatctc
     1741 gcacgcggtg agcggcagcc aggtcgcgca gcgcttcagt gacaccttgc cactcagctc
     1801 ccgacagggg catgttcggt tacaagaacc gtttgcaacc ggcgggagca gaaaatgtcg
     1861 gatccgggac aggttcattc cgctgcggct cgtgtcggcg tgctgcgcgc ggcgcgtgat
     1921 tggctagagc gagacgggcg ggtcgcactg gcgaccgtga tcgacacctg gggctcggcg
     1981 cccgtgccgg tcggaggaca gctcgccata gccgcggatg ggagcttcga aggctcggtt
     2041 tcgggcggct gcatcgaagg cgaggtgatc acggaggctg aggaggttct gaacgacggc
     2101 cgcccgaggg cgctggagtt tggcgtcgcc gacgagacgg catggcgcgt cggcctaccc
     2161 tgcggcgggc aggtgagggt cttcgttgaa cggctggatc gctcaggagg cggtctcgag
     2221 tacctggaga aggctctcgc tgccgacggc cgacgcgaag gcatcctggt gcgcac
//
LOCUS       DASUMG010000325         2056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_364003, whole genome shotgun sequence.
ACCESSION   DASUMG010000325 DASUMG010000000
VERSION     DASUMG010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364003"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VFX46_05340"
     CDS             complement(<1..345)
                     /locus_tag="VFX46_05340"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600035.1"
                     /translation="MDTGLGPLGILAGNGDLPLEVANAVRAQGRDVHLVAIRGEAEPG
                     IEAHPHTWIGLGQIGGMLRAFREAGCRDLVILGGVRRPDLLKLRPDLGFFRNLPLILS
                     LMVGGDDSVLRRV"
     gene            complement(348..1151)
                     /gene="lpxA"
                     /locus_tag="VFX46_05345"
     CDS             complement(348..1151)
                     /gene="lpxA"
                     /locus_tag="VFX46_05345"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX5600036.1"
                     /translation="MADAQIHPTAIVESGARLGAGVKIGPYCLVGPEVTLGDGCELIS
                     HVVVAGHTTIGPRTRIFPFASIGHQPQDLKYKGESSKLTIGSDCLIREGVTMNPGTAG
                     GGLETVVGNNCAFLANSHVGHDCRVGNNVVFSNNVMLAGHCTVGDYAILGGGAAVIQF
                     ARIGAHAFLGGMSGLENDLIPYGMAVGNRAHLSGLNIIGLQRRGFSRETIHDLRRAYR
                     LLFAAEGTLKERLDDVAAEFESHPLVQEIVAFIREGGKRSLCTPRHSEG"
     gene            complement(1144..1644)
                     /gene="fabZ"
                     /locus_tag="VFX46_05350"
     CDS             complement(1144..1644)
                     /gene="fabZ"
                     /locus_tag="VFX46_05350"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX5600037.1"
                     /translation="MNDASAKGQLGTADIARLLNLLPHRYPFLLVDRIFDMDRDESAV
                     GLKNVTINEPFFQGHFPSYPVMPGVLIIEGLAQTAGALCVHSLGESYRPQLVYFMGID
                     KAKFRKPVVPGDQIHFHVRKIRSRGRAWRFYGEARVNGQVAAEAEISAMIVDTPETQA
                     IASQNG"
     gene            complement(1701..>2056)
                     /locus_tag="VFX46_05355"
     CDS             complement(1701..>2056)
                     /locus_tag="VFX46_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX5600038.1"
                     /translation="TIIGEGTKIDNLVQIGHNVVIGRHCVIVAHCGISGSTELGDFVV
                     MGGQSGTVGHIKIGTGAQVAGASHPKDDVPAGARVGGTPARPLKDWARELAVLSRLGK
                     QGRLAPGTDDGEGGT"
BASE COUNT          373 a          689 c          632 g          362 t
ORIGIN      
        1 gacccggcgg aggacgctgt cgtcaccgcc gaccatcagg ctcaaaataa gcggcagatt
       61 acggaaaaac ccgaggtcgg ggcgcagctt caacagatcc ggccgccgca caccgccgag
      121 aatgacgaga tcccggcagc cggcctcgcg gaacgcgcgc agcatgccgc caatctggcc
      181 cagaccgatc catgtatgag gatgcgcttc gatgccgggt tcagcttcgc ccctgatggc
      241 gacgagatgc acatcccggc cctgagcgcg aaccgcattg gcgacctcaa gcggcaggtc
      301 gccgttgccg gccaggattc ccagcgggcc aaggcccgtg tccacgttca gccctcggaa
      361 tggcgcggag tgcacaagga tctcttaccg ccctcgcgga tgaaggccac gatctcctgt
      421 accagagggt ggctttcgaa ctcggcggcc acgtcgtcga ggcgctcctt cagggtgccc
      481 tcagccgcga acagcaaccg gtaggcccgg cggagatcgt ggattgtctc ccgcgaaaat
      541 ccccgccgct ggagcccgat gatgttgagc cctgagagat gggcgcgatt gccgaccgcc
      601 atgccgtacg ggatcaggtc gttctcgagt cccgacatcc ctccaaggaa agcgtgagcg
      661 ccaatccgcg caaactgaat gaccgccgcc ccgccgccga ggatggcgta gtccccgacc
      721 gtgcagtgcc ccgccagcat gacgttgttg gagaacacga cgttgttgcc gacgcggcaa
      781 tcgtggccga catgcgagtt ggcaagaaat gcgcagttgt tcccgacgac cgtttcgagg
      841 cccccacccg ctgtcccagg gttcatcgtg acgccctcgc ggatgaggca gtccgagccg
      901 atcgtcagct tcgatgactc gcccttgtac ttcaggtcct ggggctgatg gccgatcgag
      961 gcgaagggaa agatgcgcgt gcgcgggccg atcgtcgtat ggccggccac gacgacatgg
     1021 ctgatcagct cgcagccatc accaagcgtc acctctggcc cgaccaggca gtaagggccg
     1081 atcttcacgc cggctccgag cctggctccg gattcgacga tggccgtggg atggatttga
     1141 gcgtcagcca ttctggctcg caatagcctg agtctcgggc gtatcgacga tcatggcgct
     1201 gatctcggcc tcggccgcga cctgcccatt gacccgtgcc tcaccgtaga agcgccatgc
     1261 gcgaccgcgg ctacgaatct tgcgcacgtg gaagtgaatt tggtcgcctg gcacgacggg
     1321 cttgcggaac ttcgccttat caatgcccat gaaatagacg agctgcgggc ggtagctttc
     1381 accgaggctg tgcacgcaca aggcaccggc tgtctgcgcc agaccctcga tgatgagcac
     1441 gccgggcatg acggggtagc tcgggaagtg cccctggaag aacggctcgt tgatggtgac
     1501 gttcttcagc ccgacggcgg actcgtcacg atccatatcg aagatgcggt cgaccaggag
     1561 gaagggatac ctgtgcggca gcagattgag caggcgcgca atgtcggccg tgccgagctg
     1621 ccccttcgct gacgcgtcat tcatagcctt tccactcctt ctggcacgcg tgtgtgcacc
     1681 tctgctaccc cttccattcc ctatgtcccg ccttcaccgt catcagttcc aggcgcgagg
     1741 cggccctgct tgcctagccg gctcagcacc gcgagctccc gagcccagtc cttgagtggc
     1801 cgcgcgggcg tgccgccgac acgggcaccg gccggcacgt catccttcgg gtgcgaggca
     1861 cccgcgacct gcgcgcctgt gccaatcttg atgtgcccca cagttcccga ctggcccccc
     1921 atcacgacga agtcaccgag ctccgtcgag cccgagattc cgcagtgcgc cacgatcaca
     1981 caatgcctgc cgatgacgac gttgtgcccg atttgcacga gattatcaat tttcgtgccc
     2041 tcgccgatga tcgtgt
//
LOCUS       DASUMG010000326         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_42703, whole genome shotgun sequence.
ACCESSION   DASUMG010000326 DASUMG010000000
VERSION     DASUMG010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42703"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..599)
                     /locus_tag="VFX46_05360"
     CDS             complement(69..599)
                     /locus_tag="VFX46_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEX5600039.1"
                     /translation="MMPATLPTPERDSAYRWGASVLVAAIAIILTALGFEYIGGYRPC
                     PLCLQQRYAYYAGIPVLFGALVLVTAGHRRWAGLLFAIVAAGFLVNAGLGVYHAGVEW
                     KLWAGPDTCQLYLDSAPAGSGGVLEQLKTVRVIRCDEAPWRFLGLSFAGWNALLSIFL
                     ALGAFMAARLSWRRGI"
     gene            complement(601..1182)
                     /locus_tag="VFX46_05365"
     CDS             complement(601..1182)
                     /locus_tag="VFX46_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HEX5600040.1"
                     /translation="MLRRLYDWTMDLAGHKRAPQALFWVAFIESSFFPIPPDVMLMPM
                     VLARRLKAWTYASIATLGSVLGGVAGYLIGLLLFDVLGRPLVELYGYGEKFAEFASSY
                     NEYGALIVFFFGVTPFPYKVITIASGVTQLNIVVFVLASIAARGLRFFLVSGLLYWFG
                     PPIRDFIEQRLGLVTTVFLVLLIGGFVGLRYLM"
     gene            complement(1338..2492)
                     /gene="hemW"
                     /locus_tag="VFX46_05370"
     CDS             complement(1338..2492)
                     /gene="hemW"
                     /locus_tag="VFX46_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX5600041.1"
                     /translation="MGFGVYIHWPFCASKCPYCDFNSHVRIGGIDEARFLAAFRRELR
                     TVRERIGPRRVTSIFIGGGTPSLMQPSTVAGILDEIARLWEIEPGAEVTLEANPSSVE
                     AGRFRGYRTAGVNRVSLGVQSLRDSDLKQLGRLHSVAEAKAAIAVASSTFERFSFDLI
                     YARPGQTAEAWREELEEALAMAGGHLSLYQLTIEDGTPFAALYAAGKLRVPDGEHARA
                     LYELTNDVTAANGLPAYEISNHAVPGEESQHNLLYWRYGEYAGIGPGAHGRLIIGRER
                     WATMNERVPERWLSQVEETGEGWIEAEPLSPREQADEMLLMGLRIDEGLDLDRLERIG
                     GAQVDFALISQLVDQGLLATSAKGRRIKATMAGRMVLNEVVLRLSAGINP"
     gene            complement(2497..>2618)
                     /locus_tag="VFX46_05375"
     CDS             complement(2497..>2618)
                     /locus_tag="VFX46_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX5600042.1"
                     /translation="PDGETLTFGEMDPADKHAISHRARAFALFVEACLEGKQS"
BASE COUNT          566 a          838 c          776 g          438 t
ORIGIN      
        1 tttttcactg attatttgtt cagctttgat gggcgcgtga tgagcagcgg gagcggccaa
       61 ggtcgccgtc aaatgccccg gcgccagctg aggcgagcag ccataaatgc gccgagcgcg
      121 aggaagatcg atagcagggc attccatccc gcgaaggaca gtcctaggaa gcgccaaggc
      181 gcctcatcgc agcgaatgac gcggacagtc ttgagttgct ccaggacacc gccagagcct
      241 gccggcgcgg aatcgagata aagctggcag gtgtcggggc cggcccaaag cttccattcg
      301 acaccggcgt gatagacgcc aaggccggca ttgacgagga agccggcggc gacaatcgca
      361 aacagaagcc cagcccatcg ccgatgcccg gcagtcacca acaccagggc accgaacagc
      421 acaggaatgc ctgcgtagta agcataacgc tgctgaaggc agagcgggca gggccggtag
      481 ccaccgatat actcaaaccc cagcgcagtc agaatgatgg cgatagccgc gacgaggacc
      541 gaggcccccc atcgataggc tgaatctcgc tcaggtgtgg gcagcgtcgc gggcatcatc
      601 tcacatgaga tagcggaggc cgacaaaacc cccgataagc aataccaaaa ataccgtcgt
      661 cactagcccc agccgctgct cgataaagtc gcggatcggc ggcccaaacc aatagagcaa
      721 accactaacg agaaaaaacc tcagtccgcg ggctgcgatg ctggcgagaa cgaaaacgac
      781 gatattgagc tgggtcacgc cactggcgat cgtaatgacc ttgtacggaa acggcgtcac
      841 cccgaaaaag aacacgatca aggcaccgta ttcattgtag gagctcgcaa actccgcgaa
      901 tttctcgcca taaccgtaaa gctcgacgag tggccttccg aggacatcga acaagagaag
      961 gccgatcaga taaccggcca cgccacccaa aaccgaaccg agcgtcgcga tgctggcata
     1021 ggtccatgcc ttgagccggc gggccagcac catcggcatc agcatcacgt cgggtgggat
     1081 cggaaagaac gagctttcga tgaacgcgac ccagaatagc gcctgcggag cccgtttatg
     1141 gcccgccaag tccatcgtcc agtcgtacag tcgtcgtagc atgaccgccg tgcatacgcc
     1201 gccaaccgat ccgtgtccat tggactcgac atcggggccg gagcaagggg ctggacggaa
     1261 agcggcctct tctcccaagc tgctgatcga ttgagttgaa gggtgctttg cggtgggttt
     1321 gcgctggcag cctggtgtca ggggttgatg cccgccgaaa gccgcaacac cacctcgttc
     1381 agcaccatgc ggcccgccat cgtcgccttg atccgccggc cttttgccga cgtagccagc
     1441 aaaccttgat cgacaagctg ggagatgaga gcaaaatcca cctgggcgcc gccgattcgt
     1501 tccaaccggt cgagatccag cccttcgtcg atccgcaggc ccatgagcag catttcatct
     1561 gcctgctcgc ggggactcag tggctctgcc tcgatccagc cctcacccgt ctcttccacc
     1621 tgcgataacc agcgctccgg cacacgttca ttcatcgtcg cccaccgctc gcggccaatg
     1681 atcaggcgac cgtgtgcacc cggaccgatc ccggcgtact cgccgtagcg ccaatagagc
     1741 aggttatgct ggctttcctc accaggcacc gcatggttgg agatttcgta ggcaggcaag
     1801 ccgtttgccg cggtgacatc gttcgtcagc tcgtacagcg cgcgcgcatg ctcgccatct
     1861 ggcactcgca gcttgccggc ggcatagaga gcagcgaagg gcgtcccgtc ctcgatggtc
     1921 agctggtaca gcgacaggtg cccacctgcc attgcaaggg cctcttccag ctcctctcgc
     1981 caagcctctg cggtctggcc cgggcgcgcg tagatcaggt cgaagctgaa ccgctcgaac
     2041 gtgctgcttg cgactgcaat agctgccttt gcttccgcga ccgaatgcag gcgcccaagc
     2101 tgcttcaagt cgctgtctcg tagggactga acgccaagcg agacgcggtt gacacccgcg
     2161 gtgcgatagc cgcgaaaccg gccagcctcg acgctcgacg gattggcctc aagcgtcacc
     2221 tctgcgcctg gctcgatctc ccatagcctg gcaatctcat cgaggattcc ggcgacggtg
     2281 gagggctgca tcagcgaggg cgtgccgcca ccgataaaga tgctcgtcac gcgccgcggg
     2341 ccgatgcgtt cccggacggt acgcagctca cgccggaaag cagcgaggaa gcgcgcctca
     2401 tcgatccccc cgatccgcac gtggctgttg aagtcgcagt acgggcactt cgacgcgcag
     2461 aacggccagt ggatgtaaac cccgaagccc acctcgtcag gattgcttgc cttcgaggca
     2521 ggcttccacg aagagggcga aggcccgggc gcggtgactg atggcatgct tgtctgccgg
     2581 atccatttcg ccgaacgtca atgtctcgcc gtccggca
//
LOCUS       DASUMG010000327         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_926282, whole genome shotgun sequence.
ACCESSION   DASUMG010000327 DASUMG010000000
VERSION     DASUMG010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926282"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VFX46_05380"
     CDS             <1..539
                     /locus_tag="VFX46_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600043.1"
                     /translation="LKPIPGLDLEPIGKEQTLVGMLRDGELDALFTARGPSSFLRGEP
                     HIKRLFPSTREAEQAYYKKTRMFPLMHLVGIRKELVQKYPWLPSSVYKAFCEAKALAM
                     IDLLDVNALMVTLPWLIPETQETMAVMGKDFWAYGIQENMREITALTQYAHEQGLIDR
                     KVAVEELFHPSMFEISKV"
     gene            670..1902
                     /locus_tag="VFX46_05385"
     CDS             670..1902
                     /locus_tag="VFX46_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5600044.1"
                     /translation="MQHDDRVIVVGAGPVGAVTALALVKKGIPVTLLEGEAEPPEDQR
                     AATIHPPTIEMLADLGLTNEAFADDASGGLVSPLFHFRDRVTGELVAVFDINLLKGEV
                     PYPFVVQWEQYKLVRAARPHIEASGIAKVRFSTKMTGLEQRGDHVDVTVINEGGEAEI
                     LRGRYVIGADGGRSTVRRLAGIEFEGFTWPERFIKIGTSFDFGSTGQGFCTRNYFSDP
                     SEWLNLFKVKGNGPPGIWRGIMPVPPDESDEQALSMEGIQRRLRGIHAKEGDFEIPYY
                     ALYSVHQRVAATFNRGRVLLAGDSAHVNNPIGGMGMNGGIHDAMNLAEKLADVWFGRA
                     EPEVLDRYTRQRRKAQVDYVQAQTIQNKKALEEKDPVIRRQHLDDLRRISEDVKLHKQ
                     FLYRSALFDSLNSANAVE"
     gene            1982..>2440
                     /locus_tag="VFX46_05390"
     CDS             1982..>2440
                     /locus_tag="VFX46_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600045.1"
                     /translation="MDVVVDEVSQIWPGLGGTSVVALDRVSHRFRSRRITCLLGPSGC
                     GKSTLIQIIAGLERATGGTVRIEDPQRHDIQHPLGADSVMVWQNLNLFPWRSVIDNVA
                     FGLEMRGMAKAERYERARALIASVGLRGFEQHLPGQLSGGMRQRVALARAL"
BASE COUNT          433 a          814 c          781 g          412 t
ORIGIN      
        1 cgctcaagcc gatccccggg ctcgacctcg agccgatcgg caaggagcag accttggtcg
       61 gcatgctgcg tgacggcgag ctcgacgcat tgttcacggc gcgcggaccg tcatcgttcc
      121 tgcgcggcga accccatatc aagcgcctgt ttcccagcac gcgcgaagcg gagcaggcct
      181 actacaagaa gacccgcatg tttccgctga tgcacctcgt cggcatccgc aaggagctgg
      241 tgcagaaata tccctggctc ccgtccagcg tctacaaggc gttctgcgaa gccaaggcgc
      301 ttgccatgat cgacctgctc gacgtcaacg cactgatggt gacgctgccc tggctcattc
      361 cggaaaccca ggagaccatg gcggtcatgg gcaaggactt ctgggcctac ggcattcaag
      421 agaacatgcg cgagatcacg gcgctgaccc agtacgccca tgagcaggga ttgatcgacc
      481 gcaaggtggc ggtcgaggaa ctgtttcacc cgtcgatgtt cgagatctcc aaagtctgat
      541 cacgggctgg ctgtttctta ggcgtgtccc gagcgccgcg ccgccgggac gcaagcctgc
      601 aatgccgcac ggctcacttg tccgccgcgc acgagttggt gtgtatagtg cggaagttcg
      661 gagttggtca tgcagcatga tgatcgggtg attgtggtcg gcgccggccc cgtcggggcg
      721 gtgacggcgc tcgcattggt caagaagggc attccggtca ccctgctgga gggcgaggcg
      781 gagccgccgg aggatcagcg cgccgccacg atccatcctc ccaccatcga gatgctggcc
      841 gacctcggcc tcacgaacga agccttcgcc gacgacgcca gcggcggact ggtctcgccc
      901 ctcttccatt ttcgcgaccg cgtcaccggc gagctcgtgg ccgtgttcga catcaacctg
      961 ctcaagggcg aggtgcccta cccgttcgtg gtgcaatggg agcagtacaa gctggtgcgg
     1021 gcggcccgac cgcacatcga agcgagcggc atcgccaagg tccgcttctc caccaagatg
     1081 acgggcctcg agcagcgcgg cgatcatgtc gacgtcaccg tgatcaacga gggcggcgag
     1141 gccgagatcc tgcgcggccg ctatgtcatc ggcgccgacg gcggacgcag caccgtgcgg
     1201 cggctggcgg gaatcgagtt cgagggcttc acctggcccg agcgcttcat caagatcggc
     1261 accagcttcg atttcggctc gaccggccag ggcttctgca cccgcaacta cttctccgac
     1321 cccagcgagt ggctcaacct gttcaaggtc aagggtaacg gcccccccgg catctggcgc
     1381 gggatcatgc cagttccgcc cgacgagtcc gacgagcagg ccctgagcat ggaaggcatc
     1441 cagcgccgcc tgcgcggcat ccatgccaag gagggcgact tcgagatccc ctactacgcc
     1501 ctctattcgg tgcatcagcg cgtcgccgcc accttcaaca ggggccgcgt gctgctcgcc
     1561 ggcgacagcg cccatgtcaa caacccaatc ggcggcatgg gcatgaacgg cggcatccat
     1621 gacgccatga acctggcgga gaagctcgcc gacgtctggt tcggccgcgc cgagcccgag
     1681 gtgctcgacc gctacacccg ccagcgccgc aaggcgcagg ttgactacgt gcaggcgcag
     1741 accatccaga acaagaaagc gctggaggaa aaagatcccg tcatccgccg ccagcatctc
     1801 gacgatctca ggcgcatctc cgaggacgtg aagctgcaca agcagttcct gtaccggtcc
     1861 gccctgttcg acagtctcaa ttcggcgaac gcggtggagt gagccggcgc atggcgagcg
     1921 gcgggtagcg acagacatcg gttcgctact ccccctgcgc cattcgtggc atgctcacag
     1981 catggacgtg gtcgtcgacg aggtgtcgca gatctggccg gggcttggcg gcaccagcgt
     2041 ggtcgcgctc gatcgtgtct cccatcgttt ccgctcccgg cgcatcacct gcctgctcgg
     2101 accatcgggg tgcggcaaga gcaccctgat ccagatcatc gccgggctcg aacgcgccac
     2161 cggtggcacg gttcggatcg aagacccgca gcgccacgat atccagcatc cgctcggtgc
     2221 cgactcggtg atggtgtggc agaacctcaa cctttttccg tggcgcagcg tcatcgacaa
     2281 tgtcgccttc ggcctggaaa tgcggggcat ggccaaggcc gagcgttatg agcgtgcccg
     2341 cgcgctcatc gcctcggtcg ggttgcgcgg ctttgagcag cacctgcccg ggcagttgtc
     2401 gggcggcatg cgccagcgcg tcgcgttggc gcgcgcgctc
//
LOCUS       DASUMG010000328         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_443768, whole genome shotgun sequence.
ACCESSION   DASUMG010000328 DASUMG010000000
VERSION     DASUMG010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443768"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFX46_05395"
     CDS             complement(<1..404)
                     /locus_tag="VFX46_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007768811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX5600046.1"
                     /translation="MDPTKLGDELATFSEAVIAWALSLLPNAIVALLILAIGWAVAGW
                     AERAVGRLLDRGRIEPTLHGVTTAVVRYGILVLVVVAALGQVGVQTTSILAVLGAIGL
                     AIGLALQGTLANIAAGLMLLWLRPFRVGDFIEA"
     gene            complement(517..1701)
                     /locus_tag="VFX46_05400"
     CDS             complement(517..1701)
                     /locus_tag="VFX46_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5600047.1"
                     /translation="MTASGNKHEASAGPQGARFRTRGRGNFLLGLVVLGAMALRGRRA
                     PSADHQGAAYDGPPTEGSLGQTKSLSDMNAPANSADATTRGQDAPTPTHIPARGWWDI
                     AKRVYQRIQEHRVMLVAAGVSYYALLALFPALAALVSLFGLVADPASIQQQLSNLQGV
                     VPEEALQIIGDQISRIQAQGGRTLSLYFFIGLAVSLWSANAGVKSVFDALNIVYEEPE
                     RRSFLQYNLHALLFTLGGILFIILAVSGIVILPAMFAFVGLESMSGRIISLLRWPLLL
                     VALLLALALLYRYGPCREKPQWKWVTPGSIFTAVVWLAGSMLFSWYVSSFGTYNETYG
                     SLGAVIAFMVWMWLSATIVLVGAEINAQSEFQTRADSTTGEPRSMGERGANVADNVAN
                     SL"
     gene            1952..>2361
                     /gene="rpsB"
                     /locus_tag="VFX46_05405"
     CDS             1952..>2361
                     /gene="rpsB"
                     /locus_tag="VFX46_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019836567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5600048.1"
                     /translation="MRQLLEAGVHFGHQAHRWNPKMAEYIFGQRNKIHIVNLERTLEK
                     YNDAMKYVRQLAARKGTILFVGTKRQARDIIAEEARRASMPFVDQRWLGGMLTNFKTV
                     KQSVKRLKDLETMAEDGTFDRMSKREALSHKRELT"
BASE COUNT          487 a          736 c          744 g          394 t
ORIGIN      
        1 gcctcgatga agtcgccgac gcgaaagggt ctcagccaga gcagcatgag tcccgctgcg
       61 atgttggcga gtgtgccctg caacgcgagg ccgatggcga ggccaatggc gccgagcacc
      121 gccagaatgc tcgtcgtctg aacgcccacc tggcccagcg ctgccacgac gacgaggacc
      181 aaaatgccgt atcgtacgac ggcagtggtc acgccatgca gcgtcggttc gatgcggcct
      241 cggtcaagca atcgtccgac ggcccgttcc gcccagcccg ccacggccca gccgatcgcg
      301 aggatcagca aggcgacaat tgcattcggc aacagggaaa gcgcccacgc gatcacggct
      361 tcagagaacg tggcgagttc atccccgagc tttgtcggat ccatcttgaa atcctcccgc
      421 aggaatcgtc agtgaccgaa ctctttcgga ggataacgcg ggagcgtgca gaggaggtcg
      481 aaaagagcgg tggcggaccg atttgcggat ggggcctcaa aggctgtttg ccacgttatc
      541 cgcaacgttc gcgccgcgct cgcccattga tcgcggctcg ccggtcgtgc tgtccgcgcg
      601 cgtctgaaac tcggattgcg cgttgatctc cgcgccgacg agcacgatgg ttgccgacaa
      661 ccacatccag accatgaatg ctatgacagc accgagggag ccgtaggtct cattataggt
      721 accgaaactt gagacgtacc aggagaacag catcgagcct gcgagccaga cgacggccgt
      781 gaagatgctg cccggggtga cccatttcca ctggggcttt tcccggcacg gcccgtagcg
      841 gtagagcagc gccagcgcca ggagcagggc gacgagcaac aggggccagc ggagcagtga
      901 gataatccgc ccggacatgc tctccaggcc aacgaaggcg aacatcgccg gaagaatgac
      961 gatgccgctc accgcaagga tgatgaacag aataccgccc agcgtgaaaa gcagcgcatg
     1021 caaattgtac tgcaggaagc tgcgccgctc cggctcttcg tagacgatgt tgagcgcatc
     1081 gaagacggat ttgaccccgg cgttggcgct ccataacgag acggcaagac caatgaagaa
     1141 gtagaggctc agcgtccgac cgccctgggc ctgaatgcgg ctgatctgat ctccaatgat
     1201 ctgcagcgct tcctcgggaa cgactccctg aagattggag agctgctgct ggatgctcgc
     1261 cggatcggcg acgaggccga agagtgagac gagggcagcg agggccggga atagggccag
     1321 cagggcgtaa tagctcacgc ccgctgcgac gagcatcacg cggtgttcct ggatccgctg
     1381 gtagacgcgc ttggcaatgt cccaccatcc gcgagccgga atgtgcgttg gagtgggggc
     1441 gtcttgcccg cgcgtggtgg catctgcgct gtttgccggc gcattcatat cggacagaga
     1501 cttggtctgt cctaaagagc cttctgttgg cggaccgtcg taagcagccc cctggtgatc
     1561 cgccgatggc gctcttctgc cgcgcaacgc catcgcccct agaacgacca gtccaagcag
     1621 gaaattgccc cgcccgcgcg tccggaagcg ggccccctgc ggtccggcgc tggcttcgtg
     1681 tttgtttccg ctcgctgtca tggctgaggg caaacgccct caccagcagg ccgttccctg
     1741 ctatggatgg cactccggcc ttgcattctg agcgaacacc cgctaaatgc gccgtcattc
     1801 cacacgcgga tcttcgcgct ccggcggatt gaaagccccg ccgatggcgc tccggtgtgt
     1861 tttgccgttt cgttctgtgc cggcaaaaca cgtactggtc cgcggaggtg caaccggaaa
     1921 acgagagagt tccatgctgc caacgttcac gatgcgccag ctgctggaag ctggcgtgca
     1981 tttcggtcac caggctcatc gctggaatcc gaagatggcc gagtacatct tcggccagcg
     2041 caacaagatc cacatcgtca acctcgagcg cacgctcgag aagtacaacg acgcgatgaa
     2101 gtacgtgcgc cagctcgccg cgcgcaaggg aacgatcctg ttcgtcggca ccaagcgcca
     2161 ggcgcgcgac atcatcgccg aggaagcgcg ccgcgcgtcg atgcccttcg tcgaccagcg
     2221 ctggctcggc ggcatgctga ccaacttcaa gacggtcaag cagtcggtca agcgcttgaa
     2281 ggacctcgag acgatggccg aggacggcac gttcgaccgc atgagcaagc gcgaggcgct
     2341 gagccacaag cgcgagctga c
//
LOCUS       DASUMG010000329         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_283825, whole genome shotgun sequence.
ACCESSION   DASUMG010000329 DASUMG010000000
VERSION     DASUMG010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283825"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFX46_05410"
     CDS             complement(<1..329)
                     /locus_tag="VFX46_05410"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041751522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEX5600049.1"
                     /translation="MRALVLFSGGQDSTTCLAWALSRYDHVETIGFAYGQRHEVELIA
                     RRRVLEGLRSGFPGWAQRLGEDHVLDLAEFGAISETALTREAAIEMTAAGLPSTFVPG
                     RNLVFFT"
     gene            complement(334..702)
                     /locus_tag="VFX46_05415"
     CDS             complement(334..702)
                     /locus_tag="VFX46_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX5600050.1"
                     /translation="MSEGREARTYTGGCHCGKVRYEVTTALEPVLDCNCSICQKRGAF
                     WTYVEPSAFKLLSGEDDLTDYQFNKRRIHHLFCSTCGVSSFARGKGEDGKEGIAINVR
                     CLDGIDLASLKLTPFDGRSL"
     gene            850..2136
                     /gene="eno"
                     /locus_tag="VFX46_05420"
     CDS             850..2136
                     /gene="eno"
                     /locus_tag="VFX46_05420"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5600051.1"
                     /translation="MTAIIDITGREILDSRGNPTVEVEVVLEDGSMGRAAVPSGASTG
                     AHEAIELRDGDASRYLGKGVQKAVEAVNGEIFRALRDRDATDQRGIDQTLIELDGTPN
                     KGRLGANAILGVSLAVAKAAAEACTLPLYRYIGGTAAHLLPVPMMNIVNGGAHADNPI
                     DLQEFMIMPVSAPTCADAIRLGSEIFHTLRKALKDAGHNTNVGDEGGFAPNLQSADEA
                     LGFIMRAIEKAGYKPGEDVVLALDCASTEFYKDGKYNLAGEGKVLDSEGMARYLADLV
                     SRYPIVSIEDGMAEDDWQGWKLLTSELGDRCQLVGDDLFVTNTKRLAQGIAEGVANSI
                     LVKVNQIGTLTETLDAVAMAQRAAYTAVISHRSGETEDATIADLAVATNAGQIKTGSL
                     SRSDRLAKYNQLIRIEEDLGDAARYAGRSVLRRTTA"
     gene            2262..>2546
                     /locus_tag="VFX46_05425"
     CDS             2262..>2546
                     /locus_tag="VFX46_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5600052.1"
                     /translation="MRRIRQHLLVLLGCLLISAYFIHHTITGKHGLEARSQLQERSRA
                     LDREIAALEAVAAELLRDVLLLRPDPPHPDMVEEIAIGVLGMARPGDRIVL"
BASE COUNT          486 a          789 c          822 g          449 t
ORIGIN      
        1 taggtgaaaa acactaagtt gcggcccggg acgaaggtgg acggcaaccc ggccgcagtc
       61 atctcgatcg cggcctcgcg ggtcaatgcc gtttcactga tggcgccgaa ctcggccaga
      121 tcgagcacgt gatcctcgcc cagccgctga gcccaaccag gaaagccgga gcgaaggccc
      181 tccagcaccc tccgacgggc gatcaactca acctcgtgac gctggccgta agcgaatccg
      241 atggtctcaa cgtgatcgta gcgggagagg gcccacgcga gacacgtcgt cgaatcctgc
      301 cccccggaga acaggaccag cgcccgcatt gtcctacaag ctccggccgt cgaacggcgt
      361 cagcttcagg ctggcaaggt cgatcccgtc gaggcagcga acgttgatgg caatgccttc
      421 tttgccgtcc tcgcctttac cgcgggcgaa ggaggaaacg ccgcaggtcg agcagaaaag
      481 gtgatgaatt ctccgcttat tgaactggta atccgtgaga tcgtcctcgc cagagaggag
      541 cttgaacgcc gacggctcga cataagtcca aaaggcgccg cgcttctggc agatggagca
      601 gttgcagtcg agcacgggct caagcgccgt cgtcacctcg tagcggacct ttccgcagtg
      661 gcagccgccc gtgtaggtcc tggcctcgcg tccttcgctc atggcgcttg ctcctgacgt
      721 tgcagagaca gccgcgcgat agcgtctcca ccacaacggc gcaagcggct cttgtcaatg
      781 cagatgagtg ggttagaacc cgcccgcgag ttgccgcgta gttctcaaga gcttgagcag
      841 gaggtcctta tgacagccat catcgatatc accggccggg aaattcttga tagccgcggc
      901 aacccgacgg tcgaggtcga ggtcgtgctg gaggatggct cgatgggccg tgcagccgtg
      961 ccgtcgggcg cctcgacggg tgcgcacgag gccatcgagc tgcgcgatgg ggacgcatcc
     1021 cgctatctgg gcaagggcgt gcagaaggcc gtggaggcgg tcaacggcga gatctttaga
     1081 gccctacgag accgcgatgc gaccgaccag aggggcatcg accagacact catcgagctc
     1141 gatggcacgc cgaacaaggg gcggctcggg gctaacgcaa tcctgggggt atcgctggcg
     1201 gtggccaagg cggctgcgga ggcctgcacg ctcccgcttt accggtacat cggcggcacg
     1261 gctgcccatc tgctgccggt gccgatgatg aacatcgtca acggcggcgc gcatgccgac
     1321 aatccgatcg accttcagga gttcatgatc atgccggtgt cggcgccgac gtgcgctgat
     1381 gccatccgtc tgggcagcga gattttccat accctgcgca aggcgcttaa ggacgccggc
     1441 cacaatacga atgtcggcga cgagggcggc ttcgcgccaa atctgcaatc cgcggacgag
     1501 gcccttggct tcatcatgcg agccatcgaa aaggccggtt acaagcccgg tgaggatgtc
     1561 gtgctcgcgc tggattgcgc gtccactgag ttctacaagg acggcaagta caacctggcg
     1621 ggcgagggca aggtgctcga ttcggagggc atggcccggt acctcgccga tcttgtgagc
     1681 cgctacccga tcgtgtccat cgaggatggc atggccgagg acgattggca gggctggaag
     1741 ctgctgacct cggagctagg cgatcgctgc cagctggtcg gggacgacct gttcgtcacg
     1801 aacacgaagc ggctggcgca aggcatcgcc gagggtgtcg ccaactccat tctggtgaag
     1861 gtcaaccaga tcggcacgct gacggaaacc ctggacgcgg ttgcgatggc ccagcgtgcg
     1921 gcctatacgg ccgtcatctc ccatcgctcc ggcgagaccg aggatgccac catcgccgac
     1981 ttggcggtcg cgacgaacgc cgggcagatc aagacgggat ctttgagccg ctccgatcgc
     2041 cttgcgaagt acaaccagct catccgcatc gaggaggatc tgggggacgc ggctcgctat
     2101 gccggccgca gcgtgctgcg ccgcacaacg gcctgaggtt cgttttttgg cggcacacgg
     2161 agcatcactt tacccgctct taacgccgac gggtctagaa cgcgagcgtt gcaattgagc
     2221 acgtctcgat ggttctgcgt cggacatctc cggatcgagg gatgaggagg atacggcagc
     2281 atcttctggt actgcttggc tgcctactga tttcagccta cttcatccac cacacgatca
     2341 cgggcaagca cggcctcgag gcacggtctc agctccaaga gcgttcccgc gctctggacc
     2401 gggagatcgc tgccctcgag gccgtcgcgg ccgagctgct tcgcgacgtt cttctgcttc
     2461 ggcctgatcc accgcatccc gatatggtcg aagagatcgc catcggtgtt ctcgggatgg
     2521 cgcggcccgg tgaccggatc gttctg
//
LOCUS       DASUMG010000330         3043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_204202, whole genome shotgun sequence.
ACCESSION   DASUMG010000330 DASUMG010000000
VERSION     DASUMG010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204202"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VFX46_05430"
     CDS             <1..530
                     /locus_tag="VFX46_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX5600053.1"
                     /translation="TRSIFEELISSLGDDGGIVDLPDIFAEAWDAQRMIMAADMAHRF
                     SKLVERGSPDASSKQLRELLDEGRRVTAVDYLAAHDLRPRLHASLAPIFDYYDAIITP
                     ASIGSAPASLETTGDPVFCTLWTLLGVPALSLPVLQAPDGMPLGLQLVGPPNDDARLL
                     RSARVLLENLSKLES"
     gene            complement(551..739)
                     /locus_tag="VFX46_05435"
     CDS             complement(551..739)
                     /locus_tag="VFX46_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600054.1"
                     /translation="MADAISAIVGMALMIAFVVAIVGKVGETPLWIVSILAFVLMIVA
                     FWQDAFAPLLRRSRNNGR"
     gene            851..1204
                     /locus_tag="VFX46_05440"
     CDS             851..1204
                     /locus_tag="VFX46_05440"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX5600055.1"
                     /translation="MIAVIFEVTPAEGRKQEYLDLAAELRPELEKMDGFISIERFQSL
                     TDPGKLLSLSFWRDEESVARWRNYPGHRRTQARGRSGVFAGYRLRVANVMRDYEMTDR
                     REQAPEDSRAIFDKV"
     gene            1488..1772
                     /locus_tag="VFX46_05445"
     CDS             1488..1772
                     /locus_tag="VFX46_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600056.1"
                     /translation="MSRRRRHVAEGSADLPQAIPIEPAFGNSAPDGPAVGPQLTPADV
                     VVTTFVIEDEKTNCSRHPVDEPRIDDEDAWRRSTLAGQPSIEHVPRAAAA"
     gene            2136..2828
                     /locus_tag="VFX46_05450"
     CDS             2136..2828
                     /locus_tag="VFX46_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5600057.1"
                     /translation="MKARDIMSKNVITVGRETSVHDIAQLMTSKRISGVPVVAEDGTI
                     LGIVSESDLLRRAELGTEPQHKWWLSFFSDPDLLARQYRKTHGLKAEDVMSRRVVTVD
                     ENADLGEIANTLERRRVKRLPVVRDGKLVGIISRADIVKALSQSEPQPGKRISSDTEL
                     EEALLAKIREQDWLDGSFLNVTVQNGVVELNGFIPSAEQRRALHVLVDEVDGVQKVED
                     RLVIGSPTLRGV"
BASE COUNT          625 a          913 c          923 g          582 t
ORIGIN      
        1 ccactcgcag cattttcgag gagctcattt ccagcctagg tgacgacggg ggaatcgtcg
       61 atcttcccga tatttttgct gaggcgtggg acgcgcagcg aatgatcatg gctgccgata
      121 tggcgcaccg tttctcaaaa ctggtcgagc gcggcagccc ggatgcgtcc agcaagcagc
      181 ttcgcgagct tctcgatgag ggtcgccggg ttacagcggt agattatctc gccgcgcacg
      241 atctgaggcc acgtctgcac gccagcctgg ccccgatctt cgactactac gatgccatca
      301 taacgccggc gagcattgga agcgctccgg catcgctcga aacaacgggc gatccagtgt
      361 tctgcacgct ctggactctt ctcggcgttc cggcgttgtc tctgccggtt ctacaagctc
      421 ccgacggtat gccgctcggt ttgcagctcg tcggtccgcc taacgacgac gcgcgcttgc
      481 tacgttctgc gcgcgtcctg ttggaaaatc tctcgaagct cgaaagctga tcgaccaggg
      541 tcagctgacg tcagcgcccg ttgttccggg agcgccggag cagcggggcg aaggcgtcct
      601 gccagaacgc cacgatcatc aggacaaatg ccaagatcga tacgatccag aggggcgtct
      661 ccccaacttt ccctacgatc gcgacaacga aagcaatcat cagggccatg ccgacgatcg
      721 cggaaatagc atctgccatt gtctcttcct cgcccccggg caatgatgtc accagcgcat
      781 ctcggatgat attgcatctg ctcgccgatg caaagcgcga ggtgcgcaag gcctgcccgg
      841 aggttcaatc atgatcgcgg tgattttcga agtgacgccc gccgaggggc ggaagcagga
      901 atatctcgac ctggcagcgg aactcaggcc cgagctggaa aagatggacg gcttcatctc
      961 gatcgagcgc tttcagagcc ttaccgatcc tggaaagctc ctgtcgctat cgttctggcg
     1021 ggatgaggag tctgtcgccc gctggcgcaa ttacccaggc caccgcagaa cacaggcccg
     1081 tggccgctcc ggcgttttcg cgggttaccg gctgcgggtc gcaaatgtca tgcgcgacta
     1141 tgaaatgacg gatcggcgcg agcaggcccc ggaggacagc cgcgccattt tcgacaaggt
     1201 ttgattggaa acagggccgt cctgcaagaa gcgcccttcg aaaggctcga gccgcgtctt
     1261 aggcggctcg atgctcacgg tcgtggcgaa taccggatcg cctaacgccg agcagcgcgg
     1321 ttcctgcccg tccccaccag cctcgggccg ttcaagagag ctggtgcggt tgcgcccgcc
     1381 gacctttggg cctgattgac tggctttctc gtcggcgcgg gcgttctggc cgcgattgct
     1441 tgattgaagg tccgctcgag attctggcag cggctccgag ccgaaggatg agtcggcgtc
     1501 gccggcatgt tgccgaagga tcggcagacc tgcctcaggc cattcctatc gagccagcct
     1561 tcggcaactc cgcgcctgac ggcccagctg tcggccctca actcactccc gccgacgtgg
     1621 tggtgaccac attcgtgata gaagacgaaa agacgaactg ttcgcggcat ccggttgatg
     1681 agccgcggat tgatgatgag gacgcctggc gccgcagcac cctcgctggg cagccctcca
     1741 tcgagcacgt tccgcgcgcg gccgcagcgt agactgcgac ctgccatcga aagacttcca
     1801 tcccgctgga gcttgaggcc cccgctggag gccggtgctg cctcagcggt cgaaaggata
     1861 ctgaaaaggg ctgcaaccgc gctggcaagg ccgagcagcc gcactggata cttcaccgta
     1921 ctcccccgtt gacgagattg ccactcaacg catgagtgaa atgttgcata cacatttgtg
     1981 ccagaattgc ggcaatcgca ggttggcact cgattttcgg ttacaaccta tcggtgggaa
     2041 cgaggcggcg accggcgtgt tgatgagtat caatgtcgtt gcacagaact cggacgatca
     2101 ttcatccgcg taaacccaag ctcagggagg ctgccatgaa ggctcgcgac attatgagca
     2161 agaacgtcat caccgttggt cgggagacat cggtacatga cattgctcag ctgatgacca
     2221 gcaagcgaat cagcggcgtt cccgtcgtgg cagaggatgg cacaatcctt ggaatcgtca
     2281 gcgaaagcga cctcctccgg cgagccgagc tcggtactga gccgcaacac aaatggtggt
     2341 tgagcttctt cagcgatccc gacttgctgg cgcgccaata caggaaaact cacggcctca
     2401 aggcagagga cgtcatgtcc cgccgcgtcg ttacggtcga tgagaatgcg gacttgggcg
     2461 agattgccaa cactctggaa aggcggcgcg taaagcgcct cccggttgtg cgggatggca
     2521 agctggtcgg cattatttct cgtgctgaca ttgtgaaggc cctgagccaa agcgagccac
     2581 agcccggcaa acgaataagt agtgacactg agctcgaaga agccctattg gcaaaaatcc
     2641 gcgagcaaga ctggctggac ggcagttttc tcaacgtgac cgtacagaat ggcgtcgtcg
     2701 agctgaacgg cttcatcccc tccgcagagc agcgccgagc tttgcatgtt ctcgtcgacg
     2761 aggtggatgg agttcaaaag gtcgaggaca ggcttgtcat cgggtcaccg acattgcgag
     2821 gagtgtgagc gcaaggaggc gcgacgtaat ccgggctcgt cgatgcggta agcacgtgga
     2881 tgctccaacc cgacagccag gtacagcgcc tttcacaagc tcttcatcgc ctcggaatct
     2941 gccacgcgga tcagtcggca ccgcgacagc ggcattgaga gcctcccttg gtgcccggat
     3001 cggctcgcac aaatcagagg gcggataacc tcgtaagagc ttc
//
LOCUS       DASUMG010000331         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_124142, whole genome shotgun sequence.
ACCESSION   DASUMG010000331 DASUMG010000000
VERSION     DASUMG010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124142"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VFX46_05455"
     CDS             complement(<1..245)
                     /locus_tag="VFX46_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600058.1"
                     /translation="MPKSRDRSYEDFIWAEATRPELDRLTGNQRFWRSLEDLDGSKLV
                     DSCEEEHSGPPDSARVNRRALLRFMAASTVLGGLSGCA"
     gene            432..794
                     /locus_tag="VFX46_05460"
     CDS             432..794
                     /locus_tag="VFX46_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600059.1"
                     /translation="MDLFSLAHLIDAPMARLARNSVAYVHAVMKIDESWKPMHRRPPD
                     RLAECIAGAQWLQDSGALQNLRMAGEAGLSGRHAGVRIPLRGIMAESTIYSDAGNVKV
                     MRKWDGLHCALTDTPEPI"
     gene            complement(1153..1635)
                     /locus_tag="VFX46_05465"
     CDS             complement(1153..1635)
                     /locus_tag="VFX46_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600060.1"
                     /translation="MKEARIGAAESYGNGNSSHSEELEQPEMAAERFAREAGEQISKA
                     SEKAWSRLTPQVLGWSATAPAEQLEKMMQQFWSFGSNGEANRYARALAQTNVEMIGLV
                     GRRSRAYLDLPNHIAECRTPQQILEQQARFFQEMLHDYQATNERVMNCWMEVTSPSSR
                     "
     gene            complement(2170..>2987)
                     /locus_tag="VFX46_05470"
     CDS             complement(2170..>2987)
                     /locus_tag="VFX46_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600061.1"
                     /translation="PIDVVDRPNAKPLVVTRGEIRYENVSFHYGKDGGVIDSLSLRIG
                     PGEKVGLVGRSGAGKSTLVNLLLRFYDLEGGRILIDGQDIAEVTQDSLRAHIGMVTQD
                     TSLLHRSVMENILYGNPSAGEAAAIAAAKKAHAHDFIMELRDARGREGYAAHVGERGV
                     KLSGGQRQRVAIARVLLKNAPILILDEATSALDSEVEAAIQEQLYNLMEGKTVIAIAH
                     RLSTIAAMDRLVVMDRGRIVEQGTHGELLAKGGLYAALWARQSGGFLAKEAAE"
BASE COUNT          608 a          903 c          845 g          631 t
ORIGIN      
        1 gcacatcctg aaagcccgcc caataccgtg gaagccgcca tgaaacgcag cagggcccga
       61 cgattaactc tggccgagtc agggggaccg gaatgctctt cctcgcaact atcgacgagc
      121 ttgctgccat ccaggtcttc gagactccgc cagaaccgct gattgccagt taaccgatca
      181 agctctggac gtgttgcctc cgcccaaatg aaatcctcgt atgaccgatc acggctcttc
      241 ggcatgggct accggtggca aacggagcag tcggtgagag gatttgcgaa gacgtctctg
      301 acgcgaagcg aattttcttt cgccgtcgag ccagagggta cgttcgcgcg ggacagctgc
      361 tctggtgctg atgcggtggc gggtggttgt gaattccgat gacagctgag gcaccagctc
      421 atcgaaagcg aatggacctt tttagtttgg cgcatctgat cgacgcgccc atggcacgtc
      481 tcgcacgaaa ttccgttgct tacgtgcacg ccgtgatgaa aatagacgaa tcttggaagc
      541 cgatgcaccg acgaccaccg gatcggctgg ccgagtgcat cgctggtgcg caatggctcc
      601 aggacagtgg cgccctgcag aatttgcgaa tggcaggtga ggcaggtctg agcggtcggc
      661 atgccggcgt gcgcattccg ctccgcggta ttatggcaga aagcacaatc tattccgacg
      721 ccggaaacgt gaaggtcatg cgcaaatggg atgggctgca ctgcgctctc accgatacgc
      781 cagaaccaat ctgaacgcac gaagatataa ccaccggcta aactgccgag aacaacaaga
      841 accgttgcta taattccaag ccgaaacgca gtcgtggctc caggccggaa aagctgcgcc
      901 atcgaatgac taccgacttg gcccaagcaa ttgggcggcg cacatcacga gttgtccgtc
      961 ttcaaacccg gaaggcccgc gaccgtttca gggaggaaac aaacaagtgc ttgcgttgtt
     1021 ccgagcacga gtggccgact cccggcggcc ggcaagcatg cgaaagctgt ccagtgaatg
     1081 caccttcggg caacgacgaa tcgaagcctg atcagcgtct taagattggg gcctgcggat
     1141 gggaggcggc catcaacgcg atgacggcga tgtgacctcc atccagcagt tcatgacgcg
     1201 ctcgtttgtg gcttgatagt catgcagcat ttcctggaaa aatcgagcct gctgttccaa
     1261 gatttgctgc ggcgtgcggc actccgcgat gtggttcgga aggtctaagt aagccctgct
     1321 gcgtcgcccg accaatccga tcatctcgac gttggtctgc gcaagggcgc gcgcgtagcg
     1381 attggcctcg ccattcgatc cgaagctcca gaattgctgc atcatcttct caagctgctc
     1441 tgcaggtgca gttgctgacc acccgagcac ttgaggtgtc aacctgctcc aagccttctc
     1501 gctcgccttg ctaatctgct cgccagcttc tcgcgcaaac cgttctgccg ccatttccgg
     1561 ttgctccagc tcttctgagt gtgacgagtt cccgttgccg taggactcgg cggcgccaat
     1621 tcgcgcctcc ttcatctctt ccttcgaacg cctgccggaa gtgcggccct tcctcgacag
     1681 ctgatgccga ccctttctgc tctgagcgtg cttcggattc gctgccacga tcaacctccc
     1741 atgatgggtt gagcattgcc cggaccgccg agctccgagc aacttgtcta cttccctcaa
     1801 cctgagctca ttgacccggc tcaagcggat cctgcgaaaa cgcgagatct gccattacgg
     1861 cttgcccatg gatggacatc aacggacagc aaccttgctg ccgagttggc tagtaaaccg
     1921 aatggatgcc ttttggtttc cgtatgccgc cttatcggag tatttcaccg cacggcatcg
     1981 gatggtcagc gtgtcacgcg cgtactgaat gtacgtactc gctcgaagtt cactcgaaga
     2041 agaataggaa ccctggtagt tccttaaagg gtttgttggg cctcataggc tctgtctctg
     2101 tcctacaatc tgtcgatcac ggtgatgacc agggaaacgg cgggcaatcg ctcgtgtgcc
     2161 tttgccccac tactccgccg cctctttcgc gaggaacccg ccggattggc gtgcccaaag
     2221 agccgcataa agaccgccct tggccaacag ctcgccgtgt gtgccctgct cgacgatgcg
     2281 cccccggtcc atgaccacca aacggtccat ggcagcgatg gtggaaaggc gatgcgcgat
     2341 tgcaatgacc gtcttacctt ccatgagatt gtagagctgc tcctggatgg ctgcctcgac
     2401 ctccgagtcg agcgcgctgg tcgcctcatc gaggatgaga ataggcgcat tcttcagaag
     2461 gactcgggca atagccacgc gctggcgttg accgccggag agcttcacgc ctcgctcgcc
     2521 tacgtgagct gcgtagcctt cgcgcccgcg cgcgtctctg agttccatga tgaagtcgtg
     2581 ggcatgtgcc ttcttggctg ccgcgattgc tgctgcctcg ccggcacttg ggtttccata
     2641 aaggatattc tccatcaccg agcggtggag cagggaggta tcctgcgtca ccatcccaat
     2701 gtgagcgcgg agactgtcct gagtgacctc ggcgatgtcc tgcccgtcga tcagaatgcg
     2761 tccaccttcc aggtcgtaaa agcgtagcag cagattgacc aacgtcgatt tgccagcgcc
     2821 ggagcgaccg accagcccaa ccttctcacc ggggccgatc cgcagagaaa gactgtcgat
     2881 gacaccgcca tcctttccgt aatggaagct cacgttctcg tatcggattt cccctctagt
     2941 aacgacgagc ggcttggcat tgggccgatc gacgacatcg atgggcc
//
LOCUS       DASUMG010000332         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_43301, whole genome shotgun sequence.
ACCESSION   DASUMG010000332 DASUMG010000000
VERSION     DASUMG010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43301"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFX46_05475"
     CDS             complement(<1..655)
                     /locus_tag="VFX46_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX5600062.1"
                     /translation="MRRTDHRQLSFVLADSPEGGGSEGLPDASARRAFLLQRAKRKRS
                     ARTVAWAGDTSRLLEEVASEANLALALINVVRNKGAPGIDGQTVEEAEAAAPKLLTRL
                     RRALLEGRYRPGDVRRVFIPKPGGGQRGLGIPNVIDRIVQQAVLQVLEPVFEPTFHHS
                     SHGFRPRRGATTAIAEAKEYLGAGYRTVVDFDLAQFFDRVHHQRLLDRIGQRVADRRV
                     "
     gene            complement(1435..2466)
                     /locus_tag="VFX46_05480"
     CDS             complement(1435..2466)
                     /locus_tag="VFX46_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5600063.1"
                     /translation="MQVTTIGLDLAKNVFQVHGVDAAGKVIFQRQLRRGRVIEFFKKL
                     PHCLVGMEACATGHHWARELTKLGHTVRLMPASYVKAYVRRQKNDAADAAAICEAVTR
                     PSMRFVPVKSAEQQAALMLHRSRDLLVRQRTQLINALRAHLAEIGLVAATGVDGLKSL
                     LAIVHEAGESGELPSQMRQGLQALVAQLAALQEQIGTLEHGIHAQHRASEASQRLETI
                     PGIGVIGATAIAATITDPSAFKSGRELAAWIGLVPRQSSTGGKQKLGGISKQGDRYLR
                     RLLIVGATAVIRHARAHPEKHPWIMKLLAKMPAKKVAVALANKMARIAWAILAKGGTY
                     RAPALAAAA"
BASE COUNT          476 a         1045 c          887 g          570 t
ORIGIN      
        1 tcacccgacg atccgccact ctttgaccaa tgcggtcgag aaggcgctga tgatggactc
       61 gatcgaagaa ctgggccaga tcgaaatcca cgaccgtccg gtagccggct ccaagatact
      121 cctttgcctc ggcgatggcc gtcgtcgcac cgcgcctcgg ccggaacccg tggctgctgt
      181 gatgaaaggt tggctcgaac accggctcca acacctggag cacggcctgt tgcacgatcc
      241 tgtcgatgac attggggatg cccagccccc gctgcccgcc gccaggcttc ggaatgaaca
      301 cccgacggac atcgccgggc cggtagcgtc cctccagcag cgcacgacgg agccgagtga
      361 gaagcttcgg cgcagcggcc tccgcctcct cgaccgtctg tccgtcgatg cccggtgcgc
      421 ccttgttgcg caccacgttg atgagcgcca gagccagatt ggcctccgac gccacctctt
      481 ccagcagccg acttgtatcg cctgcccagg cgacggttcg tgccgatcgc ttgcgcttcg
      541 ctctttgcag caggaacgcg cgccgcgcgg atgcgtccgg cagcccttcg ctcccgcccc
      601 cttcagggct gtcggcgagc acgaagctca attgacggtg atccgtcctt ctcaacccat
      661 ctctcccttt gctctgccgg ctttcgccga cttcatcgct cctatgaaga gatccgactt
      721 ctgggcaggc gtcggccggt cgtcgtcgcc tccttccagc ctaccgctgt cgtgctgtcg
      781 ccagcgccgc aacggacccg tccagatctc cccgggtaat gacactggat gtcccgccgc
      841 cgccgctccc cttaccgcct cagcctcggt tggattttgg gcgtcgcgta tggaggcacg
      901 ctcacccggc tgagatggcc tgctcaggag ttcacttgcg ttcggtgctg cggttcgcct
      961 cgggcttctt ccccacacgg cctcgcggcg ccagcatcgc gcgtctcacg tcgggccatg
     1021 ctgcgtgcag ttgcctccgg ctcacggttg ctaccaactc gccccgtaaa ggacttccac
     1081 ctccaatcca gtgcccatgc cgggcacacc tccgcaaact ccggccttga gcagcggaag
     1141 aacgcacaca gtggaggggt catgtgtgga cggcgcccgc gttgcaagag gaatctggcg
     1201 tgcggcttgt ggtcgggtgc aagtcatgtg tccggcctgt tcgcgcggca cgtgaccgct
     1261 ggccctgatg tgatccgctg atcgggatcc aatcaaaatt ccgggctcga agcccataga
     1321 ccccgaagga gtcccccgat cgacggctcg accgtttcgc atcacgccat catcaccctc
     1381 gcaatttggt tgtcgcacgc ggccgatgcc tcaccctccc attgccgagc ctcttcaggc
     1441 cgctgccgcc agtgccggcg ctcgataggt cccgcccttg gccaggatcg cccacgcgat
     1501 gcgcgccatc ttgttggcaa gcgcgacggc gaccttcttc gccggcatct tcgcgagcag
     1561 cttcatgatc catgggtgtt tttcgggatg cgcccgcgca tgccggatga cggctgtcgc
     1621 gccgacgatc agcaatcgtc gcagatatcg atctccttgc ttcgatatac cgccgagctt
     1681 ctgtttgccg ccggtggagc tctgtcgcgg cacgagcccg atccaggccg cgagctcgcg
     1741 ccccgacttg aatgcgctcg gatcggtgat ggtcgccgcg attgcggttg caccgatcac
     1801 gccgatgccg ggaatggtct ctaggcgctg gctagcctcg ctggcgcggt gctgcgcgtg
     1861 aatgccgtgc tcgagcgtgc caatctgctc ctgcaaagcc gcgagctgag cgacgagcgc
     1921 ctgcagccct tgccgcatct gggagggcag ttcacccgac tcgccagcct cgtgcacgat
     1981 ggcgagcaac gacttcagtc cgtcgacgcc ggtcgccgcc accaagccga tttcggccag
     2041 atgcgcgcga agggcattga tcagctgggt tcgctgccgg acgagaagat cgcgcgagcg
     2101 gtgcagcata agcgcggctt gttgctcagc actcttgacc ggcacgaagc gcatgctcgg
     2161 ccgtgtcacg gcctcgcaaa tggccgctgc atcggctgca tcgttcttct gccgccggac
     2221 atacgccttc acatagctcg ctggcatcag tcgcacagtg tgcccgagtt tggtcaactc
     2281 gcgcgcccaa tggtgacctg tcgcgcacgc ctccatgccg acgagacagt gcggcaactt
     2341 cttgaagaac tcgatcacac ggccgcgccg cagctgacgc tggaaaatga ccttccccgc
     2401 ggcgtcgacg ccatgcacct ggaacacgtt cttcgcaagg tccagaccga tcgtggtaac
     2461 ctgcatgtgg acggccccct cagtgattcg ttccaacgat caccctatgg cacttcgatg
     2521 ccgggagcgg gcgccgtcca cctcatcaga attgtaaggg cggcctccgg gccgtaggcg
     2581 gacgacccga ctccgtccag gcccatggcc ggcacgcctt caaacgcccc tattcggcga
     2641 tcgtgctgct cacggttcgc cagtctccgc ccgattatcc aatctgtcag gagcatcctc
     2701 tggcgcgctc ggtgaacttg ccttccaggc gccacaggcc ttgcggcgcg ctgcatgccg
     2761 aggcagatgc atgatgaaca cacacccatc cgcgcagttc cgcacccgcc aggggcgagc
     2821 cggctcgcgg tctgtaacag ctggtgatcg cactgatcgc gagcgcgtgt gcaggctctt
     2881 gagctgggcc tcgcctcacc ggcgctggtg tggtttccgc tcaaaatgga gcgcgcgggc
     2941 ctgagacgat gctggacagc ctttcacgtc aagctcgc
//
LOCUS       DASUMG010000333         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_284752, whole genome shotgun sequence.
ACCESSION   DASUMG010000333 DASUMG010000000
VERSION     DASUMG010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284752"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFX46_05485"
     CDS             complement(<1..314)
                     /locus_tag="VFX46_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600064.1"
                     /translation="MLPTGILPVPPFATARMLALDLVINGLIIGVFYALMAIGLSLIF
                     GILKIVNFAHGEFYMVGAYTYTLIALAFGVPPWLALPTAFVAGVVLGLVVERLLMRPL
                     YSG"
     gene            complement(356..1546)
                     /locus_tag="VFX46_05490"
     CDS             complement(356..1546)
                     /locus_tag="VFX46_05490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600065.1"
                     /translation="MAVAAGLFAGGGAVAQEPIKIGLTVTLSPPGSVSQGVQVRDGAE
                     VAVKMINDAGGVLGRPLELIVEDTQGIPEKARAAVEKLITRDKVIAVTGEHQSSAALA
                     GIEVAHRYNVPYVNTNGWSDVIREKGYPQVFNPGNFNSRVAIAAAETIAAMGAKNVLA
                     FPENTDYGVGLAKLIGDVLKEKAPDVEYRYETLDRAAKDFLPALLPLKANPPDVIVEI
                     MLPPAAYILINQLYEQGIAPTSKTLIYDASGIADYPDFWQNVSDSAKYMLVFGLYHPK
                     MQLPELGRQVAEAYTAKTGFEPNRLLFQAADSVFLIAEAIKIAGSTDPDAMIKALEGI
                     KWTGARGEITFSTEKGGYKYHQWLDVPYVTFQITDVNQPVKDTKLVQEAGQPIDLSLI
                     ERPN"
     gene            complement(1956..2135)
                     /locus_tag="VFX46_05495"
     CDS             complement(1956..2135)
                     /locus_tag="VFX46_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600066.1"
                     /translation="MTELLLTPSFDTAAEDNVAPTGGEANTNKAAAAAKEHAVEGFIR
                     RLPKLWLAPARRYFF"
     gene            complement(2757..2867)
                     /locus_tag="VFX46_05500"
     CDS             complement(2757..2867)
                     /locus_tag="VFX46_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEX5600067.1"
                     /translation="MVLARLVSRTATLLFVPYLAWVTYAAILNIRIWQLN"
BASE COUNT          566 a          862 c          936 g          511 t
ORIGIN      
        1 ccggaataga gcggccgcat gagcagccgt tcgaccacca ggccgaggac gacaccggcc
       61 acgaacgccg tcggcaaggc cagccacggc ggcacgccga aggcgagcgc gatgagcgtg
      121 taggtatagg cacccaccat gtagaactcg ccgtgggcga agttcacgat cttgaggatg
      181 ccgaagatca gcgacagccc gatcgccatc agcgcgtaga acacgccgat gatcaggcca
      241 ttgatgacga gatcgagagc gagcatgcgg gcggttgcga atggcgggac ggggaggatg
      301 ccagtcggca gcaccctccc ctgaagttac ggaacgccct gactgggcaa gctgcttagt
      361 tcggacgctc gatcagcgaa agatcgatcg gctgaccggc ttcctgaacg agcttggtgt
      421 ccttcacggg ctggttcaca tcagtgatct ggaacgtgac gtagggcaca tccagccact
      481 ggtgatactt gtagccgccc ttctcggtgg agaaggtgat ctccccgcgc gcgccggtcc
      541 acttgatgcc ttccagcgcc ttgatcatcg catcgggatc cgtgctaccc gcgatcttga
      601 tcgcctcggc gatgaggaaa acgctgtccg ccgcctggaa cagcagccgg ttcggctcga
      661 agcccgtctt cgccgtatag gcctcggcca cctgcctgcc gagctcgggc agctgcatct
      721 tcggatggta gagcccgaag acgagcatgt acttggccga atcgctcacg ttctgccaga
      781 agtcggggta atccgcgatg cctgacgcat catagattag cgtcttcgac gtcggcgcga
      841 tgccctgctc atagagctgg ttgatcagga tgtaggccgc cggcggcagc atgatctcca
      901 cgatcacatc gggcggattg gccttgagcg gcagcagcgc cggcaggaag tccttcgccg
      961 cgcggtcgag cgtctcatag cggtactcga catcgggcgc tttctccttg agcacgtcac
     1021 cgatcagctt ggcgagaccc acgccgtagt ccgtgttctc agggaacgcg agcacattct
     1081 tggcgcccat ggccgcgatc gtctccgcgg cggcgatggc gacacggctg ttgaagttgc
     1141 ccgggttgaa gacctgcgga tagcccttct cgcgaatgac gtcggaccag ccgttggtgt
     1201 tgacgtacgg tacgttgtag cgatgcgcca cctcgatgcc cgcgagcgcc gcggagcttt
     1261 ggtgctcgcc cgtcacggcg ataaccttgt cacgcgtaat cagcttctcg accgctgcac
     1321 gggctttctc aggaatgccc tgcgtatcct cgacgatcag ctcgagcggc cgcccgagga
     1381 cgccgccggc atcgttgatc atcttcacgg ccacttcggc gccatccctc acctgcacgc
     1441 cctgggacac ggatccgggc ggcgagagcg tgacggtcag gccgattttg atgggctctt
     1501 gcgcgacggc ccctccgccg gcgaataagc ctgcggcgac agccaaggcc gcacaaccaa
     1561 aatatttaag tattgataca ggtcccacga cgttcctcca tttgacgctt cttcatcgac
     1621 gcgtttgggc tggatcgtag agcagcccct acggaaaagg aagcgggaac cagcaccgct
     1681 ttcttacgcc aactccatcg agaacatcct cgcggccatc cagcgtactg cgcgctagta
     1741 accggctgaa cgcatacaga ctttctcctt gatcgagaga tcgggcctcg ggtgcaagca
     1801 ctcgagaatg ttcagcagcg gccaacaaga acagcgcacg cgcgcttcaa gatgtgtcgg
     1861 aatgtcgcgc aaaatatgcg cgctgtatcg caccctctcg caagttgaat tggggccttt
     1921 cgccgaagct cgactacgga agacttcggc acgcgtcaga aaaaataccg gcgcgcaggc
     1981 gctaaccaca acttcgggag acgtcgtatg aaaccttcta cagcatgttc cttggccgca
     2041 gcagcggcct tgttggtgtt cgcttcacca cccgtgggcg caacgttgtc ctcagcggcc
     2101 gtatcgaagc tcggggtgag cagcagctcg gtcattcgga tacagggcgc cggcgggcag
     2161 caaggcggac aaaggggcgg cggaggcggt gggggtatgc aaagcggcca gggcggtggt
     2221 ggaggcacca tggagaacag gggtggcgga ggtggaatgt ctcagccccg gggtggtggc
     2281 ggaggaacca tcgaaaaaag aggcggtggc ggcagtatgt cgagaggcgg ccgcgggacc
     2341 tgggagggca ggaggggcgg tagaggcggt ggaaccttgg aacgtcgtag aggcggcggc
     2401 ggtggcatca tattggaaga gaggcggaga ccgcgcggct atcgcggccc gcgcttccgt
     2461 gagggcccca cgctcagatt ccggacgtcg tgcggctggc tgagagaacg ggcccgtgat
     2521 accggcagcc gctactggtg gcgccgctat cgtgattgcg tgcgtggctg atcacgcgaa
     2581 aagcgggcgt cgcaattgcg ccgttgctgg cctcggcgcc cgcttcatcg atttaatgag
     2641 agcatcctgg cagcgggccg cctgtcggcc tgctgccggc agctgacgca atctcccggc
     2701 cctttgaccg accacggatc gattcctccc agtaacaaaa atcgggagcg gctggatcag
     2761 ttcagttgcc agatccggat gttgagaatc gcagcatagg tgacccaggc caaatagggc
     2821 acgaacagca gcgttgcagt ccggctcacg aggcgggcga gcaccataaa tgcga
//
LOCUS       DASUMG010000334         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_365513, whole genome shotgun sequence.
ACCESSION   DASUMG010000334 DASUMG010000000
VERSION     DASUMG010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365513"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1661)
                     /locus_tag="VFX46_05505"
     CDS             complement(<1..1661)
                     /locus_tag="VFX46_05505"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX5600068.1"
                     /translation="MRCNPLRWLLGGVLIIGLLGIMNLQGVLARIEAELLQQSRTALD
                     EAGLGWAEVAFSGRDGLITGSAPDEQELRKAYEITSSIWGVREVRNRAGLLEEQKDYV
                     WRANLREGRLKLSGYVPNEATRRAIIGSAKATFPDLEIQDRMQLARGAPNTEVWLGGI
                     SFGLKQLSRLKPEAAVELSKEGLTVVGEAQDQDAYRALKSSLANGLPQGVALNEDRVI
                     APVVKPYVWGARLSSNQLQLVGYVPDQDAHEQILSAAQGAFPQKSIVDRMRIGSGEPR
                     DFVTAAVGALGKLALLEEGRVEIKANELRISGLAAQEETARQLRESLRDSIPRSIKVD
                     AEIEFKEPTLPKISPYTVGVLIDEPSLRLSGYVPDEEARKALLEVVRSRFPSLTVTDE
                     LQLGAGAPEGLLTCIGAGLSALAALGNGRLQMSDAQLTVEAVTDDEEKAEAVQNELRA
                     SIDRACELDLKLVVDVPPEPNLSWRAVAGDGQLVLEGQVPDEATKAELFRIAGELFPE
                     TRLIDGTQTAAASSKKWAKAGETALTILSRLRSGEARLEGQNLTVT"
     gene            2151..>2650
                     /locus_tag="VFX46_05510"
     CDS             2151..>2650
                     /locus_tag="VFX46_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5600069.1"
                     /translation="MQDVPTKLKKYQVYIDGKWCDAASGKTFETIDPYTGEPWALIPE
                     CDAVDVDRACEAASRAFESGPWPRMTQTERGRLLRKIAGLIEKHGEYLAQIEVKDNGK
                     LISEMAAQLKYLPEWYYYYGGLADKITGMVVPVDKPDHFTYTLREPVGVCAMIIPWNS
                     PLLLVG"
BASE COUNT          528 a          846 c          756 g          520 t
ORIGIN      
        1 gtgacggtca ggttctgacc ctcaagccgc gcctcgccgc tacgaagtcg tgaaaggatt
       61 gtgagagccg tctcaccagc cttggcccat ttcttggaag aggccgcggc ggtctgcgtg
      121 ccatcaatca gccgcgtttc cggaaacagc tcaccggcaa tgcggaacag ctcagccttc
      181 gtagcttcgt cgggaacctg cccctcaagc accagctgac catctcccgc cactgcacgc
      241 cagctcagat tcggctcggg cgggacgtct acaacaagct tgagatcaag ctcgcacgcg
      301 cggtcgatgg atgctcggag ctcgttctga accgcctcgg ccttctcctc gtcatccgta
      361 accgcttcca ccgtcagttg ggcatccgac atctgcagcc ggccgttgcc caacgccgca
      421 agtgcggaca gtccggcccc aatgcaggtg agtaagcctt cgggagcacc agcgccgagt
      481 tgaagctcgt cggtgactgt cagacttggg aagcgagatc gcaccacctc tagcaaagct
      541 ttgcgtgctt cctcgtcggg cacgtagcca gacaaccgca gcgacggctc atcgatcagt
      601 acgccaaccg tatacgggct gatcttcgga agcgtcggct ccttgaactc gatctcagcg
      661 tcaactttga tcgatctcgg aatactgtcc ctcaggcttt cgcgcagctg cctggccgtc
      721 tcctcttggg cggcgagccc ggaaattcga agctcattcg ctttgatctc gacccggcct
      781 tcctcgagca aagcaagctt tccaagcgca ccaacggccg ctgtgacgaa gtccctgggc
      841 tcaccagagc cgatccgcat gcggtcgact atgcttttct gagggaaagc gccctgagcc
      901 gcggacaaga tctgctcgtg cgcgtcctga tccggaacgt acccgacgag ctgaagctgg
      961 ttcgacgaaa ggcgcgcgcc ccaaacgtaa ggtttaacga cgggcgcaat gacccggtcc
     1021 tcattcaacg ctacgccctg ggggaggcca ttggccaacg aagacttgag cgcgcgatag
     1081 gcgtcttggt cctgtgcctc gccgacaacc gtcaaccctt ccttgctcag ctcgacggct
     1141 gcttcgggct tcagccgaga aagttgcttc agtccaaagc ttatgccacc gagccaaacc
     1201 tccgtgttcg gagcgccgcg ggccagttgc atccgatcct ggatttcgag gtcggggaac
     1261 gtggccttcg ccgagccgat gatcgcccgc cgcgttgcct cgttcggtac ataaccgctc
     1321 agcttcagcc ggccttcgcg gaggttggcg cgccaaacgt aatccttctg ctcctctaga
     1381 agaccggcgc ggttcctgac ctcgcgcacg ccccaaatag agctggtgat ctcatacgct
     1441 ttccgcagct cttgctcgtc gggcgcgctg cccgtgatca gaccatctcg gccggagaag
     1501 gcaacctccg cccagccgag cccggcttca tccagagccg tacggctctg ctgcagcagc
     1561 tcagcctcga tgcgggcgag gacgccctga agattcatga ttccgagcag gccgatgatc
     1621 agaacgcccc ccagaagcca gcgcaaggga ttgcatctca tgccgctctt gtctccctcg
     1681 tcccccgtga caggcgcgcg attttcctcg attgttcacc tgcttattgt gctcacgcca
     1741 gtcacgatca cgtggccgcg ctgtcgtcgc caccgttcgc ccggcggaac tatagtcaac
     1801 agatttattc aatggaagtt ggctgctgga cggagcgaca ctgtagagtg ccgcgtaaga
     1861 atagtgcggt tgcgaatact agagcggctc gatcacagtc gatgtacatt ttgaaatatt
     1921 cttcacgacc aagctgcccc gcacagtaga gcgatgccaa ttagaaattg cccagcaaag
     1981 aaggttgcag tgacgggcac gacaggtagg taagctcacc ctgcgccgcg caaggcgcgt
     2041 cgtattgaca atgattctcg ccctcctatc gtgagttgtg atgtgccact gcgcaccagc
     2101 tcgaaccgat ctctcaagag cacaggcgcg ccattcttgg agccaatccc gtgcaagacg
     2161 taccgaccaa gctgaagaag taccaagtct atatcgacgg taagtggtgc gatgccgcat
     2221 cgggcaagac gttcgaaacg atcgatccgt acaccggtga gccttgggcg ctgatccccg
     2281 agtgcgatgc cgtcgatgtc gaccgggctt gcgaggccgc gagccgcgca ttcgaaagcg
     2341 ggccatggcc caggatgacg caaacagagc gaggccgcct gctgcgcaag atcgccgggc
     2401 taatcgagaa gcacggcgag tacctcgcgc agatcgaggt gaaggacaac ggcaagctca
     2461 tctccgagat ggcggcgcag ctcaagtact tgccggagtg gtattattac tacggcggct
     2521 tggccgataa gatcaccggc atggtggtgc cggtcgataa gccggaccac tttacctaca
     2581 cgctgcgcga gccggtcggc gtctgtgcca tgatcatccc atggaactcg ccgcttctcc
     2641 ttgtcggctt
//
LOCUS       DASUMG010000335         2151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_44361, whole genome shotgun sequence.
ACCESSION   DASUMG010000335 DASUMG010000000
VERSION     DASUMG010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44361"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            612..809
                     /locus_tag="VFX46_05515"
     CDS             612..809
                     /locus_tag="VFX46_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600070.1"
                     /translation="MARKKEQANTEKKVERPGKPARGSRRMHPAGPHARPELTDEAAT
                     PGTGALPDANPDTRDVDPGVD"
     gene            874..1284
                     /locus_tag="VFX46_05520"
     CDS             874..1284
                     /locus_tag="VFX46_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5600071.1"
                     /translation="MDTNIPSWMWSEACEMLARAERLRQQLFHPQWAKARQPCWQPPV
                     DLLETDEEVLVFIALPGVDPEHVQAVIQDGDLVIAGTRVLPAAFRTAAIHRLELPQGR
                     FERRIRLPAGSYRAVRWSASQGCLLIRLEKAGEL"
     gene            1284..>2151
                     /locus_tag="VFX46_05525"
     CDS             1284..>2151
                     /locus_tag="VFX46_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX5600072.1"
                     /translation="MAEPEAPETPKVKEIPTLPEDALIILPVRDLVLFPRVVLPITIG
                     RQRSILAAQYAVREQRQIGILTQRDPEMADPAPVDLYRIGTVANIVRFVTTPDGTHNI
                     VVQGEERFHVTEFLSGWPFFVARVLRIPDAETRTPEIEARFLHLQRQAIEALELLPQA
                     PREMIATIQSVTSPGTLADLAVAYMDIPTAEKQEILETVNLAVRMEKVSKLLAHRIEV
                     LRLSQEISRQTKAALDQRQREMLLREQMAAIRRQLGETEDTKAQEMAELEQAIARAGM
                     PKEVEEHARKELR"
BASE COUNT          423 a          675 c          666 g          387 t
ORIGIN      
        1 cgttgtggga tttgcgctga ttttggcggg tgtaacacta acgagccgca aaccatgagg
       61 cttgcggctc gtgaaactgt cgatcccttc gacccagatt actgctgctg acccgaaggg
      121 ggtgtcgtcg tctcaggcgg agacgttttc ggtggagtgg tttgcggcgg actcgatgat
      181 tgaggcgccg tggtctgcgg agtcgtcgcc gttgttgtcg gctcccgtgg gaacaacaag
      241 gccacgagca gcacgaccac gacgaccgcg ccaatcaacc accatattcg gctatccatg
      301 ccggcctcca agatttcact tacgtctgaa cgaaaaacgt gcgcagggcg cggatgttcc
      361 ggacggcccc gatgtcgcac tacggaggat cggctcatat tcgcagtgca gcacacgctc
      421 gcctgtttca actccccacc gatcggcgag ttcctggcaa gcgtttggca actgccgcag
      481 gcaagggacc cattaacgaa cagacaagcg cctgcacctt agacccaaat caaagacact
      541 gaaccatacc cttgccagga cgttccgcat ctgagcggct accggtgcgg cgacttgggc
      601 tgggaaatac gatggcgcgg aagaaggagc aggcgaatac cgagaagaag gtcgagagac
      661 ctggtaagcc cgcgcgcggc tcgcgccgga tgcatccagc cggtcctcat gcgcgccccg
      721 agctgaccga tgaagcggcc accccaggca ccggtgccct tcccgatgcg aaccccgaca
      781 caagagacgt cgatcccggt gtcgattaag ttgaagtcgg ggaccggcat cgacatcttt
      841 catgcagttg cagttaacct cggacgagcc tcgatggata cgaacatccc gtcctggatg
      901 tggtcggaag cctgcgagat gctggcgcgc gccgagcgcc ttcgtcagca gcttttccat
      961 ccgcaatggg cgaaggcacg acagccttgc tggcagccgc cggtcgatct cctcgaaacg
     1021 gacgaggagg tgctggtctt tattgcgctg ccgggggtcg accctgaaca tgttcaagcc
     1081 gtcatccagg atggcgacct cgtgattgcc ggcacgcgag tgcttcccgc ggccttcaga
     1141 acggcagcca tccatcggct cgagctgccc caggggcgct tcgagcgccg tattcgcctg
     1201 ccggcgggca gctatcgtgc ggtccgctgg tccgccagcc aaggttgcct gctcattcga
     1261 ctcgagaaag ctggagaact ttgatggctg agccggaggc ccctgaaact ccgaaagtga
     1321 aggagattcc gactcttccg gaagacgctc tcatcatcct cccggtgcgc gatctcgtgc
     1381 tcttccctag agtcgtgctc ccgatcacta tcgggcgcca acgctcgatc ttggccgcgc
     1441 agtatgctgt tcgcgagcaa cggcagattg gcatcctgac gcagcgcgac ccggagatgg
     1501 cggaccccgc accggtggat ctctaccgca tcggcaccgt cgccaatatc gtccgtttcg
     1561 tgaccactcc tgacggcacg cacaacatcg tcgtccaggg cgaggaacgg ttccatgtca
     1621 ccgaattcct gagcgggtgg ccattcttcg tcgcgcgcgt gctgcgcata ccggatgcgg
     1681 agacacgcac gccggagatc gaggcccgct tccttcatct tcagcgccaa gccatcgagg
     1741 ctctggagct tctgccgcag gcgccccggg agatgatcgc cacgatacag agcgtgacct
     1801 caccgggcac gcttgccgac ctcgcggtgg cctacatgga catcccgacc gcggagaagc
     1861 aggagatcct cgagacggtc aatttggccg tccgcatgga gaaggtatcg aagttgctgg
     1921 cgcaccgcat tgaggtgttg cgcctgtctc aggagatcag ccgccagacc aaggctgccc
     1981 tcgaccagcg ccagcgcgag atgctgctcc gcgagcagat ggccgccatc cggcgccagc
     2041 tcggtgagac tgaggacacc aaggctcagg agatggccga gcttgagcag gccattgcgc
     2101 gagccggtat gccgaaagag gtcgaggagc atgctcgaaa ggagctgcgg c
//
LOCUS       DASUMG010000336         2827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1008443, whole genome shotgun sequence.
ACCESSION   DASUMG010000336 DASUMG010000000
VERSION     DASUMG010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1008443"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VFX46_05530"
     CDS             complement(<1..992)
                     /locus_tag="VFX46_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5600073.1"
                     /translation="MSHFLDRLTFFRRAQEPFSEGHGITTNEDRSWEDGYRNRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKGGIVTWETQQTDYPRTRPDLPNHEPRGCSRGASYSWY
                     LYSGNRVKYPLVRSRLLRLWREARRTLAPVAAWASIVEDPEKRAAYVKKRGLGGFVRA
                     TWDEVTEIIAAANAYTIRNYGPDRIFGFSPIPAMSMVSYAAGTRYLSLLGGVSMSFYD
                     WYCDLPPSSPQTWGEQTDVPESADWYNAGFLILWGSNVPQTRTPDAHFYTEVRYRGAK
                     SVVICPDYSEASKFADLWLSPKQGTDAALGMAMGHVILREFHVERQVPYFEDYAR"
     gene            complement(1010..>2827)
                     /locus_tag="VFX46_05535"
     CDS             complement(1010..>2827)
                     /locus_tag="VFX46_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015514 - nitrite efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015707 - nitrite transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HEX5600074.1"
                     /translation="VVRGIHGPITFRLETGLFAFTVLVFVLGFFMSLGKAAVYKHIPV
                     YYPNHVGSVGGVVGLVGGLGGFVLPIAFGLLNDLTGIWQSCFMLLFAIVSIALVWMHV
                     SIRIMERGVLEPELKKLPELPEMQEIHEPRHVGVLGPHLIEDWRPEDKEFWETKGRAI
                     ARRNLWISIPSLLLSFAVWMVWSVVVAKLPAAGFTYTTDQLFWLAALPGLSGATLRIF
                     YSFMVPIFGGRLWTTLSTWSLLIPAVGIGLAVQNPNTPYWIMLLLALTCGFGGGNFAS
                     SMANISFFFPKSEKGSALAMNAGLGNLGVSVAQFLVPLVITTGVFGWLGGEPVMVTEG
                     DRQVPLWLQNAGFIWVPFIIASAFAAWFGMNDLATARASFAEQSVIFQRKHNWIMCWL
                     YTGTFGSFIGYSAGFPLLAKIQFPEIDVLPYVFLGPLIGSLSRVSAGWVSDKYGGGRV
                     TLWVFIAMALGVLGVLYFLSQENFAGFFAMFLFLFFASGVGNASTFQMIPAIMRKEMD
                     RLMPRATAAERLQQAEKESAAIIGFTSAIAAYGAFFIPKGYGTSIVLTGGPEAALVVF
                     LLFYITCIVLTWAIYTRPGGLLYDVERGGDGMTPRPAEA"
BASE COUNT          653 a          872 c          843 g          459 t
ORIGIN      
        1 cgggcgtagt cctcaaaata cggtacctgc cgctccacgt ggaactcgcg cagaatgacg
       61 tgtcccattg ccatgccgag cgccgcatcg gtgccctgct tcggtgagag ccacaggtcg
      121 gcgaatttcg aagcttcgga ataatccggg caaatcacaa cgctcttggc gccgcgatag
      181 cgaacctccg tgtagaaatg cgcatccggc gtgcgcgtct gcgggacatt cgagccccac
      241 agaatgagga agcctgcatt ataccaatcg gccgactccg gaacgtcggt ttgctcgccc
      301 caagtctgcg ggctcgaagg cggcaaatcg cagtaccagt cgtaaaacga catcgagacg
      361 ccgcccagca gcgacagata acgcgtgccc gccgcatagg acaccatcga cattgcggga
      421 atgggcgaaa aaccgaaaat ccggtccggc ccgtaattcc ggatggtata ggcgttggcc
      481 gcggcaatga tctccgtcac ctcatcccac gtggcgcgaa cgaatccgcc gagcccgcgc
      541 ttcttcacat atgccgcacg tttctccgga tcttcgacaa tcgacgccca agcggccact
      601 ggcgccagag tgcgacgcgc ctcgcgccac aggcgcagca accgcgaacg caccagtgga
      661 tatttcacac gatttccgga gtagagatac cagctatagg aggcgcctcg ggagcagccg
      721 cgcggctcgt ggttcggaag atcggggcgc gtgcgcgggt aatccgtctg ctgcgtttcc
      781 cacgtgacga tgccgccttt gacatatatt ttccaagagc atgagcccgt gcagttcacg
      841 ccatgtgtcg agcgcacgat tttgtcgtgc tgccagcgat tgcgatagcc gtcctcccag
      901 gaacggtcct cgttcgtggt aattccgtgc ccctcggaaa agggttcttg tgcccgcctg
      961 aaaaacgtca aacgatcgag aaaatggctc atggatagca ctccgttttt cacgcttccg
     1021 cgggtcttgg ggtcattcca tcaccgccac gctcgacatc gtacaggaga ccaccgggcc
     1081 gggtgtaaat ggcccaggtg aggacgatgc aggtgatgta gaacagcagg aaaacaacga
     1141 gcgccgcttc aggcccgcca gtcagcacga tcgacgtgcc atagcccttg gggatgaaaa
     1201 atgcgccgta ggccgcaatt gccgacgtaa atccaataat ggcggcactc tccttttcgg
     1261 cctgctgcag acgctcagct gccgtggcgc ggggcatcag ccgatccatc tccttgcgca
     1321 tgatggcggg gatcatctgg aatgtcgatg cgttgccgac gccactcgcg aagaagagga
     1381 acaagaacat cgcgaaaaag ccggcgaagt tttcctggct aaggaaatag aggacgccga
     1441 gcacgccgag cgccatggcg atgaacaccc aaagcgtcac acggccgccg ccgtacttat
     1501 cggaaaccca ccccgcagat acgcgcgata gcgacccgat cagggggccg agaaacacgt
     1561 agggcagcac gtcgatctcg ggaaactgaa tcttggcgag cagcgggaag ccagccgaat
     1621 agccgatgaa cgagccgaac gtgccggtat aaagccagca catgatccag ttgtgcttgc
     1681 gctggaaaat caccgactgc tcggcgaacg aggcgcgcgc tgtggcgagg tcgttcatgc
     1741 cgaaccatgc cgcaaaggcc gacgcgataa tgaacggtac ccaaatgaag cccgcgttct
     1801 gcagccagag cggcacttgg cggtcgcctt ccgtaaccat gacgggctcg ccgccgagcc
     1861 agccgaacac accagtcgta atgacgagcg gcaccaagaa ttgagcgacg ctgacgccga
     1921 gattgccaag gcccgcattc atcgccagcg cgctcccctt ctccgacttt gggaagaaga
     1981 aggatatgtt ggccatagac gatgcgaagt tgccgccgcc gaagccgcac gtcagcgcga
     2041 gcagcagcat gatccagtac ggcgtattgg gattctggac cgcgagcccg atgcccactg
     2101 cgggaatgag cagtgaccag gtggacaacg tcgtccacag ccggccgccg aaaatcggca
     2161 ccatgaagga atagaagatg cgcagcgtcg ccccggataa ccccggtaag gccgccagcc
     2221 agaagagctg gtcagtagta taagtgaagc ccgccgctgg aagcttcgca acgacgacag
     2281 accacaccat ccatacggcg aacgaaagca gcagagaggg aatcgagatc cagagattcc
     2341 ggcgtgcaat tgcacggccc ttggtttccc agaactcctt gtcctcgggc ctccaatcct
     2401 cgatcaaatg cgggccaaga acgccgacat gacggggctc gtggatctcc tgcatctcgg
     2461 gcagctcggg gagcttcttc agctccggct cgagcacgcc gcgctccatg atgcggatcg
     2521 acacgtgcat ccacaccagc gcgatcgaga cgatggcgaa gagcagcatg aagcagctct
     2581 gccagatgcc ggtgagatcg ttcaggagcc cgaaagcgat cggcagcacg aaaccgccga
     2641 gcccgcccac gaggccgacg acgccgccca ccgatccgac gtgattcggg taatagaccg
     2701 ggatgtgctt gtagacggcc gccttgccta ggctcatgaa gaagccgagc acaaagacga
     2761 gcacggtgaa ggcgaagagc cccgtctcca gccggaaggt gatggggccg tggatgcccc
     2821 tcaccac
//
LOCUS       DASUMG010000337         2669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_125657, whole genome shotgun sequence.
ACCESSION   DASUMG010000337 DASUMG010000000
VERSION     DASUMG010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125657"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFX46_05540"
     CDS             complement(<1..734)
                     /locus_tag="VFX46_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX5600075.1"
                     /translation="MTMSERQIEAQSTDTYTILPGRADRGLLLLCEHAGNAFPPEYGT
                     LGLPDSQLKRHIAYDIGAAEVTRRLSEALDAPAVMTRYSRLLIDPNRGEDDPTLIMRL
                     SDGAIVPGNRVITPEEREKRLRLYYWPYHNAIDDVLDCCLATGTTPAILSIHSFTESW
                     KGISRPWHVGVLYGEDNRLAEPFLEALCAENDLIVGANEPYIGQLVGDTLWRHGTQRG
                     LAHAIIEIRQDLIRDERGQAAWAERLA"
     gene            913..2169
                     /locus_tag="VFX46_05545"
     CDS             913..2169
                     /locus_tag="VFX46_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEX5600076.1"
                     /translation="MFQAAVQRAHPARCLPPHLPATPPPGNGRIIVLGAGKASAAMAV
                     ATEQFYRERGLSDRLTGFVTTRHGYGLPTDLIEVVEAGHPVPDAASQAAAERALSLAQ
                     EAGPNDIVLVLLSGGASALWAAPAEGITLEAKQALTKELLKSGARIHELNCVRKHLSR
                     IKGGKLAAAAYPARLLTLAISDVPGDDPATIGSGPTTGDPTTLADARTVLSRYGISPP
                     PSISAALQNPDNETPKPGADVLANSEYRLVAAPKASLEAAAEIARANGYRVELLGDAL
                     EGEAREVAEQHAALAREWLAKGERVAILSGGELTVTVRGSGSGGPNQEYALSLVESLG
                     GTPGIAALAADTDGADGGAGNPDDPAGAIVLPDTQARALACNLKAATFLANNDSTTYF
                     RELGDLVVSGPTQTNVNDFRAILIDP"
     gene            2305..>2669
                     /locus_tag="VFX46_05550"
     CDS             2305..>2669
                     /locus_tag="VFX46_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HEX5600077.1"
                     /translation="MAATLLAANPAAADLKLCNASNSRVGIAIGYQDEKGWATEGWWN
                     IGAQTCEVLLKGAVPSRFIYVYAIDYDRGGEWSGTNYMCTHDKSFAIRDVTDCQRRGY
                     RRTGFFEVDTGDAKDWTIRL"
BASE COUNT          505 a          840 c          827 g          497 t
ORIGIN      
        1 gcgagccgct cggcccaggc cgcctgtcct ctctcatcgc ggatgagatc ctgccggatc
       61 tcgatgatgg cgtgcgccag tcctcgctgt gtcccgtgcc gccacagggt atcgccaacg
      121 agctgcccga tgtagggctc gttggctccg acaatcaaat cgttctcagc gcacaatgct
      181 tcgagaaatg gctcagcgag ccggttatcc tccccgtaca ggacaccgac gtgccacggg
      241 cgcgagatac ccttccaact ctccgtaaag ctgtgaatgg acaggatcgc aggcgtcgtt
      301 cctgtcgcca agcagcagtc gagcacgtcg tcgatcgcat tgtgataagg ccagtagtag
      361 aggcgtaggc gcttttcccg ttcctcgggt gtaatcacgc ggttgcccgg aacgatagcg
      421 ccgtccgaca gccgcatgat cagcgttggg tcgtcctcac cgcgattggg gtcgatcagc
      481 aaccgagagt aacgcgtcat gacggcgggc gcgtccaagg cctctgaaag tcgccgcgtc
      541 acctcggcag ctccgatatc gtaagcaatg tggcgcttga gctgactgtc cggcagccca
      601 agcgttccgt actccggagg gaatgcgttt ccggcgtgtt cgcaaaggag aagcagaccg
      661 cggtcagcac gtccgggtag aattgtgtac gtatcggtgc tctgagcctc gatctggcgt
      721 tctgacatcg tcatctgatt gggccgccgc tgttgcatgg ttcccatcat ccagccttga
      781 cgcagcaaag ccaatcccgc agatgagcgc tggtcggcat tgtgacgcgg aaacgcagcg
      841 agcgcggggt taccggtgaa cacagacgat cgtcagcaca gcaccggggt tcagcgccga
      901 atcctgctcg aaatgttcca ggcggccgtt caacgggctc accccgcgcg atgtctgccg
      961 ccgcatctgc ccgctacgcc tccccctggg aatggccgca taatcgtgct cggagcaggc
     1021 aaggcgtccg cagccatggc cgtggcaacc gagcaattct atcgcgagcg cgggctcagc
     1081 gaccgattaa cgggtttcgt gacgacccgg cacggctacg ggcttccaac cgatctcatc
     1141 gaggtggtcg aggcgggcca cccggtcccg gatgccgcca gccaagctgc tgccgaacgg
     1201 gcactgagct tggcgcagga ggctgggccg aacgacattg tcctcgtgct gctgtcggga
     1261 ggcgcttcgg ccctgtgggc agcgccagcc gaagggatca ccctcgaagc caagcaggcc
     1321 ctgaccaagg agttgctcaa gtccggcgcg cggatccacg agctcaactg tgtgcgcaag
     1381 cacctctcgc gcatcaaagg cggcaagctg gccgctgccg cctatcctgc ccgccttctc
     1441 actctggcga tttcagacgt gccgggtgat gatccggcaa cgatcggttc cgggccaacc
     1501 acgggcgacc cgacaacgct tgccgatgcc cgcacagtgc tcagccggta cgggatttcg
     1561 ccgcctcctt cgatcagtgc agcactgcag aacccggaca atgaaacgcc gaagcccggc
     1621 gcggatgtgc tcgccaactc cgagtacagg ctggttgcgg cacccaaggc ctcgcttgag
     1681 gctgctgcgg agattgcccg cgccaacggt taccgggtcg agctcctggg cgacgcgctc
     1741 gagggtgagg cccgcgaggt cgctgagcaa cacgccgcgc tcgctcgcga gtggctcgcc
     1801 aaaggagagc gggttgcgat actctcgggc ggtgagctta cggtcaccgt ccggggcagt
     1861 ggcagcggcg ggccgaacca ggaatatgcg ctgtcgctcg tggaaagcct agggggaact
     1921 cctggcatcg ctgcgcttgc cgccgacact gacggcgccg atggtggtgc cggaaatccg
     1981 gatgatccgg cgggggccat cgtgctgcct gacactcaag cacgcgcttt agcgtgcaat
     2041 ctcaaagccg ccaccttcct ggccaacaac gattcgacca cttactttcg ggagctgggc
     2101 gacctcgtcg tcagcggtcc cacgcagacc aatgtcaatg attttcgcgc catcctgatc
     2161 gatccctgat taaggaattc cgttcttctg catgccacat atcgaccttt cccgatgaaa
     2221 atggacacac aagcgagcgt tccagcttgg acttcgtggg cactcgaacg tcctcaacct
     2281 ctcctgacag gatcactcgt ctgcttggcg gctacgttgc ttgccgccaa ccctgcggcc
     2341 gcagatctca agctttgtaa tgcctcgaac agtcgagtcg ggatagcaat cggctaccag
     2401 gacgagaagg gttgggccac cgaaggatgg tggaacatcg gcgcgcaaac gtgcgaagtt
     2461 ctgcttaagg gtgcggtccc gagccgcttt atttacgttt acgcgattga ctacgatcgt
     2521 ggtggcgaat ggtccggcac gaactacatg tgcacccacg acaagtcgtt cgccatcagg
     2581 gacgtgaccg attgtcagcg gagagggtac cggcggacgg gtttctttga agtggacaca
     2641 ggcgacgcca aggattggac cattcgcct
//
LOCUS       DASUMG010000338         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_687156, whole genome shotgun sequence.
ACCESSION   DASUMG010000338 DASUMG010000000
VERSION     DASUMG010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687156"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VFX46_05555"
     CDS             complement(<1..711)
                     /locus_tag="VFX46_05555"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX5600078.1"
                     /translation="MKIAIIGVGAMGSVYAALLGSAGHEVWAIDQWKEHIDAIREKGL
                     RVEGASGDRTVRIEATTDPADVRDADLVIIATKDNGVVDAAHSALQVAKENAPILTIQ
                     NGLGSADKVAEIVGTKRVMLGVVGGFGASIRAPGHAHHNGMEFVRLGEMDGGLTPRLE
                     AVAEVWRSGGFRTLTFEDIHKMVWEKLICNVTYSGTCALTGLRVGEVQANPSAWSVAT
                     ACANEAYQVARAKGIALDF"
     gene            802..>2147
                     /locus_tag="VFX46_05560"
     CDS             802..>2147
                     /locus_tag="VFX46_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX5600079.1"
                     /translation="MRNRSARISFYRGTGREKMKADLRSGLSLQSYARAVRLALAALA
                     LVTGLALVIVNPTSGQQNSNGPVLVFDVKGVIGFTSVEHLQRSVERAKAENASLIIMR
                     IDTPGGLVSSTRDMIQTILASPIPIVGYVAPSGARAASAGTYLMYAAHLAAMAPATHL
                     GAATPIQMGAPGITPSPDEKKDKSSEPDAAERKLINDAVSYLRGLAELRGRNADWAEK
                     AVREGATLTSTQAQEEKVIDFIAADLREVLEKANGRTVTTSTGTVTLDTRDRRIIEVE
                     PTWHMQVLEVIANPNVAFILMLLGIYGIIFELLNPGALFPGVIGGICLILALTALTVL
                     PVNLGGVALLLLGIGLLAAEAFTPSFGVLGIGGIVAFILGGLFLFDPDEVDIQFEVSR
                     PLVIGAAIASAAILAGVVGMAVQSRKRTVQAGAEQLIGGTAEVVEWSGHEGRVRVHG"
BASE COUNT          397 a          643 c          668 g          439 t
ORIGIN      
        1 gaaatcgagc gcgatccctt ttgcccgtgc cacctgataa gcctcgttcg cgcatgcggt
       61 cgcgacgctc cacgcgctcg gatttgcctg gacctctccg actcgtagac cggtcaaggc
      121 gcaggttccc gagtaggtga cgttacagat caacttctcc cagaccatct tgtggatgtc
      181 ctcgaaggtg agcgttcgaa agccgccgga gcgccaaacc tcggccacgg cctccaagcg
      241 cggggtcagg ccgccgtcca tctcgccgag gcgcacgaac tccattccgt tgtggtgggc
      301 gtgaccgggg gcgcgaatcg atgcaccgaa gccgccgacg acacctagca tgacgcgttt
      361 ggttccaaca atttcggcca ccttatcggc actgccgagc ccattctgaa tcgtcagaat
      421 tggagcattc tccttcgcca cctgaagggc agagtgggcc gcgtcaacca caccattatc
      481 tttggtggcg atgataacga gatcagcgtc gcggacgtcc gctgggtcgg ttgtggcctc
      541 tatcctgacg gtgcggtcgc ctgaagcacc ttccactcgg aggcctttct ctctgatagc
      601 gtcgatgtgc tctttccact gatcgatggc ccacacctca tgaccggcgg agccaaggag
      661 tgccgcatag acggagccca tcgctccgac gccgataatc gcgattttca tggtatcttt
      721 cctccaaggc tgcgatccta caggcccggc ttggggttgg ccattgaatg gcgcgcgtgg
      781 cgctgggtca ggagggtgcc aatgcggaac cgtagcgccc gcatcagttt ctaccgtgga
      841 accgggcggg aaaagatgaa agcggacctc cgatcagggc tgtcattgca gagttatgcc
      901 cgtgcagtca ggcttgccct cgccgccctg gcactggtga cagggctcgc gctcgtaatc
      961 gtcaacccga ccagcggcca gcaaaatagc aacggtcccg tcttggtgtt cgacgtcaaa
     1021 ggcgtgattg gcttcacgtc ggtagagcac cttcagcgga gtgtcgagcg tgccaaggcg
     1081 gaaaacgcca gcctgatcat catgcgcatc gacacgccgg gcggcctggt ttcctcgacg
     1141 cgcgacatga tccagacgat ccttgcgtcg cctattccaa tcgttggtta tgttgcgcct
     1201 agcggtgcgc gtgcggcgag cgcgggcacc tacttgatgt acgctgccca tctcgcagcg
     1261 atggcgcccg cgacacacct gggcgcagcc acaccgatcc agatgggtgc ccctggcatt
     1321 acaccctcgc cggacgaaaa gaaggacaag tcctctgagc cggatgcggc cgagcggaaa
     1381 ctgatcaacg acgcggtctc ataccttcgc ggccttgccg agctcagagg ccggaatgcc
     1441 gattgggcgg aaaaagccgt gcgcgagggc gcgactctga cctcaaccca agctcaggaa
     1501 gagaaggtca tcgacttcat tgccgcggat ttgagggaag tgctcgaaaa agcaaacgga
     1561 cgcacggtca cgacgagcac tggcactgtg acgctcgata cgcgcgatcg gcgcatcatc
     1621 gaggttgagc cgacctggca catgcaggtc ttggaagtca tcgccaatcc gaatgtcgct
     1681 ttcattctga tgctgctcgg catctacggc atcattttcg agctgctgaa tccgggcgcg
     1741 ctgtttccgg gtgtcatcgg tggcatctgc ctgatcctcg ctcttacggc tttgactgtg
     1801 ctcccagtca atttgggcgg tgtggcgttg ttgcttttgg gaatcgggct gttagcagcc
     1861 gaggcattta cgccaagctt cggcgtgctg ggaattggcg gcatcgtggc attcattctc
     1921 ggaggattgt tcctgttcga tcccgacgag gtggatatcc agttcgaggt ctcccggcct
     1981 ttggtgatcg gcgccgctat cgcgagcgcg gcaattctcg caggtgtagt gggaatggcg
     2041 gttcagtcgc ggaagcggac ggtccaagcc ggtgccgagc agctcatcgg cggcaccgcg
     2101 gaggttgtgg aatggtcggg tcacgaaggc cgcgtgcggg tgcacgg
//
LOCUS       DASUMG010000339         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_687252, whole genome shotgun sequence.
ACCESSION   DASUMG010000339 DASUMG010000000
VERSION     DASUMG010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687252"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VFX46_05565"
     CDS             complement(<1..600)
                     /locus_tag="VFX46_05565"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5600080.1"
                     /translation="MKLISVYFRAIAQLASEWWLAAGLTFANVAIGIVQLAEPILFGK
                     VVDALAQGTPVFKLISLWAVVGIFGIVTGVTVAVASDRLAHRQRLAAMSRAFERTLTL
                     PISYLAERGTGATVRTIVAGTDALFATWLTFMREQLSAIVSVLCLVPTAIYMDWRLAS
                     LLAVLAVIYTILNVTVVYRTSDGQASVERYYVDVFGRVGD"
     gene            complement(646..1062)
                     /locus_tag="VFX46_05570"
     CDS             complement(646..1062)
                     /locus_tag="VFX46_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600081.1"
                     /translation="MDIPREPGSLPRVLLAKPRVKGNFDVMVKTGIRPSHNPATTVIL
                     KNPAITNLRQAGGEHLAKCGISYLARSADNRDEGQFVLDDIGRSGRFPGSAGPPATNN
                     RANVIFRALEAPSDSPTVRLVSRPLSGILREVRKRM"
     gene            1411..>2091
                     /locus_tag="VFX46_05575"
     CDS             1411..>2091
                     /locus_tag="VFX46_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HEX5600082.1"
                     /translation="MADDLCDPIRNASPGRPYVVAQLGQSLDGRVATPSGESRWINRD
                     AALDHLHRLRACVDAVVVGVGTVLADDPQLNVRRVTGSHPARVVIDPNGRLPTSAKCL
                     ADDGARRIVVRACQEPAPAGVEVIRLPVTDGRMDPKAIVSALFECGLKKLLIEGGPQT
                     ISRFIDANAVDRLHVLVAPLIIGSGPTGLTLEPICRLAEARRPRTCVHLLEDGDVLFD
                     CDLRSDGRK"
BASE COUNT          421 a          615 c          681 g          374 t
ORIGIN      
        1 gtctccaacg cggccgaaga catcgacgta gtagcgctcc acagaagcct ggccgtcgga
       61 cgtgcgataa acgacggtga cgttgaggat cgtgtagatc accgccagaa ccgcgaggag
      121 actggcgagc cgccagtcca tgtagattgc cgtcggcacc aggcaaagga cgctgacgat
      181 tgcggaaagc tgctcacgca taaacgtgag ccacgtcgcg aaaagcgcgt cagttccggc
      241 gacaatggtg cgtacggtgg cccctgtgcc gcgttcggca agatagctga tcggcagcgt
      301 cagcgttcgc tcgaaagcgc ggctcatggc ggcaaggcgt tgccgatggg ctaggcgatc
      361 cgaggcaacg gcgacggtca cgccagtgac gatgccgaaa atgccaacaa cagcccaaag
      421 cgaaatcagc ttgaaaaccg gggtgccttg ggcaagggca tcgaccacct tgccgaaaag
      481 aatgggctcg gcgagctgca cgatgccgat ggcaacgttg gcgaacgtca gccctgcggc
      541 aagccaccac tccgaggcga gctgcgcgat tgcacggaaa tagacggata tgagcttcat
      601 ggcgattccg tcgtcagggc cagcacaagg gtgctcgacc gatcctcaca tccgtttccg
      661 aacttcgcgc agaatgccgg agagcggccg ggacacaagt cgcaccgtgg ggctgtctga
      721 tggcgcttcc aaggcgcgaa agatcacatt tgctctgtta tttgtcgcag gagggccggc
      781 gcttccagga aaccggccgg agcgcccaat gtcatcgagc acgaactgac cttcatcccg
      841 gttatcggca gaacgagcga gataagaaat tccgcatttt gcgagatgtt cgccgcccgc
      901 ctggcggaga ttggtaatcg ccgggttctt caaaatcacg gttgttgccg gattgtgaga
      961 tggccgaatg ccggtcttaa ccatgacgtc gaagttcccc tttacgcgcg gtttggcgag
     1021 aagaacgcgc ggcaaactcc caggttctcg ggggatatcc atcggccgtc tgcgcgatag
     1081 cttgaagtcg cccctagttg gcgactgatt tgctcgatgg ggacaccggt ggccggtatc
     1141 cttcagccgg ggcacagtgc gggcgggcgc gttgaggtcg tcgggccaca atcgcatccg
     1201 gtgccagcaa acgatccctg tcaacgcgat gcatgttatg atggcaccga gttagcaccc
     1261 cacatccgag gagcctggtg gcatttccga ctcgtcagtg gtgtaaatgt tccgtacggg
     1321 tcggactggg catatcaccg cggcatcgcg gagagccaga aagaagcggg cttccgaggg
     1381 gtcgaggcag ggaacagcga agtgacgacg atggccgacg atctctgcga cccgatccga
     1441 aatgcgagcc ctggtcgtcc ctatgtcgtc gctcagctcg gccagtcgct ggacggccgc
     1501 gtcgccacgc cgagcggtga aagccgctgg atcaaccgcg acgctgcact cgatcatctt
     1561 cataggctgc gcgcgtgcgt ggatgcggtg gtagtcggcg tcggcacggt gctcgccgac
     1621 gatccccagc tgaatgtgcg ccgggttacg ggcagccatc cggcccgcgt ggtgatcgat
     1681 cccaatggcc gccttccgac cagtgcgaaa tgcctggctg atgacggcgc gaggcgcatc
     1741 gtcgtgcgcg cctgccagga gccggctcca gcgggggtcg aggtgatccg tttgcctgtg
     1801 acggacggca ggatggatcc caaggcgatt gtttccgcgc tcttcgaatg cggcctgaaa
     1861 aagctgctga tcgaaggcgg gcctcagacg atttcccgtt tcatcgatgc caatgctgtc
     1921 gatcgcttac acgtcctcgt cgcaccattg atcattggct caggccccac ggggctgacg
     1981 ctcgagccca tctgccgtct ggcagaggca cggcggccga ggacatgtgt gcacctgctc
     2041 gaggacggcg acgtcctgtt cgactgcgac ctcagaagcg acgggaggaa a
//
LOCUS       DASUMG010000340         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_527223, whole genome shotgun sequence.
ACCESSION   DASUMG010000340 DASUMG010000000
VERSION     DASUMG010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527223"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..1260)
                     /locus_tag="VFX46_05580"
     CDS             complement(289..1260)
                     /locus_tag="VFX46_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600083.1"
                     /translation="MGLQATPADAQKRDVSFRLDWIYQGPNAGFMVAKDKGFYDEAGL
                     NVDMGPGKGSGSTAQLIASKTSQFGFADGFVIGAGVAKGMELVMVASMYRRNPTAAIV
                     LTESGIKTPKDLEGKTIGIAPGGTQFQQWPAFVKGCKLDGDKIKVINVDPAGTVPALV
                     AGKVQAVASYAQGAVPGIEIRSKKPVTSFYYADCGVTAVSNGIIVHKDLLKQDPELIR
                     TFVKATLKGFLYSFQHPDEAVAITKKFAPEIVPEITKRELEMSFLNWQTPNTAGKQMG
                     WMSDKDWDSTVETLKLYGGVTTPLKASDLYTNEFIPAGSEFATPPVK"
     gene            1707..>2206
                     /locus_tag="VFX46_05585"
     CDS             1707..>2206
                     /locus_tag="VFX46_05585"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5600084.1"
                     /translation="MDRKTRIAVVGAGLGGMTVAGFLQRAGFSVTVYEQAPAFSRIGA
                     GIILSANVTKVLRRLGLEQMVVDIGIKADCYISRAWDTGETMYKIEFDAESEARFGGP
                     YANIHRGDLHAVLEKGVAPGSIAFNHRLVALDETRDAIGLVFENGARVEADIVIGADG
                     VNSKVR"
BASE COUNT          379 a          705 c          671 g          451 t
ORIGIN      
        1 agaaactccc gcttgcgctg gccgatggaa ataccgtcga gcgcccggac cgggccatcg
       61 tcggtcgcgt agatcttcga cagctccctg aactcgagat agggcgattc ctccggctcc
      121 gaccgggccg tttcggccgg caggatccgt atgtcctttg cgacactagc catgcgttac
      181 atcggccaga gtgttgttgc acacgcttgc aggccgccta tcgtccacgg gacctcccga
      241 attccggtca gctcgcgcgc gccgcggttc gccatcccct tttggttgtt actttaccgg
      301 cggcgttgcg aattcgcttc cggctggaat gaactcgttg gtgtagaggt cggacgcttt
      361 cagcggcgtc gtcaccccgc cgtacagctt cagcgtctcc accgtcgagt cccagtcctt
      421 gtcggacatc catcccatct gcttgcccgc ggtgttggga gtctgccagt tgaggaacga
      481 catttcgagc tcgcgcttcg tgatctccgg cacgatctcg ggtgcgaact tcttggtgat
      541 ggccacggcc tcgtcgggat gctggaacga atagaggaat cctttcaagg tcgccttgac
      601 gaaggtgcgg atcagttccg gatcctgctt cagcaaatcc ttgtgcacga tgatgccgtt
      661 gctcacggcc gtgacgccgc agtcggcgta gtagaacgat gtgacgggct tcttggagcg
      721 gatttcgatc cctggaaccg cgccctgcgc gtagcttgca accgcctgca cctttcctgc
      781 caccagcgcg ggcaccgtgc cggcgggatc gacattgatg actttgatct tgtcgccgtc
      841 gagcttgcag cctttgacga aagccggcca ttgctggaac tgggtgccgc ccggcgcgat
      901 accgatcgtc ttgccctcca gatccttcgg cgtcttgatg cccgattccg taagcacgat
      961 ggcggcggtc ggattgcgcc ggtacatgga ggcgaccatg accagctcca tgcccttggc
     1021 gacgcccgct ccgatcacaa agccgtcggc gaagccgaac tggctggtct tgctggcgat
     1081 cagttgggcc gtgctgcctg agccttttcc cggtcccatg tccacgttga ggccggcctc
     1141 gtcgtaaaag cccttgtcct tggcgaccat gaagccggca ttcgggccct ggtaaatcca
     1201 gtcgagccgg aagctcacgt cgcgcttctg ggcgtctgcg ggcgtggcct gcagacccac
     1261 aagcgccagc cctgcgatcg cgtaacggat ggagcaccgg ccgggaagga cctgaaacat
     1321 gcgatgacct cctgtgcggg ggcactaggt tcccccaagc ggcaaatcac ggttttacat
     1381 tcaagtctcg tgccaaggac gcggattaca tctccaacca tgcggtcatt ttgacccacc
     1441 gcgcccggat catcagcgac ctcgcgggcc gtgtcccgcg ctccggcctc taccctgcgg
     1501 atacgttaca gcgtgcatcg ccaagtcgac actgtctctg atctgtgtcc gcgcagcgtc
     1561 atcgccgatc ccgcttcgag cgcaccgcgg atcgacttcg atcaacgcgc tcctggatca
     1621 cgatccatat ccatagccga agtttgcacg ttggcaaaaa aatctcgcgc atattaggct
     1681 cgatcatcgg gcacggcgga tcggcaatgg atcggaagac gcggattgcg gtcgtcggtg
     1741 ccgggcttgg ggggatgacc gtcgcgggct ttctgcagcg cgcgggcttt tcggtcaccg
     1801 tctacgagca ggcgcccgcc ttttcccgca tcggcgccgg aatcatcctc agcgccaacg
     1861 tgacaaaagt gcttcgccgt ctcggcctcg agcagatggt cgtcgacatc gggatcaagg
     1921 ccgactgcta tatcagccgg gcctgggaca ccggcgagac catgtacaag atcgagttcg
     1981 acgccgagag cgaggcccgg ttcggcggac cctacgccaa tattcaccgc ggcgacctgc
     2041 atgccgtgct cgagaagggc gtggcgcccg gatcgatcgc attcaatcat cggttggtcg
     2101 ccctcgacga gacgcgcgat gcgatcggcc tcgtattcga gaacggcgcg agggtggagg
     2161 ccgatatcgt catcggcgcc gacggagtga actccaaggt ccggga
//
LOCUS       DASUMG010000341         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_607135, whole genome shotgun sequence.
ACCESSION   DASUMG010000341 DASUMG010000000
VERSION     DASUMG010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607135"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..657)
                     /locus_tag="VFX46_05590"
     CDS             complement(127..657)
                     /locus_tag="VFX46_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX5600085.1"
                     /translation="MGLYKLDGLSVTTPGEGRYWVAENAIVVGNVHIGEDVSIWFNTV
                     IRGDNEPIEIGTGSNIQDGCVLHTDPGFPMIIGPDCTIGHMVMLHGCKIGRGSLIGIG
                     SIILNGAVIGEESLIGANTLIPEGKEIPPRSVVMGSPGKIVRQVTEADLVRMQAGAAF
                     YRKNWRRFSAGFSPQI"
     gene            complement(667..918)
                     /locus_tag="VFX46_05595"
     CDS             complement(667..918)
                     /locus_tag="VFX46_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HEX5600086.1"
                     /translation="MARSVRAAAKDGGIKQDIPRVQRYLRQLFHTDELRVVPHARKKD
                     MAEVFIGDEYIAPLYREEEDGEVSFQLQIAILEEDLEEA"
     gene            complement(918..1757)
                     /gene="cysE"
                     /locus_tag="VFX46_05600"
     CDS             complement(918..1757)
                     /gene="cysE"
                     /locus_tag="VFX46_05600"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEX5600087.1"
                     /translation="MAPTPRFQAQSKIAQVDPIWAEVRREAEEAMLAEPALGGFIFAT
                     VLSHERLENAVCHRLAQRLNHSDVDAGLIGQTFLDVLSRQPELGQAFRADLAAVYDRD
                     PACSRYLEPLLYFKGFHALVTHRFAHELWKEGRRDFALYLQSQSSRIFGVDIHPAARI
                     GIGIMIDHATGVVIGETATVGDNTSLLHGVTLGGSGKESGDRHPKIGASVMIGAGAKV
                     LGNIKVGNCSRIAAGSVVLKDVPPNTTVAGVPAKVVGPAGCPEPARSMDQRLNPDDCG
                     VES"
     gene            complement(2023..2499)
                     /locus_tag="VFX46_05605"
     CDS             complement(2023..2499)
                     /locus_tag="VFX46_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX5600088.1"
                     /translation="MSTSEQYLRQAIELAFDNIEKGGRPFGAVVVKDGKRIATGVNEV
                     MVTNDPTAHAEIMAVRAAAQALGTPDLSGCTVYASGQPCPMCMAAMRIAGVEEVVFAH
                     SSEDAEPYGLSRAAISADLAKPFSEQSMKIRHEPVRLESRPDLYGEWKRRQDGQAG"
BASE COUNT          514 a          861 c          757 g          512 t
ORIGIN      
        1 gctgaaagcg cggcgcttcc gtgaagaact gcttcgcggc catgtggccg gcaggaatcg
       61 ttacagctct gatggtcgcg cctcagagca atactttatt cgtcaaaatg ggaaagaagc
      121 gctgatctaa atctgcggcg agaaccctgc cgagaaccgg cgccagttct ttcgatagaa
      181 tgcggctccg gcctgcattc gtacgaggtc agcctccgtg acctgccgga cgatcttgcc
      241 gggagacccc atcaccaccg accggggcgg gatctccttg ccttccggaa tcagagtgtt
      301 tgctccgata aggctctcct cgccaatcac agcaccgttg aggataatgg agccaatgcc
      361 gatcaggctg ccacggccga tcttgcaccc gtgcagcatg accatgtggc cgatggtgca
      421 atccggaccg ataatcattg ggaagcctgg atcggtatgg agcacgcagc cgtcctggat
      481 attcgagccc gtgccgatct caattggctc gttatccccg cggatcaccg tattgaacca
      541 gatgctcaca tcctcgccga tgtgaacatt gccaacaaca atggcattct ccgctaccca
      601 gtagcgaccc tcacccggcg tcgtaacgct cagcccatcg agcttgtaga gccccattct
      661 accccctcag gcttcctcga gatcctcctc gaggatcgca atctgtagct ggaacgaaac
      721 ctcgccgtcc tcctcctcgc ggtagagagg cgcgatgtac tcgtccccga tgaacacctc
      781 ggccatatct ttcttccgcg cgtgcggcac cacccgaagc tcgtccgtgt ggaagagctg
      841 gcgcaaatac cgctgcacgc gtggaatatc ttgctttatt ccgccgtctt tcgcggcagc
      901 ccgaaccgaa cgagccatca gctttcaacc ccgcaatcgt ctggattgag ccgctggtcc
      961 atggaccggg ccggctccgg acaccctgct ggacccacca ccttggccgg aacgccagcg
     1021 accgtcgtgt taggcggcac gtccttgagc acaacgctcc cggccgctat gcgcgagcag
     1081 ttgccgacct tgatgttgcc cagcaccttg gcaccggcac caatcatgac gctggcaccg
     1141 atcttcgggt gacggtctcc cgattccttg ccgctgccgc cgagcgtcac gccgtggaga
     1201 agcgaagtat tgtcgcccac ggtcgcagtc tctcctatga cgacgcccgt ggcgtgatca
     1261 atcatgatgc cgatcccgat ccgcgcggcg ggatggatat ccacgccgaa aatgcgtgag
     1321 gactggctct ggaggtaaag cgcgaagtcg cgccgtcctt ctttccatag ctcgtgcgcg
     1381 aaccggtgag tgacgagggc atggaagcct ttgaagtaga gcagcggctc cagataccgg
     1441 ctgcatgcgg gatcccggtc gtagactgcc gcaagatcgg cgcggaacgc ctggcccagc
     1501 tcgggttgcc gggacagcac gtccagaaac gtctgtccga tcagcccagc atcgacatcg
     1561 gagtggttga gtcgctgggc aagccgatgg cagacggcgt tctcgagccg ctcatggctc
     1621 agcacggtcg caaagatgaa cccaccgagt gccggctcgg cgagcatggc ctcctcggct
     1681 tcacggcgca cctcggccca gatcggatcg acctgggcga tcttactctg ggcctggaac
     1741 cggggggtcg gtgccatgta cgatctcctg ccagggttga cgttggcttg ctggcccttt
     1801 ttcacccagc cctaacgagc gctcgtcccc tagtcaacgt cgagctgccg gactggatgt
     1861 tcaaacttac gatgcctgca aagcggcgcc ttgccggcta gttgaagaaa acacgtggga
     1921 tcccgtgccg ttgcggacaa gaccaaaact caacgcatgc aagacaggag caagaacccc
     1981 ggctcgccga cgcgctggcc aacagatagg tgacctgggg ccttagcccg cttgcccgtc
     2041 ctgcctcctt ttccactcgc cgtagagatc cgggcgcgac tccaggcgaa caggctcatg
     2101 ccggattttc attgactgct ccgagaacgg tttggcgaga tcggcggaaa tggctgccct
     2161 ggaaagcccg tagggctccg cgtcctcgct cgagtgggcg aaaacgacct cctccactcc
     2221 ggcaatacgc attgcagcca tgcacatggg gcatggctgg ccactcgcat atacagtgca
     2281 gccgctgagg tccggcgtgc caagtgcttg tgccgcggcg cgcactgcca tgatctcggc
     2341 gtgagccgtc ggatcgttag tgaccatgac ctcgttcacg cctgttgcga tgcgcttgcc
     2401 gtccttcaca acgaccgctc cgaacggtct gccacccttc tcgatattgt cgaaagcaag
     2461 ctcgatggct tggcggagat actgctcgct ggtgctcatc ttacggatcc ttcagctggc
     2521 ggctcagccg cggaacttag atgggttact ggccagagtg aaatttcaaa ggacgcgatc
     2581 gcaacccgcg ccccgtccta gccgacctcg aacaccacac ggcccctaac acgcccagcc
     2641 agaa
//
LOCUS       DASUMG010000342         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_45282, whole genome shotgun sequence.
ACCESSION   DASUMG010000342 DASUMG010000000
VERSION     DASUMG010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45282"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFX46_05610"
     CDS             complement(<1..698)
                     /locus_tag="VFX46_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEX5600089.1"
                     /translation="MLQRGYEFDQQRLLPTSVEDRLSSWVSRCVGVALLLLGVAAWLS
                     LVSWSVSDPSLTHTVAIGPTRNLLGTGGAIISDLMLQLLGLASAFLALAPAVWGFELL
                     SSGRLPFLRSRIVLFALSILGLAAGCSALPTAANWPLHNGFGGIIGDLLYNLSAGLVA
                     TAVPVRSGAVAGLLMFSGGLAAFTCSLGLSGRWASEFWQPEAAARRVTTPPVRFRPSL
                     QFPAIFRGRRQRVTR"
     gene            complement(733..1950)
                     /locus_tag="VFX46_05615"
     CDS             complement(733..1950)
                     /locus_tag="VFX46_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5600090.1"
                     /translation="MSTHTGLEALAQGAILSPFTRLARLLEGIPPGHEKVIELTVGDP
                     KERMPDFVIDRMAEAKELLGTYPKIRGSDELRKAIAGWIERRYDLAGRIDPAREIIPI
                     NGSREGLFYAMLPAVGRKRVQGRPVVLLPNPFYHTYLGAAYATNCDPYFLNATEATGH
                     LPDLGALERETDILERTVAFYLCSPANPQGAVASTSYTLKALELARRYDFMLFFDECY
                     SEIYTGEPPTGGLEVAAKTPEGFRNLVVFNSLSKRSNLPGLRSGFAAGDGEFLESFAE
                     VRNLVAPQMPGVVQHASAAVWSEEQHVATIRQSYRNKFDICDEILAGRFGYRRPEGGF
                     FLWLDVSQFGGGVETTVTLWQRAGVKVVPGAFLAQPGPDGVNPGDDRIRVALVNDPAT
                     IREALERIVSVLS"
     gene            complement(2022..2882)
                     /locus_tag="VFX46_05620"
     CDS             complement(2022..2882)
                     /locus_tag="VFX46_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600091.1"
                     /translation="MAQSSAAKVFERVDAAGPDAPLAERSSGLAAPASKSDPWKRFTV
                     DVWITDLNSVEFGIYRHSRNRAKSRQFSTDMDIFAEVEEDGRRTGLIGYREDLWKQRS
                     GMEKRLIVRLFSDTLNWRATMDLMIGRSLQLTLGARGIPVLAYAVNTHEDKSIVYVER
                     SANKWPILPENFSFFIFRDGKPVFYRLRHDLISVGGDYTLFDERDRKVGHIDGRLFSF
                     GYWTCKVRAEHADDKLMTVMKLFTATIGFNKACRRHIARLAADVRAGRVEPKLERYEA
                     EYYLNPRRVR"
     gene            complement(3021..3683)
                     /locus_tag="VFX46_05625"
     CDS             complement(3021..3683)
                     /locus_tag="VFX46_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX5600092.1"
                     /translation="MTAAVERLSDIRRRIAEAARQAGRDPESVHLIAVSKTFDAPQVL
                     PVIEAGQRLFGENRVQEAKAKWPPLKAQFPDLELHLVGALQSNKAKEAVQLFDAIHSI
                     DRPKIARVVADEMAKQGRKLKLFIQVNTGEEPQKAGVMPGELESLLRYCQDELRIDIA
                     GLMCIPPVDEEPAVHFAFLAKRAKELGLKELSMGMSADFETAIAFGANYVRIGSAIFG
                     ER"
     gene            3909..>4834
                     /locus_tag="VFX46_05630"
     CDS             3909..>4834
                     /locus_tag="VFX46_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX5600093.1"
                     /translation="MAIERYNAPAREKHWQQVWESRDVFKVSNEDKRPKCYVLEMFPY
                     PSGRIHMGHVRNYTMGDVFARYKRAKGFNVLHPMGWDAFGMPAENAAMERKVHPAKWT
                     YDNIATMRSQLKSMGLSIDWSREFATCDVDYYHQQQKLFLDFYKKGLAYRGVGKVNWD
                     PVDQTVLANEQVIDGRGWRSGALVETREMPVWYFKITAYADELLEALNELTRWPEKVR
                     VMQANWIGRSEGLLMKYALAGDKLPAGWKELEVFTTRADTIFGGSFCALSPDHPLTKE
                     LAKSNPDLAAFVEECRRVGTSEEEIIRVDKKG"
BASE COUNT         1017 a         1485 c         1433 g          899 t
ORIGIN      
        1 cgcgtcacgc gctgccggcg tccacggaag atggctggaa actgaaggct tggacggaac
       61 ctgacggggg gcgttgtcac ccgccgcgcg gcagcttccg gctgccaaaa ctcggatgcc
      121 caacgtccgc tcagccccag agagcaggtg aacgccgcga gcccgccgga aaacattagg
      181 agccccgcca ccgctcccga gcggacgggc accgccgtcg cgaccaatcc agctgacagg
      241 ttgtagagta aatcgccgat gatgccacca aacccgttat gcagcggcca gtttgcggca
      301 gtaggaagcg cggagcagcc agcggccaac ccaaggattg acagggcaaa caggacaatc
      361 cgcgaccgga ggaatggcag gcggccactc gaaagcagct caaagcccca taccgccggc
      421 gccagcgcca gaaacgccga tgccaggccc agcaactgca gcatcagatc agagatgatg
      481 gcaccaccag tcccaagaag atttcgcgtg ggaccgatgg caaccgtatg cgtcaagctt
      541 ggatcactga ccgaccagga gaccaagctg agccaagcgg cgacaccgag gagaaggagc
      601 gcgacaccga cacagcggga aacccagctc gaaagcctgt cctccaccga agtggggagt
      661 aaccgctgct gatcgaactc gtaaccccgc tgcaacatca cgccacttgg atctacgaca
      721 caagccgaga aattaggaca gcaccgatac gatgcgttcg agcgcttccc tgatcgtggc
      781 cgggtcgttc accaacgcga cgcggatgcg atcgtcccct ggattcacac catcgggacc
      841 cggttgcgcg aggaaagcac caggcacaac tttgacaccc gcgcgttgcc aaagagttac
      901 cgtagtttca acgcctcccc caaattggct cacatcaagc cataggaaga agccgccctc
      961 tggcctccgg taaccaaagc gacccgccag gatctcatcg caaatgtcga acttgtttcg
     1021 ataggactgg cggattgtgg caacatgctg ctcctcggac cagacggccg ccgaggcatg
     1081 ctgcacgaca cccggcattt gcggcgcgac aaggtttcga acctcggcaa agctttcgag
     1141 aaattcgcca tcgccagcag cgaagccgga gcgcaatccg ggcaaattgg accgctttga
     1201 caaggagttg aatacaacga gattgcgaaa gccttccggc gtcttggccg caacttcgag
     1261 gccgccggtc ggcggctcac ctgtgtagat ctccgagtag cactcgtcga agaacagcat
     1321 gaagtcgtag cggcgagcca actccagcgc tttcagtgtg tatgaggtgc tcgcgactgc
     1381 accctgcgga ttggccgggg agcagaggta aaaggcgacc gtccgctcca ggatgtccgt
     1441 ttcgcgttcc agcgcgccga gatctggcaa gtgtccagtc gcctcggtcg cattgaggaa
     1501 atacggatca caattggttg catatgccgc gccgagatag gtgtgataga aggggttcgg
     1561 aagaaggact acgggtcggc cctgaaccct cttgcggcca acagctggca acatcgcgta
     1621 gaacaggccc tcgcgtgagc cgttgatcgg aatgatttcg cgagcgggat cgatccggcc
     1681 agcaaggtcg tagcggcgct ctatccaacc ggcaatggct ttgcgcagct catccgagcc
     1741 gcggatcttc ggataggtgc ccaagagctc cttcgcctcg gccattcggt cgatgacgaa
     1801 atcgggcatc ctttccttcg gatcgccgac ggttagctca atgaccttct cgtgccctgg
     1861 agggatacct tcgagcagtc gagcaagccg cgtgaacggc gacagaattg cgccctgcgc
     1921 cagggcctca aggccggtat gtgtactcat aaggactcgt cctcgctaga gtgtgggcga
     1981 ggtctaccga acgttcgcaa tcggcgccag tcccagccgc ttcatctgac ccgccgtggg
     2041 ttaaggtagt attccgcttc gtagcgctcg agctttggct ctacccggcc tgcacgcacg
     2101 tcagcggcca atcgcgcaat atgccggcgg catgctttgt tgaatccgat tgttgcggtg
     2161 aaaagcttca tgacggtcat cagcttgtcg tcggcatgtt cggcgcggac cttgcaggtc
     2221 cagtaaccga aagaaaacag acgaccatcg atgtgaccaa ccttgcggtc gcgctcgtcg
     2281 aagagcgtgt aatcgccgcc gacgctgatc aggtcgtggc gcagtcgata gaataccggc
     2341 ttgccgtccc tgaatatgaa gaatgagaaa ttttccggca aaatcggcca cttgttcgcc
     2401 gagcgctcca cataaacgat ggacttgtcc tcgtgcgtgt tgacggcata ggccaagacc
     2461 gggatgccgc gcgccccgag cgtaagctgc aggctccggc cgatcatcag gtccatggta
     2521 gcgcgccagt tgagcgtgtc cgagaacagc ctgacgatca gccgcttttc catgccggac
     2581 cgctgcttcc agagatcttc gcgatagccg atgagaccag tgcggcggcc gtcctcttcg
     2641 acctccgcga agatgtccat gtccgttgag aactggcggg atttggcgcg gttgcgcgaa
     2701 tggcgataga taccgaattc gaccgagttg aggtcggtga tccagacatc gactgtaaac
     2761 cgcttccacg ggtcagattt ggaggcgggg gctgccagac ctgacgacct ttccgctagt
     2821 ggagcatccg gccccgctgc atccacccgc tcgaagacct tagccgctga gctctgtgcc
     2881 atgcttcttc cccgcctcgc agcagatgcg aatcagtttc ttgcgacctt agcaccgcaa
     2941 ggcgggatga tccgggtgat cacagaattg gatctggaaa acttcagttc gggaggcgct
     3001 gaattggctt ccgtcacgcc ttaccgctcc ccgaaaatag cgctcccaat tcgcacgtag
     3061 ttcgccccaa aggcaatggc cgtttcgaag tcggcgctca tgcccatgct gagctcctta
     3121 aggccaagct ctttggcgcg tttcgccagg aacgcgaaat gcacagcagg ctcctcatca
     3181 acgggcggaa tgcacatcag gccggcgatg tcgatgcgca actcatcttg gcaataccgg
     3241 agcaggcttt ccagctcgcc gggcatcaca cccgccttct ggggctcctc gcccgtattc
     3301 acttgtatga agagcttgag cttccggccc tgcttggcca tttcgtcggc gaccacccgc
     3361 gcgatcttcg ggcgatcgat cgaatgaatg gcatcaaaga gctgcacggc ctctttggct
     3421 ttattcgact gcagcgcgcc cacgaggtga agctcgagat cggggaactg cgccttgagc
     3481 ggcggccact tggccttggc ctcctggacc ctgttttccc cgaacagccg ctgccctgcc
     3541 tcgatcaccg gcaggacttg cggcgcatcg aaggtcttgg agacggcgat cagatgaacg
     3601 gattcagggt ctcggcctgc ctgacgtgca gcctccgcaa tgcgtcttcg aatgtctgag
     3661 agccgttcga cagccgcagt catcgagttc acccgtccaa tcctcaagat cgccagcgca
     3721 gacggccggt atgcaatgga tttcgccaca cgtcgataga tagggacgat ctgccgattg
     3781 catcgggctg agcgacgccc tattccctcg gtaaaatcgg gcggaccggc ggttcagctc
     3841 cggcttgacc gtcctttacc tttcaggctg tatcgcccgc gatcgtcact cgaaagcaca
     3901 gtagccccat ggccatcgaa cgctacaacg cgcctgcccg agagaagcac tggcagcaag
     3961 tttgggaaag ccgcgacgtc ttcaaggttt cgaacgagga caagcggccg aagtgctatg
     4021 tgctcgagat gttcccctat ccctctggcc gcatccacat gggccatgtg cgcaactaca
     4081 cgatggggga tgtcttcgcc cgctacaagc gcgccaaagg cttcaacgtc ctgcatccga
     4141 tggggtggga cgccttcggc atgccggccg agaacgcggc tatggagcgc aaggtccacc
     4201 ctgcgaagtg gacctacgac aacatcgcca ccatgcgctc ccagctcaag tcgatgggcc
     4261 tgtcgatcga ctggtcgcga gaattcgcaa cttgcgatgt cgattattac caccagcagc
     4321 agaagctgtt tctcgacttc tataagaagg ggctcgctta ccgaggcgtc ggcaaggtca
     4381 actgggaccc cgtcgaccag acggttctcg ccaacgaaca ggtgatcgat ggccggggtt
     4441 ggcgctccgg ggcgctcgtc gagacccggg agatgccggt ctggtatttc aagatcaccg
     4501 cctacgccga cgagctgctg gaggccttga acgagctgac gcgctggccc gagaaggtcc
     4561 gcgtcatgca ggccaactgg atcggccgct ccgaaggctt gctgatgaag tatgccttgg
     4621 cgggcgacaa gcttccggcc ggctggaaag aactcgaggt cttcaccacc cgggccgaca
     4681 cgatcttcgg tggcagcttc tgcgctttgt ctcctgatca cccgctgacc aaggagctcg
     4741 ccaagtcgaa tcctgacctg gcggccttcg tggaggagtg ccgccgtgtc ggtacgtccg
     4801 aggaggaaat tattcgggtc gataagaagg gatt
//
LOCUS       DASUMG010000343         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_527354, whole genome shotgun sequence.
ACCESSION   DASUMG010000343 DASUMG010000000
VERSION     DASUMG010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527354"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFX46_05635"
     CDS             complement(<1..1151)
                     /locus_tag="VFX46_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX5600094.1"
                     /translation="MRGLNGAGKDRTLLTGAKPEVPRRSLRPLLGLKPYVLKHPGMLV
                     LAGVALLIAALAMLTLPLAVRRVIDFGFSGSDAAFINRYFSMLMLIGLVLAVASAARF
                     YFVNWIGERVVADLRADVFRHLSQLGPDFYETTHSGEVTSRLTADTTQIKAAAGTAIS
                     QALRNIIMLFGALAMMIWTSPGLSFLVLLAIPVIVLPLIAYGRLVRRRSRRAQDTLAD
                     ASALAVENLAAVRTMQAFTNERFVSDRFAKAVERAFDAARAGLQARAGLTAMTIFLVF
                     ASIVGVLWYGAEAVVRGEITGGRLSQFVLYAVFAAAALAQLSEVWGEVSQAAGAAERL
                     AELLAVKPQIRTPDRPVPVPEPARGEISFRHVSFRYPSREESALSDISF"
     gene            1356..1574
                     /gene="rpmE"
                     /locus_tag="VFX46_05640"
     CDS             1356..1574
                     /gene="rpmE"
                     /locus_tag="VFX46_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX5600095.1"
                     /translation="MKKDIHPDYHPIKVVMTDGTEFVTYSTYGKEGDTLTLDIDPKTH
                     PAWTGGDQKLVDRGGRLSRFKKKFEGLF"
     gene            complement(1658..2200)
                     /locus_tag="VFX46_05645"
     CDS             complement(1658..2200)
                     /locus_tag="VFX46_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEX5600096.1"
                     /translation="MGSTSRCSEVSGVTVSFGEHFAASEQFDAIYAQGMALVERTAAY
                     LDGPGRQEAKQLKPHVALTYSTESMRLTARLLDLASWLLIRRSLRDGDITEEEARRKR
                     ARLKLHTTGRPSHIPNFDELPQQLQALIKESFELSDRILRIDRSLEAVSGNEASRPAS
                     KNPVAAQIAAIQAAFNAAIR"
     gene            complement(2506..2694)
                     /locus_tag="VFX46_05650"
     CDS             complement(2506..2694)
                     /locus_tag="VFX46_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEX5600097.1"
                     /translation="MDWDEVRPKAHSIITVGEPLHELSVSDLEERLAALREEIRRVEK
                     EIEAKRAHEKAASALFKR"
     gene            complement(2839..>3142)
                     /locus_tag="VFX46_05655"
     CDS             complement(2839..>3142)
                     /locus_tag="VFX46_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX5600098.1"
                     /translation="AEYERLGGSVIYAGKPYRPIYDLAFERLEDLRGGPVARERILAI
                     GDGVKTDIRGAANAGIDSVFIASAIHVTGDLDAAAIEQLFKECDRHPVAALPALVW"
BASE COUNT          638 a          988 c          948 g          568 t
ORIGIN      
        1 tcgaaggaga tgtcggacag ggccgactcc tctcgggaag gatagcggaa gctgacgtga
       61 cgaaatgaga tttcgcctcg ggcgggctcc gggactggta ccggccgatc cggcgtgcgg
      121 atctgaggct tcaccgcaag gagctccgcg agccgttcgg cggctcctgc ggcctgactg
      181 acctcacccc acacctccga gagctgggca agcgcagcgg ccgcgaagac cgcgtaaagg
      241 acgaactggc tgagccggcc gcccgtgatc tcgccgcgaa cgacggcctc cgccccatac
      301 cagagcacac ccacgatgct ggcgaagacg aggaatatcg tcatcgccgt aagtccggcc
      361 ctggcctgaa gccccgcccg ggcggcatcg aaggccctct cgaccgcttt ggcgaaccga
      421 tcggacacga agcgctcatt cgtgaaggct tgcatcgtcc gcacggcggc taggttttct
      481 accgcgaggg cagacgcatc tgccaacgtg tcctgggccc gccgggagcg gcggcgtacg
      541 agccgcccgt atgcgatgag cggcagcacg atgacgggga tcgcgaggag aaccagaaat
      601 gacaggccgg ggctcgtcca gatcatcatt gcgagcgccc cgaacagcat gattatgttg
      661 cgcagggctt ggctgatcgc ggttcccgcc gctgccttga tctgcgtggt gtcggctgta
      721 agtcggctcg tcacctcacc tgagtgagtg gtttcgtaga aatcgggccc cagctgtgac
      781 agatgccgga acacgtcggc gcgcaaatcc gccaccacgc gttcgccgat ccagttcaca
      841 aaataaaaac gggccgcact ggcgacggcc agcaccagcc caatgagcat cagcatcgag
      901 aagtagcggt tgatgaacgc cgcgtcggag ccggaaaacc cgaagtcaat gacccgccgc
      961 accgcaagcg gcaatgtcag catggccagc gccgcgatga gcagcgccac gccagccaga
     1021 acaagcatgc ctgggtgctt caacacatag ggcttgagcc ccagaagagg acgcagggag
     1081 cggcggggta cctccggctt cgctccggtc aacaaggtcc ggtccttgcc cgcgccgttc
     1141 agccctctca ccgcatttcc tacctgactg gatgatcccg gttctgtctt acgtcagaaa
     1201 gcgctgcctt ggcggcctgt cccgatgccg catcggccag catctcttgt gccttgcaga
     1261 gctttcaact atgtaggccc aacgcatccc tgaaatgaac gtaatctgtc cgctcaagtg
     1321 agcgagcggg ccgtgtgttg ccgagagctg ccaagatgaa aaaggacatc catcccgact
     1381 accacccgat caaggtcgtg atgaccgacg ggaccgagtt cgtgacctac tcgacctatg
     1441 ggaaagaggg cgacacgctg acgctcgaca tcgatcccaa gacgcacccg gcttggacgg
     1501 gcggcgatca gaagctcgtc gaccggggcg gccgtttgtc ccgcttcaag aagaagttcg
     1561 aaggcctgtt ctaatcggga ataagccggc ttcaattggt ttggatgcaa ccgggccgcc
     1621 cgcccggttg catcattctg tgcatggctg ctagaggtca gcgaatggcg gcgttgaagg
     1681 cagcctgtat cgcagcaatt tgagcggcaa cgggattctt gctagccggc cgacttgctt
     1741 cgttccccga tacggcttcg agcgatctgt cgatccgcag gatacggtcg cttagctcga
     1801 agctttcttt gataagagcc tgcaactgct gcggtagctc gtcgaaattt gggatgtggc
     1861 tgggccggcc ggtcgtgtgc agcttgagac gcgcgcgctt gcgccgggct tcctcctcgg
     1921 tgatgtcgcc gtcgcgcagc gagcgacgga tcaggagcca ggatgctaga tcgagaagcc
     1981 gcgccgtaag ccgcatgctc tccgtggaat aagtgagcgc gacgtgcggt ttcagctgct
     2041 tcgcctcctg gcggccaggg ccatcaaggt aggctgcggt gcgctcgacg agtgccatgc
     2101 cctgcgcgta aattgcatca aactgctctg acgcggcgaa gtgctcaccg aaggagacgg
     2161 tgacgccaga aacttcggag caacgcgagg tgctacccat gatctactca cgccacaagt
     2221 gtaccaacca caagacggaa cattcttttg cgtgaacgtc aatcatttct gcctcattgg
     2281 gcacaattct aggcagcaac ccggttaccc tctggaaaaa tgagccgccc gaaggcggct
     2341 ccagagtgac agggaggcgt caaacagagt gggtgagcca ctcggcatcc agaaaactgg
     2401 ataagctgca ctctaagcga acgatcctaa cggaaggtaa atccgtggct caaagcggca
     2461 cagaattgcg gtatccgccc cataaagctg ccgagcccgt aagctctacc gcttaaacaa
     2521 tgccgacgcc gccttctcgt gcgcccgctt cgcctcgatt tctttttcga cacgccgaat
     2581 ttcctcgcgc agcgcagcga ggcgctcttc caggtcgctt accgacaact cgtgcagcgg
     2641 ctccccgacc gtaatgatac tgtgagcttt cggtcgaact tcatcccagt ccatggccaa
     2701 ctcccgctgt ggcgccgcga tgctcgcaac ttacggctca gacctcctcc cgagcctgcc
     2761 ccaagaatgg tcacctggag cctctagtgc aagggcgccc tcttttccgg aaaagttgcg
     2821 aagtttgcca gctccggcct accaaaccaa cgctggcagc gccgccaccg gatggcgatc
     2881 gcactccttg aagagctgct cgatggcagc ggcatcgagg tcgcccgtga cgtggatcgc
     2941 gctggcgatg aacaccgaat cgatgccggc gttagcggcg ccgcggatgt cggtcttcac
     3001 gccatcgcca atggccagga tgcgctcacg ggccaccggg ccacccctca agtcttcaag
     3061 gcgctcgaag gcaaggtcat agatcggccg gtatggtttt cctgcgtaga tcacgctgcc
     3121 gccgaggcgc tcgtattcgg cc
//
LOCUS       DASUMG010000344         2710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_206466, whole genome shotgun sequence.
ACCESSION   DASUMG010000344 DASUMG010000000
VERSION     DASUMG010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206466"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..753
                     /locus_tag="VFX46_05660"
     CDS             133..753
                     /locus_tag="VFX46_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600099.1"
                     /translation="MTNEPGRPEQGSTGGNVGEVKERLEREAEAARESFQRVGEKVQR
                     QASDVGSDLQAQAQKMVSEQKEMARSGLMDMVSAIRTAADDLERHEQSQFANLARGLA
                     RGIEDFSQSIGRRNFQEMLTDVQRFARDHPAAFFGGAVLAGLAIARFAKSSSEHRHEA
                     RFGRQAEQTSGARYPMQRAGQGGQLADFARQGREDVGGVQTGNLTR"
     gene            759..1217
                     /locus_tag="VFX46_05665"
     CDS             759..1217
                     /locus_tag="VFX46_05665"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX5600100.1"
                     /translation="MAEVRHTETAAPEAPLSHLTHGAGEARATHSLADLVAELGRDVS
                     NLVSKEIELLRAEVSQSYSNVQTAGGSLAAAAICLLAALLILLQALIVALTNLGMGAG
                     WASLLVGVVVGIVGLVLLQNGRSLLKEGIVPKRTQAQVQRDAEMLKEQVK"
     gene            1214..2266
                     /locus_tag="VFX46_05670"
     CDS             1214..2266
                     /locus_tag="VFX46_05670"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEX5600101.1"
                     /translation="MTETPSHAEREVERARADLSQTLDALKERLTFGRIVDEARTQFF
                     GDGGSEFVTNLGRQIRENPLPVALVGLGALWLMLGERRGPSRTVRVYPSEAERMGGYR
                     QGFGERAQQAAAQMGTGLRSTAETMRETREGMTERAREVAAEAGESMRSAAGAVSETV
                     RHAGETAADWMHQAGSAARTAGGSMGELGQRTSESIRTMAEEQPLVLGAIGMALGALV
                     GAMLPSTRMENQYLGEARDQLREAIVEQGGELYERGKVTATEAYRAAAEEARKQGLLP
                     EEGEGKTFAEKAEQVLTKAGEAAKEAGQREAGAALTEGGPKAQTAAGEGVQPQESIRP
                     GSPVIPDQPSAPVRRS"
     gene            complement(2348..>2710)
                     /locus_tag="VFX46_05675"
     CDS             complement(2348..>2710)
                     /locus_tag="VFX46_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600102.1"
                     /translation="PEALAGWSAEDVETQDAAAAMFGGIRAARTYEKGGVEVKVQIMG
                     DSPLLATWMPMMSNSAVAAAMGKMTRIGKRRALQTKEGQVIMVVGNRFLVMIEGSASM
                     EDKTAYAAAIDFDTLETF"
BASE COUNT          552 a          816 c          898 g          444 t
ORIGIN      
        1 ctcgttgaaa gcgccacacg accggagcct ggcgcaccga agctacacaa ccgtgccctt
       61 ggctcctcgc gactaccttg ccctgcgagg caaagcccgc ggcgcataag ccatcggatg
      121 gagtatcggc caatgacgaa cgaacccggc agacctgagc aagggtcgac aggcggcaat
      181 gtgggcgagg tgaaggagcg gctcgagcgc gaggcggagg cggcgcgcga gtcttttcag
      241 cgggtcggcg agaaggtgca gcgccaagcc agcgacgtcg gttccgatct gcaggcccaa
      301 gcacaaaaga tggtctccga gcagaaggaa atggcgagat ccggcctgat ggacatggtt
      361 tcagccattc ggaccgctgc cgacgacctt gagcgccacg aacaatcgca attcgccaac
      421 ctcgcacgcg gcttagcgcg cgggatcgag gacttctcgc aatcgatcgg tcgccgaaac
      481 ttccaggaga tgctgacgga cgtgcaacgg tttgcgcgtg atcatccggc agcctttttc
      541 gggggtgcgg tgctggccgg acttgccatc gcccgcttcg ccaagagctc gagcgagcac
      601 cggcatgaag cccgctttgg tcgccaggcg gagcagacgt caggcgcgcg ataccccatg
      661 caacgcgccg gacaaggtgg tcagctcgcg gatttcgcgc gtcaagggcg tgaagacgtc
      721 ggtggcgtcc aaaccggcaa tctcacgagg tgaacgccat ggcagaagtc agacatacgg
      781 aaaccgcggc ccccgaggct ccgctttcgc acctgacgca cggagctggc gaagctcgcg
      841 ccacgcactc actggccgat cttgtcgctg agctggggcg ggacgtctcc aatctcgtca
      901 gcaaggagat cgagctactg cgagccgagg tgagccagag ctactccaat gtgcagacag
      961 cgggcggcag tcttgccgcc gcagccattt gcttgctggc tgctctactc attcttttgc
     1021 aggcgctcat cgtcgccctg accaatttgg gcatgggtgc cggatgggcc tcgctgctgg
     1081 tcggcgtggt tgtcgggatc gtcggcctcg tcctgctcca gaacggccgt tccctactca
     1141 aagagggcat cgtgccaaaa cgcacgcagg cacaagttca gcgcgatgct gagatgttga
     1201 aggagcaagt caagtgaccg agaccccaag ccatgcagaa cgagaggtcg agcgcgcccg
     1261 agccgatctc agccagacgt tggacgccct gaaggagcgg ctgacgttcg gccggatcgt
     1321 cgatgaggcg cggacccaat tctttggaga cggcggcagc gagttcgtga cgaacctggg
     1381 ccgccaaatt cgcgagaacc cgttgccagt cgccttggtc ggccttggag ccctctggtt
     1441 gatgcttggg gagcgcagag ggccgtcgcg caccgtccgg gtctatccat ccgaggcgga
     1501 gagaatggga ggctaccgac aaggcttcgg cgagcgcgct caacaagctg cagcgcaaat
     1561 gggaactggg ctgcgctcta ccgcagaaac catgcgggaa acccgcgagg gtatgaccga
     1621 acgggcccgc gaagtcgcgg cggaagcagg cgagtccatg cgttcagcgg ccggtgcggt
     1681 gagcgagacg gtgcgtcatg ccggcgaaac cgccgcggat tggatgcacc aagccgggag
     1741 cgcggctcgg actgcgggcg gcagcatggg cgagctcggt cagcggacta gtgagagcat
     1801 tcggacaatg gcggaggaac agccgctcgt ccttggagcc attggcatgg cgctcggtgc
     1861 cttggtcggc gcgatgcttc cgagtacacg gatggaaaac cagtacttgg gcgaggcgcg
     1921 agatcagctc cgagaagcaa tcgtcgaaca aggcggcgaa ctctacgagc gaggaaaagt
     1981 aaccgctacc gaggcttatc gtgcggctgc ggaggaggcc cggaaacaag gtctgcttcc
     2041 cgaagagggc gaaggcaaga ctttcgccga aaaggccgag caggttctaa ccaaggccgg
     2101 tgaagcggca aaggaggctg gccagcgcga ggcgggcgca gcgctgacgg agggcggacc
     2161 gaaagcgcag acagcggcag gtgaaggtgt ccagcctcaa gaaagcataa ggccgggctc
     2221 acctgtgata cccgatcagc cgtcggcgcc tgtcaggagg agctgagcaa gtgagacgaa
     2281 ccggcatgca gttgcgcttg catgccggat tttcgatcgc acgctattct tgagcccacc
     2341 tgggctttca gaacgtctcg agcgtgtcga agtcgatcgc cgccgcgtaa gccgtcttgt
     2401 cctccatcga ggcagagccc tcaatcatca ctagaaatcg attgccgacc accatgatga
     2461 cctgaccttc cttggtctgc agggcgcggc gtttgccgat cctcgtcatt ttgcccatgg
     2521 ctgcagcgac ggctgagttg ctcatcatcg gcatccaggt cgcgagcaag ggcgagtctc
     2581 ccatgatctg cactttgact tcgacaccgc ccttttcata agtccgcgcg gcccggatac
     2641 ctccaaacat cgccgcggca gcatcctggg tctcaacgtc ttcagcgctc cagcccgcga
     2701 gtgcctccgg
//
LOCUS       DASUMG010000345         4475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_929068, whole genome shotgun sequence.
ACCESSION   DASUMG010000345 DASUMG010000000
VERSION     DASUMG010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929068"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VFX46_05680"
     CDS             complement(<1..481)
                     /locus_tag="VFX46_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600103.1"
                     /translation="MPDSKRQDLDVGTGANRRRIAVLRDEGRAPCLLWLPGFKSDMIS
                     TKATALSEWARGKGLACTRFDYSGHGQSEGRFEDGTLSRWLEETRAVFTQRTEGPVIV
                     LGSSMGGYIALLLLRQLIAEAPEEAARIKALVLIAPAWDMTEELMWKEFPNEIRQEIM
                     "
     gene            516..1748
                     /locus_tag="VFX46_05685"
     CDS             516..1748
                     /locus_tag="VFX46_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5600104.1"
                     /translation="MPNHRPTILQIIPRLDTGGAERTTIEMTQAITAAGGRALVVTEG
                     GRMVPQVTEAGGEVMTFNAGTKNPLSMLINARRLQKLVEREQVDLLHARSRAPAWTAL
                     WAARLSGRPFVTTYHGAYNEKGALKRTYNSVMARGDVVIANSHYTSRLIQSRYGTPES
                     RIRVIHRGIDEESFDPSNVSPERVERLQRHWGIRPGQRVVLHAARLTGWKGQSVVIAA
                     AARLAQQNFAGDDWVVILAGDAQGRTDYVRDLEQQIAASGLSQRVRLVGHVEDMPAAY
                     VTAHVAVVASTEPEAFGRAAAEAEAMCCPVIATDIGATPETVRAEPVVSREEITGWLV
                     PPGDPEALAQRLREALSLSEEERAAIGKRARDHVCRSFSLGAMQAATLQVYDSLLGTR
                     LAEAWSAVSSSRTCVPSA"
     gene            1877..2407
                     /gene="infC"
                     /locus_tag="VFX46_05690"
     CDS             1877..2407
                     /gene="infC"
                     /locus_tag="VFX46_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX5600105.1"
                     /translation="MLRGTPDREPSGPRVNEAIRAREVRLIDENGRNLGIVSRAEALE
                     RAEAAGLDLVEVSPDSTPPVCKILDYGKFKYQEQKKAAEARKHQKTVEVKEIKMRPSI
                     DDHDYQVKMRAIRRFFDEGDKVKITLRFRGREMAHQDLGMDLLQRVKADLGDLAKVEA
                     EPRLEGRQMVMVLAPR"
     gene            2616..2816
                     /gene="rpmI"
                     /locus_tag="VFX46_05695"
     CDS             2616..2816
                     /gene="rpmI"
                     /locus_tag="VFX46_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX5600106.1"
                     /translation="MPKLKTKSSAKKRFKMTATGKVKVQAQGKRHGMIKRSPKFIRNA
                     RGTMVLCDSDAKIVKKYMPYDR"
     gene            2876..3256
                     /gene="rplT"
                     /locus_tag="VFX46_05700"
     CDS             2876..3256
                     /gene="rplT"
                     /locus_tag="VFX46_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX5600107.1"
                     /translation="MARVKRGVTAHARHKKVLKAAKGYYGRRKNTIRVAKQAVERAQQ
                     YAYRDRKRRKRNFRALWIQRINAAARQLGTTYGQFINGLNKAGIAVDRKVLADLAVHD
                     SAAFKALVEQANAAVQAARQAASA"
     gene            3327..4415
                     /gene="pheS"
                     /locus_tag="VFX46_05705"
     CDS             3327..4415
                     /gene="pheS"
                     /locus_tag="VFX46_05705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX5600108.1"
                     /translation="MAKFPELETLKSELLKQIEGAKDLAALEQVRIAALGKKGKIPEQ
                     MAKLGTLPPEQRREFGQATNTLKGAVTEALEARKAVLEAAALEERLATERADITLPVR
                     LGPLAEGRIHPVSQVFDEVIEIFADMGFSVAEGPDIETDDYNFTKLNIPEEHPARQEH
                     DTFYLRETTAADGSNRPRMLLRTHTSPVQIRTMLSQKPPIRIIAPGRVYRCDSDQTHT
                     PMFHQIEGLVIDESTHLGHLKWCLEEFCKAFFEVDEVKMRFRASHFPFTEPSMEVDID
                     AAAIGKPGHWLEILGSGMVHPNVIRNCGLDPEKYQGFAFGMGLDRIAMLKYGIPDLRD
                     MFAADLRWMKHYGFLPIDVPTLAGGLSP"
BASE COUNT          898 a         1410 c         1414 g          753 t
ORIGIN      
        1 ccatgatctc ctgccggatc tcgttcggga actccttcca catcagctcc tcggtcatgt
       61 cccaggcggg ggcgatgaga accaacgcct tgatccgagc agcttcctcg ggtgcctcgg
      121 ctataagctg ccggagcagt aagagggcaa tgtagcctcc catgctcgag ccgaggacga
      181 tcaccggtcc ctcggtcctt tgggtgaaaa cagcgcgtgt ttcctccagc cagcggctca
      241 gggtgccgtc ctcgaaccgc ccctccgact gaccgtggcc gctgtagtcg aaacgtgtgc
      301 aggcgagccc cttcccgcgc gcccactcgc tcagggctgt tgccttggtg ctgatcatgt
      361 cggacttgaa gcccggcaac caaagcaaac acggcgcacg cccttcgtcc cgcagaacgg
      421 cgatgcggcg gcgatttgcg ccagtaccaa cgtcgagatc ttgcctcttg gagtcgggca
      481 tcttactgag gtccttcctg ttagaggggc cgctcgtgcc gaaccatcgc ccgaccatac
      541 ttcagatcat cccgcgtctc gacaccggcg gcgccgagcg caccacgatc gagatgacgc
      601 aggccattac cgctgcaggc ggccgagcgc tggtcgtcac cgaaggcggg cgcatggtgc
      661 cccaggtgac tgaggccggg ggcgaggtca tgaccttcaa cgcgggcacg aagaatccgt
      721 tgtctatgct catcaatgcg cgccggttgc agaaattggt cgaacgtgag caggtcgatc
      781 ttctccatgc ccgcagtcgt gctcctgcct ggacggctct atgggccgca cgtctaagtg
      841 gcaggccctt cgtgacgaca tatcacgggg cctataacga gaagggcgcg ctcaagcgca
      901 cgtacaacag cgtgatggcg cgcggggacg tggtgatcgc caactcccac tacacctcgc
      961 gcctgatcca gtcgcgctac ggcaccccgg agagccgaat ccgcgtcatc caccgtggca
     1021 tcgacgaaga gagcttcgac cccagcaatg tttcacccga gagggtcgag aggctgcagc
     1081 ggcactgggg tattcggcca ggacagcgcg ttgtgcttca cgccgcgcgg ctgacaggct
     1141 ggaaggggca gagcgtggtc atcgcagctg cagcgcggct cgcgcaacag aatttcgccg
     1201 gtgacgattg ggtcgtaatt ctggctggcg atgcccaagg ccgaacggac tacgtccgcg
     1261 atttggagca gcaaatcgca gccagcgggc tttcccagcg cgtccgcctc gtcggtcatg
     1321 tcgaagatat gccggcggcc tatgtcacgg cccacgtggc cgtcgttgcc tcgaccgagc
     1381 ctgaagcgtt tggccgcgcg gctgccgaag ctgaggccat gtgctgtccg gtcatcgcaa
     1441 ccgacatcgg cgctaccccg gaaacggtgc gtgccgagcc cgtcgtgtca cgggaggaga
     1501 ttaccggctg gcttgtcccg ccgggcgatc cggaggcgct ggcgcaacgg ctccgcgaag
     1561 cgctctcgct ttctgaagaa gagcgtgccg ccatcggaaa acgggcccgc gatcatgtgt
     1621 gccgatcgtt ctcgctgggt gccatgcaag cggcgacttt acaggtctac gacagccttc
     1681 tcggcactcg actcgccgaa gcctggtcgg ccgttagctc atctcgcacc tgcgttcctt
     1741 ccgcttgact tggccgggga cattcccgat cttgctagtc caggtatcac ggtggccgca
     1801 acgagcgacc gccgcgttgc gtgtatgaga agcttggttt cgaaacaaca gagggagact
     1861 gaacatccgt cgaccaatgc ttaggggcac gccagaccgc gagcccagcg gcccgagggt
     1921 caatgaagcg atcagggcca gagaggtacg gctgatcgac gagaacgggc gaaatctggg
     1981 catcgtgagc cgcgcagaag ctttggagcg tgccgaagcc gcgggactcg acctcgttga
     2041 ggtgtccccc gactcaaccc cgccggtttg caaaatcctc gattacggaa aattcaagta
     2101 ccaagagcag aagaaggcgg ctgaggcgcg caagcaccag aagaccgtcg aggtcaaaga
     2161 gatcaagatg cggcccagca tcgatgatca cgattatcaa gtgaaaatgc gcgcgatcag
     2221 gcgcttcttc gacgagggag acaaggtgaa gatcaccttg cgtttccgcg ggcgcgaaat
     2281 ggcgcaccag gacttgggca tggaccttct gcagagggtc aaggcggatc ttggcgatct
     2341 ggccaaggtg gaagccgagc cccgcctcga aggccgccag atggtgatgg tcctggcgcc
     2401 gcgctaagcg ctggagctgc ttgcaccagc ggtatcttct gctttataag cgagccgagc
     2461 cgcccggcgg ggcatgccgt ggcggctcca ttacgtttgc acgaaaacac ggcggtcgca
     2521 tccttgctcc ctggcaggcg ccttgggggc gcggtcgtct cggttgcgat gcgagtgcag
     2581 cggcgccgcc acaaacaaca caaggagcaa gcaaaatgcc caaactgaag acgaagagca
     2641 gcgcgaaaaa gcgcttcaag atgaccgcca ccggcaaggt gaaggttcag gcgcagggca
     2701 agcgccacgg catgatcaag cggtccccga agttcatccg gaatgcgcgc ggcaccatgg
     2761 tgctgtgcga cagcgacgcg aagatcgtca agaagtacat gccgtacgac cggtgataag
     2821 cctgatcgga catcgaaacg atccgctcta atccttcatc tgacggagca cgacaatggc
     2881 acgtgttaag cgcggcgtaa ccgctcacgc gaggcacaag aaagttctca aggctgccaa
     2941 gggctactac ggccggcgca agaatacgat ccgcgttgcc aagcaggcgg tcgagcgggc
     3001 gcagcagtat gcctatcgcg atcgcaagcg ccgcaagcgc aatttccggg cactttggat
     3061 ccagcgcatc aatgccgctg cccgccagct cggcacaacg tacggccagt tcatcaacgg
     3121 gctgaacaag gccggcatcg cagtcgaccg taaggttctg gccgacctcg ccgtccacga
     3181 ttcggccgcc ttcaaggcgc tggttgagca ggcgaacgct gctgtgcagg ccgcccgcca
     3241 ggcagcatcg gcttaggctg gctagagggc gcgctaaggc gttcgctacc ggccagggta
     3301 atcggcgcgg ggcagaggca atcccgatgg cgaaattccc cgaactcgag acgctcaagt
     3361 ccgagctgct gaagcaaatc gaaggggcga aagatctcgc cgcactcgag caggtgcgca
     3421 tcgccgcgct cggcaagaag ggcaagattc cggagcagat ggcaaagctc ggcacgctgc
     3481 cgccggagca gcggcgcgag ttcgggcagg ccactaacac cctcaagggg gcggtcacgg
     3541 aagctctcga agcacgaaag gcggtcctcg aggccgcggc gctggaggaa cgcctcgcca
     3601 cggagcgtgc cgatatcacc ctgccggtca ggcttggtcc tctcgccgag gggcgcatcc
     3661 atccggtctc ccaggttttc gacgaggtca tcgagatctt tgccgacatg ggcttctcgg
     3721 tggccgaggg gccggacatc gagacggacg actacaactt caccaagctc aacattccgg
     3781 aagagcaccc ggcccgccag gagcacgaca ccttctatct gcgcgagacc acagcggcag
     3841 acggatcaaa ccggccgcgc atgctgctgc ggacgcacac aagcccggtg cagattcgca
     3901 ccatgctttc gcagaaaccg ccgattcgga tcatcgcccc tggccgcgtc taccgctgcg
     3961 acagcgacca gacgcacacg ccgatgttcc atcaaatcga gggcctcgtc atcgacgaaa
     4021 gcacccatct gggccatctc aaatggtgtc ttgaggaatt ctgcaaggcc ttcttcgagg
     4081 tggacgaggt gaagatgcgc ttccgggcct cgcacttccc gttcaccgag ccgtcgatgg
     4141 aggtggacat cgacgcggcg gcgatcggca agccggggca ctggctggag atcctcggct
     4201 ccggcatggt gcacccgaac gtgatccgca actgcgggct cgacccggag aagtaccaag
     4261 gctttgcctt cggcatgggc ctcgatcgca ttgccatgct gaagtacggc atcccggacc
     4321 tgcgcgacat gttcgccgcc gatctgcgct ggatgaagca ctacggcttc ctgcccatcg
     4381 acgtgccgac gctcgccggc gggctttcgc cctgagtcag tgtatccgcc gagcctctat
     4441 cgtacacagg gttagctgag ttgcgggacc accga
//
LOCUS       DASUMG010000346         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_446577, whole genome shotgun sequence.
ACCESSION   DASUMG010000346 DASUMG010000000
VERSION     DASUMG010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446577"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFX46_05710"
     CDS             complement(<1..185)
                     /locus_tag="VFX46_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HEX5600109.1"
                     /translation="MNALLTFSFLALAVAAGGVFWVSSELDKAGPLQEARSVVIRRGE
                     GARDIARRLEREGLISS"
     gene            complement(541..1806)
                     /gene="fabF"
                     /locus_tag="VFX46_05715"
     CDS             complement(541..1806)
                     /gene="fabF"
                     /locus_tag="VFX46_05715"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5600110.1"
                     /translation="MRRVVITGLGLVTPLGCGVEATWSRLLAGQSGARRVETFDVSDL
                     SCQIACIVPRGDGTDGTFNPNDWMEPKEQRKVDDFIIYAMAAADQALADAGYKASTTE
                     EQERAGVLIGSGIGGLQGIADTAILLQEKGPRRVSPFFIPGRLINLASGQISIRHQLK
                     GPNHAVVTACSTGAHAIGDAARLIALGDADVMVAGGTESPICRLAIAGFAACRALSTN
                     FNDQPERASRPYDKDRDGFVMGEGAGIVVLEELEHARARGARIYAEVIGYGLSGDAYH
                     ITAPAEDGDGAYRCMKAALQRAGIEPSDIDYVNAHGTSTPMGDEIELAAVERLFGSAA
                     EKLSMSSTKSAIGHLLGAAGAVEAIFSVLAIRDNVAPPTLNLDNPSVDSVIDLVPHKP
                     RKRQIDTVLSNSFGFGGTNASLILRRLDA"
     gene            complement(1908..2150)
                     /locus_tag="VFX46_05720"
     CDS             complement(1908..2150)
                     /locus_tag="VFX46_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX5600111.1"
                     /translation="MSDVAERVKKIVVEHLGVEAEKVTENASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAEHILTVGDAVKFLEKNANAA"
     gene            complement(2422..3159)
                     /gene="fabG"
                     /locus_tag="VFX46_05725"
     CDS             complement(2422..3159)
                     /gene="fabG"
                     /locus_tag="VFX46_05725"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX5600112.1"
                     /translation="MFNLTGRGALITGASGGIGSAIARKLHAAGASVAISGTKIDRLE
                     ALANELNERVHIVPCDLSDRAAVQKLAGEAEAKLGTLDILVNNAGVTKDNLFVRMKDE
                     EWDEVIAVNLTATFILCRAALRNMMRRQWGRIINITSISGIIGNPGQGNYAAAKAGMI
                     GMTKSLAREVASRGITANCIAPGFIKTAMTDVLTEKQVEAIAQHIPAAKFGEPDDIAA
                     AALYLASEEARYMTGQTLHVNGGMAMI"
     gene            complement(3166..>3630)
                     /locus_tag="VFX46_05730"
     CDS             complement(3166..>3630)
                     /locus_tag="VFX46_05730"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX5600113.1"
                     /translation="ANDNEPAQVVLSGHKSAIDRVAEVGRKHGVRRAIPLPVSAPFHC
                     SLMKPAADVMAEELGKVEIRRPVVPVVSNVLAEPISDPEEIRKRLVEQVTGTVRWRES
                     VLKMSELGVTDFYEIGAGKVLTGLIKRILKDANAVSVGSPADVDEALAALGR"
BASE COUNT          681 a         1193 c         1038 g          718 t
ORIGIN      
        1 tcgctcgaaa tgaggccttc ccgctcgagc cgccgggcga tatcgcgcgc gccttcgccc
       61 cggcggatga ccacgcttcg ggcctcctgc agcgggcctg ctttgtccaa ctccgacgag
      121 acccagaaca cgccgcccgc ggccactgca agcgcgagga agctgaaagt cagcaatgcg
      181 ttcacgaacc gcagcgttgg acccaacctt cttacctcgc ggcgtgttct cgaacccttc
      241 ggcctcgagg gtgcgcgtgt cggctcgagg gcctcagccg gactgcgcgg catgacgcct
      301 ggaggctgcg tccggtgcgt ccgtcgtcgt tcccttggcg gccgagcttt cagcatccgc
      361 tcgcagttcc tccaccaacc ctagcgcgac caaaggctgt ctcccgaatc acggatcagt
      421 ctaccgaatg gacgggctat tcagggctgg caagatgatc gaggaatgtg gcaacagcag
      481 gcagccgaca gcaagccccg tgtggctttg cggttactcc gccgcccgct gattggcgcc
      541 tcaagcatcg aggcgccgca gaattaatga agcgttggtt cctccgaagc cgaacgagtt
      601 cgacaaaacc gtgtcgatct gccgcttccg aggcttatgt ggaacgaggt cgattacgct
      661 atcgacggaa ggattgtcca ggttcaaagt cggtggagcc acgttgtctc gaatggcgag
      721 aacactgaaa atggcctcca ccgccccggc tgcaccaagg aggtgcccga tggcggattt
      781 cgtcgacgac atcgagagct tctccgccgc gctgccgaac aggcgttcga cggcggcgag
      841 ctcgatctcg tcgcccatgg gcgtcgaggt cccatgcgcg ttgacgtagt cgatgtcact
      901 cggctcgatg ccggcccgct ggagagcagc cttcatgcaa cggtaggcac cgtcgccatc
      961 ctcagccggg gccgtgatgt gataggcgtc gcccgacaag ccgtatccaa tgacctcggc
     1021 atagatccgc gctccgcgag cacgggcgtg ctccaactcc tcgagcacga caatgccggc
     1081 gccttcgccc atgacgaagc catcgcggtc tttgtcgtaa gggcgagatg cccgctccgg
     1141 ctgatcattg aaattcgtcg acagcgcacg gcaggctgca aacccggcga tggcaagccg
     1201 acaaatcggg ctttcggtgc caccggccac catcacgtcg gcatcaccca acgcgatcag
     1261 tcgcgcggca tcgccgatgg cgtgcgcccc ggtcgagcat gcagtaacga ccgcgtggtt
     1321 gggccctttg agctgatgcc tgatcgatat ctgccctgag gccagattga tgagccggcc
     1381 cggaatgaag aacggactga ccctgcgcgg ccctttttcc tgaagcagaa tagccgtatc
     1441 ggcgatgccc tgaagcccac caataccaga gcctatcaga accccggcgc gttcctgctc
     1501 ctcggtcgtg ctcgccttgt agcccgcgtc ggccaatgcc tgatcggccg ccgccatggc
     1561 atagatgatg aagtcgtcga ccttgcgctg ctccttcggc tccatccagt cgttcggatt
     1621 gaatgtgccg tccgtcccgt cgccacgcgg cactatgcag gcgatctgac atgacagatc
     1681 ggaaacgtcg aacgtctcca cccgacgcgc gccgctttgg ccggccagca gccgagacca
     1741 tgtcgcctcg acgccgcaac ccagcggagt gacgaggccc aaacctgtga tgacaacccg
     1801 tcgcatgcgc aatccttcac cggcggcgtc ccggcaggcc cggttacgcc accgaccatc
     1861 ttgcagagcc tttctgccac caagggagcg gcggcagatt cacgctctca agccgcgttc
     1921 gcgttcttct cgaggaactt gaccgcgtcg ccaacggtca gaatgtgctc ggcggcgtca
     1981 tccgggatct cacagccgaa ctcctcctcg aaagccatga cgagctcgac cgtatcgagg
     2041 ctgtccgcac cgagatcatc gatgaaggag gcgttctccg tcaccttttc cgcttccacg
     2101 ccgaggtgct cgaccacgat ctttttcacg cgctcggcaa cgtcgctcat agcatttccc
     2161 tcgtatcgcc agtcttacct tgagatccct gattgtcgcc ctgatcgctt caaatcggcc
     2221 aaggcggagt ttccgagcgg gctaccagag tcacggtgcg atgaaatcga aatcgcctga
     2281 tctcggcagc tttttcccca gtactcctca cgaaagcagg cgctcctaac acacttcaca
     2341 tcgctttacc agcttcgcga tgtccctgcc gaacaagttc tgggtgacgc tctaagccct
     2401 tgctgtgaaa tggttttacg atcagatcat ggccatgccg ccgttcacgt gtagagtttg
     2461 accggtcata tagcgcgcct cctcgcttgc caagtagagg gctgcggcag cgatgtcgtc
     2521 cggctcgccg aactttgccg caggaatgtg ctgggctatg gcctcgacct gcttttcagt
     2581 cagtacgtcg gtcatagccg tcttaatgaa accgggtgcg atgcaattgg ctgtaatgcc
     2641 gcgggatgca acctcgcgag ccaatgattt ggtcatgccg atcatgccag ccttggctgc
     2701 agcgtaattg ccctggcctg ggttgccgat aatgccagaa atggacgtga tgttgatgat
     2761 ccgcccccac tggcggcgca tcatattccg caaagccgca cggcacagga tgaaggtggc
     2821 cgtgagattg acggcgatca cctcgtccca ctcttcatcc ttcatgcgaa cgaaaaggtt
     2881 gtccttggtc acgccggcgt tgttgaccaa aatatccagt gtgccgagct tggcctcggc
     2941 ttcaccggcg agcttctgga cggcggcgcg atcactcagg tcgcagggga cgatatgaac
     3001 gcgctcgttg agctcattgg ccaaggcctc tagccggtcg attttcgtcc cggagatcgc
     3061 gaccgatgcg ccagcggcat gcagcttccg cgcaattgcg ctgccgatgc ccccgctcgc
     3121 cccagttatg agcgcacccc tgccggtcaa attgaacatt ccgcattacc tcccgagtgc
     3181 cgcgagtgcc tcgtccacgt ccgcaggcga gccgacggaa accgcgttgg cgtccttcaa
     3241 aattcgcttg atgaggcccg tcaatacctt acccgccccg atctcgtaga agtcggtaac
     3301 gccgagctca ctcattttca gcaccgattc gcgccagcgc acggtgcccg taacctgctc
     3361 cacaaggcgc ttcctgatct cttccggatc gctgatcggc tccgcgagga cattggaaac
     3421 gaccggcacc acgggcctac ggatttcaac ttttcccagc tcctcagcca tcacatctgc
     3481 tgccggtttc ataagggagc aatggaacgg agcgctcacc ggcaggggga tggcccgacg
     3541 taccccgtgc ttgcggccga cttcagcgac tcggtcgatg gcggatttat gacccgaaag
     3601 cacgacctgg gccggctcgt tatcgttagc
//
LOCUS       DASUMG010000347         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_768257, whole genome shotgun sequence.
ACCESSION   DASUMG010000347 DASUMG010000000
VERSION     DASUMG010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768257"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..471)
                     /locus_tag="VFX46_05735"
     CDS             complement(127..471)
                     /locus_tag="VFX46_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HEX5600114.1"
                     /translation="MELVISITIGVLTGSGLWLILRPRTFQVIVGLSLLSYAVNLFIF
                     GMGRLRVGKAPVLDSIVPPYLAGYADPLPQALVLTAIVISFAMTALFLVVLLAARGLS
                     GTDHVESVERGP"
     gene            complement(471..>2316)
                     /gene="mbhE"
                     /locus_tag="VFX46_05740"
     CDS             complement(471..>2316)
                     /gene="mbhE"
                     /locus_tag="VFX46_05740"
                     /inference="COORDINATES: protein motif:HMM:NF015970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HEX5600115.1"
                     /translation="RDIRQLGGLYRAMPITATLAMVAAAAMAGVPLLNGFLSKEMFFA
                     ETIETHVVSALDDALPYLATLAGVFSVAYSLRLIHQVFFGPPANNLPRTPHDPVAWML
                     LPVAFLVLMCILVGVAPQAVVGGFLKVAIIPVVGLPTPKYSLTVWHGFTPAFFMSLVA
                     LVGGVSLYIVLHRYLAGDVEGAPILRRLKGRRLFERAVALLSWRGARRLVQALSSNRL
                     QPQIALLITVALLAAVWPLFQNSITLRLPKLSEVDVSFLLVWVVGCLCAIGAALLAKY
                     HRFAAVVLMGGAGLSTSITFAWGAAPDLALTQLLVEIVTTVLLLLGLRWLPKQPKEVD
                     PVSPVVAARRFRDFIIAVVGGLGVGALAYAIMTRPLTFSVSSKFLELAYPEGGGRNVV
                     NVILVDFRAFDTLGEITVLGIVAITVYALLRRFRPAPDSVALPDQKYFQNLFDEERKK
                     ARKDQITSHYMYVPGLILRLLFPIVSMVSVFLFLRGHDLPGGGFVAGLTFAIAFILQY
                     MAGGARWVEARLTVLPVRWIGFGLLAAGGTGIAAALFGRPFLTSAFQYVDLPLLGSIP
                     MASALLFDLGVYALVLGATVLMLIALAHQAIRIPRSTSKAEQVTEGAA"
BASE COUNT          549 a          710 c          735 g          322 t
ORIGIN      
        1 gcgccaatgt cgcgagcacg ttgatgcccg cgacgacttc gcggcgccgc ccctcgaaca
       61 tcagcatcgc cccgccagca aggagcggga tgacaatcgg cgcaatgacc aggtgatcgg
      121 cccaggtcat ggccctcgct ccacactctc gacgtggtca gttccggaca gccctcgggc
      181 tgcgagcaga acgacgagaa acagcgcagt catcgcgaag ctaatgacga tcgccgtgag
      241 gacgagcgcc tgtggcagcg gatctgcata gcctgcgagg tagggaggga cgatcgaatc
      301 gaggaccgga gccttgccga cccgcagtcg ccccatgccg aaaatgaaaa ggttgaccgc
      361 gtacgacagg agcgacagcc cgacgatcac ctggaacgtg cgcgggcgca gaatgagcca
      421 cagccctgat ccggtaagga cgccgatggt tatggagatg acaagttcca tcaggctgct
      481 ccttcagtga cttgctccgc cttgctcgta gagcgcggaa tgcggatcgc ctggtgagca
      541 agggcgatca acatgaggac ggtggcacca aggacgaggg cgtagacacc gagatcgaag
      601 agaagagcag acgccatcgg aatggagcca agaagaggca gatcaacgta ttggaaagcc
      661 gaagtgagga acgggcgccc aaacaatgcc gcggcaatcc ctgtgcctcc ggccgcaagc
      721 agcccaaagc cgatccagcg caccggcagc acggtgagac gcgcctccac ccaacgggcg
      781 ccgccggcca tgtattgaag aatgaaggcg atggcgaacg tcaacccggc gacgaaaccg
      841 ccacccggca gatcgtgacc acgcaggaaa aggaacaccg agaccatgct gacgattggg
      901 aagagtagcc gcaaaatgag gccagggacg tacatgtagt gagaagtgat ctggtccttg
      961 cgggccttct tccgctcttc gtcgaacaag ttctggaagt acttctggtc gggtaaggcc
     1021 acgctatccg gcgcaggacg gaaacggcgc agaagggcgt agaccgtaat ggctacgatc
     1081 ccgagcactg tgatctcccc cagcgtgtcg aaagcacgga aatcgaccag aatgacgttg
     1141 acgacgttgc gtccgcctcc ttccgggtac gccagctcga ggaacttgga ggagacgctg
     1201 aaggtcagag gccgcgtcat gatcgcgtag gccaaggccc ctacgccaag gccaccgacc
     1261 accgcgatga tgaagtcgcg gaaccgtcgc gcagcgacga ccggactgac cgggtccacc
     1321 tccttcggct gcttaggcag ccagcgcagg ccgagcagca gcagaactgt ggtgacgatc
     1381 tcgacaagga gctgcgtgag cgcgaggtcc ggcgcggccc cccaagcgaa cgtgatgctg
     1441 gtcgagaggc cagcccctcc catcagaacc accgcggcga accgatgata cttcgccagg
     1501 agggcagcac caatcgcgca gagacatccc actacccaaa cgagcaggaa gctcacgtcg
     1561 acctcgctca gcttgggaag ccggagggtg atcgagtttt ggaacagcgg ccagacggcg
     1621 gccagcaaag cgacggtaat cagcagcgcg atctgcggct ggagccggtt cgagctcagg
     1681 gcctgcacga ggcgtctcgc gccgcgccac gagagcagcg caactgcgcg ctcgaagagt
     1741 cggcgcccct tcagccgacg cagtatcggc gccccttcga cgtctcctgc gaggtatctg
     1801 tgcaggacga tatagagcga aaccccgccg accaaagcaa ccaggctcat gaagaacgcc
     1861 ggcgtgaacc cgtgccatac agtcagacta tattttggag ttggcaggcc aacgacgggg
     1921 atgatggcga cctttagaaa cccgccgacg accgcctgtg gcgccacgcc cacgagaatg
     1981 cacatcagca caagaaaggc gaccggcagc agcatccacg caaccgggtc gtgcggcgta
     2041 cgcggcagat tgttggcagg gggaccgaag aacacctggt ggatcagacg aagcgaataa
     2101 gccacgctga acacgccggc gagcgtcgcg agatagggca gcgcgtcgtc caatgccgaa
     2161 acgacatgcg tttcgatcgt ctcggcaaag aacatctcct ttgatagaaa tccattcagc
     2221 agcggaacac ccgccatcgc cgcggctgcg accatggcca gtgtggccgt gatcggcatg
     2281 gcacgataga gccctcccag ctggcgtatg tcgcgg
//
LOCUS       DASUMG010000348         3887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_527914, whole genome shotgun sequence.
ACCESSION   DASUMG010000348 DASUMG010000000
VERSION     DASUMG010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527914"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFX46_05745"
     CDS             <1..261
                     /locus_tag="VFX46_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5600116.1"
                     /translation="AKDGQLHPMQEAFREHHALQCGYCTPGMIMAAIDIVNRKGTQLD
                     ERTIREELEGNLCRCTGYHNIVKAIAAGAAAMAAGQYRQAAE"
     gene            284..2650
                     /locus_tag="VFX46_05750"
     CDS             284..2650
                     /locus_tag="VFX46_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5600117.1"
                     /translation="MTSTGIGVPVRRREDFRFITGSGQYVDDISRPGQAYAYFVRSPH
                     AHAIIKNIDLSAALKAPGVLAIYTGKDLAADKIGGLICGWMIHSKDGTPMRVGPHPAL
                     ATEKVRYVGDHVAVVIAETLNQARDAAELVAVDYEVLPAVVDLATAQDPGQPQIHAEA
                     PNNTVFQWHLGDKDAVDAAFAKAAHVTKLEIVNNRLIPNAMEPRAAIGEYDRGQDSYT
                     LYTTSQNPHVARLVLSAFVGIAPEHKLRVIAPDVGGGFGSKIFIYAEETVCLWAARRV
                     GRPVKWTADRTEAFLADAHGRDHLTTAEMAVDGSGKILALRVKTKANVGAYLSTFASS
                     IPTYLYAPLLSGQYDIPAIYCEVDAVYTNTTPVDAYRGAGRPEATFVVERLVEVTARQ
                     LGRDPADFRMQNFIKSFPHQTPVILTYDAGDYAACMKKALELADYANFPQRKAESERK
                     GLLRGIGFSAYIEACGLAPSRAAGSLGAGVGLWESAEVRVNPIGTVEVFTGSHSHGQG
                     HETTFAQLVSHRLGIPMDQISIIHGDTEKVQMGMGTYGSRSGAVGMSAISRALDKVVA
                     KATKIAARCMEVPEDTVDFNDGVFSARGTNKSMTFGEVALQAYTAHKFDSSEIEPGLK
                     ESSFYDPTNFTFPSGVHIAEVEIDPETGVTRIDRWTAVDDFGVIINPMIVEGQVHGGV
                     AQGIGQAMLERAVYDSSGQLLTASFMDYCMPRADDLPSFTVAMTNTPCPSNPLGVKGC
                     GEAGAIAAPAALINAITDALGHENISMPATPQAVWRAAQSSVRKMAAE"
     gene            2663..3463
                     /locus_tag="VFX46_05755"
     CDS             2663..3463
                     /locus_tag="VFX46_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5600118.1"
                     /translation="MYAFDYQRPSTVSEAATLLADGSGAKLLAGGQTLIPTMKQRLAS
                     PPLVVDLRDVAELKGIERTEDRLVIGAMTTHAQVAESSVVAVAISGLCKVAESIGDPH
                     VRNRGTLGGSLANNDPAADYPAAVLALDATVHTNKREIAADDFFQGLFTTALEEDEII
                     TRVSFPIPERFAYAKFPNPASRYAMVGVAVAKGPQGVRVAVTGAGQNGVFRATDMEAA
                     LANSFTPDALKGITVDPSLMNSDIHADAAYRAHLVTVMARRAVEAAAA"
     gene            3503..>3887
                     /locus_tag="VFX46_05760"
     CDS             3503..>3887
                     /locus_tag="VFX46_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEX5600119.1"
                     /translation="MAVNMSGEVILAADRERVWAMLNDPEVLKICIPGCETFEPDGEN
                     TFRAVARVKIGPVNARFKGKVSLSDIDAPNSYKISGEGEGGVAGFAKGGAAVRLEPTE
                     DGGTRLIYDVEANVGGKIAQLGSRLI"
BASE COUNT          743 a         1251 c         1240 g          653 t
ORIGIN      
        1 gcgaaggacg ggcagctcca tccgatgcag gaggcattcc gggagcatca cgccttgcag
       61 tgcggttact gcacgcctgg catgatcatg gccgccatcg acatcgtgaa ccggaagggg
      121 actcaactcg acgaacgcac cattcgcgag gagctcgaag gtaatctctg ccgctgcacc
      181 ggctatcaca acatcgtcaa agccatcgcg gcaggcgctg cagccatggc ggcgggccag
      241 taccggcagg ccgcagagta ggcgcacggg aggagacgac accatgacca gcacgggcat
      301 tggcgttcca gtacggcgcc gtgaagattt ccgcttcatc acgggcagcg gtcagtatgt
      361 cgatgacatc agccggccgg gtcaggccta cgcgtatttc gttcgctcgc cccacgcgca
      421 cgcgatcatc aagaacatcg atctgagcgc cgctttgaag gcaccggggg tgctcgccat
      481 ctacaccggc aaggacctcg cggcggacaa gatcggcgga ctgatctgcg ggtggatgat
      541 ccactcgaag gacggcacac cgatgagggt ggggccgcat ccggcgctcg ctaccgaaaa
      601 ggtgcgttat gtcggcgacc atgtggccgt ggtcatcgcc gagacgctca accaggcgcg
      661 ggacgcggcg gaactcgttg cggtcgacta tgaggtgctc cccgcagtcg tcgatctcgc
      721 cacggcacaa gaccccggcc agccgcaaat ccatgccgag gcgcccaaca acacggtgtt
      781 tcaatggcac cttggcgaca aggacgctgt cgatgcggcc tttgccaaag ccgcgcacgt
      841 cacgaagctc gagatcgtca acaaccgcct gattccgaac gccatggagc cgcgggcggc
      901 cattggcgag tacgatcgcg ggcaggattc ctacacgctc tacacgacca gccagaaccc
      961 gcacgttgcg cgtctcgtgc tctccgcgtt cgtcggcatc gcacccgagc acaagctacg
     1021 ggtcattgcg ccggacgtcg gcggtggttt cggctccaag atcttcatct acgccgagga
     1081 gacggtttgc ctctgggctg ctcggagagt cgggcgcccc gtgaagtgga cggcagaccg
     1141 cacggaagct ttcctcgccg atgcccacgg ccgcgatcat ctcacgacag cggagatggc
     1201 cgttgacggt tcgggcaaga tcctggcgct gcgcgtgaag acgaaggcaa acgtgggtgc
     1261 ctatctctcc acctttgctt cgtcgatccc gacttatctc tacgcgcccc tgctctccgg
     1321 ccagtacgac attcccgcga tctactgcga ggtggacgcg gtctacacca atacgacgcc
     1381 ggtggacgcc tatcgcggtg ccgggcggcc cgaggcgacg ttcgtcgtcg agcggctggt
     1441 ggaggttacg gcgcggcaac tcggccgcga tccggcagac ttccgtatgc agaacttcat
     1501 caagagcttc ccgcatcaga ccccggtcat cttgacttat gacgcaggcg actacgcggc
     1561 ctgcatgaag aaagcgctcg agctcgccga ttacgccaac ttcccgcagc gtaaggccga
     1621 gagcgagcgg aaaggcctgc tccgcggcat cggcttctcg gcctacatcg aggcgtgtgg
     1681 ccttgccccc agtcgagcgg ccgggtcgct cggcgctggc gtgggcctgt gggagtcggc
     1741 cgaggtccgt gtcaatccga tcggcacagt cgaggtgttc accggctcgc acagccatgg
     1801 ccagggccac gagacgacgt tcgcgcagct cgtctcgcat cgcctcggca tcccgatgga
     1861 tcaaatctcg atcatccacg gcgacaccga aaaggtgcag atgggcatgg gcacctacgg
     1921 ctcgcggtca ggcgcggtcg gcatgtcggc catttccagg gcactcgaca aggtggtcgc
     1981 caaggcaacc aagatcgctg cgcgttgcat ggaggtgccg gaggatacgg tcgatttcaa
     2041 cgacggcgtc ttctcggccc gcggcaccaa caagtcgatg acgttcggcg aggtcgccct
     2101 tcaggcctac accgcacaca agttcgacag ctcggagatc gaacctggcc tgaaggaaag
     2161 ctcgttctac gatccgacga acttcacctt cccgtcgggc gtgcacatcg ccgaggtgga
     2221 gatcgatccc gagacgggcg tgacgcgcat cgaccgttgg acagcggtcg acgatttcgg
     2281 cgtcatcatc aacccgatga tcgtcgaggg gcaggtccac ggtggcgtcg ctcaaggaat
     2341 cggacaggcg atgctcgagc gcgcagtcta cgactcgtcc ggccagctcc tgacagcgag
     2401 cttcatggac tactgcatgc cgcgggctga cgacctgccc tcgttcaccg tcgccatgac
     2461 caatacgccg tgcccgtcca atccgcttgg cgtcaaaggt tgtggtgagg ccggcgcaat
     2521 tgcggcgcct gctgcactca tcaacgcgat caccgatgcg ctggggcacg aaaacatctc
     2581 catgcccgcg acgccgcaag ctgtctggcg cgcggcccaa tcctcggtgc ggaagatggc
     2641 ggcggaataa gggaggcgcg agatgtacgc ttttgactat cagcgaccca gcaccgtaag
     2701 cgaagcggct acccttctgg ccgacggctc tggcgccaag ctgctcgcgg gcggccagac
     2761 gctgatcccc accatgaagc agcgcctcgc gagccctcct ctcgttgtcg acttgcgcga
     2821 cgtcgcggag ctcaaaggca tcgagcgcac cgaggaccgg ctcgtcatcg gagcgatgac
     2881 cacgcatgcg caggtcgccg aaagctccgt ggtcgccgtc gcgatctctg gtctctgcaa
     2941 ggtggcggaa agcatcggcg atccccacgt tcggaaccgc ggcaccctcg gcggctcgct
     3001 ggcgaacaac gatccagctg ccgactatcc agcggcggtg ctggcgctcg atgcgacggt
     3061 tcacacgaac aagcgggaaa tcgcagccga tgatttcttc cagggcctgt ttacgacagc
     3121 gctcgaggag gatgagatca tcactcgcgt gtcgttcccg atcccggagc ggttcgctta
     3181 cgccaagttc ccgaacccgg cctcgcgcta tgcgatggtg ggcgtcgcgg tcgcgaaggg
     3241 accacaaggc gtgcgcgtcg ccgtgacggg cgcaggccag aacggcgtct tccgcgcgac
     3301 ggacatggaa gcggcgctgg cgaactcgtt cacgccggat gctctcaaag gcatcacggt
     3361 cgatccgtcg ctcatgaact ccgatatcca tgctgatgcc gcataccgcg ctcacctcgt
     3421 gacggtgatg gcacggcgtg cggtggaagc cgctgcggcc tgaacttaca gccagggaat
     3481 tgcagatcgg atcgcttaag atatggccgt caacatgagt ggcgaggtga tcctcgcagc
     3541 cgaccgggaa cgcgtttggg caatgctcaa cgatcccgag gtgctcaaga tctgcattcc
     3601 cggctgcgaa acgttcgagc cggatggcga gaacaccttc cgggccgttg cgcgggtcaa
     3661 gatcgggcct gtcaatgcgc gcttcaaagg caaggtcagc ctgagcgaca tcgacgctcc
     3721 gaacagctac aagatcagcg gcgaaggcga aggcggtgtt gccggcttcg ccaaaggcgg
     3781 cgctgcggtg cgcctcgaac cgacggagga tggcggcacg cgcctcatct atgacgtaga
     3841 ggccaacgtc ggcggcaaga tcgcccagct tggctcgcgc ctcatca
//
LOCUS       DASUMG010000349         2595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_848744, whole genome shotgun sequence.
ACCESSION   DASUMG010000349 DASUMG010000000
VERSION     DASUMG010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848744"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFX46_05765"
     CDS             complement(<1..304)
                     /locus_tag="VFX46_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5600120.1"
                     /translation="MDMQIEGLRVLVTAGAAGIGLEIARAFIREGAKVHVCDIDRAAL
                     EALSKTDPQITWSVCDVADRAQVAVMFDEALKALGGLDCLVNNAGIAGPTGCVEEIN"
     gene            419..1357
                     /locus_tag="VFX46_05770"
     CDS             419..1357
                     /locus_tag="VFX46_05770"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEX5600121.1"
                     /translation="MPKVAIIGAGLIGRAWAMVFARAGWDVAVWDGVEGVAAKALELI
                     SQGLADLKRHELIDDPEAAARRVRAAQSFEDALSGADLVQENTPETLEMKREIFARLD
                     AVAESKTILASSTSSLVTSLYTENLKGRSRCLVAHPVNPPHLVPTVELCGAPWTSPEV
                     MQRAREIYRSVGQVPIHVKREVDGFVLNRLQAALLSEAFRLVEEDVVTPQDLDETIRN
                     GLGLRWAFMGPFETIELNAPGGIPDYCRRYSPFFRRLINDQPKASVWDEDKTNAVAAA
                     WGPTPDPQKHAAKTRWRDEQLAALAAHKRKMKPFSE"
     gene            complement(1498..>2595)
                     /locus_tag="VFX46_05775"
     CDS             complement(1498..>2595)
                     /locus_tag="VFX46_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HEX5600122.1"
                     /translation="VEDVLACAGDLRSITKEFYLSTVEVNSCKCSNVHEIGRDLLEVL
                     DRLDDIGDKLGLSFAATGCHPFARYSDCIITPTPRYNELIDRNQWLTRRMTVYGMHVH
                     LGMSSGDECIRFNNFFLHFLPHLLALSASSPFWQGEDTGLASCRPTTYEALPTAGQPY
                     VVHSWQEFGQLCSTLKACNAIQSLKDLWWDLRPSPALGTLEIRICDGLASLSETLAVV
                     AFVHALAHWFRDHASWLERVPPPPRWLARENKWRAMRYGLDAKLVTNLDGSVKPLRDD
                     ILEWCERISGKVAALGYEPYIATLRRILTEGNSSTRQRAVYARTGSLHQVVQHNIAEF
                     CARAPRWATSEATPPPRSQLELSLDQVDDGA"
BASE COUNT          514 a          803 c          786 g          492 t
ORIGIN      
        1 gattgatctc ttcgacgcag cccgtagggc cagcgatgcc agcgttgttg acgaggcagt
       61 cgagaccgcc gagcgccttc aatgcctcat cgaacatgac ggcgacctga gcccggtcgg
      121 ccacatcgca aacgctccag gtaatctgcg gatcggtttt cgagagggcc tcaagtgcag
      181 cccggtcgat gtcgcaaacg tggaccttgg ccccctctcg gatgaacgcg cgcgcaatct
      241 ccaatccgat tcccgcggcc cctgccgtca ccagcacccg caagccctcg atctgcatat
      301 ccatgatacg agcctttcct ccgcctatga tcttgccccg ttttcattat tgcatggggt
      361 ggttagcttg agccaccgtg gactcaagca gcgcacggaa cgacaggaga ggaaacgcat
      421 gccgaaggtg gcgataatcg gggccggctt gatcggtcga gcctgggcga tggtgttcgc
      481 ccgagccggc tgggatgtgg ccgtgtggga tggcgttgag ggcgtagcag cgaaagccct
      541 ggaactcatc tcgcaaggtc tcgcggacct caaacgccac gagctgatcg atgaccctga
      601 agctgccgcg cgccgcgttc gtgctgctca gagcttcgag gatgctctat ccggagccga
      661 tctcgtccag gagaacacgc ccgaaacgct cgaaatgaag cgcgagatat tcgctcgtct
      721 cgacgccgtg gccgagtcca agactattct cgcctcgtcg acctcctcgt tggtcacttc
      781 gctttatacg gaaaatttga aaggccgcag ccgctgcctt gtcgcccacc cggtcaaccc
      841 tccgcatctc gtgccgaccg tcgagctgtg cggcgctcca tggacgtcgc ccgaggtcat
      901 gcagcgtgcg cgggagatct atcgttctgt cgggcaggtg ccgatccacg taaagcggga
      961 agtcgacggc ttcgtgctca atcgtctgca agcagcattg ctctctgagg ccttccggct
     1021 cgtggaagag gacgtcgtca caccgcagga tctcgacgag acgatccgca acggcctcgg
     1081 cctgcggtgg gccttcatgg ggccgttcga gactattgag ctcaatgcac ctggtggcat
     1141 tccggattac tgccggcgtt acagcccgtt cttccgccgc ctcataaacg atcagccgaa
     1201 agcttccgtt tgggacgagg acaagaccaa cgcggtcgcc gctgcttggg gcccgacgcc
     1261 ggacccgcag aagcatgccg ccaagacgcg ctggcgcgat gagcagctcg ccgcgctcgc
     1321 agcccacaag cggaagatga agcccttttc tgagtgagag gctttgaaac tagaaagccg
     1381 gttcttccaa cctaaccctg tagggcaagg catcgatcca cgtgaaacgt tcggtccgag
     1441 ccatttccgg ccaatggcgg ctacctggga tcgtgaccgc ccaagcagac aggagcacta
     1501 cgcaccatca tcaacttgat cgaggctgag ctccaactga gatcgcggcg ggggcgtcgc
     1561 ctcgcttgtc gcccagcgcg gagcgcgggc gcagaattcg gcaatgttgt gctgcacgac
     1621 ctgatgcagc gagcccgtac gggcgtatac ggcgcgttgg cgcgtggaac tgttgccttc
     1681 ggtcaaaatc cgccgaagag tggcgatgta gggctcgtat ccgagagccg ccaccttgcc
     1741 ggaaatccgc tcacaccact cgaggatatc gtcgcgcagc ggttttacgc ttccatcaag
     1801 gttggtaacg agcttggcat cgagcccgta gcgcatggct cgccacttgt tctcgcgtgc
     1861 cagccatcgg ggtggcggcg gaactctttc cagccaactc gcgtgatcgc ggaaccaatg
     1921 cgccaatgca tggacgaagg ccaccacagc cagggtttcc gacaggctcg ccagcccatc
     1981 gcagatccgt atctccagcg tacccagcgc tgggcttggc cgaaggtccc accaaagatc
     2041 cttcaaagat tggatggcgt tgcaggcctt gagcgtgctg cacagctggc cgaattcctg
     2101 ccaggaatgc acgacgtagg gctgccccgc cgtgggtagc gcctcatagg tggtgggccg
     2161 gcacgaggcg agccctgtgt cctcaccttg ccaaaacggc gagctggctg acagcgccag
     2221 caagtgaggc aggaagtgca agaagaagtt gttgaaccgg atgcactcgt ccccgctgct
     2281 catcccaaga tgcacgtgca tgccgtaaac ggtcatgcgg cgcgtcagcc actggttccg
     2341 gtcgatgagc tcgttgtacc gaggtgtggg ggtgatgata cagtcactgt agcgtgcgaa
     2401 gggatggcag cctgtcgctg cgaacgagag gccaagctta tcgccgatat cgtcaagccg
     2461 atcgaggacc tccaatagat cacgcccgat ctcatgaaca ttggagcatt tgcaagaatt
     2521 cacctcgacg gtgctgaggt agaactcctt cgtgatgctt ctgagatcac cagcgcaggc
     2581 cagcacgtcc tcgac
//
LOCUS       DASUMG010000350         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1010213, whole genome shotgun sequence.
ACCESSION   DASUMG010000350 DASUMG010000000
VERSION     DASUMG010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010213"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1779
                     /gene="araD"
                     /locus_tag="VFX46_05780"
     CDS             52..1779
                     /gene="araD"
                     /locus_tag="VFX46_05780"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HEX5600123.1"
                     /translation="MTSRKRPEELRSHRWLGVNDLRAFGHRSRLRQGGFDAADFRGKP
                     VIGIINTWSDINFCHTHLRDRAEDVKRGVLQAGGFPIELPAMSLSEPFVKPSTMLYRN
                     LLAMETEELLRSHPIDGVVLMGGCDKTTPALIMGAVSMGLPAIYVPAGPQLRGNWRGQ
                     ELGSGSDAWKYWDEKRAGRISEADWAELEGGIARSFGTCMVMGTAATMMAITEALGLA
                     LPGASSIPAADAAHPRMCAATGRRVVEMVWEDLTPGKILTSAAFDNAIRVHMAMGGST
                     NAIIHVVAMARRAALKLDMNRFDEISREIPVLANVRPSGKFLMEDFFYAGGLRALMAE
                     LRSALDLSCLTVTGKSIGDNVAGAEVYKRDVIHPFNDPVSREGATAVLKGNLAPRGCV
                     MKPSAAEKRLLKHRGKVIAFEDYNHMAREVERDDLDVTPDHILVLKNGGPKGGPGMPE
                     WGMLPIPKKLVKQGVRDMLRISDARMSGTSYGACILHVAPESFVGGPLAFVKTGDEIE
                     VDVPARRIHLHVSDEEIARRRASWTQPAPRYPRGYGALYSEHIGQADEGCDFDFLAPL
                     GAVPEPEIH"
     gene            complement(1789..>2185)
                     /locus_tag="VFX46_05785"
     CDS             complement(1789..>2185)
                     /locus_tag="VFX46_05785"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5600124.1"
                     /translation="GPALEQPFHALTTAWLLAAAVLVALGLGFSALARFSLGRNWSAQ
                     VTVKHDHELIRSGPYALVRHPIYTGALLALLGTAISVDKWRALVGLALMVVAFLRKLT
                     IEERFMAEQFGKAYARYKAEVPALIPFVV"
BASE COUNT          424 a          655 c          705 g          401 t
ORIGIN      
        1 cgtgcacgcg gccaacctgt cgataaacta gcggactgcg catcggaaag tatgaccagc
       61 cgtaaacgtc ccgaagagct gcgcagccat cgctggcttg gcgttaacga cctgcgcgcc
      121 ttcgggcatc gctcgcgcct gcgtcagggt gggttcgatg ccgccgattt ccgcggcaaa
      181 ccggtaatcg gcattatcaa tacctggagc gatatcaact tctgccacac gcacctgcgg
      241 gaccgcgccg aggacgtgaa gcgtggtgtg ctgcaagcgg gcggctttcc gatcgagctc
      301 ccagcgatgt cgctgtcgga gcccttcgta aagccctcga cgatgctcta ccgcaatctc
      361 ctggcgatgg agacggaaga gctgctgcgc agccatccga tcgacggggt cgtactgatg
      421 ggcggctgcg acaagaccac cccggcgctg attatgggcg cggtgagcat ggggctgccc
      481 gcgatttacg tgcctgccgg tccgcagttg cgcggcaact ggcgcggcca ggaactcggc
      541 tccgggtccg atgcctggaa atattgggat gagaagcgcg cgggccgcat cagtgaggcc
      601 gactgggccg agcttgaggg cggcatcgcg cgttccttcg gaacctgcat ggtgatgggc
      661 actgcggcaa caatgatggc gatcaccgag gctttagggc ttgccttgcc gggtgcctcg
      721 tcgatacccg cagcggacgc agcgcatccg cgcatgtgtg cggcgaccgg tcggcgcgtg
      781 gtcgagatgg tttgggagga cctgacgccg ggaaaaatcc tcacgtcggc ggctttcgac
      841 aatgccatcc gtgttcacat ggcaatgggg ggctcgacca atgcgatcat tcacgtcgtt
      901 gcaatggcgc gtcgtgcggc cctcaagctc gacatgaatc gcttcgatga gatctcgcgt
      961 gaaattcccg tgctcgccaa tgtacggcca tccggaaaat tcctgatgga ggatttcttc
     1021 tatgccggtg gattgcgcgc gctgatggct gaactgagga gcgcgctcga tttgtcgtgc
     1081 ctgaccgtta ccggaaaaag catcggcgac aacgtcgccg gagccgaggt ctacaagcgc
     1141 gacgtgatcc acccgtttaa cgatccggtg tcgcgtgaag gtgcaaccgc cgtgctcaag
     1201 ggaaatctgg cgccgcgcgg ttgcgtaatg aagccatcgg cggccgaaaa acggctgctc
     1261 aagcaccgtg gcaaggtgat cgcctttgag gattacaacc acatggcacg tgaggtcgag
     1321 cgcgatgacc tagacgtgac acccgaccac attctcgtgc tcaagaatgg cggtccaaag
     1381 ggcggtccgg gcatgccgga gtggggcatg ctgccgattc cgaaaaagct ggtgaagcaa
     1441 ggcgttcgcg acatgctgcg catttccgat gcgcgcatga gcggcaccag ctacggcgcc
     1501 tgtattcttc atgtggcgcc cgagagtttc gtcggtggcc cgcttgcttt cgtgaaaaca
     1561 ggcgacgaga tcgaggtcga tgtgcctgcg cgccgtatcc atctccacgt ttcggacgaa
     1621 gaaatcgcgc gacgccgcgc cagttggaca caacccgcgc cccgttatcc gcgcggttat
     1681 ggtgcgcttt actcggaaca catcgggcag gccgacgagg gctgcgattt cgattttctc
     1741 gctccgctcg gcgcggtgcc cgagcccgag atccattgag ccgtcggctt acaccacgaa
     1801 cggaatgaga gccggcactt cggctttata acgcgcataa gccttgccga actgttcggc
     1861 catgaaacgc tcctcgatcg tgagcttgcg caggaacgca acgaccatca gcgcaagtcc
     1921 gaccaaagca cgccatttgt cgaccgaaat ggcggtgccc agcagagcca gaagcgcgcc
     1981 tgtgtagatc gggtggcgca caagtgcgta gggcccgctg cggatcaact cgtgatcgtg
     2041 cttgacggtt acctgcgcac tccagttgcg gccgagcgag aagcgcgcca gggcagaaaa
     2101 tcccagcccc agcgcgacaa gcaccgcggc ggccagaagc catgctgttg tgagggcgtg
     2161 gaaaggctgc tccagcgccg gtccc
//
LOCUS       DASUMG010000351         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1010312, whole genome shotgun sequence.
ACCESSION   DASUMG010000351 DASUMG010000000
VERSION     DASUMG010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010312"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..561
                     /locus_tag="VFX46_05790"
     CDS             160..561
                     /locus_tag="VFX46_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEX5600125.1"
                     /translation="MARPPIETRVALAPRLLAALMILFLTMGMARAQDSKPYDDKLMR
                     LSEILGAVHYLRELCGADEGQLWRDRMRELIEAEGTTALRRAQLTRSFNKGYMGYSRT
                     YRACTPSAETAVKRFLSEATDLATELVRNVP"
     gene            683..928
                     /locus_tag="VFX46_05795"
     CDS             683..928
                     /locus_tag="VFX46_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600126.1"
                     /translation="MQTVSEAEDQSDSFKALSLILEAWDEGAEQGIPSELMAYAALFT
                     ALTDLVSAFGEEAVANMTRSLDKRVRQGEFTIYSTTQ"
     gene            complement(954..1619)
                     /locus_tag="VFX46_05800"
     CDS             complement(954..1619)
                     /locus_tag="VFX46_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5600127.1"
                     /translation="MMHLIPNPLIIPIGLIVGVLVSAPVGPVNVLCIQRAIERGFWGG
                     VAAGAGAVLGDGLIALCAALGVGAISGAIEDYRNAIQFIGGLALIGFGIKLYLTMPRI
                     ITGSEADKANSLIEFLWDTPKTFFLTITNPGAVLGLFAIFGGVSSFVEVQSTVDAITM
                     VAAIIAGSFAWWVLLSSLIGRIRHRFAFGRINQINRIAGVLLFGFGAILIGELVLQFA
                     GLL"
     gene            1894..2382
                     /locus_tag="VFX46_05805"
     CDS             1894..2382
                     /locus_tag="VFX46_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600128.1"
                     /translation="MHTTGCTVDGNAPPPLPAPVREMRDAILAAVQSGDIEDLRVAVE
                     WNELRPHVADEAVDDPIAYWKRISVDGEGREILAVLGEILECGYAALPIGNDVENNLV
                     YVWPALAEADLTKLTPQQEVALYRLVSPDQVKTMREAKRWQWWRLAIGADGTWHAFHK
                     AE"
BASE COUNT          525 a          731 c          768 g          479 t
ORIGIN      
        1 aagccggaat atgcctttgt tcgattacga gctgcaggag ttcgagatca cgcgtttacg
       61 ccagctctgg ctgcggttga agcggataat agcgcgctta actactcatc gctcaacggc
      121 agtgctatga ccaccctgcc ccatcaagga agacactaaa tggcaaggcc accgatcgaa
      181 actcgcgtcg cgttggcacc ccgcctcctg gctgcgctga tgatcttgtt tttgactatg
      241 ggaatggcac gcgcccagga cagcaagccc tatgacgaca agctgatgcg tctgtccgag
      301 attcttggcg cggtgcacta cctgcgcgag ctttgcggcg cagatgaggg ccagctctgg
      361 cgcgaccgca tgcgggagct cattgaagca gaaggcacga cggcgctacg ccgggcgcag
      421 ctgacgcgca gcttcaacaa gggatacatg ggttacagcc gcacgtaccg ggcatgcacg
      481 ccttcggccg agacggcagt gaagcgcttc ctttccgagg cgacggatct cgcgaccgag
      541 ctggttcgca atgtgccctg acggcaactc tcctgcttgc gcgagattca ctcttaattg
      601 ttgttgcagc cactcaagtc gagcgttgtg ctagggcttg cgttgtggga atgcacacgc
      661 aagtgcagag gacgatagca ttatgcaaac ggtctccgaa gcagaagacc agagcgacag
      721 cttcaaggcg ctgagcctca ttctggaagc ttgggacgag ggagccgagc agggcattcc
      781 ctcggagttg atggcctacg cagcgctttt cacagccctg accgacctcg tttcagcttt
      841 tggcgaggag gcggttgcca atatgacgcg gagcctcgat aagcgcgtgc ggcaaggcga
      901 gttcacgatc tacagcacga cgcagtagcc gctgctccgg gcagattttg gcgtcacaac
      961 agtccggcga attggagcac cagctcgccg ataagaatgg cgccgaatcc gaaaagcagg
     1021 acgcctgcga tccgattgat ctggttgatt cggccgaaag cgaagcgatg ccggattcgg
     1081 ccgatgagcg aggaaagcag aacccaccag gcaaagctgc cggcgatgat ggcggcgacc
     1141 atcgtgattg cgtccacggt cgactgcacc tcgacgaagc tcgacacgcc accaaaaatg
     1201 gcgaaaagcc cgaggactgc tccggggttg gtgatcgtga ggaagaaggt tttcggtgta
     1261 tcccaaagga actcgatcag ggaattcgcc ttatcggcct cgcttccagt gatgatgcgc
     1321 ggcatcgtca agtacagctt tatgccgaac ccgatgagcg ccaacccgcc gatgaactgg
     1381 atcgcattgc gatagtcctc gatcgcgcca gaaatcgcgc cgacgcccaa ggctgcgcag
     1441 agggcaatca gtccgtcacc cagaacggct ccagcgccgg ccgcgacacc gccccagaac
     1501 ccacgttcga tcgctcgctg gatgcacagc acgttcaccg gccccacggg cgcggaaacc
     1561 agcactccta cgatgagacc tatgggaatg atcagcggat tgggaataag gtgcatcatt
     1621 caaattgagg gggcgcccaa tccgacagtg ctcctcgcga aatcaccaca gctctgatgt
     1681 aacgcctgcc gtacgcggca ttgacggatg gcgtcgtcca gctgagaagc ttgcgcatgc
     1741 acctgcgctg caatggagtc ctcccttatg agttggttaa gcgccccgac cccgcttctt
     1801 cgcggatgcc tattggtaat ttcgctcacc tgtcttgtcc aatggttagc gccgtctagc
     1861 gtcctcttgg cgcaaccgga aatgaatgcg gcggtgcaca cgaccggttg cacggtcgac
     1921 gggaatgcgc cgcctcctct cccggcacct gtccgggaga tgcgggatgc gattctggcc
     1981 gcggttcaga gcggcgatat cgaagacctt agagtggccg tcgagtggaa cgagctcagg
     2041 ccccatgtcg ccgatgaggc ggtcgacgat ccgatcgctt actggaagcg catatcggtc
     2101 gatggcgaag gccgcgagat tctggctgtg ttgggagaga tccttgaatg cggctatgcc
     2161 gccctgccta tcggcaacga cgtcgagaac aatctggtct acgtctggcc ggccctggcc
     2221 gaagcggatt tgacgaagct gaccccgcaa caagaggtgg cgctctacag gctcgtcagc
     2281 ccggaccaag tgaagaccat gcgcgaagcc aagcgctggc agtggtggcg ccttgccatt
     2341 ggtgccgacg ggacgtggca cgcctttcac aaggccgaat aactcaccgc ggcttttgcc
     2401 tggccgcatg tgtgctgatg ggaggcgggt tgcgacgtag ggtcgcttgc ttgctcgtac
     2461 tgaccgagat tttgcgtccc catcaatggg atgtgcctta gtg
//
LOCUS       DASUMG010000352         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_207421, whole genome shotgun sequence.
ACCESSION   DASUMG010000352 DASUMG010000000
VERSION     DASUMG010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207421"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VFX46_05810"
     CDS             complement(<1..535)
                     /locus_tag="VFX46_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5600129.1"
                     /translation="MHPAPEYLPRFKGSGRLADKVVLITGGDSGIGRAVAVAMAREGA
                     DVAFVYLEETKDADDTVQLIEAENRRAVSIRGDIGEEKFCQDAVRQTLEAFGKLDILV
                     NNAAEQHLEDRLEDISAEQLERTFRSNVFGYFYMTKAALPHMCEGSSIINTTSVTAYK
                     GSAHLMDYSATRGAIVSF"
     gene            complement(806..>2664)
                     /locus_tag="VFX46_05815"
     CDS             complement(806..>2664)
                     /locus_tag="VFX46_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5600130.1"
                     /translation="ETARLPEGWSGGIADSSGRVIVQSRDFQFDAASLAALRSREAEG
                     RVEIISQPVPDRLQAITRSGLANWFVTASAAPSLALPQALSWKLFAAAGATLLALSLL
                     LAFYFGRRLAAPIRMTALAATALGKGEAMPSLALHLREVDEVLSALKAASAQRRLAED
                     QLRVAHERMTLALSATEMGMWERDLATNRVTWSEAMYRLFGRTPEEFTGDPDQVLSFV
                     HPEDQPRFRQAYLDAIRGPSDTFEQEFRVVWPSGTVRWLYRRAFVRRGPDGKAASVLG
                     VAIDITERKEAENANAQLAAIVASSSEAILSVSPDGIIATWNAGAEQTFNYSAAEAVG
                     LPLRELFAEEGLADYERIRSAMAEGHTLRLESFCRTKIGQVLEVAIVANPVCCSPKSV
                     SSYSITIRDISARKEHDRHLANVMRELTHRSKNLLAIIQAMARQTALRSDNLADFEAR
                     FSGRLQSLSRSHELLISNDWEGALLSDLVAVQRTAFGPRHSQIRASGPDVFLRPEAIL
                     NIGLALHELASNAERHGALSVSSGRVDLTWSLEHGKGDKPRLMIVWRERGRQPAGALL
                     HRGFGRDVLEHVVPTALGANVVLKAIPDGLLWTLDMPGDHFARGRIPPAPAT"
BASE COUNT          545 a          814 c          776 g          529 t
ORIGIN      
        1 tgaatgaaac gattgctcca cgggtcgcac tgtaatccat caagtgcgca ctgcctttat
       61 aggcagtcac cgaggtcgta ttaataatgc tggagccttc gcacatgtgc ggcaaagctg
      121 ccttggtcat gtagaagtag ccgaacacat tgctgcgaaa agttcgctcc agttgctccg
      181 ccgagatatc ttccaatcga tcctcgaggt gttgctctgc tgcattgttg acgaggatgt
      241 cgagcttccc gaaggcttcg agggtttgcc gtaccgcgtc ttggcaaaac ttttcctcac
      301 caatgtcgcc tctgatggat acggctcgcc tgttctcagc ctcaatgagc tggaccgtgt
      361 cgtcagcgtc tttagtttcc tccaaataga caaaggcgac gtcggctccc tctcgcgcca
      421 tagcgacagc tacggcccga ccgatgccgc tatcaccgcc agtgatcagc actaccttat
      481 ccgctaaacg accactcccc ttgaagcgcg gaagatattc cggcgcggga tgcatcaggt
      541 gctcgcgtcc cggctgatgc tcttgtgtgt gcggcttggg tgttgctggc attggtcttg
      601 cgcccggaaa taactctgac gacttgggcg caacccctgc ggtccgcacc agttcccaac
      661 caaaacttct ggattaattt tcgaaatttc attcgggcca gcaggggaag gagcaacccg
      721 tgattgggcc gaatacgggt tcgagaatac gcggagcgaa aaccagcttc tcgattcttg
      781 atgttaggct tctcgcgacc gttaactaag tcgccggtgc tggcggaatc cggcccctcg
      841 cgaaatgatc gccgggcata tcaagcgtcc aaagcagccc atccggaata gctttgagca
      901 ccacgttggc accgagggca gttgggacga cgtgctcgag gacgtcgcgc ccaaagcctc
      961 gatgcaacag agccccggca ggttggcggc cgcgctcacg ccagacgatc atcagcctgg
     1021 gcttatcgcc tttcccgtgt tccagcgacc aagtgagatc gacgcggccc gaagacacgg
     1081 acagcgctcc gtgccgctct gcattgctgg caagctcgtg cagcgcaagg ccaatgttga
     1141 gaatggcttc cggccgaagg aaaacatccg gaccggaggc gcggatctgt gaatgccgcg
     1201 gcccaaaggc ggtcctttgc actgcgacca aatcgctcaa caaagcgcct tcccaatcat
     1261 tgctgatgag cagctcgtgc gagcgggaaa ggctttgcag ccggcccgaa aaccgcgctt
     1321 cgaaatcagc caagttatcg cttcgcagcg cagtctggcg ggccattgct tggatgatcg
     1381 caagcaagtt tttcgatcgg tgcgtcagct cgcgcatgac attcgcgagg tgccgatcgt
     1441 gctccttgcg ggcggaaatg tcgcgaattg tgatggaata gctcgatacc gacttgggcg
     1501 agcaacacac cggattggcg acgatcgcga cctccaggac ctgtccgatc tttgtccggc
     1561 aaaagctttc cagccggagc gtatgcccct ctgccattgc cgaccggata cgctcatagt
     1621 ccgcgagccc ctcttccgcg aaaagctctc ggaggggtaa tccgacggcc tccgcggcgg
     1681 agtagttgaa ggtctgctcg gcgcctgcgt tccaggtcgc gattattccg tcgggcgata
     1741 cgctcagaat ggcctcgctc gaggaagcta cgatcgccgc aagctgcgcg ttggcgttct
     1801 ctgcttcctt gcgctcggtg atatcgatag caacgcccaa aaccgaggct gcttttccat
     1861 cgggtccgcg ccgcacaaac gcgcgccggt aaagccaccg cactgtgccg gagggccaaa
     1921 cgactctgaa ctcctgctcg aaggtatcgc tggggccgcg gatcgcatcg aggtaagcct
     1981 gccggaaacg cggctggtcc tcaggatgaa caaagctcaa gacttggtct gggtcgccgg
     2041 tgaattcttc gggagtacgg ccgaacagcc ggtacatggc ctcggaccac gttacgcgat
     2101 tggtcgcaag gtccctctcc cacatgccca tttcggtcgc cgagagggcc agcgtcattc
     2161 gctcatgggc aactcgaagc tggtcctcgg ccaatctgcg ctgagcggat gccgctttca
     2221 gggcgctcag aacctcatcc acttcgcgca ggtgcaatgc cagcgacggc atggcttcgc
     2281 ctttgccgag agccgtggca gcaagcgctg tcatacggat cggcgcggca agccttcggc
     2341 cgaaatagaa ggccaggaga agcgaaagcg cgagtagcgt cgcgccggca gcagcaaaaa
     2401 gcttccagga caaagcttgc ggcaacgcca gcgaaggagc ggcgcttgca gtgacaaacc
     2461 agttcgcaag acccgatcgc gtgattgcct ggagcctatc gggtacaggc tgactgataa
     2521 tctctacacg cccctcggcc tccctggacc gcaatgcggc aagagatgca gcatcgaact
     2581 ggaaatcccg cgattgcacg atgacgcggc cgctactgtc cgcgatgcct ccgctccagc
     2641 cctccggcag ccgggctgtc tcga
//
LOCUS       DASUMG010000353         4405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_127551, whole genome shotgun sequence.
ACCESSION   DASUMG010000353 DASUMG010000000
VERSION     DASUMG010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127551"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFX46_05820"
     CDS             complement(<1..561)
                     /locus_tag="VFX46_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEX5600131.1"
                     /translation="MTENRFHPDALSERFEDDEAPTGLFDPAREHDACGVGFIVDLKS
                     RPTHGIIEDALRILENLEHRGAVGADPIAGDGAGILIQIPHAFLAEECAKLGFKLPPP
                     GHYAVGHVFMPRDDRLRRHCETVWNRIVREEGLELIGWRDVPVDNSCLSDSVRATEPV
                     HRQVFIGRPKAIKDDDAFERRLYIVRK"
     gene            complement(1232..1696)
                     /locus_tag="VFX46_05825"
     CDS             complement(1232..1696)
                     /locus_tag="VFX46_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX5600132.1"
                     /translation="MRHLDFAPLYRSTIGFDRLIRLLDEVAGFGDEAASYPPYNIERI
                     GENEYRITMAVAGFGSDEIQVEVKDTALTIRGQKKAEEKKREYLYRGIAQRSFERRFQ
                     LAEYVEVRGASLNDGLLHIDLVRNLPERMRPRVIPIGTAEQAIEADPATTQN"
     gene            1957..2559
                     /locus_tag="VFX46_05830"
     CDS             1957..2559
                     /locus_tag="VFX46_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600133.1"
                     /translation="MSLRMLAAGLVTALPLAMAPATTAVAADLYDPYESKYSGSPYDD
                     PRYADIYGKPKPDHRPYPDEPVEEFYEAPDDVDLEPLYPGPKRYAKPYDEFDDWSDRT
                     RWRKKPDFEPYHPKKAYLPPVDCLSHHELHRELIRDGWSDFRDLKIHGKIAFVNARRP
                     NGTLYRLEVDRCAGEVVSAERVDGHPDSYAWRRREAYPTY"
     gene            2728..3684
                     /locus_tag="VFX46_05835"
     CDS             2728..3684
                     /locus_tag="VFX46_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600134.1"
                     /translation="MQLVSIARNPVPSGAVVGAFEGYDGNPMRFARWEPTRGPTRGTV
                     CVFTGRSEFIEKYFEVVSDLRRRGFAVAIMDWRGQGGSYRPLTNPRKGWVRSFSEYDR
                     DLDCFMEQVVKPNCPTPYYALAHSMGGHILLRIAGRPVSPFERIMVVAPMLRFHDKKV
                     GVSQRVARVYAAIGTACGLGRTYVRGGSDATQDPVVFDGNPLTSDEARFARNRALIEA
                     APHLLLGAPTIGWLNAAYRSMAKLNDPAYAERIAVPLLVAIAGKDVIVDPLATEAFAA
                     RVKLCTTISLPLARHEILQEADEIRGRFWAAFDAYLGVETVS"
     gene            complement(3730..>4405)
                     /gene="hisN"
                     /locus_tag="VFX46_05840"
     CDS             complement(3730..>4405)
                     /gene="hisN"
                     /locus_tag="VFX46_05840"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX5600135.1"
                     /translation="GGFDPVTAADRAAERAISKLLANRFPDHGLEGEEYGHRNPDARF
                     RWIVDPIDGTRAFIIGMPIWGTLIGLLDGETPVIGMMDQPYTGERFWSGQRASYFRGP
                     DGKERRMKTRACASLADAMLSTTDPELFISAKDRRAFRAVRERVRAVRYGGDCYAYCL
                     LAAGLVDIVVETGLKPHDVVALIPIIERAGGQITTWEGEPAVRGGRIVATGDARLHEE
                     ALSLLA"
BASE COUNT          810 a         1391 c         1341 g          863 t
ORIGIN      
        1 ttttcgcaca atgtagagcc gacgctcgaa agcatcgtcg tccttgatcg ccttcggccg
       61 gccgatgaag acctgccggt gcacgggctc cgtcgcccgc acgctgtctg acaagcagga
      121 gttgtcgacg ggcacgtcgc gccagccaat caactcgagg ccttcctcgc gcacgattcg
      181 gttccagacc gtctcgcaat gccggcgcaa ccggtcgtcc cgcggcatga acacgtgtcc
      241 aacggcataa tggccggggg gcggcagctt gaaaccgagc ttcgcacact cttcggcgag
      301 aaatgcgtgc gggatctgga tgagaatgcc ggcaccgtct ccggcgatgg ggtcggcccc
      361 aacggcgccg cgatgctcga ggttctcgag aatacgaagt gcatcctcga taatcccatg
      421 cgtcggcctt gacttgagat cgacgatgaa gccgacaccg caagcatcgt gctcgcgggc
      481 tggatcgaac agacctgtcg gggcttcgtc gtcctcgaac ctttcgctga gcgcgtcggg
      541 gtggaagcgg ttctcggtca tggggtcggc ctctcgtccg gggactcccg gggctcgaac
      601 tcatcacaag ggacacgtcg attgtgcgac gggttgggac agcggtgcac gatcttccta
      661 gagaccggcg atcgctaggc gcgagcgatg gcgctcaggc tcgcgtacgg gggcgttttc
      721 gggtacccgt tgccgtggat atacgttttc ggttcgtaat cgtgcgcacg gtgccggaca
      781 ctgtccgcac catcttccgc acttgttcga accaaactgc ttccacgggc atcctttcgc
      841 gaggtcacgg cctcgccttc aaccataaag gacaggagta ctgacctttc gccggtcaca
      901 taagcagatt tcaggatact gcacaagcga ttccttgcag agcattacga gccttgccgc
      961 tgttccgcgg actccatccc gcctccgcaa cgatcgattg gacgatccgt tcttgcgtca
     1021 aatgcgtcgc gcggatgacg cacgcgctcc gtcacgcgaa gcgcccgtgg ctgcaccgag
     1081 tctcgttgga ggtcgttagc tcaaccgcag caacgagact ggcagcagcc tgctcggaac
     1141 ggtggccgag gcaactgaac caagtgccgg cgacagtaaa agaagcgggc gcttccaaaa
     1201 gaaagcgccc gcctccgttt cacagataac ctcagttttg cgtcgtagcc ggatccgcct
     1261 caatggcctg ctcggcggtg ccaatgggaa tgacgcgggg acgcatacgc tccggcagat
     1321 ttcgtacgag gtcgatgtgt agcaagccgt cattcaggct cgcacctctg acctcgacgt
     1381 attcggcgag ttggaaccgg cgctcaaagc tgcgctgcgc aatgcctcgg taaaggtact
     1441 cccgcttctt ctcttcagcc ttcttctgac ctcggatggt aagggcggta tccttcacct
     1501 cgacctggat ttcatccgag ccaaaaccgg ccacggccat ggtgatccgg tattcgttct
     1561 cgccaatccg ctcgatattg tacggtggat agctggcggc ctcgtcacca aaaccggcaa
     1621 cctcgtccag caatcggatg agccggtcga aaccgatcgt ggaacggtaa agaggtgcga
     1681 agtcgaggtg tctcatcaca agtcctcctg aaagcgactg tggcattgtc tggctctgaa
     1741 ggcccagacg gttgatcggt gcacatcctg aaaaggcctg tgcaccatcg atgaagttgg
     1801 ttcgaggtcg gggactttca aggtccctca ggcgactgcg gatctacgcg gcagcgagcg
     1861 cgttattggt agacaagtcc ttcatctgac gttcatgttc gcgccacgat gctcccggtc
     1921 tttggagtag cacagcgctt cttcggagga ttgatcatgt ccttgcgtat gctggctgcc
     1981 ggtctcgtca ctgctctgcc cttggcaatg gctccggcca ccacggctgt ggctgccgac
     2041 ctgtacgatc cgtacgagtc caagtactca ggctctccct acgacgatcc gcgctacgcc
     2101 gatatctatg ggaagccgaa gcctgaccac cgcccttatc ctgacgagcc ggttgaggaa
     2161 ttctacgagg ctccggacga tgtcgatcta gagccgcttt accccggccc gaagcgttac
     2221 gcgaagccct atgacgaatt cgacgactgg tctgatcgga ctcgctggcg caagaagccg
     2281 gacttcgagc cctatcatcc gaagaaggcc tacctgccgc ccgtggactg tctctcgcat
     2341 catgagctcc accgcgagct tattcgggac ggctggtcgg acttccgcga cctcaagatc
     2401 cacggaaaga tcgcgttcgt aaacgcccgt cggccaaacg ggacgcttta tcggctcgag
     2461 gtcgatcggt gcgcgggcga ggtcgtgagc gccgaacgcg tcgacggcca tccggactct
     2521 tacgcatggc gccggcgcga ggcttatccg acctactgag cgaggagacc ttcggctcac
     2581 acacgcatcg atgttgaaga tgcgtgtgtt ttagtcgacg ggcgcttctg cctgcgctac
     2641 caccttcaca ggatcgtccc cgaagaacga aggccgggag cgggcgtttg tacctgaccg
     2701 aaggcggtcg caagcccggt tcgcatcatg caactcgtca gcatcgcgag aaacccggtg
     2761 cccagcggtg cggtcgtggg cgctttcgag ggctacgacg gcaacccgat gcgcttcgcc
     2821 cgctgggagc cgacgcgcgg ccccacccga ggcaccgtgt gcgtcttcac cggccgtagt
     2881 gagttcatcg agaaatactt cgaggtggtc tccgatctac gccgccgcgg ctttgccgtc
     2941 gctatcatgg actggcgcgg gcagggcggc tcgtaccggc cactgaccaa cccccgcaaa
     3001 ggttgggtaa ggagcttttc ggagtacgac agggacctcg actgcttcat ggagcaagtg
     3061 gtcaagccga actgtccaac gccctactat gcgctcgccc attccatggg cggacacatc
     3121 ttgctccgca ttgccggccg gccggtttcg ccatttgagc gcatcatggt tgtggcgccc
     3181 atgctccgct tccatgacaa gaaagtcggg gtgtcacaac gcgtggctcg cgtgtatgcc
     3241 gcaataggca ctgcgtgcgg gcttgggcgg acttacgtgc gcggtggctc ggacgccacc
     3301 caagatcccg ttgtcttcga cggcaatccg ctgacgtcgg acgaggctcg gtttgcacgc
     3361 aatcgtgcgc tgatcgaggc tgcgccacat ctcctgctgg gggcgccgac catcggctgg
     3421 ctcaacgccg cctataggtc gatggccaag ctgaacgatc ctgcatacgc cgaacggatt
     3481 gccgtgccgc tgctggttgc catcgccggt aaggatgtca tcgtcgaccc gcttgccacg
     3541 gaagccttcg ctgcccgcgt gaagttgtgc acaacgatca gcctgccgct cgcccggcac
     3601 gagatcttgc aagaggccga tgaaatccgg ggccgcttct gggctgcatt cgacgcctac
     3661 ctgggtgtcg agacggtttc ctgatccacc cgagggtcag cttcgaagcc aggtgaggcg
     3721 ctgagccatt tatgccagaa gcgagagagc ttcctcgtgg aggcgcgcat cccccgtcgc
     3781 gacgatgcgg ccgccgcgaa ctgccggttc gccctcccac gtcgtgattt gccctccggc
     3841 gcgttcgatg atggggatga gcgcgacgac gtcatgcggc ttcagaccgg tctcgacgac
     3901 gatgtcgacg agcccagcgg cgagcaggca gtaggcatag caatcgccgc cataccgcac
     3961 agcgcggacc ctctccctca ctgcccgaaa agcgcgccgg tccttcgcgg agatgaacag
     4021 ctcaggatcg gtggtcgaga gcatcgcatc ggccagtgac gcgcaggccc tggtcttcat
     4081 ccgccgctcc ttgccgtcgg gccctcgaaa atagctcgcc ctctgtcccg accagaaacg
     4141 ctcgccggtg tagggctgat ccatcatgcc aatcactggc gtttcgccgt ccaagaggcc
     4201 aatgagcgtg ccccagatcg gcatgccgat gatgaaggcg cgggtgccgt cgatcggatc
     4261 gacgatccag cgaaaccggg catcagggtt gcgatggccg tactcctcgc cttcgagccc
     4321 atggtcgggg aagcgattgg cgagcagttt gcttattgcc cgttccgccg cacggtctgc
     4381 agcggtgacc ggatcgaacc cgccc
//
LOCUS       DASUMG010000354         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_769253, whole genome shotgun sequence.
ACCESSION   DASUMG010000354 DASUMG010000000
VERSION     DASUMG010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769253"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1867
                     /gene="aspS"
                     /locus_tag="VFX46_05845"
     CDS             71..1867
                     /gene="aspS"
                     /locus_tag="VFX46_05845"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX5600136.1"
                     /translation="MHRYRTHTCGALRKGDVGRIARLSGWVHRVRDHGGVLFIDLRDH
                     YGITQIVADPDSKAFKAAETVRAEWVIRVDGLVRERPEGTVNPDLATGEIEVYAHEIE
                     ILSEAKELPVPVFGEPDYPEDLRLTYRFLDLRRETLHNIIMKRLEITRSIRRRMHEAG
                     FNEFPTPILTASSPEGARDFLVPSRLHPGKFYALPQAPQQYKQLLMISGFDRYFQIAP
                     CFRDEDPRADRLPGEFYQLDVEMSFVEQEDVFQTMEPIIRGIFEEFGEGKPVTPVFPR
                     IPYEEAIRKYGTDKPDLRNPIVMQDVTEHFRGSGFKVFANMIEKDPKVRVWAIPAPGG
                     GSRAFCDRMNSWAQGEGQPGLGYIFWREDEEGGAGPIAKNIGPERASGIRDQLGLKVG
                     DACFFVAGDPAKFYKFAGIARDRIGRELNLVDENRYEFAWIVDFPFYEWNEEEKCIEF
                     GHNPFSMPQGGSAALDAATTVEELLKIKAYQYDLVCNGYELASGGIRNHRPDTMIKAF
                     GIVGLSEEDVKKRFGALYRAFQYGAPPHGGMAAGLERMVMLLTGVKTVRDVAIFPMNQ
                     QATDLLMGAPTEATPKQLRELHIRLALPERKV"
BASE COUNT          379 a          638 c          684 g          379 t
ORIGIN      
        1 tcaaatggct gaagacaagg tgagcagcac ttcgggcgga gtcgctggcg gagctgccgg
       61 tgagcccccg atgcaccgct atcgtacgca cacctgcggc gcactccgta aaggcgacgt
      121 ggggcggatc gcgcggctgt cgggctgggt gcaccgggtg cgcgaccacg ggggcgtgtt
      181 gttcatcgac ctgcgcgacc actatggcat cacccagatc gtggcggacc cggacagcaa
      241 ggccttcaag gcggccgaga cggtgcgtgc cgagtgggtg attcgcgtcg atggccttgt
      301 tcgcgaacgg ccggagggca cggtcaatcc ggatctggcg acgggcgaga tagaggttta
      361 cgcgcacgag atcgaaattc tctccgaggc caaggagctg ccggtcccgg ttttcgggga
      421 gcccgactat ccggaggacc tgcgccttac ctaccgcttc ctcgacctgc ggcgcgaaac
      481 gctgcacaat atcatcatga agcggttgga gatcacgcgg tcgatccgcc gtcggatgca
      541 cgaggccggc ttcaacgagt tcccgacacc gattcttacg gcttcgagcc ccgagggggc
      601 gcgcgacttc ctggtgccca gccgccttca tcccggcaag ttctacgcgc tgccgcaggc
      661 gccgcagcag tacaagcagc tgctgatgat ttcgggcttc gatcggtact tccagatcgc
      721 gccctgcttc cgtgacgagg acccacgcgc cgaccgcttg ccgggtgagt tctaccagct
      781 cgacgtggag atgagcttcg tcgagcagga ggacgtgttc cagaccatgg agccgatcat
      841 ccgcggcatc ttcgaggagt tcggcgaggg caagccggtt acgcccgtgt ttccgcgcat
      901 tccttacgag gaggcgatcc gcaagtacgg cacggacaag cccgacctgc gcaaccccat
      961 cgtcatgcag gacgtgacgg agcatttccg cggatcaggc ttcaaggtct tcgccaacat
     1021 gatcgaaaag gaccccaaag tccgcgtctg ggcaattccg gcgccgggcg gcggcagccg
     1081 agccttttgc gaccgcatga attcctgggc gcaaggcgaa ggccagccgg gcctcggcta
     1141 catcttctgg cgcgaagacg aggaaggcgg cgccggcccc atcgccaaga acatcggacc
     1201 ggagcgggcg agcggaatcc gcgatcagct ggggctgaag gtgggcgatg cctgcttctt
     1261 cgtggccggt gaccccgcga aattctacaa gttcgctggc atcgcccggg accgtatcgg
     1321 tcgcgagctg aacctggttg acgagaaccg ctacgagttc gcgtggattg tcgatttccc
     1381 gttctatgag tggaacgagg aggagaagtg catcgagttc gggcacaacc cgttctcgat
     1441 gcctcagggc ggcagcgcgg ctctcgatgc ggcaaccacc gttgaagagc tgctcaagat
     1501 caaagcctac cagtacgatc ttgtttgcaa cggctatgag ctggcctccg gcggcatccg
     1561 caaccaccgt cccgacacga tgatcaaggc cttcggcatc gtggggctca gcgaggagga
     1621 cgtaaagaag cgctttggcg ccctctatcg tgccttccag tacggcgcgc ctccgcacgg
     1681 cggtatggcg gcgggtctcg agcgcatggt gatgttgctg actggggtta aaacggtgcg
     1741 cgacgtcgct atctttccga tgaaccagca ggccaccgat ctgctcatgg gtgccccgac
     1801 cgaggcgacg ccgaagcagc tgcgggagct gcacattcgg ctggccctgc cggagcggaa
     1861 ggtgtaggcc gcgcttctcg agcgtagtcg gcctgcagcg ctcttaccaa cgcgctgccg
     1921 ggctcgtggt gtctgcttta tcgcgcgcag gatatgctgc aggtccggtg gtgacctcac
     1981 gcgctcatat cgcgttatgc ggacagccga tgtgtgctat cctgtactta tgaacgtgca
     2041 cgcaccgcca cgcatgactg ttgagcagtt catcgactgg
//
LOCUS       DASUMG010000355         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_368427, whole genome shotgun sequence.
ACCESSION   DASUMG010000355 DASUMG010000000
VERSION     DASUMG010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368427"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..1151
                     /gene="denD"
                     /locus_tag="VFX46_05850"
     CDS             177..1151
                     /gene="denD"
                     /locus_tag="VFX46_05850"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HEX5600137.1"
                     /translation="MKVLIIGGAGMIGQKLAQRLARDGSLAGRPITSVVLHDVVEAQR
                     CRDCPFPMTVKVGDLSRPGEADELVADKPDVIFHLAAVVSGEAELDFDKGYRINLDGT
                     RYLLEAVRKAGNKPRVVFTSSIAVFGAPFPEAIGDEFFTTPLTSYGTQKAIGELLLAD
                     YTRKGFLDGIGIRLPTICVRPGKPNKAASGFFSSIIREPLAGQEAVLPVSEDVRHWHA
                     SPRAAVGFLLHAAALDGETVGPRRNLTMPGVSVTVGEQIEALRKVAGDRVVARIRREP
                     DPLIMQIVSGWPRNFDARRAIELGFRADSSFEEIIRIHIEDELGGTIA"
     gene            1626..2423
                     /locus_tag="VFX46_05855"
     CDS             1626..2423
                     /locus_tag="VFX46_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX5600138.1"
                     /translation="MPNGSDSALGLTRDAATASDAQPEKPSARAPWQGRYPSNGQANG
                     VRSNGSGLVPGELKAQGDTPTFRDRARTVLPVEYQGREVVFEAHAYEDDSASYQALAL
                     VYRGTSPSSADELPLVRVHSGCATGDIFHSLRCDCHAQLQKAIGRVIKSRIGAIIYLP
                     HHEGRGIGLVAKIKAYAEQDRGLDTVDANLSIGAPVDARDYTLAAKILKDLGLTEIRL
                     LTNNPLKVEALQRLGIKVAEQIQHATSPSRYNARYLATKRERMAHKL"
     gene            2581..2928
                     /locus_tag="VFX46_05860"
     CDS             2581..2928
                     /locus_tag="VFX46_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX5600139.1"
                     /translation="MTITRHEPTEILSNVVEANGFVFTAGVTAKDTSQDAKGQTEEIL
                     KEIDRLLSLCGTDKSKIVSTTIWVSDIRYREPMNEVWRAWTGGKDLPGRACVEAKLAD
                     PQHLVEIAVIAAK"
     gene            2987..3433
                     /locus_tag="VFX46_05865"
     CDS             2987..3433
                     /locus_tag="VFX46_05865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX5600140.1"
                     /translation="MSKSQRRPPARRAQFKHFNLIPTRWMDNDVYGHVNNVVYYSYFD
                     TVVNRYLIDSGGLDYHSGETIGVVVETSCRFHRSFCFPEDIEAGLRVAHLGNSSVRYE
                     IGLFGVGEDEARADGHFVHVFVDRATRRPTPMPDRMRIALARLADG"
BASE COUNT          720 a         1028 c         1112 g          722 t
ORIGIN      
        1 gcgtggctgt tcaagctgat cgatgaaacc ggtttcgatg gctggatcgg ctgcgagtac
       61 agaccacggc gcggcactgt cgaaggattg aaatgggtcg agaggtgcgg tgtccgcctc
      121 gactgaaccc cacctggaag ctcgaccaca aacgacctca agggaggagc gacgtcatga
      181 aagtcctgat catcggcggt gccggcatga tcggccagaa attggcgcaa cgcttggcgc
      241 gggatggcag cctcgccggc cgaccgatca ccagcgtcgt gttgcacgat gtcgtcgagg
      301 ctcagcgttg ccgcgactgt ccatttccga tgaccgtcaa ggtgggtgac ctctcccggc
      361 caggggaggc cgatgagctg gtggccgaca agccggacgt gatcttccat ctcgccgctg
      421 ttgtctcggg cgaagctgag ctggatttcg acaagggcta tcgcattaat ctagacggca
      481 cgcgatatct gctcgaagct gttcgcaagg ccggcaataa gccacgcgtc gtgttcacca
      541 gctccatcgc tgtgttcggt gccccgtttc ccgaggcgat cggcgatgag ttctttacga
      601 cgccgctcac ttcctacggg acgcagaaag ccatcggcga gctgctgctg gcggattaca
      661 cgcgtaaagg atttctcgac ggcatcggca ttcggcttcc gacgatttgc gtcaggccgg
      721 gcaagcccaa caaggccgcc tcgggcttct tctcgagcat catccgcgag ccgttggctg
      781 ggcaggaggc cgtgcttccc gtgtccgagg acgtgcgcca ttggcacgca agtccgcggg
      841 ccgctgtcgg ctttctgttg catgctgcag cgctcgacgg cgagaccgtt ggccccaggc
      901 gcaacctgac gatgccgggc gtgtccgtga ccgtcggcga gcagatcgag gccctacgca
      961 aagtcgctgg cgacagggtc gtggcgcgta ttcgccgtga gccggacccg ctgatcatgc
     1021 agatcgtgtc cggatggccg cgcaatttcg acgcgcggcg ggctatcgag ctaggcttcc
     1081 gagcggattc gagcttcgag gagatcatcc gcatacatat cgaggacgag ctcggtggga
     1141 ccatagcata attgtgaagt gctgcgaggc cggcggtgcg ggcgcccctg gtaaggctgt
     1201 tccccgaccc ccatttgttg aaagatgcga aagcactaga acagttggct aacatcacgt
     1261 gcgaggcgca caccagcttt acgcctccga cagacagacg aggagctcat ccgtgccagg
     1321 cgggatgagg cattcagtat gcagacgagg ggtgattcac cctattggcg ccacagtgtt
     1381 cggcgctaac gttctgtcga tcacgcgagg agtgactgcg cgatggcgac atttgagtca
     1441 cgctccgaag tgttcgtcgc gttccgtcgt ggtgagcgaa gagccattcc tgcggggatc
     1501 gggttcgtga ttctcgggag gtgcgcagct ggctccgatc gagaaagcag caccgacgat
     1561 cacgtcgtcg ctctaacaac tgcctcgagc agattcgcta cgagatgatt catcgagggc
     1621 acactatgcc gaacggttca gactcagcgc tgggattgac gcgggacgcg gcgacagctt
     1681 ccgatgcgca gccggaaaag ccatcggcac gagctccctg gcagggacgc tatccatcga
     1741 acggccaggc caatggcgtg cggtcgaacg gctccggttt ggttcccggc gagctgaagg
     1801 cgcaaggcga taccccgacg ttccgggacc gggcacggac cgtgcttccg gtcgagtatc
     1861 agggccgcga ggttgttttc gaggcccatg cctatgagga cgattcggca tcctatcagg
     1921 cgctggccct tgtttatcgt gggacatcac cgtcgagcgc ggacgagctg ccgctcgtgc
     1981 gagtgcacag tggttgcgcg acgggtgaca tctttcattc tctccgctgc gactgtcatg
     2041 cccagctcca aaaagcgata ggacgcgtca tcaaaagccg gattggggcg atcatctatc
     2101 tgcctcatca tgaggggcgc ggtatcggcc tcgttgcgaa gataaaggct tatgcggagc
     2161 aggaccgagg cctcgacacc gtggatgcga acctttcgat cggcgcaccc gtcgatgccc
     2221 gggattatac gcttgccgcg aagatcctta aggacctggg cctcaccgag atcaggctgc
     2281 tgacgaacaa tcctctcaaa gttgaggcgc ttcagcggct cggaatcaag gtcgccgagc
     2341 agattcagca cgccacatca ccatcccgct acaacgcgcg ctatctcgcg accaagcggg
     2401 aaagaatggc gcacaagctg tgatcagcgg ttgaaagtcc gcgcagctcg aacgtccggc
     2461 cattcaaggt cgggagattg cttctcggac agcacagcta tagtacccgg agtgctggca
     2521 gaagctgcct agcgtggtag gagttgcgtt ggtccaacgt aatcgaatgg ggagaaacgc
     2581 atgaccatca cacgtcacga accaacggag atcctatcca atgtggtgga ggcgaacggc
     2641 ttcgtgttta cagccggagt taccgccaaa gatacaagcc aggacgcgaa gggccagacc
     2701 gaggagattc tcaaagagat cgatagactg ttgagtttgt gcggtactga caagagcaag
     2761 atcgtctcta caaccatttg ggtgtcggat attcgttacc gcgagccgat gaatgaagtc
     2821 tggcgcgctt ggactggtgg caaggacctt ccggggcgcg cttgcgtcga agcgaagctc
     2881 gcggaccctc agcatttggt tgaaatcgct gtgattgccg cgaagtagat tgcactcgat
     2941 gggccggatt acatcaatgt tttccggccc tactccagaa gacctcatga gcaaaagcca
     3001 acgcagacct ccggcccggc gcgcacaatt caagcatttc aacctgatac ctacgcgctg
     3061 gatggacaac gacgtctacg gccacgtcaa taacgtggtg tactactcat attttgatac
     3121 ggttgttaat cggtatctga tcgattcagg cgggctcgat tatcattcgg gtgaaaccat
     3181 cggcgtcgtc gtcgaaacca gttgccgctt tcaccgctcc ttctgctttc ccgaggacat
     3241 tgaagccggt ttgcgcgtcg cccatctcgg caactcgtcc gttcgttacg agatagggct
     3301 gttcggcgtc ggcgaggacg aggctcgcgc cgatggccat ttcgtccatg tgtttgtcga
     3361 tcgggcgaca cgtcggccga caccgatgcc cgatcggatg cggatcgcat tagcgcgtct
     3421 cgctgacggt tgaggcgtcg gctgccttgc cagtcggcgt aggccggtgc ggcccctgaa
     3481 tgctgacccg gcattttttc tgcccgaaac gatcagttta ctgcgtatat tgaagcccac
     3541 cgatgcttcg acggcgtttg cgcaattaga catatcaccc tg
//
LOCUS       DASUMG010000356         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1090417, whole genome shotgun sequence.
ACCESSION   DASUMG010000356 DASUMG010000000
VERSION     DASUMG010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090417"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..328
                     /locus_tag="VFX46_05870"
     CDS             26..328
                     /locus_tag="VFX46_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX5600141.1"
                     /translation="MAIDEKTRIELEASAFRKLVAHLRERTDVQNIDLMNLAGFCRNC
                     LANWYQEAANAAGIPMTKEEAREIIYGMPYEEWRAKYQKEATPEQKAAFEKAHRHS"
     gene            477..767
                     /locus_tag="VFX46_05875"
     CDS             477..767
                     /locus_tag="VFX46_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEX5600142.1"
                     /translation="MNGLQASAQQQLRQLVEQLERLEEEKKAIAEDIREKFAEAKALG
                     FDVKVLRQVLRLRKKSKADRMEEEAILATYLHALGMLDPEPETESAKMMAAE"
     gene            complement(968..1825)
                     /locus_tag="VFX46_05880"
     CDS             complement(968..1825)
                     /locus_tag="VFX46_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX5600143.1"
                     /translation="MLKRIIEIRRVGSAVMVLALCLIVSACASSNDAAKMLNPDPPEK
                     MYAEADQLLSRGKFEAAAKKFEDLDRDHPYSPEARKAMVMAAYAYYRAGKTQEAIATA
                     RRYTTMHPGTKEAALAHHIIISSYFDEMVGPNRDQTNARKALAELQTLKARYPDSPYA
                     KDADNRIRIAQDMIAAHEMEVGRYYLKRKNYLAAINRFKTVVTDYQTTAHVEEALMRL
                     VEAYMALGIKQEAQTAAAVLGHNFPNSPWYKDAYALLQSDGLAPREDTGSWISRAWRN
                     FKLPKLTSG"
     gene            complement(2100..>2298)
                     /locus_tag="VFX46_05885"
     CDS             complement(2100..>2298)
                     /locus_tag="VFX46_05885"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX5600144.1"
                     /translation="VGDLALAGYPLLGAYRSVRGGHRLNASVLKALFADGSAWTLVEA
                     PRVREPFVRFPTPVAAVAAAE"
BASE COUNT          453 a          682 c          710 g          453 t
ORIGIN      
        1 ggatagctcg atcgggagaa cggttatggc gatcgacgag aagaccagga ttgagctcga
       61 agcttcggcc ttccgcaagc ttgtcgcgca cctgcgcgag cgtacggatg tccagaatat
      121 cgacctgatg aaccttgccg ggttctgccg caactgcctc gccaactggt atcaggaggc
      181 ggctaatgct gccggcatcc ccatgaccaa ggaggaggcg cgcgaaatca tctacggcat
      241 gccttacgag gagtggcgcg ccaagtatca gaaggaagca acgcccgagc agaaggcggc
      301 gttcgagaag gcgcacaggc actcttgagg ggcgagggtg agttgcgtgg ataagttccg
      361 cgtttgcaac ctcttcgcct tgctgtgatc tcttccccgc gatagcgatg ggcgcacgta
      421 tgcggtcggg aaatgagagc cactagcccg gcgcgctcca agacggggga tgtcaaatga
      481 atgggctcca agcctcggcg caacaacagc tgcgccagct cgtcgagcag ctcgagcggc
      541 tcgaggagga gaagaaggcc attgcggaag acattcgtga aaagttcgcc gaggcaaaag
      601 cgctcggttt tgacgtcaag gtgctacgtc aggtgctccg ccttcgtaag aagagcaagg
      661 cggaccggat ggaggaagaa gctattctgg cgacctatct ccacgctctc ggcatgcttg
      721 atccagagcc cgagaccgaa agcgcgaaga tgatggccgc tgaataatga attgcgcgtt
      781 gcggattgtt ccgatcgccc gttgagcaac gcgcattcga ctgagcttaa cccccacctc
      841 cgagattacc cgtccgcttc attcgctgga tgacagcgtc acgacctaag ctaccacgca
      901 cagtgcactc tgtgcgaatc gcctgcgtgc gtcgtcgcgg ctcagctcac caatacgtct
      961 tgcctactta accgctggtc agcttgggca gcttgaagtt ccgccacgcg cggctgatcc
     1021 acgagccggt gtcctcccgc ggcgcgaggc cgtcggactg cagaagcgca tacgcgtcct
     1081 tgtaccaagg cgagttcggg aagttgtggc ccaacacagc tgcggccgtc tgcgcctcct
     1141 gcttgatgcc gagggccata taggcttcca cgaggcgcat gagcgcttct tccacgtgcg
     1201 cggtcgtctg ataatcggtg acgacggtct taaagcgatt gatcgcggcg agatagttct
     1261 tccgcttcaa gtaatagcgc ccaacctcca tttcgtgggc tgcgatcatg tcctgggcga
     1321 tgcgaatacg attgtcggca tccttcgcgt atgggctgtc gggataacga gctttcagcg
     1381 tttgcagctc ggccagcgct ttgcgcgcgt tcgtttggtc ccggttcgga ccgaccatct
     1441 cgtcgaagta ggacgagatg atgatgtggt gcgcaagcgc ggcttccttt gtgccggggt
     1501 gcatggtcgt gtagcgccgg gcagtcgcaa tggcttcttg tgtcttaccg gcgcgataat
     1561 aggcgtaggc cgccatgacc atggctttgc gcgcctcggg ggagtaggga tggtcccggt
     1621 caagatcctc gaattttttg gccgcagctt cgaacttgcc gcgggacagc aattgatccg
     1681 cctcggcgta cattttctcc ggcggatcgg gattgagcat ctttgcggcg tcgttggacg
     1741 atgcgcaggc gcttacgatg aggcaaagcg ccaagaccat caccgcagat ccgacccgcc
     1801 gaatttcgat tattcgtttc agcaccacct agcccccagc tctgcgctgt cgcagccgcc
     1861 tcccgatgcg taggctgccg cgcaggtcaa catcggttcg accagcgtct caccggttgc
     1921 gcatagtatg cgattcgatt cgcccgtgac ggcaagccgc tgagcgaaag atcgttatcc
     1981 ccctacttta aagcggccat ttgtggtcgg gatggggcaa gttccgaccc gtctcagcct
     2041 gtccgatcag gccgctggag gcctagcgtg cggttaccac tgagagtcgg aggtgcagct
     2101 cactccgctg ctgcgaccgc cgccactgga gtgggaaagc gaacgaaggg ctcgcgcacc
     2161 ctcggcgcct cgaccagcgt ccaggccgag ccatcagcga acagagcctt cagcacgctc
     2221 gcattcaagc gatgaccacc gcgcaccgag cggtaggcac ccaacagcgg atagcctgcc
     2281 agagcgaggt caccgacg
//
LOCUS       DASUMG010000357         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_46927, whole genome shotgun sequence.
ACCESSION   DASUMG010000357 DASUMG010000000
VERSION     DASUMG010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46927"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1922)
                     /locus_tag="VFX46_05890"
     CDS             complement(30..1922)
                     /locus_tag="VFX46_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5600145.1"
                     /translation="MARGTESAATAHNGAARKKAAGTVSPMPRAVSQRESARTDEKPL
                     IEPRPTIHDVDTSALFGEALDQSLNYLTSRFTLGLSPAALTEAYFDWLIHLAWAPGKR
                     LQLTERAVRKMIRLARYAALCMTKPEGVPPCIEPAPDDPRFVGEDWHRWPFNVIHQAF
                     LLTQQWWKIATTDVRGVTKQHEHEVEFATRQILDVFSPSNFLLTNPVVLRKTAEEGGQ
                     NLVRGFWNFVEDWQRLINGQPPPGMEDFKVGETLALTPGKVVYRNRLIELIQYEPATD
                     KVRPEPVLIVPAWIMKYYILDLRPGKSLVEYLTKQGFTVFMISWKNPTAEDRDLSFED
                     YRRLGVLEAVKAVKTIVPDVGIHGVGYCLGGTLLAIAAAALARSHQNSFKTLTFFAAQ
                     VDFEEAGELMLFINESQLVFLEDMMKMQGFLGNYQMAGAFQLLRSNDLIWSRVVRDYL
                     MGERQPMFDLMAWNADTTRMPYRMHSEYLRKLFLENDLAAGRYVADGRPIALTDIRVP
                     VFAVGTETDHVAPWRSVYKVHLLLDTEVTFVLTNGGHNAGVVSEPGHPRRRYRVSTKE
                     EDDLYVDADTWFEQTPVKQGSWWPEWAAWLSERSGEWTSPPPMGAPEKGYRPILDAPG
                     TYVLQP"
     gene            complement(2240..2470)
                     /locus_tag="VFX46_05895"
     CDS             complement(2240..2470)
                     /locus_tag="VFX46_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX5600146.1"
                     /translation="MSEESSYPTGGERREGIDRRLLELLVCPLTKTPLEYDEARQELI
                     SRAARLAFPIKEGVPLMTVEAARHLDDEEQRS"
     gene            complement(2485..>2737)
                     /locus_tag="VFX46_05900"
     CDS             complement(2485..>2737)
                     /locus_tag="VFX46_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S16"
                     /protein_id="HEX5600147.1"
                     /translation="RPNLLRVLKNYLEAHKLKADWRAILSAPSEYLVNSLSVVCPYGA
                     EEKQALLEAKDLKTRAEVLMALAEMELASSESGSGGAVN"
BASE COUNT          542 a          827 c          804 g          564 t
ORIGIN      
        1 atcgcgcgca tcggtcggcc ttcgatcgtt caaggttgca gaacgtaggt tccgggggca
       61 tcgaggatcg gcctatagcc cttttcgggc gcgcccattg ggggtggaga agtccactcg
      121 cctgagcgct ccgagagcca agccgcccac tcaggccacc aggagccctg cttcaccgga
      181 gtttgctcga accaggtgtc cgcatcaacg tagaggtcat cctcctcctt ggtgctgacc
      241 cggtagcggc gacggggatg gcccggctcg gacacgacgc cggcattgtg gccaccattc
      301 gtgagcacga aggtcacctc cgtgtcgagc aggaggtgta ccttgtagac ggaccgccac
      361 ggcgcgacgt ggtcggtctc cgttcccaca gcaaacactg gtacgcggat gtccgtcagc
      421 gcgatcggcc ggccgtccgc cacatagcgc ccagccgcga gatcattctc gaggaacagc
      481 ttccgcagat actccgaatg catgcggtag ggcattcgcg tcgtatccgc gttccacgcc
      541 atgagatcga acatgggctg ccgctcgccc atcaggtagt cgcgaacgac ccgcgaccag
      601 atgaggtcgt tggaacgcaa gagctggaag gctccggcca tctgatagtt tccgaggaag
      661 ccctgcattt tcatcatgtc ctcgaggaac acgagctggc tttcgttgat gaacagcatc
      721 agctcgcccg cttcctcgaa atcgacctgt gcagcgaaga acgtcagcgt tttgaaggag
      781 ttttgatgcg atcgtgcgag agcagcagct gcgatggcca gcagcgttcc gccgaggcaa
      841 tagcccacac cgtgaatgcc cacatcggga acaatcgtct ttacggcctt aactgcctcc
      901 agcacgccaa ggcgacggta atcttcgaaa ctcaagtcgc ggtcctcggc ggtcggattt
      961 ttccaggaaa tcatgaagac cgtaaagccc tgcttcgtga gatactcgac gagcgatttt
     1021 cccggcctca aatccaaaat gtaatatttc atgatccagg cgggcacgat gagcacgggc
     1081 tccggccgca ccttgtccgt ggccggctcg tattggatca gctcaatcag acggttgcgg
     1141 tagacaacct tgccaggggt gagcgccagc gtctcaccaa ccttgaaatc ctccatcccc
     1201 ggaggaggct gcccgttaat cagccgctgc cagtcctcga caaaattcca aaaaccccga
     1261 accaggtttt gcccgccttc ctcagcggtt ttgcgcagca cgacagggtt ggtcaggagg
     1321 aaatttgaag gtgagaagac gtcgagaatc tgccgtgtag cgaattcgac ctcgtgctca
     1381 tgctgcttgg tgacgccgcg tacgtccgtc gtcgcaattt tccaccattg ctgtgtgagc
     1441 aggaaagcct gatggatgac gttgaatggc cagcggtgcc aatcctcgcc cacgaagcgt
     1501 ggatcgtcgg gtgccggctc gatacagggt ggcacacctt ccggtttcgt catgcacaat
     1561 gccgcataac gggcgagccg gatcatcttc cgtacggccc tctccgtcag ctgcaatcgc
     1621 ttacctgggg cccaggcaag gtggatgagc cagtcgaaat aagcttcggt gagcgccgcc
     1681 ggcgataggc caagcgtgaa ccgcgaggtg aggtagttga gcgactgatc aagcgcctcg
     1741 ccgaagagcg ccgaagtatc gacgtcgtgt attgttggcc gaggctcgat gagaggtttc
     1801 tcgtccgttc gcgcgctttc ccgctgagaa actgccctcg gcatggggct cacggtgcca
     1861 gctgctttct tacgagcagc tccgttgtgg gcagttgcag ccgattccgt tccgcgtgcc
     1921 attcgtcaaa tcctctttgt tcgatgatat gccgcggcgc ggccggaacg tctttgacgg
     1981 aagtcatgcc tgtgtttggt cccccatacg gcttccgccc ccaatcctag ggaaaaacgc
     2041 tcacctggcg gtttggttca gcaattttcg gggctatttc gaattgcggc gatcgctcca
     2101 tgtgatggga aacgctgtaa taacgagcat tactgaatgg ccgcgatgag ccgttgcgcc
     2161 acatccgata cgaaactgga aaccggcgag agaaactcgg cttgcgtggt tcagcagcta
     2221 ggcatcactg cagaggaagc taagaccttt gctcctcgtc atccaggtgg cgcgccgctt
     2281 ctacggtcat cagtggcacg ccttctttga tgggaaacgc gaggcgcgcg gcgcggctga
     2341 tcagctcctg gcgggcttca tcatactcca acggcgtttt ggtaagcggg cacaccagaa
     2401 gctccagaag gcggcggtcg atgccttctc tccgctcgcc accggttggg taagaagact
     2461 cttccgacat ggtcgtttcc aaactcagtt cacagcgccg cccgagccgc tctcgctcga
     2521 tgccagctcc atctcagcaa gagccatgag cacctcggca cgggtcttca ggtccttggc
     2581 ctcgaggagc gcctgcttct cttccgcacc ataagggcac accaccgaga gggagttgac
     2641 caggtattcg ctcggcgcgc tcaggatcgc ccgccagtcc gctttcagct tgtgagcctc
     2701 gagatagttc ttcagcacgc gaagcagatt gggccgg
//
LOCUS       DASUMG010000358         4336 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_528923, whole genome shotgun sequence.
ACCESSION   DASUMG010000358 DASUMG010000000
VERSION     DASUMG010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528923"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VFX46_05905"
     CDS             complement(<1..567)
                     /locus_tag="VFX46_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5600148.1"
                     /translation="MNLYLPIAEMPVNIFVFLGMGGAVGFLSGLFGIGGGFLMTPLLI
                     FNGIPAAVAVGTEAAQIVASSVSGAIAQWRRNNVDIKMGTVLLTGGIAGSLVGVEAVK
                     LLRQIGQFEFFVSVAYVTFLGIIGVLMLMESANAIRKSRQGKAPAIKRPGQHLWMHKL
                     PLKMRFHRSKLYISAIPPFAIGMVVGLLG"
     gene            complement(775..1128)
                     /locus_tag="VFX46_05910"
     CDS             complement(775..1128)
                     /locus_tag="VFX46_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX5600149.1"
                     /translation="MVVCKRIYEPSSPKDGYRVLIDRLWPRGVKREEAAIKEWIKELA
                     PSNELRRLATADESEWSEFQRRYRMELSKPEVQPTLARLREIARSGTLTLLYAKRDTQ
                     HNNAVVLKEVLEEGL"
     gene            complement(1187..1600)
                     /locus_tag="VFX46_05915"
     CDS             complement(1187..1600)
                     /locus_tag="VFX46_05915"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5600150.1"
                     /translation="MAWYKSAAEKGHLKAMHNFAVLKASENSPDFETAAHWFGKAANS
                     GLVDSQYNLAILYEIGRGVERDLKEAYKWFALAARAGDKEAARRRDRVRWMLTAAELE
                     AAETMIRSWEPEPVDRAINDPRIAGEAWKRNEDRG"
     gene            complement(1625..3832)
                     /locus_tag="VFX46_05920"
     CDS             complement(1625..3832)
                     /locus_tag="VFX46_05920"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600151.1"
                     /translation="MSYNKKRSSTDDAYRASRTTRPHVRERGADELRGLLQRIADHIA
                     AADRRQQSALGDVQSRKDRFSENWPSQGAPTEAAGRTFWSDPLQEELRRRASTAAHSQ
                     MFADRVTGSRAHTRHQHPRYEDQHEGWDPASAEALTRLYEPEHTAGPHTIFSDLTSFD
                     APKSGQENWAQDELGSATYPSSNHRPEGVDRLESRIADLAMRIEQALAQLRSGSAAEA
                     LDARLASLDSKLGTALQGLPTRADVEQLQALEGHIRELAQYVERAHHRLTRLDDIEMH
                     LARLVEQTSDEHLIQLLDQQIHSESHVSRLAEAVAEHLSDRDSFRDELGSGANRLREL
                     HELIEQFVSTQRQEQQTASALEGIQQSVLKLLDRMEALEQTYNSVRTADDAEAGELGA
                     AHRDDGTKSRPRYPLKPVDPYAPSVVTISRVTKDSPPMSAGSKAENTGAEASGLPAMG
                     ELSGQGSEAFSAASHAPGSAEQAPLPRTREAFLNAARRAAQKASSKPVGGESLAPRKR
                     AALGGRRPSGTGPLSGFLVAALALVLAVGVGVTTYSAYKDEFGNAWKLGQRVLELGSS
                     GRGSAAGGRSGSASPTRDGMGAGGQSPDAPGHTPRLDSGEVAEEARAVTSSPGGIVIA
                     PSVITASAHAGEVPVTAALTAPDADTVSGQPINRTAADNKPMPPAGVGPLSLRIAAAN
                     GDPSAEFEVAVRFDEGRGVPRDHVEAAKWYKRSAAKGFAPAQYRLGTLYERGL"
BASE COUNT          799 a         1373 c         1284 g          880 t
ORIGIN      
        1 gcccaagagg cctaccacca tgccgatggc gaatggcggt atagcgctga tgtacagctt
       61 cgaccgatgg aagcgcatct tcagcggcag cttgtgcatc cagagatgct ggcccggacg
      121 cttgatggca ggggccttgc cctggcgact cttgcggatt gcattggcgc tttccatcag
      181 catcaacacg ccgatgatgc cgaggaaggt cacgtaggcg accgaaacga aaaactcgaa
      241 ctgaccaatc tgacggagga gcttgaccgc ctcaacgccg acgagagatc cggcaatacc
      301 tcccgtcaac agcacggtgc ccatctttat gtcgacgttg ttgcgtcgcc actgagcaat
      361 agcaccggaa acggaggagg cgacgatctg ggctgcctcg gtgccgactg cgacggcagc
      421 cggaataccg ttaaaaatga gcagcggcgt catcagaaag ccgccaccaa tgccgaacag
      481 accagagagg aagccaaccg caccacccat gccaaggaac acgaaaatgt tcacgggcat
      541 ttcggcgatg ggcaggtaca gattcatgac agcgccgcaa cctgatccgg catcgcgact
      601 cctgggccgg cgccgctcgt gcgccggaga agacctgctc agccggaaga ggcagaattt
      661 aggtgagtaa ccggtcctgt cctttaagcc agcctacgcg ctctgggtca atctacaatt
      721 cgctcagcaa accggaacgt ttcaattttt gtaacagaat aagtccggtg tgagctaaag
      781 accttcctcc agaacctctt tcagcaccac ggcattattg tgctgcgtat cgcgtttcgc
      841 gtagagcaac gtcagtgtgc ccgatcgggc gatctcacgc agtcgtgcga gcgtcggctg
      901 aacctctggt ttcgacagct ccatccggta gcgccgctga aactcggacc attcgctttc
      961 gtcagcagtg gcgagccggc gcagctcgtt gctcggcgcc aattctttga tccattcctt
     1021 gatcgccgcc tcctcgcgct tgacaccgcg cggccacagg cgatcgatca acacgcgata
     1081 gccatccttt gggcttgaag gctcatagat acgcttacag acgaccacgg cgctcgcctc
     1141 agggcaaatg gggaccaagc cgtgtttcgc gacatgcgtg tcgcgatcag ccccgatcct
     1201 cgttacgctt ccacgcctct ccggcaatcc tgggatcgtt gatcgcacgg tcgactggtt
     1261 cgggctccca ggagcggatc atagtctcgg cggcctcgag ctcagcggca gtgagcatcc
     1321 acctcacgcg atcccgccgc cgggctgcct ccttgtcacc cgcccgtgcg gcaagggcga
     1381 accatttgta ggcctccttg aggtcacgct ctacgccacg accgatttca tacaggatcg
     1441 ccagattgta ttgactgtca accagcccgc tattcgcagc cttaccgaac cagtgagcgg
     1501 ccgtttcgaa gtccggtgaa ttttcgctgg ccttgagcac ggcgaaattg tgcatggctt
     1561 tcagatgccc cttttccgcg gcgctcttgt accaggccat ggcccggccg cggtccgccg
     1621 ccagtcacag accacgttca tagagtgtac cgagacggta ttgggccggc gcaaagccct
     1681 tcgctgcgga gcgcttgtac catttggccg cttccacgtg atctctggga acaccgcgcc
     1741 cttcgtcgaa gcgcaccgcg acttcgaact cggcagacgg gtcgccgttg gcggcagcaa
     1801 ttcggagcga aagcggtccg acgcctgcag ggggcattgg tttgttgtcc gccgccgtgc
     1861 ggttgatggg ctggccagaa acggtatcgg catctggcgc cgtcagggcg gctgttacgg
     1921 gcacctctcc cgcatgagcg ctcgcagtaa tcacacttgg ggcaatgacg atgccgcccg
     1981 gtgacgaggt aaccgcgcgt gcctcttcgg caacctcccc cgaatcgaga cgaggcgtat
     2041 gccccggagc gtccgggctt tgcccccctg cacccatccc gtcacgcgtg gggcttgcac
     2101 ttcccgaacg tccgccggcc gcgctgcctc gaccggatga gcccagctcc agaactcttt
     2161 ggcccagttt ccaagcgttg ccgaattcgt ctttgtaggc gctgtaggtt gtgaccccga
     2221 caccgacagc caacaccagc gccagcgccg cgaccaaaaa gcctgacaac ggacctgtgc
     2281 cggacgggcg acgcccacct aatgcggccc gcttccgtgg cgccaagctc tcaccaccaa
     2341 ccggcttgct cgaggccttt tgggctgcgc gacgggctgc attgaggaac gcctctcggg
     2401 tcctaggaag cggcgcttgt tcagcgctcc cgggcgcgtg cgatgctgca ctgaaagctt
     2461 cagagccctg tcccgacaac tcgcccattg ccggcaaacc ggaagcttcc gctcctgtgt
     2521 tttcggcctt gctgccagcg ctcatcggcg gcgaatcctt ggtcacccgg ctgatcgtga
     2581 ccacgctcgg tgcatacgga tcgactggct tgaggggata ccttggtcgg gacttcgttc
     2641 cgtcgtcccg atgcgcggcg ccgagttccc cggcttctgc gtcgtcagca gtccggaccg
     2701 agttgtaggt ctgttcgagc gcttccatgc gatcgagcag cttcaggaca gattgctgga
     2761 tcccttccag cgcggaagct gtctgttgct cctgccgttg cgtggagacg aactgctcga
     2821 tcagctcgtg cagctcgcga agccggtttg cccctgatcc aagctcgtcg cggaagctgt
     2881 cgcgatcgga aaggtgctcg gccacagcct cagctagtct cgaaacgtgt gactcggaat
     2941 gaatctgctg atcgagcagc tggataaggt gctcatcgga ggtctgctcg acaagccgcg
     3001 ccaagtgcat ctcaatgtcg tcgaggcgag tcagacgatg atgggcccgc tcgacatact
     3061 gggccagctc cctgatgtga ccttctagcg cctgcaattg ctcgacatcg gcgcgcgttg
     3121 gaagaccttg caacgccgtg ccgagcttcg agtcgaggct cgccaatcgg gcgtcgagcg
     3181 cctcggcggc gctccccgaa cgcaactgtg ccaacgcctg ctcgatccgc atggcgaggt
     3241 ctgcgatcct cgattcgagc cggtcgacac cttcgggccg atgattcgag gagggatatg
     3301 tggcggaacc taattcgtcc tgagcccaat tctcttgccc ggatttcggc gcatcgaagc
     3361 tggtgagatc cgagaagatt gtgtgtggac cggcggtatg ctcgggctcg tagagtcgcg
     3421 tcagcgcctc cgccgacgcg ggatcccatc cctcgtgctg atcctcatac cggggatgct
     3481 ggtgcctcgt gtgtgctctg cttccagtga cgcgatcagc aaacatctgg ctgtgcgctg
     3541 ccgtggaagc gcggcggcgc agctcctcct gcagaggatc tgaccagaat gttcgacctg
     3601 ctgcttctgt tggagcgccc tgactaggcc aattctcgga aaagcggtcc tttcggctct
     3661 gaacgtcgcc cagagccgat tgctggcggc gatcggctgc ggcgatatga tcggcgatgc
     3721 gttgcagcag accccggagc tcatcagcgc cgcgttcgcg aacgtgtgga cgcgttgtcc
     3781 tcgacgcgcg gtaggcgtcg tctgtcgaac tcctcttctt gttgtatgac attccttccc
     3841 cgcgaggtcg agcttcgttg cgaatgccct cggtcgaggc cggagaaagc gggaagctca
     3901 ttctcccgcg agtcgtctgt gccgctcgag cgcatccgcc ggtccgatac gcggcacgat
     3961 ctggagccgt cttcggaacg ctatgaggtt ctagtaaaag ttcagttaac gattgttacg
     4021 acttttagta attcttcagt gtttccgaac acacttgccg caaactagcc gcataactct
     4081 gctcgatgcg ggggctcgct ccgatgtcga tggtgcgtct tcttcccggc gagcttgaag
     4141 cggacgctac ccgaggcgac atcaacaggt catcgacggc ttcctcatcg gacagccaaa
     4201 ccttaccaat cgaagggaga tgcggatgcg cgccctactt cttgccttct ccataattgc
     4261 cgtcgtcgcc ctcggcgtca ccggggttgc gagccaggaa aagcagggcc gttatgtcat
     4321 gagcccgacg gagaac
//
LOCUS       DASUMG010000359         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_930602, whole genome shotgun sequence.
ACCESSION   DASUMG010000359 DASUMG010000000
VERSION     DASUMG010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930602"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            299..1405
                     /locus_tag="VFX46_05925"
     CDS             299..1405
                     /locus_tag="VFX46_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5600152.1"
                     /translation="MKIKRRQFLAGVATAGAAAGTATFPKPAIAQARTEWRMVTTWPK
                     NFPGQGVGAERFAKAVTEATEGRLVIKVYAAGEIVPAFESIDAVIRGAADLMHATPYY
                     WPNKSKALNFFSTVPFGMTTPELDAWMNHLGGQELWDKLYDQFGLKAFHAGSTGVQMG
                     GWFNKEITGVDSIKGMKMRIPGIGGEALAKLGMTIINLPAGEVFAALQSGTIDATEWV
                     GPYNDLALGLYKVAKYYYWPGMHEPGTQIEVTVDKKKFTALPKDVQRIFEACCRAENN
                     MLTSEFNARNGAALETLIREHKVQLKRFPNDFLQAYGTAAGEVIQEMRDSGDPLTKEV
                     VESFLKARRDLMNWARIADQGFMNARMLDYKFPS"
     gene            complement(1465..1674)
                     /locus_tag="VFX46_05930"
     CDS             complement(1465..1674)
                     /locus_tag="VFX46_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600153.1"
                     /translation="MNDLTILEIGVALLVIWLATSLALWKLIDRQSRPGPVKNALAKE
                     SLMLIHLALLVAGLSLTIKGLQIFS"
     gene            complement(1693..>2884)
                     /locus_tag="VFX46_05935"
     CDS             complement(1693..>2884)
                     /locus_tag="VFX46_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEX5600154.1"
                     /translation="IIVGALLAASTGVVGATVITMGLISLPAMLRAGYCPKMATGAIG
                     ASSTLAQIIPPSTVLIFIGDLLAGVNQSAQLRLGNFAPDPISVGDLFAGALIPGFILV
                     GLFLLWTIYKAVFQPETVPAILMTPEERKGLPVRVVAVLIPPLILIVAVLGSILFGFA
                     TPTESASVGAIGAMLLAAANRKLSFSTIRYAALNTMITTSIIFTIVLAASLFSLVFRG
                     LGGEHMVEQALHNLPGGAFGAMLAVMAIMFILGFFLDTFEIILIMLPICGPPLILLGL
                     DPLWIGVMVAMNLQTSFMTPPFGFTLFYLRGVAPPKVATAQIWAGAFPFVVLQLMGLA
                     LLWSFPSTATWLPKKLFGPAAIEQSAPAGASTGGFGEFIQDDSFQLDDEGMPLEVDDP
                     FSKR"
BASE COUNT          661 a          825 c          892 g          506 t
ORIGIN      
        1 tccgttgatt ggcagcaaga attggccatt cggctccagt ttatgtgcag cgcaacaaat
       61 atcgcgattt ttcgctgctc actgcagata gttaaacgaa ctaggagatc aaggtccagg
      121 tcgaatccgg aagttaggtg cagctgcgtc aaagagcgcc ttccgctccc ggatcaatac
      181 gctcccgcgg acatatgaac tgcgttgcct aacgaccgcg acgaacacat actcggcgat
      241 acggctcaca ccagccgaaa aacaatctga aatggggcag cccaagggag caaatcgtat
      301 gaagatcaaa cgccgccagt ttcttgcggg cgtggcgacc gctggcgcag ccgctggcac
      361 ggcaacgttt cccaaacccg ccattgcgca ggccagaacc gaatggcgca tggtgaccac
      421 atggccgaag aactttcctg gccagggcgt gggtgccgag cgcttcgcga aagccgtcac
      481 cgaagcaacc gaaggccggc tggttatcaa ggtttacgca gcgggcgaga tcgtcccggc
      541 ctttgaaagc atcgatgctg tgatccgcgg cgctgctgac ctgatgcacg caacgcccta
      601 ttactggccc aacaagagca aggcgctgaa cttcttctca acagttcctt tcggcatgac
      661 aacgccggag ctcgatgcct ggatgaacca ccttggtggg caggagctgt gggacaagct
      721 ttatgatcag ttcggcctca aggcatttca tgccggctcg accggcgtgc agatgggagg
      781 ctggttcaac aaggagatca ccggcgtcga ctcgatcaaa ggcatgaaga tgcgcattcc
      841 gggcatcggc ggcgaagcgc tggccaagct cggcatgacg atcatcaacc tgccggccgg
      901 cgaggttttt gcggcgctgc aatcgggcac catcgatgcc accgagtggg tcggcccgta
      961 caacgatctg gcgctcggtc tctataaggt tgccaagtac tactactggc cgggtatgca
     1021 cgagcctggc acgcagatcg aggtgaccgt cgacaagaag aagttcacgg cgctgcccaa
     1081 ggatgtgcag cggatcttcg aggcctgctg tcgggccgag aacaacatgc tgacctcgga
     1141 gttcaacgcc cgcaacggtg ctgcactgga gacgctcatc agagagcaca aggtgcagct
     1201 caagcgcttc ccgaatgact tcctgcaggc ctatggcacc gcggctgggg aggtcattca
     1261 ggagatgcgc gacagcggag atccgctcac gaaggaggtt gtcgagtcct tcctgaaggc
     1321 tcggcgtgac ctgatgaact gggcgcggat cgccgaccag ggcttcatga atgcccgaat
     1381 gttggactac aagttcccgt cgtaatcgcg cctaggaagg aacaagcgac ggggcccgcc
     1441 agggccccgt tttgctattg gccgtcagct gaagatttgc aggcctttga tggtcagaga
     1501 gagtccagca acaagcaaag ccagatggat aagcatcagg ctttccttgg cgagcgcgtt
     1561 cttgacggga cctggccgac tctgacgatc gatgagcttc cacagggcga gcgaggtcgc
     1621 aagccaaata acaagtaacg cgactccaat ttcgaggatt gtcaggtcgt tcatggcaat
     1681 tgcctcgccc gctcaccgct tcgagaaggg atcgtcgacc tcgagcggca ttccctcatc
     1741 gtcgagctga aaactgtcgt cctggatgaa ctcgccgaag ccgccggtgc tcgctcctgc
     1801 gggggcggat tgctcgatcg ctgctggccc gaagagcttc ttgggcagcc aggttgctgt
     1861 gctcggaaat gaccaaagca aagcaagtcc catgagctgc agcaccacga acggaaaggc
     1921 tccagcccag atctgggcgg tggcaacttt cggcggtgcg acgcctcgaa ggtagaacag
     1981 cgtgaaccca aagggaggcg tcatgaagct cgtctgcagg ttcatggcga ccatcacacc
     2041 gatccagagc ggatcaaggc cgagcaggat cagcggcggt ccgcagatcg gcagcatgat
     2101 gaggatgatc tcgaacgtat cgaggaaaaa gccgaggatg aacatgatcg ccattacggc
     2161 gagcatcgct ccgaaggcgc cgccaggcag attgtgcaag gcctgctcca ccatgtgctc
     2221 gccgccgagg ccgcggaaaa ccagcgagaa aagcgaagcc gcgagcacga tcgtgaagat
     2281 gatcgacgtc gtgatcatag tgttgagggc ggcataccgg atggtggaaa aagatagctt
     2341 tcgattggcc gccgccagca gcatggcacc gatggcgccg acagaagccg actccgttgg
     2401 cgtcgcgaag ccaaacaata tgcttcccag caccgctaca attaggatca gcggtggaat
     2461 gaggaccgcc acgacgcgga ccggcaaccc cttgcgctcc tccggggtca tcaggatggc
     2521 gggtaccgtt tcgggctgga atacggcttt gtagatcgtc cagagcagaa agagcccgac
     2581 gagtatgaac cctgggatca gagcgcccgc gaaaagatcg ccgaccgata ttgggtccgg
     2641 cgcgaaattg ccgagcctca gctgcgccga ttgattaacc ccggccagca gatccccgat
     2701 aaaaatcaat accgtggagg gcgggatgat ttgcgcgagc gtcgacgacg cgccgatcgc
     2761 tccggtggcc attttggggc agtagccagc ccgcaacatg gccggcaggc taatgaggcc
     2821 catggtgatg acggtcgcgc cgacgactcc ggtcgaagcc gcaagcaggg ctccgacgat
     2881 gatg
//
LOCUS       DASUMG010000360         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_208122, whole genome shotgun sequence.
ACCESSION   DASUMG010000360 DASUMG010000000
VERSION     DASUMG010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208122"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1097)
                     /locus_tag="VFX46_05940"
     CDS             complement(123..1097)
                     /locus_tag="VFX46_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600155.1"
                     /translation="MRFLGVGLIALALMLVPLSTEAASPQPARGDGPFATHEQILQWI
                     NDYRLEPEPKKLPEALKAMSRLGLLRDQDTTGVYVGFAAGVLGSNPNMAEKLVTRMFP
                     MPPEDQAVIVKAIAYSGLPDWKRLLGKFVERMPARKALIDSYLFGKAPLLHELPLETG
                     PAPVDTLWGYYFATGSLEPVYRIVEALKWSDDKSDVNRLTIGSVAKWTLANNASRDKE
                     LLDFLRQQQHMTNHPEKVRKALQEIVEAAETFETTKIRKDALAAIEELKRTGPQPNNP
                     WSKAAYYGSTAIALGCVVASALGATAIGLPCILTGAASSAAVKWLNGP"
     gene            1319..>2253
                     /locus_tag="VFX46_05945"
     CDS             1319..>2253
                     /locus_tag="VFX46_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX5600156.1"
                     /translation="MAPHQNEHVGPDPTDDQPSPAVPTDGARRSAAEATPSIAQYLEI
                     KAANPDSLLWYRMGDFYELFFEDAVVASQALGIVLTKRGKHLGEDIPMCGVPVVRADE
                     YLQRLIKQGYRVAVCEQLEDPAEARKRGSKAIVRRDVVRLVTPGTLTEDTLLDAKARN
                     YLTALHIAPQGAETDGPIIALASLDISTGEFELGSVTLNDLPGELTRLQPSEIVAADT
                     SLARSDIKSWIDHIGAAATPVPAATFDSMAGEETLKTRLGVAELAAFGNFSRPELAAA
                     AALLKYVELTQIGRQPIIRPPRSTGPANTMVIDAAT"
BASE COUNT          418 a          740 c          673 g          422 t
ORIGIN      
        1 agcgggcgcc cttttgaggc gcccgctttc ctttagggat ggccggatca ggtggaggat
       61 gctggctcag gagccatctc ccctttagct cccaacaacg aatttcctaa aagccccatt
      121 cctcacggcc cgttcagcca cttcaccgcg gccgaagatg ccgcgcccgt caaaatgcac
      181 ggcagcccga tagctgtggc ccccaatgcg ctggcaacca cacatcccag agcgattgcc
      241 gtgctgccat agtaggcggc cttcgaccag ggattgttgg gctgcgggcc ggtgcgcttc
      301 agctcctcga tggcggcaag cgcgtccttg cggatcttgg tggtctcgaa agtctccgcc
      361 gcttcgacga tctcctgcag agcttttcgc accttctccg gatgatttgt catgtgctgt
      421 tgctggcgca ggaaatcgag cagctccttg tcgcgagagg cgttattggc aagtgtccat
      481 ttcgcgacgc tgccaatggt cagtcgattg acatcgctct tgtcgtccga ccacttcaac
      541 gcctcgacga tacggtacac cggctcgagt gagcctgtgg cgaagtagta gccccagagc
      601 gtgtcaaccg gggccggtcc ggtctcgagc ggcagctcgt gcaggagagg cgccttgcca
      661 aacaggtagc tgtcgatcag cgccttgcgt gccggcatcc gctcgacgaa cttgccgagc
      721 aaacgtttcc agtcgggcaa gccgctatag gcaatcgcct tgacgatgac tgcttgatcc
      781 tccggcggca tcgggaacat tcgggtcacg agcttttccg ccatgttcgg attggacccc
      841 aatacgcccg ccgcgaaacc gacgtagact cccgtcgtat cctggtctct cagcagcccc
      901 aggcgactca tcgccttgag cgcctcgggt agctttttcg gctcgggctc caggcggtaa
      961 tcgttgatcc actgcaggat ttgctcgtgc gtcgcgaacg gaccgtctcc ccgagcgggc
     1021 tgcggactcg cggcctcggt agacaacggc acgagcatca gagcgagtgc gatcaggccg
     1081 acgccaagga aacgcatcac atcctccgaa aaactgatac acgcacgccc tcccgcaact
     1141 tggtccgcac acaccctgca agcgttaagc aatagcacgc gtcgatttca tggcggcatc
     1201 agcaactcat gtgcgcgagt tagacctggg cacgttgtta gcaacatcgg tccgactttg
     1261 acctgctgac gcggggcgca tatgtggttc ccggaacgaa agggggaccg caacgccgat
     1321 ggcgccgcat cagaacgagc acgtcggacc agatccaacg gacgatcagc cctcgccagc
     1381 cgttcccacg gatggcgcgc gccgttccgc ggcagaagcc acgccctcca ttgcgcaata
     1441 tctggagatc aaggctgcca acccggacag cttgctctgg taccggatgg gcgacttcta
     1501 tgagctgttc ttcgaggatg ctgtcgtcgc ctcccaggcg ctcggaatcg tgctcaccaa
     1561 gcgcggcaag cacctcggag aggatattcc gatgtgcggc gtgcccgtcg tgcgagccga
     1621 cgaatatctg cagcgcctca tcaagcaggg ctatcgcgtt gctgtctgcg agcagctcga
     1681 ggatccggcg gaggcgcgga agcgcggctc gaaggcgatc gtacggcggg acgtggtccg
     1741 gctcgtcacg ccaggcacgc tgaccgagga tacgctgctc gacgccaagg cccgcaacta
     1801 tttgaccgcg ttgcatatcg ccccgcaggg cgccgaaacg gacgggccga tcatcgcgct
     1861 cgcttctctc gatatctcca cgggcgagtt cgagctgggc tccgtcacgc tgaacgatct
     1921 gcccggggag cttacccgcc tgcagcccag cgagatcgtc gctgctgaca cgtccctcgc
     1981 ccgctctgac atcaaatcct ggatcgatca catcggtgca gctgcaacgc ccgttcccgc
     2041 tgccactttc gacagcatgg ctggcgaaga gaccctcaag acccggctgg gcgtggcgga
     2101 gctggcggct ttcggcaact tctcacggcc agagcttgcg gccgctgcag cgcttctgaa
     2161 gtacgtggag ctgacccaga ttgggcggca acccatcatc cgtccaccgc gcagcaccgg
     2221 gccggccaac accatggtca tagacgcggc gac
//
LOCUS       DASUMG010000361         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1090664, whole genome shotgun sequence.
ACCESSION   DASUMG010000361 DASUMG010000000
VERSION     DASUMG010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090664"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="VFX46_05950"
     CDS             complement(<1..136)
                     /locus_tag="VFX46_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1150 family protein"
                     /protein_id="HEX5600157.1"
                     /translation="MTNDKQIDPNTTHPAITQEALAQLGDGRLAYVKTIRSEDVANLF
                     P"
     gene            complement(197..622)
                     /locus_tag="VFX46_05955"
     CDS             complement(197..622)
                     /locus_tag="VFX46_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX5600158.1"
                     /translation="MSRVPSLSSPFLLGFDEIERVLDRVAKGADGYPPYNIERLPRDG
                     QNPERLRITLAVAGFTLDQLDVSIEESQLVIRGRQQDDKTRQYLHRGIAARQFQRTFV
                     LCDGMEVMGADLKNGLLSIDLIRPQPERIVKTISINEQE"
     gene            796..1905
                     /locus_tag="VFX46_05960"
     CDS             796..1905
                     /locus_tag="VFX46_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX5600159.1"
                     /translation="MTNEPRLPLMTIRALRGTPVEVPLNFVLGTSAVAVHKAPLLLVD
                     LETEEGVTGRTYLFCYVPAAAPAIISLLTEVERLVKGETVAPGELWKKLARRFTLIGV
                     QGIVRMAMSAFDTAAWDALAIAADKPLANFLGARVRPIPAYNSCGLGLKDDLGELADE
                     AEKLLVSGFGAIKLRLGYPTLGADIAAVHAVRKRIGDNVALMVDYNQALTVEDALDRG
                     RALDVEDIYWLEEPIRHDDYVGAARLARELNVPIQIGENFSLPAAMATALEQHAADYV
                     MPDLERIGGVTGWRAAAELARTHNLKMSSHLFPEVSCHLLAATPTCHFIEYVDWANIL
                     LEEPLVLADGHAQTPDRPGSGIVWDKKAIERYRMR"
     gene            complement(1907..>2428)
                     /locus_tag="VFX46_05965"
     CDS             complement(1907..>2428)
                     /locus_tag="VFX46_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX5600160.1"
                     /translation="EALDVPFYLHPRNPLPSWARSYEGHNWLLGPNWAFAAETAVHAL
                     RLIGSGLFDECPNLKIVLGHIGEGIPVYLWRIDNRNGWMKAPHKYPAKHGVAHYFRKH
                     FHLTTSGNFDTASLVNAISVMGVDRVMFSVDWPFEDIGEGAQWMDAAEISDADRAMVG
                     RTNAIKLFKLPIR"
BASE COUNT          494 a          658 c          725 g          551 t
ORIGIN      
        1 gcgggaacag attcgcgacg tcctcggaac ggatcgtctt cacataagcg agacggccgt
       61 cgccgagctg tgccagtgcc tcctgcgtga tcgcgggatg agttgtgttc gggtcaatct
      121 gtttgtcgtt ggtcatggcc ctcgactcct ttctgattca aggcttgacg cggtcgagtc
      181 ctgcttcacg agtccgtcat tcctgctcat tgatcgagat ggttttcacg atccgttccg
      241 gttgtggccg gatcaaatcg atcgacaaca ggccattttt caagtcggcg cccataacct
      301 ccattccatc acagagcaca aatgtgcgct ggaattggcg tgcggcgatg ccgcgatgga
      361 ggtactggcg agtcttgtca tcctgttgcc gccctcggat caccaattgg ctttcctcga
      421 tgctcacatc gagctgatca agggtaaatc cggcaacggc cagcgtgatg cgcagccgtt
      481 cgggattttg cccgtcacgc ggtagccgct cgatgttata gggcgggtag ccgtcggcgc
      541 ccttggcaac gcgatcgagc acgcgctcga tctcatcgaa tcccaagagg aacgggctgg
      601 agagggacgg aactcgcgac attggtctca aagccctcgc ttgaagcgac tttgacgggg
      661 cccctttcag gcaccccaac cgaaaagcga ctgccttccg gtcgttccta atatgggcgc
      721 gctgggttaa aggttcaaga acggggccga atccgcccca gaagaatcct ttgtgggcgt
      781 ttcagacggt ttttcatgac caatgaaccc agacttccct taatgaccat ccgtgctttg
      841 cgcgggacgc cggtagaggt tccactcaat ttcgtgctcg ggaccagcgc ggtcgccgta
      901 cataaggctc ctcttttgtt ggtcgacctg gaaactgagg aaggggttac gggccgcacg
      961 tatctcttct gctacgtgcc ggcagctgcg ccggcaatta tcagcctgct taccgaagtt
     1021 gagcgactcg tgaagggtga aaccgtcgcg ccgggcgagc tatggaagaa acttgcacgc
     1081 cgcttcaccc ttatcggggt gcagggcatc gtgcgaatgg cgatgtcggc ttttgatacg
     1141 gcggcctggg acgcgcttgc catcgctgcg gataagccgc ttgcgaattt cctcggggcg
     1201 cgggtgcggc ccattcccgc ttacaacagc tgcggccttg gactgaaaga cgatttgggc
     1261 gagctcgctg atgaggcgga aaaactgctc gtgagcggat tcggcgcaat caagcttcga
     1321 ttaggttatc cgacgctggg ggctgacatt gcggcggttc atgccgtacg aaagcgtatc
     1381 ggcgacaatg ttgcgctgat ggttgactac aatcaggcgc tgacggttga agatgcgctt
     1441 gaccgaggtc gcgcgttgga tgtcgaagac atctactggc ttgaggagcc gatccgtcac
     1501 gacgattatg tgggcgctgc caggttggcg cgcgagctca acgtgccgat ccagatcggc
     1561 gagaactttt cgctcccggc tgccatggcg acagccttag aacagcacgc ggcggactat
     1621 gtaatgcccg atctggaaag gatcggcggc gtaaccggat ggcgtgcggc ggccgagttg
     1681 gcacgaacgc acaatttaaa aatgtcgtcg catctctttc ccgaagtcag ctgtcatctt
     1741 cttgctgcaa cgccgacatg ccattttatc gaatatgttg attgggcaaa tattcttctt
     1801 gaagaaccgt tggtcctcgc agatgggcac gcgcaaactc cggaccgtcc cggcagtgga
     1861 atagtctggg ataaaaaggc gatcgaacgt tatcgtatgc gttgagctaa cgaatcggta
     1921 gtttgaagag cttgatcgcg tttgtgcggc cgaccatggc tcgatctgca tcgctgattt
     1981 cagcggcgtc catccactgg gcaccttctc caatgtcttc gaacggccag tcgaccgaaa
     2041 acatgacacg atctacgccc atcacagaaa tggcattgac tagcgaagct gtgtcgaagt
     2101 tgcctgaggt cgttaggtga aaatgtttgc ggaagtaatg agccactccg tgcttggctg
     2161 gatatttatg tggagccttc atccagccgt tgcggttgtc gatgcgccag aggtacacgg
     2221 gaatgccttc gccaatatgg ccaagcacga tcttcagatt tgggcattca tcaaacaagc
     2281 cgctgccgat cagccgaagc gcgtgtaccg cggtttctgc ggcgaatgcc caatttgggc
     2341 cgagcagcca gttgtgccct tcgtatgatc tagcccaact cggaagcgga ttgcgcggat
     2401 gcagatagaa gggcacgtcg agagcttc
//
LOCUS       DASUMG010000362         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_208197, whole genome shotgun sequence.
ACCESSION   DASUMG010000362 DASUMG010000000
VERSION     DASUMG010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208197"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFX46_05970"
     CDS             complement(<1..296)
                     /locus_tag="VFX46_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX5600161.1"
                     /translation="MRVAALIVAAGRGTRAAAASIPKQYRMLGGQPVLARTIRALAAD
                     ARIETIQVVIHPDDRALYDACCSTLDAPLREPVHGGATRQESVRAGLNALADVNP"
     gene            472..1488
                     /gene="dusB"
                     /locus_tag="VFX46_05975"
     CDS             472..1488
                     /gene="dusB"
                     /locus_tag="VFX46_05975"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX5600162.1"
                     /translation="MRPTQRCPNQLSIGSLQLKNPVVLAPMSGVTDLPFRSLALRLGA
                     GLVVSEMVASEELVQQRPEALRRAVGIGTSPFVIQLAGREARWMAEGARIAEDLGADV
                     IDINMGCPARRVTGKLSGSALMRDLKHALSLIEAVVRNTRRPVTLKMRMGWDHNHLNA
                     PELARLAEQVGVQLVTVHGRTRCQFFEGRADWAFVRRVKEAVSIPVIVNGDIRTVEDA
                     REALRLSGADGVMVGRGAYGAPWLPGRIATALRTDADPGPPSLAEQGRIAVSHIIDML
                     FHHGRSHGLRRARKHIGWYLEQSGLSFDRLKFWRSRLCGDDDPRRVLRGMAEFYDRAE
                     EPTA"
     gene            1485..2618
                     /locus_tag="VFX46_05980"
     CDS             1485..2618
                     /locus_tag="VFX46_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5600163.1"
                     /translation="MSAEATPERSLTAGGPRHIQHDLLLAALPHPVLALADGERIVYA
                     NPAAEQFFSAGQATLKRHRFADLVAFGCPLVNLVEKVRRTGASVNEYGIEVVIPRVEG
                     TRLVDAHGTPLPEQPSLIVLMMQQRTMAQMIERQLTHRAAARSVSGLAAMLAHEIKNP
                     LSGIRGAAQLLEPNLSPSDRALAQLICAETDRIRSLVDRMEVFGDERPLAKEPVNIHD
                     VLDHVWRLASAGFGRGVEFVLRFDPSLPPVPGNRDKLVQAFLNLVKNAVEAIEEGGES
                     GRIILTTAFRPGMRLSVPGSGSRVSLPLMIEVEDNGPGVSEHVKPHLFDPFVTTKRSG
                     TGLGLALVAKIIGDHGGIIECESEPRRTIFRVLLPMQDDRPKT"
     gene            2635..>2804
                     /locus_tag="VFX46_05985"
     CDS             2635..>2804
                     /locus_tag="VFX46_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5600164.1"
                     /translation="MARGTILIADDDSAIRTVLNQALARAGYIPRATSYAATLWRWVS
                     QGEGDVVITDVI"
BASE COUNT          487 a          878 c          919 g          520 t
ORIGIN      
        1 ggattgacat cggccagtgc attgagtcca gcccggaccg actcctggcg cgtggcgccg
       61 ccgtgaactg gctcgcggag cggcgcatcc agcgtcgagc agcaggcatc gtagagggcg
      121 cggtcatcgg gatgaatgac aacctgaatc gtctcgattc gggcatccgc cgcgagcgcc
      181 ctgatggtcc gcgccagaac cggttggccg ccaagcatcc gatattgttt cgggatggat
      241 gccgctgcgg cgcgtgtgcc gcgtcctgca gcgacgatta gcgcggccac gcgcacttcg
      301 atcctcccgt gccttcaacc gcatcacgaa tgctggcgtg aagctctaat cgccgtatgc
      361 actcgatgca acggtggcgt gcgacgttgc aattcgcact tgcgaaacac gctctcctgc
      421 cctagtctgc ccaaacattc agcacaggat tgaagtgacc aatatctagg catgagaccc
      481 acccagcgtt gcccaaacca gctcagcatt gggtctcttc agcttaagaa cccagtcgtg
      541 ctcgctccga tgtccggagt gaccgatctg ccgttccgca gcctggccct tcggcttggt
      601 gccgggctcg ttgtttccga gatggtagcc agcgaggagc tagtacagca acgcccggag
      661 gcattgcgca gggccgtggg aataggcacc tcgccattcg tcattcagct tgcggggcgc
      721 gaggctcgat ggatggcaga gggtgcccgc atcgcggagg acctcggcgc cgacgtcatc
      781 gacatcaaca tgggctgccc ggcccggaga gtgacgggga agctttcagg ttcggcgctg
      841 atgcgcgacc tcaagcacgc gctgtctctc atagaggctg tcgtcaggaa tacgcgccgg
      901 ccggttacgc tcaagatgcg gatgggctgg gatcataacc acctcaacgc gccggagctg
      961 gctcgattgg ccgagcaggt cggtgtgcaa ctcgtcacgg tccatggccg cacacgttgc
     1021 cagttcttcg aggggcgtgc cgactgggcg ttcgtccggc gcgtgaagga agcggtttcc
     1081 atcccggtta tcgtcaacgg cgatattcgg accgtcgagg acgcccgaga ggcgttgagg
     1141 ctttcggggg ccgatggggt catggtcgga cgcggtgctt atggcgcacc gtggctgccg
     1201 ggacgcatcg cgacggcgct tcggacggac gccgatcccg gcccgcccag cttggccgag
     1261 cagggccgca tagccgtctc gcacatcatc gacatgctct tccaccacgg ccgttcgcat
     1321 gggctgcgtc gggcccggaa gcacatcggc tggtatctgg agcagagcgg actttcgttt
     1381 gatcgcctca agttctggcg cagccggttg tgcggcgacg acgacccccg gcgcgtgctt
     1441 cgcggcatgg ccgagtttta cgaccgcgca gaggaaccta ctgcatgagt gcagaagcaa
     1501 ctccggagcg atcactgact gctggtgggc cgcggcatat tcaacatgac ctgctgttgg
     1561 cggcgctccc tcatccagtt ttggcgctcg ccgacggcga gcggatcgtc tacgccaatc
     1621 cggcggcaga gcagtttttc tcggcgggtc aggcgacctt gaagcggcac cggttcgccg
     1681 atctggtcgc cttcggatgc ccgctggtca atttggtcga aaaggttcgc cgcacgggcg
     1741 cttcggtcaa cgagtacggg attgaggttg tcatcccgcg ggtcgaagga acgcggttgg
     1801 tggatgccca cggcaccccg ttaccggagc agccgtcgct catcgtgctg atgatgcagc
     1861 agcgcacgat ggcgcagatg atcgagcggc aactcacgca tcgcgcggcc gcgaggtcgg
     1921 tctctggctt agcggccatg ctcgcccacg agatcaagaa cccattgtcc ggcattcggg
     1981 gagcggcgca gctgctggag ccgaacctta gtccttccga tcgggcgctg gcacaactca
     2041 tctgtgccga gacggatcgc attcgcagcc tcgtcgatcg gatggaggtg ttcggggacg
     2101 agcgcccgct ggcgaaggag ccggtgaaca tccatgacgt gctggatcac gtctggcgtc
     2161 tcgcatctgc tggattcgga cgcggtgtcg aattcgttct tcgattcgat ccgtcgctgc
     2221 cacccgtgcc gggcaatcgc gacaagctgg tccaggcctt cctcaacctg gtgaagaatg
     2281 cggtcgaggc gatcgaggag ggcggcgaga gcggtcgcat catcctgacg acggcattcc
     2341 gccccggcat gcgcctgtcc gttccgggca gtggctcgcg ggtgagcctg cccttgatga
     2401 tcgaggtgga ggacaacggg ccgggcgtat ccgagcacgt caagcctcat ctgttcgatc
     2461 cgttcgtcac gaccaagcgc agcggcaccg ggctcgggct tgctctggtc gccaagatca
     2521 ttggcgacca cggtggcatc atcgagtgcg agtcggagcc gcggcggacg atcttccgtg
     2581 tcctgctccc catgcaggac gaccgaccca agacctagag agatgggcta acccatggca
     2641 cgcggcacga tcctgatcgc cgacgacgac agcgccattc ggacggtgct caaccaggcg
     2701 ctcgctcgcg ccggctacat cccacgcgca acgagctatg cggcaacgct ctggcgatgg
     2761 gtctcccaag gcgaaggtga cgtcgtcatc accgacgtca tcat
//
LOCUS       DASUMG010000363         2550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_288531, whole genome shotgun sequence.
ACCESSION   DASUMG010000363 DASUMG010000000
VERSION     DASUMG010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288531"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VFX46_05990"
     CDS             complement(<1..603)
                     /locus_tag="VFX46_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5600165.1"
                     /translation="MNDRTGRARKIIDASESYTAADIEVLEGLEPVRRRPGMYIGGTD
                     ERALHHLFAEVIDNAMDEAVAGHADRIEVRLDADGFVTVTDNGRGIPVDPHPKFKNKS
                     ALEVIMTTLHSGGKFENKVYNTSGGLHGVGVSVVNALSETLEVEVARGQQLYRMVFSR
                     GEPQGKLQKLGSIMNRRGTKVRFKPDEQIFGKGVTFKPARL"
     gene            complement(832..1686)
                     /gene="panC"
                     /locus_tag="VFX46_05995"
     CDS             complement(832..1686)
                     /gene="panC"
                     /locus_tag="VFX46_05995"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX5600166.1"
                     /translation="MSRGQVKVVRSIAELREVVRGWRQGGKRIGLVPTMGALHEGHLA
                     LVRTANAMSDRTVVSIFVNPTQFAPNEDFARYPRDEAGDLEKLSAIGCDLVWMPDVAE
                     MYPAGFATSIVPKGPAEGLESDHRPNFFTGVATVCTKLFTQVTPDLAVFGEKDYQQLC
                     VVRQVVRDLDLPIEIVAHPTVREPDGLAMSSRNTYMSPAERSRAPLIYQTLVRAARDI
                     AEGSSIAAATSRGREELTAAGFKVDYFEVRDARTLGVVEDAAKRPLRVLAAAWLGETR
                     LIDNVPLE"
     gene            1891..>2550
                     /locus_tag="VFX46_06000"
     CDS             1891..>2550
                     /locus_tag="VFX46_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX5600167.1"
                     /translation="MAHQPVAARSSPDQSPVIPPRDEIAAYHLIEEPRLVGALIERAI
                     FGEDERRRIAELARRLVHAARAGRHKFSGIDAFMHEYGLTSEEGVILMCLAEALLRIP
                     DAETADKLIAEKIGGGHWEKHLGRSDSLFVNASTWGLMLTGQIVKLRDVHGEGPANLL
                     KRMVLRSGEPVIRQALRHAMRILGNQFVLGRTIDEALDRAESYQEKGYLFSFDMLGEA
                     AK"
BASE COUNT          428 a          826 c          831 g          465 t
ORIGIN      
        1 cagccgcgcc ggcttgaagg tcacgccctt cccgaagatc tgctcgtccg gcttgaagcg
       61 caccttggtg ccgcgccggt tcatgatcga gccgagcttc tgcagcttgc cctgcggctc
      121 gccgcggctg aacaccatgc gatagagctg ctgaccgcgc gcgacctcga cctccagggt
      181 ctcagagagt gcgttgacga ctgacacgcc cacgccgtgg aggccgcccg aggtgttata
      241 gaccttgttc tcgaatttgc cgcccgagtg cagggtggtc atgatgacct cgagggcgga
      301 cttgttcttg aacttggggt gcgggtcgac ggggataccg cggccgttgt ccgtcaccgt
      361 cacgaagccg tccgcatcga ggcgcacctc gatccggtca gcatggccag ccacagcctc
      421 gtccatcgcg ttgtcgatga cctcggcgaa caggtgatgc agcgcgcgct catcggtgcc
      481 gccgatgtac atgccagggc gacggcggac cggctcgagg ccctcgagca cctcgatgtc
      541 cgcggccgta tagctctccg acgcgtcgat gattttgcgg gcgcgccccg tcctgtcgtt
      601 catttccttc tcaagctttg cgaacagatc gcgttgagac tgagccatgc gcggtgttcc
      661 tcgctcccag gtggacgagc agcacaacgc cacgagcgtt cccggccccc tgccggcgaa
      721 tctcacgcaa aatacagagg cgtccctcgc atatggcagc gaagctgaca agcagctgcg
      781 gggtcacgcc gtgcgcctca cgagaggacc gtggcaagct tgcgcttacg cctactccaa
      841 ggggacgttg tcgatgagac gtgtttcgcc aagccaggct gcggcgagca cacgcagcgg
      901 ccgtttcgct gcgtcctcga ccacgccgag ggttctggca tcccgcactt cgaaatagtc
      961 gaccttgaag cccgctgccg taagctcttc ccggcctcgg ctcgtggctg cggcaataga
     1021 ggagccttcg gcgatgtcac gggccgctct aacaagtgtc tggtaaatga ggggtgcgcg
     1081 agaccgctct gctgggctca tataggtatt tcgcgagctc atcgcgaggc cgtccggttc
     1141 gcggacggtc gggtgggcga cgatctcgat gggtaggtcg aggtcgcgga cgacctgccg
     1201 cacgacgcag agctgctggt agtccttctc gccgaagacc gcgagatccg gagtgacctg
     1261 cgtgaacagc ttcgtgcaga ccgtggcgac accggtgaag aagttcgggc ggtggtcgct
     1321 ttccaggcct tccgccggcc ctttcggcac gatgctggtg gcgaagcccg cggggtacat
     1381 ctccgccacg tcgggcatcc acacgagatc gcaaccgatc gccgacagct tctcgaggtc
     1441 cccagcctcg tcgcgggggt agcgagcaaa gtcctcgttc ggcgcgaact gcgtggggtt
     1501 gacgaagatg gagacgaccg tgcggtcgct catcgcgttg gccgtccgga ccaaggccag
     1561 atgcccctcg tgaagcgcac ccattgtcgg cacgagaccg atgcgcttcc cgccctgacg
     1621 ccagccgcgc acgacctcgc gcagctcggc gatggagcga acgaccttga cctgtcctcg
     1681 cgacacagct caccccgttc gtgtggcgga tgttgcggtg cgaaagtcgt tcgctccgcc
     1741 ggtttcgtca aggagcattg tggccaacgc gcccgatcgc caaatgtgag gaagccgcgg
     1801 tctcttcttg ttgaatgaga ttggtccagg atcggaccgc gggatctgct acgatgcgtg
     1861 cggcttgatt cagccccctc cgagattgcc atggcccatc agcctgttgc cgcccgatcc
     1921 agccccgacc aatcgccggt catcccgccg cgcgacgaga ttgcggctta tcacctcatc
     1981 gaggagcccc ggctcgtcgg ggcgctcatc gagcgtgcga tcttcgggga ggacgagcgg
     2041 cgacgcatag ctgagcttgc acggcgtctt gtccacgccg cgcgtgccgg acggcacaag
     2101 ttctccggca tcgatgcctt catgcacgaa tacgggctga ccagcgagga aggcgtcatc
     2161 ctgatgtgcc tcgcggaggc cctgctgcgg atcccggacg ctgagacagc cgacaagctc
     2221 atcgccgaga agatcggcgg tggccactgg gagaagcatc tgggccgctc ggacagcctg
     2281 ttcgtcaacg cctcgacatg gggcctgatg ctgacggggc agatcgtcaa gctccgcgat
     2341 gtccacggcg aggggccggc gaacctgctc aagcgcatgg tgctgcgctc gggcgagccg
     2401 gtcatccggc aggctctgcg ccatgccatg cggatcctgg gcaatcagtt cgtgctgggc
     2461 cgtaccatcg atgaggcgct ggacagggcc gagtcgtacc aggaaaaggg ctatctgttc
     2521 tccttcgaca tgctcggcga ggcggccaag
//
LOCUS       DASUMG010000364         2273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_690002, whole genome shotgun sequence.
ACCESSION   DASUMG010000364 DASUMG010000000
VERSION     DASUMG010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690002"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /locus_tag="VFX46_06005"
     CDS             complement(<1..1062)
                     /locus_tag="VFX46_06005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5600168.1"
                     /translation="MLPELTHSGTLRVFMTADAVGGVWQYALDLAQGLRAYDTETTLC
                     VLGPPPSPQQRAAAQRIPGLRLIYLDLPLDWTATCPEDVLDAAATLVEVVQDDGSAIV
                     QLNSPALAAGPRYPVPVVAVCHSCMASWWQAVRTRPMSRQFRWHKDLVAQGLEKADAI
                     VTPSAAFARTIQNVYDLPTPPKVVYNGRRPCVREPLPEGAIPDTPFAFTAGRLWDEGK
                     NLHTLDAAAARLRLPVFAAGPLKGPNRASIKLSHVRTLGNLSDGQVAEWLAAKPVFVS
                     TARYEPFGLAVLEAAEAGCALVLSDIPTFRELWDGAACFVPPDDDAAVADAIEQLAND
                     ARQRTLYASAARRRARRYTP"
     gene            complement(1047..>2273)
                     /locus_tag="VFX46_06010"
     CDS             complement(1047..>2273)
                     /locus_tag="VFX46_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HEX5600169.1"
                     /translation="APHLPLELGYTRAFLMDAGHDVLMLDGHLCGQSAEELADAVAAF
                     GPDMTVVTTAPSYLFWRCAQPELSVPREFLLALADRGGTTVAAGPHGSATPRAVLRKL
                     GVDIVVMGECEEVVADLAIGRPLSDISSIAYRSDHIVINGRPRAARFENFMPLRWPTE
                     WIARHHHHHHRFDREPDGPGAEVEASRGCPYACTFCAKMDFRDRYRRRNLNLLLEEID
                     GLIAQGVTYLYFIDEIFLPNVELLEALAARKVAFGVQTRIDLWKPELIDALGNAGCVS
                     IEAGVESLSAEGRDVLAKRCRFSTDELAERLIYARRTIPFVQANLIKMAGDDARQIAE
                     WRANLERHGVWANDPVPLYPYPSSPDYYRLWGAPDDHAWERAHAHYLAQFTELSELQD
                     ERPRPLSELEATCCQN"
BASE COUNT          442 a          690 c          730 g          411 t
ORIGIN      
        1 gggcgtgtac cggcgagcac gccggcgcgc agccgacgca tagagcgtgc gctgacgggc
       61 gtcgtttgca agctgctcga tggcatcggc gacagcagcg tcatcatcgg gcggcacgaa
      121 acatgcagct ccgtcccaca gctcgcggaa ggttgggatg tccgagagca ccagcgcgca
      181 tcccgcctcg gcggcctcca gcactgccaa tccaaatggc tcgtagcgtg ccgtcgacac
      241 gaagaccggt ttcgcagcga gccactcggc gacttggccg tcactcaagt ttccgagcgt
      301 ccgcacatgg gaaagcttga tactcgcccg gttcgggccc ttgagcgggc ccgcggcaaa
      361 caccgggagt ctcagccgcg cggccgcagc gtccaaggta tgcaagttct tgccttcgtc
      421 ccacaggcgc ccagcagtaa aggcgaaagg cgtatcggga atcgcaccct cgggaagcgg
      481 ctcacgcaca catggtctgc gtccgttgta aaccactttc gggggcgtcg gcaggtcata
      541 gacgttctgg atcgtccgag cgaaagccgc actcggcgtg acgatggcat cggctttttc
      601 caggccctgt gcgacgagat ctttgtgcca gcggaattgg cgggacatcg ggcgggtgcg
      661 gacggcctgc caccagctag ccatgcatga atggcagacg gccacgactg gcacaggata
      721 acgcggccct gccgccaggg ctgggctgtt gagttggaca atggcgcttc catcgtcctg
      781 cacgacttcc actagcgtgg cggcggcgtc gagcacgtcc tcgggacagg tcgcggtcca
      841 gtcgagcggt agatcgagat atatcagccg cagccccgga atgcgctgcg cggcagctcg
      901 ttgctggggg ctgggtggcg gaccgagcac gcataaagtt gtctcagtat cgtaggccct
      961 gagcccctgg gcgagatcta acgcgtactg ccagacgcct ccgactgcat cggcggtcat
     1021 gaaaacgcga agagtgccgg aatgtgtcag ttctggcagc atgttgcctc caattcggat
     1081 aaggggcgtg gccgctcgtc ttggagctca ctgagctctg tgaattgagc gaggtaatgg
     1141 gcgtgtgccc gctcccaggc atgatcgtcc ggcgcgcccc aaaggcggta gtaatcaggg
     1201 gagctgggat aggggtagag cggcacggga tcgttggccc aaactccgtg ccgttcgaga
     1261 ttggcgcgcc attccgcgat ttggcgcgca tcgtcgccgg ccatcttgat gaggttcgcc
     1321 tggacgaatg ggatggtgcg gcgggcgtag atgagtcgtt cggccagctc gtccgtcgaa
     1381 aaacggcagc gctttgccag cacgtcgcgg ccttccgcac tcaggctttc gacacccgct
     1441 tcgatggaga cacaccctgc gtttccgagt gcatcgatta attcgggctt ccaaaggtca
     1501 atgcgagttt gaacaccgaa cgctactttt cgcgcggcaa gcgcctcgag cagctctacg
     1561 ttgggcagga atatttcgtc gatgaaatag aggtaggtca cgccttgggc gataagccca
     1621 tcgatctctt caagcagcag gttgagattg cgccgccgat agcgatcgcg gaagtccatt
     1681 tttgcacaga atgtgcaagc atacggacaa ccacgcgacg cttcgacctc ggcccctggt
     1741 ccatcgggct ctcgatcgaa gcgatggtga tgatgatgat gccgcgcgat ccactcggtt
     1801 ggccaccgaa gcggcatgaa attttcgaac cgcgccgcac gcggccgtcc gttgatcaca
     1861 atgtggtcgg atcgataggc gatggatgaa atgtcgctga gcggacgccc gattgccaga
     1921 tcggcaacga cctcttcgca ttcgcccatc acgacgatgt ccacgccaag cttgcgtagg
     1981 acagctcttg gagtcgccga tccatgggga ccggcagcaa ccgtggtgcc acctctatcc
     2041 gccagcgcga gcagaaactc gcggggcacc gatagctccg gctgtgcaca gcgccagaaa
     2101 agatagctcg gcgccgtcgt cacgacggtc atatcggggc cgaaggccgc aaccgcatcg
     2161 gccagctcct cagcgctctg gccgcacagg tggccatcca gcatcaggac atcatgccca
     2221 gcgtccataa ggaacgcccg ggtgtagcca agttcgagcg gcaaatgcgg ggc
//
LOCUS       DASUMG010000365         2341 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1011786, whole genome shotgun sequence.
ACCESSION   DASUMG010000365 DASUMG010000000
VERSION     DASUMG010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011786"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFX46_06015"
     CDS             complement(<1..855)
                     /locus_tag="VFX46_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX5600170.1"
                     /translation="MQETPTLSPSNAPAAPVDLVPEIVQRARSAMAVYAREIAAGGQA
                     RIDEAVTAIAWSLYKPENARRMAEIAVEVTGLGNVESKVIKNQRKTFGTLRDLLRVKS
                     TGIIEEIPEKGIVKYAKPVGVVAAITPSTNPSATPVNKAMMALKGGNAIVIAPPPTAW
                     GATKPTVDLMRAELKKIGLPEDLVQVLPSPVTREATTRLMELSDLVVCTGSQANVRQA
                     YKSGKPAIGVGLGNAPVIIDASADLDDAAYKIMLSKTFDYATSCSSENAVIIVDSVFD
                     AAIAALERQ"
     gene            1032..1880
                     /locus_tag="VFX46_06020"
     CDS             1032..1880
                     /locus_tag="VFX46_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5600171.1"
                     /translation="MRGPSNGQERGSALDKALAVLEAITAQSQPIGLPDLSARLKLPR
                     QTVHRVLTHLEKLGLVVRDPSRDRFSVGPRLSTLALNALRTHNQSAPIHAILQQLVDE
                     IEETCNVGVLDGLQFVYLERIECQWPLRVHLQAGSRVPAHCTSGGKVMLAHLDEAVRA
                     RLLRNVTLKPCTKYTITRTEELEAELAEVRRRGYAVNNQEFMIGIIGVAVPIADCQGR
                     VLAALAVHGPSPRLDLDRAIGFVPRLKEASAIMADAWTSGPEVADTPTSSRASSARTA
                     PARVGD"
     gene            1983..>2341
                     /locus_tag="VFX46_06025"
     CDS             1983..>2341
                     /locus_tag="VFX46_06025"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX5600172.1"
                     /translation="MTRMTASEAFVETLVAHGVTHVFGIVGSAFMDALDLFPTAGIRF
                     IPTVHEQGAGHMADGYSRASGRHGVCIAQNGPGVTNFVTSTAAAYWAHSPVVVITPET
                     GSNTMGLGGFQETEQLP"
BASE COUNT          416 a          733 c          718 g          474 t
ORIGIN      
        1 ctgcctttcc agcgctgcga tcgccgcgtc gaacacggaa tcaacgatga tcacggcatt
       61 ctcggacgag cagctcgtcg cgtaatcaaa ggtcttggac agcatgatct tgtacgcggc
      121 atcgtcgaga tccgccgacg cgtcgatgat caccggcgca tttccaagac cgacgccgat
      181 agctggcttt cctgatttgt aagcctggcg cacattcgcc tggctgcctg tgcagaccac
      241 gagatcggac agctccatca gccgcgtggt tgcctcccgc gtgaccggcg acggcagaac
      301 ctgcacgagg tcctctggta agccgatctt ctttagctct gcccgcataa ggtccactgt
      361 gggcttggtt gcaccccacg ccgtcggcgg cggcgcgatg acaatggcgt tgccgccctt
      421 gagcgccatc atcgccttgt tgacgggcgt cgccgaaggg ttcgttgacg gcgtgatggc
      481 cgcgaccacc ccgaccggct ttgcgtattt gacgatgccc ttttcgggaa tctcctcgat
      541 gattccggtg gacttcacgc gcaggagatc gcgcagtgtg ccgaaagtct tgcgctggtt
      601 cttgatgact ttgctctcga cgttaccgag cccggtcacc tcgacggcga tttcggccat
      661 ccggcgcgca ttttccggct tgtaaagaga ccaggcgata gccgtcacgg cctcgtcgat
      721 tcgcgcttga cctccggcag cgatttcgcg ggcataaaca gccattgcgc tgcgcgcccg
      781 ctggacgatt tccggaacga gatcaactgg cgcggcgggt gcattggatg ggcttagtgt
      841 gggcgtttcc tgcattcatg ccctccaaaa tttgagatgc cttgtctcat tttgtgtctt
      901 cggcgcccag cggtcaactc ctagtagatg tgtccaccgc ctcggttggc cgcgggatcc
      961 tgcccgcggc agagcgtagt tatcgtgcta tacgaagcga aatgagctcc acacacagga
     1021 atcgatcgga catgcgtggc ccaagcaacg gtcaggagcg aggctcggca ctcgacaaag
     1081 cgctagcggt cctggaagcg atcaccgcgc agtcacagcc aatcgggctt ccggacctca
     1141 gtgctcggct caaattgcca aggcaaacag ttcatcgcgt tctgacccac ctggagaagc
     1201 tggggcttgt cgttcgcgac ccatctcgcg accggttctc ggtgggcccc cgcctgtcga
     1261 cgctcgctct caacgcgctg cgcacacaca atcagtctgc acccattcac gccatcttgc
     1321 agcagctggt cgacgagatc gaggagacct gcaacgtcgg tgtgctggac ggtttacaat
     1381 tcgtgtatct cgagcgcatc gagtgtcagt ggccactgcg cgttcatctg caagcgggca
     1441 gccgcgtgcc ggcacattgc acctccggcg gcaaggtgat gctggcccac ctcgacgaag
     1501 ccgttcgagc gcggctgctc cgcaatgtca cgctgaaacc ctgcacgaag tatacgatca
     1561 ctcggacgga agagcttgag gccgagctgg cggaggtgcg ccggcgcggc tacgcggtga
     1621 acaatcagga gttcatgatc ggtatcatcg gcgttgcggt accgatcgcg gattgccagg
     1681 gtcgcgtgct ggcggcgctc gccgtccacg gaccttcgcc gcgcctcgac ctcgaccggg
     1741 cgatcggctt tgttcctcgg ctcaaggagg cttcagccat catggcggat gcctggacca
     1801 gcggtcccga agttgctgat acaccaacgt cttcacgggc tagcagtgcg aggacagctc
     1861 cggcgcgcgt cggcgattga cagctttcgt ggccgcgtgc cacaaattga gacgtggcgt
     1921 cttgattttt gagacaccaa acggctgcga gttgcagcgg cagcattcca gggagaccgc
     1981 ccatgacgag aatgaccgcg agtgaagcgt ttgtcgaaac tctggttgcc cacggggtga
     2041 cccacgtttt cggcatcgtc ggctcagcgt tcatggatgc gctggatctg tttccgacgg
     2101 ccggcattcg cttcattccg acggtgcacg agcagggcgc ggggcacatg gccgatggct
     2161 acagccgcgc ctcgggccgc cacggcgttt gcatcgcgca gaacggcccc ggcgttacga
     2221 atttcgtgac ctcaaccgcc gcagcttact gggcgcactc gccggtcgtc gtgatcactc
     2281 cggaaacggg ctccaacacc atgggcctcg gcggctttca ggagacggag cagctgccga
     2341 t
//
LOCUS       DASUMG010000366         6879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_609754, whole genome shotgun sequence.
ACCESSION   DASUMG010000366 DASUMG010000000
VERSION     DASUMG010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6879
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609754"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4297
                     /gene="gltB"
                     /locus_tag="VFX46_06030"
     CDS             <1..4297
                     /gene="gltB"
                     /locus_tag="VFX46_06030"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HEX5600173.1"
                     /translation="DNSCLSDTVRATEPVHRQIFIARPKAIKDQDTFERRLYIARKVV
                     SNALYYAYKDRDIGHYTVSLSSRTLVYKGMFLSYQLKAYYKDLSDKRLSSALALVHQR
                     FSTNTFPSWKLAHPFRMVAHNGEINTLRGNLNWMAARQASVSSPLFGDDIHKLWPISY
                     EGQSDTACFDNALEFLVMGGYSLAHAAVMLIPEAWAGNPLMDEKRRAFYEYHAALMEP
                     WDGPAAVAFTDGRQIGATLDRNGLRPARYLVTKDDRVIMSSEAGVLPIPEDQIVSKWR
                     LQPGKMLLIDLEQGRIISDDELKAELAARHPYEQWLRRTQIKVSELPLPKKPRRRKSN
                     VDLLDLQQAFGYTQESLKLLMAPMVQTGQEAVGSMGTDTPVSALSNKPKLLHTYFKQN
                     FAQVTNPPIDPIREELVMSLVSFIGPRPNLLDLEGTSKLKRLEVSQPILTNDELERIR
                     QIGDVPDNHFTSITIDITYPVEQGAAGMGPALDVICAEAEEAVRSGDYNIIILSDRAT
                     GPDRIPIPSLLATSAVHHHLIRQGLRTSVGLVVETGEAHEVHQFATLAGYGAEAINPY
                     LAFETIEALLPQLDEKLTLKEAIKRYIKAIDKGLLKVMSKMGISTYQSYCGAQIFDAV
                     GLKSSFVKKFFTGTNTTVEGVGLRHIARETVERHRQAFGDSPVLKHSLDVGGEYAYRV
                     RGEAHMWRPTVIADLQHAVRGNLPEKYRAFAQQVNEQSEQLMTLRGLFRIKSAEEIGR
                     KPIPLEEVEPAANIVRRFSTGAMSFGSISREAHTTLAIAMNRIGGKSNTGEGGEESDR
                     YKPLPNGDSMRSAIKQVASGRFGVTTEYLVNADVIQIKMAQGAKPGEGGQLPGHKVDA
                     IIAKVRHSTPGVGLISPPPHHDIYSIEDLAQLIFDLKNVNPNADISVKLVSEVGVGTV
                     AAGVAKARADHVTISGFEGGTGASPLTSIKHAGSPWEIGLAETHQTLVANRLRGRIAV
                     QVDGGVRTGRDVAIGAMLGADEFGFSTAPLIAMGCIMMRKCHLNTCPVGIATQDPVLR
                     AKFRGKPEHVINYFFFVAEEVREYMAALGFRTFDEMIGQSDVLDKDRAIRHWKARGLD
                     FSKLFYKPEVDSSVAIYNCERQDHGLENVLDRKLIELAKPALEERKPVRHELKITNRD
                     RAVGAMLSGEIAKRYGHEGLREDTIWLTFEGTAGQSFGAWLAHGVTLDLVGEANDYVG
                     KGLSGGKIIVRPHPNSGIKPEESIIVGNTVLYGAISGECYFRGIAGERFAVRNSGAVA
                     VVEGTGDHGCEYMTGGVVVVLGPTGRNFAAGMSGGIAYVLDEDGKFEQRCNLAMIDLE
                     PIEAEEEVMRRLANQGGDLESHGLVDILSDMDRKDAERLHQLIERHARYTNSAKARMI
                     LADWRNWLPKFKKVMPVEYREALRKIAEHQAYDKTGLEVLEIGLPRTHKPAAE"
     gene            4350..5786
                     /locus_tag="VFX46_06035"
     CDS             4350..5786
                     /locus_tag="VFX46_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HEX5600174.1"
                     /translation="MGKVTGFLEFERVDRGYEPVEARVKHWREFVVPLPENEVRTQAA
                     RCMDCGIPYCHNGCPVNNQIPDWNDLVYRGDWRTAALNLHSTNNFPEITGRVCPAPCE
                     ASCTLNIDDKPVTIKTIECSIADRAFEEGWIIPQPPERKTGKRVAIVGSGPAGLAAAQ
                     QLARVGHEVHVYEKNAKPGGLLRYGIPDFKLEKHIVDRRIKQMEAEGVVFHCGVRVGV
                     DVSAKQLEDEFDAVLLTGGSEQPFDFFANAPGRDLDGIHFAMEFLPQQNRRVSGESLG
                     GVKEITAKDKHVVVIGGGDTGSDCIGTSIRQGAKSVTQLEIMPKPPEKENKELTWPHW
                     PVKLRISTSQAEGARTEYSISTTGFSGRNGKVEKLHYVRVDAKLQPIPGTEGEMPADL
                     VLLAMGFAGPVENDLIKELGLNVVPRGRFKGLDADDRDYRIKGSKRLFAAGDMRRGQS
                     LVVWAIREGRQAAHAIDKALMGHTDLPR"
     gene            complement(5833..>6879)
                     /locus_tag="VFX46_06040"
     CDS             complement(5833..>6879)
                     /locus_tag="VFX46_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600175.1"
                     /translation="DRVALRAPDGRRVNVGTEEWKEEYGRRIDRITRALKRRGAAVYW
                     VGLPIVRRPDVNEDVRMLNSVMRERALLNNVKFIDVYEAFADETGQYTQYGPDLTGKQ
                     RLLRHGDGISLTTVGNKKLAHYVEREIKRDLIQAKNERDIPLAGSEAEQRRISPKAQQ
                     SEQAGGAAREAQKGTTPPSAKAPNFGFLPGNPRVGELKAENSTVSIKVPGNGGRSEEI
                     TVEIVRPAIPASVVALVSRRQSADRPAQLGDTVPDTLPGGLTVLSSITPGLAAGSQAH
                     RRRLAPTQSPFFKVLVKGERIPPKPGRADDFTWPPPDPVGPRLEQRSEANSGPKIPLP
                     TRNPEAADRSPSHG"
BASE COUNT         1311 a         2182 c         2171 g         1215 t
ORIGIN      
        1 cgacaactcc tgcctgtccg ataccgtgcg ggcaactgag cctgttcatc ggcagatttt
       61 cattgcccgt cctaaggcga taaaggatca ggacaccttt gagcgtcggc tctacatcgc
      121 gcgcaaggtc gtctccaacg cgctctacta cgcctacaag gaccgtgaca tcggccacta
      181 caccgtgtcc ctgtcgagcc gcacgctggt ctacaagggc atgttcctgt cctaccagct
      241 gaaggcctac tacaaggacc tctcggacaa gcggctttcc tccgcgctcg cgctggttca
      301 ccagcggttt tcaaccaaca ccttcccctc gtggaagttg gcccatccct tccggatggt
      361 cgcgcacaac ggcgagatca acacgttgcg cggcaacctc aactggatgg cggcacggca
      421 agcgtccgtc tcgtccccgt tgttcggcga cgacattcat aagctctggc cgatctccta
      481 cgaagggcaa tcggacactg cctgcttcga taacgccctc gaattcctgg tgatgggggg
      541 ctacagcctt gcccacgcgg cggtgatgct catcccggag gcctgggccg gcaatccgct
      601 catggatgaa aagcgccgcg ctttctatga gtatcatgcc gcgctgatgg agccgtggga
      661 cggcccggct gccgtcgcct tcaccgacgg tcggcagatt ggcgccacgc tcgaccgtaa
      721 tggcttgaga cccgcacgct acctggtgac caaggacgac cgggtgatca tgtcgtccga
      781 ggccggcgtg ctgccgattc ccgaggatca gattgtctcc aagtggcggc tgcagccagg
      841 caaaatgctg ctcatcgatc tcgagcaggg ccgcattatc tccgacgatg aactgaaggc
      901 cgagctcgca gctcgccatc cttacgagca gtggctgcgc cgcacgcaga tcaaggtcag
      961 cgaactgccg ctacccaaga agccaaggcg gcggaagtcc aacgtcgatc ttctcgatct
     1021 gcagcaggcc ttcggctaca cgcaggaaag cctcaagctc ctgatggcgc ctatggtcca
     1081 gaccggccag gaagccgtgg gctccatggg cactgacacg ccggtctcgg cgctgtccaa
     1141 taaaccgaag ctgctgcaca cctacttcaa gcagaacttc gcacaggtga cgaacccgcc
     1201 gatcgatccg atccgcgagg agctcgtcat gagccttgtg tcgttcatcg gcccacggcc
     1261 gaacctgctc gacctggagg gcacctccaa gctgaagcgg ctcgaggtca gccagccgat
     1321 tctcacgaat gacgagctgg agcgcattcg tcagatcggt gatgtgccgg acaatcactt
     1381 cacgtccatt acgatcgaca ttacctatcc ggtggagcaa ggcgctgccg gcatgggtcc
     1441 cgcccttgac gtgatctgcg ccgaggccga ggaggccgtc cgctcgggcg attacaacat
     1501 catcatcctg tcggaccgcg cgaccgggcc ggatcgcatt ccgattccgt cgttgctcgc
     1561 gacctctgct gtgcaccatc acctcatccg gcaagggctg cggacgtctg ttggcctcgt
     1621 ggtcgagacc ggcgaagcgc acgaggtgca ccagttcgcg acgctggcgg gctacggtgc
     1681 tgaggcgatc aatccctacc tcgccttcga gacgatcgaa gctctgctgc ctcagctcga
     1741 cgaaaagctg acgctgaagg aggcgatcaa gcgctacatc aaggccatcg acaagggcct
     1801 gctcaaggtc atgtcgaaga tgggcatctc gacctatcag tcgtactgcg gtgcccagat
     1861 cttcgacgcg gtcggcctca agtcgagctt cgtgaagaag ttcttcaccg gcaccaacac
     1921 gacggtcgaa ggcgtcggcc tgcgccatat cgcccgcgag acggtcgagc gacaccgcca
     1981 agcctttggt gactcgccgg ttctgaagca ttcgctcgac gttggcggtg aatatgccta
     2041 tcgcgtgcgc ggcgaagcgc acatgtggcg gccgaccgtc atcgccgacc tgcagcacgc
     2101 ggtgcgcggc aacctgccgg agaagtaccg ggccttcgcg cagcaagtta atgagcagtc
     2161 tgaacagctc atgacgctgc gcgggctgtt ccggatcaag tctgcggagg agatcggtcg
     2221 caagccgatc ccgctggagg aggttgagcc agcggccaat atcgtgaggc gcttctcgac
     2281 cggcgcgatg agcttcggct ccatcagccg cgaggcgcat accacgctgg ccatcgccat
     2341 gaaccgcatc ggcggcaagt cgaacacggg cgaaggcggt gaagagtccg atcgttacaa
     2401 gccgctgcca aacggcgact ccatgcgctc ggccatcaaa caggtggcct caggccgatt
     2461 tggggtgacg acggaatatc tcgtcaacgc cgacgtcatt cagatcaaaa tggcccaagg
     2521 cgccaagccc ggcgagggcg gccagctgcc cggtcacaag gtggatgcga tcatcgccaa
     2581 ggtgcgccac tcgacgccgg gcgtcggcct catctcgccg ccgccgcacc acgacatcta
     2641 ctccattgag gatctggcgc agctcatctt cgatctgaag aacgtcaatc cgaacgcgga
     2701 catctcggtg aagcttgtct ccgaggtggg cgtcggcacc gtcgcggcgg gcgtggcgaa
     2761 ggcgcgcgcc gatcatgtca ccatctcggg ctttgaggga ggcacgggcg cgtcgccgct
     2821 cacctccatc aagcacgcgg gttctccgtg ggagatcggc ttggccgaga ctcaccagac
     2881 tcttgtggcg aaccgcctgc gcggccgcat cgcggtccag gtggacggcg gcgtgcgcac
     2941 ggggcgcgac gtggcgatcg gtgccatgct gggcgccgac gagttcggct tctccaccgc
     3001 gccgctgatc gcgatgggct gcatcatgat gcgcaagtgc catctcaaca cttgccccgt
     3061 cggcatcgcg acgcaggacc cggtgctgcg cgcgaagttc aggggcaagc cagagcacgt
     3121 catcaactac ttcttcttcg tcgctgaaga agtgcgcgag tacatggcgg cgctcggctt
     3181 ccgcaccttc gacgagatga tcggccagtc cgacgtgctc gacaaggatc gcgccattcg
     3241 tcactggaag gcgcgcggcc tcgacttctc gaagctgttc tacaagccgg aggtcgatag
     3301 cagcgtcgcg atttacaact gcgagcgcca agaccacggc cttgagaacg tgctcgaccg
     3361 gaagctcatc gagctcgcca agccggcgct cgaagagcgg aagccggtgc gccacgagct
     3421 caagatcacc aaccgcgacc gtgccgtggg cgccatgctc tcaggcgaga tcgccaagcg
     3481 gtacgggcac gagggcctgc gggaggacac gatctggctc acgttcgaag gcacagccgg
     3541 acagagcttc ggcgcatggc ttgcacacgg cgtgaccctc gacctcgtcg gtgaagcgaa
     3601 cgactacgtc ggcaagggcc tgtcgggcgg caagatcatt gtccggccgc acccgaattc
     3661 cggcatcaag cccgaggagt cgatcatcgt cggcaatacg gtgctttatg gagccatcag
     3721 cggcgagtgc tacttccgcg gcattgcggg cgagcgcttt gctgtccgca actccggcgc
     3781 ggtggccgtc gtcgaaggta caggcgacca cggttgcgag tatatgaccg gcggtgtcgt
     3841 cgtcgtgctc gggccgacag gccgcaactt tgcggccggt atgtcgggcg gcatcgccta
     3901 cgtgctcgac gaggacggca agttcgagca gcgctgcaac ctcgccatga tcgacctcga
     3961 gccgatcgag gccgaggaag aggtgatgcg ccggctcgcc aaccagggcg gcgacctcga
     4021 aagccacggg ttggtcgaca tcctctccga catggaccgg aaggatgccg agcggttgca
     4081 tcagctcatc gagcgccatg cccggtacac gaactcggcc aaggcccgca tgatcctcgc
     4141 cgattggcga aactggctgc cgaaattcaa gaaggtgatg ccggtcgagt accgcgaggc
     4201 cctgcgcaag attgccgagc atcaggccta tgacaaaacg gggctggaag tgctggagat
     4261 cggcttgccc cgaacgcaca agccggcggc ggaataggtg cgctcgctaa aggaaagcct
     4321 cggacgcaaa ttgatttcag gaaactcaga tggggaaggt caccggcttc ttggagttcg
     4381 agcgcgtcga ccgcgggtat gagcccgtcg aggcgcgcgt gaagcactgg cgcgagttcg
     4441 tggtgccgct gccggagaac gaggtgcgca cgcaagcagc ccggtgcatg gattgcggca
     4501 tcccctactg ccacaacggc tgtccggtga acaatcagat cccggactgg aacgatctcg
     4561 tctaccgggg cgactggaga acggctgcgc tcaatctgca ctcgaccaat aatttcccgg
     4621 agatcacagg ccgcgtctgc ccagcgccgt gcgaggcgtc ctgcacgctc aacatcgatg
     4681 acaagccggt cactatcaag acgatcgagt gctccatcgc cgaccgcgcc ttcgaggaag
     4741 gctggatcat tccccagccg cccgagcgca agacgggcaa gcgcgtagcc atcgtcggtt
     4801 cgggaccggc tggcctcgca gccgcgcagc agctcgcgcg ggtaggtcac gaggtgcacg
     4861 tctatgagaa gaatgccaag ccgggcggcc tgctgaggta cggtattccc gacttcaagc
     4921 tcgagaagca cattgtcgat cggcgcatca agcaaatgga ggccgaggga gtcgtcttcc
     4981 actgcggcgt gcgcgtgggg gtcgatgtct ctgccaagca gcttgaggat gagttcgatg
     5041 ccgtgctgct taccggcggc tcggagcaac ctttcgactt cttcgccaat gcgcccgggc
     5101 gcgatctcga tggcatccat ttcgccatgg agttcctgcc gcagcaaaac cggcgggtgt
     5161 cgggtgaaag cctcggcggc gtgaaggaga tcacggccaa ggacaagcac gtggtcgtta
     5221 tcggtggcgg cgacaccggc tccgactgca tcggcacgtc catccggcag ggagcgaagt
     5281 ccgtcaccca gctggagatc atgcccaagc cgccggagaa ggaaaacaag gagctcacgt
     5341 ggccacactg gccggtcaag ctccgcattt cgaccagtca ggcggaaggt gcgaggacgg
     5401 agtattcaat ttcgaccacc ggcttttctg gcaggaacgg caaggtcgaa aagctgcact
     5461 acgtgcgcgt cgacgcaaag ctgcagccca ttcccggaac cgaaggcgag atgcctgcgg
     5521 acctggtgct gctcgccatg ggcttcgctg gcccagtcga gaacgacctg atcaaggagc
     5581 ttggcctcaa tgtcgtgccc cgtggccggt tcaaggggct cgatgctgac gatcgcgatt
     5641 accggatcaa gggcagcaag cggctctttg cggctgggga catgcggcgg ggccaatcgc
     5701 ttgtggtctg ggcgatccgc gaaggccggc aggcagcgca tgcgatcgac aaggcgctga
     5761 tgggtcacac cgatctgccg cgttaagaaa aactcaccgc aatagcgggg accggccata
     5821 gcagtgcccg cctcagccat ggctgggcga tcgatccgcg gcttccggat ttctcgtggg
     5881 cagaggaatt ttcggaccag agttggcttc cgagcgctgc tcgagccgcg gcccgaccgg
     5941 gtccggcggc ggccaggtga agtcatcggc ccggcctggc ttcggaggaa tgcgctcacc
     6001 ttttacgagc accttgaaga agggtgattg cgtcggcgcc agccggcggc ggtgagcttg
     6061 cgagccggca gccagccccg gcgtgatcga gctcaacact gtcaagccac ccggcaaagt
     6121 gtccggaact gtatcgccga gctgtgccgg gcggtctgcc gattgacgcc gggaaacgag
     6181 cgcaacgacc gaagccggaa tggcaggtct gacaatctca acggtgattt cctcgctgcg
     6241 gccgccgttg ccaggcacct tgatcgacac cgtggagttt tcagccttta gctctccgac
     6301 acgcggattg ccgggcaaaa aaccgaagtt aggagccttt gcacttggag gcgtggtccc
     6361 cttttgtgcc tcccgtgccg ctcccccggc ctgctcgctt tgctgggcct ttggcgagat
     6421 ccggcgctgc tccgcctcgc tgccagccag ggggatgtcg cgttcgttct tcgcctgaat
     6481 gagatcgcgc ttgatctcgc gctcgacgta atgcgccagc ttcttattcc ccacagtggt
     6541 caggctgatg ccgtcgccgt ggcgcagcag ccgctgcttg cccgtaagat cgggaccata
     6601 ctgagtgtat tgccctgtct cgtcggcgaa tgcttcgtag acgtcgatga acttcacgtt
     6661 gttgagaagg gcccgctccc gcatgaccga gttcagcata cgcacgtcct cgttcacgtc
     6721 gggacggcga acgatcggca ggccgaccca atagaccgct gcaccgcgcc gcttgagagc
     6781 ccgggtgatg cggtcgattc tccgtccgta ttcctccttc cactcctcgg tgccgacgtt
     6841 gacacgcctg ccatccggcg cgcgcagtgc gacccgatc
//
LOCUS       DASUMG010000367         2522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_209094, whole genome shotgun sequence.
ACCESSION   DASUMG010000367 DASUMG010000000
VERSION     DASUMG010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209094"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VFX46_06045"
     CDS             complement(<1..442)
                     /locus_tag="VFX46_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5600176.1"
                     /translation="MADTFDFVVVGGGSGGCTVAGRLSEDPNTSVAVLDAGGRNDNWV
                     VTTPFALVLMVAGNVNNWAFNTVPQKGLNGRIGYQPRGKGLGGSSAINAMVYIRGHRA
                     DYDRWASLGNAGWSFDDVLPYFKRSEDNADFGGEYHGKGGPLAVN"
     gene            770..846
                     /locus_tag="VFX46_06050"
     tRNA            770..846
                     /locus_tag="VFX46_06050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:804..806,aa:Met,seq:cat)
     gene            complement(803..2497)
                     /locus_tag="VFX46_06055"
     CDS             complement(803..2497)
                     /locus_tag="VFX46_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX5600177.1"
                     /translation="MRVALYARYSSDNQRDASIEDQFRICKERAARENWTIVASYKDA
                     GISGASMILRPGIQALLEDAQSRAFDMVLAEALDRISRDQADVATLFKQLRFAGVPIV
                     TLAEGEITELHVGLKGTMNALFLKDLAAKTHRGLRGRVEGGKSGGGLCYGYRVIKKLD
                     ERGDRIRGDREIDPAQADVIRRIFRDFASGISPRAIAMRLNAEGVAGPETKLWNDTTI
                     RGHLKRGTGIINNELYLGRLVWNRLHYVKDPSTGKRISRLNPESEWIVTEVPALRIVD
                     DELWQAAKAKQKEIAEKYVNVTAAIREHHSNNRINSARRPKSLFSGLVFCGCCDGPYT
                     LRGADRFVCSAHVSNGSCSNGRTLARSALERRVLDGLKDRLLAPDVVAQAMRAYAEEM
                     NRLNRERRASGDAWRAELAKVEKQIQGIVEAVKEGLFQRSMIAAMDALEARKAELTAQ
                     LADAPADTPDMLPSAAQLYAKKVAHLTEALNRPEDRAEAAQALRGLIERIVLRPGAKR
                     GQIDATLYGELGTVLSWTAARNADKGAQTKTPAASATGVSVSMVAGIGFEPMTFRL"
BASE COUNT          416 a          830 c          768 g          508 t
ORIGIN      
        1 tgttgaccgc cagaggcccg cccttgccgt ggtactcgcc gccgaaatcg gcgttgtcct
       61 cggaacgctt gaaatagggc agcacgtcgt cgaacgacca gccggcattg ccgagcgagg
      121 cccatcggtc gtaatcggcg cggtggccgc ggatatagac catcgcgttg atggcggacg
      181 agccgccgag ccccttgccg cgcggctgat agccgatgcg gccgttgagg cccttctgcg
      241 gcaccgtgtt gaaggcccag ttgttgacgt tgccggcgac catcagcacc agcgcgaatg
      301 gcgtggtgac cacccaattg tcgttccggc cgccggcgtc cagcaccgcc accgacgtgt
      361 tcgggtcttc cgacagccgt ccggccaccg tgcagccgcc cgagccgccg cccaccacca
      421 cgaaatcgaa tgtatccgcc acgcgtatcc cccgggtttt tctgttctgg cggcgccttt
      481 ggggccgcgc tttggggcca aaactcggct tttgcggcgt ttatggcaag ggcggttcca
      541 ggcagcgttt tcgccgtctc aaacccccgt ttttgctgtc ataaaatcct caaaagcgga
      601 gcctttcccc tttccaaagc aggtcgacgt tgatacatac ccctcgcgcc cgacggcttc
      661 ggcccgggtc gccttctcag gaagtctccg aacgggatcg atcggcgcca tctggcgttc
      721 gccgggatcg tttcggcacc ggcttttcga aggcagcgca acggtttaac gcggggtgga
      781 gcagcccggt agctcgtcag gctcataacc tgaaggtcat aggttcaaat cctatccccg
      841 caaccattga taccgacact cccgtagccg aggctgcggg agttttcgtt tgagctccct
      901 tgtccgcatt gcgagccgcc gtccagctca ggacagtgcc caactcccca taaagcgtgg
      961 catcgatctg cccgcgcttg gcgcccgggc ggagcacgat gcgctcgatc aggccacgca
     1021 gggcctgcgc ggcctcggcc cgatcttccg gacggttcag ggcctcggtc aggtgcgcga
     1081 ccttcttggc ataaagctgc gccgcgctcg gcagcatgtc cggggtgtcg gcgggggcat
     1141 cggcgagctg ggccgtgagc tctgccttgc gtgcttctag cgcgtccatc gctgcgatca
     1201 ttgagcgctg gaatagcccc tccttgaccg cttcgacgat cccctggatc tgcttttcga
     1261 ccttcgccag ctccgcgcgc caagcatcgc cgctcgcccg ccgttctcgg ttcagccggt
     1321 tcatctcctc ggcataggcg cgcatggctt gggcaaccac gtcgggcgcc agcagccgat
     1381 ccttcagccc gtccaggaca cggcgctcga gcgcgctccg cgccaaggtg cgtccgttcg
     1441 agcaggaacc gttgctcaca tgagccgagc agacgaaacg atccgcgccc cgcagcgtgt
     1501 atgggccgtc gcaacagccg cagaagacga gcccggagaa cagcgacttg ggacgccgag
     1561 cgctgttgat gcgattgttc gaatggtgct cgcggattgc agcggtcaca ttaacgtact
     1621 tctccgcaat ctccttctgc ttcgcctttg ccgcctgcca cagctcgtca tcgacgatac
     1681 ggagcgccgg cacctcggtg acgatccatt cggactccgg attgagccgt gagatgcgtt
     1741 tgccggtcga gggatccttg acgtagtgca gcctgttcca gacgaggcgg ccgaggtaaa
     1801 gctcgttgtt gatgatcccg gtgccgcgct tgagatgacc gcggatggtc gtgtcgttcc
     1861 acagcttggt ctccggtccg gccacgcctt cggcattgag cctcatggcg attgcccgcg
     1921 gactgatgcc agaggcaaag tcgcggaaga tgcgccggat gacatctgct tgggcgggat
     1981 cgatctcccg gtcgcctcgg atacgatcgc cccgttcgtc taatttcttt atgacgcggt
     2041 agccgtaaca cagcccgcca ccggactttc cgccctccac ccgccctcgc aagccgcggt
     2101 gcgtcttggc cgccagatct ttcaggaaaa gggcattcat cgtccctttt agcccgacgt
     2161 gcagctcggt gatctctccc tcggcaagcg tgacgatcgg gacaccggcg aagcgcaatt
     2221 gcttgaacag cgtcgccaca tccgcctgat cgcggctgat gcggtcgagt gcttcggcga
     2281 gcaccatatc gaacgcgcgg ctctgcgcgt cctcgagcaa cgcttgaatg ccagggcgga
     2341 ggatcatgct ggcgccggag atgccggcgt ccttgtagga cgcgacgatc gtccagttct
     2401 ctcgcgcagc gcgctcttta cagatgcgga attggtcctc aatcgaagcg tcgcgttgat
     2461 tgtcagatga gtatcgagcg taaagagcaa cgcgcataat tggctttccc tggcaatgcg
     2521 tt
//
LOCUS       DASUMG010000368         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_610027, whole genome shotgun sequence.
ACCESSION   DASUMG010000368 DASUMG010000000
VERSION     DASUMG010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610027"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..98)
                     /locus_tag="VFX46_06060"
     CDS             complement(<1..98)
                     /locus_tag="VFX46_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX5600178.1"
                     /translation="MTVTVRFAPSPTGRLHIGNVRTAVLNWLFARR"
     gene            complement(187..1008)
                     /locus_tag="VFX46_06065"
     CDS             complement(187..1008)
                     /locus_tag="VFX46_06065"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX5600179.1"
                     /translation="MRDDGSHDHTKAREAELTFEARALQLAEHIDIKGLEREGQLSTN
                     PLALSVGEKGMAVLFRFGAAVFIRMTPREQEEFIAGISSRLVEPTTVHESESVSITIR
                     PDAEDSVTLTGNVVLKDDAPERLLLVAEALAVSVALGYDEIRIAEAFDRIEPVANSLM
                     RGKLLRGSRAALVRQIGEALHIQQRLAGRIEAADRPDVLWDHPELERLWVKLADDYDL
                     AVRARAIGQKLEVIRETADTMADLLSTRTSHRLEWYIIILILVEVLLGLYDRFLK"
     gene            complement(1027..>2678)
                     /locus_tag="VFX46_06070"
     CDS             complement(1027..>2678)
                     /locus_tag="VFX46_06070"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEX5600180.1"
                     /translation="AEAAQLGADVIVFPELFITGYPPEDLVLKPAFAAAARRGLEDLA
                     RVVANGGPALIVGTVWAEEGRAYNSVALLDEGAIQAVRYKVDLPNYGVFDEKRVFHSG
                     PLPGPMNVRGVRIGVPICEDIWQEDVIECLQESGAEIIVSPNGSPFDRLKPDLRLNVA
                     VARITESGLPLAYLNQVGGQDELVFDGASFVLNSDASLAVQLPAWEEAVVVTEWEQQD
                     GTWWCKPGPKAVVEEDAAAAYQACMLGLRDYVDKNGFPGVVLGLSGGIDSALVAALCV
                     DALGPERVSCYMLPYRYTSNESLSDAAECAAALGVRYEVLPIEAPVAGFMSALERTFA
                     GREPDTTEENIQSRVRGTMLMAISNASGSMLVTTGNKSEMSVGYATLYGDMNGGFNPI
                     KDLYKLQVYRLARWRNAHVPKGGKGPAGIVIPENILTRVPSAELRPNQTDQDSLPPYE
                     VLDDILECLVEHEMPLPEIVARGHDPATVKRVERMLYLAEYKRRQSAPGVKITIRSFG
                     RDRRYPITNKFRESLEAPAGTQSAISAPRIVRPRADGGEVS"
BASE COUNT          489 a          860 c          840 g          489 t
ORIGIN      
        1 tgccggcgcg caaaaagcca gttcagcacg gcggtgcgca cgttgccgat atgaaggcgg
       61 ccagtagggc tcggggcgaa acggacggtt acggtcattc ggattccggt gctcgctgct
      121 cgcgtgacag gcaccctaca ccgcgcgtgc ccgtaggcaa acgcggccat atgggtcgcg
      181 aatcgctcat ttcaggaacc ggtcatacag gccgaggagc acctcgacca ggatcagaat
      241 gatgatgtac cactcgagcc tatggctcgt gcgggtggaa agcaggtcgg ccatggtgtc
      301 ggccgtctca cggatcacct cgagcttctg gccaatggcg cgcgcccgca ctgccaggtc
      361 gtagtcgtcc gcaagcttga cccagagacg ctcgagctcg ggatggtccc agagcacgtc
      421 tggtcgatcg gcggcctcga tgcggcccgc tagtcgctgc tgaatgtgaa gcgcctctcc
      481 gatctggcgg accagcgccg cacgactgcc tcggagcagc ttgccgcgca tgaggctgtt
      541 ggcgacaggc tcgatgcggt cgaatgcctc cgcaatgcgg atttcgtcgt aacccagggc
      601 cacgctgacg gccaacgctt ccgcgacgag cagcaagcgc tcaggcgcat cgtccttcag
      661 aacgacattc ccggtgagcg tgacggaatc ctcagcgtct ggccggatag tgatgctgac
      721 gctttcgctt tcgtgtaccg tcgtcggttc gaccaggcgg ctgctgatcc cggcaatgaa
      781 ctcttcctgc tcgcgagggg tcatgcggat gaacacggcc gcgccaaagc gaaacagcac
      841 cgccatgccc ttttcgccga ccgacaaggc gagcggattg gtggagagct gcccctcgcg
      901 ctccagcccc ttgatgtcga tatgctccgc aagctgcagc gctcgcgctt cgaacgtcag
      961 ctcggcttcc cttgccttcg tgtgatcgtg ggagccatca tcgcgcatgg ttgatggaag
     1021 atcggctcaa gacacctcgc cgccgtcagc gcgcggcctg acaatgcgtg gcgccgagat
     1081 tgctgattgt gtaccggccg gcgcctcaag gctttcacgg aacttatttg tgatcggata
     1141 acgccgatcg cgcccaaagc tgcggatcgt aatcttcacg ccgggagccg actggcggcg
     1201 cttgtactcg gcgaggtaaa gcatccgttc tacgcgcttc acggttgctg ggtcgtgacc
     1261 acgagcaacg atctcgggca atggcatctc gtgctcgacc agacattcca ggatgtcatc
     1321 gagcacctcg tatggcggca acgaatcctg gtccgtctgg ttcggccgta gctccgccga
     1381 gggaacgcgt gtcaagatgt tctccggaat gacgatgcca gctggcccct tgccaccctt
     1441 gggaacgtgc gcgttgcgcc aacgcgcgag gcgatagact tgcagcttgt agagatcctt
     1501 gatcgggttg aagccaccgt tcatgtcgcc atatagcgtg gcgtagccga ctgacatctc
     1561 ggacttgttg ccggtcgtca ccagcatgct gccggatgca ttcgagatgg ccatcagcat
     1621 cgtaccgcgg acgcggcttt ggatgttctc ctcggtagtg tccggctcgc gtccggcaaa
     1681 ggtccgctcc aatgccgaca tgaagcccgc gacgggtgcc tcgatcggta agacctcgta
     1741 tcgcacgccc agagctgcag cgcattcggc cgcatcggaa aggctttcgt tcgaggtgta
     1801 acggtagggc agcatgtagc agctcacgcg ctccggcccg agcgcatcca cacacaaggc
     1861 agccactagc gcagagtcga tgccgcctga caggccaagc accacgccag ggaaaccgtt
     1921 cttgtccacg taatcgcgca gcccgagcat gcaggcctga taggcagcgg ccgcatcctc
     1981 ctcgacgacg gccttggggc caggcttgca ccaccacgtt ccgtcctgct gctcccattc
     2041 ggtaacgacg accgcttcct cccaggcggg gagctgcaca gcgaggctgg catcggagtt
     2101 gagaacgaag gaggcgccgt cgaacacgag ctcgtcctga ccgccgacct ggttcaggta
     2161 tgccagcggc agcccgcttt cggtgatccg cgcgacagcg acattgaggc gaaggtccgg
     2221 cttcaaccgg tcaaagggcg agccgttggg cgagacgatg atctccgcgc cggattcttg
     2281 caggcactcg atcacgtcct cttgccagat gtcctcgcag atcggcacgc cgattcgcac
     2341 gccgcgcacg ttcatcggtc caggcagcgg gccggaatga aacacgcgct tctcgtcgaa
     2401 gacgccgtaa ttgggcaggt ccaccttgta gcgcacggct tggatggcgc cctcgtccag
     2461 cagcgccaca gagttgtagg cgcgcccctc ctccgcccat acggtgccga caatcaatgc
     2521 tgggccgccg ttcgcaacaa cacgggcgag atcttccagc cccctgcggg ccgccgcggc
     2581 gaacgcaggt ttgagcacga ggtcctcggg cgggtagccg gtgatgaaca gctcggggaa
     2641 gacgatgaca tcggccccga gctgggccgc ttccgcgt
//
LOCUS       DASUMG010000369         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_610469, whole genome shotgun sequence.
ACCESSION   DASUMG010000369 DASUMG010000000
VERSION     DASUMG010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610469"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VFX46_06075"
     CDS             <1..800
                     /locus_tag="VFX46_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX5600181.1"
                     /translation="RALTALIRSKGMPNAVIAHAKDGKFDLHGLKEEAREWPGLEGMD
                     LVPMVTSGQRFSWDETPWLWDKGFGRQDKAEFNVVAIDYGIKRNILRLLAGEGCKITV
                     VPATTSAEDILALKPDGVFLSNGPGDPAETGKYAVPVIRKVIESGTPTFGICLGHQML
                     GLAVGAKTKKMHQGHHGANHPVKDETTGKVEITSMNHGFAVDENTLPKGATQTHISLF
                     DGSNCGIALDGKPVFSVQYHPEASPGPRDSHYLFDRFADLMRTKKRA"
     gene            complement(1080..2111)
                     /locus_tag="VFX46_06080"
     CDS             complement(1080..2111)
                     /locus_tag="VFX46_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX5600182.1"
                     /translation="MNEISMIGLDLAKNVFQVHGVDASGKVVVQRQLRRGAVEKFFAK
                     LSPCTVGMEACGSAHHWARLIGRYGHEVKLMPPAYVKPYVKRNKNDGRDAEAICEAAS
                     RPTMRFVAVKSVEQQAMLSVHTTRALLVKQRTMVANALRAQLSEFGIVAAQGHKGLHE
                     LMARLQAPSQQIPEMMRSALLVLAGQWRSLDANERELEKQIVKAARCDREACRLMEAP
                     SVGPIIASMVLAKVTDARVFRSSRDFAAWVGLTGRDHGTGGKHRPGPISKQGDRVLRT
                     LLINGASAHLRQQMRRGVSDPWLRELLARRPYKVAMVAFAAKTARIIWAMLAKGEVYR
                     DRASASAAA"
BASE COUNT          417 a          770 c          669 g          410 t
ORIGIN      
        1 cccgcgccct gaccgcgctg atccgcagca agggcatgcc caacgccgtg atcgcccatg
       61 ccaaggacgg caaattcgac ctgcatggcc tcaaggaaga agcccgcgaa tggcccggcc
      121 tcgagggcat ggacctcgtg ccgatggtca cgagcggcca gcgcttcagc tgggacgaga
      181 cgccctggct gtgggacaag ggctttggcc gccaggacaa ggccgagttc aacgtcgtcg
      241 ccatcgacta cggcatcaag cgcaacatcc tgcgcctgct cgccggcgag ggctgcaaga
      301 tcacggtggt gccggcgacc acgtcggccg aagacatcct ggcgctgaag cctgacggcg
      361 tgttcctgtc gaacggtccc ggcgatcccg ccgaaaccgg caaatacgcg gtgcccgtga
      421 tccggaaggt gatcgagtcc ggtaccccga ccttcgggat ctgcctcggc caccagatgc
      481 ttggtctcgc cgtcggcgcc aagactaaaa agatgcatca aggccatcac ggcgccaacc
      541 atccggtcaa ggacgagacc accggcaagg tcgagatcac ctcgatgaac cacggttttg
      601 ccgtggacga gaacacgttg ccgaagggcg cgacgcagac tcacatctcg ctgttcgacg
      661 gctccaattg cggcatcgcg ctcgacggca agccggtgtt ctcggtgcag taccatcccg
      721 aggcctcgcc ggggccgcga gactcgcatt atctgttcga tcgcttcgcc gatctgatgc
      781 ggacgaagaa gcgggcttag ggccaggatc gtcatgtatg gacggccccc tctcggcaag
      841 ggcttttttg gtgtttcggc gaatgaatcg gggagcggtc atgtatacgg cctcgagatg
      901 cggccggtga ccgcgggccc tgatggagat cgcggatcga attccaatca ttgcggcgcg
      961 ctatgacgcg cgatgacgag acggagtgtt tcgatcccgg tttcctgacc atttgccatc
     1021 acaccttggt tgcccttccg ataggagtga gcgcgagcgc ggcggctgtc gcaccccaat
     1081 tacgcagcag ccgacgcgct cgcgcggtcg cggtaaactt ctcccttggc cagcatggcc
     1141 cagatgatcc gtgcggtctt ggcggcgaac gccaccatgg cgaccttgta gggccgccgt
     1201 gccagcagct cgcgcagcca gggatcgctg acgccgcgcc tcatctgctg ccgcaaatgg
     1261 gcgctggcgc cgttgatcag caacgtgcgc aaaacacgat ctccctgctt ggagatggga
     1321 ccgggtcgat gcttgccgcc ggtgccgtga tcgcggccgg tcagtcccac ccaggccgcg
     1381 aagtcacggc tggagcggaa cactcgggca tcggtgacct tcgccaatac catgctggcg
     1441 atgatcggac cgacgctggg cgcctccatc aaccggcagg cttctcgatc gcaacgtgcg
     1501 gccttcacga tctgcttctc caattcccgc tcgttggcgt cgagcgaccg ccattgcccg
     1561 gccagcacaa gcagggcgct gcgcatcatc tccgggatct gctggctcgg cgcctggagc
     1621 ctggccatca gttcatgcag ccccttgtgg ccctgtgccg ccacgatccc gaactcgctc
     1681 aactgtgccc gcaacgcgtt ggccaccatc gttcgctgct tcaccagcaa cgctcgcgtg
     1741 gtgtggaccg aaagcatcgc ctgctgctca acgctcttca ccgcgacaaa acgcattgtc
     1801 ggccggctcg ccgcctcgca aatcgcctcc gcgtcacgcc cgtcgttctt gttccgcttc
     1861 acgtacggct tcacataggc cggcggcatc agcttcactt cgtgcccata acggccgatc
     1921 aaacgagccc agtgatgcgc gctcccacac gcctccattc ccaccgtgca gggcgaaagc
     1981 tttgcaaaga acttctccac cgctccgcgt cgaagctgtc tctgcacaac caccttcccg
     2041 gacgcatcaa cgccgtggac ttgaaaaacg ttcttcgcca aatccagccc aatcatgcta
     2101 atctcgttca tggacggctc ccttgcctgg tcgaatcgac atcaccagtc tggcacgtag
     2161 atgccgtcgg gggccgtcca ccccatcatt gcgagcgaag cgaagcaatc catctaaccg
     2221 ctcgccgaga caatggattg cttcgtcgcg gagcctgtca tcgggc
//
LOCUS       DASUMG010000370         2177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_449562, whole genome shotgun sequence.
ACCESSION   DASUMG010000370 DASUMG010000000
VERSION     DASUMG010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449562"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..996
                     /locus_tag="VFX46_06085"
     CDS             22..996
                     /locus_tag="VFX46_06085"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX5600183.1"
                     /translation="MNDAGRKDYFDGLKIKLVAGNSNRSLTEEISSVLRVPLTKGVCR
                     RFADMEIFVEIQENVRGEDVYVIQSTSFPANDNLMELLILIDALKRASARRITAVIPY
                     YGYARQDRKPGPRTPISAKLVANLIERAGANRVMTLDLHAGQIQGFFDIPTDNLFAAP
                     VMVRDIKERFELSNLMVVSPDVGGVVRARALAKRIDAPIAICDKRRERPGESEVMNVI
                     GDVRGKRCILVDDIVDSGGTLCNAAEALLEKGALEVMAYITHGVLSGGAVARIQNSNL
                     RSLVITNSIEPTEAVKASKNIRVLSIAPLIAEAIARTSREESVSSLFY"
     gene            1166..1774
                     /locus_tag="VFX46_06090"
     CDS             1166..1774
                     /locus_tag="VFX46_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEX5600184.1"
                     /translation="MAEVVEIPATARPRAGKGAARAVRRQGKVPAVIYGEKKEPETIA
                     LDANELWKVVQRGRFLSTVLDINVDGQKRRVLPREIQLDPVKDLPIHVDFQRVGASDY
                     VRVVVPVRFINEGASPGLKRGGVLNVVRHEIEVTCPPDRIPEAITVDLTGLEIGRSIH
                     ISSVPLGEGVQPTIKRDFTVATIAGKKVEESAGEGEGEGEKK"
     gene            complement(1856..>2177)
                     /locus_tag="VFX46_06095"
     CDS             complement(1856..>2177)
                     /locus_tag="VFX46_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5600185.1"
                     /translation="DRIVESICPGARPLPLGHFGDGNVHYNVAQPIGMEKERFLELWD
                     KISSEVHALVVEMGGSISAEHGIGRMKRGDLERYKSPVEIDLMRRIKAAFDPNGILNP
                     GKVL"
BASE COUNT          422 a          683 c          662 g          410 t
ORIGIN      
        1 gggggcaaag tggtgtctgc aatgaatgat gctggccgga aagactactt cgacggactg
       61 aagattaagc ttgtcgccgg taacagcaat cggtcgctga ccgaggagat cagctcggtc
      121 ttgcgcgtgc ccttgaccaa gggcgtgtgc cgccgctttg ccgacatgga gatcttcgtc
      181 gagatccagg agaacgtccg tggcgaggac gtctatgtca tccaatcgac gtcttttccc
      241 gccaacgaca atcttatgga gttgctgatc ctcatcgatg cgctgaaacg ggcgtcggcc
      301 cggcgcatca cggcggtgat cccctactac ggatatgccc gccaggatcg taaacccggc
      361 ccgcgcacgc ccatttccgc aaagctcgtc gccaacctca tagagcgcgc cggtgcgaac
      421 cgggtcatga cactcgacct gcacgccggc cagatccagg gcttcttcga tatcccgacc
      481 gacaacttgt tcgccgcacc cgttatggtg cgcgacatca aggagcggtt cgagctcagc
      541 aacctcatgg tcgtttcgcc ggacgtgggc ggtgtcgtcc gcgcccgcgc actcgccaag
      601 cgcatcgatg ccccgattgc catctgcgac aagcggcgcg agcgccccgg cgagagcgag
      661 gtgatgaacg tgatcggcga tgtgaggggc aaacgctgca tcctggtcga tgacatcgtc
      721 gacagcggcg gcacgctctg caatgcggcc gaagccctcc tcgagaaggg cgcgctcgag
      781 gtcatggcct atatcactca cggggtgctc tcaggcggtg cggtcgcccg cattcagaac
      841 tcgaatttga gatccctcgt gatcacgaat tcgatcgagc ccaccgaggc ggtgaaagca
      901 tcgaagaaca tccgtgtgct ctccatcgcg ccgctcatcg ccgaggccat tgcccggact
      961 tcccgtgagg agagcgtgtc gagcctgttc tactgagcgg cgggcgacaa ctgagacgcg
     1021 cggaacctgc ctcatccgat tgtcaactgc cgtcctcgcg ggtataaggc ccgcatctca
     1081 gtgacatgag acgatccttc ggcacccttg gaggccgggg ataaacgcgg gccgttaccg
     1141 gccctttctt cattggagag cagccatggc tgaagttgtc gaaatcccgg ccacggcgcg
     1201 tcccagggct ggtaaggggg ccgcccgggc agtgcgtcgt caggggaagg taccggccgt
     1261 aatctacggc gaaaagaagg agcccgagac gatcgccctc gacgccaacg agctttggaa
     1321 ggtggtgcag aggggtcgct ttctgtcgac ggttctggac atcaacgtcg acggccagaa
     1381 gcgccgcgtt ctgccgcgcg aaatccagct tgatcccgtc aaggatctcc ccatccacgt
     1441 tgattttcag cgcgttggcg catccgacta cgttcgcgtc gtggttccgg ttcgcttcat
     1501 caacgagggt gcctcccctg gcctgaagcg tggtggcgtg ctcaacgtcg tccgccacga
     1561 gatcgaggtt acttgcccgc cggaccgcat tccggaggcc atcacggtcg acctcactgg
     1621 tcttgagatt ggccgcagca ttcacatctc gtccgtgcca ctgggcgagg gcgtgcagcc
     1681 gaccatcaaa cgtgacttca ccgtcgccac catcgccggc aagaaggtcg aagagagcgc
     1741 tggtgaaggt gagggtgaag gggaaaagaa atagcctgca gcattgggag catcgtaagg
     1801 tcgtgagtcg ggcagcaccc gagctcacga cctcacgaca tccgatcgct cccgatcaaa
     1861 gcaccttccc cggattgagt attccgttcg gatcaaacgc cgccttgatc cgccgcatca
     1921 agtctatctc caccgggctc ttgtagcgct ccagatcgcc gcgcttcatg cggccgatac
     1981 catgctctgc cgaaatggag ccgcccatct ccacgacaag tgcatgcacc tcgctgctga
     2041 tcttgtccca aagctcgagg aaccgctcct tttccatccc gatcggctgg gcgacgttgt
     2101 agtggacatt gccgtcgccg aagtgaccga gcggtagcgg gcgcgccccg ggacagatgg
     2161 actcaacgat gcggtcg
//
LOCUS       DASUMG010000371         2652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_48875, whole genome shotgun sequence.
ACCESSION   DASUMG010000371 DASUMG010000000
VERSION     DASUMG010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48875"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFX46_06100"
     CDS             <1..576
                     /locus_tag="VFX46_06100"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEX5600186.1"
                     /translation="EFRLRTVELVASYSGQAGAGLMQSADSNTGAPEVQLERVRAQRK
                     VVVTSKDDQSATGDWADFNLKTNTVVLGGDVTLTQGRNVVRGPRLVIDMTTGLSRMET
                     SKAGAQAAAPSTGDREAERGTNAPGEKGWPAGACGGRMCAVFYPKDFEAMAKRRKAEP
                     QGKAPPAPQPGPQGEAASSWSSTTIPAGTAE"
     gene            620..1480
                     /gene="lptB"
                     /locus_tag="VFX46_06105"
     CDS             620..1480
                     /gene="lptB"
                     /locus_tag="VFX46_06105"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX5600187.1"
                     /translation="MKLFPFRRRGVVSGTRPDASDANGPSLNGFAGPDDDADTGTEQP
                     YAAEGWLTVHNVEKSYRKRKVVRGVSLAVGRGEAVGLLGPNGAGKTTVFYMITGLVPA
                     DAGSIYIDGRDATHLPMYRRARLGIGYLPQEASIFRGLTVEQNIMSVLELVEPDRRKR
                     REKLDSLLEEFNIARLRKLPSIALSGGERRRCEIARALASGPSYMLLDEPFAGIDPIA
                     VGDIQELVRHLTQRGIGVLITDHNVRETLSLIDRAYIIYDGQVLTQGSPEEIIKNEDV
                     RRVYLGDMFG"
     gene            1592..>2652
                     /locus_tag="VFX46_06110"
     CDS             1592..>2652
                     /locus_tag="VFX46_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX5600188.1"
                     /translation="MTPQLQQAIRLLQYSNLELNAFVEAELERNPLLERVDDAPGSDP
                     REPLGTPDPVDDGEGPSSGAESAKAIDSLDAELDSVFPDATPTDLGSSYQESGWASLR
                     QSRTSGDDDIDFEAIAASDLTLKDHLRTQLPFAVTEPADRLIGQFLIEMVDEAGYLTG
                     DLETVARKLGTTIAHVERALSALQTLDPPGVFARNLAECLALQLKDKNRYDPYIARLL
                     DNLDLLAAHNLPALRKVVGVDMDDLLDMIAEIKRLNPKPGLKFGSAQVVPVIPDVIVK
                     AARDGSWRVELNSETLPRVLVDRTYYARVSRTSRDAQEKSYLLDCLQSANWLVKSLDQ
                     RARTILRVAEEIVRQQDAF"
BASE COUNT          538 a          806 c          842 g          466 t
ORIGIN      
        1 gagttcaggc ttcgtactgt cgagttggtg gcaagctata gcggtcaagc tggtgcaggc
       61 ctgatgcaat cggctgattc caacaccggc gctccggaag tgcagctcga acgtgtccgt
      121 gcacagcgga aggttgtcgt gacatccaaa gacgaccaaa gcgccacggg tgactgggcg
      181 gatttcaatc taaagacgaa tacggttgtg ctcggtggcg acgttaccct tacccaaggt
      241 cgtaacgtgg ttcgcggtcc acggctggtg atcgatatga ccaccggact gagccggatg
      301 gagacttcca aggctggagc gcaagcggcg gcaccatcca caggtgatcg cgaagccgaa
      361 cgcggcacga atgcccctgg cgaaaagggg tggcccgccg gggcttgtgg cggaaggatg
      421 tgtgccgtct tctatccgaa ggacttcgag gcgatggcca agcggcgtaa ggccgagccc
      481 caaggcaagg ctcccccggc tccccagcca ggcccgcaag gagaagcggc ttcaagctgg
      541 agctctacca cgataccggc gggtaccgcg gaatgagcca ggtcgacgat cagcataccg
      601 ctagcgggac ggtagaaccg tgaagctgtt tccctttcgt cgtcgagggg ttgtttcagg
      661 aaccaggccg gatgcgtccg atgcgaacgg gcccagcctc aatggttttg cgggcccgga
      721 tgacgatgca gacacaggaa ctgagcagcc ctatgctgca gagggctggc ttaccgtcca
      781 caacgtggaa aaatcgtatc gcaagcgcaa ggttgtgcgc ggcgtaagcc ttgccgtcgg
      841 ccgaggcgaa gcggtggggc tgctcggtcc caacggcgcc ggcaagacaa ccgtcttcta
      901 catgatcacc ggcctcgtcc cggccgatgc gggctccatc tacatcgatg gccgggacgc
      961 cacacacctg cccatgtatc gccgcgcgcg cctcgggatc ggctatctgc cacaggaagc
     1021 gtcgatcttc cgaggcctta cagtcgagca aaacatcatg tcggtgctgg agctggttga
     1081 gcccgaccgg cggaagcggc gtgaaaagct cgacagcctg ctggaggaat tcaatatcgc
     1141 ccgactgagg aaattacctt ccattgccct atcgggcggt gagcggaggc gatgtgagat
     1201 tgccagggcc ctggcgagcg gtccctccta catgctgctc gacgagccat tcgccggcat
     1261 cgatcccatt gcggtcggcg acatccaaga gctggttcgg cacttgaccc aacgcggcat
     1321 cggtgtgctc atcaccgacc ataatgtccg cgagacgctg agcctcattg accgcgccta
     1381 catcatctac gatggacagg tgctgaccca aggcagcccg gaagaaatca taaagaatga
     1441 ggacgttcgg cgagtttatc tcggcgatat gttcggttaa tcaggcttgg cgcatcatgt
     1501 ggcaggcgcc gcgcggagca tgggcggcca gggttatgcg tcaatggtac tgacggccag
     1561 gttagaagtt cggcagacgc agcagctggt gatgacaccc cagctgcaac aggccattcg
     1621 gctgctgcaa tattcgaacc tggagctcaa cgctttcgtc gaagccgaac tcgagcgcaa
     1681 tccgctgctg gagcgcgtgg acgacgcccc cgggagcgat ccgcgtgagc cgcttggcac
     1741 accggaccct gtggatgacg gcgagggacc ttcctcgggt gcagagtccg cgaaggccat
     1801 cgatagcctg gatgcggagc tcgacagcgt ttttcccgac gcgaccccaa ctgatctggg
     1861 ctcaagctac caggagagcg gatgggcttc actgcgtcag tcgaggacgt caggcgacga
     1921 cgatatcgat ttcgaggcga ttgcggcgtc ggaccttacc ctcaaggacc atctgcgcac
     1981 ccaattgcct ttcgccgtga cggaaccggc ggaccggctc atcggccagt tcctcatcga
     2041 gatggtggac gaggcgggct acctgacggg cgacctggag acggtggccc gcaagctggg
     2101 gacaacgatc gcccatgtcg agcgcgccct ctcggcctta cagacgctcg atccgcccgg
     2161 cgtgttcgct cgcaacctgg cagagtgcct cgcgctgcag ctgaaagaca agaaccgcta
     2221 cgatccctac atcgcccgcc tgctcgacaa tctcgatctg ctcgccgccc acaacctccc
     2281 cgccttgcgc aaggtcgttg gcgtcgacat ggacgacctg ctggacatga tcgcggagat
     2341 caagcggctc aacccgaagc cggggctgaa attcggatcc gcgcaggtgg tccctgtcat
     2401 ccccgacgtc atcgtgaagg cagcgagaga tggcagctgg cgcgtcgagc tgaactccga
     2461 gaccttgccg cgcgtcctgg tcgatcgcac ctactacgcg cgcgtctcga gaacgtcgag
     2521 agatgcgcag gagaagtctt atctgttgga ctgcctccaa tcagccaact ggctggtcaa
     2581 gagcctcgac caacgcgcac ggacgatcct gcgggtcgcg gaagagatcg tgcgccagca
     2641 ggacgcgttc tt
//
LOCUS       DASUMG010000372         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_851492, whole genome shotgun sequence.
ACCESSION   DASUMG010000372 DASUMG010000000
VERSION     DASUMG010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851492"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFX46_06115"
     CDS             <1..555
                     /locus_tag="VFX46_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5600189.1"
                     /translation="LVDEIEETCNVGVLDGLQFVYLERIECHWALRVHLQAGSRVPAH
                     CTSGGKVMLAHLDDAVRARLLRNVTLKPCTEHTITQTTELEAELAEIRRRGYAVNNQE
                     FTIGIIGVAVPIADRQGRVLAALAVHGPSPRLDLERAIGFVPRLKQAAAVMADAWTGG
                     PDVADTPLSSRATSVGTAPARVGD"
     gene            655..2409
                     /gene="xsc"
                     /locus_tag="VFX46_06120"
     CDS             655..2409
                     /gene="xsc"
                     /locus_tag="VFX46_06120"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HEX5600190.1"
                     /translation="MTRMTASEAFVETLVAHGVTDVFGIVGSAFMDALDLFPTAGIRF
                     IPTVHEQGAGHMADGYSRASGRHGVCIAQNGPGVTNFVTSTAAAYWAHSPVVVITPET
                     GSNTMGLGGFQETEQLPIFSKITKYQAHVSRADRMAELTARCFDMAMSERGPTQLNIP
                     RDMFYGEADYEIPRPKKIARGPGADESLDEAAELLASAKFPVMVAGGGVIFSGGIEEA
                     KALAEYLQMPVVNSYLHNDSFPADHPLWCGPLGYQGSKAAMKIIAEADVVLALGTRLG
                     PFGTLPQYGLDYWPRNAKIIQVDTDHRMLGLVKQISVGIVGDAKAAARALHHRLANRD
                     PVARRNASERLEKIKAEKDAWEKELSEWTHERDPWSLEVAKDSPYMHPRQMLRELEKA
                     LPKNAMVSTDIGNICSVSNSYLRFNQAPSMFAAMSFGNCGYALPTIIGAKVACPDRPA
                     IAYVGDGAWGMSFGEILTCVRENIPVTAVVFHNKQWGAEKKNQVDFYNRRFEGVDLKN
                     PSWAEVARSLGAEGVTVSNLEQVGEALEKAVDAQMKQGKTTVIEMMVTQELGDPFRRD
                     ALRKPRRLLEKYKHTNVE"
BASE COUNT          477 a          809 c          807 g          437 t
ORIGIN      
        1 ctcgtcgacg agatcgagga gacctgtaat gtcggagtgc tggatggcct acaatttgtg
       61 tatctcgaac gcatcgagtg tcactgggct ctccgcgtcc atctgcaggc gggcagccgc
      121 gtgccggcac attgcacctc cggtggcaaa gtgatgctgg cccatctcga tgacgcggtt
      181 cgagcacgac tgctccgcaa cgtcacgctg aagccctgca cggagcacac catcacgcag
      241 accactgaac ttgaggccga gctggcagag attcgccggc ggggctacgc ggtgaacaat
      301 caggagttca cgatcggcat catcggcgtt gcggtgccga tcgcggatcg ccagggtcgg
      361 gtgctggctg cgctcgccgt tcatggacct tccccgcgcc tcgacctcga gcgggccatc
      421 ggcttcgttc cgcgactcaa gcaggctgca gccgtcatgg ccgatgcgtg gacaggcggt
      481 cccgacgttg ccgacacacc cttgtcttca cgcgcgacta gcgtggggac cgccccggcg
      541 cgcgtcggtg attgacagct tttgtaaccg cgtgccacta attgagacga gacgtcttgt
      601 tttccgagac acagaaggct gcttgttagc gggagcatcc cagggagacc gcccatgacg
      661 agaatgaccg cgagtgaagc gttcgtcgaa actctggttg cccatggcgt caccgacgta
      721 tttggcatcg tcggctccgc gttcatggac gccctcgacc ttttcccgac agccggcatc
      781 cgcttcatcc cgacggtgca cgagcagggc gcggggcaca tggcggacgg ctacagccgc
      841 gcctcgggcc gccatggcgt ttgcatcgcg cagaacggcc ctggcgtgac gaatttcgtg
      901 acctcaactg ccgcagctta ctgggcgcac tcgccggtcg tcgtgatcac tccggaaacg
      961 ggctccaaca ccatgggcct cggcggcttt caggagacgg agcagctgcc gatcttctcc
     1021 aagatcacga agtatcaggc ccacgtctcc cgcgccgacc gtatggcgga gctcacggcc
     1081 cgctgctttg acatggccat gagcgagcgc gggcccacgc agctcaacat cccgcgcgat
     1141 atgttctacg gcgaagcgga ctacgaaatt ccgcggccga aaaagatcgc ccgcggccct
     1201 ggcgccgacg agtctctgga cgaagccgcc gagctgctgg cgagcgccaa attcccagtg
     1261 atggtcgctg gtggcggcgt aatcttctcg ggaggcatcg aggaggccaa agcgcttgcc
     1321 gagtacctgc agatgcccgt cgtcaatagc tatctgcaca acgacagctt cccggctgat
     1381 catccgctct ggtgcggccc gctcggctac cagggctcca aggcggccat gaagatcatc
     1441 gctgaggcgg acgtcgtgct cgcactcggt acgcgactcg ggccgttcgg caccctgccg
     1501 cagtacggcc tcgactactg gcccaggaat gcgaagatca tccaggtcga cactgaccac
     1561 cgcatgctgg gccttgtgaa gcagatctcc gtcggcatcg tcggggatgc gaaagcggcc
     1621 gccagagcat tacatcatcg cctcgccaac cgcgatcccg ttgcgcgccg gaacgcctcg
     1681 gagcggttgg agaagatcaa ggcggagaag gacgcttggg aaaaggagct ctcagagtgg
     1741 acgcatgagc gcgatccctg gagcctcgag gtcgcgaagg actctcctta catgcacccg
     1801 cgccagatgc tgcgcgagct cgagaaggcc ttgccgaaga acgccatggt ctcgaccgac
     1861 atcggcaaca tttgctcggt ctcgaacagc tatctgcggt tcaaccaggc accgagcatg
     1921 ttcgcggcca tgagcttcgg caactgcggg tacgccctgc cgaccatcat cggcgccaag
     1981 gtggcctgcc cggatcgccc ggccatcgcc tatgtcgggg acggcgcctg gggcatgagc
     2041 ttcggcgaga tcctcacctg cgtgcgcgag aacattcccg tcaccgctgt cgtcttccac
     2101 aacaagcagt ggggcgcgga gaagaagaac caggtggact tctacaatcg ccgcttcgag
     2161 ggcgtggacc tcaagaaccc gtcctgggcg gaggtcgccc gctcgctcgg cgccgagggt
     2221 gtcactgtca gcaacctcga acaggtgggc gaggcgctcg aaaaggcggt cgatgcccag
     2281 atgaagcagg gcaagacgac cgtcatcgag atgatggtga cgcaggagct tggcgatccc
     2341 ttccgccgtg acgccctgag gaagccacgg cgcctgctgg agaagtacaa gcacaccaac
     2401 gtggagtaat gccattcagg cagagcccgc ggctgtgttg ctacccgtcc ggaccctgtt
     2461 cggagttgtc acgcagagtg gcagccagcc gacctttcgg cgcgaacggg ggatgggtga
     2521 cgtggtcgcc
//
LOCUS       DASUMG010000373         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_49102, whole genome shotgun sequence.
ACCESSION   DASUMG010000373 DASUMG010000000
VERSION     DASUMG010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49102"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFX46_06125"
     CDS             <1..1486
                     /locus_tag="VFX46_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600191.1"
                     /translation="CYPRKLKGRMRRKPHRACGRRALISMRPVSRLLPVVMLLPVISA
                     GTPSSAPGVSTPQPALDQSLALSSSKTESYGVLRLAQNAPVVHVARVFVMEPGAEIPL
                     PIQVEAMPRNSFLRLRGLPANATLSEGHQIGPGAWAVPLVSLPNIRVRVPRETTGRFE
                     VTISAVNVAGDILSEIKTNLVVASSSSLLAGISEQPAGEAAPSSPQPPAPTLAERAGL
                     GQPSTPDAPATGMAGQPPLSIASAADIKPPSAKNCGAAEISTAALPAAHMAINIKATS
                     CRANEPVTIEYAGARFVRQLDQAGELNFTLDCFAGTSSKIVITFADGARGAVDVTTRD
                     LGRVSKVAVIWQAPVNLDLHAFEYAASFGEPGHVWAASPSSPNDAWEKTLATGRGHGF
                     ITAADGNAEGDKIEVYTLWHHEEQTSGAIEMAVDFESRGDTPSGDMCGNGPLSQVAFE
                     VVMLSRHGEVTRQQAMMLPMECGVTLTQSARYNKSVIPVLRIRR"
     gene            1512..3122
                     /locus_tag="VFX46_06130"
     CDS             1512..3122
                     /locus_tag="VFX46_06130"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HEX5600192.1"
                     /translation="MKAISVAGAFRTVGLALMLAAIASAGAEATEVTLRLKGGGFQLS
                     GELKEYDTNKYVIENKSFGTMTLDASRFDCIGQGCPRAPFAAPASLSLPRGVSRTIRI
                     HGSDVIGAELMPLLVQGYANSIGAKAVKAVGANPLEMQIRLTDANDREITLVELHRYG
                     TDQAFQGLANGSVEIVMADRPITEEELAMLARAGLSDMRSPAHEHVIALDGLVVVVSE
                     DNPAVSLSIENVARIFAGQLTDWSEIGLPPGRINVYATESRSGTFSVFDALVLKPRNL
                     TLTREAQIIDSNSGVSDAVARDPNGIGLTSFAFLRNAKAINIESSCGLITRPSVFTVK
                     TEEYPLTRRIYLYSGILTNPHARGLIDYIFSPAAQSQIAAAQFVDQTPDLLGFEDQGG
                     RIAYALNAPSEDFDMAMMRQLISDMNGAQRMSITFRFRLGSVDLDARARADIDRLIEV
                     MRTPEFSNKSILLLGYADSVGQFSSNSVLSQRRATQVHETLLAAAGNTINPALISSRG
                     YSELAPVACNDSEEGRNLNRRVEVWVRE"
     gene            3257..>3792
                     /locus_tag="VFX46_06135"
     CDS             3257..>3792
                     /locus_tag="VFX46_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5600193.1"
                     /translation="MRSICRRVVHFAVATILCSFLIGLPAVAQWQPSKPIDFVIMAGK
                     GGGADRIVRNIIEIIEKHRLSPVPMNPVNIPGNSGADALVHLKQRTGDNHVLLFTLNS
                     FFTTPIERPELGIDISTFAPIGRMAEDTFLLWIHADRTDIKSLDDFVKAARAKGEKWI
                     MAGTGQGQEDSLLTDFLNS"
BASE COUNT          781 a         1170 c         1137 g          704 t
ORIGIN      
        1 ttgctacccc cgcaagctca agggtcggat gcggagaaag ccccatcgcg cctgcggtcg
       61 tcgtgcgctc atcagcatgc ggccggtaag ccgcctcttg cccgttgtta tgctgctgcc
      121 ggtcatatcg gccggcacgc cttcgtcagc acccggggtc agtacacccc aaccggcact
      181 ggaccagtct ctcgcattga gcagttcgaa aacggaaagc tatggcgtcc tgcgcctcgc
      241 gcagaatgct cctgtggtcc acgttgcccg cgtgttcgtg atggagccgg gagctgagat
      301 cccgcttccc atccaagtgg aggccatgcc gcgcaacagc ttcctccgcc tgcgcggcct
      361 gccagcgaac gcgaccttgt cggaaggcca tcagatcgga ccgggtgcct gggcggtccc
      421 gctggtatcg cttccgaaca ttcgcgtccg ggttccacgc gagacgacgg gccggttcga
      481 agtcaccatc agtgctgtga acgtggcggg ggacatcctg tccgaaatca agaccaattt
      541 ggtggtcgcc tcctcgtcat ctctgctggc tggaatatcc gagcagccag caggggaagc
      601 ggctcccagt tcgccgcaac cgcccgcacc cactttggct gagcgagccg gcctcgggca
      661 gccgagcacc ccggatgctc cggcgactgg aatggctggg cagccaccgc tctcgattgc
      721 gagcgccgcc gatatcaaac ctccctcagc caagaactgt ggcgccgccg aaatctcaac
      781 ggcagctctg ccggcggccc atatggccat caacatcaaa gcaacgagtt gccgcgcgaa
      841 tgagccggtg actatcgagt acgcaggcgc gagattcgtg cgccagctcg atcaagcggg
      901 tgagctgaat ttcacgctgg attgcttcgc agggacgagc tcgaagatcg taatcacatt
      961 tgccgacggc gctcggggtg ccgtcgacgt gaccactcgt gatctgggcc gcgtgtccaa
     1021 agtcgctgtg atttggcaag cccccgtcaa cctcgatctt cacgctttcg aatacgcagc
     1081 ctctttcgga gaaccggggc acgtctgggc cgcatcgcct tcctcgccga atgatgcgtg
     1141 ggagaagacg ctggccaccg gccgcggaca cgggttcatc accgctgccg acgggaatgc
     1201 cgagggcgac aaaatcgagg tctacaccct ctggcatcac gaggagcaga cctcgggtgc
     1261 catcgagatg gcggtcgact tcgagtcccg cggcgatacg ccaagcggcg acatgtgcgg
     1321 aaacggaccg ctgtcccaag tggcattcga ggtcgtgatg ctgtcacgcc atggggaggt
     1381 gacacggcag caggcgatga tgctgcccat ggaatgcggc gtcaccctaa cgcagtcggc
     1441 gcgatacaac aagtcggtca tccccgtatt acggatccgg cgatgaccgc gcatcggggg
     1501 gacaaagccc aatgaaggca atcagtgtgg cgggagcatt tcgcaccgtt ggtttggcgc
     1561 tgatgctcgc agcgatcgcg agcgccgggg ccgaggcaac cgaggtgacg cttcgcctga
     1621 aaggcggcgg gtttcagctc agcggcgagc tcaaggagta cgatacgaac aagtacgtga
     1681 ttgagaacaa gagcttcggc acgatgacgc tcgatgcgtc gaggttcgat tgcatcgggc
     1741 aaggttgccc ccgtgctcca tttgccgctc ctgcctctct cagtctcccc cgcggggtgt
     1801 cacgaacgat ccggatccac ggctctgacg tgatcggcgc cgagctgatg ccccttctcg
     1861 ttcagggcta tgccaacagc attggcgcca aagccgtcaa agcagtgggg gctaatcccc
     1921 tcgaaatgca gatcaggctg accgatgcga atgaccgcga gataaccctg gtggaactgc
     1981 accggtacgg cacggatcaa gcgttccaag gtttggccaa cggctccgtg gaaattgtca
     2041 tggcagaccg gccgatcacg gaggaggagc tcgccatgct ggcaagagcc ggtctgagcg
     2101 atatgcgctc ccccgcgcat gagcatgtca tagcattgga cggcctcgtt gtcgtcgttt
     2161 ccgaggacaa tccggcagta tcgctttcta ttgagaatgt cgcccgtata ttcgccggac
     2221 agctgaccga ctggtccgaa atcggactgc cgccgggtcg cataaatgtg tatgccacag
     2281 aaagccgctc cggcacgttc agcgttttcg atgccctcgt cctcaagccc agaaacctca
     2341 cgctgaccag agaggcgcag atcatcgact cgaacagcgg cgtttcggat gccgtcgccc
     2401 gcgacccgaa cggcatcggg ctcacgtcat tcgccttttt gcgcaacgcg aaggccatca
     2461 acattgaaag ctcgtgcggc ctcatcacga gaccgtccgt tttcaccgtg aaaacggagg
     2521 agtacccgct gacccgccgt atttatcttt acagcgggat tctgaccaac ccccacgcac
     2581 gcgggctcat cgactacatt ttctcgcccg cggcgcaaag ccagatcgca gcggcccagt
     2641 ttgtcgatca aacgccagat ctcctgggct tcgaggacca gggtgggcgt atcgcctacg
     2701 cgctcaacgc acccagcgaa gacttcgaca tggcaatgat gcgccagctc atctccgaca
     2761 tgaacggggc gcagcgcatg tcgatcacat tccgctttcg gctgggatcg gttgatctcg
     2821 atgcaagagc gcgcgccgat atcgaccggc tgatcgaggt catgcggacg ccagagttct
     2881 ccaacaagtc cattttgttg ctggggtacg cagactccgt cgggcaattc tcatccaaca
     2941 gcgtgctctc gcagagaaga gccacacaag tgcacgaaac tctactggcc gcggctggaa
     3001 acacgatcaa tccagcgctc atctccagca gaggctacag cgagctcgca cctgtcgcct
     3061 gcaacgactc tgaagaaggc cgcaacctca accgaagggt cgaagtgtgg gttcgagaat
     3121 gatcgcagat gggagtggat tccggaaggg gtgccggatc ggcgctgcgc ctctcaccga
     3181 aaatggatga gctggcggac atcatagctc gaggcggagc aaatctctgg atccgatcag
     3241 cagggtgatc ttcaacatgc gttcaatctg ccgtcgagtc gtccactttg ctgtggcgac
     3301 gatcctatgc tcgttcctga ttggtctgcc cgctgtggcg caatggcagc catccaaacc
     3361 gatcgatttc gtgatcatgg ccggcaaggg cggcggagcg gaccgtatcg tccgcaacat
     3421 aatagagatc atcgagaagc accggctttc gcccgttccg atgaacccag tgaacattcc
     3481 ggggaattcg ggggccgatg cgttggtcca tctcaagcaa aggacaggcg acaatcatgt
     3541 cctgctgttc acgctgaaca gcttctttac gacgccaatc gagaggccgg agcttggcat
     3601 cgacatcagc acattcgcac cgatcggaag gatggcagag gacacgttcc tcctctggat
     3661 ccacgccgat cgcacggaca tcaaatcgct cgacgacttc gtcaaggcgg ccagggccaa
     3721 aggtgagaaa tggatcatgg ccggaaccgg ccagggtcag gaggacagcc tgctgacgga
     3781 tttcctgaac tc
//
LOCUS       DASUMG010000374         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_531222, whole genome shotgun sequence.
ACCESSION   DASUMG010000374 DASUMG010000000
VERSION     DASUMG010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531222"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFX46_06140"
     CDS             complement(<1..881)
                     /locus_tag="VFX46_06140"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018786 - haloalkane dehalogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX5600194.1"
                     /translation="MHTHIDQHHVAALDSTMAYREVGASGRAVALFLHGNPTSSFIWR
                     NIMPQVAPVARCIAPDLIGFGQSGKPDIEYRFFDHARYLDAFIEALHIDQFFLVAQDW
                     GTALAFHFAARRPAQVCGLAFMEFIRPLPSWEDFHQSPVARATFRKFRTPGEGEAMIL
                     EGNAFIERVLPGSIKRRLSDEEMAAYRNPFLTPESRRPIWRLPNELPIEGKPADVHAA
                     LEAAHAALAVADYPKLLFVGDPGALVSPAFAERFAASVKNCQVVPLGPGLHYLQEDHA
                     EAIGRSIAEWISEIEATR"
     gene            1117..>2423
                     /locus_tag="VFX46_06145"
     CDS             1117..>2423
                     /locus_tag="VFX46_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX5600195.1"
                     /translation="MPSTRAFAMHLGVSRNTALASYDLLLSEGYLTSRPGAGTFVADL
                     RPRQNPRKSRATEAGGDGRLAAFWRGSARSLRPRPATTPRFDFRVGLPDRSLLPLQVW
                     RRVTARALRTLSRSPTAYAEPEGRHVLRDAISKHVSFARLVACQADDIVVTAGAQQAF
                     DLIARILVTPGKTTVAVEEPGYPPLRAAFAAAGAKLVTVAVDSEGLVVDQLPSTTRVI
                     CVTPSHQFPLGIVMSPRRRMALLDFAQSHDAAVIEDDYDSEFRYDGRSLDALKTLDRT
                     DLVFYVGTFSKSLFPEIRLGFVVVPKWAQPALVEAKRIADWHGTVFAQEALAIFIDEG
                     HMARHVRKARTVYGRRRGELLRALADHCGKQLQPMGGESGLHLAATLLTPVGARVVAA
                     SAAEIGIEVTPLDAFAQSSRAPNGLAFGYGMIGEKQIDEGVRGL"
BASE COUNT          443 a          763 c          768 g          449 t
ORIGIN      
        1 cgcgtggctt cgatctcgga aatccattca gcgatcgacc ggccgattgc ctcggcgtga
       61 tcttcctgca aatagtgcag accgggtcca agcggcacga cttggcagtt cttgaccgaa
      121 gcggcgaagc gttcggcaaa ggctggcgag accagcgcgc ccggatcacc aacgaacaac
      181 agtttgggat aatccgccac ggccagcgcg gcatgcgcag cttcaagcgc ggcatggacg
      241 tcggccggct tgccctcgat cggtagttcg tttgggagcc gccatatcgg ccgccgggac
      301 tctggcgtga gaaacgggtt gcgataggcg gccatctcct cgtcgctgag cctgcgcttg
      361 atcgagccgg gaagcacgcg ctcgatgaat gcattgcctt ccagaatcat cgcttcgccc
      421 tcgcccggcg ttcggaactt gcggaacgtc gcgcgcgcca ccggagactg gtgaaaatcc
      481 tcccagctcg gcagcggccg aatgaactcc atgaaggcga ggccgcacac ctgcgcgggg
      541 cgccgcgccg cgaagtgaaa tgccaacgcc gttccccagt cctgggccac gagaaagaat
      601 tgatcgatgt ggagagcttc gatgaatgcg tcgaggtatc gcgcgtgatc gaagaagcga
      661 tactcgatat ctggctttcc cgactgccca aagccgatca agtcgggcgc aatgcagcgc
      721 gccacgggtg ccacctgcgg catgatgttg cgccagatga acgacgaggt cggattgccg
      781 tgcaggaaga gcgcgacggc ccggccactc gcccccactt ccctgtaagc catggtcgaa
      841 tcgagagcgg cgacgtgatg ttggtcgata tgcgtgtgca tccttgcgtc ctcgcgaatg
      901 cgcgggtcgt cgggccctgc ccagcgatcc ctggatgcat tgaatcatgc cgagacgagt
      961 ggtactaaaa aagtaccaat cttggaaaaa aacatggagc cagttctgga gcttccgatt
     1021 cgccttcccg gcaggggatc gcgacgcctg cttcggaatt tgcatgagca gctaagggcc
     1081 gcgatccttg acgggcggct gcagcccggc cttcgcatgc cgtccacgcg agcatttgca
     1141 atgcatctgg gcgtgtcgcg caacacggcg cttgcgagct acgacctctt gctcagcgag
     1201 ggctacctga cctcgcgtcc gggcgccggt acttttgtgg ccgacctgcg tccgcgccaa
     1261 aacccgcgca agagccgcgc cacggaagcc ggcggagacg ggcgcctggc cgcattctgg
     1321 cgcgggtcgg caagatcgct acgcccaagg ccagccacaa caccgcgttt tgatttccgg
     1381 gtcggtcttc ccgaccggtc gttgcttccc cttcaggtct ggcggcgcgt gaccgcgcga
     1441 gcgctcagga cgctgtcgag gtctccgaca gcctacgccg aacctgaagg ccggcatgtg
     1501 cttcgcgacg ccatctccaa gcacgtttca tttgcaaggc tggtggcttg ccaagccgac
     1561 gatatcgtcg tcaccgccgg cgcccagcag gccttcgacc tgattgcgcg gatactggtg
     1621 acgcccggga agaccacggt agccgttgaa gaaccgggct atccgccgct gcgggcggct
     1681 ttcgctgcgg ccggcgcgaa gctggtcacc gtcgccgtcg actcggaagg cctggtcgtg
     1741 gatcaactgc catctacgac gcgcgtcatc tgcgtgacgc ccagccacca gttccccctg
     1801 ggcatcgtca tgtcgcctcg ccgccgtatg gctttgctcg attttgccca atcgcacgac
     1861 gcggctgtta tcgaggacga ctacgattcc gagttccggt atgacggccg gtcccttgat
     1921 gcgttgaaga cgctcgatcg aacggacctc gtcttctacg tggggacatt ctcaaaaagt
     1981 ctgtttcccg agatcaggct cggcttcgtc gttgtcccca aatgggcgca gccagccctc
     2041 gtcgaagcca agcggatcgc cgactggcat gggaccgtgt tcgcacagga ggcactcgca
     2101 atattcatcg acgaaggtca catggcccgc cacgttcgaa aggcgcgaac ggtctatggt
     2161 cgccgccgcg gcgagctgct tcgcgccctg gcggatcact gcggcaagca gttgcagccc
     2221 atgggaggag agtcgggcct gcatctggcg gcgacgctgc tgacgcctgt cggcgctcgc
     2281 gtcgttgccg caagcgctgc ggaaatcggc attgaagtaa cgccgcttga cgcgttcgcg
     2341 caaagctcgc gggctccgaa cggattggcg ttcggatatg gcatgattgg cgaaaaacag
     2401 atcgatgaag gtgtgcgtgg tct
//
LOCUS       DASUMG010000375         4088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_771519, whole genome shotgun sequence.
ACCESSION   DASUMG010000375 DASUMG010000000
VERSION     DASUMG010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771519"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            499..2127
                     /locus_tag="VFX46_06150"
     CDS             499..2127
                     /locus_tag="VFX46_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX5600196.1"
                     /translation="MVQPSEPTTHEIVTDLKRQVFSVCRPGRPDRCQPMMVYQFEVAC
                     GSVHVPWMDLATALLSGSGTSAWIEGERLHIRPRSVRPRLGDCADGDPTTGFMRKCEP
                     WELDQLGAEWVLPPGFAPVHEVGAKFVDLPPPAPPPLPEPRVTAFDSEQLQTPSESAA
                     VPPSASADAERLWAHEHATPPDTQEDIARTQPSPEAEAAYEPYDAAESAMSSPGGSQS
                     TASIEHRDPSAFVRNVSEAIAAAASAEPEAAADAAEPEATSSVRGWSAMLEFASSKAV
                     TWLSDSFLSAFEPLAAALKDPKLENQVTAALMGIALLSCLVSGIGWYSVRARRARAPA
                     FRVSADGRQPIVHTFRPPSKREAQSQDGADQTSGTALVAPTDEKICGELCRTAHNLLQ
                     QIEVRVDDLQGVAPLRRVLQREMRSLEQFLTAVMTASPQEPEEWRRMRNRLQRIVREL
                     HRLRDIVEGAYRSLSAGGFTSREPPRDKYEAYEALGVNPDVDLRTLKKLVDALRACWH
                     PDLAKDPTDRANREERMKRINIAWDIITSKRQEA"
     gene            complement(2257..2763)
                     /locus_tag="VFX46_06155"
     CDS             complement(2257..2763)
                     /locus_tag="VFX46_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX5600197.1"
                     /translation="MVFTRKPEPRSGNIDPLQIKTMDQATGTPTPTPIRGNSYSGTSG
                     QDANESVIGNDLTIEGQAITIRCQGSLRVNGNIQADLHSMKLTVGEEAVISGTIAAET
                     VDVYGKVQGAILGSRVVLHSSAVVEGDIHSQALAIDQGASFDGRSRKVKDPSEIAPQL
                     TSPNRSNS"
     gene            complement(2889..>4088)
                     /locus_tag="VFX46_06160"
     CDS             complement(2889..>4088)
                     /locus_tag="VFX46_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5600198.1"
                     /translation="FQMRAFEDRFMTLGLPYRVIGGPRFYERQEIKDAIAYLEVTLNP
                     ANDLKFERIINVPKRGLGDTTIKRIHALARATDMPLYHAARSITETEEITGKARKSLI
                     ELINSFERWRGLVPSMKHTELAELILDESGYTAMWQQDKSPQAQSRLENLKELIRFMH
                     EFESLAGFLEHVSLVMDADQTDTDDRVSLMTLHAAKGLEFDVVFLPGWEEGLFPHQRS
                     LDENGQAGLEEERRLAYVGLTRAKRQVFVSFAQNRRTRGLYQAAIPSRFVDELPPEHV
                     DVVEAPSRFGGAYASFDRFANPYGRSRFDDAGGSFQTNYQTPGWQRAQQRWRQGERPN
                     RGPLMIEGELVASSTGRPSSFQEGERVFHQKFGYGQIAEVDGNKLTVDFDKAGRKRVV
                     DSFVDRV"
BASE COUNT          762 a         1289 c         1254 g          783 t
ORIGIN      
        1 gaacgagatc cgatgctaaa cggcaactgg tttcctgtag tgcggtagcg ccttagagcc
       61 gcctagtcga tcgggcaaac cgtcgggccc tcatgttggc tccatgagcc gaggccggag
      121 ccaatctcgg gcgcaagggt ccgctgcggc cgcccccgtt caaaccgaaa tcgtcaagta
      181 tttcaacaat atagtttttt gatgcctttg tggtgcagac gaaacgatta aggttttcta
      241 aagcaaggac ataagaattc gactgcagcg gcgggacgcc ccgataaaat ggtggggatg
      301 tcacagtcat gccgctcttg ccagataatc ggcatgctgt cgtacctgca tgggggctgc
      361 taggtgcatc tacgcttggt tccgcacctt attgtgttgg cagtgtccgc gttactgctc
      421 ggatcgggtg cggacaaggc gctcgcacgc cgaggcgtct tgctgcccgc cctttgcgat
      481 catcgcgacg gccgcctggt ggtacagcct tcggagccga ccacccacga gatcgttacg
      541 gacctgaagc gccaggtgtt ctcggtttgc cggcctgggc gtcccgaccg ttgccagccg
      601 atgatggtct accagttcga ggtggcctgc ggctccgtgc acgtgccgtg gatggacctc
      661 gcgacagccc tcttgtccgg aagcggaact tccgcgtgga tcgagggtga gcgactccac
      721 atcagaccgc gttcggtgcg gccgcgcctg ggcgattgcg ccgacggcga cccgacgacc
      781 ggcttcatgc ggaagtgtga gccctgggag ctggatcagt tgggagcgga atgggtgctg
      841 ccgcccggct ttgcgccggt tcacgaggtc ggagccaagt ttgtcgactt gcccccaccc
      901 gccccgccac ctttaccaga gccgcgggta accgctttcg atagtgaaca gcttcagacc
      961 ccaagtgagt cagcagcggt cccacccagc gcatctgccg acgctgagcg cctctgggcg
     1021 cacgaacacg ctacgcctcc tgacacccaa gaagacatcg cccgtacaca gcccagcccg
     1081 gaagccgagg cagcctacga gccgtacgat gctgctgagt ccgccatgtc ctcacctggg
     1141 ggcagccaga gcacagccag catcgagcac agggacccca gcgctttcgt acgcaacgtt
     1201 tcggaggcca tcgcagccgc tgcatcggcc gagcccgaag ctgccgccga cgctgctgag
     1261 ccggaggcca ccagctccgt tcgcggctgg tcggcgatgc tggagttcgc gagcagcaaa
     1321 gccgttacct ggttgtcgga ctcgttcttg agcgcgttcg aacccctagc cgccgctctc
     1381 aaggacccga aactggagaa tcaggtcacg gcggcgctga tgggcattgc ccttttgtcc
     1441 tgcctggttt ccggaatcgg ctggtactcg gtgcgggctc gccgcgccag ggcgcccgct
     1501 ttcagggtaa gcgccgatgg aaggcaacct attgtccaca ccttccggcc gccgagcaag
     1561 agggaggccc aatctcagga cggcgcggac caaacctcag gtacggccct tgtcgctccc
     1621 acggacgaga agatctgcgg agagctgtgt cgcacagcgc acaacctgtt gcaacagatc
     1681 gaggtccggg tggacgacct gcagggcgtg gcacccctcc gtcgcgttct tcagcgcgag
     1741 atgcgcagct tggaacagtt cctgacggct gtgatgaccg cctctcctca agagcctgag
     1801 gaatggcggc ggatgcgtaa ccgcctgcag cgaattgttc gggagctgca tagacttcgc
     1861 gatatcgttg aaggagccta ccgcagcctc tctgcgggcg ggttcaccag ccgcgagccg
     1921 ccgcgggaca agtacgaggc ctacgaggcg cttggcgtca atccggatgt cgatctgcgg
     1981 acgctgaaga agctcgtgga cgcgctacgc gcctgctggc atccggatct cgcaaaggac
     2041 ccgaccgacc gggcgaaccg cgaggagcgc atgaagcgga tcaacatcgc ctgggacatc
     2101 atcacgtcga agcgccaaga ggcgtgacct cctgcacagc ccaagtcacg cccccgtaaa
     2161 gaagtgacga gcgctacgcg cttctatttt gacgtctcag atacaaacag gccggacggt
     2221 gtccggcctg ttaggccttg ttccgacgaa atcgtctcag ctatttgacc gattggggga
     2281 ggtcagctgc ggcgcaatct cggacggatc cttcaccttg cgggagcggc cgtcgaagct
     2341 tgcgccctgg tcgatagcga gggcctggct gtggatgtca ccttcgacca cggcgctgga
     2401 atgcagtaca acgcgcgagc ccaggatcgc accctgaact ttgccgtaca cgtcgaccgt
     2461 ctccgcggcg atggtgccgc tgatgaccgc ttcctcaccg accgtcagct tcatgctgtg
     2521 caggtcagct tgaatattgc cgttgacgcg caacgaaccc tggcaacgaa tcgtgatcgc
     2581 ctgaccctcg atcgtgaggt cgttgccgat aacggattcg tttgcgtcct gcccggacgt
     2641 gcccgaatag ctattgccac gtatcggggt tggggttgga gtgcctgtcg cctgatccat
     2701 tgtcttgatc tgcagcgggt cgatgttgcc ggacctgggc tcgggcttgc gtgtgaagac
     2761 cattactctg aactcccctc gagtactaat tccggcgcga gcatccgctc cggaggtggt
     2821 cgaattgtgt cattcgacca cggaactcaa gtccgaagct tcagccggca gacttacggg
     2881 agtaccgttt aaactcgatc gacgaagctg tcgacaacac gtttgcggcc cgctttgtcg
     2941 aagtcgaccg tcagcttgtt tccatcgacc tcggcaatct gaccgtaccc gaatttctgg
     3001 tggaagaccc gctccccctc ttgaaatgac gacggccgac cggttgagga ggcgacaagc
     3061 tccccctcga tcatcagtgg tccacggttc ggccgttcgc cctgtcgcca gcgctgctgc
     3121 gcccgctgcc agcccggcgt ttggtagttg gtctgaaaac ttccgccggc atcatcgaaa
     3181 cgactgcgcc catagggatt ggcgaaacga tcgaagcttg cgtaggcacc gccgaacctg
     3241 ctcggcgcct ccaccacatc gacgtgctca ggcggcagct catcgacgaa gcgagacgga
     3301 atggccgctt ggtacaatcc ccgtgtgcgg cggttctgcg cgaacgaaac aaacacctgc
     3361 cgcttggcgc gggtcagccc gacataggcc agccgccgtt cttcctcgag gccggcctgg
     3421 ccgttctcgt ccagagagcg ctgatggggg aacaatccct cctcccagcc aggaaggaag
     3481 accacgtcga actcgagccc cttggcggcg tgtagcgtca tcaacgacac gcgatcgtcg
     3541 gtgtcggtct gatccgcgtc catgacgagc gagacgtgct cgaggaagcc agcgagcgat
     3601 tcgaactcat gcatgaagcg gatcagctcc ttcaggttct cgagccgcga ctgcgcctgg
     3661 ggcgacttgt cctgctgcca catggccgtg tagccggact cgtcgaggat cagctctgcc
     3721 agctccgtgt gcttcatcga gggcacgagc ccccgccagc gctcaaatga attaatcagc
     3781 tcgatgagcg acttgcgcgc cttgccggtg atctcctccg tctcggtgat cgagcgcgcc
     3841 gcgtgataaa ggggcatgtc cgtcgcgcgg gcgagcgcat ggatgcgttt gatggtggta
     3901 tcacccaacc cgcgcttcgg cacattgatg atgcgctcga acttcagatc attggcggga
     3961 ttgagcgtca cctcgagata ggcgatcgca tccttgatct cctgacgctc atagaaacgc
     4021 ggcccgccga tcacccggta cggcaggccc agagtcatga accggtcctc gaatgcgcgc
     4081 atctgaaa
//
LOCUS       DASUMG010000376         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_370921, whole genome shotgun sequence.
ACCESSION   DASUMG010000376 DASUMG010000000
VERSION     DASUMG010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370921"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1659)
                     /gene="thiC"
                     /locus_tag="VFX46_06165"
     CDS             complement(<1..1659)
                     /gene="thiC"
                     /locus_tag="VFX46_06165"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX5600199.1"
                     /translation="MNKITRKSELMVPKVTTGPINASKKVYDSPDGYPDLRVPFREIA
                     LTDPEQPTFRVYDSSGPYTDENARIDVEKGLPRIREAWILERGNVEQYEGRPVRPEDN
                     GNVSGRLLARDFPNKSKPYRRVSDDKPLTQLEYARAGIITKEMIYVAHRENLGRKAAL
                     ERAKAALKDGESFGASIPEFITPEFVRDEIARGRAIIPANINHTELEPMIIGRNFLVK
                     INANIGNSAVTSSVEEEVEKMVWAIRWGADTVMDLSTGRNIHNTREWIIRNSPVPIGT
                     VPIYQALEKVGGDPVKLDWEMFRDTLIEQAEQGVDYFTIHAGVRLAYIPLTANRVTGI
                     VSRGGSIMAKWCLAHHRESFLYERFGDICDIMRAYDVSFSLGDGLRPGSIADANDAAQ
                     FAELETLGELTKIAWAKGCQVMIEGPGHVPMHKIKINMDKQLAECGEAPFYTLGPLTT
                     DIAPGYDHITSGIGAAMIGWFGTAMLCYVTPKEHLGLPDRDDVKTGVITYKIAAHAAD
                     LAKGHPAAQLRDDALSRARFDFRWEDQFNLGLDPDTARRFHDETL"
     regulatory      complement(1755..1867)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2137..2406
                     /locus_tag="VFX46_06170"
     CDS             2137..2406
                     /locus_tag="VFX46_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HEX5600200.1"
                     /translation="MVDAGFAAMLRGYSLTTAEILYRMPDHPSLLQTFVWQDYDLAPE
                     FPKLVKFLDFWSKNLDGKLYRVRVAHRRLIHPSEVRLVNGELRLH"
BASE COUNT          424 a          746 c          783 g          534 t
ORIGIN      
        1 cagggtctcg tcgtggaagc gccgggcggt atccggatcg aggcccaggt tgaactggtc
       61 ctcccagcgg aagtcgaacc gggcgcggct gagcgcgtcg tcccggagct gcgcggcggg
      121 atgccccttg gcgaggtcgg cggcgtgggc ggcgatcttg taggtgatga cgccggtctt
      181 cacgtcgtcg cggtcgggca ggccgaggtg ttctttcggc gtgacgtagc agagcatggc
      241 ggtgccgaac cagccgatca tggcggcgcc gatgcccgag gtgatgtggt cgtagcccgg
      301 cgcgatatcg gtggtgagcg gcccgagcgt gtagaacggg gcctcgccgc actcggccag
      361 ctgcttgtcc atgttgatct taatcttgtg catcggcacg tggccggggc cctcgatcat
      421 cacctggcag cccttggccc aggcgatctt cgtcaactcg cccagcgtct ccagctcggc
      481 gaactgggcc gcgtcgttgg cgtcggcgat ggagccgggg cgcaggccgt cccccagcga
      541 gaagctcacg tcgtatgccc gcatgatgtc gcagatgtcc ccgaagcgct cgtagaggaa
      601 gctctcccta tgatgagcaa ggcaccactt ggccatgatg gagccgccgc gtgagacgat
      661 gcccgtcacg cgattggccg tgagcgggat gtaggcgagc cgaacgccgg catggatggt
      721 gaagtagtcg acgccctgct cggcctgctc gatcagggtg tcccggaaca tctcccagtc
      781 gagcttcact ggatcgccgc ccaccttctc cagcgcctga tagattggca ccgtgccgat
      841 gggcaccggg ctgttacgga tgatccattc gcgcgtgttg tggatattgc ggccggttga
      901 gaggtccatc accgtgtcgg cgccccagcg gatcgcccac accatcttct cgacctcttc
      961 ctccacggag gacgtgacgg ccgagttgcc gatgttggcg ttgattttca ccaggaagtt
     1021 tcggccgatg atcatcggct cgagctcggt gtgattgatg ttcgccggga tgatagcccg
     1081 gccgcgggcg atctcgtccc gcacaaactc cggcgtgatg aactccggaa tggaagcccc
     1141 gaagctctcg ccatccttta gcgcggcttt ggcccgttcg agcgccgcct tccgcccgag
     1201 attttcgcgg tgggcaacat agatcatctc cttcgtgatg atcccagcac gtgcgtactc
     1261 gagctgcgtc agcggcttgt catcggaaac gcgccggtac ggcttcgact tgttggggaa
     1321 atcccgagcc aacaaacggc cgctgacatt gccgttgtct tcggggcgta cggggcggcc
     1381 ttcatactgc tcgacattgc cgcgttctag aatccatgcc tcgcggattc gcggcagccc
     1441 cttctcgaca tcgatgcgtg cattctcgtc ggtatagggg ccggatgagt catagacccg
     1501 gaatgtcggc tgctccggat ccgtcaacgc gatctcccga aatggcaccc gcaggtccgg
     1561 ataaccgtcg ggagagtcat agaccttctt gctggcattg atcggcccgg tggtgacctt
     1621 gggcaccatc agctcgctct ttcgggtgat tttgttcatc tcttcactcc cggatcggcg
     1681 cgcgtgtcgt tcgctgacga tgcgcgcaaa cgctcatcgc tcgatatccg gaatgcgtcg
     1741 gtgaaggagg gggttcttgc gtacgtgtcc gtcccttcgc cggcattacc cggatcaggt
     1801 tcaaagggtg gcggcgcggc atcagcgcca tctcagccgg ctgccgccgg cccccctcgg
     1861 aacgcaggca gtctgcgacg ggttggctcg aaaggcaagc tcaaccagac ttggcgactt
     1921 gcagtcggct ttttgcgccg atcaaatctt tggtaagtag gatggtgccc aggttctcgc
     1981 taaagtttta ctcagtaaat acctgaaccc gcactgttag cagcgctgcg aatctgctgc
     2041 tagcatgttg ttttattgtt aaaattcctc ttgccagcca gaaaaaccgg aataccttag
     2101 acttaatctg acaactaatt tggggaaggt cttgcgatgg tcgatgctgg ctttgccgcg
     2161 atgctcagag gctacagtct cacgaccgcg gagattctct accgtatgcc agatcaccca
     2221 tctttgctgc agaccttcgt ctggcaggat tacgatctgg cgccagagtt tccgaagctc
     2281 gtgaagttct tggacttctg gtccaagaat ctggacggca agctttatcg tgtgcgcgtc
     2341 gcacaccggc gcctgatcca tccgagtgag gtccggctcg tgaacggaga gctgcggttg
     2401 cactgagcgt cggccggctg ctcagctgcc gaacccatag gcgtcgcgaa gtaaacgtcg
     2461 acgccgtggc acagacgatg cgcgttg
//
LOCUS       DASUMG010000377         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_371028, whole genome shotgun sequence.
ACCESSION   DASUMG010000377 DASUMG010000000
VERSION     DASUMG010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371028"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VFX46_06175"
     CDS             complement(<1..516)
                     /locus_tag="VFX46_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEX5600201.1"
                     /translation="MAKTRRTWRLWIGSGLVAAALVGIGFLLFAAAATRPPPELPPKA
                     DGIVALTGTQQRIAVAAKLLSTGQAKRLLVTGINRQATRGEIRRLTGLDAALFDCCVD
                     LGYEAQNTIGNAEETSAWIKQHGFSTLIVVTSSYHMPRSLVELGRVMPNVTLIPYPVV
                     SESFRRGDWWMR"
     gene            complement(631..1719)
                     /locus_tag="VFX46_06180"
     CDS             complement(631..1719)
                     /locus_tag="VFX46_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600202.1"
                     /translation="MSGSYDRPAAARRFGHAPDRDEYGYEMADPPTGGQGYPSYETVT
                     QPPATSLRHDYAPPGPIRDPATMPIVPAGSVTGRSLTLVVSIMCFFACLTAGAVYLIN
                     KSAAAWLREIASEVTVQLEPREIPNMESTLNDVVAFLNRQPGVRSARALSVAESNALL
                     EPWLGQIDLLSTLPVPRLVALELDRSNPPDIPALRQRLMAQFKGVTLDDHRHWQQQIR
                     TVTRSFALGGLAILLLVASATTAIIISATRSAMASNREIVEVLHLVGATDRFIAREFE
                     RHFLSLGIRAGLVGAICAMAVFFTMPALMELLGGGTVSMADLRRLVGTGGLDAPGYVL
                     LGFVVVVIAGLCMLTSRFGVYRILHARQ"
     gene            complement(1703..2359)
                     /gene="ftsE"
                     /locus_tag="VFX46_06185"
     CDS             complement(1703..2359)
                     /gene="ftsE"
                     /locus_tag="VFX46_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEX5600203.1"
                     /translation="MIRLENVGLRYGRGPEVLREVNFHLKPGSFHFLTGPSGAGKTTL
                     LKLLFLSLQPTRGKLYLFDQDVSRLTPAKRAQLRRRIGIVFQDFRLLDHLTTWENVAL
                     PLRVVGKRISDYKDDVTDLLQWVGLGDRMHAFPSVLSGGEKQRAAIARAVIGKPELLL
                     ADEPTGNVDPQMARRLLRLFVELNRLGTSVVIATHDHQLMRQFKAPRLELHEGHVRIV
                     "
     gene            2627..3172
                     /gene="hpt"
                     /locus_tag="VFX46_06190"
     CDS             2627..3172
                     /gene="hpt"
                     /locus_tag="VFX46_06190"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX5600204.1"
                     /translation="MSADNDNGRVQVIFSPEEIATRIDALAARIAEQNLERLLVVAIL
                     KGSFVFAADLIRALHKVGLEPEVDFISLSSYRKSTQSSGQVQILRDIEMDVRGRNVLL
                     VDDILESGRTLAFAKDLVAARGAKRVLTCVLLNKPVRRAVQVEADFAAFECPNVFVVG
                     YGMDLAHRYRELPYVGQLATS"
BASE COUNT          624 a          974 c          999 g          583 t
ORIGIN      
        1 cctcatccac cagtcgccgc ggcgaaagga ttccgagacg accggatatg gaataagcgt
       61 cacgtttggc atcacccgcc ccagctcaac caggctgcgg ggcatatggt agctcgacgt
      121 cacgacgatg agcgtcgaaa agccatgctg cttgatccag gcggacgtct cttcggcatt
      181 accaatggtg ttctgcgcct cgtagccgag atccacacag cagtcgaaca aagctgcgtc
      241 gaggcccgtc agccgacgga tttctccgcg ggtagcttgc cggttgatgc ctgtcaccag
      301 cagtcgcttg gcctgtcccg tcgagagcag cttggccgca accgcaatgc gctgttgcgt
      361 gccggtcagg gccacgatgc catccgcctt tggcggcagc tcgggaggag ggcgtgtggc
      421 cgcggcggca aacaacagga agccgatgcc gacaagtgct gccgctacca agccggaccc
      481 aatccagagc cgccaagttc gccgcgtttt agccatggat gaagcttctg gtcgcccgcg
      541 ggcggcctct ctcctgtcct atgcagcatc tatccgtcga gatgcaaacc cgatctctgc
      601 gccggagtcc atgactcttc ggggaacacg ctactgccgg gcgtgcaaga tgcgatagac
      661 gccaaagcgc gacgtcagca tgcataagcc cgcgataacc accacgacga agcccagcag
      721 cacgtaacca ggtgcatcga gcccgcccgt gccgacgagc cggcgaaggt cggccatgct
      781 caccgttccg ccgccaagca attccatcaa ggccggcatg gtgaaaaaca ccgccatcgc
      841 gcaaatcgcg ccaacgagac cagcgcgaat gccgagcgac agaaaatggc gctcgaactc
      901 ccgggcgatg aaccggtcgg tggcgcccac cagatgcagc acctcgacga tctcacggtt
      961 cgaggccatc gcgctccgtg tcgccgagat gatgatcgcc gtggtggcgg atgcaacgag
     1021 cagcagaata gccaacccgc cgagggcgaa ggatcgcgta accgtccgga tttgctgctg
     1081 ccagtgccga tgatcgtcga gcgttacacc tttgaactga gccattaatc tctgcctgag
     1141 cgcaggaatg tcagggggat tgctgcggtc cagctccaag gcgacgagcc gaggcaccgg
     1201 cagagtgctg agcagatcga tctgaccaag ccacggctca agcagcgcgt tggattccgc
     1261 gacgctgagc gcacgagcgc tgcgaactcc gggttgccgg ttcaagaagg caaccacgtc
     1321 attcagcgtg ctttccatgt tgggaatctc gcgcggctcg agctggaccg tcacctcgct
     1381 tgcgatctcc cgaagccacg cggccgcgga cttgttgatc aggtaaaccg cgcctgcggt
     1441 caggcaggcg aagaagcaca tgatcgagac cacgagggtg agcgagcgac ccgtaaccga
     1501 tcccgcggga acgatcggca tcgtggccgg gtcgcggatg ggtcccggag gcgcgtagtc
     1561 gtggcgcaag ctggtcgcgg gaggctgcgt cactgtctca taggatggat agccctgtcc
     1621 tcctgtcggg ggatcagcca tctcatagcc gtactcgtcg cggtcgggag catgcccgaa
     1681 ccggcgtgcg gctgccggtc tatcatacga tcctgacatg cccctcgtgc agctccaggc
     1741 ggggcgcctt gaattggcgc atcagctgat ggtcgtgggt ggcgatgacg actgacgtcc
     1801 cgagccggtt cagctcgacg aagagacgta acagccgccg cgccatctgc gggtcgacgt
     1861 tgccggtagg ctcgtccgcg agcagaagct caggcttgcc gatgacggcg cgcgcgatgg
     1921 ctgcccgctg cttctcccca cccgacaaca ccgacgggaa agcgtgcatg cggtcaccga
     1981 ggcccaccca ttgcagaaga tcggtgacgt catccttgta gtccgagatt cgcttgccga
     2041 ccacccgcag cggcaaggcc acgttttccc aggtcgtcag atgatcgagc aaccggaagt
     2101 cttggaaaac aatgccgatt cggcgcctta gctgggctct ttttgccggg gtcaggcggc
     2161 tgacatcctg atcgaaaagg tagagctttc ctcgcgtggg ctggagcgaa aggaacagca
     2221 gcttgagcag ggtggtcttg ccggcgccgg atggtcctgt caggaagtgg aacgaaccag
     2281 gtttgaggtg gaagttgacc tcccgcagaa cctctggccc tcgcccgtac ctcagcccca
     2341 cattttccaa ccggatcacg tgccgttgtc cccccggatc acttccgcgg cccatttatg
     2401 cgaaaagcga tggggctgat caatcgcagg attgtggcgg gctcgtgtac tttgggctgt
     2461 gcagcaagat tggctgcacc gtccgctgga ggccgaacgc cgttttctac aggaattcag
     2521 ccgctcaaga cctggatgcg gcagcagcag ccctgccctc aggttgcagg ggtgtgctaa
     2581 tcggccgata tttaaggttc tctcagtttc atcccgggga cattggatga gcgcggataa
     2641 cgataacggc cgggtccaag tcatcttctc gcctgaagag attgcgacga ggatcgacgc
     2701 gctcgcggca cgaatcgccg agcagaatct cgagcggctt ctggttgtcg ccattttgaa
     2761 aggcagcttc gtttttgcag ccgacctgat tcgggcgctg cacaaggtcg gcctggagcc
     2821 cgaggtcgat ttcatctcgc tgtcgagcta ccggaaaagc acgcaatcct cgggtcaggt
     2881 gcagatcctg cgagacatcg agatggatgt tagaggccgc aacgtgctgc tcgtcgacga
     2941 cattctggag tcgggccgga cgctggcatt cgccaaggac ttggtggcag cccgtggcgc
     3001 aaagcgggtg cttacttgcg ttttgctcaa caagcccgtc cggcgggctg tgcaggtcga
     3061 ggcggatttc gccgcgttcg agtgcccgaa cgttttcgta gtaggctacg gaatggacct
     3121 tgcgcacagg taccgcgagc tgccgtacgt cggccagctt gccacaagct gaacagtctc
//
LOCUS       DASUMG010000378         3194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_130444, whole genome shotgun sequence.
ACCESSION   DASUMG010000378 DASUMG010000000
VERSION     DASUMG010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130444"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VFX46_06195"
     CDS             <1..546
                     /locus_tag="VFX46_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5600205.1"
                     /translation="LLAGPFLGEWVGWRRLIAILTGFIGILVTIRPGFAAFHPAFLLA
                     LGCMLSYSLYILLTRYLAAYDTSEVTLLYSTLVGTALLAPWAIGDWVWPTNAFTWMVL
                     MSLGFWGGLGHYLLIVAHRLAPAPTIAPFIYVQLVAVTVIGYLVFGDLPDVWTITGSA
                     IIVASGIYLLRREQTVEAEKS"
     gene            complement(559..1161)
                     /gene="gmk"
                     /locus_tag="VFX46_06200"
     CDS             complement(559..1161)
                     /gene="gmk"
                     /locus_tag="VFX46_06200"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX5600206.1"
                     /translation="MLVLSSPSGAGKTTLARRLLASESGIEMSVSVTTRKPRPGEEEG
                     KDYYFVSESEFRRMRDAGELLEWARVFDNFYGTPRSPVERALAAGRDVLFDVDWQGAQ
                     QLNEKVSQDLVRVFILPPSGEVLEQRLRSRREAPEVIQRRMADAAAEISHWAEYDYVI
                     INSDLETSVAGLRSILAAERLRRERLLGLSEFVREMQKSL"
     gene            complement(1199..1744)
                     /locus_tag="VFX46_06205"
     CDS             complement(1199..1744)
                     /locus_tag="VFX46_06205"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEX5600207.1"
                     /translation="MLELIEVPESEEEIEERTEAMLASLEAALRGVEAARASEGARLR
                     EILLSLLAEIERIVETIAASPARTPEAIQARLREQVARLMDASASFDEARLHQEAVLL
                     ATRADIEEELQRLRVHVAAARELLDATEPVGRRLDFLSQEFNREANTLCSKANDGDIT
                     RMGLQLKALIEQMREQIQNIE"
     gene            complement(1772..2089)
                     /locus_tag="VFX46_06210"
     CDS             complement(1772..2089)
                     /locus_tag="VFX46_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX5600208.1"
                     /translation="MTLKSMTGFARADGAAGHTRWHWEVRSVNGRGLDLRLKLAPGYE
                     ALEPRVREAAAKRLRRGNVAVTLSVKREEGAAAQIRLNPDLLEQVLAAAERVREAVGG
                     ASV"
     gene            complement(2220..>3194)
                     /gene="mltG"
                     /locus_tag="VFX46_06215"
     CDS             complement(2220..>3194)
                     /gene="mltG"
                     /locus_tag="VFX46_06215"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5600209.1"
                     /translation="ISSEHIFVAYYLARQVSGWFGGEPLNLKAGEYELKPSISMREVA
                     NLLSEGKVALLRLTIPEGLTSYQIVERIKADQSLTGDILAVPEEGTLLPDTYFVPRGT
                     TRQEVIDLMQTQRDKFLAAAWEARQPDLPFETVEEAVILASIVQREAGRNDKFEDIAS
                     VFINRLRRGMPLQSDPTILYGLEGGQVNWGRPIYRSEIQRRTSHNTYQISGLPPTPIC
                     NPGREAIQAVLNPSQTNYLYFVASGSGSSVFSQTLEEHNVAVAKWRKIEREIRAKQAE
                     AAKAKTLAASDAEVPANQPVEAAPAAAMAEGGDADDARPPLPIRKPKR"
BASE COUNT          539 a         1064 c          931 g          660 t
ORIGIN      
        1 ctgctggctg gcccctttct cggcgaatgg gtgggctggc ggcggcttat tgcaatcctg
       61 acagggttca ttggcatttt ggtcaccatc cggcccggct tcgcggcgtt tcaccctgcg
      121 ttcctcttgg cgctcggatg catgctctcc tattccctgt atattcttct cacgcgttat
      181 ctcgccgcct acgacacatc cgaggtcaca ctgctttatt cgaccctcgt tggcacggcc
      241 ttgctggcgc cgtgggccat cggcgattgg gtatggccga cgaacgcctt cacgtggatg
      301 gtgctgatgt cgctcgggtt ttggggcggc ctgggccact atctgctgat tgtcgcccac
      361 cgcttggcgc ccgcaccgac gatcgcaccc ttcatctacg ttcagctcgt ggccgtgacc
      421 gtgatcggct atctcgtctt tggcgatctg cccgacgtgt ggacgatcac aggctcggcc
      481 atcatcgtgg catcgggaat ttacctgttg cgaagggaac aaacggtgga agccgagaaa
      541 tcgtgaagtc gcgccaattc acagcgactt ctgcatctcg cgcacgaact cgctcagacc
      601 gagcagccgc tctcggcgca accgttccgc tgcgaggatc gaccttagcc cagcgacgct
      661 ggtttccaga tcggaattta tgatgacgta gtcgtactcg gcccagtggc tgatctcggc
      721 agcggcatca gccatccggc gctggatcac ctcgggcgct tcgcggcggg acctcagacg
      781 ctgctccagc acctcaccag agggtggcag gatgaacacc cgcacaaggt cttgtgaaac
      841 cttctcattg agctgctgcg ccccttgcca gtcgacatcg aagagcacgt cccgtccggc
      901 ggccagcgcc cgctcgaccg gggatcgcgg cgtgccgtag aaattgtcaa agacgcgcgc
      961 ccattcgagc agctcccccg catcccgcat gcgacgaaac tcggactcgc tgacgaaata
     1021 gtaatccttc ccctcctcct caccgggacg cggcttgcga gtcgtgaccg atacggacat
     1081 ttcgatcccg ctttcggagg cgagcagtcg gcgggcgagc gtggtcttgc cggcgcccga
     1141 cggcgacgac aagaccagca tgatgccacg gcgctcgatg gtcggcgaat gcacggcgtc
     1201 actcgatgtt ctgaatttgc tcacgcatct gctcgatcaa cgccttgagc tgcaggccca
     1261 tccgcgtgat gtccccgtca ttggccttcg agcacagcgt gttcgcctca cgattgaact
     1321 cctgcgacag aaaatcgagc ctccgcccta cgggttcggt cgcgtcgagc aactcacgag
     1381 cggccgcgac atgaacgcgg agacgttgca gctcttcctc gatgtcggct cgggtcgcaa
     1441 gcaagacagc ctcctgatgc agccgggcct cgtcgaacga tgcggaggcg tccatcagtc
     1501 tcgccacttg ctcacgcagt ctggcctgaa tcgcctccgg tgttcgagcg ggcgaggccg
     1561 caatggtctc aacaatgcgt tcgatctcgg cgagaaggct caacagtatt tcgcgcaacc
     1621 gcgctccttc cgatgcgcgc gccgcctcca cccctctcag cgcagcctcc aggctggcaa
     1681 gcatggcctc ggtgcgctct tcaatctcct cctcgctctc ggggacctcg atcagctcga
     1741 gcacgccctt gactgcgagg atactgtcga cctacacgct cgcgccgccc accgcctccc
     1801 gcacacgctc cgctgctgcc aacacttgct ccaacaggtc cgggttcagc ctgatctgcg
     1861 cagcagcgcc ttcctcgcgc ttaaccgaca gcgtgacggc aacgttgccg cgtctcagcc
     1921 gtttcgccgc cgcttcgcga acgcgtggtt caagcgcttc ataacctggc gcgagcttaa
     1981 ggcgcaggtc gagcccgcgg ccattgacac tgcgcacctc ccagtgccag cgcgtatggc
     2041 cagccgcgcc gtccgcgcgt gcgaagccgg tcatgctctt gagcgtcatc gggtaccaca
     2101 agccgcgggc gccagctggc tccgcgtgaa tcgtcaattg atgcgcgggg acactaaccg
     2161 cttggccact cgccgacaag caaacacggg gccgcgcccc gtctggttct ggtcttacct
     2221 taccgcttgg gctttctgat cggaagcggt ggtctggcgt catccgcgtc gccgccctct
     2281 gccattgcgg cagcgggagc agcttctacc ggttgattgg ccggaacttc tgcgtcgctg
     2341 gccgccaatg tcttcgcctt cgcggcctcc gcctgtttgg cgcgaatctc ccgctcgatt
     2401 ttccgccact tcgcgacggc gacgttgtgc tcctccaacg tctgcgaaaa cacgctggaa
     2461 ccggaaccgc tcgcgacgaa gtagagatag ttggtctggg aagggttgag gacagcctgg
     2521 atcgcctcgc gtcccgggtt gcagataggc gtcggcggga gcccgctgat ctggtatgtg
     2581 ttgtgactgg tcctccgctg gatctcgctt cggtaaatcg ggcgtcccca gttcacctgt
     2641 ccgccctcga gcccgtagag aatggtgggg tcagactgta gaggcatccc gcggcgcagc
     2701 cgattgatga acaccgacgc gatgtcctca aacttgtcgt tgcggcccgc ttccctctgg
     2761 acgatggatg cgaggatcac cgcttcctcg acagtctcaa acggcagatc gggctgacgt
     2821 gcctcccaag ccgccgccag gaacttgtcc ctctgcgtct gcatgaggtc gatcacttcc
     2881 tggcgggtcg ttccgcgagg cacgaaataa gtatcaggta acagcgtgcc ttcctccgga
     2941 accgccagga tatcgccagt caaactctga tctgccttga tccgctcgac gatctgatag
     3001 ctggtcagcc cctcgggaat tgtcagccgt aggagagcga ctttgccttc gctgaggagg
     3061 ttcgccacct cacgcatact gatggatggt ttcagctcgt actcgccggc cttgagattg
     3121 agaggctccc cgccaaacca gccggatact tggcgtgcca agtagtacgc aacgaagatg
     3181 tgctcgctcg aaat
//
LOCUS       DASUMG010000379         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_692175, whole genome shotgun sequence.
ACCESSION   DASUMG010000379 DASUMG010000000
VERSION     DASUMG010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692175"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="VFX46_06220"
     CDS             complement(<1..2379)
                     /locus_tag="VFX46_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5600210.1"
                     /translation="MSPIEVCIRRPVFATVLSLVLVLLGLVSYTRLTVREYPNIDEPT
                     VSVTTTYPGASPHIVETQVTQVLEGSLAGIAGIDVLESTSRSETSRITIRFRQDVNPD
                     EAANDVRDRVSRVRGRLPDEIQEPVIRKVEADAQAIMYIVFTSDQMSALEITDYVDRY
                     VVDRLKNVTGVADVMIYGERRYAMRIWIDRERLAAYSLTIQDIENALRSQNVEIPSGR
                     IESQEREFSVLSRTGLSTPEEFREIIVKRANGYQVKLGDVAQVELGAEDERRDSRYNG
                     QPAIVVGIIKQAVANPLDVSRGIRALLPDLNESLPSGLKADIGNDDAVFINESIKAVF
                     STILEAIVLVVIVILFFLRSIRASIIPIVTIPISLIATFTLLLAFGFSINTLTLLAMV
                     LAIGLVVDDAIVVLENVSRHVENGMRPFQAAITGTREIAFAVVAMTLTLTAVYAPIAF
                     ATGRTGRLFLEFALALAGAVLVSGFVALTLTPMLCSKLLRHDESPGWIFTAIERGLRS
                     LEGGYRRALRAALSVRWLIVILGLATAGVAAVLFVNLKSELAPVEDRGVIMIRGNGPE
                     GSTLSYMARYSAQADAILAELPEVRSRLVINGLWQVTNFMAIGRLHDWSERDRKQQDL
                     VAEINPKIRNIPGVLAFAQNPASLGQRGSSRPIEFVVQTSGTYEQLQEYADRMLERIS
                     EYPGLEGVDTDLKLNSPEVRIDVNRAKIADLGIDVATVGRTLETLLGGRQVTRFEQNG
                     EQYDVIVQLAGADRSTPEMLSTIFLRGANGEMVQLSNIVTVSETVAPKELKRF"
     gene            complement(2379..3473)
                     /locus_tag="VFX46_06225"
     CDS             complement(2379..3473)
                     /locus_tag="VFX46_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5600211.1"
                     /translation="MRRLLPLLLALVVAGGLAIALLPDGRLALRAEKSGKETPRQSQA
                     VAVEVAPAIAARYQEDLRSVGSLMSDESVQIASEIAGRIQSFEFAEGQAVKAGDVLVK
                     LDDSLARAELADAKARFELARANRERAQLLRKQGHMSQQAHDEAAANYQIAEAALELA
                     SVKLSKHVIKAPFDGIVGLRNASTGAYVNIGTPIVNLEKIDFLKIDFKLPEIALTQIS
                     TGQDVMVEVDAVPGRTFKAQVYAISPMVDVNGRALHVRARMENPDLLLRPGLFARITV
                     KGRETREVVAVPESAIVPRGGESFVYVIQGGKAVESKVTLGKRENGTVQVLSGLSAGS
                     TVVVAGQLKLRDGATVEVVQTVSGPELEKS"
     gene            complement(3803..>4055)
                     /locus_tag="VFX46_06230"
     CDS             complement(3803..>4055)
                     /locus_tag="VFX46_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX5600212.1"
                     /translation="TDHRIGRIMNHNLAEYHVPVHADVGNIDVIFVDEPDPHVSPLGV
                     KGLGELGIVGAAAAIANGIFHATGKRIRDLPITIDKIMA"
BASE COUNT          790 a         1238 c         1232 g          795 t
ORIGIN      
        1 gaaacgcttc agctccttgg gagccaccgt ttcgctgact gtcacgatat tggagagctg
       61 caccatttct ccgttggctc cccttaggaa gatggtcgac agcatttcgg gggtgctgcg
      121 atcggcacca gcaagctgaa cgatgacgtc atactgctca ccgttctgct cgaaccgcgt
      181 aacctgacgc ccgcctagaa gggtttcgag cgtccggccc actgtcgcga cgtcgatccc
      241 taagtcggcg atcttggccc ggttcacgtc gattcgaacc tcgggggagt tgagcttcaa
      301 atcggtgtcg acgccctcga gtccgggata ttcactgatc cgctcgagca tccggtcggc
      361 atactcttgc agttgctcat aggtgccgga tgtctgaacg acgaactcga tcggccgcga
      421 gctgccacgt tggccgagag aagcagggtt ttgcgcgaag gccagaacgc ccggaatgtt
      481 ccggatcttg gggttgatct ccgcgacgag atcctgctgc ttgcggtcac gctccgacca
      541 atcgtggaga cggccaatgg ccatgaagtt ggtgacctgc caaagaccat tgatgacgag
      601 gcgcgagcgg acctccggca attcggccag gatggcatcg gcctgggcgc tgtagcgggc
      661 catgtatgac agtgtcgagc cttccggtcc gttgccgcgg atcattatga caccgcggtc
      721 ctcaacagga gccagctccg atttcaaatt gacgaacagg acagcagcaa cacccgccgt
      781 cgccaaccct aggatgacga tcagccatcg cacagagagg gcagctcgaa gggcgcgccg
      841 gtagcccccc tcgagactgc gcagcccacg ctcgattgcg gtgaagatcc atccaggcga
      901 ctcgtcgtgc cgcagcagct tggagcagag catcggcgtc agagtcagcg caacgaagcc
      961 agaaacgagt accgcccctg caagcgccag cgcgaattcc aggaaaagcc ggccggtgcg
     1021 gcccgtggcg aacgcgatgg gggcgtagac ggccgtcagc gtcagcgtca tcgccacgac
     1081 tgcgaatgcg atctcgcgcg tgcccgtaat ggccgcttga aagggccgca tgccgttctc
     1141 gacgtggcgg gaaacgttct caagaacgac gatggcgtca tccacgacga ggccgatcgc
     1201 caagaccatc gcgagcagcg tcagcgtgtt gatgctgaag ccgaatgcca ggagcagcgt
     1261 gaaggtcgcg atcagcgaga ttggaatggt gacgatgggg atgattgatg ctcgtatcga
     1321 cctcaggaag aagaggataa cgatcaccac gagcacgatg gcctcgagaa tcgtgctgaa
     1381 caccgctttg atcgattcgt tgatgaagac ggcatcatcg ttgccgatgt ctgccttgag
     1441 gccgctaggc agagactcat tcaggtcggg caggagcgcg cgaatgcctc ttgaaacatc
     1501 gagcgggttc gcgaccgctt gcttgatgat gccgacgacg atcgcgggct ggccgttgta
     1561 cctgctgtca cggcgctcat cctcggcacc gagctccacc tgcgcaacgt cgccaagctt
     1621 gacctgatag ccatttgctc gcttgacgat gatctcccgg aattcctcag gcgtgctcag
     1681 gcccgtgcgg gaaagaacgc tgaactcccg ttcctggctt tcgatgcggc cagagggaat
     1741 ttcgacgttc tggctacgca gtgcgttctc gatatcctgg atcgtgaggg agtacgcagc
     1801 caggcgctca cggtcgatcc aaatccgcat ggcgtagcgg cgctcgccgt agatcatcac
     1861 atccgccacg ccggtcacgt ttttcaggcg atctacaaca tatcgatcga cgtagtcggt
     1921 gatttccagc gctgacatct gatcgctggt gaaaacgatg tacatgatcg cctgagcatc
     1981 cgcctcaacc ttgcggatga ccggctcctg gatctcgtcg ggtagtcgtc cgcgaacgcg
     2041 gctgacccgg tcacgtacat cgttggcggc ttcgtcggga ttgacgtctt gccggaagcg
     2101 gatggtgatg cggctcgtct ccgagcggct cgtggactcg aggacgtcga tgccggcgat
     2161 tccggcaagc gagccttcga gaacctgcgt aacctgcgtc tcgacgatgt gtggcgaggc
     2221 tccaggatac gtcgtggtga ccgaaacggt gggctcgtcg atgttcgggt actcgcggac
     2281 ggtcagtcgc gtgtacgaaa cgagcccgag cagcaccagg acgaggctga ggaccgtcgc
     2341 gaaaaccggg cgccggatac agacctcgat cgggctcatc agctcttctc cagctctggg
     2401 ccgctgacgg tctggacaac ctcgacggtg gcaccatccc ggagcttgag ctggccggcg
     2461 acaacgacag tggatccggc gctgaggcct gacagcactt ggaccgtccc attctcgcgc
     2521 ttacccaagg tcactttgga ttcgaccgcc tttccgccct ggatcacgta gacaaaagat
     2581 tcgccgcctc ggggaacgat cgcgctttca gggaccgcca ccacttccct ggtttctctt
     2641 cccttcacgg tgatccgagc gaagagccct ggccggagca gaagatccgg attttccatg
     2701 cgcgcccgca cgtgcagggc acggccatta acgtccacca tcgggctgat ggcataaacc
     2761 tgtgccttga acgtccggcc gggcaccgca tcgacctcca ccatgacatc ctgcccagtg
     2821 ctgatctgcg tcagagcaat ctccggcagt ttgaagtcga tcttcaggaa gtcgatcttc
     2881 tccaggttta cgatcggcgt gccgatattc acgtaagcgc cggtggaagc attccgcagc
     2941 ccgacgatgc cgtcgaacgg cgccttgata acatgcttgg agagcttgac cgaggccagt
     3001 tcgagagcgg cctctgcgat ttgatagttc gccgcagcct cgtcgtgcgc ctgctgcgac
     3061 atgtggccct gctttctcag cagttgcgcg cgctcacgat tggcacgtgc gagctcgaat
     3121 cgcgcttttg cgtccgccag ttcggcacgg gccagagagt cgtcgagctt gacgagcacg
     3181 tcgccagcct tgacggcctg tccttccgca aactcgaagc tttggatgcg gccagcgatt
     3241 tccgaggcaa tctggacgga ctcgtccgac attaagctgc cgacggagcg caggtcttcc
     3301 tggtacctgg ccgcgattgc gggcgcgacc tccacggcga cagcctggct ctggcgaggc
     3361 gtctccttgc cactcttttc agcgcgcagc gccaagcggc catctggcaa caacgcgatc
     3421 gccagtccgc cggcaacgac tagcgccagt aagagcggca gaagcctacg catcgcgtcg
     3481 tcctcacccc ctggaagcgg tcttgatgtt cttgttgctc gcttggttaa ccgaagtgcc
     3541 tgttctctca gaacggatca gcacctcgga acccgtcgcg acaatataca cccgtcgaag
     3601 accgtaacag aacacaactg cttgttggcg agagtttatc ccgcaattcg gctacctgga
     3661 gcctaatcca atggatgatg cggaaattcg ccaggcccag cctgacccgg ccgtgatgat
     3721 gcgagcgaaa cgggccgcaa tccagcgacc tgtcgtacga acgcaatggt atggcgcggt
     3781 ggtgccgcta ccggcctctt gctcaggcca ttatcttatc gatcgtgatc ggcaagtcgc
     3841 ggatcctctt gccggtggcg tggaatatgc cgttggcgat tgccgcggcc gcgccgacga
     3901 tcccgagctc gcccagcccc tttacgccaa gcggcgagac gtggggatcg ggctcgtcaa
     3961 cgaagatcac atctatgtta ccgacgtcag catgcaccgg gacgtgatac tcggcgagat
     4021 tgtggttcat gatgcggccg atgcggtgat cggta
//
LOCUS       DASUMG010000380         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1013881, whole genome shotgun sequence.
ACCESSION   DASUMG010000380 DASUMG010000000
VERSION     DASUMG010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013881"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VFX46_06235"
     CDS             complement(<1..347)
                     /locus_tag="VFX46_06235"
                     /inference="COORDINATES: protein motif:HMM:NF023916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease family
                     protein"
                     /protein_id="HEX5600213.1"
                     /translation="MLSFAFAVLLVLTLIGFLVGHRRAIACVEGRTYQLHSRPAYHAS
                     FVAAWVGIPATLLLLVWLLVQGPVLDRLIMASLPDEVVAGKGVGELSLILSQIKSVAD
                     GTSFGQVDPAILEA"
     gene            complement(471..1535)
                     /locus_tag="VFX46_06240"
     CDS             complement(471..1535)
                     /locus_tag="VFX46_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5600214.1"
                     /translation="MNRTLVAISAAAAVAGTIGFAGEAEARDHIKIVGSSTVFPYTQA
                     VAEEFTRKNGQKAPVVEATGTGGGMKIFCQGIGEQYPDITGASRAMKKSEWAECEKNG
                     VTDVTEVLIGYDGISIAQSRKAQPMDLTKAQIYLALAAEIPVDGKFSPNPHKKWSDID
                     SSLPNIPILVYGPPPTSGTRDAFVELAMQEGCQELDFVKEQKARLDKAAYEDLVKSQC
                     SRMRQDGPFIEAGENDNLIVQRLEADPTAVGIFGYSFLYENEDKLQAIKVEGVEPSFE
                     TIASGEYGISRPLYLYVKNAHRDVVPGMKEFIAEYVSEAAMGQDGYLSERGMVPLQAD
                     ALEKTRKAALEAERMAVPTN"
     gene            complement(1699..2940)
                     /locus_tag="VFX46_06245"
     CDS             complement(1699..2940)
                     /locus_tag="VFX46_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5600215.1"
                     /translation="MIVLLWLTGGMAAWAAALALVALAGGLVLAPQQPISAPPASAPQ
                     VEQRPTESPSVTIASRDASWRAMVSAMPDPALVLDDAGIILHHNAAAADLFPRIRAGQ
                     HLSSVSRNPDLLSAVEKASDTADVITVELLDRVPVQRRVSAILTPLSFEQRVDAGPAY
                     LVTFRDITDQEKLAQMRADFIAHASHELRTPLASLRGFVETLQGPARDDPEARDRFLG
                     IMAAQAERMTRLIDDLLSLSRIEMHVHVPPRGTVELNEVVNFVAQSLEPVAQSSGISI
                     EVETLPTSAFVRGDYDELVQVFQNLIQNAIRYGREGGFVRVRMTREPHRRGEGQRISV
                     AVEDNGEGIAPEHLPRLTERFYRVSVSASRQKGGTGLGLAIVKHIVTRHRGELKIAST
                     LGKGSTFTVLLDELSESTAAP"
     gene            complement(3127..3990)
                     /locus_tag="VFX46_06250"
     CDS             complement(3127..3990)
                     /locus_tag="VFX46_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600216.1"
                     /translation="MTAHVAEAAEARGRVVLQLRTACPNEVAIEAALRVAQAFQSGIE
                     TLFVEDLQIFELANFPFAREISLTGRRRRALSPKEIEQEFRLAFASLQRRIEAMARAA
                     EVPIRQRVVRDDPVAALAATCAECGPWNVIALAEPFNAMSGESLQRLFESVTDATGLV
                     VVGPKARRTTGPVILAVEDIERLPSMVRAGDRLAAVTGGTLVVLPIADDDERVRWMEA
                     QARLLLKDRIDVQVVAAGSARGAAEVVAETLRRLRGGFVISEFGGRVVPADRDLKALA
                     SALECPILLVR"
     gene            complement(3987..>4578)
                     /locus_tag="VFX46_06255"
     CDS             complement(3987..>4578)
                     /locus_tag="VFX46_06255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX5600217.1"
                     /translation="LHSKGVMILWGYLAGRYAEDVPLALAATLVFEQSYGGVDGDSAS
                     STELYALLSALSEVPIKQGIAVTGSVNQRGEVQAIGGVNEKIEGFFDICKARGLTGEQ
                     GVLIPKSNVQHLMLRQDVVEAVREGKFSIWAVSHVDEGMEILTGVKAGERGEDGQFPP
                     GTINRLVEDKLKLFAERARAFGRSKSDSQGNGKITS"
BASE COUNT          869 a         1479 c         1380 g          850 t
ORIGIN      
        1 gcttcgagaa tggccgggtc cacctggccg aaggacgtgc cgtcagcgac ggacttgatc
       61 tggctcagga tgaggctgag ctcgccaacg cctttgccgg caaccacctc atccggcaac
      121 gacgccatga tgagccggtc aagaaccggc ccctggacca gcagccagac caacagtagc
      181 agggtcgccg gaatgcccac ccacgccgcg acgaagctgg cgtgatatgc cggccgcgaa
      241 tgcagttggt aggtgcggcc ctcaacacac gcgattgccc gtctgtggcc gaccagaaat
      301 ccgatcagcg tcaggacgag cagaactgcg aaggcgaagc tcagcatgtc gagttcacgt
      361 ggcgagcctg cggaattgcg cgagccggtt tgactgtccg ttctgcgggt gcaccacaag
      421 cgcgggcggc tgcgccacgt caatggcgca gccggcgttt tcaacggcca tcagttggtc
      481 gggactgcca ttctttctgc ttccaacgcc gccttgcggg tcttttccaa ggcatccgcc
      541 tgcagcggca ccatcccacg ctccgagagg tagccgtcct ggcccatcgc ggcctcgctg
      601 acgtactcgg ctatgaactc tttcattccg ggaaccacgt cgcgatgggc gttcttgacg
      661 tacaagtaca ggggacgcga gatgccatac tcacccgagg cgattgtctc gaacgacggt
      721 tcaactcctt ccaccttgat ggcctggagc ttgtcctcgt tctcgtagag gaacgagtag
      781 ccgaagatgc ccacggctgt gggatcggcc tcgaggcgct gcacgataag gttatcgttc
      841 tcgccagcct caatgaacgg accgtcctgc cgcatgcggc tgcactggga tttcaccaga
      901 tcttcataag cggccttgtc gagcctggcc ttctgctcct tcacgaagtc gagttcctgg
      961 cagccctcct gcatggcgag ctccacgaac gcatcgcgcg tacctgatgt tgggggtgga
     1021 ccatagacga gaatgggaat gttaggcagc gacgaatcga tgtcgctcca cttcttgtgc
     1081 gggtttggac tgaatttgcc atccacagga atttcggctg caagggccag atagatctga
     1141 gccttcgtca ggtccatcgg ctgagccttg cgggactgcg cgatggatat tccgtcgtac
     1201 ccaatcagca cctctgtgac gtccgtgacg ccattcttct cgcactcggc ccactcggac
     1261 ttcttcatcg ctcgagaggc tccggtgatg tccgggtact gctcaccgat gccctggcag
     1321 aagatcttca tgccgccgcc ggtgcccgtc gcctcgacaa ccggtgcctt ttgaccgttc
     1381 ttacgcgtga actcttctgc gacggcttgg gtgtaaggaa agaccgtcga cgagccaacg
     1441 atcttgatgt ggtcgcgagc ctccgcctca ccggcgaagc caattgtgcc tgcgacggct
     1501 gctgccgccg agatggccac aagggttctg ttcacaggtg cactccctgt ctgggtttgc
     1561 agcgggaagc ctctcacaca tcgcttccca ggaaacgacg ccccattgcg tcgcaggctt
     1621 tctgatagag caaacacgct atgctttcgt gacagagtaa gcctttgaat tgaaaaggct
     1681 ttatcgattc ttggcgactt agggggcggc tgtagactca gacagctcgt cgagcagcac
     1741 ggtgaatgtc gagcccttcc ccagcgtcga agcgattttg agctcgccgc ggtggcgggt
     1801 gacgatgtgc ttcacgatgg cgaggccgag gcccgttccg cccttctgcc ggctggccga
     1861 aacgctcacg cggtagaacc gctctgtgag gcggggcagg tgctcaggcg caatgccctc
     1921 tccattgtcc tcgacggcca cggagatccg ctggccctcg ccgcgccggt ggggctcgcg
     1981 ggtcatacgg actctcacga agcctccttc gcgaccgtat ctgatggcgt tctgaatgag
     2041 gttctggaag acctgcacca gctcgtcgta gtcccctcgc acgaaagccg aagtcggcaa
     2101 ggtttcgacc tcgatggaaa tgccagacga ttgcgcaact ggctcaaggg attgcgcaac
     2161 gaagttgaca acctcgttca gctctaccgt ccctcgtgga gggacatgca cgtgcatttc
     2221 aatgcggctg agagaaagca ggtcgtctat gagccgggtc atgcgctctg cctgagcggc
     2281 catgatgccg aggaaccgat cgcgggcttc cggatcgtcg cgcgccgggc cttgcaacgt
     2341 ctcgacgaag ccgcgcagcg aggccagcgg tgtgcgcagc tcgtggctcg catgggcaat
     2401 gaagtcggcc cgcatttggg cgagcttctc ctggtcggtg atgtcgcgga acgtgacgag
     2461 ataagctggc ccggcatcga cacgctgctc gaaagacagg ggcgtcagaa tcgccgagac
     2521 gcggcgctgc accggcacgc gatccaggag ctcgacggtg atgacatccg ccgtatcgct
     2581 ggccttctcg actgccgaaa gcagatcggg attgcggctc acgctggaaa gatgctgacc
     2641 cgcacgaatg cgggggaaaa ggtccgccgc cgccgcgttg tggtggagaa tgatgcccgc
     2701 gtcgtcgagc accaaggccg gatccggcat agcactgacc atcgcacgcc agctcgcatc
     2761 gcggctcgcg atggttactg acggagactc ggtaggacgt tgctcaactt ggggagcgga
     2821 agccggcggc gcggaaatcg gctgctgcgg tgcaagcacg agccctccgg ctaaggccac
     2881 cagggccaac gcagccgccc acgccgccat gcctcccgtg agccatagga ggacgatcat
     2941 cgcagtgccg gcagcgagcg gtgctagccg cgccgccaat cgccgggcga gcttttcacc
     3001 tagccaaagc agatgctggc aagtggaata tcctgactga agccgctgat ggggggctat
     3061 tgctctcacc ctcactgaat gcgtgccgac agccaggaat taaggtgctc gcgcaagccg
     3121 ggcaagtcag cgcacgagga ggatggggca ctccaatgcg gaagcaagcg ctttcagatc
     3181 gcggtctgct ggcaccaccc gaccgccaaa ctcgctgatc acgaatccgc ctctcagacg
     3241 ccgcagcgtc tctgcaacca cctcggcagc accgcgtgca gagccggccg cgaccacctg
     3301 cacatcaatg cggtccttga ggagcaggcg ggcttgagcc tccatccagc gcacccgctc
     3361 gtcgtcgtcc gcgattggca agacaacgag cgtgccgccg gtgaccgccg ccaggcgatc
     3421 gccagcccga accatgctcg gcaatcgttc gatgtcctcg acagcaagga tcaccggccc
     3481 tgtcgtacgc cgcgccttgg gtcccacgac caccaagccc gtggcatccg tgacggactc
     3541 gaagaggcgc tgcagggact caccgctcat cgcgttgaac ggctcggcca aagcaatcac
     3601 gttccacggc ccgcactcgg cacacgtggc tgccagcgcg gcgacgggat cgtcgcgcac
     3661 gacccgctga cggatcggca cctccgcggc cctggccatc gcctcgatgc ggcgctgaag
     3721 cgacgcaaac gccagccgga actcctgctc gatctccttt ggcgacaggg cacgcctcct
     3781 gcgccccgtc agcgagatct cccgggcgaa tggaaagttt gcgagctcga agatctgcaa
     3841 atcctcgacg aaaagcgtct cgatccctga ctggaacgct tgcgcgacgc gcaacgcggc
     3901 ctcgatcgcc acctcattgg gacatgccgt gcgcagctgc agtacgacgc gcccacgcgc
     3961 ttctgcagcc tccgcgacat gcgcagtcat gacgtaatct ttccgttacc ctgtgaatcg
     4021 cttttcgagc ggccgaacgc tctggcgcgc tcggcaaaaa gcttcagctt gtcctcgacc
     4081 aatctattga tcgtacctgg cgggaattgt ccatcctcac cccgctcgcc ggccttgacg
     4141 cccgtcaaga tctccatgcc ctcgtcgaca tggctcacgg cccagatgga gaacttgccc
     4201 tcgcgcacgg cctcgacgac gtcttgccgt agcatgaggt gctgtacgtt ggattttggg
     4261 atgagaacgc cctgttcccc ggtcaggcca cgcgccttgc agatgtcgaa gaagccttcg
     4321 atcttctcgt tgaccccgcc gatcgcctgc acttcacccc tctggttgac cgagcctgta
     4381 acggcaatgc cctgtttgat cggtacctcg gagagtgcag aaagcagcgc gtaaagctct
     4441 gtggaggagg cactgtcgcc gtcgacaccc ccataggact gctcaaaaac gagggttgcc
     4501 gccagcgcca atggtacatc ctcggcataa cggccggcaa gatagcccca caggatcatc
     4561 acccctttgg agtgaagc
//
LOCUS       DASUMG010000381         3168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_933419, whole genome shotgun sequence.
ACCESSION   DASUMG010000381 DASUMG010000000
VERSION     DASUMG010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933419"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VFX46_06260"
     CDS             <1..430
                     /locus_tag="VFX46_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600218.1"
                     /translation="GFGLGAQILAIAAEGAAEEAPLELQVGEARRTKEGALGGFLPET
                     FPNVVYMRDRPVPPDYADVLAVDEAGLPALFQIGKNAFGFSGHPGVRRAMIEDLIMEF
                     EEAPEDTGPGLDRLAGLATAIEDALVPIMTGLIQATGWMR"
     gene            complement(439..1383)
                     /locus_tag="VFX46_06265"
     CDS             complement(439..1383)
                     /locus_tag="VFX46_06265"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5600219.1"
                     /translation="MIHNGDAAAGLPRQRKVCAMVDTAVIRTQPRVLRALAWMGVAVA
                     SFSSAAIAAREAARGATTLEMMFYRCLISLVIVLAIVLMGPGLKAMRTTRLPLHSLRG
                     LLHFIGQYSWLGALTMIPLAQLFALEFTAPIWVALLAPLLLGERMTTTRLAAAVVGFI
                     GAIVVAQPGTVEFNAGVGLGLLAAIGFALTMLTTKVLSHGESPLRILFYMFLIQTVLS
                     LLLLIGGIRLLDPATIGWISALAITGLAAQYALVRAFALADAIMVAPMDFLRLPLIAV
                     VGTIVYAEPFDPIVLFGGLVVIAGNLLNLWGERRAARS"
     gene            1530..1907
                     /locus_tag="VFX46_06270"
     CDS             1530..1907
                     /locus_tag="VFX46_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX5600220.1"
                     /translation="MAKKLVIVMANTDPRNGEELGAPIFQASVAAAMDYDVEVICTAT
                     AGRLMKKGVAEKLVVKPGSPKTVYDFIKEAHEAGVKFYCCSPNLDLFDMSKDDLIPEC
                     SGIVGGAYLIEEVMEGDTKVLSY"
     gene            1981..2739
                     /locus_tag="VFX46_06275"
     CDS             1981..2739
                     /locus_tag="VFX46_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX5600221.1"
                     /translation="MTETAAATRFQDYIGDPVSEKPPVPREKLEEIFNDLQSDLRFDH
                     ELNGCLNCGICTATCPAAHYYDFSPREIVQLLWTENLEGIYDAMQEKIWACAQCYTCA
                     ARCPFKNSPGGLIMLMREVAIKHQMQSAKDVLRPFSRVMLKLISTGNQLSPDMINPQH
                     FPDWGPNISKIDAPLKVLRKAIPMATLNTTETAWEVNLKTSIELYTIWEMTGVLDQLE
                     GLDENLFDVITDFMDEKREDWADFLEEQEEAEDD"
     gene            2790..>3168
                     /locus_tag="VFX46_06280"
     CDS             2790..>3168
                     /locus_tag="VFX46_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase subunit B"
                     /protein_id="HEX5600222.1"
                     /translation="MSMDGTGRPTQPGERFTGHGPEWRDSNLSPREAAIATAWVEQKI
                     DRRSMLTNKDRVEDVRDAMWQLEKDGEIVVHRITDAHEPITVKTLYGWEKKIPTNRLW
                     HHKSCGQCGNIPGYPVSLLWLQNK"
BASE COUNT          704 a          922 c          970 g          572 t
ORIGIN      
        1 cgggttcggg ctcggagcac agatcctggc catagccgcg gaaggtgccg cggaggaggc
       61 gccgctcgag ttgcaagtcg gcgaagctcg tcgcacgaag gagggcgcgc tcggcggatt
      121 cctgccggag acgtttccca atgtggttta catgcgtgac cgcccggtgc cgcctgatta
      181 tgccgatgtc ctggcggtgg atgaggcggg tctgccagcg ctgtttcaaa tcggcaagaa
      241 tgcattcggg ttcagcggcc accccggcgt gcggcgggcc atgatcgagg atctcatcat
      301 ggagttcgag gaggcaccgg aggacacggg tcccggcctc gaccgtttgg ctgggctcgc
      361 aactgcgatt gaggacgccc tggttccgat catgacgggg ctgatccagg ccacgggatg
      421 gatgcgctga cttgtggttc agcttcgagc ggcacgccgc tcgccccaca gattgagcaa
      481 gttccccgcg atcaccacga ggccaccgaa cagtacgatg ggatcgaacg gctcggcgta
      541 aacgatcgtg cccacgaccg cgatcaatgg cagccgcaag aagtccatgg gtgcgaccat
      601 gatcgcatcg gccagagcaa aggcccgcac cagcgcatac tgggcagcaa gccccgtaat
      661 tgccaaggca ctgatccaac ctatcgttgc cggatcgagt aatctgatcc cgccaataag
      721 aaggagcagc gatagcaccg tctggatcag aaacatgtaa aacaggatcc ggagcggact
      781 ttcgccgtgg ctcagcactt tcgtggtcag catggtcaaa gcaaagccga ttgcagccaa
      841 aagccccaag ccgacacctg cgttaaattc cacggtgcca ggctgagcga cgacgatcgc
      901 cccaatgaag ccaaccacgg ctgcagcaag gcgggtcgtc gtcatccgtt caccgagcag
      961 caaaggagcc agcaaagcca cccagattgg cgcagtgaat tcgagcgcaa agagctgggc
     1021 cagcgggatc atggtcaagg cgcccagcca gctatactgc ccaatgaagt gaagcaggcc
     1081 gcgcagggaa tgcagcggca gccgcgtcgt gcgcattgcc ttcaatcccg gccccataag
     1141 gacaatcgcc agcacgatca ccagcgagat gaggcagcgg tagaacatca tttcgagcgt
     1201 ggtcgctccg cgcgcagcct cccgggcggc gattgcggcg ctgctgaacg aggcaaccgc
     1261 aactcccatc cacgccagcg cgcgtaaaac ccgcggctga gtccggatca ccgccgtgtc
     1321 caccatggcg cagacctttc tctgccgggg aaggccagcg gcagcatccc cgttgtgtat
     1381 cattacattc gcaagtacga atattcgctt gccggcagtc ggcggaccga ttaggcttgc
     1441 tgtcagtcgc gcttcacgcc cttttggcgt tagcatagcg gtcatcaaag cccaagacac
     1501 gaacaattgg gagggctccg aggaaacgca tggccaagaa gctcgtgatc gtcatggcca
     1561 acaccgaccc gcgaaacggg gaggagcttg gcgcgcccat tttccaagcc tccgtggctg
     1621 cggcgatgga ctacgacgtc gaggtgatct gcaccgcaac agccggccgg ttgatgaaaa
     1681 agggcgtcgc cgaaaagctt gtcgtgaagc ccggtagccc caagactgtt tacgacttca
     1741 tcaaggaggc ccacgaggcg ggggtgaagt tttactgctg ctcgcccaac ctcgaccttt
     1801 tcgatatgag caaagatgac ctcattccgg agtgctccgg aattgtgggt ggagcgtatc
     1861 tcatcgagga ggtcatggaa ggcgatacga aggtattgtc ctattgagag aggtgaacgg
     1921 ccaattctct tttgacgttc ggctccttcg ctactctcta ttcggtattc gctagaggca
     1981 atgaccgaaa cagctgcagc aactcgcttt caggattaca tcggcgatcc ggtctcggag
     2041 aagccgcctg tgccgcgcga gaagctggaa gagatcttca atgacctcca gtcggatctg
     2101 aggttcgacc acgagctgaa cggctgcctt aattgcggca tctgcacggc aacctgccca
     2161 gcggcgcact attacgattt ttcgccgcgc gagatcgtcc agcttctttg gacggaaaat
     2221 ctcgaaggca tctacgatgc catgcaggag aaaatatggg cttgtgccca gtgctacacc
     2281 tgcgcggccc gctgtccgtt caagaactcg cccggcgggc tcatcatgtt gatgcgcgag
     2341 gtagcgatca agcatcagat gcagtcggcc aaggacgtgc ttcgtccgtt cagccgtgtg
     2401 atgctgaagc tcatctcgac cggcaaccag ctgtcgccgg acatgatcaa tcctcagcat
     2461 tttccagact ggggccccaa catctcgaaa atcgatgcgc cgctgaaggt gttgcgcaaa
     2521 gccattccga tggcgacgct caacacgacg gagacggcgt gggaggtcaa cctgaagacc
     2581 tcgatcgagc tctacacgat ctgggagatg actggcgttc tcgatcagct cgagggcttg
     2641 gacgaaaacc tgttcgacgt gattaccgac tttatggatg agaagcgtga ggactgggcc
     2701 gacttcctgg aggagcagga ggaggccgag gacgactgat cggaatcgtg tccgccctag
     2761 gggccgaccg cgcaaattgg aggtccgata tgtctatgga tgggactggc cgaccgacac
     2821 aacccggcga gcgtttcact ggccacgggc cggaatggcg ggattccaat ctaagcccgc
     2881 gcgaggcggc tattgcgacc gcctgggtcg agcagaagat cgacaggcgg tccatgctga
     2941 ccaacaagga tcgcgtcgag gacgttcgcg atgcaatgtg gcagctggag aaggacggcg
     3001 agatcgttgt tcaccgcatc acggacgcgc acgagccgat caccgtcaag acgctctacg
     3061 gctgggaaaa gaaaattcca acaaaccggc tttggcacca taagagctgc gggcaatgcg
     3121 ggaatatccc gggctatccc gtttcattgc tctggctcca gaacaagc
//
LOCUS       DASUMG010000382         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_291103, whole genome shotgun sequence.
ACCESSION   DASUMG010000382 DASUMG010000000
VERSION     DASUMG010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291103"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(864..940)
                     /locus_tag="VFX46_06285"
     tRNA            complement(864..940)
                     /locus_tag="VFX46_06285"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(904..906),aa:Met,seq:cat)
     gene            complement(1101..1460)
                     /locus_tag="VFX46_06290"
     CDS             complement(1101..1460)
                     /locus_tag="VFX46_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5600223.1"
                     /translation="MDFLATAATKTAAWWTSTPTAEKVWLAVGFVAQLMFSMRFIVQW
                     IASERARRSIVPETFWYFSLAGGALLFAYAIYRLDPVFMLGQGMGLFIYARNLHFILR
                     SKRLPAAPDGEVAGRQS"
     gene            complement(1501..2310)
                     /locus_tag="VFX46_06295"
     CDS             complement(1501..2310)
                     /locus_tag="VFX46_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX5600224.1"
                     /translation="MRISVVIPALNEAGNIGRLVEETYATVPADYLAEVIVVDDASED
                     ATGEEVKALIASGRCPGLRYLRHGTRAGQSAAMRTGILAASSPIIATMDGDGQNDPRD
                     IPRLLARLAPPGTSGPALVGGIRTDRKAEGAKRFASKAANWIRDAVLRDSCPDTGCGI
                     KVYWREVFLRLPFFTSMHRYLPALFLTYGHEVAYVPVNDRPRQAGQSKYNNLSRALIG
                     LYDLIGVSWLRRRTRVPEIVEDVPEGRHISDLGRRLKLRSDGLVRKTGDKS"
     gene            2416..3975
                     /locus_tag="VFX46_06300"
     CDS             2416..3975
                     /locus_tag="VFX46_06300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX5600225.1"
                     /translation="MGNGGLHGLLADVTGERQTMTVQPGLEGDRVAGEAWFPALVAVL
                     GGLLVVRMVALAFNATDLFFDEAQYWSWSLEPAFGYYSKPPLIAWVIRVATEACGHSE
                     FCIRLPSPIIHSATALVIYAVAARLYDRRVGFWSAVVFATLPGISLSAGIISTDVPLL
                     FFWAVALYALVRLLDSDRWWPAVLLGVALGLGLNAKYAMVYFLLSAAAYMVAVPEKRR
                     LLHDARAYAALGIGLLLIAPNLAWNAANSFATFAHTADNANWGGTLFHPEKALEFFGA
                     QFGVFGPILFGSLLVITWRAWQDGLPEPDRLLLAFALPVIVLVTIQAFLSRAHANWAA
                     VSYVSATVLVTATMLREGAWRWFKSSLALHAAILVGIGLATAAAGSFSLPVGGDPFAR
                     TLGWRELAQETATVLAEARQSGRPFGAVIADDRAITAELLYYMRDEPTSVFAWREPGV
                     RPKDHYELTRAYQRGSPEPVLLVSFRRGAERVSSRFAVVELIVEREVPAGAGPGRKVA
                     FYRLEGFAGVE"
BASE COUNT          741 a         1205 c         1326 g          800 t
ORIGIN      
        1 gacccctctt acgtcagatg ggagtgtaag gaggaccaca actctcttgc gaccaccacc
       61 ggttgcacgc ggccaattct gctgccggtg ttgcttgtca atcacagacg gggcggtgga
      121 gcgagaaaag gttgagcggg agacgtgtgc ttcttctgcg ttctttttgg agaggccaga
      181 gcgaccaaat ctcaacaatt tgagatggtc attttcgctt tagtcgcgtc ggatcggcag
      241 gtcagtgcct cgcaaattgc aatctgcgcg cccatactgt tgctgaaaaa aaactgctag
      301 cgtgccgatg aaaaaagcga gcttttgatg aggtacgagc ggcaacgcct gaacgacact
      361 cgcatagacc gcgacgggat acatggcaaa gccaggatcc acaacggcag gggctccatc
      421 atccggaaga ggctgagata ccggcagtgt cggaagcgcg caggtgaacg cagttctgac
      481 cgattaaatg ttttccttca cggctgtgag gcccggccaa tccggacatt tcgtgtgcag
      541 tagagggcgg gcgcagtgct cagccgcatt ggcggtacca caattttcgc cgcgctgtcg
      601 aaccgaggaa gcgaagccag tgtgacggaa gcgaggagtg gctaattggg gtggatctat
      661 tgtagctcgc cgctctgcac gcacacggca aggctaaacc ttgttagcct catccgcgag
      721 ttcggtcggc ctgcacccat ccgcacaccg tcatctgtga catcaaggct gtcaatctcg
      781 aacgcgatgg ctaccgaaga agcaacgcta cagggaccag atcgcgttcg gttgcgcaag
      841 cttttgactt tgatgggaaa atttggttgc gggggcagga tttgaacctg cgaccttcag
      901 gttatgagcc tgacgagcta ccgggctgct ccaccccgcg tcaccagctg tttgccgaca
      961 agccgagcga gcagggctcg gctgcggtcc gatatcaaaa gccgcccggc gggggcggct
     1021 tcggtgtaag gcttatatag cacgagaatc ggagatgtga agcccatggg acgcaaatgg
     1081 ggaactgcct tgctgcagcg tcaggactgc ctgccggcaa cctccccatc cggagccgca
     1141 ggtaaacgct tgctgcgcag gatgaagtgt aggttgcggg catagatgaa caggcccatg
     1201 ccctggccca acatgaacac agggtcgaga cggtagatcg cgtacgcgaa gagcagggcg
     1261 ccgccggcca gactgaaata ccagaaggtc tcaggcacga tggagcggcg agcgcgctca
     1321 ctcgcgatcc attgcacgat gaagcgcatg gagaacatga gctgagccac gaagcccacg
     1381 gccagccaca ccttctccgc tgtgggagtg gaggtccacc aggccgcagt cttggtcgcg
     1441 gcagttgcaa gaaaatccat tgatcccccg atgcgtcgcg tcggatcctg ggcttaccgc
     1501 tcagctcttg tcgccggtct tacgcaccag gccatcactc cgcaatttca accgccggcc
     1561 gaggtcagag atgtggcgtc cctccggcac atcctcgacg atctcgggaa cacgggtgcg
     1621 ccggcgcagc cagctcacac cgattagatc ataaaggccg atcaatgcgc ggctcaggtt
     1681 gttgtacttg gactggcctg cctgccgcgg gcgatcgttg accggcacat aggcgacctc
     1741 gtggccgtag gtcaggaaca gcgccggcag atagcggtgc atgctggtga agaacggcag
     1801 ccgcaggaaa acctcccgcc aatagacctt aatcccgcag cccgtatcag ggcaggaatc
     1861 gcggagcacg gcatccctga tccagttcgc cgccttggac gcgaagcgct tggcaccctc
     1921 cgccttgcgg tctgtgcgta taccgccgac gagcgcaggg ccggatgttc ccggcggggc
     1981 aagccgggcg agcagccgag gaatgtcgcg gggatcgttc tgtccatcgc cgtccatcgt
     2041 agcgatgatc gggctcgagg cggccaggat gccggttctc atcgcggcgc tttgtccggc
     2101 acgcgtgccg tggcggagat agcgcagccc cgggcagcgg ccggaggcga tcagtgcctt
     2161 gacctcttcg ccagtggcat cctcgctggc atcgtcgacg acgatgacct ccgccagata
     2221 gtcggcgggg accgtagcat acgtctcctc gacgaggcgg ccgatgttgc cggcctcatt
     2281 gagggcggga atgaccactg agatgcgcat gagcgatgtg atgcccccgt cgtggcggcc
     2341 atggcacgcg agttgtgcct taaggcacgc tcgaccgaag gtatcagggt ttgcgagcgt
     2401 cgctcaacca tgtaaatggg caacggcggg ctgcacggcc tgctcgcaga tgtcacgggg
     2461 gagcggcaga ccatgacggt gcagcccgga ctagaaggcg accgggtggc cggtgaggcc
     2521 tggttcccag cactcgtcgc ggtgcttggc ggactgctcg tggtccggat ggtggctctg
     2581 gcgttcaacg ccaccgacct gttcttcgac gaagctcagt actggtcgtg gagcctggag
     2641 ccggccttcg gctactattc caagccgccg ctgatcgcgt gggtgatccg cgtggccact
     2701 gaggcatgcg gccactcgga gttctgcatc cgcttgccgt cgccgatcat ccattcggca
     2761 acggcgctcg tgatctatgc tgtggccgcg cggctctatg atcggcgtgt ggggttctgg
     2821 tccgccgtcg tgttcgcgac gctgcccggt atttcgctct ctgcaggcat tatctcaacg
     2881 gatgtgccgt tgctgttttt ctgggccgtc gcactttatg cgctcgtgcg gctcctcgat
     2941 tccgaccgct ggtggcccgc ggtgctgctg ggcgtggcgc tcgggcttgg gctcaatgcc
     3001 aagtatgcga tggtgtactt ccttctttcg gcggccgcct acatggtggc cgttcctgaa
     3061 aagcgccgcc ttctccatga tgcccgagcc tatgcagcac tcggcatcgg cctgctgctg
     3121 atcgcgccca atctcgcgtg gaacgctgcg aacagcttcg ccaccttcgc tcatacagcc
     3181 gacaacgcca attggggcgg aacgctcttt catcccgaga aagcactgga gttcttcggc
     3241 gcgcagttcg gcgtgtttgg gccgatcctc ttcggctcgc tgctggtgat cacctggcgc
     3301 gcctggcagg atgggctgcc agagcccgat cgtttgctgc tggcatttgc gctcccagtg
     3361 attgttcttg tcaccatcca ggcgttcctt tcgcgggcgc atgcgaactg ggccgctgtc
     3421 agctacgtct cggccaccgt gttggtaaca gcgacgatgc ttcgcgaagg tgcttggcga
     3481 tggttcaaga gctcgctcgc gctgcacgcg gcaattctgg tcggcattgg gcttgcgacg
     3541 gcagcggctg gatcgttcag tctgcctgta ggtggtgacc ccttcgcacg gacactgggt
     3601 tggcgggaac tcgcgcagga gaccgcgaca gtgctggccg aagcgcggca atccggacgg
     3661 ccgttcggcg ccgtgattgc agacgaccgt gcgatcacgg cagagctgct ctattacatg
     3721 cgtgatgagc ctacatcggt gttcgcctgg cgcgagccag gcgtgcgtcc aaaggatcac
     3781 tacgagctga cccgtgctta ccaaagaggt tcacccgaac cggtgctgtt ggtcagcttt
     3841 cgtcgcggcg cagagagggt gagcagcaga tttgccgtcg tcgagctgat tgttgagcgc
     3901 gaggtgccgg ctggcgcagg gccgggccgg aaagtcgcgt tctacaggct cgagggcttc
     3961 gcgggcgttg agtaagcgcg cacgggatct gtgccgcagt cctcaagtcg ccgttagaat
     4021 gactgccgga gcccagcgcg gacggatatt ggccggcatc gggcggcgga gc
//
LOCUS       DASUMG010000383         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_933681, whole genome shotgun sequence.
ACCESSION   DASUMG010000383 DASUMG010000000
VERSION     DASUMG010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933681"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFX46_06305"
     CDS             <1..632
                     /locus_tag="VFX46_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600226.1"
                     /translation="QRVALARAIVPRPSVMLMDEPFSGLDVQLRDTMQEETLALLRET
                     RATCMIVTHHPEEAMRLGDRIAVMRRGKIIQVGKAEDLYHNPAELFVARLFSEINEVP
                     CRVAGGALHTPLGTFPVPGLREGEQAILCLRQRGFRLLPPGQGLPGRILDVRFLGDLG
                     RVEIAAQGFDAPLKARVRESEGWKRGDEVSIEIDPTRVLVFPAENLETD"
     gene            757..993
                     /locus_tag="VFX46_06310"
     CDS             757..993
                     /locus_tag="VFX46_06310"
                     /inference="COORDINATES: protein motif:HMM:NF001940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX5600227.1"
                     /translation="MGATSIWHWIIVLLIVVLLFGRGKISEIMGDVAKGIKSFKKGMA
                     EDDTAAQPEQGPKVIEQSSVKPVEQTGTESSKAS"
     gene            1085..1552
                     /gene="tatB"
                     /locus_tag="VFX46_06315"
     CDS             1085..1552
                     /gene="tatB"
                     /locus_tag="VFX46_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEX5600228.1"
                     /translation="MFDIGWGELLVIGVVALLVVGPKELPALLRTIGRYVGMIKRQAN
                     EFRAQFDAAMREAELEQLRKEVAALKNEAESSLRGAEQSVRSELQKAKTELDSAVAGP
                     EAPSSAVQPQAASSTPASVNGSGQGNGFNGSAVDQPRVAETSQSAKSESVSGG"
     gene            1549..2364
                     /gene="tatC"
                     /locus_tag="VFX46_06320"
     CDS             1549..2364
                     /gene="tatC"
                     /locus_tag="VFX46_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX5600229.1"
                     /translation="MTTDTHQTQRPGHDEVEASKAPLMDHLMELRQRLIYCLVAVFVG
                     FVVCFAFAEEIYNLLIVPYRWAAGADSKIDMIYTAPHEFFFTQVKLALFGAIFLAFPV
                     IAIQVYKFVAPGLYYNERDAFRPYLIATPILFVIGACVVYFIVMPLAMTFFLSMEWKT
                     GDVQIHLQAKVSEYLSLIMALILGFGAIFQLPVVLTLLARAGLLEAQTLREKRRWAIL
                     GVFVVAAILTPPDPISQISMALPTILLYELSILAVDRVAKARRAQEEAARSAA"
     gene            2488..>2920
                     /locus_tag="VFX46_06325"
     CDS             2488..>2920
                     /locus_tag="VFX46_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX5600230.1"
                     /translation="MFDIKWIRENPETFDEGLRKRGLEPLSARLLAIDEERRKVLTSL
                     QEAQSRRNAASKEIGKAKAAKDEAKAAALMAEVNALKSEQESSAVRLDEIQPELQALL
                     LAVPNLPHASVPVGEDESANVEVRRWSPQGGAGSAASPLGFA"
BASE COUNT          534 a          880 c          965 g          541 t
ORIGIN      
        1 aacagcgagt cgcccttgcg cgtgccatcg tgccccggcc gtcggtgatg ctcatggacg
       61 agccgttctc cgggcttgat gtacaactcc gcgacacgat gcaggaggag acactggcgc
      121 tgctgcgcga aacccgcgcc acctgcatga tcgtgaccca ccaccccgag gaagcgatgc
      181 gccttggcga ccgcattgcc gtcatgcggc gcggcaagat catccaggtc ggcaaggcgg
      241 aagatcttta tcacaatcca gcggagttgt tcgtcgcgcg cctcttttcg gaaatcaacg
      301 aggtgccgtg ccgggtcgcc ggaggcgcac tacatacgcc cttagggaca ttcccggtgc
      361 ccgggcttag agagggtgag caggcgatcc tttgcctacg ccagcgcggc ttccggctgc
      421 tgccgccggg acagggactt ccaggtcgca tcctcgatgt caggttcctc ggcgacctgg
      481 ggcgggtgga aatcgcggct caaggcttcg acgcgccgct caaggcacga gtgcgcgaaa
      541 gtgagggttg gaagcgcggc gatgaggtct caatagagat tgatcccact cgggtgcttg
      601 tgtttcctgc ggaaaatttg gaaaccgact gattttcaga agcttaggct ttgatgtcgc
      661 ggtctggcgc acgggttgct gctggccgcc gtttctgcga tagtgcaatc aaactttgtg
      721 gagcgcggcc gcaggctgca aaggagttcc agacacatgg gcgcaaccag catttggcac
      781 tggatcatag tccttttgat cgtcgtgctg ctgtttggcc gcggcaagat ctccgagatc
      841 atgggcgacg ttgccaaagg catcaagagc ttcaagaagg ggatggcgga ggacgatacc
      901 gcggctcagc ccgaacaggg tccgaaagtc attgagcaat cctcggtcaa gcccgtcgag
      961 cagaccggga ccgagagcag caaggcgagc tgagctgccg acggcccatc agaggccaag
     1021 cccgccggat ggtgagctgg gcggcttgcg gtcgtctggc gggtgagggc gtgggcgaag
     1081 agacatgttc gatatcggct ggggcgagct actggtcata ggtgtcgtgg cgcttttggt
     1141 cgtcgggccg aaagagctgc cggccctgct gcgcacgatc ggccggtacg tcggcatgat
     1201 caagcggcag gcgaacgagt ttcgcgctca gttcgacgcg gccatgcgcg aggccgagct
     1261 cgagcagttg cgaaaggagg tcgcggctct gaagaacgag gccgagtcca gcttgagagg
     1321 cgccgagcag tcggtgcgaa gcgagctgca gaaggccaag actgagctcg atagcgcggt
     1381 ggcaggaccg gaagctccat cctcggcagt gcaaccgcag gcggcctcgt ccacgccggc
     1441 gagcgtcaat ggctccggcc aggggaatgg gttcaacggg tcggcggttg atcagccgcg
     1501 cgtggccgag acgagccaga gcgcgaagag cgaaagcgta agcggcggat gaccacggat
     1561 acgcatcaga cacagcgtcc cggacacgac gaggtcgaag cctccaaggc gccgctcatg
     1621 gaccatctca tggagctgcg ccagcggctc atttattgcc tagtggctgt tttcgtcggg
     1681 ttcgtcgtct gcttcgcctt tgcggaagag atctacaacc tcttgatcgt gccctatcgc
     1741 tgggcggccg gcgccgactc caagatcgac atgatctata cggcgccgca tgagttcttc
     1801 ttcacgcagg tgaagctggc cctcttcggc gcaatattcc tcgcgtttcc cgtcattgcc
     1861 attcaggtct acaagttcgt agcacctggg ctgtactaca acgagcgcga tgccttccgg
     1921 ccttacctga tcgccacgcc gatccttttc gtgatcggag cctgcgtcgt ctatttcatc
     1981 gtcatgccgc tcgccatgac cttcttcctc tcgatggagt ggaagactgg cgacgtgcaa
     2041 atccacctgc aggcgaaggt cagtgagtat ctgtcgctga tcatggcgct catcctcggg
     2101 tttggcgcca tcttccagct cccggtcgtc ctgacgctgc tggcacgggc cggcctgctc
     2161 gaagcccaga ctttgcgcga gaagcggcgt tgggccattc tcggcgtgtt cgtggtcgct
     2221 gcgatcctca cgccgcctga tcccatcagc cagatctcca tggcgctacc gacaatcctc
     2281 ctctacgagc tttcgatcct cgcggtcgat cgcgtggcca aggcgcgccg ggcgcaggag
     2341 gaggccgcgc gcagcgcggc ctagctgagc acttagccga tttgactcgt tggacttgcg
     2401 gcgttgccac ggctggacca gcctgtggca agatcctaat aggtcggcgt ctcccgaatt
     2461 ggtcgattca gcgcgagggc tgcgcccgtg ttcgatatca aatggatccg cgagaacccc
     2521 gagacctttg acgaaggcct gcgcaagcgc gggctcgagc cgctttccgc gaggctcctg
     2581 gcgatcgacg aggagcggcg gaaggtgctg accagccttc aggaggcgca atcccgtcgc
     2641 aatgcagcct cgaaggaaat cggcaaggcc aaggccgcca aggacgaggc caaagcggcg
     2701 gcgctgatgg ccgaggtcaa tgcgctcaag agcgagcagg aatcctcggc ggtgcgcctc
     2761 gacgagatcc agcctgaact gcaggccctg ctgctggcgg tgcccaatct gccgcacgcc
     2821 agcgtgccgg tgggtgagga cgaatcggcc aatgtcgaag tgcggcgctg gagcccgcaa
     2881 ggcggcgccg gcagcgctgc ctcccccctg ggctttgcgc
//
LOCUS       DASUMG010000384         3917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_532295, whole genome shotgun sequence.
ACCESSION   DASUMG010000384 DASUMG010000000
VERSION     DASUMG010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532295"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFX46_06330"
     CDS             complement(<1..197)
                     /locus_tag="VFX46_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HEX5600231.1"
                     /translation="MAENTLHTQPDSGFHAILTQHRSLSPTGFMILMAIVGCSSFVLG
                     MVFLSMGAWPVFGFLGLDVLLL"
     gene            234..1046
                     /gene="nth"
                     /locus_tag="VFX46_06335"
     CDS             234..1046
                     /gene="nth"
                     /locus_tag="VFX46_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX5600232.1"
                     /translation="MDAKPKFSSLRRAGPRPAQSPHRAKLKPAVVHEIFRRFAAANPE
                     PKSELEYHNPYTLLVAVVLSAQATDAGVNKATRSLFKIADTPEKMLALGEERLREAIK
                     SIGLYRNKAKNVIALSEKLVREHGGQVPRDRESLEKLPGVGRKSANVVLNVAFGEPTM
                     AVDTHVFRVARRIGLADGPTPLAVEQQLLKIVPPEYARHAHHWLILHGRYVCKAKKPE
                     CPRCLINDLCLYEPKTLPSELRLSGNASAPGASGVAGTDAGKGSSSKLRRKS"
     gene            complement(1073..2653)
                     /locus_tag="VFX46_06340"
                     /pseudo
     CDS             complement(1073..2653)
                     /locus_tag="VFX46_06340"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
     gene            complement(2766..3737)
                     /locus_tag="VFX46_06345"
     CDS             complement(2766..3737)
                     /locus_tag="VFX46_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX5600233.1"
                     /translation="MSDPSLQPADGKQEMMQLPPAPDPVSIDFSAIEGLPKSAQRRLW
                     SISLGAALAAHALILLLVTWIPGLRLVGAGGTDFEAIGVDIVSAEALESRLAAVEVGR
                     ATRDSSVHVTEGSQDPTQASMATADQKPSESDQEENAAGPKPDLVIPDAREHEEPDEP
                     SEVALAIADKEPERPDNRESETDPNRKTEEASEPESAAPSKPSEASVAAEEGGAAARG
                     IDIVETPGQQAAVASAGVANEYAKALIETLARNKPRATAGVRGTVRIGFTVNRFGEVQ
                     EAHILQSSGRRVLDEAALAAVRSAKFPAPPPSLADSSLRYEVPYIFR"
BASE COUNT          768 a         1218 c         1176 g          755 t
ORIGIN      
        1 agcaggagaa cgtcgaggcc gaggaacccg aataccggcc acgcgcccat ggacaagaag
       61 accatgccga gcacgaagct ggagcagccg acaatcgcca tcaggatcat gaagcccgtc
      121 gggctcagcg aacggtgctg ggtaagaatt gcgtggaatc ccgaatcggg ttgcgtatgg
      181 agggtattct cggccatgac ttcctgacct tacgccactc tcctcggata actatggacg
      241 ccaagccgaa gttctcttct ttgcgccggg ctgggcctcg tccggcccaa agcccgcatc
      301 gtgcgaagct caaacccgcc gtcgtccatg agatctttcg ccggtttgca gcggccaatc
      361 ccgagccgaa aagcgagctg gagtatcata acccatatac cctactcgtt gccgtggttc
      421 tctcggcgca ggcgaccgac gccggagtga acaaggcaac gcgctcactg ttcaagatcg
      481 ccgatacgcc ggagaaaatg ctcgcccttg gcgaggagcg tctgcgtgag gcgatcaaat
      541 cgatcggatt gtaccgtaac aaggccaaga acgtcatagc cctgtcggag aaattggttc
      601 gcgagcacgg aggccaggtt ccgcgggatc gtgagtcgtt ggaaaaactt ccgggtgtcg
      661 ggcgcaagtc ggcgaatgtc gttctgaacg tcgccttcgg cgagccgacg atggctgtgg
      721 acacgcatgt ctttcgcgtc gcgaggcgta ttgggctcgc ggacggtcca acgcctctcg
      781 cggtggagca gcagctcttg aagatcgttc cgcccgagta cgccaggcac gctcatcact
      841 ggctcattct gcacgggcgc tatgtctgca aagccaagaa gccggagtgt ccgcgttgcc
      901 tgataaatga cctttgtctc tacgagccca aaacgttgcc atcagagcta agattatcag
      961 gcaatgcgag cgcgcccggc gcttctggcg tggcaggaac tgatgccggt aaaggctcat
     1021 cgtcgaagct gaggcgaaaa tcctgagtca tgtgcaacat ggcgcgttct gccagcgctg
     1081 tgatcgtcag cagcggattc acgccgagcg accggggtat caccgagcca tccatcacat
     1141 acaatcccgg aaacacgtcg ttagagccag ccccaggcct gccgtcgaac acttgccctt
     1201 tatgattgac cactccctca ttgcggtcgc gccccatggc gcaaccaccg agcgggtgtg
     1261 ccgtcgctgg ttggtggccc atcaaggtgc ttgagagcgg gttcttaacg tactgccccc
     1321 caacctcggt gacgagcttg gaaaggatgg catccagttt ctgatagacc ggctcgtcct
     1381 tcgctgtggg ccaagagacg gtcacccgct cgccatccag aaccagacgc ccgccggagc
     1441 tgtcgtggga gactgcgaag aatgtctgca aacgcgagaa cgggcccttg tagaccccgc
     1501 tgatgaggct ttgcacggct cccaggagcc gcccgttcgg catgaacaac accggcaata
     1561 tcggtgcgag agcagagggg aggacgcctt cttgtaccgt tagctcgttg gcgaggtcgg
     1621 tgccatcccg cagctcgatc tggcctgaga cgcaggcgcc aatacacgcg tcgagcgcaa
     1681 cacgcggtgg atgaccgacg cccactgcat ttatcggctg ccgtgctccg tagccgaagg
     1741 cgatgatgtc tccgttcgca gaaaatcgct cgcccagccg tggtgacagc ggtagcccgt
     1801 cagcttgcga tcgaaggagg atttccgtcg agccgagcgt gccggcagcg aggatgacca
     1861 tgtcggccga catagtcagc tggacgtcgc cgttaccctt atcgagacgg gcaatgcgca
     1921 cgtgccacag tccatcgcta tcgcgggcaa gcgacaagac cttcgcttcg gtaaagagat
     1981 gagcaccgtg gcgcaccgca tcgggcaggt aggtcagggc gaccgtgttc ttggcgccca
     2041 aattgcagcc gccgcagcag tctccgcatc gggtgcacgc cggctgggcg atgccggcga
     2101 cattcaccgc ttcctcaaag ttgacgacta cgggcgctgc gaccggctcg accccaagcg
     2161 ttcggcctgc cgtcgctaac gcctggaact tggtgtgctc agctgcccta ggatcgcgcg
     2221 ccgggcggag ccagcgccgg gcacgacgaa aaccttctgc gagaaggccg tcttgtgcga
     2281 tctggccggg ccagatcgcg tcggcgaaga cacgcggatc gggttccaag gcgacacccg
     2341 cattcaccag cgatccgccg cccagcccgc acccgacgag cacgtgcatg tcctcgccga
     2401 gccggaaatc ataaagggca gtttcgcggc ccatattcat ccgctttccc cttacctgca
     2461 gctcattgcg caggtcggga aagcgtttcg ggaactcgcc tgtcaggaac tcacggccac
     2521 gctcgaggac ggcaacgcgt ttgcccgcgc gagcaaggcg tgatgctgca acgccgccgc
     2581 cgtagccgga acccacaatg atcacatcgt accggtcctg tctgaaatcg agattgcgag
     2641 caagccgcgc cattttgttt cctcagtcag aggacctcgt gaaagcagtt cagccgtctg
     2701 aatagacaaa cgtcggtccc ttgtccgctt cttcagtaga acgacgtgaa gaattgctcg
     2761 taatatcaac ggaagatgta gggcacctcg taacgtagcg agctgtccgc tagggagggt
     2821 ggcggcgcag gaaactttgc acttcgaact gcggccaggg ctgcctcgtc aagcacgcgc
     2881 cgtccacttg actgcaaaat gtgcgcctcc tgcacttcgc caaatcggtt cacagtgaag
     2941 ccgatccgaa ctgttccgcg aacaccagcg gtcgcgcgcg gcttgttccg cgccaacgtc
     3001 tcgatcagcg ccttggcgta ctcgttggcg actcccgcgc tggctacggc agcttgttgc
     3061 cctggggtct cgacaatgtc gatgccgcgg gccgcagctc ctccttcctc ggcggctacg
     3121 ctggcctcgg atggcttgga tggagcagcc gattcgggct cggaagcctc ctcagtcttg
     3181 cgatttggat cggtttcgga ttcccggttg tccggccttt ctggctcctt gtcggcgatt
     3241 gccagggcaa cttctgatgg ctcgtccggc tcctcgtgct ctcttgcatc tgggatgacc
     3301 aggtcaggct ttggaccggc tgcattctct tcttggtcgg actcgcttgg cttctgatcg
     3361 gccgttgcca tgctggcttg agtcggatct tgggagcctt ctgtcacgtg aactgagctg
     3421 tcgcgtgttg cacgacccac ttccaccgct gcgagtctgc tttccagagc ttccgcggac
     3481 acgatgtcca cgcctatggc ctcgaaatct gttccaccgg cgccgaccag ccggaggccc
     3541 ggaatccagg ttacgagcaa caaaatcaat gcgtgtgccg ccaaggccgc accaagactg
     3601 atggaccaaa gccggcgctg agcagacttc gggaggccct cgatggccga gaagtcgatc
     3661 gaaacgggat caggcgctgg cggcagctgc atcatctctt gcttgccgtc agcgggttgg
     3721 aggctcgggt cgctcatccc cggttccgga acagtcctac gtcagcctgc cgaaaacaat
     3781 tataccaacg tcagctcaat gtcccattgc tggacaacgt gtacgcgagt ggtctgctca
     3841 catgaacgcc agtcgccgta cctcacctga ggcagccaga gcgcaatctc agggacgatg
     3901 atgatcagca ctaggcc
//
LOCUS       DASUMG010000385         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_131341, whole genome shotgun sequence.
ACCESSION   DASUMG010000385 DASUMG010000000
VERSION     DASUMG010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131341"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1576
                     /locus_tag="VFX46_06350"
     CDS             134..1576
                     /locus_tag="VFX46_06350"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX5600234.1"
                     /translation="MQKPVISLLRSQCYVDGRWVGEPALPVIDPATGEEIAKVPMMSA
                     EDTRAAIDAAERALPEWAGMLAKERSKILRRWYELQMEHADELALLMTTEQGKPLAEA
                     KGEVVYAASFVEFYAEEAKRIYGETIPTFKKDARIVCIKQPVGVVAAITPWNFPAAMI
                     TRKVAPALAVGCTVVCKPASETPLTALALAELAERAGVPKGVFNVVTGKASVIGGELT
                     SNPKVRMLTFTGSTEIGKILIEQCAKTVKKVGMELGGNAPFIVFDDADLDKAVQGAMA
                     SKFRNAGQTCVCANRIYVQDGVYDAFAEKLAAEVAKLKVGRGTEPGVTTGPLINAAAV
                     EKVEEHIADATSKGAKVIQGGKRHALGGNFFEPTVITGVTSEMKVAREETFGPVAPLF
                     RFKTEEEAIKLANDTEYGLAAYFYSRDIGRVWRVGEALEYGIVGINEGIISSEVAPFG
                     GVKESGLGREGSHHGVEEFLEIKYMLMGGV"
     gene            1576..>2167
                     /gene="rpiA"
                     /locus_tag="VFX46_06355"
     CDS             1576..>2167
                     /gene="rpiA"
                     /locus_tag="VFX46_06355"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX5600235.1"
                     /translation="MTDALKLKAAEKALEFVEPGMKLGLGTGSTASKFIDLLGAKVKA
                     GLDIIGVPTSEATRAQAAALGIRLTTLEEDPVLDLTIDGADEIDDALRLIKGGGGALL
                     REKIVAAASRRMIVIADSSKRVPVLGAYPLPVEVVRFGVAATRKMIEELAADAGYTGH
                     VHLRMGYDGQPFVTDGGNYIFDCAFGRIEKPEVLDQV"
BASE COUNT          420 a          654 c          718 g          375 t
ORIGIN      
        1 acagacttag gcgatccctc ccgtgctgtc accggtttcg accgtgcttg ccagcagttc
       61 tccagggtct tagatcaatc ccgcaggctg ctggtgcgat agtgtggccc caattcccag
      121 aaggagcacc ccaatgcaaa agcccgtcat cagtcttctc cggtcgcaat gttacgtcga
      181 tgggcgctgg gttggtgagc cggcccttcc cgtgatcgac cctgcaacgg gcgaggagat
      241 tgccaaggtc cccatgatga gcgccgagga tacgcgcgca gcgatcgacg ctgcggagcg
      301 ggcgctgccg gagtgggccg gcatgctggc caaggagcgc tcaaaaatcc tccgtcgctg
      361 gtatgagctg cagatggagc acgctgacga gctggcgctg ctcatgacga cagagcaggg
      421 caagccactc gccgaggcca agggtgaggt cgtctacgcc gcatcgttcg tggagttcta
      481 tgccgaggaa gcgaagcgca tctacggcga gacgatcccg accttcaaga aagacgcccg
      541 catcgtctgc atcaagcagc cggttggcgt cgtagcggcc attacgccgt ggaacttccc
      601 ggccgcgatg atcacccgca aggttgctcc tgcgctcgcg gtcggttgca cggtggtatg
      661 caagccagcg tccgagacgc cgctaaccgc gctggcactg gccgagctcg ctgagcgggc
      721 cggcgtgccg aagggcgtct tcaacgtcgt caccggtaaa gccagcgtca tcggcggcga
      781 gctgacgtcc aacccgaagg tgcggatgct gaccttcacc ggctcgacgg agatcggcaa
      841 gatcctcatc gagcagtgcg ccaagacggt gaagaaggtc ggcatggagc tgggcggcaa
      901 cgcgccgttc atcgtcttcg acgacgccga tctagataag gcggtgcagg gtgccatggc
      961 ctccaaattc cgcaacgcgg gccagacctg cgtctgcgcc aaccgcatct acgtgcagga
     1021 cggcgtctat gacgcgtttg ccgagaagct ggcggccgag gtggccaagc tgaaggttgg
     1081 ccgcggcacc gagccgggag ttaccaccgg gccgctcatc aacgcggcgg cagtggaaaa
     1141 ggtcgaggag cacatcgccg atgcgacctc caagggcgcc aaggtgatcc agggcggcaa
     1201 gcggcatgcg ctcggcggca atttcttcga gccgacggtg atcacaggcg tgacctcgga
     1261 aatgaaagtc gctcgcgagg agaccttcgg gccggttgca ccgctgttcc gcttcaagac
     1321 cgaggaggag gccatcaagc tcgccaacga taccgagtac ggccttgccg cctacttcta
     1381 ctcgcgggac atcggccgcg tttggcgcgt cggtgaagcg cttgaatacg gcatcgtcgg
     1441 catcaatgag ggcatcatct catccgaggt tgctccgttc ggcggcgtga aggagtccgg
     1501 cctaggccgc gaaggctcgc accacggcgt cgaggagttc ctcgaaatca agtacatgct
     1561 gatgggcggc gtctgatgac ggacgccctg aagctcaaag ccgccgagaa agcccttgag
     1621 tttgtcgagc ccggcatgaa gctcgggctc ggcaccggct caacggcatc caaattcatc
     1681 gatctgctgg gcgccaaggt gaaggcgggc ctcgacatca tcggggtccc cacgtccgag
     1741 gcgacacggg cccaggcagc ggctctcggc attcgcctga cgacgcttga agaggatccc
     1801 gtcctcgatc tgaccatcga tggcgctgac gagatcgacg acgccctgcg gctcatcaag
     1861 ggcggtggcg gggcgctgct cagggaaaag atcgtcgcgg cagcgtccag gcggatgatc
     1921 gtgattgctg attcctcaaa acgtgtgccg gtgctgggag cctatcccct gcctgtggag
     1981 gttgttcgtt tcggcgtggc ggcaacgcga aagatgatcg aagagctggc agccgacgcc
     2041 ggctacacag gacatgttca tctcaggatg ggctacgacg gccagccgtt cgtaacggac
     2101 gggggcaatt acattttcga ctgtgccttt ggccggatcg agaagccgga agttctcgat
     2161 caggtgc
//
LOCUS       DASUMG010000386         2591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_852925, whole genome shotgun sequence.
ACCESSION   DASUMG010000386 DASUMG010000000
VERSION     DASUMG010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852925"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VFX46_06360"
     CDS             <1..593
                     /locus_tag="VFX46_06360"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX5600236.1"
                     /translation="LPRDARARRFVLGLILAFLPAAAVGVALHGFIKTVLFETPMLVC
                     ITLIVGGVVLLYVDQIAFKPRFKDVMEFTPSLALKIGLCQTAALIPGVSRSGATIVGA
                     MLLGTDKRSAAEFSFFLAMPTMAGAFAYDLYKNWLILQPDDLVGIAVGFVAAFVAAVI
                     VVRMLLRFVSRYGYALFGWWRILVGGLGLGALAVLG"
     gene            complement(608..1078)
                     /gene="ptsN"
                     /locus_tag="VFX46_06365"
     CDS             complement(608..1078)
                     /gene="ptsN"
                     /locus_tag="VFX46_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEX5600237.1"
                     /translation="MDLGDLLTPESVIPSLKAKSKKQALQDLSQRAAELTGLQARDLF
                     DTLLQRERLGSTGLGRGIAIPHVKVKGLKKIVCLFARLDEPIEFESLDDEPVDLIFLL
                     LAPEHASGDHLKALARISRLLREPQTAGRLRAAKDPAALYAILTETATSSSEAV"
     gene            complement(1804..>2591)
                     /locus_tag="VFX46_06370"
     CDS             complement(1804..>2591)
                     /locus_tag="VFX46_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX5600238.1"
                     /translation="HNLPALRKVVGVEMDDLLDMIAEIKRLNPKPGLQFGSVQVLPVI
                     PDVFVRPTKDGSWRVELNSETLPRVLVDRTYYARVSRTSRDAREKSYLLDCLQSANWL
                     VKSLDQRARTILRVAEEIVRQQDAFFSYGIEHLKPLNLRKVAEAVAMHESTISRVTSN
                     KYMATPRGIFEMKYFFTSAIPAAGEGDAFSSEAVRHRIRQMIEAETFETVLSDDMIVD
                     RLKAEGIDIARRTVAKYREAMRIPSSVQRRREKRLASGARNVA"
BASE COUNT          467 a          812 c          755 g          557 t
ORIGIN      
        1 acttgccgcg cgacgcacgt gcccgccgct tcgtcttggg cctcatactg gcctttctac
       61 cagccgcggc agtaggagta gccctgcacg gtttcatcaa gacggtgctc ttcgagacgc
      121 cgatgctcgt ctgcataacc ctgattgtcg gcggcgtcgt ccttctctac gtggaccaga
      181 tcgccttcaa gccgcgcttc aaagacgtga tggagttcac gccttccctg gccctcaaga
      241 tcggtctgtg ccagaccgcg gctctcatcc ccggcgtatc ccggtccggc gccaccatcg
      301 tcggcgccat gctgctcgga acggataagc gctcggcggc cgagttttcc ttcttcttgg
      361 caatgcccac tatggccggt gcttttgcct acgaccttta caagaactgg ctgatcctgc
      421 agccggatga ccttgttggg atagccgtcg gcttcgtcgc cgcatttgtg gccgccgtta
      481 ttgtcgtgcg catgctgctg cggtttgtga gccgctacgg ctatgccttg ttcggctggt
      541 ggcgaatttt ggtcgggggg cttgggctcg gcgccttggc agttctcggc taaggcgcca
      601 ccgcccttca gactgcttcg gacgacgacg tcgccgtctc tgtcagtatg gcataaagtg
      661 ccgccgggtc cttcgctgcg cgcaagcggc cggcggtttg cggctcgcgc agcagccgcg
      721 agatgcgtgc aagtgccttg aggtgatctc cgcttgcgtg ttctggggcg agcagcagga
      781 agatcaagtc caccggctcg tcatccagtg attcgaactc gatcggttcg tccaagcgag
      841 cgaacaggca cacgatcttc ttcaagcctt tgacttttac gtgcgggatc gcaattcctc
      901 ggccgaggcc tgtcgaaccg aggcgttccc gctgcaacag ggtatcgaag agatcccgtg
      961 cctgaagtcc tgtgagctcg gcagcgcgct ggcttagatc ctgtagcgcc tgcttcttgc
     1021 tctttgcctt cagggacgga atgacgcttt ccggcgtcag caagtcccca agatccatga
     1081 tgaagtcatc ccttcggggt gagagcctcc agtacgaatc aacccgggcg gcgcatgcac
     1141 gagcgcgcgc cttgcccagg cgagaagcgc tcagcgttcc ggcatcggcg atgggcgcgt
     1201 gggctcgcct tgtgcggcgc agcaattgct gcttcgggga tatgatatgc aagtccaata
     1261 gcccaagcct cgcgaaacac caaggaacat atgtctggtc aagtccgacc gcatcaaggc
     1321 cgcagcaaac gcgatgcgac atttgtccgg ccagcctggc ttaaacgcat cgcgcaatat
     1381 ttgcgccaac tcggagacgg tacctcatca tcggtgctcc tcccccgacg gcgttgatcg
     1441 atatcggtta ccctgctcgg cagaagcatc gtgaactcgg gacattggcg attgctagtg
     1501 caatcagccc aagaacaagg ctgcggcacc tcgctgccat cgctgtccgc caacctgtca
     1561 gctcagcgaa tgcgcggcct tacccctcag ctgagcgctt cgctcaactt ggactctacg
     1621 atccaactgc tacggaaaat cgacacactg ggtcactggc tggatcagtt agacctggac
     1681 ctcatcagtg tcaatcgcga gtgtgtcgga acgtctcgct agtgtggagc agaaaggccg
     1741 tccgcacttg gccattcatc ggcacgcacg gagaagatct ccgtcccgag tgcctagtcc
     1801 cactcaggcg acgttacgcg ccccggacgc aagccgcttc tcccgccgcc tttgcacgga
     1861 tgaagggatg cgcatcgctt cacggtactt cgccactgtg cggcgggcta tgtcgatccc
     1921 ttccgccttc agccggtcca cgatcatgtc gtcggacaac acggtttcaa acgtctcagc
     1981 ttcgatcatc tgacgaatgc gatggcgcac cgcctctgac gagaatgcgt caccctcgcc
     2041 cgcagccgga atggcagacg tgaagaagta tttcatttcg aagatgccgc gcggcgtggc
     2101 catgtacttg ttggacgtga cccgactgat cgtcgattcg tgcatggcaa cggcctctgc
     2161 aaccttgcgc aggttcaggg gcttaaggtg ctcgatcccg tagctgaaga acgcgtcctg
     2221 ctggcgcacg atctcctccg cgactcgcag aattgtccga gcgcgttggt cgaggctctt
     2281 gaccagccag ttggccgatt gcaggcaatc caagagatac gatttctccc gagcatccct
     2341 cgacgttctc gatacgcgcg cgtagtaggt gcggtcgacc agcacccgcg gcagggtctc
     2401 ggaattcagc tcgacgcgcc agctcccgtc tttggttggc cggacgaaga cgtcagggat
     2461 tacaggcagc acctgcacgg atccgaattg caggcccggc ttcggattga gccgtttaat
     2521 ctctgcgatc atgtccagca ggtcgtccat ctctacaccg acgaccttac gcagggcggg
     2581 aagattatgg g
//
LOCUS       DASUMG010000387         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_291807, whole genome shotgun sequence.
ACCESSION   DASUMG010000387 DASUMG010000000
VERSION     DASUMG010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291807"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1483
                     /locus_tag="VFX46_06375"
     CDS             287..1483
                     /locus_tag="VFX46_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5600239.1"
                     /translation="MTREFDRTRPGTMPRPPSARSSIASFIVMDVMRAAAAREAEGKR
                     VIHMEVGQPGTPAPLAAREAVERAMRSETLGYTLALGLPSLRERIAQHYKDWYGLDLA
                     PERVVVTNGSSAAFVLAFLALFDAGDRVALPSPGYPCYRHILTALGQQPVIIEADDSS
                     RWMPTAAQIEEASVRDGIAGVLIASPANPTGTMLEPQRLREILEVCRKRGIWFISDEI
                     YHGLTYGMPAETALQHSDGVVVINSFSKYFSMTGWRVGWMVVPEQLVRTVEKLAQNLY
                     IAPPTVSQVAALGAFDGLEELEANKRVYAANRELLLAELPKAGLDRIVPADGAFYLYV
                     DVSRFTDDSLAFAKLMLDETGIAATPGVDFDEARGHRFIRFSYAGTTADMAEAAERLK
                     KWSRLK"
     gene            complement(1757..>2269)
                     /locus_tag="VFX46_06380"
     CDS             complement(1757..>2269)
                     /locus_tag="VFX46_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEX5600240.1"
                     /translation="QVVVASPAYLEAHGVPRTPADLARHNCLVLKNNWQWSFSRGDED
                     FSVKVNGRLRSNNGEILLHAALDGHGLLQTSELRVREDLAAGRLVRVLEDYEIASNSA
                     VWAVYPSSKHVLPKLRALLDFLAGWFREVEAQLPRAANRTGNGRKSEASIQLPKPSTR
                     QLQQPNAMLP"
BASE COUNT          439 a          665 c          695 g          470 t
ORIGIN      
        1 atcgttcggt tctgcagctt gcccatgaaa ctctcgctcg atctcacgga tagggatggc
       61 gactgatttt cactccaggt tggcgtactg gagaggctaa cagttgagcc ccgcgaatca
      121 atcccaagct ggcgcagtcc ccggctcgct actataagcc tgtcgctcaa agcctgttag
      181 agcggctacg aagcgagccg gactgaattg cgttttcagc gtggcaatgc ggcggcagca
      241 tggcgcgtct accgtcgccg cagggctgcc gagtacggag aaggtgatga caagagaatt
      301 tgatcggact cgcccgggca ccatgccgag gccaccctcc gcgcgcagct caatcgcgtc
      361 cttcatcgtc atggatgtga tgcgagcggc cgccgcccga gaggccgagg gcaagcgcgt
      421 catccatatg gaggtcggtc agcctggaac tccggcaccg ctcgccgcac gcgaggccgt
      481 agagcgcgct atgcggtcgg agacgctcgg ttacactctc gcacttgggc tgccgagcct
      541 gcgcgagcga atcgctcagc actacaagga ctggtacggc ctcgatcttg ctcccgaacg
      601 ggtcgtggtg acgaacgggt cctcagcggc attcgttctg gctttcttgg cgctgttcga
      661 cgcgggtgac cgggttgctc tgccgtcacc aggctatcct tgctacagac acattctgac
      721 ggcccttggt cagcagcccg tgatcatcga ggcggacgac agctcgcgtt ggatgccgac
      781 cgctgcccag atcgaggagg cgagcgtccg cgacggtatt gctggtgtgc tgatcgcgag
      841 ccccgccaac ccgactggca cgatgctcga gccgcagcgg ttgcgagaaa ttctcgaggt
      901 gtgccggaaa cgggggatct ggttcatctc cgatgagatc tatcacggtc tgacgtacgg
      961 catgcctgcc gaaacggcgc ttcaacattc ggacggcgtc gtggttatca acagcttctc
     1021 gaaatacttc tctatgaccg gctggcgggt tggctggatg gtcgttcccg agcagttggt
     1081 gcggactgtc gagaagctcg cccagaacct ctacattgca ccaccgacgg tgtctcaggt
     1141 ggctgcgctg ggagccttcg atggactgga ggagctcgaa gccaacaagc gggtgtatgc
     1201 cgccaatcgc gagcttctgc ttgccgagct gccgaaagcc ggcttggacc gtatcgttcc
     1261 tgccgacggt gccttctatc tttacgtcga cgtcagccgg tttacggacg acagcctcgc
     1321 ctttgcaaag ctaatgctcg acgagaccgg aattgctgcg acgcctggcg tcgacttcga
     1381 cgaggcccga gggcatcgct tcatccgctt ctcttacgcc ggcaccaccg ccgacatggc
     1441 cgaggccgcc gaacggctga aaaaatggag ccggcttaaa tagcagagcg gtttttagac
     1501 tgcgtgctgg tccagcggcg atccgaaatg acaatggcag ctggttccct ttgaatttcc
     1561 gcagtcatcg gaagctcatt tcagggttta tgataatgtc tcgttacaca ggttttctgc
     1621 cgctgagcct gtcacttgct gacgctggct atgctcagcg ttgaagagaa atgattgcct
     1681 atgacaaaaa agccgccgaa gatgcccgga agcgcgtggt tgagtatttc aaggcgagat
     1741 tggcaccagc cgggggctaa ggcagcatgg cgttgggctg ttggagttga cgcgtgcttg
     1801 gcttaggcag ctgaatcgaa gcttcacttt ttcgtccgtt gccagtgcga ttcgccgccc
     1861 gcgggagttg cgcttcgacc tcgcggaacc agcccgccag aaaatcgagt aaagctctca
     1921 atttcggaag cacgtgcttg gagctgggat agacagccca gactgccgaa ttgctggcga
     1981 tctcgtaatc ctcgagcact cgcacgagac gtccggctgc caaatcttct cggacgcgaa
     2041 gctccgatgt ctgcagcaag ccgtggccat caagggcagc atggagaaga atttcgccgt
     2101 tgttcgacct cagcctaccg ttcaccttga ccgagaagtc ctcgtcgccc cggctaaacg
     2161 accactgcca attattcttc agtacgagac agttgtggcg ggcgaggtct gcaggcgtcc
     2221 ggggcacgcc gtgggcttca agataagctg gcgaggccac cacgacctg
//
LOCUS       DASUMG010000388         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1094198, whole genome shotgun sequence.
ACCESSION   DASUMG010000388 DASUMG010000000
VERSION     DASUMG010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094198"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /gene="yajC"
                     /locus_tag="VFX46_06385"
     CDS             complement(<1..249)
                     /gene="yajC"
                     /locus_tag="VFX46_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX5600241.1"
                     /translation="MFITPAYAQGAPGGGSDFMLQLVPILLMFVIFYFLLFRPQQQRA
                     KQHREMVNNIRRGDTVVTSGGIIGKVTRVKDDNEIEVEI"
     gene            478..1359
                     /locus_tag="VFX46_06390"
     CDS             478..1359
                     /locus_tag="VFX46_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX5600242.1"
                     /translation="MTLSAETALLERIAAALERLAPPPAKPIDFDAAEAFVWQADPPS
                     FLPVRQVNRVPLQLLKGIEHHANLLLENTERFARGLPANNALLWGARGMGKSSLVKAA
                     HAEVRRRLGLGKDAKPDLKLVEIHREDISSLPMCLALMRENHHRFIVFCDDLSFDRDD
                     TSYKSLKAVLEGGIEGRPENVIFYATSNRRHLMPRDMMENERSTAINPSEAVEEKVSL
                     SDRFGLWLGFHHGSQDQYLEMVRGYASYHRLEIADDELVREALEWSMTRGGRSGRVAW
                     QFIQHLAGRLGQKLRTE"
     gene            complement(1391..>2201)
                     /locus_tag="VFX46_06395"
     CDS             complement(1391..>2201)
                     /locus_tag="VFX46_06395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600243.1"
                     /translation="DTAQALPPLDMNLAEDLMRRTRIWRLLQGYRDRPAAAIDKIAEA
                     LVRLSYLVARHPEIREIDINPLLADENGVIALDARVRVEDEAVNPRVPMALRPYPSEW
                     IKDLVLEGIGAVRLRPIRPEDEPLYDEFFQHVTKEDHRLRFLSAGVDLSRKFLARLTQ
                     IDYAREMAFVAISKDTGHLLGVSRFVADPDYTRGEYAILVRSDLKGRGLGWQLMQHLI
                     AYARSEGLEELFGSVLAENTTMLKMCSELGFSIQPEPGDLSVRRVVLDLTK"
BASE COUNT          421 a          717 c          662 g          401 t
ORIGIN      
        1 gatctcaacc tcgatctcgt tgtcgtcctt gacccgggtg accttgccaa tgatcccgcc
       61 cgacgtcacc acagtatcac cccggcggat gttgttcacc atctcgcggt gctgcttggc
      121 gcgctgctgc tgaggcctga ataacaagaa gtagaaaatg acgaacatca acagaatggg
      181 gacgagctgc agcatgaagt cgctgccccc acccggggcg ccttgcgcat aggcaggtgt
      241 gataaacatg acgtcagtcc ttgggccggc acaggtcgcg actaggagtc ccgcaggcaa
      301 cagctgccac gggcctgtcc gttaggttgc ggcgcgacta tagtggtccc tctctctttg
      361 gcaagatgcc tggtcgcagc gggactttga gccggtgacg agacggcttg tctcgactat
      421 agaggcgacc ggcgaatccg ccctcccggc tacagacaca cccgcactag gattgatatg
      481 acgctttctg ccgaaactgc ccttctcgaa cgtatcgcag ccgccctgga gcgactggcc
      541 ccaccccctg ccaagcccat cgacttcgat gcggcagaag cgttcgtgtg gcaggccgac
      601 ccgccaagct tcttgccggt gcggcaggtg aaccgcgtgc cgctccagct cctgaaaggc
      661 atcgagcacc acgccaatct gctgctggaa aacacggaac ggttcgcccg cggacttcct
      721 gcgaacaacg ccctgctttg gggcgcccgc ggcatgggga agtcatccct agtgaaggcc
      781 gctcatgcgg aggtgcgccg gcggctaggg ctcgggaagg atgcaaagcc ggacctgaag
      841 ctcgtggaaa tccatcgcga ggacatcagc tctctcccca tgtgcctcgc gttgatgcgc
      901 gagaaccacc atcgcttcat cgtgttctgc gacgaccttt cgttcgatcg cgatgacacg
      961 agctacaaat cgctgaaggc cgttctggag ggcggcatcg aggggcggcc ggagaatgtc
     1021 atcttctacg ccacctccaa ccgccggcat ctgatgccgc gcgatatgat ggagaacgag
     1081 cgctcgacgg ccatcaatcc ttccgaggcg gtcgaggaaa aagtctccct gtccgaccgc
     1141 ttcggcctgt ggctcgggtt ccatcacgga tcgcaggatc agtatttgga aatggtccgc
     1201 ggctacgcct cctatcaccg gctcgagatc gccgacgacg agctggttcg cgaggccctc
     1261 gaatggtcca tgacccgcgg cgggcgctcc ggccgcgtgg cgtggcagtt catccagcat
     1321 ctcgccggcc ggttggggca aaagctcaga acggagtgac taacacaagg gcccgcttca
     1381 acggcgggtc ctacttggtg agatcaagga ccacccgacg tacgctcaga tctcctggct
     1441 ctggctggat cgagaagccc agctcgctgc acatcttgag catcgtcgta ttctcggcga
     1501 gcaccgagcc gaatagctcc tcaaggcctt ctgagcgggc gtaggcgata aggtgctgca
     1561 tcagctgcca cccaagcccc cggcctttga gatcggacct tacgaggatc gcatattccc
     1621 cgcgcgtgta atccggatca gctacgaaac gtgaaactcc gagcaagtgg cccgtgtcct
     1681 tcgagatcgc gacgaacgcc atctcccgcg cgtaatcgat ctgcgtcagc cgggccagga
     1741 atttccggga cagatcgacg ccggcgctca agaagcgcag ccggtgatcc tccttcgtga
     1801 catgctggaa gaactcgtcg tagagcggtt cgtcctcggg ccggatcggc ctaagccgga
     1861 cagcgccaat cccctccaac accagatcct tgatccactc cgagggatac ggcctgagcg
     1921 ccatcggcac ccgcgggttc acggcctcat cctcgacccg gacccgcgca tcgagcgcga
     1981 tcacaccgtt ttcgtcagcg agcaacggat tgatgtcgat ctcgcggatc tccgggtgcc
     2041 gcgcgacgag atagctgagc cgcactagcg cctccgcgat cttgtcgatc gcggcggccg
     2101 gccggtcgcg gtagccctgc agcaagcgcc agatccgcgt ccgccgcatg agatcctcgg
     2161 cgaggttcat gtcgagtggc ggcagggcct gggccgtatc c
//
LOCUS       DASUMG010000389         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1094363, whole genome shotgun sequence.
ACCESSION   DASUMG010000389 DASUMG010000000
VERSION     DASUMG010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094363"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="VFX46_06400"
     CDS             <1..175
                     /locus_tag="VFX46_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX5600244.1"
                     /translation="DTLEELMAIMTAGRFRHIPVVEDGALVGIVSIGDVVKHHIAEVE
                     MEASALRTYLLAG"
     gene            782..>2066
                     /locus_tag="VFX46_06405"
     CDS             782..>2066
                     /locus_tag="VFX46_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX5600245.1"
                     /translation="MIGGLKKTTSRFAIAAAASVIATSAMAADLGGNCCADLEERIAE
                     LEATTARKGNRRVSLTVYGQVNEGVLWWDDGEESNAYVVTNENSRTRFGFRGDARINP
                     EWSAGFLLEIGVRIANSGHVNQFVDDNVASIDALDLRHSAWYIESNRLGRVWVGHTNM
                     ATEGITEINLSNAAVVGAVDVNNFAGGFFVRLNNGRLLAAGSGDSPVLTGLRWRDLTS
                     QFFGQANPGDGTRANLVRYVSPTFSGFTFSASWGEDDFWDVALRYANEFNGVRVAGGI
                     GYQKWTDGNSFDLVRGVGEEDVIAGVLTGSQGEPGCADLHRLTNPVDAAIAGAEGHDV
                     DCHALGLSGSVMHVPTGLYLFGAYGFVKDENRRLLFALGTDGAVPDADDRDEFWYVQA
                     GIEKNFTGLGNTTIYGEFYKGDAGAGLNLGAVRNIG"
BASE COUNT          391 a          594 c          667 g          414 t
ORIGIN      
        1 cgatacgctc gaggagctca tggccatcat gactgcggga cgcttccgcc acattcccgt
       61 tgtggaggat ggcgccctgg ttggcatcgt ctccattggc gacgtggtca agcaccacat
      121 cgccgaggtc gagatggagg cgagcgcact gcgaacctac ctcctggccg ggtaaccaat
      181 ccctttaggc cggcctcagc cgaaaaggcg agctccctag gctgggcgcg gcggctgatt
      241 cgcatcgcga tcactctctt tcccttggct tcctgacagc ccatacacag atcccgcctt
      301 cgtttgcgcg tccgagaggc ccaatttgcg ccctcccgca gcgttgcagc tgcgcaattc
      361 gtgacaaatc agcaacgatt tcaaacgaaa tatgaataca actcaagcgc gagtttgctc
      421 gctgcgcaca actgcactaa agcaataatg cgaacaacct aagagggtgt tcgtcggcgg
      481 tcgcaaccat catcggccgc tgagtacgag tgtcgtgcgg cggagcctca tcgaggatcc
      541 accaaggcgg aaccgggacc gcggagcgcc cggaaccgcg ctgcagagag caggcccggc
      601 gggctggtta agccgcactc tgagttccag gcgttcagtt cggttcgcgt ctcccgagta
      661 ccgcgtccag tcggagtggc gtgcgcacac gcgcgtgcgt gcacctcgga ccgggcgcgc
      721 gagctgtcgc cgggcagcgc gtgctgcttt ctgcgaaacg gaacaacgag tggagatgga
      781 aatgattggg ggactgaaga agacgaccag tcgcttcgcc atcgccgccg cggctagcgt
      841 catcgcgact agtgccatgg cggccgatct cggcggcaat tgctgtgccg atcttgagga
      901 gcgcatcgct gagcttgagg ccaccaccgc ccgcaagggc aaccggcgcg tgtcactcac
      961 cgtctacggc caggtgaacg agggcgttct ttggtgggat gacggagaag agagcaacgc
     1021 ttacgtggtg acgaacgaga actcacgtac tcgtttcggt ttccgcggcg atgcgcggat
     1081 caatcctgag tggagcgcgg gcttcctgct cgagattggc gtgcgtatcg ccaacagcgg
     1141 ccacgtgaat cagttcgtcg atgacaacgt ggccagcatc gacgcgctcg atcttcgtca
     1201 cagcgcttgg tacatcgaat caaaccgcct cggccgcgtg tgggtcggcc atacgaacat
     1261 ggcgaccgag ggcatcacgg agatcaatct gtccaacgca gccgtcgttg gcgcggtgga
     1321 tgttaacaac ttcgctggtg gcttctttgt gcggttgaac aatggcaggc tcttggccgc
     1381 tgggtcgggc gattcgcccg tattgacggg cctgcggtgg cgggacctta cgtctcagtt
     1441 ctttgggcaa gcaaatccgg gcgacggcac gcgagccaac ttggtgcgct acgtttcgcc
     1501 taccttctcc gggtttacgt tttcggcctc gtggggagag gatgatttct gggatgtcgc
     1561 tctaaggtat gccaacgagt tcaacggtgt gcgtgttgcc ggcggcattg gctatcagaa
     1621 atggacagac ggcaattcct tcgacctcgt gcgtggggtg ggcgaggagg acgtcatcgc
     1681 cggggtctta acagggagcc agggggagcc cggctgtgct gatctccaca ggctcacgaa
     1741 cccggttgat gcggcgatcg ccggcgccga agggcacgac gtggattgtc acgcgctcgg
     1801 cttatcgggc agcgtcatgc atgttcctac cgggctgtac ctctttggtg cgtacgggtt
     1861 cgtaaaggac gagaataggc ggttgctctt tgctttggga acagatggag ctgttcccga
     1921 cgcggatgat cgcgacgagt tctggtatgt gcaggcgggc atcgagaaga atttcaccgg
     1981 cctgggcaac acaaccatct acggtgaatt ctacaaagga gacgctggtg ccggcttgaa
     2041 tctcggtgcc gttcgtaaca tcggcc
//
LOCUS       DASUMG010000390         3354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_292131, whole genome shotgun sequence.
ACCESSION   DASUMG010000390 DASUMG010000000
VERSION     DASUMG010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292131"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /gene="cpaB"
                     /locus_tag="VFX46_06410"
     CDS             complement(<1..526)
                     /gene="cpaB"
                     /locus_tag="VFX46_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEX5600246.1"
                     /translation="MLLIVLCASLIGLLASFLVYRVVSQVARANDDQSDRIVVAAANI
                     GLAETVTGQHVRLVPWPKAAVPPGAIRSLADAEGRVVRGSIVAGEPLMEAKLAPQLSG
                     KGGIMPMLVPEGQRGVTIKLDDATRDSGFVLPNSRVDVLVSMPKAPGANEKIAKVILQ
                     DVTVLAAGQTVEMKD"
     gene            complement(555..989)
                     /locus_tag="VFX46_06415"
     CDS             complement(555..989)
                     /locus_tag="VFX46_06415"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HEX5600247.1"
                     /translation="MRLMRRLGNQRGTSTLEFLVVLPTLLFIFLASLELSRAWLTSNI
                     ATNAAREGARVGVVTPPPDPSSPSFNNGPALTRVNEVLSAANLTAASVSVTCSATACP
                     PDSQVQATVVINFETVVPLFLPVLGTPGSPLVLTETVRMRRE"
     gene            complement(1059..1235)
                     /locus_tag="VFX46_06420"
     CDS             complement(1059..1235)
                     /locus_tag="VFX46_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600248.1"
                     /translation="MKRILGYLKDERGVETLEWIAVGALIVMLAVAVYPGTLKAGIDG
                     VITDITNKLTGIVT"
     gene            complement(1341..1598)
                     /locus_tag="VFX46_06425"
     CDS             complement(1341..1598)
                     /locus_tag="VFX46_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600249.1"
                     /translation="MSHAESLVVVKLDHKGGQAPAVFEALEQAGLLGEVSESELDLVE
                     PGAVGFRIPRDRMAAVVLALECNGFADVRAYEVQHDRDGGG"
     gene            2161..>3354
                     /locus_tag="VFX46_06430"
     CDS             2161..>3354
                     /locus_tag="VFX46_06430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX5600250.1"
                     /translation="MPIDVTTRHAGLDSTQADSTTTASLEMLEAMVEGAREGLCILSP
                     QAILLRANQAAGELLGFEPRQATGRPVHELGVDRDFDWSIGGKVAATRVAVSTIQTLR
                     NGRKVLVSGVPVVSGAGLSYVVVTIREITGMGQMMSRLRDAVELAEQSRSTLAITTGR
                     EPQLDEVVANSRPLAAVRDKALQYAAVDSTVLLLGETGAGKGLFARLIHQASARSGGP
                     FLEVNCGAIPEGLMEAELFGYAKGKIGLVELAHKGTLLLNEIGDLPLSLQVKLLRFLE
                     DGEVWAVGAVKPKRPDVRIIAATNRDLPGMIAEGRFRGDLFYRLDVLALQIPALRDHP
                     EDIPRLVEVALVRAEKRLRRRRGITPEAVEALSRYPFPGNVRELSNLAERLVVTATSE
                     MIDVAD"
BASE COUNT          536 a         1120 c         1100 g          598 t
ORIGIN      
        1 tgtccttcat ctcgacggtc tgaccggccg cgagcaccgt gacgtcctgc agaatcacct
       61 tggcgatctt ttcgttcgcg ccgggcgctt tgggcatgct caccagcacg tccacccggc
      121 tgttcggcag cacgaagccg ctgtcgcggg tggcatcgtc cagcttgatg gtgacgccgc
      181 gctggccttc gggcaccagc atcggcatga tgccgccctt gccggaaagc tgcggggcga
      241 gcttggcttc catcagcggc tccccggcca cgatggatcc gcgcaccacc cgtccctcgg
      301 cgtccgccag actgcggatc gcgccgggag gcaccgccgc cttcggccag gggacgagcc
      361 tcacgtgctg accggtgacg gtctcggcga gcccgatgtt ggccgccgcc accacgatgc
      421 ggtcggactg atcatcgttg gcccgggcca cctggctgac cacccggtag acgaggaagc
      481 tcgccagcag gccgatgaga gaggcgcaca gcacaatcag cagcaatcgc cgacgcatag
      541 tgtccctact ttccctattc gcgtcgcatc ctgaccgtct ccgtgagaac cagagggcta
      601 cctggcgtcc ccaggaccgg gaggaagagc ggcacgacag tctcgaaatt gatgaccacg
      661 gtcgcctgca cctgcgaatc gggcggacag gcggttgcgg agcacgtcac cgacacgctg
      721 gccgcggtga ggttcgccgc gctgaggacc tcgttcaccc gggtcaacgc cggcccgttg
      781 ttgaacgaag ggctgctcgg atccggagga ggtgtgacca cccccacccg cgcgccttcc
      841 cgggcggcgt tggtggcgat gttcgacgtg agccacgccc ggctcagctc gagagacgcg
      901 aggaagatga acagcagcgt cggcaggacc acgaggaact cgagcgtgga ggtgcctcgc
      961 tgattgccca gtcgtcgcat gagtctcatg gtcttgtgcc ttgtctgttt gtgccagctc
     1021 ccggggcccg tgagggcccc gggattgggc gtgggtccct acgtgacgat accggtcagc
     1081 ttgttggtga tgtccgtgat gacaccgtcg atcccggcct tgagggtgcc ggggtagacg
     1141 gcgaccgcga gcatcacgat cagcgcgccc accgcgatcc actccagcgt ctcgacgccg
     1201 cgctcgtcct tcaggtatcc gagaatccgc ttcattgcct gcctctctcc tgtgctcacc
     1261 accgctcggt ggtgaggccg agaagttcac tgcgcctgac tacggtctgc cgcgctaact
     1321 gccgccccgt ctcccccctc tcatccgccg ccgtcccgat cgtgctgcac ctcgtacgcc
     1381 ctcacgtccg cgaagccgtt gcattccaga gcgaggacca ccgcggccat tcgatcccgc
     1441 gggattcgga accccacggc tcccggctcg accaggtcca gctcgctctc ggagacttcg
     1501 ccgagcaggc cggcctgctc gagcgcctcg aacaccgccg gagcctgccc acccttgtgg
     1561 tctagcttca cgaccacgag cgactccgcg tggctcacgc ttgatccctt tcaacctctc
     1621 cgggaagcac cggagtgatg ttgctatctg tgtgactgcc ttctgccgtt gtcgctggcg
     1681 atgcgggcaa agtgcagagc gcgtgccatg cgctcggtgt cccgacccgt gaacatcaac
     1741 caggctatgt tggcggagag ttacgcgtga agtccggcat cacgttgccg cgcggtgact
     1801 cgatgcggaa acggattgcc acatgatggc gagcgatgag aagcaacgag ccgacgaggg
     1861 cgaacctcca acattctcgc cgggctccgc gtgcgcttac ggtgctatgc ggaatcgcat
     1921 atcgactttt ggttctcgcg agcgcggaat cattgcgcag gacagaagat ggacaacgcg
     1981 cccgtctcgt tatccagatt gggacaatct ttttcccttg ccggggcggg tccggactgc
     2041 gcttcgggcg attggtctta aatatcggtt tcttctcgca tgtcttctgc ggattgcatc
     2101 ctggcacttc tcctgctctc tactctcgcg acagaatctg ggtggaccca cgaggatgcc
     2161 gtgcctattg atgtgacgac gcgccacgcc gggctggatt cgacgcaagc cgactccacg
     2221 acgacggcca gcctcgagat gctcgaggcg atggtcgaag gcgcgcgcga aggcctgtgc
     2281 atcctgagcc cgcaagcgat cctgcttcga gcgaatcagg cagccggcga gctgctcggc
     2341 ttcgagccga ggcaggcgac gggccggccg gtccacgagc tcggggtgga tcgcgacttc
     2401 gactggtcga tcggcgggaa agtcgcggcg acgcgggtcg ccgtgtcgac gattcagact
     2461 ctccgcaacg gacggaaggt attggtctcc ggcgtccccg tcgtgtcggg tgccgggctc
     2521 tcctacgtcg tcgtgaccat ccgggagatc accggcatgg gccagatgat gagccggctg
     2581 cgcgatgccg tcgagctggc cgagcagtca cgctccacgc tggccatcac caccggccgc
     2641 gagccgcagc tggacgaggt cgtcgcgaac agtcggccgc tcgccgcggt gcgggacaag
     2701 gccctgcagt atgcggcggt cgactcgacg gtgctgttgc tcggggagac cggagccggg
     2761 aaggggttgt tcgcccggct catccaccag gcctccgccc ggagcggagg cccctttctc
     2821 gaggtgaact gcggcgcgat cccggaaggc ttgatggagg ccgagctgtt cggctacgcc
     2881 aagggcaaga tcggcctcgt cgagctggcc cacaagggga ccctgctgct gaacgagatc
     2941 ggtgatctcc cgctgagcct gcaggtgaag ctgctgcggt tcctggagga cggcgaggta
     3001 tgggcagtag gagccgtgaa gcccaagcgt ccggacgtgc ggatcatcgc cgccaccaac
     3061 cgcgatctgc caggcatgat cgcggagggc cgcttccgcg gggatctctt ctatcgcctc
     3121 gacgtgttgg cgctccagat tccagctctc cgcgaccatc ccgaggacat ccctcgcctg
     3181 gtcgaggtgg ccctggtgcg ggccgagaag cgactccgga ggcgccgggg catcacgccc
     3241 gaggcggtcg aggcgctctc gcgctatccc tttcccggga acgtgcgcga gctgtccaac
     3301 ctggccgagc ggctcgtggt gacggccacc tccgagatga tcgacgtggc cgac
//
LOCUS       DASUMG010000391         3218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_773456, whole genome shotgun sequence.
ACCESSION   DASUMG010000391 DASUMG010000000
VERSION     DASUMG010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773456"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFX46_06435"
     CDS             <1..387
                     /locus_tag="VFX46_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX5600251.1"
                     /translation="RYVAHRFALVGDAAHGVHPIAGQGLNLAFRDIAALTELVADAVR
                     IGLTPGDASVLERYEQWRRFDSGVSAATFDALNRLFSNDITLLRSVREAGLALVDRLP
                     ALKRMFVSEAAGLSGEVPRLLRGELA"
     gene            complement(450..896)
                     /locus_tag="VFX46_06440"
     CDS             complement(450..896)
                     /locus_tag="VFX46_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006196483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX5600252.1"
                     /translation="MYKHILIATDGSELADKGVEHGLALASALGSKVSVLTVSAPLSS
                     EAIQAALAGGIEDPVGRYDQQIDTLMKERFAAIGKRAEKHGVVVEYVHEIDESPAEAI
                     VRFAKLRNCDLIVMASHGRRGAKRLLLGSQTAEVVTTTTIPVLVVR"
     gene            complement(1010..2077)
                     /gene="hemH"
                     /locus_tag="VFX46_06445"
     CDS             complement(1010..2077)
                     /gene="hemH"
                     /locus_tag="VFX46_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX5600253.1"
                     /translation="MNEVPKANTIGMDQAERSVLPADHPPVRHGRIGVLLLNLGTPDA
                     TDYWSMRRYLKEFLSDRRVIETPRIIWWPVLNLIILTKRPRAKGRDYDTIWNKEKDEG
                     PLKTITRDQAEKLAAALDNPRIVVDWAMRYANPTTESRIKALMEQGCDRILLVPLYPQ
                     YAAATTATACDQAFRALMKMRWQPYVRVAPDWHDDPVYIEALARSMEAHLAQIDFRPE
                     AVIATFHGMPEKYLHQGDPYYCQCEKTSRLLRERLGWPKESWHTTYQSRFGNDVWLQP
                     YTDETVKRLAQSGVKKLLLIAPGFTADCLETLEELEVENREIFMENGGEKFTYVPALN
                     ASPEGMRVIEHVVRRELMGWV"
     gene            complement(2258..2629)
                     /locus_tag="VFX46_06450"
     CDS             complement(2258..2629)
                     /locus_tag="VFX46_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003551292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HEX5600254.1"
                     /translation="MSNFVEKLSNRLPDAGNFLLGLWLVASPWVLGYAGETTAAWNTY
                     IVGVVIAALAALAFFAQQKWEHWVSLAIGAWLIVSPWVLAYSALQAAFWNQIVTGIIV
                     CALAIWAAASESGFGGLEARS"
     gene            complement(2855..>3218)
                     /locus_tag="VFX46_06455"
     CDS             complement(2855..>3218)
                     /locus_tag="VFX46_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX5600255.1"
                     /translation="GNIGHDAGLSLRPAVDIAEDDKELVISAEIPGLDEKDLDVSLAG
                     DLLTIKGEKKAEHEERNGGAYYVERRYGSFSRSVRLPFQVDDQDVEAKYEKGVLTIRI
                     PKPIEMQKAVRRIPINAS"
BASE COUNT          613 a         1015 c          962 g          628 t
ORIGIN      
        1 cgatacgtgg cgcaccgctt tgcgcttgtt ggcgacgctg cccacggtgt tcatcccatc
       61 gctggacagg gccttaactt ggctttccgg gatatcgcgg ccctgacgga attggtggcc
      121 gacgcagtgc ggatcggcct gacaccgggc gatgccagcg tgttggaacg ttacgagcag
      181 tggcgccgct tcgactctgg tgtatccgcg gcgacgttcg acgccctcaa ccggctattc
      241 tcaaatgaca taacgctatt gcgctctgtc cgggaggctg ggctggcact cgtcgaccgc
      301 cttccagccc tgaagaggat gttcgtctcc gaagcggccg gtctctctgg cgaggtcccc
      361 cgactgctcc gtggcgagct cgcataagcg gttccggatt ggcctaagcg gagcgaccgt
      421 ccgagttaca tcgcgacaga gatttgccgc taacggacca cgaggaccgg gatagtggtc
      481 gtcgttacca cctcagcagt ttggctgccc agaaggaggc gcttcgcccc tcgccggccg
      541 tgcgaggcca tcacgatgag atcgcagttc cgcagcttgg cgaacctgac gatcgcttct
      601 gcgggcgatt cgtcaatttc gtggacatac tcgaccacga cgccatgctt ttcggcgcgc
      661 tttccgatcg cggcaaatcg ctccttcata agagtgtcga tctgctggtc gtagcggccg
      721 acggggtcct cgatgccgcc agcaagtgcg gcttggatcg cctccgacga cagcggtgcc
      781 gaaacggtaa ggaccgacac cttggagccg agcgcgcttg cgagcgcgag cccatgctca
      841 acacccttgt ccgcaagctc ggagccatcg gtcgcaatca ggatgtgctt gtacatcggg
      901 gacctccgtc tgaatcgcgc ccacaagaaa tatgggcacg gtcggagggt gacggggtcc
      961 gcctcgtctg tccatgtttc agatcaattc ggcagtaaca ggccaggcgt cacacccagc
     1021 ccatcaactc ccgccgtacc acgtgctcga tgacacgcat tccctcaggg ctggcgttga
     1081 gcgcgggcac gtaggtgaac ttctcgccgc cgttttccat gaaaatctcg cggttctcga
     1141 cctcgagctc ctccagggtc tcaaggcagt ccgcggtgaa tcccggggcg atgagcagca
     1201 gcttcttgac gcccgattgg gccagtcgct tcacagtctc gtccgtatag ggctgcagcc
     1261 agacgtcgtt gccaaagcgt gactggtagg tcgtgtgcca gctctccttc ggccagccga
     1321 gccgctcccg caacaagcgg gatgtcttct cgcactggca gtaatacgga tcaccctgat
     1381 ggagatactt ctccggcatg ccgtggaacg tggcgataac ggcctcgggc ctaaaatcga
     1441 tctgcgccaa gtgcgcttcc atcgagcgcg ccaatgcctc gatatagacc gggtcgtcgt
     1501 gccaatccgg cgccacgcgc acataaggct gccagcgcat cttcatgagc gcgcggaacg
     1561 cctgatcgca cgctgtcgcc gtggttgcgg ctgcatattg cggataaagc ggcacaagca
     1621 gaatgcggtc gcagccctgc tccatcagtg ccttgatacg gctctcggtc gtgggattcg
     1681 cataacgcat cgcccagtct acaacgatgc gcgggttgtc gagagctgcg gcaagctttt
     1741 ccgcttggtc ccgggtgatg gtcttgagcg gaccctcgtc cttctccttg ttccaaatcg
     1801 tgtcgtagtc gcggcccttt gcccgggggc gcttggtcag gatgatgagg ttcaggaccg
     1861 gccaccagat gatgcgcggc gtctcgatca cccggcggtc cgaaaggaat tccttgagat
     1921 agcgccgcat cgaccagtag tcagtggcat ccggtgtgcc gaggttgagc agcagcacgc
     1981 caatgcggcc atgccgaacg ggcggatgat cggcgggcag caccgacctt tccgcctggt
     2041 ccataccaat cgtgttcgct ttgggcactt cgttcatcgt gcgggagcct ccacgaagcc
     2101 gtgaaaaagt gctctcgccc ccttctcggc caaaaaacgc cccggcgcca gcctgcctca
     2161 tgacgcaggg gcgtcagggc tcgtcgatgg ttttcgaagc aagctgaaaa gcgggagaat
     2221 gccggagttc ggcacacccc cacttacgag ccagacatca gctccgcgcc tcgaggccgc
     2281 cgaagccgga ctcgctggcc gccgcccaga tcgcaagggc gcaaacgatg atgccggtta
     2341 cgatctggtt ccagaaggca gcctgtaggg cgctataggc cagcacccaa ggcgacacaa
     2401 tcagccacgc gccaatggcc aagctgaccc agtgctccca cttctgctgc gcaaaaaacg
     2461 caagcgcagc cagggcagcg attaccacgc cgacgatata ggtattccac gctgcggtgg
     2521 tctcgccggc gtatcccaac acccaaggcg acgctacaag ccacagcccc aaaaggaagt
     2581 tgccggcgtc cggcaatcga ttggagagtt tttcgacgaa gttgctcatg tcacgtttct
     2641 cctctttgcg cttcagcgag atgatccgtg cggcgacgga gtcatccgcc gccgcgcggg
     2701 gatgagctca cggcatgaga acagcgacga tgaacaccag gctcaggagc agcatgttga
     2761 gcgcccagag cctggaaaga gtacgcctct caacacgcat tcggacctcc ttctcaacac
     2821 cgccagtgtg gagatgtcag tccgattagc ggagtcagga cgcgttgatc ggaattcggc
     2881 gcaccgcctt ctgcatctcg atgggcttcg gaatgcgaat ggtcagtacg cccttttcat
     2941 atttggcctc gacgtcctgg tcgtccacct ggaacggcag acgcacggat cgagagaagg
     3001 agccatagcg gcgctccaca tagtaagccc cgccgtttct ctcctcgtgc tcggccttct
     3061 tctcaccttt gatcgtgagg agatcgccgg cgagcgagac atcgagatcc ttctcatcga
     3121 gaccaggaat ctcggccgag atgacgagct ccttatcgtc ctcggcaata tcgacagccg
     3181 ggcgcaggct taggccagca tcatggccga tgttgccg
//
LOCUS       DASUMG010000392         2778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_773607, whole genome shotgun sequence.
ACCESSION   DASUMG010000392 DASUMG010000000
VERSION     DASUMG010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773607"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..83
                     /locus_tag="VFX46_06460"
     CDS             <1..83
                     /locus_tag="VFX46_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX5600256.1"
                     /translation="RSGVGFRLGANVGYLKYTRTPTWNPF"
     gene            217..1719
                     /locus_tag="VFX46_06465"
     CDS             217..1719
                     /locus_tag="VFX46_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600257.1"
                     /translation="MITIESTMLVALGFLLATLIAVLLAPAYRARAMRLTYKHMRRVM
                     PLTESELLLDRDRLRAHYAVRIHELETKLEKLRLAAARQQVEVNRRDATISALESELA
                     RIRAELEENVNARRVLEQTVMDRLPAVEQRLAETRKLLEQRDRELATITADTSKNARA
                     LDEVMQINAQQRAEIERLNAVIATRGLRTNRGSRDPRFENELALRSELEALRAKTREQ
                     ESLISRLQTLVTMPSQAGQAGRANGGGSSTEQEAELDRLRRDLVEAESALKSIAEQSD
                     GKEVADETMRARLQSLENLVADQTAKIKRLEAALAVYEKGEGDGRAISLKDSKIAMKA
                     RIASLQAEADERAATIEKLRAELAAANERLALQAAQFRDEVRRLRSGPQPTAPQKDEP
                     KAAARRSLAQRITDVNPALAASLRAGSYGAARRKAAAAEPADEQAPVNDAQEQAPLPQ
                     LNGPSAGPVAAKADGKENGKEAAELNAAASESDAQPKPRLLDRLAGISKQ"
     gene            1847..2260
                     /locus_tag="VFX46_06470"
     CDS             1847..2260
                     /locus_tag="VFX46_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600258.1"
                     /translation="MSKFVSELSKAKAPFATHPSTLQHQPRPRLAERLAALAPLARAP
                     LIADVDRDEPDGLSRLPHTTRSRSYQPNALVPLEPPQLPLTSEPPMLSPVGVAPLHLD
                     FDDDARAELRLKWLGFFVGLAISAAIGVGLYVALA"
     gene            2372..>2778
                     /locus_tag="VFX46_06475"
     CDS             2372..>2778
                     /locus_tag="VFX46_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5600259.1"
                     /translation="MLQFAENLNRLGTETAFEVLARAAELQRQGRDIINLGIGQPDFE
                     TPPHIVEAAVKALRDGHHGYTPAHGILPLREAVAADRKRRHNVEVNPDNIVIMPGGKP
                     TMFFSILMFGEPGTEIMYPDPGFPIYRSMIEYTG"
BASE COUNT          536 a          931 c          849 g          462 t
ORIGIN      
        1 ttcgttcagg agttggcttt aggctaggtg cgaacgtcgg ctacttgaag tatacccgga
       61 ctccgacttg gaatcccttc tgatcaggcg ttagccgatc cgtgaagggg aacggatcgg
      121 gacgaacgag attgcgttgt aacttcatcg ggggccggtc gtcagattcg gcccggtcca
      181 ctgtgtgctg ttaggattgc gttcggggat gcctcagtga tcacgatcga gtcgaccatg
      241 ctcgtggcgc tggggttcct gcttgcgacc ctcattgccg ttcttctcgc cccggcctac
      301 cgggcacgcg ccatgcgctt gacctacaag cacatgcgcc gcgtgatgcc gctcactgaa
      361 agcgagctgc tgctcgatag agacaggctc cgggcccatt acgccgttcg catccacgag
      421 cttgaaacga agctggaaaa gctgcgcctg gcggccgccc gccagcaggt ggaggtcaac
      481 aggcgcgacg cgactatctc cgcactggaa tccgagctgg ctaggatccg ggccgagctg
      541 gaagagaacg tcaacgcacg ccgcgtgctc gagcagaccg tcatggatcg tctgcctgcc
      601 gtcgagcagc ggcttgccga aacccgcaag ctgctcgagc agcgggaccg cgagctggcc
      661 acgatcacgg ccgatacgag caagaatgcc cgcgcgctcg acgaggtcat gcagatcaat
      721 gcccagcaaa gggctgagat cgagcgcctc aacgcagtca tagccacgcg cggcctccgc
      781 accaaccgcg ggtcgaggga tccgcgcttc gaaaacgagc tggcgctcag gtcggagctg
      841 gaggccctgc gcgccaagac ccgcgagcaa gagtcgctga tttcgcgcct gcagactctc
      901 gtaaccatgc cctcgcaggc cgggcaggct ggtcgtgcga atggtggcgg ctccagcacc
      961 gagcaggagg ccgagctcga tcgcctgcgc cgcgacctcg tagaagccga gagcgccctc
     1021 aagtccatcg ccgagcaatc cgatggcaag gaggttgctg atgagaccat gagggcgcgg
     1081 ttgcagtcgc tcgaaaacct cgtcgccgac caaacggcga agatcaaacg gctcgaggca
     1141 gctctcgcgg tgtacgagaa gggcgaaggc gatggccgcg ccatctcgtt gaaggacagc
     1201 aagatcgcga tgaaggctcg cattgcgtcc cttcaggccg aggcggatga gcgcgccgcc
     1261 accatcgaga agctgcgcgc cgagctcgcc gcggccaacg agcggctcgc cttgcaggcc
     1321 gcccagttca gggacgaggt ccgccggctg cgctccggtc ctcagcccac agcgccgcag
     1381 aaggacgagc cgaaggccgc agcccggcgc tcgctcgccc agcgcatcac cgacgtcaac
     1441 cccgcactgg ccgcatcgct cagggcggga tcttacggcg ccgcaaggcg caaagcggcg
     1501 gcggccgaac ccgccgacga acaagccccc gtcaacgacg cccaggaaca agctcccttg
     1561 ccgcagttga acggcccctc ggccggcccc gtggccgcaa aggctgacgg caaggaaaac
     1621 ggcaaagaag ccgccgaatt gaacgctgca gcgtccgaat ccgacgccca accgaagccg
     1681 cgattgctcg accggctcgc cggcatcagc aaacagtgag ctgacgacta tccaccggct
     1741 tgacctttga tccacagccg agtgtttttc gctttccgcg cgagcgtttg gcagttaaat
     1801 caaccttaag gggaggagaa gcccgcaatg cacagtgtag aagagcatgt ccaagttcgt
     1861 ctcggaactg agtaaagcga aggctccctt tgcgacccac ccatcgaccc tccagcacca
     1921 gccccggccg cgccttgccg aacggcttgc tgctcttgcg ccgctggccc gtgcgcctct
     1981 gatcgcagac gtcgatcgcg atgagccgga cggcttgagc cgtttgcccc acacgacgcg
     2041 atcgcgatct tatcagccga atgctctggt tccgttggag ccgccccagc tccctctcac
     2101 cagcgagccg ccgatgctct ctccggtcgg cgttgctccc cttcacctgg atttcgatga
     2161 tgatgcacgc gcagaattgc ggctgaagtg gctaggcttc ttcgtcggcc ttgcgatctc
     2221 cgcggctata ggcgtcggtc tctacgtcgc gctggcctga gccagcgctc ctcattgcaa
     2281 gttgtgcaac cctccttttg cgggacacca cttccccttc tactcgcctc gtgcaagatt
     2341 ccccgcgagt agcatattga tggggagcga catgctgcag tttgcagaga atcttaaccg
     2401 cctaggtaca gaaacggcgt tcgaagtgct tgcgcgcgcc gccgagctgc agcgacaagg
     2461 ccgcgacatc atcaacctcg gcatcggcca gccggacttc gagacaccgc cgcacatcgt
     2521 cgaggctgcc gtgaaggcgc tgcgggacgg gcaccacggc tacacgccgg cgcacggcat
     2581 tctgcccctc cgcgaagccg tcgccgcgga ccgcaagcga cgccacaatg tcgaggtcaa
     2641 cccggacaac atcgtcatca tgcccggcgg caagccgacg atgttcttct ccattctcat
     2701 gttcggcgag ccgggcaccg agatcatgta ccccgatccg ggcttcccca tttaccggtc
     2761 gatgatcgag tacaccgg
//
LOCUS       DASUMG010000393         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_372746, whole genome shotgun sequence.
ACCESSION   DASUMG010000393 DASUMG010000000
VERSION     DASUMG010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372746"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..942
                     /locus_tag="VFX46_06480"
     CDS             199..942
                     /locus_tag="VFX46_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HEX5600260.1"
                     /translation="MAMKRSIRSPVLFAAILSVLLTVPAAAQRSAPQSQHEAGVFDYY
                     TLVLSWSPTYCASMRSPDDDPQCRRTARPYAFVLHGLWPQYERGFPEYCPTRERPFVP
                     QRVIDQMLDIMPSPRLVIHEYRRHGTCSGLDPAGYFELSRRLYEKINIPRRFRLPPAP
                     FFISPQQVVDEFIAANPGLKVEMITVDCRGSGNRLREVRICFSREGQFRPCGSNERRA
                     FCRASRMYVPPVRIGGGAGTTKPQGERAL"
     gene            1078..1473
                     /locus_tag="VFX46_06485"
     CDS             1078..1473
                     /locus_tag="VFX46_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600261.1"
                     /translation="MAKSRHAHPALDATARPSHMRGARSRAFIWAAVGFVAGTLFWSI
                     TGLDPNPRVLARTFAPADTSSTRTDTTKPADRVPTSLSAAGNIRHDCATLVLDRALKQ
                     TRLHPCTTDDPPLRFATSAGRQDRLAVVE"
     gene            complement(1589..>2107)
                     /locus_tag="VFX46_06490"
     CDS             complement(1589..>2107)
                     /locus_tag="VFX46_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600262.1"
                     /translation="YDDELGEKHVFVSPPVSGWTFVVGLSLPYPVGRSFVDKCTPMLL
                     RLGAEFIEVQYFFSYPPIDFFAWARIVHGRLIRAFAIGDEGTIWNRGKPTRDEKRLGL
                     KLFELRGVRERTGDAGGELILYPTEEHVMRLAGYWSLDPTRIDAAPASTASGYICLAP
                     AAWRPERLRRTA"
BASE COUNT          389 a          697 c          606 g          415 t
ORIGIN      
        1 ctatcgtcac cgcgttcaag acacaccttg gcatggacag cctgctcatc ggtttcggca
       61 ccgatgacga ctgcattcac tcacccaacg agaagtacaa tctgacgagc ttccgcaagg
      121 gcgcgcggag ctgggcccgc attctccagc gactcgcgcg cgtttgatca gttcgaccac
      181 aaacgatcga gactagatat ggcgatgaaa cgttccatcc ggtcgccggt gctcttcgcg
      241 gcaatcctct ccgtgctgct caccgtcccg gctgcggccc agcggagcgc cccgcaaagc
      301 caacatgagg ccggggtttt cgactactac acgctcgtat tgtcttggag cccgacgtac
      361 tgcgcgagca tgcgctcacc agacgatgat ccgcagtgcc gtcgcacagc ccggccttat
      421 gcctttgtgc tccatggctt gtggccgcaa tacgagcgcg ggtttccaga gtactgcccg
      481 acacgggagc gtccgttcgt tccccagcgc gtgatcgacc agatgctcga catcatgccg
      541 agcccgcggc tggtcattca cgagtatcgc aggcatggca cgtgctcggg gctcgatcca
      601 gccggttatt ttgagctttc gcggcggctt tatgaaaaga tcaatattcc gaggagattc
      661 cgcttgcctc ctgcgccgtt cttcatcagt ccacagcagg ttgttgacga gttcatcgcc
      721 gccaatcctg gcctgaaggt cgaaatgata accgtcgact gtcgcggctc cggtaaccgg
      781 ctgcgtgagg tgcgaatctg cttttcgcgc gaaggacaat tccggccttg tggtagcaac
      841 gagcgccgcg ccttctgccg ggccagccgc atgtatgttc ctcccgtgcg gatcggcggt
      901 ggcgctggca ccaccaagcc gcaaggggag cgtgcacttt agtcaatacg cacgttgaga
      961 tttccgtgga gaactcgcga tgctgccagc ctgtgcggct ggcgcctgtt gcagtgtggt
     1021 ggccgttcgg catcatggta gccggaacgc aacagattcg cgctcgcgag ggattccatg
     1081 gcgaagtcgc ggcacgctca tccggccttg gatgccaccg cccgaccgtc acacatgcgc
     1141 ggtgcacgga gcagggcctt catctgggct gctgtcggtt ttgttgctgg aacgctgttc
     1201 tggagcatca cgggcttaga cccaaacccg cgagtattgg cacgcacctt tgcgccggcg
     1261 gacacctctt cgacccgcac tgacaccact aagcctgcgg acagagtgcc gacgtcgctt
     1321 tcagctgccg gcaatattcg gcacgactgc gcgactctcg tgctcgatcg agcgctcaag
     1381 cagacaaggc tgcatccctg caccactgac gatccgccct tgagattcgc gacaagcgct
     1441 ggtcgtcagg atcgcctcgc cgtcgtggaa taagccgcta cgccagctct ctcacctcac
     1501 tcagcgcgtc tacaaaattc ccccaagcaa caggcttggc ggcttcaccg ctgcgaccca
     1561 agctaagcgg ctgccttggg gtgggttgtc aggccgtcct gcgcagacgt tccggtcgcc
     1621 aggctgccgg cgcaaggcag atgtaccccg aagccgttga tgccggagcc gcatcgatcc
     1681 gagttggatc cagactccag tatccggcga gccgcatgac gtgctcctcc gtcgggtaga
     1741 ggatcagctc accccctgcg tcgcccgtcc gctcacgaac gccgcgcagc tcgaacagct
     1801 tgagacccag cctcttctcg tcgcgtgtcg gcttgcccct gttccagatc gtcccttcat
     1861 cgccaatcgc aaacgcacga atgagccttc cgtggacgat ccgcgcccaa gcgaagaagt
     1921 cgatgggcgg atagctgaag aaatattgca cctcgatgaa ttccgctccc agcctcagga
     1981 gcatcggcgt gcatttgtcg acgaagctcc gcccgaccgg ataaggcagc gacagtccga
     2041 ccacgaacgt ccagccgctc accggcggcg acacgaacac gtgcttctcg cccagctcgt
     2101 cgtcgta
//
LOCUS       DASUMG010000394         3318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_212388, whole genome shotgun sequence.
ACCESSION   DASUMG010000394 DASUMG010000000
VERSION     DASUMG010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212388"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="ruvA"
                     /locus_tag="VFX46_06495"
     CDS             <1..535
                     /gene="ruvA"
                     /locus_tag="VFX46_06495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5600263.1"
                     /translation="RTLRSLKLGQEVTLSIETYVREDAIRLFGFTTEVERAWFRTLQS
                     VQGVGSKVALAILGTLSTQDLANAIALGNRAAIEQAPGVGRKLAQRIVTELKEKAPQL
                     SIGVHAPTAEMRANGAGGPVLLQEGNVSAEAISALTNLGYPPQQAAAAIAAAMSDLGD
                     AADLAQLIKRGLRELAR"
     gene            558..989
                     /locus_tag="VFX46_06500"
     CDS             558..989
                     /locus_tag="VFX46_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600264.1"
                     /translation="MSNAQRALWTFLFYTLVGPFIGSLLLSVAIPLALVAGFLPDLAD
                     LESGGRISFTGWAALYAYVWGAPTAALAALGLLPFVFRGGTFSWIAAAVAGVIAFGVT
                     TIFAPLPVPGTAPYLAFLAGVVSLICWAVLSKMGVLAGADE"
     gene            1156..2253
                     /gene="ruvB"
                     /locus_tag="VFX46_06505"
     CDS             1156..2253
                     /gene="ruvB"
                     /locus_tag="VFX46_06505"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5600265.1"
                     /translation="MTDRLVTGAKRETDAFEATIRPQSLDEFIGQEQARANLRVFIQA
                     ARARGDALDHVLFAGPPGLGKTTLAQILAKELGVGFRATSGPVISKAGDLAALLTNLE
                     ERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPAARSVRIDLPKFTLVGATTR
                     AGLLTTPLRDRFGIPIRLNFYTVEELEQVVTRGARVLGANINAEGAREIARRSRGTPR
                     IAGRLLRRVWDFAVVANAKVIDAAVADKALRALEVDRAGLDALDHRYLTCIATNYEGG
                     PVGIETIAAALSEPRDALEEIVEPYLLQQGFIGRTPRGRIVTLKAYRHLGLAGPARSS
                     APQMGLFDPDTDDGADSETGAAGKDRNSRLR"
     gene            2314..3246
                     /locus_tag="VFX46_06510"
     CDS             2314..3246
                     /locus_tag="VFX46_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5600266.1"
                     /translation="MGRSDSDGGPAGSTAAADYAQTNNTAGIIAMLAAMASFACGDSL
                     MKLSSTDLPTGELVWLRATFMSAAGLTYAVLSGAVRSAWTLLARPMLLRAIGDTGGAL
                     AYQSALARMAFADLVAINQINPLTVTAASAIFLGERVGWRRWTAAAVGLCGVLLIIRP
                     GTSAFTWWSIVAVVAVLFATLRDVSTKSIDRAVPAVLVMWFSSSAVAFGSLTMLFFES
                     WVWPEPAVLLQVAGASAFSLTGHLCIITAVRKGELSAVAPFRYSIMIWALLLGLVIWG
                     ELPDGLAMLGMAIVTGAGIYTFRREMVARRKARP"
BASE COUNT          529 a         1039 c         1081 g          669 t
ORIGIN      
        1 tcgcacgctg cggagcctca agctgggcca ggaggttacg ctgtcgatcg agacctacgt
       61 gcgcgaggat gccatccgcc tgttcggctt caccaccgag gtggagcggg catggttccg
      121 cacgttgcag tccgtgcaag gcgtgggttc gaaagtggcg ctcgccattc tcggtacgct
      181 gtccacgcag gatctcgcca acgccatcgc gctggggaac cgcgcagcaa tcgagcaagc
      241 tcccggcgtt ggaaggaagc tggcgcagcg gattgtcacc gaattgaagg agaaggcgcc
      301 gcagctgtcc atcggcgttc atgcgccgac tgccgagatg cgggcaaatg gcgccggcgg
      361 acccgtgctg ctgcaggagg gcaatgtttc ggcggaagcc atatcggccc tgaccaatct
      421 cggctatccg cctcagcagg ccgcggccgc tatagccgca gctatgagcg atcttggaga
      481 tgcggcagat ctcgcccagc tgatcaagcg gggcttgcgg gagctggcgc gatgacggac
      541 ctgatgtcat ggatggcatg agcaacgcgc agcgcgcgct ctggactttt ctcttctaca
      601 cgctcgtcgg gccgttcatt ggctcgctac tgctcagcgt agcgatccca ttggcgctgg
      661 tggccggttt tctgcctgat cttgcggacc tcgagtcggg tggacggatt tcctttaccg
      721 gatgggctgc tctctacgcg tacgtctggg gtgcgccgac cgcagcgctc gccgcgctcg
      781 gtctcctgcc gttcgtgttt cgcggaggca cgttcagctg gatcgccgca gcggttgcgg
      841 gcgtgatcgc gttcggggtc accacgatct ttgcacccct gccggtgccc ggtacggctc
      901 cgtacctcgc ctttctcgcg ggcgttgtct ccctcatctg ctgggccgtt ctttccaaga
      961 tgggggtgct tgcgggagct gatgagtaat tctcgcgtcc aagagcagct acgtaagctg
     1021 agctgaggcg attgccgtcg tcggattcct tcgtggtcac taacatcctg atcaacagcc
     1081 tgccggacga cgtcccacga attgtgatag aggttcggtg agcacgaatc acgaacagga
     1141 gcgggggcag gtgcaatgac ggaccgcctc gtcacaggcg cgaagcgcga aacggatgcc
     1201 ttcgaggcga cgatccgccc gcaatctctc gatgagttca tcggccagga gcaggcacga
     1261 gcaaacctga gagtgttcat ccaagctgcg cgggcgcgcg gcgatgcact cgatcacgtc
     1321 ctgttcgccg ggccgccggg gctcggcaag accacactcg cgcagatcct cgcgaaggaa
     1381 ctcggcgtcg gctttcgcgc cacctccgga cccgtcatat ccaaggcggg cgatctggcg
     1441 gccctgctga ccaatctcga agaacgcgac gttctcttca tcgacgagat ccatcgtctc
     1501 aatccggcgg tcgaggaaat cctctatccc gccatggagg attttcagct cgatctgatc
     1561 atcggcgagg gccccgccgc tcgctccgtt cgcatcgact tgccgaagtt cactctggtg
     1621 ggcgctacca ctcgcgcggg cctgctcacg acgccgctgc gggaccgctt cggaataccc
     1681 atccgcctca acttctacac ggtggaggaa ttggagcagg tggtcacgcg cggtgcgcgg
     1741 gtgctgggcg cgaacatcaa tgccgagggt gctcgagaga tagcgcgtcg ctcgcgcggg
     1801 acgccccgca tcgccgggcg ccttctccgg cgcgtgtggg atttcgctgt cgtcgccaac
     1861 gccaaggtca tcgacgccgc tgtggcggat aaggctctgc gcgcgctcga ggtggacaga
     1921 gccgggctcg atgcgctcga ccaccgctat ctcacctgca ttgcgacgaa ctatgagggt
     1981 ggaccggttg gtatcgagac gatcgcggcg gcgttgtcgg agccgcggga cgcgctcgag
     2041 gagatcgtcg agccgtacct gctgcagcaa ggcttcatcg gccgcacgcc tcgaggccgg
     2101 atcgtgacgc tcaaggccta tcggcacctg ggcctcgccg gccccgcacg atcctcagct
     2161 ccccagatgg gcctcttcga tcccgacaca gacgatggcg cagactccga gactggtgct
     2221 gcaggaaaag atcgcaattc tcgactacgg taatccggcg gataggctag tatcgtccgg
     2281 ctagccaggc ttcaggagtg tgcggtggtc ggagtgggta ggtcggattc ggacggcggt
     2341 ccggccggga gtactgcggc cgcggattac gcgcaaacga acaacactgc tggcatcatt
     2401 gccatgctag cggcgatggc gagtttcgct tgcggcgact cgctcatgaa gctctcctcg
     2461 accgaccttc cgacgggcga gctggtctgg ctccgggcga ccttcatgtc ggcggcaggt
     2521 ttgacctacg ccgtcctcag cggagccgtc cgcagtgcct ggacgctgct tgccaggccg
     2581 atgcttctcc gcgccatcgg tgacaccggc ggggcgctgg cgtatcagtc cgcgctggcg
     2641 cggatggctt tcgctgatct tgtcgccatc aaccaaatca atccgctgac ggtaacggct
     2701 gcctccgcca tctttctcgg cgagcgggta ggctggaggc gatggacggc tgctgcggtt
     2761 ggtctgtgcg gcgtgctgtt gatcatccgc cctggaacaa gcgctttcac atggtggagc
     2821 attgttgcgg tcgtggcagt gctcttcgcg acgctgcgcg acgtctcgac taagagcatc
     2881 gaccgggctg tcccggccgt gctcgtcatg tggttttcct ccagcgctgt ggccttcggc
     2941 agcctcacca tgcttttttt cgagagctgg gtttggcccg agcccgccgt tctgctgcaa
     3001 gtcgccggcg cgagcgcatt ctccctgact gggcatctct gcatcatcac cgccgtgcgg
     3061 aagggagagc tctctgccgt cgctccgttt cgctactcca tcatgatctg ggcgttgctc
     3121 ctcggcctgg tgatctgggg tgagctgccg gacggattag ctatgctcgg aatggccatc
     3181 gtgactggcg ccggcattta caccttccgt cgcgagatgg tcgcgcggcg gaaggcaaga
     3241 ccctagtccg agcgcacaag ctccctcaga tcgacgatcg gctcggtggt gctgtttgtt
     3301 ttactcaatc tatgcgtg
//
LOCUS       DASUMG010000395         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_533516, whole genome shotgun sequence.
ACCESSION   DASUMG010000395 DASUMG010000000
VERSION     DASUMG010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533516"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFX46_06515"
     CDS             complement(<1..899)
                     /locus_tag="VFX46_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HEX5600267.1"
                     /translation="MVNVSFLQELLNTVAEQGRQLLPWVGKSGDTGKDIAELAKALVS
                     IRGEASGVAMARQILTLYRGLSDEQKHAFFRFLANELQPRPEAVQDAASAYLAEPSPA
                     ALSRLQAAVESPRQEFFRRLNLAPGATGEIVALRRDLLRMLARDPSLRVVDADLRHLL
                     YSWFNRGFLVLRRIDWQTPAAILEKIIAYEAVHEIKGWDDLRRRLDPEDRRCFAFFHP
                     SLVDEPLIFVEVALTDDIADNISKLLEEEPKSGSTSEPTTAIFYSISNCQEGLKGISF
                     GNFLIKQVVEELAREKPTLKTFVT"
     gene            complement(1181..1708)
                     /locus_tag="VFX46_06520"
     CDS             complement(1181..1708)
                     /locus_tag="VFX46_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600268.1"
                     /translation="MLRKKLTAERRAEQSAAAVSHPKFREPERLVGLGFRAWLAGYET
                     GDIACWEQAWTMYSDALGPERARAALTDLACWVRAIRTASCRPIEVFPGQCPGFCRDE
                     CMAIAMVAASQHGACPALRACAWALIESSAIDDVVEGAECLGATLKRCRKVLSPASIC
                     GVAALCPLRPHGRPN"
     gene            complement(1796..2848)
                     /locus_tag="VFX46_06525"
     CDS             complement(1796..2848)
                     /locus_tag="VFX46_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX5600269.1"
                     /translation="MTQSIWGRRALRALAPVSLSLAALAFSPVAQANGEVNIYSYREP
                     QLIDPLIKAFTEKTGIKANVVYAGSGLNERLAAEGRNSPADLLFTVDIGRLSEAKTMG
                     LTQPVKSSVLEETIPASYRDPEGHWFGLTLRSRVIYASKERVQQDSITYEELADPKWK
                     GRICSRSGQHAYNVSLIASMIAHKGEEEAEKWLRGVKENLAHKPAGGDREQARDVYSG
                     KCDLAIGNTYYVALMLKNPEQKAWAESIKVLFPNADDRGSHVNISGMALAKHAPNKDN
                     AIKLMEYLASPEAQGIYAQVNNEYPISPEVSPSEVVQSWGKLNPDKLPLDEIARLRKK
                     ASELVDKVDFDAGPSS"
     gene            3178..>3357
                     /locus_tag="VFX46_06530"
     CDS             3178..>3357
                     /locus_tag="VFX46_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX5600270.1"
                     /translation="MTALLGMVAPLSSTLAQEKLLERGRDLAEQHCVRCHAIGLDDES
                     SLPQAPPLRELRNRYP"
BASE COUNT          679 a         1042 c          998 g          638 t
ORIGIN      
        1 gtcacgaacg tcttcagcgt cggcttctcg cgcgccagct cctcgacgac ctgcttgatc
       61 aaaaagttcc cgaacgaaat gcccttcagc ccctcctgac agttggagat cgaatagaag
      121 atcgccgtcg tgggctcgct cgtgctcccc gatttcggct cctcctcgag cagcttcgag
      181 atgttgtccg ctatgtcgtc cgtgagggcg acctcgacga aaatcagtgg ctcatccacc
      241 agcgacggat ggaagaaggc gaagcaccgg cgatcctccg ggtccagccg ccggcgcaga
      301 tcgtcccagc ccttgatctc gtgcaccgcc tcgtaggcga tgatcttctc aaggatcgcc
      361 gctggcgttt gccaatcgat gcggcgcaag accaggaaac ccctgttgaa ccaggagtag
      421 agcaggtgcc tcagatccgc gtcgacaaca cggagtgacg ggtcgcgcgc cagcatcctc
      481 aggagatcgc ggcgcagggc cacgatttcg ccggttgctc ccggagcgag gttgaggcgc
      541 cggaaaaact cttgccgggg actctccacg gctgcctgca ggcgtgacaa ggctgcgggt
      601 gagggctcgg caaggtaggc ggaagcggca tcctgcacgg cctccggccg cggctgcagc
      661 tcattggcga gaaaccggaa gaacgcatgc ttctgctcgt ccgaaaggcc gcgatagagt
      721 gtcaggatct gccgggccat ggccacgccc gatgcctctc cgcggatcga aacaagggcc
      781 ttcgccagct cggcaatgtc cttgcccgta tcaccgctct tgccgaccca aggcagaagc
      841 tgccgtccct gctcggcgac agtgttgagc agctcctgca gaaacgagac gttcaccacg
      901 gtccaacctc ttgcatggtc acccttgttt cgtggccatc tcactcgaaa gcggatgagc
      961 aaccatggct atctagctga caaatgacaa tgcttcgttg gcaggggaca aagaccagct
     1021 ttcagttgct tcccgcaaag cgctacgagg ctagctaccc tgacgcgtgg aatggagcga
     1081 aacccattgc gcgtcaaccg acgcgaccga agatcgcgtc ggtcaacatg gtgaggcaag
     1141 aaccagggat atggatcagg ccgaaccgct cgctttagga tcagttcggg cgaccgtgcg
     1201 ggcgcaatgg gcaaagcgca gcgacgccgc agatcgaggc cggcgacaga acttttcggc
     1261 acctcttgag tgtggcgccc aggcactcgg ccccttccac cacgtcatcg atggcagagc
     1321 tttcaatcag ggcccaggcg caggcgcgca gcgccgggca ggctccgtgc tggctggccg
     1381 ccaccatcgc aatggccatg cactcgtcgc ggcagaagcc cggacattga ccgggaaaga
     1441 cctcgatcgg tctgcagctg gcagtgcgga tggcacggac ccagcacgca agatcggtca
     1501 gcgccgcccg cgcccgctct ggtccgagcg catccgagta catggtccac gcctgttccc
     1561 agcaagcgat gtcgccggtt tcataaccgg caagccaggc tcggaaaccc agcccgacga
     1621 gccgctctgg ctcgcggaat ttgggatgcg aaaccgccgc cgcagattgc tcggcgcgcc
     1681 gctccgctgt cagctttttg cgaagcatac ggtcagatcc gtccgatgga gtccatgagc
     1741 ataaaggcga agcggtttcc gccttgggaa accgcttgca cactgattga acggctcagg
     1801 acgacggccc cgcgtcgaaa tctaccttgt cgacgagctc cgatgccttc ttcctcagcc
     1861 tggcgatctc gtcgagcggt agcttgtccg ggttcaactt gccccaggac tgaacaacct
     1921 cagatggcga aacctctgga ctgatcggat actcgttgtt cacctgcgca tagattccct
     1981 gcgcttccgg cgacgccagg tattccatca gcttgatcgc gttgtccttg ttcggtgcgt
     2041 gcttggcgag cgccatgccg gaaatattga catggctgcc gcgatcgtcg gcgttgggga
     2101 acagcacctt gatcgactca gcccaggcct tttgctccgg gttcttgagc atcagcgcga
     2161 catagtaggt gttgccgata gcgaggtcgc atttgcccga gtagacatcg cgtgcctgct
     2221 cgcgatcccc accggcaggc ttatgcgcca ggttctcctt tacgcccctc agccactttt
     2281 ccgcttcttc ctcgcccttg tgcgcgatca tcgacgcgat gagcgagacg ttgtacgcat
     2341 gctgcccgga tcgagagcag atgcggccct tccacttggg atcggccagc tcctcgtagg
     2401 tgatgctgtc ctgctggacg cgctccttcg acgcatagat cacgcgcgag cgcagggtga
     2461 ggccgaacca gtgaccttcc ggatcacggt acgatgcggg aatggtctcc tcgagcacac
     2521 tcgacttcac cggctgagta agccccatcg tcttggcctc gctgaggcgc ccgatatcga
     2581 ccgtgaacag gaggtcggcg gggctattac ggccctcagc tgcaaggcgc tcattgagcc
     2641 cgcttccggc atagaccacg ttggccttga tacctgtctt ctcagtgaag gccttgatca
     2701 atggatcaat gagctgcggc tcgcggtagg aatagatgtt gacctctccg ttcgcctgag
     2761 cgacaggaga aaatgcaaga gctgcaaggg agagcgaaac gggcgcaagc gcgcgcaggg
     2821 cgcggcggcc ccaaatggac tgggtcatgg cgttctccaa tgtgcggggt tcaaggcccc
     2881 gtatcggaaa ccaagcgccg ggttgttccg gcgcgcaacc aaatcgcctc aacacgcagt
     2941 cactgtcaag gtgcagcaag cattgaagga cggaaatact tcaaacccgg ttggcattac
     3001 caattcattt tgaattagaa ctattctcaa ttgagattaa ttgagagccg atattgatgc
     3061 attgtttcgc actgatcgaa tgaaatgggc ataatcggcc gcacaaccgg gttgattgat
     3121 atacgggcaa gcatcatgac cagcagcgcg aaaggagccc cacggatcat tacactgatg
     3181 accgcactgc tgggcatggt ggcaccgttg agttctacac tcgcccagga gaagctcctg
     3241 gaacgcggac gcgacctcgc cgaacagcac tgtgtccggt gtcacgcaat cggcctggac
     3301 gacgaaagca gcctgccgca ggcgccgcca ttgcgcgagc tgcgaaaccg ctatccg
//
LOCUS       DASUMG010000396         2919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_774015, whole genome shotgun sequence.
ACCESSION   DASUMG010000396 DASUMG010000000
VERSION     DASUMG010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774015"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFX46_06535"
     CDS             complement(<1..881)
                     /locus_tag="VFX46_06535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX5600271.1"
                     /translation="MHDHNHHGSFVHYSRRVLDREFWRKRRRRLVARAITGATGLLAS
                     TPRLRGLALRALLDRHLAPGTLCLVPFGDHALYVDPRDDKIALKLMRGKCWQRRELET
                     ALEVLRSAGTLRAGGMFIDVGANIGALAVYALKSGVFSKVIAAEPDPHNFAILKRNVA
                     LNGFEQRVHAIEAAVSNTNGHLMLARHRKNYGAHSVEGSARINATQTITVDAVTLDEV
                     LRQQDVAPEEVALVKIDVEGHELSVLEGMGSLLQARVPILVELTVEYGDDERLGKFKA
                     LLAPFYERVLDLSDETWA"
     gene            1196..2410
                     /locus_tag="VFX46_06540"
     CDS             1196..2410
                     /locus_tag="VFX46_06540"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX5600272.1"
                     /translation="MKKIKVTGTVVELDGDEMTRIIWKLIKEKLIYPYLDVNLEYYDL
                     GIENRDRTNDQVTIDAAHAIKKHGVGVKCATITPDEARVKEFGLKQMWKSPNGTIRNI
                     LGGVIFREPIICKNIPRLVPGWTQPIIIGRHAFGDQYRATDFKFPGKGKLTIKFEGED
                     GTVIEREVFNAPGSGVALAMYNLDASIRDFARASFNYALARNYPLYLSTKNTILKEYD
                     GRFKDIFQEIYENEFKAEFEKRGLTYEHRLIDDMVASALKWSGGYVWACKNYDGDVQS
                     DSVAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREYQKGRETSTNSIASIFAW
                     TRGLAHRAKLDDNPELANFAQTLERVCIETVEAGYMTKDLALLVGADQKWLSTTGFLD
                     KVEENLRKAMGA"
     gene            2591..>2919
                     /locus_tag="VFX46_06545"
     CDS             2591..>2919
                     /locus_tag="VFX46_06545"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012652796.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX5600273.1"
                     /translation="MTTTASDPVVIVSAARTPIGSFQGAFASLSAPELGAIAVKAAVE
                     RAGVEPDEISEVLMGCVLPAGQGQAPARQASRGAGLPDHVPCTTVNKMCGSGMKTVMM
                     AYESLHAR"
BASE COUNT          609 a          934 c          856 g          520 t
ORIGIN      
        1 gcccacgttt cgtccgagag atccagcacg cgttcgtaga agggagccaa caaggccttg
       61 aacttcccga gccgctcgtc gtcgccatac tcaacagtga gctcgacgag aataggaacc
      121 cgagcttgca ggagcgaccc catcccctcg agcacggaca gctcgtggcc ctccacgtcg
      181 attttcacca gcgccacctc ctcgggcgcg acgtcctgct gcctcagcac ttcgtcgagc
      241 gtaacggcgt ctactgtgat agtctgtgtg gcgttgatgc gtgcgctgcc ctcaacggag
      301 tgagcgccgt agttcttgcg gtgacgcgcg agcatgagat gaccattcgt gttgctcacc
      361 gcagcctcta tagcatgcac acgctgctcg aagccgttca gagcgacatt gcgcttcaat
      421 attgcaaagt tgtggggatc cggctctgcg gcaatgacct tcgagaaaac gcctgactta
      481 agggcataga cagccagtgc acctatgttg gcgccaacat cgatgaacat accgccggcg
      541 cgtaaggtcc ccgcagatcg cagcacctca agcgcggttt ccagctcgcg gcgctgccaa
      601 cattttccgc gcataagctt cagtgcgatc ttgtcatcgc ggggatcaac gtagagtgca
      661 tggtcgccaa acgggacaag acagagcgtt cctggtgcca ggtggcgatc aagcaaagca
      721 cggagcgcaa ggccacgtag tcgcggcgtt gatgcgagca gccccgtcgc gcccgtgatg
      781 gcacgcgcga ccagccggcg acgtcttttg cgccagaact cgcgatccag cactcgacga
      841 ctgtaatgca cgaagcttcc gtggtgatta tgatcgtgca atcaactttc gtaggccgct
      901 ttggttcgat tgcgccggca cattaaacgg gtattggtaa cgcgcaccgt cagtaaacag
      961 agcaaatcct cgtgcattgt tggctgccaa atctcgatca aaccaatgga agcgacttcg
     1021 cggattggaa gctgtccagg agcacctttc gaaccgttgc ctggcacctt gggacgtccc
     1081 gccccaagcc acttggattc ccgcgccgtt ccgtctatga ccccgggcga caggcaccgg
     1141 ccgacagccg gcgccgccaa gtctttagaa gcccgcgcga tctggtgaga acgacatgaa
     1201 gaagatcaag gtcacaggaa ccgtcgtcga gctcgacggc gacgagatga ctcgcatcat
     1261 ctggaagctc atcaaggaga agctcatcta cccctacctt gatgtgaatc tcgagtacta
     1321 cgacctcggc atcgagaacc gggaccgcac caacgaccaa gtcacgatcg acgcggcgca
     1381 cgccatcaag aagcacggcg tcggcgtaaa atgcgccacc atcacgccgg atgaagcgcg
     1441 ggtgaaggaa ttcggcctca agcagatgtg gaagtcgccg aacggcacca tccgtaacat
     1501 cctcggcggc gtcatcttcc gcgagcccat catctgcaag aacatcccgc ggctggtgcc
     1561 gggctggacg cagccgatca tcatcgggcg tcatgccttc ggcgaccaat accgcgccac
     1621 cgacttcaag ttcccaggca agggcaagct gacgatcaag ttcgaaggag aggatggcac
     1681 cgtcatcgag cgcgaggtct ttaacgcgcc gggctcgggc gttgccttgg caatgtacaa
     1741 cctcgacgcc tccatccggg atttcgcgcg cgcctcgttc aactacgccc tcgcgcggaa
     1801 ttatcccctc tacctctcga ccaagaacac catcctcaag gagtacgacg gccgcttcaa
     1861 ggacatcttc caggagatct acgaaaacga gttcaaggcg gagttcgaga agcgcggcct
     1921 cacctacgag caccgtctga tcgacgacat ggtcgcctcg gcgctgaagt ggtcgggcgg
     1981 ctacgtctgg gcctgtaaga actatgatgg cgacgtgcag tccgactcgg tggcccaggg
     2041 cttcggctcg ctcggtctca tgacgagcgt cctgatgacg cccgatggca agaccgtaga
     2101 ggccgaggcg gctcacggca cggtgacgcg tcactaccgc gagtatcaga agggcagaga
     2161 gacctccacc aactcgatcg cttcgatctt cgcctggacc cgcggactcg cccaccgcgc
     2221 caagctcgat gacaaccccg agctcgcgaa cttcgcgcag acgctggagc gggtctgcat
     2281 cgagaccgtc gaggccggct acatgacgaa ggacctcgca ctcctcgtcg gcgccgacca
     2341 gaagtggctc tcgaccaccg gcttcctcga caaggtcgaa gagaacctgc ggaaggctat
     2401 gggggcctga ggcgttcgcg cctcagaatg aacaccttgg gcgcttccga ggcgcccaag
     2461 ggccaatgcc cgaaccgccg tttcgatcag acagcggatc acgcccatgc cgcgttgacc
     2521 tcgctgccgc ccggtgttgt gatgcgttgc aaaactccat cgagcgaggc catcgagcga
     2581 ggctccaccc atgacaacga ccgcttctga tcctgtcgtc atcgtctcag ccgcgcggac
     2641 acctatcggc agctttcagg gtgcgttcgc aagcctttct gctccggagc ttggcgctat
     2701 tgccgtcaag gctgccgtcg agcgggcggg cgtcgagccc gatgaaatca gcgaggtcct
     2761 gatgggctgt gtgctaccgg cgggccaggg ccaagctccc gcacggcagg cctcccgcgg
     2821 cgccggattg cccgaccatg tgccctgcac gaccgtcaac aagatgtgcg ggtccggcat
     2881 gaagacagtc atgatggctt acgagagcct gcatgcccg
//
LOCUS       DASUMG010000397         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1176165, whole genome shotgun sequence.
ACCESSION   DASUMG010000397 DASUMG010000000
VERSION     DASUMG010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176165"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VFX46_06550"
     CDS             complement(<1..1195)
                     /locus_tag="VFX46_06550"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEX5600274.1"
                     /translation="MAQRDKVSNSSQQWAPETLLVHGGQLRTEFGETAEALFLTQSYV
                     YDSAEQAEARFAGDEPGFIYTRFSNPTVAMFEERMRLLEGAEAARATATGMAAVTAAL
                     LSYLRTGDHIVAARAMFGSCRYIVDELCPRFGIETTLVDGSDVEQWRKALRPNTKAVF
                     FETPANPTLEVVDIAAVSALAHDVGALVFVDNVFASPILQRPLALGADVVMYSATKHI
                     DGQGRCLGGVVLCSEKFLKDYLHTFLRQTGPSLSPFNAWVLLKGLETLPLRVNAQCAT
                     AAKIADWLAQQSAVERVLYCGRSDHPQAVIIKRQMSGGGQVVTFEIVGGKERAFRFVN
                     ALRLIRISNNLGDAKSLITHPATTTHQRLSPEARAELGITDSMLRLSVGLEAAEDLIA
                     DLDAGL"
     regulatory      complement(1272..1349)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1503..2606
                     /locus_tag="VFX46_06555"
     CDS             1503..2606
                     /locus_tag="VFX46_06555"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HEX5600275.1"
                     /translation="MADQAASEKANGILPAQAIRRLIADGHIQLAEAMQDKQLQPASL
                     DLRLGECAWRVRASFLPGPEARVEDKLAELALHKIDLRQSAVLETGCVYVAPLLERLN
                     LPPDVSAAANPKSSTGRLDVFTRVIADGVASFDQVPAGYSGPLYAEICPQTFPIIVRR
                     GSRLSQVRFRQGTFADSDAALADLHRRERLVSSDKPDISHGIALSVDLAGDENGLVGY
                     RAKRHTGLVDVDATGSCAILDYWEPITLHGEKRLILDPDEFYILASREAVNVPPTHAA
                     EMVPFNPLVGEFRVHYAGFFDPGFGHAASGGAGSRAVLEVRSHKVPFILEHGQIIGRL
                     IYERMTEVPEMLYGAALGSHYQAQGLKLSKHFK"
     gene            complement(2619..2960)
                     /locus_tag="VFX46_06560"
     CDS             complement(2619..2960)
                     /locus_tag="VFX46_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX5600276.1"
                     /translation="MQRPFVHPPIEDVALESVLYALADPVRLAIMLKLSSGGCPMNCS
                     TAAPERLPKSTQSHHYKVLREAGLIRSERRGTEVVNTLRCDEINAKFPGVLPAILKAA
                     AAQFDRATEDA"
     gene            complement(3014..>3915)
                     /gene="dapE"
                     /locus_tag="VFX46_06565"
     CDS             complement(3014..>3915)
                     /gene="dapE"
                     /locus_tag="VFX46_06565"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX5600277.1"
                     /translation="RDGVLYGRGAVDMKGAIACFVAAALRYLDAHNGKPAGSISLLIT
                     GDEEGPSINGTAKVLKWLEARGERLDACVVGEPTNPHTIGEEIKIGRRGSLNGELVVY
                     GKQGHAAYPHLADNPVPKLARIIDRLSTTPLDEGTPDFEPSSLQVTVISVPNTATNVI
                     PSEARAKFNIRYNDLHRRPDIEAWVRQQCSAAANEVGARWSVSFSGTGDVFLTRPGPL
                     VDTMVEAVRQVTGREPRLTTGGGTSDARFIQAYCPVIEFGLVNETIHQVDERVPLDDL
                     HQLTAIYERFLAEYFSKAVANAS"
BASE COUNT          742 a         1245 c         1227 g          701 t
ORIGIN      
        1 cgagacctgc atcgaggtca gcgatgagat cctccgccgc ctcgagcccg acggacaacc
       61 gcaacatgct atccgtaatg ccgagctcag cgcgtgcctc cggactaagg cgctggtggg
      121 tcgtcgttgc cgggtgagtg atgagactct ttgcgtctcc cagattgttg gagattcgga
      181 tgagcctcag cgcattgacg aatcggaagg cccgctcctt tccgccgacg atctcgaagg
      241 tcaccacttg tccgccgccc gacatctggc gcttgatgat taccgcttgg gggtgatcgg
      301 agcggccaca atagagcacc cgctcaacgg cggactgctg cgccagccaa tcagcaatct
      361 tcgcggctgt ggcgcattga gcgttcaccc gcagtggcag cgtctccaat cccttcagca
      421 acacccaggc gttgaacggg ctgagcgaag gcccggtctg gcgcaggaac gtgtgaaggt
      481 aatcctttag gaatttctcc gagcacagca cgacgccgcc gaggcaccgt ccctggccgt
      541 cgatatgctt cgtagccgaa tacatgacca cgtcggcgcc aagcgcgagc ggcctctgga
      601 gaattggcga ggcaaaaaca ttatcgacaa aaacaagcgc tccaacgtcg tgcgccaatg
      661 ccgaaactgc tgcgatgtcg accacctcga gagtcggatt ggccggcgtt tcgaagaaaa
      721 cagctttcgt gttgggccgt agggccttcc gccattgctc gacatcgctg ccgtcgacga
      781 gagtcgtctc gatgccgaag cggggacaga gctcatcaac gatgtaacgg caggaaccaa
      841 acatcgcgcg cgccgcgacg atgtgatcac cggtccggag ataactcagc aaggccgccg
      901 tgactgccgc catcccggtc gccgtcgcgc gcgccgcttc ggcgccttcc agcagccgca
      961 tacgctcttc gaacatcgcg acggtggggt tggaaaatcg cgtatagatg aacccgggct
     1021 cgtcgccagc gaagcgggcc tcggcctgct cagcggagtc gtagacgtag ctctgggtga
     1081 gaaagagcgc ctcggctgtc tcgccaaact ccgtccggag ctgtccgccg tggacgagga
     1141 gcgtttcggg cgcccattgc tgactggaat tcgaaacctt atcgcgctgc gccatggacg
     1201 cctccttacc tgctggcccc agggcaaaaa gaaaacccgg ccgcagatct cgcaaaatca
     1261 ccgagcgcgg gccaggtttg cgcgcggcct tttagcgagt tctttaacgt ggctgcaagc
     1321 cggccggcca aatcaccacg atgcgctgcg ctgatacgcc tgatgcgaac gatcgtcaag
     1381 gtcggagtcc aaacaattat cagcaagctg ctccgcgagc ttacggagca cagcgcgaga
     1441 cacaggcgcg ccttggcgtg gaagggttga gcggctacac acgtcgcctg cggaggtgcg
     1501 gcatggctga tcaggcagct tcagaaaaag cgaatggaat tttgcctgca caggcgatac
     1561 ggcggctgat cgctgacggg catatccagc tcgcggaggc gatgcaggac aagcagcttc
     1621 aaccggcgag tctcgatctg agactcggtg aatgtgcttg gcgcgtgcgg gcgagcttcc
     1681 tgcccggtcc cgaagcgagg gtcgaggaca agctcgccga gctggccctg cacaagatcg
     1741 acttgaggca gtcggcggtg ctcgagacag gatgcgtcta cgtcgcaccg ctgttggaac
     1801 ggcttaacct gcctccggat gtctccgcag cggccaaccc gaagagctct acgggacggc
     1861 ttgatgtctt cacgcgcgtt atcgccgatg gcgtcgcgtc gttcgaccaa gtgcccgccg
     1921 gctactccgg acctctctac gccgagatct gccctcaaac attccccatc atcgtgcggc
     1981 gaggctcgcg cctgagccag gtgcggtttc gccaggggac attcgcggac agcgacgcgg
     2041 cgctagctga cctgcatcgg cgcgaaaggc tcgtgtcctc cgacaagccg gacatctcgc
     2101 acggaatcgc cctatcggtc gatctggcag gcgatgagaa cggcttggtt ggctatcgcg
     2161 cgaagcgtca caccgggctc gtcgacgtcg atgcgaccgg ctcgtgcgcg atcctggatt
     2221 actgggaacc catcaccctt cacggcgaga agcggctcat cctcgatccc gacgagttct
     2281 acattctcgc ctcgcgtgag gccgtgaacg tgccgccgac gcacgcggcg gagatggtgc
     2341 ccttcaatcc gctcgttgga gagttccggg tgcactacgc cggcttcttc gaccccggat
     2401 ttggacacgc cgcatcaggc ggcgccggct cacgggcggt actcgaggtg cgctcgcaca
     2461 aggtgccgtt catcctggag cacggccaga tcatcggccg gctgatctac gagcgcatga
     2521 cagaggtgcc ggagatgctc tacggcgcag cccttggcag ccattaccag gcgcaggggc
     2581 tgaagctgtc gaaacacttc aagtgaggag cagaacgctc acgcgtcttc cgtcgcccgg
     2641 tcaaactgcg ccgccgcggc cttcagaatg gcgggcaaga cacccgggaa ctttgcgttg
     2701 atctcgtcgc aacgcaacgt gttgacaacc tccgttcccc ggcgctccga tcggatgaga
     2761 cctgcttcgc gcagcacctt gtagtgatgc gactgggtcg acttcggcaa cctctccggc
     2821 gctgctgtcg aacagttcat cgggcacccg cccgacgaga gcttcagcat gattgcaaga
     2881 cgcacgggat cggcaagcgc gtagagaacg ctctccagcg ccacatcctc gatcggcgga
     2941 tgcacaaagg gccgttgcat gcccgtagta tgagccaccc gagcaacttg ttcaataaac
     3001 cggagaagtg gaactagctc gcattcgcaa cagctttgct gaaatactct gccaggaacc
     3061 gctcataaat ggccgtgagc tgatgcaaat cgtccagcgg cacccgctcg tccacctggt
     3121 ggatcgtctc gttgaccagg ccgaactcga tcacagggca ataggcctgg atgaaccggg
     3181 catcggaggt gccgccgcca gtcgtcaacc ggggctcgcg ccccgtgacc tgccgcacgg
     3241 cctcgaccat cgtatcgacc agcggccccg ggcgggtcag gaacacgtct cctgtccccg
     3301 aaaagctgac gctccatcgg gcgccgacct cgttcgcggc ggcggagcac tgctgccgga
     3361 cccaagcctc gatgtccggg cggcggtgca ggtcgttgta gcggatgttg aacttcgcgc
     3421 gcgcctcgga gggaatgacg ttcgtcgccg tgttgggaac ggagataacc gtcacctgga
     3481 ggctggacgg ctcgaagtct ggtgtcccct cgtccagcgg tgtcgtcgat agacggtcga
     3541 tgatgcgggc gagcttgggc accgggttgt ccgcgaggtg cgggtacgcg gcgtggcctt
     3601 gctttccata gacgacaagc tcgccattga ggctgccgcg gcggccgatc ttgatctcct
     3661 caccaatcgt gtgaggattc gtcggctctc cgaccacgca ggcgtcgagc cgctcgcccc
     3721 tggcctcgag ccatttgagc accttcgccg tgccgttgat cgaaggcccc tcctcgtcgc
     3781 cggtgatgag caggctgatc gagcctgccg gcttcccgtt gtgagcgtcg aggtagcgca
     3841 gagcagccgc aacgaagcag gcgatcgcgc ccttcatgtc gacggcaccg cggccgtaga
     3901 ggacaccgtc gcgga
//
LOCUS       DASUMG010000398         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_212951, whole genome shotgun sequence.
ACCESSION   DASUMG010000398 DASUMG010000000
VERSION     DASUMG010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212951"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VFX46_06570"
     CDS             <1..490
                     /locus_tag="VFX46_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5600278.1"
                     /translation="SISAEETFKPARDRKKMSHYAHSAVFAEVRVDEELGVVRVPRIV
                     CAAAAGRIINPKTAGSQIIGGIVMGLGMALEEEALTDHRIGRIMNHNLAEYHVPVHAD
                     VGNIDVIFVDEPDPHVSPLGVKGLGELGIVGAAAAIANGIFHATGKRIRDLPITIDKL
                     LA"
     gene            804..1898
                     /locus_tag="VFX46_06575"
     CDS             804..1898
                     /locus_tag="VFX46_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX5600279.1"
                     /translation="MRRLLPLLLALVVAGGLAIALLPDGRLALRAEKSGKQTPRQSQA
                     VAVEVAPAIAARYQEDLRSVGSLMSDESVQIASEIAGRIQSFEFAEGQAVKAGDVLVK
                     LDDSLARAELADAKARFELARANRERAQLLRKQGHMSQQAHDEAAANFLIAEAALELA
                     SVRLSKHVIKAPFDGIVGLRNASAGAYVNIGTPIVNLEKIDFLKIDFKLPELALTQIS
                     TGQDVIVEVDAVPGRTFKAQIYAISPMVDVNGRALHVRARMANPDLLLRPGLFARITV
                     KGRETREVVAVPESAIVPRGGESFVYVIQGGRAVESKVKLGKRENGTVQVLSGLSAGS
                     IVVVAGQLKLKDGAPVEVVQTVSGPEPEKS"
     gene            1898..>2278
                     /locus_tag="VFX46_06580"
     CDS             1898..>2278
                     /locus_tag="VFX46_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX5600280.1"
                     /translation="MSPIEVCIRRPVFATVLSLVLVLLGLVSYTRLTVREYPNIDEPT
                     VSVTTMYPGAAPHIVETQVTQVLEGSLAGIPGIDVIESTSRSETSRITIRFRQDVNPD
                     EAANDVRDRVSRVRGRLPDEIQEPV"
BASE COUNT          436 a          709 c          723 g          410 t
ORIGIN      
        1 ctccatttcg gcggaggaaa cgttcaagcc ggcccgggac aggaagaaga tgtcccatta
       61 cgcccacagc gccgtcttcg ccgaggtccg cgtcgacgag gagctgggtg tggtccgggt
      121 gccgcggatc gtctgcgccg ctgctgccgg ccgcattatc aaccccaaga ccgcgggcag
      181 ccagatcatc ggcgggattg tgatgggcct tgggatggcg ctggaggagg aggcgctgac
      241 cgatcaccgc atcggccgca tcatgaacca taatctcgcc gagtatcatg tcccggtgca
      301 tgccgatgtc ggtaacatag atgtgatctt cgttgacgag cccgaccccc acgtttcgcc
      361 gcttggcgta aaggggctgg gcgagctcgg gatcgtcggc gcggccgcgg caatcgccaa
      421 cggcatattc cacgccaccg gcaagaggat ccgcgacttg ccgatcacga tcgataagct
      481 gctggcctga gaaataggtc tgacaccggt atcaccccgc tatcccggta cctcgttcga
      541 cgggtgctcg gctgcgcgcg ctcacccgcg cctccaatcg ggccaacgaa attccgcatc
      601 accccattgg gctaggctct cgacgccgaa ttgcgggata aactttcgcc aagaagcagt
      661 tgtgctctgt tgcggacttc gtccgatgta tactgtcgcg acgggttccg aagcgctgat
      721 ccgttctgag agagctagcg cttcgaaaaa ccgagccagc aacaagaaca cgaagaccgc
      781 tcccaggggg tgaggacgac gcgatgcgaa ggcttttgcc gctccttttg gcgctcgtgg
      841 ttgccggtgg actggcgatc gcgttgttgc cagatggccg cttggcgctg cgcgccgaaa
      901 agagcggcaa gcagacgccc cgccagagcc aggctgtcgc cgtggaggtg gcgcccgcaa
      961 tcgcagccag gtaccaggag gacctgcgct ccgtcggcag cctcatgtcg gatgagtcgg
     1021 tccagattgc ctcggaaatc gctggccgca tccaaagctt cgagtttgcg gaaggacagg
     1081 ccgtcaaagc cggagacgtg ctcgtcaagc tcgatgactc tttggcgcgt gccgaactgg
     1141 cggatgcaaa agcgcgattc gagcttgcac gtgccaaccg agagcgtgcg cagttgctga
     1201 gaaagcaggg tcacatgtcg cagcaggcgc acgacgaggc tgcggcgaac tttctgatcg
     1261 cggaggccgc acttgagctg gcctcggtca gactctccaa gcacgtcatc aaggcgccct
     1321 tcgacggcat cgtgggactg cgaaacgctt ccgccggtgc ctacgtgaac atcggcacac
     1381 caatcgtaaa cttggagaag atcgacttcc tgaagattga cttcaaactg ccggagcttg
     1441 ccctaacgca gatcagcact gggcaggacg tcatcgtgga agtcgatgcc gtgcccggcc
     1501 ggacgttcaa ggcacagatc tacgccatca gccccatggt cgacgtcaac ggccgtgcgc
     1561 tgcatgtgcg ggcccgcatg gccaatccgg atcttctgct gcggccgggc cttttcgctc
     1621 ggatcaccgt gaaagggcga gaaaccaggg aagtggtggc ggtgcctgaa agcgcgatcg
     1681 tcccccgcgg tggtgaatca tttgtctacg tcattcaggg cggaagggcg gtcgaatcta
     1741 aggttaagct gggcaagcgc gagaacggca ccgtccaggt gctttcgggc ctcagcgccg
     1801 ggtccattgt cgtcgtcgcc ggtcagctca agctcaagga tggcgccccc gtcgaggtcg
     1861 tccagaccgt gagcggccca gagccggaga agagctgatg agccccatcg aggtctgcat
     1921 ccggcgccct gtttttgcga cggtcctcag cctcgtcttg gtgctgctcg gtctcgtctc
     1981 gtacacgcgg ctgacggttc gcgagtaccc gaacatcgac gagccgaccg tttcggtcac
     2041 gacgatgtat cccggcgccg cgccgcatat cgtcgagacg caagttactc aagtcctcga
     2101 aggctcgctg gcgggcattc ccggcatcga cgtcatcgag tctaccagcc gctcggagac
     2161 gagccgcatc accattcgct tccggcaaga cgtcaatccc gacgaggccg ccaacgacgt
     2221 gcgtgaccgg gtcagccgcg ttcgcggacg actacccgac gagatccagg agccggtc
//
LOCUS       DASUMG010000399         3379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_452884, whole genome shotgun sequence.
ACCESSION   DASUMG010000399 DASUMG010000000
VERSION     DASUMG010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452884"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VFX46_06585"
     CDS             <1..217
                     /locus_tag="VFX46_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX5600281.1"
                     /translation="MVVACHIEVGYCHTGGMPMSVHMFCLSSRRATARIYAGGRAEFR
                     TDPMWFTDYSRRATVEWERPEQVEAAE"
     gene            345..1442
                     /locus_tag="VFX46_06590"
     CDS             345..1442
                     /locus_tag="VFX46_06590"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX5600282.1"
                     /translation="MRAAVLTEVNKPLEILDLEQEGPKAGEARVKVKATGVCMSDWHI
                     MNGDWPLPLPIVPGHEAAGEVVELGPGVDHLRVGQHVIFSFRPHCGHCPYCSQGRTVL
                     CSGHNDTPRWRMHDGTSRLKLNGEPVNVMARIGTFSEYVVCPAEQLVPIREDLPWTHA
                     AIIGCSVATGVGAVVRHAQVPAGSSVLVIGCGGVGLNVVQGAKLAGARMIIAADLLDN
                     KLQYAKHFGATHTINAREEDVVKCVRDLTGGFGVEYAFDAIGGSQTAAQAVDAVAPGG
                     HAVLVGIPASTATAAIGLMNMVYMEKKITGTYYGSVRPNIDFGILADLTMDKRLNLDD
                     LISRTYRFEEINEGFRLLTSGSVARGVIVFD"
     gene            1505..2185
                     /locus_tag="VFX46_06595"
     CDS             1505..2185
                     /locus_tag="VFX46_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5600283.1"
                     /translation="MTATTSSLKTVHLNLPASTDELRKLEIGTVAYLTGRIFTAREGV
                     YKRAVEDGYGLPASPAELGSANFHCSPAATIEPDGSYTVGAVTATASFRFAKWLDGWF
                     KLSGINIVIGKGGMTSETYKRSFVPNNAIYLTTVGYGTGALLGRGIKRVAGVHWLEEL
                     GLAQAIWVLEVENFGPFLVESDLDGNSLFERENAKIAAGLDKVYAGTRPAILRRYGET
                     DDKTEEVI"
     gene            2264..2719
                     /locus_tag="VFX46_06600"
     CDS             2264..2719
                     /locus_tag="VFX46_06600"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX5600284.1"
                     /translation="MAEETISAPPRTRARYNHWTTVSIRYCDQDPLGHINNVAMAAYL
                     EQARVGLIYPLLKSKGGPNFELVIARLVIDYLNELHFPGNVEIGTRIARLGNKSFTLH
                     HAVFMAGEEVCAATSEAISVFFDMSKRESVMPTPALRAALEEFMQRQPG"
     gene            complement(2878..>3379)
                     /locus_tag="VFX46_06605"
     CDS             complement(2878..>3379)
                     /locus_tag="VFX46_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporting ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HEX5600285.1"
                     /translation="RDVMARPPRSASEPIITRFGLWRMILVSLLMAGPAFGLFVYDLS
                     RGADLDEARTIVINVIVMAEAFYLFNARYLRESSFNFAGIFGSQPVLIGFGVAVLLQM
                     AVTYLPPMQFLFGTTSLGIIEWTRIIAICFAIFCIVELEKAVRRLWANRKNAARNAHS
                     FSQSNR"
BASE COUNT          671 a         1005 c         1047 g          656 t
ORIGIN      
        1 gatggttgtc gcctgccaca tcgaggtcgg ctactgccac acgggcggca tgccgatgag
       61 cgtgcacatg ttctgcctct catcacgccg cgcgacggcg cgcatttatg ccggtggtcg
      121 cgctgagttc cgcaccgatc cgatgtggtt caccgattac tctcggcggg caacggtcga
      181 atgggagcgg ccggagcaag tcgaggcggc ggaatagcgc caagtgcccc agcgctgaac
      241 atcttagctt ccgccttctc ggactttagg gcgggtttgc agttggcgct ggtagccgga
      301 gcggcgtaat gtgacaacat tccggaaacg ggaggagcga cccaatgcgc gctgcagttc
      361 taaccgaggt caataagcca ctcgaaattc tcgatctgga gcaggagggg ccaaaggccg
      421 gcgaggcccg cgtcaaggtg aaggccaccg gcgtttgcat gagcgactgg cacatcatga
      481 atggcgactg gccgttgcca ttgccgatcg tgccaggtca tgaggcggcg ggcgaggtcg
      541 tggagcttgg ccctggggtt gatcatttgc gtgttggcca gcacgtcatc ttctcgttcc
      601 gcccgcattg cggacattgc ccatactgct cgcaggggcg cacggtgttg tgctctggcc
      661 ataacgacac gcctcgctgg cggatgcacg acggcacctc acgcctgaag ctcaatggtg
      721 agccggtcaa cgtcatggcc cggatcggca ccttctccga gtacgtcgtc tgcccggctg
      781 agcagctcgt gccgatccga gaggatcttc cgtggacgca tgctgcgatc atcggctgca
      841 gtgtcgctac gggcgtcggt gctgtggttc gtcacgcaca agtgcccgcc ggctcttctg
      901 tgctcgtcat cggctgtggc ggggtcgggc tcaatgtggt gcaaggtgcc aagctggccg
      961 gtgcgcggat gatcatcgcc gccgatctgc tcgacaacaa gctgcagtat gccaagcact
     1021 tcggtgcgac ccacacgatc aacgcgcgcg aagaggacgt ggtgaaatgc gtccgcgatc
     1081 tgaccggagg cttcggagta gagtacgcct tcgatgccat cggtggttcg caaacggccg
     1141 cgcaggccgt cgatgctgtc gctcctggtg gccacgcggt gttggtcggc attcctgcct
     1201 ccactgccac ggcggcgatc gggctgatga acatggtcta catggagaag aagatcactg
     1261 ggacctacta cggctcggtg cggccgaaca tcgatttcgg catcttggcc gatctgacca
     1321 tggacaagcg cctcaacctc gatgatctca tcagccgcac ttaccgcttc gaggagatca
     1381 acgaaggttt ccggctcttg acgtcgggca gcgttgctcg cggtgtgatc gtgttcgact
     1441 gatcggaggc agaacgttcg gcgagctgga ccaatcagac cctcacggag gcgaaactgt
     1501 actgatgaca gccaccactt cgagcctgaa gaccgttcac ctgaacctcc cggcctccac
     1561 ggacgagctg cggaagctgg agatcggtac ggtggcctat ctcaccggcc gcatcttcac
     1621 cgcgcgcgag ggggtctata agagggccgt cgaagatgga tacggcctgc ccgcatctcc
     1681 agccgagctg ggcagcgcca atttccattg ctcgccggcg gccaccatcg agcccgatgg
     1741 cagctacacg gttggtgcgg tgacggcgac ggcgtcgttc aggttcgcca agtggttgga
     1801 tggctggttc aagctctccg gcatcaacat cgttatcggc aaggggggca tgacgagcga
     1861 gacctacaag cgctcgttcg tgcccaacaa tgccatctac ctaacaacgg tcggctacgg
     1921 cacgggggca ttgctcggcc gagggatcaa gcgggttgcc ggtgtgcatt ggctggagga
     1981 gctgggtctc gcccaagcga tctgggtgct cgaggtcgag aatttcgggc cattcctcgt
     2041 tgaaagcgat ctcgacggca acagcctgtt cgaacgcgaa aatgcgaaga tcgcagcggg
     2101 actggacaag gtttatgccg gcacgcgccc ggccatcctg cgccgctatg gcgaaacgga
     2161 tgacaaaacg gaggaggtga tttgaggccg aactcctcca gctctcaatg ataggcgtgc
     2221 cagctatccg cccgatttca agctaaagtc gatcgtctcg aagatggcag aagaaaccat
     2281 ttcggcgccg ccccgcacgc gcgcccgcta taaccactgg acaaccgtct ccatccgcta
     2341 ttgtgaccag gaccccctgg ggcacatcaa caatgttgcc atggccgcgt acctggagca
     2401 ggccagggtt ggcctcattt acccgctgct gaagagtaag ggcgggccga atttcgagtt
     2461 ggtcatcgcc cgcctcgtca tcgattacct caacgagctg cactttcccg gcaacgtcga
     2521 gatcggaacg cgtatcgccc ggctggggaa taaatctttc acgctgcatc acgcggtgtt
     2581 catggccggc gaggaggttt gcgccgcgac gtccgaagcc atctcggtgt tcttcgacat
     2641 gtccaagcgg gaatcggtga tgccgacgcc tgctcttcgc gcagcgctgg aagagttcat
     2701 gcagcggcag ccagggtaga gcgggtgttg cgaaactgcc gctacctttt ttctccttac
     2761 acggattatg gaaccaagca agcgctcgcg catttactag gcgtctatcg gatgatcaca
     2821 cgacaggcag cggggtgtgc gccccggaga gcgatacggc cgacccctct ccggggctta
     2881 cctatttgac tgggaaaacg aatgcgcatt acgagccgcg tttttgcggt tcgcccagag
     2941 tctccgcact gccttttcca gctcgacaat gcagaagatc gcgaagcaga tcgcgatgat
     3001 gcgtgtccac tcaataattc caaggcttgt ggtgccgaac aagaactgca tcggcggcaa
     3061 ataagtgacc gccatctgca gaaggactgc cacaccgaat ccgatgagca cgggctggct
     3121 gccaaaaata cccgcaaagt tgaacgagct ttccctgaga tagcgagcat tgaagagata
     3181 aaaggcttcg gccatgacga tcacgttgat gacgatcgtc cgtgcctcat ccagatccgc
     3241 ccctcgggac aagtcgtata cgaataggcc gaacgccggc cctgccatca gcagtgacac
     3301 caggatcatc cgccataggc caaatcgcgt gatgattggc tcggaggccg acctgggcgg
     3361 gcgcgccatc acatccctc
//
LOCUS       DASUMG010000400         2673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_935783, whole genome shotgun sequence.
ACCESSION   DASUMG010000400 DASUMG010000000
VERSION     DASUMG010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935783"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..910)
                     /locus_tag="VFX46_06610"
     CDS             complement(128..910)
                     /locus_tag="VFX46_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX5600286.1"
                     /translation="MKTVDRVATILRLLSAAGDGGFRLSDIAETTGLGKTTAHRLLGA
                     LVNVGFVHQDLKTKRYRLGASAAALGRDAAIHHIGSLVRPVLRRLAAETEDTVFASVR
                     EGLAAVCIAREVGSFPIRTLTLDEGDRRPLGVGAGSLALLAHLPDDEIEVIIARNRRW
                     LQDYAGFSPDELLRLVARTRRDGFALNEGRIVPAMQAIGVAVVTRSGRPVASLSLAAI
                     RDRMGADRLPKLVHRLREEAEALAEMLDPPPISRDVQPVAGA"
     gene            1067..1864
                     /locus_tag="VFX46_06615"
     CDS             1067..1864
                     /locus_tag="VFX46_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600287.1"
                     /translation="MIEAPTATKFPPEARGNVVVCGSHGGRYPAYLAAQAGLRAVIFC
                     DAGVGRDGAGIESLSLLGQGGMAAATVSHMTARIGEPADMLARGIISHVNEPARAVGV
                     EPGMPCRLAAERLEAAEVRTVHIPPSEEARTVLTLPGAARRLVLIDSAAMVEPNDSGQ
                     IIVTGSHGGLVGANPAYALRVDGFAAVFNDAGIGIDEAGIARLPALQERGIAAFTVAA
                     ESARIGDARSTYEDGVISAVNERARELGAIPGKRAKDVLLAWARGVT"
     gene            1925..>2673
                     /locus_tag="VFX46_06620"
     CDS             1925..>2673
                     /locus_tag="VFX46_06620"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEX5600288.1"
                     /translation="MRKPVWCGIVFLFFALAVAPWAAAETLTLKLVHNHAPGTIWQEV
                     AEDYASRVHQRSGGKVKIEVVHADANGVALTAEDLLAGDHDIMMHPVGALSRYDALAG
                     LEAYPYLIRDIAHFRAVYDGALGKELLTEIGKRTAFHLIGAAYRGTHHLSSNRLVEKI
                     DDLRDLKVRVPELKIFELTWEYLGASPVPMDFSELYEALREEDVMAQESPLKTMRDAN
                     LSEVQKYVIETGHILGAMTFILPDDRYWALP"
BASE COUNT          524 a          827 c          814 g          508 t
ORIGIN      
        1 cccagcacca ccttctcttc gaggccgcgg agggcttgct cttcgaatgc gcggatgcgg
       61 tgcatcaggc gatgatgcgc acgcagccgc tcaacatcga tgttgcgacg taagctcata
      121 atgcgcgtca ggctccggca acaggttgaa cgtctcggct tattggaggc gggtcgagca
      181 tctcggcgag cgcttccgcc tcttcgcgca gccgatgaac gagctttggc aggcggtcag
      241 cgcccatccg atcccggatg gctgcgaggc tcaaagacgc gacagggcgt ccgctgcggg
      301 tcaccaccgc cacgcctatg gcttgcatcg caggcacgat acgcccttca ttgagcgcga
      361 agccgtctct tcgcgtgcgc gcaacgagcc gcagtagctc gtcaggcgag aatcctgcat
      421 agtcctgcaa ccaccgcctg ttgcgcgcga tgatgacctc aatctcatcg tcgggaaggt
      481 gcgcgagcag cgcgaggctt cccgccccta ctccgagcgg acgtcggtca ccctcatcaa
      541 gcgtcaatgt gcgaatgggg aagctgccaa cctcgcgggc gatgcagacg gcggcaaggc
      601 cctcgcgcac ggaggcgaac accgtatcct ccgtctcggc ggcgagccgc cgcaacacag
      661 gccggacgag acttccgatg tgatggattg cagcgtcacg ccccagggcg gcagcagagg
      721 cgccgagcct gtaacgtttc gttttcagat cctggtggac gaagccaaca ttgaccaaag
      781 cgccaagcaa tcggtgcgcc gtagtcttac caaggccggt cgtctccgcg atgtcactca
      841 aacggaatcc gccgtcaccc gcggcagaaa gcagacgcag gatcgtcgcg acccgatcca
      901 cagtcttcac tgtgggttcc tggttcaaag ccacacctcg tccgcaatct ctgcccgcaa
      961 cctcttccaa attagcggaa cttggatagc atgtttttgg aacacaggtc attcgttttc
     1021 acaacgtccc acacggttgg ccagaaaggc cagagagaca cgcgccgtga tcgaagcgcc
     1081 gacggcaaca aaatttcctc ctgaagcgcg cggaaacgtt gtcgtctgcg gaagtcacgg
     1141 cggccgctat cccgcctacc tcgccgcgca agctggtctc cgggctgtca tcttctgcga
     1201 cgccggcgtt ggccgcgacg gtgcgggaat cgagtccctc tcgctcctcg gccaaggcgg
     1261 catggccgcg gcaaccgtct cccacatgac cgcccgcatt ggggagccgg cggacatgct
     1321 cgcgcgcggc atcatttctc acgtcaacga gccggcgcga gcggtcggcg tcgagcccgg
     1381 catgccatgc cggctggcag cggagcggct agaggctgcc gaggtccgca ccgttcatat
     1441 cccgccatcg gaggaagccc gcacggttct cacccttccc ggcgcagccc gccggctcgt
     1501 gctgatcgac tccgctgcca tggtggagcc caatgattct ggtcagatca tcgtgaccgg
     1561 ctcccacggg gggctggttg gcgccaatcc ggcatatgct ctccgcgtcg acggatttgc
     1621 cgccgtcttc aacgacgccg ggatcggcat agacgaggca gggatcgccc gtttaccggc
     1681 cctccaagaa agaggcattg cggcttttac agtcgccgct gagagtgcca ggattgggga
     1741 tgcgagatcg acgtacgagg acggcgtcat ttctgccgtg aacgaaagag ctcgtgagct
     1801 gggtgccatc ccgggtaagc gggcgaagga cgtgctgctc gcctgggcgc ggggtgtcac
     1861 atgaggtagg atcattggcg gtgcgcgccg ccgcagcagc ggttgaggcc agaagaatag
     1921 gaaaatgaga aaacctgtct ggtgcggaat tgtattttta tttttcgcat tggctgtggc
     1981 gccgtgggcc gcggcggaaa ccttaacgtt gaaactcgtt cacaaccacg caccagggac
     2041 gatctggcag gaggtcgccg aggattacgc gtcgcgagtc catcagcgct ccggcggaaa
     2101 ggtaaagatc gaggtcgtcc acgcggacgc taacggcgta gccctcacag ctgaggatct
     2161 ccttgccggc gaccatgaca tcatgatgca tcccgtgggg gcgctttctc gatacgacgc
     2221 tctcgctggg cttgaagctt atccttatct cattcgcgat attgcgcatt tcagagccgt
     2281 ttacgacggt gctctcggca aggagcttct gactgaaatc ggaaaaagaa cggcttttca
     2341 ccttatcggc gcggcttatc gcggaactca ccatttgagc tccaatcgtc tggtcgagaa
     2401 aatcgacgat ctccgcgatc tcaaggtccg tgtaccggag ctgaaaatct tcgagctcac
     2461 ctgggaatat ttaggcgcct ctcccgtgcc aatggatttc tctgaacttt acgaggcgtt
     2521 gcgcgaggaa gatgtgatgg cccaggaaag cccgctgaag acgatgcggg atgcaaacct
     2581 ttcagaggtg cagaagtacg tcatcgagac cggacacatc ctcggcgcca tgacgttcat
     2641 ccttcccgat gatcgctatt gggctctgcc aga
//
LOCUS       DASUMG010000401         5512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_694640, whole genome shotgun sequence.
ACCESSION   DASUMG010000401 DASUMG010000000
VERSION     DASUMG010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694640"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFX46_06625"
     CDS             complement(<1..563)
                     /locus_tag="VFX46_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600289.1"
                     /translation="MLTLENIEVVYDQVFLAIKGVSLEVAEGGAVALLGSNGAGKSTT
                     LKAISGLLAPERGKVTRGEIRFRGQDLLGLSAPERVRLGIAHVLEGRHVFGHLTPLEN
                     LIAATSMHRSRAKINELTERVFEWFPRLKQRSNAQAGYLSGGEQQMLAIGRALMTEPK
                     LLMLDEPSLGLAPFLVDEIFEIISRIVR"
     gene            complement(583..1824)
                     /locus_tag="VFX46_06630"
     CDS             complement(583..1824)
                     /locus_tag="VFX46_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600290.1"
                     /translation="MRRLTTALAGAAALALASGPGWAQEKVIKFGFAQDFTKVYTFVT
                     SEYSQGQRDYLTLINERGGINGYKFDVTVVDHGNEPQRGIEAYERFKADGAILVDFFS
                     TPVSRAVVPRALEDGINVLTMFHGRSDAADGETFPTVFPAGALYWSQAAVILKYIADQ
                     EGGNLKGKKIALVGIDSPFGKEPVPVFEAVRDKEGFELQTFFYPSPGNEQSSTWTAVR
                     RYGPDWVVIWGAGGGQTVSLRDAIRNGIKLDRLLSVIWLSETDTEIVGKDAAKGVLRF
                     EGAAYGRDNAVIKAILDEVYAKGKGAGDEKKVGSSYYNVGVAGSAIVVEATRIALEKF
                     GEPMTAEKLKKGMEEIRDFNAEGLIPPLTITAQDHQGGGRGRISQWDGEHWVPKTDWY
                     AAYQDVVMDLVKKTAAEFKKN"
     gene            complement(1840..2916)
                     /locus_tag="VFX46_06635"
     CDS             complement(1840..2916)
                     /locus_tag="VFX46_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5600291.1"
                     /translation="MFYRLAGVRHTTYRSARRLYPVPFDRHLLTAITVIAIAAPFLFS
                     PLALNSYLLPWIIWSTAALGLNLLMGWAGQIHLGYAAVMAIGAYTSIHSVRAGVPFEI
                     SLILAGLAAAAIGFIFGAAALRVKGLYLAVTTLAMQSIVDWTIVHVPAISGGSQATLQ
                     APPLTVLGWPITSDAGRYWFALATCAVVTTFLLNVNRTSLGRALVAVREKDFAAAIIG
                     VNPFKYKLLAFWCSSFIGGVTGAVLAFCYYRAVTPEQFHLEVSIQAVAMVIVGGLGSV
                     LGSFFGAGLVLLAPIFLNNLVSAVAAALNLPLYADIISHIPLVLYGALIVGFLLYEPL
                     GLAKIYDNVRNYFLVWPFRHTRHG"
     gene            complement(2916..3806)
                     /locus_tag="VFX46_06640"
     CDS             complement(2916..3806)
                     /locus_tag="VFX46_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5600292.1"
                     /translation="MDLLYELAELSINGALVGLMYGLVALGIVLVFKASSVANLAQGA
                     LVMVGAYLTWAATAQLGTPLWAAIPIGIVAMYFVGRGIERVALRRMIGQPVIMVIMLT
                     LGLEIMLRGLLPAIFGATKKPLDIGLDTAPIFLGPILIDRSYLLTGIVALALVGLAAL
                     FFGTRQGIMLRAVSDDQTASWSVGIPVERAIGISWGLAGVAAAAAGILWGSIQGVDWT
                     LSLLLIKALAVAILGGLDSIAGVIVAGLVVGILENVAPVYLERIGISGTRDVVASLVI
                     LATVIVRPYGLFGREDIERV"
     gene            complement(3812..>5512)
                     /locus_tag="VFX46_06645"
     CDS             complement(3812..>5512)
                     /locus_tag="VFX46_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5600293.1"
                     /translation="GDNRPRLYFGMLSAAALRGYPAPVYPDVPPDDLEYYAGNSRARF
                     ALAEDQEQVDKLLELRKRARLPEFIIYDDPRGLWNYTEPGLLSWEDLIREGAERLARE
                     PGLKEDLINRAKPTDAAVLLHSSGTTGKPKGVVLQHRRVIAAARSAEQADYFRAGDEL
                     MAYLPMAWVGDFVFTVSSGIALRFTINIPERQETVLHNLREVAPTVYFASPRSWDAML
                     THVQVGIAESTWLKRKLFNWFMPFAIRLERERLAGRKPGLLSRIGYAIGDLLMFAPLR
                     DYLGLSRAQRAYTAGEAIGEDTFLFFRALGLDLKQFYGQTENCALTAAQSSGDVKLNT
                     VGKPLPGVEIKIDDNGEILIRADSVFDGYLNDEEATRKTLAGGWLHTGDAGYLDDDGH
                     LVVLGRVGEVVYTKGGERYIPTYIENRLKFSQYVKDAAIIGAGRDYLTALICIDMGAV
                     GHWAEMNGVTYTSYAELSQRPEVYELIAGVVRHVNELLPPALAIRKFVNLPKEFDPDD
                     GEVTRTRKLRRNVIDEHYAPIIAALYDGSESVEHETLITYETGATGFLKRRLAIFTVN
                     "
BASE COUNT         1107 a         1690 c         1721 g          994 t
ORIGIN      
        1 cgcacgatcc gggaaatgat ttcgaaaatt tcatcgacga gaaacggagc caagccgagg
       61 ctcggctcgt ccagcatcag cagctttggc tcggtcatca aagcacggcc gatggcgagc
      121 atttgctgct cgccgccgga aagatagccg gcctgagcat tagagcgctg cttcaagcgc
      181 ggaaaccact cgaatacgcg ctcggtcaat tcgttgatct tggcccggct gcggtgcatt
      241 gacgtcgcgg cgatcaaatt ctccagcggc gtcaggtggc cgaagacgtg acggccttcg
      301 agcacgtggg cgatgccgag ccggacccgc tccggggccg aaagccccag caggtcctgg
      361 ccgcggaagc ggatctcgcc gcgggtcacc tttccgcgct ccggcgccag caggccggaa
      421 atggccttga gcgtcgtgct cttgccggcg ccgttcgagc cgagcagcgc gaccgcgcct
      481 ccctcggcga cctcgagcga gacgcccttg atcgcgagga agacctgatc gtacacgacc
      541 tcgatgtttt cgagtgtcag cattgatgga cgaatggcag cctcagttct tcttgaactc
      601 ggcggcagtc ttcttcacga gatccatcac gacgtcctgg taagctgcgt accagtcagt
      661 cttcggcacc cagtgctcgc catcccactg cgagatgcga ccacggccac cgccttggtg
      721 gtcctgcgcc gtaatggtga gcggcgggat cagaccttcg gcattgaagt ctctgatttc
      781 ctccattcct ttcttcagct tctcagccgt catcggctct ccgaatttct cgagtgcaat
      841 gcgagtagcc tcgaccacga ttgctgaccc ggcgacgccg acgttgtagt agctgcttcc
      901 gacttttttc tcgtcaccgg cgcctttgcc cttggcgtag acttcgtcga gaatggcttt
      961 gatgacagcg ttatcacgcc cataggcggc gccttcgaag cgcagcacgc ccttggccgc
     1021 atccttgccg acgatttcgg tatccgtttc ggagagccaa atcacggaca ggagccggtc
     1081 gagcttgatc ccgttgcgga tggcatcacg cagcgagacg gtctggccac caccggcacc
     1141 ccagatcacg acccaatccg gaccgtagcg gcgaacagcg gtccaagtcg aagactgctc
     1201 gttacccgga gacgggtaga agaatgtctg cagctcgaat ccttccttgt cgcggacggc
     1261 ctcgaaaact gggacaggtt ctttgccgaa gggcgagtcg atgccgacca gagcaatctt
     1321 cttgcctttc aggtttccgc cttcctgatc agcgatgtat ttcaggatta ccgcggcttg
     1381 cgaccaataa agagcaccgg ccggaaatac ggtcgggaac gtctcaccat ctgccgcatc
     1441 ggagcggccg tggaacatgg tgagcacgtt gatcccatcc tccagcgcac gcggcacgac
     1501 cgcacgggaa accggggttg agaagaagtc tacgagaatc gcgccatcgg ctttgaagcg
     1561 ctcgtaggcc tcgatgccgc gctgcggctc gttcccgtga tcgacgacgg tgacgtcgaa
     1621 tttgtacccg ttgatgccgc cccgctcatt tatcagggtg agatagtcgc gttggccctg
     1681 gctgtattcc gaggtcacga acgtgtagac cttcgtgaaa tcttgggcga agccaaattt
     1741 gatgactttt tcctgcgccc atcccgggcc ggacgccagc gcgagcgccg cggcgcctgc
     1801 gagtgcggtc gtcaatctcc tcatcgtttc ctccattacc taaccatgtc gcgtgtggcg
     1861 gaagggccaa acgagaaagt aattgcgcac gttgtcgtaa atcttggcaa ggccgagcgg
     1921 ctcataaagc agaaagccga cgatcagagc gccataaagc acgagcggaa tatgcgaaat
     1981 gatgtccgca tagaggggca ggttgagcgc agccgctacc gcactgacga gattgttgag
     2041 aaaaatcggc gccaataaca cgaggcccgc gccgaagaag ctgccgagta cgctgccaag
     2101 gccgcccaca atcaccattg cgaccgcctg gatcgagact tccaggtgga attgctcggg
     2161 cgtgacagcg cgataatagc agaaggccag caccgcacct gtcacgccgc caatgaacga
     2221 tgagcaccag aaggcgagca atttgtattt gaatggattg acgccgataa tcgccgcagc
     2281 gaaatccttt tcccgcaccg cgaccagcgc ccgtcctaga ctcgtccggt tcacattgag
     2341 cagaaaggtg gtcaccaccg cacaggtagc cagcgcaaac cagtagcggc cagcatccga
     2401 cgtgatgggc caccccaaca ctgtcagcgg tggggcttgc aacgtcgctt ggctgccgcc
     2461 gctgattgcc ggcacgtgca cgatcgtcca gtcgacgatt gattgcatgg cgagcgtagt
     2521 cactgcgaga tagagacctt tcacccgcag cgccgctgcg ccgaagatga aaccgatagc
     2581 cgcggccgcg agaccggcaa gaatgagaga aatctcgaat ggcacacctg cgcgcacgct
     2641 gtgaatcgag gtgtacgcgc cgatggccat cacggcggca tagccaaggt gaatctgtcc
     2701 agcccagccc atcagcaggt tgaggccgag cgccgccgtc gaccagataa tccagggaag
     2761 gaggtagcta ttcagcgcga gcggcgagaa tagaaatggc gccgcgatgg caatgaccgt
     2821 gatcgctgtc agaagatgcc ggtcgaacgg caccggatag agccgccgcg cggaacggta
     2881 agtggtgtga cggacgccag ccagtctgta gaacatcaca cgcgctcaat atcttcgcga
     2941 ccgaacaagc cgtagggccg gacgatgaca gttgccagga tgacgagcga ggcgacgacg
     3001 tcccgcgttc cggaaattcc gatgcgctcc agatagacgg gcgcgacgtt ctccagaatg
     3061 ccgaccacga ggccggcgac gatgacgccg gcgatgctgt cgagcccacc caggatggca
     3121 actgccagag ctttgatgag cagcagggac agcgtccagt cgacgccctg gatggagccc
     3181 cagaggatgc ctgccgcggc ggctgcgacg ccagcgaggc cccacgagat gcctatcgcc
     3241 cgctcgacgg ggatacccac cgaccacgag gctgtctgat catccgagac agcccgtagc
     3301 atgatgccct gccgcgtgcc gaagaacagc gccgcgaggc cgaccagcgc cagcgcgacg
     3361 atacctgtca gcagatacga gcggtcgatg agaatgggac cgaggaagat cggtgcggtg
     3421 tccaggccaa tgtccagtgg cttcttcgtt gcaccgaaaa tggcgggcag cagaccgcgc
     3481 agcatgatct cgagcccgag cgtcagcata atgaccatga tgaccggttg cccgatcatg
     3541 cggcgtagcg cgacccgttc gatgccgcgc ccgacgaagt acatcgcgac gatgccgatg
     3601 gggatggcgg cccagagcgg ggtgccgagc tgggcggtgg ccgcccaggt gaggtacgct
     3661 ccaaccatca cgagggcgcc ttgggccagg ttggcgacgc tggacgcctt gaacacaagc
     3721 acgatgccaa gggccacgag cccgtacatc agcccaacca acgcgccgtt gatggataac
     3781 tcggccagtt cgtagaggag atccatgacg ttcagttcac cgtaaagatg gcgaggcggc
     3841 gcttgaggaa accagttgcg ccggtctcgt acgtgatgag ggtctcgtgc tcgacggatt
     3901 cggatccgtc gtagagggcg gcgatgatcg gcgcgtaatg ctcgtcaatg acgttgcggc
     3961 gcagcttgcg ggtgcgggtc acctcgccgt cgtcgggatc gaactctttc ggcagattca
     4021 cgaatttgcg gatcgcgagc gccggcggca gcagctcgtt gacgtgccgc acaacgccag
     4081 cgatgagctc gtagacctcg gggcgctgtg aaagctcggc gtacgacgtg taggtcacgc
     4141 cgttcatttc ggcccagtgg ccgacggcac ccatgtcgat gcaaatgagc gccgtcaggt
     4201 aatcgcggcc ggcgccaatg atggccgcgt ccttgacgta ctggctgaac ttcagccggt
     4261 tctcgatgta ggtggggata taacgctcgc cgcccttggt gtagacgacc tcgcccacgc
     4321 gccccagcac gacgaggtgg ccgtcatcgt ctaggtagcc ggcatcgccc gtgtgcaacc
     4381 atccgcccgc caatgtcttt cgcgtggctt cctcgtcatt gaggtacccg tcaaacacgc
     4441 tgtctgcgcg gataaggatt tcaccgttgt cgtcgatctt aatctcgact cccggcagcg
     4501 gcttgccgac ggtgttgagc ttcacatcgc cggagctttg cgcggccgta agcgcgcaat
     4561 tctcggtctg gccatagaac tgcttcaggt cgagcccgag cgcacggaag aagaggaaag
     4621 tatcctcgcc aatggcttca cccgccgtgt aggcgcgctg ggcacgggaa aggccgaggt
     4681 aatcgcgcag gggcgcgaac atcagaagat cgccgatggc atagccgatc cgggagagga
     4741 gccctggctt ccggccggcg agtcgttccc gctccagtcg gattgcgaac ggcatgaacc
     4801 agttgaacag cttccgcttc agccaggtcg actcggcgat gccgacctgc acatgcgtga
     4861 gcatcgcgtc ccaacttcgt ggcgacgcga agtacacggt cggggccacc tcgcggagat
     4921 tgtgaagaac ggtttcctgg cgctcgggga tgttgatcgt gaatcgcagc gcgatgccgg
     4981 aggagacggt gaacacgaag tcgccaaccc aggccattgg gagataggcc atcagctcat
     5041 cgccggccct gaagtaatcc gcctgctccg cactgcgcgc agctgcaatt acccggcgat
     5101 gctgcagcac aactcccttc ggcttccccg tcgtgccgga ggaatgaagc agcaccgcgg
     5161 catccgtcgg cttggcccgg ttgatgaggt cctctttgag ccccggctcg cgggcgaggc
     5221 gttctgcgcc ttcgcggatt aggtcctccc aggagagcag gccgggctcc gtgtagttcc
     5281 aaagcccgcg cgggtcgtcg tagatgatga actcgggcag tctggcccgc ttccgcaact
     5341 cgagcagctt gtcgacttgc tcttggtctt ccgcgagcgc gaaccgtgca cggctgttgc
     5401 cggcgtagta ttcgagatcg tcaggcggta catccgggta gactggtgcc gggtagccgc
     5461 gcagggccgc ggcgctcagc atgccgaaat agagccgtgg gcgattgtcg cc
//
LOCUS       DASUMG010000402         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_52631, whole genome shotgun sequence.
ACCESSION   DASUMG010000402 DASUMG010000000
VERSION     DASUMG010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52631"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="VFX46_06650"
     CDS             <1..996
                     /locus_tag="VFX46_06650"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX5600294.1"
                     /translation="ERRNEVVSFVKGGLEDLSISRTTLDWGIPVPGNPKHVMYVWVDA
                     LTNYITGVGFPDENHPNWHYWPADVHVIGKDILRFHAVYWPAFLMSAGLPLPRRIFAH
                     GFLFNRGEKMSKSVGNVIDPFDLVKAYGVDPLRYFFLREVVFGQDGSYSPEAIVNRIN
                     ADLANDLGNLAQRSLSMIYKNCGAAIPEPGAFSDADKAILDATDALYGQARAAMDRQA
                     IKQYLDAVWAVVGETNRYFASEEPWAKRKTDFKRMETILYVTAEVVRQVAILAQAVMP
                     SSAAKLLDLLGQGPDVRSFAALGPSGRLKPGTPIPQPTAVFPRYVESEEAEKGKG"
     gene            996..1796
                     /locus_tag="VFX46_06655"
     CDS             996..1796
                     /locus_tag="VFX46_06655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX5600295.1"
                     /translation="MLVDHHCHLDFPQFAKDRDGVVARAARAGVGMLVTISTRIRQLP
                     DLIALAERYPNVFCSVGTHPHYAHEELDVSVDEIVRLAEHPKVVAIGEAGLDYFYKRS
                     PPEAQAEGFRRHIAAARITGLPLEIHTREADEDTARILEEEHAKGPFPAILHCFTGGR
                     DLAMRALALGLTISFTGVITFRKSEELRAIAAEVPLDRLLVETDAPFLAPEPYRGKLN
                     EPAYVVHTAEMLARVKGVTPQELAEATTENFFRLFKKVPRAASAADAA"
     gene            1793..>2386
                     /locus_tag="VFX46_06660"
     CDS             1793..>2386
                     /locus_tag="VFX46_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5600296.1"
                     /translation="MRHRLTILGCGSSGGVPRLGNDWGRCDPNNPKNRRKRCAALIEQ
                     IGPEGTTKVLIDTGPDIREQLLSVNVTSLDGVLYTHDHADHTHGIDDLRPVAYAMKRR
                     VPVWFDARTRDSLVTRFAYCFETPPGGSYPPILEAKDINPPEAVRIPGAGGVMEVIPI
                     VQEHGDITSLGFRIGGLAYSPDISGLPESSIPLLQNLD"
BASE COUNT          426 a          762 c          757 g          441 t
ORIGIN      
        1 gagcggcgca acgaggtcgt gagctttgtg aaaggcgggc tcgaggacct atccatttcg
       61 cgcacgacgc tcgactgggg cattcccgtt cctgggaacc cgaagcacgt gatgtacgtg
      121 tgggtcgacg cgctcaccaa ctacatcacc ggtgtcggct tccctgacga gaaccacccg
      181 aactggcact actggccggc cgacgtgcac gtgatcggca aggatatcct gcgctttcat
      241 gccgtgtact ggcccgcgtt cctgatgtcg gcaggccttc cgttgccgcg ccggatcttc
      301 gcccacgggt tcctgttcaa ccgcggggag aagatgtcga agtcggtcgg caacgtgatc
      361 gatcccttcg atctcgtgaa ggcctatggc gtcgatccgt tgcgctactt cttcctgcgg
      421 gaggtcgtct tcggacagga tggcagctac agcccggaag ccatcgtcaa ccgcatcaat
      481 gcggatctgg ccaatgacct cggcaacctg gcccagcgct cgttgtcgat gatctacaag
      541 aactgcgggg cggccatccc cgagccggga gctttcagcg acgcggacaa ggcgatcctc
      601 gatgcgacgg acgcgctcta cggccaggcg cgcgcggcta tggatcggca ggcgatcaag
      661 cagtatctcg atgcggtgtg ggccgtggtg ggggagacca atcgctactt cgccagcgag
      721 gagccttggg ccaagcgcaa gaccgatttt aagcgcatgg aaacgatcct ctatgtgacc
      781 gcggaggtcg tgcggcaggt cgcgatcctt gcacaggcgg tgatgccaag ctcggcggcg
      841 aagctgctcg atctgctcgg ccaaggtcct gatgtgcgga gcttcgcggc tctggggccc
      901 agcggccgct tgaagcctgg cacccccatt ccacagccga ccgcagtatt cccgcgttac
      961 gtcgagtccg aggaggcgga aaagggcaag ggctgatgct ggttgatcac cactgccacc
     1021 ttgactttcc gcagttcgcg aaggatcggg acggggtcgt cgcccgcgcc gcccgggccg
     1081 gcgtcgggat gctcgtcacg atatcgaccc gtatccgtca gctgccggac cttatcgcgc
     1141 tcgccgagcg gtatcccaac gtcttttgtt cagtcggcac gcatccgcat tacgcccatg
     1201 aggagctcga tgtctccgtg gacgagatcg tgcggctggc ggaacacccc aaggtggtgg
     1261 cgattggcga ggcagggctg gattacttct acaagcgaag cccgccggag gctcaggcag
     1321 agggcttccg gcgccacatc gcggcggccc ggatcaccgg ccttccgctg gagattcaca
     1381 cgcgcgaagc cgatgaggac accgcgcgaa tcctcgagga ggagcacgcg aagggtcctt
     1441 ttccggccat cctgcattgc ttcaccggtg ggcgcgatct cgcgatgcga gctctcgcgc
     1501 tcggcctgac gatttccttc accggcgtca ttacgttccg aaagtcggag gagctgcggg
     1561 ccatcgcagc ggaagtgccg cttgatcgcc tgctggtgga aacggatgcg cctttcctcg
     1621 cgcccgagcc gtaccgcggc aagttgaacg agcccgccta cgtggtgcac acggccgaga
     1681 tgctcgctcg cgtcaaaggc gtcaccccgc aggagctggc cgaagcgacc accgagaact
     1741 tcttccggct gttcaagaag gtgccgcgcg cagcgtcggc agctgatgcc gcatgaggca
     1801 ccgcctgacg atccttggct gcggttcgtc cggcggcgtc ccgcgcctcg gcaacgattg
     1861 gggtcgctgc gatccgaaca atcccaaaaa ccggcgtaag cggtgtgcgg ctctcatcga
     1921 gcagatcggc cccgagggca caacgaaagt gctcatcgac acggggccag acattcgtga
     1981 gcagctgctt tcggtcaacg tcacgtcgct cgatggcgtg ctttatacgc acgatcacgc
     2041 cgatcatacg cacggcatcg acgatttgag accggtcgcc tacgcgatga aacggcgggt
     2101 gccggtgtgg ttcgacgcgc gcacccgtga cagcctcgtg acccgcttcg cttattgctt
     2161 cgagacgcct cccggcggca gctatccgcc catcctcgag gccaaagaca tcaatccacc
     2221 cgaagccgtg cgaatccctg gagcgggtgg cgtgatggag gtcatcccta tcgtgcagga
     2281 gcacggcgat attacatctc tgggcttccg catcggcggt ctagcttatt cgccagacat
     2341 cagcggcctg cctgaatctt ccattccgct cctgcagaac ctggac
//
LOCUS       DASUMG010000403         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_373929, whole genome shotgun sequence.
ACCESSION   DASUMG010000403 DASUMG010000000
VERSION     DASUMG010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373929"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..2272
                     /gene="parC"
                     /locus_tag="VFX46_06665"
     CDS             20..2272
                     /gene="parC"
                     /locus_tag="VFX46_06665"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX5600297.1"
                     /translation="MTSKPILPGPGQIEPINLKDALEERYLSYALSTIMGRALPDVRD
                     GLKPVHRRILYAMRELGLNPDAAFKKCARVVGDVIGKYHPHGDQAVYDALVRLAQDFA
                     QRYPLIEGQGNFGSIDGDNAAAYRYTEARLSETALRLLEGIDEETVDFRPTFNGEDKE
                     PVVLPANFPNVLANGSSGIAVGMATNIPPHNVEELCNALLHLIKHPNASIEKLVEFIP
                     GPDFPTGGVLVEPRESIIEAYKTGRGSFRLRAKWHREDTGRGTYQVVITEIPYQVPKS
                     KLVERIAELLQERKLPLVGDVRDESTEEVRLVIEPKSRSVDPVLMMESLFRLTELEIR
                     FGLNMNVLSHGQIPNVLSLRDVLRQWLDHRKEVLIRRSQFRLRKIEHRLEVLDGYLIA
                     YLNIDEVIRIIRFEDDPKAKLIARFGLTEVQAEAILNLRLRALSKLEEIEIKAEHNRL
                     SKEKRELQQLLKSEDLQWARIADEVRETRERYSKKTELGRRRTSFAEPPEIEVDLDQA
                     MVEKEPITVILSEKGWIRAMKGHLDDVSKLDFKQGDRLKRAVKAFTTDKLLVFATNGR
                     FFTLSGNELPGGRGHGEPIRLMIDLEESHDVLELFVHQPGRKLLVAATSGHGFIVPED
                     EVVAYTRKGRQVLNVSEPDEAKVCVPAEGDMVATLGDNRKMLVFPLEEVNEMVRGKGV
                     ILQRIKGGKLADARVFSKEAGLTWQDAAGRTYTLSMKELSEWVGQRAQAGKIAPKGFP
                     RSNRFGPAFS"
     gene            2404..2823
                     /locus_tag="VFX46_06670"
     CDS             2404..2823
                     /locus_tag="VFX46_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600298.1"
                     /translation="MILMAASISTVADAQDTCTRADFEAVVDDAAAALNELNAKHKPT
                     FQEYLRQLKEKNGWDHDTFMKEALPYVRDEQIAKYNQETGDYLARISSLGEEGSTAVK
                     PDCKLLDQVKEAMEGLVAVQRAKWNYMFEKIGRALAP"
BASE COUNT          627 a          901 c          938 g          516 t
ORIGIN      
        1 gctcagttag tcaccgtaca tgacaagcaa accgattctg cccgggcccg gccaaatcga
       61 gcctataaac ctcaaggacg cgctcgaaga gcgctacctc tcctacgcgc tgtccaccat
      121 catgggccgc gcgctcccgg atgtgcgcga cggcctgaag cccgtacacc ggcgcatcct
      181 ctacgcgatg cgcgagcttg ggctcaaccc ggacgccgct ttcaagaagt gcgcccgcgt
      241 cgtcggcgac gtcatcggta agtatcaccc ccacggcgat caggccgtct acgacgcgct
      301 ggtgcgcctc gcgcaggact tcgctcagcg gtaccccctg atcgaaggcc aggggaactt
      361 tggcagcatc gacggggata acgccgccgc ctatcgctac acggaggcgc ggctttccga
      421 gactgccctc cggctcctcg aagggatcga cgaagagacg gtcgacttcc gtccgacctt
      481 caacggcgag gacaaagagc cggttgttct acccgcgaac ttcccaaacg tgctcgccaa
      541 cggctcgtcg ggcatcgcgg tgggcatggc gaccaacatc ccgccgcata acgtggagga
      601 gctgtgcaac gcgctcctgc accttatcaa gcacccgaac gcctcgatcg aaaagctggt
      661 cgagttcatt ccggggccgg acttcccgac gggcggcgtg cttgtcgagc cgcgcgaatc
      721 catcatcgag gcctataaga ccggtcgcgg cagcttccgc ctgcgcgcga aatggcatcg
      781 tgaggatacc ggtcgcggca cctaccaggt cgtgatcacg gagatcccct atcaggtgcc
      841 gaagtccaag ctcgtcgaaa ggatcgccga gctgctgcag gagcgtaagc tgccgctggt
      901 gggcgacgtc cgcgacgagt cgacagagga ggtgcgcctc gtcatcgagc cgaagagccg
      961 gagcgtcgac cccgttttga tgatggaaag cctgttccgg ctgaccgagc tcgagatccg
     1021 cttcggcctc aacatgaacg tgctgtcgca cggccagatc ccgaacgtgc tgtcgttacg
     1081 ggacgtgctg cggcagtggc tggatcaccg caaggaggtg ctgatccggc gctcgcaatt
     1141 ccggctgcgg aagatcgagc accggctgga agtcctcgat ggctatctga tcgcctacct
     1201 caacatcgat gaggtgatcc gtatcatccg tttcgaggat gatccgaaag cgaagcttat
     1261 tgcccggttc gggctcaccg aggtccaggc cgaggccatc ctgaacctca ggctgcgggc
     1321 gctgtctaag ctcgaagaaa tcgagatcaa agccgagcac aatcggctct cgaaagaaaa
     1381 gcgcgagcta cagcagctgc tcaaatccga ggatctgcag tgggcgcgga ttgccgacga
     1441 ggtgcgcgag acccgcgagc gctactcgaa gaaaaccgag cttggccgcc gccgcacgtc
     1501 gtttgctgag ccgccggaaa tcgaggtcga cctcgaccag gcgatggtcg agaaggagcc
     1561 gatcacggtc attctctccg agaagggctg gatccgcgcg atgaaggggc atctcgacga
     1621 tgtctcgaag ctcgacttca agcagggtga tcggctgaag cgcgcggtga aagctttcac
     1681 gaccgacaag ctgctcgttt tcgcgaccaa cggcaggttc ttcacgctct cgggcaacga
     1741 gctgccggga ggccgcggtc acggcgagcc gatccgcctg atgatcgacc ttgaggaaag
     1801 ccacgacgtg ctcgagttgt tcgtgcacca acccggccgc aagctgctgg ttgcggccac
     1861 cagcgggcac gggttcatcg tgccggagga cgaggtcgtg gcgtacaccc gcaagggccg
     1921 gcaagtgctc aacgtcagcg agccggacga ggccaaggta tgcgtcccgg ccgaaggcga
     1981 tatggtcgcg accctgggcg acaaccgcaa gatgctcgtg ttcccgctcg aagaggttaa
     2041 cgagatggtc cggggcaagg gcgtgatcct gcagcggatc aaaggcggca agctcgcgga
     2101 tgcgcgcgtt ttcagcaagg aagccgggct cacttggcag gatgcggctg gacgcaccta
     2161 cacgctcagc atgaaggagc tcagcgagtg ggtcggccag cgggcccaag ccggaaagat
     2221 tgcacccaaa ggttttccgc ggtcgaacag gttcgggcca gcttttagct agatgaagac
     2281 tggctcgctt aaggtcattg cggccgcgtg ctgaggaaat acttgcgctt cgctggaggg
     2341 tgggccatgg gtaggctgca cgcggacggc gggaacgatc agccaatgtg gcgttcggtt
     2401 gcaatgatcc tgatggcggc ctccatcagc acggtcgcag acgcgcaaga tacctgcacc
     2461 agggcggatt ttgaggctgt cgttgacgat gcggctgcgg ctctgaacga gctcaacgcc
     2521 aagcacaagc ccacatttca agaatacctg cgccagctca aagaaaagaa cggctgggac
     2581 catgacacct ttatgaaaga agcgcttccg tacgtccgcg acgagcaaat tgccaagtac
     2641 aatcaggaaa cgggcgatta tctcgcccgc atcagcagcc tcggcgagga ggggtcaacg
     2701 gcagtcaaac cagattgcaa gttgcttgac caggtaaagg aggcgatgga gggcttggtc
     2761 gcggttcagc gggccaaatg gaactatatg ttcgagaaga tagggcgcgc gctcgcacct
     2821 taagcggcaa cgtctccccc tgccaaatct ctccgtccaa cgcgttagct atctaactgt
     2881 aagtgccatc taccggcagg ttgttcatgc caggcagtga gccggcggga tgcacgatcg
     2941 aaacttctgc caatgtctgc ttctgtgcat cggatcctca cc
//
LOCUS       DASUMG010000404         2198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_293802, whole genome shotgun sequence.
ACCESSION   DASUMG010000404 DASUMG010000000
VERSION     DASUMG010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293802"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /gene="pth"
                     /locus_tag="VFX46_06675"
     CDS             complement(<1..650)
                     /gene="pth"
                     /locus_tag="VFX46_06675"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX5600299.1"
                     /translation="MKLFVGLGNPGPKYARHRHNVGFMAVDRIAERHGFSPWRRRFQS
                     ETAEGTIGGERVLLLKPMTYMNESGRAVGEAARFLKIDLADIVVFHDELDLAPGKVRV
                     KTGGGNAGHNGLRSISAHVGNDFVRVRIGIGHPGSKEAVVPYVLHDFAKADQEWLDPL
                     LDNIADAAGRLAAGDTARFVTDIARAQQQADRGTSPAREVADRSHKPGSQPSEVGS"
     gene            826..1272
                     /locus_tag="VFX46_06680"
     CDS             826..1272
                     /locus_tag="VFX46_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX5600300.1"
                     /translation="MSTDTSADRQRWPRAAASAAIFRDDAVLLVKRSKPPVSGVWSLP
                     GGHIEPGERARDAALREVMEETGVTAEILGLADTHDVIIRDKKGMLKAHYLLAVFYGR
                     WLAGEPIAATDCADARFVPVEEVGSYPLTDGAQRIIEKAYGRLRNG"
     gene            complement(1343..>2198)
                     /locus_tag="VFX46_06685"
     CDS             complement(1343..>2198)
                     /locus_tag="VFX46_06685"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX5600301.1"
                     /translation="ITLLPIGGLASLERMPEKPSQEVFVALAGPAVNVVIAFILVVFA
                     GARLDPAALNLMENPASDMLSRIAIANVALVVFNLIPAFPMDGGRVLRALLAMRMGFV
                     RATRTAAVVGQSLAIFLGFIGLLGNPLLILIAVFVYLAATAESQYVEMRSLARGYLAR
                     DAMITKFEALTPTSTADDAVALLLRTTQQEFPVLDQFSRLQGLVTRDALIEVLRDRGG
                     STPVVEFMHKDVPIVAGTATLDNVVQMLQQKRARAVAVADPDNRLVGFITPENFAEMM
                     MVGRAREV"
BASE COUNT          437 a          716 c          672 g          373 t
ORIGIN      
        1 tccgacccca cctcgcttgg ctgcgaaccg ggcttatgcg atctatccgc cacttcacga
       61 gcaggggacg ttcctcgatc ggcctgctgc tgcgcacggg caatatcggt cacgaatcgc
      121 gctgtatcac ccgccgcaag ccggcccgcg gcgtcggcaa tattgtcgag aagcgggtcc
      181 agccattcct ggtcggcctt ggcgaaatcg tggagcacgt agggtacgac cgcctccttc
      241 gagccgggat gcccgatgcc gatccgcacg cggacgaaat cgttgccgac gtgagcactg
      301 atggaccgca agccattgtg gccggcattg cctccgccgg ttttcacccg aacttttccc
      361 ggcgccagat ccagctcgtc gtgaaagaca acgatgtcgg cgaggtcgat tttgagaaac
      421 ctcgctgcct cgcccacggc acggccgctc tcgttcatgt aggtcatggg cttgagcagc
      481 agcacgcgct cgccaccgat ggtgccttcc gccgtctcac tctggaaccg gcgtcgccac
      541 ggagaaaatc cgtgccgttc ggcaatgcga tccacggcca tgaagccgac gttgtgccgg
      601 tggcgggcat actttgggcc cggattaccg agtccgacaa aaagcttcat gacacgcgca
      661 cctcgctcgt cagagctctt ctgcgggcga tcgtggagga gctgcagagg gctggcagca
      721 tgttgaatct tgctttgaac ggcatgagca cgcttatcga tccactctcg acctgccgca
      781 agcccggatc taacgtggcc ctactatgat gagcaacggt gactcatgag cacggacaca
      841 agcgcggacc ggcagcgctg gccgcgcgcc gccgcgagcg cggccatatt ccgcgacgac
      901 gccgtactcc tggtaaagcg cagcaagccg ccagtcagcg gcgtatggag cttgcccggc
      961 gggcatatcg agccgggaga gcgcgcccgc gatgcggccc tgcgagaagt gatggaagaa
     1021 accggcgtaa cggcagaaat tctcggactg gccgacacac atgacgtaat catccgcgac
     1081 aagaagggca tgctcaaagc gcactatctc ctcgccgtgt tctacgggcg ttggcttgcg
     1141 ggcgagccca ttgctgcaac tgactgtgca gatgcccgct tcgtgcctgt cgaggaggtc
     1201 ggcagctacc ctttaactga cggcgctcag cgcatcattg agaaagccta tggacggctg
     1261 cgaaacggtt gaccgctgcc acagccgcat cacatgccag cacccgcaat cttatatgga
     1321 cccttggtgg ctcccgcgcc gctcaaacct ctcgtgcccg gccaaccatc atcatctccg
     1381 cgaaattttc cggcgtgatg aagccaacca gccgattgtc cggatcagcc acggcgacgg
     1441 cccgcgcacg cttctgctga agcatctgca caacgttgtc gagcgtcgct gtgccggcga
     1501 ctatgggcac gtccttatgc atgaactcga ccactggcgt gctgccgcca cgatcgcgca
     1561 acacctcgat gagagcgtcg cgtgtgacga ggccctgcag acgcgagaat tgatccagca
     1621 ccggaaactc ctgctgcgtc gtacgcagaa gcagggccac ggcatcgtcg gccgtcgagg
     1681 tcggggttag ggcctcgaac ttggtgatca tcgcatcgcg cgcgagatag ccgcgggcca
     1741 acgagcgcat ctccacgtac tggctttccg ccgtggcggc caggtagacg aacacagcga
     1801 tcaggatcag aagcgggttt ccgagaaggc cgatgaagcc aaggaaaatc gccaacgact
     1861 gccccaccac ggccgccgtg cgggtcgcgc gcacgaatcc catgcgcatg gcaagcagcg
     1921 ccctgagcac acggccgccg tccatcggga aggccgggat caagttgaag accacgagcg
     1981 cgacgttggc gatggcgatc cgcgacagca tgtcggacgc gggattttcc atcaggttga
     2041 gcgccgccgg atcgagacga gcccccgcaa acacgaccag aatgaaagca atgacgacgt
     2101 tcacggccgg gcccgccagc gccacgaaga cctcctggct tggcttctcc ggcatgcgct
     2161 ccaggctggc gaggccgccg atcggcaata gcgtgatg
//
LOCUS       DASUMG010000405         2767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_374101, whole genome shotgun sequence.
ACCESSION   DASUMG010000405 DASUMG010000000
VERSION     DASUMG010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374101"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFX46_06690"
     CDS             <1..403
                     /locus_tag="VFX46_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5600302.1"
                     /translation="KCAAGMMALDALSPDGERGVIINTASVAAQDGQIGQVAYAASKG
                     GVAAMTLPVARDLSKDGIRVCSILPGLFETPMFAGLPEEARTSLAASVPFPSRLGNPS
                     EYAMLAVHICENVMLNGACIRLDGAIRLAPR"
     gene            complement(447..746)
                     /locus_tag="VFX46_06695"
     CDS             complement(447..746)
                     /locus_tag="VFX46_06695"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX5600303.1"
                     /translation="MPEKLSQEARAAALARLPHWQVVEGRDAISRQFKFADFNAAFAF
                     MTRVALVAEKMDHHPEWTNVWNRVDITLTSHDAGGLTEPDVKLAEAIEQLAAKHT"
     gene            complement(797..1366)
                     /gene="thpR"
                     /locus_tag="VFX46_06700"
     CDS             complement(797..1366)
                     /gene="thpR"
                     /locus_tag="VFX46_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX5600304.1"
                     /translation="MPRLFTGIELPDDIRAELARLRRPLPGARWIEPEDYHITLRFAG
                     DIDNEEATEFAHALSEIDVRTFELRFSGMGAFGGNEPRSLWARIEPSPDLDALARANE
                     RAARRSGLQPETRKFKPHVTIARLRNTSVDVVARYLQRFGGFRTRPFTVSRFVLFSSK
                     PQVGGGPYVIESTYTLQGGQYAEFEDEYW"
     gene            complement(1409..2722)
                     /gene="hslU"
                     /locus_tag="VFX46_06705"
     CDS             complement(1409..2722)
                     /gene="hslU"
                     /locus_tag="VFX46_06705"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEX5600305.1"
                     /translation="MTTFSPREIVSELDRYIIGQHEAKRAVAIALRNRWRRQQLDESL
                     REEVLPKNILMIGPTGVGKTEISRRLAKLANAPFIKVEATKFTEVGYVGRDVESMIRD
                     LVEVGVALVRETKRREVRARAEKMAEERVLDALVGENASAATREAFRKRLRANELNDR
                     EIEIRVTDTGGGIPMFELPGVPGGSIGAINLSDLLGKAFGGRQKTRRVTVKDSYDILI
                     AEESDKLLDQEQIIQEAIRAVENNGIVFIDEIDKICSRSEGGRFGADVSREGVQRDLL
                     PLIEGTTVSTKYGPVKTDHILFIASGAFHLAKPSDLLPELQGRLPIRVELKPLTREDF
                     RRILTEPKASLIKQYVALMATEGLTLEFTEDAIDAIADTAAYVNSTVENIGARRLQTV
                     MERVLDEISFDASDRSGQTVVIDAEYVKERVGELAKNTDLSKFIL"
BASE COUNT          508 a          857 c          828 g          574 t
ORIGIN      
        1 gaagtgcgcc gcgggaatga tggcgctcga tgctctttcg cccgatggag aacgcggcgt
       61 tatcatcaat acggcgtcgg tagcagcaca ggatggccag atcgggcagg tcgcctacgc
      121 cgcctcaaag ggtggcgtag ctgcaatgac ccttcccgtt gcccgcgatc tctccaagga
      181 tgggattcgc gtttgcagca tcctcccggg gctgtttgag acaccgatgt tcgctggtct
      241 tccggaggaa gcgaggacgt cgctggcagc cagcgtgccg tttccctctc gcctgggcaa
      301 cccttccgag tacgccatgc tggccgtgca catatgcgag aatgtcatgc tgaacggggc
      361 ctgcatccgc ctggatggtg ccatccggct cgcacctcgt tgagttgtgc ggcggcgctc
      421 gcgcgcgaat aacccgagat gttggttcac gtgtgcttgg ccgcaagctg ctcgatggct
      481 tcggccaact tcacatccgg ttctgttaat ccgccagcat cgtggctggt gagtgtgatg
      541 tcgactctat tccaaacgtt cgtccactcg ggatggtgat ccatcttctc cgcgaccagc
      601 gccacacgcg tcatgaaggc gaaggcggcg ttgaagtcag caaatttgaa ctgccgcgag
      661 atggcgtcgc ggccttcgac aacttgccag tgaggtaagc gagcaagagc ggcagcgcgc
      721 gcctcttggg atagcttctc gggcatgatg gctcctccaa gtccgccaga tcgcaatgtg
      781 ggcagcgagg cgctgctcac cagtactcat cttcgaactc cgcgtattga ccgccctgaa
      841 gggtgtacgt gctctcgatg acgtaggggc cgcccccgac ctgcggtttg gacgagaaca
      901 gaacgaatcg gcttacggtg aacggccggg tacgaaaacc accgaacctc tgtaggtacc
      961 gagcgacgac atcgacgctc gtattcctca ggcgggcgat ggtgacgtgc ggcttgaact
     1021 tgcgtgtttc cggctgaagt cctgaccttc gggcggcgcg ctcgttggcc cgagccagcg
     1081 catccagatc gggactcggc tcgatcctgg cccaaagtga ccgcggttca ttgccgccga
     1141 atgcgcccat cccggagaac cgcaactcga aagtgcggac atcgatctca ctcagcgcgt
     1201 gggcaaactc agtggcctcc tcgttgtcaa tgtcccccgc aaagcgcagg gtgatgtgat
     1261 aatcctccgg ctcgatccag cgcgcgccgg gtagcggccg tctgagacgc gcgagctccg
     1321 cgcgaatatc gtccggaagc tcgattccgg tgaacaggcg gggcatggcc gaactctcca
     1381 ctcgggcctc gcatccggtg cgcgaggtct atagaataaa cttgctcaag tcggtgttct
     1441 tcgcgagctc tccgacccgc tctttgacgt attccgcgtc gataacgaca gtctgtccag
     1501 aacggtcgga agcatcgaac gagatttcat caagcactct ttccatcacc gtctgcagac
     1561 gccgtgcacc gatattctcg actgtcgaat tcacgtaggc tgcggtgtcc gcaatcgcgt
     1621 ctatagcgtc ttcggtgaac tcgagggtga ggccttcggt tgccatcaga gcaacgtatt
     1681 gcttgatgag gctcgctttc ggctccgtca ggatgcggcg gaagtcctcg cgcgtcagcg
     1741 gcttcagctc gacgcggatt ggcaagcggc cttggagctc cggtagcaga tcggaaggct
     1801 tcgccagatg gaaggcaccc gaggcgatga acaaaatgtg gtcggtcttg accggcccat
     1861 atttggttga gaccgtcgta ccttcgatca acggcaagag atcgcgctgg acgccttcgc
     1921 gggaaacatc tgcaccaaac cggcccccct cgctacggga gcagatcttg tcgatctcgt
     1981 cgatgaacac aatgccgttg ttctcgacgg ctcgaatcgc ctcctgaatg atctgctctt
     2041 ggtccaagag cttatcgctc tcctcggcga tcaggatgtc atagctgtct ttgaccgtca
     2101 cccgccgggt cttctgccgg ccgccgaagg ctttaccaag tagatcactc aggttgattg
     2161 ccccgatgga gccgccgggt acgcccggca actcgaacat cggaatgccg cccccggtat
     2221 ccgttaccct tatctcgatt tccctatcgt tgagctcatt ggctctcagc cgcttgcgga
     2281 acgcctcgcg ggtagcggca gaagcatttt ctccgacgag cgcatcgagg acacgctctt
     2341 cggccatctt ctcggcccgg gcgcggacct cccgtcgctt cgtctcccgc acgagggcca
     2401 caccgacctc gacgaggtcg cgaatcatcg actcgacgtc gcgcccgaca tagccgacct
     2461 cggtgaactt ggtggcttcc accttgatga acggggcgtt ggccagcttg gcaagccgcc
     2521 gcgaaatctc ggtcttgccg acaccggtcg gcccaatcat caagatgttc ttcgggagaa
     2581 cctcctctct cagagactca tcgagctgct ggcggcgcca gcgattgcgt aaggcgatcg
     2641 cgacggcccg cttggcctcg tgttggccga tgatgtaacg gtccagctcg gagacgatct
     2701 cgcgcggtga gaaggtggtc atcaggatct acccagctct tggacacgag cgtcaagcgt
     2761 caggccg
//
LOCUS       DASUMG010000406         2968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_775178, whole genome shotgun sequence.
ACCESSION   DASUMG010000406 DASUMG010000000
VERSION     DASUMG010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775178"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VFX46_06710"
     CDS             complement(<1..172)
                     /locus_tag="VFX46_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX5600306.1"
                     /translation="MTEKITFELDGKLVEANEGETIWQVAQRYGTTIPHLCWHPAPGY
                     RADGNCRACMVEI"
     gene            304..756
                     /locus_tag="VFX46_06715"
     CDS             304..756
                     /locus_tag="VFX46_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX5600307.1"
                     /translation="MTLPSTIAHAVQQTQEWLKELRDNGGLASEAEAYAVLRAVLHQL
                     RDRLTLAEAVDLGQQLPLIIRGIYYEGWRPSHAPEKIRTKQKFLDQVTMRLLPHQIPP
                     EPAVRDVFALLAHHLDPGEIADVIDQLPAEIKELWPETARTFRARGGG"
     gene            complement(818..1108)
                     /locus_tag="VFX46_06720"
     CDS             complement(818..1108)
                     /locus_tag="VFX46_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5600308.1"
                     /translation="MTAPRALPTVQVDNERVRVTEWRFAPGAETGFHVHEMDYVVVPT
                     GNGRLKLVSPEGEETYADLKQGVSYFRKAGVHHNVINASEGEFAFIEIELKG"
     gene            complement(1105..2811)
                     /locus_tag="VFX46_06725"
     CDS             complement(1105..2811)
                     /locus_tag="VFX46_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX5600309.1"
                     /translation="MSNSGSYGGGGRAHHPGSGRRKAPMFPKGRVLRQEEVEAVRQLI
                     GPGPYERALLIEYLHKIQDAEHCLPAGYLHGLAELMRIPMAEVYEVATFYAHFDVVND
                     GEPRPPAVTVRVCDSLSCMLAGAEELLKALQQEQLPNVRVVRAPCMGSCDMAPAVEVG
                     HRHVDRATLERVLAVVESGNTHPNVPAYQDLEAYRAEGGYKVLEACLSGERIVEDVIA
                     TLSDGGLRGLGGAGFPTGRKWSLVRAEPGPRFMAVNGDEGEPGTFKDRYYLERRPHQF
                     LEGMLIGAWAVEASEVFIYMRDEYPAVLEILRREVPKLEAAGLSRHTKVHIRRGAGAY
                     ICGEESAMIESIEGKRGLPRHRPPYVAQVGIFGRPTLVNNVETLYWVPQILAKGAEWF
                     ASQGKNGAKGFRSYSVSGRVKNPGVVLAPAGSTVRDLIELCGGMADGHTFKGYLPGGA
                     SGGILPASMADIPLDFGQLEKHGCFVGSHAVVILSDKDDMKAVALNLMKFFRDESCGQ
                     CTPCRNGTEKAVKLMSEPHWDKELLEDLAQVMADASICGLGQAAPNPLRSVLKYFPED
                     VR"
BASE COUNT          542 a         1015 c          862 g          549 t
ORIGIN      
        1 cgatctccac catgcacgcc cggcagttgc cgtcagcgcg gtagcccggc gccgggtgcc
       61 agcacaggtg cgggatggtc gtgccgtacc gctgcgcgac ttgccagatg gtctcgccct
      121 cattggcctc gacgagcttg ccgtcgagct cgaacgtgat cttctcggtc atatccaacc
      181 cctggcccaa cctgtgccgc tgttcgtgcg ttttgcactc gcgccgattt gatctggcgc
      241 attcacgagg cgcctaatgc gatcgaggat cttgcccggg acattcccca gggagtgatg
      301 acgatgacat tgccatctac catagctcat gccgtgcagc agacgcagga atggctgaaa
      361 gagctgcgcg acaatggcgg attggcgagc gaggccgaag cttacgcggt acttcgcgct
      421 gtccttcacc agctgcgcga ccgcttgacc ctcgccgaag ccgtcgatct cggccagcag
      481 cttccgctca tcatacgcgg aatctactac gagggctggc ggccgtcgca tgcaccagaa
      541 aagatccgca ccaagcagaa gttccttgac caggtcacga tgcgcctgct gccgcaccaa
      601 attccgcccg agccggcggt ccgcgacgtg ttcgccctgt tggctcacca cctcgatccc
      661 ggcgagatcg cggacgtgat cgatcagctt cccgccgaga tcaaggagct gtggcccgaa
      721 acggcgcgga ccttccgtgc gcgcggcggt ggctgatatg accgaggagg agatcactga
      781 tctaacgcgc tcctggaaac gcctgacctc atcgacctca gcccttgagc tcgatttcga
      841 tgaacgcgaa ctcgccctcg ctcgcgttga tgacgttgtg gtgcacgccg gcttttcgaa
      901 aataggagac accctgtttc agatcggcgt atgtctcctc cccttcaggc gaaaccagct
      961 tcagccgtcc attgcccgtg ggcaccacga cgtaatccat ctcgtgcacg tgaaagcctg
     1021 tctccgcccc aggggcgaag cgccactcgg ttacgcgcac ccgctcattg tcgacctgca
     1081 ccgtcggcag ggcgcgggga gctgtcatcg aacatcctcc gggaaatatt tgagcactga
     1141 tcgcaaggga ttgggggcgg cctgacccag gccgcagatg gaggcgtctg ccatcacctg
     1201 cgccaggtct tcaagcagct ccttgtccca gtgcggctcg gacatgagct tcaccgcctt
     1261 ctccgtgccg ttccggcacg gggtgcattg gccgcagctc tcgtcccgga agaacttcat
     1321 gaggttgagg gccaccgcct tcatgtcgtc cttgtcggac aggatgacga cggcgtgact
     1381 ccccacgaaa catccgtgtt tctcgagctg accgaaatcg agcggaatgt cagccatcga
     1441 agccggcaaa atgcccccag aggctccgcc cggcagatag cccttgaacg tgtgcccgtc
     1501 ggccatccca ccgcaaagct caataagatc ccgaacagtc gagcccgcag gtgcaagcac
     1561 cacgccggga ttctttacac gtccggaaac ggagtatgag cggaagcctt tcgcaccatt
     1621 cttgccctgc gacgcgaacc actccgcgcc tttggccaga atttgcggca cccagtaaag
     1681 cgtctcgaca ttgttgacga gcgtcggccg cccgaatatg cccacttgcg cgacgtatgg
     1741 cggccgatgc cgcggcagac cgcgctttcc ttcgatcgac tcgatcatcg cgctttcttc
     1801 accgcagatg taggcgccag cgccgcggcg aatatggact ttcgtgtggc gtgataggcc
     1861 cgcggcttcg agcttcggca cctcgcgtcg caaaatttcg agcacggcgg gatattcatc
     1921 ccgcatgtag atgaaaacct cggatgcctc gacggcccag gcgccgatca gcatgccctc
     1981 gaggaactgg tgtgggcggc gttccaggta ataccggtcc ttgaacgtgc ccggctcgcc
     2041 ctcgtcgccg ttgaccgcca tgaaccgcgg gcctggctct gcgcgcacga ggctccactt
     2101 gcggcccgtt ggaaatcccg caccgccgag cccgcgcagg ccgccatccg aaagcgtcgc
     2161 gatcacatcc tcaacgatgc gctcgcctga aagacaggct tcgagcacct tgtaaccgcc
     2221 ctcggcgcga taggcctcga ggtcttgata ggctggaaca ttcggatgcg tgtttccgga
     2281 ttccactaca gcaagcacac gctccagcgt ggcgcgatcg acatgccgat gccccacttc
     2341 gaccgcgggc gccatatcgc aagagcccat gcacggcgcg cggacgacgc gcacattcgg
     2401 cagctgctcc tgctggagcg ccttcagaag ctcctctgcg cccgcgagca tgcaggagag
     2461 gctatcgcag acgcgcaccg tgacggccgg cggccgcggc tctccgtcgt tcaccacgtc
     2521 gaagtgcgcg tagaacgtgg ccacctcgta aacctccgcc atcggaatgc gcatcagctc
     2581 agcgaggcca tgcagatagc cagccggcaa acagtgctcc gcatcctgaa ttttatgcag
     2641 gtactcgatg agcagtgcgc gctcatatgg gcccggaccg atcaactgcc gtacggcctc
     2701 gacctcctcc tggcgcagca cgcggccttt cgggaacatc ggcgccttgc gccggcctga
     2761 accggggtga tgggcccgac ctccgccgcc gtatgatccc gagttgctca tatcgttcat
     2821 gtcagctcac caatgcctgc cgcatcgtca tgcccacgag atccggccgc ggcgcaatga
     2881 tcacgcccgc ctctcgcagc gcattgatct tggcctccgc gctaccggcc cggccaactg
     2941 ccagcgcgcc ggcgtggccc attcttcg
//
LOCUS       DASUMG010000407         2837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_614614, whole genome shotgun sequence.
ACCESSION   DASUMG010000407 DASUMG010000000
VERSION     DASUMG010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614614"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VFX46_06730"
     CDS             <1..1233
                     /locus_tag="VFX46_06730"
                     /inference="COORDINATES: protein motif:HMM:NF019174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HWE histidine kinase domain-containing protein"
                     /protein_id="HEX5600310.1"
                     /translation="SFEPELDVIRKVIEQTFLPDEWRAAVIDGNGRMVARSSQHEQFY
                     GRLTSEEWRAGLQGPYGFIEAVDFEGREVLTSYRRSGASDWYILIWAPKSALNDPLYR
                     SLFLALGLAAMMLLPSIAAGLLAGRAVARPIQQLLDTARAIEAGQPVEFQPTHQREAN
                     IVGHALAEAARSIAAREQALRKSELHTRFVMRELAHRSKNLLAVVQAIARQTGRSARD
                     IDDFTAQFGERLAGLGRSQDLLVQKNWQGVSLSDLVSAQLKPFVEADNNRVDANGPPV
                     LLDADAAQNIGMALHELATNASKHGALSVPTGRVDIAWGWLPSEIGERGRRLQLQWSE
                     SGGPPVTPPKRKGFGHSVIERLTASSLHGTAKLDWRPEGLLWTLEVPEGSITKSEQNE
                     VEASSPTQFETANSVPEH"
     gene            complement(1274..2092)
                     /locus_tag="VFX46_06735"
     CDS             complement(1274..2092)
                     /locus_tag="VFX46_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX5600311.1"
                     /translation="MAFPPERIVCLTEETVETLYLLGEEARIVGVSGYAVRPPRVRKE
                     KPRVSAFISADIPKILALEPDLVLAFSDLQADIVAGLIREGVAVHAFNQRTVAGILDM
                     IRVLAAMVGAQAKGEELAGKLQKRLESVAAAAKSLPRRPKVYFEEWNAPMISGIGWVS
                     ELIQLAGGIDVFASNAARQSAKERIVTEEDVIAAGPDIIFGSWCGKKFRPEQVRARKG
                     FDSIPAVRNGNIFEIKSTIILQPGPAALTDGLDAIAEHIRRWAYDHPQKADHGQ"
     gene            2250..>2837
                     /locus_tag="VFX46_06740"
     CDS             2250..>2837
                     /locus_tag="VFX46_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX5600312.1"
                     /translation="MANPRIELHKGDLPAGLDLGPSVAVDTEALGLKPQRDRLCLVQL
                     SAGDNTAHLIQFTRGDYSAPRLKALLTDPAVLKIFHFARFDIAILKAYLGVTTAPLYC
                     TKIASKLARTYTDRHSLKDLVSEFVGIELSKQQQCSDWGAAELTQQQLQYAASDVLYL
                     HAVKARLDAMLEREGRQHLAKACFDFLSTRAELDLL"
BASE COUNT          583 a          861 c          839 g          554 t
ORIGIN      
        1 tcctttgagc ccgagctgga tgtcatccga aaggtaatcg aacagacctt cttgccagac
       61 gaatggcgcg cggctgtcat agatgggaac gggcgtatgg ttgcccgctc atcgcagcat
      121 gagcaatttt acggcaggtt aacgtccgag gagtggcgcg cggggcttca agggccgtac
      181 ggctttatag aagcggtcga cttcgagggt cgcgaggtgc tcacgagcta ccggcgaagt
      241 ggcgcgagcg attggtatat tctgatctgg gcacctaagt cagcgctcaa cgatccgcta
      301 tatcgttcgc ttttcttggc actggggctc gctgcaatga tgcttcttcc atcgattgct
      361 gcgggtctcc ttgccggccg cgccgtcgca agaccaattc agcagctcct ggatactgcc
      421 cgggcaatcg aagcggggca gcccgtcgag ttccagccaa ctcatcaacg cgaagcgaac
      481 atcgtcggcc acgcacttgc cgaggccgcg cgcagcatcg ccgcacgcga gcaggcgctg
      541 cgtaagagcg agttgcacac ccggttcgtc atgcgcgagt tggctcatcg ttccaagaac
      601 ttgttggcag tggtccaggc gatcgcgcgg caaaccggcc ggtctgcgcg ggatatcgac
      661 gacttcacgg cgcaattcgg agagaggttg gcggggctcg gccgctcgca ggatcttctg
      721 gtgcagaaaa actggcaggg cgtgtcgttg agcgatcttg tatccgcgca gctcaagccg
      781 ttcgtcgagg ccgacaacaa cagagttgat gccaacggcc cgccagtgct cctcgatgcg
      841 gatgccgcgc agaacatcgg catggcttta catgagctgg ctaccaatgc ctcgaagcac
      901 ggtgcacttt ccgttccgac cggtcgcgtc gatatcgctt ggggctggct gccgagcgaa
      961 attggcgagc gagggcgaag gctgcaactg caatggagcg agtccggcgg gcctcccgtt
     1021 acgcctccga aacgcaaagg gttcggacac tccgtcatcg agcgccttac cgcatccagc
     1081 ctgcacggca cggccaagct cgactggaga ccagaaggac tgctctggac cctcgaggtg
     1141 cccgagggct cgataacaaa gagcgagcag aacgaggtgg aggcttcttc acctacccaa
     1201 ttcgaaaccg cgaattcagt tcctgagcac taattggcgt acacgcggtg caatttgcga
     1261 gggggccgtc acgctactgt ccgtggtcag ccttttgagg atggtcgtac gcccaacgtc
     1321 gaatgtgctc agcaatagcg tctaacccat ccgtcagagc ggcgggtcca ggctgcagga
     1381 taatcgtcga tttgatttcg aagatgttgc cgttacgaac agcgggaatg gagtcgaatc
     1441 ccttgcgggc ccgcacctgc tcagggcgaa atttttttcc gcaccacgaa ccgaaaatga
     1501 tgtccgggcc agcggcgata acatcttctt ccgtgacaat ccgctctttc gccgactggc
     1561 gcgcagcatt cgaagcgaag acatcgatac caccagccaa ttggatgagc tccgaaaccc
     1621 acccgatccc ggagatcatg ggagcattcc actcttcgaa gtagaccttg gggcgacggg
     1681 gcagcgactt cgcggcggca gccactgact cgaggcgctt ctgcaatttt ccggctagct
     1741 cctcaccctt cgcctgagca cccaccatag ctgcgagcac gcgaatcata tccaggatgc
     1801 cggcaaccgt gcgctggttg aaagcgtgca cggcgacccc ttcccgaatg aggccagcga
     1861 cgatgtctgc ctggagatca gagaaggcaa gcaccaggtc cggctcgagg gcgaggatct
     1921 tcggaatgtc ggcgctgatg aacgcagaga cacgcggctt ttctttccga acgcgaggtg
     1981 gtcgcaccgc gtatccggag actccgacga tccgcgcttc ctctccgagc aaatagagag
     2041 tctcaactgt ctcctcggtc aggcagacga tccgctcagg tgggaaggcc atgggcatgc
     2101 ctccatttgg gtttcgcgca tcggccatta ctctgcgtgg agacccagta gcggacccgc
     2161 ttgttaagga tcaggccgga tgctaggagt gtcaaatcga gcttcagcat gaggaaagtt
     2221 cgccgaagct caatgactta ggggtcgaga tggccaatcc gcgcatcgag ttgcacaagg
     2281 gtgatctgcc ggccgggctc gatctcggcc catcggttgc tgtcgacacg gaagccctag
     2341 gcctcaagcc tcaacgagac cggctctgcc tggtccagct ttcagcgggc gacaacacag
     2401 cacatctcat ccagttcacg cgtggcgatt attccgcccc gcggctcaag gcgctgctga
     2461 ccgaccctgc cgtactcaag atctttcact ttgcccggtt cgacatcgct attctcaagg
     2521 cctacctggg cgtcaccacc gcaccgctct actgcacgaa aatcgcctca aagctggctc
     2581 gcacgtacac agatcgccac agcctcaagg accttgtcag tgagttcgtt ggcatagagc
     2641 tctcaaaaca acagcaatgc tccgactggg gtgctgcaga gctgacgcag cagcagcttc
     2701 agtatgccgc ctccgacgtg ctctatcttc acgccgtaaa ggcgcgactt gacgccatgc
     2761 tcgagcggga aggacggcaa cacttggcca aggcctgctt cgattttctg tctacgcgcg
     2821 ccgagctcga cctgctc
//
LOCUS       DASUMG010000408         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_775234, whole genome shotgun sequence.
ACCESSION   DASUMG010000408 DASUMG010000000
VERSION     DASUMG010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775234"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1229)
                     /locus_tag="VFX46_06745"
     CDS             complement(207..1229)
                     /locus_tag="VFX46_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5600313.1"
                     /translation="MMMKRKMRGRGVAASWYGIARTATIDRAGAWAELDDGGTAKIVT
                     GVTEIGEGILTVLAQIAAEELGIRPEDVTIGDNDTARAPEAAHAGATRQTYMIGNSVA
                     LACREARAKLVRELAAHWDVPEDSIVAFNGEIWSESSNLKMTMAEAVDICKKRGVVPV
                     GSGSFTSYGTGLDPVDGSGSPWQAYVFGCQVAEVEVDTMTGEVQVLGIWAAHDVGRAI
                     NPRGIEGQIEGGIAQGLGQALMEEYKTVNGYTTTPSLAKYILPTALDVPKINSIIIED
                     PDPRSPLGAKGIGEPALIPTAPAIINAIYDAVGVRITSLPATPEKILAALEEKARRES
                     QRQAAE"
     gene            complement(1226..>2600)
                     /locus_tag="VFX46_06750"
     CDS             complement(1226..>2600)
                     /locus_tag="VFX46_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5600314.1"
                     /translation="GKSYIGSNVRRIDAPSKVSGRLRYAGDMVMPGMLHMQVLRSPHP
                     HALIKSIDTSAAEALEGVACVITSADVPGKDGFGVFIHDQPVMARGKVRYVGEAVAAV
                     AAEDIVTARKALSLIKVEYEPLPAIFDPEEAMRPGAPVLHDYAKDNIVKHIPIRKGDI
                     EKGFAEADIIVEEKYETQQVEHAYLEPEAGLAYVDHDGVVTVISPSQNITHHRHMLAE
                     ILAKPINKVRFIMSPVGGGFGGKEDMIYQGMLAIAALKTHRPVRLVFTREESIISTAK
                     RHPARITYRMGLKRNGRITAVSFRMISDGGAYAMSTEGVMRKAAILACGPYDIPNLQV
                     DTYGVYTNNTPSGAFRTFGAMQVQFATEAHLDLCAEKLGLDPFEIRRINMMRDGALTH
                     TKQKLGSVSLGRVLDAAEKASRWEKGPPNMRGPYRSDLGGEDTRPPCTLGARFRKVET
                     PARQEVA"
BASE COUNT          450 a          859 c          738 g          553 t
ORIGIN      
        1 gtaatccttc atccagccta cctctacggc ggtccagaag tcgtccgcgc agatcttctc
       61 cagcgtctcc aagattgggc cggtgccgga ctccacgcct gggaatgcct tgaaatggac
      121 gacccctaca cgcatgaacc ggtacatact agcgtccatg ctctcctcca gctcctcgcc
      181 cttcacaact ctattacttc attacttcac tctgcagctt gcctttggct ctcccgcctc
      241 gccttctcct caagtgccgc tagaattttc tcgggcgtag caggcagcga cgtgatcctg
      301 accccaacag cgtcgtaaat ggcgttgatg attgctggtg ccgtcggtat cagggccggc
      361 tcgccgatac ccttcgcccc cagcgggctg cgcggatccg gatcctcgat gatgatcgag
      421 ttgatcttcg gcacatcgag cgcggtcggc agaatgtact tcgccaagct tggggtcgtc
      481 gtatagccgt ttacggtctt gtattcttcc ataagagctt ggcccagacc ctgcgcgatg
      541 ccaccttcga tctgcccctc gatgccccgc ggattgatgg cgcggcccac gtcgtgcgcc
      601 gcccagatgc cgagcacctg aacctctccc gtcatcgtgt cgacctccac ctcggccacc
      661 tggcagccga agacgtaggc ttgccagggg ctgccagagc cgtcgactgg atctagacct
      721 gtgccgtacg acgtgaacga tcctgagccg accgggacca cacccctctt tttgcaaata
      781 tcgacggcct ccgccatcgt cattttcagg ttggagcttt cggaccagat ttcgccgttg
      841 aacgcgacga tgctgtcctc aggcacatcc cagtgagcgg ccagctcacg aacgagcttg
      901 gcgcgggcct cacggcaggc cagcgcgacc gagttgccga tcatatacgt ttgacgcgtg
      961 gctccggcgt gcgccgcctc gggagcccgc gctgtatcgt tatcaccgat cgtcacgtcc
     1021 tcgggccgaa tgccgagctc ctcggcagcg atctgcgcca atacggtgag aatgccttcg
     1081 ccaatttcgg ttacgcctgt gacgatcttg gcggtgccgc cgtcatccag ctccgcccag
     1141 gcccctgccc tgtcgatggt cgccgtgcgc gcgatgccat accagctcgc tgcaacgcca
     1201 cggccgcgca tcttacgctt catcatcatg cgacctcctg ccgggctggt gtttcgactt
     1261 tacggaagcg agcgccgagt gtgcacggcg gccgtgtatc ttctccgccg agatcggagc
     1321 ggtacggccc gcgcatgttc ggcggcccct tttcccaacg cgaggccttc tcagcagcat
     1381 cgagaacgcg gcccagcgaa actgagccga gcttctgctt cgtgtgtgta agggcgccat
     1441 cgcgcatcat attgatgcgc cggatctcga atggatcgag cccgagcttt tccgcacaca
     1501 ggtccaaatg cgcttccgtc gcgaactgga cctgcatggc gccgaacgta cggaacgcac
     1561 ccgacggcgt gttgttggtg taaacaccat acgtatcgac ctgaaggttc ggaatgtcgt
     1621 acgggccaca ggccaggatc gccgccttcc gcatcacacc ttcagtggac atcgcatatg
     1681 ctccgccgtc tgagatcatc cggaatgaga cggcggtgat gcggccattc cgcttcaggc
     1741 ccatccgata agtgatgcgt gccgggtggc gcttggctgt ggagatgatc gactcttcac
     1801 gcgtaaagac gagccgcact ggccggtgag ttttcagcgc tgctatggcc agcatgccct
     1861 ggtagatcat gtcctccttg ccgccgaagc cgccgcccac cgggctcatg atgaagcgca
     1921 ccttgttgat cggcttggca agaatctccg ccagcatatg gcggtggtgc gttatgttct
     1981 ggctcggcga gatcaccgtc acgacaccgt cgtggtcaac gtaagccaag ccggcctcgg
     2041 gctcgaggta cgcgtgctcg acctgctgtg tctcgtattt ctcctcgacg atgatatcgg
     2101 cctcggcgaa ccctttctcg atgtcacctt tccggatcgg gatgtgcttg acgatgttgt
     2161 ccttggcgta gtcgtgcagc accggcgcgc caggacgcat cgcttcttcc ggatcgaaga
     2221 ttgccggcag cggctcgtac tcgaccttga tgagcgacaa agccttgcgc gccgtgacga
     2281 tatcctcggc cgcgaccgcg gccacggctt caccgacata acgcaccttg ccgcgcgcca
     2341 tgaccggctg gtcatggatg aaaacgccga atccgtcctt gccgggcacg tccgcggacg
     2401 tgatcacgca ggctacgccc tcaagcgctt ctgccgcgct tgtatcgatg gatttgatca
     2461 gcgcgtgcgg atgcggacta cgcagcacct gcatatgcag catgccgggc atgaccatat
     2521 ctccggcata tcgcaggcgg cccgaaacct tgctcggcgc gtcgatccgg cgaacattgc
     2581 tgccgatata tgatttgccc
//
LOCUS       DASUMG010000409         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_53014, whole genome shotgun sequence.
ACCESSION   DASUMG010000409 DASUMG010000000
VERSION     DASUMG010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53014"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFX46_06755"
     CDS             <1..874
                     /locus_tag="VFX46_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX5600315.1"
                     /translation="RSLGGWEKLMETLRKRLEEQQGRHQGGSKWIGTAGTSPYGAYGY
                     NPEGVRIGQDENRNFRAVKVWDKRDFRDLDDEVEIGTRNIKLALRRLRKFARTGAPEE
                     LDLDGTIKGTAHKGYLDILMRPERHNAVKVLLFFDIGGSMDWHIKATEELFSAARSEF
                     KHLQYFYFHNCLYERVWKENRRRFQETTPTWDVLHTYPHDYKVIFVGDASMSPYEIAA
                     PGGSVEHFNDEPGAVWLERMTRTYQACVWLNPVPEKEWNFTQSVGMVRQLMGGRMFPL
                     TLEGLDRAMRELVR"
     gene            complement(1379..1624)
                     /locus_tag="VFX46_06760"
     CDS             complement(1379..1624)
                     /locus_tag="VFX46_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600316.1"
                     /translation="MGELMANGDSRGERLQIMLSGSELTVLDDFRFRKRMPSRAAAIR
                     ELLKRGLAAEGFEAAAFGSKSEEFGVTGKTPIGRDKK"
     gene            1798..1986
                     /locus_tag="VFX46_06765"
     CDS             1798..1986
                     /locus_tag="VFX46_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600317.1"
                     /translation="MKHIVPQEPQSFAPVLCGRCGNVMRLVGSEPHPVEAKTDLLTYA
                     CTGCDEFFVVPIEMHARR"
     gene            complement(2060..3484)
                     /locus_tag="VFX46_06770"
     CDS             complement(2060..3484)
                     /locus_tag="VFX46_06770"
                     /inference="COORDINATES: protein motif:HMM:NF039892.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600318.1"
                     /translation="MNKFGGFFARRAGLYDKPADPTPTPLTDNPLELDEELFTALGAQ
                     LGGENESLRNLLLDANAKIGELDGIKNAVGKLVDPVSKALRAFEAEKSEKISLQTVLN
                     NTRTAYGKLRNEVAELEKKANLSEKECQALRQELTTTQNLLRTLEATKAEIAIDIAAR
                     RAQIVDLEARLAQETGEGKALREENRRLDDRFTAADKRVMALESELNGARQRLLMLED
                     EKRAQQISLDKMSAESARLSRKFAETEASLTATQGRLRHVEGNVAELNSERSRLVGAL
                     DEANERHEHELTTQRMRFDALQARASASEKLLGEAREHLLSRADEIRTYDRRAGELAL
                     ERDALQARVADLEAERIQRESQFKEVEQARATLVERSTALARAFTAKEAALTRAEDAI
                     AALNEQVTALEGARATEKQAADQRIEELSTSLRREQMQCSVVEGALETARKDFARLMR
                     EATALQRAQAGPDDPGLLRPANAA"
BASE COUNT          655 a         1134 c         1152 g          796 t
ORIGIN      
        1 ccgcagcctg ggcggctggg agaagctcat ggagacgctg cgcaagcgcc tggaagagca
       61 gcaaggccgc catcagggcg gatcgaaatg gatcggcact gccggcacgt cgccctacgg
      121 tgcctatggc tacaatccgg aaggtgtgcg cattggtcag gacgagaacc gtaatttccg
      181 tgcggtcaag gtgtgggaca agcgcgactt cagggatctc gacgacgaag ttgaaatagg
      241 cacacgcaac atcaagctgg cgcttcgccg cttgcgaaag ttcgcacgca cgggcgcacc
      301 ggaagagctt gacctcgatg gcaccatcaa gggcaccgcg cacaagggct acctcgacat
      361 cctgatgcgg cccgagcggc acaacgccgt gaaggtgctc ttgttcttcg acattggcgg
      421 ctcgatggac tggcacataa aggcgaccga ggaattgttc tcggccgcgc gcagcgagtt
      481 caagcacctt cagtatttct atttccacaa ctgcctgtac gaacgcgtgt ggaaggaaaa
      541 ccgccgccgt ttccaggaaa ccacgccgac ctgggacgtg ctccacactt atccgcacga
      601 ctacaaggtt atcttcgtcg gcgatgcgtc gatgtcgccc tacgagattg cagcacccgg
      661 cggctcagtg gagcacttca acgacgagcc gggcgcggtg tggctcgaac gcatgacgcg
      721 cacgtatcag gcttgcgtct ggctcaatcc ggtgcccgag aaggaatgga atttcacgca
      781 gtcggtcggc atggtccgcc agctgatggg cgggcgaatg tttccgctca cgctcgaggg
      841 gctcgaccgt gcgatgcgcg agttggtgcg ctaggcacgc cgctcacgta tcgcgcgcga
      901 ccgtcaacag ccggacgacg ccgtgccgtt cgccgacgaa gtctgatttg tgcctgcggt
      961 gggattgctg ctcgacgaca ggctgccgct cttagagccg cttcccaacc ccgtggtcga
     1021 accggatcca gtgccggcga ctgatccaca gggctgaccc ctaagtccgg ttgttcccga
     1081 catcgacggt gtaggtgtgc cgctcgggtt gcttccggtc gtcgatggtg aaccaggcgt
     1141 tgcagccgga ctcgatcctg tgctgctgcc tgtactcgtc ccggcgccgg cgctgccgcc
     1201 cgttccgccg ccactgccac ctccgcttcc gccgcccgat tgggcgagcg ccgcggttcc
     1261 ggcgatcaac aaagctgaaa atatcgcgag ggctgttcca gttctccgca tcacgtgcac
     1321 tcctgttggc tgcgtcaggg ccaacgtaag gcgcagtgcg gagtttcctt ccgccggttt
     1381 atttcttgtc gcggccgatt ggcgtttttc cggttactcc gaattcctca gacttgcttc
     1441 caaaggccgc agcctcgaac ccttccgcgg caaggccgcg tttcagcagc tcgcggatcg
     1501 ccgcggcgcg gctgggcatg cgctttctga agcgaaaatc gtccaggacg gtcagttcgc
     1561 tgccggacag catgatctgg agccgttcgc cccgactgtc accgttggcc atcaattccc
     1621 ccattacatg acttcaatta gatgactaca tatgagtaac ttactcaaag cgattaggtt
     1681 ccgcaagggg gggccgatta gttgctgtgt ataaaggccg aaaaccgggg cttttatcca
     1741 aaattggttg cgaagaaaca gaattgacgc cccttgggag gggcatagcc tgtttctatg
     1801 aaacacatag tcccccaaga gccccaaagc tttgcacctg ttctgtgcgg gcgttgcggc
     1861 aacgtcatgc ggctggtcgg cagcgagccc catccggttg aagccaagac cgaccttctc
     1921 acctatgcct gcacaggctg cgatgagttt tttgtggttc cgatcgagat gcacgctcgc
     1981 cgctaatcgc aacttttgag caataacaac aaagccgggc aatttgcccg gctttgttgc
     2041 gtttcgtccg gtgatcgaac taggcagcgt tggccgggcg caacaggcca ggatcgtccg
     2101 gcccggcctg cgcgcgctgg agggctgtcg cctcgcgcat gaggcgcgcg aaatccttgc
     2161 gggcagtttc gagcgcacct tcgacgaccg agcattgcat ctgctcgcgc ctcagcgagg
     2221 tgctcagctc ctcgatcctt tggtcggccg cctgcttttc ggtcgcgcgc gctccctcca
     2281 gcgccgttac ctgctcgttg agggcagcga tcgcgtcctc agcacgcgtc agcgccgcct
     2341 ccttcgcggt gaaagcgcgg gcgagcgcgg tgctgcgctc gacgagggtc gcgcgggcct
     2401 gttcgacctc cttgaattgg gattctcgct ggatgcgttc ggcctcaagg tcggcgacgc
     2461 gtgcctgcaa ggcgtcgcgt tcgagcgcaa gctcgcctgc acgccgatca taggtcctga
     2521 tttcgtccgc gcgtgacaac aagtgctcgc gcgcctcgcc gagcagcttc tcgctggcgg
     2581 aggcgcgagc ctggagggcg tcgaagcgca tgcgctgggt tgtgagctcg tgttcgtgcc
     2641 gctcgttggc ctcatccagg gcgccgacca gccgcgagcg ctcgctatta agttcggcga
     2701 cattgccttc cacgtgacgc aggcgtcctt gcgtggccgt gaggctcgcc tcagtctcgg
     2761 caaacttgcg ggagaggcgg gcggactcgg cgctcatctt gtcgagtgaa atctgctggg
     2821 cgcgcttttc gtcctccagc atcaggagac gctgccgcgc gccgttgagt tcggattcca
     2881 gcgccatcac acgcttgtcc gcggcggtaa atcggtcgtc gagcctgcga ttttcctcgc
     2941 gtagcgcctt gccttcgccg gtttcttgcg caagccgcgc ctcgagatcg acgatctgcg
     3001 cgcgccgtgc ggcaatgtcg atggcgatct ctgctttcgt ggcttcgagc gttcgaagca
     3061 ggttctgcgt cgtcgtaagc tcctgacgca gcgcctggca ttccttttcc gagaggttcg
     3121 cctttttctc gagttcggca acctcgttac gcagcttgcc gtaagcagtg cgcgtgttgt
     3181 tgagcaccgt ctgcagactg atcttttcgg acttttcggc ctcgaaggcg cgtagcgcct
     3241 tgcttaccgg atcgaccagc ttgccgaccg cattcttgat gccgtcgagc tcgccgatct
     3301 ttgcgttggc gtcgagcaga aggttgcgga gcgattcatt ctcgccgcca agctgggcgc
     3361 cgagcgccgt gaacagctct tcgtcgagtt cgaggggatt gtcagttaaa ggggttggcg
     3421 tcggatcggc cggtttgtca tagagtccgg ctctgcgggc aaaaaagccc ccgaatttgt
     3481 tcattgggtg atcccctaaa cgccgattcg cccggccgaa gcgacgccga atcacactgt
     3541 taatagatcc taacggaatc taaatttctg tgggtaaatg aaaggttaac gaccaaattc
     3601 gttgatttgt caggtggttg gccaagggca caggtttagc cacaagtctg caaaattgca
     3661 gaatttcggt tgccttaaat cggcgccggg gagccgagcg ttgcgctgcg gtcaggtccg
     3721 tttggcgatg aacaaac
//
LOCUS       DASUMG010000410         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_133876, whole genome shotgun sequence.
ACCESSION   DASUMG010000410 DASUMG010000000
VERSION     DASUMG010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133876"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VFX46_06775"
     CDS             complement(<1..1167)
                     /locus_tag="VFX46_06775"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5600319.1"
                     /translation="MTQAAAFEAGRRFEVERYRADFPILFREVYGKPLVYLDSAASAQ
                     KPLSVIEAMDHALRFEYANVHRGIHLLSNTATQKYEDARETVRRFLNAPSAEEIVFTK
                     NATESINLVAYSFGLENIQEGDEIVLSIMEHHSNIVPWNFLRERKGAVLKWVPISDTG
                     EFLLDEFERLLGPRTKIVAVTHVSNVLGTVVPVKEVCRIAHERGIPVLVDGSQGAVHL
                     PVDVQDIGCDFYAFTGHKVYGPSGIGVLWGRKSLLESLPPFLGGGSMIESVTVDGITY
                     GPPPTRFEAGTPPIVEAIGLAAALNYMMQIGRDEIAAYEHDLVTYAHERLGEFNWLRI
                     FGTARDKSAIVSFSVEGLHPHDVSTIVDRSGIAIRAGHHCAQPLMDRYGVPAMCR"
     gene            complement(1182..2492)
                     /gene="sufD"
                     /locus_tag="VFX46_06780"
     CDS             complement(1182..2492)
                     /gene="sufD"
                     /locus_tag="VFX46_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5600320.1"
                     /translation="MTKTKAEQAFTETFEAVAAKLPGSEAVRELRRKAIGTFAALGLP
                     HRRIEEWKYTDLRSLLKEVFAPAIGKAAAPSEADLNAGLGPLGALDTYRVVVVDGAFN
                     AGLSTAQLPDGLSVKPLSEVLAESPDIVRMKRDQDAIEALNSAFATDGAVVRIADGTE
                     LDKPLLLVFARAGADRKTITTRNVISIGKGSKVTLVEAYVPLAGAADEDQHNAATEVV
                     VGEGAAVTHVKAIVENGRAVHLGSATTEIGANATYRIFQLTAGPGLARNQIFVKFAGE
                     GGKLDCSGSFLATNNEHIDTTLLVDHAVPHCESRELFKGVLDGHGRGVFQGKVIVRPG
                     AQKTDGKQMAQALMLSPDAEFDSKPELEIYADDVVCGHGSTAADIDPEHIFYCRARGI
                     PEPEARRLLIEAFVGEVMEKVEDEPVREALMETARVWLNQRTAH"
     gene            complement(2525..3277)
                     /gene="sufC"
                     /locus_tag="VFX46_06785"
     CDS             complement(2525..3277)
                     /gene="sufC"
                     /locus_tag="VFX46_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5600321.1"
                     /translation="MLEIRNLHVKIADEDTMILKGVNLIVPKGEVHAIMGPNGSGKST
                     LAYVLAGREDYEVTEGEILWNGENLLEMEPDERAVKGVFLAFQYPIEIPGVAGLTFLR
                     TALNALRKRREQPELTTPEFMKLVRERAAKLNIDVEMLKRPVNVGFSGGEKKRSEILQ
                     MALLEPTLCVLDETDSGLDIDALRIVADGVNALRSPDRAMVVITHYQRLLNYIVPDKV
                     HVLADGRIQRTGDKELAVELERSGYAEFTKAA"
     gene            complement(3333..3605)
                     /locus_tag="VFX46_06790"
     CDS             complement(3333..3605)
                     /locus_tag="VFX46_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600322.1"
                     /translation="MSTEIALLEQAWLEAETAADALKLEAAKASAELARMRQSAGANG
                     ADLSALVAMVEQLKARQEEAERAASAAFDRYWAAQGNGKDSGSAYA"
     gene            complement(3723..>4535)
                     /gene="sufB"
                     /locus_tag="VFX46_06795"
     CDS             complement(3723..>4535)
                     /gene="sufB"
                     /locus_tag="VFX46_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX5600323.1"
                     /translation="INERNTGQFERTLIIADKGAYVSYLEGCTAPQRDENQLHAAVVE
                     LVTLDDAEIKYSTVQNWYPGNSEGKGGIYNFVTKRGDCRGHSSKISWTQVETGSAITW
                     KYPSCILRGDGSRGEFYSIAISNGYQQVDSGTKMIHLGKNTSSRIISKGIAAGYSQNT
                     YRGLVSAHRKATNARNFTNCDSLLIGDKCGAHTVPYIEAKNSTAHFEHEATTSKISDN
                     QLFYCMQRGLSEEEAVALIVNGFVRDVIQQLPMEFMAETQKLIGISLEGSVG"
BASE COUNT          751 a         1524 c         1356 g          904 t
ORIGIN      
        1 acggcacata gccggcacgc catatcgatc catcaagggc tgcgcgcagt ggtggccggc
       61 gcggatggcg atgcctgagc gatcaacgat cgtcgacacg tcgtgcgggt gcaggccctc
      121 gacgctgaat gatacgatgg cgctcttgtc ccgggcggtt ccgaaaatcc taagccagtt
      181 gaattcgccc agccgctcgt gggcgtacgt gacgaggtcg tgttcgtagg cggcgatctc
      241 gtcgcgcccg atctgcatca tgtaattcaa cgccgccgcc aggccgatcg cctccacaat
      301 cggcggcgtt cccgcctcga acctcgtggg cggcgggccg taagtgatgc catcgacggt
      361 cacgctctcg atcatcgaac caccgcccaa gaagggcggc agcgattcca gcaggctctt
      421 gcgaccccag agcactccga taccggacgg cccgtaaacc ttgtggccgg tgaacgcgta
      481 gaagtcgcag ccgatgtcct gcacatcgac ggggagatga accgcccctt gcgagccgtc
      541 gacgagcacc ggaatgccgc gttcgtgcgc aatccggcag acctctttca ctggaacgac
      601 cgtgcccagc acgttggaaa cgtgggtcac cgcgacgatc ttggtgcggg ggcccagcaa
      661 ccgctcgaat tcatcgagca ggaactcacc ggtgtccgaa atcggcaccc acttcagcac
      721 cgcccctttg cgctcgcgga ggaagttcca gggaacgatg ttcgagtggt gctccatgat
      781 ggagagcacg atctcatcgc cttcctggat gttctcgagg ccgaaggaat acgcgacgag
      841 gttgatcgac tccgtcgcgt tcttcgtgaa aacaatctcc tcggcggaag gcgcattgag
      901 gaagcgccgc actgtctcgc gcgcatcctc atacttttgc gtggccgtgt tcgagagcag
      961 atggatcccg cgatgcacgt tcgcgtattc gaacctgagc gcgtgatcca ttgcctcgat
     1021 caccgacagc ggcttctgcg ccgatgctgc gctgtcgagg tatacgagcg gtttgccgta
     1081 aacctcgcgg aacaggatcg ggaagtcggc gcgatagcgc tcgacctcaa accgcctacc
     1141 ggcttcgaat gccgctgcct gtgtcacgtg cctgccggct cttaatgagc cgtcctctgg
     1201 ttcagccaca cgcgagccgt ttccatcagc gcctcgcgca cgggctcatc ctcgaccttc
     1261 tccatcacct cgccgacaaa ggcttcgatg aggagcctac gcgcctcggg ttccggaatg
     1321 ccgcgcgccc tgcagtagaa gatgtgctct gggtcgatgt cggccgccgt ggatccatgg
     1381 ccgcagacga cgtcgtcggc ataaatctca agttccggct tgctgtcgaa ctcggcatcg
     1441 ggcgacagca tcagcgcctg cgccatctgc ttgccgtccg tcttctgcgc gcccggcctg
     1501 acgatcacct tgccctggaa gacgccgcga ccgtgaccat cgagcacacc tttgaaaagc
     1561 tcgcggctct cgcaatgggg caccgcatgg tcgacgagca aagttgtgtc gatgtgctcg
     1621 ttgttggtcg ccaggaacga gcccgagcag tcgagcttgc cgccttcgcc agcgaacttg
     1681 acgaagatct gattgcgcgc gagccccggc cctgccgtga gctggaagat ccggtaggtc
     1741 gcgttggcac cgatttccgt ggtggcactg ccgaggtgca cggccctgcc attctccacg
     1801 atcgccttga cgtgggttac ggcagcgccc tcgcccacca cgacctccgt cgcggcgttg
     1861 tgctggtcct catcggccgc tcctgccaga ggcacgtaag cctccaccag cgtcaccttg
     1921 gagcctttgc cgatgctgat gacgttgcgg gtcgtgatcg tcttgcgatc agcaccggcg
     1981 cgggcgaata ccagcagcag cggcttgtcg agctccgtgc cgtcagcaat gcgcaccacc
     2041 gccccgtccg tcgcgaaggc gctgttcagg gcctcgatcg catcctgatc gcgcttcatg
     2101 cgcacgatat caggcgattc tgccagcacc tcgctgagcg gcttcaccga cagcccgtcc
     2161 ggcagctgtg cggtagaaag gccggcattg aaagccccgt cgacgaccac gacgcgatag
     2221 gtatcgaggg cccccagcgg ccccagcccc gcattgaggt ctgcctcgct cggcgctgcc
     2281 gccttgccga tggccggcgc aaaaacctcc ttcagcaagc tgcgaaggtc ggtgtatttc
     2341 cattcctcga tgcgccgatg cggcaagccc agcgccgcaa acgtgccaat ggctttccgg
     2401 cgcagctcac ggacggcctc gctccccggg agcttcgcgg caaccgcctc gaaggtctcc
     2461 gtgaaggcct gctcggcctt cgttttggtc aagcccgcgt tcatctcgaa atcacccgct
     2521 tgcttcaggc cgccttcgtg aactcggcgt agcccgaccg ctcgagctcg accgccagct
     2581 ccttgtcccc cgtccgctgg atgcggccgt cggcgaggac gtgcaccttg tcgggaacga
     2641 tgtagttcag taggcgctgg tagtgcgtga tgaccaccat cgcacgatcc gggctacgca
     2701 gcgcgttgac gccatcggcc acgatccgca gcgcgtcgat gtcgaggccg gagtcggtct
     2761 cgtccagcac gcagagcgtc ggctcgagga gcgccatctg caggatctcg gaccgcttct
     2821 tctcaccgcc cgagaagccg acgttcaccg gacgcttcag catttcgacg tcgatgttga
     2881 gcttggccgc gcgctcgcgc accagcttca tgaactccgg cgtagtgagc tcaggctgct
     2941 cgcgccgctt acgcagcgcg ttgagtgccg tgcgcaggaa ggtgagaccg gccacaccgg
     3001 ggatctcgat cgggtactgg aaggcgagaa atacgccctt caccgcccgc tcgtcgggct
     3061 ccatttccag caggttctca ccattccaaa ggatctcacc ctcggtcacc tcgtaatcct
     3121 cgcggccggc gagaacgtag gcgagcgtgg atttgcccga tccattgggc cccatgatcg
     3181 cgtgcacctc ccccttcggt acgatgaggt tcacgccctt gaggatcatg gtgtcctcgt
     3241 cagcgatctt tacgtgcaaa ttccggatct caagcatctc cacgtctctc cttgaggcgc
     3301 cttctggcgc ccacttccaa cacctacccg gctcacgcgt acgcggagcc ggaatctttt
     3361 ccattccctt gcgcagccca gtagcgatcg aacgcagcgc tggccgcccg ctccgcttcc
     3421 tcctggcgag ccttgagctg ctccaccatc gccacgagcg cgctcaaatc cgctccattt
     3481 gctccggcgg actgccgcat tcgcgccagc tccgcgcttg ctttcgcggc ttcgagcttc
     3541 agggcatcgg ctgctgtttc ggcttcgagc caagcctgct caagcagggc aatttccgtc
     3601 gacatccttt cgtttcctcc gcttacaacc gaggcttggc tctcgtcctt atggccccga
     3661 tgctaccctc ttgctcgtgc cccacgtctt tgtgccggcc caaggggccg gcgccgttct
     3721 cgttagccga ccgatccttc caggctgatg ccgatcagct tctgcgtctc cgccatgaac
     3781 tccatcggca gctgctgaat gacgtcgcgg acgaagccgt tgacgatgag cgccacggcc
     3841 tcctcctccg acaggccccg ctgcatgcag tagaagagct ggttgtcgga gatcttcgac
     3901 gtcgtcgcct cgtgctcgaa gtgcgccgtc gagttcttgg cctcgatgta cggcaccgtg
     3961 tgcgcgccgc acttgtcgcc gatcagcaag ctgtcgcagt tggtgaagtt gcgcgcgttc
     4021 gtcgccttgc ggtgcgctga gacaaggccg cggtaggtgt tctgcgagta ccccgccgcg
     4081 atgcccttcg agatgatccg gctcgacgtg ttcttgccga ggtggatcat cttggtgccg
     4141 ctgtcgacct gctggtagcc gttcgaaatc gcgatcgagt agaactcgcc gcgcgagccg
     4201 tcgccgcgca ggatgcagct cggatacttc caggtgatgg ccgagccggt ctcaacctgc
     4261 gtccacgaga tcttcgacga gtggccgcgg cagtcgccac gcttggtgac gaaattgtag
     4321 atgccgccct tgccctcgga gttgccggga taccagttct gcaccgtcga atatttgatc
     4381 tcggcatcgt cgagcgtgac gagttcgacg acggcggcat gcaactggtt ctcgtcccgc
     4441 tgcggcgcgg tgcagccctc gagatagctg acgtaagcgc ccttgtcggc gatgatcagc
     4501 gtgcgctcga actggccggt gttgcgctcg ttgat
//
LOCUS       DASUMG010000411         6047 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_775722, whole genome shotgun sequence.
ACCESSION   DASUMG010000411 DASUMG010000000
VERSION     DASUMG010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6047
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775722"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="VFX46_06800"
     CDS             complement(<1..1232)
                     /locus_tag="VFX46_06800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX5600324.1"
                     /translation="MQPDDGRNVSVRLQRDAALKAVRSNGEFDVIVLGGGINGIGVFR
                     ELSLQNLRVLLVERRDFCSGCSSAPSRMIHGGLRYLENGEFGLVRESLRERDALLRNA
                     PHMVRPLPTTIPIRKVFSGMLNGVAGFLGLSSRPSSRGALPIKIGLMLYDWNTRHRRL
                     LPRHKFRGARETLARWPKLNPHLRFSATYFDAWISYPERLCIELLLDTARMAPRSVAL
                     NYAEIKRSGDGFLLADRTNGETMEVRARAIVNATGAWLDEARASLANGKPPQERFVAG
                     TKGSHLIIDNAELREALGGHMIFFENEDGRVCILFPYLGKVLAGATDIRVDTPDRVRC
                     EPEEREYILGAIREVFPDIRVREEEIVFSFSGIRPLPKSDHEFTGRIPRSHYIRREDG
                     PVPQFCMIGGKWTTFRAFA"
     gene            complement(1238..2236)
                     /locus_tag="VFX46_06805"
     CDS             complement(1238..2236)
                     /locus_tag="VFX46_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019564981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX5600325.1"
                     /translation="METHEMLTREIGRSGIRASAIGLGTWAIGGWMWGGTDEREAIGA
                     IRASLDEGVTLIDTAPAYGQGLAEEIVGKAIRGRRDKVVLATKCGLVWHTTKGRHFFD
                     YDGRPVHRYLGRDAIFYEIEQSLRRLGTDYIDHYITHWQDPTTPIEETVGALEELKRQ
                     GKIRSIGASNVSPEELKAYIATGQLDAIQEEYSMIERRIEKALLPICAEHGVSVLSYS
                     TLALGLLSGKIGPDRQFTGDDQRKNHPRFSVANREKVARFMAEIAPIAKAHGATEAQI
                     VIAWTLQQPGITFALCGARNPRQAAENAKAGRLRLASDELAQIDAAIARHLTGLDA"
     gene            complement(2244..3224)
                     /locus_tag="VFX46_06810"
     CDS             complement(2244..3224)
                     /locus_tag="VFX46_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEX5600326.1"
                     /translation="MADRKDDFEAIRQIYTVLMLHFIEGMKQNEISQKMNLSPSKVNR
                     LISQGKKLGMVKISIESPFQSLVELERKLKGIGRLSDAVVTPTVSGSQETTLQQVGRA
                     AGNQLLESLRDGDVIAITGGKAVSAVVENLRPERQFDVTVVPLTGGVQGKFYTDVNHL
                     ASRLAEKLGGRTMLLHAPLFAESATQRDLLREMAPVREVFNLGRKAAIALVGIGSVLT
                     PGSSYYDLHPLPDLDPEELLRKGICGEFLAHLICADGQVADHPLNERLVALSPAEIAQ
                     CRRVIGVAAGPAKVQPILAALRGRFLHTLVTDEETAARALEALEPVRNVA"
     gene            complement(3227..4150)
                     /locus_tag="VFX46_06815"
     CDS             complement(3227..4150)
                     /locus_tag="VFX46_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600327.1"
                     /translation="MRETGLILIILVLFAVMSFASPYFLTWNNMRAMAMAFAVEGIVV
                     VGMTILLISGGIDLSVGSVTALAMVIAGLLFLSGFDPWIASLISIAACTAIGATMGFF
                     VTKVGLHHFIVSLAVMVIARGLCLLGTGGRPLGLYTLPPEFKFIGQGSVGPIPVVIII
                     FVVVVVVFDFLLRRTTMFRKVFYTGSNEKAAAYSGIRTKKVIFLTTTLSSALCGVAGV
                     IYMARFGSAQPTFGIGMELNVIAAAVIGGASLSGGTGTIFGAILGTVLLSVVSSSLVL
                     LNVSVYWQDIIRGSILLSAVTIDHYINKRRG"
     gene            complement(4249..5781)
                     /locus_tag="VFX46_06820"
     CDS             complement(4249..5781)
                     /locus_tag="VFX46_06820"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX5600328.1"
                     /translation="MPALPTEEPVLEIRKLSKSFGPIWALHGVDFELRRGEIHALAGE
                     NGAGKSTLVNIIDGILRPDEGEIWLFGEPVNITSPAVAQKYGIGFVHQEIALCPDISV
                     AENIFMCRTATSRSFFTDARALEREAAEILRDLGDIDPGRKVGSLSISQQQLVEIAKA
                     LTLDCRILILDEPTAALTEREAQLLFRIMRRLAARGISIIYISHRMAEIFSNCDRVTV
                     LRDGQYITTLNIPETTPDEVVNAMVGRVIGNYYPTKLAPYEQSPEPILEVRGLSERRR
                     FRDISFELRRGEILGFAGLIGAGRSEIARGICRLEGEVTGEIRLHGRRVKVSSYADSI
                     REGIVYLSEDRKGDGLFLDLPITANVSALRVEQVANAFGIIDEHKEWEQAETLGKRLG
                     LKCDHVGQPVSALSGGNQQKVALAKMLSVDPKVIFLDEPTRGVDVGAKSEIHRILRNL
                     AQNGVGIVVISSELPELIGVCDRILVIREGRLVGEVSGDEMTESRIMYLASMGEPGRL
                     AS"
     gene            complement(5834..>6047)
                     /locus_tag="VFX46_06825"
     CDS             complement(5834..>6047)
                     /locus_tag="VFX46_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX5600329.1"
                     /translation="NPNQGMQGFMGFIATFLSAHPDMFDPFNDYKISGYNPVQIPFID
                     NGFAVITKENADAFDLNKYMEGRDPS"
BASE COUNT         1199 a         1865 c         1803 g         1180 t
ORIGIN      
        1 gcaaaggcgc ggaacgtcgt ccactttccc ccaatcatgc agaactgcgg caccggcccg
       61 tcctcgcgcc ggatgtagtg gctccgggga atgcggcccg tgaactcgtg atcgctcttc
      121 ggcaaaggac ggatgccgct gaagctgaaa acgatctcct cctcccgcac gcggatgtcg
      181 ggaaaaactt cccggatcgc cccgagaatg tattcgcgct cctccggctc gcaccgcacg
      241 cggtccggcg tgtcgacgcg aatatccgtc gccccggcca gaaccttgcc gagataggga
      301 aagaggatgc agacccgccc gtcctcattc tcgaagaaga tcatgtgccc gccgagcgcc
      361 tcgcggagtt ccgcattgtc gatgatgaga tgcgagccct tggtcccggc gacgaacctc
      421 tcctgcggcg gcttcccgtt cgcaagactg gccctcgcct cgtcgagcca ggcccctgtg
      481 gcgttgacga tggcgcgcgc gcgcacctcc atcgtttcgc cgttcgtccg gtcggctagc
      541 agaaatccgt cgcccgaacg tttgatttca gcgtagttca gcgccaccga acgcggtgcc
      601 atgcgcgcag tatcgaggag aagctcgatg cagagccgct cgggatagga gatccacgca
      661 tcgaaatagg tggcagaaaa acgcaggtgc ggattgagct tcggccagcg cgcaagcgtc
      721 tcgcgtgccc cgcggaattt gtgtcttggc aggagtcggc ggtgacgcgt gttccagtca
      781 tacagcatga gcccgatttt gatcggcagt gcgccgcggc tggacggacg gctcgacagg
      841 ccgagaaagc ccgccactcc gttgagcatg ccggagaaaa ctttgcggat cggaatggtg
      901 gttggcagcg gccggaccat atgcggcgca ttccgcaaca gcgcgtctcg ctcgcgtagg
      961 gactcgcgca caagcccgaa ctcgccgttt tccaaatagc gcagccctcc atggatcatg
     1021 cgcgacggcg cggaactgca gcccgagcag aaatcccgcc gctccacgag caacactctt
     1081 aggttctgca gcgagagctc gcgaaaaaca ccgattccgt taatgccgcc acccagcacg
     1141 atgacatcga actcgccatt gctgcgcacc gctttgaggg cggcgtcgcg ctgcaggcgg
     1201 acagaaacgt tgcggccatc atccggctgc atcgcgttca cgcgtcgagc ccggtcagat
     1261 ggcgcgcaat ggcggcgtcg atctgcgcga gctcatccga tgcgaggcgg aggcggccgg
     1321 ctttcgcgtt ctccgctgct tggcgcggat tgcgcgcgcc gcacagcgcg aaggtgatgc
     1381 cgggctgctg gagcgtccag gcgatgacga tctgtgcctc ggttgcacca tgggccttgg
     1441 cgatcggcgc gatctccgcc atgaagcgag cgactttctc gcgattggcg acggaaaagc
     1501 gcggatggtt ctttcgctga tcatccccgg tgaactgccg gtccggaccg atttttcccg
     1561 agagcagacc cagtgccagc gtcgaatagg acaagaccga gacgccatgc tccgcacaaa
     1621 tcggcaggag cgctttctcg atgcgccgtt cgatcatgct gtattcttcc tgaatggcgt
     1681 cgagctggcc agttgcgata taggccttga gctcttccgg cgagacgttg ctcgcgccga
     1741 tcgaccggat cttgccttgc cgcttcagct cctcgagcgc gccaactgtc tcctcgatgg
     1801 gcgtggtcgg gtcctgccag tgcgtgatgt aatggtcgat gtagtcggtc ccgagccgcc
     1861 tgaggctttg ctcgatttca tagaaaatcg catcgcgtcc gagatagcgg tgcaccggac
     1921 ggccatcata atcgaagaaa tgcctgcctt tcgtcgtatg ccagacgagg ccacatttgg
     1981 ttgccagcac gaccttatcg cgccgaccgc ggatcgcctt cccgacgatc tcctcggcaa
     2041 ggccctgacc ataagccggc gcggtgtcga ttagggtaac gccttcatcc agcgaggccc
     2101 taatggcgcc gatcgcctcg cgctcgtccg tgccgcccca catccagccg ccgatggccc
     2161 aagtcccaag gccgattgcg gaagcacgaa taccggaacg gccgatctcg cgggtcagca
     2221 tttcatgtgt ttccatctcg gtttcaggcg acattgcgta cgggctccag tgcttcgagg
     2281 gcgcgagccg ctgtctcctc atcggtgacc agcgtgtgaa ggaagcgacc gcgcagggca
     2341 gccaggatcg gctggacttt cgcaggccca gccgcaacac cgatgactcg ccggcactgc
     2401 gcaatttcag caggcgacag ggcgaccaat ctttcgttca gggggtgatc cgctacttgg
     2461 ccgtcggcac aaatgagatg ggcaaggaat tcgccgcaga tacccttgcg gaggagctcc
     2521 tccgggtcga gatccgggag cggatgcagg tcgtaatagc ttgatcccgg cgtgagaacg
     2581 gatccgatgc cgacgagcgc aatcgccgcc tttcggccaa ggttgaatac ttcgcgcacc
     2641 ggcgccatct cgcgaagcag atcgcgttga gttgcgcttt cggcaaacag cggcgcgtgc
     2701 agcagcatcg tcctcccgcc cagcttttcc gcaagccggg aagcgagatg attgacgtcg
     2761 gtgtagaact tcccttgcac gccccctgtc aggggcacga cggtcacatc gaattggcgc
     2821 tcggggcgca aattttctac caccgcgctt acagccttgc cgccggtaat ggcgattaca
     2881 tccccatcgc gcagcgattc cagcagctgg ttgccggcag cgcgaccgac ctgctgcagc
     2941 gtcgtttctt ggctgccgga cacggtcggc gttacgactg catcggagag cctgccgata
     3001 cctttgagtt tccgctcaag ctccacaagg ctctgaaacg gactttcgat agagattttt
     3061 accatcccaa gtttttttcc ctgggatatc agtctattca ccttagacgg cgacagattc
     3121 attttctggg aaatctcgtt ttgcttcatc ccctcgataa aatggagcat aagcaccgtg
     3181 taaatctgcc ttatggcctc gaaatcgtct tttctgtctg ccactttcag ccacgccgct
     3241 tgttgatgta atggtcgatt gtcacggccg acagcagaat cgagccgcgg atgatgtcct
     3301 gccagtagac ggagacgttg agcagcacca gtgagctcga cacgacggac aggagcacgg
     3361 tgccgagaat ggcgccgaaa atcgtgcccg taccgccgga aaggctggct ccgccgatga
     3421 ccgccgcggc gatcacgttc agttccatgc cgattccgaa agtgggctgc gctgaaccga
     3481 aacgggccat ataaatgaca cccgctactc cgcacagcgc cgagctgagc gtcgtcgtca
     3541 ggaaaataac tttcttggtg cggataccgg aataggcggc tgccttttcg ttgctgcctg
     3601 tatagaacac tttgcggaac atggtcgtgc gccgtagcag aaaatcgaag accaccacca
     3661 cgacgacgaa gatgatgatc accaccggaa tggggccgac cgatccctga ccaatgaact
     3721 tgaattcagg cggcagcgta tagagtccca gcggcctgcc accggtacct agtaaacaaa
     3781 gcccgcgcgc gatcaccatc actgcgagcg acacgatgaa gtggtgcagg ccgaccttcg
     3841 taacgaagaa gcccatggtg gcgccgatgg cggtgcaggc ggcaatggaa atgagggacg
     3901 caatccaagg atcgaagccg ctcaggaaca gaagaccagc gattaccatc gccagcgccg
     3961 tgactgaacc gaccgagagg tcgatgccgc ccgaaatgag aaggatcgtc atgccgacca
     4021 cgacgatgcc ctcgaccgcg aaggccatcg ccatggcccg catgttattc caggtcagaa
     4081 aatagggcga ggcgaaggac attaccgcga acagcaccag gatgatcagg atcagcccag
     4141 tttctcgcat gccgagtagg cgcttcagcg aatgggagag cccgcttgca gccgtaagcc
     4201 cgcttcgctt gtccgcgcca gcagccgctc cgatggaaac atcgctcttc atgatgccaa
     4261 tcgccccggt tcgcccattg aggcgagata catgatgcgg ctttccgtca tttcgtcgcc
     4321 gctgacctcg ccgacaagcc gtccttcccg tattaccagt atcctgtcgc agacgccgat
     4381 cagctccggc agttcagaag aaatcacgac gatgccgacg ccgttctgcg caaggttccg
     4441 cagaatgcga tgaatttcgg actttgcacc gacatcgacg ccccgcgtcg gctcatcgag
     4501 aaagataact ttaggatcga ccgacaacat ctttgccagg gcgaccttct gctggttgcc
     4561 gccggacagc gccgagaccg gctgcccgac gtggtcgcat ttgaggccga gccgtttgcc
     4621 gagggtttcg gcctgctccc actccttgtg ttcgtcgatg atgccgaatg cgttcgccac
     4681 ctgctcgaca cgcagggcag aaacattggc ggtgatggga agatcgagaa acagcccgtc
     4741 gccctttcga tcctccgaaa ggtaaacaat tccttcgcga atgctgtcgg cgtagctact
     4801 caccttgacc cgtcgcccat gcagccggat ctcgccggtc acctcgccct cgagccggca
     4861 gatgcctcgg gcgatttcgc tccgccctgc cccgatcagc ccagcgaagc cgaggatttc
     4921 gcctcgccgc aattcgaagg agatatcgcg gaagcgccgc ctctcgctga gcccccgcac
     4981 ctcgagaatt ggttcaggcg attgttcata gggagccagc ttcgtcggat agtaattgcc
     5041 gatgacgcgg cccaccatgg cattcacaac ttcgtcgggc gtcgtttcgg ggatgttcag
     5101 cgttgtgatg tactggccat cacgcaacac ggtcacccgg tcgcaattgc tgaagatctc
     5161 ggccatgcga tgcgatatgt agataatgga aattccccgt gccgcgagcc gccgcatgat
     5221 gcggaagaga agctgggctt cacgctcggt cagtgctgcg gtcggctcgt ccaggatgag
     5281 gatgcggcag tcgagcgtaa gcgctttggc gatctcgacc agctgctgct gcgagatgga
     5341 cagcgagccg actttcctgc ccggatcgat gtcgccgaga tccctaagga tttccgccgc
     5401 ctcccgttcc agcgcgcgcg catctgtgaa aaagctgcgg ctggtcgcgg tgcggcacat
     5461 gaagatgttt tccgccacag agatatcggg acagagggca atttcctgat ggacaaaacc
     5521 gatgccgtat ttctgggcga cggcaggcga ggtgatattc accggctcgc cgaaaagcca
     5581 gatttcgcct tcgtccggcc gaagtatgcc gtcgatgata ttgactagcg tcgacttgcc
     5641 cgccccgttc tctccggcga gcgcatggat ttcgccgcgc ctgagctcga agtcgacccc
     5701 gtgcagggcc caaattggcc cgaacgactt cgagagcttt ctaatctcga ggacaggctc
     5761 ctcggttggc agtgcgggca tttcctctcc gtgcggaagc aatgcccgcg agcggggcat
     5821 cgcacgcgcc acctcagctc ggatcacggc cttccatgta tttgttgagg tcgaaggcgt
     5881 cggcgttttc cttcgtgatc acggcgaagc cgttgtcgat aaagggaatt tgcaccgggt
     5941 tgtagcccga gatcttgtag tcgttgaagg gatcgaacat gtccggatgg gcggatagga
     6001 aggtggcgat gaatcccatg aagccctgca tgccctgatt gggattg
//
LOCUS       DASUMG010000412         3353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1177821, whole genome shotgun sequence.
ACCESSION   DASUMG010000412 DASUMG010000000
VERSION     DASUMG010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177821"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1057
                     /locus_tag="VFX46_06830"
     CDS             38..1057
                     /locus_tag="VFX46_06830"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX5600330.1"
                     /translation="MAAGRRAAVLLSLGLLILPTAGASEQGGGATQPERRVSQVHQDH
                     ALVGTIWSAREGRVVSRDELLDALSTTSFVLLGEVHDNPEHHSLRAWVLGELASRRKA
                     SGEDGPAVVFEHIRADQQSVVDDFLGSADAADAEALLQKLEWDRSGWPPAAMFSPLFE
                     QALKFRLPILGGNTPVDAIRRVAREGLATLPEEERSRLGLDRQLEPPLRTALIKEIEE
                     NHCGLLPESAFAPMALAQQYRDAHLAKKMSDAMEGIGAVVLLAGNGHVRADRGVPWHL
                     RRRGPGARIGVVAFVEVDPSMTETETYVPRDPAGQPAVDYVWITSRAERPDPCEQMRQ
                     RYSRP"
     gene            1362..2537
                     /locus_tag="VFX46_06835"
     CDS             1362..2537
                     /locus_tag="VFX46_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEX5600331.1"
                     /translation="MERFPSAAALIAHSKPDRPVLASRPHAAGRAARWFLENFPGEVA
                     YAYKANSSVFLLGALFGAGIRQFDVASLPELEDAATIPGAKLHFMHPVKSRTAIRRAY
                     FEFGVKCFALDTEDELAKIVEETGGAKDLELFVRVAVPAKNSRIPLERKFGVSGQKAA
                     RLLMKTRQVADELGITFHVGSQTTTPHAFVTALEEVHKLIVKAGVVVDAIDIGGGFPS
                     RYSDSEPAPLSAFIEAIRKGFEKLPVSEACRLICEPGRALVAEAESLIVQVDARKGND
                     LYINDGGYGALFDSAHLGFVFPVRLVSREPAKGERMVPFSMWGPTCDSIDFMKGPFFL
                     PESVREGDYIEIGNIGAYGRAIASNFNGYGRYDEVILLDEPMYTMYGNAKQQVAQHA"
     gene            2615..>3353
                     /locus_tag="VFX46_06840"
     CDS             2615..>3353
                     /locus_tag="VFX46_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX5600332.1"
                     /translation="MTDFRSGEFDYLPASEAFLEPDRADADRPHEARAVVIPFGLEAS
                     VSYGTGTAAGPAAILAASHQLELFDEELWREPYLDFGIATLRPPVIEKPLAAALEQIA
                     GIVGSVLGQNRFPFVLGGEHSLTAGAIRPFAAAYPDLVVLQFDAHADLRDGYLGEHYS
                     HASAMRRVLDHEHVTLVSVGIRAISSGEVAYLEANRHRIHIHWAKDQAQWDIEEIVKP
                     LRGRPVYVTFDIDGLDSACMPATGTPTP"
BASE COUNT          585 a         1016 c         1103 g          649 t
ORIGIN      
        1 gaacgcaagg gcctgccgcc ggtcgaggag tgctaggatg gccgcgggcc gccgcgcagc
       61 tgtgctgctg agtttgggcc tgctaattct tccgacggcg ggtgccagcg agcagggcgg
      121 gggtgcaacc cagccggaga ggcgagtgtc gcaggtgcac caggatcacg cgctggtagg
      181 tacgatctgg tcggctcgag aaggccgtgt cgtctcgcga gacgagctgc tcgatgcgtt
      241 gtcgaccaca tctttcgttc tgctgggcga ggtccatgac aatcccgagc accacagcct
      301 gcgcgcttgg gtacttggcg agctcgcgag ccgccgaaag gcttcgggag aggatggtcc
      361 agctgtcgtt ttcgagcata tccgcgccga tcagcaaagc gtagtcgatg actttctcgg
      421 ttctgccgat gcagctgacg cagaggcgct gcttcagaag ctcgaatggg acaggagcgg
      481 ctggccgccg gctgcaatgt tttccccgct gttcgaacag gcgttgaagt ttcggttgcc
      541 cattttgggg ggcaacacac cggtggacgc gatccgcagg gtcgcacgtg aagggctggc
      601 gactctcccg gaggaggagc gatcccgcct tgggcttgat cggcaactcg agccaccgct
      661 gaggactgcc ctcataaagg aaatcgagga aaaccattgc ggattgctgc ccgaaagcgc
      721 attcgcgccg atggcgctcg cccagcagta ccgcgatgcg catcttgcca agaagatgtc
      781 tgacgcgatg gaagggatcg gcgcagtggt gctgctggcg ggcaacggtc atgtccgcgc
      841 ggaccgtgga gttccctggc atctgcgccg ccggggcccc ggcgctcgta tcggtgtcgt
      901 cgccttcgtg gaagtggacc cgagcatgac agagaccgag acctacgtgc cacgcgatcc
      961 tgctgggcag ccagccgtgg attacgtatg gatcacgtcg cgagccgagc gacccgatcc
     1021 gtgcgagcaa atgcggcagc gctattcgcg cccctgaggc tgagccgtcg atcgggcgcc
     1081 ggtcttcaca ggtctggccg caagtaggac ctcccgataa tcgatcctat atagccttat
     1141 agtcttccgc gccattgcga ttgtttgacc ggcaactctg ctcgtgcgtc ttttcgggcg
     1201 aggcggcaga aaaatgcgcg ccgagctctt caacgccctt tacatgccga gcaaccgatc
     1261 ttatttcggc gactcattcg aatacttcag aagcggcgat ctctgctggt tcagcaaggc
     1321 gccatctggt ccgtttttcc gactggtgga ggtcccatgg aatggaaaga tttccgtctg
     1381 cggcggcgct gatcgcccac tccaagcccg atcgtccggt gcttgcctcc cgtccgcatg
     1441 ccgcgggccg ggccgcacga tggttcctcg agaactttcc cggcgaggtg gcgtacgcct
     1501 acaaagccaa cagctccgtg tttctgctcg gcgcgctttt cggcgcgggg atccgtcaat
     1561 tcgacgtcgc ctcgctgccg gagctggagg atgctgccac catccccggc gccaagctgc
     1621 atttcatgca cccggtgaag tcgcgaacag cgattcggcg cgcctacttc gagttcggag
     1681 taaagtgctt cgcgctcgac acggaggatg agctcgcgaa gatcgttgag gagaccggtg
     1741 gcgccaagga tcttgagctt ttcgtgcgcg tggccgtgcc ggcgaagaac agccggattc
     1801 cgctggagcg gaagttcggc gtttctgggc agaaggctgc ccgcctactc atgaagaccc
     1861 gccaggtggc cgacgagctc ggtatcacct tccacgtcgg ctcgcagacg acgacgccgc
     1921 acgcgttcgt cacggcgctg gaggaggtcc acaagctcat cgtcaaggcg ggggtggtcg
     1981 tcgacgctat cgatatcggc ggcggcttcc cctctcgcta tagcgatagc gagccggcgc
     2041 cactgtccgc gttcatcgag gcgatccgga aggggttcga gaaactgccg gtgagcgagg
     2101 cgtgccgtct catctgcgag ccgggccggg cgctggttgc agaggccgag agcctgatcg
     2161 tgcaggtcga tgcgcgcaag ggcaacgatc tgtacatcaa cgacggcggc tacggcgcgc
     2221 tgttcgactc ggctcaccta ggattcgtgt tcccggttcg gctcgtcagc cgcgagccgg
     2281 cgaagggcga gcggatggtg ccgttctcca tgtgggggcc gacctgcgac tcgatcgatt
     2341 tcatgaaggg gcctttcttc ctgccggagt cggtccgcga gggggactac attgaaatcg
     2401 gcaacattgg cgcctacggc cgcgccatcg ccagcaactt caatggctat ggccgctacg
     2461 acgaggtgat ccttttggac gagccgatgt acacgatgta cggcaacgcc aagcagcagg
     2521 tggctcagca cgcctgactt cagcgcggag tgctcatgcc cctctttccc tcgcgggaag
     2581 aggggttttc tttataggga caacagacgg aaacatgacg gattttcgtt cgggcgagtt
     2641 cgattacctc cctgccagcg aggcctttct cgagccggac cgagcagacg cggacaggcc
     2701 acacgaggcg cgcgccgtcg tcatcccgtt cgggctagaa gcgtccgtga gctacggtac
     2761 ggggacagca gcgggaccgg ctgccatcct cgcggcgagt caccagctcg agctgttcga
     2821 cgaagagctg tggcgtgagc cgtatctcga tttcggtatc gccacgttgc gtccgcctgt
     2881 aatcgagaag ccgctcgcgg cagccctgga gcagattgcc gggatcgtgg ggtcagtcct
     2941 ggggcaaaac cggttcccct ttgtccttgg tggggagcat tcgctcacgg cgggcgcaat
     3001 tcgtcctttc gcagcggcgt atccagatct ggtggtgctc cagttcgatg cgcatgcgga
     3061 cctgcgcgac ggctacctcg gcgagcacta ttcccacgcc tcggccatgc gccgggtgct
     3121 tgatcatgag cacgtgacac tggtctccgt cggcatccgc gcgatctcct cgggtgaggt
     3181 cgcttatttg gaagccaacc ggcatcgcat tcacattcac tgggccaagg atcaggccca
     3241 atgggacatc gaggaaatcg tgaagcccct gcgcggcagg ccggtgtacg tcacgttcga
     3301 cattgacggt ctggatagcg cgtgcatgcc tgcaacgggg acgccaactc ccg
//
LOCUS       DASUMG010000413         3347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_615160, whole genome shotgun sequence.
ACCESSION   DASUMG010000413 DASUMG010000000
VERSION     DASUMG010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615160"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VFX46_06845"
     CDS             complement(<1..558)
                     /locus_tag="VFX46_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600333.1"
                     /translation="MLGYIIRRILAVIPVIVSVAIFVFLLLRLTPGDPAAIIAGDMAT
                     PEQLERIRRSLGLTEPLHVQFIRWVGLLLQGDLGTSLISNTPVTRMIGQRIWPTINIA
                     LLTITLSVLIAVPLGVLAAWRHRTWADYAVMTFSVLGFSIPVFVIGYIFIQIFSIELR
                     WLPVQGYSPPSQGFGRFLSRAILPCL"
     gene            complement(657..2198)
                     /locus_tag="VFX46_06850"
     CDS             complement(657..2198)
                     /locus_tag="VFX46_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600334.1"
                     /translation="MLAGTSLLQPAAAEKVITGVMHSGLRVLDPIITTAHITRDHAYM
                     IYDVLVAVDENFQPRPQMADWTISDDKLVYTFKLRDGLKFHDGNPVTAKDAVASLKRW
                     GKRDSGGQLIFDVTESLEAADDKTIVWKLKKPFPALLEIVGKQSAVPPFIMPERVANT
                     PPDQAITEYIGSGPFVFVESEYQPGVSVTYEKFADYVPREEPPSWMAGGKVVKVDKVK
                     WVTMPDAQTTINALLSGEIDYIEQVQIDLLPLLEGNDDVVVETRDDLGYQTMGRMNFK
                     HPPFDNVKIRQAALKALNQADVLGTMIGDPKYYKICGAIFGCGTPLGDESGAETLIKG
                     GDKEGAKKLLKEAGYDGTPVVLMQPTDVISLQKQPVVAAQALREVGFNVDMQPMDWQT
                     LVTRRASQAKPSEGGWNLFFTNWMVPEINSPLISPMLNGRGDQGWFGWPKDDELEALR
                     AEFIEADTPEKQREVAVRIQKHVLENVIYIPLGQYAAPQARSKKLTGMIPSPVPVFWN
                     IEKAD"
     gene            complement(2380..>3347)
                     /locus_tag="VFX46_06855"
     CDS             complement(2380..>3347)
                     /locus_tag="VFX46_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MlrC C-terminal domain-containing protein"
                     /protein_id="HEX5600335.1"
                     /translation="MARGRPFAKAFRRLPFLIPIPWQCTSLEPARSIYQRVAELEAGE
                     VFSTSFLMGFPASDFEECAPSLLAYGETQAAADKTANEMMELVLANELAFKGQAYDPD
                     EGVQIAMKLAEKATRPVVIADTQDNPGAGGDSDTMGLLRALLRNGAKRAAIGLIADAE
                     AARAAHQAGEGAEISIALGGKSRVPGDSPLEASFRVEKLSNGQLHATGPYYGGTHLNL
                     GPSACLAIDGVRIVVTTHKAQMADQAMYRFVGIEPREQAILVNKSSVHFRADFEPIAE
                     AILVCTAPGPMPLDPATLPWQKLAEGIRLSPGGPEFRRSARSPAS"
BASE COUNT          666 a          980 c         1022 g          679 t
ORIGIN      
        1 caggcagggc aggattgcgc gggaaagaaa acgtccgaat ccttgcgatg gcggagagta
       61 tccctggaca ggcaaccagc gcaattcgat cgaaaagatt tggatgaaga tatagccgat
      121 gacgaaaacg gggatcgaaa aaccaagcac cgagaatgtc atgacggcgt agtcggccca
      181 ggtgcggtgc cgccacgcgg ccagcacgcc cagcggaacg gcaatcaaaa cagagagcgt
      241 gatcgtcagc aacgcgatgt tgatcgtcgg ccagatgcgc tgcccgatca tacgcgtcac
      301 gggagtattg gaaataagtg atgtcccgag atcaccttgg agcagaagtc caacccacct
      361 tatgaactgc acgtgaagcg gctcagtgag gccaagagag cggcgaatgc gttcgagctg
      421 ctccggagtc gccatgtctc cggcaatgat cgctgcaggg tcacccggtg tgaggcggag
      481 aagcaagaat acgaaaatcg ccacactcac gatgacagga ataacggcaa gtatgcgccg
      541 gatgatgtat cccagcatcg ggccagacct ctatgggcaa cgagtcatag aaggcggtgc
      601 gcagtttccg ccgtgaagcg ccgcgtcggg gacgcgacgc ttacttgtcg caaaggtcag
      661 tcggccttct caatattcca gaagacagga accggagaag gaatcatgcc ggtcagcttc
      721 ttgctccggg cttgtggcgc cgcatactga cccagcggga tatagatcac attctccagg
      781 acgtgcttct ggatgcggac ggcgacctcg cgctgctttt ccggtgtgtc cgcttcgatg
      841 aactcggcac ggagggcctc gagctcatcg tccttcggcc aaccgaacca accctggtca
      901 ccgcggccgt tcagcatcgg gctgatgagc ggtgagttga tttcgggcac catccagttg
      961 gtgaagaaca ggttccatcc gccttcagag ggcttcgcct ggctcgcgcg acgcgtgacc
     1021 agcgtctgcc agtccatggg ctgcatgtcg acgttgaagc cgacttcacg cagagcctga
     1081 gcggcgacga ctggctgctt ttgcaaagaa ataacgtccg tcggctgcat caggacaacc
     1141 ggcgtgccat cgtagccggc ttccttgaga agcttcttcg cgccttcctt gtcgccgccc
     1201 ttgatcagcg tttcggcgcc gctctcatcc cccagcggcg ttccgcatcc gaaaatggcg
     1261 ccgcagattt tgtagtattt cggatcgcca atcatggtgc cgagcacatc cgcctggttc
     1321 agcgccttca gtgcagcctg ccggattttc acattgtcga agggtgggtg cttgaagttc
     1381 atgcgcccca tcgtctggta gccgaggtcg tcgcgcgttt cgacgacgac atcgtcgttg
     1441 ccttcgagca gcggcagtag atcgatctgc acctgttcga tatagtcgat ctcaccggac
     1501 agaagcgcgt tgatggtcgt ctgggcgtcg ggcatggtca cccacttgac cttatcaacc
     1561 ttgacgacct ttccgccagc catccagctc ggtggctctt cacgcggcac gtagtcggca
     1621 aatttctcgt aggtgacgct gacaccgggc tgatattcgg actccacgaa aacaaacggt
     1681 cccgatccaa tatattcggt aattgcttgg tcaggcggcg tattcgccac gcgctccggc
     1741 atgatgaacg gtggaaccgc tgactgtttt ccgacgattt cgaggagcgc ggggaatggc
     1801 ttcttgagtt tccaaacgat cgtcttgtcg tcggccgcct cgaggctttc cgtgacgtcg
     1861 aaaatgagct ggccgcccga atcccgcttt ccccagcgct tgagcgaggc cacagcatcc
     1921 ttggccgtca cgggattgcc gtcgtggaat ttcaatccat cccgcaattt gaaggtgtag
     1981 acgagcttgt catcggagat tgtccagtcc gccatttgcg gccggggttg gaaattttcg
     2041 tcgacggcca ccagcacgtc ataaatcatg taggcgtgat cgcgggtaat gtgcgccgtc
     2101 gtgatgatcg ggtcgagcac acgcaacccc gaatgcatca cgccagtgat gactttttcc
     2161 gccgcggccg gctgaagcag gctggtgcct gcgagcattg tggcggcgag caggccggtc
     2221 acagtccgcg actgcactgg tgtgcggcct agggccattc gactcttaca atggagcttc
     2281 cacatcgcgc ttcccctgtt ggttggagat ccttccgagc tcatgatcag tgcctcctca
     2341 acgtcgttca gaacgtcgag cgtggcaatc ggattatcat taactggctg gtgagcgtgc
     2401 cgagcggcgg aactctggcc ccccgggcga caagcggatg ccctcagcca gcttctgcca
     2461 tgggagcgta gctggatcga gcggcatcgg cccgggcgcc gtgcagacca aaatggcctc
     2521 ggcaataggc tcgaaatcag cccggaaatg caccgagctc ttgttcacca ggatagcctg
     2581 ttcgcgcggc tcaatgccga cgaaacgata catggcctga tcggccattt gcgctttgtg
     2641 cgttgttacg acgatccgca ccccgtcgat ggcgagacat gccgacggcc cgaggttgag
     2701 atgcgtgccg ccgtagtagg gcccggtcgc gtggagctgt ccattcgaga gcttttcaac
     2761 ccggaaggag gcttcgagcg gactgtctcc cggaacgcgt gacttgccac cgagtgcgat
     2821 cgaaatctcg gcgccttcac cggcctgatg agcagcgcgc gccgcttccg cgtcagcgat
     2881 gaggccaatt gcggcgcgtt tggcgccgtt gcggaggagg gcgcggagca ggcccatggt
     2941 gtcggaatcg ccaccagcgc cgggattatc ttgcgtatcg gcgataacga caggccgggt
     3001 ggccttctcg gccagtttca tggcgatctg gacgccttca tccggatcat aggcctgtcc
     3061 cttgaaagct aattcgttgg cgagcacgag ctccatcatt tcgttagcgg tcttatcggc
     3121 ggctgcttgc gtctcgccat aggcgagcag gctcggcgcg cactcctcga aatccgacgc
     3181 tgggaagccc atcagaaatg aggtgctgaa aacctcgccg gcctccagtt ccgcgacgcg
     3241 ctgatagatg ctgcgcgccg gctccagtga tgtgcactgc caggggatgg ggatgaggaa
     3301 tggcagacgt cggaaagcct tcgcaaaggg gcgccccctg gccatca
//
LOCUS       DASUMG010000414         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_936864, whole genome shotgun sequence.
ACCESSION   DASUMG010000414 DASUMG010000000
VERSION     DASUMG010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936864"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VFX46_06860"
     CDS             complement(<1..572)
                     /locus_tag="VFX46_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX5600336.1"
                     /translation="MKPKTFVITALAAVVSSIAAVVVYTTHNQWDQGRIAGEKLVPAL
                     ASGEADVRSLVISHEGETITLESNADGQWTLKERDGYPADPDKVRSLLVKLAQAELIE
                     AKTRMPDRHALLELEDPSAEGAKSRSLKILDGNGNVVADLIIGKRHWEAFGTGKSGTY
                     VRKAGDAQTWLANVEFNLYARIRSWIEPKI"
     gene            complement(604..2604)
                     /locus_tag="VFX46_06865"
     CDS             complement(604..2604)
                     /locus_tag="VFX46_06865"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX5600337.1"
                     /translation="MATKRTSSSERHAGWLSRLPRHTLAWTGLVLAGIVFLSINLISA
                     TALRSVKADFTKEGLYTISEGTRRALSQIEEPIDVRVYYSHKLGEAAPSFAKNFERVR
                     TLLEQYRSISGNKLRVSYIDPEPFSDAEDRAVAAGLRGIRLNPEGDLGYFGMVGANST
                     DDEETIGFFAPERERFIEYDVTKLIYALANPKKPVIGLLSTLPLEGGLNPMMGMRGPP
                     LPPQMVMQQIREVFEVKSLSKELDEIPEDVDVLMVVQPDGLSAKTAYAIDQFALKGGR
                     ILAFVDPVPETLQPPSPMSMGATPNFDEFGKLLKAWGIEFDAKQVAGDIEHARRVQFG
                     GGVNNVVTDYVAWLSLNSSNFDQGDVLSAGIERLNLASTGFLNAAEGATTKLAPVVQT
                     SSQATSIPTEKVSPMPDAVALLREYKPGGKPLVIAARVSGEAKSAYPDGPPREEKAED
                     KKSEDNESGNAADAGAGSSDNASESKEASAEHRTEGNINVLLVADTDLLSDQFWVDVR
                     EFLGQQIAIPNAHNSAFVVGALENLSGSDALLTLRGRGIVDRPFELVAEIRRDAERRY
                     REKEQALTAKLKELQDQLAQLERVGEGETIILTDKEREAIERFRSEMLAVRRDLREVK
                     RALREDIDRLDAWLKFANIGAVPLLLGVGATCWAVLRRRRRA"
     gene            complement(2621..>3273)
                     /locus_tag="VFX46_06870"
     CDS             complement(2621..>3273)
                     /locus_tag="VFX46_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600338.1"
                     /translation="VALTGAFAFYVGNFFDRNQADLTSFFVYHPWLFLLLVPAVAMRL
                     WAEERKTGTIELLMTLPISPSDAILGKFLAAWAVIGVALVLTFPMWITVNVLGTPDNG
                     VILTSYVGSFLTAGAFLAIGSCMSALAKNQVIAFIVAAAVCFLLLMSGVDMVLNAFRV
                     WAPQFVVAAIQSLSFLSHFEDFSKGIVSLPALIFYASVIVFALFANRIIIDQKKAA"
BASE COUNT          634 a         1016 c          987 g          636 t
ORIGIN      
        1 aagattttcg gctcaatcca agagcggatg cgcgcgtaaa ggttgaactc gacgttggcg
       61 agccaagtct gcgcatcccc agccttacgg acgtacgtgc cgctcttgcc cgtgccaaac
      121 gcctcccagt gccgcttgcc aatgatcaga tcagcaacga cgtttccgtt gccgtccaaa
      181 atcttgagcg agcgggactt ggccccttcg gccgacgggt cctccagctc gaggagggcg
      241 tggcggtccg gcatccgcgt tttcgcttcg atcagctcgg cctgggccag cttcacgagc
      301 aaggagcgta ccttgtcggg atcggctggg tagccatccc gttccttgag cgtccactgg
      361 ccgtcggcat tgctttcgag cgtgatcgtt tcaccctcgt ggctgatgac gaggctgcgg
      421 acgtcggcct cacccgacgc cagtgccggg accagcttct cgcccgcgat gcgaccctga
      481 tcccattgat tgtgcgtcgt gtagacgacc accgcggcga tgctggatac gacagcggcc
      541 agtgcagtta tcacgaacgt cttgggtttc atggtcgtga tctccgagca atgactggtg
      601 tcgtcaggcc ctgcgccggc gacgcaggac tgcccagcat gtagcaccga cgccgagcag
      661 cagcggcacc gcgccgatgt tggcgaactt caaccaggca tcaagacggt cgatgtcctc
      721 gcgcagcgca cgcttcactt cacgcagatc gcggcgaacg gccagcattt cgctgcggaa
      781 ccgttcgatg gcttcgcgct ccttgtcggt caggatgatc gtttcgcctt cgccaacgcg
      841 ctcgagctga gcgagctgat cctgcagctc cttgagcttt gcggtcagcg cctgctcctt
      901 ctcgcggtag cggcgctccg cgtcgcggcg gatttcggcc acgagctcga acggccggtc
      961 gacgatgcca cggccgcgta gcgtgagcag ggcatcggat ccggagaggt tctccaatgc
     1021 tcccaccaca aacgccgagt tgtgcgcgtt cggtatggcg atctgctggc cgaggaactc
     1081 gcggacatca acccagaact ggtcgctcag gagatcggtg tcagcgacga ggaggacgtt
     1141 gatgttcccc tctgtccgat gctctgctga ggcctcttta gattccgagg cgttgtccga
     1201 cgatcccgcg cctgcatcag cagcatttcc ggactcattg tcttcggact tcttatcttc
     1261 agccttttct tctctcggcg gaccgtctgg ataggcgctc tttgcctcgc cagaaactcg
     1321 cgccgcaatt accagcggtt tgcctccggg cttgtactcc cgcagcagag caactgcatc
     1381 cggcatcggt gaaaccttct cagtcggaat gctagttgcc tgcgagctgg tctgcacaac
     1441 cggcgccagc ttcgtggtgg ccccttcggc tgcgttcaga aagcctgttg aagcgaggtt
     1501 cagccgctcg atcccggcgg agagaacgtc gccttggtcg aaattgctgc tgtttaggga
     1561 gagccaagcc acatagtcgg tgacgacgtt gttcactccg ccaccaaact gcacacgccg
     1621 cgcatgctcg atgtcgccgg cgacttgctt agcatcgaac tcgatgcccc aggctttgag
     1681 cagctttccg aactcgtcga agttcggagt ggcgcccatg gacatgggcg agggtggctg
     1741 cagcgtctcc ggcacgggat cgacaaaggc cagaatccgc ccgcccttca gggcgaactg
     1801 atcgatggca taagccgtct tcgccgaaag cccgtcaggc tggacgacca tcagcacatc
     1861 gacgtcctcg ggtatttcgt ccagctcctt gctgaggctc ttgacctcga atacctcgcg
     1921 gatttgctgc atcaccatct gcggcggcaa aggcggcccg cgcatgccca tcatcggatt
     1981 gaggccgcct tcgagcggca gtgtagagag gaggccgatc accggcttct tcgggttggc
     2041 cagcgcatag atgagcttgg taacgtcgta ctcgatgaac cgctcacgct cgggcgcaaa
     2101 gaagccgatc gtctcctcgt cgtccgtgga gttggcccca accatgccga agtagccgag
     2161 gtcgccttcc ggattcaggc ggatgccccg cagtcccgca gccacggcac ggtcctcagc
     2221 atccgagaac ggctcgggat cgatataaga aactcggagc ttgttgcccg agatcgagcg
     2281 atactgctca agaagggtcc tcacccgctc gaagttcttg gcgaaggaag gcgccgcctc
     2341 gccgagcttg tgtgagtagt agacccggac gtcgatcggc tcctcgatct ggctcagcgc
     2401 cctccgggtg ccttcggaaa tcgtatagag gccctctttc gtgaagtcgg cctttaccga
     2461 tcgcaatgcc gtcgcgctga tcagattgat cgacaggaaa acgatccccg cgaggacaag
     2521 gcccgtccag gccagcgtat gccgcggcag gcgggaaagc cacccggcgt gcctttcaga
     2581 agatgatgta cgctttgtag ccattgcgca cgtcctccgc tcaagcggct ttcttctgat
     2641 cgatgatgat ccggttcgcg aacagagcga acacaatcac cgatgcgtag aagatcaaag
     2701 ctggcagact gacgatccct ttcgagaaat cttcgaaatg ggacaggaag ctgaggctct
     2761 gaatggctgc aacgacgaac tgaggcgccc agacgcggaa ggcattgagc accatatcca
     2821 cgccgctcat cagcagcagg aagcagaccg cggccgcaac gatgaaggcg atgacctgat
     2881 tcttggccag cgccgacatg cacgagccga tggccaggaa tgcgcctgcg gtcaggaagc
     2941 tgccaacgta gctcgtgaga atgacgccat tatccggggt ccccagaaca ttgactgtga
     3001 tccacatcgg gaaggtcagc acgagggcca cgccaatgac tgcccaagcg gccaggaact
     3061 tgccgaggat ggcatcgctt ggcgagatcg gcagcgtcat cagaagctcg atggtgcccg
     3121 tcttccgttc ctccgcccag agccgcatgg caacggccgg cacaagcagc aggaacagcc
     3181 acgggtgata gacgaagaag ctcgtgaggt cggcctgatt acggtcgaag aagttgccga
     3241 cgtagaaggc aaacgcgccg gtgagcgcga caa
//
LOCUS       DASUMG010000415         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_615478, whole genome shotgun sequence.
ACCESSION   DASUMG010000415 DASUMG010000000
VERSION     DASUMG010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615478"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VFX46_06875"
     CDS             complement(<1..889)
                     /locus_tag="VFX46_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5600339.1"
                     /translation="MSSIQLPEPSPEILARRDTIVADLKALVGEEAVISDEDGRRAYE
                     TDGLTAYRKMPLAVVLPQSTEEVSAVLRYCHQYGIKVVARGAGTSLCGGALPAEDAVV
                     VGVSKMNRVLEVDYANRIAVVQTGITNLAVTNAVSKDGFFYAPDPSSQLACTIAGNLA
                     MNSGGAHCLKYGVTTNNVLGVKMVLMDGEIVEIGGAHLDSPGYDLLGLIIGSEGQLGI
                     VTEATVRILRSAENARPMLLGFESSEAAGACVAAIIGSGIIPVAIEYMDKPAIEVCEA
                     FAKAGYPLDVEALLIVEVEG"
     gene            1152..1538
                     /locus_tag="VFX46_06880"
     CDS             1152..1538
                     /locus_tag="VFX46_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX5600340.1"
                     /translation="MHSRGLIGLVFLVAALGPAAAQDAENGEQVFRKCRACHQIGEGA
                     RNTVGPQLNNIVGRTAGSVEGYNYSKANKKAADDGLVWTEENLMAYLEDPRKFMPGTK
                     MVFPGLRDEKERKDVIAFLKKFSQQQ"
     gene            1667..2443
                     /locus_tag="VFX46_06885"
     CDS             1667..2443
                     /locus_tag="VFX46_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX5600341.1"
                     /translation="MNGLLPGKPRVGLFVTCLVDLMRPSVGFAAVKLLEQAGCSVEVP
                     VTQTCCGQPAYNTGDRKDAAALARQTISAFEGFDYVVAPSGSCAGMLKLHYPALLSDD
                     PEWAGKAKAFAERVYELISFLVDVRGVETVDAEFQGRVTYHDSCSGLRELGIKDQPRR
                     LLASVKGLELAEMTDSEVCCGFGGTFAVKYPDISNAMVEKKTANVAATDPDLLLAGDL
                     GCLLNIAGKLKREGRQIAVRHVAEVLAGEISDPPIAGLGK"
     gene            2529..>2917
                     /locus_tag="VFX46_06890"
     CDS             2529..>2917
                     /locus_tag="VFX46_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX5600342.1"
                     /translation="MKPTTKSFKENVRVALTDAQLQRALAGLPTGLVANRAAARARLP
                     EFEDLREICRDIKDHTLAHLDLYLEEYERNATAAGAKVHWAATAAEAREVILKICREV
                     NAKIVTKGKSMITEEIGLNAELQAAGI"
BASE COUNT          595 a          883 c          898 g          541 t
ORIGIN      
        1 agccttcgac ctcgacgatc agcaacgcct cgacgtcgag cgggtacccg gccttggcaa
       61 aagcctcgca cacctcgatc gccggcttgt ccatgtactc gatcgcgact ggaatgatgc
      121 ccgagccgat gatggcggcg acacaagcac ccgccgcctc gctcgactcg aagccgagca
      181 gcatcggccg ggcattctca gccgaacgca gaatgcggac cgtcgcctct gtgacgatgc
      241 cgagctgccc ttccgagccg attatcaagc ccaggaggtc gtatccgggg gaatcgagat
      301 gagcgccgcc gatttcgacg atctcgccat ccatcagcac catcttgacc ccaaggacgt
      361 tgttcgtggt cacgccgtac ttgaggcaat gtgcgccgcc tgagttcatg gcgagattgc
      421 cagcaattgt gcaggcaagc tggctcgaag gatcgggcgc atagaagaag ccgtccttgc
      481 tgaccgcatt cgtaacggcg aggtttgtga tgccggtttg cacaacggca atacggttgg
      541 cgtaatcgac ctccagcacc cgattcatct tcgacacgcc gaccactacg gcatcctcag
      601 ctggcagagc accgccgcag agcgaggtcc ctgccccgcg cgcgacaacc ttgatgccat
      661 actggtgaca atagcgaagg actgcgctga cctcctcggt cgactgcggc agcacgacgg
      721 cgagcggcat tttccggtag gccgtcagcc catccgtctc ataggcacgc cggccatctt
      781 catcgctgat cacggcctcc tccccgacaa gagccttcag gtcagcgacg attgtgtctc
      841 gtcgggcgag aatttccgga ctaggctccg gcagctgaat ggacgacatt cgagcctcag
      901 gtcattgtcg caacgaattt gccaccacgg cttctaattg gcacggctgc ggtggaaggg
      961 gcaacctggt cgcgttgcga tcaccgttta tgtgggtcat gactcggttg ttgagcagag
     1021 cggcaagttc gtccgctggg cgcagcgccg ccgtgcttca tcaagagctg agccaacccg
     1081 actcgttacc gcaggtccag gtaggtagcg gtaccaatcg gctcggaatg actgatagag
     1141 gaaacggaac gatgcactca aggggattga taggattggt cttcctcgtg gctgcactag
     1201 gcccggccgc tgcacaggac gctgagaacg gcgagcaggt gttcaggaaa tgccgtgcct
     1261 gccatcaaat cggagagggc gccaggaata cggtcggtcc gcagctcaac aacatagtcg
     1321 gaagaaccgc agggtcggtt gagggctaca attactcaaa ggccaacaag aaggcggctg
     1381 atgacggcct tgtctggacc gaggaaaacc tgatggccta tctcgaggac ccgagaaagt
     1441 tcatgccggg aaccaagatg gttttcccag gactccgcga tgagaaggaa cggaaggacg
     1501 tcattgcatt cctcaaaaag ttttcgcagc agcaataggc gcacggccaa gctcgccaac
     1561 ggcctcttgg cgcgatggcg gggcgcggaa taaaagcagc ggagtttccg gcgcggatct
     1621 cgtccgcgcc gcaaataaat tcaaatgatc ggaggcattt cggcctatga acggactcct
     1681 gcccggcaag ccacgcgtgg gcctttttgt cacctgcctc gtcgatctga tgcggccctc
     1741 cgttggcttt gctgcggtca agttgctgga gcaggctggc tgctccgtcg aggtgccggt
     1801 cacgcaaacg tgttgtggtc agcctgcgta caacacgggt gacaggaagg atgcggcggc
     1861 gttggcgcgc cagacgatct cggcgttcga gggctttgat tacgtcgtcg ctccttccgg
     1921 ctcctgcgcc ggtatgttga agctgcatta tcccgccctg ctatccgacg atcccgaatg
     1981 ggcgggaaag gcgaaggcct tcgcggagcg tgtctacgag ctgatttctt tcctcgtgga
     2041 tgtgcgcggc gtcgagaccg tcgatgccga attccagggc cgcgtcacct atcacgacag
     2101 ctgctccggg ctccgcgagc ttgggatcaa ggaccagccg cgccggcttc tcgcctctgt
     2161 gaaaggacta gagctggccg aaatgacgga ttccgaggtt tgttgcgggt ttggcggcac
     2221 gttcgctgtg aagtatccgg acatctccaa cgcaatggtc gaaaagaaga ccgccaacgt
     2281 ggcagcgacg gaccctgatc tgttgcttgc tggcgatctc gggtgcctgc tgaatatcgc
     2341 cgggaagctc aagcgcgagg gccgccagat cgcggttcgc cacgtggcag aggtgcttgc
     2401 cggtgaaatc tccgacccgc cgatcgccgg tttaggaaag tgagcaaccc cttggccttg
     2461 cctacccgat cccagtcgcg ccaagctgat tacccccgca cgccctacgg cggcacgagg
     2521 attgtctgat gaagcctacg acgaagtctt tcaaagagaa tgtccgcgtc gcgctgaccg
     2581 acgcccaact ccagcgggcg ctggctgggc tgccgaccgg cctcgtcgca aaccgtgccg
     2641 ctgcgcgggc gcgcttgccg gagttcgaag atctccgtga gatctgccgg gacatcaaag
     2701 accacaccct cgcgcacctc gacctctatc tcgaagagta cgagcgcaat gccaccgccg
     2761 cgggagcgaa ggtgcactgg gcggcaacag ccgcggaggc gcgcgaggtc atcctcaaga
     2821 tttgccggga ggtgaacgca aagatcgtca ccaaaggcaa atccatgatc accgaagaga
     2881 tcgggctcaa cgccgagctg caggccgctg gcattga
//
LOCUS       DASUMG010000416         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1097268, whole genome shotgun sequence.
ACCESSION   DASUMG010000416 DASUMG010000000
VERSION     DASUMG010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097268"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="VFX46_06895"
     CDS             <1..1207
                     /locus_tag="VFX46_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX5600343.1"
                     /translation="AYLVEAGRAVTAAMVTRGEKPAVLGALMKYQSTERMRQAVNDAM
                     DVHGGRGICDGPTNYLQSAYQMVPIAITVEGANILTRTLITFAQGALRSHPYLYRELQ
                     AVQNPDPKRGLELFEPIFLDHVSFSVANAFGALFHNLTGGLFGAAPAKAFGVEGWYRQ
                     LWRASHNFAVVADLSVALLGGGLKTKQKITGRLADALSELYLLACALKRFEDDGRPEV
                     DQPIVDLVARNGLYRFQNALAGAIDNFPVAWARVLMRLLVFPLGRRFRPAPDRLAREA
                     VKLAIQPGTVRDRLTRYIYVTRDPNDPTGLLEVALEKAVAAEETEKKLERAIRAGQVR
                     RFHGIDWIGDAVRQHVISETEAQLLREVEALREKVIAVDHFDPAEVKPHYMSTSHSIQ
                     AANGKAAAE"
     gene            1225..>2732
                     /locus_tag="VFX46_06900"
     CDS             1225..>2732
                     /locus_tag="VFX46_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX5600344.1"
                     /translation="MAQDMTQLKDWRFSVDREGIAWAIFDREGESANALGRRPLEELG
                     AIVEHVESRARTGEIKGLVFMSGKAKNFIVGADIREFDALDTESKVIEAIRPVNAMLD
                     RIERMPVPVVCAIHGACVGGGLELALACHYRIATRDDSTRVGFPEVKLGLFPGFNGTA
                     RSIRQVGPLTAMQNMLTGSLLRAPVARALGYIDELVNNRHQLRWAARKAVLQRRKSKP
                     AGITKAILAKWPFRGLLAKKLRTETAKKVREDHYPAPFRLIDLFETHGGNLEALKHAE
                     TRAFAPLMVSPTARNLCRVFRLMEMLKDQAPKDLGWKPLRVHVIGAGTMGADIAGWCV
                     ASGMEVSLQDISPEQIKKGIEAQEKLFARKFKTKAQRDAAKARLIADPQGRNISRADV
                     VIEAVVEKLEVKQEVFKSIEHKLKPGAVLATNTSSIMIEDIARPLSDPGRLIGLHFFN
                     PVSRMPLVEVVRGEASREEELKKGAAFVTAIDKLPLIVKSVPGFLVNRVLAP"
BASE COUNT          521 a          841 c          890 g          480 t
ORIGIN      
        1 cgcctacctc gtcgaggccg gacgcgcggt aacagcggca atggtcactc gtggcgagaa
       61 gccggcagtg ctcggcgcgc tgatgaagta tcagtcgacg gagcggatgc gccaggccgt
      121 caacgacgcc atggatgtgc acggtggccg cggcatctgc gatgggccaa cgaactatct
      181 tcagtcggcc tatcaaatgg tgccgatcgc aatcaccgtc gaaggcgcca acattctcac
      241 gcggacgctc atcaccttcg cccagggcgc gctgcggtcg catccgtatc tctatcgcga
      301 gctgcaggcg gtccagaacc ccgatcccaa acgggggctt gagctttttg agcccatttt
      361 tctcgaccac gtgtcgttca gcgtcgccaa tgcgttcggg gctttgtttc acaacctcac
      421 tggcgggctg tttggtgctg cgccagcgaa agcattcggc gtcgagggct ggtatcggca
      481 gctctggcgg gcgagccaca acttcgctgt tgtggccgat ttgagcgtgg cgctgctagg
      541 cggcggcctg aagacgaagc agaagatcac gggccgtctc gctgacgccc tttcggagct
      601 ttatctcctc gcctgcgccc tgaagcgctt cgaggacgat ggccggcccg aggtggacca
      661 accgatcgtc gatcttgtcg cgcgaaacgg tctttaccgg ttccagaatg cgcttgcggg
      721 tgccatcgac aatttccccg tcgcctgggc gcgcgttctc atgcggttgc tcgtgtttcc
      781 gctcgggcgc cgtttccggc cggcacctga tcgtctcgcg agggaggcgg tgaagctcgc
      841 gatccagccg gggaccgttc gcgaccgtct gacgcgatac atctacgtta cgcgtgaccc
      901 gaacgatcca accggcttgc tcgaggtggc cctggaaaaa gccgtcgctg cagaagagac
      961 ggaaaagaaa ctggaacgcg ccattcgggc aggtcaggtg cgccgattcc acggcatcga
     1021 ctggatcgga gatgccgtgc ggcagcacgt tatcagcgag acggaggccc aacttctgcg
     1081 cgaggtcgag gcgctgcggg aaaaggtcat cgccgtcgat cactttgacc ccgccgaggt
     1141 caaaccccat tacatgagca ccagtcacag cattcaggcc gcaaacggca aagccgcggc
     1201 ggaataagcc gcaaggaggc aggcgtggcg caggatatga cgcaactgaa ggactggcgc
     1261 ttttccgtcg accgggaagg catcgcctgg gccatattcg atcgcgaggg tgaaagcgcc
     1321 aacgcgctgg gccggcggcc gctcgaggaa ttgggcgcca ttgtcgagca cgtggagagc
     1381 cgcgcgcgga caggcgagat caaggggctc gtcttcatgt ccggcaaggc gaagaacttc
     1441 atcgtcggcg ccgacattcg cgagttcgat gcgctcgaca ccgaaagcaa ggtgatcgag
     1501 gccatccgcc ccgtgaacgc catgctcgac cggattgagc gtatgccggt gccggtcgtc
     1561 tgcgccattc acggcgcctg cgtcggcggg ggattggagc tggcgttagc gtgccattac
     1621 cggatcgcca cgcgcgatga ctcaacgcgt gtgggctttc ccgaagtgaa gttgggcctg
     1681 ttccctggct tcaacggcac cgcgcgctcg atccgccagg tcggtccgct caccgccatg
     1741 cagaacatgc tgacgggctc gctgcttcgc gcacccgtcg cccgcgccct cggatacatc
     1801 gacgagctgg tgaacaatcg ccatcagctt cgctgggcgg cgcgcaaggc ggtgctacaa
     1861 cgccggaaat cgaagcctgc aggcatcacc aaggctatcc tcgccaaatg gcccttccgc
     1921 ggactgcttg cgaaaaagct tcgcaccgag acagccaaga aggtacgcga ggaccattat
     1981 cccgcgccat tccgcctgat cgatctattc gagacgcacg gcggcaacct cgaagcgctg
     2041 aaacacgccg agacccgcgc cttcgcgccg ctgatggtca gcccgaccgc gcgcaatttg
     2101 tgccgcgtgt tccggctgat ggagatgctg aaagatcagg cgccgaagga tctcggctgg
     2161 aagccgctgc gcgtgcatgt gatcggcgcg ggcaccatgg gcgcggatat tgcgggctgg
     2221 tgtgtggcct ccggcatgga agtctcgctg caggacattt cgccggagca gatcaagaag
     2281 ggcattgaag cccaggagaa gctgtttgcc cgcaagttca agacgaaagc gcaacgcgat
     2341 gccgccaagg cgcgcctcat cgcagaccca caggggcgca acatcagccg ggcggacgtg
     2401 gtgatcgagg ctgtcgtcga aaagctcgag gtcaagcagg aggtcttcaa atccattgag
     2461 cacaagctca agccgggtgc tgtgctcgcg acgaacacgt cctcgatcat gatcgaggat
     2521 attgcacgac cgctgagcga tccgggccgg ctgatcggcc tgcacttctt caatccggtg
     2581 tcgcgcatgc cgctggtcga agtggtgcgc ggcgaggcga gccgcgagga agagctgaag
     2641 aagggcgcgg cgttcgtcac ggccatcgac aagctcccgc tgatcgtgaa gagcgtaccg
     2701 ggtttcctcg tgaaccgcgt gctggcgccg ta
//
LOCUS       DASUMG010000417         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_134899, whole genome shotgun sequence.
ACCESSION   DASUMG010000417 DASUMG010000000
VERSION     DASUMG010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134899"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1612)
                     /locus_tag="VFX46_06905"
     CDS             complement(83..1612)
                     /locus_tag="VFX46_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600345.1"
                     /translation="MATLLQQQHYRAYQPRPLTKSEIKDVEILYGGLHWRAEHVMKAV
                     FENLGYKARPLPTATREDLLLGRELADIGQCCPTSFTTGNLANFLRTEAKRIGEQAVA
                     DKYVYVTAGSCGACRFGQYHQSYELALRNLGLESFRLFLIDQNRMDQGALKGGGLEIS
                     IPLTLGTVWAILCTDALQSLEYRTRPYEVEPGSTDRVVRESVDYLYEVFRKRPDKGKK
                     WGPLVWHLTTDYFTKALREIYRKFEAIEVDRLRVKPVVKITGEFYLQTVEGDPNYNIH
                     RWLESEGAEVYPAPIVVWLDYWIRFYVQELEDRMGTDRTAQAKIWALKSLQKLFRATY
                     NRMRKALGNIPHEIPDQYELRRLAAPYFHHRLSGGEGDMLIGKALWAHLHKKAHMICE
                     LSPYSCMPNTMSVGAMAGVLGQHPDLLYAPIEVKGDAEVHALSRCQMILTEAKKRAVR
                     EYVKVMEKTGLDPEAVRKVLDANPEMRKATYRVPHKGVAGMAANTMLHVADRFYARQA
                     A"
     gene            complement(1793..>2887)
                     /gene="rnd"
                     /locus_tag="VFX46_06910"
     CDS             complement(1793..>2887)
                     /gene="rnd"
                     /locus_tag="VFX46_06910"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX5600346.1"
                     /translation="TEFLREQTYWPVLCLIQLAGPEEEVIIDPLAPGLDLKYFYDLMA
                     DERVVKVFHAGRQDIEIIYAKAKLVPHPIFDTQVAAMVCGFGESVSYVNLVKKLTNED
                     VDKTSRFTDWSRRPLTQKQLAYALADVTHLRQVYLQLKAELERTGRAHWLDEEMATLT
                     DPATYEMKPEDAWQRLKMRAKNRRSFAVLIELAAWRERLAQTLDVPRSRILRDEALYD
                     IANQAPTDTAQLGELRTLSEGFSRSARAREIIEAVKRGLARNPKTLPQIPTGQALPAE
                     ATALVDLLRVLLKAAAARNRVAAKLIADTSDLERIAVAEDPDVPALRGWRRELFGEDA
                     LRLKRGEVALTVAKGEVVTVPVSRVRQPGN"
BASE COUNT          524 a          901 c          873 g          589 t
ORIGIN      
        1 tggtaatcgc gccaaatcac ttggccatct tcgacaacca ccgctttgat ggttgtcgag
       61 ccgacgtcca tgcccaccgt tttcatgcgg cttgcctcgc gtagaagcga tcggcaacgt
      121 ggagcatcgt gttggcggcc ataccggcaa cgcccttatg gggcacgcgg tacgtggcct
      181 tgcgcatttc cggattggcg tcgagcactt tgcgcacggc ttccgggtcg agcccggtct
      241 tctccatcac cttcacgtac tcgcggacgg cgcgcttctt ggcctcggtg aggatcatct
      301 ggcagcggga gagtgcgtgc acctcggcat cgcctttgac ctcgatgggc gcgtagagca
      361 gatccggatg ctggccaagc acgcccgcca tggcaccgac gctcatggtg ttcggcatgc
      421 aggaataggg cgacagctcg cagatcatat gcgccttctt gtgcaggtgc gcccacagcg
      481 ccttgccgat cagcatgtcg ccctcgccgc cggagaggcg gtggtggaaa tagggtgccg
      541 cgagccgccg cagctcgtat tggtccggta tctcgtgggg aatattgccc agggccttgc
      601 gcatcctgtt gtaggttgcg cggaatagct tctgcagact cttcagcgcc cagattttcg
      661 cttgcgcggt ccgatcggtg cccatccggt cctccagctc ctgcacatag aagcggatcc
      721 aatagtcgag ccacacgaca atgggggccg gataaacctc cgcgccttcg gattcgagcc
      781 agcggtggat gttgtagttg ggatcgccct ccaccgtctg cagataaaac tcgccggtga
      841 tcttcactac gggcttcacc cgcagccgat cgacctcgat ggcctcgaac ttacggtaaa
      901 tctcccggag cgctttggtg aaatagtcgg tcgtcaaatg ccagacgagc ggaccccact
      961 tcttaccttt gtcgggccgc ttgcggaaaa cctcataaag gtagtccacg ctctcgcgaa
     1021 cgacgcgatc ggtgctgcct ggctcgacct cataaggccg cgttctgtac tccaggctct
     1081 gcagagcatc cgtgcacaga atggcccaga ccgtgcccag cgtcagcggg atgctaatct
     1141 caagaccgcc gcccttgagt gcgccctggt ccatcctgtt ctgatcgatc agaaacagcc
     1201 ggaacgactc gaggcccagg ttccgcagcg ccagctcata gctttggtga tactggccga
     1261 agcggcacgc cccacatgag ccagcggtga cgtacacata cttgtcggcc acggcctgct
     1321 cgccaatccg cttcgcctcc gtgcgcagga agttcgcgag attgccggtg gtgaagctcg
     1381 tggggcagca ttgtccaatg tcggccagct cccgaccgag caaaagatcc tctcgcgttg
     1441 cggttggcag tgggcgcgcc ttgtagccga ggttctcgaa cacggccttc atgacgtgct
     1501 cggcgcgcca atgaagcccc ccatagagga tctcgacgtc tttgatttct gatttcgtga
     1561 ggggacgcgg ctggtaagcg cgatagtgtt gctgctgcag cagagtggcc atgcgctacg
     1621 ctccgtccgg agatcccgac gagaccagca accctcagca ggccgattgg ttcccgaaat
     1681 cgagcctcag atcatggcgt ttgagcacaa catattccag ggccagatgg caagtctggt
     1741 aggcgagcac cgtgcacgga gctcgccgtg cgggcatgct taatgagatg agctaattgc
     1801 caggttgccg tacgcgagaa acgggaaccg tgacgacctc ccctttcgca acggttaatg
     1861 ccacctcacc acgcttcaat ctgagagcgt cctctccgaa gagctcgcgc cgccatccgc
     1921 gcagagctgg gacgtccggg tcctctgcca ccgcgatgcg ctcgagatca cttgtatctg
     1981 caatgagctt tgctgcgacc cggttgcgag ccgccgctgc cttgagcaga acacggagga
     2041 gatcaaccag cgccgttgct tctgccggta gggcttgacc ggtggggatc tgtggcagcg
     2101 tcttcggatt gcgcgcaagg ccgcgcttga cggcctcgat gatctcccgt gcccgcgctg
     2161 accgcgagaa gccttcgctc aacgtgcgta gctcgccaag ctgcgctgtg tcggtgggcg
     2221 cctggttggc gatatcgtaa agcgcctcgt cccgcaggat gcgcgagcgc ggcacatcca
     2281 gcgtttgggc cagcctctcc cgccaggcag ccaattcgat gagcactgcg aacgaccggc
     2341 ggttcttggc ccgcatcttg agacgctgcc aggcatcttc cggcttcatc tcataggttg
     2401 cggggtccgt gagagtggcc atctcctcgt caagccaatg ggcacgaccc gttctctcga
     2461 gctcggcctt gagttgcaaa taaacctggc gcagatgcgt tacgtcagcc aaagcgtagg
     2521 cgagctgctt ttgggtcagg ggccgccggc tccagtccgt aaagcgggaa gtcttgtcga
     2581 cgtcctcgtt ggtgagcttc ttcacgaggt tcacgtagct caccgactcc ccgaagccgc
     2641 agaccatggc cgcgacctga gtgtcgaaaa tcggatgtgg gacgagcttc gccttggcgt
     2701 agatgatctc gatgtcctga cggccggcgt ggaaaacttt caccacgcgc tcgtccgcca
     2761 tcaagtcata gaagtacttc aggtcgagcc ccggcgcgag gggatcgatg atgacctctt
     2821 cctccggccc tgccagctgg atgaggcaca atacgggcca gtaggtctgc tcgcgcagga
     2881 actcggt
//
LOCUS       DASUMG010000418         2222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_937507, whole genome shotgun sequence.
ACCESSION   DASUMG010000418 DASUMG010000000
VERSION     DASUMG010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937507"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..2114
                     /locus_tag="VFX46_06915"
     CDS             141..2114
                     /locus_tag="VFX46_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEX5600347.1"
                     /translation="MLKRVLIANRGEIALRIIRTARRLGIETVAVASEPDMGLPYTRA
                     ADIVVPIGPGPASESYLVFDKIIGAAKETGADAIHPGYGFLAENPDFAEAVAAAGLVF
                     IGPSAESMRLLGGKSEAKAVAMKAGVPVVPGYQGEDQRPEILAEAARGIGYPVLIKAV
                     AGGGGRGMRLVEREEDFAPLLASAQREAQAAFGDPRVLIERVITSPRHIEVQVFGDSH
                     GNAVHLFERDCSLQRRNQKVIEEAPAPGMSQQLRAKMTEAAVKLVKAVGYCGAGTVEF
                     LVEGGELGPDAPWYFIEMNTRLQVEHPVTEAITGLDLVEWQLRVAAGEPLPLKQNQIA
                     YSGHAIEARLCAEDATRDYLPSIGRIVAFETPELEGLRVDAGVETGSVVSPFYDSMIA
                     KLIAFAPDRAGAIERLMSALEQTVVAGVKTNAAFLHGLLSHSAFRAGEIDTGFIGREL
                     GRLTSKGTDAAAVAYGVVSLIRQQQKEMEAKRRRISNEPYSPWAALDAFQLGEPRRHS
                     LDVVVEGQKTSVEVLWNGNSPSVLLPGEPGPIQCNAINAKAIEDGQRVFVLHNLRQTE
                     VAWPVYDVGAADESDGTGNVRAPINGRVVKVFVEEGTLLDKGQRIAVIEAMKMEHVLV
                     APIRGKIAKLTASEGQQVTEGTVLATLVEEKAE"
     gene            2114..>2222
                     /locus_tag="VFX46_06920"
     CDS             2114..>2222
                     /locus_tag="VFX46_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T4"
                     /protein_id="HEX5600348.1"
                     /translation="MVPGPRNLITDIPGLRVGNAHDERVLSGVTAVILDK"
BASE COUNT          430 a          628 c          758 g          406 t
ORIGIN      
        1 ccgcagcgga aggctcaccc tgccgtgcac tcgcgtcatc tcggcttgct gggtgagccg
       61 atcatcgccc agtactacgg gttttgcgtg ccgaaataat tgggctagtg agggccccaa
      121 gggcgggggc tgatacggtc atgctgaaac gggttctcat tgccaatcgc ggcgagatcg
      181 cgctgcggat catccgcaca gcgcgtcggc tcggaatcga gacggtcgcg gtggcatccg
      241 agcccgatat gggcctgcct tatacgcgcg cagccgatat tgtcgtgccc attggccctg
      301 ggccggcgag cgaaagctac ctggtgttcg acaagatcat cggtgcggcg aaggaaacgg
      361 gcgccgatgc catccatcct ggctacggct tcttggccga aaatccagac tttgctgaag
      421 ccgtagctgc ggcagggcta gtattcatcg gcccgagcgc ggaatcgatg cgcctgctcg
      481 gcggcaaatc ggaggcgaaa gccgtcgcca tgaaagccgg cgtgccggtg gtgccaggct
      541 atcagggcga ggaccagcgg cccgaaattc tcgccgaggc ggcgcgcggg atcggctatc
      601 cggtgttgat caaagcggtg gcagggggcg gcggccgcgg catgcggctc gtcgagcggg
      661 aggaagactt tgcaccgctg ctcgccagtg cccaacggga ggcgcaagcc gcattcggcg
      721 atccccgtgt gttgatcgaa agggtgatca cctcaccgcg gcatatcgag gtgcaggtgt
      781 ttggcgacag ccacggtaac gccgtccacc tgttcgagcg cgactgctca ctccagcggc
      841 gcaaccagaa ggtcatcgag gaagcgccgg cccccggcat gtcgcagcaa ctcagggcga
      901 agatgaccga agcggccgtc aagctggtca aggcggtcgg ctactgcggt gctggcacag
      961 tcgagtttct ggtcgagggc ggcgagcttg ggccagacgc cccctggtac ttcatcgaga
     1021 tgaacacgcg gctgcaggtc gagcacccgg tgaccgaagc catcaccggg ctcgatttgg
     1081 tcgaatggca gttgcgcgtt gcggccgggg agccgttgcc attgaagcag aatcagatcg
     1141 cctattctgg acacgccatt gaggctcgcc tgtgcgccga ggatgcgacg cgtgactacc
     1201 taccttcgat cgggcggatc gttgcattcg aaacgccgga attggagggg ctgcgggtcg
     1261 atgcgggagt ggaaacaggc tctgtcgtgt cgccgttcta tgactcgatg atcgccaaac
     1321 tgattgcgtt cgctccagat cgggccggag ctatcgagcg attgatgagc gccctggagc
     1381 agacggttgt ggccggcgtg aagacgaacg ctgcattcct gcacggattg ctttcgcatt
     1441 ccgcatttcg tgcgggggag atcgataccg gcttcatcgg ccgggagctt ggccgactga
     1501 cgtcaaaggg caccgatgcc gcggccgtcg cttatggcgt cgtctcgctc atcaggcagc
     1561 aacagaaaga gatggaggcc aagcgtaggc gcatttcgaa cgagccttat tcaccttggg
     1621 cagctctcga tgctttccag ctgggcgagc cacgccgcca ttcgctcgac gtagtggtgg
     1681 agggccaaaa aacgtctgtc gaggtcttgt ggaacggaaa cagcccgagc gtcctgctgc
     1741 cgggtgagcc tggtccaatc cagtgcaatg cgatcaacgc caaggccatc gaggatgggc
     1801 agcgcgtgtt cgtgctccat aacctacggc aaacagaggt tgcctggccg gtctacgacg
     1861 ttggtgctgc ggacgaaagc gacggcactg ggaatgttcg ggcaccgatc aacggccggg
     1921 tggtgaaagt tttcgtggaa gaaggcacgc ttctcgacaa agggcagcgg atcgcggtta
     1981 tcgaggcgat gaagatggag cacgttctcg tcgcccccat tcgtgggaaa atcgccaagc
     2041 tgaccgcaag cgaaggtcag caggtgaccg aagggacggt gctggcgacg ttagtcgagg
     2101 agaaggcaga gtagtggtac ccggcccccg caatttgatc acggacattc ctggtttgag
     2161 agtcgggaac gcgcatgacg agcgcgtgct gagcggtgtt actgctgtca tcctcgacaa
     2221 gc
//
LOCUS       DASUMG010000419         2732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_776737, whole genome shotgun sequence.
ACCESSION   DASUMG010000419 DASUMG010000000
VERSION     DASUMG010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776737"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /gene="nadC"
                     /locus_tag="VFX46_06925"
     CDS             <1..465
                     /gene="nadC"
                     /locus_tag="VFX46_06925"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX5600349.1"
                     /translation="VDTRKTTPGLRALEKYAVRAGGGMNHRIGLFDAVLIKDNHIAVA
                     GGIGPAIAAARARVGHMVKIEVEVDSLEQLAEALNHPIDAVLLDNMTPAQLAEAVRLV
                     GGRVITEASGGIDLSTVRAVAESGVDLISVGALTHSAPVLDIGLDIEIGTTT"
     gene            662..874
                     /locus_tag="VFX46_06930"
     CDS             662..874
                     /locus_tag="VFX46_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600350.1"
                     /translation="MVEFVVLWGVTAIAAMLIGGLLAGLKKRDYSSWMAWSFIFPPAV
                     VLLLILPSLPQRPRRRTLDEEDAAEF"
     gene            complement(907..1296)
                     /locus_tag="VFX46_06935"
     CDS             complement(907..1296)
                     /locus_tag="VFX46_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5600351.1"
                     /translation="MKLIEHQAQECEDWRPGVTTRMLVSAVSGSVQLCIFDQYCQPGH
                     GAPTHLHAVEEVLTVLEGRAEIWVGDDRTEVGAGHSVIVPAGVKHGFRNVSETDPLHV
                     RATLAAPIFEASYEDRNELAIRYVPSG"
     gene            complement(1383..>2732)
                     /locus_tag="VFX46_06940"
     CDS             complement(1383..>2732)
                     /locus_tag="VFX46_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX5600352.1"
                     /translation="IHGMHAAAGILTARGGMTSHAAVVARGMGRPCVCGAGQLRIDAK
                     AGEMTAPNGKVYRAGDIITIDGSTGEVLEGRVRMRQPELSGDFATLMSWADAVRRLGV
                     RANADTVRDAQQALHFGAEGIGLCRTEHMFFEADRILAMREMICAEDEEGRRRALAKI
                     LPMQRADFEAMFEIMAGLPVTIRLLDPPLHEFLPHEEKEQISVAEALGVPVSRVKSRV
                     SELQEFNPMLGFRGCRLAIRYPEITEMQARAIFEAAVNAAKKTGKPVVPEVMIPLVAY
                     RKEFDILRDVIVATAEAVEKETGVKLEYQVGTMVELPRSALRAEEIAAGEGGAEFFSF
                     GTNDLTQTCLGLSRDDAAPILENYLGRSIFESDPFVSIDQDGVGELVRIGTERGRKAR
                     PDLKIGICGEHGGDPASVAFCHEIGLDYVSCSPFRVPVARLAAAQAAISGRITPKEG"
BASE COUNT          502 a          890 c          835 g          505 t
ORIGIN      
        1 gtcgacaccc gcaagacgac tccaggattg cgcgcgctgg agaaatacgc cgtgcgcgct
       61 ggcgggggaa tgaaccaccg gatcggcctg ttcgatgccg tcctcatcaa agacaaccac
      121 atcgcggttg ccggcggcat cgggccggcg atcgcggcag cccgcgcgcg ggtcgggcac
      181 atggtcaaga tcgaggtgga agtggacagc cttgagcagc tcgcagaagc tctcaatcac
      241 cccatcgacg cggtgctgct cgacaacatg acgcctgccc agctcgcgga ggctgtccgc
      301 cttgttggcg ggcgggtcat caccgaagcc tccggcggaa tcgatctcag cacagtccgg
      361 gccgttgccg aatccggcgt cgacctcatt tccgtcggtg ccctgacgca ctcggcacca
      421 gtgctggata tcggacttga catcgagatc ggcaccacaa cctagcttga ccgcccgcga
      481 cgtggttccg ggttcatcca gcaccaccgt ttggccatga tcggtagaca ggtgatacgg
      541 tgggttgagt agtgcagctc tgcaaaagct tcggctagtt tcgatttcgt tcaggatcag
      601 cgcggtttag tttagtctag tttcgggtgt gcgagaccgg ctccaagcgg gagagaccca
      661 aatggttgag ttcgtggtcc tctggggcgt gactgccatc gcggcgatgt tgatcggcgg
      721 tctgcttgcg ggcctgaaaa agcgcgatta ctcgtcctgg atggcgtggt cgttcatttt
      781 cccacccgcc gtcgtgttgc tcctcatact cccttctctt cctcagcggc cccgtcggcg
      841 aaccttggat gaggaggacg cagcggagtt ttaggaagca ccgggtagtg gccatcgcaa
      901 ggccgctcat ccagagggca catatcggat cgccagctca ttgcgatcct cgtagcttgc
      961 ttcaaaaatc ggcgcggcga gagttgcccg gacgtggagc gggtcagttt ccgacacatt
     1021 ccggaaaccg tgcttgacgc ctgccgggac gataaccgaa tgccccgctc cgacctcagt
     1081 gcgatcgtcc cctacccaga tttcggcgcg accttcgagg accgtcagaa cctcctccac
     1141 cgcgtgcagg tgggtcggcg caccgtggcc aggctggcaa tattggtcga agatgcacag
     1201 ctgcaccgac ccggacaccg ctgaaacgag catccgggtc gtaactcccg gccgccagtc
     1261 ctcgcactcc tgcgcctgat gctcgatgag cttcacgttc cgccctcatt ctggccacca
     1321 gtggcgagga tggcagccag cctgaatgca agttaccgag gcggctgagg caacaaagtg
     1381 gctcagccct ctttcggcgt gatccgcccc gaaattgccg cctgcgccgc cgcaagtctc
     1441 gccaccggca cacggaacgg ggagcaggag acgtagtcga gcccgatctc gtggcagaat
     1501 gcgaccgatg ccggatcgcc accgtgctca ccgcaaatgc cgatcttgag atccggccga
     1561 gcctttctgc cgcgctccgt gccgatacgc accagctcgc ccacgccatc ctggtcgatg
     1621 gagacgaacg gatcgctctc gaaaatgctc cggccgagat agttctcgag gatcggcgca
     1681 gcgtcgtcgc gggaaagacc gaggcaggtc tgcgtgaggt cgttggtgcc gaatgagaag
     1741 aactcggctc cgccctcgcc tgctgcaatc tcctctgcgc gaagagccga acggggaagc
     1801 tcgaccatgg tgccgacctg atactcgagc ttgacacccg tctccttttc aaccgcctcg
     1861 gctgtcgcca caatcacatc gcggagaatg tcgaactcct tccgatatgc gacgaggggg
     1921 atcatcactt cgggcacgac cggcttgcct gtcttcttgg cagcattgac cgccgcctcg
     1981 aaaatcgccc gcgcctgcat ttccgtgatc tcgggatagc ggatcgccag ccggcagcct
     2041 cggaagccga gcatcgggtt gaactcttgc agctcgctaa cacgcgattt cacgcgagaa
     2101 acaggcacgc cgagtgcttc ggcaacggag atctgctctt tctcctcgtg cggcaggaac
     2161 tcgtgtagcg gaggatcaag gagccggatg gtgaccggca gcccagccat gatctcgaac
     2221 atggcctcga agtccgcccg ctgcatgggc aggatcttgg ccaatgcccg gcgacggcct
     2281 tcctcatcct cagcacagat catctcgcgc atggcgagga tgcggtcggc ctcgaagaac
     2341 atatgctcgg tacggcagag cccgatgcct tcagccccga agtgcagcgc ctgctgggcg
     2401 tcccgcactg tgtctgcatt ggcgcgaacg ccgagccggc gcacagcgtc agcccaggac
     2461 atcagcgttg cgaagtcgcc ggacagctcc ggctgccgca tgcgcactcg gccctcgagc
     2521 acctcgccgg tcgagccatc gatggtgatg atgtctcctg cgcgatagac cttgccgttg
     2581 ggcgcagtca tctcgcccgc cttggcgtca atcctgagct gccctgcacc gcacacgcag
     2641 ggccgaccca tgccacgagc gacgacggcg gcatgactcg tcataccgcc gcgggcggtg
     2701 aggatgcccg ccgcagcgtg catgccgtga at
//
LOCUS       DASUMG010000420         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_375765, whole genome shotgun sequence.
ACCESSION   DASUMG010000420 DASUMG010000000
VERSION     DASUMG010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375765"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /gene="nuoK"
                     /locus_tag="VFX46_06945"
     CDS             complement(<1..191)
                     /gene="nuoK"
                     /locus_tag="VFX46_06945"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX5600353.1"
                     /translation="MEFSAVGLGHYLTVAAILFTLGIFGIFLNRKNVIIMLMSIELML
                     LSVNINLVAFSYFLADLVG"
     gene            complement(228..869)
                     /locus_tag="VFX46_06950"
     CDS             complement(228..869)
                     /locus_tag="VFX46_06950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX5600354.1"
                     /translation="MGLSAFFFYVFAILSVLAGAMVIIARNPVHAVLFLILAFFNAAG
                     LFILLGAEFLAMVLVIVYVGAVAVLFLFVVMMLDVDFAELRAGFIRNAPLAALIGGIV
                     LVELVMVVGFAYIQPDVSGVAAAPFPTVGSGLTNTEALGRLVYTRYVFFFQGAGLILL
                     VAMIGAIVLTLRHKPGVKRQSIAAQTARTPETAIEVVKVEPGQAIVPSGEAQG"
     gene            complement(1066..1557)
                     /gene="nuoI"
                     /locus_tag="VFX46_06955"
     CDS             complement(1066..1557)
                     /gene="nuoI"
                     /locus_tag="VFX46_06955"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX5600355.1"
                     /translation="MASIAQAIRAAFLTEFVSAFFLSMRYFFAPKATVNYPYEKGPLS
                     PRFRGEHALRRYPNGEERCIACKLCEAICPAYAITIEAGPRRNDGTRRTTRYDIDMVK
                     CIYCGLCQEACPVDAIVEGPNFEFATETREELLYDKQRLLDNGDRWERALAKNIALDA
                     KYR"
     gene            complement(1576..2640)
                     /gene="nuoH"
                     /locus_tag="VFX46_06960"
     CDS             complement(1576..2640)
                     /gene="nuoH"
                     /locus_tag="VFX46_06960"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX5600356.1"
                     /translation="MTALEIWWDYGWPLAIMLAQSLLLLLGLLFLIAYLLLMDRKVWA
                     AVQLRRGPNVVGPFGLLQSFADLLKFVFKEPVIPAGSERTIFLLAPLATATFALSAWA
                     VIPVSEDGLGRWVISDINVGILYIVAVSSLGVYGIIMGGWASNSKYAFMGSLRSTAQM
                     ISYEVSIGFVIITVLLLVGSLNMTDIVNAQRSGIGTAVGLPNTFLDWNWLVLFPMFVV
                     FFISALAETNRPPFDLPEAESELVAGFMVEYSSTPYLLYMLGEYVAMTTMCALTTILF
                     LGGWLPPIDVAPFTWIPGIIWFILKAVFVFFMFAMMKAMLPRYRYDQLMRLGWKVFLP
                     LSLAMVVLVAGVLHYGGLAP"
     gene            complement(2653..>3467)
                     /locus_tag="VFX46_06965"
     CDS             complement(2653..>3467)
                     /locus_tag="VFX46_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX5600357.1"
                     /translation="EIAAGRHSFMQAPDEARRPMVIVGSGAIARPDGAAVLSLAAQIA
                     LAAMRESLPEWNGFNVLHAAAARVAGLDLGFVPEEGGRDLLGMLSGDMEVVYLLGADE
                     IDMSAIGDAFVIYQGTHGDAGAHRADLILPAAAYTEKSGTYVNLEGRAQMTTRSVFPP
                     GAAKEDWAIIRALSPLVGHQLPYDDIFALRAAMYQEAPSLARIGMVKQEGREGVEALA
                     RMGGGQPGTAPLRSIVKDFYLTNPIARASAIMAELSRLKALADSENTVEAAE"
BASE COUNT          746 a         1120 c         1041 g          560 t
ORIGIN      
        1 tgtccgacga gatcggcaag gaaatacgaa aacgccacca ggttgatgtt gaccgaaagg
       61 agcatcagct cgatcgacat cagcatgatg atgacgttct tccggttcag aaaaatcccg
      121 aagatgccga gcgtgaaaag gatcgcggcg accgtgaggt agtggccgag tccgaccgct
      181 gagaattcca tggttggtgt cccgatccgc ttgccttccg tcccgtgtca gccctgagcc
      241 tcgcccgacg gcactatggc ctgccccggc tccaccttca ccacctcgat cgccgtttcc
      301 ggcgtgcgcg ccgtctgggc cgcgatcgac tgccgtttga cacccggctt gtggcgcagt
      361 gtcagaacga tggcgccgat cattgccacg aggaggatca gccctgcccc ctgaaagaag
      421 aagacgtaac gcgtgtagac gagacggccg agcgcctcag tgttcgtaag tccggaaccg
      481 actgtaggga acggagcggc agcaacacct gacacgtcgg gctgaatgta ggcgaacccg
      541 accaccatca cgagctcgac cagcacgatg ccgccaatca gcgccgccaa aggtgcattc
      601 cggatgaacc cggcccggag ctcggcgaag tcgacgtcaa gcatcatgac gacgaacagg
      661 aacagcaccg cgaccgcgcc gacgtagacg atgaccagca ccatcgccag gaactcggca
      721 ccaagcagga tgaagaggcc cgccgcgttg aagaacgcga ggatcaggaa gagcacggcg
      781 tgcaccgggt tccgcgcgat gatgaccatc gcgcccgcaa ggacgctcag aatcgcgaaa
      841 acgtagaaga agaacgccga caggcccatt gtgctttcgt cccgatcccc ttcagctgct
      901 cagccgggag ccgctgacgt tccatccttc tgcgccgcaa caagaatgac cccggcccgg
      961 ctccgcaggg gcacttagcc caatcggcgc cacaaaccaa ggccgaaaag gctgatctcc
     1021 atgaccggct tgcatgttcc tgccccctaa agcgcccgtt gcctctcagc gatatttggc
     1081 atccagcgcg atgttcttgg ccagagcccg ctcccagcga tcgccgttgt cgagcaagcg
     1141 ttgcttgtcg tagagcagct cctcgcgtgt ctcggtggcg aactcgaagt tcggcccttc
     1201 cacgatcgca tctaccgggc aagcctcctg acacagcccg cagtagatgc acttcaccat
     1261 atcgatgtcg tagcgcgtcg tgcgccgcgt gccgtcattg cggcggggcc ccgcctcgat
     1321 ggtgatggcg taggccgggc agatcgcttc acacagcttg caggcaatgc agcgctcctc
     1381 gccgttggga taacggcgca aagcgtgctc gccgcggaag cgggggctga gcggtccctt
     1441 ctcgtagggg tagttgaccg tcgccttcgg cgcgaagaag tagcgcatcg agaggaagaa
     1501 ggccgagacg aactcggtca gaaaagctgc gcgaatggcc tgagcaatgg atgccatgat
     1561 gagatatctc cgccgttacg gcgccagtcc gccgtaatga aggacgccgg ccaccagcac
     1621 aaccatcgcc agagacagcg gcaggaacac tttccagccg agccgcatga gctgatcgta
     1681 gcggtagcgc ggcagcatcg ccttcatcat cgcgaacatg aagaacacga aaaccgcttt
     1741 aaggatgaac cagatgatcc ccggtatcca cgtgaacggc gccacgtcga tgggtggcag
     1801 ccagccaccg aggaacagga tcgtcgtcag cgcgcacatg gtcgtcatcg ccacgtactc
     1861 gccgagcatg tagaggaggt acggcgtcga ggagtactcg accatgaaac cggccacgag
     1921 ctccgattcg gcttcgggca ggtcgaatgg cgggcggttc gtctccgcca gcgctgagat
     1981 gaagaagacg acgaacatcg ggaacaggac cagccagttc caatcgagga acgtgttcgg
     2041 caggcccacc gccgttccaa ttccgctgcg ctgcgcattg acgatgtcgg tcatgttgag
     2101 cgagccgacc agcagcagca cagtgatgat cacgaacccg atcgagacct cgtaggagat
     2161 catctgtgcg gtcgagcgca gcgagcccat gaaggcgtac ttcgagttcg acgcccaccc
     2221 gcccatgatg atgccgtaga ctccgagtga ggagaccgcg acgatgtaga gaattcccac
     2281 gttgatatct gagattaccc agcgcccgag cccatcctcg gagacaggga tgacagccca
     2341 cgccgagagc gcaaaggtcg ccgtcgcaag tggcgccagc aggaagatcg tccgctccga
     2401 gccggcggga atcacgggct ccttgaagac gaacttcagc aggtctgcaa aggattgcag
     2461 tagaccgaat ggcccaacga cgttcgggcc acgccgcagc tgcaccgcgg cccacacctt
     2521 acggtccatc agcagaagat atgcgatgag gaacaacagg ccgagcagca gcaacaagct
     2581 ctgcgccagc atgatcgcta gcggccagcc gtagtcccac cagatctcca atgcagtcat
     2641 gttgctgccg tcctattccg cagcctcgac cgtgttctcc gagtcggcga gcgccttcaa
     2701 ccggctgagc tccgccatga tcgcagaggc gcgggcaatg gggttggtca gatagaagtc
     2761 cttcacgatc gacctgagcg gcgcagtgcc cggttgtccc ccgcccatgc gagcaagggc
     2821 ctcgacgcct tccctgccct cctgcttcac catgccgata cgtgccagcg atggagcttc
     2881 ctgatacatc gctgcgcgca aagcaaaaat atcgtcatag ggcaattggt ggccgaccag
     2941 aggtgacagc gcgcgaatga tcgcccagtc ctccttcgcc gcacctggcg ggaagacgga
     3001 ccgagtggtc atctgagcgc ggccctcgag gttgacgtag gtgccgctct tttccgtgta
     3061 ggctgcagcc ggcaaaatga ggtccgcccg gtgggcgccc gcatcgccgt gggtgccttg
     3121 ataaatgacg aacgcatctc cgatcgcgct catgtcgatc tcgtcagcgc cgagcaggta
     3181 aacgacctcc atgtcgccgc tcagcatgcc caacagatca cggccgccct cctccggcac
     3241 gaagccgaga tcgagaccgg cgactcgcgc tgccgccgca tgcagcacat tgaagccgtt
     3301 ccactccggc aaggattccc gcatcgccgc gagggcaatc tgtgccgcca gggacagcac
     3361 cgcagcgccg tccggtcgag cgatagcccc gctgccgaca atcaccatcg gccgccgggc
     3421 ttcgtccgga gcctgcatga acgagtgccg gcccgcagcg atctccg
//
LOCUS       DASUMG010000421         3492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_536441, whole genome shotgun sequence.
ACCESSION   DASUMG010000421 DASUMG010000000
VERSION     DASUMG010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536441"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VFX46_06970"
     CDS             complement(<1..508)
                     /locus_tag="VFX46_06970"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEX5600358.1"
                     /translation="MLRAQLGEDVYTSWFHSMEFEHFDGKTVRVTVPVKFLKNWIQSH
                     YSEALLRCCAAEFEGVERVEVALRQPSPVRTVSQDAPGMSDCRAAAVPTDSAPPVSGW
                     QAPHLVAPAIVTRTHVGDFEGSPLDPRYTFESFAVGPSNRMAHAGANQVAETVLSDDR
                     GFNPLFIHS"
     gene            complement(764..1207)
                     /locus_tag="VFX46_06975"
     CDS             complement(764..1207)
                     /locus_tag="VFX46_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX5600359.1"
                     /translation="MGTGQAVHIVDVDVREVWKRLKEEPTAVLIDVRTQAEWAYVGLP
                     DLSSIGKRPFLIEWQTFPDNRVNPEFVERLSEMLEVAGIDKQAELFFLCRSGGRSKRA
                     AQAMAEAGYTACRNVADGFEGPLDAERHRGRTAGWKAAGLPWAQG"
     gene            complement(1860..2126)
                     /gene="rpsT"
                     /locus_tag="VFX46_06980"
     CDS             complement(1860..2126)
                     /gene="rpsT"
                     /locus_tag="VFX46_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX5600360.1"
                     /translation="MPNTTSAKKAVRKTARRTAVNKARRSRMRTMVRKVEEAIASGQK
                     EAALQALAVAEPILARTAQKGVIHKNTAARKVSRLVARIRAMPD"
     gene            2444..3457
                     /locus_tag="VFX46_06985"
     CDS             2444..3457
                     /locus_tag="VFX46_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEX5600361.1"
                     /translation="MRILVTGAAGFIGYHTAEALLARGDTVVGLDNLSAYYDVRLKEA
                     RLARLQRHPQFQFARVDLADREGVAQVFAEHRPERVVHLAAQAGVRYSLKNPHAYVDS
                     NVVGTLHVLEECRAHKVDHLVLASTSSVYGANTRMPFSVEDNVDHPLSLYAATKKSCE
                     LMAHSYAYLYRLPVTVLRFFTVYGPWGRPDMALFKFTRAILAGEPIEVYNNGQHARDF
                     TYIDDIVEGVVRTLDRPATPDPDWSGETPNPATSSAPYRLYNIGNNRPVELMDLISCL
                     ERALGRSATKTFLPLQPGDVPRTAANIDHLANHVGFRPNTPIEVGVERFVAWYREYYG
                     VEA"
BASE COUNT          695 a         1086 c         1001 g          710 t
ORIGIN      
        1 cggaatggat gaagagcggg ttgaagccgc gatcgtcgct caaaacggtt tccgcgacct
       61 gattggcgcc cgcgtgcgcc atccggttcg acgggccgac agcaaagctc tcgaaggtgt
      121 aacgcggatc aagaggcgag ccctcgaagt cgcccacgtg cgtgcgcgtc acaatcgctg
      181 gcgcaaccaa atgaggtgct tgccagcctg acacgggcgg cgccgaatcg gtgggaacgg
      241 cggccgcgcg gcagtcggac atcccgggcg catcttggga cacggttcgc actggactcg
      301 gctgacgaag agccacttcg actctctcga caccctcgaa ctcggcggcg cagcaccgaa
      361 gcagtgcctc cgaatagtgg gactgaatcc agttcttcaa gaacttcacc gggacggtca
      421 cacgtaccgt cttcccgtcg aagtgctcga attccatcga gtggaaccaa ctcgtataga
      481 catcctcgcc cagttgggca cgaagcattt tgcgtacctt gtgcccccgg ggctcctgag
      541 cacctggggc ggccgcctgt ccccctgagc gcgtcagagc cggcccatcg gacctagcct
      601 gagcatcgct gctcccgctg cccgcacttc gcgcggcagg tctcattggt tccacgctat
      661 tcatggctac acccgctgct tctgttgacc ctctgtcacg gcgtcgtgcg agtgaccgcc
      721 gccacggtcg atccgccgac gcttcttcag atttcaatgg cgcttatccc tgcgcccagg
      781 gcagaccggc ggctttccaa ccagccgtcc ggccgcggtg gcgttcggca tcgagtggcc
      841 cctcgaaacc gtccgcaaca ttacgacagg ccgtatagcc tgcctcggcc attgcttgcg
      901 cagccctctt gctacgcccg cctgaccggc aaaggaaaaa aagctctgcc tgcttatcga
      961 ttccggccac ctccagcatt tccgagaggc gttctacgaa ctcgggattg acccggttgt
     1021 ccggaaaagt ttgccactcg atgaggaatg gccgtttgcc aatggaggag aggtccggca
     1081 acccgacgta cgcccactcc gcctgggtgc gcacgtcgat gaggaccgcc gtcggctcct
     1141 ccttcagccg tttccacacc tctcggacgt cgacatccac aatgtggacg gcctgcccag
     1201 tacccacctc acaaacctcc actgatgaac gcaacactcg tcacaccgtt tattcagtgt
     1261 tcgactgaag aaacggacga ggacgcaacg tatgaccaag cttttgcccg gaattacgca
     1321 ggcagacaga agaattacct attgttttca aatacgctgg agcaccatca attgctctct
     1381 actagtacaa tgagtacgca agacttgaat gacccgataa acgacatttt tggccaccgt
     1441 ctgtcgaccg aatggatttc gtattaacca cacgtctcga tgtgagacaa gatcgctcgg
     1501 cggctccgag ctcctgacga aagctcggca ccagctatcc acaccgcttt ggaaaagtgg
     1561 gtctgaaacg ataggattcg tggagaactc ccttgagtcc acaacaagat tcgaaggcgg
     1621 gaagggccga tgccaaagaa aaaggcgccc gcgtggcgag cgccccaaaa agctcatttt
     1681 atcttgaaac ccgaccttcc gatttagtgg gcaacctgtc gatgcctttc ggcaaacctg
     1741 tcaggaattc ttgattcctg agcggttttt ggccgcaccg ccgcgactcg ctgggctgtt
     1801 gagcagatct tgacgttgca aatttatcac attgatttgc gaaggtaatg atgctcgctt
     1861 tagtcgggca tagcgcggat acgcgctacg agccgcgaca ccttgcgagc ggccgtgttc
     1921 ttgtgaatca cgcctttctg agccgttcga gcgagaatcg gctcagcaac cgcaagtgcc
     1981 tgcagagctg cttccttctg gccggaagcg atggcttcct cgaccttacg caccatcgtt
     2041 ctcatgcgcg agcggcgtgc cttgttgact gcggtgcggc gggccgtctt gcgcacggcc
     2101 ttcttggccg acgtcgtatt tggcatgtgt tgttcgtcct ctttagtgct tgatgtcagg
     2161 gccggcgaat cggtcatcgg ccgcatcttg ggccggacct gacagcggaa tacatcaaac
     2221 cgcccgggac cgggggttcc gggcggcacg ttgcagatcg ctataatgcc gctgcttatg
     2281 acggtcaacg gcccacagaa gtggtacgtg agccgcttct gccaactcgt caaggcgagc
     2341 ggtaccacct atagtagtcg cggaggcggc cagccacacg agcatcgcca ggcaggatcg
     2401 ccgatccgac gcacgggaca ggatcaacga acaggcagct gtgatgcgca tattggtgac
     2461 tggtgcggct ggcttcatcg gatatcacac ggccgaggca ctcctggcgc gtggtgacac
     2521 tgtcgtcggt ctcgacaacc taagcgcgta ttacgatgtc cgactcaagg aggcgcggct
     2581 cgcccgactt cagaggcatc cgcagttcca atttgcccgc gtagatctcg ccgaccgcga
     2641 gggcgtagcc caggtgtttg ccgagcatcg ccctgagcgg gtcgttcacc tcgcggcaca
     2701 ggcaggcgtt cgatattccc tcaagaatcc gcacgcctac gtcgactcga acgtcgtcgg
     2761 cacgctacac gtgctcgagg aatgccgcgc acacaaagtc gaccatctgg tgcttgcttc
     2821 cacgagctcg gtttacggcg ccaacacgcg catgccgttc tcggtcgagg acaacgtgga
     2881 ccatccgttg agcctctatg cggcgaccaa gaagtcgtgc gagctgatgg cgcacagcta
     2941 cgcctatctt tatcggctgc cggtgacagt cttgcgcttc ttcaccgttt atggcccatg
     3001 ggggcggccc gatatggcgc tcttcaagtt cacgcgggcg atcctcgccg gggagccgat
     3061 cgaggtctac aacaatggcc agcatgcgcg ggatttcacg tatatcgatg acattgtcga
     3121 aggcgttgta cgcacgctcg atcgcccagc gacacccgac ccggattgga gcggtgagac
     3181 gcccaacccg gcaacttcct ctgcgccgta tcgcctctac aacatcggta acaatcggcc
     3241 ggtggagctc atggatctga tttcctgcct cgagcgagcg ctcggccgct cagcaacgaa
     3301 aactttcctg ccgttgcagc ctggtgatgt tccgcgcacg gctgctaaca tcgaccatct
     3361 cgctaatcac gtaggctttc ggcctaatac gccgatcgag gtcggggtcg agcgatttgt
     3421 ggcctggtac cgcgagtatt atggggtcga agcttgaggt cgcggaccag gacgctggca
     3481 ctcgaataga ac
//
LOCUS       DASUMG010000422         3103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_135670, whole genome shotgun sequence.
ACCESSION   DASUMG010000422 DASUMG010000000
VERSION     DASUMG010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135670"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VFX46_06990"
     CDS             <1..937
                     /locus_tag="VFX46_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5600362.1"
                     /translation="AETGLKLLSRLRSGEIQLDGQSLTVSGQAPDTAVAALIRQQLRD
                     LPKGYAGAETIEIRSDAMIWAEEEAKRQAEAEARRQAEEAQRKAEEEARQAEQAAEAP
                     QEAASETAEAKAGSTTETRIANVSPLPSDEGGAAASSETSEEPADEPSAEQRAALGED
                     GSHAEGGQTPEGQTAAAVQQAALAASRDVDERCQQALAKFAPHGTINFDRAKTDLKHA
                     SVITLSRMAEVAASCPNLPIRIEGHADAEGSPEGNQVLSEMRAEAAVKFLVDKGVSLD
                     RIKSVGYGATRPVAPNDTPASRALNRRVEIFIGDH"
     gene            1110..2855
                     /locus_tag="VFX46_06995"
     CDS             1110..2855
                     /locus_tag="VFX46_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600363.1"
                     /translation="MMTLLWQTAALLLAAYFLGAFLGCLIRRTFFGKTPVTDTEPAYA
                     GMPSSVAPGAMAEPEPVSTAPAYQATTQPAYPAESAEPLAAAPQVDETTAAEPEVGGV
                     VHYPRAETPKNDEASAPVSEAPSHPRAEAEPVSPPPQESSAPYRPVIPPVGDRKRSIG
                     TSAAAAAALAAATARYRAAQEAARAAGTIPAAAGSFDGAAAAVTAPPASTPATDNPDD
                     LTRIRGIDPELSQKLSTLGVRKFSDIAAWRKEDIQRISAALGFRGRIEQENWIEQAQI
                     LATGTETAYSRRRANMAPLAKPSADEGERRAPPQAQPASAEVAPAPPAAATEPPAKKP
                     PPTGLGRHHLQRIKGITPDIERLLNLHGVHRYDHIARWTAADVKKFDQLLGRKGRIAQ
                     ENWIEQAQILANRGLTAFARQFDEQHTAAPPRLRPAHLLDAIREAQAANNAGQAKPAE
                     EGEGGPAKPKFDLSTLRSVRSEAFQSDKAEVHPPQSPSPGVPKQVDDLKRIRGIGVAL
                     EKRLNGLGYWTYEQIANWTAVDVDRISRALDIPGRIEQENWIEQAQILATGGQTWFAR
                     RRDRGEVAIDPSSNE"
BASE COUNT          621 a          953 c         1008 g          521 t
ORIGIN      
        1 cgccgagaca ggtctcaagc ttctctcacg actgcggtcg ggtgaaattc agcttgatgg
       61 ccagagcctc acggtctcag gacaagcgcc cgataccgcg gtggcagcac tcatccgcca
      121 gcaacttcgc gacttgccca aaggttatgc gggcgcggag accatcgaaa ttcgctccga
      181 cgcgatgatc tgggccgagg aggaggcgaa gcggcaggcg gaagctgagg ctcgcaggca
      241 agctgaagag gcgcaacgca aggcggagga ggaagcgcgg caggctgagc aagctgccga
      301 ggctcctcaa gaggcggctt cggaaactgc cgaagcaaag gctggctcca ccaccgaaac
      361 tcggattgcg aatgtttcgc cgcttcccag cgatgagggt ggcgcggctg catcctcaga
      421 aacctcagaa gagccagcgg atgagccgtc tgcagagcag cgcgctgcct tgggggaaga
      481 cggttcacac gccgaagggg ggcaaacgcc ggaaggtcaa acagccgccg ccgttcagca
      541 ggcggcactt gcggcgtcgc gggacgtcga cgagcgatgt cagcaggcac tggcgaagtt
      601 cgcaccccac ggcacgatca atttcgatcg cgcaaaaacc gacctcaagc acgcgagtgt
      661 gatcacgctt tcccggatgg cagaggttgc cgcgtcttgc cccaacctcc ccatcaggat
      721 cgaaggacat gcggatgccg aaggttcgcc ggaaggcaac caggtgctgt ccgaaatgcg
      781 ggctgaggct gcagtgaagt ttctcgtcga caagggcgta tcgctcgatc gcatcaagtc
      841 cgtcggctac ggcgcaacgc gccccgtggc tcccaacgac acgccggcga gtcgtgcgct
      901 caatcggcgc gttgagatct tcattggcga ccactgattt cggggacggg ggcgcaggcc
      961 atggattatc tcgttgtgaa gctgttctgg tatctggtgc tggctttcgc catcggattg
     1021 tttgtcggct gggtttcttg ccagcgcgtc agcgatcggt agctcaaagc tcggtgctat
     1081 aggcagggtt cgcccacggg gaccgaccga tgatgacttt gctctggcaa acggcagccc
     1141 ttttgctcgc agcttacttc cttggtgctt ttctgggttg cctgatccgg cggacgttct
     1201 tcggaaagac gccagtgaca gacacggagc cggcttatgc cggaatgccg agttctgtgg
     1261 cgcccggagc tatggctgag cctgagccag tttcgactgc cccggcctac caggcgacga
     1321 cccagccggc ctatccagcc gagagcgctg aaccattggc cgctgcgccg caggtcgacg
     1381 agacgacggc agcagagcca gaggttggcg gagtagtgca ttatccgcgg gcggagacac
     1441 ccaaaaatga tgaggcctcc gcgcccgtct cggaagcacc gtcacatcca cgtgcagagg
     1501 cagagcctgt ctcgccgcct ccccaggagt ccagcgcgcc ttatcggccc gtcattcccc
     1561 ctgtgggtga tcgaaagcgg tccatcggaa cgtcagccgc cgcagctgca gcgcttgccg
     1621 ccgcgacggc gcgctatcgc gcagcgcagg aagccgcgcg tgcagccggc actattccgg
     1681 cggccgccgg gagcttcgac ggtgccgctg cggccgtaac tgcgcccccg gcctcgacac
     1741 ctgccaccga taacccggac gacctcaccc gcatccgcgg catcgatccg gagctgagcc
     1801 aaaaactcag cacgctgggt gttcggaagt tctcggacat tgccgcgtgg cggaaggagg
     1861 acattcagcg gatcagcgct gcgctcggat tccgtggacg gatcgagcag gagaactgga
     1921 tcgagcaggc ccaaattctc gccaccggga cggagacggc ctactcccgt cgccgtgcga
     1981 acatggcgcc gttggcgaag ccgagcgcag atgagggcga acgccgggct ccgcctcagg
     2041 cacagcctgc ctcagcggag gtcgctccag ctccgccggc tgctgcgaca gagcctccgg
     2101 cgaaaaagcc gccaccaaca ggtttggggc gacatcatct gcagcgcatc aagggcatca
     2161 cgccggacat cgaacggctg cttaacctgc acggcgttca tcgctacgat cacatcgccc
     2221 gttggacggc tgccgacgtc aagaagttcg accagcttct cgggaggaag ggccgcatcg
     2281 cgcaagaaaa ctggatcgag caagcgcaaa ttctggcaaa ccgcggcttg accgcgttcg
     2341 cacgtcagtt tgacgagcag catacggcag caccgccgcg cttgcgtccg gcgcaccttc
     2401 tggacgcaat tcgggaagct caggcggcga acaatgcggg acaggccaaa ccggccgagg
     2461 aaggcgaagg cggtccggcg aaacccaagt ttgatttgtc gaccctcaga tcggtgcgct
     2521 ccgaagcctt ccagagcgac aaggcggagg tgcacccgcc gcagtctccc tcgccgggtg
     2581 tgcccaaaca ggttgacgat ctcaagcgta tccggggaat cggcgtcgcg ctcgaaaagc
     2641 ggctgaacgg cctgggctat tggacttacg agcagatcgc caattggacg gctgtagatg
     2701 tcgatcgcat cagccgtgcg ctcgacatcc ccggacggat cgaacaggaa aactggatcg
     2761 agcaggcgca aattctcgcg accggtggcc agacatggtt tgcgcgccgg cgtgacaggg
     2821 gagaggtggc catcgatcca tcgtcaaatg aatagcgctg gcttgggaga ctggagtgtc
     2881 acgctagttg attgtcttct cgagtgtata tgccagctct tgcgcaacgc tttcgttcgt
     2941 gatgaacacg agtggctttg gactcggcgg gaggcgactt tgactagggg aacaacaaac
     3001 gtgctcaaac gtatggcttt gctgatgagc gcggcggcat tggccggatg cgccagcagc
     3061 aatgccgggc cttatggtga tcccggggcc cgcccattgc cgc
//
LOCUS       DASUMG010000423         2082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_777325, whole genome shotgun sequence.
ACCESSION   DASUMG010000423 DASUMG010000000
VERSION     DASUMG010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777325"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1656)
                     /locus_tag="VFX46_07000"
     CDS             complement(31..1656)
                     /locus_tag="VFX46_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003498456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5600364.1"
                     /translation="MAETPEEFDYVIVGAGSAGCVLANRLSADPSVRVLLLEAGGKDD
                     YVWIHIPVGYLYCFGNPRVDWGFETVSEPGLNGRKLQYPRGKVLGGCSAINGMIYMRG
                     QARDYDHWRQLGNPGWGWEDVLPFFLKHEDQQALEPEEFGGVHARGGEWRVEKPRVRW
                     EILDAWAEAAEQAGIPRIRDFNTGNNEGSSYFHVNQRKGFRWTTAKGFLKPALNRPNL
                     TLLTHATVESLTLEGKRVTGVAFHQKGSASRSVVARREVILAAGAIGSPMILQRSGIG
                     PGDVLKAAGIEVRHELRGVGENLQDHLQIRCAFKVSGVRTLNERSQTLWHKAGIALEY
                     ALWRSGPMSMAPSQLGCFARSDPSRETPNLQYHIQPLTLPKFGEPLDPFPAFTASVCN
                     LRPTSRGHVRITSPDPNAHPEIRPNYLSTEEDRRVAADAIRLTRRIAQQPALARYKPE
                     EFKPGPHIDGDEALAQAAGDIATTIFHPVGTAKMGTDEMAVVDPRLRVHGLQGLRVID
                     ASIMPTITSGNTAAPTMMIAEKGASMIIEDWRG"
     gene            complement(1697..>2082)
                     /locus_tag="VFX46_07005"
     CDS             complement(1697..>2082)
                     /locus_tag="VFX46_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600365.1"
                     /translation="GYLVGCLDDPSRNRLFSDISYFAEFAHLTRKYPAHLHINLTTRW
                     RSQGIGKRLVSTFADCAAEAGVPGLHVITGEGHRNNRFYVACGFVQLGSAVWNGNRIV
                     FFGRKLARSGDYSGEPGLPRRDPLE"
BASE COUNT          406 a          656 c          635 g          385 t
ORIGIN      
        1 gggcaaaacc gctcgcgaga taagcaggtc tcagccgcgc caatcctcga taatcatgct
       61 ggcacctttc tcggcgatca tcatcgttgg tgcggccgtg ttaccagagg tgatcgtcgg
      121 catgatcgaa gcatctatga cccgcaaacc ttgcagccca tgcaccctga ggcgaggatc
      181 gacaacggcc atttcgtcgg tgcccatttt ggctgtgcca acggggtgga aaatcgtggt
      241 ggcgatgtcg cccgcagcct gtgccagggc ctcgtctccg tcgatgtgcg gaccgggctt
      301 gaactcctcg ggcttgtagc gagccagcgc cggctgttgc gcgatacgcc gggtcaaccg
      361 gatggcgtcc gcggcgacgc ggcgatcctc ctcggttgac agatagttcg gcctgatctc
      421 gggatgcgcg tttggatccg gcgacgtgat acggacatgc ccacggctcg tcggccgcag
      481 gttgcagaca cttgcggtga acgcagggaa tgggtcgaga ggctcgccga acttcggcaa
      541 tgtcagcggc tgaatgtggt actgcaaatt gggcgtctcg cgactggggt cggagcgcgc
      601 gaaacaaccg agctgggagg gcgccatcga cataggcccg ctccgccaca gcgcatactc
      661 gagcgcgatt ccggccttgt gccagagcgt ctgcgatcgc tcgttgagcg tgcgcacgcc
      721 cgaaactttg aatgcacagc ggatctggag gtggtcttgc aggttctcgc cgacaccacg
      781 aagctcgtgg cgaacctcga tgccggcagc cttgagcaca tcgcccggac ctattcctga
      841 gcgctgcaga atcatcggcg agccaatggc gccggccgcg aggattacct ctcgccgcgc
      901 cacaaccgaa cgcgaggccg atcctttctg atgaaaggcc acaccagtaa cgcgcttgcc
      961 ttcgagcgta aggctctcga ccgtagcgtg agtgagcagc gtgaggttcg gccggttgag
     1021 tgcgggcttc aagaatcctt tcgccgtggt ccaccgaaaa cccttgcgct ggttcacgtg
     1081 aaaatagctc gagccctcgt tgttgccggt attgaagtcg cggatgcggg ggatgccggc
     1141 ctgctcggcg gcctctgccc atgcgtcgag aatctcccag cgcacgcgcg gcttctcaac
     1201 gcgccactcg ccgccccgag catgcacacc tccgaactcc tcgggctcga gggcttgctg
     1261 gtcctcatgc ttgaggaaaa acgggagtac atcctcccac ccccacccgg gattgccgag
     1321 ctggcgccag tgatcataat cgcgggcctg cccgcgcatg tagatcatgc cgttaattgc
     1381 ggagcagcca cccagaacct tgccgcgcgg atactgaagc ttgcggccgt tgaggcccgg
     1441 ttcacttacc gtctcgaagc cccaatcgac cctcggattt ccaaagcaat acaggtaccc
     1501 aaccgggata tggatccaga cgtagtcatc cttgccaccg gcctcgagaa gcagaacgcg
     1561 aacgctcggg tcggctgaaa gcctattggc caacacgcag cccgccgagc cggcgcccac
     1621 gatgacatag tcgaactctt ccggggtttc ggccatcgcg cttcgctcct cccaagcgtg
     1681 tccatttgaa ccgtcattac tccagcgggt cgcgtcgagg aagaccaggt tcgccggaat
     1741 aatcgccgct gcgggcgagc ttccggccga agaacacaat ccggtttccg ttccagaccg
     1801 cggatccgag ctggacaaat ccgcacgcaa cgtagaaccg gttattgcga tgtccttcgc
     1861 ccgtgatcac atgtaggcca ggaacgccgg cctccgctgc gcaatccgca aacgtcgaca
     1921 caagccgctt gccgattccc tggctgcgcc aacgggtcgt caagttgata tgcaggtgcg
     1981 ctggatactt ccgcgtcaga tgcgcgaact cggcgaagta ggagatgtcc gaaaagagac
     2041 gattgcggga gggatcatcg aggcaaccga cgagatagcc ga
//
LOCUS       DASUMG010000424         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_777326, whole genome shotgun sequence.
ACCESSION   DASUMG010000424 DASUMG010000000
VERSION     DASUMG010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777326"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VFX46_07010"
     CDS             <1..575
                     /locus_tag="VFX46_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600366.1"
                     /translation="DEVAEQIQVRGFELQPTVNLKRPVEAEDGTQAEAAFTVIRPAFG
                     DFPEARAQVLRHHTPEHIWQKRYLPQDNGLTGLLEVALVIADPSEAAERYSRFLGRTV
                     EREGASLLINLDRGGLRFLDGRGAAEHFGRVSRPPLPAVGAITLTSSDLDKTRYFFLS
                     NGLRPGAINPGHLLIDESEALGVHLIIVPE"
     gene            complement(635..1363)
                     /locus_tag="VFX46_07015"
     CDS             complement(635..1363)
                     /locus_tag="VFX46_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600367.1"
                     /translation="MAIPSGATPLLAGCGLHTYYGSSYVLRGVSLAISAGEAVGLMGR
                     NGMGKTTLIRTLMGLVAPRVGQVLIDGQDATGMPTFRRAQAGLAYVPEERGIFASLSV
                     AENLAIAERAGIDGSSKWTRERVLDLFPRLSQRLSHRGGQLSGGEQQMLAIARALLAN
                     PRLLILDEATEGLAPIIREEIWSTIAKIRNAGVATLIVDKTVDAITSLADRIVILVKG
                     EVVFEGSPASLKADPALMHRHLGV"
     gene            complement(1363..2133)
                     /locus_tag="VFX46_07020"
     CDS             complement(1363..2133)
                     /locus_tag="VFX46_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600368.1"
                     /translation="MAEPRTTSEPLLATKGLGRNFGGLAAVRDVTLRLHRGELHAIIG
                     PNGAGKSTLVNLLSGHLPPSSGTIHLDGVDVSGQPAWRMTRLGIGRSFQRTNLLRSLT
                     VLENVRLAAQAAAGLSAIRLLRSAASETTIVEAAREALERVGLADSQDRPAGQLSHGE
                     QRQLEIAMTLAARPRVLLLDEPLAGMGPEESERFTILLKDLAREHAVLLVEHDMDFVF
                     AVADWMTVMVDGAVLAEGLPKDVRADPQVQRAYLGGHA"
BASE COUNT          424 a          726 c          734 g          400 t
ORIGIN      
        1 cagacgaggt cgcggaacag atccaagttc gaggcttcga gctacaaccg acggtgaacc
       61 tcaagcgacc cgttgaggct gaggacggga cacaggccga agccgcattt acggttattc
      121 gtcccgcctt cggcgacttt cccgaggcgc gtgctcaggt gttgcgccat catacgcccg
      181 agcacatctg gcaaaagcgc tacctcccgc aggacaacgg cctgaccggt ctgctcgaag
      241 ttgccctggt catcgctgat ccttccgaag ccgccgagcg ctacagccgc ttcttagggc
      301 ggacggtcga acgagaggga gcctcccttc tcatcaatct agaccgtggt ggactgcgtt
      361 ttctcgatgg ccgcggggcc gcggagcatt tcggcagagt ttcgagaccg cccctgcctg
      421 ctgtgggcgc aatcacgctc acctctagcg acctcgacaa gacgcggtac tttttcttga
      481 gcaatgggct gcggcccggg gcgatcaatc ccggacatct gctgatcgac gagtccgaag
      541 cgctcggcgt gcatctgatc atcgtgccgg agtaggcgga ctggagcagc ggtgcttcca
      601 gtctaaggcg gcgaacagtt cgggtgccac tggatcacac gccaaggtgg cggtgcatca
      661 aggctggatc ggccttcagc gaagccggcg atccttcgaa gacgacctcg cccttcacga
      721 ggatcacgat acggtccgca aggctggtga tcgcatcgac cgtcttgtcg acgataagtg
      781 tcgcgacgcc ggcgttgcgg atcttcgcga tggtgctcca gatctcctcc cggatgatcg
      841 gcgccaagcc ttctgttgcc tcatcgagga tcaacagtcg cggattggcg agcaacgccc
      901 gtgcgatggc gagcatctgt tgctcgcctc cggagagctg gccgccgcga tggctgagcc
      961 gttgcgatag tcgtggaaac aggtcgagca cccgctcgcg agtccatttc gagctaccgt
     1021 cgatgccggc acgttccgca atggcgagat tctcggcgac actgagagat gcaaagatac
     1081 cgcgctcctc agggacgtac gcgagccccg cctgagcgcg gcggaaggtc ggcatcccgg
     1141 tcgcgtcctg gccgtcgatc agcacttgcc cgacccgagg cgcgacgagg cccatcagtg
     1201 ttcgaatgag ggtcgttttc cccatgccat tgcggcccat caggcccacg gcctctccgg
     1261 cgctgatcgc aagtgagacg ccgcgaagga cgtagctcga gccgtagtag gtgtgaagac
     1321 cacagccggc cagcaaaggc gtggcacctg aagggatcgc catcacgcgt gccctcccag
     1381 ataggcgcgc tggacttggg gatcggcgcg cacatctttc ggcaggccct cggcgagcac
     1441 cgcgccgtcg accatgaccg tcatccagtc ggcgacggcg aatacgaaat ccatgtcgtg
     1501 ctcgacgagg agcaccgcat gctcgcgtgc caggtccttg agcagaatgg taaaccgctc
     1561 gctctcctcg ggccccattc cggccagggg ctcatcgagc agaagcacac gcggccgcgc
     1621 tgcgagcgtc atggcgattt cgagctgacg ctgttctccg tgagacagct gcccggcagg
     1681 acggtcctgg gagtctgcaa gaccaacgcg ctcgagagcc tcgcgagcag cctctacgat
     1741 cgtggtttca ctagcggcgg aacgcagcag ccgaatagca gacagtcctg cggcagcttg
     1801 ggctgcgagg cgcacattct caagcaccgt cagggaccgg agcaaattcg tacgctggaa
     1861 cgagcggccg atgccgaggc gcgtcatgcg ccatgcaggc tggccgctca cgtcgactcc
     1921 atcgagatgg atggtcccgg agctgggcgg caaatgtcca gacagcaggt tgaccagtgt
     1981 cgacttgccc gcaccgttag ggccgatgat ggcgtgcagc tcaccccggt gcaggcgcaa
     2041 ggtcacgtcc cgcacggcag cgagaccgcc gaagttgcgg ccgagaccct tggtggccag
     2101 cagcggctcc gacgtggtgc gcggctcagc catgaggcgc gctctccgca gctggttcgg
     2161 caggtgcttt tttcggggat gccggttgca gggttggcca ttttcgcggc agtcgcagac
     2221 cgctcagtcc cttcggcagt gcgagaacga caaacaggat caccaaaccc tggacgagca
     2281 gctt
//
LOCUS       DASUMG010000425         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_537046, whole genome shotgun sequence.
ACCESSION   DASUMG010000425 DASUMG010000000
VERSION     DASUMG010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537046"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="VFX46_07025"
     CDS             <1..296
                     /locus_tag="VFX46_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX5600369.1"
                     /translation="GHTIDIYKTSFRPMKNILAGRDERMFMKLVVDRSTQKVLGVHVL
                     GPDAAEIVQMAAIALQVGATKADFDSTIALQPTAAEELVTMREKWVPPSAAAE"
     gene            432..1151
                     /locus_tag="VFX46_07030"
     CDS             432..1151
                     /locus_tag="VFX46_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX5600370.1"
                     /translation="MSANAIARASAALLFCLAALVSGQARAQNYDGAGLLRFGVFGQV
                     ANTDLDIDRPIEARGSASDTSFGGGVTFGYDYLFQNGLILGIEGDIGIDSADAERGQR
                     ELSTNFMASIRGRVGGYVSPALMLYGTAGVAFLGVDYDGLVSPATGVRFSDSDTLVGW
                     TVGGGVEWDWHGIVFFGEYLFVSYDTFTSDETFDFLDGEEVMSTIFRNEFDVDQHLFR
                     FGVKFIIGHDYRPAETYGPFK"
     gene            1230..2465
                     /locus_tag="VFX46_07035"
     CDS             1230..2465
                     /locus_tag="VFX46_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5600371.1"
                     /translation="MRSVTVVGAGVIGMCVAAYLQRMGASVTVLDPIEPGRSCSFGNA
                     GALSPTACVPVATPGILKQVPKWLADTKAPLVVRWSYLPWAAPWLMRFVRASTKREVE
                     RSAAALRALLRPVFDCYEPLLKEAGAESLIRREGQLYVYETEAAYQADAFAWELRRRH
                     GARIEVLSSQELRELEPNLAPLFTKGILLPEHGHCIDPFGLVQALADSVRRNGGTILA
                     QRVLDIEMDADRPRAVVTAAGRHAVDVLVVAAGAWSHQLARHFGHRVPLETQRGYHAM
                     LRDPSCTPRRPIMWAEKRFMATPMQAGLRFAGTVELAGLDAPPDYQRASVLLEQGKRM
                     FPSLSGGEVSRWMGHRPCLPDSVPVIGPSPHVRNVYFAFGHGHLGLSTASTTGRLVAE
                     LVMGAKPFIDPAPYRIDRF"
     gene            complement(2493..>2805)
                     /locus_tag="VFX46_07040"
     CDS             complement(2493..>2805)
                     /locus_tag="VFX46_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HEX5600372.1"
                     /translation="ALGLIIQTLVLSLYLYVAERENLFAIFRAWRPSLTAGFMGAFAS
                     QMWFLAFAVESAAKVRTLGLVEILFAQVVSRQMFKQSLASREAIGIALIVVGVVLLLN
                     V"
BASE COUNT          507 a          858 c          904 g          536 t
ORIGIN      
        1 ccgggcatac gatcgacatc tacaagacct cgttccggcc gatgaagaac atcctggccg
       61 ggcgcgacga gcgaatgttc atgaagctgg tcgtcgaccg gtcgacccag aaggttctcg
      121 gcgtgcacgt gctcggcccc gatgctgcag agatcgtgca aatggcggcc attgctctgc
      181 aagtcggtgc aaccaaggct gacttcgatt cgaccatcgc cttgcagccc accgccgccg
      241 aggagctggt gaccatgcgc gagaagtggg tgccgcccag cgccgcggcc gagtagcaaa
      301 cgccacagtg ctcgcacttt ttcaattcgc ttaacggcgg gtaaatttcc gaagccgcaa
      361 cctgtggtag cattcccgag cgagtcgccg gcgcgttcct gcaccagcgc acacgaagag
      421 gacggacggc catgtcggca aacgccatcg cccgtgcctc ggctgctctc ctgttctgct
      481 tggctgcctt ggtctcggga caagctcgag cacagaacta cgacggtgcc ggcctgctga
      541 ggttcggcgt ctttggccag gttgccaata cggacttgga catcgacagg ccaatcgagg
      601 cgagagggtc ggcctcggac acctccttcg gtggtggtgt caccttcgga tacgattatc
      661 tgttccaaaa cgggctcatc ctggggatcg aaggggacat cggcatcgat tcggcggacg
      721 ccgaacgcgg acaacgggag ctcagcacca atttcatggc gagcatccgt ggacgtgtcg
      781 gtggctacgt cagtccggcc ctgatgctct acggcacggc aggagtggcc tttctgggtg
      841 tcgactacga tggtctcgtg agcccggcaa ccggcgtacg attcagcgac tccgacactc
      901 tggtcgggtg gacggtcggc ggcggcgtcg agtgggattg gcacggcatc gttttctttg
      961 gcgaatacct cttcgtcagc tacgacacgt tcacgtccga cgagacattc gacttcctcg
     1021 atggcgaaga agtcatgtcg accattttcc gaaatgagtt cgacgtcgat cagcacctct
     1081 tccggttcgg cgtaaagttc atcattggcc atgactaccg gccggccgag acctatgggc
     1141 cgtttaagta gctgacaact caagtgcgat cagccaagct cttcgccagc gacaagctcg
     1201 aaggcgcgga ggcgtaaggc tgtaacttga tgcggagtgt cactgtcgtc ggcgccggtg
     1261 tcattgggat gtgcgttgca gcctacctgc aacgcatggg ggccagcgtg actgtgctcg
     1321 acccgatcga gccagggcgg agctgttcct tcggcaacgc cggtgcgctc agccccacgg
     1381 cctgcgtgcc cgtcgccacg ccgggtattc tcaagcaggt gccgaagtgg ctcgcggaca
     1441 caaaggcgcc gcttgtggtg cgctggtcct atctgccgtg ggcggcgccg tggctgatgc
     1501 gcttcgttcg ggcaagcact aagcgcgaag tggagcgcag cgctgctgca ctcagagccc
     1561 tgcttcgccc tgtctttgat tgctacgagc ctctactgaa ggaagcggga gccgagagcc
     1621 tcatccggcg ggagggccag ctttacgttt atgagacaga agcggcctac caggctgacg
     1681 cttttgcttg ggagctgcga cgccggcatg gtgcgcggat cgaggttctg tcctcgcaag
     1741 agctgcgcga gctcgagccg aacctggcgc cgcttttcac caagggaatc ctgctgccgg
     1801 agcacggtca ttgcatcgac ccgttcgggc tcgttcaggc gctagccgac agtgtgcggc
     1861 gtaacggcgg caccattctc gctcaacgcg tgctcgacat cgagatggat gccgacaggc
     1921 cgcgggcggt cgtgacggcg gcaggccggc atgcggttga tgttctggtg gttgctgccg
     1981 gcgcgtggtc tcatcagctc gcccgccatt tcggccacag ggtgcccctt gaaacgcaac
     2041 gcggctatca cgccatgctg cgtgatccga gctgcacgcc gcgccggccc atcatgtggg
     2101 ccgagaagag gtttatggcg acgccgatgc aggcgggatt gcggttcgca ggcaccgtcg
     2161 agcttgcggg gctcgatgcg ccgcctgatt accagcgcgc cagcgtgctc ttggagcaag
     2221 ggaagcggat gttccctagc ctaagtggcg gggaggtcag ccgctggatg ggccatcggc
     2281 cgtgcctgcc ggactcagtt ccggtgatcg gtccttcccc tcacgtgagg aatgtctatt
     2341 tcgctttcgg ccacggtcac ctggggctgt ccacggcttc gacgacgggg cggctcgtgg
     2401 cggagcttgt catgggcgcc aagcccttca tcgatccggc accttaccgc atcgacaggt
     2461 tctaagcgct caacgaagtc gtttgttggg cttcaaacgt tcaacagcag cacgactccg
     2521 acgacgatca gtgcaatccc gatagcttcg cgcgaggcga ggctctgctt gaacatctgc
     2581 cgcgagacca cctgcgcgaa caggatctcg acgagcccga gcgtgcgcac cttggcggca
     2641 ctctccaccg cgaacgcgag gaaccacatc tgcgaggcga aggcacccat gaagcctgcc
     2701 gtcagggatg ggcgccaggc acggaaaatg gcgaacagat tttcgcgctc agccacgtac
     2761 aggtagagcg acagcaccag cgtctgaatg atgagcccga gcgcc
//
LOCUS       DASUMG010000426         4379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_938662, whole genome shotgun sequence.
ACCESSION   DASUMG010000426 DASUMG010000000
VERSION     DASUMG010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938662"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFX46_07045"
     CDS             <1..205
                     /locus_tag="VFX46_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600373.1"
                     /translation="ASCLVFWTCSLREAFADLDTMWNAAVGNACGASATYFKNVSAVT
                     CNAPSARIINEFAELEPKSSPLI"
     gene            792..1985
                     /locus_tag="VFX46_07050"
     CDS             792..1985
                     /locus_tag="VFX46_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600374.1"
                     /translation="MAIRKAKGVSRRGVLKGAAAVTALSAVPTGWAIAQKRPLKIGLM
                     LPYSGTYAKLGENITYGVEMVIAEKGGKLGGREVELIKLDDESKPELAPQNADRLVKR
                     DQVDVLIGTVHSGVQMGIHRVVRESGTLTIIPNAGNVAITRELCARNVYRASFSNWQP
                     AYGIGLAAGKKGYKRGIWVSWDYAAGAESCEGFKQGLEEQGGTFVEFLKLPFPQTNFQ
                     PLLAQIPGLEVDIVGAFFAGGGAVQFVKEYAAAGLKVPLCGNGFLTEGTLQAQGEAAE
                     GIETALHYGDGLDNPKNIAFRKAFLEKAGREADVYAVQGYDSAQLLAVGLEAVNGDIE
                     DEENLHKALESAKIDSPRGPMSMSPSHEVIHNMYLRKVTNGQNKVIGIAAEQLADPGT
                     GCKMA"
     gene            2114..2986
                     /locus_tag="VFX46_07055"
     CDS             2114..2986
                     /locus_tag="VFX46_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5600375.1"
                     /translation="MDIAGFLAQLLNGVQYGLLLFLIASGLTLIFGVMGIINLAHGSL
                     FMVGAYTAFVVTSATGSLWIALPAGIVGGIVLGLLLERFLFRYFYHREHLDQVLLTFA
                     LILLFEEGRSLLVGNDFHSVPVPAALDFSIPITEGFSYSAYRFAVIGICLLLALLMFF
                     WIERTKMGAMIRAAAEKPEMVDVLGIDARRIHLTVFAVGTALALTAGVLAAPLYSVYP
                     NMGDGFLIISFVVVVIGGLGSVSGAFWSALLIGLVDTLGKAYLPVVAGLSVYVLMAAV
                     LLWRPSGIFGPRSP"
     gene            3008..4027
                     /locus_tag="VFX46_07060"
     CDS             3008..4027
                     /locus_tag="VFX46_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX5600376.1"
                     /translation="MLPPSKYGWMGLAAGALALAALPFAVPSYYVELGTYALIAAMLA
                     LSLQLLVGCTGLVSLGHGAFYGLAAYTVYLITPENQGLSIFVTLPAAVLAAGLAAFVV
                     GALSLRTRGFFFLMVTLAFGQMVFFLFHDTKIGGGADGAFLARPVLSLFGFELPLTRR
                     QRPIAIYYVALAQMVLMYLGLVFLLRSLFGRVLEGIRVNEHRVQAMGYDTYRYKLAAF
                     VIAGMLAGVAGHMWAMHRGFVNPELVAWHRSAEALMMILLGGLNTLHGPILGALAYTG
                     LEELAQIVTERKLLVQGLVILFVVLALPKGLSGLRLPRRWPMLQPAAPKKTPTEPAAE
                     SVPHG"
     gene            4020..>4379
                     /locus_tag="VFX46_07065"
     CDS             4020..>4379
                     /locus_tag="VFX46_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600377.1"
                     /translation="MAEPRTTSEPLLATKKLCRNFGGLAAVRDVSLSLHRGELHAVIG
                     PNGAGKSTLVNLLSGHLQPSSGTIHLDGVDVSGQPAWRMTRLGVGRSFQRTNLLRSLT
                     VLENVRLAAQAAAGLPTI"
BASE COUNT          738 a         1333 c         1362 g          946 t
ORIGIN      
        1 cgcctcgtgc ctcgtatttt ggacgtgctc gttgcgcgag gcctttgctg atctcgatac
       61 catgtggaat gcagccgtcg ggaatgcgtg tggagcctcc gcaacctatt tcaagaacgt
      121 ttccgccgtt acctgcaacg cgccttctgc gcgcatcatc aacgaatttg ccgagcttga
      181 gccgaaatcg agtccactga tctagtttcg accacctttg agctttgcgg cgctcggact
      241 ccgccgtttt cgttttttcc caagccagtt cctcgcttaa tgcctcgtac tctggcactt
      301 cgtgcaaaaa gacaaatccg catccctcgc actcgacaac gggccattca gtgactgtgt
      361 attgcgacag caatcgagca ccggtgctac cgcatacgtc acaatggcgc cttcttgtct
      421 gaaccttcat gtccgctccc tgcagtgccc gtctgacacg atcaagaccc tctggcttag
      481 tctcaacaga tcgcaatttc aaacatcgtt cgtcacatac cgcccaggaa tgtgggatct
      541 cgcgttgagc tgtccgggtc cacggattgc ggtgccgtgg ggccaacatt cctcgatgta
      601 ccgccagctg gtaaatcagg ttcacgggct cttcgctcgc aaccatcttg cgctcagccg
      661 cgccgtatgc gagcgagggg ccgagggcgg ctcgccgcga gttgcgcgcg cgggctatgt
      721 ctcggtttga aattggtgtt cgtttttccc ggggcgatgg tagggtccgg ggggagcaaa
      781 gggaggaagc catggccatc aggaaggcga aaggcgtttc gcgccggggc gttttgaagg
      841 gtgcagcagc cgtaacggcg ctgtcagcag tgccgacggg ctgggcaatc gcacagaagc
      901 ggccgctgaa gatcggcctc atgctgccct actccggcac ctacgccaag ctcggcgaga
      961 acatcaccta cggcgtggag atggtcatcg ccgaaaaggg cggaaagctc ggcggccgcg
     1021 aggtggagct catcaagctc gatgacgagt cgaagccgga gctcgcccct cagaacgcag
     1081 atcgcctcgt gaagcgcgac caggtggatg tgctgatcgg caccgtgcat tcaggcgtgc
     1141 agatgggcat ccatcgcgtc gtccgcgaga gcggcacgct gacgatcatt cccaacgccg
     1201 gcaatgtggc tatcacacgc gagctgtgcg cgcgcaacgt ctaccgcgcc tcgttctcca
     1261 actggcagcc cgcctatggc atcggtctcg cggccggcaa gaaaggctac aagagaggca
     1321 tctgggtcag ctgggattat gccgccggtg ccgagtcctg cgagggcttc aagcagggcc
     1381 ttgaggagca agggggaacg ttcgtcgagt ttctcaagct gcccttcccg caaacgaact
     1441 tccagccgct gctcgcgcag atccctgggc tagaagtgga catcgtgggc gccttcttcg
     1501 ccggtggcgg ggcggtgcag tttgtgaagg agtacgcagc tgcgggcctc aaggtgccgc
     1561 tgtgcggcaa tggcttcctg accgagggca cgttgcaggc acagggcgag gccgccgagg
     1621 gcatcgagac tgcgctgcac tacggcgacg ggctcgacaa tccgaagaac atcgcattcc
     1681 gcaaggcctt cctggagaag gcaggccgtg aggcggatgt ctacgccgtg cagggctatg
     1741 actcggcgca gctgctcgcg gtcggccttg aggcggtgaa cggcgatatc gaggacgagg
     1801 agaatctgca caaggctctg gagagcgcaa aaatcgacag cccgcgtggt ccgatgtcga
     1861 tgtcgccctc acacgaggta attcacaaca tgtatctgcg gaaggtgaca aacggccaga
     1921 acaaggtgat cggcattgcg gccgagcagc tggccgaccc tggcaccggc tgcaagatgg
     1981 cttgaagcgg ggggcgaatg gtgactggcg gatagcacat ctagaagatc gatttggtcc
     2041 ggcagcggag caaacctcgc tccctgcctt cgctatccgc ctcccctatt tcctattcgc
     2101 agtcttttca gtgatggata tcgccggctt tctcgcccag ctcctcaacg gtgtgcagta
     2161 cgggctgctg ctgttcctga tcgccagcgg cctgacgctg atcttcggcg tgatgggcat
     2221 catcaatctg gcccacggct cattgttcat ggtgggagcc tatacggcgt tcgtggtgac
     2281 gagcgctacg ggctcgctgt ggatcgcatt gccggccggg atcgttggcg ggatcgtgct
     2341 cgggttgctg ctcgaacgct tcctctttcg gtacttctat catcgcgagc atctcgatca
     2401 ggtgctgctg accttcgctc tcatattgct attcgaggaa ggccgctcac tgctcgtcgg
     2461 caacgacttc cattccgtgc cagtgcctgc cgcgctcgat ttctccattc ctatcactga
     2521 gggatttagc tactcggcct accggtttgc cgtgatcggc atctgcctgc tgctcgcact
     2581 actgatgttt ttctggatcg agcggacgaa aatgggagcc atgatccgcg ccgcggcgga
     2641 aaagccggaa atggtcgacg tgctcggcat cgacgcgcgg cgaattcatc tcaccgtatt
     2701 cgccgtcgga actgcgctgg cgttgacggc gggcgtgctg gcggcacccc tctattccgt
     2761 ctaccccaat atgggcgacg gatttctcat catctctttc gttgtcgtcg tgatcggagg
     2821 gttgggctcg gtttccggag ccttttggtc ggcgctgctg atcgggctcg tcgacacgct
     2881 tggcaaagcc tacctgccgg tcgtcgcggg tctctccgtc tatgtgctga tggcagctgt
     2941 gctgctgtgg cggccttccg ggattttcgg cccgaggtca ccttaatccg ggcgtcagga
     3001 acagccgatg ctgcctccgt cgaaatacgg ttggatgggc cttgcagcgg gggcgctagc
     3061 gctcgcggct ttaccgtttg ccgtgccctc ctattacgtc gagcttggca cctatgcgct
     3121 gatcgcagcc atgctcgccc tcagcctgca gctgcttgtt ggctgcacgg ggctcgtcag
     3181 cctggggcac ggtgcttttt acgggctcgc ggcctacacc gtttacctca taacacccga
     3241 aaatcagggc ctgtcgatat tcgtgacatt gcctgcagct gtgctggctg ctggtctcgc
     3301 ggcgttcgtc gtcggagcgc tatctctccg gacgcgtggc ttcttcttcc tcatggtgac
     3361 gctggcgttc gggcagatgg tgttctttct gttccatgac acgaagatcg gcggcggtgc
     3421 cgacggtgcc ttcctcgccc ggccagtgtt gtcgctgttc ggcttcgagc ttccgctgac
     3481 gcgccgccag cgaccgattg cgatctacta cgtcgccctc gcgcagatgg tgctgatgta
     3541 ccttggcctg gtgttcctgc tccgctcgct tttcggccgc gttctcgaag gtatccgcgt
     3601 caatgagcat cgcgtgcagg cgatgggcta cgatacctac cgctataaac ttgcggcctt
     3661 cgtgatcgcc ggaatgctgg cgggcgttgc ggggcatatg tgggccatgc accgcggctt
     3721 cgtgaacccc gagctcgtcg cgtggcatcg ctcggcagag gcgctcatga tgatcctgct
     3781 cggcggtctg aacactctgc atggtccgat tttgggggcc ttggcctaca cggggttgga
     3841 ggagctggct cagatcgtca cggaacgtaa gctgctcgtc caaggcttgg tgatcctgtt
     3901 cgtcgttctc gcactgccca aagggctgag cggtctgcgg ctaccacgca gatggccaat
     3961 gctgcagccg gcagctccga aaaagacacc gaccgaaccc gcagcggaga gcgtgcctca
     4021 tggctgagcc gcgcacgacg tcagagccgc tgctggccac caagaagctc tgccgcaact
     4081 ttggcggcct cgccgccgta cgggacgtgt ctctcagctt gcaccggggc gagctgcacg
     4141 ccgtcatcgg cccaaacggc gcgggcaagt cgacattggt caatttgctg tctgggcacc
     4201 tgcagcccag ctcagggacg atccatctcg acggggttga tgtgagcggg cagcctgcat
     4261 ggcgcatgac gcgccttggc gtcggccgct cgtttcaacg cacaaatctc ctgcggtctt
     4321 tgaccgtgct cgagaacgtg cgtctcgcag cccaggctgc cgcgggacta cccaccatc
//
LOCUS       DASUMG010000427         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_55489, whole genome shotgun sequence.
ACCESSION   DASUMG010000427 DASUMG010000000
VERSION     DASUMG010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55489"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFX46_07070"
     CDS             complement(<1..615)
                     /locus_tag="VFX46_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5600378.1"
                     /translation="MNVHDSASGKADTKTATLTYNGKQVTLPVRSGSIGPDVIDVTRL
                     YRETGCFTYDPGFTSTANCSSRITYIDGENGILLYRGYPIEQLAEQSTFIEVCHLLLY
                     GELPTKQQLEEFRNTITRHTMVHEQMTRFFTGFRRDAHPMAIMVGTVGALSAFYHDST
                     DISDPVQRMIASHRMIAKMPTIAAMAFKYHVGQPFVYPLNALDYA"
     gene            complement(843..2252)
                     /gene="gltX"
                     /locus_tag="VFX46_07075"
     CDS             complement(843..2252)
                     /gene="gltX"
                     /locus_tag="VFX46_07075"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX5600379.1"
                     /translation="MVTRFAPSPTGFLHIGGARTALFNWLYAKHTGGKFLLRIEDTDR
                     ERSTKEAIDAILDGLKWLELDWDDEPVFQSTRVDRHREVVEELLRTGNAYRCYCTPEE
                     LEAMRKKAEAEGRPPVYNGYWRDRDPSEAPPGVKPAIRIKTPRTGETVIEDRVQGRVV
                     FQNEHLDDLIILRSDGTPTYNLAVVVDDHDMGVTHIIRGVDHLTNAARQMQIYRALGW
                     EVPVFAHVPLIHGPDGAKLSKRHGALGVEAYRAMGYLPAALRNYLVRLGWSHGDDEIF
                     TTEQMIEWFDLDGIGRSPARFDFAKLEDLNGYYIRNTPDDELLERTKQALPEMENGRE
                     LEARFASAGWEKLAAALPSLKTRSKTLKELVDGAAFIIAERPLALDEKATKLLTEDAR
                     KVLGRLLPRLEAAEWTASALETEVRSFAEAEGLKLGGVAQPLRAALTGRSVSPPVFDV
                     MAVLGRDEALARIRDQALA"
BASE COUNT          391 a          701 c          743 g          427 t
ORIGIN      
        1 ggcgtagtcg agcgcgttga gcggatacac gaacggctgg ccgacgtggt acttgaaggc
       61 catcgccgca atcgtcggca tcttggcgat catccgatgc gaggcgatca tgcgctgcac
      121 cggatcgctg atgtccgtcg agtcatggta gaaggccgat agggcaccga ccgtcccgac
      181 catgatggcc atcgggtgcg cgtcccgccg gaaaccggtg aagaaccggg tcatctgctc
      241 atgcaccatc gtgtggcggg tgatggtgtt acggaattcc tccagctgct gcttcgtcgg
      301 cagctcaccg taaagcagta ggtggcaaac ctcgatgaag gtcgactgct ccgccagctg
      361 ctcgatcggg tagccgcgat aaaggagtat gccgttctcg ccgtcgatgt acgtgatgcg
      421 ggacgagcaa ttggcggtcg acgtgaagcc cggatcgtag gtgaagcagc cggtctctcg
      481 gtaaaggcga gtgacgtcga tgacatccgg ccctatcgag ccagagcgga ccggcaaagt
      541 cacctgcttg ccattgtagg tcagagtcgc ggttttcgtg tcggccttgc ccgacgcgct
      601 gtcgtgcacg ttcatcgcca atcctttcta tcgtctcgac gcctgccccg tacgcccacc
      661 gtcgaggagc gcggcacgct tcggcagcct ggcgccacct gctattacac atttcgcgct
      721 gcgacaagac ggccaatggg cgaaacttgc agcttcgagt tcacgcgaac gacaccgaac
      781 aacgtccgag gattacggcg gttcgcgctc ccacgggctg gatcgcagca agcggttcgt
      841 ccttaagcca gtgcttgatc ccggatccgc gcgagggcct catcgcggcc cagcacggcc
      901 atcacgtcaa acactggcgg agacacggag cggccggtga gcgccgccct gagcggttgt
      961 gccacgccac caagcttaag gccttcggct tccgcgaagc tgcgcacttc ggtttcgagc
     1021 gcgctggcgg tccactcggc tgcttcgagc cggggaagca atcgcccgag caccttgcga
     1081 gcgtcctcgg tgagaagctt tgtggctttt tcatccaggg caagcgggcg ctcggcgatg
     1141 atgaaggccg cgccgtcgac caactccttc agtgttttcg agcgcgtttt caggctgggt
     1201 aaggcggcgg caagcttctc ccagccggcc gacgcgaatc gcgcctcgag ctcacggccg
     1261 ttctccatct ccggtagagc ctgcttggtt cgttcgagca gctcgtcgtc gggcgtgttg
     1321 cggatgtagt agccgttcag gtcctcgagc ttggcaaagt cgaagcgcgc aggggagcgg
     1381 ccgatgccat cgaggtcgaa ccactcgatc atctgctccg tcgtgaaaat ctcgtcatca
     1441 ccgtggctcc agccgaggcg cacgagatag ttgcgcaaag ccgcaggcag gtagcccatc
     1501 gcccggtacg cctcgacgcc cagtgcaccg tgacgcttgg agagcttggc gccatcgggg
     1561 ccatggatca gcgggacgtg ggcgaagacg ggcacctccc aacccagggc gcggtagatt
     1621 tgcatctggc gcgcggcatt ggtgaggtga tccacgccgc ggatgatgtg ggtgacgccc
     1681 atgtcgtggt catcgacgac gaccgcgaga ttgtacgttg gcgtcccgtc gctgcgcagg
     1741 atgatgaggt cgtccaggtg ctcgttctgg aacacgaccc ggccctgcac ccgatcctca
     1801 atgacggtct ctccagtgcg cggcgtcttg atgcgaatgg ccggcttcac accgggaggc
     1861 gcctcggagg ggtcgcggtc gcgccagtag ccgttgtaga cggggggccg gccctctgcc
     1921 tcggccttct tgcgcatggc ctcgagctcc tcgggggtgc agtagcaccg ataggcattg
     1981 cccgtacgga gcagctcctc gacgacctcg cggtggcggt cgacccgggt cgattggaag
     2041 accggctcat cgtcccaatc gagctccagc cacttcaacc catcgagaat ggcgtcgatt
     2101 gcctccttgg tcgaccgttc gcggtcggta tcctcgatgc gcagcaggaa tttcccgccc
     2161 gtgtgcttgg catagagcca gttgaagagg gcggtccgcg cgccaccgat gtgcaggaag
     2221 ccggttggcg agggggcgaa acgcgtaacg atgctggatc cg
//
LOCUS       DASUMG010000428         2158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1098986, whole genome shotgun sequence.
ACCESSION   DASUMG010000428 DASUMG010000000
VERSION     DASUMG010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098986"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VFX46_07080"
     CDS             <1..191
                     /locus_tag="VFX46_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEX5600380.1"
                     /translation="ISMGSCANGGGYYHYSYAVVRGCDRIVPVDVYVPGCPPTAEALL
                     YGMLLLQRKIRRTGTIER"
     gene            227..838
                     /locus_tag="VFX46_07085"
     CDS             227..838
                     /locus_tag="VFX46_07085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5600381.1"
                     /translation="MAGDNESLRELGEYLQGKLGSTLQRWDVQLGELTIYVDAEQIVP
                     VMKLLRDDARCRFEILIDLCGVDWPGREKRFDVVYHLLSPRLNQRIRVKIEADEVNPV
                     ASVVDVFPTANWYEREAYDMYGILFSGHPDLRRILTDYGFQGFPLRKDFPLSGYVEVR
                     YDDEQKRVVYEPVRLTQEFRDFDFESPWEGTGRILPGDEKAGS"
     gene            854..2044
                     /locus_tag="VFX46_07090"
     CDS             854..2044
                     /locus_tag="VFX46_07090"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5600382.1"
                     /translation="MPETDIRPFTINFGPQHPAAHGVLRLVLELDGEVVTRVDPHIGL
                     LHRGTEKLIETRTYLQNIGYFDRLDYVAPMNQEHAFCLAVEKLVGIEVPYRAQLIRVL
                     YSEIGRLLSHLLNVTTQAMDVGALTPPLWGFEEREKLMIFYERASGARMHAKYFRFGG
                     VKQDLPPELIEDIAKFCETHPKVLDDIHDLVMENRIFKQRNVDIGVVSLEDCMKWGFT
                     GVMLRGSGAAWDLRKAQPYECYAELDFDIPVGKNGDNYDRQLIRMEEMRQSVRIMKQC
                     CEKLLSAEGQGPHISEDRKVVPPKRADMKRSMEALIHHFKLYTEGIRVPKGEVYAAVE
                     APKGEFGVYLVADGSNKPYRCKIRAPGFAHLQAMDFMNRGHMLADVSAILGSLDIVFG
                     EIDR"
BASE COUNT          400 a          661 c          691 g          406 t
ORIGIN      
        1 tcatctctat gggcagctgc gccaacggcg gcggctacta tcactactcc tacgccgtcg
       61 ttcgcggttg tgatcgcatc gttcccgttg atgtctatgt gcccggctgc ccgccgacag
      121 cggaggcgtt gctttacggc atgctcctgc tgcagaggaa aatccgccgc accggcacca
      181 tcgagcgctg agtggccacg ccagccggag ggggagaaag gtcgacatgg cgggtgacaa
      241 cgaatcactg cgggagctcg gcgaatacct gcaaggcaag ctgggctcca ccttacagcg
      301 ctgggatgtg cagctcggcg agctgacgat ctacgtcgac gcggagcaaa tcgtgccggt
      361 catgaagctg ctgcgggatg atgcccgctg ccgcttcgag atcctgatcg acctgtgtgg
      421 tgtcgactgg ccgggccggg agaagcgctt tgacgttgtc taccacctgc tgtcgccgcg
      481 gctgaaccag cgcatccgcg tcaagatcga ggccgatgag gtcaatcccg tcgctagtgt
      541 cgtcgatgtc tttccgacgg ccaactggta tgagcgggaa gcctacgaca tgtacggcat
      601 tctgttctcc ggccatccgg acctgcgccg cattctgact gactacgggt tccagggctt
      661 ccccttgcgc aaggacttcc cgctcagcgg ctacgtcgag gtccgctatg acgatgagca
      721 gaagcgcgtc gtctacgagc cggtccgtct gacgcaagaa ttccgcgatt tcgatttcga
      781 gagcccctgg gagggaaccg gaagaatact gccgggggac gagaaggctg gaagctagcg
      841 cctcgagagc cagatgccag aaaccgatat tcgcccattc accatcaact tcggcccgca
      901 gcacccggct gcgcacggcg tgctgcgcct cgtgctggaa ctggatggtg aggtcgtcac
      961 gcgggtcgat cctcatatcg ggctgctgca ccgcggcacc gagaagctga tcgagacgcg
     1021 gacctatctg caaaacatcg gctactttga ccgcctcgac tacgtggctc cgatgaacca
     1081 ggagcacgcg ttctgtctcg cggtcgagaa gctggtcggg atcgaggtgc cgtaccgcgc
     1141 tcagctgatc cgggtgctct actccgagat cggccggctg ctctcgcatc ttctcaacgt
     1201 caccacgcag gcgatggacg tgggtgcgct cacgccgccg ctgtgggggt tcgaggagcg
     1261 cgagaagctc atgatcttct acgagcgtgc ctcgggcgcc cgcatgcacg ccaagtactt
     1321 ccgcttcggc ggggttaagc aggacctgcc gccggagctg atcgaggaca tcgccaagtt
     1381 ctgcgagacg caccccaagg ttctcgacga catccacgac ctcgtcatgg agaaccgcat
     1441 cttcaagcag cgcaacgtcg acatcggcgt cgtctcgctg gaagactgca tgaagtgggg
     1501 gttcaccgga gtgatgctgc gcggctccgg cgcggcgtgg gatctgcgca aggcccagcc
     1561 ctacgaatgc tacgcggagc tcgatttcga cattccggtc ggtaagaacg gcgacaatta
     1621 cgaccggcag ctcatccgca tggaggagat gcgccagtcc gtgcggatca tgaaacagtg
     1681 ctgcgagaag ctgctgtctg ccgaggggca gggcccgcac atctccgaag accgcaaggt
     1741 ggtgccgccg aagcgggcgg atatgaagcg ctcgatggag gcgctgatcc atcacttcaa
     1801 gctctacacc gagggcatcc gcgtgccgaa gggtgaggtt tacgccgcgg tggaagcgcc
     1861 caagggtgaa ttcggcgttt atctggtcgc ggatggcagc aacaagcctt atcgctgcaa
     1921 aatccgtgcc cctggctttg cccacctgca ggccatggac ttcatgaacc ggggccacat
     1981 gctggctgac gtctcggcga tccttggctc gctggatatc gtgttcggcg aaatcgaccg
     2041 gtgatgggga cttggcgggt ggacccactc ctcaagtcat cccggcgctt gtcgccggga
     2101 tccactctgc ctcttgctcc ggagcttgcg gataggtgga tcccgggcac aaggcccg
//
LOCUS       DASUMG010000429         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1180191, whole genome shotgun sequence.
ACCESSION   DASUMG010000429 DASUMG010000000
VERSION     DASUMG010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180191"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VFX46_07095"
     CDS             <1..558
                     /locus_tag="VFX46_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600383.1"
                     /translation="MLAILDTTPAVFVAIAIAVLMIAASVFIIFYHDRKHEELDQWVE
                     FSFSRETLRNAAMYYRFMAVSMLVFYVLFTISCLLLQAEGHMIFSDGKEPIHAGPIAA
                     SLFTIDLVLRGGFFDIMEHFDLGVSPISMNRKMFWFVIYAFVFRMFYAVTLIKILISF
                     VWIYGKIRMARQTFGSSSSQLRLFE"
     gene            660..>2506
                     /gene="pheT"
                     /locus_tag="VFX46_07100"
     CDS             660..>2506
                     /gene="pheT"
                     /locus_tag="VFX46_07100"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX5600384.1"
                     /translation="MKFTLSWLKDHLDTNASLEELADKLSAIGLEVESIDNPAEKLAP
                     FTVARVLEAKQHPNADRLRVCQVEVAPGQPPVEVVCGAPNARTGMIGVFAPIGTYIPG
                     TGITLEKRSVRGVVSNGMLVSERELELSDDHEGIIELDPALAEHVGKRYIDVVGLNDP
                     VIEVKLTPNRPDCTGVRGIARDLAAAGLGTLKPEPEIGNVEGTFDCPIEIRLEFTPET
                     ASACPVFAGRLVKGVKNGPSPAWMQKRLKAAGLRPISALVDVTNYISLDRGRPLHVYD
                     ADKLKGAIRARLGRPGERFLALDGKEYEVDETMCVIADDSGPLGLGGIIGGESSACTE
                     ETTNVFIESAYFDPVRTAATGRKAGVQTDARYRFERGVDPAYVVPGLDYATDLILKLA
                     GGKPSKRSVAGTPPEGRMTFAFDFRRVARLTGLEVDDTVSRKVLTDLGFEVEGTGLEV
                     QVTRPSWRPDVHGPADLVEEVVRIIGLDKVPSTPIPRTHGVTRTVLTEAQRRARRARR
                     VLAGRGLIEAITWSFIPRRHSELFGGGLETLELANPISVEMSSMRPSLLPGLLTAAQR
                     NRNRGFDDLALFELGQAYRGDKPEDQIQIAAGVRAGAARLAGVGRHWSEP"
BASE COUNT          440 a          806 c          791 g          469 t
ORIGIN      
        1 atgctcgcga tcctagacac gacgccggcc gtgtttgtgg ccattgccat cgccgtgctg
       61 atgatcgcgg cgtcggtttt catcattttc taccacgacc gcaagcacga agagctcgac
      121 cagtgggtcg aattctcctt cagccgtgag acgcttcgga atgcggccat gtactaccgc
      181 ttcatggccg tttcgatgct ggtgttctac gtgctcttca cgatcagctg cctgctgctc
      241 caggcggaag ggcacatgat cttctcagac ggcaaggagc ccattcatgc cggcccgatc
      301 gccgcctccc tcttcaccat cgatctcgtg ctgcgcggcg ggttcttcga catcatggag
      361 catttcgatc tgggcgtctc gccgatctcc atgaaccgga agatgttctg gttcgtgatc
      421 tacgccttcg tgtttcgcat gttctatgcg gtgacgctca tcaagatcct gatctcgttc
      481 gtctggattt acgggaagat ccggatggcg cgacaaacgt tcggctcgag cagcagccag
      541 cttcggctat tcgagtgacg taacccttgg cggcggcccc gcaatcgggg taagacgcac
      601 acgccaaatg cgcccggata tgccgggaaa ccttccaaac ccgcgggacg aaggacgcaa
      661 tgaaattcac tctgtcctgg ctcaaggacc atctcgatac gaacgcctcg cttgaagagc
      721 ttgcggacaa gctgtctgcg attgggctcg aggttgaaag catcgacaat cccgcggaga
      781 agctggcgcc gtttaccgtc gcgcgcgtcc tcgaggccaa acagcatccg aatgcggatc
      841 gcctgcgcgt gtgccaggtc gaggttgcgc caggccagcc gccggtcgag gtcgtctgcg
      901 gcgcgcccaa cgcccggacc ggcatgatcg gcgtgttcgc gcccatcggc acctacatcc
      961 ccggcaccgg catcactttg gaaaagcgca gcgttcgcgg ggtcgtgtcg aacgggatgc
     1021 tcgtgtccga gcgggagctg gagctgtccg acgatcacga aggcatcatt gagctagacc
     1081 ccgcgttggc cgagcacgtc ggcaagcggt acatcgacgt cgtcgggctc aacgatccgg
     1141 tcatcgaggt taagctcaca cccaaccgtc ctgactgcac cggtgtgcgc ggcatcgcgc
     1201 gcgacctcgc cgctgccggc ctcggcacgc ttaagccgga accagaaatc ggcaatgtcg
     1261 aggggacctt cgactgcccc atcgaaatcc ggctcgagtt cacgcctgaa accgcgagcg
     1321 cgtgcccggt gttcgctggc cggcttgtga aaggcgtgaa gaacggcccc tcgccagcat
     1381 ggatgcagaa gcggctgaaa gctgccggcc tccggcccat cagcgcgctg gtcgacgtca
     1441 ccaactacat ctcactcgac cgcggccgcc cgctccacgt ctacgatgcc gacaagctca
     1501 agggcgccat tcgggcccgg ctcggccgtc cgggcgagcg tttcctggcg ctcgacggca
     1561 aggagtacga ggtcgacgag accatgtgcg tgatcgccga cgacagcggt ccgctgggtc
     1621 ttggcggcat catcggcggc gagagcagcg cctgcaccga ggagaccacc aacgtcttca
     1681 tcgagagcgc ctacttcgat ccagtgcgca cggctgccac aggccgcaag gcgggcgtac
     1741 agaccgatgc gcgctatcgg ttcgagcgcg gggtcgatcc ggcctatgtc gtgcccgggc
     1801 tcgactacgc gaccgatctc atcctgaagc tcgccggcgg taagccgtca aagcggagcg
     1861 ttgctggtac gccgcctgag ggtcggatga cattcgcctt cgattttcgc cgtgtggcgc
     1921 gccttaccgg tctcgaggtt gatgacaccg ttagccgtaa ggtgctgacg gatctcgggt
     1981 tcgaggtcga aggcactggt ctcgaggttc aagtgaccag accgagctgg cgacccgacg
     2041 tgcatgggcc ggcggatctg gtcgaggagg ttgtgcgcat catcggtctc gacaaggtgc
     2101 cctcgacgcc catcccgcgc acgcacggtg tgacgcgcac cgtactgaca gaagcgcagc
     2161 gtcgcgcgcg ccgggcgcgc cgcgttctcg ccggccgcgg tctgattgaa gcgatcacct
     2221 ggtccttcat tccgcgacgg cattcggagc tgttcggtgg agggctggag accctagaac
     2281 tggcgaaccc gatttccgtc gagatgtcct cgatgcgccc gagcttgttg cccgggctgc
     2341 tgacggcggc gcaacgcaat cgcaaccgcg gcttcgacga tctcgcgctg ttcgagctcg
     2401 gccaagccta tcgtggcgac aaaccggagg atcagatcca gatcgcagca ggcgtgcgcg
     2461 ccggtgccgc tcgtctggcc ggagttggcc gccattggtc cgagcc
//
LOCUS       DASUMG010000430         4312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_617515, whole genome shotgun sequence.
ACCESSION   DASUMG010000430 DASUMG010000000
VERSION     DASUMG010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617515"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFX46_07105"
     CDS             <1..397
                     /locus_tag="VFX46_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600385.1"
                     /translation="LGLFQMTLVTRLVRSEMLEVLRTDYIKFARARGLTSRAIHFGHA
                     LKNTLVPVITIIGLQVGALIAFAIITETVFQWPGMGLLFIQSVQNADIPVMSAYLMLV
                     AFLFVLINFIVDLLYVAIDPRIRIVGGAS"
     gene            402..1364
                     /locus_tag="VFX46_07110"
     CDS             402..1364
                     /locus_tag="VFX46_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600386.1"
                     /translation="MAVEAEGTNGTGNPGEEQGVVARILDSDLLHSFLRNRLVVVAAI
                     VTLAFVLVSLLAPWIAPHDPYDVRTLNLLDSHTPPVWEEGGDPRFLLGTDDQGRDILS
                     TILFGARSSIAIGILSVLLAVAIGVTLGLIAGYFGGPIDTIIMRIADVQLTFPAILTA
                     LLIDGVARVLFGTHGSGGGEFWILVVSIGLSFWVQYARTVRGITLVERNKEYVQAARL
                     IGLSSFNIMFNQVLPNVMSPVLVIATINLALAILTEATLSFLGVGLPPTEPSLGTLIR
                     IGNNFLMSGEYWIVIFPGIALAALVLAVNLLGDWLRDALNPKLR"
     gene            1380..2366
                     /locus_tag="VFX46_07115"
     CDS             1380..2366
                     /locus_tag="VFX46_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600387.1"
                     /translation="MSTPVLSVRHLRVEFPTRKGVLVAVDDVSFDIAEGEVLGVVGES
                     GAGKSMTGSAVIGLIEPPGRIAGGEVWLKGQRIDNLPYEDMRRIRGRRIGMIFQDPLT
                     SLNPLYRIGDQLIETIRTHLPVSEGEARKRAIKLLTDVGIQGAEARINAYPHQFSGGM
                     RQRVVIALALAADPELIIADEPTTALDVSIQAQIIQLLKRLCRERGTAVMLITHDMGV
                     IAETADRVAVMYAGRLVEIGPVRDVVHNPHHPYTKGLMGSIPTLDIDAPRLKQIPGSM
                     PRLNAIPKGCAFNPRCPEAIARCREQRPEAKVIDRSEVACWVAHHADAETDR"
     gene            2363..3373
                     /locus_tag="VFX46_07120"
     CDS             2363..3373
                     /locus_tag="VFX46_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5600388.1"
                     /translation="MSGPRREPPLVEVRGLSRTFDVSKPWLNRILEREPRRLLKAVQD
                     VSFTIGRRETFALVGESGSGKSTVAKMVVGLLEPTAGSVTIDGIDMWKGSKAGDLVAL
                     RRRIQMIFQDPYASLNPRWRVGRIIAEPIRAFGLAKDKAEIEHRTGELLRLVGLDPAD
                     ARKYPHEFSGGQRQRICIARALSSKPQFLVCDEPTSALDVSVQAQILNLMRDLQDQFG
                     LTYLFISHNLAVVRHMATRIGVMYLGRLVEVAPTAELFANPQHPYTRMLLDAVPDLKM
                     SGRPRKMIEGEIPNPIDPPPGCAFHPRCPFAVERCRKEVPLPVTRGDRMVACHEAAAR
                     RI"
     gene            3422..4213
                     /locus_tag="VFX46_07125"
     CDS             3422..4213
                     /locus_tag="VFX46_07125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5600389.1"
                     /translation="MSEKGVAVITGGSSGIGEATARRMAADGYAVAILDVNEEGGASV
                     VKAIEEAGGKATFLTCDVADANAVEAAAAKVEADIGPVINLVTAAGLIPNTESIMDMD
                     MAAHDRMWRVNYHGTLHACRSFGRRMIPRGRGSIVTLGSINSVLPLPLPAYNPGKAAV
                     ARLTQLLAVELGRHGIRVNSVAPTYVMTPPLRAKVEAGQRDLNKIMSVHALSSLPEPS
                     DIADAIAFLCSEQARRITGILLPVDSGWLAGVSYLTYAGGVPWQT"
BASE COUNT          772 a         1297 c         1351 g          892 t
ORIGIN      
        1 gcttggtctt ttccaaatga cgctcgtcac gcgcttggtt cggtcagaaa tgctcgaggt
       61 cttgcgcacg gactacatca agttcgcgcg cgcccgcggg ctcacgagcc gtgcaatcca
      121 tttcggtcat gcgttgaaga atacgttggt gccggtcatc accattatcg ggctgcaggt
      181 tggcgctttg atcgcgttcg cgattatcac cgagaccgtg tttcaatggc cggggatggg
      241 actcctcttc atccagtcgg tgcaaaatgc cgacattccc gtcatgtcgg cttacctcat
      301 gctggttgct ttcctgttcg ttctcatcaa tttcattgtg gacttgcttt acgtcgcaat
      361 cgatccgcgc attcgcatcg tgggaggagc gagctagagc aatggccgtg gaagcggagg
      421 gcacgaacgg caccgggaac cccggggaag agcagggtgt cgtagcgcgt attctcgaca
      481 gcgatctgct ccattcgttc ctgcgcaacc ggttggtggt ggttgcggcc atcgttacgc
      541 tggccttcgt cttggtgtct ctgctcgcgc cctggattgc gccgcatgat ccttacgacg
      601 tgcgcacgct gaacttgctc gactcccata ccccgcccgt ttgggaggag ggcggcgatc
      661 ccagatttct gctcggcacg gacgaccaag gccgcgatat tctctcgacg atattgtttg
      721 gagcgcgcag ctccattgcc attggcatct tatctgtcct gctggcggtc gcgatcggcg
      781 tgacgcttgg cctcattgcc ggatatttcg gcgggccgat cgacaccatc atcatgcgca
      841 ttgcagatgt gcagctgacg ttcccggcca tattgaccgc gttgctcatt gacggtgtgg
      901 cgcgcgtgct attcgggact cacgggagcg gtggaggcga gttctggatt ctcgtcgtct
      961 caatcgggct gtcgttctgg gtgcagtatg cgcgcacggt gcgcggcatc acgctggtcg
     1021 agcgcaacaa ggagtacgtt caggccgcaa gattgatcgg cctcagctcc ttcaacatca
     1081 tgttcaacca ggtgctgccg aacgtgatga gtcccgtgct ggtcattgcg accatcaatc
     1141 tggcgctggc aatcctcacg gaggcgacgc tgtcgttcct cggtgttgga ttacccccga
     1201 ccgagccgtc gctgggcaca ttgatccgca tcggcaacaa cttcctcatg tcgggtgagt
     1261 attggatcgt catcttccct ggcattgcgc tcgccgcact ggtgctggcc gtgaacttgc
     1321 tcggcgactg gctgcgtgat gcattgaacc ccaagctgcg atgagcccgg ctgttgtcga
     1381 tgagcacgcc cgttctttct gttcgacact tgcgggttga atttccgacg cggaagggcg
     1441 tgcttgtcgc cgtcgatgac gtttccttcg acatcgccga aggggaggtc ctgggcgtgg
     1501 tcggcgagtc aggcgcagga aagtcgatga cgggcagcgc ggtgatcggc ttgatcgagc
     1561 cgcctggccg gatcgcagga ggcgaggtgt ggctcaaggg ccagcggatc gacaatctac
     1621 cctacgagga catgcgcaga atccgcggcc gccggatcgg catgatcttc caggacccgc
     1681 tgacgagttt gaacccgctc tacaggatcg gagatcagct tatcgaaacc atccgcacac
     1741 atttgccggt cagcgaggga gaagcacgca aacgcgcaat caagctgctg accgatgtcg
     1801 gcattcaagg cgcagaggcg cgtatcaacg cctatccgca ccagttctcg ggagggatgc
     1861 gtcagcgggt ggtcatcgct ctggcgctgg cggcggaccc ggagctgatc attgccgacg
     1921 agccgacaac ggcgctcgat gtatcgatcc aggcccagat catccagctc ctgaaacggc
     1981 tttgccgcga gcgtggcacc gcggtcatgc tcatcactca cgatatgggc gtcatcgccg
     2041 aaacggcgga ccgggtcgcg gtgatgtatg cgggccgcct cgtcgagatt gggccagtcc
     2101 gcgatgtcgt gcacaacccg catcatcctt acaccaaggg gctgatgggc tcgattccga
     2161 cgttggatat cgatgcaccg cgcctgaagc aaattcccgg ctcaatgccg cggctcaatg
     2221 ccattccgaa gggctgcgcc ttcaatcccc gctgccctga ggcgattgcg cgctgccgtg
     2281 agcagcgccc tgaggcgaag gtcatcgatc gctcagaggt cgcttgttgg gttgcccatc
     2341 acgccgatgc ggagacagat cgatgagcgg gccccgtcgc gagccaccgc tcgttgaagt
     2401 tcgcgggttg tcgcgcacgt tcgatgtctc gaagccgtgg ctcaatcgca ttcttgagcg
     2461 cgagccgcgc agactgctga aggccgtcca ggatgtttcc ttcaccatcg gtcggcgcga
     2521 gacattcgcg ctcgtcggag agtcgggttc cgggaagtcg accgtggcga aaatggtcgt
     2581 ggggcttctg gagccgacgg cgggcagcgt caccattgat ggcatcgaca tgtggaaggg
     2641 atcaaaagcc ggtgacttgg tggcgctgcg ccgccgcatc cagatgatct ttcaggatcc
     2701 ttatgcgagc ctgaatcccc gttggcgcgt ggggcgcatc atcgccgaac ccatccgggc
     2761 tttcgggctt gccaaagaca aagccgaaat cgagcaccgc accggcgagc tgctgcggct
     2821 cgtgggcctt gatccggcgg acgcgcgaaa atatccgcat gaattctccg gaggccagcg
     2881 gcagcgaatc tgcattgcgc gcgccttatc gtcgaaaccg cagtttctcg tctgcgacga
     2941 gccgacatct gcactcgacg tctccgtgca ggcccaaatt ctgaacctca tgcgcgacct
     3001 gcaggatcag ttcggcctca cctacctttt catcagccac aacctggccg tcgtccggca
     3061 catggccaca cgtattggtg ttatgtatct tggccggctt gtcgaagtgg cgcctaccgc
     3121 cgagttgttc gctaatcccc agcatcctta cacgcgcatg ctgcttgatg ctgtacccga
     3181 tctgaaaatg agcgggcgcc cgcgcaaaat gatcgaaggc gagatcccaa atccgattga
     3241 tccgccgccg gggtgtgctt tccatccgcg ctgcccgttc gccgtggagc ggtgcagaaa
     3301 ggaagttccg ctgccggtta cgcgcgggga ccgcatggtg gcttgccatg aggcagccgc
     3361 tcgccggata tgagctgatc aacaaaacgc aacctgaagt ccgtccgcga ggaggcgcag
     3421 aatgtctgag aagggtgtag cagtaattac tgggggctct agcggtatcg gcgaagcaac
     3481 ggcacgccga atggctgcag acggttatgc cgtcgcgatt ctggatgtga acgaggaggg
     3541 cggcgcttcc gtcgtcaagg cgatcgagga ggctggcggc aaggcgactt tcctcacctg
     3601 cgatgtcgca gatgcgaacg ctgtcgaggc tgcggctgcg aaagtcgagg cggatattgg
     3661 cccggtgatc aacctcgtga cggcggccgg cctgatcccg aacaccgagt ccatcatgga
     3721 catggacatg gccgcccacg accgtatgtg gcgggtgaac tatcacggca cccttcacgc
     3781 ctgccggtcc tttggccgcc gcatgatccc aaggggccgc ggctctatcg tcacactcgg
     3841 ctcgatcaac agcgttctgc cgttgccgct gccggcctac aatcctggca aggccgccgt
     3901 cgcgcggttg acgcaacttc tggctgtaga gcttggccgg cacggcattc gggtcaacag
     3961 tgttgcgccg acttacgtca tgacaccgcc gctgcgcgcg aaggtggagg ctgggcagcg
     4021 tgacctcaac aagatcatga gcgttcacgc cctgagctct ttgccggagc cgagcgacat
     4081 cgccgacgcc attgcgttcc tgtgctcgga gcaggcgcgc aggattacag gcattctgct
     4141 ccctgtcgat tcagggtggc tcgcaggcgt gagctatctg acctatgccg gcggagtgcc
     4201 gtggcagact taatggggcc ccgccaatcc cgtagtggcg tcggccagca ccctctaaga
     4261 gagcccgcaa gaccttcgct gacggaaacg cacgcagggg tacggccgcg ct
//
LOCUS       DASUMG010000431         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_617548, whole genome shotgun sequence.
ACCESSION   DASUMG010000431 DASUMG010000000
VERSION     DASUMG010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617548"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /gene="tsf"
                     /locus_tag="VFX46_07130"
     CDS             <1..798
                     /gene="tsf"
                     /locus_tag="VFX46_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5600390.1"
                     /translation="KAAKKAGRVAAEGLIGVAVEGRKGAVVEVNSETDFVARNAQFQE
                     MVREISKLALQADGDLARLLAMPFPGKTVSVEDHVKEMIATIGENMGVRRTATLEAGA
                     DGVLVEYVHNRVAEGMGKIGVLVALESTGDKAKLAEFGRQIAMHVAAAAPLAVSAEDL
                     DPAVIERERKIYEEQAAASGKPADIVAKMAEGRLRKEFFQQVVLLQQTFVIDGKSTVE
                     QAVKEAERTVGAPIKITGFVRYALGEGIERKETDFAAEVAAAVSGTR"
     gene            1063..1683
                     /locus_tag="VFX46_07135"
     CDS             1063..1683
                     /locus_tag="VFX46_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX5600391.1"
                     /translation="MFTLYYAPNTCALATHIALLDAGAKYELKRVDFSKSEQQSPEYL
                     AINPKGRVPAMVTPRGILTETPALLVFVAQSFPEAKLAPLDDPFAFAKLQEFNSYLCS
                     TVHVAHAHRVRGYRWADDPAAIEAMKKKVPQSVGACFELIEREFLKGPWVMGDTYTVA
                     DPYLFTIAQWLEADGVDTSRLPRVMEHRARMMERPNVKRALEEERA"
     gene            complement(1709..2872)
                     /locus_tag="VFX46_07140"
     CDS             complement(1709..2872)
                     /locus_tag="VFX46_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016735505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5600392.1"
                     /translation="MPILLALSGAHLLNDLLQSMIPALYPLFKEAYLLDFAQIGLITL
                     AFQVTASLLQPVFGFITDKRPWPYAMVAGMGATLLGLLGLAFAGSYAMLVVSAAMVGL
                     GSAVFHPEATRMARHAAAGEHGFAQGVFQIGGQAGYAIGPLLAAMIVVPRGQVSLAWL
                     SMVALAAMVLMAWTGARYAVMRRSQVAAGKAQSGTTARPNLSTGRILLAMAVLMLLVL
                     SKNAYTAAFKSFYTFYLMEQFGVEVQLSQIMLFIFLGVGAIGVVIGGIIGDRIGRYRM
                     IWISILGALPFTLLLPFANLFWTGVLSAIISFIMSSAFAAILIYAIDLVPHRVGLVGG
                     LYYGLAFGLGGLAVAALGVLADKIGIIEVFKLCAWLPALGLLTILLPRMPNQR"
BASE COUNT          588 a          890 c          923 g          483 t
ORIGIN      
        1 aaggcagcca agaaggcggg ccgtgtcgcc gccgaggggc tgatcggcgt tgccgtcgag
       61 ggccggaagg gcgcggtggt cgaggtcaat tcggaaacgg acttcgtggc ccgaaacgcc
      121 cagttccagg agatggtgcg agagatttcg aagctcgccc tccaagccga tggcgacctt
      181 gcccgtctgc tggcgatgcc gtttcctggc aagacggttt ccgtcgaaga ccacgtgaag
      241 gagatgatcg ccaccatcgg cgagaacatg ggtgtgcgcc gcacggcgac gctcgaggcg
      301 ggtgctgacg gcgtgctcgt cgaatatgtg cacaaccgcg tcgctgaggg catgggcaag
      361 atcggtgtgc tggtggcgct ggagagcacc ggcgacaagg cgaagctcgc cgagtttggc
      421 cgccagattg cgatgcacgt cgctgcggcg gcaccactgg ccgtttccgc cgaggatctc
      481 gacccagcgg tgatcgagcg cgagcgcaag atctacgagg agcaggcggc tgcttccggt
      541 aagccggctg acatcgttgc caagatggcc gagggccggc tccgcaagga gttcttccag
      601 caggtcgtcc tcttgcagca gaccttcgtg atcgacggca agtccacggt cgagcaggct
      661 gtgaaggaag ccgaacgcac ggtgggcgcg ccgatcaaga tcactggctt cgtgcgctac
      721 gcgctcggtg agggcatcga gcggaaggaa acggactttg ctgctgaggt tgccgccgcc
      781 gtgagcggca cgcggtaagg cgctccttct gtcaagggac tagacctaac gatatttgaa
      841 accgcgcctc tattcggaca acggattagg ggcgcggttt tcattgatca gccgttcact
      901 gcgaattggc tgtccagcac gctccaatcg cagctctcca atccgcaggc tgtttggctt
      961 cgccaatcag ttcgaaattc cagccgcgcc atcttgtgct ccaccgacgg acaggtgaag
     1021 atcccgccaa gtcaggctgg tgatcaagtc agagcacaga cgatgttcac tctctattac
     1081 gcacctaata cctgcgcact cgccacccac attgccctgc tcgatgcggg agccaagtac
     1141 gagctgaagc gggtcgattt cagcaagtcg gagcagcagt cgcctgagta tctggcgatc
     1201 aatcccaaag ggcgagttcc ggccatggtg acgccgcgcg gcatcctcac cgagacgcct
     1261 gccctgctcg ttttcgtcgc ccagagcttt cccgaagcga agctcgctcc gctagacgat
     1321 ccatttgctt ttgcgaagct gcaggagttc aacagctatc tctgctcgac cgtccacgtc
     1381 gctcacgccc acagggtccg cggctatcgg tgggcagacg acccggcggc catcgaggcg
     1441 atgaagaaga aggtgcctca gtcggtcggc gcctgcttcg agctgatcga gcgcgagttc
     1501 ctgaaaggtc cttgggtcat gggcgacaca tacaccgtcg ccgatcctta cctgttcacg
     1561 atcgcccagt ggctggaggc cgacggggtc gacacatcgc ggctccctcg tgtcatggag
     1621 catcgcgccc gaatgatgga gcggccgaat gtgaaacggg cgcttgagga ggagcgcgcc
     1681 tgaaaaggcg agctccggcc cagcctcttc atcgctggtt cggcatccgc ggcaggagaa
     1741 tggtcagcag ccccaacgcc ggtagccagg cgcagagctt gaacacttcg atgatgccga
     1801 tcttgtcggc aagaacgccg agggctgcga ccgccaaacc gcccaggccg aaggccaggc
     1861 cgtaatacag cccaccaacc aatcctacgc ggtgcggaac gaggtcaatc gcgtagatga
     1921 ggatggcggc gaaggcgctc gacatgatga agctgatgat ggcgctgagc acgccggtcc
     1981 agaacaggtt tgcgaacggc aggagcagag tgaacggcag tgcgccgagg atcgagatcc
     2041 agatcatgcg atagcggccg atcctgtccc cgatgatgcc gccgattacg acgccgatcg
     2101 caccgacgcc aaggaagatg aacagcatga tctgagaaag ctgcacctct accccgaact
     2161 gctccatcag gtagaaggtg tagaaggact tgaaggctgc ggtgtaggcg ttcttcgaca
     2221 gcacgagcag catcagcacc gccatcgcca gcagtatgcg cccggtgctc aggttcggtc
     2281 ttgctgtggt gccggattgc gcttttccgg ccgcaacctg cgagcggcgc atcaccgcgt
     2341 agcgcgcgcc cgtccatgcc ataaggacca tcgccgcaag cgcgaccata gaaagccatg
     2401 cgaggctgac ctgcccgcgc ggaaccacga tcatcgccgc gagcagcggc ccgatcgcgt
     2461 aaccggcctg cccgccgatc tggaagacgc cctgcgcgaa gccgtgctcg ccggctgccg
     2521 catggcgcgc cattcgcgtc gcctccggat gaaagacggc cgatccgagc ccgaccatcg
     2581 cggcggaaac gaccagcatc gcgtaactgc cggcgaacgc cagccccagt agtcccagga
     2641 gcgtcgctcc catcccggcg accatcgcat aaggccacgg cctcttgtcg gtgatgaacc
     2701 cgaagacggg ctgcagcagg gaagcggtca cctggaaggc gagcgtgatc aatccaattt
     2761 gcgcgaaatc cagcaggtac gcctccttaa acagagggta cagcgccgga atcatcgatt
     2821 ggagcaggtc gttgagcaaa tgcgccccgc tcagtgccag cagaatcggc atgagcgggc
     2881 ggtg
//
LOCUS       DASUMG010000432         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_617855, whole genome shotgun sequence.
ACCESSION   DASUMG010000432 DASUMG010000000
VERSION     DASUMG010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617855"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..777)
                     /locus_tag="VFX46_07145"
     CDS             complement(94..777)
                     /locus_tag="VFX46_07145"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEX5600393.1"
                     /translation="MLDELDRLAEVAGLGVRQVFLTGHRMTSDRDIFDALDLANARSL
                     LRFDSLAARARIEQLPWIKQAAITRVFPDSINVEVTEREAFAVWHMPDGEVLIDATGR
                     VLGPAPASMRAKLPRLAGEGAARDPSAIVNILAGFPALQARVEEIVRVAKRRWTLRLA
                     GGTEIHLPAGRETEALNALMTNPAARHLLDGRVYAAIDLRSPDRIVIRPRHSEEQKTA
                     SRYGAQPSG"
     gene            complement(1130..2068)
                     /locus_tag="VFX46_07150"
     CDS             complement(1130..2068)
                     /locus_tag="VFX46_07150"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX5600394.1"
                     /translation="MSADSAGRKYEHVAVLMGGWSAEREVSLSSGNACAAALEAAGYR
                     VTKIDVDRDIARQLKEIAPDACFNALHGRWGEDGCIQGLLEVMGIPYTHSGVRASALA
                     MNKAQTKRIVAPLGVPVCEHRIVNRTEAARTHAMEPPYVAKPICEGSSVGVVIVREGA
                     NKPPESVASVPAINDEIMVERFVPGRELTCAVIGDRATDIIDIVPAERLAFYDYEAKY
                     APGGSRHILPADIPRDVYEAVRQHALTVHNALGCRGVTRSDFRYDPASGQLAFLEINT
                     QPGMTGTSLVPEMAEYAGMSFQQLVTWLIEDASCNR"
     gene            complement(2226..>2471)
                     /locus_tag="VFX46_07155"
     CDS             complement(2226..>2471)
                     /locus_tag="VFX46_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylenolpyruvoylglucosamine reductase"
                     /protein_id="HEX5600395.1"
                     /translation="SAWKLIDAAGCRGMRIGGAKVSEMHCNFLINEGDATAEDIERLG
                     ETVRARVKANSGVTLEWEIVRLGLPLDGRPTGEALVA"
BASE COUNT          459 a          789 c          787 g          436 t
ORIGIN      
        1 gaggcagcag atcttgctgg tgcctacatc gatgagccca aggacctcgc cggcggatgt
       61 gttgattggc atcggatcat ctcctgcgca gggtcatccc gagggctgag ctccgtagcg
      121 ggaggcggtc ttctgctcct cactgtgccg tgggcggata acgatgcggt caggtgaccg
      181 gaggtcgatg gcggcataga cgcggccatc caggagatga cgggcggccg gattcgtcat
      241 gagcgcattc agcgcctcag tttcgcgacc ggccggcagg tggatctcgg ttccgcctgc
      301 gagacgcaga gtccaccgac gcttggctac tcggacaatt tcctcaactc gggcctgcag
      361 cgccggaaat cccgcgagaa tgttgacgat tgcggaaggg tcgcgcgcag cgccctctcc
      421 agccagccga ggcagcttgg ctcgcatgct ggccggtgca ggccccagca cgcgtccggt
      481 agcgtcgatc agaacttctc cgtcgggcat gtgccacaca gcgaatgcct cgcgctccgt
      541 gacctcgaca ttgatgctgt cgggaaaaac gcgggtgatc gcggcctgct tgatccaagg
      601 cagctgctcg atgcgagcac gggcggcaag gctgtcgaag cgcagcagag agcgcgcgtt
      661 cgccagatcg agcgcgtcga agatgtcccg atcagacgtc attcggtgac cagtgaggaa
      721 cacctggcgc acaccgagcc cagcaacttc ggcaagtcgg tccaattcat cgagcactgg
      781 acgcacctgt cgcgcgttcg cgccccagtg cgtaagggcg gcaaaagcaa gcgcgcctgc
      841 gatggctgtg gtgaaggcgg tgagcctgaa gccgcgacgc cgcgaacgtg gccgagcccg
      901 ggcgctgcta agcacgccgg cggaggcagg cgtactcccg cgccgctgat gcgcggacgc
      961 ccatcgattg ctcggagctc gtgcggagtt gagcacctcg gactctccgt tcgcgacgca
     1021 agaaatgccg tgaagtcgag ccggcccccc gctaccggca ctccacactc cagcgtcggc
     1081 gaaagaggtt aatttttggt tgagcgctga ccgctaggtc attgagccgt caacggttgc
     1141 acgaggcatc ctcgatcagc caggtgacga gctgctgaaa gctcatgccg gcgtactcgg
     1201 ccatttcggg caccagcgac gtgcctgtca tgccgggttg ggtgttgatc tcgaggaagg
     1261 cgagctggcc cgaagccgga tcgtaacgga aatcggagcg cgtgaccccc cggcatccga
     1321 gcgcattgtg cacggtgagt gcgtgctgcc tcacggcttc atagacgtca cgaggaatat
     1381 cggcgggcaa gatgtgccgg gagccgccgg gggcgtactt ggcctcgtaa tcgtaaaacg
     1441 ccagccgctc ggctgggacg atgtcgatta tgtcggtggc gcgatcaccg ataacggcgc
     1501 aggtcagctc gcgtccagga acgaagcgct cgaccatgat ctcatcgttg atcgcgggca
     1561 cgctcgcgac ggactccggc ggcttgttag caccttcgcg tacgatgacc acgccgacgc
     1621 tcgagccctc gcaaatgggt tttgcgacgt aaggcggctc cattgcatgc gtgcgcgcag
     1681 cctcagtgcg gttcacgatc cgatgctcgc aaacgggcac gccgagcggg gcaacgatgc
     1741 gcttcgtctg cgccttgttc atggcgagcg cggatgcgcg gacgccggag tgggtataag
     1801 ggatgcccat gacctcgagg agaccctgta tgcatccgtc ctcgccccaa cggccatgga
     1861 gtgcgttgaa gcaggcgtca ggagcgatct ctttcagttg ccgggcgatg tcccggtcca
     1921 cgtcgatctt cgttacacga tagccggccg cctcgagcgc ggcagcgcag gcattgcccg
     1981 agctgaggct cacctcccgt tcggcggacc atccgcccat caggacggcg acgtgctcat
     2041 acttgcggcc tgcactatct gcactcatgg cttatatgcc ccagcactca acgtcccagc
     2101 tcgcctgcat ccacacacgt aatcccgggc agaaggcccg ggatccacct gaccgcaaat
     2161 gccggagcaa gcggaaaaac cggtgacaag caccgggatg acgtttcttt tcgaaagagc
     2221 gcggttcacg caaccaatgc ctcgcctgtc ggccggccgt ccagaggcag acccaaccgc
     2281 acgatctccc attcaagagt cacgccagaa ttcgccttca ctctggcccg caccgtctca
     2341 ccgaggcgtt cgatgtcctc cgccgtcgca tcgccttcgt tgatcaggaa gttgcagtgc
     2401 atctctgaaa ccttcgcgcc acctatgcgc atgccgcggc aaccagcagc gtcgatgagc
     2461 ttccaggcgc t
//
LOCUS       DASUMG010000433         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_377510, whole genome shotgun sequence.
ACCESSION   DASUMG010000433 DASUMG010000000
VERSION     DASUMG010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377510"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..885
                     /locus_tag="VFX46_07160"
     CDS             220..885
                     /locus_tag="VFX46_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX5600396.1"
                     /translation="MMHLFPNPLIIPIGLIVGVLVSAPVGPVNVLCIQRAIERGFWGG
                     VAAGAGAVLGDGLIALCAALGVGAISGAIEDYRNAIQFIGGLALVGFGIKLYVSLPRI
                     ITGSEAEKPASIIEFLWDTPKTFFLTITNPGAVLGLFAIFGGVSSFVEVQSTVDAITM
                     VAAIIAGSFAWWVLLSSLIGRIRHRFAFGRINQINRIAGVLLFGFGAILIGELVLQFA
                     GLP"
     gene            complement(911..1156)
                     /locus_tag="VFX46_07165"
     CDS             complement(911..1156)
                     /locus_tag="VFX46_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600397.1"
                     /translation="MQAVSEAEDQSDSFKALSLILEAWDEGAEQGIPSELMAYAALYT
                     ALTDLVSAFGEEAVANMTRSLDKRVRQGEFTIYGTTQ"
     gene            complement(1276..1680)
                     /locus_tag="VFX46_07170"
     CDS             complement(1276..1680)
                     /locus_tag="VFX46_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HEX5600398.1"
                     /translation="MGRPPIRACFALALPGLLVALVILYSAAGIAHAQDSKPYDDKLM
                     RLSEILGAVHYLRELCGADEGQLWRDRMRELIEAEGTTALRRAQLTRSFNKGYLGYSR
                     TYRTCTPSAETAVKRFLSEATDLATELVRNVP"
     gene            complement(1710..1976)
                     /locus_tag="VFX46_07175"
     CDS             complement(1710..1976)
                     /locus_tag="VFX46_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600399.1"
                     /translation="MEQLEESTRLIIEKYRRRFGDLPHLPAIELDAELRGRFVAALER
                     ALSRNMPLFDYELQEFEISRSRQLWLRLKRKIMRLTTHRASARS"
     gene            complement(2115..>2430)
                     /locus_tag="VFX46_07180"
     CDS             complement(2115..>2430)
                     /locus_tag="VFX46_07180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX5600400.1"
                     /translation="VRIGAQPGALLLGPLAVEPFLAGKGVGRRLIAESLEAAKAEGIR
                     LVVLVGDLPYYGRFGFFRIPPGQIRLSGPVDPDRLLAAELVPGALTSFSGLVKADTDA
                     SP"
BASE COUNT          463 a          730 c          733 g          504 t
ORIGIN      
        1 cacgtcagcg aaattaccaa tagacatccg cgaattagcg gggtcggggc gcgtaaccaa
       61 ctcatgaggg aggactccat tgcagctgca gcgcaggtgc atgcgcaagc ttctcagctt
      121 gaggacgcca tccgtcaatg ccgtgcgccg gcaggcgttg cctcacggct gtggtgattt
      181 cgcgaggagc actgccggat tgggcgccct tccgtttgaa tgatgcacct ttttcccaat
      241 ccgctgatta tccctatcgg tctcatcgtt ggagtgctcg tttccgcgcc ggtgggtccg
      301 gtgaacgtcc tgtgcatcca gcgcgcgatt gaacgcggtt tttggggcgg tgtggcggcg
      361 ggcgctggag ccgttctggg cgacggactg attgcccttt gcgcggcctt gggtgtcggg
      421 gccatttccg gagcgatcga ggactatcgc aacgcgatcc agttcatcgg cgggctggcg
      481 ctcgtcgggt tcggaataaa gctctacgtg tcgttgccgc gcattatcac tggaagtgaa
      541 gcggagaagc cggcatcgat catcgagttc ctttgggata cgccgaaaac cttctttctg
      601 acgatcacca atcctggagc tgtccttggc ctgtttgcca ttttcggcgg cgtctcgagc
      661 ttcgtcgagg tgcagtcgac cgtagatgcg atcactatgg tcgctgctat catcgccggc
      721 agcttcgcct ggtgggtcct gctttcctcg ctgatcggcc ggatacggca tcgcttcgcc
      781 ttcggccgaa tcaaccagat caaccggatc gcgggcgtct tgctattcgg atttggcgcc
      841 attctcatcg gcgagctggt gctccagttc gccggtctgc catgacaccg aaatctgccc
      901 ggagcagcgg ctactgcgtc gtgccgtaga tcgtgaactc gccttggcgc acgcgcttat
      961 cgaggctgcg cgtcatgttg gcaacggcct cctcgccaaa ggctgaaacg aggtccgtca
     1021 gggccgtata gagcgccgcg taggccatca actcggaggg gatgccctgc tcagcaccct
     1081 cgtcccaagc ttccagaatg aggctcagcg ccttgaagct gtcgctctgg tcttctgctt
     1141 cggagaccgc ttgcatattg ctcgtccttt gcacttgtgt gtgcattccc acaacgcaag
     1201 ccctagcaca tcgctcggct cgagtggctg caacaacgat taagagtaaa tctcgggaaa
     1261 gctggggagt tgccgtcagg gcacattacg caccagctcg gtcgcaaggt cggtcgcttc
     1321 ggaaaggaag cgcttgactg ccgtctcggc tgaaggtgtg caggtccggt acgtgcggct
     1381 gtaacccaag taccccttgt tgaagctgcg cgtcagttgc gcccgccgta gcgccgtcgt
     1441 gccttcggcc tcgatgagct cccgcatgcg gtcgcgccag agctggcctt cgtcggcacc
     1501 gcagagctcg cgcaggtagt gcacggcgcc gaggatctcc gagaggcgca tcagcttgtc
     1561 gtcatacggc ttgctgtcct gggcgtgcgc aattcccgct gcggaataca agatcaccag
     1621 cgcaaccaga aggccaggta gtgccaacgc gaagcaagct ctaattggtg gccttcccat
     1681 ttggcgtatt cctcggtggg acagggtggt catgatcgtg cagatgcgcg gtgggtagtt
     1741 aagcgcatta tctttcgctt caacctcagc cagagctgcc gcgaacgcga aatctcgaac
     1801 tcctgcagct cgtagtcgaa caaaggcata tttcggctca gtgcccgctc caaggcagcc
     1861 acgaatctgc cgcgcaattc cgcatcgagc tcgatcgccg gcaaatgagg caggtcgccg
     1921 aaacgccgcc gatacttctc gatgatgagg cgcgtcgatt cctcaagctg ctccatactg
     1981 ataacagcac ccggcccgca ttcgtcacta ccgaccgccg catctggtga atgctcaacg
     2041 tcagaataaa gaaaaatgga acgaaaatgc gggcggaggc ccgattgatg cgcttccaga
     2101 ctgagggtca gagattaagg acttgcatcc gtgtccgcct tcacgaggcc ggagaatgag
     2161 gtcagtgcgc cgggcaccag ctcagcggct aacaaacggt ctggatcgac cggtcccgac
     2221 aggcggattt gaccaggcgg gatacggaag aacccgaaac ggccatagta ggggagatca
     2281 ccgacaagca cgacgagccg aatgccttcg gctttcgctg cctccaggga ttccgcaatc
     2341 agccgcctac cgacgccctt tccagccagg aatggctcga ctgccagcgg accgagcagc
     2401 aacgctcccg gctgagcacc gattcggaca
//
LOCUS       DASUMG010000434         2582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_778797, whole genome shotgun sequence.
ACCESSION   DASUMG010000434 DASUMG010000000
VERSION     DASUMG010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778797"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1558)
                     /locus_tag="VFX46_07185"
     CDS             complement(<1..1558)
                     /locus_tag="VFX46_07185"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HEX5600401.1"
                     /translation="MTNEIPNQSPPFVDINLFTSDVALQEAVEREGADYALEQLTEFG
                     AVCGSAEAFERGRLANEYPPRLRTHDPKGRRLDIVEFHPAYHACMARSMAEGLHCSSW
                     ERDKTGNPLPAANVARAGAFYMAAQMEAGHCCPLTMTNASVPTLRLQPELAREWVPRI
                     LSRSYDPSFRPAAKKSSVTIGMGMTEKQGGTDVRANTTLAEPVANGGPGGEYILSGHK
                     WFFSAPMCDAFMVLAQAPGGLSCFLVPRHLPDGTVNALHLQRLKDKLGNRSNASAEVE
                     FHDALGWLIGEEGRGVPAIIEMVTYTRLDCAVASAGLMRLALAMAVHHARHRTVFGRL
                     LIDQPLMAHVLADMALDVEAAVALVFRLARSFDRIEDERAAAWRRLMTPVTKFWVCKI
                     APVMIGEAMECLGGNGYVEENGMARIYREAPVNSIWEGSGNVMALDVLRVLQREPDIA
                     QLVLQEIAQATSDDRRLEAALDRVASLLHEPRLLDVRARELVETLALTAAGAILRIHG
                     HPDVADAFIAT"
     gene            complement(1666..2145)
                     /gene="ruvX"
                     /locus_tag="VFX46_07190"
     CDS             complement(1666..2145)
                     /gene="ruvX"
                     /locus_tag="VFX46_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX5600402.1"
                     /translation="MTEPADFAARLAKGKRLLGLDLGTKTIGVALSDVTRTIASPAET
                     IARTKFKADAARLIQLAAEQEVGGLVLGLPRNLDGSEGPRAQATRAFARNFNRVSPLP
                     ILLWDERWSTMAAERMLIDADRSRKRRGELIDKVAAAIILQSALDRLKAFEASSVKE"
     gene            2271..>2582
                     /locus_tag="VFX46_07195"
     CDS             2271..>2582
                     /locus_tag="VFX46_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5600403.1"
                     /translation="MKITWFGHSCFRIETGNSVILIDPFLKGNPTFEKSGVSWDEATK
                     GVTHVALTHGHDDHVGDAAEICKSRDATLFAVYELAMHIGNQGAQKLEPMNTGGTVRT
                     AD"
BASE COUNT          488 a          818 c          803 g          473 t
ORIGIN      
        1 gcgtcgcgat gaaggcatcg gccacatccg gatggccgtg gatacgcagg attgcaccgg
       61 cggcggttag cgcgagcgtc tcgacgagct ctcgagcacg aacatcgagc agtctgggct
      121 cgtgaagcag acttgcgacc ctgtcgagtg cagcctccag ccggcgatcg tcggaggtgg
      181 cctgggcgat ttcctgaagg accaattgcg cgatatccgg ctcgcgctgc aggacgcgca
      241 gcacgtccag ggccatgacg ttgcccgagc cctcccaaat gctgttgacc ggggcctccc
      301 gatagatgcg cgccatgcca ttttcctcga cgtatccatt gccgccgagg cattccatgg
      361 cctcgccgat catcaccggc gcgatcttgc agacccagaa tttcgtcacc ggcgtcataa
      421 ggcggcgcca ggcggccgcc cgttcatcct cgatgcggtc gaagctgcgg gcgagccgga
      481 aaacaagcgc aactgccgcc tccacatcga gggccatgtc agccaacaca tgggccatga
      541 gcggctgatc gatgagcaaa cggccaaaga cggtccgatg tcgtgcatgg tggacggcca
      601 tggcgagcgc cagccgcatc aggcccgccg aggcgacggc gcaatccagc cgtgtgtagg
      661 tgaccatctc gatgatggcc ggcacgcccc gcccttcctc gccgatgagc cagcccagcg
      721 cgtcgtggaa ttcgacttcc gctgaagcat tcgaccggtt gccgagcttg tccttcagcc
      781 gctgcagatg cagcgcattc accgtgccgt cgggcagatg tcgcgggacc agaaaacagg
      841 agagcccgcc gggcgcctgc gccagaacca tgaaggcatc acacatcggc gccgagaaaa
      901 accacttgtg gccggaaagg atgtactccc cgccagggcc gccattggcg accggctcgg
      961 cgagcgtagt gttggcgcgc acatccgttc ctccctgttt ctcggtcatg cccatcccga
     1021 tcgtgaccga gctcttcttg gcggcaggcc ggaacgacgg gtcgtagctc cgcgacagga
     1081 tgcgcggcac ccattccctt gccagctctg gctgaaggcg aagggttgga acggaggcat
     1141 tggtcatggt cagcgggcag cagtggcccg cctccatctg cgccgccatg tagaatgcgc
     1201 ctgctcgggc aacgttagcg gccgggagcg gattgccggt cttgtcacgc tcccaagagg
     1261 agcagtggag tccttccgcc atgctccgcg ccatgcaggc gtgataggcg ggatgaaact
     1321 cgacaatgtc gagacggcgg cctttcggat cgtgtgtcct gaggcgcggc ggatactcgt
     1381 tcgccaaacg tccgcgctcg aaggcttcgg cactcccgca caccgccccg aactcggtca
     1441 gctgctcgag ggcatagtcc gcgccttccc gctccaccgc ctcttgcagt gcaacgtcgg
     1501 aggtgaacag attgatgtcc acgaagggcg gtgactggtt gggaatctcg tttgtcatgg
     1561 caatggtctg ccctggagct gcttcgcgat caggaggcgg tttgaatcac ttaatggtta
     1621 cgcgacgctg ggacagactc tcactacacg tcgagattgc aaagctcatt cctttacaga
     1681 ggacgcttca aatgccttca accgatccag cgccgactgc aggataatgg ctgcggccac
     1741 tttgtcgatc agctcacccc tacgcttgcg gctgcgatct gcatcgatca gcatgcgctc
     1801 ggcggccatg gtcgaccagc gctcgtccca tagcaggatt ggcaacggcg aaacgcggtt
     1861 gaaattccgg gcgaacgcgc gcgtggcctg ggcccgcggc ccctcgcttc cgtcgagatt
     1921 acggggaagg ccgaggacga ggccgcctac ttcctgctcc gctgcgagct ggatgaggcg
     1981 agctgcgtcg gccttgaact tggtacgggc aatcgtctcg gctggcgatg cgatggttcg
     2041 ggtgacgtcc gacagcgcta ctccgatcgt ttttgttccg aggtcgagac ccagcaggcg
     2101 cttacccttg gcgagccgcg cggcaaagtc cgcgggctct gtcattgtct cgccgagaga
     2161 gttctcagcc acaagctttg ctcacatgtg attgaaaggg ttgttgcatc cgaagcccga
     2221 cctatactgc ccccgaccgg taacgtcgct tgtgataaag ggaaatcctc atgaagatta
     2281 cctggttcgg ccactcctgc ttccgaattg agaccggcaa cagcgttatc ctcatcgatc
     2341 ccttcctcaa ggggaacccg acgttcgaga aatcgggcgt gtcctgggat gaggcgacga
     2401 aaggagtgac ccacgtggcc ctcacccacg gccacgatga ccacgtcggt gacgcagcgg
     2461 aaatctgcaa atcgcgcgat gcgacccttt tcgcagttta cgagctggca atgcacatcg
     2521 gcaaccaggg ggcgcaaaag ctcgagccca tgaacaccgg ggggaccgtg cgtaccgccg
     2581 ac
//
LOCUS       DASUMG010000435         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_698694, whole genome shotgun sequence.
ACCESSION   DASUMG010000435 DASUMG010000000
VERSION     DASUMG010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698694"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VFX46_07200"
     CDS             complement(<1..311)
                     /locus_tag="VFX46_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEX5600404.1"
                     /translation="MDEMAKTKLVELTAEIISAYVSNNEVVASELPALIQDIHQALSR
                     VTNNLPAPEREELRPAIPIKRSVTPDYIICLEDGKKFKSLKRHLRTHYNLTPEEYREK
                     WG"
     gene            855..1286
                     /gene="msrB"
                     /locus_tag="VFX46_07205"
     CDS             855..1286
                     /gene="msrB"
                     /locus_tag="VFX46_07205"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX5600405.1"
                     /translation="MPDETPPRRKPEIVKSEAEWRRQLTPEQFYVTRQDGTERAFTHP
                     YYSEKRPGTYHCVCCGAPLFSSRDKFDPGTGWPSFTQPIIQDAIIEHEDRALFMRRVD
                     VRCARCDAHLGYVYSDGPEPTGLRYSLNGVALQLRPEEGHQ"
     gene            1378..>3077
                     /locus_tag="VFX46_07210"
     CDS             1378..>3077
                     /locus_tag="VFX46_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX5600406.1"
                     /translation="MDAAARNPSKPTDAATHVTPPKAKKQPVKSVWHGVELVDEYAWM
                     RAENWQEVMRDPSLLPAEIRAHLEAENAYTEAMLADTRELQEQLFQEMKGRIKEDDSS
                     VPAPDGPWEYYTRYLTGAQYPLVCRRPRGGGPEQILLDGNKEAEGKTYWQLGGTARSP
                     DHKLLAYAVDDRGSELYTIRVRDLETGADLPDVIINTRGGMAWANDNRSLFYVRLDDH
                     QRPRFVGRHLIGTPSEQDTIVYHEPDAGFYVGLGYTQSRKFIVIDAHDHQTNECYLID
                     TDAPDEPMRLISARQTGHEYGVDHHEDQLIITTNSGGAEDFRICTAPLSAPGMENWRD
                     LVPHKPGRLILETVAFKNHLVRLEREDGLPRIVIRRFSDGAEHAIAFDEEAYSLGISP
                     GYEYDTTTLRFTYSSMTTPAQVFDYDMETRDRVLRKTQEVPSGHNPEDYITKRLYAPA
                     PDGETVPISLLYRKGTPIDGSAPLFLYGYGSYGISIPASFSISRLSLVDRGFIYAIAH
                     IRGGKEKGYRWYKQGKHKTKRNTFTDFIAAAEYLVSQGYTRRGRIIANGGSAGGMLVG
                     A"
BASE COUNT          657 a          887 c          913 g          620 t
ORIGIN      
        1 ccccacttct cgcgatactc ctcgggagtg agattgtaat gcgtgcgcag gtggcgtttg
       61 agggatttga acttctttcc gtcctcgaga cagatgatgt agtcgggcgt cacagagcgc
      121 ttgatcggaa ttgcgggccg cagctcctcg cgctcgggtg cgggcaagtt attcgttacg
      181 cgactgagag cctgatgaat atcttggatg agagccggta gctccgaagc gacgacctcg
      241 ttgttactta cataggcaga gatgatctca gccgtcagct cgacgagctt ggttttcgcc
      301 atctcgtcca tcttcttctc catcagtcac aacccccgga cgtgtttttc gagagcagaa
      361 caaccggccg cgaaggcaat gctctcagta ctgtgctagt ttatgcgtcc caacaacctg
      421 cgtgagcagt gcaggttgag tttatggaca acagatgggt tgggatgtca atactgactc
      481 aagctcaacc aaaagctttt gccaactaaa tcaggattag ttgcacagac gtatttggcg
      541 agatgtgaga ttttactgtc gctgcagttg caagtgtgcc acgagtctca tgacttgttt
      601 gcagagacag tcttcaaagc tccaagaggg gtgatgcgta tgtctagcaa gggtcctgac
      661 tcgggccaaa acatggcaaa cccgccggag agagggcgag cgctcgtgtg gcgcaccgtc
      721 tggaatgaaa aggatggggg gagcagttgg attgggtggc ggctcccatc cagccgatcg
      781 gcttcatata tgaatgctag gcgcattccg caccgagcca tgcttgccgg ctaaatatga
      841 caggaggtcc cgtgatgccg gatgagacgc cgccgcgccg caagccggag atcgttaagt
      901 ccgaggccga gtggcggcgg cagctgacgc ccgagcagtt ctacgtaacc cggcaggacg
      961 gcaccgagcg cgcctttacc catccctatt acagcgaaaa gcgacccgga acgtaccatt
     1021 gcgtctgttg tggtgccccg ctgttttcat cgcgcgacaa gttcgatcct ggaacaggct
     1081 ggccaagctt cacacagcca ataattcagg atgcaatcat cgagcatgag gatcgcgcgc
     1141 tgttcatgcg ccgggtcgac gtgcgatgcg ctcgctgtga cgctcatctt ggctacgtct
     1201 actccgacgg gccggagccg accgggcttc gctattccct caacggcgtg gcgctccaac
     1261 tcaggccgga ggaggggcat caatagcttg cgcgaacgac aaatgtcatt cctggtcgaa
     1321 acaaagtaat tgccagacct ttgagctgat ctggtgaaca atccggagaa gtttttcatg
     1381 gatgctgccg ctcgcaatcc ctccaagccg accgacgccg caacgcacgt aactccgccg
     1441 aaggccaaga agcagcccgt caagtctgtc tggcacgggg ttgagctggt tgacgaatat
     1501 gcctggatgc gcgccgagaa ctggcaggag gtaatgcgcg atccttcgct ccttccagcc
     1561 gagatccggg cacatctcga ggccgagaac gcctacacgg aggcgatgct cgcggataca
     1621 cgagagctcc aggaacagct cttccaggaa atgaaaggcc gcatcaagga ggacgacagc
     1681 tctgtgccgg ctcccgatgg tccttgggag tactacacgc gctatctgac tggtgcacag
     1741 tatccgctgg tgtgccggcg gccccgcggc ggcgggcctg agcaaatcct gctcgacggc
     1801 aacaaggaag cggagggaaa gacctactgg cagctcggtg ggacggcgcg cagccccgac
     1861 cacaagctcc tggcttatgc tgtcgacgac cgagggtccg aactctacac cattcgcgtg
     1921 cgagatctcg aaacgggcgc ggatcttccc gatgtcatca tcaatactcg gggtggaatg
     1981 gcgtgggcga acgataatcg ctccttgttc tacgttcgac tcgacgacca ccagcgcccg
     2041 cggttcgtcg gccggcatct catcggcact ccgagcgagc aggacaccat cgtgtatcac
     2101 gagcccgacg ccggctttta cgtgggtttg ggctacacgc agtcacgcaa gttcatcgtc
     2161 atcgatgctc acgatcatca gacgaacgaa tgttatctaa tcgataccga tgcgccggac
     2221 gagccaatgc ggctcatatc ggcgcggcag accggccacg agtacggcgt cgatcaccac
     2281 gaggaccagc tcatcatcac gacgaactcg ggcggtgcgg aggatttccg catctgcaca
     2341 gcgcccctct ctgcgcctgg catggagaat tggcgtgact tggtgcccca taaacctggc
     2401 cggctgatcc ttgagacggt cgccttcaaa aatcacctgg ttcgcctcga gcgtgaggat
     2461 gggcttccgc ggatcgtcat ccgacgcttt tccgatgggg ccgagcacgc catcgccttc
     2521 gacgaggaag cctactcgct cggcatttcg ccgggatacg agtacgacac gacgactctt
     2581 cgatttacgt attcctcgat gaccacgccg gcgcaggttt tcgactacga catggagact
     2641 cgcgaccgcg tgctgcggaa aactcaggag gtgccgagcg gccataatcc ggaggactac
     2701 atcacaaagc ggctctacgc gccggctccc gatggcgaaa cggtgccgat cagcctgctt
     2761 tatcgcaagg ggacgccgat tgacggttct gcaccgctgt ttctctacgg ctacggcagc
     2821 tacggaattt ccatcccggc gagcttctcg atttcacgat tgtcgctcgt ggacagaggc
     2881 ttcatttatg ccatcgcgca cattcgcggc ggcaaggaga aaggatatcg ctggtacaag
     2941 cagggcaagc ataaaacgaa gcgaaatacg ttcacggact tcatcgcggc tgccgagtat
     3001 cttgtgtctc aaggctatac ccgccgagga cggatcatcg cgaatggcgg atcggcgggc
     3061 ggcatgctgg tcggggc
//
LOCUS       DASUMG010000436         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_56766, whole genome shotgun sequence.
ACCESSION   DASUMG010000436 DASUMG010000000
VERSION     DASUMG010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56766"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2400)
                     /gene="gyrB"
                     /locus_tag="VFX46_07215"
     CDS             complement(<1..>2400)
                     /gene="gyrB"
                     /locus_tag="VFX46_07215"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX5600407.1"
                     /translation="PARKQNGAEHYGAENIKVLRGLDAVRKRPGMYIGDTDDGSGLHH
                     MVYEVVDNSIDEALAGFATEVQVILNADGSCTVIDNGRGIPVDIHPEEGISAAEVVMT
                     QLHAGGKFGSGGDENPYKVSGGLHGVGVSVVNALSSWLELTIWRDGKEHFVRFVNGVA
                     EAPLRVVGDAEGRRGTKVTFLPSPDTFKGVTEFSYETLEHRLRELAFLNSGVRVVLID
                     ERHADRKQEVMQYEGGLGAFVRYLDRAKHPLIETPIMIAAERDGVVVEAALWWNDSYH
                     ENMLCFTNNIPQRDGGTHLAGFRAALTRVINKYAEETGIAKKEKVSLTGDDAREGLTC
                     VLSVKVPDPKFSSQTKDKLVSSEVKPIVESVVGDQLAAWFEEHPKEAKAIITKVVEAA
                     AAREAARKARELTRRKGALDMANLPGKLAECQDRNPANTELFIVEGDSAGGSAKQARN
                     REFQAVLPIRGKILNVERARMDKMLSSQEIGTLITALGTGIGRGEDGGFDISKLRYHK
                     IIIMTDADVDGAHIRTLLLTFFYRQMPELIEKGHLYIAQPPLYKVTKGKSETYLKDQT
                     SLEDYLIDSGLTDAVLQLGNGESRAGQDLRDIVMQARTVQRLLDGLHSRYARFVVEQA
                     AIAGALDASLLNDREAAEAKAKEVAELLDNISEETERGWTGRFADNEGLVFSREVRGV
                     KEVHVLDRAFLTSLDARRLNERHEHLAEIYRLPAVLKRKDSETVITGPSSLLKAVYDA
                     GRKGITLQRYKGLGEMNADQLAETTLDPETRTLLQVKLGDLAEADEIFSKLMGDVVEP
                     RR"
BASE COUNT          415 a          767 c          736 g          482 t
ORIGIN      
        1 gcgccgcggc tcgacgacat cgcccatcag cttggagaag atctcgtccg cctcggcgag
       61 gtcgcccagc ttcacctgca gcagcgtgcg ggtctccggg tcgagtgtgg tctcggcaag
      121 ctgatccgcg ttcatctcgc ccagaccttt gtagcgctgc agggtaatgc ccttccggcc
      181 ggcgtcgtat acggctttga gcagcgatga cgggcccgtg atcaccgtct ccgaatcctt
      241 gcgtttcagc acggccggaa gccggtaaat ctcggcgagg tgttcgtgcc gctcgttcag
      301 gcggcgcgcg tcgagcgagg tcagaaaggc gcgatcaagc acgtgcactt ccttcacgcc
      361 gcggacctca cggctaaaga ccagcccttc gttgtccgcg aaccggcccg tccagccgcg
      421 ttccgtctcc tcggaaatgt tgtcgagcag ctcggcgacc tccttcgctt tggcctcggc
      481 cgcctctctg tcattgagca gcgaagcatc gagggcgcct gcaatggccg cctgctcgac
      541 aacgaaccgc gcgtagcgcg agtggaggcc atccagcagt ctctggaccg ttcgcgcctg
      601 catgacgatg tctcgaagat cctgccccgc gcgcgattcc ccgttgccga gctggagcac
      661 tgcatcggtc agaccgctgt cgatcaggta atcctcaagg ctcgtctgat ccttgaggta
      721 ggtctccgac ttgccctttg tgactttgta cagcggcggc tgggcaatgt agaggtgccc
      781 tttctcaata agctccggca tctgacggta gaagaacgtc aacagcagcg tacggatgtg
      841 cgcgccgtcg acgtcggcgt ccgtcataat gatgattttg tgataacgga gcttcgaaat
      901 atcgaagccg ccgtcctcgc cacggccgat gccggtgcca agtgcagtga tcagcgtgcc
      961 gatctcctgg ctggacagca tcttgtccat gcgcgcgcgc tcgacgttca ggatcttgcc
     1021 gcggatcggc aaaacggcct ggaactcgcg gttgcgcgcc tgcttggcgg agccgccggc
     1081 cgagtcaccc tcgacgatga acagctccgt gttggccgga ttgcgatcct gacattcggc
     1141 gagcttgccg ggcagattgg ccatgtcgag cgcgcccttg cgccgggtca gctcgcgggc
     1201 tttgcgcgcg gcctcgcggg cagcggcagc ctccaccacc ttggtgatga tggccttggc
     1261 ttctttcggg tgctcctcga accaggcggc aagctggtcg ccgacaacgc tctcgacgat
     1321 cggcttcacc tcggaggaga cgagcttgtc cttcgtctgg ctcgagaact ttggatcggg
     1381 caccttgacg gaaagcacgc aggtgaggcc ctcgcgggca tcatcgcccg tcaaggacac
     1441 cttctccttc ttcgcgatgc ctgtctcttc cgcgtatttg ttgatgacgc gcgtcagcgc
     1501 agcacggaag ccggcgaggt gcgtgccgcc atcccgctgg gggatgttgt tggtgaagca
     1561 gagcatgttc tcgtggtagc tgtcgttcca ccacagagcc gcttcaacca ccacaccgtc
     1621 gcgttcggcg gcgatcatga tgggagtttc gatgagcgga tgcttggcgc gatcgaggta
     1681 acgcacgaac gcgccgaggc cgccctcgta ctgcatcacc tcctgcttac gatcagcgtg
     1741 ccgctcatcg atgaggacaa cgcgtacacc ggagttcagg aaggcaagct cccggagccg
     1801 gtgctccagc gtttcatagc tgaattccgt tacgcctttg aaggtatcgg ggctcggcag
     1861 gaaggtgacc ttggtgccgc gacggccctc cgcatcgccg acgacgcgca gcggagcctc
     1921 cgccacgccg ttcacgaagc ggacaaagtg ctctttcccg tctcgccaaa tggtgagctc
     1981 gagccacgag gacagggcgt tgacgaccga aacaccgacg ccgtgcagac cgcctgaaac
     2041 cttgtacgga ttctcatccc cgccggagcc gaacttgccg ccagcgtgaa gctgggtcat
     2101 cacgacctca gcggccgaga tgccctcttc gggatgaata tcgacgggaa tgccgcggcc
     2161 attgtcgatg acagtgcacg atccgtcagc attcagaatg acctgaactt ccgtcgcaaa
     2221 gccggccagc gcctcgtcga tggagttgtc gaccacctca tagaccatgt ggtggaggcc
     2281 ggagccatcg tccgtatcgc cgatgtacat gccagggcgt ttgcggacgg catccaagcc
     2341 acgcaaaacc ttaatgtttt ccgcgccgta atgttcggcg ccgttttgtt tcctggccgg
//
LOCUS       DASUMG010000437         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_779171, whole genome shotgun sequence.
ACCESSION   DASUMG010000437 DASUMG010000000
VERSION     DASUMG010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779171"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VFX46_07220"
     CDS             complement(<1..175)
                     /locus_tag="VFX46_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor"
                     /protein_id="HEX5600408.1"
                     /translation="MDKRDDGADTRRFIRETGLAAEIAALVEPVLEDLGFRLVRVQVH
                     GRDGQTVQIMAERP"
     gene            complement(354..1088)
                     /gene="trmB"
                     /locus_tag="VFX46_07225"
     CDS             complement(354..1088)
                     /gene="trmB"
                     /locus_tag="VFX46_07225"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEX5600409.1"
                     /translation="MAEPEKAPIQPIRTFGRRHGRRLSSRQQRLLAEELPRLEINVSA
                     PPPRAATDLFPPGIRDVWLEIGFGGGEHLIWQAEQHPDVGIIGCEPYLDGVVKVVDAV
                     LTQGLSTVRVFPNDARNVLEWLPEASLGRVFILFPDPWPKTRHRKRRLVSAPFLRTLA
                     RVMKPGAELRIATDIGDYARTTLLAVRATPAFRWLASGPSDWRNRTADWPATRYEEKA
                     LAAGRRPIYLRFERLASNADPEILQK"
     gene            complement(1169..>2224)
                     /gene="metK"
                     /locus_tag="VFX46_07230"
     CDS             complement(1169..>2224)
                     /gene="metK"
                     /locus_tag="VFX46_07230"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX5600410.1"
                     /translation="VAAETLATTNHVVIAGETRGPDTITSDLVAHVARMAIRDIGYEQ
                     PGFHWETADVKVLLHPQSADIALGVDSSEEKDEGAGDQGIMFGYACRETPELMPAPIY
                     YSHRILQVLAAARKAREGDAVKLGPDSKSQVTVRYEDGKPVGVSQIVLSTQHLDPDLT
                     SNQVREIVEPYIRKTLPEGWIDSKTTWHVNPTGKFVIGGPDGDCGLTGRKIIVDTYGG
                     AAPHGGGAFSGKDPTKVDRSAAYAARYLAKNIVAAGLAERCSIQLSYAIGVAEPLSIY
                     VDTYGTGQVDEDKLEMAIRRVVTLTPRGIRKHLGLNKPIYARTAAYGHFGRQPDADGG
                     FSWEKLDLVDALKREVA"
BASE COUNT          400 a          694 c          700 g          430 t
ORIGIN      
        1 tcgggcgctc ggccataatc tggactgtct ggccgtctcg gccgtgaacc tgaaccctta
       61 ccaagcgaaa accgaggtct tccagaaccg gctcgacgag cgcggcgatt tctgcagcaa
      121 ggccagtttc gcgaatgaag cgccgggtat ccgcgccatc atcccgcttg tccatatcgc
      181 ctcgagttcc gcatccagct gctcatgagc agcggctaaa acaaaaagag cggacccgct
      241 ggggcccact ctcagagttg agatgatcat gagcgtttga aacatatgtg agttatgacg
      301 cgaaggtcaa gcgcaaatgg caaaaatcgg gcaacttgcc cggagatgaa ccctcatttt
      361 tgtaggattt cggggtccgc attggatgca aggcgctcga aacgcaaata gatcgggcgc
      421 cgccccgcag ctagggcctt ttcttcgtaa cgggtcgccg gccagtccgc cgtccggttc
      481 cgccagtcag acggcccgct cgccaaccag cggaaggccg gtgtggcacg gacggcgagc
      541 agagtggttc gtgcatagtc cccgatatca gttgcaattc ggagctccgc gccaggcttc
      601 atgacccgtg ccagcgtccg cagaaacggt gcggaaacca gacggcgctt gcgatggcgg
      661 gtcttgggcc aagggtccgg gaataggatg aacacgcgcc ctaacgaagc ctccggcagc
      721 cactccaaca cgttccgggc atcattcggg aacacgcgga cagtgctcag cccttgcgtc
      781 aagacggcat cgactacctt tacaactccg tcgaggtagg gctcgcagcc gatgatgccg
      841 acgtcagggt gttgttccgc ctgccagatg aggtgctcgc cacccccgaa tccgatctcg
      901 agccagacat cccgaatgcc gggcgggaac aggtccgttg cggcacgggg aggaggtgcg
      961 ctgacattga tctcgagccg cggcagctcc tcggcgagaa gccgttgctg gcgggagctc
     1021 agccggcggc cgtggcgccg cccgaaggtg cggataggct ggatcggggc cttctcaggt
     1081 tcagccattc cttaggtctg ctccttgaac gccaatcggc cgcggatgtg agccgcggcc
     1141 gattgcaaac agcacccgca ggtgcttctc aggcaacctc gcgcttgagg gcgtccacga
     1201 ggtcgagctt ttcccaagag aacccgccat cggcatccgg ctgcctgccg aagtgaccat
     1261 aggcagccgt ccgggcatag atgggcttgt tcaaaccgag gtgcttgcgg atgccgcggg
     1321 gcgtgagcgt gacaaccctg cggatcgcca tttccagctt gtcctcgtcg acttggccgg
     1381 tgccataggt gtctacgtaa atcgacagtg gctcggcgac gccgatggcg taggaaagct
     1441 ggatgctgca gcgctccgcc agccccgctg cgacgatatt cttcgcaagg taacgggcgg
     1501 cgtaggcggc cgaccggtcg accttggttg ggtctttgcc cgagaatgcg ccgccgccgt
     1561 gcggcgctgc gccgccataa gtatcgacga tgatcttgcg gccagtgagc ccgcagtcgc
     1621 catccggacc gccgatcacg aatttccctg tcgggttcac gtgccaagtg gtcttcgagt
     1681 cgatccaccc ttccggcaag gtcttgcgga tgtagggctc gacgatctcc cgcacctggt
     1741 tggaggtgag atctggatcg aggtgctgcg tcgagagcac gatttggcta accccgaccg
     1801 gcttcccgtc ctcgtagcgc actgtcacct ggctcttcga gtccgggccg agctttacgg
     1861 cgtcgccttc tctcgccttg cgggctgcgg cgagcacctg gaggatcctg tggctgtagt
     1921 agattggcgc cggcatcagc tccggggttt cccggcaagc gtagccgaac atgatgccct
     1981 ggtctccggc gccttcgtct ttctcttcgg agctgtcgac acccagagcg atatcagccg
     2041 attgtgggtg cagcagcacc ttcacatccg ccgtttccca gtgaaaccct ggttgctcgt
     2101 acccgatgtc acggatggcc atgcgggcca catgggcgac gagatcgctg gtgatcgtat
     2161 cagggccgcg cgtctctccc gcgataacga cgtggttcgt cgtcgccagt gtctcggcgg
     2221 ctac
//
LOCUS       DASUMG010000438         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_298238, whole genome shotgun sequence.
ACCESSION   DASUMG010000438 DASUMG010000000
VERSION     DASUMG010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298238"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="VFX46_07235"
     CDS             <1..983
                     /locus_tag="VFX46_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600411.1"
                     /translation="AVASPVRIMQLVSIARNPVPSGAVVGAFEGYDGNPMRFARWEPT
                     RGPTRGTVCVFTGRSEFIEKYFEVVSDLRRRGFAVAVMDWRGQGGSYRPLSNPRKGWV
                     RTFSEYDRDLDCFMEQVVKPECPPPYYALAHSMGGHILLRIAGRPVPPFERIMVVAPM
                     LRFHDKKVGVSQRYARLYAALGTACGFGRAYVRGGSDASEDPVVFEGNPLTSDEARFA
                     RNRALIEAAPHLLLGAPTIGWLNAAYRSMAKLNDPAYAERISVPLLVAIAGQDVIVDP
                     VASEAFAARVKLCTTVNLPLARHEILQEADEIRGRFWAAFDAYLGVEMVS"
     gene            complement(1028..1828)
                     /gene="hisN"
                     /locus_tag="VFX46_07240"
     CDS             complement(1028..1828)
                     /gene="hisN"
                     /locus_tag="VFX46_07240"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HEX5600412.1"
                     /translation="MSNKAGFEAFVATAHELADAAGAAILPHFRRRIEIENKASERRG
                     FDPVTAADRAAERAISKLLANRFPDHGLEGEEYGHRNPEARFRWVVDPIDGTRAFILG
                     MPIWGTLIGLLDGETPVMGMMDQPYTGERFWSGQRASYFRGRDGKERRIKTRTCASLA
                     DAMLTTTDPELFVSAKDKRAFRAVRERVRAVRYGGDCYTYCLLAAGLVDIIVETGLKP
                     HDVVALIPIIERAGGRITTWEGESAVRGGRIVATGDPRLHEQALSLLA"
BASE COUNT          449 a          848 c          815 g          571 t
ORIGIN      
        1 aggcggtcgc aagcccggtt cgcatcatgc aactcgtcag catcgcgaga aacccggtgc
       61 ccagcggcgc ggtcgttggc gcattcgaag gctacgatgg caacccgatg cgtttcgccc
      121 gctgggagcc gacacgcggc cccacgcgag gcaccgtatg tgtcttcacc ggccgcagtg
      181 agtttatcga aaagtacttc gaggtcgtct ccgatctgcg ccgccgcggt ttcgccgtcg
      241 ccgtcatgga ctggcgcggg cagggcggct cgtaccggcc attgagtaat ccccgcaaag
      301 gctgggtgcg gactttttcg gagtacgaca gggacctcga ttgcttcatg gagcaagtgg
      361 tcaagccgga gtgcccaccg ccctactacg cgctcgccca ttccatgggc ggacacatct
      421 tgctccgcat cgccggccgg ccggttccgc cgttcgagcg catcatggtg gtggcgccca
      481 tgctccgctt ccatgacaag aaggttggcg tatctcagcg ctatgcccgc ctgtatgccg
      541 cactggggac tgcgtgcgga tttgggcggg cctacgtgcg gggtggctcg gacgcgagcg
      601 aagatcccgt tgttttcgag ggcaatccgc tgacctcaga tgaagcccgg tttgcccgca
      661 atcgtgcgct gatcgaggct gcgccacatc tcctgctggg ggccccgacg atcggctggc
      721 tcaatgctgc ctacaggtcg atggccaagc tgaacgatcc cgcctacgcc gaacggattt
      781 ctgtgccgct gctggttgcc attgccggtc aagacgtcat tgtcgacccc gttgcttcgg
      841 aggccttcgc cgctcgtgtg aagctgtgca cgacggtcaa cttgccgctc gccaggcacg
      901 agatcctgca ggaggccgat gagatccgcg gccgcttctg ggccgcgttc gacgcctatc
      961 tgggcgtcga gatggtttcc tgatcccacc agagcgagct taggaagcgg ataaggcgct
     1021 gggtgaccta ggccagaaga gacagggctt gctcatggag gcgcgggtcc cctgtggcaa
     1081 cgatgcggcc gccgcgaacc gctgactcgc cctcccaggt cgtgatccgc cctccggcac
     1141 gttcgatgat gggaatgagc gcgaccacgt catgcggctt caggccggtc tcaacgatga
     1201 tgtcgacgag cccggcggca agcaggcagt aggtgtagca atcgccgccg taccgcacgg
     1261 cgcggaccct ctccctcacg gcccgaaaag cgcgcttgtc ttttgcggac acgaacagct
     1321 caggatcggt ggtggtgagc atcgcatcgg ccagtgacgc gcaggtcctg gtcttgatcc
     1381 gtcgctcctt gccgtcacgc ccccgaaaat agctcgccct ttgccccgac cagaaacgct
     1441 cgccggtgta gggttgatcc atcatgccca tcacgggggt ttcgccatcc aagaggccga
     1501 tgagcgtgcc ccagatcggc atgccgagaa tgaaggcgcg ggtgccatca atcggatcga
     1561 cgacccagcg gaaccgggcc tcgggattgc gatggccgta ctcctcgcct tcgaggccgt
     1621 gatcggggaa gcgattggcg agcagcttgc ttatagctcg ctccgccgca cggtcggccg
     1681 cggtgaccgg atcgaagccc ctcctctcgc tcgccttgtt ttcaatttcg atgcgccgac
     1741 ggaagtgtgg gagaatggcg gctcccgcag cgtcagcaag ctcgtgtgcc gtagcaacga
     1801 aggcctcgaa ccccgcctta ttgctcatat ccccccgcaa ttacgcttcc atggattgtt
     1861 cgcttgccta cccgccgagc cagcgatccg caatatcggg gcccgcttta agtgtgaacg
     1921 aattttcaca accggactta gatgtgttgc ttgattgcta ctaattaaac acttctggtc
     1981 aaactgctac agagctccct tgtaacgact catgaccatc tgcatacatc ctagatgatc
     2041 ttgcagcggt tttcctgcga gatcggtgcc caccttgggc gtttcctccc tagacttggc
     2101 cgttccaagc gagcggccac ttttttcttc atcgcacgcg ctctacttca gcgatgcagc
     2161 aggagatcta cctgcaggtc tttgctgctc tgcaaaaaat gcaattgtgg tttgtgcgct
     2221 gcatcgctat gtattgcggc gcggcagacg agcgcggctc gtttccgcag ccctccttgg
     2281 gcgtttcctc cctagacttg ggccgttcgg agacccggac ggcccctttt tcattcgcac
     2341 tacgggccgg cctcccgctg actccgccta gcgtgtcaga tattgcgacg tttcgtttcg
     2401 ccagcaggac ttgaaacggc gcatcctgca cctcatagta tgggcgtgag atcggtgctt
     2461 caccggtttc acagccctcc tttgggcgtt tcctccctag acttgggccg ttcggaaacc
     2521 cggacggcct ttttttctgt cgggagcagc tgcccggctg ctactcggca gcggcgcgcg
     2581 gtgcaatgat cgggggcagc tcggcgaaga tccgggccat catttgcgtg aggtcgcgac
     2641 ggagccggtt gaagttggct gttcgtgtca gcttctcctc gtt
//
LOCUS       DASUMG010000439         3467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_539405, whole genome shotgun sequence.
ACCESSION   DASUMG010000439 DASUMG010000000
VERSION     DASUMG010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3467
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539405"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /gene="gatB"
                     /locus_tag="VFX46_07245"
     CDS             complement(<1..1193)
                     /gene="gatB"
                     /locus_tag="VFX46_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX5600413.1"
                     /translation="MQDRAKAGKSSNLIPGATGDWEVVIGMEVHAQVNSRAKLFSGAS
                     TEFGGEPNSHVSLVDAAMPGMLPVINEECVAQAVRTGLGLKAKINLKSVFDRKNYFYP
                     DLPQGYQISQYKQPIVGEGEVEIDVDGETIKVGIERLHLEQDAGKSLHDQHPDYTYVD
                     LNRAGVALMEIVSKPDLRSARQAQAYVSKLRTIMRYLGTCDGDMEKGNLRADINVSVR
                     RPGEPFGTRCEIKNVNSIRFIGQAIEAEARRQIAILEDGGVIEQETRLFDPSKGETRS
                     MRTKEEAHDYRYFPDPDLLPLELTQEWVDALKADLPELPDEKKKRFMEQYGLSSYDAG
                     VLVAERAAADYFEKVAAGRDAKAAANWVINELFGRLNKEGLDIEQSPVSAEQLGRILD
                     LIASKT"
     gene            complement(1252..1569)
                     /locus_tag="VFX46_07250"
     CDS             complement(1252..1569)
                     /locus_tag="VFX46_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX5600414.1"
                     /translation="MSTRQPEECTDMSQVRAEIDRIDAALVDLIAERFGYVERAWQLK
                     LAAKQEANVPWRNQQVIDRVRARAAERGLPPDLCEALWRQMIGWFIQYEEEKLRAQLE
                     ARD"
     gene            complement(1585..3072)
                     /gene="gatA"
                     /locus_tag="VFX46_07255"
     CDS             complement(1585..3072)
                     /gene="gatA"
                     /locus_tag="VFX46_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEX5600415.1"
                     /translation="MSELTKLTLAEAREGLKSKSFTAKELTQAFIDAIEAGNPALNAY
                     VATTAERALAQAAESDARIARGEARPLEGLPLGVKDLFCTRGVHSQACSHILDGFEPT
                     YESTVTQNLWDAGAVMLGKLNMDEFAMGSSNETSYYGPCISPWRRNDGSNAKLVPGGS
                     SGGSAAAVAADLCLAATATDTGGSIRQPAAFTGTVGLKPTYGRCSRWGIIAFASSLDQ
                     AGPITKTVRDAAIMLRHMASHDPKDSTSVDAPVPDYEAEVGQSIKGLRVGVPKEYRVE
                     GMAAEIEELWQKGIAWLKDAGATIHEVSLPHTKYALPAYYIVAPAEASSNLARYDGVR
                     YGLRITGDDLIDTYERTRAEGFGKEVRRRVLIGTYVLSAGYYDAYYLKAQKVRALIKR
                     DFDEAWQKVDVLLTPTTPSPAFALGEKSGDPISMYLQDIFTVTVNMAGLPAISVPGGL
                     SAQGTPLGLQLIGKPFDEGTLLRVAQVIEDAAGRFPVPDRWWVKG"
     gene            complement(3224..>3467)
                     /gene="gatC"
                     /locus_tag="VFX46_07260"
     CDS             complement(3224..>3467)
                     /gene="gatC"
                     /locus_tag="VFX46_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002966038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX5600416.1"
                     /translation="RIKITDDEARELQAELSGILDWVDQLNEVDTSNVEPMTRVVPIR
                     LKMRKDEVTDGNIADDIVKNAPMTDDHFFVVPKVVE"
BASE COUNT          579 a         1107 c         1077 g          704 t
ORIGIN      
        1 gtcttggagg cgatgagatc gagaatacgg cccagctgct ctgcggagac gggcgattgc
       61 tcgatgtcga gaccttcctt gttcagccgt ccaaacagct cattgatgac ccagttggct
      121 gcggccttgg catcgcgccc ggctgccacc ttctcgaaat aatctgccgc cgcacgttcg
      181 gccacgagca caccggcgtc gtaggaggaa aggccgtact gctccatgaa gcgcttcttc
      241 ttctcgtcgg gcagctccgg caggtcggct ttcagcgcat ccacccattc ctgggtcagc
      301 tccagcggaa gcaggtcggg atcggggaag tagcggtaat cgtgcgcctc ctccttcgtg
      361 cgcatggagc gggtttcgcc cttgctcggg tcgaagaggc gcgtttcctg ctcgataacg
      421 ccgccatcct ccagaattgc gatctgccgg cgcgcttccg cctcgatcgc ctgaccgatg
      481 aagcgaatgg agttcacgtt cttgatttcg cagcgcgtgc cgaatggctc gccgggcctc
      541 cgcacggaga cgttgatgtc ggcgcgcagg ttgcccttct ccatgtcgcc gtcgcacgtg
      601 ccgagatagc gcatgatggt gcggagcttc gacacgtagg cctgcgcctg ccgggcggag
      661 cgcaagtcgg gcttcgacac gatctccatc agcgccacgc ccgcgcggtt gagatcgacg
      721 taggtgtaat ccgggtgctg atcgtgtagc gacttgccgg cgtcctgctc gagatgcagc
      781 cgctcgattc cgaccttgat cgtctcgccg tcgacgtcga tttcgacctc gccttctccg
      841 acgatgggct gcttgtattg tgagatctga tagccctggg gcagatccgg gtagaaatag
      901 ttcttccggt cgaagacgct cttgaggttg atcttcgcct tgagcccgag gcccgtgcgg
      961 acagcctgcg cgacgcactc ctcgttgatc accgggagca tgccgggcat ggcggcgtcg
     1021 accagagata cgtggctgtt cggctcgccg ccgaactctg tcgaggcgcc agagaacagc
     1081 ttggcccgcg agttcacctg cgcgtgcact tccatgccga tcacgacttc ccaatccccg
     1141 gtcgcgccgg ggatcagatt gctcgacttt cctgccttcg ctcgatcctg catggcttat
     1201 ccttgtgcta tcgaagcacg ttcgagcctc aaagagcgct gctctcgcgg cttagtccct
     1261 cgcctccaat tgggcccgca gcttctcttc ctcatactgg atgaaccagc caatcatctg
     1321 ccgccagagc gcctcgcaca gatccggcgg caaaccccgc tctgctgccc gtgcgcgcac
     1381 gcggtcgatg acctgctgat tgcgccacgg cacgttggct tcctgcttcg cggcgagctt
     1441 gagttgccag gcccgctcca cgtagccgaa cctttcggcg atgaggtcga cgagcgccgc
     1501 gtcgatacgg tctatttcag ctcgcacctg gctcatatcg gtgcactctt ctggttgacg
     1561 cgtgctcatc ggaatcctcc cccttcagcc tttcacccac catcgatcgg gcaccgggaa
     1621 gcgcccagcg gcatcctcaa ttacctgggc cacacgaagc agcgtgccct cgtcgaacgg
     1681 ctttccgatg agttgcaggc cgagcggcgt gccttgagcc gaaagaccgc ccggaaccga
     1741 aatggccggc agccccgcca tgttgaccgt gacggtgaaa atgtcctgca gatacatcga
     1801 gatcggatcg cctgatttct cgcccaaggc aaaggccggg gacggcgtgg tgggcgtcag
     1861 cagaacgtcg accttctgcc aggcctcgtc gaagtcgcgc ttgatcagag cgcgcacctt
     1921 ctgcgccttg aggtaatacg cgtcgtaata gccggcggag agcacgtaag tgccgatcaa
     1981 gacgcgccgg cgcacctcct tgccgaaccc ttccgcgcgg gtgcgctcgt aggtgtcgat
     2041 gaggtcgtcg cctgtgatcc tgaggccgta gcgcacgccg tcatagcggg cgaggttcga
     2101 tgacgcctcg gcaggggcga cgatgtagta ggccggcagc gcatatttcg tgtgcggcag
     2161 gctgacttca tggatcgtag cgccagcatc cttgagccag gcaatgccct tctgccagag
     2221 ctcctcgatc tcggcggcca tgccctcgac ccgatattcc ttgggaacgc cgacccgcag
     2281 acccttgatg ctttggccga cctcagcctc gtagtccggc acgggggcat cgacggatgt
     2341 cgagtccttg ggatcgtggc tggccatgtg cctcagcatg attgcggcgt cgcggacggt
     2401 cttggtgatg gggccagcct gatcgagcga tgacgcgaac gcgatgatgc cccagcgcga
     2461 gcagcgccca tacgtgggct tgaggccgac ggtgccggtg aacgccgcag gctgccgaat
     2521 ggagccgccc gtgtccgtcg ccgtggcggc aaggcacaga tcggcagcga cggcagccgc
     2581 cgagccgccc gacgagccgc cgggcaccag tttggcattg gagccgtcat tgcggcgcca
     2641 ggggctgatg cacgggccgt agtagctcgt ctcattggac gagcccatgg cgaactcgtc
     2701 catgttgagc ttgccgagca ttacggcgcc ggcgtcccag agattctggg tgacggtcga
     2761 ctcataagtc ggctcgaagc catcgagaat gtggctgcag gcctgggaat ggacgccgcg
     2821 ggtgcagaac agatccttca cgccgagcgg cagcccttcc agcggccggg cttcgccacg
     2881 ggcaatccgc gcgtcactct cggcagcctg cgccagcgcg cgctccgctg tggtggccac
     2941 atacgcgttg agagccgggt ttccggcctc tatggcatcg atgaaggcct gagtcagctc
     3001 ctttgcagtg aagcttttcg atttcaggcc ctcgcgtgcc tctgcgaggg tcaatttcgt
     3061 cagctcggac aatgtcgcct cgtcttggct ggtgggcgaa ggcggaaact atcgtcgccc
     3121 gtatgacctg ttacagaaga aagtggggca acgcgcaatc accccgaatt tcgtttctct
     3181 gcaatgcgct caaagcagtc atggtcgctc ggctcggtgg cgttcactcc accaccttcg
     3241 ggacgacgaa aaagtgatcg tcggtcattg gagcgttctt gacgatatca tcggcgatgt
     3301 tgccgtcggt aacctcatcc ttgcgcatct tgagacggat cggcacgacg cgcgtcatcg
     3361 gctcgacgtt ggaggtgtcg acctcgttca gctgatccac ccagtcgagg atgccggaga
     3421 gctccgcttg cagctcccgc gcctcgtcat cggtgatctt gatacgt
//
LOCUS       DASUMG010000440         2821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1181775, whole genome shotgun sequence.
ACCESSION   DASUMG010000440 DASUMG010000000
VERSION     DASUMG010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181775"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VFX46_07265"
     CDS             <1..1332
                     /locus_tag="VFX46_07265"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004690368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX5600417.1"
                     /translation="NGWYNGERTGSGTIVWLAREMLARGEIDHKQFVEMVASSAPSVG
                     HCNTMGTASTMNSLAEALGMSLPGCAAIPAPHRDRGQMAYLTGKRAVEIVLEDLRPSK
                     ILTRAAFENAIVVNSAIGGSTNAPIHINAIARHVGVELDNDDWERIGYDIPLLVNLQP
                     AGEYLGEDYYRAGGVPAVVAELIEQGKIREDAITVNGKTLVENCKGQFSKDRRVIKPY
                     SEPLKKQAGFLNLKGNLFDSAIMKTSVISEEFRQRYLSNPNDPNAFEGKAVVFDGPED
                     YHARIDDPSLEIDENTLLFIRGTGPIGYPGGAEVVNMRAPDYLLKKGVRALPCIGDGR
                     QSGTSGSPSILNASPEAANNGGLALLRTGDRVRIDLNKRTADILISDEELEQRRKELM
                     AAGGYKYPESQTPWQEIQRGLVDEQSRGAVLKNAVKYQRIAETKGIPRDNH"
     gene            1383..1787
                     /locus_tag="VFX46_07270"
     CDS             1383..1787
                     /locus_tag="VFX46_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5600418.1"
                     /translation="MRYEDPTAAVDWLCHAFGFQPHAVYKDDAGNVVHAELSFGNGMI
                     MLGPNIDSPFGRYMTMPKDAGGRCTQTVYVIVDDVDAHHSQANAAGAEIVMPLRDESY
                     GGREYSCRDPEGHIWTFGNYDPWAQPSSTAGV"
     gene            complement(1811..2635)
                     /locus_tag="VFX46_07275"
     CDS             complement(1811..2635)
                     /locus_tag="VFX46_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018115695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600419.1"
                     /translation="MPTISTRDGTQIYYKDWGTGQPVIFSHGWPLNSDAWESQMYAVA
                     SAGYRAIGHDRRGHGRSSQPWDGNDMDTYADDLAQLIEALDLRDVILIGHSTGGGEVA
                     RYIGRHGTDRVAKVVLVSAVPPLMLQTPENPGGLPISVFDDLRKATLADRSQLYKDLA
                     SGPFFGFNRPGAKVSQGAIDSFWLQGMMVGFKGAYDCIKAFSETDFTEDLKKFDVPTL
                     IIHGDDDQIVPIDAAGRASAKIVQNAELKVYPGGAHGLAVTAREQLTNDLLAFIKA"
BASE COUNT          548 a          846 c          901 g          526 t
ORIGIN      
        1 aacggctggt acaacggcga gcgcaccggc tccggcacca tcgtctggct ggcgcgcgag
       61 atgctggcca ggggcgagat cgatcacaag cagttcgtgg agatggtggc ctcctcggcg
      121 ccgtctgtcg gtcactgcaa cacgatgggc acggcttcga cgatgaattc tctcgccgag
      181 gcgttgggta tgagtttgcc gggctgtgcg gccatcccgg ccccccatcg cgatcgcggg
      241 cagatggcgt atctcaccgg caagcgggcg gtcgagatcg tgctcgaaga cttgaggccg
      301 tcgaagatcc tgacgcgcgc tgcgttcgag aacgccatcg tcgtcaactc cgccattggc
      361 ggttcgacca acgccccgat ccacatcaac gcgatcgcgc gccatgtcgg ggtcgagctc
      421 gacaacgacg actgggaacg gatcggttac gacatccctc tgctggtcaa cctgcagccg
      481 gcgggtgagt atctgggcga ggactattat cgcgcgggcg gtgtccccgc cgtcgtggcg
      541 gagctgatcg agcagggcaa gatccgcgaa gacgcgatca cggtgaacgg caagacgctg
      601 gtcgaaaact gcaaggggca gttctccaaa gaccggcggg tgatcaaacc ctatagcgag
      661 ccgctgaaga agcaggccgg gttcctcaat ctcaagggca acctgttcga cagcgccatc
      721 atgaagacct cggtcatcag cgaggaattc cggcagcgct acctttcgaa cccgaatgat
      781 cccaacgctt tcgagggcaa ggcggtcgtg ttcgatgggc cagaggacta tcacgcccgc
      841 atcgatgatc cgtcgctcga gatcgacgag aacacgctgc tgttcattcg cggcacgggt
      901 ccgatcggct atccgggtgg cgccgaggtg gtgaacatgc gcgcgcccga ttacctgctg
      961 aagaagggcg tgcgggcact gccgtgtatt ggcgacggac gtcagtccgg aacatcgggt
     1021 agcccctcga tcctcaatgc ctcgcctgag gcggccaaca acggcgggct ggcactgctg
     1081 cggacaggcg accgggtgcg catcgatctc aataagcgga ctgccgacat cctgatttca
     1141 gacgaggagc tggagcagcg ccgcaaggag ctgatggccg cgggtggcta caagtatccc
     1201 gagagccaga cgccctggca ggagatccag cgcggtctcg tcgatgagca aagccgtggc
     1261 gccgtgctca agaatgcggt caagtatcag cggatcgctg agacgaaggg cattccgcgc
     1321 gacaaccact gatccgaggc gtcatgggac cgaaggcgac aactgcgacg atgatctcgg
     1381 ccatgcggta cgaagatccg acggccgcgg tagattggct ctgtcacgcc ttcggtttcc
     1441 agccgcacgc cgtctacaag gacgacgccg gcaacgtggt tcatgccgag ctatcgtttg
     1501 ggaacggcat gatcatgctg ggtccgaaca tcgatagtcc tttcgggcgg tacatgacca
     1561 tgccgaagga cgcgggtggg cgctgtacgc agacggtcta cgtcatcgtc gacgatgtgg
     1621 acgcccacca ctcccaagcc aatgccgctg gggcagagat cgtcatgcct ctgagggacg
     1681 agtcctatgg cggccgggag tattcctgcc gcgatcccga agggcacatc tggaccttcg
     1741 gaaactatga tccgtgggcg cagccatcga gtacggcggg cgtttgagcc ggctcgaagt
     1801 cgcgcctgct tcaggccttg atgaacgcaa gcagatcgtt ggttagttgc tcgcgtgcgg
     1861 tgacggccag tccatgcgcg ccgccagggt agaccttgag ctcggcattc tgcacgatct
     1921 ttgccgaagc ccgcccagcc gcatcgatag gcacgatttg atcatcgtct ccgtggatga
     1981 tcagagtcgg cacatcgaac ttcttcaggt cttcggtgaa atcagtttct gagaaggcct
     2041 tgatgcaatc gtaggcgccc ttgaagccca ccatcatgcc ctgcagccag aagctgtcga
     2101 tcgccccttg cgagaccttg gcccctggtc ggttgaaccc aaagaatggc cccgacgcca
     2161 aatccttgta gagctgcgat cggtcagcga gcgtcgcctt gcgcaggtca tcgaagacgc
     2221 tgatcggaag accgcccggg ttttctggcg tctgcagcat gaggggtggg acggcgctga
     2281 cgagcacaac tttcgccacg cggtccgtgc catgtcgccc gatgtaacga gcaacttcgc
     2341 cgcctccggt cgagtgcccg atgagtatga cgtcgcgcag gtcaagcgcc tcgatcagtt
     2401 gggcaagatc gtcggcatag gtatccatgt cgttgccgtc ccacggctgg ctcgatcggc
     2461 cgtggccgcg gcgatcgtgc ccgatggcgc ggtagccagc ggaggcgacg gcatacatct
     2521 gcgattccca ggcatcggaa ttgagcggcc agccatgact aaagatcacc ggctgccctg
     2581 ttccccaatc cttgtagtag atctgcgtgc cgtctcgcgt tgagatcgta ggcatccgac
     2641 cgctcccact tgttttgctc tggggtcgcg gcggaaactt caactgtcgg gctgagttgc
     2701 ggtgcgcctc agctcgacag tcgaatttgg tcgaaaatcc ttaatcaggc tctgccctgg
     2761 attcagggca ggggctcgat acgatcggcc gatatattgg ccaaaaccgc tacgcttcag
     2821 g
//
LOCUS       DASUMG010000441         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_619177, whole genome shotgun sequence.
ACCESSION   DASUMG010000441 DASUMG010000000
VERSION     DASUMG010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619177"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="VFX46_07280"
     CDS             <1..461
                     /locus_tag="VFX46_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX5600420.1"
                     /translation="NLLAVIPGGSSMPLLPASMCDDLTMDFDSLQKLKSGLGTAAIIV
                     MDKSTDIVKAITRIAYFYKHESCGQCTPCREGTGWMWRVLDRMSRGEAQKHEIDLLFD
                     VTKQIEGHTICAFGDAAAWPVQGLIRHFRHEIEARIDRYQAANQQRVAAE"
     gene            467..2569
                     /gene="nuoG"
                     /locus_tag="VFX46_07285"
     CDS             467..2569
                     /gene="nuoG"
                     /locus_tag="VFX46_07285"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HEX5600421.1"
                     /translation="MPKQLIIDGQLIEVDDGLTLIQACEQAGIPIPRFCYHERLSIAG
                     NCRMCLVEVVGMPKPVASCAMGVSDLPPNKDGSPKEIRTNSPMIKKAREGVLEFLLIN
                     HPLDCPICDQGGECDLQDQTLIYGPGGSRFHENKRAVEEKYIGPLIRTFMNRCIHCTR
                     CVRFMTEVAGVEELGAIGRGEDMEITTYLQRGILSELSANVNDLCPVGALTHRPWSYM
                     ARPWELRHTQSIDVMDALGSAIRVDSRGREVMRVLPRINDDVNEEWISDKTRHVADGL
                     RTQRLDQPYARKNGRLEPVSWEEALRIAADRLRSARPERIGAIAGDLAAAEEMFALKD
                     LLRRLGVPNLDCRQYGEPLDPRLGRSSYIFNSTVVGIEEADVLLIVGSNPRIEAAVLN
                     ARILKRWRRGGMKVGLVGEKVDLTYPYEHLGTGPQTLAEIAAGRHSFMQALAEARRPM
                     VIVGSGAIARPDGAAVLSLAAQIALTAMRKSLPEWNAFNVLHAAAGRVGGLDLGFVPE
                     EGGRDLMGMLSGDMEVVYLLGADEIDTSALGDAFVIYQGTHGDAGAHRADLILPAAAY
                     TEKSGTYVNLEGRAQMTNRAVFPPGAAKEDWAIIRALSPLVGHQLPYDDIFALRAAMY
                     REAPSLARIGTVKPEGLEGVEALAQMGGGQPGTAPFRSIVKDFYLTNPIARASAIMAE
                     LSRLKALAESENTVEAAE"
     gene            2582..>2983
                     /locus_tag="VFX46_07290"
     CDS             2582..>2983
                     /locus_tag="VFX46_07290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX5600422.1"
                     /translation="MTASEIWWEYGWPLAIMLGQSLLLLVCLLILIAYLLLMDRKVWA
                     AVQLRRGPNVVGPFGLLQSFADLLKFVFKEPVIPSGAERTIFLLAPLATATFALSAWA
                     VIPVSEDGLGRWVISDINVGILYILAISSLGV"
BASE COUNT          524 a          909 c          996 g          554 t
ORIGIN      
        1 acaatctctt ggctgtcatt cccggcggtt cgtccatgcc gctcttgcca gcgtccatgt
       61 gcgatgacct gacgatggac ttcgactcgc tgcagaagct gaagtccggc ctcggcaccg
      121 ccgccatcat cgtgatggac aagtccaccg acatcgtgaa ggcgatcacc cgcatcgcgt
      181 acttctacaa gcacgagagc tgcggccagt gcacgccctg ccgcgaaggc acgggttgga
      241 tgtggcgtgt gctcgatcgc atgtcgcggg gcgaggcgca gaagcacgag atcgacctgc
      301 tattcgacgt caccaagcag atcgaaggcc acaccatctg cgcattcggc gatgcggcgg
      361 cttggcccgt gcagggcctg atccggcact tccggcatga gatagaagcc cgtatcgacc
      421 gctatcaggc ggcgaatcag cagcgggtcg cggcggagtg atatagatgc cgaaacaact
      481 catcatcgac gggcagctca tcgaggtcga tgacggcctc acgctgatcc aggcttgcga
      541 gcaggcgggc atccccatcc cacggttctg ctaccacgag cgcctgtcga tcgcgggcaa
      601 ctgccgcatg tgtcttgttg aggtcgtcgg catgccgaag cccgtcgcgt cctgcgcgat
      661 gggtgtgagt gatttgccgc cgaacaagga tggcagcccg aaggagatca ggaccaactc
      721 gccgatgatc aagaaggcgc gcgagggcgt cctggagttc ttgctcatca atcacccgct
      781 cgactgtccc atttgcgacc agggtggcga gtgcgacctg caggatcaga cgctcatcta
      841 cggaccgggc ggctcgcgct tccacgagaa caagcgagcg gtggaggaga agtacattgg
      901 gccgctgatc cgcaccttca tgaaccggtg cattcattgc acgcgctgcg tccggttcat
      961 gacggaagtg gcgggcgtcg aggagctcgg tgccatcggc cgcggcgagg acatggagat
     1021 caccacgtat ctccagaggg gtatcctttc ggagctgtcg gccaacgtga atgatctgtg
     1081 tcccgtcggc gccctgacgc accggccgtg gtcgtacatg gcgcgcccgt gggagctgcg
     1141 gcatacccag tcgatcgacg tgatggatgc cctgggctcc gcgattcgcg tggattctcg
     1201 cggccgtgag gtcatgcgcg tcctcccgcg catcaacgac gatgtgaacg aggagtggat
     1261 ctccgacaag acacggcacg tcgcggatgg actgcgcacg cagcgccttg accagcccta
     1321 tgcccgtaag aacggcaggc tcgagccggt gagctgggag gaggcactac gtattgcggc
     1381 cgatcggctg agatcggcgc ggccggagcg catcggagcc atcgcgggcg acctggcggc
     1441 tgccgaggag atgttcgcgc tcaaggacct cctgcgcagg ctgggtgtcc ccaacctgga
     1501 ttgccggcag tatggcgagc ctctcgatcc tcggcttggg cgcagcagct acatcttcaa
     1561 ttcgaccgtc gtgggaattg aagaagccga tgtcctcctg atcgtcggct ccaatccgcg
     1621 tatcgaggca gcggttctca acgcacgcat cctcaagcgg tggcgccgcg gcggcatgaa
     1681 ggttggcctt gtcggcgaga aggtcgatct gacctatccc tacgagcacc tcggaacggg
     1741 cccgcagacg ttggcagaga ttgctgcggg tcggcattcg ttcatgcagg ctctcgccga
     1801 agcccggcgg ccgatggtga tcgtcggcag cggagcgatc gctcgacccg acggcgccgc
     1861 agtgctgtcc ctcgcggcgc agatcgccct cacagcaatg cggaaatccc tgccggagtg
     1921 gaacgccttc aacgtgctgc acgcggcggc agggcgggtc ggcgggctcg acctgggctt
     1981 cgtgccggag gagggcggcc gtgatctcat gggcatgctg agcggtgaca tggaggtcgt
     2041 ttacctgctt ggcgccgacg agatcgacac gagcgccctc ggagacgcgt tcgtcatcta
     2101 ccagggcact cacggcgatg cgggcgcgca tcgagctgac ctcatcttgc cggcggcagc
     2161 ctacacggag aaaagcggca cctacgtgaa tctcgagggc cgcgctcaga tgaccaaccg
     2221 ggccgtcttc ccgcctggcg cggcgaaaga ggactgggcg attatccggg cgctgtcgcc
     2281 gctggtcggc caccaattgc cttacgacga catctttgcc ttgcgcgcgg cgatgtatcg
     2341 ggaagctccg tcgctggcgc gtatcggcac ggtgaagccg gaagggttgg aaggggtgga
     2401 ggcccttgcc cagatgggcg gagggcaacc gggcactgcg ccattccggt cgatcgtgaa
     2461 ggatttctat ctgacaaatc caatcgcccg cgcctctgcg atcatggcgg agctgagccg
     2521 gttgaaggcg ctcgccgagt cggagaacac ggtcgaggct gcggaatagg atggcaccaa
     2581 catgacggca tcggagatct ggtgggagta tggctggccg ctggcgatca tgctggggca
     2641 aagcttgttg ctgctggtct gcctgctgat cctcatcgcc taccttctgc tgatggaccg
     2701 gaaggtgtgg gccgcggtgc agctgcggcg tggcccaaac gtcgtcgggc cattcggtct
     2761 gctgcagtcg ttcgccgacc tgctgaaatt cgtcttcaag gagcccgtga ttccgtccgg
     2821 cgccgagcgg acgatctttc tgctcgcgcc gcttgcgaca gctactttcg cgctttcagc
     2881 ttgggccgtc attcctgttt cggaggatgg gctgggacgc tgggtgatct ccgacatcaa
     2941 cgtcgggatt ctatacatcc tggcgatctc ctccctcgga gtt
//
LOCUS       DASUMG010000442         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_860101, whole genome shotgun sequence.
ACCESSION   DASUMG010000442 DASUMG010000000
VERSION     DASUMG010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860101"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VFX46_07295"
     CDS             complement(<1..752)
                     /locus_tag="VFX46_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX5600423.1"
                     /translation="MTAQPTRLLSSIAPLAPAYDAWLCDVWGVMHNGVEAFASAVEAC
                     RNFRERGGIVLLLSNAPRPASAVAAQIDRYGVPRDAYDGIVTSGDLTRKLIIERAQAR
                     LFHLGPERDKPLFEGLNLNFVAPDEAELVICSGLLNDDVETPSDYTAMLEPLARRGVP
                     MICANPDLKVERGSRIVYCAGSLAAEYERLGGKVTYAGKPYGPIYDLAFERLAELKGR
                     PVPRERVLAIGDGVRTDIQGAADAGIDSVFIA"
     gene            complement(787..1821)
                     /locus_tag="VFX46_07300"
     CDS             complement(787..1821)
                     /locus_tag="VFX46_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX5600424.1"
                     /translation="MIRKFAEEISQPRSKSDEATTGEASEEKETVPALSQEDAISASV
                     GALRAVADEMRKPSEAALAAIEKGQLPPYRENPNAKGPLPPALRPEHTVMAAMAGSLA
                     AHKLDVFLEPIVSLADRKARHFEVSLRLRQGTADGLGPRDYIPMARKAGLLPVIDATC
                     IARAAMVARHMDERKTNGSLFAIVTADSLADERFLQEFGQAFRQVPSLRERLVMAITQ
                     SELGELTEGQWKTIRQLSENGFRFAVDEVTSLDLDLPDLQSSGFIFVRLPAQSMIDGL
                     AGLDGVVSGAEACKRLRDAKMTVVAVGLASEDQLTQLQAAGVTLGQGALFGVPRAIRA
                     EALQPAKSAA"
BASE COUNT          375 a          664 c          647 g          351 t
ORIGIN      
        1 ctggcgatga agaccgagtc gattccggcg tcagcggctc cctggatatc ggtgcgcacg
       61 ccgtcaccaa tcgcaagtac acgctcgcga ggaacgggcc tacccttcag ctcggcaagc
      121 ctttcgaagg ccaggtcgta aatggggccg tagggcttcc cagcataagt caccttgcca
      181 ccgagccgct cgtactctgc agcgagagaa ccagcgcagt agacgatgcg ggagccgcgc
      241 tcaaccttca gatcgggatt ggcgcagatc ataggcacgc cccgccgtgc taacggctcc
      301 agcatggccg tgtaatcgga gggggtctcg acgtcgtcgt tcagcaggcc cgagcagatg
      361 accagctcag cctcatcagg agcgacgaag ttcagattaa gcccctcgaa caacggcttg
      421 tcgcgctcgg gaccgagatg gaacaggcgc gcctgcgccc gttcaataat cagcttccgg
      481 gtaagatcgc cggacgtgac gatgccgtcg taggcatccc tgggaacgcc gtagcgatcg
      541 atttgcgcgg ctaccgccga agccggtcga ggggcgttcg acagcagcag cacaatgccg
      601 ccacgttcgc ggaaattgcg gcaggcctcg accgccgagg cgaacgcctc gacgccgttg
      661 tgcatcacac cccagacgtc gcagagccaa gcatcgtagg cgggcgcgag cggagcgata
      721 gaagaaagaa ggcgagtggg ctgggcagtc atggtgctgc cccattagcc cgtgctgtct
      781 gcgggatcaa gctgcgctct tggccggttg caacgcctcc gcccgaatcg cccgaggtac
      841 gccgaacagc gcgccctggc caagtgtcac acccgccgcc tggagctgcg tgagctgatc
      901 ctcgctcgcg aggccgactg cgacgacagt catcttcgcg tctctcaagc gcttgcaagc
      961 ctcagcaccc gagacgacgc cgtccagccc cgccaacccg tcaatcatcg actgagccgg
     1021 caagcgcacg aatatgaagc ctgaggactg aaggtcaggc aggtcgagat cgaggctcgt
     1081 cacctcgtcg accgcaaagc ggaacccatt ctccgaaagc tgccggatgg tcttccattg
     1141 cccctctgtg agctcgccca attcggactg ggtgatggcc atcaccagcc gctcgcgcag
     1201 gctgggcacc tgacggaatg cctggccgaa ctcttggagg aagcgctcat ccgccaacga
     1261 gtcggccgtt acgatcgcaa acagggaacc gttggtcttc cgctcgtcca tgtgccgcgc
     1321 caccatggcg gcgcgcgcga tgcacgttgc gtcgatgaca ggaagcagcc ctgcttttcg
     1381 tgccatcggg atatagtccc gaggacccag cccgtcggca gttccctgac gcaggcgcaa
     1441 ggaaacctcg aagtgccgcg ctttccggtc cgcgagactg acgatcggct ccaggaacac
     1501 gtcgagcttg tgcgcggcaa gagaaccggc cattgccgcc atcacggtat gttccggacg
     1561 aagcgccggc ggcagagggc ctttggcatt tgggttttcg cgataaggcg gcaactgccc
     1621 cttttcgatt gcagcgaggg ccgcttcgct gggcttccgc atctcgtcgg ctactgcccg
     1681 gagtgcgcca acagaagccg agatggcatc ctcctggctg agggccggga ccgtctcctt
     1741 ctcctccgat gcctcccccg tggttgcctc atcggacttg ctccgcggct gggagatctc
     1801 ctccgcgaac ttacggatca tcttgctgat ctgttcgacc tcagcctcag caggcgcatc
     1861 atccgtgtct gagggcgcgg gcgacgacga ggaggctgaa gtttcccccg aggtgagcgt
     1921 tgcagatggc tttgccgcct ccagcggtga ggctggcgcg gttgcggtcg attgctccgc
     1981 catcgtcggt gccgggcttg gaggggccgg cggcgcagaa ggcgcgccgc ggacgac
//
LOCUS       DASUMG010000443         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1101407, whole genome shotgun sequence.
ACCESSION   DASUMG010000443 DASUMG010000000
VERSION     DASUMG010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101407"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1585
                     /gene="nhaA"
                     /locus_tag="VFX46_07305"
     CDS             305..1585
                     /gene="nhaA"
                     /locus_tag="VFX46_07305"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5600425.1"
                     /translation="MAVRSATDRSSRLLEFLRTEEASGVFLLAAALVALVWANSGFAE
                     AYHQLWSTIATLGIGSVTISLDLQGWVNEGLMTLFFLVVGLEIKREVTTGELRDPKTL
                     ALPIMAAIGGMVVPAGIFLIVNVGNGTADGWGIPVATDIAFALGILTLAASRAPVSVR
                     SFLLTLAIVDDIGAILLIAVFYSGGLSAGWILTAAAIVALLFLMRIVRVTSTTAYVLL
                     GIALWVALFESGVHPTLTGVVLGLMAPAEPIARSTRAGTKVRQVLEEAAEQPDSEADA
                     YWIAVGEVSRTAVSPLDRAEASLHPCTSRFVVPTFALANSGVELSGGAVAEAVTSPLG
                     LGILLGLTLGKPIGIGGALWATTRMRLGRLPDEFTPRMVLGVALLGGVGFTIALFIAE
                     LALAGDGEIATATLAVLMASTIAGLMGGAVLRTR"
BASE COUNT          333 a          699 c          739 g          450 t
ORIGIN      
        1 ccatcgacgc cgacccggct cgaccctcgg gaggaggtga gggaggtttc agactcagcc
       61 gaccggctgc atgcctggct cgatcgagtg tcaatgcggc tccttcggga acacaaccgg
      121 actcgcgggg ccgcgtcttg actgcgtaac gctaagggcc tacggacagg ctcaccctag
      181 tggttcacgg cagcctgccc accacgtatt caggcgaagg gtgcgtccgt cgaccgcgct
      241 cggtatgatg agctgagaag tctattggtg cgccgggaag cctggtcggc agaagaggtc
      301 ggccatggct gtgcgctccg caacagatcg ctcctctcga ctcctggagt ttctgcgaac
      361 cgaggaggct tccggggtct tccttctcgc ggccgcgctt gtggcgcttg tctgggcaaa
      421 ttccgggttc gcagaggcgt accaccagtt gtggagcaca attgcgaccc tgggaatcgg
      481 cagtgtgacc atcagcctcg accttcaggg ctgggtcaac gaaggcctca tgacgctgtt
      541 cttccttgtc gtcggtctcg aaatcaagcg cgaggtcacg acgggcgaac tgcgcgaccc
      601 gaagacgctt gccctgccga tcatggccgc gatcggtggc atggtcgttc ccgcggggat
      661 cttcctcatc gtcaacgtgg ggaacggaac agcggacggg tggggcatcc ctgttgctac
      721 ggacatcgcc ttcgcgctcg gcatcctgac gcttgccgcg tcccgggctc cggtctccgt
      781 gcgatcgttc ctcctaacgc tcgccatcgt ggacgatatc ggggccatcc tcctgatcgc
      841 cgtcttctac tcgggcggtc tgtccgcggg atggatcctg acggcggcgg cgatcgtggc
      901 gctgctgttc ctgatgcgga tcgtccgcgt cacatcgacg accgcgtacg ttcttctggg
      961 gattgcgctc tgggtcgccc tctttgagtc cggtgtccac ccgaccttga ccggcgtggt
     1021 tctcggactc atggcaccgg ccgagccgat cgctcggagc acgagggctg gcacgaaggt
     1081 gcggcaagtc ctggaggaag ccgcggagca gccagacagc gaggccgatg cctactggat
     1141 cgccgtgggc gaagtgtcaa ggacggcggt gtcgcccctg gatcgcgccg aagcgtcgct
     1201 tcacccgtgc acgagtaggt tcgtggtgcc gacattcgcg ctcgccaact ccggcgtcga
     1261 gttgagcggt ggcgcggtag ccgaggccgt aacaagtccg cttggcctcg gcatccttct
     1321 tggactgacg ttgggaaagc cgatcggcat cggcggggct ctctgggcca cgacgcggat
     1381 gaggcttggc cgactgccag atgagttcac tccgcgcatg gtcctcggag tggccctgct
     1441 gggaggagtc ggcttcacga ttgcgctctt catcgccgag ctggctttgg ctggcgacgg
     1501 cgagatcgcc accgccaccc tcgctgtgct gatggcgtcg acgattgccg ggctgatggg
     1561 tggtgccgtc ctgcggacgc gttgactatg tcaccgcacg ctgcaagtca tggacgcgcc
     1621 gtcttggttc tggcgatcgc cgtcctcccg tggatcgccc tttcgcgagt cggcgaatgg
     1681 ggcacctcat ccggcgtttc tcttcggagg gaaggtggcg caatcgaggc gctcatgtcg
     1741 cgctcggtcg ttatgccgga ccgagtgctc ttctccgcca ccgggagacg cgccgacggc
     1801 acgcggcaca tctctccgac accaccccac tcgattgctg tgtccgccct tccgcctccc
     1861 ggttgggaac tctgtctgag gggttccgtc tcttggacgg agccgaccgt gcttcatcca
     1921 acgtgccgag gcccgcctgg gcctgcgtcc ggctcgctgt tcctgccttt ggaaccaagt
     1981 cattcgcagc gcggacttgc ctttgggtga gccgccattc gcttaggaag gaagacaaga
     2041 tgaccacggc tgagtcagtg cgagacctgg cgctttccct cgctcgctcg ggtctcgagc
     2101 gagaggacgc ggttcgcgag cttgaaggcg cgtgtgaggg gcgccgtgtt gccgtcgtaa
     2161 gagcccgtca aatgattgaa acgtctcttg acaacggctc tgaagggtct gatgcagccg
     2221 g
//
LOCUS       DASUMG010000444         2353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_379528, whole genome shotgun sequence.
ACCESSION   DASUMG010000444 DASUMG010000000
VERSION     DASUMG010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379528"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFX46_07310"
     CDS             complement(<1..581)
                     /locus_tag="VFX46_07310"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600426.1"
                     /translation="MESHTSLRTIGQGPLKAMLMGRRRFLLTSLAGALATPLGGGTHG
                     AERVWRVGILWGIRPGPEWTAGGPFFGPMRELGWVEGQNIVFEQRHANEQPERLPMLA
                     RELIASKVDVIVTAPSSATRAAMEATATIPIVFAEVGKPVRRGFVPSLARPGGNVTGL
                     ADISLELIPKRLELLKELVPGATRAALLVEPGF"
     gene            complement(1258..1905)
                     /locus_tag="VFX46_07315"
     CDS             complement(1258..1905)
                     /locus_tag="VFX46_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600427.1"
                     /translation="MDVSSGLRADGASSASIARSLLCFRPAEYPSPMVSKAVAGLGAL
                     VLWASTSLAQAPESQRAETYEAVTVDADGRLVIRTADRRTIVLRREGEQTAFANPIIS
                     AGGAAVGAQAEFKNCCTSYDIPLELVIYANGTVHRFTGIGLPIFRWHFVDGGSRVAFA
                     QEPVHFGCSVHYELRDVQSERLIDSTDVPEPCGQMPHPPPVTIPSWVAELNAETR"
     gene            1904..>2353
                     /locus_tag="VFX46_07320"
     CDS             1904..>2353
                     /locus_tag="VFX46_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600428.1"
                     /translation="MRILATGPSGPPEELEALRAAGHELVIGRPLDMPGRRAYTAAEL
                     IEACRDVEVVLASHLETISAAVLGAAPRLRLVIVPFIGTDKIDIGAASRLGILVANSP
                     TPENFVAVAEATIGVTLMLLKRIKHNEAKLRRGEWAKREDRGDFLFGK"
BASE COUNT          420 a          821 c          735 g          377 t
ORIGIN      
        1 tagaatccag gttccacaag aagcgcagcc cgcgtggcgc ctggcaccag ctccttcaat
       61 agctccagcc gcttcggtat gagctccaac gagatatccg ctagccctgt gacgttcccc
      121 cctggtcggg ccaggctcgg aacaaaaccc cgtctcactg gtttaccgac ctcggcaaac
      181 acgatcggga tcgtcgccgt cgcctccatc gccgcgcgag tggcggaact gggagcggtc
      241 acgatcacgt cgaccttgga cgcgatgagc tctcgcgcaa gcatcggcaa ccgctctggc
      301 tgttcgttcg catggcgctg ctcgaaaaca atgttttgcc cctcgaccca gcccaactca
      361 cgcatgggtc cgaagaacgg gccgccagcg gtccactcgg gacctggtcg aatgccccac
      421 agaataccga cacgccacac gcgctcagcc ccgtgcgtgc caccgccgag tggcgtcgcg
      481 agggcaccag ctagcgaggt cagcaggaag cggcgacggc ccatgagcat cgccttcaaa
      541 gggccctgcc cgatcgtgcg aagactcgtg tggctttcca tcacggctta tcccccagct
      601 tctccaagcc gccccgagca gcgcccggat ctccgcgtcg cgcgcctgtc gtgcggcgtt
      661 cagtcggtac cgactctcac gcgagcccgg caccgggccg aacagcccgt gttctggcgt
      721 ccgcgcaacg gccctccgca ccctccacac cgggcacgtg cggattctac gggcattggg
      781 gaccttgagg tagaggggta ttggctagag ggtcggcgcc tcgaagcact tgccaaccac
      841 gcggctgagg tagatggacg ttaattcgga cggggaccga gatcggggaa tgacacgtta
      901 ggcactgcgc cggacgagtg taccagtcct gtaggtcgca gccctcacgc cgcgccccca
      961 accagcgcgc atgcatcggc gagccgttcg tgcttgtcga tgtgctcgcg cgggcgccag
     1021 ccctcgcgct cggcgtacgc gacgagctag cgctcaccgc gcgcggttaa ggcatgggcg
     1081 cgcgcgtgta atctcggccc cgtgccacat gtgaccaagt tcggatattc ttggcgcgcc
     1141 agccccgaac ggggacaaaa cgggggcaat tcatcgcatc ggtccggcgg aaagagcgta
     1201 acgtccgcga gctcgaagct gcatagttga cagtctgcaa gaagccgtag cctccgctta
     1261 gcgcgtctcg gcattgagct cggccaccca cgaggggatg gtcaccggtg gcgggtgagg
     1321 catctgcccg cagggctccg gcacgtccgt cgagtcgatg aggcgctccg actgaacgtc
     1381 tctgagctcg taatggaccg agcagccgaa gtgaacgggc tcctgtgcga atgccacgcg
     1441 cgacccaccg tcgacgaagt gccacctgaa gatcgggagc ccgatgccgg tgaaccgatg
     1501 cactgtgccg ttggcgtaga tcacgagctc gagcgggatg tcgtacgacg tgcagcagtt
     1561 cttgaattcg gcctgcgccc ccactgccgc accgcccgct gagatgatcg ggttcgcaaa
     1621 cgccgtctgc tcgccttctc ttcgcaagac tatcgtccgg cgatcggccg tgcggatgac
     1681 caggcgaccg tccgcatcaa cggtcacggc ttcgtaggtc tctgcccgct ggctctccgg
     1741 ggcttgagcc aagctcgtcg acgcccagag caccaacgcc cccagccccg ccaccgcctt
     1801 cgacaccatc ggcgaaggat actctgccgg ccggaaacag agtagacttc gcgcgatcga
     1861 ggcagaggag gcaccatctg cacgcagccc tgaggagaca tccatgagga tcctcgccac
     1921 cggaccaagc ggtccccccg aggagctgga ggcgctgcgc gccgccggcc acgagctcgt
     1981 gatcggccga cccctcgaca tgccggggcg tcgcgcctac accgcggccg agctgatcga
     2041 ggcctgccgg gacgtcgagg tcgtcctcgc ttcgcacttg gagaccatct ccgccgccgt
     2101 gctgggcgcg gctccgcgcc tccgcctggt catcgtgccc ttcatcggaa cggacaagat
     2161 cgacatcggg gccgcctcgc gcctgggcat tctcgtggcc aacagcccga caccggagaa
     2221 cttcgtcgcg gtcgccgagg ccacgatcgg cgtcaccctg atgctgctca agcgcatcaa
     2281 gcacaacgaa gcgaagctcc gccgcggcga gtgggccaag cgtgaagacc gcggcgattt
     2341 cctcttcggc aag
//
LOCUS       DASUMG010000445         2555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1182592, whole genome shotgun sequence.
ACCESSION   DASUMG010000445 DASUMG010000000
VERSION     DASUMG010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182592"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="VFX46_07325"
     CDS             complement(<1..2379)
                     /locus_tag="VFX46_07325"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HEX5600429.1"
                     /translation="MSSRLPRIFVLVLTCVGCLMIALGLPRVAYANEPAGSTASPGTT
                     KAQQPESPTPSPFFPAELAQRIVRLASDVENAAKGIQRVKDRESGLAGQRLELERIEV
                     EAQELIAALKPRLSAVTAQLQKLGPPPEKDAAPEAPNVASERERLTSIQSNVEGAIKS
                     AELALVRSRQLMRLVQHHRQALFARELMQRTGSPLWLSTWQDLAAELPRTSRQISNLA
                     KAWWQQARDKPLSLVLVVAMAAGVYGVLRLALSVIGSRMRVRGEGWAAYFPNTGDAIW
                     RAATGALPPLSAAITLYGGLAGSGLLTATVGELALAGLKAFAVFIGVRAVAEVALAPT
                     PSGFRLLDLAGAPKLLRLARVLAALIAIETLLRDVVRILYLPLEIGVVQTTLTNLAFA
                     AVLAAFVRIPLGSREAAVLQPPSRSRPLWIKVPVAAAALMILGTTLAGYVALGQFIST
                     QAMLAGLTLFLLLLAHAIIRRAVGALADGERPMGRILETRLGLDSERTSYVTRLIIGV
                     VDTVLLLVAVPLILLTWGYTRDDFIDWLKVGIVGFEIGRFQISLVRILLALGLFLILL
                     FLTRAFQRWLDRSVLMPARIDRSIAHSVLMGVGYTGVGLAIIFALSYAGLDFTQLAIV
                     AGALSLGIGFGLQAIFNNFVSGIILLIERPVKVGDWIVVNGQEGFVRRINVRATEIET
                     FDRSSLIIPNSELITGTVMNWTHRNAIGRLVVYVRVSYKADPEQVIAILRRVAEDSPS
                     ILREPEPTVVFEDFGESAMIFSLRAFVPDVMRRLAVQTELRLAIAKSFREAGI"
BASE COUNT          528 a          802 c          793 g          432 t
ORIGIN      
        1 gatccctgcc tcgcggaacg acttggcaat tgccagccgc aactccgtct gcaccgcaag
       61 ccgccgcatc acatcgggca caaaggcgcg gagggagaag atcatcgcgc tctctccgaa
      121 atcctcgaaa acgactgtcg gctcaggctc tctcaggatg gatggggagt cctccgcgac
      181 gcggcggaga atagcgataa cctgctccgg atccgctttg taagaaacgc ggacgtacac
      241 cacgaggcgg ccaatcgcgt tgcggtgggt ccagttcatc acggttccgg taatcagctc
      301 ggagttgggg atgatcaggc tcgatcgatc gaacgtctcg atctcggtcg cgcgcacatt
      361 gatgcggcgc acaaagcctt cctggccgtt aacgacgatc cagtcgccga ccttcaccgg
      421 tctttcgatg agcagaatga ttcccgagac gaagttgttg aagatggcct gcagtccgaa
      481 gccgatcccg agggagagcg cgccggccac gatggcgagc tgcgtaaagt cgagccccgc
      541 atatgacagc gcgaaaatga tcgccagacc gacaccggtg taaccgacac ccatcagcac
      601 cgaatgtgcg atggacctgt cgatccgcgc aggcatcaga acggaccggt caagccacct
      661 ctggaatgcg cgcgtaagga acagcaggat gaggaaaagg ccgagcgcga gcaggatgcg
      721 cacaagcgag atctggaatc ggccaatctc gaatccgacg attccaactt tcagccagtc
      781 gataaaatcg tctcgtgtgt agccccaagt gagcaggatc agcggcaccg cgaccagcag
      841 gagaacggtg tccacgaccc caatgatcag gcgagtgaca tagctcgtcc gctcgctgtc
      901 gaggccaagt ctggtttcca ggatgcgtcc catcgggcgc tcgccatcgg caagcgcgcc
      961 taccgcgcga cggattattg cgtgagctaa cagcagtaga aatagcgtca gccccgccag
     1021 catagcttgt gtggaaatga actgtcccag ggccacgtaa ccggccagcg tggtccccag
     1081 gatcatcagc gccgcagccg caaccggcac ctttatccac agcggtcgag aacgagacgg
     1141 cggctggagg accgcggctt cgcggctgcc gagagggatg cgtacgaacg cagccagaac
     1201 ggcggcgaag gcaaggttgg tcagtgtcgt ttgcacgacg ccgatctcga gaggcaggta
     1261 aagaatccgc accacatcgc ggagcagcgt ttcgatggcg atgagggcgg caagcaccct
     1321 cgccagacgt aacagcttcg gcgcgccggc taggtccagg agccgaaaac ctgacggagt
     1381 cggggccaag gctacttcgg caactgccct caccccgatg aacaccgcga atgctttcag
     1441 ccctgcgagc gcaagctcac cgaccgttgc cgtcagcagt cccgagccgg cgagcccccc
     1501 gtaaagcgtg atcgccgcgg acagaggcgg caacgcgcct gtggcggctc tccatattgc
     1561 atcgcccgta ttcgggaagt aggcggccca gccctcacca cggacccgca tccggcttcc
     1621 gattaccgaa agggcgaggc gtagcactcc ataaacgcca gctgccatcg ccacgaccag
     1681 cacgagcgac aacggcttgt cgcgcgcctg ctgccaccat gctttcgcga gattgctgat
     1741 ttgtcggctc gtgcgcggca gctcggctgc gagatcctgc caagttgaaa gccacagcgg
     1801 gcttccggtt cgctgcatca gctcgcgggc gaagagggcc tgccgatgat gttgcaccag
     1861 cctcatgagc tgccgcgacc ggaccagggc cagctccgca ctcttgatgg cgccctctac
     1921 attcgactgg atcgaagtca gccgctcccg ttcgctggcg acgttcggcg cctcgggggc
     1981 cgcatccttt tcgggagggg gaccgagctt ttgaagctgt gccgtgacag ctgaaaggcg
     2041 aggtttgagc gcagcaatga gctcctgggc ctcgacctcg atgcgttcca gctcgagtcg
     2101 ttgtcctgca agcccgctct cccgatcctt gacgcgctga atgcctttgg cggcgttctc
     2161 gacatcggag gccaggcgca cgatgcgttg ggccagctca gcggggaaaa aaggcgatgg
     2221 tgtcggggac tcaggctgct gcgctttagt cgtgccggga gatgcggtcg accccgcggg
     2281 ctcattggca tatgcaaccc tcggcaggcc aagagcaatc atgaggcagc cgacgcaggt
     2341 cagcacaagg acgaaaatcc tcggaagtcg tgatgacatg ccccggctcc agtgttggcg
     2401 gctcacgcct cgaccacggt gctcgaccgt gtgcgccgct ggaaaatcga ctccgcaggt
     2461 tcatgcgtgg tctcgggcat ctcgggtggt tgaatgccgg tctcgatcac cacgtcgaca
     2521 ggtcccccgg tcatgattag atgctccaca ccgtc
//
LOCUS       DASUMG010000446         3537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_219371, whole genome shotgun sequence.
ACCESSION   DASUMG010000446 DASUMG010000000
VERSION     DASUMG010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219371"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFX46_07330"
     CDS             complement(<1..446)
                     /locus_tag="VFX46_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600430.1"
                     /translation="MAKVFRESWQLAYRGIIPHLHLECMIRRRGPEWWASAVRSGDNI
                     LVLEVNGEVAGYATWGSARGRSSYQGEIYELYLAPVYQGLGFGELLFEACRSAMDERR
                     IKGLICWALAENTPAIDFYWRRGGRPIARAYDRIGGVKLEKIAFAW"
     gene            complement(648..1103)
                     /locus_tag="VFX46_07335"
     CDS             complement(648..1103)
                     /locus_tag="VFX46_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEX5600431.1"
                     /translation="MNEQFTQQAQEAFNAAKEARIPENVQAMAAESVAKGRETYQKLN
                     AAAKDSLKAFEEVITTAQAGARAISEKVLNNTAANAEAAFDAAQAIARARTLPEAIRL
                     QTDFLQKQFTVASAQTKELFELSTKVAQQTLESISSAANRTSASFKFNS"
     gene            complement(1278..2603)
                     /locus_tag="VFX46_07340"
     CDS             complement(1278..2603)
                     /locus_tag="VFX46_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5600432.1"
                     /translation="MTGLCPASRISWPEGFQVTKEHELAAAFPETGEGHLFITGRAGT
                     GKSTLLRCLREIIADQGIVLAPTGLAAINVGGQTIHSFFGFPPRLIGPDDIRRSRNAR
                     IMRQLKFLIIDEVSMVRSDLMWAIDQALRVNRGRPREPFGGVRLLLFGDLHQLPPVVQ
                     EAAEAEHLEARFGGPFFFDVPTLQDGAGTAYLELKHVFRQDDADLVSVLDRIREGEAG
                     PDDLELLNSRVRPIRNLAEGDAFVILTPTNAAANRINAAYLEALPGQAYAYEASLTGD
                     FSDAAQPTDSILLLKPGAKVILLRNDPDRRWVNGTIARISRLSESQVWVEIDGKEHEV
                     EPVAWENRRYAYDQAAEKIVETVAGTFKQFPLRLAWALTIHKSQGLTLDRVYIDLGRG
                     TFAHGQAYVAISRCRSLDGLAFARPLRPQDVLFDPATLRYRELFPALAA"
     gene            complement(2620..>3537)
                     /locus_tag="VFX46_07345"
     CDS             complement(2620..>3537)
                     /locus_tag="VFX46_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX5600433.1"
                     /translation="LSMTFRINDGPLAGQEGDKVQSRVIRERLFREAERNIAIRVIES
                     EEKDAFEVAGRGELQLAILIETMRREGFELTVSRPRVVFKTDPETGQRLEPIEEVIID
                     VDEQYSGVVVQKISERRGELIEMRPSGGGRLRMVFHVPTRGLLGYHSELLSDTRGTAV
                     MNRLFHGYAPYRGEIQGRRTGVLVSNGDGEAVAYALYNLEDRGALIIGPQTRVYQGMI
                     VGEHSRENDLDVNVLKGKKLTNIRAAGKDDNILLTPPMKMTLERALSYIADDELVEVT
                     PKSIRLRKRWLDPNMRKKMERMKDAKAVA"
BASE COUNT          658 a         1102 c         1070 g          707 t
ORIGIN      
        1 ttccatgcga acgcgatttt ttcgagcttg acgcccccga tccgatcgta ggctctggcg
       61 atcggccggc cgccacgccg ccaatagaag tcgatggccg gggtgttttc ggctaaagcc
      121 cagcagatca ggcctttgat cctgcgctca tccatggcgc tgcgacaggc ttcgaagaga
      181 agctcgccaa acccgaggcc ctgataaacg ggggcgaggt agagctcgta aatttcgccc
      241 tgataagagc tgcggccgcg cgccgaaccc cacgtggcgt aaccagcaac ttcgccattg
      301 acttcgagca cgaggatatt gtcacccgag cgcacggcac tggcccacca ttccggaccg
      361 cggcggcgga tcatgcattc gagatgaaga tgcgggatga tgccccggta tgcgagttgc
      421 caggactcgc gaaagacctt tgccaatgct tccgcatcgg atggcctgcc gcgccttact
      481 cgaacttttg agctggaccc caacatgagt ccactataaa gcgcggcaga aaatgtattt
      541 caacgttgtt gtcgcggctt cgttccgccc gcgttcacaa gtcacaggtg cgagggtccg
      601 aagacagcct ttcggaccct cgcaggattg tcgtcaccaa gaagagctca cgaattgaac
      661 ttgaagctcg cgctcgtacg atttgccgcc gagctgatgc tctccagcgt ttgctgagcg
      721 actttcgtcg aaagctcgaa aagctccttg gtctgggcgc tcgcgacggt gaactgcttc
      781 tgcaggaaat ccgtctgcag gcggatggcc tccggcagcg tgcgagcgcg ggcaatggcc
      841 tgagctgcgt cgaacgcggc ctcggcattt gccgcggtgt tgttcaagac cttctcgctg
      901 atggcacgcg cgccggcctg agcggtcgtg atcacttcct cgaacgcctt gaggctgtcc
      961 tttgccgcgg cattgagctt ctggtaggtc tcgcggccct tggcgacgct ctccgcggcc
     1021 atggcctgta cgttttccgg gatgcgagcc tctttggccg cgttgaatgc ttcttgtgcc
     1081 tgctgcgtga actgctcgtt catgaccgtc tcctaaggag cctcgagggg ttgtgaccaa
     1141 tgtcagcgga ggtggggaat cgctccgcca aatccgactg ctccctacct aatacgttct
     1201 attgtgcgat gcaacattca ttgtgcatcg cgaaaatgaa caaactgtcg cacctcgaac
     1261 tgtaggacgt cagaaaatca agcggcgagt gccggaaaaa gctcgcgata gcgcagggtt
     1321 gccgggtcga agagcacgtc ttgcggcctt agcggccggg caaacgcaag gccgtccaga
     1381 gatctgcacc gcgagatggc cacgtaggcc tgcccgtggg cgaacgtacc tcgccccagg
     1441 tctatgtaga cccgatccaa agtcagccct tggctcttgt ggatggtgag ggcccaagcc
     1501 agccgcaacg gaaactgctt gaaggtgcca gcaaccgttt cgacgatctt ctctgccgcc
     1561 tggtcgtagg cataacgccg gttctcccaa gcgacaggct cgacctcgtg ctccttgccg
     1621 tcgatctcca cccacacctg gctctcgctg agccggctga tgcgggcaat cgtgccgttg
     1681 acccagcgcc ggtccggatc attccgcagc agaatgacct ttgccccagg cttgagcaga
     1741 agaatgctat ccgtcggttg ggcagcgtcg ctgaagtcac cggtcaggct ggcttcgtag
     1801 gcataagcct gacccggaag agcttccaaa taagccgcgt tgatccgatt ggccgcggca
     1861 ttcgtgggcg tcaggatgac gaaggcatcg ccctcagcaa gattgcggat aggacgtaca
     1921 cggctgttaa gaagttccag atcatccggg ccggcctcgc cctcccgtat ccggtctagc
     1981 acgctcacga gatcagcgtc atcctggcgg aacacgtgct tgagctcgag gtacgctgtg
     2041 ccggcgccgt cctgcagcgt gggcacgtca aagaagaacg gtccgccgaa acgagcttcg
     2101 aggtgttccg cctcagccgc ttcctgaacg acgggcggga gctggtgaag gtcgccgaac
     2161 agcaagaggc gcacaccgcc gaaaggctcg cggggccgtc ctcgattgac gcgcagcgcc
     2221 tggtcgatcg cccacatcag atcggagcga accatcgaca cttcgtcgat gatgaggaac
     2281 ttgagctgcc gcatgatgcg ggcgttgcgg ctgcggcgga tgtcgtctgg cccgatcaga
     2341 cggggaggaa agccgaaaaa ggaatggatg gtctggccac cgacattgat ggcggcaagc
     2401 ccggtgggcg cgagcacgat gccctgatct gcgatgattt cccgcaggca gcgcagcagc
     2461 gtggatttgc cagtccctgc acggccggtg atgaaaagat gaccttcacc ggtctcggga
     2521 aaggctgccg ctagctcgtg ctctttggtg acctgaaacc cctctggcca tgaaatcctg
     2581 ctggctgggc aaagccccgt caccgcatct gtctcccact caggcgacgg ccttcgcgtc
     2641 tttcatgcgc tccatcttct tccgcatgtt ggggtcgagc cagcgcttgc gcaagcggat
     2701 ggacttgggc gtgacctcca ccagctcgtc atccgcgatg taggagagag cgcgttccag
     2761 cgtcatcttc atcggcgggg tgagcagtat gttgtcatcc ttgcctgccg cgcggatgtt
     2821 ggtcagcttc ttgcccttga gcacgttcac gtcgaggtcg ttctcgcgtg agtgctcgcc
     2881 tacgatcatg ccctggtaga cgcgcgtttg cggcccgatg atcagcgccc cgcggtcctc
     2941 caggttgtag agcgcatagg ccacagcctc accgtcgcca ttggagacca gcacgcccgt
     3001 gcgccggccc tggatttcgc ctctatacgg agcgtagccg tggaacaggc ggttcatgac
     3061 ggccgtgccg cgcgtgtcgg aaagcagctc actgtgatag ccaagcaagc cacgcgttgg
     3121 cacgtggaac accatcctca ggcgcccgcc acctgaaggg cgcatctcga tcaactcgcc
     3181 gcgacgctcg gagattttct ggacgacgac gccactgtac tgctcgtcga cgtcgatgat
     3241 cacctcctcg atgggttcaa gtcgctggcc cgtttccgga tcagtcttga acactacccg
     3301 cggccgggac accgtcagct cgaagccctc ccgccgcatg gtctcgatca gaatggcaag
     3361 ctgcagctca ccgcggcctg ccacctcgaa ggcgtccttc tcctcggact ctattacgcg
     3421 aatggcgatg ttccgctcgg cctcgcggaa aagccgctct cggatgacgc ggctctggac
     3481 cttgtcgcct tcctgcccgg caagcgggcc gtcgttgatg cggaaggtca tcgacag
//
LOCUS       DASUMG010000447         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1102055, whole genome shotgun sequence.
ACCESSION   DASUMG010000447 DASUMG010000000
VERSION     DASUMG010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102055"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1134)
                     /locus_tag="VFX46_07350"
     CDS             complement(139..1134)
                     /locus_tag="VFX46_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600434.1"
                     /translation="MGSVIRAFDLESGYQLCQGGSFNLARGLMEAFIAAGGTFEPQVN
                     IDRILIENGRATGIELLGGQTVRASQFVASTLDVHQTFESLIGREQLPGTFLKKLDGF
                     QYTAWSLFGLHLALNESPRFAAEKSDPNINRSLKWSIGAETMEDLFSAHQDVQAGRVP
                     KLVQFGSGPLSLLDPTQAPPGKHTTYCWHVMPLNPDLGGQSYEAFKEEFSDRIVECFA
                     RYCPNMTSKNILGRYVYTGREYVQEMINMRGGDIFMGAFNAEQVMYNHFGYRSPIPNL
                     YSAGSAGHPGGAISGGAGYITAGIIANDLGLKPWWTPWDARAALEQLPQLERGAA"
     gene            complement(1122..1775)
                     /locus_tag="VFX46_07355"
     CDS             complement(1122..1775)
                     /locus_tag="VFX46_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX5600435.1"
                     /translation="MSKALHKTYDGIIIGAGHHGLILGTYLARAGLDILLVDRRLTYG
                     GGLCTREVTLPGFYHNLHSINHFHISEAPWFKDLNLDERVTYITPRYEFGQAHQDGSA
                     LVLGRDLEESVANIARFSKKDAQTFRDWNRKAEKITAEIFLPERYAEPLPKQEREALL
                     SRTALGREFLEVSNRQPLDVVRELFENEHVRLLFLFKVSLLAPGSWIPSRRPHRWAR"
     gene            1994..>2450
                     /locus_tag="VFX46_07360"
     CDS             1994..>2450
                     /locus_tag="VFX46_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600436.1"
                     /translation="MRNTLLAAGAALLLAASPAAAQQKTIKIGFISTFSGPVAVIGND
                     MRNSFELALDHLGRKMGGIPVEVIYEDDGFKPEVGKQKTDKLIESDKVDFITGYIWSN
                     VLLASLKSAVDSKTFLITANAGPSQLAGELCSPYVFSSSWNNDQTPQSVG"
BASE COUNT          480 a          730 c          759 g          481 t
ORIGIN      
        1 tgagttctgc gatgacggcc cgtgcaccgg ccgttgcgaa cgattttgcg aacacgcgcc
       61 caatgccctg gcccgcgccg gttacaataa cgacgcggtc cttcaggccc ggaaacacgg
      121 cattctgcaa ttcggcgctc atgccgcccc ccgctccagc tgcggcaatt gttccagcgc
      181 ggcgcgcgca tcccacggcg tccaccaggg cttgagtccg agatcgtttg cgatgatgcc
      241 cgccgtaatg tagccggcgc cgccggaaat cgcgccaccc gggtgaccgg ccgagcccgc
      301 actgtacaga ttcgggatcg gcgaacggta gccgaagtgg ttgtacatga cctgctcggc
      361 gttgaatgcg cccatgaaga tgtcgccgcc gcgcatgttg atcatctcct gcacatattc
      421 gcggccggtg tagacataac gtccaagaat gttcttgctt gtcatgttcg ggcagtaccg
      481 cgcaaagcat tcgacaattc tgtcggagaa ttcctccttg aaggcctcgt agctttgtcc
      541 gccgagatcg ggattgagcg gcatgacgtg ccagcaatag gtcgtgtgct tgcccggcgg
      601 ggcctgtgtc gggtcgagca ggctgagagg ccccgatccg aactgcacca gcttcggcac
      661 gcggccggct tgcacgtcct gatgagccga gaacaggtcc tccatggtct cggcgccgat
      721 gctccacttc agcgagcggt tgatgttcgg atcggacttc tcggcggcga aacgcgggct
      781 ttcgttgagc gcgagatgca gcccgaacag gctccaagcg gtatattgga aaccgtcgag
      841 tttcttgagg aaggtgccag gcagctgttc gcgcccgatc aggctctcga aagtctggtg
      901 cacgtcgagc gtcgaagcca caaattggct ggcacggacc gtctggccgc cgagcagttc
      961 gataccggtt gcgcgtccgt tctcgatgag gatgcgatcg atattcacct gcggctcgaa
     1021 agtgccgccc gcggcgatga aggcctccat taggccgcgc gccagattga acgagccgcc
     1081 ttggcagagc tggtagccgc tttcgaggtc gaaggcgcgg atcaccgagc ccatcggtga
     1141 ggtcttcgag agggtatcca cgagccaggt gccaacaaag acaccttgaa cagaaacagc
     1201 aaccgcacgt gttcgttctc gaacagctcg cggacgacgt cgagcggctg ccgattggac
     1261 acctccagaa attcacgtcc taaggccgtg cgcgacagca gcgcttcgcg ctcctgtttc
     1321 ggcaaaggtt cggcatagcg ctccggaagg aagatctccg ccgtgatttt ttctgccttg
     1381 cgattccagt cgcggaaagt ttgcgcgtcc tttttcgaga agcgcgcgat gttggcaacc
     1441 gattcttcaa gatcgcgccc gagcacaagc gcgcttccat cctggtgtgc ctggccgaat
     1501 tcataacgtg gcgtaatgta ggtgacgcgc tcgtccaggt tcaaatcttt gaaccaaggc
     1561 gcctcgctga tgtggaaatg attgatggag tgcaggttgt gatagaagcc gggcagcgtt
     1621 acttcgcgtg tgcacaaacc gcccccgtaa gtcaggcggc gatccaccag tagaatgtcc
     1681 aggccggcgc gggcgagata ggtgcccagg atcaggccat ggtgtccggc accgataatg
     1741 atgccgtcat aggtcttgtg gagagccttg gacaaaggtc gaattcctcg aactatattt
     1801 cctgcggtct gaagcagggg ccgccgggct agactgcaag gcgtccggct gccgggtcaa
     1861 cgggcacaag tcgcacggcg tgggacgcca gcgggacgtc gggccgcatt ttcttcgggt
     1921 ggaccaatac ccgccgactt cggtacatta ggccatccaa aacacgggcc gaaatggcct
     1981 cggggaggaa cacatgcgta acacgctttt ggccgcgggc gccgctctgc tgctcgctgc
     2041 gagcccggct gcggcccaac agaaaacgat caagatcggt ttcatcagca cgttcagcgg
     2101 tccggtcgcg gtgatcggca acgacatgcg caattcgttc gagctggcgc tcgatcacct
     2161 cggtcgcaag atgggcggca ttccggtcga ggtcatctat gaagatgacg ggttcaagcc
     2221 ggaagtcggc aagcagaaga cggacaagct gattgagtcc gacaaggtcg atttcatcac
     2281 cggctatatc tggtcgaacg ttctgctcgc ctcgctgaag tcggcggtcg actccaagac
     2341 gttcctgatt acggccaacg ccggtccctc ccagctcgca ggcgagctgt gctcgccgta
     2401 cgtgttctcg agctcctgga acaacgacca gacgccacag tcggtcggca
//
LOCUS       DASUMG010000448         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_219905, whole genome shotgun sequence.
ACCESSION   DASUMG010000448 DASUMG010000000
VERSION     DASUMG010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219905"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1328
                     /gene="fabF"
                     /locus_tag="VFX46_07365"
     CDS             63..1328
                     /gene="fabF"
                     /locus_tag="VFX46_07365"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX5600437.1"
                     /translation="MRRVVITGLGLVTPLGCGVEETWSRLLAGQSGARRVDTFDVSDL
                     SCQIACMVPRGDGTNGTFNPNDWMEPKEQRKVDDFIIYAMAAAEQALNDAGYRPTTTE
                     EQERAGVLIGSGIGGLQGIADTAILLQEKGPRRVSPFFIPGRLINLASGQISIRYQLK
                     GPNHAVVTACSTGAHAIGDAARLIALGDADVMVAGGTESPICRLAIAGFAACRALSTN
                     FNDQPERASRPYDRDRDGFVMGEGAGVVVLEELEHARARGARIYAEVIGYGLSGDAYH
                     ITAPAEDGDGAYRCMKAAIKRAGIQPSDIDYINAHGTSTPMGDEIELAAVERLFGSAA
                     EKLSMSSTKSAIGHLLGAAGAVEAIFSVLAIRDNVAPPTLNLDNPSVDSVIDLVPHKP
                     RKRRIDTVLSNSFGFGGTNASLILRRFDA"
     gene            1664..>2016
                     /locus_tag="VFX46_07370"
     CDS             1664..>2016
                     /locus_tag="VFX46_07370"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX5600438.1"
                     /translation="MGPTLRFVNGFLTFSFLALAVAAGGVFWVSSELDKAGPLEEPRT
                     VVIRRGEGARDIARRLEREGFISSEHIFVAYYLARQVSGWFGGEPLNLKAGEYQLKPA
                     ISMREVANLLSEGKVA"
BASE COUNT          370 a          601 c          669 g          376 t
ORIGIN      
        1 ctctgcaaga cagtcggtgg cgttacgggg gctaccgggg cgccgccggt gaaggattgc
       61 gcatgcgacg agttgtcatc acgggattgg gcctcgtcac cccgctaggt tgcggcgtcg
      121 aggagacatg gtcacggctg ctggccggcc aaagcggtgc gcgtcgggtt gatacgttcg
      181 acgtttccga cctgtcatgc cagatcgcct gcatggtgcc gcgaggcgac gggacaaacg
      241 gcacgttcaa ccccaacgac tggatggagc ctaaggagca gcgcaaggtc gacgacttca
      301 tcatctacgc catggcagcg gcggagcagg cgctgaatga cgcgggctat aggccgacca
      361 cgaccgagga gcaggagcgc gccggcgttc taatcggctc cggtatcggt gggctgcagg
      421 gcattgccga cacggccatc ctgcttcagg agaaggggcc gcgcagggtt agcccgtttt
      481 tcattccggg ccggctgatc aatctcgcct ccgggcagat atcgatcaga tatcagctca
      541 agggccccaa ccacgcggtc gtcaccgctt gctcgaccgg agcgcacgcc atcggcgatg
      601 ctgcgcggct gatcgccttg ggtgatgccg atgtgatggt ggccggaggt acggaaagcc
      661 cgatctgtcg gcttgccatc gccggctttg ctgcctgccg tgcgctttcg accaacttca
      721 atgatcagcc ggagcgggcc tctcgcccat acgacagaga ccgcgatgga tttgtcatgg
      781 gcgaaggcgc cggcgtcgtc gtgctcgagg agttagagca cgcccgcgct cgtggcgcgc
      841 gaatctatgc cgaggtgatc gggtacggcc tgtcgggtga cgcctatcac atcacggcgc
      901 cggccgagga tggcgatggg gcctaccggt gcatgaaggc cgcgataaag cgcgctggca
      961 tccagccaag cgacattgac tacatcaacg cgcacgggac ctcgacgccg atgggcgacg
     1021 agatcgagct ggccgccgtc gaacgtctgt ttggcagcgc ggcggagaag ctctcgatgt
     1081 cgtcgacgaa atccgccatc ggtcaccttc ttggcgcggc cggtgcggtg gaagccatct
     1141 tcagtgttct cgcaattcgc gacaatgtgg ctccgccgac gttgaacctg gacaatcctt
     1201 ccgtcgacag cgtaatcgac ctcgttccgc acaagcctcg gaaacggcgg atcgacacag
     1261 ttctttcgaa ctcgttcggt ttcggaggaa ccaacgcttc tctcatcctc cggcgcttcg
     1321 atgcctgagg cgttcatcag taagcggcag agtaactgcg aagccacacg aggcttgccg
     1381 ttagctgccc gctgttgcca cattcctcaa tcatcttgcc aaccctgaat atagccgtcc
     1441 attcggtaga ctgatctgtg attcgggaga aagcctttgg tcgcgctagg gttggtgggg
     1501 gaactgcgag cgaatgctga aggctcggcc gccaagggaa cgacgacgga cgcaccggac
     1561 gcaccccccg ggcgtcatgc cgcgcagtcc ggctgaggcc cttgagccga cgcgcgcgcc
     1621 taccaggccg aaaggtccga gaacacgccg cgaggtgagt agattgggtc caacgctgcg
     1681 gttcgtgaac ggatttttga cgttcagctt ccttgcgctc gcagtggccg cgggtggcgt
     1741 gttctgggtt tcgtcggaat tggacaaggc aggcccgctg gaggagcctc gaacggtggt
     1801 catccgccgg ggcgaaggcg cgcgcgacat tgctcggcgg ctcgagcgag aagggttcat
     1861 ttcgagcgag cacatcttcg ttgcgtacta cttggcgcgc caggtatccg gctggttcgg
     1921 tggagaaccg ctcaatctga aggccggtga gtaccagttg aagcctgcca tcagcatgcg
     1981 cgaggtggcg aacctcctca gtgaaggcaa agtcgc
//
LOCUS       DASUMG010000449         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_781297, whole genome shotgun sequence.
ACCESSION   DASUMG010000449 DASUMG010000000
VERSION     DASUMG010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781297"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VFX46_07375"
     CDS             complement(<1..299)
                     /locus_tag="VFX46_07375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmO family methyltransferase"
                     /protein_id="HEX5600439.1"
                     /translation="MDQDATRAGELEVPLPERTDARLVFIGRIRTPFASRDDCPRQGS
                     PEGPVCRIEIDEPWRPGLVGLERYERAEVLYWMHMARRDLVQQSPKSDGRATGVFA"
     gene            complement(421..1080)
                     /locus_tag="VFX46_07380"
     CDS             complement(421..1080)
                     /locus_tag="VFX46_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX5600440.1"
                     /translation="MLRTCVVGVLLAAGTLLAWGCSRTPAFIAEYEPWRDEEERACLA
                     SGYVQRTPYLLTRASLGGPSVCGALQPFEMTAAAGGRVSLKPAAMLRCPMIPAVEWWI
                     EQVVEPAAARHFGVPLAEVKVAASYGCRPMNNKWGARLSEHGHANALDVSGFVLADGR
                     RIDVKSGWWGDPRERSFLRAVHRGACGAFTTVLGPDYDSLHRDHFHLDLARRRDGRVC
                     K"
     gene            complement(1392..2213)
                     /locus_tag="VFX46_07385"
     CDS             complement(1392..2213)
                     /locus_tag="VFX46_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HEX5600441.1"
                     /translation="MQWVSRISAAVAALLIVLQAGDAARADTTVDLELVLAVDVSLSM
                     DLEEQRLQRDGYIEALRDPQVISAIQSGLHGRIAITYVEWAGPYSQTTVLPWTIIDGK
                     ESAERVAEQLAQAPISRRRMTSISSALDYAGRQFELSPHRGIRRVIDISGDGPNNSGG
                     PVTPVRDELVKRGIIINGLPVILRPSQSSWFDISYLDRYYAECVIGGPGAFMIPIRDK
                     SEFATATRQKLILEISGAEPPPQLILAQYPSEDEKVDCLIGETLWRRYMDGPFRN"
     gene            complement(2284..3036)
                     /locus_tag="VFX46_07390"
     CDS             complement(2284..3036)
                     /locus_tag="VFX46_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600442.1"
                     /translation="MQSFLSDGVEIAFLDEGAGAPVLLIHGFASNTVVNWVDTGWVRF
                     LTSNGFRVISIDNRGHGRSQKLYRVEDYGAPQMAEDARRLLDHLGIPRADVMGYSMGA
                     RITAFLALAHPDRVRSCIFGGLGINMVRGMAGTGPIAHALEAESIDQVTNPTARTFRA
                     FAEQTKSDLKALATCIRSSRDPITAEDVGRIACPVLVVVGSDDVVGGSASALAALIPR
                     AEAVEIPDRDHMKTVGDRRYKEAVLDFLRRRP"
     gene            3153..>3359
                     /locus_tag="VFX46_07395"
     CDS             3153..>3359
                     /locus_tag="VFX46_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEX5600443.1"
                     /translation="MAEKIVPHFANDKGVEKIFIGVKEFKCMGARPPFDHPHVFLDMG
                     ADHQVICPYCSTLYVYDPRLNPDES"
BASE COUNT          670 a         1056 c         1061 g          572 t
ORIGIN      
        1 gcaaagacgc ccgtcgcccg cccatcggac ttggggcttt gctgcacaag atcgcgccgc
       61 gccatgtgca tccagtagag gacttcggcg cgctcgtagc gttcgagccc taccagccct
      121 ggccgccagg gctcgtcgat ctcgatccgg cagacggggc cttcgggcga cccctgccga
      181 gggcagtcgt cacggctggc gaacggcgtg cgtatgcggc cgatgaagac aaggcgcgcg
      241 tccgtccgct ccggcagcgg cacctccaac tcgcctgcgc gcgtggcgtc ctgatccacg
      301 gctgacctca cgcgattggt gatgccatca agatgagaga gccgcaccgc gatcgcaact
      361 gggcagatgg actcagcgat gcatccaacg ctgtcggggt gagtagagcc agaggtcgaa
      421 ttacttacaa actctgccgt ccctgcggcg cgcaaggtcg aggtggaagt ggtcgcgatg
      481 gagggaatcg taatcgggcc cgagcacggt cgtgaacgcg ccgcaagcgc ctcgatggac
      541 ggctcgcaag aaggagcgct cgcgaggatc accccaccat cccgacttca cgtcgatgcg
      601 gcgcccgtcg gccaacacaa agcccgagac gtcgagcgcg ttggcgtggc cgtgctccga
      661 taatcgggcg ccccatttgt tgttcatggg gcggcagccg taagaagcgg caactttgac
      721 ttcggcgagg ggcacgccga agtgtctcgc cgcggcgggc tcgaccactt gctcgatcca
      781 ccactcgacc gctgggatca ttgggcagcg gagcatcgcg gcaggcttta agctgacccg
      841 gccgccagcc gcggcggtca tctcgaacgg ctgcagcgcg ccgcagacgc tcggtccacc
      901 aagcgaagcg cgcgtcagta agtaaggtgt gcgctgaacg tatccggagg ccaggcaggc
      961 ccgctcttcc tcgtcccgcc agggctcata ctcagcaatg aacgcgggtg tacggctgca
     1021 tccccaggca aggagagtcc cagccgcaag caacacgcca acaacgcagg tacgcaacat
     1081 ggaaagggaa gctacgtgga gcaaggttaa cggaaggtga gcagcctcgt caggctgaca
     1141 ctgccgttag ccgactcggc ggcacgaatg tgccggccgc acggcttttg ccgatcggaa
     1201 cttggccgca attgtggaga gattcgtcgc tagaaactcc gtcctgcacc cttgcgagga
     1261 aggccagata tttcggagat cgtattcgga gagcgatttc aggcctgcgg atcgcgactc
     1321 gcggcaagtc cgaccatgta ggcggcccaa gctctcaggc cgaggttctc aatagcgaaa
     1381 aacagaaaaa gctaattccg gaacgggccg tccatgtatc gccgccaaag cgtttcgccg
     1441 atcaggcagt ccactttctc atcctcggat gggtattgag ccaaaatgag ctgtggcggc
     1501 ggctctgcgc cggagatttc gagaatgagt ttctggcgag tcgccgtcgc gaactccgac
     1561 ttgtcgcgga tcgggatcat gaaggcaccg ggtccgccga tgacgcactc ggcgtagtag
     1621 cggtcgagat agctgatgtc gaaccagctc gactggctgg gtctgaggat gaccggcaga
     1681 ccattgatga tgatgccccg cttgacgagt tcatcgcgca ccggcgtaac aggccccccg
     1741 gagttattcg gcccatcccc tgagatgtcg atgacgcggc gaatgccgcg gtgtgggctc
     1801 agctcgaatt gccgcccagc ataatccaga gccgatgaaa tcgacgtcat ccgcctccgg
     1861 gagatcggcg cttgcgccag ctgctcggcg acccgctcgg ccgactcctt cccgtcgatg
     1921 atcgtccaag gcagcaccgt cgtctgcgag tacggccccg cccactccac gtacgtgatg
     1981 gctatgcgtc catggagccc ggactgtatg gcggaaatga cctgaggatc ccggagcgcc
     2041 tcgatgtagc cgtccctttg taaacgctgc tcctcgaggt ccatcgaaag agagacatcg
     2101 acggcgagaa ctagctcgag atcgacggtc gtatcggcgc gggccgcatc gccggcctgc
     2161 agcacgataa gcagcgcggc aacggccgcg ctgatacgcg agacccactg catgagggca
     2221 acactgccat atcgcgtagt agttgcaaag gcttaggctg cccggtcaat taattgtgaa
     2281 ggtttacggc cggcggcgca gaaaatcgag caccgcctcc ttgtagcgcc ggtcaccaac
     2341 ggtcttcatg tgatcgcggt ccgggatctc gacagcttcc gcccgcggga taagtgcggc
     2401 gagtgcggag gccgaacctc cgacgacatc gtcgctgccg acgacgacca ggaccgggca
     2461 ggcaatgcgc ccgacatcct ccgccgtgat cgggtcccga gacgaacgga tgcaggtggc
     2521 gagcgccttg agatcgcttt tcgtctgctc ggcaaaggcg cggaaggtgc gggcggtggg
     2581 gttcgtcacc tgatcgatgc tctcggcctc caaggcatgg gcgatagggc cagtacccgc
     2641 catgccacga accatgttga tcccgagacc gccgaatatg cagcttctca cgcgatccgg
     2701 gtgagccagc gcgaggaaag ctgtgatccg cgcgcccatg gagtagccca tgacgtccgc
     2761 tcgggggatg ccgaggtgat cgagcagccg gcgcgcatct tccgccatct gcggggcacc
     2821 gtaatcttcg acgcgataga gcttctggct gcggccatgc ccgcgattgt cgatcgaaat
     2881 cacgcggaag ccgttgctgg tgaggaaccg aacccagccc gtgtcgaccc agttgaccac
     2941 ggtgttcgag gcgaaaccgt ggatcagcag gaccggggcc ccagccccct catcgagaaa
     3001 ggcgatctca accccatcgg acagaaagct ttgcacggca gcctccgcat catcctggcg
     3061 cttgcgacag aggcttccct accattcggg ctgccggccg ggtatggtcg gcaccgcgag
     3121 gctaggtaac caatggtgca agggcaagcc ggatggccga gaaaatcgtc cctcatttcg
     3181 caaacgacaa gggcgtcgag aaaattttca ttggcgtcaa ggagttcaaa tgcatgggtg
     3241 cacggccgcc cttcgaccac ccacacgtct tcttggacat gggggccgac caccaggtca
     3301 tctgcccgta ctgctcgacc ctttacgtct acgacccgcg actgaaccct gacgaatca
//
LOCUS       DASUMG010000450         3266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_942425, whole genome shotgun sequence.
ACCESSION   DASUMG010000450 DASUMG010000000
VERSION     DASUMG010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942425"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VFX46_07400"
     CDS             <1..806
                     /locus_tag="VFX46_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX5600444.1"
                     /translation="VEEDASLSVEQNSDTHQLVLLGQGEMHLRVALERLNRKYGVAVE
                     KQRRLVPYKETIRKGTTVRGRHKKQSGGHGQFGDVVLDIKPLPRGSGFQFSETITGGV
                     VPRQFIPSVEIGVRDYLNQGPLGFPVVDVAVTLTDGSYHSVDSSDMAFRQAGRIAMAE
                     GMPKCNPVLLEPIMAVEITVPSDATAKINGLVSQRRGQILGFDAREGWPGWDVVQVQI
                     PAAEMDDLIVELRSATAGVGSFTAKFDHLAELTGRLADQVLASHKAAAE"
     gene            912..1592
                     /locus_tag="VFX46_07405"
     CDS             912..1592
                     /locus_tag="VFX46_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX5600445.1"
                     /translation="MSSSSLSPSLTLTAPEFRMLARQRLQESPSEAIFDPRTGRSWGR
                     SDFDLNPELLADLAAMEPPRPAAVLVPVVAREQLTVLLTVRTDHLPTHAGQVAFPGGK
                     VDRRVDSGPLDTALREAEEEIGLEARYIEPLGFLDSYRSGTGFHIMPLVALVEPTFTL
                     RPDPAEVADVFEVPLSFLMDTANHQRHSREWRGRLRYFYAIPFGNRYIWGATAGMIRN
                     LHERLFGP"
     gene            1589..1858
                     /locus_tag="VFX46_07410"
     CDS             1589..1858
                     /locus_tag="VFX46_07410"
                     /inference="COORDINATES: protein motif:HMM:NF040327.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HEX5600446.1"
                     /translation="MIRLIIENTLLFLLPTVIYVAYVLLKRRIEGNSNVNVFDDAPLI
                     WLVAAGAALVMITLITFGSISGGKPGQVYHPPVYRDGKIIPGHYK"
     gene            1869..3137
                     /locus_tag="VFX46_07415"
     CDS             1869..3137
                     /locus_tag="VFX46_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX5600447.1"
                     /translation="MPAEGGERLPCLAGAEWLKRPQTQAVLRAIEAGGYPARAVGGVV
                     RNALLGAPVADVDVATPAPPGEVIRLAEIAGLKAIPTGLKHGTVTIIVDHTPFEVTTL
                     RKDVETFGRHARVVFTQDWAADARRRDFTINALYCDADGRVYDPLNGYPDILARRVRF
                     IGDAAERIREDYLRILRFFRLTAVYGAGPPDPDGLAAAVRERAGLARLSSERIRQELV
                     QLLAASRAPELIEVMRDYGLLTMVLPVAPRPGLLHRLAAIEQSLALQPDVMLRLAVLG
                     VEVVEDAIKLRDHLRLSNAEFDTLVLAANAPREGVHPQSSEQEAKVSLYRKGPSDMRK
                     QLLINWARSGISPDHPNARVLYAFPDRWDPPRFPVSGADVLEAGVPSGPRVGDILDEL
                     ERRWIEADFAWDRERLLSELADVISEADQA"
BASE COUNT          598 a          964 c         1043 g          661 t
ORIGIN      
        1 tggtcgagga ggacgcctcg ctttccgtgg aacagaattc ggatacgcat cagctcgtcc
       61 tacttggaca gggtgagatg caccttcggg tggcactcga gcggctcaac cggaagtacg
      121 gcgtagctgt cgagaagcag cggcggctcg ttccctacaa ggagacgatc cgcaagggca
      181 cgaccgtgcg cggccgccac aaaaagcaat ccggcggaca cggccagttc ggcgatgtgg
      241 tgctcgacat caagccgttg ccgcgcggtt cgggtttcca attttccgaa accatcaccg
      301 gcggcgtcgt acctcgccag ttcattccct ccgtggagat tggtgtgcgg gactatctca
      361 accagggacc gctcggcttt ccagtggtcg acgtggctgt gacgctgaca gacggcagct
      421 atcactcggt cgactcgtcc gacatggcct tccgccaagc tggccgcatc gcgatggctg
      481 aaggcatgcc gaagtgcaat cccgtgttgc tcgagccgat catggcagtc gagatcaccg
      541 ttccttcgga tgcgaccgcg aagatcaatg gtctcgtctc tcagcgccgc ggtcagatcc
      601 tcggcttcga tgctcgcgag ggttggccag gctgggatgt tgtgcaggtg cagatcccag
      661 cggcggagat ggacgacttg atcgtcgagc tgcgctcggc gaccgctggc gttggatcgt
      721 tcacggcgaa gttcgaccat ctggctgagc tgaccggccg gcttgccgat caggtgcttg
      781 cgagccacaa ggcagcggcg gaataggagg ggaacacaga gatcgaagaa gatgctggga
      841 cctgcgggcg gcgtcgcagg gatgctgctc ttcttgccaa tttgcgcgtg agtgagcatg
      901 gtgtcccaac catgtcctcg tcgtctctca gcccgtccct tacgctcact gcacccgagt
      961 tccgaatgct tgcccgtcag cggctccagg agtcgccgag cgaggcgatc ttcgacccgc
     1021 gaacgggtcg gagctggggc cgtagcgact tcgacctcaa tccggagctg ctggcagatc
     1081 ttgccgccat ggagccgccc cggccggcgg cggtgttggt ccccgttgtc gcccgtgagc
     1141 agttaacggt cttgctgacg gttcgcacgg atcatctccc cacccatgcc ggccaagtcg
     1201 cgtttcccgg tggaaaggtc gataggaggg tcgactccgg tcccctcgac acggcgttgc
     1261 gggaggcaga ggaggagatc gggctcgagg ctcgctatat tgagccttta ggttttctcg
     1321 acagctatcg ttcgggaaca ggctttcaca tcatgccgct cgtggctctg gtcgagccga
     1381 ctttcacgct caggcctgat ccggccgagg tggccgatgt tttcgaggtg ccgctatcct
     1441 ttctcatgga tacggcaaac caccaacggc actcgaggga gtggcgcggc cgattgcggt
     1501 acttctatgc gattccattc ggcaatcgct atatctgggg agcgactgcc ggaatgatta
     1561 ggaacttaca tgaaaggctc ttcgggccat gatccgtctc attatcgaga acacgcttct
     1621 gttcctgttg ccgacggtca tctatgtcgc ttacgtgctg ctgaaacggc gaatagaggg
     1681 gaacagcaac gtcaacgttt tcgacgacgc accgctcatt tggctcgtgg ccgcaggagc
     1741 cgctcttgtc atgattacgc tgattacttt cggctcgatc tcgggcggaa aaccgggaca
     1801 agtttaccat ccgcccgttt atcgggacgg caagatcatt cctggccact acaagtgaga
     1861 aggcattgat gcccgcagaa ggcggcgagc ggctgccatg tctggcaggt gccgagtggc
     1921 tcaagcggcc gcaaacgcag gccgtgctcc gggcgataga ggcgggcggt tatccagcac
     1981 gcgcagtcgg aggtgtcgtc cgcaatgcgc tgcttggggc gccagttgcc gatgttgatg
     2041 tcgccactcc ggccccgccg ggagaggtga tccgtcttgc cgagatagcg gggctcaagg
     2101 cgattcccac cgggctaaag cacggcaccg tcaccattat tgtcgaccac actccgttcg
     2161 aggtgaccac cctgagaaaa gatgtggaga cattcggccg ccacgccagg gtcgtcttca
     2221 cgcaggattg ggccgctgac gcacgccgcc gggatttcac catcaacgct ctctactgcg
     2281 atgccgacgg cagggtgtac gatcccttaa acggctatcc ggatattctg gctcggcgcg
     2341 tgcgtttcat cggcgatgcc gcggagcgta tcagggagga ctatctccga atactgcgtt
     2401 tcttccgtct gaccgctgtc tatggcgccg gccctccgga cccggacgga ttggcagccg
     2461 cggtgcgtga acgcgcaggc ttggcgcggc tgtcgagcga gcgcatccgt caggagctgg
     2521 tgcaactgct ggcagcttcc cgcgcacccg agctcatcga ggtcatgcgg gattatggtc
     2581 ttctgaccat ggtgcttccg gttgccccgc gcccggggct gcttcaccgg ctcgctgcga
     2641 tcgagcaaag tctcgcgctg caaccggatg tgatgcttcg gctggcagtg ctcggagtgg
     2701 aggtggtgga ggacgcaatc aaattgcggg accacctgcg gctttcgaat gccgagttcg
     2761 atacgctcgt gctcgcagcc aatgcaccgc gcgaaggggt ccatccacag tctagcgagc
     2821 aagaggccaa agtctcgctt taccgcaaag gcccgagtga catgcgcaag cagttgctga
     2881 tcaactgggc gcggtcaggc atctctccag atcatccgaa tgcgcgcgtg ttgtatgcgt
     2941 ttccggaccg atgggaccca ccaaggtttc ccgtaagcgg cgctgatgta cttgaagcag
     3001 gggttccgtc cggaccgcga gtgggggaca ttctcgacga actcgagcgc cgctggatcg
     3061 aagcggactt cgcctgggat cgcgaaaggc tcctgtccga gctggcggac gtgatttctg
     3121 aggcggacca agcctaagtc cgctgagctg tcggccgata acttgaggtg accagagcgc
     3181 acgcaaatca aagcggcggt cagagcatga ccgccgctgg acgaaaaatg gatggcttct
     3241 cagttcttga ttgcatctga aggctt
//
LOCUS       DASUMG010000451         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_220548, whole genome shotgun sequence.
ACCESSION   DASUMG010000451 DASUMG010000000
VERSION     DASUMG010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220548"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..2404
                     /gene="purL"
                     /locus_tag="VFX46_07420"
     CDS             167..2404
                     /gene="purL"
                     /locus_tag="VFX46_07420"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX5600448.1"
                     /translation="MADTVGNAAQTPDESQPWAELGLKDDEYARIKEILGRRPTSSEL
                     AMYSVMWSEHCSYKSSKVHLRQFLDIPPETPTGKLLAGIGQNAGVIDIGDGLAVVFKM
                     ESHNHPSYIEPYQGAATGVGGILRDVFTMGARPIACLNALSFGDPKHPKTRHLVSGVV
                     AGVGGYGNSFGVPTVGGEVRFHRRYDGNCLVNAMAVGIAEKDKIFYAKATGVGMPIVY
                     LGSKTGRDGIHGATMASAEFDENSEEKRPTVQVGDPFTEKLLIEACLELMAEDAIIAI
                     QDMGAAGLTSSTSEMADKGGVGIELNLDNVPQREEGMTAYEMMLSESQERMLMVLKPG
                     AEAKAEAIFRKWGLDFAVIGQTTDTGRMIVKHKGRVEADIPVSKLANSAPVYERPWVP
                     TEPPQVIRPEDVTAPNTNLGALKTMMGHPQLCSRRWIWEQYDYMVMGHTVQRPGGDAA
                     IVRIPGTTKGIAMACDVTPRYVAADPDMGTRQAVVENWRNLTAVGADPLAITDNMNFG
                     NPERPEIMGQFVQSVKGMAEACRALDYPVVSGNVSLYNETNGVGIPPTPAIGGVGLVP
                     DLTKIADLALKAEGNVLVLIGREAGHLGQSLYQQIIAGRLEGAPPPVDFADEIKAGKL
                     IRSLIREAGVRTVHDVSDGGLLVAVAEMALAGDLGVELVAYEGALPTHAIWFGEDQGR
                     YIVEVSPDQAEDVIERARLLALPARIIGRVGGDELRLPGEAPLKLAELRDIHESWLPR
                     YMSGA"
     gene            2582..2818
                     /locus_tag="VFX46_07425"
     CDS             2582..2818
                     /locus_tag="VFX46_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEX5600449.1"
                     /translation="MPMDAKEIERLIKESIPDAEVTIRDLAGDGDHYAAHVVSSAFVG
                     KSRVQQHQMVYQALGGRMGDALHALALTTSPRKE"
     gene            2916..>3238
                     /gene="grxD"
                     /locus_tag="VFX46_07430"
     CDS             2916..>3238
                     /gene="grxD"
                     /locus_tag="VFX46_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX5600450.1"
                     /translation="MTEQQAVHDWIAKTIAANDVVLFMKGTSQFPQCGFSAQVAQILN
                     HLGVDYKDVNVLEDMGIREGIKTFSNWPTIPQLYVKGEFIGGCDIVREMFQAGELQSL
                     LAEKGV"
BASE COUNT          617 a         1007 c         1022 g          592 t
ORIGIN      
        1 gttgtcgcca tcgtgggcac catgctgaac gacggcggga tcttcgtctg gctcacggtg
       61 acggcacagg taaccacagt cgcgggctgg ttcttcctcg actgggcaga acgcaacgat
      121 tggactgtca gccccgtaag agccgaacgc cggtaacctg gccgccgtgg cagacactgt
      181 tgggaacgct gctcaaaccc ctgatgagtc tcagccatgg gctgagctgg ggctgaagga
      241 cgacgagtac gcccggatca aggagatcct gggccgtcgg ccgacttcat ctgagctcgc
      301 catgtactcg gtcatgtggt ccgagcactg ctcgtacaag tcgtccaagg tgcatctccg
      361 ccagttcctc gacataccgc cggagacccc taccggcaag ctgctcgccg gcatcgggca
      421 gaacgccggc gtgatcgaca tcggcgatgg gcttgccgtg gtgttcaaga tggagagcca
      481 caaccacccg agctacatcg agccctatca gggcgcggcg accggtgtcg gcggcatcct
      541 gcgcgacgtc tttaccatgg gcgcgcggcc gatcgcgtgc ctcaacgcgc tctcgttcgg
      601 cgatccgaag catccgaaaa cgcggcatct ggtgtcgggc gtcgtggcag gtgtcggcgg
      661 ctacggcaat tcgttcggcg tgccgacggt cggcggcgag gtgcgctttc atcgccgcta
      721 cgacggcaat tgcctggtca acgccatggc ggtcggcatc gccgagaagg acaagatttt
      781 ttatgccaag gcgaccggcg tcggcatgcc catcgtctat ctcggctcga agaccgggcg
      841 cgacggcatc cacggcgcga ccatggcctc cgccgagttc gacgagaact ccgaggaaaa
      901 gcggcccact gttcaagtcg gtgacccgtt caccgaaaag ttgctgatcg aagcgtgtct
      961 cgaactgatg gccgaggacg ccatcattgc catccaggat atgggcgccg cgggccttac
     1021 ctcctccacc tccgagatgg ccgacaaggg cggcgtcggc atcgagctta atctcgacaa
     1081 cgtcccccag cgcgaagaag gcatgacggc ctacgagatg atgctttctg aaagccagga
     1141 gcgcatgctc atggtgctga agccgggggc cgaggccaaa gcggaggcca ttttccgcaa
     1201 gtgggggctc gatttcgcag tcatcggcca gaccaccgac acgggccgaa tgatcgtcaa
     1261 gcacaagggt cgcgtcgagg cggatattcc cgtttccaag ctcgccaatt cggcaccggt
     1321 ttacgaacgg ccgtgggtgc cgacggagcc gccgcaggtc atccggcccg aggatgtcac
     1381 agcgcccaac accaatctcg gtgcgctcaa gactatgatg ggccacccgc agctctgctc
     1441 ccgccgctgg atctgggagc agtatgacta catggtcatg ggccacacgg tgcagcggcc
     1501 aggaggtgat gccgccatcg tgcgcatccc cggcaccacc aagggcatcg ccatggcctg
     1561 cgatgtcacc ccacgctacg tggccgctga tcccgacatg ggcacgcggc aggccgtggt
     1621 cgaaaactgg cggaacctga cggctgtcgg tgccgatccg ctcgccatca cagacaacat
     1681 gaacttcggc aaccctgagc ggccggagat catgggccag ttcgtccagt cggtgaaagg
     1741 catggccgag gcctgccgag cgctggacta tcctgtcgtg tcgggcaacg tctcgcttta
     1801 taacgagacc aacggtgtag gcattccgcc gacgcccgcc attggcggcg ttggactcgt
     1861 tcctgatctc accaagatcg ccgacctcgc gctcaaggct gaaggcaatg ttctcgtcct
     1921 catcggccgc gaagcggggc acctcggaca atcgctctac cagcagatca tcgccggtcg
     1981 ccttgaaggc gccccgccgc cggtcgattt cgccgatgag atcaaggcgg gcaagctgat
     2041 ccgctcgctc atccgcgagg ctggcgtgcg taccgtccat gatgtcagcg acggtgggct
     2101 cctcgttgcg gtcgctgaga tggcgttggc cggtgatctg ggtgtcgagc tggtcgccta
     2161 cgagggcgca ttgcctaccc acgcgatctg gttcggtgag gatcagggcc ggtacatcgt
     2221 cgaagtgtcg cccgaccagg ccgaggacgt catcgagcgt gcccgtctgc tcgccctccc
     2281 ggcccgcatc ataggaaggg tcggcgggga cgagcttcgt ctacctggcg aagcacctct
     2341 gaaattggcc gagctgcgcg acattcacga aagctggctt ccccgataca tgagcggtgc
     2401 gtaaccgctt gtatcagcgg gcgttcttgc aatgttcgcc gccgtgctct aattgtcgaa
     2461 taggcaagcg gcaggcattg tggccgcctc ttcccgcgct tatgttgcgg atgcgagatt
     2521 ctaggaagcg cacgggtgga cgcgctcgaa cttaattggc cacctgggga gacgttttct
     2581 catgccgatg gacgcgaaag aaattgagcg gctgataaag gagagcatcc cggatgccga
     2641 ggtcaccatt cgggacctgg ccggcgatgg cgatcactat gcagcccacg tcgtttcatc
     2701 cgctttcgtc ggaaagtcgc gagttcagca gcatcaaatg gtctaccagg cgctcggcgg
     2761 ccgcatgggg gatgctctcc acgcgctggc gctgaccact tcgccgcgca aggagtgact
     2821 tagggagcat tgactcccac cagcacgcgc ccccatgttc agtccactag ggccagggcg
     2881 cctttgaacc gcctcatcga gccaagggga tttccatgac agagcagcag gccgttcacg
     2941 actggatcgc gaagacgatt gcggccaatg acgtcgtgct tttcatgaag ggcacgtcgc
     3001 aatttccgca gtgcggtttc tccgcccaag tggcgcagat cctcaaccac ctcggcgtcg
     3061 actacaaaga cgtgaacgtg ctcgaggaca tgggaattcg tgagggcatc aagaccttca
     3121 gcaactggcc cacgatcccg cagctgtacg tcaagggcga gttcatcggt ggctgcgaca
     3181 tcgtccgcga gatgttccag gctggcgaat tgcagagcct gctggctgaa aagggcgt
//
LOCUS       DASUMG010000452         2188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_460396, whole genome shotgun sequence.
ACCESSION   DASUMG010000452 DASUMG010000000
VERSION     DASUMG010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460396"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VFX46_07435"
     CDS             <1..228
                     /locus_tag="VFX46_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600451.1"
                     /translation="TTKYENPHVTLTVEAQDKTWTLTLAPVSRMRNRGATEELVAPGT
                     VITAVGYVSKHDPDEMRAEHITVDGKTIQMR"
     gene            229..720
                     /locus_tag="VFX46_07440"
     CDS             229..720
                     /locus_tag="VFX46_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HEX5600452.1"
                     /translation="MALEAPAILVALEQSALGTAIRQSVWIYPAANTVHILGLTLLAA
                     SVAVMDLRLLGAFRETVPAAVIVPARRLAMLGLTIMVLSGLVLFTAEATHVAMNPVFQ
                     IKMGLIALAILNALIVQRTLPAALIKTPAMTPLPMRFRIAAAVSLVLWFCVAAAGRLI
                     AYF"
     gene            complement(740..1342)
                     /locus_tag="VFX46_07445"
     CDS             complement(740..1342)
                     /locus_tag="VFX46_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX5600453.1"
                     /translation="MQQTVLAAMVVLMPRYLIAYASREGQTEKIAHHIAHRLENDGAL
                     VRLIDLKAHETEAGADDCDAMIIAGSVHIGQFDPELSSFVMRHGEALRRCPSAFLPVS
                     LSAASHEESERSAIDEIAQRFVAEHGWKPDVVYHVAGAIHDRELNPIERVILHAIADS
                     KGVERHPSGDTELTDWRGLDQFVREFEALVQSDASRAVGE"
     gene            complement(1411..>2188)
                     /gene="fetB"
                     /locus_tag="VFX46_07450"
     CDS             complement(1411..>2188)
                     /gene="fetB"
                     /locus_tag="VFX46_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HEX5600454.1"
                     /translation="WLDLAAAAVLIVFNGVLSWALRLGLERSIAIATVRMVAQLGAIG
                     FVLKFVFEQTSPLLTAALALVMVAMAGHEALARQESRVGGWMAYGIGTGTLFFVGLLA
                     TLYTMIVVMGPEPWYAPRYLLPILGMILGNTLTGVALVMNTIVQAAKRDRAAIEARLA
                     LGETRFEATGSVIVHAVKTGLIPILNAMAATGVVTLPGMMTGQILAGIDPVEAAKYQV
                     MIMFVIAAATALGVMLAAIGGAWLITDSRHRLRVGMLGLV"
BASE COUNT          430 a          708 c          640 g          410 t
ORIGIN      
        1 acgaccaaat acgagaaccc gcacgtcacg ctcaccgtcg aggctcagga caagacttgg
       61 acgctgacgc ttgccccggt atcgcgcatg cggaaccgcg gtgcgaccga ggagttggtg
      121 gcccccggca cggtcatcac cgcagtgggc tatgtctcga agcacgatcc cgacgagatg
      181 cgggccgagc acatcaccgt cgacggcaaa acaatccaga tgcgttgaat ggctctcgaa
      241 gcccccgcaa ttcttgtcgc gttggagcaa tcggctctgg ggacggccat ccggcaatcg
      301 gtctggatct atcctgcagc aaataccgtt cacatccttg gactaaccct cctggcagcc
      361 tcggtcgcgg tcatggatct gcggctgctc ggtgcgttca gagagaccgt tccagctgcg
      421 gtaatcgtcc cggcgcgccg tctcgccatg ctcgggctga ccatcatggt gttgtcgggc
      481 cttgtgctgt ttacggccga ggcgacccac gtggccatga acccggtctt tcagatcaag
      541 atgggcctga tcgcgctcgc gatcctgaat gctctcattg ttcagaggac gctacccgcg
      601 gcgttgatca agacgccggc gatgacacct ttaccgatgc gatttcgaat tgcggccgcc
      661 gtttctctgg tgctgtggtt ctgcgtggca gctgccggtc ggctcattgc gtacttctga
      721 ccgtgcttgc gacgcccctc tactcaccca ccgcgcggga tgcatctgac tgcaccagcg
      781 cctcaaactc acgaacgaac tgatcaagcc cacgccaatc ggtcagctcc gtatcgccgg
      841 atgggtggcg ctcgacgcct ttggaatcgg cgatggcgtg aaggatcacc cgctctatcg
      901 gattgagctc tcggtcgtgg atggcgccag ccacgtgata gacgacatcc ggcttccacc
      961 cgtgctcagc cacgaaacgt tgcgcaattt cgtcgattgc ggaccgctcg ctttcctcgt
     1021 gagacgctgc cgacaggctc accggaagaa aagcggaagg gcagcgccgc aacgcctcac
     1081 catgacgcat gacgaagctt gataactccg gatcgaattg gccgatatgg accgagccag
     1141 cgatgatcat cgcatcgcaa tcgtcggctc cggcctccgt ttcatgagcc ttgaggtcga
     1201 tcagccgaac cagcgcgcca tcattctcga gccgatgtgc gatgtgatgg gcgatctttt
     1261 ccgtttgccc ctcgcgcgag gcgtaggcga tgagataacg cggcatgagc accaccattg
     1321 cagcaaggac agtctgctgc aacacactgg attgtcattg cttcttatca acctaggcgc
     1381 ttgaacggtt cccgcccacg atttcagtga ctagaccaag ccgagcattc ccacgcgcaa
     1441 acgatggcgc gagtcggtga tcagccaggc gccgccgatg gccgccagca tcacgcccag
     1501 agcggtggcc gccgcaatca cgaacatgat catcacctga tacttggccg cctcaaccgg
     1561 atcgatgccg gcgagaatct ggccggtcat cattccgggc agcgtgacga cgcctgttgc
     1621 agccatcgcg ttcaggattg gaatgaggcc cgttttcacg gcatgaacga tgacgctccc
     1681 cgtcgcctcg aaacgcgttt cgcccaatgc caaacgcgcc tcgatcgctg cgcggtcgcg
     1741 cttggcagcc tgaacgatcg tgttcatgac gagggcgacg ccggtcagcg tgttgccgag
     1801 tatcattccg aggatcggca gcaagtaccg cggcgcgtac cacggctcag ggcccatgac
     1861 gacgatcatg gtgtagagcg tcgccagcaa tccgacgaag aacagtgtac cggtgccgat
     1921 gccgtaggcc atccagccgc cgacacgcga ttcttgccgt gccagcgctt catgccccgc
     1981 cattgcgacc atcacaagcg ccagcgccgc cgtcagcagc ggtgaggtct gttcgaatac
     2041 gaacttcagg acgaagccga tcgcgccgag ctgcgcgacc atccggaccg ttgcgatcgc
     2101 gatgctgcgc tcgagcccaa gccgcagcgc ccaggacaat accccgttga agacgatgag
     2161 aacggcggcc gccgcaaggt cgagccac
//
LOCUS       DASUMG010000453         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_460414, whole genome shotgun sequence.
ACCESSION   DASUMG010000453 DASUMG010000000
VERSION     DASUMG010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460414"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFX46_07455"
     CDS             <1..333
                     /locus_tag="VFX46_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600455.1"
                     /translation="RIDVDYRFADGKGNITLSALYNGRMADRGLRTDFVTETGFLIPK
                     SEPVTLDDYWLVTAAASYKLEPGVELFGRVENLFDSDYEEIFGYNTPGIAAYAGVRFT
                     LGGSGIAD"
     gene            442..1521
                     /locus_tag="VFX46_07460"
     CDS             442..1521
                     /locus_tag="VFX46_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5600456.1"
                     /translation="MGKQSRRRINLALQGGGAHGAFTWGVLDELLRDETLDLGWISGT
                     SAGAVNAAAVASGLANGGRDEARATLRSVWEAVEAAGIPDLLRFNPFLLGFSRAAPVS
                     TVANLFSPYEFNPLGFDPLRRLLAEHIDFERIREAAPVELLIAATDVSTGRARLFRRA
                     ELTIDAVLASACLPTLHHAVVIDGRAYWDGGFSANPDLVTLASESPVEDTLLVQLTPL
                     QHSDIPRTATEIAGRVNAITFNQPLLRDIREIVAAQETRIGWFSGRENRAARLKRHRF
                     HLIEASRYTAGLRAGSALKPDKALLSYLYEAGKTEARKWLKEHSSKVGRTSTVDLCAH
                     FLESAPPESSEVADESRRQAPAVGS"
     gene            complement(1548..>2050)
                     /locus_tag="VFX46_07465"
     CDS             complement(1548..>2050)
                     /locus_tag="VFX46_07465"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX5600457.1"
                     /translation="ERLVEIRPRHGARILPVSVDDMREIYEMMTELEAFAVRKLAERG
                     LSRSELRQLEQVLADMDEALSRDDLMSWARHDETFHALLVDLTGNSRLSQVVATFRDQ
                     AHRARMQTLKLRPKPVDSNRDHAAVVEAIRQRDPETAAATHRQHRQRAGAQLLMLLAR
                     CGSEGL"
BASE COUNT          367 a          636 c          660 g          387 t
ORIGIN      
        1 cgcatagatg tcgactatag gtttgccgac ggaaagggca acatcaccct atcggctcta
       61 tataatggcc gaatggccga tcgcggactt cgcactgact tcgttacaga aactggcttt
      121 ctgattccca agtcggaacc cgtcaccctc gacgactact ggctcgtcac cgcagccgcc
      181 tcctacaagc ttgagcccgg cgtcgagctc ttcggacgcg tcgagaacct gttcgattcg
      241 gactacgagg agatcttcgg ctacaacacg ccgggtatcg ctgcctacgc aggtgtgagg
      301 ttcacgctcg gcgggagcgg catagcggat tgatcaccag atctgggagg cgcacggatc
      361 tccacctctt gttccggcag cctccgagct gccatgttcc gatgagagac gagcgagggc
      421 agcaggcggg gcagtcggaa ggtggggaag caaagtcggc ggcggatcaa tctggcgctg
      481 cagggcggag gcgcacatgg cgccttcact tggggcgtgc tcgatgagct gctgcgggac
      541 gaaaccctag acctgggttg gatcagcggt acgagcgccg gtgccgtcaa tgccgccgcg
      601 gtcgcatcgg gactcgccaa cggtggtcgt gacgaagccc gagcaacatt acggagtgtg
      661 tgggaagccg tcgaggcggc gggaattccc gatctgctgc gtttcaatcc gtttttgctc
      721 ggcttctctc gtgcggcccc ggtatccacg gtagccaacc tgttctcgcc ctacgagttc
      781 aatcccctcg gcttcgatcc gttgcggcgg ttactggcgg agcatatcga tttcgaacgg
      841 atccgagagg cggcgccggt ggagctcctc atcgcggcga ccgacgtgtc aacggggcgc
      901 gcacggttgt ttcgccgagc cgagcttacc atcgacgctg tcctggcgtc ggcttgcttg
      961 ccgacgctgc accatgcggt ggtgatcgac ggccgggctt actgggacgg cggtttttca
     1021 gccaacccgg atctcgtcac gctggcgtcg gagagcccgg ttgaggacac actcctcgtt
     1081 cagctcactc cgcttcagca ttccgatatc ccacgaacag caacggagat tgcaggccgc
     1141 gtcaacgcga tcacattcaa ccagccgctg ctgagggata tcagggaaat cgtggcggcg
     1201 caggagaccc gcatcggctg gttctccggt agggagaacc gcgcggcgcg cctcaaacgg
     1261 caccgcttcc atctcatcga ggcgagccgt tacaccgccg ggctccgggc tggcagtgcg
     1321 ctcaagcctg acaaggcgct gctctcgtat ctctacgaag cagggaagac cgaagcgagg
     1381 aaatggctga aggagcacag ctccaaggtc ggacgcacct cgacggttga tctgtgcgcg
     1441 cacttcctgg aatcggcgcc gccggaatca tccgaggttg ccgacgagag ccggcgacag
     1501 gctccggcag tcggctcctg aaagctggtc agtcggcgta catctcatta gagaccctcg
     1561 cttccgcaac gcgccagaag catcagcagc tgcgctccag ctctctggcg gtgctgccga
     1621 tgggtggcgg cggctgtttc cggatctctt tggcggatcg cctcgacgac ggcggcgtga
     1681 tcacggttcg agtcgacggg tttcggccgc agtttgagcg tctgcatgcg tgcgcggtgc
     1741 gcctgatcgc gaaaggtcgc gacgacctgg ctcagccgcg agttgcccgt gaggtcgacc
     1801 agcagtgcat gaaaggtctc gtcgtggcgc gcccagctca tgagatcgtc gcgcgacaac
     1861 gcctcgtcca tgtccgcaag gacctgctcg agctgacgga gctcgctgcg ggacaggccg
     1921 cgctctgcaa gcttgcgcac ggcaaaggcc tcgagctcgg tcatcatctc atagatttcg
     1981 cgcatgtcgt cgaccgacac gggcaggatc cgtgctccat gccgcggccg gatttcgacc
     2041 agccgttcat
//
LOCUS       DASUMG010000454         2496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_140656, whole genome shotgun sequence.
ACCESSION   DASUMG010000454 DASUMG010000000
VERSION     DASUMG010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140656"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VFX46_07470"
     CDS             complement(<1..604)
                     /locus_tag="VFX46_07470"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX5600458.1"
                     /translation="MREKRTEEDLIPPDARAVLRIDLDALRANWRKLNEASGRAECAA
                     VIKADAYGLGIEQICRALTEEGCRTFFVATLDEGRRIRAVQPGAVIYVLDGLLPGAAE
                     YYSGFDLRPALSSLEEIREWAAYCRARDTRLAAAIHIDTGMNRLGLPAKEVDELARNP
                     EVLNDFKVSLIMSHLACADTPASPMNAAQRETFERLTARLP"
     gene            complement(634..2145)
                     /locus_tag="VFX46_07475"
     CDS             complement(634..2145)
                     /locus_tag="VFX46_07475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX5600459.1"
                     /translation="MTKSALLPSDKLRAQAAAGEPLEFRQPPHNIEAEQALLGAILIN
                     NEAMDRVAGFLEPHHFYDPLHQQIYEVAGKLIQAGKQATPITLRTFFENAEPIDDDLT
                     VPQYLGRLAANATTIINAADYGRTIYDLAIRRALISIGEDLVNTAYDSPVDFPPEQQI
                     EEAENRLYELAEKGKYGQGFLTFGQALTQAIEMANAAYQREGHLSGLSTGLVDLDAKL
                     GGLQNSDLIILAGRPSMGKTALATNIAYNVAKAYRAEKQPDGTEKVVDGGVVGFFSLE
                     MSAEQLATRILSEQAEIPSEKIRRGMITEDEFRRLVRVSQEMAQIPLFIDQTGGISVA
                     QLAARARKLKRQHGLGLIVVDYLQLLTGSSRRAAEGRVQEVSEITTSLKALAKELNVP
                     ILALSQLSRAVEQREDKRPQLADLRESGSIEQDADVVLFVFREEYYVERRKPSESNVE
                     ELLKWQQEMAAVHGKAEIIIGKQRHGPTGTVELAFEASYTRFSNLARDYQLPE"
     gene            complement(2307..>2496)
                     /locus_tag="VFX46_07480"
     CDS             complement(2307..>2496)
                     /locus_tag="VFX46_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX5600460.1"
                     /translation="RMWEFYLAGSEAAFRYHGLIVFQIQMVKRIDALPITRDYMFEAE
                     QRLSQGAAPSKPARLAGE"
BASE COUNT          427 a          788 c          805 g          476 t
ORIGIN      
        1 gcggcaagcg tgccgtcagc cgctcgaaag tctcacgttg cgccgcgttc atcgggctcg
       61 ccggcgtgtc ggcgcacgcc aagtggctca tgatgagcga cactttgaag tcgttgagga
      121 cttcaggatt tcgtgccagc tcatccactt ccttggccgg caaccccagg cggttcatgc
      181 ccgtgtcgat atggatggca gccgcaagcc gggtgtcgcg cgcacggcag taggctgccc
      241 attcacggat ctcttccagg ctggaaaggg cgggccgcag atcgaagccc gagtagtact
      301 cggcagcgcc gggcagaagg ccatcgagga cgtaaataac agcgccgggt tgcacggccc
      361 ggatgcggcg gccctcgtcc agcgtggcaa cgaagaaggt tcggcatccc tcctcggtga
      421 gggcgcggca gatctgctcg atgccgaggc catacgcgtc cgccttgatg acggcggcgc
      481 actcggcccg tcctgaggct tcgttgagct tgcgccagtt cgcccgcaac gcgtcgagat
      541 cgatacgcag gacggcgcgg gcatccggtg ggatcaaatc ctcctcggtt cgtttttcgc
      601 gcacgccctt atccaccgaa ccgctcctac cctttactca ggaagctggt agtcccgtgc
      661 caagttgctg aaccgcgtgt agctcgcctc gaacgcgagc tccaccgtgc ccgtgggccc
      721 gtggcgctgc ttgccgatga tgatctcggc cttgccgtgc acagccgcca tctcctgctg
      781 ccacttgagc aactcttcta cgttcgactc ggacggcttg cgccgctcga cgtagtactc
      841 ctcgcgaaag acgaacaaca ccacgtccgc gtcttgctcg attgagccag actcgcgcaa
      901 gtcagcgagc tgcggccgct tgtcctcgcg ctgttccacc gcacgtgaca attgggatag
      961 cgcgagaatc ggcacattga gctccttggc caaggccttg aggctcgtgg tgatttccga
     1021 gacctcctga acgcgtccct cggcggcgcg gcgcgacgag ccggtgagca gctggagata
     1081 gtcgaccacg ataaggccga gcccgtgctg gcgtttgagc ttgcgggcgc gggcggcgag
     1141 ctgggcgacc gagatcccac ccgtctggtc gatgaacagc ggaatctggg ccatctcctg
     1201 gctgacccgg accagccgcc ggaattcatc ctcggtgatc attccgcggc ggatcttctc
     1261 ggacggaatc tccgcctgct cggagaggat gcgcgtggcg agctgctccg ccgacatctc
     1321 gagggagaag aagccgacca ctcctccatc caccaccttc tcggtgccgt ccggctgttt
     1381 ttcggcccgg taggccttgg ccacgttgta ggcgatgttg gtggcgagcg ccgtcttacc
     1441 catcgacggg cggcccgcaa gaatgatcaa atcggagttt tgcaagccac caagcttggc
     1501 gtcgaggtcg acaagcccgg tcgacaggcc cgagaggtgc ccttcgcgct ggtaggcagc
     1561 gttggccatc tcgatggcct gcgtcagagc ctggccgaat gtcaggaagc cttgcccgta
     1621 cttgcccttc tcggcgagct cgtaaagccg gttttccgcc tcctcgatct gctgctcggg
     1681 cggaaagtcg acggggctgt cgtacgccgt gttgaccagg tcctccccga tcgagatcag
     1741 cgcccggcgg atcgcgaggt cgtaaatcgt gcggccgtaa tccgccgcgt tgatgatcgt
     1801 cgtcgcgttg gcggccagcc ggcccaggta ttgcgggaca gtcagatcgt cgtcgatcgg
     1861 ctccgcgttc tcgaaaaatg tgcgcagcgt gatcggcgtg gcctgcttgc cggcctgaat
     1921 gagcttgcca gcgacctcgt aaatctgctg gtgcaacggg tcgtagaaat ggtgaggctc
     1981 aaggaagccg gcgacccggt ccatggcctc gttgttgatg agaatggcgc cgagcagggc
     2041 ctgctcggcc tcgatgttgt ggggcggctg acggaactcc agcggctcgc cggccgccgc
     2101 ctgagcgcgg agcttgtccg atgggaggag ggctgatttc gtcatgcgtg cgaagctatc
     2161 agatcccgcc caggatcagc tctgggaagt gtccccggga atgggccatc cccgcaatgc
     2221 acatcgacgg ccgaacgcgc ttgctacggt gcgaatcggc cagtgatttg aaacagacta
     2281 cgtgaattca gcgcgaaagt gcaaagttac tcgcccgcaa gacgtgcagg ctttgaaggg
     2341 gcggcgcctt ggctcaaccg ctgttcggcc tcgaacatgt aatcgcgggt gatcggcagc
     2401 gcgtcgatcc gcttcaccat ctggatctgg aacacgatga gcccgtggta gcggaacgcc
     2461 gcttcggacc cggcgaggta gaactcccac atccgg
//
LOCUS       DASUMG010000455         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_621389, whole genome shotgun sequence.
ACCESSION   DASUMG010000455 DASUMG010000000
VERSION     DASUMG010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621389"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1576)
                     /gene="argH"
                     /locus_tag="VFX46_07485"
     CDS             complement(173..1576)
                     /gene="argH"
                     /locus_tag="VFX46_07485"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEX5600461.1"
                     /translation="MDENSAGGPANKMWGGRFSMSPAQIMEEINASIEFDKVLAPQDI
                     RGSKAHAAMLAAQGIISAEDEAAIQAGLDQIMGEIESGQFTFSRSLEDVHMNIEARLK
                     DLIGAPAGRLHTARSRNDQVALDFRLYVRDRIDELDAALKELQLALARKAEAHAATIM
                     PGFTHLQPAQPVTFGHHLLAYVEMLARDRGRLQDARKRLNECPLGAAALAGTSFPIDR
                     EMTARALGFDRPTANSLDSVSDRDFALETLAAASIAAMHLSRLAEEIVIWMTPQFGFI
                     RLSDRFTTGSSIMPQKRNPDAAELTRAKVGRIAAAFQSLLMVMKGLPLTYSKDMQEDK
                     EVTFDALDNLALSIAAMTGMVEDMEPVPEVMRQAAGRGYSTATDLADWLVRRLNLPFR
                     EAHHITGRIVAAAESRGVELEELPLEAMQAIEPRITEDVYSVLGVENSVKSRTSYGGT
                     APQNVQEMARSWIKRLG"
     gene            1641..2297
                     /locus_tag="VFX46_07490"
     CDS             1641..2297
                     /locus_tag="VFX46_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX5600462.1"
                     /translation="MLSSTSARSGLIVLAAALVGFGAVYVTLGPDDNGRTSAPSTTPA
                     AETAAKLPETLPKGPGANPLSKGDMAAFVFKSEPEPLPEFSFVDATGVERSLKDWEGR
                     VVLLNLWATWCAPCRKEMPALDRLQQALGSDRFEVVALSVDRGGIEKSKAFFDEIGVK
                     ALKLYVDPSTRATSKLKAVGLPATLLIDEQGREIGRLTGPAEWDSEDAKRLIQAVLGS
                     "
     gene            complement(2400..3632)
                     /locus_tag="VFX46_07495"
     CDS             complement(2400..3632)
                     /locus_tag="VFX46_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600463.1"
                     /translation="MSLIDSLFVALSALRTNLLRSVLTTLGIIIGVGSVIIMVAVGSG
                     ATNQIDQQIAALGSNMLVVYPGDSRIRGRSAGAGTRLPLSERDLQAIRDKVPGVVAIS
                     GYLQGSAPVVNGNMNWTTQIGGVHDQYFEVRDWPLAEGREMTPQEVTSGARVAILGRT
                     VAEKLFGAGDPVGATIRIRNVPFQVIGVLSSKGQSSFGRDQDDIIFIPVTAARGRIIG
                     RSQVVNDQVGQIYVKFEPNADLAVAQEEIEMVLRERRRIRPGAEDDFNVRNLAEFMKT
                     RTAALSTMSWLLGATSAISLIVGGIGIMNIMLVSVTERTREIGLRMAVGGRRRDILLQ
                     FLVEAVTLSLLGGIIGIVIGIGGAAIVAVSAEWPILINAQAVGIALAAAAMTGILFGF
                     FPARRAALLNPIDALRSE"
     gene            complement(3629..>4053)
                     /locus_tag="VFX46_07500"
     CDS             complement(3629..>4053)
                     /locus_tag="VFX46_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600464.1"
                     /translation="LLPRTTALRQVMLPLLYAHPKPSNPEALARRRLEQVGLASRMDH
                     HPRQLSGGQQQRVAIARALVNNPKILLADEPTGALDTKTTAEIMQLFCELNEEGITVV
                     IVTHEPEVAEYARRVVVFRDGHIVEDRPQAGFQRRAAE"
BASE COUNT          789 a         1264 c         1251 g          749 t
ORIGIN      
        1 ataagtccag gttgggattt cctcgctaaa gcttgaggca agaagaatca ttaagctggc
       61 gcagagcacg ccagccagca acagaagccg catatccccg cgtcccccct aacctctggt
      121 ctaaagccct ctcccagagg atgcggaact caacttaaac tctttttcgt cattatccca
      181 gtcgcttgat ccacgaccgg gccatttcct gcacattctg tggcgccgtt ccaccatagc
      241 tggtccgact tttcactgag ttttcaacgc ctagcacgga gtagacgtcc tcagtgatgc
      301 gcggctcgat ggcctgcatt gcttcgagcg gcagctcttc cagctcgacg ccacgcgatt
      361 cggcggcagc aacgatacgc ccagtaatgt gatgggcctc gcggaacggc aaattcagcc
      421 ggcgcaccag ccagtccgcg agatccgtcg cagttgaata gccgcgccca gccgcctgcc
      481 gcatcacctc aggtacgggc tccatgtcct cgaccattcc ggtcatggcg gcgatagaga
      541 gcgcgagatt atcgagggcg tcgaacgtga cctccttgtc ctcctgcatg tccttggaat
      601 aggtgagcgg cagtcccttc attaccatca gcaggctctg gaaggccgca gcgatccggc
      661 cgaccttggc gcgcgtcagc tcggcagcgt ccggattgcg cttctgcggc atgatcgagg
      721 agccggtggt gaaccggtcc gagagccgga tgaaaccgaa ctgcggtgtc atccaaatca
      781 cgatctcctc cgcgagtcgc gacaggtgca tggccgcgat gctggcggcg gccagcgtct
      841 ccaatgcgaa atcgcggtcc gatacgctgt cgagcgagtt ggccgtcggc cggtcgaagc
      901 cgagcgcgcg cgccgtcatc tcgcgatcga tcggaaaaga tgttccggcc agcgccgcgg
      961 ctcccagtgg acattcattg agccgcttgc gcgcgtcttg gagccggccc cggtcgcgcg
     1021 caagcatctc cacgtaggcg agcagatgat ggccgaaggt gacgggctgc gcgggctgca
     1081 gatgcgtaaa gcccggcatg atcgtcgctg cgtgtgcctc cgccttgcgt gcgagagcaa
     1141 gctgcaattc cttcaatgcg gcatcgagtt catcgatgcg gtcgcgcaca tagagccgga
     1201 aatcgagcgc aacctggtca ttgcgcgagc gcgccgtatg gaggcgcccg gccggcgcgc
     1261 cgatgagatc cttgaggcgc gcctcgatgt tcatgtgcac gtcctcaagc gatcgagaga
     1321 acgtgaattg gccggactca atctcgccca tgatttgatc gagcccggcc tgaatggcgg
     1381 cctcgtcctc cgcagagatg attccctggg cggcgagcat tgccgcatgc gcctttgagc
     1441 cacggatatc ctggggggcg agcactttgt cgaactcaat ggaggcatta atctcctcca
     1501 tgatctgggc cggagacatg gagaagcggc ctccccacat tttattggcc ggaccgccgg
     1561 ctgagttttc gtccatggat cgggtctccc ggttcaggtc gcgacaccac acgttcgatg
     1621 aaagggccga gtctgcgaaa atgttgagct cgacatccgc cagatccggg ctaatcgtcc
     1681 tcgcggcagc actggtcgga tttggcgcgg tatacgtcac cctgggaccg gatgacaatg
     1741 gcaggacgtc tgcgccctcg accactcctg ccgccgaaac tgcggcgaaa ctaccggaga
     1801 cgctacccaa agggccaggc gcaaatccgc tgagcaaggg cgatatggcg gcgttcgtgt
     1861 tcaagagcga gccggagccg ctgcctgagt tctccttcgt cgacgccaca ggtgtggagc
     1921 ggagcctgaa ggattgggaa gggcgtgtcg ttctgttgaa tttgtgggcc acttggtgtg
     1981 cgccgtgccg aaaagaaatg ccagcccttg atcggctgca gcaggcgctc ggctccgatc
     2041 gcttcgaggt cgtggcgctg agcgttgatc gcggcggcat cgagaagtcg aaggccttct
     2101 tcgatgagat cggcgtcaag gcgctgaagc tgtacgtcga tccctcgact cgcgccacat
     2161 cgaaattgaa ggctgttgga ctgcctgcaa cgctcctgat cgacgaacaa ggccgcgaga
     2221 tcggtcgcct cacgggtccc gcggaatggg acagcgagga tgcgaaacgg ctcatccagg
     2281 cggtcctcgg aagctaaact agaactctcg ttctgcagca gatcaagggc gctgtttcaa
     2341 gaaagaacgg gccgctttgg cccgttcagt ttttcctcac gtaaacgacg gcgcctgttt
     2401 cactcgctcc gcaaagcatc gatcgggttg agcaacgcgg cgcggcgggc agggaagaag
     2461 ccgaacaaaa ttcccgtcat tgccgcggca gcgagagcaa tgcccacagc ctgggcattg
     2521 atgaggatgg gccactcggc cgagacagca acgatggcgg cgccgccaat gccgatcacg
     2581 atgccgatga tgccgccgag cagactgagc gtgaccgcct ccaccaagaa ctggagaaga
     2641 atatcacgcc ggcgtccgcc aacggccatg cggagaccga tctcgcgcgt ccgctccgtt
     2701 acagacacga gcatgatgtt catgatgccg atgccgccga cgatcagcga gatggcagac
     2761 gttgcgccca gcagccagct catggtggaa agcgccgcgg tcctggtctt catgaactcg
     2821 gcgaggttgc gcacattgaa atcatcctct gcgcccggcc ggatccgacg ccgctcgcgc
     2881 agcaccatct cgatctcctc ctgagcgacc gcaaggtccg cgttcggctc gaatttcacg
     2941 taaatctgcc cgacctgatc gttgacgacc tggctccggc cgataatgcg gccacgcgct
     3001 gccgtaacgg gaatgaagat gatgtcgtcc tggtcgcggc cgaagctcga ctgccctttg
     3061 gagctcagca cgccgatcac ctggaacggc acgttcctga tgcggatcgt tgcaccgacc
     3121 ggatcgccgg ccccaaagag cttttccgcc actgtccgac caaggatcgc cacgcgggca
     3181 ccggacgtca cctcctgcgg ggtcatttca cgaccttcgg cgagaggcca gtcgcgcacc
     3241 tcgaagtact ggtcgtgcac accgccgatc tgcgtggtcc agttcatgtt cccgttcaca
     3301 acgggagcac tgccctgcag atagcccgaa atagcgacca caccgggaac cttgtcgcgg
     3361 atcgcctgca gatcacgctc agaaagcggc aatctcgtac cagcgcccgc agaccgccct
     3421 ctgatgcgcg agtcgccggg ataaacgacc agcatgttgg agcccagagc agcgatctgc
     3481 tgatcgatct ggttggtcgc gccagagcct acggccacca tgatgatgac cgagccgacg
     3541 ccgatgatga tgccaagtgt cgtaagaacg gagcgcagca ggttcgtgcg taatgcggaa
     3601 agcgcaacga acagactgtc gatgaggctc attctgcggc cctcctctga aagcccgcct
     3661 gcggcctgtc ctcgacgatg tggccgtcgc ggaaaacgac cacacgacgc gcatattcgg
     3721 ccacttcggg ctcgtgcgtg acgatgacga ccgtgatgcc ctcttcattg agttcgcaga
     3781 agagctgcat gatctcagcc gtcgtcttgg tgtcgagcgc gccggttggc tcgtcggcca
     3841 gcaggatttt cgggttgttg acgagcgcgc gggcgatggc gacgcgttgc tgctgaccgc
     3901 ctgaaagctg gcgcgggtgg tggtccatgc ggctcgcgag ccccacctgc tccaaccgcc
     3961 gccgcgccaa agcttccgga ttcgacggct tgggatgggc atagagcagc ggcagcatga
     4021 cctgccggag cgcggtcgtg cgcggcagca gat
//
LOCUS       DASUMG010000456         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_782542, whole genome shotgun sequence.
ACCESSION   DASUMG010000456 DASUMG010000000
VERSION     DASUMG010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782542"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..848
                     /locus_tag="VFX46_07505"
     CDS             327..848
                     /locus_tag="VFX46_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5600465.1"
                     /translation="MSRTQDQSQLNSALHLLHRAGQRADELFTASMGEKDLTPRQYAV
                     LKAVAIMENPSQTALVEYTGIDRSTIADIVRRLIKRGMLQRRRTAHDARMYAVKLTPA
                     GREALESAEPVAKTTEEQLLSVLPAQQRAVVIQALSTIVAKLDADSNGSKGAEARAAS
                     AATTRRRRSSARQ"
     gene            1000..1449
                     /locus_tag="VFX46_07510"
     CDS             1000..1449
                     /locus_tag="VFX46_07510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEX5600466.1"
                     /translation="MTEVLFYHLERQPLERVLPTLLERTLERGWRAVVQCGSKERLEA
                     LDGWLWTYRDDSFLPHGTVRDGPADMQPIFLTTGNENPNGANVRFLVEGATISDFAPY
                     DRVVQIFDGHDAAAIELAREEWRRAKAAGCEVTYWQQADNGRWERRA"
     gene            complement(1450..1842)
                     /locus_tag="VFX46_07515"
     CDS             complement(1450..1842)
                     /locus_tag="VFX46_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600467.1"
                     /translation="MAEQRFTSKTFLLFSGLLIWAAHFMFVYSANAVACERVLQDVRV
                     LGIGLIPLTVMVATLAALAAAGSVLLTAIGWLGPLHGEPRDDPSSAFVRQITIVLSLI
                     SIVAITLSALPSLVVSPCEGLPQLVQGR"
     gene            complement(1848..>2150)
                     /locus_tag="VFX46_07520"
     CDS             complement(1848..>2150)
                     /locus_tag="VFX46_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX5600468.1"
                     /translation="LAPVLFIAAVGIQSWAEWQAGLRPTDHSYGAMVYLFAAVQGFYA
                     CVLLVMAVFTIARSLAGKLNSVRRVTFDNTMLLWHYAAVQGLVALVILSLFPRGLG"
BASE COUNT          464 a          633 c          675 g          378 t
ORIGIN      
        1 agagcatccc gcggcaaccc cgcctcctca agaaggctac ggcattcgag accccgtgcc
       61 tcggcgaggt cacgcaatgt cgcaaggatg gcggagcgaa cgagaacttc ggaagacatg
      121 acccccagat cattctttac ccgacgtaaa cgcccaactg cgccaatcgc cgcgattcct
      181 tgcatttagt gtcaagaagc atgtttcaca ggtcaagtct gcccacgtcg cttaacgtaa
      241 accctgtcga aaaatcagaa tcatgacaat gggttgagga cgtccagttc tgatcctgtt
      301 acatcttcct ggagcttttc cctacgatgt ctcggacgca agaccaatcc cagctgaact
      361 ctgccctgca cctccttcat cgcgccggtc agcgtgcaga tgagctgttc acggccagca
      421 tgggcgagaa ggatctcacg ccacggcagt acgcggtgct caaggcggtc gcaatcatgg
      481 agaacccaag ccagacggcg ctggtcgagt acacggggat tgatcgctcg acgattgcgg
      541 acattgtgcg gcggctgatc aagagaggca tgctgcaacg gcgccggacc gctcatgacg
      601 ccagaatgta tgccgtcaag ctgacaccgg ccggccggga agctctcgaa tccgccgagc
      661 ctgtggcaaa gacgacggag gagcagcttc tttctgtgct gccggcgcag cagcgcgcgg
      721 tcgttattca ggcactgagc accatcgtcg ccaagctcga tgcggactcc aatgggagca
      781 aaggcgcgga agctcgtgct gcatccgccg caacgacgag gcgcagacgt tcgtcagcgc
      841 ggcagtagca cattccggtt cggctgagct ccctgatctg cgatcggcgg gctcagtcgt
      901 cgactggctc ggttctccat cgatctgtcc gccgtggcct tgcttgcggt atgctgccat
      961 tggatggcag aacggcggtc gcgatgcgag ggagagcaag tgacggaggt gcttttctac
     1021 catctcgagc gccagccgct tgagcgggtc ctgccgactc tgctcgagcg cacgcttgag
     1081 cgcggctggc gtgcagtggt ccagtgtggg agcaaggagc gcctggaggc gcttgacggc
     1141 tggctctgga cctatcgcga cgacagcttt ctgccgcacg gtacggtccg cgacggccca
     1201 gcggacatgc agccgatttt tctgacgacg ggtaatgaga accccaacgg cgcgaatgtc
     1261 cgtttcctgg tggaaggggc gacgatctcg gacttcgcac cttatgatcg tgtcgttcag
     1321 atctttgacg gtcatgacgc ggctgccatc gagcttgccc gcgaagagtg gagacgagcg
     1381 aaggctgcag gctgcgaggt aacttactgg cagcaggccg acaacggacg ttgggaaagg
     1441 cgtgcctgat caccttccct gcacaagttg aggaagccct tcgcagggag acacgacgag
     1501 cgagggtagc gcgctcagcg tgatggcaac aattgatatc aatgagagca cgatcgtgat
     1561 ctggcgcacg aatgcgctgg agggatcatc gcgcggctct ccatggagcg gccccagcca
     1621 gccaatggca gtcagcaaca cggagcctgc agcggcaagc gccgcgagtg tggcgaccat
     1681 taccgtcagc ggaatcagcc cgatgccgag gacccggacg tcctgtaaaa cgcgctcgca
     1741 ggccacggca ttcgccgaat agacgaacat gaaatgggct gcccaaatga gcagtccact
     1801 aaaaagaagg aatgttttgg aggtgaaccg ctgttccgcc atagcggtca cccgagtcct
     1861 cgagggaaga ggctgaggat taccagtgcc acgaggccct ggaccgccgc gtagtgccaa
     1921 agcagcatgg tgttgtcgaa ggtgacgcgc cggaccgaat tgagcttgcc agccagcgag
     1981 cgcgcgatgg tgaaaacggc catgaccagg agtacgcagg catagaagcc ttgcacggcc
     2041 gcgaacagat agaccatggc tccgtagctg tgatccgtcg gccgcaaacc ggcctgccat
     2101 tcagcccagc tctgaatgcc aacggcggcg ataaagagca ccggggctag
//
LOCUS       DASUMG010000457         3041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_943786, whole genome shotgun sequence.
ACCESSION   DASUMG010000457 DASUMG010000000
VERSION     DASUMG010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943786"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1346)
                     /locus_tag="VFX46_07525"
     CDS             complement(216..1346)
                     /locus_tag="VFX46_07525"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX5600469.1"
                     /translation="MLHEFLTTNKSDLIARCRLKVAKRPAPRVAGKELAHGIPRFLDQ
                     LIKTLEVEQTSEPMRSRTVSGPAGGGAVVSEIAATAALHGRELSEQGFTVDQVVHDYG
                     DLCQAIADLAFERGVPIQVDEFRTLNRCLDNGIADAVTEYAFQRNSLVESNSVKALNE
                     RLGFLAHELRNLLHTATLAVMAIKAGNVGATGVTGALLDRSLIGMRNLIDRSLADVRI
                     TAGMPPRARLISLAEFVADVKISASLEAHARQCEFTVGDVDAELALDVDREMLFSAVG
                     NLLQNAFKFTQRHTEVSLNAYAAADRIRLDIADHCGGLPQQAADDMFRPFKQAGEDRS
                     GLGLGLAICRRSVEANSGVLSVRDVAGSGCVFTIELPRHVLA"
     gene            complement(1386..2594)
                     /locus_tag="VFX46_07530"
     CDS             complement(1386..2594)
                     /locus_tag="VFX46_07530"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX5600470.1"
                     /translation="MNLDWLYGLIHLGFWSYVWISLAMVQTTMMAVTVYLHRDATHRS
                     LDLHPALRHFFRFWIWFTSSMLTREWVAVHRKHHAFADLEGDPHSPVTAGLRRILLEG
                     SEFYKREARNPECVQQYGTGAPDDWIERRIYSRHRNAGIVAFVVLQLLLFGVPGIIII
                     AVQMLAMPVFAAGVINGLGHGIGYRNFEVENASRNIIPWGLFIGGEELHNNHHAFPSS
                     AKFSIRPWELDAGWLCVVVLRSLGLAKVKRVAPRPCVIAARQQADDHAAHAVIHTRMH
                     VLRAYAAQVVAPVLEHELGGELPRRAARAARKLLTRSPMLLDMNAKLRLQQLLAAHPM
                     LRTVHDYQEHLRQLWGTVASTKEDAAGRFASWCGRAEASGIRALEEFAGTLRRFALAD
                     RQNAGEGDVR"
BASE COUNT          626 a          959 c          901 g          555 t
ORIGIN      
        1 cggagatcaa cgtcgagacc ttcaaaggtg tcgttcaatt gagcggcttc gtgacctcga
       61 gcgcggacat cagcacagcg accaaagtgg cgaacgccgt cgccggtgta acttcggtca
      121 agaacgacat gcacgtgaag caatagctgc gagaagtctc caccgactct gcaaagttgc
      181 gagtagctgc ctcgcaggtg tcgaacctgc gaggtctacg ccagtacgtg tcgaggcagc
      241 tcgatcgtga aaacacagcc cgagccagcc acgtcgcgga cgctgagaac gccgctgttc
      301 gcctcgacgc tgcgccgaca gattgcgagg ccaagtccca ggccagatcg atcctcgccg
      361 gcctgcttga acggccgaaa catgtcgtcc gcagcttgtt gcggcaggcc gccgcaatgg
      421 tccgcgatgt ccaaacggat gcgatccgcg gcagcatagg cgttcaatga aacctcggtg
      481 tggcgctgcg tgaatttgaa agcgttctgc agcaagtttc ccaccgccga gaacagcatc
      541 tcccggtcga catcgagggc aagctcggcg tcaacatcgc cgactgtaaa ttcacactgc
      601 cgagcgtgag cctccagtga ggcggatatt ttcacgtcgg cgacgaattc ggcgagggag
      661 atcagtcgag cgcgtggcgg catccccgct gtgatgcgca cgtcggcgag cgatcgatcg
      721 atgagattgc gcatgccaat caaggaccgg tccagcagag ctccagtcac cccggttgcc
      781 ccgacattgc ctgctttgat cgccatgacg gccagcgtcg ccgtgtgcag cagatttcgt
      841 agctcgtgcg cgagaaatcc cagccgttca ttcagcgcct tgacgctatt gctctccacc
      901 agagaattgc gctgaaaagc gtattccgtg acggcatcgg cgatgccatt gtcgagacag
      961 cggttgagag ttcgaaattc atcgacctgg atcggcacac cgcgttcaaa tgccagatcc
     1021 gctatggctt ggcacagatc gccatagtcg tgaaccacct ggtcaaccgt gaatccctgt
     1081 tccgataact cgcgcccatg caacgccgca gttgcagcga tctcggagac caccgctccg
     1141 ccgcctgcgg gtccggacac cgtgcggctg cgcatcggct ccgacgtctg ctcgacttcc
     1201 agtgtcttga tgagctggtc aagaaaacgc ggaattccgt gagccagctc cttacccgcc
     1261 actctcggcg caggccgctt ggcgaccttg agccggcacc gggcgatcag gtcagatttg
     1321 ttcgtcgtaa ggaattcgtg gagcaccttg agagcatacg cttacgctcg ccgtcgctct
     1381 gtatgctagc gcacatctcc ttcacccgcg ttctgccgat cagcaagcgc gaagcgccgc
     1441 agcgtacccg caaactcctc cagtgcccgg atgccgctcg cttcagctcg cccgcaccag
     1501 ctcgcgaacc tgcctgccgc atcttccttg gtcgaggcga ctgttcccca gagttgccgc
     1561 aaatgttcct gataatcatg gaccgtcctg agcatcggat gagccgcgag aagctgctgc
     1621 aatcgaagct tggcattcat atccaacagc atcggactac gtgtgagtag cttccgggca
     1681 gcgcgggccg cacgccgggg cagctctcca ccaagctcat gctcgaggac cggtgcaacc
     1741 acctgcgccg cgtacgccct gagcacgtgc atgcgggtgt ggatgactgc atgcgccgca
     1801 tggtcatcgg cctgctgcct ggccgcaatg acgcaaggtc ggggagcgac acgtttgacc
     1861 ttggcgaggc cgagcgagcg cagcacaacc acgcacagcc aaccggcatc gagctcccac
     1921 ggtcgtatcg agaacttcgc ggacgacgga aatgcgtgat ggttgttgtg cagctcctct
     1981 ccgccgatga acagtcccca cgggatgata ttcctcgatg cgttctcgac ctcgaagttg
     2041 cggtagccga tgccgtggcc gaggccgttg atgacgccgg cggcaaacac cggcatcgcc
     2101 agcatctgga cggcaatgat gatgatgccc ggcacgccga acagcagcag ttgcaggaca
     2161 acgaaggcga caatccccgc gttgcggtgt cgcgaataga tgcggcgttc gatccaatcg
     2221 tcaggtgcgc ccgtgccgta ctgctgcacg cactccggat tgcgcgcctc acgcttatag
     2281 aattccgagc cttcgagcag aatccgtcgc aaccccgcag tcaccgggct gtggggatcg
     2341 ccttcgaggt ccgcgaaggc gtgatgcttg cgatgcaccg ccacccattc gcgcgtcagc
     2401 atgctcgagg tgaaccagat ccagaatcga aagaaatgcc gcagggcagg gtgcagatcg
     2461 agacttcgat gcgtggcatc ccgatgcagg tacacggtga ccgccatcat ggtggtctgg
     2521 accatggcca gtgagatcca cacgtagctc cagaagccaa ggtgaatcag tccgtataac
     2581 cagtcgaggt tcatgagcgc acgtcgggtg cgaagccaga tggagctgct tgcgggccta
     2641 tgcaggggca gttcaatgac gccgaggcat cgcgcgttgt tccccgaagg cttcaccgca
     2701 atcgacaggt catggtcatc atcgaggatg tcctgacctc agcgaggtag acccgtgcgt
     2761 cgaatcaaca tcggcaaggg atccgcccaa ccgaggcagc gattctggag cccctgtagt
     2821 ccgaacccat ggaccaattc gctcttcgtc ggcccgccca atcgccgccg atcgacggaa
     2881 cctcgcactc cgaggcaagg cggcaacaga gggcagccat cagtgcctgg gaagacgaag
     2941 gcggcagcgt tgccgcatcg cctgagagcc gccgcactta gccgcgcatt cgatgcaatc
     3001 ggtactcgtc atcgttcagc gtcatcaaca gcatgtgacc g
//
LOCUS       DASUMG010000458         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_783161, whole genome shotgun sequence.
ACCESSION   DASUMG010000458 DASUMG010000000
VERSION     DASUMG010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783161"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFX46_07535"
     CDS             <1..405
                     /locus_tag="VFX46_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600471.1"
                     /translation="ANSTFTFVPTNMVVGVIDNADATKHALRDLIADGFSEKEIALLT
                     DEEAVRRSGVIDEWSEVSVHIFRSTQKLPAFYDAPVIVRRLEQELHAGHHLIGVIAKD
                     GEMRERACGILKSHGGHFINFYGRWAAEALEP"
     gene            459..1031
                     /locus_tag="VFX46_07540"
     CDS             459..1031
                     /locus_tag="VFX46_07540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX5600472.1"
                     /translation="MSKPVRILGIAGSLRRDSYNRATLRAATELTPVRSSIETFELDG
                     LPGFNQDDEQNPPTKVVELKRRIREADAVLFVTPEYNYSVPGVLKNAIDWASRPYGDS
                     AWNGKPAAIMGASIGAIGTARAQYHLRQMMVFLNMFPVNQPEVMIGNASERFDADGNL
                     TDDATKEFIRQLLQNLVEWTRRISQASQRT"
     gene            1105..2961
                     /gene="nhaA"
                     /locus_tag="VFX46_07545"
     CDS             1105..2961
                     /gene="nhaA"
                     /locus_tag="VFX46_07545"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX5600473.1"
                     /translation="MRLDKLDPPVDSDHDHVLGPTDAELTLVEYGSYSCGPCHAVHEV
                     IEELRNRFGEQMRYVFRHLPMAGSEDAVRAAELAEYAAETTGGFWEVHEALMERGPVF
                     SAGDFERIAREGNLPTSAGHEAAVAAAQERVGEDIESARRSGARVTPTFFINGRRYAG
                     AWDESSLADAMLGALGHRMQSAAFQFVRWGPSSGLLLGLATVLALVLSNSPVGPAFQS
                     WWETRLGFQWGASTFDLTLLDWVNHGLLTIFFFVVGLEIKREFTVGHLASFRSGALPV
                     IAAFGGIVLPALIYALIAPPHLRHGWGIPIGTDTAFAVALIVLLGDRVPIELRVFLTA
                     AVIIDDIVAIIVIALFYSGEIGTIYLILSVAVVVLLMVLNRIGVYSALPYAVCGIVLW
                     FFLHEAGLHATLAGVILAVLIPTRPPANLRALLAQAATVIHEEDLHRDEAMRTGPSEP
                     ALRALDTIHARIESPADKLLRSVEPWSSYMVLPIFALANAGVAWSADVFQENAQLMGA
                     ITLGLVVGKSTGIVSASWLAVRSGLAVKPQAYSWRQLMGAGALGGIGFTMSLFIAGLA
                     FPNAADYAAAKIAIFIASVIAAVLGTIILYPRRDEESEPEVAEETDAVCSAI"
     gene            3013..>3654
                     /locus_tag="VFX46_07550"
     CDS             3013..>3654
                     /locus_tag="VFX46_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5600474.1"
                     /translation="MTEKLRDQTTSSIAEVLTGLRETAAWQERVYKEIHTHPELSFQE
                     TKTAALAATKLKEFGYEVLEGVGRTGVVGILRNGEGQTVLARADMDALPVRENTGLPY
                     ASTLTAVDDSGNTVNVAHACAHDAHVTCLLGAAVLLAKTRQSWGGTFIALFQPAEEVA
                     GGAKAMIDGGLRERIPKPDVAFAQHVLAYPAGKIGTQTGPVLSAGDSIRITLYG"
BASE COUNT          767 a          998 c         1131 g          758 t
ORIGIN      
        1 gcaaactcca cattcacctt cgtcccgacg aatatggtcg tcggggtcat tgataatgcc
       61 gacgcaacca aacacgcact acgcgattta attgcagatg ggttctcgga gaaggagata
      121 gctctcctga cggacgaaga agcggtgcgt cggagcggtg taatcgacga gtggagtgaa
      181 gtatcagttc atatctttcg ctcaacgcaa aaattgccag ctttctatga cgcgcccgta
      241 atcgtgaggc gactcgagca agagttgcac gccggtcatc acttgattgg agtgatcgcc
      301 aaggacgggg aaatgcgcga gcgagcatgc gggattttga aatcacatgg cggacatttc
      361 attaacttct atgggcgatg ggcggcggag gcgttggagc cgtaatccct gcacctgaca
      421 agtaaccaat tcactcgaac agtagggaga gcaaaaagat gagtaaacca gttcgcattc
      481 tcggcatcgc cggcagcctc cggcgcgact cctacaaccg cgcgacgctg cgagcagcaa
      541 ccgaactcac tcctgtacgc tcttcaatcg aaactttcga gcttgatggg ctccccggtt
      601 tcaatcagga tgatgagcag aacccgccta cgaaagttgt ggaattgaaa aggcgcatac
      661 gagaggcgga cgccgtgctt ttcgtgaccc cggaatacaa ctattccgtt cccggcgtgt
      721 tgaagaacgc gattgactgg gcatcgagac cttacggaga cagtgcgtgg aatggcaagc
      781 ccgcggcgat catgggcgct tcgattggag cgattggcac cgcgcgggcg cagtatcatt
      841 tgcggcagat gatggtcttc ctcaacatgt ttcccgtcaa ccagccggag gtaatgatcg
      901 gcaacgcctc cgagcgcttc gacgctgacg ggaatctgac ggatgacgcg acgaaagagt
      961 tcatccggca gttgctccag aaccttgtgg agtggacacg gcgaatttcg caagcgagtc
     1021 aacgaacgta aaaagaacgt cgcaattgat cggagcaagc gccggtgtca gaaaagtatg
     1081 cattgaagtg gagtcatggc ggatatgaga ctcgacaagc ttgatcctcc ggttgactcg
     1141 gaccacgacc acgttctggg gccgacagat gccgagctga cgcttgtcga gtacggcagc
     1201 tactcctgcg gcccttgtca cgccgttcac gaggtaattg aagagcttcg aaaccgattc
     1261 ggcgagcaga tgcgctacgt gtttcgtcat ctccccatgg ctggcagcga ggatgctgtt
     1321 cgcgcggcgg agcttgccga gtacgccgca gaaaccaccg gaggtttctg ggaagttcat
     1381 gaggcattga tggagcgagg gcccgtgttc tctgcgggtg acttcgaacg gatcgcacgc
     1441 gagggcaacc tcccaaccag tgcagggcac gaggccgcag tagcggcggc gcaggaacga
     1501 gtgggtgagg acatcgagag cgcacgacgg agtggtgcgc gggtgacgcc gaccttcttc
     1561 attaatggcc gccggtatgc gggcgcgtgg gacgaaagct cgttggcgga tgcgatgctc
     1621 ggcgcactgg ggcatcggat gcagtctgcc gcgttccagt ttgttcgctg gggcccatcg
     1681 tcgggtctac tcctggggct cgcgacggtg ctggcgctgg tgctcagcaa ctcgcctgtc
     1741 gggccggcgt tccagtcctg gtgggagacc agactaggat ttcaatgggg cgcatccaca
     1801 ttcgatctga cattgcttga ctgggtcaac cacggtctct tgacgatctt cttttttgtg
     1861 gtcgggcttg agatcaagcg tgagttcaca gtgggccacc tcgcgagctt tcgatcgggc
     1921 gcgctaccgg tgatcgccgc atttggtggc atagtcttgc cagcgttgat ctacgccctg
     1981 attgcgccgc cccacctgag gcacgggtgg gggattccaa tcggtaccga tactgccttc
     2041 gcagtcgctc tgatcgtgtt gcttggcgat cgtgtcccga ttgagctgcg cgtgttcctt
     2101 accgcggctg tcatcatcga cgatatcgtg gcaattatcg ttatcgcgct gttctacagc
     2161 ggcgagatcg gcacgattta tctgatcctg agcgtcgcgg tggtggtgct gttgatggtg
     2221 ctcaaccgga tcggcgtata cagtgcgctt ccatatgcgg tctgcggcat cgttctgtgg
     2281 ttcttcctgc acgaggctgg attgcatgcc acgttagcgg gtgtgatcct ggccgtgctc
     2341 atcccaacgc gtccgcccgc caacctaaga gcgctactcg cgcaagcagc gactgtgatc
     2401 catgaggaag acctccatag agacgaagcc atgcggactg gaccatctga gccggcgctg
     2461 cgggcactgg atacgatcca cgcccgcatc gagtcacccg ccgacaagct cttgcggtct
     2521 gtggagccgt ggtcgagcta catggtgctg ccgatcttcg cgttggcgaa cgctggcgtc
     2581 gcttggtcgg ctgacgtatt ccaggaaaac gcacaattga tgggtgcgat cacgttggga
     2641 ctggtggtgg ggaagtcgac cggcatcgtg tcagcctcgt ggctcgcagt acgcagcggc
     2701 cttgcggtaa agccccaagc ctactcttgg cgtcagttaa tgggcgccgg cgcgttgggg
     2761 ggaattgggt tcacgatgtc tttatttatc gccgggctcg cattccccaa cgcggccgat
     2821 tatgccgccg ccaaaatcgc aatcttcatc gcctcagtga tcgcggcggt gttgggaacg
     2881 atcattctgt acccgcgacg cgacgaagaa agcgaaccgg aggtagccga ggaaaccgac
     2941 gccgtctgct cggcaatttg agtcaacgag gcgtccacag cgcaagcgcc agtttgccaa
     3001 gaggtgaaat gaatgacaga gaaattacgt gaccaaacaa cgtcttcaat cgccgaggtt
     3061 ctgactggtc tgcgggaaac tgctgcatgg caggagcgtg tttacaagga aatacacacg
     3121 cacccagagc tgagttttca ggagactaag acggccgcgc tggctgcaac aaaacttaag
     3181 gagttcggct acgaagtcct cgaaggagtt ggtcgtacag gagtcgttgg cattctgcgc
     3241 aacggtgaag gacagacagt actcgcgaga gcggatatgg atgcgctccc ggtccgggaa
     3301 aacacaggcc tgccttacgc ttcgacgctc actgcagttg atgacagcgg caacacagtt
     3361 aacgtcgcac atgcctgcgc gcatgatgca catgtgacgt gtctgttggg cgcggccgta
     3421 ctcctcgcca agactcggca gtcgtggggt ggaaccttca tcgcgttatt ccaacctgcc
     3481 gaagaagtcg ccggcggcgc gaaagcgatg atcgacggcg gtctgcgtga gcgtatcccc
     3541 aaaccggatg tcgcgttcgc gcagcacgtg ctcgcttacc cagcaggaaa gatcggcaca
     3601 cagacaggac cagttctctc ggctggcgat agcattcgca tcaccctgta cggc
//
LOCUS       DASUMG010000459         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_141759, whole genome shotgun sequence.
ACCESSION   DASUMG010000459 DASUMG010000000
VERSION     DASUMG010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141759"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VFX46_07555"
     CDS             complement(<1..460)
                     /locus_tag="VFX46_07555"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX5600475.1"
                     /translation="MSTPNHATPLVALSGVVLDLETTGLDARSARVVQIGAVRIVNGR
                     IAEEAKLDRLVNPGVPIPATASAVHGITSGEVKEAPTFAEVASEIETFIGDTIVIGHA
                     VAYDIEVLKHEYALAKRPWRPVRSLCVRNLARLAAPTLADYSIEHLCQWLG"
     gene            652..2463
                     /locus_tag="VFX46_07560"
     CDS             652..2463
                     /locus_tag="VFX46_07560"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX5600476.1"
                     /translation="MFQSFEAPAELESSTDRINRLRSLLPELRVDAVLVPRADEHQGE
                     YVPASAERLRWLTGFSGSAGIAVITRRAGALFVDGRYTVQARSQVSKELEILQIPEAR
                     LSDWLREKLRPGAAVGFDPRLHTVSEIERLTEALADRKIKLKPLARNPVDRLWGAERP
                     PAPMGKVRPHPLKYAGRAAEDKIAEIQKTLKADGQDAVVLTLPDSIAWLFNIRGSDVQ
                     HNPVALAFAIVPAQGKPELFIAREKLDADAKAHLTFAKISQPEALAARLSQLKAAGKR
                     VRLDPNTASWWFAKQLGGPQRIARAQDPCILPKAKKNDVEIKGARAAHLRDGAAVVRF
                     LAWLDREAPSGRLDEIAAARKLEEFRAETNALLDISFDTISASGPNGAIVHYRVTEAT
                     NRRLKSGELYLVDSGGQYLDGTTDITRTIAIGRPSAEMRERYTLVLKGHIAIATARFP
                     KGTRGIDLDAFARRALWENGLDYDHGTGHGVGSYLSVHEGPQSISRRGMAVLEPGMII
                     SNEPGYYKEGAYGIRLENLVLVTKPEKVPGGDREMMSFETLTLAPFDRRLINVDLLTP
                     AEIAWIDDYHARVRQMLAPQLNEEDRSWLEAATAPLQ"
     gene            complement(2512..>2717)
                     /locus_tag="VFX46_07565"
     CDS             complement(2512..>2717)
                     /locus_tag="VFX46_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600477.1"
                     /translation="DRMPPRFGVLRPYLPFCRELECASWREPTGQRISFVAQYGKVAR
                     LIEQLTFSIMQRAAHFGQQRSAA"
BASE COUNT          530 a          862 c          843 g          482 t
ORIGIN      
        1 cgcccagcca ttggcacagg tgctcgatgc tgtaatcggc cagcgtggga gccgccagac
       61 gcgcgaggtt ccgcacacac aagctgcgca caggtctcca aggccgcttc gcgagggcat
      121 attcgtgctt gaggacctcg atgtcatagg ccaccgcatg gccgatgacg atcgtatcac
      181 caatgaaagt ttcgatctca gaggcaacct cggcgaatgt cggcgcttcc ttgacctccc
      241 cggacgtaat gccgtgaacg gcggaagctg ttgcgggaat gggcacgccc gggttcacca
      301 gccggtcgag cttcgcttcc tcggcgatcc ggccattgac gatgcgcacg gccccgattt
      361 gcaccaccct tgcggagcgc gcgtcgaggc ccgtcgtttc gagatcgaga acgacgcccg
      421 aaagcgcgac gagcggtgtc gcatgattcg gggtggacat tcggcctcgc ggtccggctg
      481 ttctccggta ctttgcgccc aagccgctcc acattgccag ctcaacacac ggttgtcagc
      541 tgagcgcatt gcgccagagg ctacgcctct gcttctatag cctgcaaatc cctaacaacg
      601 gcactggctt ctgcgcctgg tagctggcga caagaattgg gtggagattg catgtttcaa
      661 agcttcgaag cacctgccga gctcgagagc tcgaccgatc gcatcaatcg tctccgctct
      721 ctgcttccgg agcttcgcgt cgatgccgtg ctggtgccgc gcgccgacga gcatcagggg
      781 gaatacgttc cagcctcggc agagcgattg agatggctga cggggttctc cggatcggcc
      841 gggatagccg tgattacgcg gcgtgcaggc gcactgttcg tcgacggccg atatacggtt
      901 caggcccgct cacaggtttc gaaggagctg gaaatccttc agattcccga agcacgcctg
      961 tccgactggc tccgcgagaa gctgaggcct ggggcagccg tcggattcga tccgcgtctc
     1021 cacactgtga gcgagatcga gcggctcacc gaggcgttgg cagatcgcaa aatcaagctc
     1081 aagccgctgg cgcgaaaccc cgttgaccgg ctttggggcg ccgagcggcc tccggcaccc
     1141 atggggaagg ttcgcccgca ccccttgaag tacgccggcc gcgcggccga ggacaaaatt
     1201 gcggaaattc agaaaaccct gaaggcagac ggtcaggacg ccgtggtgct gaccctgccg
     1261 gactcgattg cctggctgtt caacatccgc ggcagcgacg tgcagcacaa ccccgttgcc
     1321 ctcgccttcg cgatcgtgcc tgcgcaggga aagccggagc tcttcattgc gcgggaaaag
     1381 ctcgatgccg acgccaaggc ccaccttacc tttgcgaaga tcagccagcc ggaggcgcta
     1441 gccgccaggc ttagccagct caaggcggcg gggaagcgtg ttcgcctcga tccaaatacc
     1501 gcctcgtggt ggtttgcgaa gcagctcggc gggccacagc gaattgcgag ggcacaggac
     1561 ccctgcattc tccccaaggc caaaaagaac gacgtcgaga tcaaaggtgc gcgggccgca
     1621 catcttcgcg acggcgcggc tgtcgttcgc tttcttgcct ggctcgaccg cgaggcaccg
     1681 agcggccgcc tcgatgagat cgctgccgcc cgcaagctcg aagaatttcg ggccgaaacc
     1741 aatgccctgc tggacatcag cttcgacacg atttcggcct ccggccctaa cggagcaatc
     1801 gtgcactacc gcgtgacgga agcaacgaac cgccggctga aatcggggga gctttatctc
     1861 gtcgactcag gaggccagta tctcgatggc acgacggaca tcacgcgcac aattgccatc
     1921 ggccgcccct ctgcggagat gcgggagcgg tacacgctcg tcctgaaagg ccatatcgcc
     1981 atcgcaaccg cgcgctttcc caaggggaca cgggggatcg acctcgacgc cttcgctcgc
     2041 cgcgcgctgt gggagaatgg gctcgattac gaccacggaa cggggcatgg cgtcgggagc
     2101 tatctttccg tgcacgaagg gcctcaatcg atctctcgac gcggaatggc cgtgctggag
     2161 ccgggcatga tcatctccaa cgagcccggc tactataagg agggcgccta tgggatccgc
     2221 ctggaaaacc tcgtgctggt caccaagccc gaaaaggtgc caggcggcga ccgcgagatg
     2281 atgagcttcg agacgttaac ccttgcgccg ttcgaccgtc gtttgatcaa cgtcgatctg
     2341 ctcacgccgg ctgagatcgc gtggatcgac gattaccacg cccgcgtccg acaaatgctg
     2401 gctccgcagc tcaacgagga ggatcggtca tggctggaag ccgcgaccgc tcccttgcag
     2461 tgattgcgaa agactttcgc tcgcaatgat ttccggccag cgctccgcgg actaagcggc
     2521 ggatcgctgc tggccgaaat gcgctgcacg ctgcatgatc gagaacgtga gctgctcgat
     2581 cagtcgtgca accttcccgt attgggcaac aaaagagata cgctggccgg ttggctcgcg
     2641 ccagctcgcg cattccagct ctctgcagaa tggcagatac ggccgtagga caccgaagcg
     2701 cggcggcatg cgatcga
//
LOCUS       DASUMG010000460         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_622394, whole genome shotgun sequence.
ACCESSION   DASUMG010000460 DASUMG010000000
VERSION     DASUMG010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622394"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VFX46_07570"
     CDS             <1..319
                     /locus_tag="VFX46_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX5600478.1"
                     /translation="RAENTSDRPVVGTATFNVTPEAAGAYFNKIECFCFQEQLLEPGE
                     AMDMPVSFFVDPAIVDDAIAGHLTQITLSYTFHPAEKTTAAAVSQSAGPAAERPSAGG
                     SGS"
     gene            427..1359
                     /locus_tag="VFX46_07575"
     CDS             427..1359
                     /locus_tag="VFX46_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX5600479.1"
                     /translation="MADSHAKHHDYHLVDPSPWPVLAALSAFIMAVGFIIWMHSMGGG
                     EGVFGIRGSSVFFIGAASLVAMAYFWWRDVVREAHSGHHTPVVQLHLRYGMILFITTE
                     VMFFAAWFWAYFDAALFPDGISHLPVILPDGTVEPSETVVGLVQRNELTGGQWPPVPS
                     DAFQSTFDPWGLPLVNTLILLLSGTTVTWAHHSLLENDRKGLIWGLALTVCLGVIFTL
                     LQAYEYGHAAFNYSGHIYGATFFMATGFHGAHVIIGTIFLFVCLMRALKGHFTPKQHF
                     GFEAAAWYWHFVDVVWLFLFACIYVWGAGTPAAH"
     gene            1438..1875
                     /locus_tag="VFX46_07580"
     CDS             1438..1875
                     /locus_tag="VFX46_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX5600480.1"
                     /translation="MANARVAWALRAAHIWHMADEHPIGPAVRAGLLGRCPRCGKGRL
                     FEGPLTLKVRQACEECGLSNAFVDSGDGPAVFVIIVLGFLMMGAALILEFKLSPPLWV
                     HLILWVPTALLLGFGLLRPMKATLIALQYRNRAEEARLEHGEH"
     gene            1879..>2680
                     /locus_tag="VFX46_07585"
     CDS             1879..>2680
                     /locus_tag="VFX46_07585"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX5600481.1"
                     /translation="MRQRPRGRGLVALTIATLAALAVLVSLGNWQWQRKAWKEELIAT
                     MDARASAEPLPPERWASLTCRSANEVGLAESCEFTTVRLVGRFDHTAERHLFTNAPQG
                     SSHEGPGYWVFTPFDLAGTNARIYVNRGFVPETNKDPQTRLEGQLSGEVEIIGQIRSA
                     EQRGTFTADSNPEANVWFLRDPRELLGASAAAPGELEHWRGPGPSGLEFYLDQISPTP
                     PGGLPTPRPSRIVLPNRHLEYALTWWGLAVTLMGVYAAFVIGRLRSREP"
BASE COUNT          444 a          818 c          867 g          551 t
ORIGIN      
        1 ccgcgctgag aacacctctg atcgccccgt ggtggggact gcgaccttca acgttacgcc
       61 ggaagcggcc ggtgcctatt tcaataaaat cgagtgcttc tgcttccagg aacagctgct
      121 cgagccgggc gaggcgatgg atatgccggt gagtttcttt gtcgatccgg cgatcgtcga
      181 cgatgctatc gccggtcatc tgacccagat tacgctgtcc tacaccttcc atcctgctga
      241 gaagacgaca gcggccgcag tgtcacagtc ggctggccct gcggcggaaa ggccaagtgc
      301 gggtggaagc gggtcgtgac atgaaaattg ctcaggtgag cagccgagga tttctgacta
      361 ccgggggcgc ggaccatctc gcgcgccatg cagggatggg atgcacgaag gacgacggag
      421 ctgaaaatgg ccgatagcca cgccaagcat cacgattacc atctggttga tccgagcccg
      481 tggccggtgt tggccgcgtt gtccgcgttc atcatggccg tcggtttcat catctggatg
      541 cactccatgg gcggcggtga aggggtgttc ggcatccggg gctcgagtgt tttcttcatc
      601 ggtgccgctt cgctggtggc aatggcctac ttctggtggc gggacgtcgt gcgggaggcc
      661 cacagcggtc accacacgcc ggtggtgcag ctccacctcc gctacggcat gatcctgttc
      721 atcaccacgg aggtgatgtt tttcgctgcc tggttctggg cgtatttcga tgcggcgctc
      781 ttccccgacg gtatctcaca tctaccggtt attctacctg acggaaccgt cgagccatcc
      841 gaaacggtag tcggcctcgt ccaacgcaac gagttgacgg gtggtcagtg gccgcctgtg
      901 ccctcagacg cattccagag caccttcgac ccgtggggcc tgccgctggt caacaccctc
      961 atccttctgt tgtcgggcac caccgtaacc tgggctcacc actccttgct ggagaacgat
     1021 cgtaaaggcc tgatttgggg cctcgcgctg acggtctgcc tgggcgtcat cttcacactg
     1081 ctgcaggcct acgagtatgg ccacgcggcc ttcaattact cgggccacat ctacggcgcg
     1141 acgttcttca tggccacagg cttccacggt gcgcacgtca tcatcggcac gatcttcctg
     1201 ttcgtgtgcc tgatgcgggc gctcaagggc cacttcacgc caaaacaaca ctttggcttc
     1261 gaggccgcgg cgtggtattg gcatttcgtt gacgtcgtgt ggctcttcct cttcgcctgc
     1321 atctacgtct ggggcgccgg aacaccggct gcgcactgat agcaccagtg ccgatgcagg
     1381 gtaggcgggg tagtcgataa ggctgccccg ccggtttctt ttgagagcac gggcgctgtg
     1441 gcgaatgccc gcgttgcgtg ggcgctcagg gcggcgcaca tctggcatat ggctgacgaa
     1501 catcccatcg gtcccgctgt gcgtgctggc cttctcgggc ggtgcccgcg ttgcggcaag
     1561 ggacgtcttt ttgaagggcc tctgacgctc aaagtgcgac aggcatgcga ggagtgcggc
     1621 ctcagcaatg ccttcgtcga cagtggcgat ggcccggcgg tgttcgtcat catcgttctg
     1681 ggcttcttga tgatgggcgc tgccttgatc cttgagttca agctatcgcc gcccctgtgg
     1741 gtgcacctga tcttgtgggt gccgaccgcg cttcttctgg gattcggcct gcttcggccg
     1801 atgaaggcga cgctgattgc gctgcagtac aggaaccgcg cggaggaggc ccgcctcgag
     1861 cacggcgagc actgagcaat gcggcaaagg ccgagaggca gaggtctcgt cgcgctgacc
     1921 atagccacgc tggccgcgct ggcggtgctg gtgagtctcg ggaattggca gtggcagcgg
     1981 aaggcctgga aggaggagct gatcgccacg atggacgccc gggcctcggc cgaaccgctg
     2041 ccgcccgagc gctgggcctc tctgacctgc cgatccgcaa acgaagtggg actggcagag
     2101 tcgtgcgaat tcaccacggt acgtcttgtc ggccgcttcg accacaccgc cgagcgacat
     2161 cttttcacga acgcgcccca gggctcctcg catgaaggcc ctgggtattg ggtcttcacg
     2221 cctttcgatc tcgccggaac gaatgccagg atttacgtga atcgcggttt cgtgccggaa
     2281 acaaacaagg acccgcagac ccggcttgag gggcagctct cgggcgaggt ggagatcatc
     2341 gggcaaattc gctcggccga acagcgcggc acgttcacgg ccgacagcaa tcctgaggcg
     2401 aacgtctggt tcctgcgtga ccctcgggag ctgcttggag cttccgccgc cgcgcccggc
     2461 gagctcgagc attggcgagg gccgggtccg agtgggctcg agttctacct cgatcagatt
     2521 tcgccgacac cgccgggcgg actgccgacc ccgcgaccaa gccgcatcgt gctgccaaac
     2581 cggcatctgg aatatgcact gacttggtgg ggtcttgcgg tgaccctgat gggggtctat
     2641 gcggctttcg tcatcggccg gctgcggtct cgggagccga
//
LOCUS       DASUMG010000461         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_461865, whole genome shotgun sequence.
ACCESSION   DASUMG010000461 DASUMG010000000
VERSION     DASUMG010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461865"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..867
                     /locus_tag="VFX46_07590"
     CDS             169..867
                     /locus_tag="VFX46_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX5600482.1"
                     /translation="MQYFLLGLAALLLVILALRGFTRADVKVMARQIRIVTGVVALAA
                     AAALTFRGLLSWATPLAMFGYWMLFGGSNAPVGGTAHKSPGQTSRVVTDYLEMELDHD
                     TGMMRGRVLKGSFAGREFESMSPAELAQLWQECRFADPQSAQIIEAYLDSMHPSWRED
                     MARAGADPGRAAASGKMTREEAYEILGLKPGASEEDIRRAHRELMMKLHPDRGGSAYL
                     AAKINLAKEVLLGR"
     gene            complement(895..>2025)
                     /locus_tag="VFX46_07595"
     CDS             complement(895..>2025)
                     /locus_tag="VFX46_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX5600483.1"
                     /translation="DPEQVTTARDMLTLALHLQDHFPQHYHFFSTRVFNFRGRSYRNH
                     NALLRTYAGTDGIKTGYIRMSGFNVVTSVRRGGRHVVGVVFGGKTAASRDAEMRALLD
                     RALGKASTKKTRQPMAVSFYRATPPKPAERPARVTQVAATSTPKPIKASETIPAEPQQ
                     PTTLAESPNAGSSTSSAPKITVAKVRPVSITPVGETQQKPLARSEPSSQPNPAVSRTD
                     SPGATGVVLGRPPSTLQEQAARLAQGQPAWHGLGFVGQANAMPAPPPTPAQRTASVAA
                     RDHQQLRPADSPPRPTPETGGYHVQIGAYSSLAEAEKALVATLEKVPTVLAEAAPRTI
                     PAVSGSRQIYRARFVGLDASAARNACEELRRRKIDCFVAKAE"
BASE COUNT          316 a          620 c          690 g          399 t
ORIGIN      
        1 cctactgccg tttcgacgcg ggctccgcgg cccagttgcg ggagctgctc tcggcggtgg
       61 ccgtctatgc agctggcggg cgggctgctc tcgagcggct tcggctggag ggctccgggc
      121 aaggggcccg gctgttgctc gagcagatga ggtgagaaag cagggggcat gcagtatttc
      181 ctgctcggct tagccgcact tctcttggtc atcttggcgc tgcgagggtt cacgcgggct
      241 gacgtcaagg tcatggcgcg tcagatccgc atcgtgaccg gtgtcgttgc tctggccgcc
      301 gcggccgctt tgaccttccg tggtctgctt tcctgggcca ccccgctggc gatgttcggc
      361 tactggatgc ttttcggtgg cagtaacgca ccagtcggcg gaacggcgca caagtcgccc
      421 ggccaaacat cgcgggtcgt caccgactac ctcgagatgg agcttgatca cgatacgggc
      481 atgatgcgcg gccgcgtgct caagggcagc ttcgcaggcc gggagtttga gagcatgagc
      541 ccggcggagc ttgcccagct ctggcaggag tgccgcttcg ccgatccgca gtccgcacag
      601 atcatcgagg cctatctcga tagtatgcac cccagttggc gtgaggacat ggcacgagcc
      661 ggtgccgatc ctggccgcgc cgcggcgtca ggcaagatga ctcgcgagga ggcttatgaa
      721 attctgggcc tcaagccggg tgcgagcgag gaggacatcc gacgtgccca tcgcgagctg
      781 atgatgaaac tgcacccgga tcgcggtggc tccgcctatc tcgccgccaa gatcaatctc
      841 gccaaggagg tgctgctcgg gcgctagttt cgcctgagtt acctctcgat cctgctattc
      901 cgccttggcg acgaagcaat cgatcttccg gcggcgcagc tcctcgcaag cattgcgggc
      961 tgccgacgcg tcgaggccaa cgaaccgggc cctgtaaatc tgccgcgagc cgctcacggc
     1021 agggatggtc cgcggggcag cttcggcgag caccgtgggt accttctcga gcgtggcgac
     1081 caaggccttc tccgcttcgg ccagcgacga atatgccccg atttgaacat ggtaaccgcc
     1141 ggtttccggc gttggccttg gcggcgagtc agcggggcgc aattgctggt gatctcgtgc
     1201 cgccaccgaa gccgttctct gcgcgggggt aggcggcggt gccggcatgg cgtttgcctg
     1261 ccctacgaaa ccgagcccgt gccacgctgg ttggccctga gccagtctgg cggcttgttc
     1321 ctgaagagtg gagggaggcc gtcccaacac gaccccagtc gcgccaggtg aatccgttct
     1381 actgactgcg ggattcggct gcgagcttgg ttcgctgcga gcgagtggct tctgttgtgt
     1441 ttctcccact ggcgtgatcg agaccggacg caccttcgcg acagtgatct taggcgcaga
     1501 agaggtagaa gagcctgcgt tgggcgattc agcgagtgtg gttggttgct gcggctcagc
     1561 cggtatggtc tcggatgcct tgattggctt cggcgtcgac gtcgctgcaa cttgggttac
     1621 acgggcaggc cgctccgccg gtttcggagg cgtggcccga tagaaggaaa cggccatcgg
     1681 ctggcgggtc ttcttcgtgg aagccttgcc cagcgcgcga tcgagcagcg ctcgcatttc
     1741 cgcgtcgcgg ctcgcagccg tcttgccgcc gaagacgacg ccgacgacat ggcgcccgcc
     1801 ccgccgcacc gacgtgacga cattgaagcc cgacatgcgg atgtatccgg tcttgatgcc
     1861 gtccgtccct gcgtaggttc gcaacagggc attgtggttg cggtagctgc ggcccctgaa
     1921 attgaacacc cgcgtcgaga agaagtgata gtgttgcggg aaatggtcct gcaggtgaag
     1981 cgccagcgtc agcatatcgc gcgccgtcgt cacctgctcg gggtc
//
LOCUS       DASUMG010000462         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_944409, whole genome shotgun sequence.
ACCESSION   DASUMG010000462 DASUMG010000000
VERSION     DASUMG010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944409"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFX46_07600"
     CDS             <1..365
                     /locus_tag="VFX46_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600484.1"
                     /translation="MIKKSFVFTALVFATCLVPPPATAEEETGLAAIHDQRREGNRIC
                     MSSHFHYGSSSGHKTRKEAEIAAIRDWAGFTAWEYGLPWGSYRLAASKSMNCSQSGAT
                     WGCQVEARPCKPITRKKG"
     gene            complement(404..1606)
                     /locus_tag="VFX46_07605"
     CDS             complement(404..1606)
                     /locus_tag="VFX46_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX5600485.1"
                     /translation="MALLQLIIWLIALAILPAALAGPAAAQACTKQDIASLIDSTAAK
                     LRQLNAAHQPELQAKLRQLGDRLGWSEEEREAKAAEFLDDDETRALDEQASKLLVELD
                     VIGDEAEIDPTTCQSRLEKLKKISAQLIEITTAKAAHASARIDAELNALGLSRRSADQ
                     PGGKAAPRTATRTEPPRSQPPMSDAPAPGPPEARDARPAPPAVSGWDTDTFRHGTPDE
                     ADRAPPPDVLADLSSDLHRSEELEFSVEEIRAAGRGFFGSLSAGLASVIEYAFQKYGR
                     PNGYILGSEGGAALLAGLRYGDGRLVTKFAGEQKVYWQGPSVGYDVGLAGSRVMFLVY
                     NLERPEDLYARFMGVDGSAYVVGGVGVTFLKKGRIVLAPIRTGLGVRVGANVGYLKFT
                     PERSLNPF"
     gene            1747..2112
                     /locus_tag="VFX46_07610"
     CDS             1747..2112
                     /locus_tag="VFX46_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600486.1"
                     /translation="MMMTRYLLAAIAMIILTGPLASAAQALGLDFLHAQARVGNKVCM
                     TEHEHYGEGTMPTRRAAQSAAIRAWSDFTSFEYGRIWGSYRLAEGKRMECSQTGSGWL
                     CKTYARPCRSAKYGPPRRR"
BASE COUNT          454 a          694 c          691 g          431 t
ORIGIN      
        1 tgatgatcaa gaaatccttt gtgtttactg ctcttgtttt cgcgacctgc ctcgtgccgc
       61 cgccagccac agcggaagag gaaacgggat tggcggccat ccacgatcag cgccgtgaag
      121 gcaaccgcat ctgcatgtcg agccactttc attacgggtc gagctcaggc cacaagactc
      181 ggaaagaggc cgaaatcgcg gcaattcgcg actgggctgg cttcacagcg tgggagtacg
      241 gacttccctg gggcagctac cggctagcag ccagcaagtc gatgaactgc tcgcagtcag
      301 gtgccacctg gggctgccag gtggaggccc ggccgtgcaa gccgatcacg cgtaagaaag
      361 gatagttccg cacttgttct atcagctcag cgtcagtccg acgtcagaag ggattgaggc
      421 tgcgctctgg tgtgaatttc agatagccga cattggcgcc gacccgcacg ccgagtcctg
      481 tgcggatcgg cgccagcacg atgcggccct tcttcaggaa ggtcacgccg acgccaccga
      541 cgacgtaggc cgacccatcc acgcccatga accgggcgta gagatcctcg gggcgttcca
      601 gattgtagac gaggaacatg accctggagc ccgccaggcc gacatcgtaa ccgaccgacg
      661 ggccctgcca gtagaccttc tgctcgccag cgaacttcgt aaccagccgt ccgtcgccgt
      721 accgaaggcc ggcaagcaac gcggctccgc cttcggaccc gaggatgtat ccgttggggc
      781 gcccatattt ctggaaggcg tactcaatga cgctggcaag accggccgaa agcgagccga
      841 aaaagccgcg gcctgcagcc cggatttctt caacagaaaa ctccagctcc tccgatcgat
      901 gaaggtcgga agaaaggtcc gccaggacgt cgggaggagg agcacgatct gcttcatccg
      961 gcgtcccgtg acggaacgtg tcggtatccc aaccgctgac cgccggcggc gccggacgag
     1021 cgtccctggc ctccggtggg ccaggtgccg gtgcatcgct catcggcggt tgagatctcg
     1081 gcggctccgt tcgggtagcc gtcctcggtg ctgctttgcc accaggctgg tccgcggagc
     1141 ggcgagatag gcctagcgcg ttcaactccg catcgatgcg cgctgaggca tgggcagcct
     1201 tggccgtcgt aatctcgatg agctgggcgg aaatcttttt cagcttttcc agccgcgact
     1261 ggcatgtagt cggatcgatt tccgcctcgt cgccaatgac atcaagctcg accagtagct
     1321 tgctcgcctg ctcatcgagc gcacgggtct cgtcatcatc cagaaactcg gctgccttgg
     1381 cttcacgttc ctcctcgctc cacccgagcc gatcgccaag ctgcctcagt ttggcctgca
     1441 gctccggctg gtgagcggca ttgagttgtc tcagctttgc cgcggtggag tcgatgagac
     1501 tggcgatgtc ctgcttcgtg caagcctgag cggcagccgg cccggccaat gcagccggca
     1561 agatcgccag cgcaatcaac catatgatca gctgcaataa agccatgaaa ataatcgccc
     1621 aagtttgctg atgcgttcgg cagcgtttgt ccaggtaaga caaattttgc catagacaac
     1681 ggtcgtccgt gcgacaaccg caggtggcgg tatcatctgc ctgaatatca gaatgaaaat
     1741 ggaggcatga tgatgacccg atacctgctt gcggctattg ctatgataat actcactggg
     1801 ccgcttgcat cagctgcaca ggcgttgggg ctggatttcc ttcatgcaca agcgcgggtt
     1861 ggcaataagg tctgcatgac cgagcacgag cactacggtg aaggcacgat gccgaccaga
     1921 cgtgccgcgc agagtgccgc catccgcgcc tggagcgact ttacctcctt cgaatacggc
     1981 agaatttggg gcagctaccg actggccgag ggcaagcgga tggagtgctc tcagacggga
     2041 agcggctggc tttgcaagac gtacgcgcgc ccgtgccgct cggcgaaata cggcccaccc
     2101 cggcggcggt gatatcagcc ggtccaagca ctccgcttct gtcattacag atgaagactg
     2161 ggcgcctctc cccttggcgt cctggtggtc tgctgagccc attcgattcc ggggagcaca
     2221 gagcatcgaa atgtggtaag cacatcgaac gtgccccgga gcacggcggt
//
LOCUS       DASUMG010000463         6312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_461937, whole genome shotgun sequence.
ACCESSION   DASUMG010000463 DASUMG010000000
VERSION     DASUMG010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461937"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1196)
                     /gene="murG"
                     /locus_tag="VFX46_07615"
     CDS             complement(78..1196)
                     /gene="murG"
                     /locus_tag="VFX46_07615"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX5600487.1"
                     /translation="MLAAGGTGGHLFPALALAQELGRRGYAVDLMTDMRGDRYGSDFP
                     ARDIYLVPSATIGERRSALETIRTAITLARGILTAYRQLASVKPGAVIGFGGYPSFPP
                     VMAAWLRRIPTAIHEQNSVLGRANRLLAKRVTAIATSFPSVRYLDASLERKVRLTGNP
                     VRDAVIDWSTRAYVPPEPAGEFRLLVFGGSQGARFFSDLLPQAVALLKPETRSRLRIV
                     QQCREEDLGRVRQAYAALEVDMELATFFRDLPEKMAAAHLVIARAGASSVAELTVMGR
                     PSILVPLPHAVDNDQLRNATQLARNGGASCIEQKDLTPERLASEIERLAANPDELARM
                     AAAAKSQGRADAVIRLADLVEELMGARQPRPPIEQDRN"
     gene            complement(1211..2389)
                     /gene="ftsW"
                     /locus_tag="VFX46_07620"
     CDS             complement(1211..2389)
                     /gene="ftsW"
                     /locus_tag="VFX46_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX5600488.1"
                     /translation="MISRADRSIVTEWWFTVDRVLLAAILLLAGSGLVLSLAASPAVA
                     LKKGLHAFHFVERHAAFSVIGVGIMLAISMMQPRMIRRVSLIVFLAALLLMICVLNFG
                     DEINGARRWLRLGSFSIQPSEFAKPAFIVLSAWAFAETQKQIDMPARSIAVGLLLVLA
                     ALLLMQPDVGQTFLVGSIWATLFVLSGQPLFWVGIFAAIGIFGLASAYLTLDYVRLRV
                     DRFLASGGDEYSQTARALQSFVEGGFFGRGPGEGTIKTALPDAHTDFIFAVIAEEYGA
                     VACLALVALFAIIVLRAYFRTFTEPDPFARLAVSGLSLLFGLQAAINMGVNVGLLPAK
                     GMTLPFISAGGSSLVSTFFTMGIILALTRRKPGALRLRTPHSALKNENRLGGLGPATQ
                     "
     gene            complement(2392..3801)
                     /gene="murD"
                     /locus_tag="VFX46_07625"
     CDS             complement(2392..3801)
                     /gene="murD"
                     /locus_tag="VFX46_07625"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX5600489.1"
                     /translation="MIRVSIFEGRKVALFGLGASGRATALALQAGGAEVAAWDDSAAA
                     CAAARDEGISVVDLSTADWGEFAALILAPGVPLTHPEPHWTVVKARTAGIEVIGDIEL
                     FCRIRTQLCPDAPLAAITGTNGKSTTTALLAHILQSAGRDVALGGNIGTPILALSEPA
                     PDRVHVVELSSFQIDLTPSLAPTVGVLLNLTPDHLDRHGTMENYAAIKERLVAKSRHA
                     IVSTDDAYCRAIAQRLQERADGSVDVFSVLGPVPGGWSANGTRLVCDAAWPGFHGPFA
                     DLDGIGTLRGRHNAQNALAACVAALRLGVRPEQLSPALATFPGLPHRMEEVGRLGRVL
                     FINDSKATNADSTEKALSAFPRDIFWIVGGRPKEGGIEPLRDYFPRIAKAYLIGESAE
                     AFAATLGNSVPFEHSGTLEVALQAAARDAAQCAGAEPVVLLSPACASYDQFKNFEHRG
                     DTFRQLVAALPGVRVTNRP"
     gene            complement(3842..4924)
                     /gene="mraY"
                     /locus_tag="VFX46_07630"
     CDS             complement(3842..4924)
                     /gene="mraY"
                     /locus_tag="VFX46_07630"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX5600490.1"
                     /translation="MLYELVNFSDYINALNVFRYITFRTGGAIMTALLFVFLFGPVII
                     DALRVKQGRGQPIRDDGPQTHLAKRGTPTMGGLMILSGFVVATLLWANLANPYVWVVL
                     GVTLAYGAIGFCDDYMKVTKSTVAGFSGRIRFSLELGIAAIATLILMYLSMPEMATAL
                     TVPFFKDIVIDLGPFFVLLGVVVIAGAGNAVNLTDGLDGLAIVPVMIAAATFGLIAYL
                     AGNVNFARYLQIHYVPGTGELAIVCGALIGAGLGFLWFNAPPAMIFMGDTGSLALGGA
                     LGTIAVATKHEFVLAIVGGLFVIETLSVIIQVVSFKLTGKRVFRMAPLHHHFEQKGWK
                     ESTVVVRFWIISVVLALIGLSTLKLR"
     gene            complement(4997..>6312)
                     /locus_tag="VFX46_07635"
     CDS             complement(4997..>6312)
                     /locus_tag="VFX46_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HEX5600491.1"
                     /translation="VSIDSRTLQKNDLFIALKDVRDGHEFVPAAFAAGASAAVVSRSY
                     RRQPGDGALLTVDETLSALQAIARAARARTAARVVAVTGSVGKTGTKEALRACLSRVG
                     ATHAAEKSYNNHWGVPLTLARMPAQSQFCVVEMGMNHRGEIAPLALLARPHVAIITTV
                     EPVHIGYLGSLEAIAEEKSDIFLGLERNGVAIIKRDSPQFPIMQSAAERQGARVISFG
                     RSQDANIRATDIKLHPNGSDVTVDALGQTLSYRVGAPGAHLVENSLAVVAALLALEVD
                     FARALPALAEISPPAGRGQRMVLEVGGGKALLIDESYNANPASMRAALEALATVPRID
                     HPRRVVVMGDMLELGEQAAQLHVGLKEAIDAAGVDLVFACGPNMAHLFAELAPSQRGL
                     WAETSDGLTDRLLDSIRPGDVVMIKGSLGSRMAPLVEALKSRFGR"
BASE COUNT         1256 a         1974 c         2008 g         1074 t
ORIGIN      
        1 ccgctcatgc cgatgccgcc gatgccaatg atgtgaaagg gcccgatccc gcggggtatt
       61 tccatcgaat ttcttcctca gttcctgtct tgctctatgg gcggacgtgg ctgccgcgcg
      121 cccatgagct cctccacgag atcggcgagg cggatcactg catcggcgcg tccctgtgac
      181 ttagctgcgg ccgccatgcg tgccagctca tctggattcg cggccagccg ctcgatttcg
      241 cttgcaagcc gttccggcgt cagatccttc tgctcgatgc atgaagctcc cccgtttcga
      301 gcaagttgcg ttgcgttccg caattggtcg ttatctacgg catgtggcag cggaaccaag
      361 atcgagggcc gccccataac cgtcagctcg gccacggacg acgccccggc acgtgcgatg
      421 accagatgcg ccgcggccat cttttccgga agatcgcgaa aaaaggttgc gagttccata
      481 tcgacctcga gggccgcata cgcctggcgc acacggccga ggtcttcttc gcggcattgc
      541 tggacgattc tgaggcggct gcgggtctcg ggcttcagta gggcgaccgc ctggggcaac
      601 agatccgaga agaagcgcgc accttggctc ccgccaaaaa cgagcaacct gaactcgccg
      661 gctggctcgg gtggcacgta ggcccgcgtc gaccagtcga tcaccgcatc gcggaccgga
      721 ttgccggtga gccgcacctt acgttccagg ctcgcatcca gatagcgcac tgacgggaag
      781 gatgtggcga tcgcagtcac ccgtttggcg agcaggcggt tcgcacgtcc gagcaccgag
      841 ttctgctcgt gaatggccgt ggggatgcgc cggagccagg cggccatcac tggagggaag
      901 cttggatagc cgccgaaccc gatgactgct ccgggtttaa cggaggcaag ctgccggtag
      961 gccgtgagaa ttccgcgggc aagggtgatg gcagtccgga tggtttcgag cgcgctgcgc
     1021 cgctcgccga tcgtggcaga agggacgaga tagatgtctc gcgccggaaa atcgctgccg
     1081 tagcgatctc cccgcatgtc ggtcatcaaa tcgaccgcgt agccacgccg gccaagctct
     1141 tgcgccagcg ccagcgccgg gaagagatgg ccgccagtgc caccggccgc aagcatcacc
     1201 gagggagcat tcactgcgtt gccggtccca gtccgcctag tctgttttca ttcttgagcg
     1261 ccgaatgcgg cgttctcaag cgcagggcgc caggcttgcg tcgggtcagc gcgaggatga
     1321 tgcccatggt aaagaacgtc gatacgagcg acgagccgcc agccgagatg aagggaagcg
     1381 tcatgccttt tgccggcagc aggcccacgt tgacacccat gttgattgcg gcctggagcc
     1441 cgaacagcaa cgatagcccg ctcacggcaa ggcgcgcgaa tgggtcgggt tccgtaaacg
     1501 ttcgaaaata ggcgcgcaag acgatgatcg cgaacagcgc gacaagcgcc agacaggcca
     1561 cggcgccgta ctcctccgca atcaccgcga agatgaagtc cgtgtgagca tccggcaggg
     1621 ccgtcttgat cgtgccctct cccggcccgc ggccaaagaa gccaccctct acgaaggatt
     1681 gcagagcacg cgccgtttgg gagtattcgt cgccgcccga agccaggaag cggtcaacgc
     1741 gcaagcgcac gtaatccagg gtcaaatagg cgctagccag tccgaagatg ccgatggcgg
     1801 cgaatatacc gacccagaat aggggttggc ctgatagcac gaacagcgtc gcccagatcg
     1861 agccgacaag gaatgtttgg ccgacgtcgg gctgcatgag cagaagcgcc gccagcacca
     1921 acaacagccc gaccgcgatg gaccgcgcgg gcatatcgat ctgcttctgc gtttccgcga
     1981 acgcccaggc actgagcacg atgaaggcgg gcttcgcaaa ttccgatggc tgaatggaaa
     2041 agctgcccaa tcgtagccaa cggcgggcgc cgttgatctc atcaccgaaa ttgagcacgc
     2101 agatcatcag caggagcgcg gcgagaaaga cgatgagcga aactcgccgg atcatccgcg
     2161 gctgcatcat cgagatggcg agcatgatgc cgaccccgat gaccgagaag gccgcatgcc
     2221 gctcgacgaa gtggaacgcg tgcagacctt tcttcaaggc aaccgccggg cttgccgcca
     2281 aggacagcac cagaccgctg cccgcgagaa gcaggatcgc agcgagcagc acccgatcga
     2341 ccgtgaacca ccattcggtg acgatactgc gatctgcccg cgagatgatc ttcatggccg
     2401 attcgtcacc ctcacaccgg gcagcgccgc gaccagctgg cggaatgtgt ccccgcggtg
     2461 ctcgaaattt ttgaactggt cgtaggaggc acaagccggc gagagcagaa cgaccggctc
     2521 agcgcctgcg cactgggctg catctcgcgc ggccgcctga agggcaacct cgagcgtccc
     2581 cgaatgttcg aacggcacgc tatttccgag ggtggccgcg aacgcctcgg cagattctcc
     2641 gatcagatag gccttcgcga tgcgcgggaa gtaatctctc agtggctcga tgccaccctc
     2701 tttcggccgg cctccaacga tccagaaaat gtcgcgcgga aaggccgaaa gggccttctc
     2761 cgtggagtcg gcgttggtgg ctttcgaatc gttgatgaag agaacgcgcc ccaaccggcc
     2821 cacctcttcc atgcggtgcg gcaagccggg aaaggtggcc agcgccggcg aaagctgttc
     2881 gggcctcaca ccgaggcgca gtgcagccac gcaagccgcg agcgcattct gggcgttgtg
     2941 gcgaccgcgt agtgttccga tgccgtcgag atcggcgaat ggcccgtgaa agccgggcca
     3001 ggctgcatcg caaacgaggc gcgtgccatt ggccgaccag cctccgggaa ccgggccgag
     3061 cactgagaaa acgtccacgc tgccgtcggc acgctcctgc agtcgctggg caatggcccg
     3121 gcaataggca tcgtccgtgc tgacgatcgc gtgccgggat ttcgcgacca gccgctcctt
     3181 gatggccgcg taattctcca ttgtcccgtg gcgatcgaga tgatcaggcg tgaggttcag
     3241 caggacccca acggtcggcg caagcgatgg cgtgaggtcg atctgaaacg acgatagctc
     3301 cacaacgtgc acgcgatcgg gcgccggttc ggagagcgcc aggatcggcg tcccgatgtt
     3361 gccgcccagg gcaacgtcgc ggcctgccga ttgcagaatg tgggccaaca gcgcggttgt
     3421 cgtcgatttg ccgttcgttc cggtgatcgc ggcgaggggt gcgtcgggac agagctgcgt
     3481 gcgtatccgg cagaacagct caatatcgcc gatgacttcg atgcccgccg tacgggcctt
     3541 gaccactgtc cagtgcggct cagggtgagt caggggaaca ccgggggcaa gaataagcgc
     3601 ggcgaactcg ccccagtcgg ccgtactcag atcgacgacg gagatccctt cgtctcgtgc
     3661 cgcagcgcaa gctgccgcac tgtcgtccca ggcggcgact tcggcgcctc cggcctgcaa
     3721 agcaagcgcc gtcgcgcgcc ccgaggcccc gagaccgaac agggcgacct tccttccctc
     3781 aaagatgctg acgcgaatca tcgcccgatt ttagtggatt tcgcgccgcg gtgttcagac
     3841 gctaacgcag cttcagcgtc gagaggccga tcagcgcaag caccacggaa atgatccaga
     3901 accggacaac cacggtcgat tccttccagc ccttctgctc gaaatgatgg tgcagcgggg
     3961 ccatgcggaa cacgcgtttg cccgtcagtt tgaacgagac cacctggatg atgacggaca
     4021 gggtctcgat cacgaacagc ccgccgacga tcgcgaggac gaactcgtgc ttcgtggcca
     4081 ctgcgatggt tccaagggcg ccgccaagcg ccagcgagcc tgtgtcgccc atgaagatca
     4141 tcgcaggcgg agcgttgaac cagagaaagc cgagacctgc tccgataagc gcgccgcaaa
     4201 cgattgccag ctcgccggtt cccggcacgt aatggatctg gagatagcgc gcgaaattca
     4261 cgttgcctgc aaggtaggcg atcagcccga acgtcgccgc ggcgatcatt accgggacga
     4321 tggcgagacc gtccaatccg tcggtcaagt tgacggcatt gcctgctcca gcgatcacta
     4381 caactccaag gagcacgaaa aacggcccga gatcgatgac gatgtccttg aagaagggaa
     4441 cggtgagcgc cgtcgccatc tccggcatcg acaggtacat gagaatgagc gtcgcgatgg
     4501 cggcaatgcc cagctcaagc gagaacctga tgcgtcccga gaagccggcc accgtgcttt
     4561 tggtcacctt catgtagtcg tcgcagaagc cgatcgcgcc gtaggcgagc gtcacgccca
     4621 agaccaccca gacgtacgga ttggccagat tggcccacag cagcgtggcc acgacgaagc
     4681 cggacaggat catgagcccg cccatggtcg gtgtaccccg tttcgcgagg tgagtttgcg
     4741 ggccgtcgtc gcggattggc tgcccgcggc cttgctttac gcgcaatgca tcaatgatga
     4801 cggggccgaa gagaaagacg aacagcagcg ccgtcatgat ggcgccgccg gttcggaagg
     4861 ttatgtagcg gaagacattg agggcattga tgtagtcact gaaattaaca agctcgtaga
     4921 gcatgaagca gtccttcgaa actccgtggc ctcagcctca tcgcataggc cgtcttccgc
     4981 attgtaagcc gggcagctaa cggccgaacc ggctttttaa ggcctctacc agcggtgcca
     5041 tgcggctgcc gagagagccc ttgatcatga cgacatcacc cgggcggata gaatcaagca
     5101 ggcggtcggt aaggccatcc gatgtttcag cccagagccc tcgttggctg ggcgccagtt
     5161 ccgcaaacag gtgcgccatg ttcggaccgc atgcaaagac cagatcgacg ccggcggcgt
     5221 caatcgcttc tttgagcccg acgtgcaact gcgcagcctg ttccccgagt tcgagcatgt
     5281 cgcccataac aaccacacgt cgagggtggt caatccgagg tacggttgcg agtgcttcga
     5341 gcgcggcgcg catcgaggcg gggttggcat tatagctctc gtcgataagc aaagccttgc
     5401 caccgccaac ctcgagcacc atccgttggc ctcgtcccgc gggtggtgaa atttcggcaa
     5461 gagcgggtaa ggcccgtgcg aaatcgactt cgagagccag cagggcagcc acgaccgcca
     5521 acgagttctc gacgagatgg gcgccgggag ccccaacccg gtagcttagg gtctgtccga
     5581 gcgcatcgac cgtcacgtct gacccattcg gatgaagctt gatgtctgtc gcccggatgt
     5641 tggcgtcctg gctcctcccg aagctgatca cgcgagcgcc ttgccgctcg gcggccgact
     5701 gcatgatcgg gaattgcggg ctgtcgcgct tgatgattgc caccccgttc cgctccaggc
     5761 cgaggaagat gtctgatttt tcctccgcga tggcttcgag ggagccgaga tagccgatgt
     5821 gcaccggctc gaccgtcgtg atgatcgcca cgtgcgggcg ggcgagcagc gccagtggag
     5881 caatctctcc gcggtggttc atgcccatct cgacgacgca gaactgcgat tgggctggca
     5941 tgcgggcgag ggtaaggggc acgccccagt gattgttgta ggatttttca gccgcgtgcg
     6001 tcgccccaac gcgcgacagg caggcccgaa gcgcctcctt agtccctgtc ttgccaacgc
     6061 tccccgtcac ggcgaccacg cgtgcggctg tccgcgcacg ggcggcgcgg gcgatggcct
     6121 gtaacgccga gagcgtttca tcgactgtta gaagcgcgcc gtctcccggc tggcggcgat
     6181 aggagcgcga gacaaccgct gcgctggcac ccgcagcgaa cgcggccggg acgaattcgt
     6241 ggccgtcgcg gacgtccttc agcgcgatga acaagtcatt tttctgcagg gtgcggctgt
     6301 cgatcgacac gc
//
LOCUS       DASUMG010000464         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_383099, whole genome shotgun sequence.
ACCESSION   DASUMG010000464 DASUMG010000000
VERSION     DASUMG010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383099"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFX46_07640"
     CDS             <1..403
                     /locus_tag="VFX46_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX5600492.1"
                     /translation="EQNRKKRPVIVWIHGGAFVGGASSLYRLDRLAREGDVVVVSMNY
                     FFPNFSNTSKMDAPDLSPASQALADQMVAYWTSFAWHGKPTAPNSPVWETFNTTKSTL
                     RFEPGKVGGFDAWEAHNCGFWADLYPSILKD"
     gene            complement(408..1022)
                     /locus_tag="VFX46_07645"
     CDS             complement(408..1022)
                     /locus_tag="VFX46_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX5600493.1"
                     /translation="MDPSSLIIFAGALLVAAASPGPGVAAITARVLGRGMQGAFAFTA
                     GVATADVVWLTVAALGLSAIAQTFTGVFLLIKYVGAAYLLYLAWRMWTAPAVVHDLSA
                     KQPDDKPLRLYLAGLAVNIGNPKAALFYLALLPTIIETATINLLGWLELTIVTLAVLA
                     VVFGSYILLAARTRALFTTPRAMRLVNRGSGILMASAAAAVASR"
     gene            1247..2344
                     /gene="ychF"
                     /locus_tag="VFX46_07650"
     CDS             1247..2344
                     /gene="ychF"
                     /locus_tag="VFX46_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5600494.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTQTAAAEAANYPFCTIEPNVGE
                     VGVPDPRLEKLAAIAKSAQTIPTRLTFVDIAGLVRGASKGEGLGNQFLAHIREVDAVA
                     YVLRCFEDPDIAHVEGRIDPLGDADVVETELMLADLESLERRRIPLEKKAKSGEKEAK
                     LQLAVIDKALALLRDGKPARLAELTAEEEPLFKQLQLLTAKPVLYVCNVDEASAASGN
                     DYSRLVEERAKAEGAGCVVISAKIEAELAGLSPEDRQAYLAELGLEEPGLNRLIREGY
                     RLLGLITYFTVGPKETRAWTVPAGTKAPKAAGVIHTDFEKGFIRAETIAYDDFIAYGG
                     EAGAREAGKMRLEGKDYVVKDGDVLHFRFAT"
BASE COUNT          481 a          746 c          799 g          421 t
ORIGIN      
        1 cgaacaaaac cgaaaaaagc ggccggtcat cgtatggatt catggcggcg cgttcgtcgg
       61 cggggcgagc tcgctctacc ggcttgatcg cctggcccgc gagggggatg tcgtcgtggt
      121 ttcgatgaac tactttttcc cgaacttctc gaacacctcg aagatggatg cgccggacct
      181 gtcaccggct tcgcaagccc tggccgatca gatggtcgcc tactggacca gctttgcctg
      241 gcatgggaag cccacggccc cgaattctcc ggtgtgggag acgttcaaca ccaccaagtc
      301 gacgctccgg ttcgagccgg gcaaggtcgg tggattcgat gcctgggaag cgcacaactg
      361 cggcttctgg gccgatctct atccgtccat cctgaaggac tgatgtctta gcggctggca
      421 acggcagcgg cggcggaggc catgaggatt ccgctgccgc ggttgaccag ccgcatcgcc
      481 cgcggcgtgg tgaacaaggc acgcgtgcgg gcggcgagaa gaatgtaact gccgaaaacg
      541 acggcgagca cagccagcgt cacaatcgtc agctcaagcc aaccgagcaa gttgatggtg
      601 gcggtctcga tgatggtcgg caggagtgcg aggtagaaca acgccgcttt tggattgccg
      661 atgttcaccg ccagaccagc gaggtagagc cgcagcggct tatcatccgg ctgcttcgcc
      721 gagaggtcat ggaccacagc tggcgccgtc cacatccgcc aggcgagata aagcaggtag
      781 gccgctccca cgtacttgat cagcaggaat acgcccgtga atgtctgggc gatggcggat
      841 agcccaagcg ccgccactgt gagccagacg acatcggcgg ttgcaacgcc agccgtgaag
      901 gcaaatgcgc cctgcatccc gcggcccagc acgcgcgcag taatggcggc aacgcccggt
      961 cctggagatg cggccgcgac aagcaacgca cccgcgaaga tgatgagcga gctcggatcc
     1021 atcgggcgta ccgttcaatt caggagggtg agagatttcg tccaatcctg gagtatcagg
     1081 tcagcatgca tctgagaata ggcaagtgca gtcgtcacgc agctcttgta gccgtgccgg
     1141 gcgccatgcg gcgccaggag gggttttcaa gatgactccc agggcttaga tgctgagtgc
     1201 ctgaccgggg ccaccgggca ggcttccccg gacaggattg gacttcatgg gctttaaatg
     1261 cggcatcgtg ggcctgccga atgtcggcaa gtccactctc ttcaacgcgc tgactcaaac
     1321 cgcggctgcg gaagccgcca actacccgtt ctgcacgatt gagccgaacg tgggcgaggt
     1381 tggcgttcca gacccgcgcc tcgaaaagct ggcggcgatc gcgaagagcg ctcagaccat
     1441 tccgacgcgg ctcaccttcg tggacatcgc cggtctggtg cgtggcgcct ccaaaggaga
     1501 ggggctcggc aaccagtttc tggcgcatat ccgcgaggtg gacgcggttg cctacgtgct
     1561 gcgctgcttc gaggatccag acattgccca tgtggaaggc cgcatcgatc cactgggcga
     1621 tgccgacgtc gtcgagacgg agctgatgct cgccgatttg gaaagcctcg agcgccgccg
     1681 cattcccctg gaaaagaagg ccaaatccgg ggagaaggaa gccaagctcc agcttgcagt
     1741 catcgacaag gcgctggcgc tcctccgcga cggtaagccc gcgcgtctgg cggagctgac
     1801 ggctgaggaa gagccgcttt tcaagcagct ccagctcctg acagcaaagc cagtgcttta
     1861 cgtctgcaac gtcgatgaag cctcggccgc gtcgggcaac gactactcac gcctggtcga
     1921 ggagcgtgcc aaggccgaag gggccggctg cgtcgtcatc tccgcgaaga tcgaagcaga
     1981 gcttgctggc ctttcgccgg aggaccggca ggcctatctc gccgagcttg ggctcgagga
     2041 gccgggtctc aacaggctga tccgtgaagg ctaccggctg ctcggcctta tcacgtactt
     2101 caccgtcgga cccaaggaga cgcgcgcctg gactgttcca gccggcacca aggcgccgaa
     2161 ggccgccggc gtcatccata ctgacttcga gaaaggcttc attcgcgccg agaccatcgc
     2221 ctatgacgac ttcattgcct acggcgggga ggccggggcg cgggaagctg gcaagatgag
     2281 gctggaaggc aaggattacg tcgtcaagga cggcgacgtg ctgcacttcc gcttcgcgac
     2341 ctgaggcaag actaggtccg tcaggatgcg ggctttttct gggacttgcc ctcgggcttc
     2401 gccagcgcgc gcttggcccc tcgcggcagc gaaagcggcg gcgtcac
//
LOCUS       DASUMG010000465         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1026107, whole genome shotgun sequence.
ACCESSION   DASUMG010000465 DASUMG010000000
VERSION     DASUMG010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026107"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1790
                     /locus_tag="VFX46_07655"
     CDS             282..1790
                     /locus_tag="VFX46_07655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5600495.1"
                     /translation="MPTKTFAELGLSSKVLAAVEAAGYTSPTPIQAEAIPVALTGQDV
                     LGIAQTGTGKTASFVLPMITRLETGRARARMPRSLILAPTRELAAQVAQSFEKYGVNH
                     KLTVALLIGGVSMDDQTRKLDRGVDVLIATPGRLLDHFQRGRVMLMGVEILVIDEADR
                     MLDMGFIPDIEKICKLLPPKRQTLFFSATMPPEIQRLVDNFLTNPARIEVAPPATAAK
                     AITQRFVFCRDAEDRAKREQLRQLIRSQDVRNAIIFCNRKRDVAILNRSLLKHGFNAG
                     ALHGDMDQKHRMETLDKFRSGEITVLAASDVAARGLDIPEVSHVFNFDVPWAADDYVH
                     RIGRTGRAGREGFAATLVTREELKLVKQIEQVTGEQPIWIGEPPTEEDYAEATRSKRR
                     RRRGGKVEPRSATANPERKSKQERPRREKKSAPPTEAASSPVSQVAGEAIPAQEKTRT
                     SGKPRPVKTRSAPDRELRPSREPHPETAGVGFADKIPAFMRRPVRIAKAANE"
     gene            complement(1816..2178)
                     /locus_tag="VFX46_07660"
     CDS             complement(1816..2178)
                     /locus_tag="VFX46_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX5600496.1"
                     /translation="MASSDEFLELLRDLLNGLGPIRIRRMFGGAGIYCDGMMFALVSD
                     DTLYLKADDVNRGNFEARSLAPFRYETKDGRMAVMSYWRAPEELLDDPDELMVWARAA
                     LEAARRAQLNKAKRMRSG"
     gene            complement(2193..>2539)
                     /locus_tag="VFX46_07665"
     CDS             complement(2193..>2539)
                     /locus_tag="VFX46_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX5600497.1"
                     /translation="FPRNMFRGTAEAFATDNKALEVARGAIERGFDKVFDKDGRAFMY
                     LPFEHSENLADQERAVELFTPLDDAEYTHYAIAHRDIIARFGRFPHRNAILGRTSTPE
                     EEEFLKQPGSSF"
BASE COUNT          505 a          810 c          765 g          459 t
ORIGIN      
        1 ccgcagcaca actcgtggta aagtgtggca gctgagtgcc tcccttcgcg cctgcgactc
       61 tgacacgcgg tccgaaggca cggtttttct tgcgttcgac aaccatatat gtcagcaggg
      121 gctgtgctga gcgcagggga tgtattcatt agtccctttt ttgcacacgc ttcatttccg
      181 ttttgccact ttggcgatcc cgaaatcccc ctgcgctcga tcggccgctg ctctctggag
      241 caccgcggcc catgtgaacg cagatgagga acgaggattt tttgccgacg aaaacatttg
      301 ctgagctcgg actcagctca aaggttctgg ccgccgtcga ggcagccggc tacaccagcc
      361 caactcccat tcaggccgag gccatcccgg tcgccttgac cggtcaggat gtgctcggaa
      421 ttgcccagac gggcaccggc aagacggcat cgttcgtgct tccgatgatt acgcggctgg
      481 aaaccggccg ggctcgggcg agaatgcccc gatccttgat cctggctcca acacgcgagc
      541 tggcagctca ggttgcgcag agcttcgaga agtacggcgt aaaccataag ctgaccgtcg
      601 cactgctgat cggcggcgtg tcgatggacg atcagacccg taagctcgac cgcggcgtcg
      661 acgtgctgat cgccactccg ggccgcctcc tcgatcactt ccagcgcggt cgcgtaatgc
      721 tcatgggagt cgagatcctg gtcatcgacg aagccgaccg aatgctcgac atggggttca
      781 tcccggacat cgaaaaaatc tgcaagctat tgccgccgaa gcggcagacg ctcttctttt
      841 cagcgaccat gcctcccgaa attcagcggc tggtcgacaa ctttcttacc aaccccgcac
      901 gcatcgaagt tgccccgccg gccacggccg cgaaggccat cacgcagcgt ttcgtcttct
      961 gccgagacgc tgaggatcgc gccaaacgcg agcagcttcg ccagctcata cgcagccagg
     1021 acgtgcgcaa cgccatcatc ttctgcaacc ggaagcgcga tgtcgccatc ctgaacaggt
     1081 cgctactgaa gcacggcttc aatgccggcg cgctccacgg cgacatggac cagaagcacc
     1141 ggatggagac gctggacaag ttccggtcgg gcgagatcac cgtgctagcc gcgagcgatg
     1201 ttgcggcgcg cggcctcgat attcccgagg tgagccatgt gttcaacttc gacgtgccgt
     1261 gggccgcaga tgactatgtc caccgcattg gccgcaccgg ccgcgccggc cgggagggct
     1321 ttgcggcgac cttggtgaca cgtgaagagc tcaagctcgt gaagcagatc gagcaggtca
     1381 cgggcgagca gccgatctgg atcggcgagc ctccaaccga agaggattat gcggaggcaa
     1441 ctcgcagcaa gcggcgcagg cgccgtggcg gcaaagtaga gccacggagc gcaaccgcga
     1501 atcccgagcg caagtccaag caggaacgtc cgcgacgcga gaagaagtcc gcaccgccta
     1561 cagaggctgc gtcatctccg gtttcccaag ttgctggcga agctatcccg gctcaagaga
     1621 agacccgcac cagcggcaaa ccgcggcccg tgaagacccg ttccgcacca gatcgagagt
     1681 tgcgtccatc gcgagagcct cacccggaga cggcaggcgt cggtttcgcg gacaagatcc
     1741 cagcgttcat gcgccggccg gtccgtatcg caaaagcggc gaacgagtaa gcctcagaca
     1801 gacagaagtt gggctttagc cggagcgcat acgcttcgct ttgttgagtt gcgcccgacg
     1861 agctgcctca agcgcagccc gcgcccaaac cattagctca tcaggatcat cgagcagctc
     1921 ttctggtgct cgccagtagg acatgacggc catgcggccg tccttcgtct cgtagcggaa
     1981 cggtgcgagc gaccgcgcct cgaaatttcc gcggttcaca tcgtcggcct tcagatagag
     2041 cgtgtcatcc gacacgagcg cgaacatcat gccatcgcag tagatgccgg cgccgccgaa
     2101 catgcgccgg atgcggatcg gccctagccc attcagcaga tcgcgcagca gctccagaaa
     2161 ctcgtcactg ctggccacac gtgcttcccg actcagaatg agctgccggg ctgctttagg
     2221 aactcttcct cttcgggagt cgaggtgcgg ccgagaatag cgtttcggtg cggaaagcgg
     2281 ccgaagcggg cgatgatgtc cctgtgcgcg atggcatagt gggtgtattc ggcatcgtcg
     2341 agcggcgtga agagctcgac ggcacgctcc tggtcggcca aattctcgct gtgctcgaac
     2401 ggcaggtaca tgaacgcccg gccgtccttg tcgaagacct tatcgaaccc gcgctcgatc
     2461 gcaccgcggg ctacctccag cgccttgttg tccgtcgcaa acgcttccgc ggtgccgcgg
     2521 aacatgttcc ttgggaact
//
LOCUS       DASUMG010000466         4608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_784335, whole genome shotgun sequence.
ACCESSION   DASUMG010000466 DASUMG010000000
VERSION     DASUMG010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784335"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VFX46_07670"
     CDS             <1..952
                     /locus_tag="VFX46_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX5600498.1"
                     /translation="GGSVRARFPHKDFGKPGILICGHMDTVHPIGTLKVNPWRIENGR
                     CYGPGIQDMKGGNYVSLEAIRQIARAGLETPLPVTFLLTPDEEVGTPSTRELIETEAR
                     KNKYVLIPEPARQDGGAVTGRYAIARFNIRTIGRPSHAGYLLKEGRSAIATMARKILE
                     IEAMTTDDCTFSVGVIHAGKWVNCVSSLCEAEVLSMAKRQEDLDRGVERMLALNGEEN
                     EVTIEVTRGVTRPVWEPDEGTMALYEIARDIAREIGFELTHGSAGGGSDGNFTGALGI
                     PTLDSIGVRGAGLHTPGEHIEIDSLVERARLIAGLLLRLN"
     gene            1137..2012
                     /locus_tag="VFX46_07675"
     CDS             1137..2012
                     /locus_tag="VFX46_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEX5600499.1"
                     /translation="MTNTPETSVPILSGAGRLLSQYDAIFCDVWGVLHNGVQPYAAAG
                     EALARFRAAEGVVVLVSNAPSPSEMVAKMLDRIGVRRDAWDAIITSGDLTRAYLQERQ
                     YRFIHHIGSPRDMRIFAGLPVEHVALERAAAIVCTGVADVSQTAEIYRPILARGIELA
                     LPFVCGNPDLVVEIGGKLLPCAGAVATLYEQMGGDVYWAGKPHAPAYAAAHQLAERVR
                     NRPIERKRILAIGDAVRTDLVGAANYGVDCLLVAHGIHRDELLRGHEVHSERLEQLLQ
                     STPHRPIAAMAALAW"
     gene            2111..3274
                     /locus_tag="VFX46_07680"
     CDS             2111..3274
                     /locus_tag="VFX46_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEX5600500.1"
                     /translation="MTLREPSFTFGIEEEYHLVDRSTRDLAAAPQELMDECLAQLGTQ
                     VSPEFLKSQIEIGTPVCRSFAETRKHLAELRGTIGQIAGRFGMAPIAASTHPFAHSLQ
                     QTTTDKERYQALAQDLQGVGRRLVVCGMHVHVGIEDQELRIDIMNQVRYFLPHLLALS
                     TSSPFWEGQNTGLKSYRLAIMQGLPRTGMPGRLSGWDEYQRIVNVLVNAGVIEDDTKI
                     WWDVRPSHRFPTLEMRITDVCTRLDDAVSVAALFLCICRMLYRLRRNNQNWRMYPVFL
                     VNENRWRAQRYGVEGTLFDFGKGTLEPFADLLGEIQALVSEDAAALGCKAEVAHASRI
                     VAEGTSADRQVAIYERLISGGASEEVALRGVVDNLIEETLAGTTFLGAPQQLA"
     gene            complement(3297..4001)
                     /locus_tag="VFX46_07685"
     CDS             complement(3297..4001)
                     /locus_tag="VFX46_07685"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX5600501.1"
                     /translation="MACLLRRHRLRCSAQALGLIVLFLLFLSPQVQADTWEFDKQNSS
                     IRFTWDHLGLSRQSGRFLDFDGRLHFSPTDPEGGSVDVVIKAASLWTGVREYDENLKS
                     DSFFNVDRYPIITFRSTWVRRTGERTGEVDGELTIMGISRPVTLKVIWNYTGEHPFAN
                     VNPTYKGKWVSGFSATTKVKRSEWGLTRALPLISDDIWITIEAEFLLKDENRVGAPLR
                     GGPRLESQSPYTFIPR"
     gene            complement(4099..>4608)
                     /locus_tag="VFX46_07690"
     CDS             complement(4099..>4608)
                     /locus_tag="VFX46_07690"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5600502.1"
                     /translation="RLDVLVNNAGLNVRGDFRHISEADWVRVREVNLDGVVRVAREGF
                     NLLRVSGNSSLINLSSIMASRGLRQLASYSATKGAVSALTRALAVEFAPFGIRVNALA
                     PGFIETALTERVLRNPVINKALLDRTPLRRFGTADDVANAALFFASDESAFITGAELA
                     IDGGMSASL"
BASE COUNT          892 a         1401 c         1417 g          898 t
ORIGIN      
        1 cggtggctcg gtgcgtgccc ggtttccgca taaggatttc gggaaacctg gcattctgat
       61 ttgcggccac atggatacgg tgcacccgat cggcacgctc aaagtcaacc catggcgcat
      121 cgagaacggg cgctgctacg gtcccggcat tcaggatatg aagggcggaa actacgtcag
      181 cctcgaagcc atccggcaga ttgcgcgcgc cggtctggag acgccgttgc ccgtcacatt
      241 tctcctcacg cctgacgagg aggtcggcac accgtccacg cgcgagctca tcgaaactga
      301 agcgcgcaag aacaaatacg tcctgatccc cgaacctgca cggcaggatg gcggtgccgt
      361 aaccggccgc tatgcgatcg cacgcttcaa catacgaacc attgggcgac ccagtcacgc
      421 cggctacttg ctgaaggaag ggcgctctgc catcgcgacc atggcccgca aaattctaga
      481 gatcgaagcc atgaccacgg acgattgcac cttcagcgtc ggggtcattc acgcgggcaa
      541 atgggtgaac tgcgtctcct ccctctgtga ggccgaggtg ctcagcatgg ccaaacgcca
      601 ggaggacctg gatcgcggtg tcgaacgcat gctcgcgctt aatggcgagg aaaacgaggt
      661 aaccatcgag gtcacgcgcg gcgtcacgcg cccagtctgg gagccggatg aaggcacgat
      721 ggcgctttac gaaatcgcgc gggacatcgc ccgggaaatc ggcttcgagc tcacgcacgg
      781 aagcgcgggt ggcggatctg acggcaactt taccggcgct ctcggcatcc cgaccctaga
      841 ctcgattggc gttcgtggcg caggacttca tacgcccggc gaacacattg agatcgatag
      901 tctcgtggag cgtgcgcgcc taatcgccgg gctgctgctg cggctgaact gagctggaaa
      961 tacgatgagc atcgctcgca gcgtcagcaa tccaactcgc atttgagagg cagggccatt
     1021 catatgctct gtctcgccga tgtttgtctg tgagttcgag ttccgctatg ggggtgttcg
     1081 tcaagtgtca ggagcgcccg catcaagaaa tgcctcctgc tcaccgaaag ggagatatga
     1141 cgaacacacc ggaaacctcg gttccgatcc ttagcggtgc tgggcgattg ctctcccagt
     1201 atgacgccat tttctgcgac gtttggggcg tccttcacaa tggtgttcag ccttatgccg
     1261 ctgccggaga ggcactcgcc cggtttcggg ccgctgaagg cgttgtcgtg ctcgtctcga
     1321 acgcaccgtc cccttcggaa atggtcgcga aaatgctcga tcgcatcggc gttcgacgcg
     1381 atgcatggga cgcgatcatt acctccggtg atcttacgcg cgcctatctc caggagcgcc
     1441 agtaccggtt cattcatcac atcggatcac cgcgtgacat gcgcattttc gcgggtctcc
     1501 cggtcgagca cgtcgctctc gagcgggcgg cggccattgt ctgcacgggc gtcgcagatg
     1561 tgagccaaac agccgaaatt tatcggccta ttctggctcg gggtatcgag ctggcgctgc
     1621 cgttcgtctg cggcaatccc gatcttgttg tcgagattgg cggcaagttg ctgccctgcg
     1681 caggtgccgt cgctacgctc tacgaacaga tgggcggtga cgtgtattgg gctggaaagc
     1741 ctcatgctcc agcttatgcc gctgcgcatc agcttgccga gcgtgttcgc aacaggccga
     1801 tcgagcgaaa acgtattctg gccattggcg atgcggtccg cacagacttg gttggcgccg
     1861 ccaattacgg ggttgattgc cttctggttg ctcacggcat ccaccgcgat gagctgttgc
     1921 gggggcatga ggtccactcg gaacgcctcg agcaactgct gcaatcgacg ccgcacaggc
     1981 ctattgccgc gatggcggct ctcgcctggt aatgctgtcg cgatactgaa aggcgcagtt
     2041 gcgcgtttct ctgtcgtctc ggcaccgttg agctggtgtg ccgggtgggc ggctgtgaga
     2101 tgatgaatcc atgacgctga gagaaccttc gttcaccttc ggcatcgagg aggaatatca
     2161 cctcgtcgat cgaagcacgc gcgacctcgc ggcagcgccg caggagctga tggacgaatg
     2221 tcttgcccag ctgggcactc aggtcagccc tgagttcctc aagtcacaaa ttgaaatcgg
     2281 cacgcctgtg tgccgctctt tcgccgaaac acgaaaacat ctcgcggagc tgcgtggcac
     2341 cattggccag atcgccggcc ggttcgggat ggcaccgatc gcggcctcca cgcatccgtt
     2401 tgcccactcc ctgcaacaga cgacaacaga caaagaacgc taccaggcgc tggcgcaaga
     2461 cctccaggga gtgggccggc gcctcgtggt gtgcggaatg cacgtgcacg tcggaatcga
     2521 ggatcaagag ctgcgcatcg atatcatgaa tcaggtgcgc tatttcctgc cgcacctgct
     2581 cgccctttcg acatcctcac ccttctggga gggccagaac accgggctca agagctaccg
     2641 cctagcgatc atgcaaggcc taccccgaac gggcatgcct ggccgccttt ccggatggga
     2701 cgagtaccag cggatcgtga acgttctggt caatgcaggc gtcatcgagg atgacaccaa
     2761 gatctggtgg gacgtgcggc cttcgcatcg ctttcccacc ctggaaatgc ggattaccga
     2821 cgtctgcacc cggctcgatg atgctgtttc ggtcgccgcg ctattcctct gcatctgccg
     2881 catgctctac cgtttgcgcc ggaataacca gaactggcgg atgtatcccg tcttcctcgt
     2941 caacgaaaac cgctggcgtg cgcagcggta cggcgtcgag ggcacgctgt tcgacttcgg
     3001 caaaggcacg ctcgagccgt ttgccgacct gctaggcgaa atacaggccc tcgtctcgga
     3061 ggatgctgct gcgttgggct gcaaggcgga ggtggcgcat gccagccgga ttgtggccga
     3121 aggcaccagc gccgaccggc aagtggcgat ctatgaacgc ctgatcagcg gcggcgcgag
     3181 cgaggaggtc gccttaagag gtgtcgtcga taacctcatc gaggagaccc tggctggcac
     3241 cactttcctc ggtgctcccc agcagctcgc ttgagcgatc gcgtcctcgc cctcggttac
     3301 ctcggtatga acgtgtacgg agactgcgac tccagacgcg ggccgcctcg caaaggagcc
     3361 ccgacgcggt tttcgtcctt cagcaggaac tccgcttcga ttgtgatcca gatatcatcc
     3421 gagatcagcg ggagggcgcg ggtcagtccc cattcgctgc gtttcacttt cgtggtggcg
     3481 gaaaagcccg agacccattt gcccttgtag gttggattca cgttggcgaa tggatgctcg
     3541 ccggtgtagt tccagatcac cttcagggtg accggccggg agatccccat gatggtaagc
     3601 tcgccgtcca cctcccctgt tcgttcgcct gtgcgccgaa cccaggtgct ccgaaacgtg
     3661 atgatggggt agcgatcgac gttgaagaag ctgtcgctct tcaggttctc gtcgtactcc
     3721 ctgacacccg tccagagact tgccgctttg atgaccacat cgacggaccc gccttccggg
     3781 tcggtcggtg agaagtggag ccggccgtcg aaatccagaa acctgcccga ttggcgcgac
     3841 aggccaaggt gatcccaggt gaaccggatc gatgagttct gcttgtcgaa ctcccacgtg
     3901 tcagcctgaa cctgcggcga cagaaaaagg agaaagagga cgatcaggcc gagcgcttgc
     3961 gctgagcagc gcaaccggtg cctgcgcagg aggcaagcca ttggctttca aactcctgaa
     4021 ttgatgggca ttggcagccc gcagtttgcg tctgctttcg gaacaccagc acaatgtgac
     4081 ctgaagttgt cgggcggctc acaaactcgc cgacatgccg ccgtcaatcg ccagctcggc
     4141 gcctgtgatg aaggcgctct catcggaagc gaagaacaat gcggcgttcg cgacgtcatc
     4201 cgccgtgccg aaccgcctga gcggggtgcg atcgagaagc gccttgttga tcaccggatt
     4261 gcggagcacg cgctccgtca atgccgtctc gatgaagccg ggagcaagcg cgttcacgcg
     4321 aatgccgaac ggagcgaact cgaccgcgag cgcgcgcgtc agggcggaaa cggcgccctt
     4381 ggtggccgag tagctcgcca actggcgcaa gccacggctc gccatgatgg atgaaaggtt
     4441 gatcagcgag ctgttgcccg agactcgcag aaggttgaac ccctcgcgcg cgacccgcac
     4501 gacgccatcg agattcactt cccgaacgcg aacccagtca gcctcggaga tgtggcgaaa
     4561 atctccgcgg acgttgaggc cggcgttgtt tacgagcacg tcgagccg
//
LOCUS       DASUMG010000467         2318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_945360, whole genome shotgun sequence.
ACCESSION   DASUMG010000467 DASUMG010000000
VERSION     DASUMG010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_945360"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /gene="fadE"
                     /locus_tag="VFX46_07695"
     CDS             complement(<1..350)
                     /gene="fadE"
                     /locus_tag="VFX46_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX5600503.1"
                     /translation="MSQLIMALVAIAVVFLLAMRRASLPVWAGAVTALTFVWQTGLVD
                     GVPAPFGLWSLIGWLPAIALAVLSVPEVRRSLIVEPAYRAIRSSLPKISETEQQALEA
                     GTIGFDAELFSGRP"
     gene            662..1018
                     /locus_tag="VFX46_07700"
     CDS             662..1018
                     /locus_tag="VFX46_07700"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5600504.1"
                     /translation="MAQDFGVAGDQLVPSGKILADMQAAAVEAAKMLRSIGSPYRLMI
                     LCLLMESEKTVSEISEAIGARQSLTSQHLTHLRLHGLVHVERRGHFAYYSLADTVAKD
                     IVAVLYAHYCADKMQR"
     gene            1083..1406
                     /locus_tag="VFX46_07705"
     CDS             1083..1406
                     /locus_tag="VFX46_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX5600505.1"
                     /translation="MVGWFAAGGVALGIFAAAGLSQAKSTEYLMKLGEHLAQECTSCH
                     RIDGTDNGIPSIVGLDPEYFINSLTYYKDRERTNPIMVSVAESLDKEQIEALALYFHS
                     LGKKK"
     gene            complement(1438..>2318)
                     /locus_tag="VFX46_07710"
     CDS             complement(1438..>2318)
                     /locus_tag="VFX46_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600506.1"
                     /translation="ARIWNELKPPVERAPVPSVADVLESAKTVYGFEPERIPEKEFKR
                     RYALEALTHGLTKEQVVRVYALETGGRGTHDMQAGINPQTGKGTPISTALGYAQLLHA
                     NSVNELVKHGNTFISRLEKTAEMPGIAPARAAALQKKARAVRRMLASAKTVPNDWYAH
                     MKFAGTRNGLGIHALNLDGDIGPMLQVIKLKGIREVAERAGRTNLTPAELELMNLAGP
                     STGLEMMTPVGRTAPTPNFFSRLGYERNPVVHKRTAAELLARIDEIMDANVSKPGSVE
                     FAAAFDEAASFRASQR"
BASE COUNT          442 a          723 c          708 g          445 t
ORIGIN      
        1 tcagggcggc cggagaacag ttcggcatcg aacccgatcg tgccagcctc cagggcctgc
       61 tgctctgtct cggagatctt cggcaaggag ctgcggatcg cgcggtacgc cggctcgacg
      121 ataaggctgc ggcgcacttc ggggacggac aggacagcaa gcgcgatggc cggcagccac
      181 ccgatcaggc tccagagccc gaacggagcc ggcaccccat cgacaagccc ggtctgccaa
      241 acgaatgtga gcgccgtcac cgcccctgcc cagacaggca agctcgctcg ccgcatggcg
      301 agcaggaaga cgaccgcaat ggcgacgagg gccatgatca gttgcgacat cggttggcct
      361 ccgttccttc ctcgggctgc ctagtgcgcg acccgcaaac tgcgccagag cgttctagaa
      421 cggcacgcgc gaagatcagc ttaacgccct ggccgagacc tgcctcgggc gggaaactta
      481 cgtgcgctcg gcgtcgatcg tgctcgaata taaggcccct tgctgtcctg cgcgaggcgt
      541 gacagcgcat tgtcgccgga gcatgcctga agtcaggccc ggtgtggtca tgcgccttca
      601 ttgccaaggg ggcctcaggg caatatccta ctgccaatca agactttcag gatcgagttg
      661 catggcgcag gatttcggcg ttgccggcga tcagcttgtc ccgagcggga agattctcgc
      721 cgacatgcag gcggctgcag tcgaggcggc gaagatgctg cgctcgattg gcagtcctta
      781 ccgcctcatg atcctctgcc tcttgatgga gagtgagaag acggtcagcg agatttcaga
      841 agccatcggg gcccggcaaa gcctcacctc acagcatctc acgcacttgc ggctgcacgg
      901 gctcgttcac gtcgagcggc gcgggcattt cgcctactac tccctggcag acacggtggc
      961 caaggacatc gtggcagtcc tttacgccca ctattgcgcc gacaagatgc agcgctagaa
     1021 tccccccgga tcgatccgtc ggccccaacg ccggccgaca cgcctttggg acggagggac
     1081 gcatggtggg ctggttcgcg gcgggaggcg tagctctggg cattttcgca gcagccggat
     1141 taagccaagc caaatcgacg gaatatttga tgaagctcgg agagcacctg gcccaggagt
     1201 gcacgagctg tcaccgcatt gatggcaccg acaacggtat tccgtcgatc gttgggctcg
     1261 atccggagta tttcatcaac tcgcttacgt attacaaaga tcgtgaacgg accaatccca
     1321 tcatggtctc ggttgctgag tcgctcgaca aggagcagat cgaggcactc gcgctctatt
     1381 tccactcgct tggaaaaaag aaatagcgaa gcgcgttagc gcggaaaaat ttgcgcgtta
     1441 tcgctgagaa gctcgaaagg acgccgcctc gtcaaacgca gcagcgaact cgaccgagcc
     1501 cggcttactc acgttcgcgt ccatgatctc atcgatacgc gccagtagct cggctgcggt
     1561 gcgtttgtgc acaacgggat ttcgctcgta gccaagccga gagaagaagt tcggcgttgg
     1621 cgccgtgcgg cccacgggag tcatcatctc gagccccgtg ctcggaccag cgagattcat
     1681 cagctccaac tcggctggcg tgagatttgt ccttccagcg cgctcggcaa cctcgcgaat
     1741 gcctttcaat ttgatcacct gcaacatcgg gccgatgtcg ccatcgaggt tgagcgcgtg
     1801 aatgccgagc ccgttgcgag tgcccgcaaa cttcatgtgc gcgtaccagt cgttgggtac
     1861 ggttttcgcg ctcgccagca tacgtctcac agcgcgggcc ttcttttgca aggcggcagc
     1921 ccgggcaggg gcgatgcctg gcatctcggc cgttttctcg agccgtgaga tgaacgtatt
     1981 cccgtgcttg accagctcgt tgaccgaatt cgcatgaaga agctgtgcgt agcccaacgc
     2041 tgtcgatatc ggcgtgcctt ttccggtctg cggattgatt ccagcctgca tgtcgtgggt
     2101 gccccggccg cccgtttcca gggcgtagac acgcaccacc tgctctttgg tgagcccatg
     2161 agtcagcgcc tccagcgcat agcgccgctt gaactccttc tcagggatgc gctccggctc
     2221 aaatccgtag acggtcttcg cgctttcgag cacatcggcg acgctgggca cgggcgcccg
     2281 ctcgaccggc ggcttcagct cgttccagat gcgggcga
//
LOCUS       DASUMG010000468         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_784719, whole genome shotgun sequence.
ACCESSION   DASUMG010000468 DASUMG010000000
VERSION     DASUMG010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784719"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFX46_07715"
     CDS             complement(<1..329)
                     /locus_tag="VFX46_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX5600507.1"
                     /translation="MRKLEKGTKLVVASHNPGKLREIADLVEPYGLEVVSAAELALSE
                     PEETETTFAGNARLKAVTAAKAAGLPALSDDSGLEVEALGGQPGVYSARWAGPEKDFN
                     LAMKRVA"
     gene            complement(329..736)
                     /locus_tag="VFX46_07720"
     CDS             complement(329..736)
                     /locus_tag="VFX46_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5600508.1"
                     /translation="MKPAGILETVLYASDLDAAERFYRDVLGMEPFSKSPGRQLFYRC
                     GDQVFLVFNPEATSKPPAPNALPVPPHGAVGPGHVCFRANAAEIEVWRKHLESHGIAI
                     EADFQWPGGRSIYFRDPAGNCLEFAEPLIWGLE"
     gene            complement(783..1496)
                     /gene="rph"
                     /locus_tag="VFX46_07725"
     CDS             complement(783..1496)
                     /gene="rph"
                     /locus_tag="VFX46_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX5600509.1"
                     /translation="MRPSKRKPDELRRVSFERAVSMYAEGSCLIKFGHTHVLCTASVE
                     DRVPPWLKNQGRGWVTAEYGMLPRATSERTRRESTAGQQSGRTQEIQRLIGRALRSVV
                     DLQKLGERQITIDCDVLQADGGTRTAAITGAWVALHDCIQWMRARDMIGDGVLKDQVA
                     AVSAGIYAGVPVLDLDYAEDSEAEADANFVMTGSGGIVEVQATAEKEPFSQERFDELM
                     QLARKGIKELIALQQLTVA"
     gene            1674..>2464
                     /gene="hrcA"
                     /locus_tag="VFX46_07730"
     CDS             1674..>2464
                     /gene="hrcA"
                     /locus_tag="VFX46_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX5600510.1"
                     /translation="MTGTISELSQLNERSRTIFRQIVETYLETGEPVGSRNISRNLPM
                     TLSPASVRNVMSDLEQLGLIYAPHTSAGRMPTHLGLRLFVDALLEVGDLTEEERKQIE
                     SQISYKPGKSIEQLLAEASEMISGLSHCAGLVLAEKQVSRLKHIEFVPLEPGRALVVM
                     VGDDQTVENRIISLPPGLPPSALVEAANYLNAHVRGMSIDEARVHIEKQIANARAELD
                     ALTQKVIKAGLAEWSGTLGDRQSLIVRGQSNLLKDLTAIEELERIR"
BASE COUNT          471 a          797 c          744 g          452 t
ORIGIN      
        1 tcggctaccc gcttcatggc caggttgaag tctttttccg gacccgccca acgggctgag
       61 taaacaccgg gctgcccacc gagagcctcc acctcgaggc cggagtcatc agagagggcc
      121 ggcagtccag cggccttagc ggcagtgact gccttcagcc gggcattgcc ggcaaacgtc
      181 gtttccgttt cctcaggctc ggaaagcgcc agctcagcgg ctgagacgac ctctagaccg
      241 tagggctcta cgagatcggc aatttccctc agcttgccgg gattatggct ggcgacgacg
      301 agcttcgttc ccttctcaag ctttcgcatc actcgagccc ccagatgagt ggctcggcga
      361 actcgaggca attgccagcc ggatcacgga aataaataga gcggccgcca ggccactgga
      421 agtcggcctc gatcgcgatg ccgtgggatt ccaagtgctt gcgccaaacc tcgatctccg
      481 cggcgttggc gcgaaaacag acgtgtcccg gccccaccgc gccgtgtgga ggcaccggca
      541 gcgcatttgg cgctggcggc ttcgacgtag cctcgggatt gaacaccaga aagacttggt
      601 ccccgcaccg atagaacagc tgccggcctg gagacttgga gaagggctcc atccccagaa
      661 cgtcgcggta aaagcgctct gcagcgtcga ggtcgctggc atagagcacc gtttccagaa
      721 tcccggccgg cttcatcgct tcacctgcct cgtgcctctc acgatgaccg agctgggatg
      781 gctcaagcca ccgtcaactg ctgcagggct atcaactctt taatgccctt gcgcgccaac
      841 tgcatcagct cgtcgaagcg ttcctggctg aacggctcct tttcggcggt cgcctgcacc
      901 tcgacgatgc cgccggaccc tgtcatgacg aagttggcgt ccgcctcggc ctccgagtcc
      961 tcggcatagt ccaggtcgag aaccggcact cccgcataaa tgccggcgga cacagcagcg
     1021 acctgatcct tgagcacgcc atcgccgatc atgtcacgcg cccgcatcca ctggatgcaa
     1081 tcgtgcaatg cgacccatgc gccggtgatg gccgccgtcc gcgtcccgcc atcggcctgc
     1141 aacacgtcac agtcgatggt gatctggcgc tcgccgagct tctgcaggtc caccactgag
     1201 cgcaaggccc ggccgatcag ccgctggatc tcctgcgtgc gcccggactg ctgaccggcc
     1261 gtgctttccc ggcgcgtgcg ctcgctggtg gcgcggggca gcatgccata ttccgccgtc
     1321 acccaaccac gcccctggtt cttgagccag ggcggcacgc gatcttccac actggccgtg
     1381 cacagcacat gggtatgacc aaacttgatg aggcaggagc cttccgcgta catggacacg
     1441 gctcgctcga atgagacgcg gcgaagctcg tcgggcttgc gttttgacgg tcgcatgaca
     1501 acctttctgg gtgctcaaag ccagccgaga acaggccgac tcaagtgcgc ggcaaactag
     1561 gcgagcgctg gcccgcattc aacagcaatt cgcacgggat ttgacgggtc gcctcggctg
     1621 cgcctacagt tttttaagtc agttgttggt gggagcacct tcaacgagag caaatgacag
     1681 ggaccattag cgagctcagc cagctcaacg agcgctctcg caccattttc cgtcagattg
     1741 tcgagacgta cctcgaaacc ggcgagcctg ttggttcgcg gaacatctcg cgcaatttgc
     1801 ccatgacgct gtctccggca tccgtgcgga acgtgatgtc ggacctcgag cagcttggcc
     1861 tgatctatgc cccccacacg tcggcgggcc gtatgccgac gcacctgggg cttagattgt
     1921 tcgtcgacgc tctgctcgag gtgggcgatc tgacggaaga agagcgtaag caaatcgagt
     1981 cgcaaatctc ctacaagcca ggaaaatcga tcgagcagct cctggctgag gccagcgaga
     2041 tgatctcggg cctttcgcac tgcgccggtc tcgtgctggc cgagaagcag gtgagccggc
     2101 tcaagcacat cgaattcgtg ccgttggagc ctggacgggc gctcgtggtg atggtgggcg
     2161 acgaccagac ggtcgagaac cgcatcattt cgctgccgcc ggggttgccg ccgtccgcgc
     2221 tggtcgaagc cgcgaactac ctcaacgcgc acgtgcgggg aatgtcgatc gacgaagcgc
     2281 gcgttcacat cgagaagcaa atcgccaacg cgcgcgccga gctcgacgcg ctgacgcaaa
     2341 aggtcatcaa ggctggcctt gctgaatggt cgggcacgct tggcgaccga cagagcctga
     2401 tcgtgcgcgg ccaatccaac ctgctcaagg atttgaccgc tatcgaggag ctggagcgaa
     2461 tccg
//
LOCUS       DASUMG010000469         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1026898, whole genome shotgun sequence.
ACCESSION   DASUMG010000469 DASUMG010000000
VERSION     DASUMG010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026898"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFX46_07735"
     CDS             <1..414
                     /locus_tag="VFX46_07735"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX5600511.1"
                     /translation="EIRQILMSKGLTGGALEDAVAAITSNEKVWIETMLAEEYGLSRV
                     LPSPLIAGLYTFWAFVTCGAVPLIPFLLGVQNAFQWAIVATGATFFIIGSIKSRWSLA
                     PWWRSGFETLAIGMSAAGIAYVVGYALRELLGIDV"
     gene            500..2416
                     /locus_tag="VFX46_07740"
     CDS             500..2416
                     /locus_tag="VFX46_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5600512.1"
                     /translation="MTVSGRTSAEAESLRLKAILDSAVTGIITIDSQGRIETVNQAAE
                     RLFGWTASELIGRNVSVLMPEPYASRHDDYIRRYLATGERRVLGIGREVQGLRRDGTV
                     FPMHLSLGEYSIGNEKYFTGIVYDLTEQRSIEARLDREQALSRSIFENLPDALVVSDP
                     ENVIRVVNPAFTRLFGLTEEEVEGQHCSILCEDAEDAKRFTTHADGFGPQQMQFRRRN
                     GSSFPARAVHRPIFGSDGQRIGNLTLIHDITAEREQAAVLQQAQRMEAIGQLTGGVAH
                     DFNNLLTVILGNIELLEPRLEDEFSRSLASEAREAAEMGARLTDRLLTFARRQRLEKR
                     LIDLNEFVLSLTELLRRTIGSDVDLSTSLATDLWLTEADPGQVENAVLNLAINARDAM
                     PNGGRLVIETRNAVLDADAVKLIPDLEPGDYVVLSVADTGIGMTPEVKARAFEPFFTT
                     KGATKGSGLGLSTIYGFARQSGGNVTIYSEPNRGTTVNIYLPRAKQAQKKAEATVGEA
                     VRPSRGETILVVEDDDRVRKLTAVRLEQLGYQVEQAANGQAALALLEQNSGIDLVFSD
                     QVMPGGLSGLDLAAAVRQRFPKVGVVLTSGYSADLIGQEKVDGLGLKVLRKPYQQTEL
                     ARVIREALDSRAAV"
     gene            complement(2546..2752)
                     /locus_tag="VFX46_07745"
     CDS             complement(2546..2752)
                     /locus_tag="VFX46_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600513.1"
                     /translation="MGKRGEKLPIDCVELFAELDNCDDLRDVYARVQDRIRAYSAAGR
                     PVPEALLRCERHLMTEIMAVSQGR"
     gene            3117..>3708
                     /locus_tag="VFX46_07750"
     CDS             3117..>3708
                     /locus_tag="VFX46_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEX5600514.1"
                     /translation="MKIKRRQFLAGVATAGAAAGTAAFPKPAIAQARTEWRMVTTWPK
                     NFPGQGVGAERFAKMVTEATEGRLVIKVYAAGELVPAFESIDAVIRGAADLMHATPYY
                     WPNKSKALNFFSTVPFGMTTPELDAWMNHLGGQELWDKVYDQFGLKAFHAGSTGVQMG
                     GWFNKEITGVDSIKGMKMRIPGIGGEALAKLGMTIIN"
BASE COUNT          735 a         1106 c         1170 g          697 t
ORIGIN      
        1 gagatccgcc agatcctgat gagcaaggga ctcactggcg gggcgctcga ggatgctgtg
       61 gccgccatca cctccaacga gaaggtgtgg atcgagacga tgctggccga ggagtatggc
      121 ctctcccgcg tgctgccgag cccgctgatc gctggccttt acaccttttg ggcgttcgtg
      181 acctgcggag cggtgccgct catcccattc ctcctgggag tgcagaatgc gttccaatgg
      241 gctatcgttg cgacaggcgc gacgttcttc atcatcgggt ccatcaagag ccgctggtcg
      301 ctcgcgccct ggtggcgctc cggctttgag acactcgcga ttggcatgag cgcagccggc
      361 attgcttatg ttgtaggcta cgcgctgcgc gagctcctgg ggattgatgt ctgaggcgga
      421 ttgtcactgc ggcgcctgct tgctcgatcc cagcaggcgt ttgcgcaaaa tcaaaaccaa
      481 aacgagactt ggcaattcca tgacggtatc aggtcgcacg agcgccgagg cagaatcgct
      541 gcggctgaag gccattctcg attctgccgt aaccggaatc atcacgatcg acagccaagg
      601 acggatcgag accgtcaatc aggcagccga gcggctgttc ggctggactg cttccgaatt
      661 gatcggccgc aacgtctcgg tgttgatgcc ggagccgtac gcaagccggc atgacgatta
      721 catccgccgt tatctggcaa ccggcgaacg gagggttctc ggcattggcc gggaggtcca
      781 gggattgcgg cgggacggca cggtcttccc gatgcatctg tcccttggcg agtactcgat
      841 cggcaacgag aagtacttca ccggcatcgt ctacgacctc acggagcagc ggtcgatcga
      901 ggcgcgcctc gaccgggagc aggccctgtc ccgctccatc ttcgaaaacc ttcctgatgc
      961 acttgtcgtc tccgacccgg aaaacgtgat ccgggtcgtc aatccggcct tcacgcgact
     1021 gttcgggctg acggaagagg aggttgaggg ccagcactgc agcatcctgt gcgaggacgc
     1081 ggaggatgcg aagcgcttca ccacgcatgc cgatggtttc ggcccgcagc agatgcagtt
     1141 ccgccgccgg aacggcagct cgtttccggc tcgggctgtg catcggccca tcttcggcag
     1201 cgatggccaa cgcatcggca atctgacgct cattcacgac atcaccgccg agcgtgagca
     1261 ggccgcggtg ctgcagcagg ctcagcgtat ggaggccatt gggcaactca cgggcggcgt
     1321 cgcgcacgat ttcaacaact tgctgacggt catcctgggg aacattgagc tgctcgagcc
     1381 aaggctggag gacgagttca gccgaagcct tgcgtctgag gcccgggagg cggctgaaat
     1441 gggcgctcgt ctgaccgacc gccttttgac gttcgcacgg cggcagcggc tggagaagcg
     1501 cctcatcgat ctcaacgagt tcgtgctgag cttgacggag ctcttgcgcc gcacgatcgg
     1561 gtcggacgtc gacctgtcga cgtcattggc gacggacctg tggttgacgg aggcggaccc
     1621 gggccaggtc gagaacgcgg tgctcaacct cgccatcaat gcgcgggatg ccatgccgaa
     1681 cggcggccgc ctcgtcatcg aaacgcgcaa cgcagtcctc gatgcggacg ccgtcaagct
     1741 catccccgat ctcgagcccg gcgattacgt ggtgctgtcg gtcgccgaca ccggcattgg
     1801 gatgacaccc gaggtgaagg cccgcgcgtt cgaaccgttc ttcacgacga aaggggcaac
     1861 caaggggagc ggtctcggcc tgtcgaccat ttatggcttt gcgcgacaat ccggcggcaa
     1921 tgtcacgatc tatagcgagc caaaccgcgg cacgacggtg aatatttacc tgccgcgagc
     1981 gaagcaggcg cagaaaaagg ccgaggcaac cgttggcgaa gccgttcgac ccagccgagg
     2041 cgagaccatt ttggtggtcg aggatgacga ccgggtgcgg aaactcacgg ccgttcggct
     2101 ggagcagctt ggttaccagg ttgagcaggc ggcgaacggg caagcagccc tggctctgct
     2161 tgagcagaat tctggcattg atctcgtctt ctccgaccag gtgatgccag gcggattatc
     2221 ggggctcgat ctcgcagcgg cggtgcgtca gaggttcccg aaggtcggag ttgtcctgac
     2281 gtccggctac tcggctgatc tgattggcca ggaaaaggtc gatggcttgg ggctcaaggt
     2341 gcttcgaaag ccctatcagc aaaccgagct cgcccgcgtc atccgcgagg ctctagactc
     2401 ccgcgccgcg gtatgaggcg gcccccgatt cgacggcgag ggacaaaacc cgtaggcgca
     2461 atctgcggaa gcttgcgctg attccgcagg aggatcgcgc ccctaagtcg cctgaatttc
     2521 aaaaattgct tattgcttga ggaagctagc ggccctgcga gacggccatg atctcggtca
     2581 tcaaatggcg ctcgcagcgg agcagagcct cgggcacggg ccgcccggca gccgagtagg
     2641 cgcgaatacg gtcctgcacg cgcgcataca catcccggag gtcgtcgcag ttatcgagtt
     2701 ccgcaaagag ctcgacgcaa tcgattggaa gtttttcgcc acgcttgccc atttggcctc
     2761 tcccaatagc tgcgtttctc tgcccccttg ttggttgctg ccgtgcacca caggtgcagt
     2821 tccttgattg gcagcgaaaa ttggcctttc ggctccgaat ttgtgtgcgg cgcaacaaat
     2881 agtgcgattt ttcgccgttc actgcagata gttaaacgaa ctcggagatc aaggtccagg
     2941 tcgaattcgc caaataggtg caactgcgtc aaagagcgcc ttccgcgctc ggatcaatac
     3001 ggtcgcatgg acaaacgact ggattgcgca acatttacgg cggccacata ctcggccgta
     3061 cggctgagac cagccgaaaa acaatctgaa tggggcagcc caagggagca aatcgtatga
     3121 agatcaaacg ccgccagttt cttgcgggcg tagcgacggc cggcgcggct gctggcacgg
     3181 cagctttccc caaacccgcc attgcgcagg cgaggaccga gtggcgcatg gtaaccacgt
     3241 ggccgaagaa cttccctggc cagggcgtgg gtgccgagcg cttcgcgaaa atggtgacgg
     3301 aagcaactga aggccggctg gtcatcaagg tttatgcagc gggcgaactc gttccggctt
     3361 tcgaaagcat cgatgccgtc atccgcggcg ctgccgacct gatgcacgcc acaccctatt
     3421 actggcccaa caagagcaag gcgctgaact tcttctcaac cgttcctttc ggcatgacga
     3481 cgccggagct cgacgcctgg atgaaccatc tcggcggcca ggagctgtgg gacaaggtct
     3541 acgatcaatt cggcctgaaa gccttccatg ccggttcgac cggcgtgcag atgggcggct
     3601 ggttcaacaa ggagatcacc ggcgtcgact ccatcaaagg tatgaagatg cgcattccgg
     3661 gcatcggcgg cgaagcgctg gccaagctcg gcatgacgat catcaacc
//
LOCUS       DASUMG010000470         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_143621, whole genome shotgun sequence.
ACCESSION   DASUMG010000470 DASUMG010000000
VERSION     DASUMG010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143621"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1882
                     /locus_tag="VFX46_07755"
     CDS             275..1882
                     /locus_tag="VFX46_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX5600515.1"
                     /translation="MPKIVSQINTSTRAFRANAEAMRALVADLEQKRAEAAKGGPEKL
                     RQRHVARGKLLPRDRVMRLLDPGTPFLELSPLAAYGMYSGDIHGAGIITGIGRVAGRE
                     CMIVANDATIKGGTYFPMTVKKHVRAQEIARENRLPCIYLVDSGGANLPHHTEVFPDK
                     EHFGRIFYNQARMSAEGIPQIAVVMGSCTAGGAYVPAMSDESIIVRRQGTIFLGGPPL
                     VKAATGETVSAEDLGGADVHTRLSGVADHYALNDAHALEIARSIVANLNTRKQIDVAL
                     AEPRPPAYPIEELDGIVPASLATQYDSREVIARLVDGSEFDEFKERYGTTIVTGFAHI
                     EGMPVAILANNGILYSESAQKAAHFIELACQRRIPLLFLQNITGFMVGRDAEAGGIAR
                     HGAKMVTAVACANVPKITVIIGGSYGAGNYAMCGRAYGPRFLFMWPNARISVMGGQQA
                     ASVLATVRRDNLEAEGKTWTAEEEAEFKRPILEQFEREGHPYYATARLWDDGIITPQE
                     TRRVLALALSACLNAPIPETRFGVFRM"
     gene            complement(1950..>2259)
                     /locus_tag="VFX46_07760"
     CDS             complement(1950..>2259)
                     /locus_tag="VFX46_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX5600516.1"
                     /translation="LDTHPPLLEHVAERMRMTPRTLQSRLAQHGTTVEKVLSDTRRAL
                     AERYLRDTQLQLTEIAYLLGFSEQSAFTRAARSWFGRPPRQLRKESFQPARNGNGHSG
                     "
BASE COUNT          411 a          694 c          746 g          408 t
ORIGIN      
        1 gtgtcgccgt ggcgctggct gagggcaagg atctggccgc catcgcggag gagcaccgag
       61 tttccatcgg gaccattcga agccagctca agtcgctgat ggcgaagacc gacacgcatc
      121 gccaggccga gctggtcacg cttgttctcc ggtacaccgt gaaccaggtc cgttaaacgt
      181 cgaaattgcg ggttgcgggt gccgcctggg cgatctcact tgcggactcc cgctcgcgct
      241 gcttgatatg gcgccgcgtc ctccgagagg tgccatgccg aagatcgttt cccagatcaa
      301 cacgtcgacc cgagcttttc gcgctaatgc cgaagcgatg cgagcgctcg tcgccgatct
      361 cgaacagaag cgcgccgagg cggctaaggg cggtccggag aagctgcggc agcgccacgt
      421 tgcccgcggc aagctgttgc ctcgggatcg cgtgatgcgg ctgctggacc ccggcacgcc
      481 gttcctcgag ctgtcgccgc tcgccgccta cggcatgtat tcgggcgaca ttcacggtgc
      541 cggtatcatt accggcattg gccgcgtggc aggtcgggag tgcatgattg tcgcgaacga
      601 cgccacgatc aaaggcggca cctacttccc gatgacggtc aagaagcacg tccgcgccca
      661 ggagattgcg cgggagaacc ggttgccgtg catctatctc gtcgacagcg gcggggccaa
      721 ccttccgcac cacacggagg tctttccaga caaggagcac ttcggccgca ttttctacaa
      781 tcaggcgcga atgtcggcgg agggcatccc gcagattgcg gtggtgatgg gcagctgcac
      841 cgcaggcggc gcctacgtac cggccatgtc cgacgagagc atcatcgtgc gccgccaggg
      901 gacgatcttc ctcggcgggc ctccactggt gaaggcggcg acgggcgaaa cggtgagtgc
      961 tgaggacctc ggcggcgcgg atgtgcacac gcggctttcc ggcgtggcgg atcattatgc
     1021 gctcaacgat gctcacgcgc tcgagatcgc gcgcagcatc gtcgccaacc taaatacgag
     1081 gaagcagatc gacgtcgctc tcgcggagcc gcggccgccc gcctatccga tcgaagagct
     1141 ggacggcatc gtgccggcgt cactcgcaac ccagtacgac tcgcgcgagg taatcgcgcg
     1201 gctcgtcgac ggatcggagt tcgacgagtt caaggagcgt tacgggacaa cgatcgtcac
     1261 cggcttcgcg cacatcgagg gaatgccggt ggccatcctc gcgaacaacg gcattcttta
     1321 ttccgagtcc gcacagaagg ccgcgcactt catcgagctg gcctgtcagc ggcgcattcc
     1381 gctgctgttc ctgcaaaaca tcaccgggtt catggtcggc cgggacgcgg aggcaggcgg
     1441 cattgcgcgc cacggcgcca agatggtgac agcggttgct tgcgcgaacg tgcccaagat
     1501 caccgtcatc atcggcggca gctatggtgc cggcaattac gccatgtgcg gacgcgccta
     1561 cgggccacgc ttcctcttca tgtggccgaa tgcgcgcatc tccgttatgg gcggtcagca
     1621 ggccgcgtca gtgctggcga cggtgcggcg ggacaacctc gaagccgaag gcaagacgtg
     1681 gacggctgag gaagaggcgg aattcaagag accaattctg gagcagttcg agcgcgaggg
     1741 gcatccgtat tacgccacgg cgcgtctttg ggacgatggc atcatcacgc ctcaggaaac
     1801 acggcgtgtc ttggcgctcg cgctgtcggc ctgcctgaat gcaccgatac cggagacccg
     1861 gttcggagtg ttccgcatgt agagcggagc agctgtttct tttccgatgg tgaaataatc
     1921 gccactctcc actgcgcggt gggcgtaaat cagccgctat gcccgtttcc attccgcgca
     1981 ggctggaaac tttccttgcg caattgacgg ggtggtcggc caaaccagct ccgtgcggcc
     2041 cgggtgaacg cgctctgctc ggagaagccg agcagatatg caatctctgt cagctgcagc
     2101 tgcgtatcgc ggagatagcg ctcagccagg gcgcggcggg tatcgctcag gactttctcg
     2161 acggttgtgc cgtgctgggc taaacgactc tgaagcgtgc ggggcgtcat ccgcattcgt
     2221 tcggccacgt gctcgagcag gggaggatgc gtatcgagt
//
LOCUS       DASUMG010000471         2682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_464028, whole genome shotgun sequence.
ACCESSION   DASUMG010000471 DASUMG010000000
VERSION     DASUMG010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464028"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFX46_07765"
     CDS             complement(<1..452)
                     /locus_tag="VFX46_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX5600517.1"
                     /translation="MQDGSVRRRFFAQAAVLVAVLVTAFGLLSAMPASAQDEWGSSYI
                     TPFPQDDVYRLQVIGDGMAEGLFSALSETFRSDTRVRVPNRLHSLNGLIRLRPDDFFK
                     FEETIKSEPLHIAVIMLGISDRVALRAPDGRRVNVGTEEWKEEYGRRI"
     gene            complement(460..1707)
                     /locus_tag="VFX46_07770"
     CDS             complement(460..1707)
                     /locus_tag="VFX46_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5600518.1"
                     /translation="MSRIISKAFAALVLVMALMAGSATGETSESQIAPLVELQRFLQQ
                     VRAEALKAGVSAATFDRATRGLEPDTQVLVLAASQPEFVKPVWEYLAGLVSDARIAAG
                     QQKLAEHADTLAAIERTYGVDRHVVLAIWGVESNYGTAQGERSVIRSLATLAAGDERR
                     SRFWRREFIAALRILEQGDVAPERMTGSWAGAMGHTQFMPTTYHERAVDFDKDGRRDI
                     WDSVPDGLASTANYLKAFGWRMEDPWGFEVILPNGFDYRLSAPGNEKPASFWRQVGVE
                     RPGGVAVRDTSSPLQLILPAGANGPAFLVTRNFRAILRYNPAEAYALAVGHLADRIAG
                     AEGIASAWPTHDRPLARAEREELQKLLSERGLDTGPPDGIIGRQSRAAIRAFQRTSGL
                     PEDGYPSLQLLNLLRETGGSGRE"
     gene            complement(1718..>2682)
                     /locus_tag="VFX46_07775"
     CDS             complement(1718..>2682)
                     /locus_tag="VFX46_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX5600519.1"
                     /translation="AQLARLAAAGRALQAEHAETLREIERRFGVEGHAVLAIWGRETA
                     FGAHQLPHYAFRALATQAYLGRRKEMFRNELLHALKLVDEGSATFEQMRSSWAGAIGL
                     PQVMPSEFHRFAYDLDGDGRKDIWTSIPDALATIARQLQAKGWVKGQTWGYEVRLPPS
                     VDCSLEGPANARPLSEWIAMGVTRTFKRQFPEHVHSAEAYLMAPGGAYGPAFLVLENY
                     RVIRRYNMSDLYAVFVGNLADRIAGGGDFETPWQNITQLSTREVEEIQRLLKELGYAI
                     GEVDGKVGSITRWQIGKFQRDNRLKVDCWPTKELLRRLRSAAAP"
BASE COUNT          521 a          894 c          783 g          484 t
ORIGIN      
        1 tcgattctcc gtccgtactc ctccttccac tcttccgtac cgacgttgac acgcctgcca
       61 tccggcgcgc gcaaagcgac ccgatccgaa atgccgagca tgatcaccgc gatgtgcagc
      121 ggttcactct tgatggtctc ctcgaacttg aagaagtcat cgggacgcaa tcggatcagc
      181 ccattgaggc tatgcaatcg attgggcacc cgcacgcgcg tatccgaacg aaaggtctcg
      241 gagagggcgc tgaacaagcc ttcggccata ccatcgccga tgacctgcag ccgataaacg
      301 tcatcctgcg ggaaaggcgt gatgtagctc gatccccact cgtcttgggc tgacgctggc
      361 atcgccgaca ggaggccgaa agcggtgacc aatacggcaa ccaacacggc cgcctgcgcg
      421 aaaaaccgac gtctgaccga gccgtcttgc acctcagtac tactcccgtc cagatccgcc
      481 ggtctctcga agcagattga gcaactgcag gcttggatat ccatcttccg gcagcccgct
      541 ggttcgctgg aatgcacgaa ttgcggcacg ggactgcctg ccgatgatcc cgtccggagg
      601 accggtgtca agcccgcgtt cggataagag tttctgcaat tcttctcgct cggcacgggc
      661 aagcgggcga tcgtgcgtcg gccaggctga cgcaatcccc tcagcgccgg cgattcgatc
      721 tgccagatga ccgactgcca gggcataagc ctccgctgga ttatacctca gaatggcccg
      781 gaagttccgt gtgacgagga aagccgggcc gttcgctcct gccggtaaaa taagttgtag
      841 aggcgaactc gtatcgcgga ctgcaactcc tcccgggcgc tcgactccaa cttgtcgcca
      901 gaacgaggct ggtttctcgt tccccggcgc cgacaatcga tagtcaaacc cgttcggcaa
      961 gatcacctcg aagccccaag ggtcctccat tctccagccg aaggccttga ggtaattcgc
     1021 tgtcgaggca aggccgtccg gaacgctgtc ccagatatcg cgccgtccgt ctttgtcgaa
     1081 gtcaacggcg cgttcgtggt acgtcgtggg cataaactgc gtgtgcccca tcgcccccgc
     1141 ccacgagccg gtcatgcgct ccggcgcgac gtccccctgc tccaggatgc gcagcgccgc
     1201 aatgaactcg cgccgccaga agcgggatcg ccgctcgtcc ccggcagcaa gcgtggcgag
     1261 cgaccggatg acactgcgct cgccctgcgc cgtgccgtag ttcgactcga caccccaaat
     1321 cgccagcacc acgtgccggt ccacgccgta agtccgctcg atggcggcca aagtgtcggc
     1381 gtgctcagcc agcttctgct gccctgcagc gatccgggcg tcgctgacga ggccggcgag
     1441 gtactcccat acgggcttga cgaactccgg ctgcgaagcc gccaagacca acacctgggt
     1501 atcaggctca aggccgcgcg ttgcgcgatc gaaggtcgct gcgctcactc cagccttcag
     1561 agcctcggcc ctcacctgct gcagaaatcg ctgcagctcg accagcggag caatttgcga
     1621 ctcagatgtt tcgcctgtcg cagaaccagc catgagcgcc atcacaagta ccagggctgc
     1681 aaaggccttc gatataatcc tagacaccgt tcccctccta tggcgccgca gcagaccgga
     1741 gccgccgcag cagctccttt gtcggccagc agtccacctt caggcggttg tctctttgaa
     1801 attttccgat ctgccagcgc gtgatggaac ccactttccc gtccacctcg ccgatggcgt
     1861 agcccaattc cttcaataga cgctgaatct cctccacctc acgggtggaa agctgcgtga
     1921 tattctgcca cggcgtctcg aagtcaccgc cgccggcgat ccgatccgca aggttgccga
     1981 cgaagacggc gtagagatcc gacatgttgt accgccggat gacgcggtaa ttctcgagga
     2041 cgagaaacgc cggcccataa gcgccgccgg gtgccatcag gtaggcctcg gcagaatgga
     2101 cgtgctccgg gaactgtcgc ttgaaagttc gcgtgacgcc catagcgatc cactcggaga
     2161 gcggccgagc gttcgcaggc ccttccaacg agcaatcgac agaaggcggc agccggacct
     2221 cgtagcccca ggtctggccc ttcacccagc ctttggcctg cagctgccgt gcgatggtgg
     2281 cgagcgcatc aggaattgag gtccagatgt ccttgcggcc gtcgccgtcc aggtcgtagg
     2341 caaacctgtg gaactcagac ggcatcacct gcggcagacc gatcgcgccg gcccaggatg
     2401 atcgcatttg ctcaaaggtc gcggagccct cgtcgacgag cttcaacgca tgcaacagct
     2461 cgttccggaa catctctttg cggcgaccga ggtaagcctg tgtcgcgaga gcgcgaaatg
     2521 catagtgcgg cagttggtgt gcgccgaaag ccgtctcgcg gccccagatt gccagcacgg
     2581 catggccttc gacaccgaac ctgcgctcga tttcgcgaag cgtttcggcg tgctcggctt
     2641 ggagagcgcg cccggcagca gcaaggcggg cgagctgagc gc
//
LOCUS       DASUMG010000472         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_545867, whole genome shotgun sequence.
ACCESSION   DASUMG010000472 DASUMG010000000
VERSION     DASUMG010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545867"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /gene="selA"
                     /locus_tag="VFX46_07780"
     CDS             <1..807
                     /gene="selA"
                     /locus_tag="VFX46_07780"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX5600520.1"
                     /translation="QAISQNTGLILKAHTSNYLIQGFTKSVPATELSALARRHQIPFI
                     HDLGSGALVDLGRYGLRPEPTVRECLAEGADLVTFSGDKLLGGPQAGIVAGRKDLVAL
                     VRKNPMKRALRIDKIRLAALEAVLKLYRDPDRLHQTLPTIRYFARSQQDIEAAAARLQ
                     GPIAAAVGPAYVVSITQCSSQIGSGALPLETMPSAGIAIAPAERKGAGTRLEALATAF
                     RKLPVPVIGRINKGEFLLDLRCLDDEATFLGQLDQLRSVLGAEEPSHAKG"
     gene            952..>2777
                     /gene="selB"
                     /locus_tag="VFX46_07785"
     CDS             952..>2777
                     /gene="selB"
                     /locus_tag="VFX46_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX5600521.1"
                     /translation="MIVGTAGHIDHGKSSLVMRLTGIDPSRLKEEKVRGITIDLGFAY
                     WPRPNGEIIGFVDVPGHERLVHNMLAGATGIDYVMLVVAADDGVMPQTREHLAIVDLL
                     GLEHGVVALNKCDLVTPERLSAVTTDIENLLLGTTLEGSEIIPVSAATGEGVDLLAAH
                     LDEALGRVRARPKTGRFRLAVDRSFTLAGLGTAVTGTVLSGSVQINDRVIVSPAGLEA
                     RVRSIHAQNQPVGRGEAGQRCALVLSGPQISKESVSRGDVVLDPTLHAPTTRIDATLR
                     VLPSEPRPITQWFPVKVHHAAAEVPGRVVLLRDEPIAPGESALVQLVLERPIAAAAGD
                     RFIIRDTTSSRTIGGGALIDLRAPERRRRTPERRATLAGLAEQDPVTALQRVLSVPGS
                     WIDLDAFFRDRAMGEEAAAAVTSELGLITLPYGNSRAAMLPHSWDRFRQRIGELLDAF
                     HEKNPEKPGIGLEQLRQAADKPPLPVPIFTAALRKLIEAGEVALDRTWVRRPGHEVKF
                     SPEEEQIWQGIKPMLARERFRPPRVRDIASAMGIPEAFVRRLMHMAARRGDVDEIAHD
                     HFFLRSTVQEMAQIAIDIAEKSPSGKFTAAEFRDRLQNGRKVA"
BASE COUNT          520 a          867 c          881 g          509 t
ORIGIN      
        1 caggcgatca gtcaaaacac ggggttgatc ctcaaggccc acacatcgaa ctacctgatc
       61 cagggattta cgaaatccgt tcccgccacc gagctttcgg cgctggcgcg gcggcaccag
      121 atccccttca tccatgatct gggttctggc gctcttgtcg atctcggccg atatggcctt
      181 cggccagagc caaccgtgag ggaatgcctt gccgaaggcg cggatctcgt caccttctcg
      241 ggagataagc tgctcggtgg cccacaggcg gggatcgtcg ccggccgaaa ggatctggtt
      301 gcgctcgtcc gcaagaaccc gatgaagcgc gcgctacgaa tcgacaagat tcgcctggcg
      361 gcgctggaag ccgtgctcaa gctttatcgc gatcccgacc ggcttcacca aacgttgccg
      421 acgatcaggt acttcgcccg ctcgcagcag gacatcgagg ctgcggcggc gcggctgcag
      481 ggcccaatcg cggcggccgt cggcccggct tacgtcgtca gcatcaccca atgcagcagt
      541 cagattggct ccggcgcttt accgctcgaa actatgccga gcgccggcat cgcaatcgct
      601 ccagccgagc ggaagggcgc gggcacacgc ctcgaagctc tggcgacggc attccgcaaa
      661 ctgcccgtcc cggtgatcgg tcgcatcaac aaaggcgagt ttctgctcga tcttagatgc
      721 ttggatgatg aggccacgtt ccttggccag ctcgatcagc tgaggagcgt tctcggcgca
      781 gaagagccgt cccatgcaaa aggctgacgg tgtgtgtaga gatcaaatcg aagtgaattg
      841 gtgtgaggac ccgagagttg taggtcgggg ctattaccgc gcgacaatta ttgacgtatt
      901 ggatttggtg atcggtaagc agcggcgacc accgggttcg gaggttgctc aatgatcgtt
      961 ggcaccgccg gacacatcga tcatggcaag tcatccctcg tgatgcggct gacgggcatc
     1021 gatcccagcc gcctgaagga ggaaaaggtc cgcggcatca cgatcgacct gggatttgcc
     1081 tattggccgc ggccaaacgg cgaaatcatc ggcttcgtgg atgtgcctgg tcacgagcgc
     1141 ctcgtgcaca acatgctcgc gggcgcaact ggcatcgatt acgtgatgct cgtcgtcgcc
     1201 gccgacgatg gcgtgatgcc ccaaacccgc gagcatttgg ccatcgtgga tctcctgggg
     1261 cttgagcacg gcgttgtcgc gctgaacaag tgcgatctcg tcacgcccga aaggctgtct
     1321 gcagtcacca cggacatcga gaacctcctg ctgggcacca ccctcgaagg gtccgaaatc
     1381 atccccgttt cagccgcaac cggtgagggg gtcgatctcc tggcagcaca tctcgatgag
     1441 gcgcttggac gtgtcagagc acgcccgaag acaggacgtt tccgccttgc cgtcgatcgt
     1501 tccttcactc tggccggcct cggcacagcc gtcaccggga cggtgctgtc aggcagtgta
     1561 cagatcaacg atcgcgtgat tgtcagccca gcggggctgg aggctcgtgt acgttctatc
     1621 cacgcccaga accagccggt ggggcggggg gaagcggggc aaaggtgtgc gcttgttctg
     1681 agcgggccac agatcagcaa ggagtcggtg agccgcggag acgtggttct cgatcccact
     1741 ttgcatgcgc cgacgacgcg gatcgacgcc acgctccgtg tcctgccatc ggagcccagg
     1801 cccattacgc agtggttccc ggtcaaggtg caccacgcgg cggctgaagt acctggccgc
     1861 gtggttctct tgcgcgatga gccgattgcg cctggtgagt cggcactcgt gcagctcgtt
     1921 ctcgagcgcc cgatcgcagc tgcggcgggt gatcggttca tcatccgcga cacgacgtcg
     1981 agccgaacca tcggcggtgg tgcgctcatc gatctgcgcg cacccgagcg gcgccgtcga
     2041 acaccggaac ggcgcgcaac tctcgccggc ctcgccgagc aggacccggt gacggcgctc
     2101 cagcgcgtcc tgtcggtgcc tggaagctgg atcgatctcg atgccttctt ccgggatcgg
     2161 gcgatgggtg aagaggcggc cgcggccgtg acatcagagc tggggctcat cacgctgccg
     2221 tatggaaaca gccgggccgc aatgctgccc cacagctggg accgtttccg ccagcgtatt
     2281 ggcgagctgc tcgatgcttt tcacgagaag aaccccgaaa aacctggcat tggtctagag
     2341 cagttgcggc aggccgctga caagccaccg ctcccggttc caatcttcac tgccgcgctg
     2401 cgaaagctga tcgaggcggg ggaggtcgca ctcgatcgca cctgggtccg ccgccctggc
     2461 cacgaggtca agttctctcc ggaagaggag cagatctggc aaggcatcaa gccgatgctt
     2521 gcacgtgagc gctttcgccc gccgcgcgtt cgcgacatcg ccagtgccat gggcatccct
     2581 gaagcatttg tccgccggct gatgcatatg gcggcgcgcc gcggcgacgt cgacgaaatc
     2641 gcgcacgatc acttcttcct gcgatcgacc gtgcaggaga tggcgcaaat cgcgatcgac
     2701 attgcggaaa aatctcccag cggcaaattc acggcagcgg aattccgcga tcgcttgcaa
     2761 aacggccgta aggtggc
//
LOCUS       DASUMG010000473         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_866193, whole genome shotgun sequence.
ACCESSION   DASUMG010000473 DASUMG010000000
VERSION     DASUMG010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866193"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..827
                     /locus_tag="VFX46_07790"
     CDS             <1..827
                     /locus_tag="VFX46_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600522.1"
                     /translation="RNMSMIFQSYALWPHMTVAENIVYGLKLRKMDRETIGKKLNAIL
                     ATTKLAPLAERYPGELSGGQQQRVALARALIVEPETLLLDEPLSNLDANLREEMRFEV
                     RRLHDAYRYTTVYVTHDQSEAMTTADLICVMNGGKIEQSGPPEEIYDRPSSEFVARFI
                     GSSNVIRGKGLDGSSVDLAGTPLKCSGAKISLGMATPVSIRPHDIAISATQRQGENVV
                     PATVVRQVFLGGSRDYMVEAKDGTQLRVLTGAGENIPQGSAVWLYLPPERCRALRG"
     gene            873..1940
                     /locus_tag="VFX46_07795"
     CDS             873..1940
                     /locus_tag="VFX46_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX5600523.1"
                     /translation="MKRSKISRRDVLVGSSALVAGAAFSTRVMSAAPPAAAVTPALIE
                     AAKKEGKVVWYTSVDLKLSEQIGKAFEAKYPGVTCKVERTGAERLLQRIGQEYTANVH
                     AVDVVNSSDASHLVYWKDQGMLAPYVPEDVAKFYPAEHKDVDGNFASFRVFLCVIAYN
                     TNLVKKEEAPKSFADLLDPKWKGKIIKAHPGYSGTIMTATYQVSRDLGWSYFEKLAQQ
                     NILQVQSAADPPKKLALGERAIQADGVEYLIFQEKEKGQPVEPVYATEGTPIIIGPNG
                     LFKAAPNPNAARLFQSFCFSPECQKLAVEIGGLRSVHPQVPEHQGRTPLSKIKLMKDD
                     AAAVLKNGEQIKARYVKIFHV"
     gene            1995..>2434
                     /locus_tag="VFX46_07800"
     CDS             1995..>2434
                     /locus_tag="VFX46_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX5600524.1"
                     /translation="MTTVASTAPIARRRLGFDLSKPVLLAFAAILFALIAMPLSWLIF
                     YAFTDKSGAFTLDNFHRLVSDAAYLDPLLTTFALATLSALICCVVAAPMGWLVARTDM
                     PLRRTVRLLVTASFVTPPFLGAIAWELLAAPNSGLLNQLYRELTG"
BASE COUNT          483 a          734 c          770 g          447 t
ORIGIN      
        1 ggcgcaacat gtcgatgatc ttccagagtt acgcgctgtg gccgcacatg acggtcgccg
       61 agaacattgt gtatgggctc aagctcagga aaatggaccg ggagacgatc ggcaagaagc
      121 tcaatgcgat ccttgcgacg accaagctgg cgccgctggc ggagcgctat ccgggcgaat
      181 tgtcgggcgg acagcagcag cgcgtcgcgc tcgcacgcgc gctgattgtc gagccggaga
      241 cgttgctgct cgacgagccg ctctccaatc tcgacgcgaa cctgcgcgag gagatgcggt
      301 tcgaggtgcg acggctgcat gacgcgtacc gctacacgac ggtctacgtc acccacgacc
      361 agtcggaggc gatgaccacc gccgacctaa tttgcgtcat gaacggcggc aagatcgagc
      421 agtccggtcc gccggaggag atctacgacc ggccgagttc ggagttcgtc gcgcgcttca
      481 ttggctcgag caacgtcatc aggggtaaag gtctcgacgg atcaagcgtc gatcttgccg
      541 gcacgccgct caagtgcagc ggggcgaaaa tcagcttggg catggcgacg ccggtatcga
      601 tccgcccgca cgacatcgcg atttccgcaa cgcagcgcca gggcgaaaac gtcgtgccgg
      661 cgaccgtggt tcggcaggtc tttctcggcg gcagccggga ttatatggtt gaggcgaagg
      721 atggcaccca gctgcgcgtg ctcaccggtg cgggcgagaa catcccgcaa ggcagtgccg
      781 tctggctgta cctgccgccg gagcgctgcc gcgcactgag gggataataa gtcgggggca
      841 gacgactaat cagggcaatg gaggatacgt caatgaaacg ctcaaaaatt tcacgccgcg
      901 acgtgctggt cggctcgagc gcgctcgttg ccggcgccgc attttcgaca agggtcatgt
      961 cggcggcacc gcccgcggcg gccgtcacgc cggcgttgat cgaagccgcc aagaaagaag
     1021 gaaaggtggt ctggtacacc tcggtcgacc tcaagctctc cgagcagatc ggcaaggcct
     1081 tcgaagcgaa atatcccggt gtgacgtgca aagtggagcg caccggcgcg gaacggctgc
     1141 tgcagcgcat cggccaggaa tacacggcga atgtgcacgc ggtcgacgtg gtcaactcgt
     1201 cggacgcgtc gcacctcgtc tactggaagg atcagggcat gttggcgccc tacgttccgg
     1261 aagacgtcgc gaaattctat ccggccgagc acaaggatgt tgatggcaac ttcgccagct
     1321 tccgcgtgtt tctgtgcgtc attgcctaca acaccaacct ggtgaagaaa gaagaggcac
     1381 cgaaaagctt cgccgatctg ctcgacccga agtggaaggg caagatcatc aaggcgcatc
     1441 cgggttacag cggcaccatc atgaccgcga cctaccaggt ctcgcgcgat ctcggctgga
     1501 gctacttcga gaagctcgcg cagcaaaaca tcctgcaggt gcagtcggct gccgacccgc
     1561 cgaagaagct cgcgcttggc gagcgtgcta tccaggccga cggcgttgaa tacctgatct
     1621 tccaggaaaa ggagaagggc cagcctgtcg agccggtcta tgccaccgag ggcacgccaa
     1681 tcatcatcgg gccgaacggg ctgttcaagg cggcgccaaa tccgaacgcg gcgcggctgt
     1741 tccagagctt ctgcttctca ccggagtgcc agaagctcgc ggtcgaaatt ggcggcttgc
     1801 gttccgtgca tccgcaagtt ccggaacatc aaggccgtac gccgctgagc aagatcaagc
     1861 tgatgaaaga tgatgcagcg gccgtgctta aaaacggcga acagattaag gcgcgttacg
     1921 tcaagatctt tcacgtgtaa cggttgagcc tccccacaca ggggaggcta ttctttgctc
     1981 gcacggtctg ttcgatgaca acggtcgcat caaccgcacc gattgcaagg cggcggctcg
     2041 gcttcgatct gtcgaagccg gtgctgctcg ccttcgcggc gattctattc gcgctgatcg
     2101 cgatgccgct ctcatggctg atcttctacg ccttcaccga caagagcggt gccttcaccc
     2161 tcgacaattt ccatcggctg gtgtccgacg cggcctatct cgatccgcta ttgaccactt
     2221 ttgcgcttgc caccctgtcg gcgttgatct gctgcgtggt ggcggcgccc atgggctggc
     2281 tggtggcgcg caccgatatg ccgctgcgtc gcacggttcg cctgctggtg accgcctcgt
     2341 tcgtgacccc gccattcttg ggagcgattg cttgggaact gttggcggct ccgaacagcg
     2401 ggctgctcaa tcagctttac cgcgagctta ccgg
//
LOCUS       DASUMG010000474         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_305212, whole genome shotgun sequence.
ACCESSION   DASUMG010000474 DASUMG010000000
VERSION     DASUMG010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305212"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VFX46_07805"
     CDS             complement(<1..414)
                     /locus_tag="VFX46_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600525.1"
                     /translation="MPEVASHVVAHAELTSSDLAELRRLFDAEYQNDFGEWDPDMPYS
                     YAPHAVHVIARSNGEAVGHVGWARRTVGVGGDETEIAGVGGVLISDALRGERVASRLM
                     GSAAESLKAAGGIAFGYLGCREEVVSFYASCGWTRI"
     gene            complement(418..738)
                     /locus_tag="VFX46_07810"
     CDS             complement(418..738)
                     /locus_tag="VFX46_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX5600526.1"
                     /translation="MSNSSTTTDADLIEPIHPGEILMEDYIEGFEITQNKLAVSIGVS
                     PRRINEIVHGKRGITADTAIRLARYFGTSEEFWMNLQTNYELRLERRALRDKVAAITP
                     LKVA"
     gene            complement(713..994)
                     /locus_tag="VFX46_07815"
     CDS             complement(713..994)
                     /locus_tag="VFX46_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX5600527.1"
                     /translation="MIRSFGSKDTERIWHEQYVKGVDRTVQRATLRKLELVHAAKDVE
                     DLRIPPGNRLERLAGDRRGQHSIRVNAQWRICFVWRDGGAEDVELVDYH"
     gene            complement(1028..2113)
                     /gene="ychF"
                     /locus_tag="VFX46_07820"
     CDS             complement(1028..2113)
                     /gene="ychF"
                     /locus_tag="VFX46_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX5600528.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTRAQVLAANYPFATIEPNVGVV
                     PLPDPRLKTLAGIFGSERILPATVSFVDIAGIVKGASEGEGLGNKFLANIREADAICQ
                     VTRAFADPDVVHVSGAVDPEGDIETIATELILADLQTIENQLPRLEKEVKAKKADASL
                     LAAIKEAQKVLETGTTLFAGAAAAKIDLAEIAPLQLMTTKPFIYVFNMDEAGLADEAL
                     KARLSGLVAPAEAIFLDAQFESELAELTEDEAAEMLEDAGYDEAGLDKLARVGFQTLG
                     LQTYLTAGPKESRAWTIHKGDTAPQAAGVIHTDFQRGFIKAEVIGFDDLVAAGSVAAA
                     RAAGKARIEGKDYVMRDGDVVEFRFNV"
     gene            2168..>2621
                     /gene="rmuC"
                     /locus_tag="VFX46_07825"
     CDS             2168..>2621
                     /gene="rmuC"
                     /locus_tag="VFX46_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX5600529.1"
                     /translation="MDAFGWLVSGLTLLLGLVAGAAGLWWLVLRARYLELAAEHGEAV
                     ERLAGLQASLASEQARNEFLQAHAAGERERAGQDQSVLQVLAPVTEKLRQVQQQVTVL
                     ERDRVEQFGQLSQQLITAAHQDAAILRTTASLAGALRNTSARGTWGEVQ"
BASE COUNT          452 a          892 c          807 g          470 t
ORIGIN      
        1 gatccgagtc cagccacacg aggcgtagaa agagaccacc tcctcgcgac aaccgagata
       61 gccgaaggcg atgccgccgg cggccttcaa cgactccgcc gcactgccca tgagtcgact
      121 cgcgacgcgc tcacctctca gagcatccga gatcaataca cccccaaccc cggcaatctc
      181 agtttcatcg ccaccgacac ccaccgtccg ccgcgcccag ccgacatgac cgaccgcttc
      241 gccgttactc cgagcgatga catgaacagc gtgaggtgca tacgagtacg gcatgtcagg
      301 gtcccactca ccgaagtcgt tctggtactc ggcgtcgaag agccgccgaa gttcagcgag
      361 atcactggag gtcagttcgg cgtgcgcaac gacatggctc gcaacttctg gcatgcgtca
      421 tgcgaccttc agcggcgtga tcgcagcaac cttgtcgcgc agcgcacgac gttccagccg
      481 cagctcatag ttcgtctgca ggttcatcca gaactcctcg gacgtcccga agtaccgcgc
      541 cagacggatc gctgtatccg cagtgatccc ccgcttgccg tggacgatct cgttgatcct
      601 acgcggcgac acaccgatcg ataccgcgag cttgttctgc gtgatctcga agccttcgat
      661 gtagtcctcc atcaggatct cccccggatg gatcggctcg atcaggtcag catcagtggt
      721 agtcgacgag ttcgacatct tccgcacctc catctctcca gacaaagcag atccgccact
      781 gggcattcac ccggatgctg tgctgacccc gacgatctcc ggccaaccgc tccaaccggt
      841 tccccggcgg gatccgcaga tcctcgacat ccttcgccgc gtgaaccaac tcgagtttgc
      901 gcaacgtcgc ccgctgcacg gttcgatcaa cgcctttgac gtactgctcg tgccagattc
      961 gctcggtgtc cttgctcccg aatgatctga tcacccgacc atcatataac ggagagcgtc
     1021 attgacgcta tacgttaaat cggaattcga ccacgtcgcc gtcgcgcatg acgtagtcct
     1081 tgccctcaat gcgggccttg cccgccgccc gggcggcagc aaccgagccg gcggcgacga
     1141 ggtcgtcgaa gccaatgacc tcggccttga tgaaaccgcg ctggaaatcc gtgtggatga
     1201 ccccggcggc ctgcggcgcc gtgtcgccct tgtggatcgt ccaggcccgg gactccttcg
     1261 gaccggccgt gagataggtc tgcaggccca gggtctggaa gccgacgcgg gccagcttgt
     1321 ccagcccggc ctcatcgtag ccggcgtcct cgagcatttc cgccgcctcg tcctcggtca
     1381 gctccgcgag ctcggattcg aattgcgcat ccaggaagat ggcctcggcc ggcgccacga
     1441 gaccggagag ccgggccttc agcgcctcgt cggccagccc cgcctcgtcc atgttgaaca
     1501 cgtagatgaa tggcttggtc gtcatcagct gcagcggggc gatctcggcc aggtcgatct
     1561 tcgccgcggc ggcgccggcg aacagcgtgg tgcctgtttc gagcaccttc tgcgcttcct
     1621 tgatggccgc cagcagcgag gcgtcggcct tcttggcctt gacttccttc tccagccgcg
     1681 gcagctggtt ctcgatcgtc tgcaggtcgg cgaggatcag ctcggtggcg atcgtctcga
     1741 tgtcgccctc ggggtcgacg gcgccgctga cgtgcacgac gtcgggatcg gcaaatgcgc
     1801 gggtgacctg gcagatcgcg tcggcctcgc ggatgttggc gaggaacttg ttccccagcc
     1861 cctccccctc ggacgcgccc ttgacgatgc ccgcgatgtc gacgaaggag accgttgccg
     1921 gcaggatgcg ctcggagccg aagatcccgg cgagcgtctt cagccgggga tccggcagcg
     1981 gcacaacccc cacattgggt tcgatcgttg cgaacgggta gttggccgcg aggacctgcg
     2041 cccgggtcag tgcgttgaac agggttgact tgccgacatt gggcagtccg acgattccaa
     2101 tagtaagagc cacgggagtc cactctacct tcccccaact gtcggtgctg gctggcaaag
     2161 taaaggcatg gatgcattcg gatggcttgt gtcaggcctc acgctgttgc tgggtctcgt
     2221 tgccggggcc gccggcttgt ggtggctcgt gctgcgcgca cgctacctcg agctcgccgc
     2281 cgagcacggc gaggcggttg agcggcttgc ggggctgcag gcgtcgctgg cctcggagca
     2341 ggcccgcaac gagttcctgc aggcgcacgc cgccggggaa cgcgaacgcg ccggccagga
     2401 ccaatccgtc ctgcaggtgc tcgcgccggt aacggaaaag ctgcggcagg tccagcagca
     2461 ggtgacggtg ctcgaacgcg accgggtgga gcagttcggc cagctcagcc agcagctcat
     2521 cacggcggct caccaggatg ccgccatcct gcgcacgacg gcgtcgctcg ccggagcgct
     2581 gcgcaacacc tccgcacggg gaacctgggg cgaggtccaa t
//
LOCUS       DASUMG010000475         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_625484, whole genome shotgun sequence.
ACCESSION   DASUMG010000475 DASUMG010000000
VERSION     DASUMG010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625484"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VFX46_07830"
     CDS             complement(<1..433)
                     /locus_tag="VFX46_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX5600530.1"
                     /translation="MRAAVCKSLIGPEGIVIEDLPVPEPGPGEVRVRVKAVGINFLDT
                     LMTRGKYQHKPELPFSPGAEIAGEIETLGEGVEGFSPGQRVCAYLGWGGAREFVIVRE
                     EQLSPVPEGVSYEVAAGINVTYGTAIHGLKERGRLQAGETVA"
     gene            530..1069
                     /locus_tag="VFX46_07835"
     CDS             530..1069
                     /locus_tag="VFX46_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5600531.1"
                     /translation="MNERTPNWLRENWPPKPRGYFAIGAERISKALNLGNLMRSAHAF
                     GASFTFTIGATYQALEARADTSKGQWHLPHYNWAKVEDLVLPEGCKLVGIELTEDAID
                     LPSFYHPLRAAYVLGPERGSLSEALLARCDHVVRIPTAFCINVAMAGAIVMYDRVKTL
                     ARFPERPVSAAEALRKTSS"
     gene            1140..1646
                     /locus_tag="VFX46_07840"
     CDS             1140..1646
                     /locus_tag="VFX46_07840"
                     /inference="COORDINATES: protein motif:HMM:NF018482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX5600532.1"
                     /translation="MRGFFRISATAVFAIASAASALAQTRLVDTFNDWYFYSHEGSGE
                     RVCFAASAPQSSEPAGINREPAYFYISAWPKEGVRTELSVKIGYQFKEGSEAKVTIGN
                     ETFTMFTHEDRAFVNNPIEELKLLEAMKRGSTMVVEGVTEQGATTRDTFSLMGVMKAV
                     QNLTSQCG"
     gene            complement(1649..2125)
                     /locus_tag="VFX46_07845"
     CDS             complement(1649..2125)
                     /locus_tag="VFX46_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX5600533.1"
                     /translation="MDRVDTPPEASTQPLRSSPSADRPPASREPPPLAEFDRRQVRRI
                     ATGRTAIDARIDLHGMRAAEARAALRSFIFSCYARGQRNLLVITGKGDDVERRDLPFE
                     LFERSGRGVLKRSVPGWLAEPELRSIVISYTQAHPRHGGEGALYVQLRSRRTRDRP"
     gene            complement(2299..>2609)
                     /locus_tag="VFX46_07850"
     CDS             complement(2299..>2609)
                     /locus_tag="VFX46_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX5600534.1"
                     /translation="ALGVDGVPLTAGRSLAVDAGIHALGLPIYVVAPDLVHTPEGVPF
                     HRLMIAQDVGSAIKGPERGDIFFGTGAEAGRRAGMTRHAGNFFVFLPRKCSRASVQVP
                     "
BASE COUNT          517 a          837 c          765 g          490 t
ORIGIN      
        1 cggccactgt ctcgccagct tgcagccggc cccgttcctt gaggccgtgg atagcggttc
       61 cgtaggtcac gttaatgccg gcggcaacct cgtagctgac gccctcgggc acgggactaa
      121 gctgctcctc gcgcacgatc acaaattcgc gcgcaccgcc ccaaccgaga taggcgcata
      181 cccgctggcc cggggagaat ccttccacgc cctcgcccaa ggtttcaatt tcaccggcaa
      241 tctctgcgcc tggagagaac ggcagctcag gcttatgctg gtatttcccg cgcgtcatga
      301 gcgtatcgag gaagttgatt ccgacagcct tcacgcgcac cctgacctca cctggaccgg
      361 gctccggcac gggcaagtcc tcgatcacga tcccctcggg tccgatcagg gacttgcaaa
      421 ctgccgctcg catgtgacac ctcctgctcc atcgcgttcg ctaattcgcg ataacgtata
      481 ggcaatttgc ccatcccggc agcccatttg gctgcaatcg acgagagaga tgaacgaacg
      541 cacccctaat tggctacgcg agaactggcc accgaaacca cgcgggtatt tcgccatagg
      601 cgccgagcgc atctcgaagg cgctcaatct cggcaatctc atgcgctccg cccatgcgtt
      661 cggtgccagc ttcaccttca cgatcggagc aacctaccag gcgctcgagg cacgagccga
      721 cacgtccaaa ggacaatggc acctaccgca ttacaattgg gccaaggtcg aagacctcgt
      781 tctgccagag ggttgcaagc tcgttggcat cgagcttaca gaggacgcca tagatctgcc
      841 gagcttctat catccactac gggcggccta tgtgctcggc ccagagcgag gctcgctgtc
      901 ggaagcgcta cttgcgcgct gtgaccatgt cgtcagaatt ccgacggcgt tctgcatcaa
      961 cgtcgccatg gctggagcca tcgtcatgta cgatcgggtc aagacactgg cgcgctttcc
     1021 ggagcggccg gttagtgctg cggaagctct ccgcaaaaca tcatcgtgac atgcagctcc
     1081 gcttaacact atatgttctc gactaaggtc aagctgtgct tcccattgcg cgaggtgaga
     1141 tgcgagggtt ctttcgcata agtgcaaccg ctgtcttcgc catcgcgagc gccgcatcgg
     1201 cacttgcgca gacacggttg gtcgacacgt tcaacgactg gtatttctac tcccatgaag
     1261 gctcgggcga acgcgtttgc ttcgctgcca gcgcacctca atcgagcgag ccagccggca
     1321 taaatcggga accggcctac ttctacattt cggcttggcc gaaggaaggt gtgcgaaccg
     1381 agcttagcgt gaagatcggt tatcagttta aggaagggtc cgaggccaag gttacgatcg
     1441 gcaacgaaac attcacgatg ttcacgcacg aagatcgtgc cttcgtgaac aatccgatcg
     1501 aagagctgaa gctactcgag gccatgaagc gcgggtcgac gatggtggtc gagggtgtga
     1561 ccgagcaggg cgcgaccacc agggacacgt tttctcttat gggagtaatg aaagcggtcc
     1621 agaatctcac ctcccagtgt ggctgagatt acggacggtc cctagtgcga cggctgcgga
     1681 gctgcacgta gagtgcgcct tcgcccccat gccgtggatg agcctgcgtg tagctgatga
     1741 cgatggagcg aagctcaggc tcggccagcc agccgggtac gctccgcttc aggacgcctc
     1801 ttccggatcg ttcgaatagc tcaaacggaa gatcgcggcg ctccacgtca tcgccctttc
     1861 ccgtgatcac aagcaagttg cgctgcccgc gggcatagca agagaagata aagcttctga
     1921 gggcggcgcg cgcttcagcc gcccgcatgc cgtgcaggtc aatccgggcg tcgatggcag
     1981 tccgcccagt tgcaatgcgc cgcacctgcc gccgatcgaa ctccgccagc ggcggcggct
     2041 cacggctggc gggcgggcga tcagcggaag gagaactgcg gagcggttgc gttgatgcct
     2101 ctggtggcgt gtcgaccctg tccatcagac tcgccgacac ctgctgttcg ccgtgttcct
     2161 ccagcgaggg acctgaagac ccatgcacgc gtgacttttt ccgcacgggc tccaaagtgc
     2221 gcgcaacagc ctcccatagc gcccggtcct ctcgtgtgag cgggcgctgc tgcgagcgtg
     2281 gcggtcgccc cggtcggctc atggcacctg tacggaagcc cggctgcact tgcgcggcag
     2341 aaacacgaaa aagttcccgg catgtcgagt catgcctgcg cgccggcccg cctcggctcc
     2401 cgtcccgaag aagatatccc cacgctcagg acctttgatc gcagagccca cgtcctgtgc
     2461 aatcatgagc ctgtgaaacg gaactccctc cggggtatgc acaaggtcgg gcgccaccac
     2521 gtagatcggc agcccgagag cgtgaatgcc tgcgtcaact gccaagctcc gcccagcggt
     2581 caacggcacc ccgtctaccc ccaaggcgg
//
LOCUS       DASUMG010000476         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_546328, whole genome shotgun sequence.
ACCESSION   DASUMG010000476 DASUMG010000000
VERSION     DASUMG010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546328"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1797)
                     /locus_tag="VFX46_07855"
     CDS             complement(<1..1797)
                     /locus_tag="VFX46_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5600535.1"
                     /translation="MAPTDISNPKYFHKVIDCQWACPSHTPVPEYIRLIAQGRYTAAY
                     MLNWESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADYKGEIDSLLPKI
                     PKEKNGKRIALIGAGPASLTVARDLMPLGYHCVLFEKDQQAGGLMRTNIPAFRLPVTV
                     LDEEVNRILNFGVETRFGHEVQSLKAVLDEGFDAVFIGTGAPKGKDLNIPGRKEGAAN
                     IHIGIEFLTSVAFEHVNSIGRRVVVIGGGNTAMDCCRTALRLGAEQVTVTVRSPRKDM
                     KASEWEIEDAEREGIPILDNHNPKEFIVENGRLVGVRFQIMRAEYTDRGRKLIPTGEE
                     VVIPCDDVLMAIGQENAFPWIERNIGIEFGEWDMPKVDRVTMMSTRPGVFFGGDAAWG
                     PENIIWAVAHGHQAAISIHLYCQGKPLTERPAPGWTLVSQKMGIHEWSYSNAYSPERR
                     VAVPHADKSIALRNLKVEVELGYDDKLAAKEVERCLNCDVQTVFTRSLCIECDACMDI
                     CPVDCINFIENAEEAELRERLRVPARNTSQSLYVSDELKTGRVMVKDEDLCLHCGLCA
                     ERCPTGAWDMQKFTYVEAQASPACLTHHNAYLR"
     gene            complement(2330..>2646)
                     /locus_tag="VFX46_07860"
     CDS             complement(2330..>2646)
                     /locus_tag="VFX46_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEX5600536.1"
                     /translation="LWTAAEPVAREWVEANYGVTGRLREAGEGAEVLGKVLSDLPGLL
                     AKAEEGVTAFAEMAKSGIRLDTETVERIGAAEARHTRWERVALWVGALSLAAIALALA
                     TG"
BASE COUNT          525 a          828 c          753 g          540 t
ORIGIN      
        1 tcgaagatag gcgttgtgat gtgtgaggca ggcaggcgac gcttgcgcct ccacatacgt
       61 gaacttctgc atgtcccatg ctcccgtcgg gcagcgctca gcacacagcc cacagtgtag
      121 gcaaagatcc tcatccttga ccatgacccg gccggtcttg agctcgtccg agacatagag
      181 ggattgcgac gtgttccgag ccggcacccg cagtctctcc cgcagctccg cttcctcggc
      241 gttctcgatg aagttgatgc agtcgaccgg gcagatatcc atgcaggcat cgcactcgat
      301 gcacagcgag cgcgtgaaga cggtctgcac gtcgcagttc aggcagcgct ccacctcttt
      361 cgcggcgagc ttgtcgtcgt agccgagctc gacctcgacc ttcagattgc gcagcgcgat
      421 ggacttatcc gcgtggggaa cggcaacgcg ccgctccgga gagtaggcgt tggagtacga
      481 ccactcgtgg atgcccattt tctggctgac gagcgtccac ccaggcgcgg gccgttcggt
      541 cagtggctta ccttggcagt agagatgaat ggaaatcgct gcctggtggc catgggcgac
      601 ggcccagatg atgttttccg gaccccaggc cgcgtcgccg ccgaagaaca cgcccggccg
      661 agtcgacatc atggtcacgc ggtcgacctt gggcatatcc cattcgccga actcgatgcc
      721 gatgttccgc tcgatccagg ggaaggcgtt ctcctgcccg atagccatca gcacatcatc
      781 gcagggaata acgacctctt cgccagtggg gatcagcttg cggccccgat cggtgtactc
      841 ggcccgcatg atttggaacc gcacgccgac gagccggccg ttctcgacga tgaactcctt
      901 cggattgtga ttgtcgagga tcggaatgcc ctcgcgctca gcgtcctcga tttcccactc
      961 cgacgccttc atatccttgc gcggtgagcg gaccgttacc gtgacctgct cagcgccgag
     1021 gcgcagggcc gtgcggcagc aatccatggc ggtgttgccg ccaccaatca ccacaacgcg
     1081 acgaccaatg ctgtttacgt gctcgaaggc aaccgaggtc aggaactcga ttccgatgtg
     1141 aatattggcg gcgccttcct tgcggccggg gatgttgagg tccttcccct ttggggctcc
     1201 ggtgccgatg aagacagcat caaaaccctc atcgaggacc gccttgaggc tctgcacctc
     1261 gtgaccgaag cgtgtctcga cgccgaagtt caggatgcgg ttcacttcct cgtcgaggac
     1321 agtcaccggc aagcggaaag cggggatgtt cgtccgcatg agcccgccgg cctgctggtc
     1381 cttttcgaag agcacgcagt gatagcccag gggcatcaga tcgcgggcaa cggtcagcga
     1441 cgccggccct gcgccaatca aggcgatgcg cttgccattt ttctccttcg gaattttcgg
     1501 aagaagcgag tcgatttcgc ccttgtaatc ggcggcaacc cgcttcaggc ggcagatcgc
     1561 caccggcttt tcctcgacac gccctcggcg gcacgccggt tcacacggcc gatcgcagac
     1621 gcggccgaga attcccggaa agacgttcga ttcccaattc aacatatagg ccgctgtata
     1681 gcggccttgt gcaattagcc tgatgtactc tgggacgggg gtatggctgg ggcacgccca
     1741 ctggcaatct atgaccttgt ggaagtactt aggattggag atgtcagtcg gagccactcc
     1801 tcggctccgc gtgcgcacag cggcgtcgcc ttgcggcata ggaaacttac cctccagtcg
     1861 ttcgagcccc aagccacgtc catagatgcg gcctttctac ctctgtccca acttagttat
     1921 ttcggcccgc aaatggcaag ctctggacag catatcagcc catcaaaact gagatgatcc
     1981 atctccaaat ttcaacttgg atgcacctga ggcgaccgtc aggcaatttt cattcgcagg
     2041 cgcattaatg gacggaattt tgaaacaaga aattcgcaac cgcagcatga gtccatttct
     2101 cgttagttgc ggtgcagcat aatttcaaat tcatcccgcg tagcacaatt acatcggaca
     2161 actcgcccga agctgacaaa acttgtcctt tgctcagctt atctttgatc ttatcaaacg
     2221 agcacatcgg aaattttgta agctggcgct ctataaccag acgttgtgcc gtctgcacga
     2281 agcctggtcg cgccgattcg gagaccagag gatgagctca acagctcggc tagccggttg
     2341 cgagcgccaa cgcgatggcg gccaagctca gtgcaccgac ccataaggcg acccgttccc
     2401 agcgggtgtg gcgggcttcg gcggcgccga tccgctccac cgtttcagta tcgagacgaa
     2461 tgcccgattt cgccatctcg gcgaaggcag tgacaccttc ctcagctttc gcgagcagtc
     2521 ccggaaggtc ggaaagaacc ttgccaagca cctcggcgcc ctcgcccgct tcgcggagcc
     2581 gacccgtgac gccgtaattc gcctcgaccc attcacgcgc gaccggttcg gcagccgtcc
     2641 agagat
//
LOCUS       DASUMG010000477         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_866913, whole genome shotgun sequence.
ACCESSION   DASUMG010000477 DASUMG010000000
VERSION     DASUMG010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866913"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFX46_07865"
     CDS             complement(<1..203)
                     /locus_tag="VFX46_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600537.1"
                     /translation="MIVSSSAPSIATRPARRDEAGLLAELVNYAGEGLPLYLWGKLAS
                     PGKSSWDIGRERAERDEGSFSWR"
     gene            complement(283..1239)
                     /locus_tag="VFX46_07870"
     CDS             complement(283..1239)
                     /locus_tag="VFX46_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX5600538.1"
                     /translation="MSKSSSNRLPQLDGGLFLTDGGIETTLIFHEGIDLPYFAAFHLM
                     KSPEGRAALVRYFEQYVAIARADGTGFIFESPTWRASSDWGALLGYSRNDIAAVNRDT
                     IALMRDLQAKHGVPQLPMVVSGCVGPRGDGYDPGQVMSAEEAESYHAHQISAFAEAKA
                     DMVTAITMTNANEAIGITRAAMKAGMPVAISFTTETDGRLPTGQSLAEAIAEVDEATA
                     AGPAYYMINCAHPNHFMSALPAGESWTKRVRGLRCNASKRSHQELNDSPDLDAGNPVE
                     LGDQYRELLHLHPQINVLGGCCGTDHRHIACISKACRSAMAA"
     gene            1786..>2292
                     /locus_tag="VFX46_07875"
     CDS             1786..>2292
                     /locus_tag="VFX46_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600539.1"
                     /translation="MTAEVVVDPAPRADAAEAAASSPWLLEVDNLHVHFVTTAGVVRA
                     VEGLSYKVKPGEVVALVGESGCGKSVSALTVMRLLAKPAGRVVAGRIMFQGRDLLKLS
                     EEEMRAIRGREISMIFQEPMTSLNPVLTIGQQVAEVLVLHRKLSQREALAQAVEGLRK
                     VQISEPERR"
BASE COUNT          419 a          680 c          766 g          427 t
ORIGIN      
        1 ttgcgccacg aaaagctgcc ctcatcccgc tccgcgcgct cgcggccgat atcccaagac
       61 gactttccgg gactagcaag cttgccccag aggtaaagcg gcagcccttc gcccgcgtaa
      121 ttgacgagct cggcaaggag accagcttcg tcgcggcggg cagggcgggt cgcgattgat
      181 ggcgctgaac tgctgacgat cattcgttgt ctccagaaag ggtcgcccgg aacccggggg
      241 tagaggggga ctggtgggga ccgggtcccg ggcgaagctc actcaggccg ccatggcact
      301 ccggcaggcc ttgctgatgc acgcgatgtg gcggtgatcc gttccacagc agccgcccag
      361 tacgttgatt tgcggatgga ggtgcaggag ttcgcgatac tgatcgccga gctcgactgg
      421 attgcccgca tcgaggtctg gtgagtcgtt aagctcctgg tggctgcgct ttgaagcgtt
      481 gcagcgaagg ccgcggaccc gcttcgtcca gctctcgcca gcaggcagtg cgctcatgaa
      541 gtgattgggg tgagcgcaat tgatcatgta ataggcgggg cctgcagcgg tggcctcatc
      601 cacctcggcg atcgcttccg cgaggctctg gcctgttggc agacggccgt ccgtctccgt
      661 cgtgaacgag atggcgaccg gcatgcccgc tttcatcgca gcgcgcgtaa tgccgatagc
      721 ctcgttggcg ttcgtcatgg tgatggccgt caccatgtcg gccttggcct cggcaaaggc
      781 gctgatctgg tgcgcgtgat agctctcggc ctcctcggcg gacatcacct gcccagggtc
      841 gtagccatca ccccgcggtc caacacaacc gctcacaacc atggggagct gaggaacacc
      901 gtgcttcgcc tgcagatccc gcatgagcgc gatggtgtcc cggttcactg ccgcgatgtc
      961 gttgcgggag tagccgagga gcgcgcccca atctgagctc gcgcgccagg tcgggctctc
     1021 gaagatgaag cccgttccat cagcgcgggc gatggcgacg tattgctcga aatagcggac
     1081 gagggccgcg cgaccctccg gcgacttcat gagatgaaac gcggcgaaat agggcagatc
     1141 gatgccttcg tggaaaatca gcgtcgtctc gatgccgcca tcagtaagga acaagccgcc
     1201 gtcaagctgc ggcaggcgat tgctggagct tttcgacatg ggttttcctg ctgtctcatg
     1261 atcaattcaa acgatttggc gagcgccaaa ttcgcggacg ggcaaaaagc agatccgcgg
     1321 agccttcgct gtttcgtagg gaacaggacc tttcgtcgtt gctgccgcgt ctgcgaacag
     1381 ccggagcgat cggcgagttt ctgccgaggc gagaggccag ggccggaaag agaagccata
     1441 cgggggctgg cttccatgca acttcgccgg aagtttacga gccgacgggt tgccggatgg
     1501 gtcatagggc gctatgcatg cccacgaaat tcagaacctc ttgtgaagac gagggatttg
     1561 gcgagaagtc ggagttggct ggccaccgac aaagcgcggc tacggcgaca aactccggcc
     1621 caggaacgtt gggaggatgg cgggtacgca agtacgcgtc acagcatgag gcgtaatggc
     1681 cagtagtagt gacactcgca cccaatccag acgccccttc agcgattttg ttccttacgg
     1741 tgacacagta gagagccgca ccgagcggcc gggttcggcc cagccgtgac cgcagaagtc
     1801 gttgttgatc ctgcgcctcg cgcagacgcg gcggaggctg ccgcgtcttc tccctggctg
     1861 ctggaggtcg ataatctcca cgtccacttt gtgacgacag ccggtgtggt tcgcgcagtc
     1921 gaggggctgt cctacaaggt taagccaggt gaggtggtgg cgctcgtcgg cgagtccggc
     1981 tgcggaaaat ccgtgtcagc gctcaccgtc atgcgtttgc tcgccaagcc ggcgggtcgc
     2041 gtcgtcgccg gccggatcat gttccagggc cgcgatctcc tgaagctcag cgaagaggag
     2101 atgcgcgcca ttcgcggccg ggagatctcg atgatcttcc aggagccgat gacctcgctc
     2161 aacccggtgc tcacgatcgg ccagcaggtt gccgaagtgc tggtgctgca ccgcaagctt
     2221 tcgcagcgcg aggcgctggc gcaggcggtg gaaggcctgc gcaaggtgca gatttccgag
     2281 cctgagcggc gg
//
LOCUS       DASUMG010000478         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_65065, whole genome shotgun sequence.
ACCESSION   DASUMG010000478 DASUMG010000000
VERSION     DASUMG010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65065"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /gene="lptD"
                     /locus_tag="VFX46_07880"
     CDS             <1..1139
                     /gene="lptD"
                     /locus_tag="VFX46_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEX5600540.1"
                     /translation="ALSFSRSVSHTFNDNKAWTGETAMNRLVAEVNWRRRFIDPLGQT
                     YTPFFNLRGDFYSLHNVLDPVTLDPIDDEIETRGIAAAGILYAYPFVSHSATGAHVIE
                     PLAQVIARNSSGSQRHLPDEDSRSIVFDDTNLFELNKMAGYDRVETGTRANVGLQYTY
                     QANDGWFARVLAGQSYHLAGDNIYRDPGFVPGREAGGPDEVPIYSPYSGLETDRSDYV
                     LAAYLAPSSVFRTIAQTRFDEKDFSLRRADLFGEIKYGPLIAQTTYSYATFDPFNGER
                     TEQEDIFAVAGLQLTDRWSLLGSIRYDLDAGATVQDALQLRYADDCFVLTATYTETFI
                     NDPLRQLEEDRTLMLRFELKHLGEFHYKTDALDFFFAENQTEPP"
     gene            1443..2636
                     /locus_tag="VFX46_07885"
     CDS             1443..2636
                     /locus_tag="VFX46_07885"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX5600541.1"
                     /translation="MRVPPSEGDSAAGTGRAAPADKGQARPKASAKTKSKSASLDSSG
                     SGKRRGSVQSIVVLVNDDPITAYQVDQRARLLAVQSDIGERAQANFKRLIQQDSTNQR
                     LRQILQETIQANQGKSREEILAIFERRKQQFAEQLQRQAVESARSSLVSSHRKKALEE
                     LIEERLKLQEAKRLGISIDESQVDEIIQNIAQRNNQTPQQFAQNLKRMGADIESMRAR
                     FRATLAWNAVVRRRFSAQVAVSQAEIDRMVSSASGGGQDQVELHLHRITLPIEGSLNQ
                     KVMAQRLDQAERIWRSFRGCNTTAAVAQSQGAKFEDLGMRKPSSVPEPTRSLLLEAKD
                     GQMVPPNMSSQGVELYAVCGRKVTRGDDKVREQVAQELQQKEFEVLAQRHLRDLRQDA
                     HIEYR"
     gene            2640..>3388
                     /locus_tag="VFX46_07890"
     CDS             2640..>3388
                     /locus_tag="VFX46_07890"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEX5600542.1"
                     /translation="MHSHTPDRTAPPLLALTMGEPAGIGLELSLKVWSQARTERPAPA
                     YRLVLYADPAEVVASARRLGMPDPSKVVSAPAEALGLWPEHLPVVAVPLQRPVEPGRP
                     DPANAPAVIGAIEQAVAAVVRGEAAAVVTNPIAKSVLYKAGFTHPGHTEFLADLASRL
                     VGNGPWRPVMLLASDELRVVPLTIHIPLAEVPKALSQELIVETACILNDALVRDFGLG
                     VERRPRIAVTGLNPHAGEDGAMGTEEQTIIAP"
BASE COUNT          681 a         1069 c         1003 g          635 t
ORIGIN      
        1 atgcattgag tttctcacgg tcagtttcac acacgttcaa cgacaacaag gcttggactg
       61 gagaaacggc gatgaaccgc ctcgtcgccg aagtgaactg gcggcgtcgc ttcatcgatc
      121 cgctcggcca gacctacacg cctttcttta atctccgggg cgacttctat tcactgcaca
      181 acgtgctcga cccggtaact ctcgatccga tcgacgacga gatcgaaaca cgaggcatag
      241 ccgccgccgg tatcctctac gcctatccgt tcgtgtcgca ttcggccacc ggagcacatg
      301 tgatcgagcc tctcgctcag gtcatcgcac gcaacagcag tggctctcag cgtcatctgc
      361 ccgacgagga ttcgcgcagc atcgtcttcg atgacacgaa cctcttcgag ttgaacaaga
      421 tggctgggta cgaccgcgtt gagactggga cgcgagcgaa cgttggcctg cagtatactt
      481 accaagccaa cgatggctgg ttcgcccgag tgctcgctgg gcagagttat catctggccg
      541 gtgacaacat ctaccgcgat ccaggtttcg tgcccggtag agaggctggt gggccggatg
      601 aagtacctat ctactcgcct tacagcggcc ttgaaaccga tcggtctgat tacgttcttg
      661 ccgcttatct tgccccctcg tccgtgtttc ggaccatcgc ccaaactcgc ttcgacgaga
      721 aagatttcag cttgaggcgg gcggacctct ttggcgaaat caagtacggt cctctgatcg
      781 cccagaccac ctacagctac gcgacgttcg atccgttcaa tggcgaaaga actgaacaag
      841 aggacatctt tgccgttgct ggtctgcagc tgacggatcg ctggagcttg cttgggtcga
      901 ttcgatacga cctcgatgct ggcgcaaccg tgcaggatgc tttgcagttg cggtacgccg
      961 acgattgctt cgttttgacc gccacctata cggaaacctt catcaacgac ccgctgcgtc
     1021 agcttgagga ggatcgcacg ttgatgctcc gcttcgagct gaagcacctc ggcgaattcc
     1081 actacaaaac ggacgcgctc gacttcttct tcgccgagaa ccaaaccgaa ccgccgtagt
     1141 tgggcgttcg caacacggcg atctttcctc cgcagcgacc agcctttcac cgcttaagcc
     1201 tcgctcgccg ttgccttccc atcgccactt acggtgtgga tacttcgggt gccggcatca
     1261 gctagccgct atgattgatc gtgattcgat ctggcagccc tctaatgatc ggatgaggaa
     1321 tgaggcaagg ctacacaccg aaccgcgcaa acgtccgcct tccagctctc gctctgacgg
     1381 cagccctgct cctcccggcg gttgcgtacg cacaaggctc tcagtccctg ccaggggtca
     1441 tcgtgagagt gccaccgagc gagggggact ccgcggcggg aaccggtaga gcggcgcccg
     1501 cggacaaagg gcaggcacgg cccaaggcca gtgccaaaac gaagtcgaag tcggcctcac
     1561 tcgactcgag cggttccggc aagcgtcgcg gctccgtcca gtcgatcgtc gtgctcgtca
     1621 acgatgaccc tatcaccgcc taccaagttg accaacgtgc gcggctgttg gcggttcaat
     1681 ctgacatcgg ggaacgcgcc caggccaact tcaagcgcct gatccagcag gacagcacga
     1741 accaacggtt gcggcagatc ctccaagaga cgatccaggc taaccagggt aagtcgcgcg
     1801 aagagatcct tgctatcttc gagcggcgca agcagcagtt tgccgagcag cttcaaaggc
     1861 aggcggtgga gagcgccagg tcttccctcg tgtcctccca tcgcaagaag gcgctcgaag
     1921 agctgatcga ggagcggctg aagctccagg aagccaagcg tctcggaatc tcgattgatg
     1981 agagccaagt cgacgagatc atccagaaca tcgcccagcg caacaaccag acgcctcaac
     2041 agttcgccca gaacctgaag cggatgggcg ccgacatcga gtccatgcgc gcccggttcc
     2101 gcgcgacgct ggcctggaac gcagtggtcc ggcggcggtt cagtgcgcag gttgcggtaa
     2161 gtcaggccga aatcgatcgc atggtgtcga gcgcttctgg cggtggccag gaccaggtcg
     2221 agttgcatct ccacagaatc acgctgccaa tcgagggcag cttgaaccag aaggttatgg
     2281 cccaacggct cgaccaggcc gaacggatct ggcgcagttt caggggttgc aatacgacgg
     2341 cggcggtcgc ccaatcccag ggcgcgaagt tcgaggatct cggaatgcgt aagccgtcgt
     2401 cagtgcccga gccaacacgc agcctcctcc tcgaggccaa ggatgggcaa atggtgccac
     2461 cgaacatgtc gagccaaggg gtcgagcttt acgcggtctg cggccgcaag gtcaccaggg
     2521 gcgacgacaa ggttcgcgag caggtggctc aggaactgca gcaaaaggag ttcgaggttc
     2581 tggcccaacg ccatctcagg gatctgcgcc aggacgccca tatcgagtat cgttgaacgg
     2641 tgcattctca tacacctgac cggaccgcac ctccgctgct tgccttgaca atgggcgagc
     2701 cggccggcat cgggctcgag ttgtcgctca aagtctggag ccaggcccgt acggaaagac
     2761 ccgctccggc gtataggttg gtgctctacg ctgatccggc cgaggttgtg gccagcgctc
     2821 gccgcctcgg catgcccgat ccgagcaaag ttgtttcagc gccagccgaa gccctgggtt
     2881 tatggccgga acacctgccc gtcgtcgcgg ttccccttca gcgtcctgtc gagcctggcc
     2941 gcccggatcc cgccaacgca cccgcggtga tcggcgcgat cgagcaagcc gtcgcggctg
     3001 ttgtccgcgg tgaagcagcg gcagtggtga cgaaccccat tgccaagagc gtgctctaca
     3061 aagccggctt cactcatccg ggccacaccg aattcctggc cgatctggcg agccgcctgg
     3121 tgggcaacgg cccctggcgc ccggtgatgc tgctggcctc cgatgagctt cgggtcgtgc
     3181 ccctcaccat tcacatcccc ttggcggaag ttccgaaggc cctcagccag gagcttattg
     3241 tcgagaccgc gtgcattctg aacgacgcac tcgtccgcga cttcgggcta ggtgtggaac
     3301 ggcggccccg gatcgccgtg acgggtctca atccccatgc cggagaggac ggagcgatgg
     3361 gcaccgagga gcagaccatc attgcacc
//
LOCUS       DASUMG010000479         2076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_386672, whole genome shotgun sequence.
ACCESSION   DASUMG010000479 DASUMG010000000
VERSION     DASUMG010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386672"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1324
                     /locus_tag="VFX46_07895"
     CDS             <1..1324
                     /locus_tag="VFX46_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600543.1"
                     /translation="AAYASVLEARRAELHYRTAEALAKDERAADEAPEIIARHYAAAG
                     DPKKALTWWLKAGARASEMAATRSAVHHLTQALAVIRQNPGATNPQEAVAVLRLLGAQ
                     LVSLKGNAAPEAVETFRRCLDLSREAPAADGEFDALWALHTCHLVRGEITEALAVGER
                     LTDKANRDGSEERRLRAHRVQGLAKLLNGQLDDAFRHLRLALDLYDEARHASLRFQYA
                     SDQGALARAHLAWGEAIAGDIVSSNRNAEAALALSSRLRHPHTSAHVLCVLAARAQTL
                     GERATASALAYAGRVIAERHEFPYWIAWADIILGWSQAYDLGYEAAIHQIEKAIGAYR
                     RTGAGQALPYALLLLADVALKYDRPHRALSAATEGWQLAEQYGIMLYAAELLRLRAVA
                     KIRSGSELGDVSQILEQAEQLAARQGAKTFFCRIAEFRLHFDRRQMVS"
BASE COUNT          402 a          612 c          675 g          387 t
ORIGIN      
        1 ggcagcatat gcctcggtcc tcgaggcgcg acgcgccgag ctccactatc gcaccgccga
       61 agccttggcc aaggacgagc gtgcggcgga tgaggcccca gagatcattg cccggcacta
      121 cgcggctgcg ggcgacccaa agaaggcatt gacatggtgg ctcaaagcgg gcgcgagggc
      181 ttccgagatg gctgcgacga ggtcggccgt ccaccacctc actcaggcgc ttgccgtcat
      241 tcggcaaaat ccaggcgcta cgaacccgca ggaggcggtg gcagtgctcc gcctgcttgg
      301 cgcccagctc gtgtcgctga agggaaacgc cgcaccggag gctgtcgaga ccttccggcg
      361 ctgccttgat ctctctcgtg aagcaccggc tgctgatggc gagttcgacg ccttatgggc
      421 tctccacacc tgccatctcg ttcgcggaga gatcaccgag gcgctcgccg ttggggagcg
      481 tctgacggac aaggctaaca gggacggcag cgaagagcgc cgtctgcgcg cgcaccgggt
      541 tcagggtctc gccaagctcc tgaacgggca gctcgatgac gctttccggc accttaggct
      601 ggcgctggac ctctacgatg aggcccgtca cgcgagcctc aggttccagt acgcatcgga
      661 ccagggtgct ttggcccgag cccaccttgc gtggggcgag gcgatcgcgg gggacatcgt
      721 tagctccaac cggaatgccg aggcggccct cgcgctgtcc tcccggctgc ggcatcctca
      781 caccagcgcg cacgtgttgt gcgtccttgc ggcgcgggcg cagacgctcg gtgagcgagc
      841 gaccgcatcc gccctcgcct acgctggcag ggtcatcgcc gagcggcacg agttccccta
      901 ctggatcgcc tgggcggata tcatcctcgg ctggagccaa gcctacgacc tcggctacga
      961 ggccgccatt caccagatcg agaaagcgat cggggcgtat cgccgcacag gcgctggcca
     1021 ggccctcccc tacgcgctcc tacttttggc cgatgtggcg cttaagtatg atcgtccgca
     1081 ccgtgccttg tcggctgcca ccgaagggtg gcagctcgcc gagcagtatg gcattatgct
     1141 ctatgcggcc gagctgctgc gccttcgcgc agtggccaaa attcggtcgg gatcggaact
     1201 gggcgacgtt tcacagatcc tggaacaggc agagcaattg gcagctcgcc agggtgcgaa
     1261 aacatttttc tgccggattg cggaatttcg gctacacttt gatcggcggc aaatggtttc
     1321 ttaacgggaa acccgaaacg tctgacaccg gatatcaagc tccggaattt gcgcaataat
     1381 tcggcatttt tgctttgaag gcgggcgttg ggatgtgccg tcgggaacct cagaagcctc
     1441 gccgcgtcct tcactttaga cgtgattgcg cagcgttctg atacccggaa tcagtttatg
     1501 gtcgagatgg atgaaacgat cgcatctgcg gtcgagacag cagggtatcg ggccttgacg
     1561 ccggacgggg tgcagcgacg ataccgagcg gcgccaattg gcactttggc cgaaccaaaa
     1621 agtgcgagcg tgcgtttgac tagctgcgtg gagcagaccg gaaagccgtt cattaacatc
     1681 ggtggatcag ccgtcgcagt gcggcacgat cgttgcagcg acccacttct gccagaagtg
     1741 ggcgctaggg tggccggcct attgcgtgct ggctcgggga gaaatcgaat ttcatttggc
     1801 cgcaatcgag atgtagcgcg actgtgttag gatgacacaa ggcggtgatg aagaagtgat
     1861 ggcgcttgaa gcgggcaagc gcattgccaa gtggtttgaa agagcccact tcaagcttga
     1921 taggtgatgg cgggaggcca cgtcatcacg agagtggtga gatcgcgaga gcgagaacaa
     1981 gcatgacctc cgcagacgag atttttagcc tctacgcgca gagttacgat cgtcagaagc
     2041 agcacgagat gacgctgcgg cagtatctcg aggcct
//
LOCUS       DASUMG010000480         5980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_706959, whole genome shotgun sequence.
ACCESSION   DASUMG010000480 DASUMG010000000
VERSION     DASUMG010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706959"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFX46_07900"
     CDS             <1..515
                     /locus_tag="VFX46_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX5600544.1"
                     /translation="KAKRLVEHKRDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDSN
                     ALQKPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNCEVVLDRKAS
                     DKRIFPAIDIARSGTRKEELLVPRDQLKKMYVLRRILSPMGTIDAIEFLTDKLRQTKN
                     NAEFFQSMNA"
     gene            547..1278
                     /locus_tag="VFX46_07905"
     CDS             547..1278
                     /locus_tag="VFX46_07905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX5600545.1"
                     /translation="MSNHVNAFGQPIGFPVAHWTPRPRPSRITLEGRVCRLEPLSPER
                     HTLDLYEANQADDGRGWTYMAYGPFASLEEYRGWVEKSAASEDPLFYTVIDTASGRAR
                     GVAALMRIDPANGVIEVGNIKYAPALQRTAAATEAMFLLMRYVFDDLGYRRYEWKCDS
                     LNEPSRAAALRLGFTFEGIFRQAVVYKGRNRDTAWFSIIDKEWPALKQAYEAWLAPEN
                     FDPDGRQRRRLSDLIAEKRAAGSGK"
     gene            complement(1267..3312)
                     /locus_tag="VFX46_07910"
     CDS             complement(1267..3312)
                     /locus_tag="VFX46_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX5600546.1"
                     /translation="MTRNRLDRETSPYLLQHKDNPVHWWPWGPEALAEAKRTNKPILL
                     SIGYAACHWCHVMAHESFEDEATAALMNDLFINIKVDREERPDIDAIYMNALHSLGEQ
                     GGWPLTMFLDPDGRPFWGGTYFPPQPRWGKPAFQHVLREVARIYREEPEKVHHNAALL
                     TQALQRNGAGVSDFEISDHLLQELTARMVRAVDPVNGGIYGAPKFPQWNFFWLLWRGG
                     IRYDMDDAKAAVEKTLTQICQGGIYDHLGGGFARYAVDERWLVPHFEKMLYDNALLID
                     LMTEVYRETGNPLFAQRIRETVAWLEREMIAEGEGFAASLDADSEGEEGKFYVWSAEE
                     IDEVLGPEDGRFFREVYGVSPEGNWEGKNILNRLGTIDLLSEEEERRLDGLRAKLLNR
                     RAGRVRPGWDDKVLADWNGLMIAALASASFVFDEPRWREMAERAFAFVEERMMSDGRL
                     VHAYRAGQAKAPATANDYANMIWAALRLCQVTKKSRYLDAAIRLTEQLDRHYWDGGSG
                     GYFLTADDTPDVIVRIKSATDDATPNANTMMISNLPQLFMLTGKTEYLDRAEAILKAF
                     AADLQTNLVAHSGLLANALDFLSPQHVAVLSGGDSKGASALEAVVTSLSLPGAIQHFV
                     EDAEALSGATALVGKRPVEGKATAYVCVGQKCSSPITDPEALRFALRSQRLARPSLA"
     gene            3380..4720
                     /gene="mnmE"
                     /locus_tag="VFX46_07915"
     CDS             3380..4720
                     /gene="mnmE"
                     /locus_tag="VFX46_07915"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX5600547.1"
                     /translation="MSQVATIFALSSAPGRAGVAVIRISGPAAGTVLDTLAAPRPEPR
                     SAKIRYLRRPETGEILDQALVLWFPAPRSFTGEDVAELHVHGGRAVVQAVLRAIGDIP
                     GCRLAEPGEFARRAFENGKLDLTMAEGLADLIDAETEAQRKQALRQAGGALANLYDGW
                     RNSLIEALALVEAAIDFSDEGDVGSETYDQAVRVVRDLEAAIRRHLADGRRGEILREG
                     FNVVLAGPPNVGKSSLLNALARREAAIVSEEAGTTRDVIEVRLDLEGLPIVVGDTAGI
                     REATGAVEREGIRRTLARTREADLVLWLMDAIAPDPNLPSELTDLADRTLWVLNKIDL
                     CPDVGLPGVPENAIAISALTGQGIDVLTRRIAELARDRIAPTEHPGLTQERHRRNLED
                     CRKALAAFLEGSPWEVELRAEDLRLAANALGRLTGRVDPEEVLDGIFGRFCIGK"
     gene            4928..>5980
                     /gene="mnmG"
                     /locus_tag="VFX46_07920"
     CDS             4928..>5980
                     /gene="mnmG"
                     /locus_tag="VFX46_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX5600548.1"
                     /translation="MTEHTYDVVVVGGGHAGTEAAAAAARMGARTALVTHKFATIGEM
                     SCNPAIGGLGKGHLVREIDALDGLMGRVADQAGIQFRLLNRSKGPAVQGPRAQADRAL
                     YRKAMQRAIAETPGLTVIEGSVDDLIVDNGMVQGVVLADGSVLKSGAVVVTTGTFLRG
                     LIHIGEVKIPAGRVGEAPSVRLAERFAALEFRLGRLKTGTPARLLRSSIDWERLEIQP
                     GDDPPVPFSFLTERITTPQVVCHVTNTTEETHAIIRANLHRAPMYSGQIQSVGPRYCP
                     SIEDKVVRFADRTAHQIFLEPEGLDDDTIYPNGVSTSLPEEVQVAFLRTIPGLENVVI
                     KRPGYAIEYDYVDPREL"
BASE COUNT         1121 a         1780 c         1874 g         1205 t
ORIGIN      
        1 agaaggccaa gcggcttgtc gagcacaagc gggacgttgt gatccttctc gactcgatta
       61 cccgcttggg tcgtgcctac aacacggtgg tgccgtcttc gggcaaggtg ttgaccggcg
      121 gtgtcgacag caacgctcta cagaagccga agcgcttctt cggtgcagcc cggaatatcg
      181 aggaaggcgg ttcgctcacc atcatagcga ccgcactcat cgataccgga tcgcgcatgg
      241 acgaagtcat tttcgaggag ttcaagggca ccggcaactg cgaggtcgtg ctcgatcgta
      301 aggcgtcgga caagcgcatc ttcccggcga ttgacatcgc ccggtcgggc acgcggaagg
      361 aagagttgct cgtcccacgc gaccaactca agaagatgta cgtcctccgg cggatcttga
      421 gcccgatggg cacgatcgat gccatcgagt tcttgacgga taagctccgc cagacgaaga
      481 acaacgctga gttcttccag tcgatgaatg cctaagctca gcgccagctg agcagccggg
      541 ggtatcatga gcaatcatgt caacgctttc gggcagccga tcggttttcc ggttgctcat
      601 tggacccctc ggccgcggcc gtcccgcatt acgctggaag ggcgagtttg ccgcctagag
      661 ccactcagcc ctgaacggca cacgctggac ctttacgagg cgaatcaggc cgatgatggc
      721 cgcggctgga cctacatggc ctacggccca tttgcctcgt tggaggagta ccgcggttgg
      781 gtggagaagt ccgcggcgtc tgaggatccg ctcttctaca cggtgatcga cactgcatct
      841 ggtcgcgcaa gaggtgtcgc ggccttgatg cggatcgatc ccgcgaacgg tgtcatcgaa
      901 gtcggcaaca tcaagtatgc tccggccctg cagcgcacgg ctgcggcgac cgaggcgatg
      961 ttcttgctga tgcggtacgt cttcgatgat ctcggctacc gccggtatga gtggaagtgc
     1021 gattctttga atgagccatc ccgcgcggcg gcgcttcggc ttggcttcac gttcgagggc
     1081 atcttccggc aggccgttgt ttacaagggc cgcaatcgcg acactgcgtg gttctcgatc
     1141 atcgacaagg agtggcccgc gctgaagcag gcctacgaag cctggctagc gccggagaac
     1201 ttcgaccccg acggacggca gcgtcgccgc ctctccgatt tgattgcgga gaagcgggcg
     1261 gcagggtcag gcaagtgagg gcctcgccag ccgctggctg cgaagcgcga acctcaacgc
     1321 ctcgggatcc gtgatcggcg acgagcactt ctggccaacg cagacatagg ccgtggcctt
     1381 gccttcaacg ggtcgcttcc ccacgagcgc tgtggcaccc gaaagcgcct ctgcatcctc
     1441 cacgaagtgt tgtatggccc ctggtagcga aaggctggtg accacagcct caagagcgga
     1501 ggcacccttg ctatcaccgc cgctcaacac cgccacgtgc tggggtgaga ggaaatcgag
     1561 agcattggcc agcaggcctg aatgggcgac caggttggtc tgaaggtctg cggcgaacgc
     1621 tttcaggatg gcttctgcgc gatccaagta ctccgtcttg ccggtcagca tgaagagctg
     1681 cggcagattg gagatcatca tggtgttcgc gttgggcgtc gcatcatcgg ttgcggactt
     1741 gatgcgcacg atgacgtctg gcgtgtcatc ggcggtgagg aaataacctc ctgacccgcc
     1801 atcccagtag tgccggtcga gctgttcggt gagccggatc gcagcatcga gataacggct
     1861 tttcttggtg acctggcaaa gccggagggc cgcccagatc atgtttgcgt aatcgttcgc
     1921 ggttgctggg gccttggcct gacctgcgcg ataggcgtgc accagtcgcc catcgctcat
     1981 cattcgttct tcgacgaaag cgaaggcccg ctcggccatc tctcgccagc gtggttcatc
     2041 gaacacgaac gacgctgagg cgagggccgc gatcatcaag ccgttccagt cggcgagcac
     2101 cttgtcgtcc caaccgggac gcacgcgtcc cgcgcgccgg ttgagtagct tggcccgcaa
     2161 cccatcgaga cggcgctctt cctcttcgga taggaggtcg atggtgccga gcctgtttag
     2221 gatgttcttg ccttcccagt ttccctcggg ggagacgcca taaacttccc ggaaaaagcg
     2281 gccgtcctcg gggccgagca cctcgtcgat ctcttccgcg ctccaaacgt agaactttcc
     2341 ctcttcgcct tcggagtctg catcgagcga ggcggcaaag ccctcaccct cggcgatcat
     2401 ctcgcgctcg agccaggcga cggtctcccg aattctttgt gcgaagagtg ggttgccggt
     2461 ctctcgatag acctccgtca tcaggtcgat gaggagggca ttgtcgtaga gcatcttctc
     2521 gaaatgcggg acaagccacc gctcatcgac cgcataccgc gcgaagccgc cgccgaggtg
     2581 atcgtaaatg cccccttggc agatctgagt cagagttttc tcaaccgcag ccttcgcatc
     2641 atccatgtcg taccgaatcc cgccccgcca caggagccag aagaagttcc attgcgggaa
     2701 cttcggtgcg ccgtagatgc cgccgttgac tggatcaacg gcacgaacca tgcgggcggt
     2761 cagctcttgc agcaggtgat cggagatttc gaaatcgctc acgccggcac cgttgcgctg
     2821 taacgcttgt gtcagcagcg ctgcattgtg atgcaccttt tccggctctt cgcggtagat
     2881 gcgcgccacc tcgcgaagga catgctggaa cgccggcttg ccccaccggg gctgcggcgg
     2941 aaaatacgtt ccaccccaga aaggccgccc atcggggtcc aggaacatgg ttagcggcca
     3001 tccgccctgt tccccgaggc tgtgcagggc attcatatat atcgcgtcga tgtctggccg
     3061 ctcctcgcga tccactttga tgttgataaa gagatcgttc ataagagcgg cggtcgcctc
     3121 gtcctcgaag ctctcgtgtg ccatgacatg gcaccagtgg caggcggcat aaccaatcga
     3181 cagcaggatc ggcttgtttg tccgcttggc ctcagccagt gcctctggcc cccagggcca
     3241 ccagtggacc gggttgtcct tgtgctgaag caggtagggg ctcgtctctc gatccaagcg
     3301 atttctggtc atggtactcc ctcctgctgt tgatggagac gagctctcgt ctccggttac
     3361 atagggtgac tccaatagaa tgagtcaggt tgcaacgatt ttcgcgctgt cgagcgctcc
     3421 aggccgtgca ggcgtagccg ttatccgcat ttcgggaccg gcggcgggga cagtgctcga
     3481 tacgttggct gctccgaggc cagaaccccg atctgccaaa atccggtatc tgcgccggcc
     3541 ggaaacaggt gaaattctcg atcaggcact cgtcctctgg tttccggcac cgcggagctt
     3601 cacgggcgaa gatgtcgcag agttgcacgt ccatggcggg agggccgtcg ttcaggcggt
     3661 tctccgagcc ataggtgaca tcccgggctg ccggcttgcc gagccgggcg agttcgccag
     3721 acgggctttc gaaaatggca agctcgacct caccatggct gagggcttgg ctgatctgat
     3781 cgatgcagaa actgaggccc aacgaaagca ggcactacgg caggccgggg gagcactggc
     3841 gaatctttac gacggctggc ggaattcgct gatcgaggcg ctggcgctcg tcgaggcggc
     3901 tattgatttt tcggacgaag gggacgtcgg cagcgaaact tatgaccaag ccgttcgggt
     3961 ggtgcgggat ctcgaggccg caattcggcg gcatctcgcc gacggccgtc ggggcgagat
     4021 cttgcgcgag gggttcaatg ttgtgcttgc tggcccgccg aatgtcggga aatcgagtct
     4081 gctcaacgcc ctggcgcgcc gggaagctgc gatcgtttca gaagaagccg gtacgacgcg
     4141 cgatgtgatt gaggttcgcc tggatcttga gggcttgccc atcgttgttg gcgacaccgc
     4201 cggcatccga gaggcgacag gcgccgtcga gcgcgaaggc atccgccgga ctttggcccg
     4261 gacacgcgag gcggacctcg tgctttggct catggatgcc atcgcgcccg atccgaacct
     4321 cccatctgag ctaacggatc tggcagaccg aacgctgtgg gtgctgaaca agatcgatct
     4381 ctgtcccgac gtcgggcttc ctggggtgcc cgaaaacgca atagctattt ctgcgctgac
     4441 cggtcagggt attgatgtgt tgacccgacg aatagcagaa cttgctcgtg accgtatcgc
     4501 tccgacggag catcctggac tgacacagga acggcatcgc cgaaatctcg aggactgccg
     4561 caaggcgctc gcggcattcc tcgaaggaag cccctgggag gtcgagctca gggccgagga
     4621 tctcaggctg gcagcgaatg cgctgggcag gctcaccggc cgcgtcgatc ccgaggaggt
     4681 tctggatggg atcttcggcc gcttttgcat cggaaaatag agccagctcc gaatgtttca
     4741 cgtgaaactt cacgtattct ggcgatcatt ttcgaaaact tcatttcgtc gatggatggg
     4801 gcagagctgt cactttcgga ggacgtcgcc aagtattccg gtgaagctct actttacgat
     4861 gacttaccgc ctcggttagt gtgcaggccc tcgatctgcc gatggtcgcg ttccttggaa
     4921 aggtgaaatg actgaacaca cctacgatgt tgtcgtcgtt ggaggtgggc atgccggcac
     4981 cgaggccgcc gctgcagccg cccgtatggg cgcgcgaacg gcccttgtga ctcataagtt
     5041 cgccaccatc ggcgaaatgt cttgcaaccc ggcgatcggc ggactcggga aaggtcacct
     5101 tgttcgcgaa atcgatgcac tcgatggcct gatgggccgg gtcgcggacc aggccgggat
     5161 tcagtttcga ttgctcaacc ggtcgaaggg tcccgccgtt caagggccgc gggcgcaggc
     5221 agatcgggcc ctctatcgca aagcgatgca gagggcgata gcggaaacac caggcctcac
     5281 cgttatcgaa ggttcagtgg atgatctgat cgtcgataac ggcatggtgc agggtgtcgt
     5341 cctggcggat ggctctgtgc tgaaatccgg cgccgttgtt gttacgaccg gtaccttcct
     5401 tcggggcctg atccatatcg gcgaagtcaa gattccggct ggccgggtcg gggaggcacc
     5461 ttcggtacgc ctcgccgagc gctttgcggc tttggagttc cgactcggcc gactcaagac
     5521 tgggacaccg gctcggttgc tgcggagctc gatcgattgg gagcgtctgg aaatccaacc
     5581 cggcgacgac ccgccagtgc cgttctcctt cctgacggag cggatcacaa ccccgcaagt
     5641 cgtctgccac gtcaccaata cgacggaaga gacccacgcg atcattcggg ccaatctcca
     5701 tcgtgcgccg atgtattccg gccagatcca aagtgttggc ccacgctact gcccctcgat
     5761 tgaagacaag gttgtgcggt ttgcggatcg gacggcgcac cagatcttcc tggagccgga
     5821 gggcctggac gacgatacga tttaccccaa cggcgtttcg acttctctgc ccgaggaggt
     5881 ccaggtggcg ttcctacgga ccattcccgg attggagaat gttgtcatca agcggcccgg
     5941 ctacgcgatc gagtacgact acgtcgatcc gcgcgagctc
//
LOCUS       DASUMG010000481         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_707150, whole genome shotgun sequence.
ACCESSION   DASUMG010000481 DASUMG010000000
VERSION     DASUMG010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707150"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1768
                     /locus_tag="VFX46_07925"
     CDS             431..1768
                     /locus_tag="VFX46_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEX5600549.1"
                     /translation="MKPIRVAFAAFFLLLTALWLLADTLAPSPFTYFSFRSVFVQYTG
                     VLAIGAMSFAMVLAVRPKWMEPWLDGLDKMYRLHKWLGISALVVGLVHWWWAKGTKWM
                     VGWGWLARPERQPRGQMPDVGAIEAWLRTQRGLAEGLGEWAFYAAVVLIVLALWKRFP
                     YRWFAKTHWWISVVYLVLVFHTIVLTNFEYWAQPVGWVVAALLAAGTGAALLSLFGLI
                     GATRKARGEIEEIIRYPALKVIEVAIRLKDAVWRGHKPGQFAFVTSHENEGAHPFTIA
                     CAWNEQKRLVFIAKALGDFTEKLHEQLQVGMPVTVEGPYGCFTFDGDHPVQIWIGAGI
                     GVTPFIARMKHLANAPEQREIVLFHPTAVYDQTAIDKLSADAKAANVRLIVLVDERDG
                     YLTGDRIREAVSNWREASIWFCGPAKFGQILRADFLAHGFPAEDFHQELFEMR"
     gene            complement(1791..>2006)
                     /locus_tag="VFX46_07930"
     CDS             complement(1791..>2006)
                     /locus_tag="VFX46_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX5600550.1"
                     /translation="GTFYSLDVLPPFWQYVSLFNPIVYLVSGFRWSFYGVADVSVAVS
                     LGMTLVFLVICVVVVWRIFATGYRLKA"
BASE COUNT          340 a          653 c          658 g          355 t
ORIGIN      
        1 cccacgggaa cggaaaaatc ataataggcg gcgctagggg acagaggacc gcggcggggc
       61 ggcgaggagc cgccagagaa aataaaatgc ggagcctcgt cgccccgcgc ccctcacctt
      121 ttccttcccc gcgtgcgcac atgcgcccgc ggatcttcgg cgtgcccgtt tgcccacgcg
      181 cgtgtcaggc acaagtgcga gcccgcctcc ctcgtccagc tccagcgact ccgcgcgtct
      241 ttgcgtcggc ccggggcatc ttgagacgcc ctgttgctgc acgtcccgcg gaattgccaa
      301 aaatcaaacg ggccagagcg gccacgtgca tactgcgcgc cagggatctt ccctgcgttc
      361 cgtcgttacg gtccacggaa gtttcggcgg ccaacaccgc ggtatctctg cccctggcac
      421 ggaaagttcg atgaaaccca tccgagtggc cttcgccgcg ttcttcctcc tgctcacggc
      481 cctgtggctg ctggccgata ccctcgcgcc ctcgccgttc acgtacttct cgttccggtc
      541 cgtgttcgtg cagtacaccg gcgtcctcgc catcggcgcc atgagcttcg cgatggtgct
      601 cgccgtgcgg ccgaagtgga tggagccctg gctcgacggc ctcgacaaga tgtaccggct
      661 ccacaagtgg ctggggatca gcgcgctggt cgtcggcctc gtccactggt ggtgggcgaa
      721 gggcacgaag tggatggtcg gctggggctg gctcgcgcgg ccggagcgtc aaccgcgcgg
      781 gcaaatgccg gacgtcggcg ccatcgaggc gtggctcagg acgcagcggg gcttggccga
      841 ggggcttggc gaatgggcgt tctatgcggc cgtggtgctg atcgtgctgg cgctgtggaa
      901 gcggttcccc taccgctggt tcgccaagac gcactggtgg atcagcgtcg tgtacctggt
      961 gctcgtgttc cacacgatcg tgctgaccaa cttcgaatat tgggctcagc cggtcggatg
     1021 ggtggtcgcg gctctcctgg cggcaggcac gggtgcggcg ctgctctcgc tcttcggcct
     1081 gatcggggcc acgcgcaagg cacgcggtga gatcgaggag atcatccgct acccggcgct
     1141 gaaggtcatc gaggtcgcca tccgcctgaa ggatgcggtc tggcgcggcc ataagccggg
     1201 gcagttcgcc ttcgtcacgt cacacgagaa cgagggcgcg caccccttca ccatcgcgtg
     1261 cgcctggaat gagcagaagc gcctcgtgtt catcgccaag gcgctcggcg atttcaccga
     1321 gaagctgcac gagcagctgc aggtgggcat gcccgtgacc gtggagggcc cctacggctg
     1381 cttcaccttc gacggcgacc acccggtgca gatctggatt ggcgccggca ttggcgtcac
     1441 gcccttcatc gcccgcatga agcatctggc caatgcgccg gagcagcggg aaatcgtgct
     1501 gttccatccg acggcggtgt atgaccagac ggcgatcgac aagctctccg ccgatgccaa
     1561 ggctgcgaac gtgcggctta tcgttctggt cgatgagcgg gacggttatc tgacgggcga
     1621 ccgcatccgt gaagctgttt ccaactggcg cgaggccagc atctggttct gcggccctgc
     1681 gaagttcggc caaatcctcc gcgccgactt cctcgcccac ggcttcccgg cggaggattt
     1741 ccaccaggag ctgttcgaga tgcggtaggc ggccggcgtg agcagttcgc tcacgctttc
     1801 agccgatacc ccgtcgcaaa gatgcgccag acgacgacca cacagatcac cagaaacacg
     1861 agcgtcatgc cgaggctcac cgcgacgctg acatcggcga caccgtagaa gctccagcgg
     1921 aagccgctga cgagatacac gatcgggttg aacaggctga cgtattgcca aaacggcggc
     1981 agcacatcga gcgagtagaa ggtgcc
//
LOCUS       DASUMG010000482         7902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_65657, whole genome shotgun sequence.
ACCESSION   DASUMG010000482 DASUMG010000000
VERSION     DASUMG010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65657"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VFX46_07935"
     CDS             <1..436
                     /locus_tag="VFX46_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX5600551.1"
                     /translation="DVAAVQDALNQIGANAALPDQQTSLPFLVPDGAFGNKTYIRVKD
                     FQAKNGLTQDGIVGPITLGRLQEILPAVQVGMTTSRPAGFVSSGAGGGGGAGGGGAGT
                     GGGAGGGTKTIKFPAAYDAGTLSSSKTSGSGSKTSGYGGKGY"
     gene            691..1677
                     /locus_tag="VFX46_07940"
     CDS             691..1677
                     /locus_tag="VFX46_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600552.1"
                     /translation="MCAPDGQPASGRSFFSPAIKCCTYLPGIPNFLVGRIVTDDGDDI
                     AEGRATIEERIRAGIAVTPFGLGVPAPYQLLYRNAQDFFGRGKGLRCSHYLADQGRCG
                     IWRHREATCATWFCKHEHGATGQQFWAAVRELLRGIERDLARWCVLTLVHDDRALSRL
                     AAADQAEERLDANALDGVPNAAAHRELWGEWVGREPDFFVEATRLVDPLQWSDVLQIC
                     GPAVQIQAAAVRAAHRPLVDYRIPPILRLGRYEVIQVTSNSSTLLTYNEFDPLHVPQR
                     LLDALHFFDGRPTAEACDAIARQRGVVLTSTQLREFIDFRILVAEPEPITEV"
     gene            1742..2290
                     /locus_tag="VFX46_07945"
     CDS             1742..2290
                     /locus_tag="VFX46_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600553.1"
                     /translation="MTTQTAAGLFLALASFAAGQSATKKELPRIANPEVLAIEMAKAL
                     VSADRDRVTALAATGKEMETLLEAAQPPMRPEERKYQKDKVAEILADRRRDFDRFQAM
                     KRKASFGEGVAVRFDLIALDPIYEKDGMKKIRHSRVRMLQGAQGRMAEAFIISLDDMF
                     LFPRGWAFTSISPEIGKEMSRK"
     gene            2373..2446
                     /locus_tag="VFX46_07950"
     tRNA            2373..2446
                     /locus_tag="VFX46_07950"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2407..2409,aa:Arg,seq:tct)
     gene            2495..3028
                     /locus_tag="VFX46_07955"
     CDS             2495..3028
                     /locus_tag="VFX46_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600554.1"
                     /translation="MLKALVVLAVGLAAQVKVPTGLPPIERYGVGSVVIGADAQQVYD
                     AFAGRRELVDLGYEGMLTPALLLRFVGLTQRDGVVAELDSGSLGLVVWRIEIRDPAFR
                     TVKGIGVGSTVGQLKSAYPLDAVLSGEGSVAIRVKALSASFELDQTGPDGSKLSGIRD
                     PALVPASVTIRSVLLTR"
     gene            3275..3817
                     /locus_tag="VFX46_07960"
     CDS             3275..3817
                     /locus_tag="VFX46_07960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX5600555.1"
                     /translation="MIVSIPNSTLGRQSRREGSHVNRFQIISAATVVGIGVSLSARDG
                     QAPSNRPDEDAIKAAINSTTDAFNSHDPRAWLRLATADAELITARGEVMHGTAEIEKG
                     LTALFQGRNKNASVRTLDIRLRLIRPDVALAHVTNEIGGVVDASGQTLPPSRELSSRI
                     LVRDQGVWRMTAFHNTVVQP"
     gene            5319..5807
                     /locus_tag="VFX46_07965"
     CDS             5319..5807
                     /locus_tag="VFX46_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600556.1"
                     /translation="MPHCFGELSGGDVTSAKSTALGVSACMLLAISIGFARQQDRDLS
                     GIHGIPVEGHDVIAGVEIVGTKQLDLSRSGWHKLDERLRANGAQLRPGWPLENQTLCR
                     FKEVLRDVMSEKGFLDVEITHDTRPTYGNPQHLTLKFTIVEGKRSRPAAPVLSPAERC
                     MR"
     gene            6262..6756
                     /locus_tag="VFX46_07970"
     CDS             6262..6756
                     /locus_tag="VFX46_07970"
                     /inference="COORDINATES: protein motif:HMM:NF028114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5071 domain-containing protein"
                     /protein_id="HEX5600557.1"
                     /translation="MRRELRQLLPSNKDDVAAVDSLIALGYPSIEPILPDVLKWIRQP
                     DWPVAPPLSKFLLSLGPSLLPHIHKIFSSPRDDAWKAEILRTIVQYWPREHVAELKSY
                     LAMLATHGNSWGVDLIALRLLARHQLVDRNWIADWLEFKSKFHLDQLQEIESIRKVNS
                     SQAT"
     gene            complement(6834..7385)
                     /locus_tag="VFX46_07975"
     CDS             complement(6834..7385)
                     /locus_tag="VFX46_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600558.1"
                     /translation="MKATLIRAFVIFHKRACQGQVRAWIIGRGLYQVPSGGNLFTKSV
                     LTAALSSTPSATNWNRMKIELTDLVPKSLFRAAERKTIAALDAMSYLIDVEVLGCVAT
                     RAEHLLRDVTFNGMPGKAWLESRIALPPFGLPTVRAGAGVPDQRHIAIQGMLQEAPLV
                     SSLPLLCFACSRSTFGRSLVASH"
BASE COUNT         1622 a         2325 c         2412 g         1543 t
ORIGIN      
        1 tgacgtcgcg gcggtgcagg atgcgctgaa ccagatcggc gcaaacgccg cccttccgga
       61 ccaacaaacg tctcttccct ttctcgtgcc agacggcgcc ttcggcaaca agacctacat
      121 ccgggtgaag gatttccaag cgaaaaacgg actcacccag gatgggatcg tcgggcctat
      181 taccctcggc cgactccagg aaatactgcc ggcggtgcag gttggaatga cgacgagccg
      241 gcccgccggt tttgtgtcaa gtggcgcggg cggtggaggc ggtgcaggcg gtggcggcgc
      301 aggtactggc ggcggcgcgg gcggtggaac taagaccatc aagtttccag cggcatatga
      361 cgcggggacg ctctcaagca gcaagacctc tggctcggga agcaaaacgt ctggctatgg
      421 cggcaagggc tactaaaaaa ttaaccggag gctaccttga ggctgggcta cgcggaagaa
      481 gacacgagcg agcgcagcga agcgagccac gcgaacggag cgagccggcg gagcggctcg
      541 cgagagagcg tgcaagggag tccgaggggc gaagcccctc ggagtgatat cgactccgca
      601 ctgctgccac cgctcttcgg ccgatggctc ggtgaatggc tcaccgagcc ggtacctcgt
      661 gaatcgcacg cgacgtgcga tcgatgcgcg atgtgcgcgc ccgacggtca gcccgccagc
      721 ggccggtctt tcttcagtcc tgcgatcaag tgctgcacct acctgccagg catcccgaac
      781 tttctcgtgg gacggattgt gaccgacgat ggcgacgaca tcgcagaggg acgagcgacg
      841 attgaggagc gcattcgtgc gggcatcgcg gtgacgccat tcggcctggg tgtccctgct
      901 ccgtaccaac tgctctaccg caacgcgcag gacttcttcg gccgtggaaa gggactccga
      961 tgctctcact acctggcgga ccagggacga tgcggcatct ggcggcatcg cgaagccacg
     1021 tgcgccacgt ggttctgcaa acacgagcac ggtgcgactg ggcagcagtt ctgggcggcg
     1081 gtgcgtgaac tactccgtgg gattgagcgc gacctggcgc gctggtgcgt gctcactctt
     1141 gtccatgacg accgcgcgct ctcccggctt gcggccgccg atcaagccga ggaacggctc
     1201 gacgcgaatg cgctggatgg cgtgccaaac gccgccgcgc atcgcgaact atggggcgaa
     1261 tgggtcggac gagagccaga ttttttcgtc gaagctacgc ggctcgttga cccgcttcag
     1321 tggagcgacg tgctgcagat ctgtggtccc gcggtccaga tccaggccgc cgccgttcgc
     1381 gctgcgcacc gtccgctggt cgattaccgg attccgccga tcctgcgcct gggtcgctat
     1441 gaagtcatcc aggtaacgtc gaactcctcc acgctgctca cgtacaacga gttcgatcct
     1501 ctccatgtcc cgcagcgtct tctcgatgcc cttcacttct tcgatggacg gccgaccgcc
     1561 gaagcgtgcg acgcaattgc acgtcagcga ggcgtcgtcc tcacgagcac ccaactccga
     1621 gagttcatcg actttcggat cctggtggcg gagccggaac caattaccga ggtatgaggc
     1681 atccgagcag ccgcccggcg ctggcgtacg gcgcggacgc acaaagcgaa aggaaccaag
     1741 aatgacgacg caaaccgcag ccggtttgtt tctcgctctc gcgtcctttg cggccggcca
     1801 gagtgccacg aaaaaggaac tgcctcgcat cgcgaatccc gaagtgttgg cgattgagat
     1861 ggcgaaggcc ctcgtctctg ccgaccgcga tcgagtcaca gcactcgccg ccacagggaa
     1921 ggaaatggaa acgctgctcg aagcggccca gccgcccatg cggccggagg agcgcaagta
     1981 tcagaaggac aaagtggccg agattcttgc cgatcggcga agagatttcg accgattcca
     2041 ggcgatgaag agaaaggcca gcttcgggga gggcgtggcc gtccggttcg acctgatcgc
     2101 gctggacccc atctacgaga aagacggaat gaaaaagatc cggcattcgc gggtccgcat
     2161 gctgcagggt gctcaaggaa ggatggctga ggctttcatc atctcccttg acgacatgtt
     2221 cctcttccca cgtgggtggg ccttcacttc catctctccc gagatcggaa aggagatgtc
     2281 aagaaagtaa gcgccactgc tcctgacacg ctacacactc tctcgaacgg cgctcgctcg
     2341 caagaacgct gcttgctaca atcgtcttcc atgcgcccgt agctcagttg gatagagcac
     2401 cggccttcta agccgggggt cgcaggttcg aatcctgccg ggcgcgaatt ttccaagtcg
     2461 aaaggacgtt acctaaacac gagagtcgta tgtgatgttg aaagccctag tggtgcttgc
     2521 ggtcggtctg gcggcccagg taaaagtgcc gacaggactc ccgccaatcg aacgctatgg
     2581 tgtcgggtcc gtcgtcattg gtgcggacgc ccagcaagtc tacgacgctt ttgcaggccg
     2641 acgagaactc gtcgatctgg gatatgaagg gatgctgacg cctgcccttc tgcttcgctt
     2701 cgttggacta actcagcgcg acggagtggt tgcggagctt gattccggct cacttggcct
     2761 tgtggtgtgg cggatagaga ttcgtgaccc tgctttccga accgtcaaag gtatcggcgt
     2821 tggttccacg gtaggccagc tgaaatccgc gtatccgctg gatgccgtcc tctcgggtga
     2881 gggcagcgtc gccatccgag taaaggcatt gtcggcaagc ttcgagttgg atcaaacagg
     2941 gccagatggc tctaaattgt cgggaatccg cgatccggcg ttggtgccgg cttccgtaac
     3001 gattagatca gtgcttctca cccgttgaga gttgtctgac aggggaactc tacgctgcaa
     3061 tcctggagaa cggcgttacc aacctttgtt gtaacccgcc gggcgcgata cctcaacagt
     3121 attcggtgtc aaacattgcg ggcgcctgtt gaatgaattt gttgatccca ctgctagcgt
     3181 cgctgatgct accgctcgtt cgctttcagc atgagatggc acccgtctcc tccgataccg
     3241 cacagtggtc ccggtatcgg gaccggtagt acccgtgatc gtgtcgatcc ctaattcaac
     3301 gttgggccgg caatcaagaa gagaaggaag tcacgtgaat cgattccaga tcatttcagc
     3361 ggcgaccgtc gtcggcattg gcgtaagcct ctcggcgagg gacggccaag cgccgagtaa
     3421 tcgcccggac gaagacgcga tcaaggcggc catcaactcc acaactgatg ccttcaatag
     3481 ccacgatccg cgcgcatggt tgcgacttgc cacagcagac gcagagctga ttaccgctcg
     3541 cggcgaggtg atgcacggaa ctgcggagat cgaaaagggg ttgaccgcgt tgtttcaggg
     3601 gcgaaacaaa aacgctagcg tgaggactct ggacattaga cttcgattga ttcggccgga
     3661 tgtagcgctc gcacacgtga cgaacgagat cggtggcgtc gtcgatgcgt cgggccaaac
     3721 gctcccgccg tcgcgggaac tcagttcccg gattctcgtg agagaccagg gcgtctggcg
     3781 catgacagcg ttccacaaca ctgtggttca accctgaacg cgcagcggca acctaaccag
     3841 cgccggagcg ttgcggccga ggtcatctcg acgtggagct gaacgggctg ttcagcgccg
     3901 gttgtttcac ccggactgca ggctcttcgg aggctatcct aaaggaaaga gtcaagctgc
     3961 gacaccgaag aatctagtta ccaacctttg tttgtaacct gctgggcgcg aattatggac
     4021 gagctgtggg cgtcaagcgt ccgctgttac ttagtcgcct ttccatcagc caaggccggt
     4081 agttacgctg ttttctaatt ggcgccgaca agttttgcgt cacgcagcta ctccgcgtgg
     4141 acttcgcctt ctcgcgaagc ctggggacgc caccaagccg gatccctgcg accggtgtct
     4201 aagcgatctg caatcactat ggcgacccct ttactgaaac gaatcaggcc ggccttgatg
     4261 tgtctggtcg ttctcggaac acaggtagtg ccactcgcgc agacgcgtga gagtgataga
     4321 cctggtgagg ctgcttgcga gcacgaaatt aggaagcacg gggcacgacg actcggagaa
     4381 agcttcacgg ctcctatggt cacccgtcgt gtgcggcccg cctatccgac gatccccgac
     4441 gggacgactg gcggaggtgc ttggatcgga gaggtattga tcgacacagt cggcagagtc
     4501 ttgaatgtct ggacaatccg ggaggtaaag ctagagcccg ccttaccgtc gctgaacaaa
     4561 acgataaccg acgcggttct taggtggcag ttcgcgcccg ctgcggttga caaagttccc
     4621 gtaccgattt gtcaaaccgt tgcggtgaac gtcaaccttg acgcgctcag aagtgagcgc
     4681 tagcctagcg gcgggcgcgc gatgctgctg tgtttgatag tgaccttgct ggtacaaacg
     4741 ccgcgtttca cgtcgcgttt cacgtccccg agttgcgtac caactgaggg atggggtttc
     4801 gttatcgctg gacgcgatct agagaagagc ccacgctgga aaccaaccga cgataccccg
     4861 ccggtctcag cgcgagcagc ggaacgcgct gctcgtgcgt ttctgcgtcg cgtgccgtgc
     4921 aaggacgcgg acgcgtggga attgcagcag attgctctgc aaccgctgcc aggcgaacgc
     4981 gacgtgtggg tttacgtgat caaatatacg gagcccttgc ccctgcaaaa aggccccgcc
     5041 gtcggctcgt tcatcccgcg tatcgtagaa gtcccggtgc tgcttgacgg aagggtacca
     5101 accacctcgt ctggcccttg gccaccccga tgatcaggcc gccgcgggac ggcacacagc
     5161 gcggtggcct atccaatagg gaagagtcaa gccgcgatcc cggagaagtt ggttaccaac
     5221 ctttgttgta acctcccggg cgcctttggg ccgaaggcga aaacgcgccc gagcgcgccg
     5281 tagcagcgtc tgaattcaat gctgcggagc tgggccgcgt gccacactgc tttggagagc
     5341 tgtcgggagg agacgtgaca agcgcgaagt ccaccgcgtt gggtgtatcg gcttgtatgc
     5401 ttctggccat ctcgatcggg ttcgctcgcc agcaagatcg tgacctgagt ggcatccatg
     5461 gcataccggt cgagggacat gacgtcattg caggcgttga gatcgttggc accaaacagc
     5521 tcgatttgtc tcgatcggga tggcacaagc tcgacgagcg gcttcgggca aatggtgctc
     5581 aactgcgtcc ggggtggccc ctcgagaatc aaacgctgtg tcgcttcaag gaggtcctgc
     5641 gagacgtgat gagcgagaaa ggatttctcg acgtcgagat cacacacgac acgaggccga
     5701 cgtacggaaa tccccagcac ctgacgctca aattcaccat tgtcgaagga aagcgatctc
     5761 gcccggctgc accagtgctg tctccagcgg aacgctgtat gaggtgaagc ttgcgcgcgc
     5821 cgcggtagct tctgaaatca attgggaagc tggacaggaa tcagcgttca tggcacccta
     5881 ccacgcatga ctatcatcct cgtcttagcg attgccttgg ttgtgggagc cttggtccag
     5941 aaccgatggg cgttactgct ccctctcggg atcggtgccg ctacggcctt agccatggcg
     6001 gtgactggac atggtcttgg cgatactcca atccccttcc ttgtgatcgt gtgcacgctc
     6061 gtcatgctcg gaggccaagg cttgcgatca cggaaggtct cagccacgtc gtgagcgtgc
     6121 gcagccacgg atgggtgaaa ccaagaccaa gcccttgcca ccaaggttct tcaagacgct
     6181 cagctgacgc gtgccgccag ggtcatcgcg gacgttgggc cgctccaacc gagaatgcga
     6241 aacgactttc aatcgcggag cgtgcgtaga gagcttcgtc agcttcttcc ctccaacaaa
     6301 gatgacgtgg ccgccgttga ttcgctcatc gctctcgggt atccgtcaat cgaaccgatt
     6361 ctgcctgatg ttctgaagtg gatacgccaa ccggactggc cagtggctcc tccgctttcc
     6421 aagttcctcc tgagcctggg gccctcactc ttgccccata tccacaagat cttcagttct
     6481 cctcgggacg acgcctggaa ggcggagatt cttcgcacca tagtccaata ctggcccagg
     6541 gaacatgtcg cagaactgaa atcgtacctc gccatgctcg cgacccatgg aaacagttgg
     6601 ggagtcgact tgatcgctct acgccttctc gcccgtcatc aattagttga caggaactgg
     6661 attgccgact ggctggaatt caagagtaag tttcatctgg accaacttca ggagattgaa
     6721 agcattcgaa aggtcaattc gagccaggct acctagtgca atgggaggag cgggtagcgc
     6781 agatgtcgcg gatggtctgc ctcggagaac ggccgtccgg ctggtacaaa cattcaatgg
     6841 gacgcaacaa gagatcgtcc aaacgtggag cgagaacagg caaagcaaag aagaggcaag
     6901 ctcgagacga gcggcgcttc ttgaagcatt ccttgtatgg caatgtgccg ctgatccggc
     6961 acaccggcgc cggcgcgaac ggtcgggagt ccgaatggtg gcagtgcgat cctactttca
     7021 agccacgcct tccccggcat gccgttgaag gtgacgtcac gaagcaggtg ttctgcgcgg
     7081 gttgccacac acccaagtac ttctacgtcg atgaggtacg acattgcatc cagtgcggcg
     7141 atagttttac gttcagcggc tctgaacaga gattttggta cgaggtccgt cagttcaatt
     7201 ttcattcggt tccaattcgt tgcgctcggt gtcgacgaca gcgccgcagt gagcacgctc
     7261 ttcgtgaaca gattgccacc gctcggaacc tgatacaggc cgcgcccgat gatccaagcg
     7321 cgcacttggc cttggcacgc gcgcttgtgg aatatcacga acgcacgaat cagggtcgcc
     7381 ttcacgaggc aattgccgcc gccagacggg caggcaagct atggccggag gtccctgacg
     7441 cttgcttgtg ggaaggcatt gctcacgcga gagccggccg gcggaaacag gctcagacat
     7501 gtctgtccaa gttcctcaag cagcccaagg gaggctccgg gttcgaggtg aaggccaggc
     7561 ggtatctcag cgagacgaca gagggcgcat cggcctgatt tgcttttgca gccgacgcgc
     7621 tgcgcaagca cgcaccaagc aagtaatccc acacacgttc cactccgccc gtataatcac
     7681 gctcacattg cctgtcactg ccggcacgcg cctcggccca tatgtgatca cggcaccgct
     7741 cggcgccggc gggatgggcg aagtctataa agcaaccgat acccgcctga accgcaccgt
     7801 cgcaatcaag gtcctctcgg atcggctcgc cggcgacccg gagttacgcg cgcgattcga
     7861 gcgggaagcc cgttcggtgg ccctgctcga ccatcctcac at
//
LOCUS       DASUMG010000483         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_466296, whole genome shotgun sequence.
ACCESSION   DASUMG010000483 DASUMG010000000
VERSION     DASUMG010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466296"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VFX46_07980"
     CDS             complement(<1..801)
                     /locus_tag="VFX46_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX5600559.1"
                     /translation="MRSLLFVPGDSDRKIAKAMSSGADALILDLEDSVSLDRKEEARA
                     MTRDFIASAKGQEKRPRLYVRINALTTDLWSADLEGVMSAGPDGIILPKPRSGEDVHR
                     LSLALDHAEERAGLKQGSTRIVAIATEVPICVLNMASFVGSSSRLEGLTWGAEDLSAE
                     VGSTSTREPNGALTSPYRLARDLCLFTAVSAGVQPIDTVFIDFRDLDGLRAEAAAAAR
                     DGFTGKLAIHPDQVPIINAAFTPSQREIDHARDIVAMFSASPGAGVISL"
     gene            complement(805..1332)
                     /locus_tag="VFX46_07985"
     CDS             complement(805..1332)
                     /locus_tag="VFX46_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX5600560.1"
                     /translation="MSASCPNSSVIDQPIRTNRRRSAVRGLYYEEFEVGHVFEHAIGR
                     TVTEMDNTLFSCLTLNPQPLHIDHAFASQTEWGKPLVNSLFTLGLMIGISVSDTTLGT
                     TIANLGMTDVKFGKPVFHGDTIRVKTEVVSKRESRSRPDAGIVEFHHRAYNQHGDLVA
                     ECRRQAFMRKKPKDS"
     gene            complement(1298..2080)
                     /locus_tag="VFX46_07990"
     CDS             complement(1298..2080)
                     /locus_tag="VFX46_07990"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEX5600561.1"
                     /translation="MPNPKRKFEKIAFVASDHPEAQQALDRLTKQYGNVPSQTADAIV
                     ALGGDGLMLQTLHRHMNDRIPIYGMNRGSVGFLMNEYREDDLIARLEGAEISCIHPLV
                     MRARDIHGDLHEDLAINEVSLFRQTHQAAKLRILIDGKVRLEELICDGILVATPAGST
                     AYNLSAHGPILPINAPLLALTPISPFRPRRWRGALLPNDAHIQIDVLEPDKRPVSAVA
                     DHSETRSVVTVAVEEERSIDLFMMFDPGHSLDERILSEQFRY"
     gene            2140..2484
                     /gene="cutA"
                     /locus_tag="VFX46_07995"
     CDS             2140..2484
                     /gene="cutA"
                     /locus_tag="VFX46_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX5600562.1"
                     /translation="MQENDKAVLIYSTYPTTEAAEQTGTRLVDQGLAACVNIWPGMTS
                     IYIWQGERQRDSEVVMVIKTRASLADAVIAEAKSQHPYTNPAFLVLPVESGAPDFLRW
                     IVEQTRPSAEGR"
BASE COUNT          504 a          791 c          813 g          498 t
ORIGIN      
        1 gagggaaatg acgccagcac cgggagaggc agagaacatc gcgacgatgt cgcgcgcatg
       61 atcgatctcg cgctggctcg gtgtgaacgc ggcgttgata atggggactt gatccggatg
      121 gatcgcgagc ttgccggtga acccgtcgcg agccgcagca gccgcttccg cccgcaggcc
      181 atcgaggtcg cggaagtcga tgaatacggt gtcgatcggc tgcacgccgg ccgaaaccgc
      241 cgtgaacagg cagagatcgc gcgcgaggcg atacggggag gtcagcgcgc cgttcggctc
      301 gcgcgtggag gtcgagccaa cctcggccga caaatcctcc gctccccagg tgagcccttc
      361 caagcggctg ctcgatccga cgaagctcgc catgttcaag acgcagatcg gcacctcggt
      421 cgcgatggca acaatgcggg tggagccctg cttcaaaccg gccctttcct cggcgtgatc
      481 gagcgccagc gacaggcggt gcacatcctc gcccgagcgc ggcttcggca ggatgatgcc
      541 gtccggcccc gcagacatga cgccttcgag atcggcgctc caaagatcgg tcgtcagcgc
      601 attgatgcga acgtaaaggc gcggtcgctt ctcctgtcct ttggccgagg cgatgaaatc
      661 ccgcgtcatc gcccgcgcct cctccttgcg atcgagcgag accgagtcct ccaaatcgag
      721 aattagagca tcggcgccag aggacatggc tttcgcgatt ttgcgatcgc tgtcgccggg
      781 cacgaagaga agtgaacgca tggatcagct atccttgggt ttcttgcgca tgaaggcttg
      841 gcggcggcac tcggccacca gatcgccgtg ctggttgtag gcacggtgat gaaactcgac
      901 gatgccggcg tccgggcgcg agcgactctc gcgcttgctg accacctccg tcttcacccg
      961 gatcgtatcg ccgtggaaca cgggcttgcc gaatttcaca tcggtcatgc cgaggttggc
     1021 aatcgtcgtg ccgagggtcg tatccgaaac cgagatgccg atcatcaatc ccagcgtaaa
     1081 gaggctgttc acgagcggct tgccccactc ggtctgggag gcgaacgcat ggtcgatgtg
     1141 caacggctgg ggattgagcg tgaggcaaga gaagagggtg ttgtccatct ccgtcacggt
     1201 ccgaccgatg gcatgctcga acacgtgacc gacctcgaac tcctcgtagt agagaccccg
     1261 cacggcactt ctccttcgat ttgtacgaat cggttggtca ataacggaac tgttcggaca
     1321 ggatgcgctc atcgaggctg tggccaggat cgaacatcat gaatagatca attgaccgct
     1381 cttcctcgac agcgacggtg accacggagc gcgtctccga atgatcggcc acggcactga
     1441 ctggccgctt gtccggctcg agcacgtcga tttggatatg ggcgtcgttg ggcaacaatg
     1501 cgccccgcca ccttcgtggg cggaacgggc tgatgggtgt cagggccagc agcggcgcgt
     1561 tgatgggcag aatcggaccg tgcgccgaaa ggttgtaagc ggttgacccg gccggcgtcg
     1621 ccacaagtat cccatcgcag atgagctcct caagcctcac cttgccgtcg atgagaatgc
     1681 gcagcttggc cgcttgatgc gtttgccgga acagtgacac ctcgttgatc gcgaggtcct
     1741 cgtgaagatc gccatgaata tcgcgcgccc gcataacgag cggatggatg cagctgatct
     1801 cggcgccctc gagcctcgcg atcaggtcgt cttcgcggta ctcgttcatc aggaacccga
     1861 ccgacccgcg gttcattcca taaattggga tgcggtcgtt catatgcctg tgcagcgtct
     1921 gcagcatgag gccgtcgccg cccagcgcca ctatggcatc agccgtctga gacggcacgt
     1981 taccatattg tttggtcagc cggtccaaag cctgctgtgc ttccggatgg tcgctcgcga
     2041 cgaaggcgat cttctcgaat tttcgcttgg ggtttggcat gctgaccgca atcgtccagt
     2101 tctatccggc tccgcgctga ccgttggagg cttgctcaag tgcaggaaaa cgacaaggcc
     2161 gttctcattt attcgaccta cccgaccacg gaagccgccg agcagaccgg cacccggttg
     2221 gtagaccagg ggcttgccgc ctgtgtcaac atctggccgg gcatgacctc catttacatc
     2281 tggcaaggcg agcggcaacg cgacagcgag gtcgtcatgg tgatcaagac ccgtgcatcc
     2341 ctcgctgacg ctgtgatcgc cgaagcgaag agccaacatc cttacactaa tccggcgttc
     2401 ctggttctgc cggtggaatc cggcgcgccg gatttcctgc gctggatcgt ggagcagacg
     2461 cggccatcag cagagggccg ttgagggctg caccaaaacg agcgacaagg gtcacccaat
     2521 gcagcatctg cttaaccccc gcttggccga agaattgcgc agggtgccgg tcgtacctgt
     2581 gcttacaatc gatgacgcca agctgg
//
LOCUS       DASUMG010000484         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_65895, whole genome shotgun sequence.
ACCESSION   DASUMG010000484 DASUMG010000000
VERSION     DASUMG010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65895"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="VFX46_08000"
     CDS             <1..810
                     /locus_tag="VFX46_08000"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX5600563.1"
                     /translation="LHYKPKGPNDTVPLVEEGDAVAVAVALADKIDTLVGFWAIDEKP
                     TGSGDPYQLRRAALGIIRIVLENDLRLPLAKFIQKSLALQPSTVKPPAGLVEDLLSFF
                     ADRLKVHLREEGVRHDLIDAVFALPGQDDLALIVKRVEALGEFLETNDGANLLAGLKR
                     AASILAIEEKKDKTSYDGDYDIKLLQAPEELALAAAIESTKQDTSAAIAVENFKGAMR
                     ALAELRRPVDAFFEKVTVNVEDQKVRQNRLRLLSQIRAATMRVADFSKIAG"
     gene            826..1251
                     /locus_tag="VFX46_08005"
     CDS             826..1251
                     /locus_tag="VFX46_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX5600564.1"
                     /translation="MAQHLFVYGTLMSGAGHRMGARLQREARLVGLARIKGRLYDLGA
                     YPALVETGPADGDVHGEVFELIDPSRSLRWLDAYEGIVPGREAHSEYARVERTVRLED
                     GRELTAWVYIYQAPVHGRPVIESGLWLSRPRLNPLYRRG"
     gene            complement(1292..2071)
                     /locus_tag="VFX46_08010"
     CDS             complement(1292..2071)
                     /locus_tag="VFX46_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX5600565.1"
                     /translation="MASFDPKAAAAYVAEAHRMRAPYRNLPPEIAPPTVADAYAAQEA
                     LRELWTPIYGPVRGLKIATTTKVMQELMGIDHPCGGMIYERRIHQSPAQVRREDFVNL
                     MVEFELAVRLGTSLPPKGAPYTAAEVRAAVAEVMPAFELIEDRGADYKSTSALSLIAD
                     NAWNGGVVLGAPQKVPADLELNGLQGRLVVNEKDRGSGLTDNPMGALAWLANLAVERG
                     RPLDAGMIVITGSVLPTIPVAPGERLVFSLEGLGDAELSIS"
     gene            complement(2086..2694)
                     /locus_tag="VFX46_08015"
     CDS             complement(2086..2694)
                     /locus_tag="VFX46_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5600566.1"
                     /translation="MMKLLFSPASPYVRKVRITAAMKGVADRIENVPTDTLPLVNEDL
                     RRENPLSKIPVLITDDGMRIFDSHVICEYLDTLAPEPRLFPASGPERFRTLTLGALAD
                     GMLDAALLLVYEKRYRPEDKWVESWMQRQQAKIDTSLDWFEKSPPTWSDHPDYGHITL
                     ACALGYLDFRHGGQWRNGHPGLVAWLDKFAAAVPAFEETRPS"
     gene            complement(2799..3164)
                     /locus_tag="VFX46_08020"
     CDS             complement(2799..3164)
                     /locus_tag="VFX46_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600567.1"
                     /translation="MADQLLWFETLIRFSAGLVLLIMPLTAARVLGLPLPQALLWPRL
                     LGALLIGMAAATLLEGSISGSRGLGLGGLVLINLITAVVIIALLVLERGSQTKRGKLF
                     LRALAITLVGLGLIEIAVA"
     gene            complement(3197..3772)
                     /locus_tag="VFX46_08025"
     CDS             complement(3197..3772)
                     /locus_tag="VFX46_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600568.1"
                     /translation="MRGATVLVCRPQIILAASALVLAGCSSGGLTTASLTGGGNATEV
                     AAGEAASAGPPPSDPTSRTLQVAATSARASRCGFYFDPEKLKSSFLAAEMARGAGPEE
                     LQKIEREYNYVRSSVAEKIAEDSRYCDEARTKEIKRDLNRHLAGDFTPAPKKQEAQQG
                     LLAGLLDSDRSESKPFNADTFFEDLSRRNPR"
     gene            complement(3925..>4351)
                     /locus_tag="VFX46_08030"
     CDS             complement(3925..>4351)
                     /locus_tag="VFX46_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX5600569.1"
                     /translation="HHRVDPDPAPDIFDRRTAVLGFGSAIGQILLLDLVFSVDSIITA
                     VGMTTHVPIMVVAVIVAVLAMLLAANPLSDFINAHPTIVMLALAFLLMIGMVLIADGF
                     GVHVPRGYIYAAMAFSALVEGLNMLARRARRQRRGGGSH"
BASE COUNT          846 a         1316 c         1384 g          805 t
ORIGIN      
        1 ctgcattaca agcccaaggg gccgaacgat acggtgccgc tggtggagga gggcgacgcc
       61 gtcgccgttg ccgtggcgct ggcggacaag atcgatacgc tggtcggctt ctgggcgatt
      121 gatgagaagc ctaccggttc gggtgacccg tatcagctca ggcgcgcggc tctgggcatc
      181 atccggatcg tgctcgagaa cgatttgcgg ttgccgctcg caaaattcat ccagaaaagc
      241 ttggctctcc agccgagcac ggtgaagccc ccggccggat tggttgaaga ccttctttcc
      301 ttcttcgccg atcgtctcaa agttcacttg cgagaagagg gcgtgcggca cgatctcatc
      361 gatgccgtct tcgccttgcc cggccaggac gatctcgcac tgatcgtgaa gcgtgtcgaa
      421 gctctgggcg agttcttaga aaccaacgac ggggccaatc tgctcgccgg cttgaagcgt
      481 gccgccagca tcctcgctat cgaggaaaag aaggacaaga caagctacga tggcgattac
      541 gacatcaagc tgctccaggc accggaggag ctggcgctcg cagcagccat cgagagcacc
      601 aagcaggata cctcggctgc catcgcggtg gaaaacttca agggtgcgat gcgggcactg
      661 gccgagttgc gccgccctgt cgacgccttt ttcgagaagg tgacggtcaa cgtcgaggac
      721 cagaaagtgc gccagaaccg gttgcggctt ctctcgcaaa tccgcgcggc tacaatgaga
      781 gttgcggatt tctccaagat tgcggggtga tcggccgagc gcacgatggc gcagcatttg
      841 ttcgtctacg gcacgttgat gtcgggcgcg ggccaccgca tgggcgcgcg gctccagcgc
      901 gaggcgcggc tcgtgggact ggctcgcatc aaggggcggc tttacgacct tggtgcctac
      961 ccggcgctcg tggaaaccgg cccagccgat ggggatgtcc acggtgaagt atttgaactg
     1021 atcgatcctt cccgctcgct tcgctggctg gatgcctacg aaggcatcgt acccggccgc
     1081 gaggcgcaca gcgagtatgc ccgcgtagag cgtacggtga ggctcgagga tggccgggag
     1141 ctgacggcct gggtgtacat ctaccaagcg ccggtgcacg gcaggccagt cattgaaagc
     1201 gggctctggc tatcgcgccc gcgccttaat ccactctatc ggcgtggctg agtgatttcg
     1261 gccgctcgcg cgaaatgctc aaagccagcc attatgaaat ggatagctct gcgtctccga
     1321 ggccttcgag ggagaaaaca agcctctccc ccggagcgac gggaatagtg ggcagaacgc
     1381 tgccggtgat aacgatcatg ccggcgtcga gcgggcggcc gcgttcgaca gccagattgg
     1441 ctagccaagc cagagcgccc atcggattat cggtaaggcc tgagcctcga tccttctcgt
     1501 tgaccacgag gcggccttga agtccgttga gctcgaggtc cgccggcacc ttttgtggcg
     1561 cgccgaggac cacgccgcca ttccaggcat tgtccgcgat cagcgagagc gcgcttgtgc
     1621 tcttgtagtc ggcgccacgg tcctcaatca gctcgaaggc gggcatgacc tcggccacgg
     1681 ctgcccggac ttcggcggct gtgtaaggtg caccttttgg cgggaggctg gtgccaagtc
     1741 tcacggccag ctcgaactcg accatgagat tgacgaagtc ctcccgccgc acctgcgccg
     1801 gcgactgatg gatgcgccgc tcgtagatca tgccgccgca tgggtgatcg atgcccatca
     1861 gctcctgcat gaccttggtg gtcgtcgcga tcttgagccc gcggacaggg ccgtaaatcg
     1921 gcgtccatag ctcgcggagc gcttcctggg cggcgtaggc gtcagcgacg gtcgggggcg
     1981 cgatttcagg cggcaaattc ctgtaaggcg ctcgcatacg gtgcgcctcg gccacgtacg
     2041 ccgcagccgc ctttgggtcg aacgaggcca tgcgctgccc cttcctcagc ttggccgcgt
     2101 ctcctcgaag gctgggacgg ctgctgcaaa cttatcgagc caggcgacca gccctggatg
     2161 tccattgcgc cactgcccgc catgacgaaa gtcgaggtag ccgagcgcgc aggcaagagt
     2221 gatgtggccg taatccggat ggtcagacca cgtcggtggc gacttttcga accagtcgag
     2281 ggacgtgtcg atcttggcct gctggcgttg catccagctc tcgacccact tgtcctccgg
     2341 ccgatagcgc ttctcgtaga cgagcagcaa tgccgcatcg agcatgccgt ctgcgagcgc
     2401 gccaagggtc agcgttcgga accgttctgg gccagaagcg gggaacagcc gcggttcggg
     2461 cgcgagcgtg tcgaggtatt cgcagatcac gtggctgtcg aaaatccgca tgccgtcgtc
     2521 ggtgatcaga actggaattt tggaaagcgg attttcccgg cgcaaatcct cgttgaccaa
     2581 tggcagggtg tcggtcggca cattctcaat gcgatcagcg acgcccttca tcgcggcagt
     2641 gatcctgact ttgcggacgt aaggcgaggc aggagagaac aggagcttca tcatggcctt
     2701 gaaccgcggt gcttgagttg gacccaacgt gcaagctgca tcgtgccggg gatgcaagcg
     2761 gtcggcaagc tcggatcgca ctctatttca ttaaaacgtc aggcaaccgc gatctcaatg
     2821 agaccaaggc caacgagcgt tatggccagg gcccgcagaa acagcttgcc gcgtttcgtc
     2881 tgactgccgc gctcgagcac gagcaaggcg ataatgacta ctgctgtgat gagattgatg
     2941 agcacgagcc cgccaagccc cagcccgcgg gagcctgaga tgctgccttc gagcagcgtc
     3001 gcggcggcca tgccaatgag aagagcaccg agcaaccgcg gccaaagaag cgcctgtggc
     3061 aacggcaatc caagcaccct tgccgccgtc aacggcatga tcagcaacac taggccagcc
     3121 gaaaacctga tcagcgtctc gaaccagaga agttgatcgg ccatggtctc ggccgcttgt
     3181 ggctaatcat cccacttcag cgcggattcc tccgactgag atcctcgaag aaggtgtcgg
     3241 cgttgaaagg cttgctctcg ctgcgatcac tgtcgagcag cccggccagc aggccctgct
     3301 gcgcctcctg tttcttgggc gccggcgtga agtcgccagc gagatggcgg ttgaggtcgc
     3361 gcttgatctc cttggttctg gcctcgtcgc agtatcggct gtcctcggcg attttttcag
     3421 cgacggacga gcggacgtaa ttgtattcgc gctcgatctt ctgaagctcc tccgggccgg
     3481 cgccccgcgc catctcggca gccaggaagc tcgacttgag cttttcgggg tcgaaataga
     3541 agccgcagcg gctagcgcga gcggaagtgg cggcgacttg aagcgtgcgg gaagtcggat
     3601 cgcttggcgg cgggccggcc gatgccgcct ctccggccgc tacttccgtt gcattaccgc
     3661 cgcccgtcag tgatgcggtc gtaaggcccc ccgagctgca tccggcgagc acaagcgcgg
     3721 acgcggctaa gatgatctgc ggcctgcaaa cgagcacggt cgcccccctc atccgtgttt
     3781 ctcccaagaa attgggaatc ttggaacatt tcgtgcacag tgcgcttcgt ggacgtacac
     3841 gtcaagcaca gaacggtcac gctttgcggc ggaacgtagt catatcctca caattcgccc
     3901 caaatgtgcc gggcggcacg ccgtctaatg agagccgccg ccgcggcgtt gccgccgtgc
     3961 tcggcgcgca agcatgttga gaccctcgac cagagccgaa aaggccattg cagcatagat
     4021 gtagcctctc ggtacgtgca ccccgaaccc atccgcgatc agcaccatgc cgatcattaa
     4081 taggaaggcg agcgcaagca tcacgatcgt cgggtgagcg ttaatgaaat cagagagcgg
     4141 atttgccgcg agcaacatgg caagcacggc gacgatgaca gccacgacca taatgggcac
     4201 gtgcgtcgtc atgccgaccg cggtgatgat gctgtcgacc gagaaaacga ggtctagcag
     4261 aagaatctgc ccaatggccg atccaaaccc gagcacagcc gttctgcggt cgaaaatgtc
     4321 gggcgccgga tcggggtcga cccgatgatg g
//
LOCUS       DASUMG010000485         2363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_788291, whole genome shotgun sequence.
ACCESSION   DASUMG010000485 DASUMG010000000
VERSION     DASUMG010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788291"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VFX46_08035"
     CDS             complement(<1..610)
                     /locus_tag="VFX46_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX5600570.1"
                     /translation="MIGLVRATVICATLALGLTAAGAADKAFKRDELADSAVKLEAQI
                     KSEAGPVAKSSATLRNDADAAFKRADFRVGLQILGQIAATTPEDSANWLKLAKTIFQI
                     RSASSSEQTFLYERASTAAYIAYQRAGNQAEEADALAVLGRAMSERKLWRPALDALRM
                     SLDMREVAEVRGSYEKLRDQHGFRLLDYTVDSDGASPRACFQF"
     gene            939..1015
                     /locus_tag="VFX46_08040"
     tRNA            939..1015
                     /locus_tag="VFX46_08040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:973..975,aa:Arg,seq:tct)
     gene            1220..1465
                     /locus_tag="VFX46_08045"
     CDS             1220..1465
                     /locus_tag="VFX46_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEX5600571.1"
                     /translation="MITSIAILAVSAMPALADRQVTEDERAKLVAAVQAEGCSGGKFE
                     MDEDDQQFEVDDAVCADGKKYDLKFDMQMRLKRKNLD"
     gene            1654..1911
                     /locus_tag="VFX46_08050"
     CDS             1654..1911
                     /locus_tag="VFX46_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600572.1"
                     /translation="MPKKGITGHDEWVVTEALATALVALEQLEPTQQSRQQMDDIRKL
                     LAANCQPGTLNLHLAQAKCRLNPHGDRADIYREYGFEDWEV"
     gene            2049..2333
                     /locus_tag="VFX46_08055"
     CDS             2049..2333
                     /locus_tag="VFX46_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3551 domain-containing protein"
                     /protein_id="HEX5600573.1"
                     /translation="MKLPHAFAAAMSLTILAATIALPAPARAQTYDPSYPVCLQVYQG
                     IVDFYFECHYRTMAQCMASASGRSAQCVVNPYYGGPKAKRSKRQQRSRQY"
BASE COUNT          480 a          708 c          741 g          434 t
ORIGIN      
        1 agaactggaa acaggcacgc ggcgaggcgc catcggagtc cacagtatag tccagcaggc
       61 ggaacccatg ctggtcgcgc aatttctcgt aactgccgcg gacctcggcg acctcgcgca
      121 tgtcgagcga catccgcaac gcatccagcg ccggacgcca cagcttgcgc tcggacatcg
      181 cccggccaag caccgccagc gcatcggcct cctcggcctg gttgccggcg cgctgataag
      241 cgatataggc cgcagtcgag gcgcgctcgt acaggaaggt ctgttcgctg gaactggcgg
      301 agcggatctg gaagatggtt ttggcgagct tcagccagtt ggcgctgtct tcgggggtcg
      361 tggccgcgat ctgccccagg atttgcaagc ccacacggaa gtcggcgcgc ttgaaagcgg
      421 cgtcggcgtc gttgcgcagc gtcgcggagg atttggcgac cggccccgcc tcgctcttga
      481 tctgggcctc cagcttgacg gccgaatcgg ccaactcgtc gcgcttgaac gccttgtccg
      541 cggcccctgc cgccgtcagg ccaagcgcca gagtggcgca aatcactgtg gcgcgaacca
      601 aaccgatcat aatgggctcc ttgccgcggg catcagccgc gtaacgtcga aaagggcgcg
      661 aaaaggtgac ggactgatgg cagtggattc ggcttgcgaa agggtcgcat gcatggcgct
      721 ggcagtccaa acggtcccgg ccgcaccgcc cggcgggggt gccggcggct ggctttgtgt
      781 caggggcgga ccacatttaa ggccagccgt ctgccttggt cgcgaacttg aggaaatcgg
      841 ttcgaccgtc ttggcgggaa agcggatttt tcatattggc agtgctagat aagcaaggtt
      901 tatacaaggc ggatacccag ggcgggtacc gcccggacgg tcccatagct caactggata
      961 gagtaacgga tttctactcc gtgggttgca ggttcgaatc ctgctgggat cgccaccttc
     1021 tgtgccgatg aaacagcacc atcggacctg aaagccgcgc agatctacca gctggttacc
     1081 tgatttgcgg aacgtttgcc ggtcgccggc attcgaattg tgtgtgatgg ttcccacgca
     1141 atgccctgac gcggcgagaa aaccggctgt catcgcaaag cgctcaccgc acgcccaccg
     1201 gaggacctag ctgatgcgaa tgatcacatc gatagcaata ctcgccgtat ccgccatgcc
     1261 tgcgctcgcc gatcgccagg ttaccgagga cgaacgcgca aagctcgttg cggcggttca
     1321 ggccgaggga tgttccggcg gcaagttcga gatggacgaa gacgatcagc agttcgaggt
     1381 cgacgatgcg gtatgtgcgg atggaaagaa atatgacctc aagttcgata tgcagatgcg
     1441 gttgaagcgc aaaaatctgg attgagttat gcggccaccc agatttttat gccgtgggtc
     1501 gcggcttcga atcctgttgg catcgccaac aaaatagcgc tgacagccgc gcgccgttcg
     1561 caatttcgac gtcattctcg actcgacttg gacgtaagcc tacgatgccg gccggaattt
     1621 gctccgttgc cctgacaaag gatgcgggtc gccatgccga aaaagggaat caccggccac
     1681 gacgaatggg tcgtcacgga ggcgctggca actgcactgg tcgcactgga gcagctcgag
     1741 ccgacccagc aatcccgcca gcagatggac gatatccgga aattgctggc agccaactgt
     1801 cagcccggaa ccctcaatct ccacctcgcc caggccaagt gccggctgaa tccgcacggc
     1861 gatcgagccg acatctatcg agagtacggc tttgaggact gggaagtctg aaatgcggga
     1921 ctcgtggtaa gcccagaccg gatgagcccg gatgagcgta gcgatatccg agaagcgctg
     1981 tgtcctgcat cgcgctgcgc tcaagcgggc tacttgatag aagcgcggaa gtccgggagg
     2041 atatcaggat gaagctgcca cacgcatttg ccgccgcgat gtctttgaca attttggccg
     2101 ccacgatcgc cctgccggcg cccgcccgcg cccagaccta cgatccgagc taccccgttt
     2161 gcctgcaggt ctaccagggc atcgtggact tctacttcga gtgccactat cgaacgatgg
     2221 cgcagtgcat ggcgtcggct tcaggccgtt ctgcccagtg cgtggtcaat ccatattacg
     2281 gcgggccgaa agccaagcgt agcaagcgtc agcagcggtc ccggcaatat tgagcacggc
     2341 ctgaccggcc gccggaggcc tcg
//
LOCUS       DASUMG010000486         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_868230, whole genome shotgun sequence.
ACCESSION   DASUMG010000486 DASUMG010000000
VERSION     DASUMG010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868230"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VFX46_08060"
     CDS             complement(<1..480)
                     /locus_tag="VFX46_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEX5600574.1"
                     /translation="MPATRTPAKFYAPLAIGAPQPLRQLPVRLERLLHFIPPHNPKIR
                     ARLPETIATVDVLVGNLEDAIPADEKEAARRGFIELAQQHDFGKTGLWVRINALNSPW
                     VLDDLLEIVPAVGSKLDVIMLPKVEGPWDIHYLDQLLAQLEAKHRIEKPILIHALLET
                     "
     gene            complement(550..1500)
                     /locus_tag="VFX46_08065"
     CDS             complement(550..1500)
                     /locus_tag="VFX46_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX5600575.1"
                     /translation="MPKVYKKRRSRFRLPPALRDLQFRAEYLLLRLVAGLVRAMPLSV
                     AVAVSAKAWRWLAPVISRVRHKRALANLAIAFPEKSEAERLEIALAHWENLGRVMAET
                     MQIDRVIRDPSRIEIENLALFSRYKDKLGPAIGVSLHMGNWELAIWPLVVAGANPAAV
                     YRSVNNPYVDRYLRAQRKDLYPGGLFGRGRVEGDHDESQRTARIITDYVRRGGRLGLV
                     CDLHDRTGIPVPFFGKPARTQAIAAMIARRVGARIWLARCLRIGKESRFRIQIKELKV
                     PRTSNPADDIRAIMTEMQAQFELWVREAPEQWMWSNRRWS"
     gene            1650..2102
                     /gene="arfB"
                     /locus_tag="VFX46_08070"
     CDS             1650..2102
                     /gene="arfB"
                     /locus_tag="VFX46_08070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX5600576.1"
                     /translation="MTGHPREGITVIRISDTLSIPETEIEERFIRASGPGGQNVNKVS
                     TAVELRFNVAASESLPPAVRVRLRRLAGRRMTKDGVLVLRAERHRTQELNRAEVRRRL
                     AELIRRASVEPKRRIPTRPSQAAKERRLELKARRSEIKRLRSMKPELE"
     gene            2160..3395
                     /locus_tag="VFX46_08075"
     CDS             2160..3395
                     /locus_tag="VFX46_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5600577.1"
                     /translation="MSNETISTTCAVAGGGPAGLMLGFLLARAGVPVVVLEKHADFLR
                     DFRGDTIHPSTLELMAELGLDEAVLRLPHQKVPRIGGTFGGETLLFAEFSRLPVRHPF
                     IAMMPQWDFLNFLAERGRRYPNFSLFMEANVEDLLHANGRVAGLMARTSTGQLEVRAD
                     LVVAADGRHSVLRDRAGLVPDDLGAPMDVLWFRLSRAPGDSSESIGHFEPGRLVVQIN
                     RGNYWQCAMVIPKGAAHAVRERGLDTFRAQVTDVLPWARQRISEDLKTWDDVKLLSVR
                     VDRLWQWYKPGLLFIGDAAHAMSPIGGVGINLAIQDAVAAANILWQPLRDKQLETRHL
                     LAVQRRREWPARVTQWLQIAIQNRVIRPTLAAERPLRPPLLLRLAARFPVLRRLTARV
                     VGIGIRPEHIESPELPPPK"
     gene            complement(3408..>3769)
                     /locus_tag="VFX46_08080"
     CDS             complement(3408..>3769)
                     /locus_tag="VFX46_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX5600578.1"
                     /translation="VKLQHKAAAVGFDWPSLEPVMAKLKEELGELESAIAADKKTKVQ
                     EEFGDLLFVLANVARHLEIDPESALRAANEKFVRRFRYIEEALAARGKKPTESSLEEM
                     DALWNEAKEAESRASSQ"
BASE COUNT          667 a         1184 c         1206 g          712 t
ORIGIN      
        1 cgtttccaaa agggcgtgga tgaggatcgg tttctcgatg cgatgcttgg cttcgagctg
       61 cgcgagcagc tggtcgaggt aatgaatgtc ccaagggcct tcaaccttgg gaagcatgat
      121 cacatcgagc ttggagccga cggccggcac gatctccaga agatcgtcga gcacccacgg
      181 cgagtttaga gcattgatgc gtacccacag gcccgtcttg ccgaagtcgt gctgctgcgc
      241 caactcgatg aagccgcggc gcgccgcctc tttctcgtcg gctggtatcg catcctccaa
      301 gttgccgacg agcacgtcta cggtggcaat cgtctccggc aaacgggcgc ggatctttgg
      361 gttgtgcggg gggatgaaat ggagcaaccg ctccagcctg acgggaagct ggcgcagcgg
      421 ttgcggtgcg ccgatggcga gcggtgcata gaacttggct ggggtacgcg tagctggcat
      481 tgaactcctc aattcgagat ttcttgttgt gaacgccgga cgactagcat cagtctcgca
      541 accggccaat cagctccagc gcctgttcga ccacatccac tgctccggcg cttctcggac
      601 ccacagctcg aactgggctt gcatttcggt catgatggcc ctgatgtcgt cggccgggtt
      661 ggaagtgcgc ggcaccttca actccttgat ttgaatgcgg aagcggctct ccttgccgat
      721 gcggaggcag cgcgcaagcc agatgcgcgc gcccacccgt cgagcgatca ttgcggcgat
      781 tgcctgtgtt ctcgccggct tgccgaagaa cggcactggg atgcccgtcc ggtcgtgaag
      841 atcacaaacg agacctaggc gcccgccacg gcgtacataa tcggtgatga tccttgccgt
      901 ccgctggctc tcgtcgtggt cgccctcaac tctgcctctt ccgaacagcc cgccggggta
      961 gaggtccttc cgttgtgccc gcaaataccg gtcgacgtat gggttgttga cggagcggta
     1021 gacggcggcc gggttggcgc ccgcaacgac cagcggccag atggcaagct cccagttgcc
     1081 catgtgcagc gagacgccga tcgccggtcc gagcttgtcc ttgtagcggc tgaacagcgc
     1141 gagattttcg atctcgatgc gggagggatc gcggatcacg cggtcgatct gcattgtttc
     1201 agccatgacc cgcccgaggt tctcccagtg ggcgagggcg atctccaggc gctccgcctc
     1261 gctcttctcc ggaaaggcga tggcgagatt tgccagagcc cgcttgtgcc ggacgcgcga
     1321 tatgacaggc gccaaccagc gccacgcctt cgcagacacg gcaacggcaa cgctcagcgg
     1381 catggcgcgg accagtccgg cgacaagacg gagcaacagg tactcggcac ggaactggag
     1441 gtcacgcaac gccggcggca gccggaatct ggatcgcctc ttcttgtaga ccttcggcac
     1501 cagtcgtgag cctttcgttg tggacgcagg ttgatgccaa ctgagagagc accacgcaag
     1561 ggtcagcagg ccgttatcct ggtgccaatg gcgccggaat tttgatgggt ggggttcaag
     1621 cggctgatga gttcctttct cgtttgtaga tgacgggcca cccccgcgag ggaataactg
     1681 tgattcgcat ttccgacacg ctcagcatcc ccgagacgga gatcgaggag cgcttcattc
     1741 gcgcttcggg gccgggcggg caaaacgtca acaaggtctc gaccgctgtg gagctgcgct
     1801 tcaacgtggc agcgagcgaa agcctgccgc ctgccgtgcg cgttcgcctg cggcgccttg
     1861 ccggccggcg gatgaccaag gacggcgttc tcgtccttcg cgccgaacgt caccgcacgc
     1921 aggagctcaa ccgggcagag gttcgtcgcc gactggcaga gctgatccga cgggcttccg
     1981 ttgagccgaa gcggcgcatt cccacgcgac caagccaggc agccaaggaa cgccggctcg
     2041 aactcaaggc ccgtcgcagc gaaatcaaac ggctgcggag catgaagcct gagctcgaat
     2101 agctcgaaga gtgtgtgagc gcgtttctcc acccagaagg gcatatccga cgtcaggcta
     2161 tgagcaacga aaccatctcg accacctgtg ccgtcgctgg cggtggcccg gcaggtttga
     2221 tgctcggttt tctcctcgcc cgagcgggcg ttccggtggt ggttctcgag aaacacgcgg
     2281 atttcctccg cgacttccgc ggcgacacca tccacccctc gacgctggag ctgatggctg
     2341 aactcggcct cgacgaggct gtgctccggc tgccgcacca gaaggtgccg cgcattggtg
     2401 ggacgttcgg tggcgagacg ctcctgttcg ccgagttttc gcgccttccg gtccggcatc
     2461 ccttcatcgc catgatgccg caatgggact tcctcaactt cctggcggag cgggggcgcc
     2521 gctatcccaa cttctccctg tttatggagg cgaacgtcga agacctgctg cacgcaaacg
     2581 ggcgcgtagc gggcctgatg gcacgcacgt cgactggaca gctggaggtg agggccgatc
     2641 ttgtcgtcgc cgcggacggg cgtcactcgg tgcttcgcga tcgggccggt ctagtgcccg
     2701 acgacctcgg cgctcccatg gacgtcctgt ggtttcgcct ctcgcgcgcg ccgggagatt
     2761 cttcggaaag cattggtcac ttcgagcccg gccgcctcgt ggtccagatc aaccggggca
     2821 actactggca atgcgccatg gtcattccga agggagctgc gcacgctgtg cgggaacgcg
     2881 gcctcgatac ctttcgggcc caggtgacgg acgttctgcc gtgggcaagg caacgcatca
     2941 gcgaggacct caagacctgg gacgatgtga aattgctcag cgtccgcgtc gacaggttat
     3001 ggcagtggta caagccggga ttgctgttca tcggtgatgc tgctcacgcc atgtcgccga
     3061 tcggcggggt gggcatcaac ctcgccattc aagatgccgt cgcggcggcc aacatcctct
     3121 ggcagccatt gagggataag caactcgaaa cccgccatct cttggccgtg cagcgccgcc
     3181 gcgaatggcc ggccagggtg acacagtggc tgcagatcgc gatccagaac cgcgtgatcc
     3241 ggccgacctt ggcggctgaa cggccattac ggccgccgct gctcctgcga ctggcggcga
     3301 gatttccggt tctgcgccgg ctgacggcgc gagtcgtcgg gatcggcatc cggccggagc
     3361 atattgagtc gccagagctg ccgccgccga agtgacacgt catacggtca ctgcgaggaa
     3421 gcacgggatt ccgcctcctt ggcctcattc caaagcgcgt ccatctcctc aaggcttgac
     3481 tcggtgggct tcttcccgcg agccgcgaga gcctcctcga tgtagcggaa gcggcgcacg
     3541 aacttctcgt tggcggcgcg cagcgccgac tccggatcga tctcgaggtg gcgggcgaca
     3601 ttggcgagca cgaacagcag gtcgccaaac tcctcctgaa ccttcgtctt cttgtccgca
     3661 gcgatggctg actcaagctc ccccaactct tccttcagct tggccataac gggttcaaga
     3721 gacggccagt cgaagccgac ggcggccgcc ttatgctgca gcttgacgg
//
LOCUS       DASUMG010000487         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1190427, whole genome shotgun sequence.
ACCESSION   DASUMG010000487 DASUMG010000000
VERSION     DASUMG010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190427"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFX46_08085"
     CDS             <1..255
                     /locus_tag="VFX46_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5600579.1"
                     /translation="LDTFSELAYGGTGQITDWGLAADVAKSTPILLAGGLTPENVTEA
                     IRIVRPYGVDVSSGVESAPGKKDHAKMRAFVDAVRVVSSS"
     gene            278..1489
                     /gene="trpB"
                     /locus_tag="VFX46_08090"
     CDS             278..1489
                     /gene="trpB"
                     /locus_tag="VFX46_08090"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX5600580.1"
                     /translation="MTNRKLTASVPDRQGRFGVYGGRYVPETLMPALLELEREYAQAK
                     RDRRFQRELAYYLKHYVGRPTPLYFAKRLTNRLGGAKIYLKREDLCHTGAHKINNAIG
                     QGLLARRMHKPRIIAETGAGQHGVATATVAAMFGLQCEIYMGTEDMQRQTLNVFRMRL
                     LGSTVTAVDAGSRTLKDAISEAMRDWTTNIETTHYILGSVLGAHPYPMMIRDFQSIIG
                     KEARKQVLAVEGRLPDCLIACVGGGSNAMGLFHAFLSDKKVKMIGVEAGGRGVGSGKH
                     AARFSGGSLGVLQGTMTYLLQNEDGQIDLTHSVSAGLDYAAVGPEHSYLREIGRTTYT
                     SATDEEALAAFDLLAREEGIVPALESAHAIAHVVKMAPTMKRQQVLLVNLSGRGDKDV
                     QQVARMRGISL"
     gene            1504..>2178
                     /gene="trpA"
                     /locus_tag="VFX46_08095"
     CDS             1504..>2178
                     /gene="trpA"
                     /locus_tag="VFX46_08095"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX5600581.1"
                     /translation="MTHRRRQTQGRTTISIGNSLRIAEDPSASGSRLDRTFRRLKARQ
                     EKALITYLMAGDPSLAETEQLVLELEKAGADIIELGVPFSDPIADGPVIQQAAERALR
                     SGTTLRGILSMVGRVRTQSEIPLVLMAYYNSIHAFGPDRFCREAVAAGVDGLIVPDMP
                     PDEAGPLKGPAAASGLQLIFLLAPTSTAERRTFVARQSHGFVYYVSLTGITGAKLGRV
                     SEIGRNV"
BASE COUNT          443 a          641 c          686 g          408 t
ORIGIN      
        1 ctcgacacgt tttcggagct ggcctacggg gggacgggtc agattaccga ttggggtctc
       61 gctgcggatg tcgccaaaag cacgcctatc ctactcgcag gcggtctcac tcccgagaat
      121 gtgaccgagg cgattcggat cgtccggccc tacggcgtcg acgtgagcag cggagtggaa
      181 tcggctccgg gcaagaaaga ccatgcaaaa atgcgcgcgt ttgtcgacgc cgtcagggtt
      241 gtctcttctt cgtgagcctg tttatactgc cagcaccatg acgaatcgca aactcaccgc
      301 gagtgtgcct gaccgccagg gccgtttcgg ggtttatggg ggtcggtatg tccccgagac
      361 cttgatgccc gccctcctgg aattggagcg cgaatacgcg caggcgaaaa gagaccggcg
      421 ctttcaaaga gaactcgcct attatctcaa gcactatgtc ggacggccaa cacctctgta
      481 ttttgccaag cggctcacga accgcctcgg cggcgccaag atctatctca agcgggagga
      541 cctctgtcat acgggcgctc acaagatcaa caatgccatt gggcagggac tcctggccag
      601 gcgcatgcac aagcctcgca tcatcgccga aaccggcgcc ggtcaacacg gcgtcgcgac
      661 cgccaccgtc gccgccatgt tcggattgca atgtgaaatc tacatgggca ctgaggacat
      721 gcaacgccag acgttgaacg tgtttcgcat gcggctcctc gggtccacgg tcaccgctgt
      781 ggacgccggc agccggacgc tcaaggacgc gatcagcgag gcgatgcgcg actggactac
      841 gaacatcgag acgactcatt acattctcgg ctccgtgctt ggcgcgcacc catatcccat
      901 gatgattcga gattttcaat cgattatcgg aaaagaagcc cgcaagcagg tgctcgcggt
      961 cgaggggcgg ttgccggact gcctcatcgc ctgtgtcggc ggcggcagca atgccatggg
     1021 actatttcac gcctttttga gcgataagaa ggtcaagatg atcggcgtcg aggccggagg
     1081 gcgtggggtg ggcagcggca agcatgcagc ccggttttca ggcggctcgc tgggcgtatt
     1141 gcaaggaacc atgacgtatc tgttgcagaa cgaggacggc cagatcgacc tgacccattc
     1201 ggtatccgcc ggtctcgact atgccgccgt cggccccgag cacagctatc tccgggagat
     1261 cgggaggacc acctacacgt cggcaaccga cgaagaggcg ctggcggcat tcgatcttct
     1321 cgcgcgggaa gaaggcatcg tgccggcgct cgaaagcgcg cacgcgattg cccacgtggt
     1381 caaaatggcg ccgacgatga agcggcagca ggtgctcctt gtcaatctct cggggcgggg
     1441 agacaaagac gtccagcagg tggctcgcat gaggggaatc agcttataag cgacgggtga
     1501 tgggtgacgc atcgccggag acagacacaa ggccgtacga cgatctccat cggcaactcc
     1561 ttgcgaattg ccgaggatcc gtccgcgagt ggtagccggc tcgaccggac attccgccga
     1621 ctcaaggccc ggcaggaaaa agcgctcatt acctatctca tggcgggcga tccaagcttg
     1681 gcggaaaccg agcaactcgt cttggagttg gaaaaggcgg gggccgatat tattgagttg
     1741 ggcgtgccat tttccgatcc gatcgccgat ggaccggtga ttcaacaggc cgccgaacgc
     1801 gcgctgcgaa gcggcacgac gctgcgcggc atcctttcga tggtaggtcg cgtacgaaca
     1861 cagtccgaga ttccccttgt cctcatggcc tattacaaca gcattcatgc cttcgggcct
     1921 gaccggttct gccgggaggc cgtggcggcc ggtgtggatg ggctgatcgt tcccgacatg
     1981 ccaccggacg aagccgggcc gctgaaggga cctgcggcgg cctctggact tcagttgatt
     2041 ttcctgctgg ctccaaccag cacggccgaa cggcggacat tcgtcgcccg gcagtcacac
     2101 gggttcgtct actacgtctc gttgacgggc attacagggg cgaagctcgg aagggtttct
     2161 gaaatcggtc ggaacgtt
//
LOCUS       DASUMG010000488         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_146892, whole genome shotgun sequence.
ACCESSION   DASUMG010000488 DASUMG010000000
VERSION     DASUMG010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146892"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VFX46_08100"
     CDS             complement(<1..457)
                     /locus_tag="VFX46_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012756561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX5600582.1"
                     /translation="MFTLYYAPNTCALATHIALLDAGAEYELKRIDFSKSQQQSPEYL
                     AINPKARVPAMVTPRGILTETPALLAFVAQSFPEAKLAPLDDPFAFAQLQEFNSYLCS
                     TVHVAHAHRVRGYRWADDPAAIEAMKKKVPQSVGACFELIEREMLKGPWV"
     gene            complement(800..1738)
                     /gene="tsf"
                     /locus_tag="VFX46_08105"
     CDS             complement(800..1738)
                     /gene="tsf"
                     /locus_tag="VFX46_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX5600583.1"
                     /translation="MTQISAAMVKELRDKTGAGMMDCKQALIENNGDIEASIDWLRKK
                     GLSKAAKKAGRVAAEGLIGVAVEGRKGALVEVNSETDFVARNAQFQEMVRDISKLALQ
                     ADGDLSRLLATPFPGKAISVEDHVKEMIATIGENMGVRRTATLEAGADGVLVDYVHNR
                     VAEGLGKIGVLVALESTGDKAKLAEFGRQIAMHVAAAAPLAISAEDLDPAVIERERKI
                     YEEQAAASGKPQDIVAKMAEGRLRKEFFQQVLLMQQIFVIDGKSTVEQAVKEAEGTVG
                     APVKITGFVRYALGEGIERKEEDFAAEVAAAVSGTR"
     gene            complement(1842..2825)
                     /locus_tag="VFX46_08110"
     CDS             complement(1842..2825)
                     /locus_tag="VFX46_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX5600584.1"
                     /translation="MLPTFTMRQLLEAGVHFGHQAHRWNPKMRQYIFGVRNKIHIIDL
                     TQTVPLLHQALVKISDTVAEGGRVLFVGTKRQAADAIAEAAKRSAQYYINHRWLGGTL
                     TNWKTISQSIRRLRQLDEILADPVGRTKKEVLNLTRERDKLNQALGGIKEMGGTPDLL
                     FVIDTNKEAIAVQEAKKLGIPIVAVVDTNCDPDGIDFPIPGNDDAGRAITLYCDLAAR
                     AAIDGIQRAQAAAGIDIGEAVTPPEEALPFVFKGIEAPRGEPDDLKRINGISAKLEQR
                     LNDLGVFHFWQIADLDTESMQALDRQLKLNGQIEKEDWIGQAKKLVEAAAA"
     gene            3049..>4053
                     /locus_tag="VFX46_08115"
     CDS             3049..>4053
                     /locus_tag="VFX46_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX5600585.1"
                     /translation="MVASEHIPAASPHRRKTAPQRGSLALGLAMLGAMTLRRRFDRGK
                     EAEVGQVSSPRPKQSLDRGIAPGRGREAQSPSEIPAAGWKDVLWRVYERFQSDRVMLI
                     AAGVTYYGLLALFPAIAALVSLYGLFADPDTIKQQLSNLQGVLPEDAIQIIGDQITHI
                     QAQGGGRLGIYFAIGLIVSLWSANAGVKSMFDALNAVYGETERRSFVQYNFQALLFTL
                     GGILFIILALGGIVVMPAIFAFVGLESVAGWIVSLLRWPVLLAFILLGLALLYRYGPS
                     RETAEWRWVTWGSGFAAVVWLVVSMLFSWYVSSFGNFNETYGSLGAVIGFMIWMWLSA
                     T"
BASE COUNT          713 a         1252 c         1237 g          851 t
ORIGIN      
        1 tgacccacgg acccttcaac atctcgcgct cgatcagctc gaagcaggcg ccgaccgact
       61 gaggcacctt cttcttcatc gcctcgatgg cggccggatc gtctgcccag cgatagccgc
      121 gaactctgtg ggcgtgggcg acgtgaacgg tcgagcacag atagctgttg aactcctgta
      181 gctgcgcaaa cgcgaaggga tcatccagcg gagcgagctt cgcttcggga aagctctgag
      241 cgacgaacgc gagcagggca ggcgtctcgg tgaggatgcc gcgcggcgtc accatggccg
      301 gcactctcgc tttgggattg atcgccagat actcgggcga ctgctgctgt gacttgctga
      361 aatcgatccg cttgagctcg tactcggccc cggcatcgag cagggcgatg tgcgtggcaa
      421 gtgcgcaggt attaggtgcg taatagaggg tgaacatcgt ctgtgctctg attttggatt
      481 ggtaggtcag cctgacatga cgagatcttc acccgtctgg cgatggagca caagacagcg
      541 taatcgagaa ttcaggctga ttggcacgat tcaaaaaacg gggggctgga gcaccgccgc
      601 tgggacgtgc ggattggcca attcgcgggc gtagatcgaa ctggtctggc ctctcagttc
      661 gaccaactgt tcagagcttt gtcgcaatcc ttggtgatcg gctagccaat gaaaaccgcg
      721 cctctatcgg gttaggcccg aatagagacg cgggttccac atatcagtct ggttttcggc
      781 ccacagggcc aggagcactc taccgcgtgc cactgacggc agcggcgacc tcagcggcaa
      841 agtcctcctc tttccgctca atgccctcgc cgagggcgta gcgcacgaaa ccagtaatct
      901 tgacaggcgc gccgaccgtg ccctcggctt ccttgaccgc ctgctcgacg gtcgacttgc
      961 cgtcgatgac gaagatctgc tgcatcaaca gcacctgctg gaagaattcc ttgcgcaggc
     1021 ggccctcggc catctttgca acgatgtcct gcggcttgcc gctcgcagca gcctgctcct
     1081 cgtagatctt gcgctcacgc tcgatcaccg ccggatcaag atcctcggcg gaaatggcca
     1141 gcggtgctgc cgccgcgaca tgcatcgcaa tctggcggcc gaactcggcg agctttgcct
     1201 tgtcgccggt gctctccagc gccacaagca caccgatctt gcccaggccc tcggcgacgc
     1261 ggttgtgcac ataatcaacg agcacgccgt cagcgcctgc ctcgagcgtc gccgtgcggc
     1321 gcacacccat gttctcgccg atggtggcga tcatctcctt cacgtgatcc tcgacggaga
     1381 tagccttacc ggggaacggc gtagccagga gacgagaaag atcgccgtcg gcttggagcg
     1441 cgagcttcga aatatctcgc accatctcct gaaactgggc gtttcgcgcc acgaagtccg
     1501 tctccgaatt gacttcgacc agtgcgccct tccgcccctc gacggcaacg ccgatcagcc
     1561 cctcggccgc gacacggccc gccttcttgg ctgccttgga aagccccttc ttgcgcagcc
     1621 agtcgatgga cgcctcaatg tctccgttgt tctcaatcag tgcctgcttg caatccatca
     1681 tgcccgcgcc ggtcttgtcg cggagctctt tcaccatcgc ggcagaaatc tgcgtcatct
     1741 tcaccctctc gggttaggcg ggccgattgc gcccgcgatt gaactggcag ggtcctgatg
     1801 gagcacctgc cggattttcg ctcagatcag gctgacgcgc ctcaggccgc ggcagcctcg
     1861 accagcttct tggcctggcc gatccagtct tccttctcga tctgaccgtt cagcttaagc
     1921 tgccggtcga gagcctgcat gctctcggta tcgagatcgg caatttgcca aaaatggaaa
     1981 acgcccaggt cattgagccg ttgctcgagc ttggccgaaa tgccgttgat ccgcttgaga
     2041 tcgtcgggct cgccccgcgg cgcttcaatg cctttgaaga caaagggcag cgcctcttcc
     2101 ggcggagtca cggcctcgcc gatgtcaata ccggcagcag cctgagcacg ctggatgccg
     2161 tcgatggcgg cacgtgccgc gagatcgcag tagagcgtga tggcacggcc tgcgtcgtcg
     2221 ttgccgggaa tcgggaagtc aatgccgtcc ggatcgcagt tcgtatcgac gacggcgaca
     2281 atggggatcc cgagcttctt ggcttcctgg acggcgatcg cttccttgtt cgtgtcaatg
     2341 acgaacagga ggtccggcgt gccgcccatc tccttgatgc cgccgagcgc ctgattgagc
     2401 ttgtcgcgct cgcgcgtgag gttcagcacc tctttcttgg tgcggcccac cggatcggcg
     2461 aggatctcat cgagctgacg aagacggcgg atcgactgcg aaatcgtctt ccagttcgtc
     2521 aaggtgccac cgagccaacg gtggttgatg tagtactgcg ccgaccgctt ggcagcctcg
     2581 gcgatggcat cggcggcctg gcgcttcgtg ccgacgaaca gcacacgacc gccttcggcg
     2641 accgtgtcgg aaatcttgac cagcgcctgg tgcagcagcg gcaccgtctg cgtcaggtcg
     2701 atgatgtgga tcttgttgcg gacaccgaag atgtattgcc gcatcttcgg gttccagcga
     2761 tgggcctggt ggccgaaatg cacgccagct tcgagtagct gacgcatggt aaatgttggc
     2821 agcatcgtct ctctcgttct ccggttagac ctccgcggac agtgagcgcc atcatctcca
     2881 ctgagctgat gtacgcaccg gagcgccatc ggcggggctt tcggtccgcc gcagcgcgat
     2941 gatccgcgtg tggaatgagc cgcatttagc cagacttttc cccgatagca aggtggatcg
     3001 cggctgacaa ggggaacagc cgccgcatcc cgcgcgttcg ggcacgagat ggtagcgagc
     3061 gagcacatac ccgcagcatc gccccaccgg cggaagaccg ccccgcagcg cggctctctc
     3121 gcgcttgggc tggccatgct cggcgcaatg acgctgcgcc gacggttcga ccgcggtaag
     3181 gaggcggaag ttggccaagt gagttcgcca agaccaaagc agagcttgga ccgcggcata
     3241 gccccggggc gcggtcgaga agcacaaagc ccgtcggaaa tcccggctgc cggttggaag
     3301 gatgtcttat ggcgggtcta tgagcgcttt caaagcgatc gcgtgatgct gattgccgcc
     3361 ggtgtcacct actacgggct actggccctc tttccggcca ttgcggcgct tgtctcgctt
     3421 tacggactct ttgccgatcc ggacaccatc aagcagcagc tctcgaactt gcaaggggtg
     3481 ctcccggaag acgcgattca gatcatcgga gaccagatca cgcatattca ggcgcagggt
     3541 ggcggcaggt tggggatcta cttcgcgatc ggcctcatcg tctcgctttg gagtgccaac
     3601 gccggtgtga aatcgatgtt cgacgcgctc aatgcagttt acggagaaac cgagcgccgg
     3661 agcttcgtcc aatacaactt tcaggctttg cttttcacgc tcggcggcat tctcttcata
     3721 attttggccc taggcgggat tgtcgtcatg cccgcgattt tcgcattcgt cgggctggaa
     3781 agtgtggccg ggtggatcgt ttcgctgctg cgttggccgg tgttgctcgc cttcatcctg
     3841 ctcgggcttg ccttgcttta ccgctatggg ccgagccggg agaccgctga atggcgctgg
     3901 gtgacctggg gcagcggctt cgccgccgtc gtttggctcg tcgtgtcgat gcttttctcc
     3961 tggtacgttt ctagcttcgg caatttcaac gaaacctacg gctcgctcgg cgccgttatc
     4021 ggcttcatga tttggatgtg gctttcggcg acc
//
LOCUS       DASUMG010000489         2892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_949234, whole genome shotgun sequence.
ACCESSION   DASUMG010000489 DASUMG010000000
VERSION     DASUMG010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949234"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..240
                     /locus_tag="VFX46_08120"
     CDS             61..240
                     /locus_tag="VFX46_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HEX5600586.1"
                     /translation="MTYDTVAQFSQITSLFLFIALFIGVIAYAFWPGNRRRFEAAQRQ
                     ALDLDDDTALRRGRK"
     gene            237..1103
                     /gene="ccoP"
                     /locus_tag="VFX46_08125"
     CDS             237..1103
                     /gene="ccoP"
                     /locus_tag="VFX46_08125"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEX5600587.1"
                     /translation="MSTKEIDEVTGVETTGHEWDGIKELNKPLPKWWVWTFWATIIWS
                     IGYWVLYPAFPTLTGYTKGVLGYSQRAVVAEQIEAARAEQAKFRDALAATPLEQVKDD
                     PELLRFAMAGGAAVFADNCAPCHGRGAQGFPGYPNLNDDEWLWGGSIEDIHNTIRFGI
                     RSGHPEGRDSQMPRFGIDQLLDRNQISDTAEYVLSLSGRATDEAAVARGREIFAEQCV
                     ACHGEDGKGNVELGAPNLADAIWLYGGSKEDIMESIRTGRGGQMPSWEGRLDPVTIKS
                     LAVYVHSLGGGQ"
     gene            1252..2757
                     /gene="ccoG"
                     /locus_tag="VFX46_08130"
     CDS             1252..2757
                     /gene="ccoG"
                     /locus_tag="VFX46_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX5600588.1"
                     /translation="MPQNLKPADFINEAAPGVERHDVAAINRRDRRELYAARQKVYPR
                     LAHGRFRNIKWAVMAITLSVYYLLPWLRWDRGPNLPDQAVLLDFANGRLFLFFLEIWP
                     QELYYITGILVISALSLFLVTALAGRVWCGYACPQTVWTDLMIAVERFWQGDRNARIR
                     LDKEPWTATKIFKKTMTHLSWLLIALATGGALVFYFRDAPTLARELVTGTAPAIAYLF
                     LGIFTFTTYLLGGIAREQVCIYMCPWPRIQGAMVDRHTLLVSYRADRGEPRGPLRKGQ
                     GWEGRGDCIDCKACVAVCPMGIDIRDGLQLECIQCALCIDACNEIMDKVGRPRGLIAY
                     DTIAKKEAKAAGVNHEPLKLVRPRTVLYSCLIVLVAGVMALGLISRTTLEVNVLHDRG
                     VLFVPLSDGGVRNAYSVKVLNKRHEAVNVDLAVEGLPGAQMRIVGHDGNGAISVPPDD
                     VREVRVLVTVPPEHVGDLTPPSTRFDFIVRDAETGTEMRRDAIFQSASPGK"
BASE COUNT          542 a          840 c          910 g          600 t
ORIGIN      
        1 gctggttgat ttcgccagct tcgatgccgc cggccccaac ttgaggtgag ggccagcatc
       61 atgacctacg acactgtcgc acaattcagc cagatcacct cgctgttcct tttcatagcg
      121 ctgttcatcg gggtcattgc ctacgctttc tggcccggaa atcgccggcg cttcgaagcg
      181 gcgcaacgtc aagcgctcga tctcgatgat gacaccgctc tccgcagagg ccgcaaatga
      241 gcacgaaaga aatcgacgag gtcaccggtg tcgagaccac cggtcatgag tgggacggca
      301 tcaaggaact gaacaagccg ctccccaagt ggtgggtttg gacgttctgg gccaccatca
      361 tctggtccat cggctactgg gtgctctatc cagcctttcc gacgctgaca ggatacacga
      421 aaggtgtgct tggctatagt cagcgagcgg tggtggctga gcagatcgaa gctgcgcgtg
      481 ccgaacaagc gaagttccgc gatgcgctcg ccgcgacacc acttgagcaa gtgaaggacg
      541 atccggagct cctgcggttc gcaatggccg gaggcgctgc ggttttcgcc gacaactgcg
      601 cgccatgcca tggccgcggc gcgcaagggt tccccggcta tcctaacctc aacgatgatg
      661 aatggctgtg gggcgggagc atcgaggaca tccacaacac gatccgcttc ggaattcgct
      721 cgggccaccc cgaggggcgt gatagccaga tgccgcgatt tggcatcgac cagctcctcg
      781 atcgtaatca gatctccgat acggcagagt acgtcctgtc tctgtccggc cgggcgacgg
      841 atgaggcggc ggttgcgcgc gggcgggaaa tctttgcgga gcagtgcgtc gcgtgtcacg
      901 gcgaggacgg taaaggcaac gtcgagctag gtgcgccgaa cctcgcggat gcgatctggc
      961 tctatggcgg ctcaaaggaa gatatcatgg agagcatcag gacgggccgt ggcggccaga
     1021 tgccatcatg ggagggccgt cttgatccag tgacgatcaa gtcgctcgcc gtctatgtcc
     1081 actcgcttgg tggcggacaa tgagcggtag ccggactttg tccgaaagca aagcgtagcg
     1141 ccctcgacca tggtctgtag gcgagggtag gtgaggggca gcgaggcgga cgggcctgct
     1201 cctcacctct gatttctccg acgcaggagg gtaaggagac cgatgacctc catgccgcaa
     1261 aatctaaagc ctgccgattt catcaatgaa gccgcgcccg gggtcgaacg gcacgatgtt
     1321 gctgccatca accggaggga tcggcgggag ctttatgcag ctcggcagaa ggtctatccg
     1381 cgcctcgccc acggccgatt ccggaacatc aaatgggcgg tcatggcgat tacgctcagc
     1441 gtttattacc tgctgccctg gctgcgttgg gatcgcggtc caaatttgcc cgaccaggct
     1501 gttttgctcg atttcgccaa tggccgattg ttcctgtttt ttctcgaaat ctggccccag
     1561 gagctctact acatcacagg catcctggtg atttcggccc ttagcctgtt tctcgttaca
     1621 gcgttggcag gccgcgtttg gtgcggctat gcgtgcccgc agaccgtttg gaccgacctc
     1681 atgatcgccg tcgagcggtt ttggcagggc gaccgcaacg cgcgcatccg cctcgataag
     1741 gagccctgga ccgcaacgaa gattttcaag aaaaccatga cacatctctc gtggcttctt
     1801 atcgctttgg ccacgggcgg tgcgctcgtc ttctatttcc gcgatgcacc gacgcttgcg
     1861 agggagctgg tcaccggcac ggcaccggca attgcgtatc ttttcctcgg cattttcacg
     1921 ttcactacgt atttgctcgg cggcatcgcg cgcgagcagg tctgcattta catgtgcccg
     1981 tggccgcgca tccagggcgc gatggtcgat cgccatacgc tgctcgtcag ctacagagcc
     2041 gacaggggcg agccgcgcgg gccactgcgc aaaggccaag gctgggaggg gcgcggcgac
     2101 tgcatcgatt gcaaggcctg cgtggcggtt tgcccgatgg gcatcgatat tcgtgacggt
     2161 ctgcaactcg aatgcattca gtgcgcgctc tgcatcgatg cctgcaacga gatcatggac
     2221 aaggtcgggc gtccgcgtgg cctgattgct tacgacacaa tcgcgaaaaa ggaggccaag
     2281 gctgccggcg tcaaccatga gcccctgaag ttggtccgtc ctcggacagt gctttactcc
     2341 tgcctgatcg tgctcgttgc aggcgtgatg gcgctcgggc tgatttcacg cacgacgctc
     2401 gaggtcaacg ttctgcacga tcgcggcgtg ctgttcgtgc cgctctccga tggcggcgtg
     2461 cgcaatgcgt attcggtgaa agtgctgaac aagcggcacg aagctgtgaa cgtcgatctc
     2521 gccgtcgaag gcttgccggg ggcgcagatg aggatcgtcg gccatgacgg caatggcgcc
     2581 atcagcgttc cgcccgacga tgttcgtgaa gtgcgagttc tggttacggt tccgccggag
     2641 catgtcggcg atctgacgcc accttcgacc cgcttcgact tcattgtcag ggatgctgaa
     2701 accggcaccg aaatgcgccg ggatgccatc ttccaaagcg cgtcacccgg caaatgaggt
     2761 gtggaggcgc gatgatgtca gcgaaacgtt cggaaggact gaccgggcgg catgtgctga
     2821 tcggcgtgct cgcctttttt ggaacggtgt tcttggcgaa cggcattttt ctgtattcgg
     2881 cgctttcgac at
//
LOCUS       DASUMG010000490         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_949532, whole genome shotgun sequence.
ACCESSION   DASUMG010000490 DASUMG010000000
VERSION     DASUMG010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949532"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFX46_08135"
     CDS             complement(<1..270)
                     /locus_tag="VFX46_08135"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX5600589.1"
                     /translation="MAAPPARARPILVTGALGQIGSELVPALRSRYGNDQVIASDVRV
                     EPFAGPTGFPSEHLDCTDISQLQDFVWRHDVGTIYHLAALLSATAE"
     gene            complement(311..1000)
                     /locus_tag="VFX46_08140"
     CDS             complement(311..1000)
                     /locus_tag="VFX46_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX5600590.1"
                     /translation="MKASNPFKDRRSAGKLLAERVKELKPADPVVLAMPRGGVPVGLE
                     VAKALGAPLDLVLVRKIGVPYQPELAAGAVVDGDQPEIVVNDDVMQLAGLTREYIDER
                     AKEELVEIERRRQVYLSGRPRVPLSGRTLIIVDDGIATGASIRAAIAALKRRNPLKLI
                     LAVPVAPADTIEKLRAEVDDVVCLQMPEPFYAIGMHYDDFHQVPDDEVVRLMNEAPAL
                     EPVPAGSNGGS"
     gene            complement(997..1653)
                     /locus_tag="VFX46_08145"
     CDS             complement(997..1653)
                     /locus_tag="VFX46_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX5600591.1"
                     /translation="MTQLEQTREATVEVGSHHLEGILRIPENAIGLVIFAHGSGSSRL
                     SPRNTHVAHQLNSRGLATLLFDLLTEHEAAYRANVFDIPLLGTRVAEAVRWAREKPET
                     ANLDIGLFGSSTGAAAALVAAAQAPDDVKAIVSRGGRPDLAGDYLPSVRAPTLLIVGG
                     ADYGVIELNEQALERLTCEKRLEIIPGATHLFEEPGALDQVVDLTGRWFETHLGRSVQ
                     "
     gene            1817..>3435
                     /gene="polX"
                     /locus_tag="VFX46_08150"
     CDS             1817..>3435
                     /gene="polX"
                     /locus_tag="VFX46_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX5600592.1"
                     /translation="MAVHNAEVAELFNRLADLLEIEGANVFRVRAYRRAAQTIEDLPV
                     SVAEMVEQGQDLSKLPGIGRDLAGKIEEIVKTGKFTALEEVTARLPSSLATLVAVPGL
                     GPKRVQLIHDKLGVRSLEDLAAAARAHKLRTLPRFSPKLEARILEELERHRTTERRFR
                     IATAAEFAEALRAYMQRCPGVDQVVVAGSYRRCKETVGDLDLLVTCAKSKPVIEHFVA
                     YEDVAEIAAKGSTRAAIALKTGLQADIRVVPAKSYGAALHYFTGSKDHNIHIRKLGQA
                     RGLKINEYGIFRDDKRIGGRTEEEVFAAVGLPYIEPELREDRGEIEAAQEGRLPKLVT
                     LADIRGDLHCHTNASDGKNTLVQMVEAARARGYEYLAITDHSKHATVARGLDEKRLAK
                     QLGEIDRLNDSLDGFRVLKSCEVDILADGTLDLPDRILKRLDFVICAVHYKFDMDAKS
                     QTERILRAMDNPYCKILAHPTGRLLLDRAGYALEIERVLRGAKERGCFLELNSHPSRL
                     DLDDVHCKAAKEMGILISIGTDAHSTMGLDA"
BASE COUNT          645 a         1061 c         1095 g          634 t
ORIGIN      
        1 ctcggctgtc gccgacaaaa gcgccgcgag atggtagatg gtgccgacgt cgtgccgcca
       61 aacgaaatcc tgaagctgag agatgtcggt gcagtcgagg tgctcgctgg ggaagccagt
      121 gggcccagca aacggctcga cgcgcacgtc ggaggcgatc acctggtcgt tgccgtatcg
      181 gctgcgtagc gccggcacca actccgaacc gatctgaccc agcgcgcccg taaccaagat
      241 cggccgtgct ctggcaggcg gcgccgccat tccagcgttc tcctcaatcg gatcaggtct
      301 ctaagtcgtg tcaggagccg ccgttgctgc cggcagggac cggctctagc gccggggctt
      361 cattcattaa acgcacgacc tcatcatccg gtacctgatg aaaatcgtcg tagtgcatgc
      421 caatggcgta gaacggctcc ggcatttgca ggcagaccac gtcatcgacc tcagctcgca
      481 gcttctcgat ggtgtctgcc ggtgcgacag gaacggcgag gatcagcttc agcgggttcc
      541 ggcgcttgag ggctgcgatg gcggcccgaa tgctggcccc agttgcaatg ccgtcgtcaa
      601 cgataatgag agtgcgccca ctgagcggca cccgcggccg acctgagaga tagacctgcc
      661 tgcgtcgctc gatttcgacc agctcctcct tggcgcgctc gtcgatgtac tccctggtga
      721 gcccggccag ctgcatcacg tcatcgttaa cgacgatttc cggctggtcg ccatccacga
      781 cagctcctgc ggccagctcc ggctggtaag gcacgccaat cttgcggacg agcacgaggt
      841 ccagcggcgc gccgagcgcc ttggccacct cgaggccgac gggtactcca ccgcgcggca
      901 tcgccagaac gacgggatcg gccggcttca gctccttgac gcgctcggcg aggagcttgc
      961 cggcgctcct gcggtctttg aacggatttg acgccttcat tgcaccgatc tcccgagatg
     1021 cgtttcgaac cagcggcccg tcagatcgac gacctggtcg agcgcgccgg gctcctcgaa
     1081 aaggtgcgtc gcgcccggga tgatttcgag ccgcttctca caggtgagcc gctccagcgc
     1141 ctgctcgttg agctcgatca ccccgtaatc ggcgcctccc actattagca aagtcggcgc
     1201 gcggacgctc ggcagataat cgccggccag atccgggcgg ccgccgcggg agacaatggc
     1261 cttcacatcg tcgggcgcct gcgccgctgc gacgagcgcg gccgcggcgc ccgtgctgct
     1321 tccgaaaagg ccgatgtcga ggttcgccgt ctccggtttc tcccgcgccc agcggaccgc
     1381 ttctgcaacg cgggtgccga gcagcggaat gtcgaaaaca ttggctcgat aggccgcctc
     1441 gtgctccgtc agcaaatcga agagcaatgt ggcaagcccc cggctgttca gctgatgagc
     1501 cacgtgggtg ttgcggggac tgagccggct cgacccgctg ccgtgggcaa agataacaag
     1561 cccgattgcg ttctctggaa tccggagaat gccttcgagg tgatgactgc cgacctcgac
     1621 agtggcttcg cgtgtctgct cgagttgagt catggcggcc tcactggttg ttcgtggagg
     1681 ctcgatcgct ctcagcgact ccgcatcgca tgatggacgt tgggctctgc aacggccgcg
     1741 tgcattgatg gttgtcaacg caagaacacc tgagacgcgc tcgttttcca tcgattgact
     1801 gcgacagagc cgcgccgtgg ccgttcacaa cgcagaggtt gccgagctgt tcaaccgcct
     1861 cgccgatctc ctggagatcg agggggcgaa cgtcttccgc gtgcgagcct ataggcgggc
     1921 cgcacagacg atcgaggatc tgcccgtgag cgtcgccgaa atggtcgagc aggggcagga
     1981 cctctccaag ctccccggta tcgggcggga tctcgccggc aaaatcgagg agatcgtcaa
     2041 gacaggtaaa ttcactgcgc tggaggaggt gacggcgcgc cttccatcga gcctggcaac
     2101 cctcgtggca gtgccggggc ttgggccaaa gcgcgtccag ctcatccacg acaagcttgg
     2161 cgtccggtcc cttgaggatc ttgcggcggc ggcgcgcgcc cacaagcttc gcacgttgcc
     2221 tcggttcagc ccgaagctcg aggctcgcat tctggaggag ttggagcggc accgcacgac
     2281 cgagcgccga ttcaggattg ccacagccgc ggaattcgcc gaggcgctac gcgcctatat
     2341 gcagcgctgt cccggtgtcg atcaggtcgt ggtggcgggc agctacaggc gttgcaagga
     2401 gaccgtcggc gacctggacc tgctcgtgac ttgcgcaaaa agtaagcccg tcatcgagca
     2461 cttcgttgcc tacgaggatg tcgctgaaat cgctgccaag ggctcgacac gcgccgccat
     2521 tgcgctgaaa acgggcctgc aggcagatat tcgtgttgtt cccgccaaga gctatggcgc
     2581 tgcgctgcac tatttcaccg gttcgaagga ccacaacatc catattcgga agctcgggca
     2641 ggcgaggggc ctcaagatca acgagtatgg aattttccgc gacgacaagc ggataggcgg
     2701 gcgcaccgag gaggaagttt ttgccgccgt cgggctgccc tatatcgagc cggagctcag
     2761 ggaggatcgc ggggagatcg aggccgccca ggaaggccgg ctgccgaagc tggtgacgtt
     2821 ggccgatatt cgcggcgatc tgcactgcca caccaatgca tcggacggaa agaatacgct
     2881 ggtgcaaatg gtcgaagccg ccagagcccg cggctacgag tatctggcta ttaccgacca
     2941 cagcaagcac gctacggtcg cgcgtggtct cgatgaaaag cgcctggcga aacagctcgg
     3001 cgagatcgac cgcctgaacg acagcctgga tggcttccgc gtcctgaaat cttgcgaggt
     3061 ggacattctg gcggacggaa cgctggattt gccggaccgc attttgaaac ggctcgattt
     3121 cgtaatctgt gctgttcatt acaaattcga catggacgcc aagtcgcaaa ccgagcggat
     3181 attgcgcgcc atggataatc cctattgcaa aattttggcg catcccaccg ggcgcctgtt
     3241 gctggatcgt gcgggctatg cgttggaaat cgagcgcgtg ctgcggggcg ccaaggaaag
     3301 ggggtgcttt ctggagctga actcccatcc ttcgcgtctc gatctggatg acgtgcactg
     3361 caaagccgca aaggagatgg gcattctcat ttcgatcggc acggacgccc actcgaccat
     3421 gggacttgat gcgat
//
LOCUS       DASUMG010000491         3752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_708234, whole genome shotgun sequence.
ACCESSION   DASUMG010000491 DASUMG010000000
VERSION     DASUMG010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708234"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1437
                     /gene="ccrA"
                     /locus_tag="VFX46_08155"
     CDS             139..1437
                     /gene="ccrA"
                     /locus_tag="VFX46_08155"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HEX5600593.1"
                     /translation="MMGGETIATPAAAPVKKDLYEIGEIPPLGHVPKQMYAWTIRKER
                     HGEPDKAMQVEVVPTWELDSHDVLVLVMAAGVNYNGVWAALGKPISTLDTHKHPYHIA
                     GSDAAGIVWAVGSKVKRWKVGDEVVIHCNQDDGDDEQCNGGDPMFSPSQRIWGYETPD
                     GSFAQFCRVQDRQLMERPKHLTWEESACYVLTLATAYRMLFGHRPHILRPGHNVLVWG
                     ASGGLGSMAVQLCATAGANAIGVVSDDDKIDFVMQLGAKGVINRKKFNCWGAMPKVGT
                     PEYDTWLKEVRAFGKAIWDITGKGNNVDFVFEHPGESTFPVSVFVVKRGGMVVICAGT
                     TGYNLTMDARYLWMHQKRVQGSHFANLLQASQANRLVIERRIDPCMSEVFSWEQIPKA
                     HVRMMRNEHKPGNMAVLVNAPVTGLRTFEDVLEASRVRFG"
     gene            1567..2022
                     /locus_tag="VFX46_08160"
     CDS             1567..2022
                     /locus_tag="VFX46_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600594.1"
                     /translation="MEEEPKSAFRTKVRVKARSGRPDRNATAPELPAPPKKPISLMEA
                     LEQVRASSDGDAEQPNKETSGTKRPARSHEVPQPAETASNNRKSSRPTPARTGRIAAA
                     KLRQLQRLQEAAASLEKLARGEIEEATVEIIRRDSKRPTDNQATNPQKR"
     gene            2201..>3752
                     /locus_tag="VFX46_08165"
     CDS             2201..>3752
                     /locus_tag="VFX46_08165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX5600595.1"
                     /translation="MRDSAQPVSTIPASKQVLSDTDAVILAAEQILQSAKATVRNKLE
                     DVRDLDRCQHAAHGLSWLATYVEAMRQLRGWAQRLSDAGLFGELELLLLKLGIAEYAA
                     QIAGGIPMSQGEFARLGDMGVGASDIHRFMETTAGFVADGTSERVKRRLAELIAELPP
                     GELVGNAGLDDTHAAIQEQMRRFSEAEVAPHAHSWHLANAYIPMEVIAKLSELGVFGI
                     TLPEEYGGLGLGKEAMCVVSEELSRGYIGVGSLGTRSEIASELILGSGTEEQKQRYLP
                     RIASGEILPTAVFTEPDTGSDLASLKTRAVREGDVYKVTGQKTWITHPVRADLMTLLV
                     RTDPNEPGYRGLSMLLAEKPRGTDEDPFPAPGMSGGEIEVLGYRGMKEYDISFDYFEV
                     PVSQLLGGVEGQGFKQLMSTFESARIQTAARAVGVAQSALDHALAYARERIQFGRPIY
                     AFPRVSNKLVMMAVEVMIARQLTYYAARQKDQGRRCDLEAGMAKLLAARVAWSAADNA
                     VQIHGGNGF"
BASE COUNT          765 a         1142 c         1167 g          678 t
ORIGIN      
        1 tcccccgctc ttgcgcggga tccaaagata agctttgccg cgctgtgttg cggtgcaata
       61 atcgggcgct ggaacgcatt cgggcgacac gctttgggaa ggaccatgtc aagaacagat
      121 gcgaccgcaa ccggcaccat gatgggaggg gaaactatcg ccacgcccgc ggcagccccg
      181 gtgaaaaagg acctttacga aatcggcgag atcccgccgc tcggccatgt gccgaagcaa
      241 atgtatgcct ggaccatacg caaagagcgg cacggagagc ctgacaaggc catgcaggtc
      301 gaggtcgtgc ccacgtggga gctcgacagc cacgacgtgc tggtgctcgt gatggcagca
      361 ggcgtcaact acaacggggt ctgggcagcc ctcggcaagc ccatatcgac gctagacacc
      421 cataagcacc cctaccacat cgccggctcc gacgcggcgg gtatcgtttg ggcagtcgga
      481 tcgaaggtga agcggtggaa ggttggcgac gaggtcgtca ttcactgcaa ccaggacgat
      541 ggggacgatg agcagtgcaa cggcggcgat ccaatgttct cgccctcaca acgtatctgg
      601 ggctacgaaa cgccagatgg ctcgttcgct cagttctgcc gcgttcaaga ccgacagctc
      661 atggagcggc cgaagcatct cacctgggag gaaagtgcct gctatgtgct gacgctggcc
      721 accgcctatc gcatgctgtt cggccatcgc ccgcacatcc tccgtcctgg gcacaacgtg
      781 ctggtgtggg gagcctccgg cggactgggc tccatggccg ttcagctgtg tgccaccgcg
      841 ggcgccaacg ccatcggcgt cgtgtcggac gacgacaaaa ttgattttgt tatgcagctc
      901 ggcgctaagg gcgtcatcaa tcgcaagaag ttcaactgct ggggcgcgat gccgaaagtt
      961 ggcacaccgg agtacgatac ttggctcaag gaggtgcgtg ccttcggcaa ggcgatttgg
     1021 gacatcactg gcaaggggaa caacgtcgac ttcgttttcg agcacccagg agaatcgacc
     1081 ttcccggtgt ccgtcttcgt cgtaaagcgc ggcggcatgg ttgtcatctg cgccggcacg
     1141 accggctaca atctgaccat ggacgcccgc tatctctgga tgcaccagaa gcgcgtccag
     1201 ggttcacact ttgccaatct gctgcaagct tcgcaggcta atcggctggt gatcgagcgg
     1261 cgaatagacc cgtgcatgtc ggaggttttc tcttgggagc agattccgaa ggcgcacgtt
     1321 cgaatgatgc gcaacgagca caagccgggg aacatggcgg ttctggtgaa cgcgccggtg
     1381 accggtctgc gcacgtttga ggacgtgctc gaggcctccc gcgttcgctt cggctaagtc
     1441 cgtccgtatt cttcgcaatt cggcaagctg taggcggtta cgatcaccaa ggtagccgct
     1501 tgcattggct gtcagggctg tgacctactc acaacaggta ctgtcgtccg cgctggaggg
     1561 agccaagtgg aagaagaacc taaaagcgca ttccggacca aagttcgggt gaaggctcgg
     1621 agcggcaggc cagaccggaa cgcgaccgcc cccgagttgc ccgcgccgcc gaaaaagccc
     1681 atttcgctga tggaggctct tgagcaagtc cgcgcatcat ctgacggtga tgcggagcaa
     1741 cccaacaagg agacctccgg gacgaagcgg ccggcgcgct cacacgaagt gccacaaccg
     1801 gctgaaacgg caagcaacaa tcgcaaatca tcacgcccaa cccccgcgcg aaccggacgg
     1861 atcgcggcag ccaagctacg gcagctccaa cgtcttcagg aggctgccgc cagccttgaa
     1921 aagttggctc gtggcgagat cgaggaagct acggtcgaga tcatccgtcg agacagcaag
     1981 cgccctaccg ataaccaggc caccaatccc cagaagcgct gacccaacct gccgaacctg
     2041 ttgcatggtg tcccggttca taaccggaac gttatagcgt tattgcgttc ttttgtgcga
     2101 tgcagcatac gctagccgca taggggaacg tgcggcgcgc ccccttcacg cagtgcaacg
     2161 ggaggagctt cgggatcgcc gcttagcagg atctgatttg atgagagatt cagcacagcc
     2221 ggtttctaca attcctgctt ccaagcaagt tctgtccgac acagacgcag tcattctcgc
     2281 ggcagagcag atcttgcaat ccgccaaggc gactgtgcgg aacaagctcg aggatgtccg
     2341 cgatctcgac cgctgccagc atgcggctca cggactttct tggctggcca cctacgtgga
     2401 agccatgcgt cagctcaggg ggtgggctca gcgactgagc gatgcgggat tgttcggcga
     2461 gctcgagctg ttgctgctca agctgggcat tgcggagtac gccgcccaga ttgccggcgg
     2521 cattcccatg agccagggag aatttgcccg gctcggagac atgggcgtcg gagcctctga
     2581 tattcaccgt ttcatggaaa caaccgccgg ctttgttgcg gacggcacga gcgaacgggt
     2641 gaagcgccgc cttgccgagc tcatcgccga gctgccacca ggggagcttg tcggcaacgc
     2701 gggcctggac gacacccacg ctgctatcca agagcagatg cgccgcttca gcgaagccga
     2761 ggtcgcgccg catgcgcatt cttggcacct ggccaatgcc tacatcccca tggaggtcat
     2821 cgccaagctc agcgagcttg gcgtgttcgg catcacgctg cccgaggaat atggcggcct
     2881 cggccttggc aaggaggcca tgtgcgtcgt ctccgaggag ctatcgcgcg ggtacatcgg
     2941 cgtgggctcc cttgggacgc gttcggaaat cgcgagcgag ctgatcctcg gcagcggaac
     3001 cgaagagcag aagcaacgct atctgccgcg gatcgcctcc ggagaaattc tgccgaccgc
     3061 cgtattcacc gagccggaca cgggctctga tctcgcctct ctcaagaccc gcgcggttcg
     3121 cgagggagat gtctacaagg tcacggggca gaagacctgg attacacatc cggtgcgcgc
     3181 cgatctcatg acgctcctcg tgcgcacaga tccgaatgag cccggctata ggggtctttc
     3241 gatgttgctc gccgaaaagc ctcggggaac agatgaagac ccgttcccag caccaggcat
     3301 gagcggcggc gagatcgagg tgctcggcta tcgcggcatg aaggagtacg acatctcctt
     3361 cgactacttc gaggttccgg tttcacagct ccttggaggc gtcgaggggc agggcttcaa
     3421 gcagctcatg tcgaccttcg aatctgcccg cattcagacg gcggcccgcg ccgtcggtgt
     3481 cgcccagtcg gcgctcgatc acgcacttgc ctacgcccgg gagcgcatac agttcggccg
     3541 cccgatttac gcgtttccac gcgtttccaa caaactcgta atgatggctg tcgaggtcat
     3601 gatcgcccgc cagctcacct attacgctgc ccggcaaaag gatcagggcc ggcgctgcga
     3661 cctcgaggca ggcatggcca agctgctggc cgcgcgcgtc gcttggtccg ccgcggacaa
     3721 tgccgtgcag atccatggcg gcaacgggtt cg
//
LOCUS       DASUMG010000492         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1191069, whole genome shotgun sequence.
ACCESSION   DASUMG010000492 DASUMG010000000
VERSION     DASUMG010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191069"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFX46_08170"
     CDS             <1..935
                     /locus_tag="VFX46_08170"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX5600596.1"
                     /translation="SVIFFTTASGAGYGLLIWLGVFGPLGWVPQTSAFGLVGLGLALL
                     LITGGLLSSTAHLGHPERAWRALSQWRTSWLSREGVAAIATYVPAGLFGIGWVFLERT
                     AGFFALMGVLAAAMAVATLICTGMIYASLPTIRAWHQPFVVPVYIALALATGAILLNF
                     LLLLFGITTGWAPLIAILAIAAAWILKTASWSRVDNAERSYTIEQATGLGRFGKVRVL
                     EPPHTQPNYVMREMGYQIGRKHAQKLRRIAVVLTFIVPGLLSLLMLVIGAQLGLLLAL
                     VAVVSAGIGVLIERWLFFAEAEHVVMLYYGKEAA"
     gene            complement(1013..1345)
                     /gene="hspQ"
                     /locus_tag="VFX46_08175"
     CDS             complement(1013..1345)
                     /gene="hspQ"
                     /locus_tag="VFX46_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HEX5600597.1"
                     /translation="MSKIRVAKYAIGQVVRHRFFPFRGVIFDVDPVFNNTEEWWLSIP
                     EEIRPSKDQPYYHLLAENAETEYVAYVSEQNLMLDDSGIPLRNPKVQELFAETGNGRY
                     HATFVLPN"
     gene            complement(1480..2532)
                     /locus_tag="VFX46_08180"
     CDS             complement(1480..2532)
                     /locus_tag="VFX46_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEX5600598.1"
                     /translation="MPATRTPAKFYAPLAVGAPQPLRQLPVRLERMIHFIPPHNAKIR
                     ARLPETIASVDVLLGNLEDAIPADEKEAARRGFIEMAEQHDFGSTSLWVRINALNSPW
                     ALDDILEIVPSVGSKLDVMMLPKVEGPWDIHYLDQLLAQLEAKHRIEKPILIHAILET
                     AEGVSNVEEIAAASPRMHGMSLGPADLAASRGMKTTRVGGGHPDYGVLADAAGSGAPR
                     AFYQQDLWHYTIARMVDACVRNGIKPFYGPFGDFSDDAACESQFRNAFLMGCVGAWSL
                     HPKQIAIAKRVFSPDPSEVAFAKRILDAMPDGTGAVMIDGKMQDDATWKQAKVIVDLA
                     RLVKEKDPELAASYSI"
     gene            complement(2603..>2945)
                     /locus_tag="VFX46_08185"
     CDS             complement(2603..>2945)
                     /locus_tag="VFX46_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HEX5600599.1"
                     /translation="IITDYVRRGGRLGLVCDLHDRTGIPVPFFGKPARTQAIAAMIAR
                     RVGARIWLARCLRIGNESRFRIQIKELKVPRSSNPADDIRAIMTEMQAQFELWVREAP
                     EQWMWSNRRWS"
BASE COUNT          489 a          921 c          920 g          615 t
ORIGIN      
        1 actcggtaat ctttttcacg acagcatcag gagccggtta cggcctcctc atctggctgg
       61 gagtcttcgg cccgctcggg tgggtgccgc agacgtctgc cttcggcttg gtcgggctag
      121 ggctggcgct tctgctcatc accggaggct tgctgtcgtc gacggcgcac ctcggtcatc
      181 cagagcgagc gtggcgggcg ctctctcagt ggcggacctc ctggctatcc cgcgaaggcg
      241 tggcggccat agcaacctat gttccggcag gactgttcgg catcggttgg gttttcctgg
      301 agcggaccgc tggttttttc gccctgatgg gtgtgctggc cgcggctatg gccgtggcga
      361 cgctcatctg cacgggaatg atctacgcgt cacttcccac aatccgtgcg tggcaccagc
      421 cgttcgtggt gcccgtttac attgcgcttg ccctggcgac gggagccatt ctgctgaact
      481 tcctccttct cctatttggc atcacgacgg gctgggcgcc tctgattgcg atcctggcaa
      541 tcgcggcggc ctggatactc aagaccgcgt cttggtcgcg ggtcgacaac gcggagcgct
      601 cgtacacgat agagcaggcg acgggcctcg gccggtttgg caaggtccgc gtgctcgagc
      661 cgccgcatac gcaacccaac tatgtcatgc gcgaaatggg ctaccagatc ggccgcaagc
      721 acgcgcaaaa gctgcgccgc atcgccgtgg tcctgacctt catcgtgccg gggctgctgt
      781 ctcttctgat gctcgtgatc ggcgcgcagc tcggcttgct cttggccctc gttgcggtcg
      841 tatcggcagg tattggcgtg ctgatcgagc gctggctgtt cttcgcagaa gccgagcacg
      901 tggtcatgct gtattacggg aaggaagcgg cctaagccgc tcgcgcaagc tcggcgcgag
      961 gcccctcata ccggggccag gccgttcctc gcgaattgcg gtatgcgctc gatcagtttg
     1021 gtagcacgaa ggtggcgtga taccggccgt tccccgtttc tgcgaacagc tcctgcacct
     1081 tcgggttcct caagggaatg ccgctgtcat ccagcatgag gttctgctcg gagacgtagg
     1141 cgacatactc cgtctcggca ttttccgcca gcaagtgata gtagggctgg tccttgctcg
     1201 ggcggatctc ctccgggatc gacagccacc actcttcggt gttgttgaac acggggtcca
     1261 cgtcgaagat gacgccgcga aacgggaaga atcggtggcg gaccacttga ccgatcgcgt
     1321 acttggccac gcgaattttg ctcatgacag cttgggtacc agagctccct ggctggcacc
     1381 cttcgagcgt gcactggcgc cagccccccc aattcgtgca cccgattgaa gatagctgat
     1441 tagcgctccc ctgttgttgc agcaagcgca caacttgtct caaatcgaat atgacgccgc
     1501 caactcggga tccttctcct taacgagccg ggcaaggtcg acaatgacct tcgcctgctt
     1561 ccaggtggca tcgtcttgca tcttgccgtc gatcatgacg gcgccggtgc catccggcat
     1621 cgcgtccaga atgcgtttcg cgaaagccac ttcgctcgga tcggggctga agacgcgctt
     1681 ggcgatcgca atctgtttcg gatgcagcga ccacgcgcca acacagccca tcaggaaagc
     1741 attgcggaac tgactttcgc atgccgcatc gtccgagaag tcgccgaagg ggccgtagaa
     1801 cggtttgatg ccattgcgca cgcacgcgtc aaccattcgc gcgatcgtgt agtgccacag
     1861 gtcctgttga tagaacgcgc gcggggcgcc gcttccagcg gcatcggcga gtacgccgta
     1921 atcgggatgg ccacctccga ccctggtcgt cttcatgccc cgcgatgcag cgaggtcggc
     1981 cggccccagg ctcatgccgt gcatgcgggg gctcgcggct gcgatctcct cgacgttgga
     2041 gacgccttcc gccgtctcca agatggcatg gatcagaatc ggcttctcga tgcggtgctt
     2101 tgcttcgagc tgcgcgagca gctggtcgag ataatggatg tcccagggcc cttccacctt
     2161 gggcagcatc atcacgtcca gcttggagcc gacggacggc acaatctcca ggatatcgtc
     2221 gagtgcccag ggggagttga gggcgttgat gcgcacccaa aggctcgtgc tgccgaagtc
     2281 gtgctgctca gccatctcga tgaagcctcg gcgcgccgcc tctttctcgt cggccggtat
     2341 tgcatcctcc aggttcccga ggagcacgtc cacactggcg atcgtttccg gcaaacgggc
     2401 gcgaatcttg gcgttgtgcg gcgggatgaa gtggatcatc cgctcgagcc tgacggggag
     2461 ctggcgcagc ggctgcggtg cgccaacggc gagcggtgca tagaacttgg ctggcgtgcg
     2521 ggtggctggc attgagctcc tcgatccgac actttcttcg tatcaacgcc ggatgactac
     2581 catccgtctc gcagggggcc aatcagctcc agcgccggtt cgaccacatc cactgctcgg
     2641 gcgcctcccg gacccacagc tcgaactggg cttgcatctc ggtcatgatc gccctgatgt
     2701 catcggccgg gttggaggag cgcggcactt tcaattcttt gatttgaatg cggaaacggc
     2761 tttcgttgcc gatgcgcagg cagcgcgcga gccagatgcg cgcccccacc cgacgcgcga
     2821 tcatcgcggc gattgcctgc gttcttgccg gcttgccgaa gaacggtacg gggatgccag
     2881 tgcggtcgtg aagatcgcaa acaagcccta agcgtccgcc gcgccggaca taatccgtga
     2941 tgatc
//
LOCUS       DASUMG010000493         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_388443, whole genome shotgun sequence.
ACCESSION   DASUMG010000493 DASUMG010000000
VERSION     DASUMG010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388443"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFX46_08190"
     CDS             <1..767
                     /locus_tag="VFX46_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HEX5600600.1"
                     /translation="IDPKLQTYLSSRVLSIAANEMGHEVVMAPCNGCYHNLKKAEYDL
                     AHDSESVEVTERLSKKAGHATYRAGQVETIHALDWLKATVGEDGIKERTKGKLAGLKV
                     ANYYGCMYVRPRHIFPEKDKGPGSESTTKPHFMDDLLAAAGAENVDFPLKTACCGGAH
                     TLSDSDTSTKLVLNILQAAEASGADVIATECPTCHTGLEMHQVRAEKRFGKKTNVKIM
                     YFTQLLGLALGIRPRKLGIQSNISDGMELVVEKRLT"
     gene            776..1195
                     /locus_tag="VFX46_08195"
     CDS             776..1195
                     /locus_tag="VFX46_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600601.1"
                     /translation="MRSFAEIEAEIDGIANAGLTPQEAVARLVAIGEEILEHWIIARG
                     ETPTSEQKEGFRLLALHRQGAKGDPSFNACRETARELAYHYNLITLEPDHPDTPKRVM
                     LAAMVARHLCLFISGKMEVAGLGEFCCSSRPLRAAGG"
     gene            1261..1704
                     /locus_tag="VFX46_08200"
     CDS             1261..1704
                     /locus_tag="VFX46_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEX5600602.1"
                     /translation="MPVVKGCNLPDDLLYDVDNHIWFKEEGDGNVRLGMTTVATAMAG
                     QLVAFTPKRVGRKVDAGKSCATVESGKWVGPAKSAAAGEVVQVNDDLIANPQLANQDP
                     YDKGWFIILKPENWDAVKATLTPGTQVAAKYEAKMNAEGFGGCAP"
     gene            1738..>2111
                     /locus_tag="VFX46_08205"
     CDS             1738..>2111
                     /locus_tag="VFX46_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX5600603.1"
                     /translation="MGQQFASDNNAGICPQALARVLEANAAGHATGYGDDAWTQEARE
                     RIAALFEADCDVYFVFNGTAANALALAQLCRPYNAIIAHAFSHIETDEAGAPEFFTGG
                     AKVMTADTPLAKLTPEAVEALAT"
BASE COUNT          435 a          632 c          676 g          368 t
ORIGIN      
        1 acatcgatcc caagctgcag acctacttga gctcgcgcgt gctctctatc gctgccaacg
       61 aaatgggcca tgaggtggtg atggcgccct gcaacggctg ctatcacaac ctcaagaagg
      121 ccgagtacga cctggcgcac gactcggaat cggtggaggt tacggagcgg ctgtccaaga
      181 aggctggtca cgccacctat agggcgggcc aggtggagac catccacgcg ttggactggc
      241 tcaaagcaac ggtcggtgag gacggcatca aggagcgcac aaaaggtaag ctcgccgggt
      301 tgaaggtcgc caattactac ggctgcatgt acgtccggcc gcggcatatc tttcccgaga
      361 aggacaaagg ccctggctcc gagtcgacca caaaacccca tttcatggac gatttgcttg
      421 ccgccgctgg cgccgagaat gtcgactttc cgctgaaaac cgcctgctgc ggcggcgcgc
      481 acacgctttc ggatagtgat acgtcgacga agcttgtgct gaacatccta caggccgccg
      541 aagcctccgg ggccgatgtc atcgccaccg aatgcccgac ctgtcatacg ggactggaaa
      601 tgcatcaggt gcgcgcagag aagcggttcg gcaagaagac caacgtcaag atcatgtact
      661 tcacccagct cctcgggctt gcgctgggca tccggccgcg caagctcggc atccagtcga
      721 acatttccga cgggatggag ctcgtcgtcg agaagcggct gacctgagtc tgattatgcg
      781 ctcattcgcc gagatcgagg ccgagatcga cggaattgcc aatgcagggc tgacgccgca
      841 ggaggctgtg gcccggctcg tcgcgatcgg cgaggagatc ctggagcatt ggatcatcgc
      901 acgtggcgaa acgccaactt cggagcagaa ggaaggattc cgcctgctgg cgctgcatag
      961 gcagggagcc aagggcgacc cgtcgttcaa tgcctgccgc gagacggccc gcgagcttgc
     1021 ctatcactat aacctgatca cactcgagcc cgatcatccc gacacgccca agcgggtcat
     1081 gctcgctgcg atggtcgctc ggcatctctg cctgttcatc tccggcaaga tggaggtcgc
     1141 aggcctcggc gagttctgct gctcatctcg gccattgcga gccgccggcg gttgatcggc
     1201 cttgcagatt cgacgcgaac gcgcctgtaa tgtctccgac gaactgctca gaggacttcc
     1261 atgcccgttg ttaaaggatg caatctgcct gacgatcttc tttacgacgt ggacaaccac
     1321 atctggttta aggaagaggg cgacggcaac gtgaggctcg gcatgacgac cgtcgccaca
     1381 gcgatggccg gccagctcgt agccttcacg ccgaagcggg tcgggcgcaa ggtcgacgcg
     1441 ggtaagagtt gcgcgacagt cgaaagcggc aagtgggtgg ggcctgccaa gtctgcagcg
     1501 gctggtgagg tggtgcaggt gaacgatgat ctcattgcca acccgcagct cgccaaccaa
     1561 gacccttacg acaaggggtg gttcattatc ctgaagccag aaaactggga tgcggtgaaa
     1621 gcgaccctca cgcctggtac tcaggtggcg gcgaaatacg aggctaagat gaatgctgag
     1681 ggcttcggcg ggtgcgcgcc gtaacgtcga cagcttagtg tcaattgcga ccagcccatg
     1741 ggccagcagt ttgcgtccga caacaacgca gggatctgcc cgcaggcgct ggcgcgggtc
     1801 ctcgaagcca acgctgccgg gcacgccacc ggctacggcg atgatgcctg gacgcaggag
     1861 gcgcgggagc ggatcgcggc gctgttcgag gcggactgcg atgtgtattt cgtcttcaac
     1921 ggcacggccg cgaatgctct ggcgctggcc cagctctgcc ggccctacaa cgccatcatc
     1981 gcgcacgcgt tctcacacat tgagacggac gaggctggcg caccggaatt cttcaccggc
     2041 ggcgccaagg tgatgacggc cgatacgcct ctggcgaagc tgacgccgga ggcggtggag
     2101 gcgctggcaa c
//
LOCUS       DASUMG010000494         2096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1191521, whole genome shotgun sequence.
ACCESSION   DASUMG010000494 DASUMG010000000
VERSION     DASUMG010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191521"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..152)
                     /locus_tag="VFX46_08210"
     CDS             complement(<1..152)
                     /locus_tag="VFX46_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX5600604.1"
                     /translation="MRSSRVITVVGCHAEGEVGDVIIGGVLPPAGATMLEKKLTMERE
                     HDHIRR"
     gene            complement(165..644)
                     /locus_tag="VFX46_08215"
     CDS             complement(165..644)
                     /locus_tag="VFX46_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX5600605.1"
                     /translation="MRARLDVIDWRILKELQADGRVTNVALAQRVGISAPPCLRRVRA
                     LEEAGFITGYHAQVDEQMLGFELTAFAMVGLHSQAEPDLRDFENRILGWPLVREAYMM
                     SGETDYVLKCVAPDLTTFQNFIIRELTAAPNVASVKTALAIRRAKREPGVPIGYPRS"
     gene            817..1839
                     /locus_tag="VFX46_08220"
     CDS             817..1839
                     /locus_tag="VFX46_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HEX5600606.1"
                     /translation="MQPQDQIETDRPQGVLSGARAWIITDGKAGMVVQCKGVADALGL
                     DYEMKCVKTRGIWRALPPWGPVQPADRPGRPGALLSPPWPVIAIGSGRASIPYIRAIR
                     RYSEGATFTLILQDPRTGAGVADLIWVPEHDRLRGSNVVVTVTSPHSYSPERLARLRR
                     ELPPEIEALPHPRVAVVLGGKNGSYKFTDADDDRLAKALESIGRLGASFMITPSRRTH
                     SRLLRVADKATQNRPRILWDGTGENPYPHFLAAADWLVVTADSVNMTGEACATGRPVY
                     VFEPSRGSRKFRYFHQRLQGIGATRPLPPVVEQLESWSYAPIDSAAVIAREVERRYLL
                     RFGRTD"
     gene            1908..>2096
                     /locus_tag="VFX46_08225"
     CDS             1908..>2096
                     /locus_tag="VFX46_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600607.1"
                     /translation="MHLNLDRLRAAKIETDPYEFVIVPGFLSAESLRRINETFPRIER
                     GGSYPIEALEAGMTIKEVI"
BASE COUNT          391 a          630 c          674 g          401 t
ORIGIN      
        1 atgcggcgga tgtggtcgtg ctcgcgctcc atcgtgagct tcttctcgag catcgtggcg
       61 cctgcaggcg gcagcacgcc accgataatg acatcgccca cctcgccctc ggcgtggcag
      121 ccgacgacag tgatgacgcg ggaggaacgc atacggagct cctgtcagga tcgggggtag
      181 cctatcggca cgcccggctc acgtttggcc cgccggatcg cgagcgctgt cttcacgctt
      241 gcgacgttgg gggcggcagt cagctcccgg ataatgaagt tctggaacgt agtgaggtcg
      301 ggagctacac acttcagcac ataatccgtt tcgccagaca tcatgtaggc ctcccggacc
      361 agtggccagc cgagaatacg gttctcgaag tcgcgcaagt cgggttctgc ctggctatgc
      421 aacccgacca tggcgaaggc cgtcagctcg aaaccgagca tctgctcgtc gacttgagcg
      481 tgatagccgg ttatgaaccc agcttcctcg agggcgcgaa cgcggcgcag gcagggtgga
      541 gcgctgatgc cgacgcgctg agcaagtgcc acgttcgtga cacggccgtc ggcctgcagc
      601 tccttgagga tgcgccagtc gatgacgtcc aggcgggcac gcatgggatt gaggctcgtt
      661 tgacaacagg gcttggcaga gccgcttcat gctggatagg acccttgccg gcgaatggca
      721 actactgccg gcgcttcggc ctgcctcgga aaggctctag cgcgtgcggc ggcgggcagc
      781 atcgacaggg tgcagcccta tcggcgagaa ggcgatatgc agcctcaaga tcaaatcgag
      841 accgatcgcc cacaaggtgt gctctcagga gcgcgtgcct ggatcatcac cgacggaaaa
      901 gccgggatgg tcgtccagtg caaaggcgtc gctgatgccc taggcctgga ctatgagatg
      961 aagtgcgtga aaacgcgcgg catttggcga gcgcttccgc cttgggggcc agtgcaacct
     1021 gccgatagac ccggccgtcc cggcgctctg ctctcgcccc cgtggccggt aatcgccatc
     1081 ggcagcggac gtgccagcat tccatatatc cgggcaattc ggcgctactc agaaggagcg
     1141 acattcacct taatccttca ggatccgcgg actggggcag gggttgcgga tctcatctgg
     1201 gttccggagc acgaccggct ccgcggatct aacgtcgtcg ttaccgttac ctcgccgcat
     1261 agctattcgc ccgaacggtt ggcacgactg cggcgggaat tgccacccga gatcgaggct
     1321 ttgccccatc cgcgggtggc ggtcgtgctt ggcgggaaga atggcagcta caagtttaca
     1381 gacgccgatg acgatcgcct tgcaaaggcg ctcgagtcca ttggccgcct aggcgccagc
     1441 ttcatgatta caccttcgcg gcgcacgcat tcccgcttgc tgcgtgtggc tgataaggcg
     1501 acccagaaca ggccgcgcat tttgtgggat ggcacgggcg agaacccata cccccacttc
     1561 ttagcggctg ccgattggct ggtcgtaacg gccgattccg tgaacatgac cggcgaagcc
     1621 tgcgccactg gtcggccggt ttacgtgttc gaaccctccc gcggcagccg caagttccgt
     1681 tacttccacc agagattgca ggggataggc gctacacgcc ccttgcctcc cgtagtcgag
     1741 cagcttgaat cttggagcta cgctccgatc gactctgcgg ctgtcatcgc aagggaggtg
     1801 gagcggcggt atctgcttcg cttcggccgc acagattgac ggaggggcga tcagcaggtc
     1861 accgggcgtt ggcttattcg gggggagaga aaggttatga ggccttggtg catctcaatc
     1921 tggaccggct ccgcgctgcc aaaatcgaga ctgatcccta tgagttcgtc attgtacccg
     1981 gtttcctctc ggcggagagc ctgcgccgca taaacgagac gtttcccagg atcgagcggg
     2041 gcggctctta tccaatcgag gcccttgaag cggggatgac gatcaaggag gtgatt
//
LOCUS       DASUMG010000495         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_67209, whole genome shotgun sequence.
ACCESSION   DASUMG010000495 DASUMG010000000
VERSION     DASUMG010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67209"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VFX46_08230"
     CDS             complement(<1..538)
                     /locus_tag="VFX46_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX5600608.1"
                     /translation="MLAIEGEAHALADRLPDLLIEAIRVASTVAHGIHGRRRPGLGET
                     FWQFRQFQTGDAAAQIDWRRSASSDHLYVREREWEAAHTIWLWPDQSPSMDFRSHLSA
                     TSKADRALVLALASAELLVRGGERVALLGLSRPTASRKATTRLAEILAENADSPVLQS
                     SLPPAERLSRFSGVLLFSD"
     gene            complement(597..1532)
                     /locus_tag="VFX46_08235"
     CDS             complement(597..1532)
                     /locus_tag="VFX46_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX5600609.1"
                     /translation="MSRVHKATASVIFGQDTVIEQTLITLLAGGHGLLIGVPGLAKTL
                     LVETLGKVLGLEAKRIQFSPDLMPSDIIGSEVLEDIPGERRSFRFVPGPIFTQLLMAD
                     EINRASPKTQSALLQAMQEHHVTVAGQRYDVPMPFHVLATQNPLEQEGTYPLPEAQLD
                     RFLLQIDVSYPDVVAERRMLFATTGIEERRLEPILTAQELMAMQRLVRQIPVPDKVVE
                     AILQLVRSARPGPDASEETNRLIAWGPGPRASQALMLAVRAKALIDGRLAPSVDDVIA
                     LAEPVLKHRMALSFVARTEGQEIGSVISRLASALE"
     gene            1743..>2043
                     /locus_tag="VFX46_08240"
     CDS             1743..>2043
                     /locus_tag="VFX46_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEX5600610.1"
                     /translation="MMQQATGGSTRIQGLDELIKAAEGDKLPPVENWNPPDCGDIGMR
                     IRADGTWLYQNSPIGRKALVKLFSRILRKDADGRTYLVTPVEKVVVAVDDAPFLAV"
BASE COUNT          379 a          625 c          670 g          369 t
ORIGIN      
        1 agtcgctgaa caggagcacg cctgaaaagc gactcagccg ctcagcaggc ggcaagctcg
       61 actgcagcac cggggaatcg gcattctctg cgaggatttc cgccagcctg gtcgttgcct
      121 tgcggctggc cgtgggtcgc gacaagccga gcagggccac gcgttcaccg ccgcgtacca
      181 gcagctcagc cgatgcgagt gccaggacca gagcccggtc cgccttggac gtcgccgaaa
      241 gatggctgcg gaaatccatg gacggtgact gatccggcca gagccaaatg gtgtgcgccg
      301 cttcccattc gcgctcgcgc acgtagaggt gatcggagct tgccgagcgg cgccaatcaa
      361 tttgggcggc cgcgtcgccg gtctggaact gccggaactg ccagaaggtt tcgccgagcc
      421 ccggccgtcg gcggccgtgg atgccgtgtg ccaccgtgct cgccacgcgt atggcctcga
      481 tcagcagatc aggaagcctg tccgcaagcg catgcgcctc gccttcgatg gcgagcatcg
      541 agcctgcgtg cgcggacccg cgtgcgtttt tcgtggccac aggctttgcc accgcactac
      601 tccaatgctg acgcgagccg tgaaatgacg ctgccgatct cctgcccttc ggtgcgcgcc
      661 acgaagctca atgccatgcg atgtttcagc acgggttcgg caagcgcgat cacgtcatcc
      721 accgacggcg cgaggcggcc gtcgatgaga gctttggcgc ggaccgctag catcagtgcc
      781 tggctggcgc gaggtcccgg tccccaggcg atgagccggt tcgtctcctc gctggcatcc
      841 ggcccgggac gcgccgagcg gacgagctgc aaaatggctt cgacgacctt gtccggcacg
      901 ggaatttggc gcaccaggcg ctgcatcgcc ataagctcct gcgccgtcag gatcggctcg
      961 agccggcgct cctcaatgcc ggtggtcgcg aacagcatgc ggcgttccgc cacaacatcg
     1021 ggatagctga catcgatctg gagcaggaag cggtcgagct gtgcctcggg caggggatag
     1081 gtgccctcct gctcgagcgg gttttgggtg gcgagcacgt ggaacggcat cggcacgtcg
     1141 tagcgttggc cagcgaccgt gacgtgatgc tcctgcatgg cctgaagcag tgcggactgc
     1201 gtcttggggc tcgcgcggtt gatctcgtcg gccatcagga gctgtgtgaa gatcggaccg
     1261 ggcacgaagc ggaagctgcg ccgttcgcca gggatgtctt cgagaacctc ggaccctatg
     1321 atatccgacg gcatcaggtc tggcgagaac tggatccgct tggcttcgag gccgagcact
     1381 tttccaagcg tctcgacgag caaggtcttg gccagacccg gcacaccgat gagcagcccg
     1441 tgcccgccag caagcagcgt gatgagagtt tgctcgatca cggtgtcctg gccgaagatc
     1501 acggatgccg tggctttgtg gacgcgggac atgcgctcgc cggccgcctc gatgcgggcg
     1561 acgatatcgt catcggtgtg ggtgagcgtg gtcatgatct ctactataga ttgggtccgg
     1621 tgttgcacaa actctgctgc agcgaaattg gtcacaatat ggagcgaggt gaacaaatat
     1681 gaagggttcc gagtaacttg ctcatctcgc aataaattga acgagcactg cgcgagaacc
     1741 cgatgatgca gcaagcaacc ggcgggagca cccgaattca aggcttagac gagcttatca
     1801 aggcggctga aggcgataag ctcccgcccg tcgagaattg gaacccgcca gattgcggcg
     1861 acatcggcat gcgtatccgg gcggacggga catggctcta ccagaatagc cccataggcc
     1921 gcaaagccct cgtgaaactg ttctcgcgca ttctccgcaa ggatgccgac ggccgcacgt
     1981 atctcgtcac gcctgtagag aaggtggttg ttgccgttga cgacgcgccg ttcctcgctg
     2041 tcg
//
LOCUS       DASUMG010000496         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1031589, whole genome shotgun sequence.
ACCESSION   DASUMG010000496 DASUMG010000000
VERSION     DASUMG010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031589"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VFX46_08245"
     CDS             <1..1159
                     /locus_tag="VFX46_08245"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004690368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX5600611.1"
                     /translation="AEALGMSLPGCAAIPAPHRDRAQMAYLTGKRAVEMVFEDLRPSK
                     ILTRAAFENAIVVNSAIGGSTNAPIHLNAIARHVGVELDNDDWERIGYDIPLLVNLQP
                     AGEYLGEDFYRAGGVPAVVAELIEQGKIREDAITVNGKTLVENCKGRFSTDRRVIKPY
                     NEPLKKQAGFLNLKGNLFDSAIMKTSVISEEFRQRYLSNPNDPNAFEGRVVVFDGPED
                     YHARIDDPSLEIDENTLLFIRGTGPVGYPGGAEVVNMRAPDYLLKKGVRSLPCIGDGR
                     QSGTSGSPSILNASPEAANNGGLALLRTGDRVRIDLNKRTANILISDEELEQRRKELM
                     AKGGYKYPESQTPWQEIFRSLVDEQSRGAVLKNAVKYQRIAETKGIPRDNH"
     gene            1171..1614
                     /locus_tag="VFX46_08250"
     CDS             1171..1614
                     /locus_tag="VFX46_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX5600612.1"
                     /translation="MGPKATTATMISAMRYDDPAAAVDWLCRVFGFKPHAVYKDDAGN
                     VVHAELSFGNGMIMLGPNIDSPFGRYMTMPKDAGGRCTQTVYVIVDDVDAHHAHASAA
                     LADVVMPLRDESYGGREYSCRDPEGHVWTFGNYDPWTHASNTAAA"
     gene            complement(1637..>2284)
                     /locus_tag="VFX46_08255"
     CDS             complement(1637..>2284)
                     /locus_tag="VFX46_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600613.1"
                     /translation="RSSQPWDGNDMDTYADDLAQLIEALDLRNVILIGHSTGGGEVTR
                     YIGRHGTSRVAKVALVSAVPPLMLQTPDNPGGLPMSVFDGLRAASLADRSQLYRDLAS
                     GPFFGFNRPGANVSLGAIESFWHQGMMGGFKNTYDCIKAFSETDFTEDLKKIDVPTLI
                     IHGDDDQIVPIDAAGRASAKIVKNSELKVYPGGAHGLAVTAREQLTNDLLAFISA"
BASE COUNT          441 a          716 c          734 g          393 t
ORIGIN      
        1 cgccgaggcg ctcggcatga gcttgccggg ctgcgcagcg atcccggcgc cccaccgcga
       61 ccgcgcgcag atggcctatc tcacgggcaa gcgggccgtc gagatggtgt tcgaggactt
      121 gcggccttcg aagatcctga cgcgcgccgc cttcgagaac gccatcgtcg tcaactcggc
      181 gatcggcggt tcgaccaatg cgcccatcca tctcaacgcg atcgcgcggc acgttggcgt
      241 cgagctcgac aacgacgact gggagcggat cggttacgac atccccctcc tcgtcaacct
      301 gcagccggcg ggagagtacc tgggcgagga cttctaccgc gctggcggcg ttcctgccgt
      361 ggtggccgag ctgatcgagc aaggcaaaat ccgcgaggac gcgattaccg tgaacggcaa
      421 gacgctggtc gagaattgca aagggcgctt ctccaccgac cggcgcgtga tcaagcccta
      481 caacgagccg ctcaagaagc aggccggctt cctcaacctg aagggcaacc tgttcgacag
      541 cgccatcatg aagacgtcgg tcatcagcga ggaattccgg cagcgctacc tctccaatcc
      601 gaacgatccc aacgcattcg agggcagggt cgtcgtgttc gacggtccgg aggactatca
      661 cgcccgcatc gacgatccgt cgctcgagat cgacgagaac acgctgctgt tcatccgcgg
      721 cacggggccg gtcggctatc cgggcggcgc cgaggtggtg aacatgcggg cgcccgatta
      781 tctgcttaag aagggggtgc ggtcgctgcc gtgcatcggc gatggtcgcc agtcgggtac
      841 gtcgggcagc ccgtccatcc tcaatgcgtc gcccgaggcg gccaacaacg gcggtctggc
      901 gctgctgcgg acgggcgacc gcgtgcgcat cgacctcaac aagcggacgg ccaacatcct
      961 catttcggac gaggagctgg agcagcgccg caaggagctg atggccaagg gtggctacaa
     1021 gtatcccgag agccagacgc cctggcagga gatcttccgc agcctcgtcg acgagcagag
     1081 ccgcggcgcg gtgctcaaga acgcggtgaa gtatcagcgg atcgccgaga cgaagggcat
     1141 tccgcgcgac aatcactgat tggagacgtc atgggaccga aggcgacgac tgcgacgatg
     1201 atctcggcca tgcggtacga cgacccagcg gccgcggtgg actggttgtg tcgcgtcttc
     1261 ggtttcaagc cgcacgcggt ctacaaggac gatgcgggca acgtcgttca tgccgagctg
     1321 tcgttcggca acggcatgat catgctgggt ccgaacatcg acagtccgtt cgggcgctac
     1381 atgaccatgc cgaaggacgc gggtggacgc tgcacgcaga cggtctacgt catcgtggat
     1441 gacgtggacg cccatcacgc tcacgcgagc gcagccctgg cggacgtcgt catgccactg
     1501 agggatgagt cctacggcgg ccgtgagtat tcctgccgcg atcccgaagg tcacgtctgg
     1561 accttcggga attatgatcc gtggacgcac gcatcaaata cggcggccgc ataagattgg
     1621 agcggttgcg cccctctcag gcactgatga acgcaagcag gtcgttggtc agctgctcgc
     1681 gcgcggtgac cgccaatccg tgggcgccgc cagggtaaac cttgagctca gaattcttca
     1741 cgatcttcgc cgaggctcgt ccagccgcat cgatcggcac gatctggtcg tcatcgccgt
     1801 ggatgatcag cgtcggcacg tcgattttct tcagatcctc ggtgaaatca gtttccgaga
     1861 aggccttgat gcagtcgtag gtgttcttga acccacccat cataccttga tgccagaagc
     1921 tctcgatcgc tcctagcgag acgttggctc ccggccgatt gaaaccgaag aacggcccgg
     1981 aggccaaatc ccggtaaagc tgcgatcgat cggcgagcga cgccgcacgc aacccgtcaa
     2041 atacgctcat cggaaggccg cccggattat caggtgtctg cagcatgagc ggcgggacgg
     2101 cgctcaccag cgcaactttg gccacgcgac ttgtaccatg gcgcccgatg tagcgggtga
     2161 cctcgccgcc accggtcgag tgcccgatga ggatgacatt gcgcaggtca agcgcctcga
     2221 tcagttgggc aagatcgtcg gcgtacgtgt ccatgtcgtt gccgtcccat ggctggctcg
     2281 atcg
//
LOCUS       DASUMG010000497         3205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1191998, whole genome shotgun sequence.
ACCESSION   DASUMG010000497 DASUMG010000000
VERSION     DASUMG010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1191998"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /gene="glmU"
                     /locus_tag="VFX46_08260"
     CDS             <1..459
                     /gene="glmU"
                     /locus_tag="VFX46_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947555.1"
                     /note="forms a homotrimer; catalyzes the acetylation of
                     glucosamine-1-phosphate and uridylation of
                     N-acetylglucosamine-1-phosphate to produce UDP-GlcNAc;
                     function in cell wall synthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX5600614.1"
                     /translation="IEGARLRRGAEVGPFARLRPNADIGPRAKIGNFVEVKNVTVEEG
                     AKANHLSYLGDGRVGAGANIGAGTIFCNYDGFFKHRTDVGAGAFVGSNTALVAPVNIG
                     PGAFIGSGSVITKDVPADALALERNEQSVREGWAAKFRTMMQRRKSEKRS"
     gene            559..2379
                     /gene="glmS"
                     /locus_tag="VFX46_08265"
     CDS             559..2379
                     /gene="glmS"
                     /locus_tag="VFX46_08265"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008510617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX5600615.1"
                     /translation="MCGIVGILGSKPVAGDLVDALRRLEYRGYDSAGIATVENGHLDR
                     RRAEGKLKNLEMRLREQPLQGRAGIGHTRWATHGRPIERNAHPHMNGRVAVVHNGIIE
                     NFQELKDELAAKGHEFQTDTDTETVVHLVTDYLDQGHSPIEASRLALQRLRGAFALGL
                     IFAGHDDLMIGARHGPPLAIGHGEGEMYLGSDAIALAPFTGAITYLEDGDWAVITRKG
                     AEIYDSSGSRVERPMVKSVASSLLVDKGNYRHFMAKEIHEQPEVISHTLANYLDMSSG
                     RVMFPDLGVDLAKVSRITLTACGTAYYAGLIGKYWLERFARLPVEIDVASEFRYREAP
                     LPEDGLAIFVSQSGETADTLASLRYCKAQGQRIASIVNVHSSTIARESHTVLPTLAGP
                     EIGVASTKAFTCQLSVLACLAIAAGRARGHISAEQEKELVRALTEVPRHITTILRTEP
                     QYESLAVSLHKARDVLYLGRGQNYPIALEGALKLKEISYIHAEGYAAGELKHGPIALI
                     DENVPVVVVAPEDDLFEKTVSNMQEVAARGGRIILISDAPPAKAGCNLQTHLQVPRAH
                     PFTAPLIYAVPVQLIAYHTAVVMGTDVDQPRNLAKSVTVE"
     gene            2495..>3205
                     /locus_tag="VFX46_08270"
     CDS             2495..>3205
                     /locus_tag="VFX46_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5600616.1"
                     /translation="MTRARTTRAGLTSYSKLTLTAVLAAGLILGVPGAEAAKSKDGAA
                     NADHLIKTGVDALRAGRVDLAVQELTAAITGGKISQSTLARALYYRGLAYRKQNKPAQ
                     AIADFTSALWIKNGLDAEQRADALLNRAAAYREAGLSDQAEADEKRVAATRQAAGANT
                     ASAESRPTPSTSPTASAAPSSSSGGLGSFFSALFGNAPASQEAPAQGSSAPSNSASGT
                     RQATGAGRSPTVSAWSSQT"
BASE COUNT          627 a          981 c         1014 g          583 t
ORIGIN      
        1 atcgaagggg cacgcttgcg ccggggtgcc gaagtcggac cgtttgcccg gctccggccg
       61 aacgcagaca ttggcccacg tgcgaagatc ggcaatttcg tcgaggtcaa gaacgtaacc
      121 gtcgaggagg gcgcaaaggc caatcacctt tcctacctgg gcgacggccg cgtcggagct
      181 ggtgcaaaca tcggtgcagg aactatcttc tgtaactacg acggcttctt caaacaccga
      241 acggatgtgg gggcgggcgc cttcgtcggc tcgaacacgg cgttggtggc gccggtcaac
      301 atcggtccgg gagccttcat cggctcgggc agcgtcatta ccaaagatgt tccggcagat
      361 gccctagcgc ttgagcgtaa cgagcagagc gtgcgcgaag gctgggcggc caaattccgc
      421 acgatgatgc aacggcgcaa atcggagaag cgttcttagc tcgaagacac taagaaatac
      481 caagaaatag ggtttgagtt gtgcccggtg ccgtccagaa ttaggtccag gactgcacaa
      541 acttaaggga ttttcgggat gtgcgggatc gtgggcattc ttggctcgaa gcccgtggcg
      601 ggggatcttg tcgacgccct ccgacggcta gagtaccgcg gctacgactc ggctggtatc
      661 gcgacggttg agaacggtca cctcgaccgc cgtcgcgcgg agggcaagct caagaacctc
      721 gagatgaggt tgcgggagca gcccctgcag ggtcgcgccg gcatcgggca tacgcggtgg
      781 gcgacacacg gccggccgat cgagcgcaat gcccatccgc atatgaacgg ccgggtcgcc
      841 gtcgttcaca atggcattat cgagaatttc caagagctga aggacgagct cgccgccaag
      901 ggtcacgagt tccagacgga tacggacacc gagaccgtcg tccatctcgt tactgattat
      961 ctggaccagg gtcactcgcc catcgaagcg tcacgcctcg cgctacagcg cttgagaggg
     1021 gcgttcgcgc tagggctcat ttttgccggc cacgacgatc tgatgatcgg tgcgcgtcat
     1081 ggcccacccc ttgccatcgg ccacggcgag ggtgagatgt atctcggctc cgacgccatt
     1141 gcgctggctc cgttcacggg cgccatcact tatctcgagg atggcgattg ggccgttatc
     1201 acccgcaagg gtgccgagat ctacgacagc tccggaagcc gggtcgagcg gccaatggtg
     1261 aaatccgtgg ccagctcatt gctggtggac aaaggcaatt accggcactt catggccaag
     1321 gagatccatg agcagccgga ggtcatcagc cacaccctcg ccaactacct ggacatgtcc
     1381 tctggcaggg tgatgttccc cgacctcggg gtcgatttgg ccaaggtctc tcgcattact
     1441 ctcacagcat gcgggactgc gtactacgcc ggactgatcg gtaaatattg gctcgagcgg
     1501 tttgcgcgcc ttccggtcga gatcgacgtc gcctccgagt tccgctaccg ggaggctccg
     1561 ctgccggagg atgggctcgc catcttcgtt tcgcagtcag gcgagacggc cgacaccttg
     1621 gcctcgctgc gctactgcaa ggcgcagggc cagaggattg cgtcaatcgt caatgtgcac
     1681 agctcgacca ttgcgcgtga gtcgcacacg gttctgccga ctttggcagg cccggagatc
     1741 ggtgtcgcct cgacaaaagc cttcacgtgt cagctgtccg tgctcgcatg tctcgccatc
     1801 gcggccggtc gtgcccgtgg gcatatcagc gcagagcagg agaaggagct cgtccgcgct
     1861 ctgacggagg tgccgcggca catcacgacc attctgcgca ccgagccgca gtatgagtcg
     1921 ctggccgtct cgctccataa agcgagggac gtgctctacc tcggccgtgg tcaaaactat
     1981 ccgatcgcac tcgagggagc gctgaagctc aaggaaatct cctacatcca cgcggaaggc
     2041 tatgcggctg gggagctgaa gcacggaccc atcgcgctca ttgacgagaa cgtgcccgtc
     2101 gtggtcgtgg cccccgagga cgatctgttt gagaaaaccg tctccaatat gcaagaagtg
     2161 gcggctcgcg ggggacggat catccttatt tcggacgcgc cgcccgcgaa ggcgggatgc
     2221 aatctacaga cccaccttca agttcctcgc gctcatccgt tcacggctcc gttgatttat
     2281 gccgttcccg tgcagctgat cgcgtatcat accgccgtcg tcatggggac ggacgtcgac
     2341 cagcctcgca acctcgcaaa gtccgtaact gtggaataag gtcggggtcg gacgggattc
     2401 ggcttgtcgc cgaattgcct caaaccggtg attggcatag gaagcacgcg agtggaggtg
     2461 ctgcgcgtac ctgagatgct gcgagggaat cgcgatgacg cgagcgcgca cgacccgggc
     2521 aggattgaca agctattcga aactgacgct cactgccgtc ctggctgctg gccttatcct
     2581 cggcgtgccc ggagcggagg ctgccaaaag caaagacgga gcagccaacg ccgaccatct
     2641 catcaagacc ggcgtggatg ccttgcgggc cgggcgcgtg gatttggccg tgcaggagct
     2701 caccgcggcc ataaccggcg gcaagatctc tcaatcaaca ctcgcgcggg cgctctatta
     2761 ccggggcctc gcttaccgca agcagaacaa gccggcacag gccatcgccg atttcacgag
     2821 tgccctttgg atcaagaatg gcctcgatgc cgagcagagg gcggacgctt tgctgaatcg
     2881 cgccgccgct taccgcgagg caggtctgtc ggatcaggca gaggctgacg agaagcgggt
     2941 ggcggcgacc cggcaagcag ccggcgccaa cacagcttcg gctgagagcc ggccaacacc
     3001 gtcaacgtct ccgacagctt ccgcggctcc atcctcgtcg agcggagggc tcggtagctt
     3061 cttcagtgct ctgttcggca atgccccagc atcgcaagag gcgcccgctc aaggctcctc
     3121 ggcaccttcg aacagtgcat cgggcactcg gcaggcgact ggggctggcc gttcacccac
     3181 cgtgtcggcc tggtccagtc agacc
//
LOCUS       DASUMG010000498         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_869855, whole genome shotgun sequence.
ACCESSION   DASUMG010000498 DASUMG010000000
VERSION     DASUMG010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869855"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..744
                     /locus_tag="VFX46_08275"
     CDS             46..744
                     /locus_tag="VFX46_08275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5600617.1"
                     /translation="MFIPVYDQNPLRSIHFQVVTVSLIAINIVIFILQVTALPLQVVL
                     GFALVPADLVGMGAAVPGAAPSVGEIAIPERLTLLSYMFFHGDILHLAGNMLFLWVFG
                     DNVEDALGHFRFLIFYLACGVAGGLAHALMMPNSELPLIGASGAVAGVIAAYLMLHPR
                     VRIWVLVMRFIPIRISAAFALGAWVVTQLVMAVLPQVGPVAWWAHVGGLAAGAVLVIF
                     MRRSGVPLFDRGIA"
     gene            969..1724
                     /locus_tag="VFX46_08280"
     CDS             969..1724
                     /locus_tag="VFX46_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5600618.1"
                     /translation="MKVLVPVKRVVDFNVKIRVKPDGSGVELANVKMSMNPFDEIAVE
                     EAIRLKEKGVASEIVAVSIGPSKAQETIRTALAMGADRGILIETDEGVTVEPLAVAKL
                     LKAVVQEEQPSLVILGKQAIDDDCNQTGQMLAALLGWPQATFASKLVIEDGDARVTRE
                     VDGGLETLKLKLPAVVTTDLRLNEPRYASLPNIMKAKKKPLDVKKVADFGVDIAPRLK
                     VLKTSEPPARKAGVKVSSVAELVEKLRTEAGVI"
     gene            1724..>2202
                     /locus_tag="VFX46_08285"
     CDS             1724..>2202
                     /locus_tag="VFX46_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX5600619.1"
                     /translation="MAVLVLAEHTNDTFNAVTAKAVTAAAALGGEVHILVAGSNCRPA
                     AEGAAKIEGVSKVLVADAPHLANQIAEEMAALIVPLMANYDALVAPSTARGKNILPRV
                     AAMLDVMQISDITAVKSPDTFERPIYAGNAVQTVQSTEPKKVITVRTAAFAAAPEGGS
                     "
BASE COUNT          395 a          621 c          724 g          462 t
ORIGIN      
        1 aagcggtcaa tttagcttgc gtgcaaactt ccgaggggcg ggcgcgtgtt tattccggtc
       61 tacgatcaaa atccgctgcg ctcgatccac tttcaggtcg ttactgtcag cctcatcgcc
      121 atcaacattg tgattttcat cctgcaggtg acggcgctgc cgctgcaggt ggtgctcggt
      181 ttcgcgctgg tgcctgctga tctcgttgga atgggggcgg ctgtgcccgg tgctgctcca
      241 tctgtcggcg aaattgccat tccggagcgg ctgacgctcc tctcctacat gttcttccac
      301 ggcgacatcc tgcatctcgc aggcaacatg ctgtttttgt gggtgttcgg cgacaacgtg
      361 gaggatgcgc tcggccattt ccgctttctc atattctatt tggcttgcgg agttgctggg
      421 gggctcgcgc acgcgctgat gatgccgaac tccgagttgc cgctcattgg tgccagcgga
      481 gccgtggccg gcgtcattgc ggcttatttg atgctgcacc ctcgtgttcg catctgggtg
      541 ctggtgatgc ggttcattcc catccgcatt tctgccgcct ttgcactcgg cgcttgggtt
      601 gtgacccagc ttgttatggc ggtcttacct caggtggggc cggtcgcctg gtgggcgcac
      661 gtcggcggcc ttgctgcggg agccgttctg gtcatcttca tgcgccggtc cggtgtgccg
      721 ctattcgatc gcggaatcgc atagcagcat caaccagaac gaataagtgc gagtcatcct
      781 gccaaccgaa tgattgactt gctgaaaacg tgagcatacg gtgccttttc gcatgtgcga
      841 aggatgcgga ctgcgccggg ctggtttcgg ggattgagac gcttcatccc ggaatgcgcc
      901 atttctgaaa ggctcgcggg cagcgcgacc gcacgccgat tactggagaa ttaggagaac
      961 tcgggatcat gaaagtcctc gtgcccgtca agcgcgtggt tgatttcaat gtgaaaatcc
     1021 gagtgaagcc ggacggctcg ggggtggagc tcgccaacgt caagatgtcg atgaatccct
     1081 tcgacgagat cgcggtcgag gaggcgatcc ggttgaagga gaagggcgtc gccagcgaga
     1141 tcgtggctgt atcgatcggg cccagcaagg cgcaggagac gatccgcacg gcgttggcca
     1201 tgggcgcgga tcgcggcatt ttgatcgaga cggatgaggg tgtgactgtc gagccgctgg
     1261 ccgtcgccaa gctgctcaag gccgtggttc aggaggagca gcccagtctt gtcattctcg
     1321 ggaaacaggc catcgacgac gactgcaatc agacgggcca gatgttggcg gctctactgg
     1381 gatggcccca ggcgacattc gcctccaagc tcgtgatcga ggacggcgat gcccgagtga
     1441 cgcgtgaggt cgatggcggc ctcgagacgt tgaagttgaa gcttcctgcg gtcgtgacga
     1501 cggatttgcg tctcaacgag ccgcgctatg cctctctgcc caacatcatg aaggcgaaga
     1561 agaagccgct ggatgtgaag aaggtggctg acttcggcgt agacattgcg ccgcgcctca
     1621 aggtcctgaa gacatccgag ccgccggccc gtaaggctgg cgtgaaggtg tccagcgtgg
     1681 cggagctggt cgagaagctc agaacagaag cgggggtaat ctgatggcag tgctggtcct
     1741 tgcggagcac acgaacgaca ccttcaacgc ggtaacggcg aaggcggtta cggccgcggc
     1801 agcactgggc ggtgaggttc acattctcgt ggcggggtcc aactgccgtc cggcggcgga
     1861 gggggctgcc aagatcgaag gcgtttcaaa ggtcctcgtc gccgatgccc cccatttggc
     1921 aaatcagatc gcggaggaga tggcggcgct catcgtgccg ctgatggcca actacgatgc
     1981 actcgtcgcc ccttcgaccg ctcgcggcaa gaacattctg ccccgcgtcg ccgccatgct
     2041 cgatgtcatg cagatctccg acatcacggc ggtgaagtcg cccgatacct tcgagcggcc
     2101 gatctacgcg gggaatgccg tccagacggt gcagtcgacc gagcccaaaa aggtcatcac
     2161 ggtccgcacg gctgcgttcg cggcagcgcc ggaaggggga tc
//
LOCUS       DASUMG010000499         2644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_468261, whole genome shotgun sequence.
ACCESSION   DASUMG010000499 DASUMG010000000
VERSION     DASUMG010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468261"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1443
                     /locus_tag="VFX46_08290"
     CDS             223..1443
                     /locus_tag="VFX46_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX5600620.1"
                     /translation="MPDDKAEKIALFRYGLIAPLVLETLPRGELTRRAQEIATRLYDI
                     PHSTRRQVSVDTLLEWTLRYRRNGLPALMPKPRQDRGQMRAIPDATAALIDRLKRESP
                     HRTGTALLNHLALAGDQAEISPSTLYRFLRARGLTQRQLLLDKASAHKKYEAEFANQI
                     WQSDMLFGPWVQRPGGGKMQVFLQAALDDASRLIPHAQFYPNQGLDSFLDCLRQAIAA
                     RGLPTRLYMDNAKIYRSPQLARIAASIGILIIHTPPYQPEGRGKIERFFRSVREQFLS
                     NLDPRTLLSLDQLNERLWHWLDTVYHRREHSALQTTPLLRWQQDIAQVRQLPPATDMR
                     RLFFHRVDRLVRRDSTFLLRHRFFEAPPHLAGNKIEVRFDPLDLSCPEIYCAGKPEGV
                     ARLVDAVVNGRTYR"
     gene            1443..2243
                     /locus_tag="VFX46_08295"
     CDS             1443..2243
                     /locus_tag="VFX46_08295"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX5600621.1"
                     /translation="MWESFFGFKKTPFSDSPDGKQLFASAAWQQVKTRLEFLMRHPGV
                     GLLTGEVGAGKSTAARVFTAALNPNLYKVLYVHFSSGTALDLLRQIALTLDLEPAHFR
                     GDLVRQIADAVVRLNQSKKQHPILICDEAHLLPHPALEQLPLLLNFDMDSTRYLTLLL
                     VGQPLLRRTLSLQMHEALRQRIGTHYHLEGLSRDELDGYLAQQLKAAGVTQPLFDDTA
                     RQGLYQATKGIPRKVNKLAMTALRLAAARQSQVVDEALLLDATAEALL"
BASE COUNT          482 a          939 c          712 g          511 t
ORIGIN      
        1 gcgctcacca tgcttcgcac aacaggttgg atccccgccc atcgtttcct cttcgccgtc
       61 cttcgacaac atctgctggg ctggtcgggc tcgctcattc ccgacggccg ccgcgtcacg
      121 attccgcccg ccgccgccac cggatgagcc tccccacaca ccatttgtct ggaaccgaac
      181 tgcctttcgc cctacgctcg acccaaggag aaggcagact caatgcccga cgacaaagcc
      241 gaaaagatcg ccctgttccg ctacggattg attgccccgc tggtgctgga aaccctgccg
      301 cgcggcgaac tcacccggcg cgctcaggaa atcgccacac gcctctacga tattccgcac
      361 tccacccgcc gccaggtctc ggtggatacg ctgctggagt ggacgctgcg ctaccggcgc
      421 aacggcctcc ccgcactcat gcccaagccg cgccaggatc gcggccagat gcgcgccatc
      481 cccgatgcca ccgccgcgct catcgaccgg ctcaaacgcg aaagcccgca tcgcacggga
      541 acggcgctgc tgaaccatct ggccctggcc ggtgatcaag ccgagatctc accgtccaca
      601 ctctatcgct tcctccgcgc ccgcggactc acccagcgtc agttgctgct cgataaagcc
      661 tccgctcaca aaaagtacga agccgagttc gccaaccaga tctggcaatc cgacatgctc
      721 ttcggcccct gggtgcaacg cccaggcggc ggcaagatgc aagtcttcct gcaagccgcg
      781 ctcgacgatg ccagccgcct gatcccgcac gcgcagttct atcccaacca gggcctggac
      841 tctttcctgg actgcttgcg gcaagctatc gccgcgcgcg gtctgcccac tcgcctctac
      901 atggataacg ccaagatcta tcgctccccg caactggcgc ggatcgccgc ctctatcggc
      961 atcctcatca tccacacccc gccctatcaa cccgaggggc gcggcaagat cgagcgcttc
     1021 ttccgttccg tgcgcgagca gtttctctct aacctcgatc ccagaacttt gctctccctc
     1081 gatcagttga acgaacggct ctggcactgg ctcgataccg tctatcaccg ccgcgagcac
     1141 agcgcgcttc aaaccacgcc cctgttgcgc tggcagcagg acattgccca ggtgcgtcaa
     1201 ctgccgccgg ccaccgacat gcgccgcttg ttcttccatc gcgtcgaccg cctggtgcgc
     1261 cgcgattcca ccttcctgct gcgccatcga ttctttgaag caccgccgca tctggccggc
     1321 aataagatcg aggtgcgttt cgatccgctc gatctctcgt gcccggaaat ctactgtgcc
     1381 ggaaaaccgg agggcgtggc gcgcctggtg gatgcggtcg tcaacggaag gacctaccgg
     1441 taatgtggga atccttcttc ggcttcaaga agacgccgtt ttccgacagc cccgacggca
     1501 agcaactgtt cgcatcggcg gcctggcagc aggtcaaaac gcggcttgag tttctgatgc
     1561 gccatcccgg cgtgggcttg ctcaccggcg aagtcggcgc cggcaagtcc accgcggcgc
     1621 gagtttttac cgccgccctc aatcccaacc tttataaggt gctctacgtc cacttctcct
     1681 ccggcacggc gctggatctg ttgcgccaga tcgcgctcac gctggacctg gagccggccc
     1741 actttcgcgg cgacctggtc cgccagatcg ccgacgccgt ggtgcgcctc aatcaaagca
     1801 agaagcagca tccgattctc atctgcgatg aggctcactt gttgccgcat ccggcgttgg
     1861 agcagttgcc gctgttgctg aacttcgaca tggactccac gcgctacctc acgctgctgc
     1921 tcgtcgggca gccgctgttg cgccgcaccc tttcgctgca gatgcatgag gctttgcgcc
     1981 agcgcatcgg cacgcactac cacctggagg gactttcgcg cgacgaactc gacgggtatc
     2041 tggcccagca actcaaagct gccggcgtca ctcagccctt gttcgacgac accgctcgcc
     2101 aaggtctgta tcaagccacc aagggcattc cccgcaaggt caacaaactg gccatgacgg
     2161 cgctgcgcct ggccgcggcg cgccaatctc aagtagtcga cgaagcgctc ctgctggatg
     2221 ctaccgccga agcgttgctg tgaaggagtc tctgtaacga tcttccgtcc ggggtcgcgc
     2281 ctggatggaa cccaaggccg aggcgacggg ctgactccgt tactgatcgg catggcaaca
     2341 acccccgagc acgtctcggg ggttttgctt ttgggcatca gggtggtgat gtccccgagc
     2401 cgaggccttg ggcttcaaac gcgaagatcg ccgcaggcgc tccttattct tgcctttgcc
     2461 tcgtcccaaa ctcgcccgcc attagcttga ccaacttgcc ggaaaacaaa tcgagaatct
     2521 cagggggaaa gatcgtgacc cgttacaatt catcgcccgt cgctctggtt ttgatcgatg
     2581 tcatcaatca tttcgaattt ccggacggcg acaagattct caggcacgcg ctacctttgg
     2641 cgag
//
LOCUS       DASUMG010000500         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_468762, whole genome shotgun sequence.
ACCESSION   DASUMG010000500 DASUMG010000000
VERSION     DASUMG010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468762"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="nuoE"
                     /locus_tag="VFX46_08300"
     CDS             complement(<1..569)
                     /gene="nuoE"
                     /locus_tag="VFX46_08300"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX5600622.1"
                     /translation="MAVRRFDPNQPASFEFTPENLAWAKETIAKYPPGKQASAVIPLL
                     WRAQEQHGGWLPEPAVRYVADMLGMAYIRVYEIATFYTMFQLSPVGRKAHVQVCGTTP
                     CMLRGSEALIEICKRRIHPEPHHLSEDGDFSWEEVECIACCANAPAVQIGKDTYEDLT
                     PELFEKVLDGFARGEPPKPGSQIGRQASCP"
     gene            complement(797..1201)
                     /locus_tag="VFX46_08305"
     CDS             complement(797..1201)
                     /locus_tag="VFX46_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HEX5600623.1"
                     /translation="MAQRGQGRLTDVIVWLAIATGVVLSLIGARFLLQPEHAATFFGI
                     DRHNPGFAPHAAIALRDLWLGLLMIAFAVLRDWRAVALWFSLATLVCFGDAVIAATSS
                     GRWISVAFHSASGLFCGAVAAYAWRLARPSAQ"
     gene            complement(1342..2532)
                     /locus_tag="VFX46_08310"
     CDS             complement(1342..2532)
                     /locus_tag="VFX46_08310"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX5600624.1"
                     /translation="MPETDIRPFTINFGPQHPAAHGVLRLVLELDGEVVTRVDPHIGL
                     LHRGTEKLIESRTYLQNIGYFDRLDYVAPMNQEHAFCLAVERLLGIEVPYRAQLIRVL
                     YCEIGRLLSHLLNVTTQAMDVGALTPPLWGFEEREKLMIFYERASGARMHAKYFRFGG
                     VKADLPPDLIEDIAKFCETHPKVLDDIHDLVMENRIFKQRNVDIGVVSLEDCMKWGFT
                     GVMLRGSGAAWDLRKAQPYECYADLEFDIPVGKNGDNYDRQLIRMEEMRQSVRIMKQC
                     CEKLLSAEGQGPHIAQDPKVVPPRRAEMKRSMEALIHHFKLYTEGIHVPPGEVYAAVE
                     APKGEFGVYLVSDGSNKPYRCKIRAPGFAHLQAMDFMNRGHMLADVSAILGSLDIVFG
                     EIDR"
     gene            complement(2548..>2696)
                     /locus_tag="VFX46_08315"
     CDS             complement(2548..>2696)
                     /locus_tag="VFX46_08315"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546747.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX5600625.1"
                     /translation="YVEVRYDDEQKRVVYEPVRLTQEFRDFDFESPWEGTGRLLPGDE
                     KAGS"
BASE COUNT          517 a          873 c          838 g          468 t
ORIGIN      
        1 gggcaggaag cctgacggcc aatctgcgag ccgggcttgg gcggctcacc tctcgcgaag
       61 ccgtcgagaa ccttctcgaa cagctccggc gtcaaatcct cgtacgtgtc tttgccgatc
      121 tgcactgcgg gcgcattggc gcagcaggcg atgcactcca cctcttccca agagaagtcg
      181 ccatcttccg agaggtgatg cggctccggg tgaatgcgcc gcttgcagat ctcgatcagc
      241 gcctccgagc cgcgcaacat gcacggcgtt gtgccgcaaa cctggacgtg cgccttgcgc
      301 ccgaccggcg acagctggaa catggtgtag aaggtggcga tttcatagac ccggatgtac
      361 gccatgccca gcatgtccgc gacgtagcgg acagcgggct cgggcaacca gccaccgtgc
      421 tgctcctgag cccgccagag caacgggatc accgccgagg cctgcttacc cggcgggtac
      481 tttgcaatgg tctccttggc ccaggcgaga ttctcgggcg tgaactcaaa gctcgcaggc
      541 tgattcggat cgaaacgtct gacggccatg gaagcctcga aaccaaggag ataggcaagg
      601 ttcgctcacg accgcagaga tcgcgcgccg cagtggaggc caccccggcg gagcctggct
      661 tctcccgaga cgcgctgtcc caccccgtaa gcgctgccct tatggcccag ccgtcgctgc
      721 atcgcaagac aactcgatgt tctaggacca tttgcagcaa cggatttgtc gccgtgttgc
      781 accggtcgaa tttcggttac tgggcggacg gacgtgccaa acgccaagca taagcggcaa
      841 cagcaccgca aaagagccct gacgcgctgt gaaaagccac ggagatccag cgtccactgg
      901 acgtggcggc gatcaccgca tccccgaagc aaaccagcgt cgccaggctg aaccacagcg
      961 cgacagcgcg ccagtcccga agcactgcga atgcaatcat caacaggccg agccacaagt
     1021 cgcgtaacgc gatggcggcg tgaggcgcaa acccagggtt gtggcggtcg atgccgaaga
     1081 acgtcgctgc gtgttcgggt tgcagcagaa accgagcgcc gatcaggctg agaacgactc
     1141 cagtggcaat ggcgagccag acgataacgt ccgtaagccg cccttgcccg cgctgtgcca
     1201 tctcaccctc tacgtagcga aactacggcg tcatcccggg ccttgtgcct gggatccgcc
     1261 tatccgcccg ctccggagca agaggcgaaa tacatcccgg cgacaagcgc cgggatgact
     1321 cggggaacct tcgggtccct atcaccggtc gatctcgccg aacacgatgt ccagcgatcc
     1381 gaggatcgcc gacacgtccg ccagcatgtg gccccggttc atgaagtcca tggcctgcag
     1441 gtgggcgaaa ccgggcgcgc gaatcttgca gcgatagggc ttgttgctgc catcagagac
     1501 cagatacacg ccgaactcgc ccttcggcgc ctccaccgcg gcgtagactt cgcccggcgg
     1561 aacgtggatg ccctcagtgt acagcttgaa gtggtggatc agcgcctcca tcgagcgctt
     1621 catctccgcg cgcctgggcg gcacgacctt cgggtcctgg gcgatgtgcg gcccttgccc
     1681 ctcggcggag agcagcttct cgcagcactg tttcatgatc cgcacggact ggcgcatctc
     1741 ctccatgcgg atgagctgcc ggtcgtagtt gtcgccgttc ttgccgacgg gaatgtcgaa
     1801 ctcgaggtca gcgtagcact cgtagggctg ggccttgcgc aggtcccagg cggcgccgga
     1861 gccgcgcagc atcacgccgg tgaagcccca tttcatgcag tcttccagcg agacgacgcc
     1921 gatgtcgacg ttgcgctgct tgaagatgcg gttctccatg acgaggtcgt ggatgtcgtc
     1981 gagaaccttg ggatgcgtct cgcagaactt ggcgatgtcc tcgatcaggt ccggcggaag
     2041 gtccgccttg accccgccga agcggaagta cttggcgtgc atgcgggcac ccgaggcgcg
     2101 ctcgtagaag atcatgagct tctcgcgctc ctcgaagccc cagagcggcg gcgtcagcgc
     2161 gccgacatcc atcgcttgcg tggtgacgtt gagaaggtgg gaaagcaggc ggccgatctc
     2221 gcaatagagc acccggatca gctgcgcgcg gtagggcacc tcgatcccga gcagccgctc
     2281 gaccgccaga cagaacgcat gctcctggtt catcggagcc acgtagtcga gccggtcgaa
     2341 gtagccgatg ttctgtaagt aagtgcgcga ctcgatcagc ttctcggtgc cgcggtgcag
     2401 aaggccgatg tgcgggtcga cccgcgtgac gacctcacca tccagctcca gcacgagccg
     2461 caacacgccg tgcgccgccg ggtgctgcgg accgaagttg atggtgaatg ggcgaatatc
     2521 cgtttctggc atctggctct cgaggcgcta gcttccagcc ttctcgtccc ccggtaataa
     2581 tcgtccggtt ccctcccagg ggctctcgaa atcgaaatca cgaaattcct gcgtcagacg
     2641 gaccggctcg tagacgacgc gcttctgctc atcgtcatag cggacctcga cgtagc
//
LOCUS       DASUMG010000501         2298 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_790711, whole genome shotgun sequence.
ACCESSION   DASUMG010000501 DASUMG010000000
VERSION     DASUMG010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790711"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..1264)
                     /locus_tag="VFX46_08320"
     CDS             complement(305..1264)
                     /locus_tag="VFX46_08320"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600626.1"
                     /translation="MKGIGALPSRGEQRAFGKVRAILLAFAMALALVNPLRASAEDVV
                     HEHLGLKLNGRLEIAPGKSLEKDGVALILHGTLAHHGMEIIRDLQANLKQRGVNTLAV
                     TLSLGVDGRTGMFDCAQEHAHRHSDAVDELSSWLEWLEARKAAWVDLIGHSRGGAQVA
                     LFAAEQANRISGRIVLVAPLLENTSAEEQASRYEAQFGKPLEPLLAEARTRVERGQAD
                     SPMDVPGFLTCPNARVHPTTFLDYYAGSRRSVPELLNDIGAPTLAIAAGGDEVVPGIV
                     DALSAAGLPDNVTLTEVDGADHFFRDLFGEDLADEIATFLRRG"
     gene            complement(1586..1936)
                     /locus_tag="VFX46_08325"
     CDS             complement(1586..1936)
                     /locus_tag="VFX46_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600627.1"
                     /translation="MNWVRIWLAGVLLAGASSAAMAFDVQQGGQIPASGESKAAPTEV
                     PLGADLSMSETTSAPSAGTEVRIPGLGKLGVPPKMDFGLELLYGAADPKQPETSPEAP
                     ADGVVVRGTVKHRF"
BASE COUNT          446 a          737 c          689 g          426 t
ORIGIN      
        1 cgaacgcatg agggctttgg gcctggagcc cgagggtagc ccatcggcgt cccagcgccg
       61 ggcagcccgg cgatctggag ccgacttcac gcagcgtgaa tgcgagcaaa tctgggattc
      121 gggcaccggc atgaatcgcc agcaatggtc ggccagctgc cgtcgcgtgg ggcagcgcat
      181 ggagcagctc cgccgcatag gtcgaggggg gacgctgagg gaacggcgct cgccgataag
      241 tccatcggac ataggagtgt tcggctcaca ggctgacccg gacgcccgat gagcgggggc
      301 ctgatcaacc tcgccgcagg aatgttgcga tctcatcggc cagatcctcg ccgaagaggt
      361 cgcggaagaa gtggtccgcg ccgtccacct cggtcagcgt cacgttgtcg ggtagtcctg
      421 cagcgctcag cgcgtccacg atccccggca cgacctcgtc gccacccgcc gcgatggcga
      481 gtgtcggagc cccaatatcg ttcagcagct ccgggacgct acggcgcgag ccggcataat
      541 aatcaagaaa agttgtcggg tgtacccgcg cgttcgggca cgtgaggaaa ccgggcacat
      601 ccatcggcga atcggcctgg cccctctcaa ctcgtgtacg cgcctccgcg aggagaggct
      661 caagcggctt gccgaactgg gcttcgtatc ttgaggcttg ctcctcggcc gaggtgttct
      721 ccagcaaagg ggcaacgagg acaatccgac ccgatattct gttcgcctgc tctgccgcaa
      781 acagtgcaac ttgcgcgccg cctctggagt ggccgatcaa atccacccaa gccgcctttc
      841 gggcctcgag ccattcgagc caactgctca gctcgtcgac cgcatcgctg tggcggtgag
      901 catgctcctg tgcgcagtcg aacatgcctg ttcggccgtc gacgccgagg ctcaaggtca
      961 ccgctaaagt gttgactccc cgctgcttca ggttcgcttg caagtcgcgg atgatttcca
     1021 tcccgtggtg ggcgagggtc ccgtggagga tgagcgcaac gccatccttt tccagagact
     1081 tgccgggcgc gatttccagc cggccgttga gcttgagccc aagatgctcg tggaccacat
     1141 cctccgccga ggcgcgaagc ggattgacga gtgccaacgc catggcgaac gcgaggagga
     1201 tcgcccgaac tttgccaaaa gccctttgct ctccgcggct tggcaaagct ccgatcccct
     1261 tcatctcgcg ctccgtgctc tgaacaggcc caggaaagct catccgagca aagctgccca
     1321 gcgtgcagag cattcgcaag tccatctatc agctaagggg ttagcgccat ctaggcgaac
     1381 cctatgtgaa gcatgcccgc ctgcatgagc atgaggtcag cctgccaaga cctgagaacc
     1441 cgcccatgaa gtagcgcaat tgggctactt ggcgcgacag aacacacgca ctccccgcga
     1501 cgcaccgaca atccacgtcg cagaaggtgc gcaccgacgt caaccgaaag ttgatcctat
     1561 cgcaggctct ctaccaatcc gagtgctaga agcgatgctt gactgtgccg cgcacaacca
     1621 cgccgtccgc tggggcttca ggtgacgtct ctggctgctt ggggtcggcc gccccataaa
     1681 gcagctccag gccgaagtcc atcttcggcg gcacgcccag cttgccaagg cctggaatcc
     1741 gcacctcggt ccccgctgaa ggagcactag tagtttcgga cattgacaga tcggcaccca
     1801 gtggcacctc tgtcggagca gccttcgact ccccgcttgc tgggatctgg ccaccctgtt
     1861 gtacgtcgaa cgccatggct gcgctgctgg ctcccgccaa cagtacaccc gcgagccata
     1921 tacgaaccca gttcattcgg gactccgccg gtccaaatca tccacgcgtt ctgcccggca
     1981 acttgagccg atgtacgcaa gagcgcaaga gtgaatgtaa taaagttaag ggattatgta
     2041 cgtttggctg cgtggtgtgg tttgggaaca atactcgtgg gcgattgccc cctatccacc
     2101 tctgcggtga gctctgtccg gcggacctgt aacccgcgga gcgagcacag cagaaagccg
     2161 cccgggggcg gctttcctca acttctctgt gttcgccgtt tagaagcgaa tcatcgcgcc
     2221 ggcgaagact gcgtggaagt cgtcgaagtc ggctttgcgt ctgaaaacgt cggcgatcgc
     2281 ttcattctct agatctac
//
LOCUS       DASUMG010000502         2599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_229703, whole genome shotgun sequence.
ACCESSION   DASUMG010000502 DASUMG010000000
VERSION     DASUMG010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229703"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFX46_08330"
     CDS             <1..449
                     /locus_tag="VFX46_08330"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX5600628.1"
                     /translation="SFPWPPVLLGASVVAARVLQRLLPLPWPGMDDLASRIAGLGLGL
                     AGILLMAWSIITLRRARTTVMPNEAASNLVTDGPFRFRRNPIYLADMLILLGIAELMR
                     NIWLVLLTPVFGILVTWLAIIPEERHLEARFGDAYRAYKERTRRLI"
     gene            complement(446..892)
                     /locus_tag="VFX46_08335"
     CDS             complement(446..892)
                     /locus_tag="VFX46_08335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX5600629.1"
                     /translation="MSTFEPLDPDFETRVRESFARQAFMRALGAELLQISPGEVHLAL
                     AHKPDLTQQHGYFHGGVIATIADTAAGYAALSLYPKGTGVLTTEFKINLLRPAQGERL
                     VARGRVVKPGRTLTICQSDVYAENGGRESHVATLLLSMICLEGLQD"
     gene            1085..2380
                     /locus_tag="VFX46_08340"
     CDS             1085..2380
                     /locus_tag="VFX46_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600630.1"
                     /translation="MATTYQAAVLVLALTTGAVTLCPPDSLAQASRRDGIGELIAREG
                     QTSDNNGAQPEAIPQDVREGSVEYEQARRLMKAVDAILEDAAKHRAEARKLPSRKDFV
                     VTPIWTETREDRERKIRDLLDAALGIVTDVPVVAVQRRIETLRKNIRDLEDRIVKLKE
                     KQLVAPQDAILPGVLSDTVESLERDIQESQRRIEENRKAIEAAKAEVHQALKASGLDL
                     SAEQVDLLLDSVLSGDLVRLAAVFNSAKLVDRQLAKMLNASGNNMNAARKYFAMHAAL
                     FAMLVHAQDTLIDKIDNNYLPKLDAIMKDIEAARRKTNELLKAENRPDQRRALEANRE
                     SQKLAEEAAKGYRRYLLQQREQIAEARKRTAHDLRIADNTYETVEASFQLRNLMRDSS
                     ASFEAIQKLEAPTFEQIFRNEELRKEFESLTRKLDSPTS"
     gene            complement(2395..>2599)
                     /locus_tag="VFX46_08345"
     CDS             complement(2395..>2599)
                     /locus_tag="VFX46_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600631.1"
                     /translation="YCYLEDLFVRPAERGKGIGRALIEAVYKEADARQCTRTYWATQE
                     FNYRARMLYDQLATKSPFVQYRR"
BASE COUNT          524 a          814 c          785 g          476 t
ORIGIN      
        1 cctccttccc ttggccacca gttttgctcg gcgcgagcgt ggttgccgcc cgggtcttgc
       61 agcgcctgct ccccctgccc tggccaggca tggacgatct cgcatcccgc atcgctggcc
      121 tgggtctggg gctcgccggc attctgctga tggcctggtc gatcatcacg ctgcggcgtg
      181 cccgcaccac cgtcatgccg aacgaggccg ccagcaacct cgtcacggac gggccgttcc
      241 gctttcggcg taacccgatt taccttgccg acatgctcat tctgctcggc atcgccgagc
      301 tgatgcgcaa catatggctg gtactgctca cgccggtgtt cggcatcctt gtcacttggt
      361 tggctatcat cccggaagag cggcacctgg aggcccgttt cggggatgcc tatcgggcgt
      421 acaaggagcg cacgcggcga ctgatctaat cctgaagacc ttcgaggcaa atcatcgaga
      481 gcaacagcgt ggcgacgtga gactcacgcc caccattctc agcgtagaca tcagactggc
      541 agatggtgag tgtccggccc ggcttgacga cccgcccgcg cgcgaccaac cgctcgcctt
      601 gggcggggcg taacagattg attttgaact ccgttgtcag cacgcccgtg ccttttgggt
      661 atagcgacaa cgcggcgtag cccgcagccg tgtcggcgat ggtggctatc acgccaccgt
      721 ggaaatagcc gtgctgctga gtgagatcgg gcttgtgagc aagagcgaga tgcacttctc
      781 ccggagaaat ctgcaaaagc tccgcgccca gcgcgcgcat gaacgcctgc ctggcgaagc
      841 tttcgcgcac gcgcgtctca aagtcgggat cgagaggctc gaacgtgctc atccgttact
      901 ccggtcactt ctagtgaccg agaacatggc acgaaccctt ggcctcccgc cagccactat
      961 ttttggatgg ctccattgca atcaaagccg tccgtgagca cgtaatccca tcagagatga
     1021 gcgttttttc attgattcgc ccgattctgc aggccagtgt gcaacggggg gtggaggtgc
     1081 gatcatggcg acgacgtacc aggctgccgt tcttgtgctg gcactgacaa cgggggcagt
     1141 cacactttgc cctccggact cccttgcaca agcaagccgc cgcgatggga tcggcgagct
     1201 tattgctcgg gaaggtcaaa cttcagacaa caatggtgct cagccggagg ccattcccca
     1261 ggacgtccgg gaaggcagcg tcgaatatga gcaggcgcgg cgcttgatga aggccgtcga
     1321 cgccattctc gaggacgcag ccaagcaccg cgccgaagcc cgaaagctgc cctcacggaa
     1381 ggatttcgtc gtaacgccga tctggacgga gacccgcgag gatcgcgagc gtaagatccg
     1441 tgatctgctc gacgcagcgc tcggcatcgt cacggatgtg cctgtggtcg ccgtgcagcg
     1501 ccgcatcgaa acgctgcgca agaacatccg cgatcttgaa gaccgcatcg tcaagctcaa
     1561 ggagaagcag ctcgtcgctc cgcaggacgc cattctgcct ggtgttctca gtgacaccgt
     1621 cgaaagtctt gagcgagaca tccaggaatc gcagcgccga attgaagaga accgaaaggc
     1681 catcgaggct gcgaaggccg aggtccatca ggcgctcaaa gcgtctggcc ttgatctttc
     1741 ggccgagcag gtcgacctcc tgctcgacag cgtgctttcc ggcgacctcg tacgactagc
     1801 ggccgtgttc aactcggcca agctggtcga tcgtcagctc gcgaagatgc tgaatgcctc
     1861 cggcaacaac atgaatgccg cgcggaagta tttcgccatg cacgcggcgc tcttcgcgat
     1921 gctggtgcac gcgcaggaca cgctgatcga caaaatcgac aacaactatt tgcccaagct
     1981 cgatgccatc atgaaggaca tcgaggcggc ccggcgcaag acgaacgagc tgctaaaggc
     2041 tgagaaccgg cccgatcagc ggcgggccct ggaggccaac cgcgaaagcc agaaacttgc
     2101 ggaagaagcg gcaaaaggct accggcgcta cctcttgcag cagcgagagc agattgcgga
     2161 ggcccgcaag cgcacagccc acgacctccg tatcgccgat aacacctatg agacggttga
     2221 ggcgagcttc cagctccgca acctgatgcg ggacagctcg gcgtccttcg aggccatcca
     2281 gaagctcgaa gcgccgacgt tcgagcagat cttccgcaac gaggagctgc gcaaggagtt
     2341 cgagagcctg acgcgcaagc tcgattcgcc gacgtcgtaa gatccggcaa ggcttcagcg
     2401 cctgtactgg acgaaaggcg acttcgttgc gagctggtcg taaagcatac gggcccggta
     2461 attgaactct tgcgtcgccc agtaggtgcg cgtgcactgg cgggcgtcgg cctccttgta
     2521 gacggcttcg atcagtgccc ggccgattcc ctttccgcgt tccgccggtc gaacgaacag
     2581 gtcctccaga tagcagtac
//
LOCUS       DASUMG010000503         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_550726, whole genome shotgun sequence.
ACCESSION   DASUMG010000503 DASUMG010000000
VERSION     DASUMG010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550726"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..997
                     /locus_tag="VFX46_08350"
     CDS             83..997
                     /locus_tag="VFX46_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002968018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600632.1"
                     /translation="MNLVAFLGAIEIGLVFGLVGLGVFLSFRVLNFPDLTVDGSFPLG
                     AAVSAALIVSGLDPYSATLVAIACGCLAGLVTAFLNIRFGILHLLASILTMIALYSIN
                     LRIMGRPNLAIISEPTVLSAFEGFGLAMLYLKPLFAGVVAIAGVLLVSRFLASEYGLA
                     MRATGANPRMARAQGVETSRATYVGMAMSNGLVALAGALFAQMNGFADVTLGTGTIVV
                     GLAAVIVGEALLGTRSVLIWAAGCVVGSVAYRLAVALALNSDVLGLQASDLNLVTAVL
                     VTIALILPGAQSSIRSMFSTVRWRRAPT"
     gene            994..1788
                     /locus_tag="VFX46_08355"
     CDS             994..1788
                     /locus_tag="VFX46_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020052020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600633.1"
                     /translation="MIRVEGLNVTFHSGTPMETRALRGLDLEVPSGQFVTVIGSNGAG
                     KSTLLNALAGEVVPQSGRVVINDEDVTRWSAPRRSALVARVSQDPLAGTCESLTIEEN
                     LSLAAARGRRRGLRLAIDRYAREEFRARLRSLGLGLENRLGDKVGLLSGGQRQAVSLL
                     MATLRPMEVLLLDEHTAALDPKTADFVLELTNRIVTEQRLTTLMVTHSMRQALDMGER
                     TVMLHEGKVVLDVAGKERAGLDVPDLLAEFARLRGEQLADDSLLLD"
     gene            complement(1813..>2449)
                     /locus_tag="VFX46_08360"
     CDS             complement(1813..>2449)
                     /locus_tag="VFX46_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX5600634.1"
                     /translation="ALEHLRSHGCDPAHIEAYARDRGPLLKLFCQPPAAHVRLKEGDA
                     LDMGGRRWRVLTNDGHADEHASFVSVDEKVVIAGDQILQRISPVVGVFAGEPNADPLT
                     TYLDSLERFRGLAADTLVLPSHGLPFRGLDARLDQLKQHHRRRLELVHEALDRPRTAT
                     ECSQVLFARAMAEGQWFLALAETIAHLHHLVTIGAARREVGNDSVIRFART"
BASE COUNT          402 a          762 c          784 g          501 t
ORIGIN      
        1 agctctctga tgccgctgcc cccgtgataa tgagaactcc gatgataatg agactggact
       61 gctgcaggag ctcgcacttg ccatgaacct ggtcgcgttt ctgggcgcaa tcgagatcgg
      121 gctggtattt ggcctggtcg gccttggcgt gttcctgtcg ttccgggtcc tcaacttccc
      181 agacctgacg gtcgacggca gttttcctct gggggccgca gtcagcgccg cattgatcgt
      241 cagcggcctc gatccttatt ccgcaacgtt ggtcgccatt gcttgcggct gcctcgccgg
      301 gctcgtcaca gcgttcctga atattcgctt cggaattctc catctcctgg cgagcattct
      361 caccatgatc gcgctctatt ccatcaactt gcgcatcatg ggccgcccca acctcgccat
      421 catttccgag ccgaccgtgc tttctgcgtt cgaaggcttc gggctcgcca tgctgtacct
      481 aaagccgctc ttcgcaggcg tggtggcgat tgcgggcgtg cttctcgttt cgcgattctt
      541 ggcttccgaa tacggtcttg cgatgcgggc cactggagcc aatccgcgca tggcgcgggc
      601 gcagggcgta gagacgagcc gagcgactta cgtcggcatg gccatgagca atgggctcgt
      661 tgcgctggcc ggcgcactgt tcgcgcagat gaacgggttc gctgatgtga cgcttgggac
      721 cggaaccatt gttgtcgggc tcgctgctgt catcgtcggt gaagccttgc tcgggacccg
      781 ttccgtcctg atctgggctg cgggctgcgt cgtcggttcg gtcgcctatc gcttggccgt
      841 tgcactggcg ctcaacagcg acgttctcgg gctccaggct tcggatctca acctcgtgac
      901 ggcggtcctc gtgacgatcg cactgatctt accgggagcg cagagcagca tccgttctat
      961 gttctccacc gtccgctgga ggcgtgcgcc cacatgattc gagtggaagg tctcaacgtc
     1021 acgtttcaca gcggaacgcc gatggagaca cgggccctca gagggctcga tctcgaggtc
     1081 cccagcggtc agttcgtgac tgtcatcggc tccaacggtg cgggtaagtc gacgctgctg
     1141 aatgcactgg ccggagaggt cgttccgcaa tcggggcgcg tcgtcatcaa tgacgaggac
     1201 gttacgcgat ggtccgcacc ccgccgaagc gcgctcgtcg cgcgtgtctc tcaagatccg
     1261 ctcgccggca cctgcgagtc actgacgatc gaggaaaacc tgtcattggc tgccgctcgc
     1321 ggccggcgcc gcggcttgcg gctcgccatc gatcgatatg cccgcgagga gttccgggcg
     1381 cggttacgat cgctaggcct cggtctggag aaccgtctcg gcgacaaagt tgggcttctg
     1441 tccggcggcc agcggcaggc cgtcagtctt ctgatggcga cgctgcggcc gatggaggtg
     1501 ctgctgctcg atgagcatac tgcagcgctc gatccgaaga cggctgattt cgtgctggag
     1561 ctcacgaacc ggatcgtcac agagcagcgg ctgaccacgc tcatggtcac ccattccatg
     1621 cgccaggcgc tcgatatggg tgagcgcaca gtgatgctgc acgaagggaa agtggtcctg
     1681 gacgtcgccg gcaaagagcg ggcaggtctc gatgtgccgg acctcctggc cgagttcgcc
     1741 aggttgcgcg gcgagcaact cgcagacgac agcttgctgt tggactaacc cgccttcgtt
     1801 tcggtgaagt tactacgtcc gggcgaagcg gatcacgcta tcattgccca cctcacggcg
     1861 tgctgccccg attgtgacga ggtggtgcaa gtgagcgatc gtctcggcga gcgccagaaa
     1921 ccactgaccc tctgccatgg cccgggcgaa gagcacctgc gagcactccg tcgccgtgcg
     1981 tggtcggtct agggcttcgt gaacgagctc cagccggcgc cggtggtgct gcttgagctg
     2041 gtcgagccgc gcgtcgaggc cgcggaacgg cagcccgtgc gaaggaagca cgagcgtatc
     2101 ggccgcaaga ccgcgaaacc gttcgaggga atcgagatac gttgtcagcg gatctgcgtt
     2161 cggctcaccg gcgaacacac ctacaaccgg tgagatgcgc tggaggatct gatccccggc
     2221 gatcaccact ttctcgtcga cgctgacgaa ggaggcgtgc tcgtccgcgt gaccatcgtt
     2281 cgtcagcacg cgccatcgcc gaccgcccat atcgagggca tctccctcct tcaggcggac
     2341 gtgggccgcg ggtggctggc agaagagttt cagcaacggt ccgcggtcac gcgcgtatgc
     2401 ttcaatgtgc gcaggatcac agccatgact gcgcagatgt tcgagcgca
//
LOCUS       DASUMG010000504         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1192969, whole genome shotgun sequence.
ACCESSION   DASUMG010000504 DASUMG010000000
VERSION     DASUMG010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192969"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFX46_08365"
     CDS             <1..775
                     /locus_tag="VFX46_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5600635.1"
                     /translation="NKPVGLMVRRLLAHRIRARWRRLQGETLIGLGFATPYLGIFRGE
                     ALRIGAFMPTGQGALVWPDIGPKLSVLVEDERLPLPDNSVDRLLVVHCLENAGRPRQL
                     LREIWRVLTPQGRLMLIVPNRRGVWARLDRTPFGQGRPYSRTQLEELLSESMFSSFDW
                     SAALFVPPIESPVLMRSATAFERIGARLSRGFGGVIIVEAHKELTAPVAKKAPMRVIG
                     RLVTAPGAATPRTKPGNRALSTPTGASAETPLSHQKYSA"
     gene            complement(714..1355)
                     /gene="metW"
                     /locus_tag="VFX46_08370"
     CDS             complement(714..1355)
                     /gene="metW"
                     /locus_tag="VFX46_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEX5600636.1"
                     /translation="MLRSQQPLQTTPRVDHLLIAEMVTENARVLDVGCGDGALLDLLI
                     QTKGVDGRGIEIERDRVNACVARGLSVIQGDADRDLDDYPDDAFDYAILSLTIQATLR
                     PKTVLQNLLRIGRYAIVSFPNFGHWRVRWQLLFTGRMPQTENLPEPWYESPNAHLCTI
                     RDFADLCAKVDATVERSAAFNSSGRQLSPRAPLIMQNIFGEKAVFLLRRRSAY"
     gene            complement(1378..>2395)
                     /locus_tag="VFX46_08375"
     CDS             complement(1378..>2395)
                     /locus_tag="VFX46_08375"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEX5600637.1"
                     /translation="NAILVCHALTGDQHVANRHPVTGKPGWWETMVGPGKPVDTNRFF
                     VICPNIIGGCMGSTGPASIDPSTGKPYGLNFPVITIGDMVDAQVRLIDRLGIDQLFCV
                     IGGSMGGMQVLEWASRYSERVFAAVPIATAALHSAQNIAFHEVGRQAVMADPDWCGGR
                     YLEFGRVPRRGLAVARMAAHITYLSEEALHRKFGRNLQDRTDRSFSFSADFQVESYLR
                     HQGISFVDRFDANSYLYITRAMDYFDLAAEHGDVLANAFRGTKTRFCVVSFTSDWLYP
                     TRESKQIVQALNAVAANVSFVEIESDKGHDAFLLDEPELFATVRGFINSAAAKRGIPP
                     ARHG"
BASE COUNT          453 a          759 c          744 g          439 t
ORIGIN      
        1 taacaagccg gtcggcctaa tggttcggag gctgctggcc catcgtattc gggcccgttg
       61 gcggcggttg caaggagaga cgctgattgg actcggcttt gcaactccct atctcggcat
      121 ctttcgcggt gaggcgctgc gaatcggcgc tttcatgcct acaggccagg gagcactggt
      181 ctggcccgat atcggaccga agctgagcgt cctcgtggag gatgagcggc ttccgctgcc
      241 cgacaactcc gtggacaggt tgctcgtcgt tcactgcctg gaaaatgccg gccggccgcg
      301 gcagctcctg cgggagatct ggcgtgtact cactccgcag gggcgactga tgctgatcgt
      361 gcccaaccgc aggggtgttt gggctcggct cgaccgcaca ccctttggtc aaggccggcc
      421 ttacagccgc acccagctcg aagagctcct gagtgagtcg atgttcagct cgttcgactg
      481 gtcggccgcc cttttcgtgc cgccaatcga gtcgcccgtt ttgatgaggt ctgcaacggc
      541 cttcgagcgt ataggcgcgc gactgtctcg tgggtttggt ggcgtcatta tcgtggaggc
      601 acacaaagaa ctgaccgcac cggtcgcgaa gaaagcaccc atgcgtgtca tcggccgcct
      661 cgtcaccgcc cctggcgcag ccacaccacg cactaagcct ggcaatcgtg ccctcagtac
      721 gccgaccggc gcctcagcag aaacaccgct ttctcaccaa aaatattctg cataatgagc
      781 ggcgcgcgcg gcgagagctg ccggcctgac gaattgaacg cggctgaacg ctcgaccgtc
      841 gcatccacct tggcacacag gtccgcgaaa tcccggatcg tgcacaagtg cgcgttgggc
      901 gactcgtacc aaggctccgg aaggttctcc gtctgcggca tccggccggt gaacagaagc
      961 tgccaccgca ctcgccaatg gccgaagttc ggaaatgaga cgatcgcgta tctgccgata
     1021 cggagcagat tctgcagaac cgtcttcggc ctgagcgtcg cctgaattgt gaggctcagg
     1081 atggcatagt cgaatgcgtc gtccgggtag tcgtccaaat cgcggtctgc atcgccttgg
     1141 atgacggaca acccgcgcgc gacgcaggca ttcacgcggt cgcgctcgat ctcgatgcca
     1201 cggccatcga cccccttggt ctggatcaac agatccagca acgcgccgtc gccacaaccg
     1261 acatcgagta cgcgtgcgtt ctcggtcacc atctcggcaa tgaggagatg gtcgacacgg
     1321 ggcgttgttt gcaaaggctg ttgcgaccga agcatcagta ttcctacagc gcagcactta
     1381 accgtggcga gcgggcggaa tgccccgctt ggccgccgcg gaattgataa accctcggac
     1441 ggtggcgaac agctcgggct cgtcgagcaa aaaggcgtcg tgccctttat cgctctcgat
     1501 ttccacgaac gatacgttgg cggcgacggc gttcagcgcc tgcacgatct gcttcgactc
     1561 gcgtgtcgga tagagccagt cgctggtgaa ggacacgacg cagaaccgcg tcttcgtccc
     1621 tctgaaggcg ttcgccagca cgtcgccatg ctccgcggcc aggtcgaagt agtccatggc
     1681 ccgcgtaatg tagagatagc tattggcatc gaaacgatca acgaaactga tgccctgatg
     1741 cctcagatag ctctccacct ggaagtcggc gctgaaggag aatgaacggt ccgtccggtc
     1801 ttgcaggttg cggccgaact tccggtgcag ggcctcctcc gagaggtagg tgatgtgcgc
     1861 tgccatccgc gccaccgcca gcccgcgccg cggcacgcgc ccgaactcga gataccggcc
     1921 gccgcaccag tcaggatcgg ccatcacggc ctgccggccc acctcgtgga aggcgatatt
     1981 ctgagcggag tgcagcgctg ccgtcgcgat cggcacggcc gcaaagacgc gctcgctata
     2041 gcgggatgcc cactccaaca cctgcatgcc gcccatcgag ccgccgatca cacagaagag
     2101 ctggtcgatg ccaaggcggt cgatcaggcg cacctgggcg tcgaccatgt cgccaatggt
     2161 gatgaccgga aagttcaggc catacggctt gccggttgaa ggatcgatgg acgcaggccc
     2221 ggtggagccc atgcagccgc cgatgatatt ggggcaaatg acgaagaaac ggttcgtgtc
     2281 gaccggcttg cccgggccga ccatcgtctc ccaccagccc ggcttcccgg tgacggggtg
     2341 tcgatttgcc acgtgctgat cgccggtcag cgcgtggcac accaggatgg cgttg
//
LOCUS       DASUMG010000505         3775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1193007, whole genome shotgun sequence.
ACCESSION   DASUMG010000505 DASUMG010000000
VERSION     DASUMG010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193007"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..2578)
                     /locus_tag="VFX46_08380"
     CDS             complement(23..2578)
                     /locus_tag="VFX46_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEX5600638.1"
                     /translation="MTRKRPTNRSTTGARTDPLLPERFEQWFRSRGWSIRPHQRAMLE
                     KARARRSTLLIAPTGAGKTLAGFLPSLVELVSGERPRRGAGLHTLYISPLKALAVDVA
                     RNLLTPVEEMQLPLRIETRTADTSVGKRQRQRVRPPDMLLTTPEQLALLLSHADAAYL
                     FADLRTIILDELHALVGSKRGDLLALDLARLGTLAPDAIRIGLSATVARPSELRAFLL
                     PQREPDRAIHLADLVIVTGGAKPDIRLLESKNPVPWAGHTTLYALPDVYAAIKAHRMS
                     LLFVNTRFQAELLFHELWRINDDNLPIALHHGSLDATQRRKVEAAMAAGRLRAVVATS
                     TLDLGIDWGDVDLVINVGAPKGASRLIQRIGRSNHRLDEPSKALLVPSNRFEVLECHA
                     ALDAALEGVQDVVLSRKGALDVLAQHVLGTAAAGPFHPEELYKEVISAQPYADLSRRD
                     FDRVVDFVATGGYSLRTYERFARLRPMDDGRLRIAHPRIAQQYRMNAGTIVDAPMLKV
                     RLVRRRGAPAPGHAARLTGGRVLGEIDETFIAELQVGDTFVFGGEIVRFEGLRDTDVF
                     VSRTWDSEPKIPSYPGGKFPLSTHLASRVRNMLADPRAWSSLPEPVAEWLEIQQRRSI
                     IPGSDEVLVETFPRGKRHHLVIYAFEGRLAHQTLGMLLTRRLERARALPLGFVANDYG
                     LAVWTAANLSEMIATGALDLARLFDEDMLGDDLEAWLAESNLMKRTFRVCAIIAGLIE
                     RRHPGREKTGRQLTISSDLIYDVLRRHDPGHVLLEAAWADAATGLLDIARLGDFLRRI
                     KGRIRHHSLASVSPLAVPILLEIGREPVHGAAREALLRDAADELIAEAVGEGT"
     gene            2715..2990
                     /locus_tag="VFX46_08385"
     CDS             2715..2990
                     /locus_tag="VFX46_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HEX5600639.1"
                     /translation="MQHNRFFGGNPVSVLVRLALISIVVGVVMSALDIRPDNILLFLD
                     RTLQRIWEMGFGSIEWLLEHLLLGAMVVIPIWLIARLIGMGRSGRET"
     gene            3009..>3775
                     /locus_tag="VFX46_08390"
     CDS             3009..>3775
                     /locus_tag="VFX46_08390"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX5600640.1"
                     /translation="MQLKDSGERQPISARPIGHRDVVAIALPIMLSNATVPLIGFVDT
                     VVVGQLGQAHLIGAVAVGATIFNFLYWGFGFLRMGTTGLTAQALGAQDTAEVAANLYR
                     ALLIAITAGLAMIAAQAVIAWAAFQIMGASAAVENAARLYFEIRIWAAPAGLINFALL
                     GWFIGLGRAGLAFAIQIFLNIVNVLLAVLFVIGLGYGVLGVGLAALLAEWLAALFGLM
                     LARADLVRRGASAPLRQVLDVGRMKRTFQVNTDIMIRT"
BASE COUNT          638 a         1171 c         1286 g          680 t
ORIGIN      
        1 cgctccggct tcaacgcgag cattatgtcc cttccccgac agcctcggcg atgagttcat
       61 cagctgcgtc gcgcagcaag gcctcgcgtg ccgcaccatg aaccggctcg cgcccgattt
      121 ccagaaggat gggcacagcc agtggcgaca cgcttgccaa ggagtggtgt ctgattcgtc
      181 cttttatgcg gcgaagaaaa tcaccgaggc gggcgatgtc caacagtccg gttgccgcat
      241 cggcccaagc cgcttcgagc agcacatggc ccgggtcatg ccggcgcagg acatcgtaga
      301 tgaggtcgga cgagatggtg agctgccggc ctgtcttttc tcgacccgga tggcggcgct
      361 cgatcagtcc tgcgatgatc gcgcagaccc ggaaggtgcg cttcatgagg ttcgactcag
      421 cgagccatgc ctccaggtcg tcccccagca tgtcctcatc gaacaggcgc gccagatcga
      481 gagcgccggt ggcgatcatt tcggagagat tggcagcggt ccagacagcg aggccgtagt
      541 cgttggccac gaacccaagg ggcagagcgc gggcgcgctc gaggcgccgg gtcagcagca
      601 tgcccagcgt ctgatgggcg aggcggccct cgaacgcgta gatcacgagg tgatgccgtt
      661 tgccgcgagg gaaggtctca accagcacct catcagagcc cggaatgatt gagcggcgct
      721 gctggatttc gagccactcg gcaacgggct cggggaggga cgaccacgcg cggggatcgg
      781 ccagcatgtt tcgcacgcgg gatgcaagat gggttgagag cgggaacttg ccgccgggat
      841 agctcggaat tttcggctcg ctgtcccagg tgcgcgagac gaatacgtcc gtgtcgcgca
      901 ggccttcgaa acggacgatt tcgccgccaa acacgaacgt gtcaccgacc tgcagctcgg
      961 cgataaacgt ctcatcaatt tcgccgagca cccgcccgcc ggtgagccgc gctgcatgcc
     1021 caggcgccgg ggcgccgcga cgccgaacca gacgcacctt cagcatgggc gcatcgacga
     1081 tcgtgccggc attcatccgg tactgctggg caatgcgcgg gtgggctatg cggaggcggc
     1141 catcatccat cggccggagg cgcgcgaagc gctcataggt tcggagcgag tagccgccgg
     1201 tggcaacgaa atcgaccacg cgatcgaaat cccggcggct gaggtctgca tagggctgag
     1261 cggagatcac ttccttgtaa agctcctccg gatggaatgg gcccgcagcc gccgtcccca
     1321 gcacgtgctg ggcgagcaca tccagtgctc ctttccggga gagcaccaca tcctgaacgc
     1381 cttcgagcgc cgcatcgaga gctgcgtggc attcgagcac ctcgaagcgg ttcgacggca
     1441 ccagcagcgc cttcgagggc tcgtccagac ggtgattgga gcgaccgata cgctggatga
     1501 gccggctcgc ccccttcggc gcaccgacat tgatgacgag gtcgacatcg ccccaatcga
     1561 tgccgagatc gagcgtcgag gtcgccacca cggcgcgcag ccggcccgcg gccatggccg
     1621 cctcgacctt gcgccgctgc gtggcatcga gtgagccgtg gtgcagcgcg atcggcaaat
     1681 tgtcgtcgtt gatccgccac agctcgtgga aaaggagctc cgcctgaaag cgcgtgttca
     1741 cgaacagcag cgacatccgg tgcgccttga tggccgcata gacgtcgggg agggcgtaaa
     1801 gcgtcgtatg cccagcccaa ggcaccgggt tcttgctttc aagcagccga atgtccggct
     1861 ttgccccacc ggtgacgata actaggtcgg cgaggtggat ggcccggtcg ggctcacgct
     1921 gcggcaggag gaaggctctc agctcggatg ggcgtgcgac ggtggcggaa agaccgatgc
     1981 ggatcgcgtc cggcgccagg gtgccgagtc gggcgagatc gagcgccagc aggtcaccgc
     2041 gtttcgagcc gacgagcgca tgcagctcat cgaggatgat cgtgcgcaag tccgcgaaga
     2101 ggtaagccgc gtccgcgtgg ctcagcagca aggcgagctg ctccggcgtc gtaagcagca
     2161 tatcgggcgg gcgcacccgc tggcgctgcc gtttgccgac cgaggtgtcc gctgttcgcg
     2221 tctcgatgcg gagggggagc tgcatctcct cgaccggcgt gagcaggttg cgcgcaacgt
     2281 cgaccgcgag cgccttgagc ggagaaatat aaagtgtgtg caggcccgca ccccgccggg
     2341 gtcgttcgcc ggaaaccagc tcgacgaggc ttgggaggaa gccggcaagt gtcttgcccg
     2401 cgccggtcgg agcaatgagc agcgtcgagc gccgcgcgcg agctttttcc agcatcgcgc
     2461 gctgatgcgg ccggatggac cacccgcgcg accggaacca ctgctcaaag cgctccggca
     2521 gcagtggatc ggtgcgtgct cccgttgtgg aacggttggt cggccgtttg cgggtcatgt
     2581 ggctcgcagg cgcggagatg cgggtgagct tccgctcgaa ggttcagtcc tggtgcgatc
     2641 tatgctagag gctcgggtcg cgcggagaga agcgcctctc cctgcggcgc ggcggctcag
     2701 caaggctgga gaacatgcaa cataatcggt ttttcggcgg caatcccgtc agcgtgctcg
     2761 tccgcttggc gctgatctcc atcgtcgtcg gcgtcgtgat gtcggcgctc gacattcggc
     2821 cggacaacat cctgctcttc ctcgatcgga cgttgcagcg catctgggag atgggcttcg
     2881 gctccattga gtggctgctc gagcacctcc tgctcggtgc aatggttgta attccgatct
     2941 ggctcattgc gcggctcatc ggcatgggcc gttccggcag ggagacctga ggcacatcgg
     3001 tctccgacat gcagctcaag gattccggag agcgccagcc catcagcgcg cggccgattg
     3061 gtcatcgcga cgtcgtggcc atcgcgctgc ccatcatgct ttccaatgcc acggtgccgc
     3121 tcatcggctt cgtcgacact gtcgtcgtcg ggcagctcgg gcaggcgcac ctcattggag
     3181 cggtggcggt cggcgccacg atcttcaatt ttctctactg gggcttcggc ttcttgcgca
     3241 tgggcactac gggcctgacg gcgcaggcgc tgggcgcgca ggacaccgcc gaggtcgcag
     3301 ccaatctgta ccgggcgctg ctcattgcga tcaccgccgg tctcgcgatg atcgcggcgc
     3361 aagcggtcat cgcgtgggct gcatttcaga tcatgggggc ctcggccgca gttgagaatg
     3421 cggcacgcct ttatttcgag atccgaatct gggcggcccc tgcagggctc atcaatttcg
     3481 cgttgctcgg ctggttcatt ggtctgggcc gggccgggct ggcgtttgcg atccagattt
     3541 ttctcaacat cgtaaacgtg cttctggccg tgctcttcgt gatcgggctg gggtacggcg
     3601 tccttggtgt tggcctggcg gcactgctgg ccgagtggct ggctgcgctc tttgggctga
     3661 tgctggcgcg agcagatctc gtgcggcgag gtgccagcgc gccgcttagg caagtgctgg
     3721 atgtcggccg catgaagcgg acgttccagg tcaacaccga catcatgatc cgcac
//
LOCUS       DASUMG010000506         2026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_551388, whole genome shotgun sequence.
ACCESSION   DASUMG010000506 DASUMG010000000
VERSION     DASUMG010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551388"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="VFX46_08395"
     CDS             complement(<1..1355)
                     /locus_tag="VFX46_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX5600641.1"
                     /translation="MLGWAFAAGVVAFVAGSAVAGYLLWYVSRDLPSYESLAEYEPPV
                     MTRIHAHDGQLIAEYGRERRIFVPINAVPKLVIHAFLSAEDKRFYEHGGLDFFGIARA
                     VIRNLQNPGRRPEGASTITQQVAKNFLLSSEQNYERKLKEAILAIRIERAFSKDKILE
                     LYLNEIYLGIGSYGVAAAALNYFNKELEDLTPEEAAYLAALPKAPNNYHPFRRTREAT
                     IRRNWVLDQMVENGYLSREEAEEAKAKPLQVNLRPFGAHIFAAEFFAEEVRRTLLARF
                     GEDKLYGGGLSVRTTLDPELQRHARKALIDGLVEFDRSRGWRGPVDRIDVSGDWGAKL
                     AEMSTPSDIDPWRLGVVLKVESAKIIVGLRPMREPDGSISPKREAVEISFEEMKWAGK
                     FRATDRPKGPAPKKPSDVVEVGDVVYVSPKDPNEPTGAWSLMQIPEVGGGIVAMDPHT
                     GR"
     gene            complement(1621..>2026)
                     /locus_tag="VFX46_08400"
     CDS             complement(1621..>2026)
                     /locus_tag="VFX46_08400"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX5600642.1"
                     /translation="SNEQARRLAEKENSVDLLAGLAPASLEAQDEVRNILIDLLRRET
                     ETFSAEFSNLLTNELRKHMKLARDPQRSAAFLVLQQTESPSVLVELGYMSNEEDQKLL
                     KSPEWQTNIARAILAAVDAFFARRAMGAVHVP"
BASE COUNT          376 a          624 c          618 g          408 t
ORIGIN      
        1 cggcccgtgt gcgggtccat cgccacgatg ccgccgccga cctccgggat ctgcatcaag
       61 ctccaggccc cggtaggctc gttcggatcc ttgggcgaga cgtagaccac gtcccctacc
      121 tccacgacgt ccgacggctt cttcggcgcc ggtcctttcg gacgatcggt ggcgcggaac
      181 ttaccggccc acttcatctc ctcgaacgag atttctacgg cttcccgctt gggagagatg
      241 ctgccgtccg gctcgcgcat cggcctgagg ccaacaataa tcttggccga ttccactttg
      301 aggacgacgc ccagccgcca ggggtcgatg tcggagggcg tgctcatttc ggccagcttc
      361 gcgccccagt cgccgctcac atcgatacgg tcgacggggc cgcgccagcc gcgagagcga
      421 tcaaactcga cgaggccgtc gatcaaagct tttcgtgcgt gccgctgcag ctcgggatcg
      481 agtgtggtgc gcaccgaaag tccaccaccg tagagtttgt cctcgccaaa ccgggcgagc
      541 aacgtccggc gcacctcctc ggcgaagaac tcggcagcaa agatgtgtgc tccaaagggg
      601 cgcaggttca cttgcaaagg cttggccttg gcctcctcgg cctcctcgcg gctgagatag
      661 ccgttctcga ccatctgatc gagcacccag ttccgccgta tggtggcttc gcgggttctg
      721 cggaagggat ggtagttgtt gggagccttc ggcagcgccg ccaagtaggc ggcctcctcc
      781 ggcgtaagat cctccagctc cttgttgaag tagttgagcg cggctgccgc tacgccatag
      841 gagccgatgc cgaggtagat ctcgttgagg taaagctcga ggatcttgtc cttcgagaaa
      901 gcccgctcga tgcggatggc gaggatcgcc tccttcagct tgcgttcgta gttctgctcg
      961 ctggaaagaa ggaagttctt ggcgacctgc tgggtgatgg tcgacgcacc ttccgggcgt
     1021 cgccccggat tctgcaagtt cctgatgaca gcgcgcgcga tgccgaagaa gtcgagcccg
     1081 ccatgctcgt agaatcgctt gtcctcggct gatagaaacg catggatcac gagcttcggc
     1141 acggcgttga tcggcacgaa gatgcgccgc tcgcgtccat actcggcgat gagctgcccg
     1201 tcgtgcgcat ggatgcgggt catcacgggc ggctcgtatt cggccaagct ctcgtagctc
     1261 ggcaggtccc gcgagacgta ccagagcaaa tatccggcaa cggccgatcc cgccacgaaa
     1321 gccaccacgc cggccgcgaa cgcccagccc agcacgctca agaggatgct cttccggcgc
     1381 tttcgtcttc gcttcggcgc gacaggcggg ggcagtattg gtgctcgcat gggccttcag
     1441 cttctaggat cggttgcggc ttcctcacac agccaaacgg caacaaaacg gcatgggaac
     1501 ttagctagca cggttcggct agcttgttcc taaaaatggg cagttcggcc caagaatttc
     1561 acaggccggt gtcggattaa ctggacaaat gtgagtatta taagagattc acctagaata
     1621 tcacggaacg tggaccgcgc ccatagctcg tcttgcgaag aatgcgtcga ctgctgcaag
     1681 gatcgcgcgg gcgatgttgg tctgccactc cggcgacttc agaagcttct gatcctcctc
     1741 gttgctcata taccccagct cgacgagcac ggaaggggat tcggtttgtt gcaggaccaa
     1801 aaatgcagcc gaccgctgag gatcgcgggc cagcttcatg tgcttacgca actcgttcgt
     1861 gaggagattg ctgaactctg ctgagaaggt ctccgtctcc cgcctaagca ggtcgatcaa
     1921 aatgttgcgc acctcgtcct gagcttcgag cgaggcgggc gcgaggccgg ctaggagatc
     1981 gacagagttc tccttctccg caagtcggcg tgcctgctcg ttcgag
//
LOCUS       DASUMG010000507         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_469901, whole genome shotgun sequence.
ACCESSION   DASUMG010000507 DASUMG010000000
VERSION     DASUMG010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469901"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFX46_08405"
     CDS             complement(<1..373)
                     /locus_tag="VFX46_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5602 domain-containing protein"
                     /protein_id="HEX5600643.1"
                     /translation="MTLAGVSPSFAHEPDKALAKSPPPAPYQQVSKLVKLPDFIPGLG
                     QLFVDPATLPAGPFLAYDRDGNLVSTIYMIPLNELKPDGKAFNDLKLSGAAVDHVDVH
                     YNAGHPGVETPHAHIVLWHVKP"
     gene            complement(621..1379)
                     /locus_tag="VFX46_08410"
     CDS             complement(621..1379)
                     /locus_tag="VFX46_08410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX5600644.1"
                     /translation="MSTAGKVAVVTGAGSGIGRAASLALQRNGWHVVLAGRRKEELER
                     TAAMATQGGGEMLVVPTDITNPDSVRALFQATKDKFGRLDLLFNNAGTGAPAIPMEDL
                     TLEQWQTVVNVNLTGSFLCAQEAIRIMKSQTPKGGRIINNGSISAHSPRPNSAPYTAT
                     KHAITGLTKSISLDGRKHDIACCQIDIGNADTPMGGRMKHGVPQADGTIAPEPVMDVE
                     HVGNAIAYMASLPLDANVQFMTIMATKMPFIGRG"
     gene            1564..1956
                     /locus_tag="VFX46_08415"
     CDS             1564..1956
                     /locus_tag="VFX46_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600645.1"
                     /translation="MILVMHTTEGSPMASSKLLVLAAFTASCVIAGSAWAGGKRWDRS
                     PHYGKYEGYVVAESRFGHGTVVGPVRRGRTGWEVRMPGGTWIACRRSCSETLRVETVD
                     FWENRGAGPGASDTECGILGCLRWEWEY"
BASE COUNT          410 a          729 c          751 g          521 t
ORIGIN      
        1 caggcttcac gtgccagagc acgatgtgcg cgtgcggcgt ctcaacaccc gggtggccgg
       61 cgttgtagtg cacgtccacg tggtcgactg cggcgccgga taatttgagg tcattgaagg
      121 ccttgccgtc gggtttcaac tcgttcagcg ggatcatgta gatcgtgctg accaggttgc
      181 catcgcggtc gtaggccaag aacgggcccg cgggcaacgt cgctggatca acgaaaagct
      241 ggcccaggcc cgggatgaaa tccggcagct tcaccagctt gctcacttgc tggtagggcg
      301 caggaggtgg cgatttggcc agtgccttgt cgggttcatg agcgaacgag ggagacaccc
      361 ccgccagggt catcgctgct gacgcgagca gaagcgtgga gagtcgcatt ttgctgtact
      421 ccgatcatga tccaatgcga cgcccttact ggcgtccttc cattggatgc tgcttcggag
      481 ccgaggttcc cggtttttgc gaagccggtt tctcggggag agacgggcgt ccagtgactg
      541 cggtctcagc caaaaaatcc ggccaccaga gtatcatctg gtggccgggc caatcgttca
      601 gacttgaaga gcttgggcgt tcaaccccgc ccgatgaacg gcatcttcgt cgccataatg
      661 gtcatgaact gaacgttcgc atccagcggc aagctggcca tgtaggcgat tgcgttgccg
      721 acgtgctcca cgtccatcac gggctccggc gcaatggtcc catcggcctg cggaacaccg
      781 tgcttcatcc ggccgcccat cggcgtgtcg gcgttgccga tgtcgatctg gcagcacgcg
      841 atgtcatgct tccggccatc cagcgagatc gacttcgtca agcccgtgat cgcgtgcttc
      901 gtggccgtgt agggagcgga attcgggcgc ggcgagtggg ctgagatcga gccgttgttg
      961 atgatccgcc cacccttcgg cgtttgcgat ttcataatcc ggatcgcctc ctgggcgcac
     1021 aggaacgatc cggttagatt cacattcacg accgtttgcc actgctccag cgtcaggtcc
     1081 tccatcggaa tcgctggcgc gccggtgcct gcattgttga agagcaggtc caaccgcccg
     1141 aacttgtcct tcgtggcctg gaaaagagca cgaaccgaat ccgggttcgt gatatccgta
     1201 ggcacaacca gcatctcgcc accgccttgg gtggccattg ctgccgtgcg ctcgagctcc
     1261 tccttgcgcc gtcctgccag cacaacgtgc cagccgttcc tttgcagggc taaagacgct
     1321 gcccgcccaa tgccactgcc cgcgccggtg acgacggcaa ccttgcctgc ggtactcata
     1381 actggtgttt cctccttaga cagcccggcg ccatcctagc gccttcagcg agattgccta
     1441 gagttgcgct gccgcctgcg ctgagatgcg caaatgccat gcatcagcat caattcagcg
     1501 aagcgtgatg tttggccttg gtgccgtctg aacgatacgc taaccggatg tgtggtatcg
     1561 agcatgatct tggtcatgca caccactgag ggtagtccga tggcgtcttc gaagcttctg
     1621 gttcttgcag cgtttactgc cagctgcgtc attgccggat cagcgtgggc gggcggcaaa
     1681 cgctgggatc ggtcgcccca ctacggcaag tatgagggct acgtggtcgc ggaaagccgg
     1741 tttggccacg gcaccgttgt gggacctgtg cgtcgcggcc ggacgggatg ggaggtccgc
     1801 atgcccggcg gcacatggat cgcctgccgg cggagctgct ccgagacgct gcgcgtcgaa
     1861 accgtggact tttgggagaa ccgcggtgcc ggcccaggag ccagcgatac ggagtgcgga
     1921 atccttggct gcctgagatg ggagtgggag tactgatccc cactcaaggg gccgctgcct
     1981 agcagcgcct ctcattgacc ttctgatttt ccaagcttgt cagctcgcgg cggcgaaccg
     2041 ccgctcgacg tgtgggtagc ttttgctccc gaaccggcgt agtgattgca tctcttcgca
     2101 cctcttacct ttctttccga gatgattggg acccctgtca ggggctgaag agcgcttgtg
     2161 aatctctttg ggtcgagaac tgcggagaat gtagccgtct actgcggtgc agccctcttc
     2221 ggctacgttg cgtacctgct agctgtaccg ctcccttgga tgatcgggcc aatggttttt
     2281 tcggctggcc tcgcgctcct ctggaggagc gctcgcgttc cgaggatcac ccgcccgatc
     2341 ggtcagatgg tcgtagcgag tgtcgtcggc ctctccttca cagctgaatc ggtgaccgcc
     2401 ctcggccaac t
//
LOCUS       DASUMG010000508         3486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_711194, whole genome shotgun sequence.
ACCESSION   DASUMG010000508 DASUMG010000000
VERSION     DASUMG010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_711194"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VFX46_08420"
     CDS             <1..441
                     /locus_tag="VFX46_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX5600646.1"
                     /translation="DSRIAPELAFDQGPGDLFVVRVAGNFVTTEGLASLEFGAAVLGT
                     KLIMVLGHSSCGAVNATVAALQKGNDLPGHIADLVRAMKPGIEEEMKKAGPDLEQRAV
                     VANVRHNVKRLEQATPILAEMVAKKKLRVVGAVYDLPTGKVALV"
     gene            638..841
                     /locus_tag="VFX46_08425"
     CDS             638..841
                     /locus_tag="VFX46_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600647.1"
                     /translation="MRYFFHILHGPKVFPDEDGNRLSSPELAIRQAKALAVELSKAGE
                     FCRSNLVLVLDENGNSIFGCRAA"
     gene            977..2029
                     /locus_tag="VFX46_08430"
     CDS             977..2029
                     /locus_tag="VFX46_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5600648.1"
                     /translation="METAPSNPSANSGREAVLVDLALQGGGSHGAFTWGVLDRLLQEP
                     WLRIDGISGTSAGAMNAVVLVDGYENGGPEGARAALEEFWRRVSHAARFSPIRRSPMD
                     VLLGRWTLDSSPFFVAFDLAARVFSPYDLNPRGANPLREILAECVDFERAAKSSIRLF
                     VTATNVRTGRGRVFRNNELTPDVLLASACLPTLFQAIEIDGEPYWDGGYSGNPTITPL
                     VRECTSCDTLLVAVNPVERQGTPRSAREILDRLNEVSFNATLLKELRMIALLRQVADA
                     GTGEGAQWAGMRMHLISSETIDKLGASSKFNAEWDFLTMLMVEGRRCAESFLAAHGGN
                     VGKHSTLNLDALLEQV"
     gene            2034..>3486
                     /locus_tag="VFX46_08435"
     CDS             2034..>3486
                     /locus_tag="VFX46_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEX5600649.1"
                     /translation="MGLLGILLALGLLVWLAYRGWSVLLLAPAAAIIAAAVSREPLLA
                     HWTQTFMGSAAQFLAQFFPLFLLGALFGKLMDDSGSVEAIARFMTERLGARRAILAVV
                     LAGALVTYGGVSLFVAFFVLAPMAQALFQAADIPRRLMPAAIALGTSTFTMTAFPGTP
                     AIQNAIPMPFFGTTPFAAPGLGIIGSAIMLGFGLWWLARSEAAARRAGEGHGGDTDIS
                     PRSVADDQIIRERATTAREFDPAEIGHGRHGETPVPIGIAVLPLVVVVSVNLLMSLVI
                     LPRLDFSFLAEAHWGATSLSAVAGVWSVAVALATAILVLVLFNGARLPALRESMDAGA
                     NASVLPAFSVASLVGFGAVVAMLPAFAAVRDWVLSIGGGPLVSLAVATNILAALTGSA
                     SGGMTIALDALGDTYMRIAAELGIDPALMHRVAAIGSGTLDSLPHNGAVVTLLAVCGS
                     THRESYLDIVMVGIVSALIALVAVIALGSMFGSF"
BASE COUNT          568 a         1014 c         1128 g          776 t
ORIGIN      
        1 gattcccgga tcgcacccga gctggccttt gatcaaggac caggggattt gtttgtcgtt
       61 cgcgttgcag gcaatttcgt aacgaccgag gggcttgcga gtttggaatt tggcgcggca
      121 gtgcttggca ccaagctgat catggtgctc ggccacagca gttgtggcgc ggtcaatgcc
      181 accgtagcgg cccttcaaaa aggcaacgac ttgcccgggc acatcgcgga tctggtgcgg
      241 gccatgaaac cgggtattga agaggaaatg aagaaagcgg gtccggatct tgagcagcgc
      301 gcggttgtcg cgaatgtacg ccataatgtg aagcgactgg agcaggcaac gccgattctg
      361 gcggagatgg tggccaagaa aaagctccgc gtggtcggcg ccgtatatga tcttcccaca
      421 gggaaggtcg ccttggttta gcttcggaca ccgaacgagt tgaatgaatg cgccgcgccg
      481 gtcactgcaa aatcagcggc ggccggccag tgtgacgctc tgcaacaggt cgccgcagtc
      541 tcgcgctcga aaggcggaat cggcaacgaa gatagataca tttgattctt acttacttgt
      601 cactggacac ccgcgtcgtc tgccctattg gaaggcgatg cgatacttct tccatatcct
      661 tcacggcccg aaggtgtttc ccgatgagga cggaaacaga ctttcgagcc ctgaactcgc
      721 gatacgtcag gccaaggccc ttgctgtcga attgagcaag gcaggcgagt tctgccggtc
      781 gaatttggtg ctggtgctgg atgagaatgg caacagcatt ttcggatgcc gggccgcatg
      841 acaagagggc cagaaggcgc gtcgactcac atggctcatg gtcatggtta gctggcgaga
      901 gccaagggta aaacccgatg acaatgaggg tttgttccgc taggttttag cacccgcggc
      961 aatccacgag ggtgtgatgg aaactgctcc ttccaatcct tcggcgaact ccggccgtga
     1021 ggccgttctg gtcgatctcg cgctgcaggg tggcggctct cacggcgcct ttacgtgggg
     1081 cgtgctcgat cggctcctcc aggaaccatg gctgcggatt gatggcatct cgggcacctc
     1141 cgcgggtgcg atgaatgctg tggtgttggt cgatggctat gaaaacggcg ggcccgaggg
     1201 agcgcgggct gcgcttgagg agttctggcg gcgcgtctcg catgccgccc gattcagccc
     1261 catccggcgg agtcccatgg atgtgctcct ggggcggtgg acgttggaca gctcgccctt
     1321 cttcgttgct ttcgacctgg ccgcgcgcgt gttctcgcca tacgatctga acccgcgagg
     1381 cgccaatccg ttacgcgaaa tacttgccga gtgcgttgac tttgagcggg cggcgaagtc
     1441 gtcgatccgt ctgttcgtga cggccaccaa cgttcgcacg ggccgcggcc gcgtattccg
     1501 aaacaacgag ctcacgccgg atgtgctgct ggcgtccgcc tgcctgccga cgctgtttca
     1561 ggccatagag atcgacggcg agccctattg ggacggcggc tattcgggaa atccgacgat
     1621 caccccattg gtacgcgaat gcacatcttg cgatacgttg ctcgttgcgg tcaatccggt
     1681 cgaacgccag ggcacaccgc gttcggcgcg cgagatcctc gatcgtctga acgaggtatc
     1741 cttcaacgcc acgctgctga aggagttgcg catgattgcg ctgttgcgcc aggtcgctga
     1801 tgccggtacc ggcgaggggg ctcaatgggc aggcatgcgg atgcatctga tctccagtga
     1861 gaccatcgac aagcttggag cttcgtccaa gttcaatgcg gaatgggact ttctaaccat
     1921 gctgatggtc gagggccgtc gctgcgcgga atccttcctg gcagcgcatg gtgggaatgt
     1981 tggtaagcat tccaccctca atctggacgc tctcctggaa caggtctgat gccatgggcc
     2041 ttctaggcat tctccttgcg ctgggcctgc tcgtctggct ggcttatcgc ggatggagcg
     2101 tgctcctgtt ggcgcctgcg gcggcaatta tcgcggctgc cgtctctcgg gagccgttgc
     2161 ttgcccattg gacgcaaacc ttcatgggca gcgcagcgca gtttctcgcg caattcttcc
     2221 cgctgttcct gctcggcgcg ctgttcggca agctcatgga cgacagcgga tcggtcgaag
     2281 ccatcgcgcg cttcatgaca gagcgactgg gtgcgcggcg cgccatactg gcggtggtgc
     2341 tcgcgggtgc attggtaacg tatggcggcg tcagcctgtt cgtcgccttc tttgtcctgg
     2401 cgccgatggc gcaggcgctt tttcaggccg ctgacattcc gcggcggttg atgccggcgg
     2461 caattgctct tggcacctcg acgttcacga tgacggcgtt ccccggaacg cccgccattc
     2521 agaatgcgat tccgatgccg tttttcggta ccacgccgtt tgccgcgcct ggcctcggca
     2581 tcatcggctc ggcaatcatg ctgggatttg gattgtggtg gctcgcccgt tccgaagccg
     2641 ccgctcggcg ggcgggtgag ggccatggtg gtgatactga catctccccg cgctcggtcg
     2701 ccgatgatca gatcatccgg gagcgggcta cgaccgcacg cgaattcgat cccgcggaga
     2761 tcggccacgg ccgtcatggc gaaaccccgg tgccgattgg cattgccgtg ctgccgctgg
     2821 ttgttgtcgt aagtgtcaat ctcctgatgt cgctcgtcat tctgccgcgc ctggactttt
     2881 cgttcctggc ggaagcgcat tggggcgcca cctcgctgtc agccgttgcg ggtgtttggt
     2941 cggtggcggt ggcactcgcg accgcgatcc ttgtgcttgt ccttttcaac ggtgcgcggt
     3001 tgccggcctt gcgcgagagc atggatgccg gcgccaacgc ctccgtcttg ccggcgttca
     3061 gtgtcgccag cctcgtgggc ttcggtgccg tcgtcgcgat gctgcccgcg tttgcggcgg
     3121 tgcgcgattg ggtgctttca atcggaggcg gaccgttggt ctcgcttgcg gtagcaacca
     3181 acatactcgc cgctctgacc ggttcggcct cgggtggcat gacgatcgcg ctcgatgcgc
     3241 tcggcgacac ctatatgcgc atcgcggcgg agctaggtat tgaccccgcg ttgatgcacc
     3301 gggtggccgc aattggctcc ggtacactcg acagcttgcc gcacaatggt gctgtcgtga
     3361 cgctgctcgc cgtttgtggc tcgacgcatc gcgaaagtta tctcgatatc gtcatggttg
     3421 ggatcgtcag cgctctcatc gccctcgtgg cggttattgc cttgggctcg atgtttggct
     3481 ccttct
//
LOCUS       DASUMG010000509         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_470478, whole genome shotgun sequence.
ACCESSION   DASUMG010000509 DASUMG010000000
VERSION     DASUMG010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470478"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..841)
                     /locus_tag="VFX46_08440"
     CDS             complement(5..841)
                     /locus_tag="VFX46_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX5600650.1"
                     /translation="MMRPDQNRRPLHRVITSLQNPTVKAVRALEMRKVRRETGLFVAE
                     GASVLVTARDQGWRPNTLLYPPEAAAGEVHRQLVEWARRSGAEILEVSEAVLAKVASK
                     ENPQSMLAVFQQAWHKLPEPAEIGPDTVWVALEEIRDPGNLGTIIRTADAVGAGGLIL
                     VGASCDPYSREAVRASMGSIFAVPLVRTDRGGFLAWRHSWPGEVVGTHLAATEDFRAV
                     SYRGPTLLVMGSEGPGLSEALTKACDRLVKIPMAGKLDSLNLAVATALALYQIRGPYL
                     KL"
     gene            complement(838..1779)
                     /locus_tag="VFX46_08445"
     CDS             complement(838..1779)
                     /locus_tag="VFX46_08445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX5600651.1"
                     /translation="MNRGSTSRTLRSANSLASARPATLSLVTTHGFPDYALLDSGGGR
                     KLERFGTLTVDRPEPQAMWQPRLPAKWSTAHAVFSGGEDEEKGRWRIDKPVPESWPVK
                     VAGVTMLCRLSAFRHLGLFPEQLPHWEWMLECLRRVTGGRPRVLNLFAYTGAASLIAA
                     KAGAEVVHVDASKKAIAWARENQAAARLSDAPIRWILDDARKFAAREVRRGRTYHMIL
                     VDPPKFGRGPANEVWELFEHLPGLMQDCARLLDPVCAHLCLTAYAIRASALSIDGLVR
                     EVLAGRGGQIESGELAIIEQSGGRALGTSLFTRWSSP"
     gene            complement(1929..>2086)
                     /locus_tag="VFX46_08450"
     CDS             complement(1929..>2086)
                     /locus_tag="VFX46_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX5600652.1"
                     /translation="YGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPMIKITNIVALLL
                     LAVLAQF"
BASE COUNT          366 a          696 c          673 g          351 t
ORIGIN      
        1 acgctcaaag cttcaggtag ggcccgcgga tctgatacag cgcaagcgct gtcgcgacgg
       61 ccaggttgag gctgtccaac ttgccagcca ttgggatctt gacgagccgg tcgcaggctt
      121 tcgtcagagc ctctgaaagg ccggggcctt cgctgcccat gaccaagagc gttggacccc
      181 gataggagac agcgcggaaa tcctccgtgg ccgccaaatg cgttcccacc acttcaccag
      241 gccatgagtg acgccacgcg aggaagcctc cgcgatcggt ccgcaccagc ggcacggcga
      301 agatggagcc catgctcgcg cgtaccgcct cgcgcgagta tgggtcgcag gaagccccga
      361 ccaggatgag tcctccggca cccaccgcat cagcggttcg aataatggtg cccaggttgc
      421 caggatcgcg gatttcttcc agagcgaccc agaccgtgtc gggcccgatt tcggcgggtt
      481 cgggcagctt gtgccaagcc tgctgaaaca ccgcgagcat gctctgcggg ttctccttcg
      541 aggcgacctt ggcaagcacc gcctcgctta cctccagaat ttccgcaccg gagcgccgcg
      601 cccattccac gagctgccga tgaacttcgc ccgccgctgc ctcgggcggg tagagcagcg
      661 tgttcggccg ccagccctgg tcacgcgccg tcacgaggac ggacgctccc tcggccacga
      721 acaacccggt ctcgcggcgc accttccgca tctcgagcgc gcgcacggct ttgacggtcg
      781 ggttttgcag gctcgtgatg acccgatgca aagggcgccg gttctgatct gggcgcatca
      841 tggggaggac caccgggtga aaagcgaggt gccgagcgcc cggccgcccg attgctcgat
      901 gatggccagc tctccgctct cgatctggcc gccgcgccct gcaagaacct cccgcacgag
      961 cccgtcgatg gagagcgccg aagcgcgaat ggcgtatgcg gtcaggcaga gatgcgcgca
     1021 aacaggatca agcagccgtg cacagtcctg cattagaccc ggcaggtgct cgaacagctc
     1081 ccacacctcg ttggcgggcc cccggccaaa ctttggcgga tcgaccagga tcatgtgata
     1141 ggtccggccc cgccgaacct cgcgcgctgc gaatttgcgc gcatcgtcga gaatccagcg
     1201 gatcggcgcg tcactcaggc gggcggctgc ttggttctcc ctggcccagg cgattgcctt
     1261 cttcgatgcg tcgacgtgca cgacctcggc gcctgccttc gccgcaatga gcgatgcggc
     1321 cccggtgtag gcgaagagat tgagcacccg cgggcgcccg cctgtgacgc ggcgcaggca
     1381 ctcgagcatc cattcccaat gcgggagctg ctctgggaag aggcctagat gccggaacgc
     1441 gctcagccgg cacagcatgg tgaccccagc gaccttcacc ggccaggact ccggcactgg
     1501 cttgtcgatc cgccaacggc ccttttcctc atcctcgccg ccggagaaca cggcatgagc
     1561 cgtggaccat ttggccggta atcgcggctg ccacatcgcc tgaggctctg gccgatcgac
     1621 ggtcagggtg ccgaaccgct cgagcttgcg tccgccaccg gagtcgagaa gggcgtaatc
     1681 gggaaatccg tgagtcgtga cgagcgaaag ggtggccggc cgcgcggagg ccaggctgtt
     1741 cgcggacctg agtgtccggg atgtcgatcc ccggttcatt cgcgaagctg cggtcgctgg
     1801 aggagcatgc gccctcatca cggaagctcg gcccgatgtc aatgtccgcg caagttgatg
     1861 tggcgaggcc agaaaagaga aggccccgcg aacggggcct tctggcatcg tgtctcaacg
     1921 ctgcagcttc agaactgcgc cagcacggcg agcagcagca gcgccacgat attggtgatc
     1981 ttgatcatcg gattcaccgc gggtcccgct gtgtccttgt aggggtcgcc gacggtgtcg
     2041 ccggtcaccg cagccttgtg ggcttccgag cctttgccgc cataat
//
LOCUS       DASUMG010000510         2655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_953185, whole genome shotgun sequence.
ACCESSION   DASUMG010000510 DASUMG010000000
VERSION     DASUMG010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953185"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFX46_08455"
     CDS             <1..745
                     /locus_tag="VFX46_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX5600653.1"
                     /translation="EVLIGYDGISIAQSRKAQPMDLTKTQIYLALAAEIPVDGKFAPN
                     PHKKWSDIDSSLPDTPILAYGPPPTSGTRDAFVELAMQEGCQALDFVKEQKAKLDKAA
                     YEELVKIQCSRMRQDGPFIEAGENDNLIVQRLEADPRAVGIFGYSFLYENEDKLQAIK
                     VEGVEPSFETIASGEYGISRPLYFYIKNAHREVVPGLKEFIAEYVSEAAMGEDGYLSE
                     RGMVPLQADALEKTRKAALEAERMAVPTN"
     gene            869..2248
                     /gene="pstC"
                     /locus_tag="VFX46_08460"
     CDS             869..2248
                     /gene="pstC"
                     /locus_tag="VFX46_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX5600654.1"
                     /translation="MLSYAFAVLLVLTLIGFLIGHRRAIASVEGRTYQLHSRPAYHAS
                     FVAAWVGIPATLLLLVWLLVQGPVLDRLIMASLPEEVVAGKGVGELSLILSQIKSVAG
                     GTSFGQVEPAILEAGRRLEAWRRIGEMAMFTSAVVLALLALVIVQSRISPDFRARQAV
                     ERILDWLMIACAVVAILTTLGIVISLLLEAIRFFRLVPVTEFLFGLRWEPQIAIRPDQ
                     ASAGGAFGAIPVFVGTFLIAFIAMAVAVPIGLLSAFYLTEYATSRFRAIVKPVLEILA
                     GIPTVVYGFFAVLVVSPALRTFGEALGIPIAPNSALVAGGVMGIMIIPFISSLSDDAL
                     TAVPSSMRDGSLAMGATKNETILRVLLPAALPGIMGGILLAVSRAIGETMIVVMAAGI
                     IATLTLNPFQPVTTVTVQIVTLLIGDTEFDNPKTLAAFALGLTLFLSTLVLNVIAIRI
                     VRRYREQYE"
     gene            2241..>2655
                     /locus_tag="VFX46_08465"
     CDS             2241..>2655
                     /locus_tag="VFX46_08465"
                     /inference="COORDINATES: protein motif:HMM:NF023240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3333 domain-containing protein"
                     /protein_id="HEX5600655.1"
                     /translation="MSEVRASEANSAAPPAAGHWHSASHHRRLKRRYAADRRLRMLGL
                     GAIGLAIGLLGILIVSLVYTGYVAFTQTRVELEFRIDPALVKPENVRSGNFRSVVRQA
                     IFEQFPEVTNRRQQTQLARLLSPGAEWIVRDYVVAH"
BASE COUNT          459 a          825 c          831 g          540 t
ORIGIN      
        1 agaggtgctg attgggtacg acggaatatc catcgcgcag tcccgcaagg ctcagccgat
       61 ggatctgacc aaaactcaga tctatctggc ccttgcagcc gagatccctg tggacggcaa
      121 gttcgctcca aacccgcaca agaagtggag cgacatcgac tcgtcgctgc ccgatacccc
      181 cattctcgcc tatggtccgc ccccgacatc cggcacgcgc gatgcgttcg tcgagctcgc
      241 catgcaggag ggctgccaag ctctcgactt cgtgaaagag cagaaggcaa agctcgacaa
      301 ggcggcttac gaggagttgg tgaaaatcca gtgcagccgc atgcggcagg acggtccatt
      361 cattgaggcc ggcgagaacg ataaccttat cgtgcagcgt ctcgaggctg atccgagggc
      421 cgtcggcatc ttcggctact cgttcctcta cgagaacgag gacaagctcc aggccatcaa
      481 ggtggaaggg gttgaaccgt cattcgagac gatcgcctcg ggggagtacg ggatctcgcg
      541 tccactatac ttctacatca agaacgctca tcgcgaggtg gttcccggac tgaaagagtt
      601 catagccgag tacgtgagcg aggccgcgat gggagaagac ggctacctct cggagcgcgg
      661 gatggtgccg ctgcaggcgg atgccctgga aaagacccgc aaggcggcgc tggaagcaga
      721 aagaatggca gttccgacca actgatgccc gttgaaaacg ccggctgcgc cattgacgtg
      781 gcgcagtcgc acgcgcttgt ggtgcacccg cagaacggac agtcacaccg gctcgcgcaa
      841 ttccgcaggc tcgacacatg aactcgacat gctgagctac gccttcgcag tcctgctcgt
      901 gctgacgctg attggatttc tgatcggcca cagacgggcc atcgcgtctg tcgagggacg
      961 cacctatcag ctgcattcgc ggccggccta tcacgccagc ttcgtcgcgg cgtgggtcgg
     1021 tatcccggcc accctgctgc tgttggtttg gctgctggtc cagggtccgg ttctcgatcg
     1081 gctcatcatg gcgtcgttgc cggaggaggt ggttgccggc aagggtgttg gcgagctcag
     1141 ccttattctg agccagatca agtccgtcgc cggaggcacg tcgttcggcc aagtggagcc
     1201 ggccattctc gaggctggaa ggcggctcga ggcgtggcgt cggatcggag aaatggccat
     1261 gttcacgagc gccgtggtgc tggccctgct ggcgctcgtc atcgtccagt cgcgcatctc
     1321 ccctgatttc cgcgctcgcc aggccgtcga gcgcattctc gattggctta tgatcgcctg
     1381 tgccgtggtc gcgatcctaa cgacgctcgg catcgtgatc tcgctcttgc tggaggccat
     1441 caggttcttt cggctcgtgc cggtcacgga gtttctgttc ggtttgcgct gggagccgca
     1501 aattgccatc cggccagatc aggcgtcggc aggcggagcc ttcggcgcca ttcccgtgtt
     1561 cgtcggcacg ttccttatcg ccttcatcgc catggcggtg gcggttccga tcgggctgct
     1621 gtcggcgttc tacctcaccg agtatgcgac atcgcgcttc cgggcgatcg tcaaaccggt
     1681 gctggaaatc ctcgccggca tcccaaccgt ggtgtacggc tttttcgctg tgctcgtcgt
     1741 ttcgccggct ttgcgcacgt tcggtgaagc gctcgggatt ccgatcgcgc ccaatagtgc
     1801 cctggttgcc ggcggcgtga tgggcatcat gattatcccg ttcatttcgt cgctgtcgga
     1861 cgacgcgctc acggccgtgc ccagctccat gcgcgacggc tctctggcga tgggcgcgac
     1921 gaagaacgag actatcctcc gcgtccttct gccggcggcg ctgcccggca tcatgggcgg
     1981 catcctgctg gcggtcagcc gtgccatcgg agaaaccatg atcgtcgtca tggcggccgg
     2041 catcatcgcc acgctgacac tgaacccgtt ccagcccgtc acgactgtca cagtccagat
     2101 cgtcaccctg ctgatcggcg atacggagtt cgacaacccc aagacgctgg ccgcgttcgc
     2161 cctgggcctg accctcttct tgtcaaccct ggtgctgaac gtgatcgcca tacgcatcgt
     2221 gaggcggtat cgagagcaat atgagtgagg tgagggcatc agaggcgaat tcggctgcgc
     2281 ccccggcggc gggccactgg cattcggcct ctcatcaccg tcggctgaag cgccgctatg
     2341 cagccgatcg gcggcttcgg atgctcgggc tcggagccat cgggctcgcc attggcctgc
     2401 ttggcattct gatcgtttcg cttgtctaca cgggctacgt agcattcacg caaacacgtg
     2461 ttgagctcga gttccgtatc gaccccgcgc tggtgaaacc ggagaacgtg cggagcggta
     2521 acttccgcag cgtcgtccgg caggcgatct tcgagcagtt tccggaggtc accaaccggc
     2581 gccagcaaac ccagcttgca aggctgctga gccccggcgc cgaatggatt gtgcgtgatt
     2641 atgtggtcgc ccatc
//
LOCUS       DASUMG010000511         2263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_150897, whole genome shotgun sequence.
ACCESSION   DASUMG010000511 DASUMG010000000
VERSION     DASUMG010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150897"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2263)
                     /locus_tag="VFX46_08470"
     CDS             complement(<1..>2263)
                     /locus_tag="VFX46_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007600686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX5600656.1"
                     /translation="SLLPTLTKEQIGGTPQVVTLGLVLFTLGIAAGSVAAAKASHLRP
                     NLALVPIGAVLMGLFSLDLAWVTSGLVPGAAEITPGELLATASGLRFAFDLFALAAAG
                     GLFIVPSFAAVQAWAPPQRRARVVAAVNVLNAAFMTAATAVVSALQYGGATTPLLLAG
                     LGLSNLLVVGLVLRAWGRQGVQDIASLIFRLAYRLEINGLEHIPPAGERVVVAPNHVS
                     LMDGPLMHAILPSHAAFAVDTTISQAWWVKPFLKLINAHTMDPTRPMAARTLVNLVKQ
                     GETLLIFPEGRITVTGGLMKVYDGAAMIADRADAWVLPIRIEGLERSKPWSYLRPSQI
                     RKAWFPKVRVTILPPRKLLVPADLKGKARRRAASAALQNIMIDTAVASARIDRTLFEA
                     LVEAKATRDTGKPIVSDALGTKLSYRKLILAAQVLGRKLEPMTPAGAAVGVMLPNSAG
                     VAVTFFALQSIGRVPAMINHTAGPQNVLSACRAANVEVVLTSRVFVEKARLGDLVERL
                     SLGVKVVYLEDIRAKITLADKIAGLLLGSRPQVPRDCNDPAVILFTSGSEGAPKAVVL
                     SHRNILANVAQSLTRIVANGDDTVFNALPVFHSFGLTVGLIMPLMAGAPVFLYPSPLH
                     YRIVPELVYQTNATILFGTDTFLNGYARVAHPYDLHSVRLVLAGAEAVKPSTRDLYMA
                     KFGVRILEGYGVTEAAPVLASNTPMANKPGTVGRLSPLMEVRLDPVDGIEDGGRLFVR
                     GPNIMLGYYRVENP"
BASE COUNT          477 a          679 c          709 g          398 t
ORIGIN      
        1 ccggattttc gacgcggtag tacccgagca taatattcgg accacggacg aataggcggc
       61 cgccatcctc gatcccgtct actggatcga gccgcacctc catgagcggt gacaggcgcc
      121 cgaccgtgcc gggtttgttg gccatcggcg tattgctcgc gagtacgggt gcggcttctg
      181 tgacgccgta cccttccaag atacgaacgc cgaattttgc catgtaaagg tcgcgcgtcg
      241 acggcttcac cgcctcggcc ccggcaagaa caagcctgac ggagtgcaga tcgtaggggt
      301 gcgcgacgcg cgcatagccg ttgaggaatg tatccgtgcc gaaaaggatt gtcgcgttcg
      361 tctgataaac gagctctgga acaattcggt agtgcagcgg cgacgggtaa aggaagaccg
      421 gcgcaccggc catcaacggc atgataagcc caaccgtaag gccgaaggag tggaacacgg
      481 gcagggcgtt gaaaacggta tcgtcgccat tcgctacgat gcgcgtcagc gactgtgcga
      541 cgttggcgag aatattgcgg tgggagagga caacggcctt gggtgctcct tccgatcccg
      601 acgtgaacaa gattacggcg ggatcgttgc aatcgcgggg gacctgcggc ctgctaccaa
      661 gcaggagacc tgcgatcttg tccgccaagg tgatcttcgc ccgaatatcc tccaagtaga
      721 caaccttcac gccaagggac aggcgttcga ccaaatcccc aagtcgcgcc ttttccacga
      781 aaacccgtga agtcagcacc acctcgacat tagcggcccg gcatgcagaa agaacgttct
      841 ggggtccggc ggtgtgattg atcatcgccg gaacacgacc aatgctctgc agtgcgaaga
      901 atgtaaccgc gacgcccgcc gagttgggca gcatgacgcc gaccgcagca ccagccggtg
      961 tcatcggttc gagcttacgg ccgagcacct gagctgcaag aatgagtttc cgatacgaaa
     1021 gcttcgtgcc gagggcatcc gatacgatcg gcttgccggt gtcacgcgtg gcctttgcct
     1081 cgacaagtgc ttcgaacagt gtacggtcga tccttgccga ggcgacagca gtgtcgatca
     1141 tgatgttctg caacgcagca cttgctgccc ggcgacgagc cttgcccttg aggtccgccg
     1201 gcaccagaag cttgcgcggt ggaaggatgg tcacgcggac cttcggaaac caggcttttc
     1261 tgatttgcga ggggcgcaaa tagctccagg gcttgctacg ctcgagacct tcgatccgga
     1321 tcggaaggac ccacgcgtca gctcggtcgg cgatcatcgc ggcgccgtcg tagaccttca
     1381 tgagaccgcc ggtgaccgtt atccgccctt cggggaagat cagcagggtt tcgccctgtt
     1441 tcacgagatt gaccaatgtc cgcgccgcca tcggccgagt cgggtccatc gtgtgggcat
     1501 taatcagctt cagaaatggc ttcacccacc aggcttgcga aatggtcgta tcgaccgcga
     1561 atgcagcgtg acttgggagg atcgcatgca tcagcggccc atccatgagg ctcacgtgat
     1621 tgggcgctac gacgacgcgt tcacctgctg gcggaatatg ctcgagccca ttgatctcga
     1681 ggcggtaagc gaggcggaaa atgagggatg cgatgtcctg aacgccttgc ctgccccagg
     1741 cccgcagaac cagtcccacc accaacagat tggataaacc caaccccgcg agcagcaacg
     1801 gagtggtcgc cccgccatac tggagcgccg atacgacagc ggtggctgcc gtcatgaacg
     1861 cggcgttgag cacgttcacg gcagcgacga cgcgggccct cctttggggc ggtgcccaag
     1921 cctgcacggc cgcgaatgac ggcacgatga agaggccacc agcggccgcg agggcaaaga
     1981 gatcgaaggc gaaccgcaga ccggacgctg tagcgaggag ttcacccggc gtgatctcgg
     2041 ccgcacctgg cactaatccc gaggtgaccc aggcaaggtc caggctgaac agccccatca
     2101 ggactgcacc gatcgggacc aaagcgaggt ttgggcgcag gtggcttgcc ttggctgcgg
     2161 cgactgaccc tgcggcgatc ccgagtgtga agaggaccag ccccaaggtg acgacctgcg
     2221 gcgttccccc gatttgctcc ttagtaagcg tcggcaatag gga
//
LOCUS       DASUMG010000512         4244 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_150960, whole genome shotgun sequence.
ACCESSION   DASUMG010000512 DASUMG010000000
VERSION     DASUMG010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150960"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /gene="rpsU"
                     /locus_tag="VFX46_08475"
     CDS             complement(<1..157)
                     /gene="rpsU"
                     /locus_tag="VFX46_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX5600657.1"
                     /translation="MQVLVRDNNIDQALKALKKKMQREGIFREMKLRNYYEKPSERRA
                     REKAEAIR"
     gene            complement(327..1436)
                     /locus_tag="VFX46_08480"
     CDS             complement(327..1436)
                     /locus_tag="VFX46_08480"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX5600658.1"
                     /translation="MSEGAVGGQPLPPGSTIGILGGGQLGRMLAVAAAKLGLRTHVYC
                     DEPRCPAFDVSALQTVGSYTDLDKLRAFAETVDVVTYEFENVPVDAARHLVEMVPVRP
                     GPRALEVAQDRLVEKEFISGLGVPVAPFARVNDASDLLAALEQFDAPVILKTRRFGYD
                     GKGQVGVAKGDDPAKALADIGNVPAILEKRVAFSCEVSVLVVRGMAGETAFYDIPQNI
                     HSGGILRRSVVPSPLGAAEVSRARDLARRIAEALEYVGVLAVEMFYMGQGAEPALLVN
                     EIAPRVHNSGHWTLDACSVSQFENHIRAIAGWPLGPTDRHSDAEMVNLIGADVLAWAE
                     LAAEPGALLHLYGKRQPREGRKMGHVTRLRPRSRL"
     gene            complement(1429..1932)
                     /gene="purE"
                     /locus_tag="VFX46_08485"
     CDS             complement(1429..1932)
                     /gene="purE"
                     /locus_tag="VFX46_08485"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX5600659.1"
                     /translation="MTASSAEAPKVGIIMGSQSDWPTMRRAAEALERLGVPFEAKIVS
                     AHRTPERLYSYAKSAKARGLKVIIAGAGGAAHLPGMVASLTTLPVLGVPIESRALKGQ
                     DSLYSIVQMPAGVPVGTLAIGAAGAENAGLLAAQILALEDPDLAARVEEHRARQTAAV
                     AEAPENE"
     gene            complement(2042..2248)
                     /locus_tag="VFX46_08490"
     CDS             complement(2042..2248)
                     /locus_tag="VFX46_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5600660.1"
                     /translation="MQWQDERDIRELLMKLRAEHRALDAEIAALEAQAYVDQLHITRL
                     KRKKLQLKDRIAALESQLLPDIIA"
     gene            2480..2653
                     /locus_tag="VFX46_08495"
     CDS             2480..2653
                     /locus_tag="VFX46_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEX5600661.1"
                     /translation="MSGSPHLAELEEKHRLLEQMIEEELARPAVDSIKLNSLKLQKLR
                     VKDQIARLAKNKR"
     gene            complement(2860..3747)
                     /locus_tag="VFX46_08500"
     CDS             complement(2860..3747)
                     /locus_tag="VFX46_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX5600662.1"
                     /translation="MLHAVLLAIAADGLLALMDAIVKTMTVRYSTFQIAFLRFGFGFA
                     WASLLLVCFRPGLPSRETIVFNTTRSVLAVITATSFFYALSQLPLADAVALAFLSPMF
                     TALFGALFLGERIDARIVTALMAGFAGLVLIAGGRVGSGTYSYGAVYGAIACVISAIT
                     YALTLVLLRARAQRDSFLIIVWFQNVGPALMLAPAAALAWTAMNARDLAIFILVGFLG
                     VAGHLLLASAFSRAEAARLAPIHYITLAWGVVFGYLFFAELPGLTTLAGAGLIVLGTI
                     ATHRPKSEAPATEADRRPV"
     gene            complement(3831..>4244)
                     /locus_tag="VFX46_08505"
     CDS             complement(3831..>4244)
                     /locus_tag="VFX46_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX5600663.1"
                     /translation="AEGNVHSVRLITRKPPAGLAGAPLLVEKGISVEGLKEPLKVFSG
                     TAREAAKGFPANLNVSVALSLAGIGPDRTDVEVWADPTVTHNTHTIIVRSDSSQLTMT
                     IENIPSAENPRTGVITPLSVIAALRRLTSPLVVGT"
BASE COUNT          861 a         1359 c         1258 g          766 t
ORIGIN      
        1 gccgaatggc ctccgccttc tcgcgggccc ttctctcaga gggcttctcg taataattcc
       61 ggagcttcat ctcccggaaa atgccctcgc gctgcatctt cttcttcagc gccttgaggg
      121 cttggtcaat gttgttgtcg cgaacgagta cctgcacgcg atctccttat cctgcttcgt
      181 tgaacctggt ttctgaacac aacaaaccat ccccacgtgc ccacaagcac ggggtggtca
      241 ttcattcgtc gggtgactca tatagcaaac accctcgccg tcgtccacgg gaacacgcaa
      301 gcttgaacca tcgcgagccc gctcactcac agccgtgacc tcgggcgaag cctggtcaca
      361 tggcccattt tgcggccttc gcgcggttgc cgcttgccgt agaggtggag tagcgcgccc
      421 ggctcggcgg ccagctctgc ccacgccagg acatcggcac cgatcaggtt caccatctcg
      481 gcgtccgagt gccgatccgt cggaccaagc ggccaacccg cgatggcacg gatgtggttc
      541 tcgaactggc tgaccgagca agcatccagc gtccagtggc cgctattgtg cacccgcggg
      601 gcgatttcgt tgaccagcag agcgggctcg gccccctgcc ccatgtagaa catctctacg
      661 gcaagaacgc cgacgtactc gagggcctcc gcaatgcggc gagcgaggtc acgcgcccgg
      721 ctcacctcgg ccgctccaag aggcgatggc acaaccgacc gccgcaggat gccgcccgaa
      781 tggatgttct ggggaatgtc atagaaggcg gtctcgccgg ccatgccccg caccaccagg
      841 accgagactt cgcaggagaa cgctacccgc ttctccaaaa ttgcagggac atttccgatg
      901 tcggcaagcg cctttgcggg atcgtctccc ttggcgacgc cgacctgccc ttttccatcg
      961 tagccgaagc gccgcgtttt caggatcacg ggcgcatcga actgctccag agcagcgaga
     1021 agatcgctgg catcgttgac cctagcgaag ggcgcgaccg gcacgcccag gccggagatg
     1081 aactctttct caacgagccg gtcctgcgcg acctccagcg cccgcggtcc gggccgcacc
     1141 ggaaccatct caaccagatg acgggcggca tcgaccggca cgttctcgaa ctcataggtg
     1201 accacgtcca ccgtctcggc aaatgctctt agcttgtcga gatccgtgta gctgccgacc
     1261 gtctgcaagg cagacacgtc gaacgccgga caccttggct cgtcgcagta gacgtgcgtc
     1321 cgcaggccaa gcttggcggc ggctaccgcc agcatgcgcc caagctgccc gccaccgaga
     1381 atgccgatcg tgctccccgg cggcaacggc tgcccgccga cggccccctc actcattttc
     1441 cggtgcctcg gccactgccg ctgtctggcg ggcgcgatgc tcctctaccc gggccgccaa
     1501 gtcaggatcc tcgagcgcca ggatctgggc tgccagcaat ccagcattct cggcccccgc
     1561 agcgccgatc gccagggtgc caaccggaac accggcgggc atctgcacga tggagtagag
     1621 gctgtcctgc cccttgagcg cgcgactctc tatgggcacg ccgagcaccg gcaacgtcgt
     1681 gagggaggcg accattccgg gcaaatgcgc tgctccgccc gctccggcga tgatcacctt
     1741 aagaccccgc gccttcgccg acttcgcgta agagtacaag cgttccgggg tgcggtgcgc
     1801 agaaacgatc tttgcctcga atggcacccc gagccgctct agcgcctcgg cagcgcggcg
     1861 catggtcggc cagtcggact ggctgcccat gatgattccg actttcggag cctcggcgct
     1921 gctcgcggtc atcttcaaat cttcatgccg cacaacggca atattcgtgg aaagacgcgc
     1981 aattacctgc cccgcagctc cttggcaaga gatcgagcgc ggctttgcac cgcaagcgtc
     2041 actacgcgat aatatccggc aggagctggc tctcgagcgc ggctatacgg tccttgagct
     2101 gcagcttctt ccttttgagc cgggttatgt ggagctggtc gacgtaggcc tgcgcctcga
     2161 gagccgcaat ctctgcgtcc agcgcgcggt gctccgcgcg cagcttcatc aacagctcgc
     2221 gaatgtctct ctcgtcctgc cactgcatcg gcgagctctg atggctgcaa atcgcgctct
     2281 ccgttcagcg cactatctgc gcgaacggcg gcggtgacaa gagtgtaaag cgccctgtgg
     2341 agcaggcaca gctgccgatg ggatcgtagc tcagcctgcg cctgtgcttc cgtattgttg
     2401 atgctaatga tcgacttatt agacaattaa ccccatttct ggcaagtttt acctgtcgag
     2461 aactcgatcg gagggcaaga tgtcggggtc cccacatctg gctgaactcg aagagaagca
     2521 ccggttgctt gagcagatga tcgaggaaga actggcgaga ccggccgtgg attccatcaa
     2581 gctcaacagc ttgaagcttc agaagctccg ggtgaaggac caaatcgccc gcctggctaa
     2641 gaataagcgc tgaggagccg gtttccggaa aaacggaccg ggcctactgc cggagcggca
     2701 cagaggccga gcatttcggc caaggacttt ttcaaggcgg aggaccgaag cgtcttccaa
     2761 ggaggtgtgt tacgcggcta tcgctccgca gccttgcgca ccgcaacagc aagaaggccc
     2821 aggcgctgcc cccggaacaa ttcggtctga gtttctaatc tatacgggac gtctatctgc
     2881 ctcggtggct ggcgcctctg atttcggacg gtgggtcgcg atcgtcccaa gcacgatcag
     2941 gcctgcaccc gccagcgtcg tgagacctgg aagctcggcg aagaacaggt agccgaagac
     3001 cacaccccaa gcgagagtaa tgtaatggat cggagccaac cgggccgctt ctgcgcgcga
     3061 aaacgccgaa gccagcagaa gatgtccggc cactccaagg aaccccacga gaatgaaaat
     3121 cgcgagatcg cgagcgttca tggccgtcca ggccaatgcc gcagcgggcg caagcatgag
     3181 tgcaggtccc acattttgaa accatacgat gatgagaaac gaatcccgtt gagcacgcgc
     3241 cctaagcagc accagcgtca aagcgtaagt aattgctgag atgacacacg cgatggctcc
     3301 gtagacggcg ccataactgt aagttcccga gccgacccgg ccgccggcta tcagcacgag
     3361 accggcgaaa ccggccatca gcgccgtcac aattcgcgca tcgatccgct cgccgaggaa
     3421 aagtgctccg aacagagccg tgaacatcgg cgacaggaac gccagcgcga ccgcgtctgc
     3481 gagtggaagc tggctcaagg catagaagaa cgaggttgcc gtaatgacgg caagcacgga
     3541 gcgggtggtg ttgaagacga tcgtctcccg gcttggcaaa ccgggtcgga aacagaccag
     3601 cagcagactc gcccaggcaa atccgaaccc aaatctcagg aatgcgattt gaaaagtcga
     3661 ataacggacg gtcatcgttt tgacgatggc gtccatgagc gccaacaggc catccgcggc
     3721 aatggccagg agcacggcgt gcagcattcc tccctcgcgt gtgcgggcga ctgacaatgc
     3781 agcgcttctc tattgatggt ctatgcggcg agctaacgct gaaattgatg tcacgtgccg
     3841 acgaccaacg gcgatgtgag cctgcggagc gccgcgatga cgctcaacgg ggtaatcacc
     3901 ccggttcgcg gattctcagc cgacgggatg ttctcgatcg tcatcgtgag ctgggacgag
     3961 tccgacctca cgatgatcgt gtgggtgttg tgtgtgaccg ttgggtctgc ccaaacctcg
     4021 acgtcggttc ggtcagggcc gataccggca agggaaagcg ccacggacac attgaggttg
     4081 gcaggaaagc ctttcgccgc ttcacgggct gttcccgaga acactttcag cggttccttc
     4141 agtccctcca ccgaaatccc tttttcgacg agcaaaggcg caccagccag gccggctggc
     4201 ggtttgcgtg tgatgagacg cacggaatgg acattcccct cggc
//
LOCUS       DASUMG010000513         2845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_712202, whole genome shotgun sequence.
ACCESSION   DASUMG010000513 DASUMG010000000
VERSION     DASUMG010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712202"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1642)
                     /locus_tag="VFX46_08510"
     CDS             complement(161..1642)
                     /locus_tag="VFX46_08510"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HEX5600664.1"
                     /translation="MSEQKRPVLTTSAGAPVADNQNSLTAGARGPLLLQDYQLIEKLA
                     HQNRERIPERPVHAKGWGAYGTLTITGDISKYTKAKCLQPGAKTELIARFSTVAGELG
                     AADAERDVRGFALKFYTEEGNWDLVGNNTPVFFVRDPYKFPDFIRTQKRHPKTNLRSP
                     TAMWDFWSLSPESLHQVTILFSDRGLPQSARHMNGYGSHTFSFISPDNKRYWVKFHFK
                     TLQGHKYWTNAEAREVIGRTRESTQEDLFYSIERGDFPRWKMQVQIMPEEDADKHWYN
                     PFDLTKVWPHADYPPIDVGILELNRNPENYFAEIEQAAFSPSNIVPGIGFSPDKMLQA
                     RVFSYADAHRYRLGTHYEMLPVNRPRCPVHHYHKDGPMRFFEPLTGNVDAYYEPNSFG
                     GPVQDDRFREPPLKISGDADRYNHRDGNDDYRQPRALFELFDDGQKQRLFMNIAESMV
                     GVPQFIIDRQLGHFEKVHPDYAAGVRKALAEQAAQTGHQQAAE"
     gene            1739..2677
                     /locus_tag="VFX46_08515"
     CDS             1739..2677
                     /locus_tag="VFX46_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HEX5600665.1"
                     /translation="MQPVRLTRLTLRQMVYFEAVARHRHFGRAAEACAVSQPALSVQI
                     QELERELGVSLVERNRGGVTLTPQGEYVLQRVRRILGEVQDLMDCATQIEGFGPIRLG
                     VIPTIAPYLMPAILEAFGLENASMQLQVRETQTHRLIEELEQGNIDLMLAALPISSPV
                     IEVQPLFRDRFFLVAPADSKIGPRIDSVAAIGRYRLLLLEEGHCLREQALSVCSMADK
                     DMLASLGATSLATLIELVAAGMGVTLLPELCLPSLHGDQRVRVVPFRGPAPFRTIGLA
                     WRKTSGRKAVFRRLGELIAEAGRPRKGAQSKRNPVR"
BASE COUNT          513 a          855 c          888 g          589 t
ORIGIN      
        1 aaaggatgca cattacatca gtagtatgcg catcccgatc acctccctgc tatgaccagg
       61 aagcctcaca tccccggttt tctgccaggc acgcaccaaa caaaaaagag ccggacgctc
      121 cgtccggctc ttctgctttg gtctttggac agtcgggctg ctactcggcg gcctgctgat
      181 ggccagtttg agcagcctgt tcggccagcg ccttcctgac acccgccgca tagtccgggt
      241 gcaccttttc gaaatgcccg agctgccgat cgatgatgaa ctgaggcaca ccgaccatag
      301 attcggcaat gttcatgaag agccgctgtt tctgtccgtc gtcgaacaac tcgaacagcg
      361 cccgcggttg gcggtagtcg tcgttgccat cgcgatgatt gtagcggtcc gcgtcgcccg
      421 agatcttgag cggcggctcg cggaagcggt cgtcctgcac aggaccgccg aatgagttcg
      481 gctcgtaata cgcatcgacg ttgccagtca gcggctcgaa gaagcgcatc ggtccatcct
      541 tgtgatagtg gtggaccggg cagcgcggcc ggttgaccgg cagcatctcg taatgcgtgc
      601 cgagccggta acggtgcgca tcggcatagg agaacacgcg cgcctgcagc atcttgtccg
      661 gcgagaagcc aatgcccggc acgatgttcg acggcgagaa ggccgcctgc tcgatttcgg
      721 cgaagtagtt ctccggattg cggttgagct ccaggatgcc gacatcgatc ggcgggtaat
      781 ccgcgtgcgg ccagactttc gtcaggtcga acggattgta ccaatgcttg tccgcatcct
      841 cctcgggcat gatctggacc tgcatcttcc acctcgggaa gtcgccccgc tcgatggagt
      901 agaacagatc ctcttgcgtc gactcacgcg tgcggccgat gacctcccgg gcctcagcgt
      961 tggtccagta cttgtggccc tgcagggtct tgaagtggaa cttgacccag tagcgcttgt
     1021 tgtccgggct gatgaacgag aaggtgtgcg agccgtagcc gttcatgtgg cgcgcgctct
     1081 gcggcaggcc gcggtccgag aacaggatcg tcacctgatg aaggctctcc ggcgatagcg
     1141 accagaaatc ccacattgcc gtcggcgaac gcaggttcgt ttttgggtgc cgcttctggg
     1201 tgcggatgaa gtccgggaac ttgtaaggat cacggacgaa gaagaccggc gtgttgttgc
     1261 cgacgaggtc ccagttgccc tcttccgtat agaacttgag tgcgaagccg cgcacgtcgc
     1321 gctctgcgtc ggcggcgccg agctcgcccg caacagtcga gaaccgggcg atcaattcgg
     1381 tctttgcgcc tggctgcagg cacttcgcct tggtgtactt cgagatgtcg ccggtgatgg
     1441 tcagcgtgcc gtaggcgccc cagcctttcg catgcacggg acgctccgga atgcgctcgc
     1501 ggttctgatg ggccagcttc tcgatgagct gatagtcctg aagcagcagc ggcccgcgcg
     1561 caccagccgt cagcgagttc tggttgtcgg cgacaggtgc gccggctgat gtggtcagaa
     1621 cgggtcgttt ctgctcgctc atgccattcc tcctatgtca gtggtcagct ggaaactggc
     1681 ccggaggtgc gataaacgca aatacctctt tccgatcatt caataggagt ttcttatcgt
     1741 gcagcctgta cgcctgacgc ggctgacctt gcggcagatg gtctacttcg aggccgtggc
     1801 ccgtcaccgg catttcggcc gcgcggcgga ggcctgcgcg gtctcgcaac cggcgctgtc
     1861 agtgcaaatc caagagctgg aacgggagct gggcgtgtcg ctcgtcgagc ggaaccgcgg
     1921 tggtgttact ctcaccccgc aaggcgagta tgtgcttcag cgagtccgcc ggatcctcgg
     1981 cgaggtgcag gatctcatgg actgtgcaac tcagatcgag gggttcggtc cgatccgcct
     2041 tggcgtcatc ccgacaatcg cgccttatct gatgccggcc attctggagg ctttcgggct
     2101 ggaaaatgcc tccatgcaac ttcaggtgcg cgagacgcag acgcatcggc tgattgaaga
     2161 gctggagcag ggcaacatcg acctcatgct ggccgccttg cctatctcga gccctgtcat
     2221 cgaggttcaa ccgctatttc gcgatcgctt cttcttggtg gccccggcag attcgaagat
     2281 aggtccacgg atcgatagcg ttgccgccat cggccgttac cggcttctgc tgctagaaga
     2341 gggacattgt ttgcgagagc aggcgctcag cgtctgtagc atggctgaca aggacatgct
     2401 cgcgagcctc ggcgccacca gtctcgcgac actgatcgag ctcgttgcag ccggcatggg
     2461 cgtgaccctc ttgccggagc tctgcttgcc atctcttcac ggggatcagc gcgttcgtgt
     2521 cgtgcccttc cgcggcccag cgcccttcag aacgatcggg ctggcatggc gaaagacgtc
     2581 tggccgaaag gcggtgttcc gacgactggg tgagctgatt gcggaagcgg gaaggccgcg
     2641 gaaaggggca cagtccaagc gtaatcccgt tcgctaaacc gccagccggc cgcaacggcc
     2701 atccaccatc aaatatccaa gtccttggcg aatgccgcgc gctcctggat gaagcggaaa
     2761 cgggcttcgg gcttggagcc cataaggttg ttcaccgctt ctgcaattgc actacggtct
     2821 ccatcgccga gttcgacgcg gagaa
//
LOCUS       DASUMG010000514         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_712215, whole genome shotgun sequence.
ACCESSION   DASUMG010000514 DASUMG010000000
VERSION     DASUMG010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_712215"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..571
                     /locus_tag="VFX46_08520"
     CDS             74..571
                     /locus_tag="VFX46_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEX5600666.1"
                     /translation="MTAALGRNRAPLHDGRQSPAALHIARGVGRVLASLGLASISELP
                     LPNGRRADIVALSEKGDIWIVEIKSSIEDFRADQKWPEYWDYCDRLFFAVSPAFPQEL
                     LPHETGIIVADRYGGEIVRFVEESRLPAHRRRAMALRFGRVAAARLQSVADPGLALET
                     LLRGD"
     gene            complement(634..1197)
                     /locus_tag="VFX46_08525"
     CDS             complement(634..1197)
                     /locus_tag="VFX46_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HEX5600667.1"
                     /translation="MAEQFAFSKEELPEDTSLVIVDDDRAFLQRLGRAMESRGFEVRT
                     AASVAEGMELIRQKPPAFAVVDMRLEDGNGLDVIAELTRIRPNARGIILTGYGNIATA
                     VSAVKLGAVDYLAKPADADDVTDALLAPPNAKAPPPENPMSADRVRWEHIQRVYELCN
                     RNVSETARRLNMHRRTLQRILAKRAPK"
     gene            complement(1295..2665)
                     /locus_tag="VFX46_08530"
     CDS             complement(1295..2665)
                     /locus_tag="VFX46_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HEX5600668.1"
                     /translation="MTGWLGLQERGVEVEQVESRLRLHTIVRLRWFAVLGQLLAVSIV
                     RFVLEFEFPFSLCLLLIAATAWLNVFLSVRYPARFRLGATFATVMLAYDILQLVALLY
                     LTGGIENPFVVLIVGPVTVSAATLPPINTVFLGGLALISTAFLTVVHWPVPWYPDELL
                     TLPLIYKIGQLAAITATILFLALYVWRLSKEARQMSAALTATELVLAREQKLHALDGL
                     AAAAAHELGTPLATIVLVTSELQKEIEPNTPLAEDMALLRSQAERCREILQKLTRQPS
                     EQDPLHATLSVSQLLDEAAEPYRSGTKAIQITCQPFEGVSGPAAKEPVGERRPGLIYG
                     VGNIVENAVDFAHSKVEITAHWDERTLEVEVKDDGPGFRPELLETLGEPYVTTRPAGT
                     GGPGKRAAGLGLGFFIAKTLLERSGASLTLDNRPAPERGAIVRISWAREVFEAAPAGP
                     QRVEAA"
     gene            2809..>3029
                     /locus_tag="VFX46_08535"
     CDS             2809..>3029
                     /locus_tag="VFX46_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600669.1"
                     /translation="MLQAGARTDGAGAAPPVEVRDLRKVYGDVVAVDGVSFRLNQGSV
                     TALLGGNGAGKTTTIAMLMGLVIPTSGEVR"
BASE COUNT          578 a          970 c          937 g          544 t
ORIGIN      
        1 aagcaggaaa gtgagacacg gaccagcgcc tcgcgagaaa gttgcggaaa tgtcaccaac
       61 agatggccaa cgcatgacgg cggcgctcgg tcgaaaccga gcgccgcttc acgatggacg
      121 tcagtcaccc gccgcgctgc acatcgcgcg aggcgtggga cgcgttttgg ccagcctcgg
      181 cctcgccagc atctccgaac taccgctgcc aaacgggcgc cgtgccgaca tcgtggcgct
      241 gtccgaaaag ggcgatatct ggatcgtcga gatcaagtcc agcatcgagg acttccgcgc
      301 tgaccagaag tggcccgaat actgggacta ttgcgaccgc ctcttcttcg ccgttagccc
      361 ggcattcccg caagagctgc tgccacacga gacggggatc atcgtagccg atcgatacgg
      421 cggcgagatc gtacgtttcg tcgaagaatc ccgcttaccg gcacaccgac gccgcgcgat
      481 ggccttgcgt tttggacgcg tggccgctgc acgactccaa tcagtcgccg atcccggact
      541 ggcgctcgaa acgctactac gcggcgacta gcacagatgc tcctgctcct ggcggtgagg
      601 cccccggccg gtcagaaacc agcaaagaat cgcttacttg ggggcccgct tagccagaat
      661 gcgctgcagc gtccgacgat gcatgttcaa gcggcgcgcc gtttctgaca cgtttcggtt
      721 gcacagctcg tagacgcgct ggatgtgctc ccaacgcacc cggtctgccg acatcgggtt
      781 ctccggtggc ggagccttgg cgttaggcgg cgccagcaac gcatcggtga cgtcatccgc
      841 gtcagcgggt tttgcgaggt agtccacggc gcccagcttc accgctgaaa cagccgtcgc
      901 aatattgcca tagcccgtga ggatgatacc gcgcgcgttt ggacgaatgc gcgtcagctc
      961 agcgatgaca tcgaggccat tgccatcctc gagcctcatg tcgacgacgg cgaaggctgg
     1021 cggcttctgg cggatcagct ccatgccctc ggcgaccgag gccgccgtgc gcacctcgaa
     1081 cccccgcgat tccatggcgc ggccaagacg ctggaggaac gcccggtcat cgtccacgat
     1141 gacgagggag gtatcctctg gaagctcttc cttactgaat gcgaattgtt ctgccataac
     1201 caactcctgc atgccctaca tatagcggtc gaaatgccgc atccaagacc catgtttcta
     1261 cttttgtccc attgggttcg ggttgcggct gccactaggc tgcctcgacg cgttgggggc
     1321 cggcaggtgc tgcttcgaac acttcccgcg cccaactgat gcgaacaatg gcgccgcgct
     1381 ctggtgccgg gcggttgtcc agagtgaggc ttgcgccaga acgttcgagc agcgtttttg
     1441 cgatgaagaa ccccaggccc agccccgcgg ctcgcttgcc aggtccgcct gtcccagctg
     1501 gacgtgtcgt gacatagggc tcgccgagcg tttcgaggag ctcggggcgg aatcccggcc
     1561 cgtcgtcctt cacctcgacc tcaagcgtgc gttcgtccca gtgggccgtg atttcgacct
     1621 tactatgggc aaagtcgacc gcgttctcga cgatgttgcc gaccccgtag atgagaccgg
     1681 gcctccgttc cccgaccggc tccttcgctg cgggaccgct cacgccttcg aaaggctgac
     1741 atgtgatctg gatggccttc gtcccactgc ggtagggctc ggccgcttcg tcgaggagct
     1801 gggaaaccga tagcgtggca tgcaatggat cctgctcgct cggttgccgc gtcagcttct
     1861 gcagaatctc acggcaacgc tccgcctggc tgcgcagcag ggccatgtcc tcggcgaggg
     1921 gcgtgttcgg ctcgatctcc ttttgcagct cgctcgtaac caggacgatc gtcgctagcg
     1981 gggtgccgag ctcatgggcc gcggctgcag ccagtccatc cagcgcgtgc agcttttgtt
     2041 cacgggcgag gacgagttct gtggctgtca gggcagccga catttgccgc gcctccttcg
     2101 acaggcgcca gacatagagc gcgaggaaca gaatggtcgc cgtgatggcg gcaagctgcc
     2161 cgatcttgta gatcagcggc agtgtcagaa gctcgtcggg gtaccacggc accggccaat
     2221 ggacgacggt gaggaacgcc gtggagatga gcgccaagcc gcccagaaag accgtgttga
     2281 tcggcggcag ggtcgcggca gatacggtca ccggccccac gatcagcacc acgaatgggt
     2341 tctcgatgcc gcccgtgagg tagaggagcg ccacgagctg gagaatgtcg tacgcgagca
     2401 taacagtcgc gaaagtggca ccgagccgga accgagccgg gtagcgcaca ctcaggaaaa
     2461 cgttgagcca ggccgtcgca gctatcagca ggaggcaaag gctgaaagga aattcgaact
     2521 ccagtacaaa acgaacgatg ctcacggcca acagctggcc gagcacggca aaccacctga
     2581 ggcgcacgat ggtgtgcaac ctcagccggc tttccacctg ctcgacttcg acgccccgtt
     2641 cttggagccc caaccagcca gtcaccacga cagaaccttc gctttccaaa tcacgcttgc
     2701 gccaaggcag ctaaacgggt tgcgccgcgc gccccatttg accacatagg tcgagactca
     2761 acacccgatc atcaatggcg ttgctcgccc ggcgccggac atagcatcat gttgcaagct
     2821 ggagctagaa cagatggcgc cggtgctgcg cctcctgtag aggtgcgcga tctgcgcaag
     2881 gtctatggcg atgtcgtcgc ggtggacggc gtctcattcc gcttgaacca gggctcagtc
     2941 accgcgttgc tcggcggaaa cggcgccggc aagaccacga ccatcgcgat gctgatgggc
     3001 ctcgtcattc ccacctcagg cgaggtgcg
//
LOCUS       DASUMG010000515         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1114222, whole genome shotgun sequence.
ACCESSION   DASUMG010000515 DASUMG010000000
VERSION     DASUMG010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114222"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFX46_08540"
     CDS             complement(<1..191)
                     /locus_tag="VFX46_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007630424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX5600670.1"
                     /translation="MRSLQIAAAVLALLLPGAAPAELRVVVTSKPIHSLVSSVMAGVA
                     EPDLLVDGTASPHSYAMKPS"
     gene            523..768
                     /locus_tag="VFX46_08545"
     CDS             523..768
                     /locus_tag="VFX46_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX5600671.1"
                     /translation="MARVTLVGTLRQFTGGIAEIEIEAGTVRQLFARLAERFPDLAPH
                     LEQGLAVAIDGQIYQDALLEPIGRDAEVHILPQIAGG"
     gene            complement(822..2351)
                     /locus_tag="VFX46_08550"
     CDS             complement(822..2351)
                     /locus_tag="VFX46_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX5600672.1"
                     /translation="MYGRISTLAFVGIEARPVQVEVRISPGQHVFAIVGLPDKAVAES
                     RERVRNALHAIGLGLPWTHITVNLAPADLPKEGSHFDLPIALALMVAMEAISPDAIQG
                     FAAIGELALDGQIRATPGALPAAIGANAMNKGLICAAENGPEAAWAAADMDIIAAPHL
                     LSLVNHFKGLQILRRPEPNLARDDQPLPDLADIKGQETARRALEVAAAGGHNLLMIGP
                     PGSGKSMLAARLPSILPPLDPQEMLEVSMVHSLAGELMGGRLKVERPFRAPHHSASMA
                     ALVGGGTRPKPGEVSLAHLGVLFLDELPEFSPQVLDSLRQPLETGETVIARANHRITY
                     PSRIQLVAAMNPCRCGGGSPGQTCKRGPRCAADYQARISGPLLDRIDLQIEVPAVTAA
                     DLVLPAPAEGSAAVRARVIAARERQRQRFAKYGANGPRTNSEASGRILEEIARPDADG
                     IALLRQAAETLQLSARGFHRTLRVARTLADLDGAETVSRIHIAEALSYRGEVLRQRQA
                     A"
     gene            complement(2462..>2649)
                     /locus_tag="VFX46_08555"
     CDS             complement(2462..>2649)
                     /locus_tag="VFX46_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEX5600673.1"
                     /translation="LGPELKKRGLLFTGIDVIGPYLTEINVTSPTGIRQIEAFGGPNI
                     AALFWDAVEARTARASL"
BASE COUNT          541 a          808 c          816 g          484 t
ORIGIN      
        1 gaaggcttca tcgcatagga gtgcggtgaa gccgtaccgt cgacaagcag gtctggctcg
       61 gccaccccag ccataacgct cgaaacgagc gaatgaatgg gcttgctcgt aacgaccaca
      121 cggagctcag ccggagctgc accgggaagc agcagagcta gaaccgcggc agctatttgt
      181 aaggagcgga ttgcgatcac cttttcctcc gctttcgaac tctggatggc tcgaaacgct
      241 gatcgggacg gaaccacaga gatagaacgt tataacatat cattcgaggc gtgaagctga
      301 ttatcgcatc gatgagacag tgcacatcaa agctcgggcc gccacacgca agcgtgtcgt
      361 tgtgggaccg ctcggcagcc tccgattcga atgctaaagg gccaatgaac gcatcaagac
      421 agcaacgagc ttcaccggct cggagcttgt tctgctactg cgtacatcat ttttcgtggc
      481 tgcgtaactt cgggcagcgc tagacgggga aagtggacgg cgatggctcg cgttactcta
      541 gtaggaactc ttcgccagtt caccggaggg atcgctgaaa tcgaaatcga ggctggaacg
      601 gttcgccagc ttttcgctcg cctcgccgaa cgctttccag acctggcgcc gcatttggag
      661 caggggctcg ctgtcgccat cgacgggcag atctatcagg atgctctgct cgagccgatc
      721 ggtcgagatg ccgaggttca cattctgccg cagattgctg gtggctagcc ggtccgatta
      781 gcaggctcgt taagccacta tcgaacgaac cgaagcacac ttcatgcggc ctgccgctgt
      841 ctgagcacct caccgcggta actcagggcc tccgctatat gaatgcggga tacggtctcg
      901 gcgccgtcga gatcggcgag tgttcgggct acgcgcaaag tccggtggaa accgcgggca
      961 gatagctgca aagtctcagc tgcctgacgc aggagagcaa tgccgtcggc gtcgggccgg
     1021 gcgatctctt cgaggatccg cccagatgcc tcagaattgg tgcggggccc atttgctccg
     1081 tatttggcaa accgctgacg ctggcgctcg cgcgcagcta ttacacgagc ccgcaccgct
     1141 gcgctccctt cagcgggcgc tgggagaacc agatcggcgg ccgtgaccgc cggtacttcg
     1201 atctgcagat cgatacggtc gagaagaggg cctgagatac gggcctgata gtcggccgca
     1261 caacgtggcc ctcgcttgca cgtttggcct ggagagccac caccacaccg gcaaggattc
     1321 atagccgcga cgagctgaat ccgcgaagga taggtgatgc ggtgattggc acgggcgatc
     1381 actgtttcgc cggtctcgag cggctgccgc agggagtcga gcacctgcgg cgagaactcc
     1441 ggtaactcat cgagaaataa gaccccaagg tgcgcgagtg agacctcgcc tggcttgggt
     1501 cgcgtgcctc cgcccacaag cgcggccatg ctcgcagaat gatgcggtgc ccggaacggg
     1561 cgttcaactt tcaacctgcc gcccatcagc tcgccggcaa gtgaatgcac catgctgact
     1621 tccagcatct cctggggatc gagcggcggc aatatggagg gcaagcgtgc tgccagcatc
     1681 gatttaccgg accccggcgg tccgatcatc agcaaattat gtcccccggc tgccgccacc
     1741 tcgagtgcac ggcgggcggt ctcctgcccc ttgatatcgg ccaagtcggg caggggctga
     1801 tcgtctcgtg ccaagttcgg ctccggccgg cgcaagatct gcaggccctt gaaatggttc
     1861 acgagcgaga gcagatgcgg ggcggcgatg atgtccatgt cggccgctgc ccaagccgct
     1921 tcggggccat tttccgcggc acagatcagc cccttgttca tagcattggc gccgatggcc
     1981 gctggcaagg caccaggcgt cgccctgatc tgaccgtcga gagccagctc gccgatcgcc
     2041 gcgaaacctt ggatggcatc aggcgagatc gcctccatcg caaccatcag cgctagcgcg
     2101 atcggcaaat cgaaatgact gccttccttc ggcagatccg ccggggcgag attgaccgta
     2161 atgtgggtcc acggaaggcc cagaccgata gcatggagcg cattccgcac acgctcgcga
     2221 ctttccgcga cggccttgtc cggtaaaccg acgatggcaa agacgtgctg tccggggctg
     2281 atcctgactt ccacctgaac cgggcgcgcc tcgataccga caaacgccaa cgtggaaata
     2341 cgcccgtaca tgctgtcccc cgcgtctccc ccgacttctt gtcgggggag gttaaaggcg
     2401 gaggacgacg cccgtcaaga acataatgag aacagacgaa agtagccgtt ctgccctcgg
     2461 atcaaagcga agcacgcgcc gtccgggctt ctacagcgtc ccagaagagg gctgcgatgt
     2521 tcgggccgcc aaatgcttcg atctgacgaa tgccggtcgg tgaggtcaca ttgatctcgg
     2581 tgaggtaggg accgatcacg tcgatgccgg tgaagagcag cccgcgcttc ttcagctccg
     2641 gcccgagcc
//
LOCUS       DASUMG010000516         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_151353, whole genome shotgun sequence.
ACCESSION   DASUMG010000516 DASUMG010000000
VERSION     DASUMG010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151353"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..559)
                     /locus_tag="VFX46_08560"
     CDS             complement(89..559)
                     /locus_tag="VFX46_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEX5600674.1"
                     /translation="MAGLDDVYNSRIFELAAEIPRQRRLPDPDATATAHSKLCGSTVV
                     VDLKVDASGRVTDFGQTVKACLLGQAAASVMGREIIGSTPDELREVGRTMRRMLKENG
                     PPPTGRWADLAILEPVRDYKARHASTLLVFDAVERALDEIEARRSGANQAVAAQ"
     gene            739..1371
                     /gene="folE"
                     /locus_tag="VFX46_08565"
     CDS             739..1371
                     /gene="folE"
                     /locus_tag="VFX46_08565"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEX5600675.1"
                     /translation="MTNISAAPFKNKVTHARRPTKQEAEEAVRTLIAWAGDDPDREGL
                     KETPRRVVEAFQEYFRGYGEDPVAMLQQPTFEEIGGYDDLVMLRDIRVESHCEHHIAP
                     FIGVAHVAYLPNGKVAGLSKLARVVEAYAKRLQTQETLSAQIADAIQEALSPRGVAVM
                     IEAEHQCMTMRGVHQPGVAAVTTRFLGAFATDSALRDRFLSLANASRRGR"
     gene            1502..1927
                     /gene="hisI"
                     /locus_tag="VFX46_08570"
     CDS             1502..1927
                     /gene="hisI"
                     /locus_tag="VFX46_08570"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEX5600676.1"
                     /translation="MASPSPLFAPRGSTEEIERGDVFQPKFGPDGLIPAIITDADTGD
                     VLMFAWMNAEALALTIETGLAHFWTRSRQRIWKKGEESGNLLRVREIRTDCDQDVLWL
                     KAHVEGNGVACHTGARSCFYRALSPQRTDDGAVRLVPAA"
     gene            2140..>2366
                     /locus_tag="VFX46_08575"
     CDS             2140..>2366
                     /locus_tag="VFX46_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX5600677.1"
                     /translation="MTKVKIGLALGCGAARGWAHIGVLHGLLEAGIVPDIVAGTSIGA
                     VVGGCYAAGRLNEVEEFARSLTRRRVFGYLD"
BASE COUNT          412 a          777 c          756 g          421 t
ORIGIN      
        1 actctcaacg tcattcggga gcctgtgccc gcaacgcgga gctgacggac gagctcccgg
       61 cgaccaaagc gccgagatga cggttcgatc actgcgccgc cacggcctga ttggcgcccg
      121 atcgccgcgc ttcgatctca tccagcgccc gttcaacggc atcgaacacg agaagggtgg
      181 acgcatgcct cgccttatag tccctcaccg gctccagaat ggcgaggtcc gcccaccggc
      241 ccgtcggcgg cgggccgttc tctttcagca tccgccgcat cgtccgcccg acctctcgca
      301 gctcgtccgg tgttgagccg atgatctcac gccccatgac cgatgcggcc gcctgcccca
      361 gcaggcacgc cttcaccgtc tggccgaaat ccgtgacccg gccggacgcg tccaccttca
      421 ggtcaacgac aaccgtcgag ccacacagct tcgaatgcgc ggtcgcggtg gcgtcaggat
      481 cgggaagcct gcgctgacgc ggaatctcgg ccgccagctc gaatatccgc gagttgtaga
      541 cgtcgtccag gccagccatc ccctgctccc gtcatccacc gccgcggcct agcggcccgg
      601 ttcccttgcg gcgtgagacc ctacactata tagcgagcga gagctgcagt gcgaggccca
      661 ggtgcgcggt ccagccacgc tgcgttatct tctcgtccgc tcaacccagt ggctcgttgc
      721 agaatcggaa cacggtcggt gacaaacatc tcagcagccc cgttcaaaaa caaggtgacg
      781 cacgcccgca ggcccaccaa acaggaggcc gaagaagccg tgcgcacgct catcgcatgg
      841 gccggtgacg acccggatcg cgagggcctg aaggaaacgc cgcgccgtgt cgtcgaggcg
      901 ttccaggagt acttccgcgg ctatggcgaa gacccggtag ccatgctcca gcagccgacg
      961 ttcgaggaga tcggcggcta tgatgacttg gtgatgctgc gcgacatccg cgtcgagagc
     1021 cactgcgagc accatatcgc gccgttcatc ggtgtggcgc acgtggcgta cctgccaaac
     1081 gggaaggtcg cggggctttc gaagctcgcc cgggttgtag aagcctacgc caagcggctg
     1141 cagacgcagg aaacgctgag cgcccagatc gccgatgcga tccaggaggc gctgtctccg
     1201 cgcggtgttg ccgtaatgat tgaggccgag caccagtgca tgaccatgcg cggcgttcat
     1261 cagcccggtg tcgccgctgt gaccacgcgc tttctcggtg cctttgccac cgactccgcc
     1321 ctgcgcgacc gctttcttag tctggcgaac gcgagccgac gcggccgctg agtccaccac
     1381 tgtgtcatcc cggcgctcgt cgccgggatc cacttatccg cttgctccgg ttttgtggat
     1441 ggttggatcc cgggcacaag gcccgggatg actctcagga agaacgtcgg ggtggtttta
     1501 aatggcttct ccatctcccc tcttcgcccc ccgcggctcg acagaggaaa tcgagcgcgg
     1561 cgacgttttc cagccgaagt tcgggccgga cggactaatc ccggcgatca tcaccgatgc
     1621 cgataccggt gacgttctca tgttcgcatg gatgaacgcc gaggcgctgg cgctcaccat
     1681 cgagacgggg ctggcgcatt tctggacgcg ctcgcggcag cgcatctgga agaagggcga
     1741 ggagagcggc aacctccttc gcgttcgaga aatccggacg gattgcgatc aggacgtgct
     1801 gtggctcaag gcgcacgtcg agggcaacgg cgtggcctgc catacgggcg cacgctcctg
     1861 cttctaccgg gcgctatctc cgcagcgtac ggacgatggc gccgttcgcc ttgtccccgc
     1921 cgcttaggct cgaggttaga cttagccgaa atttgaagtt gtgcgttgtt gataagcggg
     1981 tccgccggcg ctcccgtagg gctgggttaa tcctgtgcgg gttaccattc ttccggcgac
     2041 tctcaaccat aggtggcgac caaggtgtcc cgtctgcagt aatggacaca cgaggaacgc
     2101 caccaccccc gcaacggcaa cgacggcgga gatcgagcca tgacgaaagt gaagatcggc
     2161 ctggcattgg ggtgtggcgc ggcgcgcggg tgggcgcata tcggcgtcct tcacggactg
     2221 ctcgaagctg ggatcgtgcc ggacatcgtc gctggaacgt cgatcggcgc ggtcgtcggc
     2281 ggatgctatg cggccgggcg gctgaatgag gtcgaggagt tcgcgcgcag ccttacgcgg
     2341 cgccgggtgt tcggctatct cgactt
//
LOCUS       DASUMG010000517         3037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1114362, whole genome shotgun sequence.
ACCESSION   DASUMG010000517 DASUMG010000000
VERSION     DASUMG010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114362"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VFX46_08580"
     CDS             complement(<1..503)
                     /locus_tag="VFX46_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600678.1"
                     /translation="MVAVRDNAGERVESWWHKLQRTFAWLLRTVTAAFGDLLPKGLYA
                     RALIIIIAPIVLLESVVAFVFMERHWQAVTRRLSEATARDIAAIISVYREYPHRDDYA
                     RLIELAETMKLSMQLLPAGDLPPAQPKPFFSLLDRTLSNEIRKQIRQPFWVDTVGNSR
                     HVEIRVKL"
     gene            complement(590..1306)
                     /locus_tag="VFX46_08585"
     CDS             complement(590..1306)
                     /locus_tag="VFX46_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX5600679.1"
                     /translation="MTVEHGTIRRECLPDDAPHVLIVDDDQRIRDLLTRYLMEHGYRV
                     TPAADPSAARAAMRGLKFDLVILDVMMPGESGLDFARHLKATSDVPICMLTARSEPED
                     RIEGLEAGVDDYLPKPFEPRELVLRIKNILRRGKNNHANADEVRMGDLVFYIARGELK
                     RGDESIKLTERERDLLRQFAQRPGVAIARHELAGDDATGSERAIDVQINRLRRKIEAD
                     PSNPVYLQTVRSKGYILYTD"
     gene            complement(1337..1888)
                     /locus_tag="VFX46_08590"
     CDS             complement(1337..1888)
                     /locus_tag="VFX46_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX5600680.1"
                     /translation="MTDIYSPSRVGTAAPSHGEPATDLQKTVEREDLIASVELLFFAY
                     RDFTGEADAVLAEFGFGRAHHRVLHFVNRHPGLRVADLLDILKITKQSLARVLKQLVD
                     QGYILQKAGPEDRRERLLYLTGKGRELASKLLALQCARVAAALAEAGDESGTVTRRFL
                     AGMIAKGERPRIEKLIFGPDDRA"
     gene            2083..2976
                     /locus_tag="VFX46_08595"
     CDS             2083..2976
                     /locus_tag="VFX46_08595"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEX5600681.1"
                     /translation="MAPLVPYHDRDGFIWLDGKLVPWREANLHVLTHGLHYASAVFEG
                     ERAYGGEIFKLTEHTQRLFDGARTLDFEIPYTVEEIDAACRETVKANNLVDCYVRPIA
                     WRGSEQMGVSAQQTKIHVAIAAWDWGSYFDPKERLRGIRLAHAQYRRPDPRTAPAKTK
                     AAGLYMICTLEKHRAERAGYADALMLDWRGHVAECTGANIFFVKDGVLHTPVPDCFLD
                     GITRRTVIELAKKRGMEVQERTILPEELGSFSECFIVGTAAEVTPVREIGEHQYQPGR
                     ISETLLNDYMAAVQPKKSAAE"
BASE COUNT          597 a          918 c          931 g          591 t
ORIGIN      
        1 agcttcaccc tgatctcgac atgacgggag ttgccgaccg tatcgaccca aaacggctga
       61 cgaatttgct tccggatctc attggaaagt gtccggtcga gcagtgagaa gaacggcttc
      121 ggctgcgccg gcggcaggtc gcccgcggga agaagctgca tggacagctt catcgtttcc
      181 gctagctcga tgaggcgcgc gtaatcgtcc cgatgcgggt attcgcgata gacgctgata
      241 atcgccgcga tatcacgtgc ggtcgcttcg gacaggcgcc gggtcacggc ctgccaatgt
      301 ctctccatga acacgaacgc gacgacgctt tcgagcagta cgatcggcgc aatgatgatg
      361 atgagcgcgc gggcataaag tcctttggga aggagatcgc caaacgcggc cgtcacagta
      421 cggaggagcc aggcgaacgt cctttgcagc ttgtgccacc aggattcgac tctctcgccg
      481 gcattgtcgc ggactgcgac catcggtgcg gtgccctatc taagcccggg tagaggcatc
      541 ccgggccaaa gccgggatga tgcacgattt gaagcaacgg cctccacacc taatccgtat
      601 acaagatgta gcctttgctg cgcactgtct ggagatagac cgggttggac gggtcggcct
      661 cgatcttccg gcgcaggcga ttgatctgca cgtcgatcgc gcgctcgctt ccggttgcat
      721 catcgccggc gagctcgtgc cgagcaatgg caacacctgg ccgctgagcg aattggcgca
      781 gcaggtcccg ctcgcgctcc gttagcttga tgctctcgtc tccgcgcttg agctcgccgc
      841 gagcgatgta gaagacgaga tcgcccatgc gcacctcgtc ggcattggcg tgattgttct
      901 tgccgcggcg aagaatgttc ttgatgcgca ataccagctc acgcggttcg aagggcttcg
      961 gcagataatc atccacacca gcttcaagcc cctcgatgcg gtcctccggc tcggaacggg
     1021 ccgtcagcat gcagatcggg acatcggacg tcgccttaag gtgacgagcg aagtcgagcc
     1081 cgctctcccc gggcatcatc acatcgagaa tgacgagatc gaattttagc ccccgcatgg
     1141 cagcacgagc agcactgggg tcagccgcgg gggtcacgcg gtatccgtgc tccatgaggt
     1201 agcgtgtcag caagtctcga atgcgctggt cgtcatcgac gatgagcacg tgcggtgcat
     1261 catccggcag acattcgcgc cgaattgtcc cgtgctccac ggtcatgagt cccctcccgg
     1321 tcaaccgaaa cgaggctcag gccctatcgt ccggcccgaa aatcagcttc tcgatccgcg
     1381 gccgttctcc tttcgcaatc attccggcga gaaagcggcg cgtcacggtg ccggactcgt
     1441 cacccgcctc ggccagcgca gcagcaaccc gcgcgcattg aagcgccagg agcttgctcg
     1501 cgagctccct gcccttcccc gtcaagtaga gcaatcgctc gcggcgatcc tctggaccag
     1561 ccttctgcag aatgtagccc tgatccacga gctgcttcag cacgcgtgca agactttgct
     1621 tggtaatttt gaggatatca agaaggtcgg ccaccctcag tcctggatgg cgattgacga
     1681 agtgcagaac acggtgatgc gcccggccga agccgaactc agccagcacc gcatcggcct
     1741 cgccggtgaa gtcgcgatag gcgaagaaga gcagctcgac ggaagcaatg agatcctccc
     1801 gctcaacggt cttctggaga tcagttgcgg gctctccatg gcttggagcg gccgtgccta
     1861 cacgcgaggg tgaatatatg tcagtcatgt tgacttattt ggaggccatt gttaccggtg
     1921 gcaagtgcct ttgtgcactg tagaccgcgg gttgtgcggc tgccggcctc acagaagcaa
     1981 taaatcgcgc tctgatggga tggcgagtcg catccgcagc ctattgctgg catggcatcg
     2041 tcaccgaggc aggcagagat gacatctccg aggagataac cgatggctcc gctcgttccc
     2101 tatcacgacc gcgacggctt tatctggcta gacggcaagc tcgtgccatg gcgggaggcc
     2161 aatttgcacg tgctgaccca cggcttgcat tatgccagcg cggtatttga aggggagcgg
     2221 gcctatggcg gcgagatctt caagctgacg gagcacacgc agaggctatt cgacggcgcc
     2281 cgcactttgg atttcgaaat cccctacacg gttgaggaaa tcgatgcggc ctgccgcgag
     2341 accgtgaagg ccaacaatct ggtcgattgc tacgtccgcc cgatcgcctg gcgtggcagc
     2401 gagcagatgg gcgtctcggc ccagcagacg aagattcacg tcgccattgc tgcctgggac
     2461 tggggatcct atttcgatcc gaaggagcgg ctacgcggca ttcgccttgc acatgcccag
     2521 taccggcgcc cggatccgcg gacggccccc gcgaagacga aggcggctgg gttgtacatg
     2581 atctgcacgc tcgagaagca ccgcgccgag cgcgccggct atgcggacgc tctgatgctc
     2641 gactggcgcg gccacgtcgc ggagtgcaca ggcgccaaca tcttcttcgt caaggacggt
     2701 gttctacata cgccggtgcc ggactgtttc ctcgatggca ttacgcgccg cactgtgatc
     2761 gagctcgcca agaagcgtgg tatggaggtc caggagcgta cgatcctgcc cgaggaactc
     2821 ggctcgtttt cggagtgctt cattgtcggc accgctgccg aggtgacgcc agttcgcgag
     2881 atcggagagc atcaatatca gcctggccgc atctcggaaa cgctgctcaa tgactacatg
     2941 gcggctgtcc aaccgaaaaa gtcggccgca gagtagcgca attcgctatt tggcgccctt
     3001 tcttgagggc tatttggcag gtcggaggac aagctcc
//
LOCUS       DASUMG010000518         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_232176, whole genome shotgun sequence.
ACCESSION   DASUMG010000518 DASUMG010000000
VERSION     DASUMG010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232176"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VFX46_08600"
     CDS             <1..367
                     /locus_tag="VFX46_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase C-terminal domain-containing
                     protein"
                     /protein_id="HEX5600682.1"
                     /translation="VSKGETIGYNATWTAKHAARIAIVAVGYADGYPRAASASDAAPG
                     SDAIVADTRCPLAGRVSMDLLAVDVTGLAENAVRRGDFITLIGGTIGVDELAAAAGTI
                     GYEVLVNLGSRYHRIYRDS"
     gene            466..1872
                     /gene="radA"
                     /locus_tag="VFX46_08605"
     CDS             466..1872
                     /gene="radA"
                     /locus_tag="VFX46_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX5600683.1"
                     /translation="MSKRSLTFVCQNCGATFSRWQGKCEACGEWNTLSEEGAERPSGP
                     GRRPGKGRLFALEALTGEAHDTPRLVSGIGELDRVTGGGFVRGSVLLVGGDPGIGKST
                     LLIQAAAALARAGHRAVYISGEEAVAQVRLRAERLGLRDAPVELAAETSVGDIIATLS
                     EGKVPRLIVIDSIQTMWTDMVESAPGTVTQVRGSAQALIRFAKKTGAAIILVGHVTKD
                     GQIAGPRVVEHMVDAVLSFEGEGSHQFRILRAVKNRFGPTDEIGVFEMTGSGLREVGN
                     PSELFLSERDLGQPGTAVFAGIEGTRPLLVEIQALVAPTSLGTPRRAVVGWDPSRLSM
                     VLAVLEAHCGVRLGGHDVYLNVAGGMRIHEPAGDLAAAAALVSSLANAPLPADAVFFG
                     EVSLSGAVRPVAQSAARLKEAAKLGFTRAIVPDAARSEAAEPSLKVTDIDSLAGLVAD
                     IAARGKTPRLVKTGGQDG"
BASE COUNT          393 a          628 c          673 g          407 t
ORIGIN      
        1 cgtttcgaag ggcgagacta tcggctacaa tgcgacctgg actgccaagc acgcagcgcg
       61 cattgccatc gttgcggtcg gctatgccga cggatatccg cgcgcagcca gcgcctccga
      121 cgcggctccg gggtccgatg ccatcgttgc tgacacgcgc tgcccactgg ccgggcgcgt
      181 gtcgatggat ctgctggcgg ttgacgtcac ggggcttgcc gaaaatgcag tgcgtcgcgg
      241 cgatttcatc accctcatcg gaggcacgat tggggtagat gaattggcgg cagccgcagg
      301 aacgatcgga tatgaggttc tggtcaatct gggcagccgg taccaccgca tataccgcga
      361 tagctgaccg caaatcccag caaattagta ccttcttaaa aaatgttctt gtattgttct
      421 cacggtcgct ctaccgtcgt gaacggccat ttgcggcgat tccccatgtc caagcgcagc
      481 ctgacattcg tctgccaaaa ctgcggcgca actttcagtc gctggcaggg caaatgcgag
      541 gcctgcggcg agtggaatac gctcagtgaa gaaggggctg aacgaccttc cgggccgggt
      601 cgcaggccag gcaaaggtcg gctgtttgca ctcgaggcat tgacgggcga ggcccatgat
      661 actccccgac tggtgtcggg gattggcgaa ctcgaccggg tcaccggggg cggttttgtt
      721 cgcggttcgg tgctcctggt cggcggcgac cccgggatcg gaaagtcaac tttgctgata
      781 caagcagccg ccgcgcttgc gcgcgcaggt caccgagccg tctatatctc cggcgaggaa
      841 gcagtggctc aagtccgtct gcgggccgag cgcctcggcc tgcgtgatgc gcctgtcgag
      901 ctggccgctg agacatcggt cggagatatc atcgcgacgt tatcggaagg gaaagttccg
      961 cgccttatcg ttatcgattc aatccagacc atgtggaccg acatggtcga atccgccccg
     1021 ggaacggtga ctcaagttcg tggcagcgcg caggcattga ttcgctttgc aaaaaagacc
     1081 ggcgcggcaa ttattcttgt cggccacgtc accaaggacg gacaaatcgc aggcccgcgc
     1141 gtcgtcgaac acatggtcga tgcagtgctg tcgttcgagg gcgaaggttc gcaccaattc
     1201 cgtatcctgc gcgcggtcaa aaaccggttc gggccgacag atgaaattgg cgtttttgaa
     1261 atgaccggtt cgggcctgcg ggaggtcggc aatccatccg agctgttctt gtcagagcgc
     1321 gatctcggcc agcccggcac ggccgtcttc gcgggcatcg agggaacgag accgttgctg
     1381 gtcgagattc aggcgcttgt cgcgccgacc tcgctcggga cgccacgacg cgctgtcgtc
     1441 ggctgggatc ccagccgtct gtcgatggtg ctggcggtgc ttgaggccca ttgtggggtg
     1501 cggctgggag gacatgacgt ttacctcaac gtagcgggag gcatgcgtat tcacgagcct
     1561 gctggtgatc ttgcggccgc cgctgcactt gtgtcgtcgc ttgcaaacgc tccacttcct
     1621 gcagacgcgg tcttttttgg agaagtgtcg ctgtccggtg cggtacgtcc ggtggcgcaa
     1681 agcgccgcca gactcaagga agcggccaaa ctcggattca ctcgcgccat tgttcctgac
     1741 gctgcgcgca gcgaggcggc ggagccgagc cttaaggtta ccgacatcga cagtcttgcg
     1801 ggactcgtcg cggacatcgc ggctcgcggt aaaacacccc gacttgtcaa aactggtgga
     1861 caagacgggt gacggcgccc cggcgccccg ctatatacgg catcaaaacc ggttccggcg
     1921 gcccgtaaag gtacgctgcc gccagcgcag aaatgggcta aaaattaagc acttaaggtc
     1981 gacattgccg cccctgcccg gagccgctat ggctggcccg gattcgtcgc cctggcccta
     2041 ccctaggcgc tacggcaaac gcctgagcac gcggagcgcc tgagcaaccg atgccgatca
     2101 c
//
LOCUS       DASUMG010000519         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_632136, whole genome shotgun sequence.
ACCESSION   DASUMG010000519 DASUMG010000000
VERSION     DASUMG010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632136"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VFX46_08610"
     CDS             <1..640
                     /locus_tag="VFX46_08610"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017487513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX5600684.1"
                     /translation="ASQNKAEAAKKAGKFKDEIAPVTIVTRKGETVVSEDEYIREGTT
                     YEAVAKLRPAFEKDGTVTAGNASGINDGAAAVVLMTLEEAEKRGITPLARIVSWAHAG
                     VDPSIMGTGPIPASKKALAKAGWTVDDLDLIEANEAFAAQACAVNKGLGWDTEKVNVN
                     GGAIAIGHPIGASGARILTTLLYEMKRRDAKKGLATLCIGGGMGVAMCVERI"
     gene            771..1496
                     /gene="phbB"
                     /locus_tag="VFX46_08615"
     CDS             771..1496
                     /gene="phbB"
                     /locus_tag="VFX46_08615"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEX5600685.1"
                     /translation="MARVAVVTGGTRGIGAAISVALKNKGYRVAANYAGNDAAARQFQ
                     SEHGIPVFKFDVGDYAACEQGVRQISSELGPIDVLVNNAGITRDSMLHRMTKENWDDV
                     IRTNLDSVFNMTRLVIEGMRARNFGRIISISSINGRKGQIGQANYSAAKAGLLGFTKA
                     VAQEGASRGITCNVIAPGYISTEMVAAVPKEVLETKILPSIPVGRLGTPEEVARCVVF
                     LAADEAGFITGACLDANGGQYMA"
     gene            complement(1511..>2308)
                     /locus_tag="VFX46_08620"
     CDS             complement(1511..>2308)
                     /locus_tag="VFX46_08620"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX5600686.1"
                     /translation="RMRLWGSLAFIVATFVGGALVESLGATSIIWMLVACSGGLLASA
                     WLLPVNHPGAAGERPVTPGRRLLTPRDVARVAASWRFLLFVAAAGLVQASHAVFYTFG
                     VLHWQAQGLSSITIGVLWSLGVLAEILLFFSAGRFMALIGPIGFILAGAVAAVVRWGL
                     MAFDPPLALLFPLQVLHGLTFGATHIGAMHHIHAVVPQEQAGTAQALFAAATGGIGMG
                     AATLLAGMLYGPFGGLSYLAMMAMGVIGLIAAVGTGLSSPTHKPDHS"
BASE COUNT          494 a          730 c          734 g          350 t
ORIGIN      
        1 cgcctcgcag aacaaggccg aggctgccaa gaaggcgggc aagttcaagg acgagatcgc
       61 tcccgtaacg atcgtaaccc ggaaaggcga gacggtcgtc tccgaggacg agtacatccg
      121 cgagggtacc acctacgaag ccgtcgccaa gctgcgcccg gcgttcgaga aggacggcac
      181 ggtgacggcc ggcaatgcct cgggcatcaa cgacggcgcc gccgctgtcg tgctgatgac
      241 gctggaagaa gcggagaagc ggggcatcac gccgctcgca cgcatcgtct cctgggccca
      301 tgccggcgtt gatccgtcca tcatgggtac cggtccaatc cccgcctcca agaaggctct
      361 ggccaaggcc ggctggaccg tcgacgatct cgacctcatc gaagccaacg aggccttcgc
      421 ggctcaggct tgcgccgtca acaaggggct cggctgggat acggagaagg tcaacgtgaa
      481 cggtggcgcc atcgcgatcg gccaccccat tggcgcgtcc ggcgcgcgga tcctcaccac
      541 gctcctctac gagatgaagc ggcgggatgc gaaaaagggc ctcgccaccc tctgcatcgg
      601 cggcggcatg ggcgtagcga tgtgcgtcga acggatttga tcgcgcgcag agactggaaa
      661 ggatctgatc aataaagggc tggtgaagtc atagccagcc cttgcaatat gcgacttcgg
      721 tccgacatca aacgatcagg tcttcgcgcg ttcatatctg ggaggcaaga atggcacgtg
      781 tggcggtcgt caccggcggc acgcgcggca tcggtgccgc gatctccgtc gcgctcaaga
      841 acaagggata ccgcgtagca gcgaattacg caggcaacga tgcggccgcc cgtcaattcc
      901 agagcgagca cggcatcccg gtattcaagt tcgacgttgg cgactacgcg gcctgcgagc
      961 agggcgttcg gcagatctcg agcgagctcg gtccgatcga cgtgctggtg aacaacgccg
     1021 gcatcacgcg tgactccatg ctacaccgga tgaccaagga gaattgggac gacgtgatcc
     1081 ggaccaatct cgattccgtc ttcaacatga cccgtctcgt gatcgagggc atgcgggcgc
     1141 gcaacttcgg acgaatcatc tcgatctcct cgatcaacgg gcgaaagggc cagattggtc
     1201 aggccaatta ttcggcggcc aaggccgggc tgttgggatt caccaaggca gtcgcgcaag
     1261 agggtgcctc cagaggcatc acctgcaatg tcatcgcacc ggggtacatc agcacggaaa
     1321 tggtcgctgc cgtgcccaag gaggtgcttg agaccaaaat cctgccgtcc atcccggtag
     1381 gccggctcgg cacaccggag gaggtggctc gctgtgtggt cttcctcgct gccgacgaag
     1441 ctgggttcat cacaggcgcc tgcctcgacg ccaacggtgg tcaatacatg gcgtgagccg
     1501 aaacggctcc tcaagagtgg tccggtttat gcgtcgggga ggaaagtccg gtgccgacgg
     1561 cagcgatcag cccgatgacg cccattgcca tcatggcgag gtagctcaag ccgccgaacg
     1621 gcccatagag catgccggcc aggagcgttg cagcgcccat gccgattccg cccgtagctg
     1681 ccgcaaacag ggcttgtgcc gttccagcct gctcttgcgg gactacggcg tggatatgat
     1741 gcatggcgcc gatatgcgtg gcaccaaatg tcagcccgtg cagcacctgt agcggaaaga
     1801 gcagcgccag cggcgggtcg aaagccatga gaccccatcg gaccacggca gcaacggccc
     1861 cggccagaat aaagccgatc ggcccgatga gggccatgaa acgccctgcg gagaagaaga
     1921 gcaggatttc ggcgagcacg ccgagcgacc agagcacccc gatcgtgatg gacgagagcc
     1981 cttgagcctg ccagtgcaga acgccgaacg tatagaagac tgcatggctc gcctgcacca
     2041 gtcctgccgc agcgacgaac aacagaaatc tccatgaggc cgcgaccctc gcgacgtctc
     2101 tcggagtaag gagacgtcgc cctggcgtga caggccgctc ccccgcggcg cctgggtgat
     2161 tgaccggcag cagccacgcc gacgccagca gaccgcccga gcaggccacc agcatccaaa
     2221 tgatcgacgt cgctcccaga ctttcgacca gcgcgccgcc cacgaaagtc gcaacaatga
     2281 aggcgagcga cccccagagc cgcattcg
//
LOCUS       DASUMG010000520         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_70834, whole genome shotgun sequence.
ACCESSION   DASUMG010000520 DASUMG010000000
VERSION     DASUMG010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70834"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFX46_08625"
     CDS             <1..904
                     /locus_tag="VFX46_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEX5600687.1"
                     /translation="VAPDRLRFDFSHPKPMTAEEVRKVEDLANSIVAQNVPVETRLMS
                     LEEAMESGAMALFGEKYGDEVRVVSMGLAPAGSNRPWSMELCGGTHVKRTGDIRIIRI
                     VSEGASAAGVRRIEALTADGARAYLNQQDDRVREAANVLRVRPEELLDRVKALMEERR
                     TLERQLADAKRQLALGGGGNGVAAADGIRTVGNIKLLARTVEGLNPKDLRGLVDDGKK
                     KVGSGVVAIVGVTEDGKAGIAVGVTDDLTKTYNAVDLVRAGAEVLGGKGGGGRPDMAQ
                     AGGPDGSKAQLALEVIERRLSGAA"
     gene            1069..1392
                     /locus_tag="VFX46_08630"
     CDS             1069..1392
                     /locus_tag="VFX46_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600688.1"
                     /translation="MRLSLPKFAQSKHLRRAGMPVGILLAVFLSGCASSSEYGEYWIG
                     GERIAMEADGLPAQLPPPHHRKPDVDDPREPFSPNYGPPPQPSLPADLPPAFRRRLIN
                     AVSIS"
     gene            1550..1906
                     /locus_tag="VFX46_08635"
     CDS             1550..1906
                     /locus_tag="VFX46_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600689.1"
                     /translation="MKMRLLSLAAAAALLIVATPGPEARAAAAKLPVLKSEASDIVEV
                     RHRKKLRRKFYKRRYHRRGYYYPRRYHRHYHRRWRGPRFAIYVGSPRCSWLRRRAILT
                     GSRYWWRRYYRCRYWW"
     gene            complement(1964..3109)
                     /locus_tag="VFX46_08640"
     CDS             complement(1964..3109)
                     /locus_tag="VFX46_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HEX5600690.1"
                     /translation="MSAAENEPRIYRGLKDVYFERSPTTFIDGKAGELRYRGYSIHDL
                     AEHSTFEETAYLLLYGELPTRSELAKFDEDLKAARQLPPAVLDIISAVKDAHPMDVLR
                     TAVSALAAFDPETTDNSREATLRKGIRLSSQVPMIVAAHEQIRNGRAPIAPDPQLSHA
                     ANFLWMLKGEKPSQDAARLLDTDLVLHAEHGSNASSFTARVVISTEAHIHAAITAAVA
                     ALSGPAHGGAAEDVMRMAQEIGDPANVPAYVKAKRAAKEPITGFGHRVYRAEDPRARH
                     MREGVKRLSKEMGEPRWYEILEALVKEMEPYARHGVNVNVDFYSGVIYHLHGIPNDLF
                     VPIFAVGRVPGWTVQVLEQQANNILIRPLTLYNGPGPRDYVSIDQRG"
     gene            3199..4185
                     /gene="pta"
                     /locus_tag="VFX46_08645"
     CDS             3199..4185
                     /gene="pta"
                     /locus_tag="VFX46_08645"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEX5600691.1"
                     /translation="MDIIADCMAAIRSRGLKVILPESDDERVLRAARLLLDEDLARPV
                     LLGVPESIESRAANLELSLDGLEIRNPDGDPDLEGLAAEIASGRERMTTSMALRLLRR
                     PLYYGGALVASGQAAAMVAGAANPTKRVIEAGLMTVGLAEGVATPSSFFLMLLPATHD
                     GAPRAYVFADCAVNADPDASMLADIAIASAASAEALLRQEARVAMLSFSTHGSANHPH
                     VEKVRAAVDIVRERKPDLKIDGELQADAALVGSVAAKKVKGESAVAGRANVLVFPDLD
                     AGNIAYKLVQYMADAQAIGPFLQGFAKPIADLSRGASVDDIVATAAVTLARA"
     gene            complement(4414..>4619)
                     /locus_tag="VFX46_08650"
     CDS             complement(4414..>4619)
                     /locus_tag="VFX46_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600692.1"
                     /translation="IDSALDFVSTGGKTDRPRGVVQAIRDFFASLRSGIDAAHHYERL
                     KAQGVPPHEAVEIVFRDHFENKN"
BASE COUNT          838 a         1392 c         1470 g          919 t
ORIGIN      
        1 ggtcgctccg gatcgcctac gtttcgactt ctcgcatcca aagcccatga cggctgagga
       61 ggtaagaaag gtcgaggatc tggcgaattc aatcgtggct cagaacgtgc ccgtggaaac
      121 ccgcctgatg tcgctcgaag aggcgatgga aagcggcgcc atggctctct ttggcgagaa
      181 gtacggggac gaggttcgcg tcgtgtccat gggattggcg cccgctggct ccaaccggcc
      241 ctggtccatg gagctatgcg gcggcacgca cgtgaagcgc accggcgaca tccgcatcat
      301 tcgcatcgtc tcggaaggtg catctgctgc cggcgtgcgc cgtatcgagg cgctgaccgc
      361 cgatggtgca cgcgcttacc tcaatcaaca ggacgatcgc gtgcgcgagg cggcaaacgt
      421 gctccgcgtg cggcccgagg agttgctcga ccgcgtcaag gcgctgatgg aggaacggcg
      481 gacgctggag cgtcagctgg ctgatgcgaa acggcagctc gcgctgggcg ggggtggcaa
      541 tggtgtcgcc gctgcggacg gcatccgcac cgtcggcaac atcaaactgc tcgctcggac
      601 cgttgagggt ctgaacccga aggatctccg cggcctcgtc gacgacggga agaagaaggt
      661 cggctcgggc gtggtcgcca ttgttggcgt gaccgaggat ggcaaggccg gcattgccgt
      721 aggcgtgacg gatgatctga cgaagaccta caacgccgtc gatctcgtcc gtgctggcgc
      781 tgaggtactg ggcggcaaag gcggtggcgg tcgcccggac atggcccagg ctggtggccc
      841 ggacggcagc aaggcccagc ttgcgctgga ggtcatcgaa cggcggctga gcggagccgc
      901 gtaaccgcgg cccgttgcac cgattgcgtc cgtgccgccc aatgcctggc gtcgagtcga
      961 ccgctaactc gatctcgaaa tgtattgaaa taggacggcg actaaccggg ccttaaccgt
     1021 gcctgggctc tgatgttcga tagtatcgtg tcgattggag ttcgggtaat gcgtttgtcg
     1081 ttgccgaagt tcgcgcagag caaacacctg cgccgcgccg ggatgcccgt tggcattctt
     1141 ctcgcagttt tcctttccgg ctgtgcaagc tcatccgagt acggcgaata ttggattggc
     1201 ggtgaacgta tcgccatgga ggcagacgga cttcctgccc aacttccgcc accccatcat
     1261 cggaaaccgg atgtagacga cccccgtgag ccgttcagcc cgaattacgg gccaccgcct
     1321 cagccgtcgt tgcccgctga cttgccaccg gcattccgtc gcaggcttat caatgcggta
     1381 agtatttcgt gattttgact ggcgtcaatg cggatgcaac tgaaactcgg gttggatctc
     1441 ggggttatcc gtattagggt actgcgatca cgtggggaag ctcagcaaga cagtggtttg
     1501 tcgtcggccg aaatcctgtg ccggcgcgca gctgttcagg agtctgaaaa tgaagatgcg
     1561 tctgctttcc ctagcggctg ctgccgccct cctcatcgtc gccacaccag ggccggaagc
     1621 gcgcgctgca gccgctaagc ttcctgtgct taaatccgag gcttcggata tcgttgaagt
     1681 ccggcatcgg aagaaattgc gccgaaagtt ttacaagcgt cgttaccacc gccgtggcta
     1741 ctactacccg cgccgctacc accgccacta tcaccgccgc tggcgtggcc ctcggtttgc
     1801 gatctacgtc ggctcaccgc ggtgctcgtg gttgcgtcgc cgggctatac tgaccggcag
     1861 ccggtattgg tggcgtcggt actaccgctg ccgttactgg tggtaattgc gaatgcaaac
     1921 cgggccgaat tcttcggccc ggttttctct tcacgggcag ctttcatccg cgctggtcaa
     1981 tactgacgta gtcccgtggg ccggggccgt tgtagagcgt cagtggccgg atgagaatgt
     2041 tgttggcctg ctgctccaat acctgaaccg tccacccggg cacgcggccg accgcgaaga
     2101 tcggcacgaa cagatcgttc ggaatgccgt gcaggtgata gataacgcct gagtagaagt
     2161 cgacattcac gttgacgccg tggcgggcgt aaggctccat ttcctttacg agcgcttcca
     2221 ggatctcata ccaccgcggc tcgcccattt ccttcgacag ccgcttgacg ccttcgcgca
     2281 tgtgacgcgc cctcggatcc tcggcgcgat aaacgcggtg tccgaagccg gtgatcggct
     2341 cctttgccgc gcgtttcgcc ttgacatagg ctggcacgtt ggccggatcg ccgatttcct
     2401 gcgccatcct catcacgtcc tcggcagccc cgccgtgagc cggacctgaa agtgctgcga
     2461 cggccgccgt tatggcggcg tggatgtggg cctcggtgct gatcacgacg cgcgcagtga
     2521 atgaagaggc gttcgagcca tgctcggcgt gcagcaccaa atccgtgtcg agcaaccgcg
     2581 ccgcatcttg cgagggcttt tcgcccttga gcatccacag gaagttggcg gcgtgcgaca
     2641 gctgcgggtc gggcgcgatc ggggcgcggc cattccggat ctgctcatgc gcggcgacaa
     2701 tcatcggcac ttgggagctc agtcgtatgc ccttgcgcaa ggtcgcctcg cggctgttgt
     2761 ccgtcgtttc cggatcgaat gctgcaagcg ccgaaacagc ggtgcgcaga acatccatcg
     2821 gatgggcatc cttcacggcg gaaatgatgt ccagcacagc cggtgggagt tgacgcgccg
     2881 ccttgaggtc ttcatcgaac ttggcaagct cgcttcgagt cggcagctca ccatagagca
     2941 gcaggtacgc agtctcctcg aacgtcgaat gctcagcgag gtcatggatg gaatagccgc
     3001 ggtagcgcag ctcacctgcc ttgccgtcga tgaatgtcgt gggggaacgc tcgaagtaga
     3061 cgtccttcaa accacggtag attctgggct cgttctctgc cgcgctcata tgcttgaacc
     3121 tccattccgg aattgcgatg ccagtgagga tgccggcgtc tgccaactgc ctgccaaaga
     3181 attttcgaga gggcctacat ggacatcatc gccgattgca tggccgcgat cagaagccgc
     3241 gggctgaagg tgatccttcc cgaaagcgac gatgagcgtg tgctgcgagc cgcgcgcctt
     3301 ctgctcgatg aggacttggc gcgccctgtg ctcctgggcg taccggaatc gattgaaagc
     3361 agggcggcca atctcgagct gtccctggac ggtctcgaaa tccgcaatcc cgatggagat
     3421 ccggatctcg aaggacttgc tgccgaaatt gcctccggcc gggaacgaat gacaacgagc
     3481 atggccctgc gattgctccg tcggccgctc tactacggcg gcgccttggt tgcctcgggc
     3541 caagcagcgg ccatggtggc cggcgccgcc aatccaacga aacgtgtcat cgaggcaggg
     3601 cttatgacag tcgggcttgc ggagggtgtc gccaccccgt cgagcttctt tctcatgctg
     3661 ctgcccgcaa ctcacgacgg cgctccgcgg gcctatgtct tcgccgattg cgccgtgaac
     3721 gcggatcccg atgccagcat gctggccgac atcgcgatcg cttccgcagc cagtgccgag
     3781 gcgctcctgc gtcaggaggc gagagtggcg atgctgtcct tctccaccca cggtagcgcc
     3841 aaccatccgc atgtcgagaa ggttcgcgcg gcggtagaca ttgttcggga gcggaagccc
     3901 gacctgaaaa ttgacgggga gctgcaggcg gatgcggcgc tggttgggtc ggttgctgcg
     3961 aagaaggtca aaggggagag tgccgtagct ggccgcgcga acgtgctcgt cttcccagat
     4021 ctggacgcag gcaatatcgc ctacaagctc gttcaatata tggctgacgc ccaggccatc
     4081 ggacctttct tgcagggatt cgccaagccc atcgctgatc tctcgcgcgg tgcgagcgtc
     4141 gacgacatcg tcgcgacagc cgccgtcacc ctcgctcggg cctgagcgcc gatgctgcga
     4201 gatcggttcg gcaggcctgt ctgcaaccac gctctgaact cgagtggcgc ccatcgatct
     4261 aaaatacggg acaagttgtc tcgaataccg gattgcatct agaaagactc tggatgcccc
     4321 tgtcaatgcc gatgggctca tcacaaaata aaaacgccgt gcgggctgca cggcgcgagg
     4381 agtttggggt ctagtctggg gtactgttcg cgatcagttc ttgttctcga agtgatcgcg
     4441 gaagacgatt tcgaccgcct cgtgcggagg tacaccttgc gccttgagcc gctcgtaatg
     4501 gtgagctgcg tcaatgccgc tgcgaagcga cgcgaaaaag tcccggatcg cctgtaccac
     4561 accgcgcggg cggtcggtct tcccgcccgt ggacacaaag tccaaggcag agtcgattg
//
LOCUS       DASUMG010000521         3697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_70848, whole genome shotgun sequence.
ACCESSION   DASUMG010000521 DASUMG010000000
VERSION     DASUMG010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70848"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..841)
                     /locus_tag="VFX46_08655"
     CDS             complement(239..841)
                     /locus_tag="VFX46_08655"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX5600693.1"
                     /translation="MDYEFLVETYGSERLKTLSVWSMFADDDLNVRPHPRLDRDRTPR
                     EHMVHQCMSEDRWFCGMFGIDVAAPPLPADETRLAFIQRYAEDSGKRLARLREQDNAW
                     WAETVAFFDTTHSRAWILVRRIAHTAHHRAEQTTLLRLLGRRVHSVYGPSIDTGGLPA
                     NNAPTIYAYPTIESLIAGEMRGGAKASLPGPGDKPSTERA"
     gene            complement(1723..1890)
                     /locus_tag="VFX46_08660"
     CDS             complement(1723..1890)
                     /locus_tag="VFX46_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600694.1"
                     /translation="MEYVNGQFFRGLRIVGDPHNEREDDPVQLAVQSLQGAVVSLGDP
                     LQESRPLAFWD"
     gene            2280..2852
                     /locus_tag="VFX46_08665"
     CDS             2280..2852
                     /locus_tag="VFX46_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX5600695.1"
                     /translation="MSAASKRHDRHHEELVALYLLQALPPEERLELEAAIGACPTCQD
                     EVETLKPVVEHFVEWPTDLLRPSAGLWDRLARRIGIESAEEAAMPSPVALGWQEAGPG
                     ISYNVLSMDRARNRVSLLVRLEPGAAYPPHTHAGIEELYLLDGELWIDSRKVFPGEYY
                     RAESGSADERVWSETGCTCVLLTSSEDLLH"
     gene            3389..>3697
                     /locus_tag="VFX46_08670"
     CDS             3389..>3697
                     /locus_tag="VFX46_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5600696.1"
                     /translation="MAVIVLVVDDDPDARYIVSQFIRSQQGVDAVLHAADGVEAIEIM
                     RRESPRVVITDLMMPRLDGLQTTRLIREEWPDTRIVVITSLGDKSYEQDAYAYGADAF
                     L"
BASE COUNT          695 a         1125 c         1182 g          695 t
ORIGIN      
        1 cctcgcgacg cgcaccgctg aagggatcgt ggccgcgttc caaagaggag agttgtaaga
       61 cgcgactgaa cgcagccggg cgctcgagcg gaaagtcgag gttcttgggc catgcgagcg
      121 atgcgcaggg agaagagcgg aggcgtccag cagttcagag cgacgagtca tgcgcgccat
      181 cgccggggag acttgtcagg gttcggccat gttgatagtc gccgtcatgt cgaagggttc
      241 aggcccgctc ggtactcggc ttatcgccag gtccgggaag ggatgctttc gcgcccccgc
      301 gcatttctcc ggcgatcaac gactcgatgg tcggatacgc atagatcgtc ggcgcgttgt
      361 tcgccggaag cccaccggtg tcgatcgacg gcccgtagac actgtgcaca cgccggccga
      421 gcaatcgcaa caacgtcgtc tgctcggctc gatgatgcgc ggtgtgcgcg atgcgtcgga
      481 ccaggatcca cgcgcgcgaa tgcgtggtgt cgaagaacgc cacggtttcg gcccaccacg
      541 cgttgtcctg ttcccggagt ctcgcgaggc gcttgccgga atcttcggca taccgctgga
      601 taaacgcgag ccgcgtctcg tccgccggca agggcggcgc cgcgacgtcg ataccgaaca
      661 tgccgcagaa ccacctgtcc tcgctcatgc actgatgaac catgtgctcg cgcggcgtcc
      721 gatctcgatc gagacgtgga tgcgggcgaa cgtttagatc gtcgtcggcg aacatgctcc
      781 atacgctgag cgtcttcagc cgctcgctgc cgtaggtctc gaccaggaac tcgtagtcca
      841 ttcgtccccc ctcaccgtcg cgccgacgcg catcgagtgc cgatagacct agactttacg
      901 cggcgatcaa ttcctgaacg ctttccacga ggtccctgcg ccgccgtgcc tggctcttcc
      961 cccgcggaga gatgtgctaa aaagagaaac acggcgcccg gtggtgcgtc ttgcagggtc
     1021 ctctttaata ctgccaagct ccggatgcgt tgagtgcccc ggcagcctcg tcgtcacggc
     1081 tcagggtgag cgcgctggcg gcctggtagc gtgacttccg ggacatcggg ggcgcatgct
     1141 cgcgctttca gggacggctg gcaccagccg ctacaggtat gtggcaagcc gagagctcga
     1201 aagtctcgcg gagtccgctt ccgtagccag gtgctggctc caggcgcaag cgtccagtgc
     1261 ggcagtcagc aatccagtgc gctggctttg tcgttttatt gggcgatgga ccaaatggct
     1321 cgggccgacg ccgacggggt ggatccctcc ccgccccggc acgagatcct aactcgcaag
     1381 ctgccccacc gtcgtccgga ccgcatctgg gggggcccaa gcttcggggg agactgtgcg
     1441 atttgtgatc aaccgctcag acacggtgat ctcgaaatcg agatggagtt taagagaatt
     1501 gcagcggggg aatgtgatcc cgcccggtat cgggctcacg tgccgtgctt ttccggatgg
     1561 gcctcggaga tctctgaagc agtcaatgaa gaggtctcaa gggcgcccga gtattgaccc
     1621 tgttcttcgc gccccatata ttccctgctc atggggcgac cgcctacgca gggggggcag
     1681 ccaaagaccc tcggagacct gctgtttgcg agaggcccca actcagtccc agaaagcgag
     1741 tgggcgggac tcttgcagcg gatcgccgag ggagaccacc gcgccctgca ggctctgtac
     1801 agccagttgc accggatcgt cttcacgctc gttgtgcgga tcacccacaa tccggagacc
     1861 gcggaagaat tgaccattga cgtattccat ggagtctggc gccgggcgtc tacctacaca
     1921 ccggagggag gatctgtcgt cggttggatc atgaaccaag cccggtcgag ggcgatcgat
     1981 cgcctgagat ttgagcatcg caagaaacgt gtcgcacccc cgttcgttga gcctcagggc
     2041 gagagccccg agtcagatcc gcatcaccaa gcgacggttc gcgagcacag gcggcggtta
     2101 cgtgacgcac tggcggattt atcgcaggag gagcggcgcg caatcgagac cgcattcttt
     2161 tctgagttaa cgtatgcgga ggcggcgtcc cgattgaacg aaccggtcgg cacgataaag
     2221 acgcgaatcc gctctggact gagcaagccc agacgagcgc tcgcggaaaa tagtcttcta
     2281 tgagcgcagc atcgaaacgc cacgaccgcc atcacgaaga gctcgtcgcg ctctatctgc
     2341 tgcaggcgct gccccccgag gagcggcttg aactagaagc ggcgatcggg gcttgcccga
     2401 catgccagga tgaagtggaa acgctaaagc cggtagtcga gcattttgtc gagtggccga
     2461 cagacctctt acggccgtcg gcggggctgt gggaccgcct cgctcgccgt atcggaattg
     2521 agtctgctga agaggcagca atgccatcgc ccgttgccct cggttggcag gaggcgggtc
     2581 cgggtatttc ctataacgtg ctttcaatgg accgggcacg aaatcgggtc agtttgttgg
     2641 tgaggcttga gccgggcgcg gcctatccgc cccacaccca cgctgggatc gaggagctct
     2701 atctgctgga cggcgaactc tggatcgaca gccgaaaggt ctttccgggt gagtactatc
     2761 gcgcagagtc cggcagcgcg gatgaacggg tttggagtga aacgggctgc acgtgcgttc
     2821 tcctcacttc cagcgaggat cttctccact agctagccga ccgcctcttg ttggccgcct
     2881 cgcgggctag gccgccagcc cgatcacctc gagggcgtta cttcccatcc ttcgcggagt
     2941 ccttggctct cgtcagctcg ctccccaagc ggacgaactc ctcgcggtcc ataggcttac
     3001 gagcgcgcgg gaacatttcc gcttcttcct catccgcatg aacttcgcgc gctcgatctc
     3061 tggaagaacc aggtcaacga cctgatgctt ctcgggtgag cactcggcat gcccgctgcc
     3121 ggcccgccgc ctcggggcaa ctcgccgaga gaagcgctgc ccgcttctca accctggcat
     3181 cgggattgcc tttgccgaac gtcgtttaag agcaatggag tgagacgaac ctgacgggtg
     3241 tgttctcaat gaggacgcct taccaacatg ggtgtgacgg cgctggtttg cggccgacga
     3301 ggcacacgcg aggcggactg ggaaagaacg accccgtgag taaaagggtg cgctctctcg
     3361 cgctgccggc tcgggaagcg gagcctccat ggcagtgata gtgctggtag tggacgacga
     3421 tccggatgcc cgctacattg tttcccaatt catccgatcg caacagggtg tggacgcggt
     3481 tcttcacgcg gcagatggtg tcgaggccat cgagatcatg cggcgagaaa gcccgcgagt
     3541 cgtcatcacc gatttgatga tgccccggct cgacggacta cagacgacgc gactcatcag
     3601 ggaggagtgg cccgacacgc ggatcgtggt gatcacgtcg ctcggagaca agtcgtatga
     3661 gcaagacgcc tatgcgtacg gcgcagatgc gttcctc
//
LOCUS       DASUMG010000522         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_953918, whole genome shotgun sequence.
ACCESSION   DASUMG010000522 DASUMG010000000
VERSION     DASUMG010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953918"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..922)
                     /gene="purN"
                     /locus_tag="VFX46_08675"
     CDS             complement(260..922)
                     /gene="purN"
                     /locus_tag="VFX46_08675"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEX5600697.1"
                     /translation="MSKKRVAILISGRGSNMMSLVEAARDPSYPAEIILVISNRPEAP
                     GIAWAASQGLPTKVIDHKSYPSREDFEAALHEALVASGAEIVCCAGFMRLMTAGFVEK
                     WSGRMLNIHPSLLPAFKGLDAQAQALAAGVKIAGCTVHFVEPEMDSGPIIAQAAVPVS
                     DDDTPDTLAARILSAEHKLYPHALRLVAAGQVRLEGRRVVVDGAVNQTAQLFSPSLEY
                     KG"
     gene            complement(919..2055)
                     /gene="purM"
                     /locus_tag="VFX46_08680"
     CDS             complement(919..2055)
                     /gene="purM"
                     /locus_tag="VFX46_08680"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX5600698.1"
                     /translation="MSETDQPPRTPITYRDAGVDIDAGNALVEAIKPLARATRRPGGD
                     SELGGFGALFDLKGAGFTDPVLVASTDGVGTKLKIAIETGRHDTIGVDLVAMCVNDLL
                     AQGAEPLFFLDYFACGKLDVDTARTVIAGIAEGCRQAGCALIGGETAEMPGMYADQDY
                     DLAGFSVGAVERGSLLPRSDIAVGDVLIGLASSGPHSNGYSLIRRLVANEGLSWDAPA
                     SFAPDRSLAEALLEPTRIYVRPVLSAIRETGAIKAVAHITGGGLIENVPRVLPNGIAA
                     VVDLSSWTAPAVFGWLAQAGRLETAELLRTFNVGIGLVLIVSKSHADAVMTYLRAQGE
                     TPRIIGELAAGDGQRTQVKGSGAAGALRVVGELSFADPGPAGGA"
     gene            2234..>2782
                     /locus_tag="VFX46_08685"
     CDS             2234..>2782
                     /locus_tag="VFX46_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HEX5600699.1"
                     /translation="MSWLGVRHIRAAAAGVAAGLALSVVGFPASAEDEAYTVAAYPVD
                     AVASDAVTAKQKALAEGRQAAFRSLLKRLVPVTAYDRLDRVKHVKADELLEGVSVRDE
                     RTSATQYIASLDFSFRPEPIRDLLRSEGVPFVDEQAPAVTLIPVFLPAAAGQGAVPAE
                     LSAARGSRMWREVWAGLDLEHAL"
BASE COUNT          499 a          892 c          880 g          511 t
ORIGIN      
        1 acatgcgacg gcagcggtgc gctcatggtc gatgtcactc cgcgagtgtg gtcatccgaa
       61 cgcgaataca attaggattt ctacttcgac cgaatcggtt ctggcgacca attacttctc
      121 tatatcatcc gaccggggat ggcgtgggaa agtaaacgcc tggccggtga tcttcctgcc
      181 tcgtagcact agcgctccac acggtccaga ttatccgttt gacccgctac acaatggcgc
      241 gccgcggtcg gctgagcagt tagcctttgt attccaggga tggcgagaag agctgcgcag
      301 tctggttaac tgctccgtct acgacgaccc gtcggccttc gaggcggacc tggcccgctg
      361 cgacgaggcg aagagcgtgc ggatacaact tatgttctgc agagagtatt ctggcggcga
      421 gagtgtcggg cgtgtcgtcg tcggacacgg gaacggcagc ctgtgcaatg atggggcctg
      481 agtccatctc cggctccacg aaatggaccg tgcacccggc tatcttcaca cccgcggcaa
      541 gagcctgggc ctgcgcatca agccccttga aggcgggcag cagggaagga tggatgttga
      601 gcatccggcc tgaccacttc tcgacgaaac ccgccgtcat cagccgcatg aaccccgcgc
      661 agcagacgat ttcggccccg gatgcgacca gagcttcgtg cagtgcggcc tcgaagtcct
      721 cccggctagg atagctcttg tggtcgatga ctttcgtcgg aagtccctgg gaggcggccc
      781 acgctatccc tggagcttcg ggccggttcg aaatcaccaa aatgatttca gcgggatagc
      841 ttggatcccg cgcggcctcg acgagcgaca tcatgttcga gccgcgaccg gagatcagga
      901 tagccacgcg cttcttgctc atgctccacc cgccgggccg ggatctgcga acgatagctc
      961 gccgacaacg cgcagcgccc ccgccgcgcc gctgccctta acctgggttc tctgcccgtc
     1021 acccgcagcc agctctccga tgatacgtgg tgtttcgcct tgggctcgca ggtacgtcat
     1081 gacggcgtcc gcgtgggact tcgagacgat caagacgagg ccaatcccaa cgttgaaagt
     1141 tcgcagaagc tcggccgttt cgaggcgacc cgcctgggcc agccatccga agacggcagg
     1201 ggctgtccag ctcgacaggt ccaccactgc agcgatcccg tttggtagca cacgcggcac
     1261 attctcgatg agaccgccgc cggtgatgtg cgcaacggcc ttgatggctc ctgtctcgcg
     1321 gatggcggat agtactggcc ggacgtagat ccgcgtcggc tcgagcaagg cctctgctaa
     1381 cgaccgatcc ggcgcgaacg atgcgggagc atcccacgaa agcccctcat tcgcgacaag
     1441 gcgccggatc aaggagtatc cgttcgagtg ggggccggac gaggcaaggc cgatgagtac
     1501 gtctccaacg gcgatatcgc tccggggcag aagcgagccc cgctcgaccg cgccgacgga
     1561 aaaaccggcc agatcatagt cctggtctgc gtacatcccg ggcatctcgg ccgtctcgcc
     1621 gccgatcaga gcgcaaccag cctggcggca cccttcggca atcccggcga tgaccgtccg
     1681 cgccgtgtcc acgtcgagct ttccgcaagc gaaatagtcg aggaaaaaca acggctcggc
     1741 gccctgtgcg agcagatcgt tgacgcacat ggccacgagg tcgacgccga tggtgtcatg
     1801 ccggccggtc tcgatggcga tcttgagctt cgtgccaacg ccgtccgtgc tggcaaccag
     1861 caccggatcg gtgaagccgg cccccttcag gtcgaagagc gctccgaagc cgccgagctc
     1921 cgagtcccca ccgggccgac gggtagctct ggcaagtggc ttgatggcct cgacgagcgc
     1981 gttgccggca tcgatgtcga cacccgcatc gcgatacgtg attggtgttc ggggaggctg
     2041 gtcggtttca gacatcgtga tccttcgcgc cacactccgg ctactcgcgc cacggcccat
     2101 tttcgccgct cagctcaggc attgaccgga ccccattttg gcctcattgc tccccgtgag
     2161 atggggcgtc gtagcagact tcgcgcgcgg cgggcaactt gcgcatttgt acatcgcatc
     2221 gaggaggggc ctcgtgagct ggctcggagt gcgtcacatt agggctgctg cagctggagt
     2281 cgcagccggt ctggcgctct cagtggttgg tttccccgca tcggccgagg atgaggccta
     2341 tacggttgcc gcgtaccccg tcgatgccgt cgccagcgat gccgtcactg caaagcaaaa
     2401 ggcgctcgcg gaaggccggc aggctgcatt ccgctcgctt ttgaaacggc tggtgccagt
     2461 aacggcctat gatcggctcg atcgcgtcaa gcacgttaag gccgacgagc tgcttgaggg
     2521 cgtttcagtg cgcgacgagc gcacctccgc aacccagtac atcgccagcc tcgacttctc
     2581 gttccggcca gagcccatcc gcgatttgct gcgaagcgaa ggcgtgccct tcgtcgatga
     2641 gcaggcgcca gccgttacgt tgatccccgt tttcctgccg gccgcggcgg ggcagggggc
     2701 cgtacccgca gagctctcag cagcgcgcgg cagccggatg tggcgcgagg tctgggcagg
     2761 gctcgacttg gagcatgcgc tg
//
LOCUS       DASUMG010000523         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_553502, whole genome shotgun sequence.
ACCESSION   DASUMG010000523 DASUMG010000000
VERSION     DASUMG010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553502"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFX46_08690"
     CDS             complement(<1..932)
                     /locus_tag="VFX46_08690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5600700.1"
                     /translation="MARQPEQLPPREAMDFDVVIVGAGPAGLAAAIRLKQLALVDGTD
                     VSVVVLEKGSEVGAHILSGAVIDPIGLDALIPDWRERGAPVTTPVTEDRFVYLGPAGE
                     LRLPNFLMPPSMNNHGNYIVSLGNLVRWLGEQAAELGVEVYPGFAATEILKDEQGRVI
                     GVATGDMGIGRDGQPKDSFTRGMELRGKYTLIAEGAHGSLAKQLIRDFKLREGREPQK
                     YGIGIKELWEVSPERHQPGLIQHTFGWPLDNRTGGGSFLYHYGENLVAVGFVVHLNYE
                     NPYLSPYDEFQRFKTHPSIRPHLEGGKRIGYGARA"
     gene            1074..1976
                     /locus_tag="VFX46_08695"
     CDS             1074..1976
                     /locus_tag="VFX46_08695"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX5600701.1"
                     /translation="MTHQPDPRLVALLAWYRDMGVTDALADDPLDWLALGDRADLGIV
                     HGSSPPAAGRDQGLSQEPQVPRVPQAVTPSAPLPAAHRSFAPMPTSEAEDSARAAASS
                     ATSLEELRAALERFEGCALRATAKNLCFYRGAATGRVMIIGEAPGREEDLEGKPFVGR
                     AGQLLDKMLAAIGLSEADVHITNIVYWRPPGNRTPTPQEALVCRPFLMRQIELVQPEF
                     LLLLGAAAAKHLLGGTEGILRIRGKWREVEVGGRLIPAMASLHPAYLLRSPAQKRLAW
                     RDLLALKVRLDQGDRPGAANGVSG"
     gene            2006..>2489
                     /gene="moaB"
                     /locus_tag="VFX46_08700"
     CDS             2006..>2489
                     /gene="moaB"
                     /locus_tag="VFX46_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX5600702.1"
                     /translation="MSRIDETRPFIPVSIAVLTVSDTRQISEDKSGATLVELIEKAGH
                     KVAERAIVRDDIAQIRARVLSWIADPAINVVITTGGTGFTGRDVTPDAVRPLFDKEID
                     GFSTVFHMISFQKVGTSTIQSRACAGLAQETLVFCLPGSPGACRDAWNGILQYQLDNR
                     H"
BASE COUNT          442 a          779 c          775 g          493 t
ORIGIN      
        1 gcgcgggcgc catagccgat gcgctttccg ccttcgaggt ggggtcgaat ggatggatgg
       61 gtcttgaacc gctgaaattc gtcgtaaggg gagagataag ggttctcgta gttcaggtgt
      121 acgacaaacc cgacggcgac aagattctcc ccgtagtggt acaggaagga gccaccgccg
      181 gtgcgattgt cgagcggcca gccgaacgta tgctggatta gcccaggttg gtgccgttcg
      241 ggactaacct cccacaactc cttgatgccg atgccgtact tctgcggctc gcgaccttca
      301 cgcagcttga agtccctgat gagctgcttg gcgagcgatc cgtgcgcgcc ctcggcaatg
      361 agcgtgtatt tcccccgcag ctccatgcca cgggtgaagc tgtctttcgg ctgaccatcc
      421 cggccgatgc ccatgtcgcc tgttgcgacg ccgatgaccc tgccttgctc gtccttcagg
      481 atctccgttg ccgcaaaccc gggatagacc tctactccaa gctctgctgc ctgctcgcca
      541 agccaacgga ccaagttccc cagactgacg atgtaattgc cgtgattgtt catcgacggc
      601 ggcatcagga agtttggcag cctcagctcg cccgcaggac cgagatagac aaatcggtcc
      661 tcggtgactg gagtggttac gggggcgccc cgctccctcc aatcagggat gagagcatca
      721 agaccgatcg gatcgatgac tgcaccggag aggatgtgcg cgcccacttc ggagcccttc
      781 tccagcacga caaccgagac atcggtgccg tcaaccaacg cgagttgctt caggcgaata
      841 gccgccgcca agcccgcggg cccggcgccg acgatcacca catcaaaatc catcgcttct
      901 cgcgggggga gctgctccgg ctggcgtgcc attattcgaa atatctccga gtttgctgcg
      961 acgggcgcga ttacggccct tgtttgctca acgcgtcaag gctgcagcga catcgattcg
     1021 acctctgcga caagacccct cgcatggatt ctccttttgt ttagactgta gctatgacgc
     1081 atcaaccgga tcctcgcctc gttgcccttc ttgcgtggta tcgcgatatg ggcgtgacgg
     1141 acgcgttggc cgacgatccg ctcgactggc tggcgttggg ggatagggct gaccttggga
     1201 tcgtgcacgg ctcctcgcct cccgcggccg ggcgagatca aggtctttcg caggagccgc
     1261 aagttcctcg cgttccgcaa gccgtcactc cgtcggcgcc gttgccggcg gctcatcgta
     1321 gcttcgcgcc gatgccgacg tccgaggccg aggattcggc cagggcggcc gcttcctcag
     1381 cgacctcgct ggaggagcta cgggcggcat tggaacggtt cgaggggtgc gccctgcgtg
     1441 cgacggccaa gaacctgtgc ttctatcgtg gggcggccac aggacgcgtc atgatcatcg
     1501 gcgaggctcc cgggcgtgag gaggacctcg agggcaagcc gtttgtcggc cgcgccgggc
     1561 agttgcttga taagatgctg gcggccattg ggctcagcga ggcggacgtc cacatcacca
     1621 acatcgtcta ctggcgtcca cctggtaacc ggacaccgac gccgcaggag gcactggtct
     1681 gccggccgtt cttgatgcgg cagatcgagc ttgttcagcc ggagtttctg cttctgctcg
     1741 gtgcggcagc ggcgaaacat ctcttaggcg ggacggaggg tattttgcgc atcaggggta
     1801 agtggcgaga ggtggaagtt ggaggtcgcc tgatcccggc catggcgagc cttcatcccg
     1861 cctatctgct ccggagcccg gcccagaaac gtctcgcttg gcgcgacctg ctcgccctga
     1921 aggtgcgcct cgatcagggt gatcggcccg gtgcagctaa cggtgtttcc ggctagattg
     1981 gcgctcgaac tggagctttc ggaacatgtc acggattgac gagacgcgcc ccttcatacc
     2041 cgtgtccatc gcggtgctta ccgtctcgga cacgcgccag atcagcgagg acaagtcggg
     2101 cgccaccctg gtggagctga tcgagaaagc gggccacaaa gttgccgagc gggccatcgt
     2161 cagggatgac atcgctcaga tccgggcgcg cgtgctcagc tggatcgcag atccggctat
     2221 caacgtcgtc atcacgaccg gcggaaccgg cttcaccggc cgggacgtga cgcccgatgc
     2281 cgtaagaccg cttttcgaca aggagatcga cggtttctcg accgtctttc acatgatctc
     2341 gttccagaag gtcggaacct cgaccattca atcccgcgcc tgtgcgggat tggcccaaga
     2401 aacactcgtc ttctgtctcc cgggttctcc gggagcgtgc cgcgatgcct ggaatggcat
     2461 ccttcaatac cagctcgaca accggcacc
//
LOCUS       DASUMG010000524         3994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_312463, whole genome shotgun sequence.
ACCESSION   DASUMG010000524 DASUMG010000000
VERSION     DASUMG010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312463"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFX46_08705"
     CDS             complement(<1..478)
                     /locus_tag="VFX46_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX5600703.1"
                     /translation="MTSRPLLTIVLAAGKGTRMKSAIPKVLHCVGGLSLLGHVLTAIR
                     GLGEGRTAVVIGPEMDGVRAEVEKIAPKAEVFVQEPQLGTAHAVLAARRAIEQHEGDV
                     LVVFGDTPFITPSTLNGLLASLDASTPLAVLGFHAADPNGYGRLIMDGPGQVSAIRE"
     gene            818..1945
                     /locus_tag="VFX46_08710"
     CDS             818..1945
                     /locus_tag="VFX46_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600704.1"
                     /translation="MTVSDSKDDHVDTATPRPPRGSGLYTATWAVLAVTATGYIGALL
                     LEPEWAAPLITQSLRAEEPSPATPELVEQLTGEVNSLRETIANLERELTEVKAKAAAR
                     EVEIINQQKRAEIDPPQAPEEQEPVTAELQQEEAEMLGPVEAAQIETASLPARGDSGL
                     RATDSEPTHSDVSLGHTEAVEQPADTPTLAAVEEPQERQQPPIEILAVRRKGAEEEPG
                     AAQVELRETTPALTPPPPPAPVKKVAALSARPEVPSAGQKDQKAAPVATASLPRKPPA
                     IVFGPAIVTRASEAFAIRLDTGPSLDALRSRWRVLSSRHRNTLGDLEPRYLAVGTPGN
                     PSYELLAGPITSLEEASRICALLRDNMVPCSVGGQFDGEAL"
     gene            complement(1987..3423)
                     /locus_tag="VFX46_08715"
     CDS             complement(1987..3423)
                     /locus_tag="VFX46_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HEX5600705.1"
                     /translation="MVNVSFLQELLNTVAEQGRQLLPWVAKTGDTGKDIAELAKALVS
                     IRGEASGVAMARQILSLYRGLSDEQKHAFFRFLANELQPRPEVVQDAASAYLAEPSPA
                     ALSRLQAAVESPRQEFFRRLNLAPGATGEIVALRRDLLRMLPTDPSLHVVDADLRHLL
                     YSWFNRGFLVLRRIDWQTPAAILEKIIAYEAVHEIKGWDDLRRRLDPEDRRCFAFFHP
                     SLVDEPLIFVEVALTNDIADNITKLLEEAPKSGHANEPTTAIFYSISNCQDGLRGISF
                     GNFLIKQVVEELAREKPTLKTFVTLSPVPSFARWLATELEQDETGLIGPDLREALRSL
                     ANPRWLDDEEQVRMLEGPLTVLAAHYLVNVKGKDGRPLDPVARFHLGNGARLERINWL
                     ADTSPKGLRESHGIMVNYRYDLKEFERNHEAYANEGIVATSRAVRNLLRTPVKIRLRT
                     EPRQLPPPEKSHEDEVREPDEIIEPAGR"
     gene            complement(3689..>3994)
                     /locus_tag="VFX46_08720"
     CDS             complement(3689..>3994)
                     /locus_tag="VFX46_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600706.1"
                     /translation="CWVRAIRNASCKPIEVFPGKCAQFCRDECMAIAMVAASQHGACP
                     ALRACAWALIESSAIDEVVEGAECLGATLKSCQKVLSPASICAFAALCPLRPHGRPN"
BASE COUNT          777 a         1285 c         1266 g          666 t
ORIGIN      
        1 gctcccggat ggcgctcact tgcccgggac cgtccatgat caggcggccg tatccgttgg
       61 gatcggcggc atgaaatccg agcacggcga gaggcgtcga cgcgtcgagc gacgcgagca
      121 ggccattgag cgtcgacggc gtgatgaacg gcgtatcccc gaaaacgacc agcacgtcgc
      181 cctcatgctg ctcaatggcc cttcgcgccg cgagcaccgc gtgcgccgta ccgagctgcg
      241 gctcctggac gaagacttcc gccttcgggg caatcttctc cacctcggcg cgtaccccgt
      301 ccatctccgg accgatcacg acggctgttc ggccttcgcc aagtccgcgg atggcggtca
      361 agacgtgccc gagcagcgaa agccctccga cgcagtggag aaccttcgga atggccgact
      421 tcattcgcgt gcctttgccg gccgccagga cgatcgtgag gagtgggcga gaggtcatgg
      481 atccaccaac aggcattctc gggctcgccg gcgcccagaa atgggctcac ttccgcggcg
      541 cactcttctt ctagacacaa cttctagaca caacgctgac cggattcagc tggctcgatc
      601 cccaagcgag aggagccgga gataagggat atggccagga tgcggcaact tcgagggttt
      661 ccacaggcca agcacacagc gccctccccc atccgttgac cgggataacg gactcagagg
      721 acggtttggc gcaagcgaat cggtacttgg ccgcgcggac ggacctgcat cccgcgccca
      781 agctatggag agatcaaggg cggccatgcg agcgcaaatg accgtttcgg actccaagga
      841 cgatcacgtc gataccgcca cacctaggcc accccgtggc tctgggcttt atactgcaac
      901 gtgggccgtg ctggcggtaa ccgcgacggg ttacataggg gcactgttgc tggagcctga
      961 gtgggccgcc cctctcataa cacagtcgct acgagcagag gagccttccc ctgccacacc
     1021 tgagctggtc gagcaactga ccggcgaggt gaacagcctg cgagagacca tagccaactt
     1081 ggagcgggag ctgacggagg tcaaagccaa ggcagctgcg agggaagtgg aaatcatcaa
     1141 ccagcagaag cgcgctgaga tcgatccgcc ccaggcgccc gaggagcagg agccggttac
     1201 ggctgagctt cagcaggaag aggcggagat gctgggtccg gtggaggcag cgcagattga
     1261 aactgcctct ttgccggcaa ggggtgattc cggcctcaga gctaccgata gtgagccgac
     1321 acactcggac gtctcgcttg gtcacactga agcggtagag cagccggccg atacgcctac
     1381 gttggcggct gtggaggagc ctcaggaacg tcagcagcct ccaatcgaga tcctggccgt
     1441 aagaaggaag ggtgctgagg aggagcctgg agctgcgcag gtcgaattgc gggagacaac
     1501 gccggcgctc actccaccgc ccccgccagc gcccgttaag aaggtcgccg cactgagtgc
     1561 gagacccgag gttccatctg ccggccagaa ggatcagaaa gcagcaccgg tggcgacggc
     1621 cagcttgcca cgcaagccgc ccgcaatcgt gttcggtccg gctatcgtca cgcgtgcctc
     1681 tgaggcgttc gctatccgcc ttgacactgg gccgtcactt gacgcgcttc ggtcacggtg
     1741 gagggtgctc agcagccgcc accggaacac ccttggagat ctggagccgc gttatctcgc
     1801 agtcggcacg cccggtaatc cgtcctacga gcttctcgcc ggtccgatca catcgctgga
     1861 ggaagcgagc cgcatctgcg ctctgctccg ggacaacatg gtgccgtgca gcgtgggcgg
     1921 acaattcgac ggagaggccc tctgagcagc gtgcaccttc gggcggagcc cacgctgtta
     1981 gccgcctcaa cgtccggctg gctcgataat ttcgtccggc tcgcgaacct cgtcctcgtg
     2041 cgacttctcg ggaggtggaa gctgtcgggg ctccgtacgc agccgaattt tcacgggcgt
     2101 gcgcagcagg ttgcgtacgg cccgggacgt cgccacgatt ccttcgttgg cgtaagcctc
     2161 gtgattacgc tcgaactcct tcaggtcgta gcggtagttc accatgatgc cgtgtgactc
     2221 ccgcagaccc ttcggcgagg tatccgcgag ccaattgatg cgctcgagcc gcgcaccgtt
     2281 gccgaggtga aagcgggcga cggggtccag aggacggccg tcctttccct tcacgttcac
     2341 gagataatgt gcggccaaga ccgtcaaagg tccttcgagc atccgcacct gctcctcgtc
     2401 gtcgagccac ctgggattcg ccagtgacct gagagcctcc cgaaggtccg gcccgatcag
     2461 ccccgtttca tcctgctcca gctcagtggc aagccagcgc gcgaacgacg gaactggcga
     2521 cagggtcacg aacgtcttca gcgtcggctt ctcgcgtgcc agctcctcga cgacctgctt
     2581 gatcaggaag ttcccgaacg aaatgcccct cagcccgtcc tgacagttgg agatcgagta
     2641 gaaaatcgct gtcgtcggct cgttggcgtg gcccgatttc ggcgcttcct cgagcagctt
     2701 cgtgatgttg tccgcaatgt cgttcgtgag agcgacctcg acgaaaatca gcggctcgtc
     2761 gaccaacgac gggtggaaga aggcgaagca gcgccgatcc tccgggtcca accggcggcg
     2821 cagatcgtcc caacccttga tctcgtgcac cgcctcatag gcgatgatct tctccaggat
     2881 ggccgccggc gtctgccagt cgatgcggcg caagacgaga aagcccctgt tgaaccagga
     2941 gtagagcagg tgcctcagat ccgcgtcgac cacgtggagg gacggatcgg tcggcagcat
     3001 cctcaagaga tcgcgacgca gcgcgacgat ctcgccagtt gcgcccgggg cgaggttgag
     3061 ccgccggaag aactcctgcc gggggctttc cacggctgcc tgcaggcgtg acaaggccgc
     3121 gggcgagggc tccgcgagat aggcggaagc agcatcctgc acgacctccg gcctgggctg
     3181 cagctcattg gcgaggaacc ggaagaaggc gtgcttctgc tcgtccgaaa ggccgcgata
     3241 gagcgacagg atctggcggg ccatggccac gcctgaagcc tctccgcgga tcgaaaccag
     3301 cgcctttgcc agctcggcga tgtccttgcc agtatcaccc gtcttagcga cccaaggcag
     3361 aagctgccgc ccctgctccg cgacagtgtt gagcagctcc tgcagaaacg aaacgttcac
     3421 cacggcccaa cctcttgcat ggtctcccct gcttcgtggc catctcgccc gaaggcggat
     3481 gagcaaccat ggctatctag ctgacaaatg acaatgcttc gttggcaggg gacaaagacc
     3541 agattccgaa cgcctccgag cgtttccccg cagcgctacg aggataaccc ctggcgcgtc
     3601 aaccgacgcg acccaagatc gcgccgggaa gcatggcaaa gcaagagata gggatctggg
     3661 ccggtgggtc gaagcggtcc ccctcgggtc agttcggacg gccgtgcggc cgcaatggac
     3721 aaagcgcggc gaaagcgcag atcgaggccg gcgatagcac cttctggcag ctcttgagcg
     3781 tggcgcccag gcattcggcc ccttccacca cctcgtcaat ggcggagctt tcgatcagcg
     3841 cccaggcgca ggcccgcagt gcgggacagg ccccgtgttg gctggccgcc accatcgcaa
     3901 tggccatgca ctcgtcgcga cagaactgcg cgcacttgcc aggaaagacc tcgatcggct
     3961 tgcagctggc attgcggata gcgcggaccc agca
//
LOCUS       DASUMG010000525         6194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_954374, whole genome shotgun sequence.
ACCESSION   DASUMG010000525 DASUMG010000000
VERSION     DASUMG010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954374"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFX46_08725"
     CDS             complement(<1..533)
                     /locus_tag="VFX46_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600707.1"
                     /translation="MADDRVERGRTRVEGFQSGELDFQLMRSLGAGNYGGGTPGEVFA
                     TRAAVTGDDPYAWREALAAMGERILKAARQALERGHRVSARDHLLRASMYYRAAEYFA
                     DPWGSEAQTWGLASRDAFRAAAELIPDRIEPVEIPFEGKGLPGYFMAPANGADRGKTV
                     VVLTGFDGTAEELYFQVA"
     gene            919..1233
                     /gene="rplU"
                     /locus_tag="VFX46_08730"
     CDS             919..1233
                     /gene="rplU"
                     /locus_tag="VFX46_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX5600708.1"
                     /translation="MYAVIKTGGKQYRVAKDDVLVIEKIEGEAGSEVEFTEILMIGGN
                     GAPKIGTPTVAGAKVTAELVEQTRGPKLIAFKKRRRKNSRRKRGHRQDLTKVRITNII
                     GA"
     gene            1265..1537
                     /gene="rpmA"
                     /locus_tag="VFX46_08735"
     CDS             1265..1537
                     /gene="rpmA"
                     /locus_tag="VFX46_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX5600709.1"
                     /translation="MAHKKAGGSSRNGRDSHSKRLGVKKYGGEFVVPGNIIVRQRGTK
                     WHPGDNVGMGTDHTLFAKVAGIVEFRTKAKGRVFVSVRPSTLQAAE"
     gene            1678..2211
                     /locus_tag="VFX46_08740"
     CDS             1678..2211
                     /locus_tag="VFX46_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600710.1"
                     /translation="MTAAPLRTRRLLLRDLTPRDARRIAMLASDWDVARMTGRIPHPY
                     SEQLAYEWINDLAEGEFVQGIVHQNALIGICGYMPEGEGSAEIGYWIGKPWWGRGYAT
                     EAVGALIDYCFSVAGFERVTCGHFVDNPASARVIAKLGFRFIGTERVWCEARQTEVET
                     LKYERLRQDGEAKARVS"
     gene            2208..3251
                     /gene="obgE"
                     /locus_tag="VFX46_08745"
     CDS             2208..3251
                     /gene="obgE"
                     /locus_tag="VFX46_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX5600711.1"
                     /translation="MKFLDQAKIYVKAGDGGPGAVSFRREKFIEFGGPDGGDGGRGGD
                     VWVECVANLNTLIDYRYQQHFKAKNGQPGMGKNRSGAKGADCVLKVPPGTQIFAEDQE
                     TLIADLTEPGQRVLIARGGNGGFGNAHFKSPTNQAPRRANPGQPGEELTIWLRLKLIA
                     DAGLIGLPNAGKSTFLAKVSAAKPKIADYPFTTLHPNLGMVRVGDVDFVLADIPGLIE
                     GAHAGAGLGTRFLGHIERCRVLLHLVDATQEDVAGAYRVVRRELKAYDADLEKKKEIV
                     ALSKCDALDPDTIAERQEALRRVARKRPYLLSAVSGQGVQDVLYALSRVIDESREEER
                     REAEPVAARGWRP"
     gene            3257..4429
                     /gene="proB"
                     /locus_tag="VFX46_08750"
     CDS             3257..4429
                     /gene="proB"
                     /locus_tag="VFX46_08750"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEX5600712.1"
                     /translation="MVEAKAGVRAEESPQEAGLAAWARARRIVVKIGSSLLVDSNSGR
                     IKSTWLNSLADDIAELWHEQREIVLVSSGAIALGRQIMGLPKGPLHLEQSQAAAAVGQ
                     ITLAHSYQEVFRARGLTTAQVLLTLGDTEERRRYLNARSTIDTLLAHRAIPVVNENDT
                     VATAEIRYGDNDRLSARVASMISADCLVLLSDVDGLYTSPPGRDPNARRLDIVREITP
                     EIEAMAGDAGTELSRGGMKTKIEAGRIALAGGTDMVIASGKVLHPLRALRDGAPCTWF
                     LAPSDPVTARKRWIAGQLEPRGVVEIDAGAERALLAGKSLLPAGVTRVHGQFDRGDAV
                     IIRNSAGYEIGRGLIAYARADAERIIGRRSSEIPKILGYEGRTELIHRDDMALNRK"
     gene            4435..5727
                     /locus_tag="VFX46_08755"
     CDS             4435..5727
                     /locus_tag="VFX46_08755"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX5600713.1"
                     /translation="MNKLDRNVESAGTAELMRQMGIAARAAAREVALASSARKNEALV
                     AAARAIRKSAPIILEANAKDVEGARVNGATSAFIDRLTLNDARIEGIARSLEDIAKLD
                     DPVGATMAKWTRPNGLVIERVRVPLGVIGIIYESRPNVTADAGGLCLKSGNAAILRGG
                     SESLHSSRAIHACLLKGLHAAGLPEAAIQLVPTTDREAVGHMLKGLNGNIDVIVPRGG
                     KSLVARVQEEARVPVFAHLEGICHTYVDKDADLRMAREIVLNAKMRRTGICGATETLL
                     VDRSAPSDFLPVLVKALLDAGCEVRGDAATQAVDPRVTPATEEDWATEYLDAIISVKV
                     VDGVDEAIEHIARYGSQHTDAIVTGNAETAQRFLDRVDSAIVLHNASTQFADGGEFGF
                     GAEIGIATGKMHARGPVGVEQLTSFKYVVRGTGQVRPA"
     gene            5754..>6194
                     /locus_tag="VFX46_08760"
     CDS             5754..>6194
                     /locus_tag="VFX46_08760"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX5600714.1"
                     /translation="MRRSAPVSFGSVRVRTPLVAPGLRIGLLGGTFNPPHDGHVLISH
                     TALVRLQLDQLWWIVTPGNPLKETGDLPPLADRMAACRAMMPDARVRITGFEEQLGTP
                     YTAATLAFLKRRYPRVHFVWVMGADNLASFHRWQHWREIMCTMPI"
BASE COUNT         1165 a         1915 c         2043 g         1071 t
ORIGIN      
        1 gccacctgga aataaagctc ctcagcggtg ccgtcgaagc ccgtgaggac gacgaccgtc
       61 ttgccgcgat ccgccccgtt ggcaggcgcc atgaagtagc caggcaggcc ttttccttcg
      121 aaggggattt cgaccggctc gattcggtcc gggatcagct ctgcggcggc gcggaaggcg
      181 tcgcggctgg caaggcccca cgtctgcgcc tccgaccccc agggatcggc gaagtattcg
      241 gctgcccggt agtacatgga ggcgcggagg agatggtcgc gtgcactgac gcgatgtccg
      301 cgctccaagg cttggcgggc agccttcagg attcgttccc ccatcgctgc gagcgcctcg
      361 cgccacgcgt aagggtcatc tccggttact gccgcacgag tcgcaaaaac ctccccgggc
      421 gttccaccac cgtagtttcc tgcgcctagg gagcgcatga gctggaaatc gagttctccc
      481 gactggaagc cttcgacccg tgtcctacca cgctcgacgc ggtcatccgc catggttctc
      541 ctccttggtc gctcgtgcga cttgctcgcg aggcgcgttc gactgcttag gcaaccgaag
      601 gaggccaaat tggcttccct gcgcgtgctg gagctcgccg catccttcaa cctctagtaa
      661 gcggaggctc cgtttagagt tgcttgacgt ccggctgcgt cagcgcgagt gacgcggcgc
      721 tcccgaaaac attgacttcc agcgccgtcc cgacgatatt gcgcccgcac gcggtgggtt
      781 gggccatcgc gcaagaggcg ctgcccgagg ctcttcgccg gcatcctgcc ggtagaccgt
      841 ttctccagtg aggcggttca ccaaagtccg cgagatttgg ctcgcggcgc cacagggcgt
      901 ggagaagaag gacacgtcat gtacgcagtc atcaagaccg gcggcaagca ataccgtgtt
      961 gccaaggacg acgttctcgt catcgagaag atcgagggtg aggcggggtc cgaggtggag
     1021 ttcacggaaa tactgatgat cggaggcaac ggggcgccga agatcggaac gcctaccgtc
     1081 gcgggagcca aggtcacggc ggagctcgtg gagcagaccc gcggcccgaa gctcatcgcg
     1141 ttcaagaagc gccgtcgcaa gaactcccgg cgcaagcggg gccaccgcca ggatttgacg
     1201 aaagttcgga ttaccaacat catcggcgcc tgagggcagc cgacccgacc aagaggttac
     1261 ggatatggca cacaaaaagg caggcgggtc gtcgcgcaac gggcgcgact cgcattcgaa
     1321 acgcctcggt gtgaagaagt acggcggcga gttcgtcgta ccgggcaaca ttatcgtgcg
     1381 ccagcgcggc accaagtggc atcccggcga caacgttggg atgggcaccg accacacgct
     1441 cttcgcgaag gtcgcgggga tcgtggagtt ccgcaccaaa gccaagggac gcgtgttcgt
     1501 ctcggtccga ccgtccacgt tgcaggcggc ggagtaagcc gcacgaccag aggcgatggc
     1561 ttgttccaag ggagatggca agccatctcc ctttttcgtg tttccaagtt tctcttatct
     1621 ccgacagagc cggatccagg gatccggcaa gaaggggttc gagtgggagc cagtctcgtg
     1681 actgccgcgc cgttgagaac cagacgcctg cttttgcggg acctgacgcc gcgcgacgct
     1741 cgcagaatcg cgatgctggc gagcgattgg gacgtcgcgc gcatgacggg ccgcatcccg
     1801 cacccctact ccgagcagct cgcatacgag tggatcaatg atctcgccga aggtgagttc
     1861 gtgcagggca tcgttcacca gaatgccctg atcggaattt gcggatacat gcccgagggc
     1921 gaaggctcgg cggagatcgg ctactggatc ggcaaaccgt ggtggggacg cgggtatgcg
     1981 accgaggcgg tcggtgctct tatcgattac tgtttctccg tagccggctt cgagcgggtc
     2041 acttgcggcc acttcgttga caatcccgct tccgcgaggg tgatcgcgaa gctcggcttt
     2101 cggttcattg ggacggaacg tgtctggtgc gaagcccgcc agaccgaggt cgaaacgctg
     2161 aaatacgagc ggctccgcca ggatggcgag gctaaggcaa gggtgtcatg aagttcctcg
     2221 accaggccaa gatctacgta aaggccggtg acggaggacc gggtgcagtc agcttccgcc
     2281 gcgagaagtt catcgagttc ggcggacccg atggtggcga cggcggcagg ggcggcgacg
     2341 tttgggtcga atgcgtcgcc aacctcaata cgctgatcga ctatcgctat cagcagcact
     2401 tcaaagcgaa gaatggccag cccggcatgg gcaagaaccg ctcaggcgcc aaaggggcgg
     2461 attgcgttct caaggtgcct ccggggacgc aaatcttcgc ggaagatcag gagacgttga
     2521 ttgccgatct gacggaaccg ggccagcggg tcctgattgc ccgtggcggc aatggcggct
     2581 tcggcaatgc ccatttcaaa tcaccgacga accaagcgcc gcggcgcgcc aatcccggcc
     2641 agcccggcga ggagctgacc atctggctgc gcttgaagct gattgccgac gcgggcctga
     2701 tcggtctgcc gaatgcgggc aaatccacct ttttggccaa ggtcagcgcc gccaaaccga
     2761 aaatcgccga ctatccattt acgacgctgc accccaatct cggcatggtt cgcgtgggtg
     2821 acgtcgactt cgtgctggct gacatcccgg ggctcatcga aggggcgcac gccggcgcag
     2881 ggctcggcac gcgcttcctc gggcatatcg agcgttgccg cgtgctattg catctggtcg
     2941 atgctacaca ggaagacgtc gccggcgctt accgggtcgt ccgccgcgag ctcaaagctt
     3001 acgatgcgga cctcgagaaa aagaaggaga tcgtcgcgct ctcgaagtgc gacgcactgg
     3061 atcctgatac catcgccgag cggcaagagg cgctgaggcg ggtggcgcgc aagaggccct
     3121 atcttttgtc cgccgtgtcc ggacaagggg ttcaggatgt tctttacgcc ctctcgcgtg
     3181 tcatcgacga atcccgtgag gaggagcggc gcgaggcgga gcctgtggcg gcgagaggct
     3241 ggcggccatg agcggaatgg tggaagcgaa agcgggcgtt cgcgccgagg agtcgccgca
     3301 agaggctggg cttgctgcct gggccagagc gcggcggatc gtggtcaaga tcggctcctc
     3361 gctgctcgtg gacagcaact ccggccgcat caaatccacc tggctgaact cgctggccga
     3421 tgacattgcc gagctttggc acgagcagcg ggaaatcgtg ctcgtctcct cgggcgcgat
     3481 cgcgctcggc cggcagatca tgggcctgcc gaaaggcccg ctgcacctcg agcaatcgca
     3541 agcggcagcc gctgtgggcc agatcaccct cgcgcattct tatcaggagg tcttccgggc
     3601 gcgcggactg accacagcgc aggtgctact gaccctcggc gacacagagg aacgccgccg
     3661 ctatctcaat gcgcggagca cgatcgacac gctcctcgcc caccgtgcga tcccggtcgt
     3721 caacgagaac gacacagttg caacggcgga gatccgctac ggtgacaatg atcgcctgtc
     3781 cgcacgggtg gcgagcatga tcagcgccga ctgcctcgtg cttctgtccg atgtggacgg
     3841 cctctacacg agcccacccg gccgcgatcc caatgcccgg cgtctcgata tcgtccgcga
     3901 gatcacgccc gagatcgaag ccatggccgg cgatgcgggc accgagctgt cgcgcggcgg
     3961 catgaaaacc aagatcgagg cggggcgcat cgccctggcc ggcggcaccg acatggtgat
     4021 cgcctcgggc aaggtgctgc acccgttacg ggcgctccgc gacggcgcgc cttgcacctg
     4081 gttccttgcg ccctccgatc cggtaacggc tcggaaacgc tggatcgcgg gccaactcga
     4141 gcctcgagga gttgtcgaga tcgacgccgg cgcggagcgg gcgctccttg cgggcaagag
     4201 cctgctgccg gcaggagtca cacgcgtcca cggccagttc gatcgcggtg atgcagtgat
     4261 catccgcaac agtgcagggt atgaaatcgg ccgcggactg atcgcctatg cgcgcgccga
     4321 tgcagagcgt atcatcggcc gtcgcagcag tgaaatcccg aagatcctgg gatatgaggg
     4381 ccgcaccgag ctcatccacc gcgatgacat ggcattgaac cggaagtagg cgcgatgaac
     4441 aaactggacc gcaacgtcga aagcgccgga acggcggagc tgatgcgcca aatgggcatt
     4501 gcggcacgtg ccgcagctcg tgaggtggcg ctcgcctctt cggcgcggaa gaacgaagct
     4561 cttgttgccg cggccagggc cattcggaag tctgcgccca tcattctgga agccaatgcc
     4621 aaggatgtcg aaggtgcgcg ggtaaacggc gcgacgagtg ccttcatcga tcggctgacg
     4681 ctcaatgacg cgcgcatcga aggcatcgcg cgaagccttg aggacattgc gaagctcgat
     4741 gacccggtgg gcgcgacgat ggcgaagtgg acgcgtccca atgggcttgt catcgagcgg
     4801 gtgcgcgtgc cgctgggcgt catcggcatc atctatgaaa gccggcccaa tgtgacggct
     4861 gacgccggcg ggctttgcct taagagcggc aacgcagcga ttttgcgcgg tggctccgag
     4921 agcctgcatt caagccgcgc cattcacgcc tgtctcttga agggcctgca cgcagccggc
     4981 ttgcctgaag ccgcgatcca gctcgttccg acgacggatc gcgaagccgt tggccatatg
     5041 ctgaaagggc ttaacggcaa tatcgacgtc atcgtgcctc gcggtggaaa gagtttggtt
     5101 gcgcgggtgc aggaggaagc ccgcgtgccc gtgtttgcgc acctcgaagg catttgccac
     5161 acgtatgtgg ataaggatgc cgatctccgt atggcgcgcg aaatcgtgct caacgccaaa
     5221 atgcgccgca ccggcatttg cggagcgacg gagacgctgc tcgtcgatcg ctccgcgcca
     5281 tccgatttcc tgccggtgct ggttaaggcg ctcctcgatg ctggctgtga ggtgcgcggc
     5341 gacgcggcaa cgcaagcggt cgacccgcgg gtcacgccag caaccgagga ggactgggcg
     5401 accgaatatc tggacgccat catttcggtg aaagttgtcg atggcgtcga cgaggccatt
     5461 gagcacatcg ctcgctatgg ctctcagcac acggacgcca tcgtgaccgg caacgccgag
     5521 acagcgcagc gtttccttga tcgcgtcgac tcggccatcg tgctgcacaa cgcctcaacg
     5581 cagtttgcgg acggcggcga gttcggcttc ggcgccgaga tcggcattgc tactggcaag
     5641 atgcacgcac gcggaccggt gggcgtcgag cagctcacca gcttcaagta tgtcgtgcgc
     5701 ggcaccggcc aagtgaggcc ggcatgagtc gtgccggcgc gaggccctga atcatgcgca
     5761 ggagcgcgcc ggtttcgttc ggctcggtca gggtcaggac gccgctcgtc gctccaggct
     5821 tgcgcatcgg attgttggga ggaacgttca acccgcccca cgatggccac gtgctcatca
     5881 gccacacggc cttagttcgg ctccagctcg accagctgtg gtggatcgtg acacccggca
     5941 atccactgaa ggagaccggc gatctgccgc cgctcgccga tcgcatggcc gcctgccgtg
     6001 caatgatgcc cgatgctcga gtgaggatca ccggcttcga ggagcagcta ggcacgccct
     6061 acaccgccgc aacgctggct tttctgaagc ggcgctatcc tcgcgtccac ttcgtctggg
     6121 tcatgggcgc ggacaacctg gcaagcttcc atcggtggca acactggcgc gagatcatgt
     6181 gcacgatgcc catc
//
LOCUS       DASUMG010000526         4657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_632763, whole genome shotgun sequence.
ACCESSION   DASUMG010000526 DASUMG010000000
VERSION     DASUMG010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632763"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VFX46_08765"
     CDS             complement(<1..1329)
                     /locus_tag="VFX46_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600715.1"
                     /translation="MTLLRTVIWFAALPLSLAAGGAAGVSVTEGQAASTATPWQLAQA
                     PRVREAEDEGEERRRRGGREESQRGEKRGDRPERSQSRKDDGPQRSGRQQEPRPAAKP
                     AADKDRERTDKARSPARQATPAEEKAPRIEARPPIRPELLKRETLEKAREAVKAKEKE
                     AADQKKSDPRPGRREASGDRRDDGRDGRDRSSRDERKDARPAQQRDSQPSGDRRDDGR
                     DGRDRGARDDRRVGQPRRIEDIQRLRRQRVEGGRTIIEEPDKRVIVREKGRTIIRHDE
                     EERFRRRFRDARTERLRDGGRRTIFIGPGGVEIITEYDPHDRLLRRYRRHRDGRIVVL
                     IDNRRHHRPSLGGLIIGALLDLPPPVVRIPREKYIVEYEDASFEDIYEALSAPPLEEL
                     ERDYSLDEIRYNYALRARMRRIDLNTINFDLGSWEVTPDQYPKLERIARAI"
     gene            1618..2019
                     /locus_tag="VFX46_08770"
     CDS             1618..2019
                     /locus_tag="VFX46_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX5600716.1"
                     /translation="MSDSGQDHGSGRGEISREDREALKARAAGIGSRLEQVKARRAPQ
                     LDSAARGAALGQAFKIAIELVVGVLFGGLVGWALDRYFGSTPWLLVLFLVLGFAAGLM
                     NVVRSARRMQAGAEPLQRAAPSVGSNDEDED"
     gene            2071..2784
                     /locus_tag="VFX46_08775"
     CDS             2071..2784
                     /locus_tag="VFX46_08775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX5600717.1"
                     /translation="MHQFEINRLAEIEIFGIDASFTNSSLFMVLGVGLITGFFALAMN
                     GRSLVPTRMQSVAELCYEFVANMVRENVGQAGMKYFPFVFTLFTFILVMNLLGMLPYS
                     FTVTSHIIVTFALAALVFVVVTAIGFARHGVGYLKLFVPEGVPMWLMPLIVPIELISY
                     LIRPVSLSVRLFANMMAGHTMLKVFAGFVVSLGIFGFAPLLFTIAFTGLEFVVAFLQA
                     FIFAVLTCIYLNDAVNMHH"
     gene            2849..3073
                     /locus_tag="VFX46_08780"
     CDS             2849..3073
                     /locus_tag="VFX46_08780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX5600718.1"
                     /translation="MDPTAAKFIGAGLACFALIGAGIGIGNIFGNYLSGALRNPSAAP
                     GQFTNLLIGFALAEATGLFGLLIALIILFG"
     gene            3128..3676
                     /locus_tag="VFX46_08785"
     CDS             3128..3676
                     /locus_tag="VFX46_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HEX5600719.1"
                     /translation="MLASLVVLATAAAEATEQSGGLPQLHTPDFAPQLIWLAITFGAL
                     YFILSRWSLPRIAEVIEERQNRIQRDLDEAERLKSETEKALAAYEQELAAARSRASSI
                     AREMREKLAAEVEEERARVEAQIQAKLADAEKRIAEMKSAALGQINQIATETAGLIVE
                     KLIGAEVSAEEARRALEPVPGE"
     gene            3682..4173
                     /locus_tag="VFX46_08790"
     CDS             3682..4173
                     /locus_tag="VFX46_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX5600720.1"
                     /translation="MSIFATAEFWVAVAFFAFIGLLVYYNVPSMIAKALDSRADAIRQ
                     ELDEARRLREEAQQLLNDYLRKRREAEEEARAIIEQAKLEAEALAAETRRNLQESLER
                     QTKLAEEKIARAEAEALNEVRAAAVDSAIAAAERIIKSKVTPEVNARLVDQSMSELKR
                     KLN"
     gene            4450..>4657
                     /locus_tag="VFX46_08795"
     CDS             4450..>4657
                     /locus_tag="VFX46_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600721.1"
                     /translation="MRSTTIAAACLTLALGAGVAFGQTSQPGGGPSTGPASGISPNRA
                     TGISPEARGPSVLRGLPANPGLSGE"
BASE COUNT          771 a         1479 c         1438 g          969 t
ORIGIN      
        1 gatggcccgc gcaatacgct cgagcttcgg gtactggtcc ggagtgacct cccaggagcc
       61 caagtcgaag ttaatggtat tgagatcaat ccgcctcatc cgcgctcgga gcgcgtaatt
      121 gtagcggatc tcatcgagcg aatagtcgcg ctcgagctcc tcgaggggtg gcgcggagag
      181 cgcctcgtaa atgtcctcga aggaagcatc ctcgtactcg acaatgtatt tttcgcgcgg
      241 gatgcgcacg accggcggcg gcagatcgag cagcgcgccg atgatgaggc cgcccaggct
      301 cggccggtga tggcgccggt tgtctatgag tacgacgatg cgaccgtcac gatgccgccg
      361 atagcggcgc agcagccggt cgtgtgggtc gtactccgtg atgatttcaa cgccgccagg
      421 tccgatgaag atggtccttc tgccgccatc acgaagccgc tcggtccgcg catcacggaa
      481 gcggcgacgg aaacgctcct cctcgtcgtg gcgaataatc gtgcggcctt tttcgcgcac
      541 gatcacgcgt ttgtctggct cctcgatgat cgtgcggcca ccctccacgc gctgacggcg
      601 cagccgctgg atatcctcga tgcggcgggg ctgaccgacc ctgcgatcgt cgcgtgcgcc
      661 gcgatctcgc ccgtctctgc cgtcatcgcg acggtcaccg ctcggctgcg aatcgcgctg
      721 ctgggcgggc ctagcgtcct tgcgctcatc gcgcgagctg cgatctcgcc catccctgcc
      781 gtcatcccga cgatcaccac tcgcctcgcg gcgaccaggg cgcggatcgc tcttcttctg
      841 gtcggccgct tctttctcct tcgcctttac cgcctcgcgc gccttttcaa gagtctccct
      901 tttgagaagc tcaggtcgga tcggtggccg cgcctcgata cgtggcgcct tctcctcggc
      961 gggcgtcgcc tgccttgcag gagaccgagc cttatccgtg cgctctctat ccttgtccgc
     1021 agctggctta gcggccggtc gcggctcctg ttgccttccg ctccgctgcg ggccgtcatc
     1081 ctttcgcgat tggctgcgct ccgggcggtc accccgcttc tcccctcgct ggctctcttc
     1141 gcggccaccg cgccttctac gctcctcccc ctcgtcctcg gcttcacgca cccgcggtgc
     1201 ttgagccaat tgccaggggg tcgcggtcga cgcggcttga ccttcggtca ctgaaacacc
     1261 ggcagcgcca ccggcggcca gggataaagg aagtgctgca aaccagataa cagtccgaag
     1321 caatgtcatt cttaagcgct ctcttgtctg aactttctca gcaatgtggc cccgcaaatt
     1381 gtgacggttg tttgttgcct aggtcagctt ctcccgcgct accgctccta ccggtctgat
     1441 gcttgctggc agcggtcacg ccgcctcccg ttttgcctgc gcggtttcgc catacctggc
     1501 gaccggtgct cggttatgtc gtttccttga cacccctagg ggcccccact aaggtgcgcg
     1561 ccgtcgatta tcccgccgtg catcggccca acctcaagag ttcgggcgag tgtcgccatg
     1621 tctgatagcg gccaggacca cggctcggga cgcggagaga tcagccggga ggatcgtgag
     1681 gcgctgaaag cgcgcgcggc cgggatcggt agccgcctcg agcaggtcaa agctagacgg
     1741 gcgcctcagc tcgacagtgc tgcaagaggg gctgctttag ggcaggcctt caagatcgcc
     1801 attgagcttg tggtcggggt tctgttcggc ggtctggtcg gctgggcgct cgaccggtat
     1861 ttcggctcaa cgccctggct attggtgctg tttttggtgc tcggcttcgc cgccggcttg
     1921 atgaatgtgg tgcggagcgc ccggcgcatg caggcgggtg cggagccgtt gcaacgggcg
     1981 gcgccctctg tgggatcgaa cgacgaggac gaggattagg tagggggcga gaaggtggcg
     2041 actggcgctg cagatggggc ccacggcccc atgcatcagt tcgaaatcaa tcggctggcg
     2101 gagatcgaga tcttcggcat cgatgcctcg ttcacgaact cgtccctgtt catggttctg
     2161 ggcgtcgggc tgattacggg gttcttcgcg ctcgccatga acggccgttc gctcgtgccg
     2221 acacggatgc agtccgtggc cgagctctgc tacgagttcg tcgccaatat ggtgcgcgag
     2281 aacgtcggcc aagcgggaat gaaatacttc cccttcgtct tcaccctatt cacgttcatc
     2341 ctggtgatga acctgctcgg gatgcttccg tactcgttta cggtgaccag ccacatcatc
     2401 gtgaccttcg ctctggcggc cctcgtcttc gtcgtggtga ctgctatcgg atttgcccgg
     2461 cacggggtcg gctatttgaa gctcttcgtg cccgaaggcg taccgatgtg gcttatgccg
     2521 ctcatcgtac cgatcgagct gatttcttac ctcattcgcc cagtcagcct ctcggtgcgt
     2581 ctcttcgcca acatgatggc cggccacaca atgctgaagg ttttcgccgg cttcgtcgtc
     2641 agcctcggga ttttcggatt tgcgccgctg ctgttcacga tcgcctttac gggactcgag
     2701 ttcgtggtcg cgttcctgca ggcgttcatc ttcgcggtgc tcacctgcat ctacctcaac
     2761 gacgcagtga acatgcacca ctgaatccat gccccattcc cggggtgagt tcatcttcaa
     2821 ctctgtgacc tctgaaggga gactgtaaat ggatccgacg gctgccaaat tcatcggtgc
     2881 gggcttggcc tgcttcgccc tcatcggtgc aggcatcggc atcggcaata tcttcggcaa
     2941 ctatctgtca ggcgcgctgc gcaacccgtc cgcagctccg ggccagttca cgaacctgct
     3001 gatcgggttt gctctcgccg aggcgaccgg cctgttcggc ctgcttatcg cgctcatcat
     3061 ccttttcggt tgacctggaa gtcgatttcg agattgctcg cgatcgacca tccgcgcgga
     3121 gactgccatg ttagctagcc ttgtcgtgct cgccacggca gcagcagagg cgacggagca
     3181 gagcggtgga ctccctcagc tccacacgcc ggattttgcg ccgcagctca tctggctcgc
     3241 gatcaccttc ggtgcgctct atttcattct gtcgcgctgg tcgttgcctc gcatcgccga
     3301 ggtgatcgag gagcgtcaga accgcattca gcgggatctg gacgaagcgg agcgcctgaa
     3361 gagcgagacg gagaaggcac tagcggctta cgagcaggag cttgccgccg cgcgtagccg
     3421 tgccagctcg atcgctcgtg agatgcggga gaagctggcc gccgaagtcg aggaggagcg
     3481 cgcacgggtc gaggctcaaa tccaggcgaa gctcgcggat gccgaaaagc gcattgcgga
     3541 gatgaaatct gctgcgcttg gccagatcaa ccagattgcc accgaaacgg cgggattgat
     3601 cgtggagaag cttatcggcg ccgaagtgag tgctgaggag gctcgccgcg cgctcgagcc
     3661 ggtgcccggg gagtagtcag catgtcgatc ttcgcaacgg ccgagttctg ggttgcagta
     3721 gccttcttcg cgttcatcgg cttactcgtc tactacaacg tgccgagcat gatcgctaag
     3781 gcgctcgaca gccgcgccga cgcaatccgt caagagctcg acgaggcgcg ccgtcttcgc
     3841 gaggaggccc agcagctcct caacgactac ctgcgcaagc gccgtgaggc cgaagaggaa
     3901 gcaagagcta tcatcgagca ggccaagctc gaagccgagg cgctggcagc ggagacccgt
     3961 cgaaatctgc aagagagcct cgagcgtcag accaagctgg ccgaagagaa aattgctcgg
     4021 gcggaagctg aagcgctcaa tgaggtgcgc gcagctgcgg tcgacagcgc cattgctgcc
     4081 gctgagcgca tcataaagag caaggtcacg cccgaggtga atgctcgcct tgtggatcaa
     4141 agcatgagcg agctgaagcg caagctcaac tgaggggccg gttttcggcc ctttttactt
     4201 tctaggtgac gcccgtaccc caaagtccta ttgcaagttc tctgcggttg aggaggcgac
     4261 tccggttccc ctcaccacct aggcacgcct cctcacggca gctttgagcc gccactttcg
     4321 gcccacccgc tcgctgttgc gccgaaatcc tggcggcgca atcgctgctg cctcgtccgg
     4381 gttgtcttaa caaggaacat cctcgcggcc gaagcgttgc ttgccggacg tcactcggaa
     4441 gaggtagcaa tgcgcagcac cacgattgcc gcagcgtgtc tcactttggc ccttggtgcc
     4501 ggcgtggctt tcggccagac gagccagcca ggcggcggac cttcgaccgg gccggcttcc
     4561 ggcataagcc ccaatcgagc caccggcatt agccccgagg cgagaggacc ttcggttctc
     4621 cgcggcttac cagccaatcc agggctttcg ggcgaag
//
LOCUS       DASUMG010000527         2104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1036369, whole genome shotgun sequence.
ACCESSION   DASUMG010000527 DASUMG010000000
VERSION     DASUMG010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036369"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1097
                     /locus_tag="VFX46_08800"
     CDS             129..1097
                     /locus_tag="VFX46_08800"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX5600722.1"
                     /translation="MYSLLDEKTRRAHKLRNSLHSVLLLLGLGVVLSLSAMLVWGPSG
                     VIWALLAVAILVFFAPRVPPEAVMRMYRATEVHPRAAGQVGRVLQILSERAGLQRLPR
                     LFVIPSSTLNAFATGSPDNASIAVTAGLLRTLNLRELAGVLAHEVSHIRNNDLWVMGL
                     ADAMSRFTQALSTLAVMLASFNIMGMLVGVTFVSWWAILVLYLAPLVSSLLQLGLSRA
                     REYDADLEGAMLTGDPQGLISALAKLERYTGRFWEDLMFPVPGRRVPQPSLLRSHPST
                     EDRIARLRELERKPTLPPIIIEEEPLVGPWPTVVVGPRFRWPGIWY"
     gene            1104..2084
                     /gene="meaB"
                     /locus_tag="VFX46_08805"
     CDS             1104..2084
                     /gene="meaB"
                     /locus_tag="VFX46_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEX5600723.1"
                     /translation="MSESLHDRRKEGTIDTASQARDLATRIRAQERRAVARALTEAER
                     QSPLAAELLSELKQHVGRALVIGFTGPPGAGKSTLVDAYVREVRRRGLTAGVIAVDPS
                     SPVSGGAILGDRVRMTAGLDDEGVFVRSLASRGHLGGLSPAAVRMIDILDAAGYDRIL
                     IETVGTGQSEIDIAQVTDVRIVISAPGLGDEIQAMKSGLLEIADILVVNKADREGANI
                     TFQQLKSALAMRSGVRTQTPVLKTTALTGDGVPALVDAVEEIGESRLRLGAQIRRRMR
                     ARYLIARAAAELVEAEIRQSEAQHLDELADALLEGKLSAAEAAGRLLSRR"
BASE COUNT          335 a          671 c          690 g          408 t
ORIGIN      
        1 tgagcgagat ctcctcggat cagcacggtg ccctcggggc ccgtgctttt ccgtctaccc
       61 gccacgcgta cttttgcgtt agaccgttaa ccggacggca gcccacctga aggcgacggt
      121 acaagccctt gtacagctta ctcgatgaga agacccggcg ggcgcacaag ctgcgcaaca
      181 gcttgcattc cgtgcttctg ctgctcggcc tcggcgtcgt gctgagcctg tccgccatgc
      241 tggtctgggg gccgtcgggc gtgatctggg ccttgctggc cgtggcaatc ctcgtcttct
      301 tcgcgccgcg agttcccccg gaggctgtga tgcgtatgta tcgcgccacc gaggtccacc
      361 cccgtgcggc tggccaggtc gggcgggttc tccagatcct ctcggagcgc gcgggcctcc
      421 agaggcttcc gcgcctcttc gtgatcccga gctccacgct gaatgctttc gcaacgggaa
      481 gccccgataa tgcaagcatc gcggtcacgg cagggcttct gcggacgctg aacttgcgcg
      541 agcttgcggg cgtgctggcg cacgaggtca gccacatccg caacaacgac ctctgggtga
      601 tgggactggc agatgccatg agccgcttca cgcaggcgct ctctacgttg gccgtgatgc
      661 tggcgagctt caacatcatg ggcatgctcg taggcgtcac gttcgtgtcc tggtgggcga
      721 tccttgttct ctatctggca ccgctcgtct caagcctgtt gcagctcggg ctgtcgcgcg
      781 ctcgcgagta cgatgcagac cttgagggcg ccatgctgac cggtgacccg caagggctca
      841 tttcggcgct ggccaagctc gagcgctaca ccggccgttt ctgggaggac ctgatgtttc
      901 cggttccggg gcgtcgagtg ccgcagccct ccttgctgcg ctcgcatccc agtaccgagg
      961 ataggatcgc ccgcctgcgg gagctcgagc gcaaaccgac cttgcccccg atcatcatcg
     1021 aggaagagcc gcttgtcggc ccgtggccga ccgtggttgt cggacctcga tttcgctggc
     1081 cggggatctg gtactgaagg gctatgtctg aaagcctgca cgaccgtcga aaagaaggca
     1141 caatcgatac agcgagccag gcgcgggacc tcgcgactag gatccgtgcc caggagcggc
     1201 gggctgttgc ccgcgctctg actgaggccg agcggcaatc tcctttggct gctgagctcc
     1261 tgagcgagtt gaaacagcat gtcggccggg cgctcgtcat cggcttcacc gggccgcctg
     1321 gagccggcaa gtcgacgctg gtcgacgctt acgtgcggga ggtgaggcgc aggggcctta
     1381 ccgctggcgt catcgcggtc gatccgtcca gccctgtatc aggcggcgcc atcctcggtg
     1441 accgcgtgcg gatgacggcg gggttggacg acgaaggcgt tttcgttcga tctttggcat
     1501 cacgtggcca cttgggcggc ctctcgccgg cggccgtgcg gatgatcgat atcttggatg
     1561 ccgccggcta tgaccgcatc ctcatcgaga cggtcggcac cgggcagtcg gagatcgata
     1621 tcgcccaggt gacggatgtc cgcatcgtca tttccgcccc gggtctgggc gatgaaatcc
     1681 aggccatgaa gtcagggctg ctcgagatcg cggacatcct tgtcgtcaac aaggccgatc
     1741 gggagggtgc caacatcacg tttcagcagc tcaagtccgc cctcgccatg cggtccgggg
     1801 tgcgcacgca aacgccggtc ttgaagacga cggccctcac gggagatggc gtgcccgctc
     1861 ttgtggacgc cgtagaggag atcggtgaga gccggttacg ccttggagcg caaatacgcc
     1921 gtcgaatgcg ggcaagatac ttgattgccc gggctgctgc tgagctcgtc gaagcggaaa
     1981 tccgccagtc tgaagcccag cacctggatg agctcgccga cgcgctgctc gaaggcaagc
     2041 tctcagctgc tgaagctgcg ggccgcctgc tctccaggcg ctgacgctcc accgtcttac
     2101 cgga
//
LOCUS       DASUMG010000528         3202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_633349, whole genome shotgun sequence.
ACCESSION   DASUMG010000528 DASUMG010000000
VERSION     DASUMG010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633349"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..513)
                     /locus_tag="VFX46_08810"
     CDS             complement(232..513)
                     /locus_tag="VFX46_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600724.1"
                     /translation="MSDKTVLAVPGRKLSVPRLFKRTLQVSIASLLTAVVALTFILAN
                     NMLLGGGGPARALTTWLAFIKRPDIQATIVLTAIVTVLFVYWQRDRERK"
     gene            complement(651..1565)
                     /locus_tag="VFX46_08815"
     CDS             complement(651..1565)
                     /locus_tag="VFX46_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600725.1"
                     /translation="MENEPQPWRDIFFTSYDGLRLYARHYPAPGSRLRPVLCLPGLTR
                     NSRDFHDLAMTLSTGETARDVYALDYRGRGRSQYDRDWRNYTIAVEMLDVEDLIVLLG
                     LEKPTIIGTSRGGLIAMALAASRPKAIGAVVLNDIGPVIERDGLTRIAGYVGSMPLPS
                     NWDDAAKLVAELNRRAFPAVPEEQWAEVARQWFNDEKGYPAPGYDPALAKAFSATDGP
                     IPPLWPQFTALTRVPLLVLRGEHSDILSKTTVDEMCARHPNCIALTVPGQGHAPLLKD
                     APTISAIARFLADVDAGRPIAHVVGRAA"
     gene            complement(1609..>3202)
                     /locus_tag="VFX46_08820"
     CDS             complement(1609..>3202)
                     /locus_tag="VFX46_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5600726.1"
                     /translation="KDLASRAWREFDIPAAYEQGFLERFGSLLTEADHKWRFDRLLID
                     APRWSSQRKERAAAAKRIIPLLSKSEQTKAEARLAVFLRQKSAAKLMAALPADTKADW
                     GLAFQKADLLRRQGKDEEAWTILLSAPTDREEAVNPDGWWALRQAGAYDALRAGNPKM
                     AFELVREAGPLSVNERNQQTFLAGWLALRHLKDPRTALAFFEANEKSADGPLSRSKAL
                     YWLGRTHEALGNRSKARELYKLAALYKDTFHAHLAREKLGKDAPRLTVTPPKAPTAEQ
                     IDRFNNLDAVRAAVIAKKAGINNVLVRAFISELQRYFETEAEVAMVAHLAEALGDTQM
                     ALRVGKAAVARGMNLLYYSYPIHAFPAYDPLRTPPEPALLLALARQESEFNSATVSGA
                     GARGILQVMPGTAKHICRNYRIKCNVKRLMSDPSYNAMLASAYVGDRMDDVSGSYILT
                     LTSYNAGPGRTRQWVRELGDPRDPSVDPIDWIYQIPIEETRDYVQKVLSNLQVYRARL
                     GNHNGSIGADLVRGRQKPTAAP"
BASE COUNT          585 a          975 c         1034 g          608 t
ORIGIN      
        1 ggaatgcctt ccgggcgaaa tcgtatttcg acagccatcc tcgaatagtg tgctggcgcg
       61 atatcagacc caggacggtg acgtattcgt tctgcggcgg gcaaattccg gcgtgaaggt
      121 ggagcttgtg cgcgaaggcg agcaatcaac cggaaccgcg ccataggcaa cgcccgacgc
      181 gcctatgact cgcacgggca gcaattgaaa ttgagtgttc ctcgcggcgg actacttccg
      241 ctcgcggtcg cgctgccaat aaacgaaaag caccgtcacg atcgccgtga gcacgatcgt
      301 ggcctgaatg tccggccgct tgatgaaggc tagccacgtc gtgagcgccc tggccggtcc
      361 gccgcctcca agcagcatgt tgtttgcaag gatgaacgtc agcgcgacca ccgctgtcag
      421 gagcgaagcg atcgagacct gcagggttcg cttgaacaag cgaggaactg acagcttcct
      481 tcccggcacg gcaagaacag tcttatcgga catgaccagc cccgttctct cgacgcctgc
      541 gaaatttgct cagcgtacga ccgctacgcc gcgatcctga cgccacagcg aatcactcgc
      601 agcgagtgta cccgattcgg ctggttgctc cgagttcggc tctcggagcc tcaggctgcg
      661 cggccaacaa cgtgcgcaat cgggcgaccg gcgtccacat cggccaggaa gcgagcgatt
      721 gcagagatcg tcggagcatc cttgagcaga ggggcgtgac cttggcccgg gacggtcagt
      781 gcaatgcagt ttggatggcg ggcgcacatt tcgtccaccg tggtcttgct caagatgtcc
      841 gagtgctcac cgcggaggac gagcagggga acgcgggtca gggcggtgaa ctgcggccac
      901 agcgggggta ttggcccatc cgtggccgag aacgctttcg ccagggcggg gtcatacccg
      961 ggcgccggat agcctttctc gtcattgaac cattgccgcg ccacctctgc ccattgctcc
     1021 tccggcacgg cagggaaggc ccggcggttg agctccgcaa ccaatttcgc ggcgtcatcc
     1081 caattcgagg ggaggggcat actccccacg tagcctgcga tccgcgtcag gccatcacgc
     1141 tcgatcacag gcccgatatc gttgaggacg acggctccta ttgctttcgg ccgcgatgcg
     1201 gccagcgcca tggcgatcag cccaccgcgc gaggtgccga tgatggtcgg tttctcgaga
     1261 cctagcagga cgatgagatc ttccacgtcg agcatctcga cggcgatggt gtaattgcgc
     1321 cagtcgcgat catattggga gcgtccccgg ccgcggtaat cgagggcgta gacatcgcgc
     1381 gccgtttcgc cggtcgagag agtcatcgcg agatcgtgga agtcgcgcga gttgcgcgta
     1441 agccccggca ggcacagcac aggtctcaaa cgcgagcccg gcgctggata gtggcgggca
     1501 taaagcctta gaccatcata cgaggtgaag aagatgtccc gccagggctg gggttcgttt
     1561 tccatgacgc gctgtctcgt gggggagctg atgtcgataa agaaaggcct acggtgcggc
     1621 agttggcttc tgccggccac ggaccaaatc cgcgccaatg gagccgttgt gatttcctag
     1681 ccgggcccga taaacctgca aattcgaaag caccttttgc acgtagtcgc gcgtttcctc
     1741 gattgggatc tggtaaatcc agtcgatcgg gtcgacgctg ggatcgcgag ggtcgccgag
     1801 ctcgcgcacc cactggcgcg tgcgcccggg gccggcattg tagctcgtca gagtcaagat
     1861 ataactgccc gacacgtcgt ccattcggtc gcccacatag gcgctcgcca gcatggcgtt
     1921 atacgatggg tcgctcatca ggcgtttgac attgcacttg atccggtagt tccggcagat
     1981 gtgcttggcc gtgccaggca tgacctgcag gatgccgcgc gccccggcgc ccgacacagt
     2041 ggcgctattg aactcgctct cctgacgggc gagggccaga agcagcgcgg gctcgggagg
     2101 cgtgcggagg gggtcatagg ccgggaaggc atgaatgggg taggagtagt agagaaggtt
     2161 catgccccgc gccacagcgg ctttccccac gcgcagcgcc atctgcgtgt cacccagtgc
     2221 ttcggcgaga tgagcaacca tcgccacctc tgcctcggtc tcgaagtagc gctgcagctc
     2281 cgagatgaag gcgcgcacca acacgttgtt gatcccggcc ttcttggcga tgaccgcagc
     2341 gcggaccgca tcgaggttgt tgaagcggtc gatctgctcg gcggtcggtg ccttaggcgg
     2401 agtgacggtg agcctcggtg cgtccttgcc tagcttctca cgggcaagat gggcgtggaa
     2461 cgtgtccttg tagagagccg cgagcttgta cagctcccgc gctttgctcc gattccccag
     2521 cgcctcatgg gtgcgtccga gccagtagag agccttcgac cggctcaagg ggccatcggc
     2581 acttttctcg ttcgcttcga agaaagccaa ggccgtgcgc gggtccttca ggtgccggag
     2641 agcgagccag ccggccagaa aggtttgctg gttgcgctca ttgacgctga gagggccggc
     2701 ttcgcggaca agctcgaagg ccattttggg attgccggca cgcagggcgt cgtaagcccc
     2761 cgcctgccgc aaggcccacc acccgtcagg gttgaccgcc tcttcgcgat cggtgggcgc
     2821 ggagaggagg atggtccacg cctcttcgtc cttgccctgc cggcgaagca ggtcggcttt
     2881 ctggaaagca agtccccagt cggctttggt atctgctggc agggccgcca tcagcttggc
     2941 tgccgatttc tggcgcagga acacagccag ccgcgcttcg gccttcgtct gctcggactt
     3001 gctcagcagc gggatgatgc gcttggcagc tgcggccctt tcctttcgtt ggcttgacca
     3061 gcgcggagcg tcgatgagca gccggtcaaa ccgccacttg tggtccgctt ccgttagaag
     3121 actgccaaat cgttcgagaa acccctgttc atatgctgcg ggaatatcga actcccgcca
     3181 ggcgcgtgac gcgagatctt tc
//
LOCUS       DASUMG010000529         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_473215, whole genome shotgun sequence.
ACCESSION   DASUMG010000529 DASUMG010000000
VERSION     DASUMG010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473215"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="VFX46_08825"
     CDS             <1..633
                     /locus_tag="VFX46_08825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX5600727.1"
                     /translation="KPVVAQFGTVAASAAYIAGLGTDYIVARGNTITGSIGVIMQWPE
                     ISGLLDKIGVKVNEVKSGPLKANPSPFQPMDETGRALAESMIAESHQWFLDLVRTRRQ
                     VATNSIPGLEQGRVFSGREALQHKLIDEIGGETEAIRWLEERGVEKGLNVVDWKERSG
                     TGWGLGGVAGLARYLGGDFVVGLTALLTRDRTLGAFSLDGLVSVWQPSEN"
     gene            670..960
                     /gene="ihfB"
                     /locus_tag="VFX46_08830"
     CDS             670..960
                     /gene="ihfB"
                     /locus_tag="VFX46_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEX5600728.1"
                     /translation="MIKSELIQRIAAANPHLYHRDVERIVNVIFDEIVNALARGDRVE
                     LRGFGAFTVKYRAPRIGRNPRTGAPVPVEEKYVPFFKTGKELRERINRPSSS"
     gene            999..1373
                     /locus_tag="VFX46_08835"
     CDS             999..1373
                     /locus_tag="VFX46_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600729.1"
                     /translation="MIGRILGLLFGFVAAVFLVTLAVANRHTVQLVLDPFNPLDPILA
                     VELPFYAYLFAMLIVGVILGGIATWFSQSRWRRLARARGQDAARWRAEAERLTRERDV
                     LVAQHKPASQSYEKRHLALASH"
     gene            1388..2359
                     /locus_tag="VFX46_08840"
     CDS             1388..2359
                     /locus_tag="VFX46_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX5600730.1"
                     /translation="MRFIDAAAVEKALAYPQLVDVLEEAFRTGAISPPRHHHTIKLEG
                     RPDATLLLMPAWTSSAPGEETAGRYIGIKQVTVFPDNSRLGKPAIYGAYLLLSTETGE
                     PLALIDAPTLTVWRTAAASALAARFLARPDATRMLMVGAGALAPYLVRAHASVRPIRE
                     VMLWNRTRSKAEALQRKLANSGLALSVVDDLEGAVRTADLISTATISSAPLICGAWLQ
                     PGCHVDCVGAFRPDMRETDDDVVRRARIWVDTFAGGLSEAGDVVIPLNAGVIRESDIQ
                     GDLFGLARGTAPRRNSADEITMFKSVGASIEDLAAAVAVYEKADAAA"
     gene            2496..>2863
                     /locus_tag="VFX46_08845"
     CDS             2496..>2863
                     /locus_tag="VFX46_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX5600731.1"
                     /translation="MAIKVKICGLRTEATVAAALASGADYVGLVFYPPSPRSVSPEDA
                     ANLAQLARGRAQIVALFVDPDETLLDEVIRKVEPDLIQLHGKETPERVATIRRKYGRP
                     VMKAISVESSADADRALAYR"
BASE COUNT          530 a          821 c          932 g          580 t
ORIGIN      
        1 aagccggtcg tcgcgcagtt cgggacggta gccgcgtcgg cggcctacat cgccgggctc
       61 gggacggatt acatcgttgc acggggcaac accatcacgg ggtcgatcgg tgtcatcatg
      121 cagtggccgg agatttccgg cctgctggac aagatcggag ttaaggtgaa tgaggtgaaa
      181 agcggcccct tgaaggccaa tccctcgcct ttccagccca tggacgagac gggccgtgcc
      241 cttgccgaga gcatgatcgc cgaaagtcat caatggtttc tcgatctggt tcgtacccgg
      301 cggcaagtcg caaccaacag cataccgggt cttgagcagg gacgggtttt ctccggacgc
      361 gaggcgctgc aacataagtt aatcgacgag atcggcggcg aaaccgaagc catacggtgg
      421 ctcgaggagc gcggggtcga gaagggcctc aatgtggtgg actggaagga gcgcagcgga
      481 acgggctggg gtcttggcgg agttgccggt ttggcgcgct atctgggtgg cgatttcgtg
      541 gtcgggttaa cagcgcttct cacgcgcgat cgaacgctgg gggccttcag ccttgacggg
      601 cttgtgtcgg tttggcaacc ttccgaaaac tgattataat acaagcactt tttgtcgtcg
      661 gagcgtgtcg tgatcaagtc tgagctcatc caaaggattg ctgcagcaaa cccccatctc
      721 tatcaccgcg acgtcgagcg gatcgtcaac gtcatcttcg atgagattgt caacgcgttg
      781 gcgcggggag accgtgtcga gctcagaggt ttcggcgcgt ttacggtgaa atacagagcc
      841 ccgcggatcg gccgtaatcc ccggactgga gctccggttc ctgtggagga gaagtacgtg
      901 ccgttcttca agacgggtaa ggagctacgc gagcgcatca accggccctc atcgagctga
      961 agctttcgat tgggcgtctg gccgggaagg aacggattgt gattggtcga attctggggc
     1021 tgctgttcgg tttcgtcgcc gccgtttttc tcgtgacgtt ggcggtggcc aatcgccata
     1081 cggtgcagct tgtgctcgat ccattcaatc cactcgaccc gatcctggcc gtagagctac
     1141 ccttctacgc ttaccttttc gcgatgctca tcgttggagt catattgggt ggcatcgcaa
     1201 cttggttcag tcagagccgc tggcgccgat tggcgcgtgc tcgcgggcaa gacgcagcgc
     1261 gttggcgggc ggaggccgag cgactgacgc gcgagcgtga tgttctggtc gctcagcata
     1321 agcctgcgtc acagtcttac gaaaagaggc acctcgcttt ggcgagccac tgaactggac
     1381 ttcgcccatg cgcttcatag acgcggctgc ggtcgagaag gcgctggcat atcctcagct
     1441 cgtcgacgtg ctcgaggagg cttttcgcac gggagcgatt tcgccgccac gacatcacca
     1501 cacgatcaag ctcgaaggcc gcccagacgc tacactgctt ctgatgccgg cttggacaag
     1561 ctcggcgcca ggggaggaga cggctggccg gtacatcggg atcaagcagg tcaccgtttt
     1621 tcctgacaat tcccggctcg gcaaacccgc catctacggc gcctatctcc tcctttctac
     1681 ggaaacgggc gagccgcttg cccttattga tgcgcccacg ctcaccgttt ggaggacggc
     1741 tgcggcctca gcgctggcgg cgcgctttct ggccaggccg gatgccaccc ggatgctgat
     1801 ggtgggcgcg ggtgcgctcg cgccgtatct ggtgcgagcg catgcttcag tgcgacccat
     1861 tcgcgaggtc atgctgtgga acaggacgcg gtccaaagca gaggccctgc aacgaaaact
     1921 cgcaaactcc gggcttgctc tctccgtcgt tgacgatctg gagggagccg tgcggactgc
     1981 cgatcttatc tcgactgcga ccatatcctc ggctcccttg atctgcggtg cttggcttca
     2041 gcccggctgc cacgtcgact gcgtcggcgc gtttcggccg gacatgcgcg agactgatga
     2101 cgatgtggtc cggagagcac gaatttgggt cgatacgttc gcgggtggac ttagcgaggc
     2161 tggcgacgtc gtcatacctc tgaacgcagg cgtgatccgc gaaagcgaca tccaagggga
     2221 tctgttcggt ttggcccgtg gaaccgcacc gcgccgcaac tcggccgatg agatcacgat
     2281 gttcaaatcc gtcggcgctt cgatcgagga cttggcggcg gcggtggccg tttatgaaaa
     2341 ggctgatgct gcagcctagc gggcacgcag agatgtctgc acggctgcgg ggatgggtcg
     2401 agttgcgttt cctctccgct cggtgcacat agcgggagac cccatcgggg ctgaggcgcg
     2461 actgctgaag gcgtgcgccc gggcaggcat aggaaatggc aatcaaggtt aagatctgcg
     2521 ggcttagaac tgaggccaca gtcgccgcgg cgctcgcgtc cggtgccgac tacgtgggcc
     2581 tcgtcttcta tccgccgagc ccgcgcagtg tttcgcccga ggacgcagcc aacctagcgc
     2641 aacttgcgcg tgggcgtgcc cagattgtcg ccttattcgt cgatccggac gaaacgctcc
     2701 tggatgaagt gatccggaag gtcgagccag acctcattca acttcatgga aaggaaacgc
     2761 ctgagcgcgt ggcaactatt cgaaggaaat acgggcggcc cgtgatgaag gccatcagtg
     2821 ttgaaagtag cgcagatgcc gatcgcgcgc tcgcctaccg gga
//
LOCUS       DASUMG010000530         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_634076, whole genome shotgun sequence.
ACCESSION   DASUMG010000530 DASUMG010000000
VERSION     DASUMG010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634076"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..1409
                     /locus_tag="VFX46_08850"
     CDS             438..1409
                     /locus_tag="VFX46_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HEX5600732.1"
                     /translation="MRTLLIAVALIAGPAAGSATAQVYPARPITMVVPFAAGGSSDTI
                     GRIIADGMRGPLGQSVIIENVGGASGNLGVGRVARAAPDGYTLILGSWPTHVLNAAIF
                     ALPYDPLNDFEPVSLVAAQPLLVIGKKTLPPKNLVELIAWLKASPYTATQATAGAGGA
                     SHVAGVFFQRATGTRFQFVPYRGSGPGMQDLLAGQIDMMIDLAASSTPQVRAGTVKAY
                     AVTARSRSAAAPDVPTVDEAGLPGFHVLSWHAIWAPKATPKSVIAKLNDAVNEALADP
                     AVQKRLTDVGQEIFPREQHTPEALRAYQSAEIEKRWPIIRDANIKAE"
     gene            1508..2581
                     /locus_tag="VFX46_08855"
     CDS             1508..2581
                     /locus_tag="VFX46_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600733.1"
                     /translation="MGTTWLLDRTRRRFLQKAGAAAALGPLIISDRTIAQTRTLYVNS
                     WGGSYTAAQDAAFFKPFTAATGIQIRTVTPVAYGKIKAQVQSRTYEFDMTSVNSMQWL
                     RASREGLAEPIDWTIVKKDALPPNAVVADGYGIAQNILGTALCYRSDKYRDGGPRSWA
                     DFWDVKRFPGNRSLCLSDPPRTLIFALLADGVPRERLYPLDIDRAFRKLDQIKPHVKV
                     WWREGTQSQQLIRDGEVDMMSIWNARASELKQQGVPVEVVWNGAVRSTSTWSVLKGAP
                     NRKLAWELIAYAPQAKPQAEFNTRLFYGPIHPDAYAFIPKDIAVQLPTYEDNQAISVR
                     EDDQWEADRIVQIEQRFTQWLAS"
     gene            2651..>3256
                     /locus_tag="VFX46_08860"
     CDS             2651..>3256
                     /locus_tag="VFX46_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HEX5600734.1"
                     /translation="MIIQTAPSGKPRLAVMMHEHTALAGQFARAFGNDRFEAVLPDEL
                     MFYVVSHHDEGWAEFDRDPVTDERSGLPYNLVETPAEHITVTSRRSPEFNERRHPYCG
                     LLSSMHSWGLYNGRYGLSNLVLIDRIPSQDRPLAERMLDGEIDRQKRLKAEITKDPQT
                     ATWLEEKHLFQNYKQLQFFDTLALYFNRVHPSGRTEQKFEHV"
BASE COUNT          634 a         1013 c         1018 g          591 t
ORIGIN      
        1 gcgtggccga gcgcgtgtcc gcgccggtcc cggtgcgttg gctattgcgt actggttggc
       61 ataggatttg attgtactcg cagtgatttt accatctcct cgcggggcct tggagaagat
      121 ctgtctggaa tacctcgatc ttgtcggctt gagccgctac ccgcactact atcctatcag
      181 ctttccggcg gcatgcagca gcgcgtcgcg ctggccgggc gtcgaggcgc gcatgcgggt
      241 cgcctgattt tcgctagccg acagccgaga cgggagctcg cggctgtcag cgcgctcggc
      301 cgcgtagcgc aatgcgtttg gaatgcttag gattgcacgc cgaacgtttc ataaggcgag
      361 caggaccgct tgtcgccggc tcgatacgat atggccgacc cgatcccgca acactctcaa
      421 cgatcacggt gctatcgatg cggaccctgt tgattgccgt cgcattgatt gccggtccgg
      481 cggccggcag cgcgacggcc caggtctacc cggcgcgccc catcacgatg gtcgtcccct
      541 tcgccgcggg cggctcgtcg gatacgatcg gtcgcatcat cgccgatggg atgcgagggc
      601 cgctcggtca gtccgtcatc atcgaaaacg tgggaggcgc gtcgggcaat ctcggcgtcg
      661 ggcgggtcgc gcgcgccgcg cccgatggct acacgctcat cctgggtagt tggccgacgc
      721 acgtcttgaa tgccgccatt ttcgccctgc catacgatcc cttgaacgat ttcgagccgg
      781 tgtcattggt cgcggcccaa ccgctgctcg tgatcggcaa gaagaccctc cccccgaaga
      841 acctggtgga attgattgcc tggctgaagg cgagcccgta cacggcgacg caggcgacag
      901 ccggcgcggg cggtgcgtcg catgtcgcgg gggtcttctt ccagagagcg accggcaccc
      961 gctttcagtt cgtgccctat cggggcagcg gccccgggat gcaagatttg ctggcgggac
     1021 agatcgacat gatgatcgat ttggctgcca gctcgacgcc gcaggtgcgt gccggtaccg
     1081 tcaaagctta tgccgtcacc gccaggagcc ggtcggcagc agcgcctgat gttccaacgg
     1141 tcgacgaggc cggactgcca ggcttccacg tcttgtcctg gcacgcgatc tgggcgccaa
     1201 aggcaacgcc gaagagcgtc atcgccaagc tcaatgacgc cgtcaacgag gccctagccg
     1261 atccagcggt gcaaaagcgg ctaacggacg tcgggcagga gattttcccg cgcgagcaac
     1321 acacgccgga ggcgctgagg gcttaccagt cggccgagat cgaaaaaagg tggccgatca
     1381 tccgggatgc caacatcaag gcggagtgac ggattgcggg tcatcaccgg cggcatggag
     1441 tctcgcgcag ctcgtgcgat catcgtcgcc aaccggacga cagggtggca acagcaggag
     1501 gcggcagatg gggacgacct ggcttttaga tcgaacacgc cgcaggttcc tgcagaaagc
     1561 cggcgcggca gcggctttag gtccgctcat catctccgac cgcacgatcg ctcagacgcg
     1621 aacgctctat gtgaattcct ggggaggaag ttatacggcc gctcaggatg ccgcgttctt
     1681 caagcccttc accgcggcga caggcatcca gatccgcacg gtaacaccgg tggcatacgg
     1741 caagatcaaa gcgcaggtgc agagccgcac ctacgagttc gacatgacgt cggtcaactc
     1801 catgcaatgg ctgcgggcga gccgcgaggg cctggcggag ccgatcgatt ggaccatcgt
     1861 caagaaggat gcgctgcctc cgaatgccgt cgtcgccgat gggtacggta tcgcgcaaaa
     1921 tattctggga acggcgctct gctaccgtag cgacaaatat cgcgacgggg gtccccgctc
     1981 ttgggcggac ttctgggacg tcaagcggtt tcccggcaac cgcagcttat gcctgagcga
     2041 tccgccgcga acgctgatct tcgcgctgct cgccgacggc gtgccacgcg agcggctcta
     2101 cccgctcgac atcgatcgcg ccttcaggaa gctcgaccag atcaaaccac acgtcaaagt
     2161 gtggtggcgt gaggggaccc agtcgcagca actcattcgc gatggcgagg tcgacatgat
     2221 gtcgatctgg aatgcgcgcg cgagcgagct caagcagcaa ggcgtgccgg tcgaggtggt
     2281 ttggaatgga gcagttcgca gcacaagcac ctggtctgtg ctcaagggag ctcccaaccg
     2341 caagctcgcg tgggaactga tcgcctacgc cccgcaggcc aaaccgcagg ccgagttcaa
     2401 cacacggctc ttttacggcc caattcatcc cgacgcctat gcgtttatcc ccaaggacat
     2461 tgccgtgcag ctgcctacct acgaggacaa tcaagccatt tccgtccgag aggacgatca
     2521 gtgggaggca gaccggattg ttcagatcga gcagcgcttc acgcaatggc tcgcgtcata
     2581 gcgggcgggc gaacgacgga cgtcctgcca gctctgcgtc taccctgacg aattttggga
     2641 gcccctgacg atgatcattc agaccgcacc gtccggcaaa cctcgcttgg ccgtcatgat
     2701 gcatgagcac accgcgctcg cgggccaatt cgcccgtgcc ttcggcaacg atcgtttcga
     2761 agcggttttg cccgatgagc tcatgtttta cgtggtctca caccacgacg agggatgggc
     2821 cgagttcgac cgcgatcccg tgaccgatga gcgcagcggc ctgccctaca atctggtcga
     2881 gacgccagcg gaacacatta cggtcacgag tcgaagatcc ccggaattca acgagcggcg
     2941 gcatccctat tgcgggctgc tgtcgagcat gcacagttgg ggcctctaca atgggcgcta
     3001 cggtctctcg aatctggtgc tgatcgatag aattccgtcg caggaccggc ctttggccga
     3061 gcggatgctc gacggtgaga tcgaccgcca gaagcggctc aaggccgaga tcacaaaaga
     3121 cccgcagacg gcgacgtggc tcgaggaaaa gcacctattc cagaattaca agcaacttca
     3181 attcttcgac accctcgcgc tttatttcaa ccgcgtgcat ccgagcgggc gcaccgagca
     3241 aaaattcgag cacgta
//
LOCUS       DASUMG010000531         5962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_795275, whole genome shotgun sequence.
ACCESSION   DASUMG010000531 DASUMG010000000
VERSION     DASUMG010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795275"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2487
                     /locus_tag="VFX46_08865"
     CDS             <1..2487
                     /locus_tag="VFX46_08865"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEX5600735.1"
                     /translation="IVLLNATMGYVQQARAEEALAALRRMTADEAAVVRDGTTRVIPA
                     AEIVPGDIILLEEGDRVPADGRLIESISLHTAEAALTGESLPVEKDVGPIDADCPIGD
                     RHNMVFAGTAVTQGHGRAVITATGMQTQMGRIAGMLGETPDEVTPLQVELDRVGKLLG
                     AVVVAIAIVIIATILLVEDVSGLAIFDVLILGVALAVAAVPEGLPAIVTAVLSIGVQR
                     MAKRYAIVRHLASVETLGSANVIASDKTGTLTRNEMTVRVVVTASGRVTLSGTGYAPA
                     GEVTQGNGEPIGEALKAELEKALTIADRANNAELNNSDGRWTVQGDPTEGALVVAARK
                     IGLSGDTLGARYPRIAEIPFSSERKMMSTVHADAGNAERLLVMTKGAPDVLLSRCTHE
                     LVGERTQPLTDARRAEIQAACNGLAEEALRTLAVAFRPLSKTGTEQRRFDEGVEGDLV
                     FVGLFGLIDPPRREAKDSVQRAKRAGIRTIMITGDHPKTAAVIAKELDISADGRAISG
                     AQLDGMSDEELDRAVREVSVFARVNPEHKLRIVNALQRQGLTVAVTGDGVNDAPALKT
                     ANIGVAMGISGTDVSREAADIVLADDNFATIVAAIEEGRTIFANIRKFLRYLLSSNIG
                     EVMTMFFGVLLADVIGLAVTSNGLVLPLVATQILWINLVTDGAPALALGLDPSDDDVM
                     SRPPRPSGEGVLTGEMWRGIIFVGVIMAVGTLLVLDASLPGGLIEGTGTLAYAQTMAF
                     TTLVMFQLFNVLNARSDERSAFHGLLTNPWLWGAITLSVALQALVIYVPPLQQAFSTT
                     ALSFTDWIYCAAVASSVLWLREFSKAFKQL"
     gene            2553..3380
                     /locus_tag="VFX46_08870"
     CDS             2553..3380
                     /locus_tag="VFX46_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX5600736.1"
                     /translation="MTFDFSSILWLVLAAMVLQPLLVGRWLAFQRAQAIRAMEKAHGT
                     RVITMIHRQERRSLFGFSVSRHIDLEDAQSIIAAIKETPPDRPIDLVIHTPGGLVLAA
                     MQIARAIEAHPAKVTVYVPVYAMSGGTLIALAADEIVLGEFSVLGPIDPQIAGLPAAS
                     IVKARDSKPPASTFDLTLVLADVAEKALAQVKRGAVELLTPRLDQVSAEKLADKLAGG
                     HWTHDYALTAEEARELGLPVKVGMPREVLRLMKLYPQPIQQSAVEFLPIDVPGKRRI"
     gene            3510..4028
                     /locus_tag="VFX46_08875"
     CDS             3510..4028
                     /locus_tag="VFX46_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX5600737.1"
                     /translation="MSSSSCWLQLSAFGSCNGGAIALVFGLSLVPVILAAGLSADYAR
                     ALKVRQLLLNATEAAAIAGARLPQSEIDRRESVAKAAFAANILALPFATIEPKIVSED
                     DEILVEASYSMPTAFTNIAGIESIDLKVRTRARSAYSDGPVCLLSHKLTGDDKLRLER
                     LRESNDEMCQYR"
     gene            4320..5822
                     /locus_tag="VFX46_08880"
     CDS             4320..5822
                     /locus_tag="VFX46_08880"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX5600738.1"
                     /translation="MSTKTFAELGLSPKVLAAVEAAGYTTPTPIQAAAIPVALTGRDV
                     LGIAQTGTGKTASYVLPMITRLETGRARARMPRSLILAPTRELAAQVAQSFEKYGVNH
                     KLTVALLIGGVSMEDQTRKLDRGVDVLIATPGRLLDHFQRGRVMLMGVEILVIDEADR
                     MLDMGFIPDIEKICKLLPPKRQTLFFSATMPPEIQRLVDNFLNNPARIEVAPPATTAR
                     AITQRFVYCRDADDRAKREQLRQLIRSQDVRNAIIFCNRKRDVAILYRSLLKHGFNAG
                     ALHGDMDQKNRMETLDKFRSGEINILAASDVAARGLDIPEVSHVFNFDVPWAADDYVH
                     RIGRTGRAGREGFAATLVTRDELKLVKQIEKVTGEQPIWLGEPPPEEDFAEATRSKRR
                     RRRGGKAEDRKASATAEQKPKQESPQRKKKSAPPAEAPSSPVAQAADGTATAPKKAET
                     RAQPVKTQSTPAGDLPKAKEPQQEAASVGFADKIPAFLRRPVRIAKAANE"
BASE COUNT         1147 a         1888 c         1852 g         1075 t
ORIGIN      
        1 atcgtgctcc tcaacgcaac gatgggctat gtgcagcagg cgcgcgccga agaggcgtta
       61 gcagccttac gccgcatgac tgccgacgag gccgctgtcg tccgagatgg gacgacccgg
      121 gtcatacccg cagcggagat cgttccgggt gacatcatcc tccttgaaga aggggaccgc
      181 gttcctgccg atggacgcct gatcgagtcc atttctttgc ataccgccga agctgcactg
      241 accggggaga gccttcccgt cgaaaaggac gtcgggccta tcgatgcaga ctgccccatc
      301 ggcgaccgcc acaacatggt gtttgccggt acagcggtga cgcaggggca cgggcgcgcc
      361 gtcatcaccg cgaccggcat gcagacgcag atggggcgca tcgcgggcat gctgggcgaa
      421 acgcccgacg aggtcacgcc gcttcaggtg gagctcgacc gggttggcaa gctgctcggc
      481 gccgtggtgg tcgcgatcgc catcgtgatc atcgccacca ttctcctggt tgaggatgtc
      541 agcggtctcg cgatttttga cgtgctgatc ctgggagtgg cgctggccgt cgcagccgtg
      601 cccgagggtc tgccagccat cgtcaccgcc gtgctttcca tcggcgtgca acgcatggcc
      661 aagcgttacg cgatcgtccg ccatttggcc tctgttgaga cattggggtc agcgaacgtc
      721 atcgcctctg ataagaccgg aacgttgacc cgcaacgaaa tgaccgtacg cgtcgtggtg
      781 acggccagcg gccgggtcac gctcagcggc accggatacg cacctgcggg agaagtcacc
      841 caaggcaatg gcgagcccat cggcgaggca ctgaaggccg agttggaaaa ggctctcacc
      901 atcgcggacc gcgccaataa cgcggagctg aacaacagcg atggccgctg gaccgtccag
      961 ggcgacccga cggaaggagc cctggtcgtg gctgcgcgga aaatcgggct tagcggcgat
     1021 acgctagggg cgcgctaccc gcgaattgcg gaaatcccat tttcctcaga gcgcaaaatg
     1081 atgagcacgg tccatgccga tgctggcaat gcggagcgcc tgctcgtcat gaccaagggg
     1141 gcaccagatg tccttctatc gcgctgtacg cacgagctcg tgggcgagcg cactcagcct
     1201 ctcacagatg cgcgtcgtgc cgaaattcag gcggcgtgca acgggctcgc cgaggaggca
     1261 ttgcggacct tggcggttgc gttccgacct ttgtccaaga ccggaacgga gcagcggcgc
     1321 ttcgatgagg gtgtcgaggg cgacctcgtc ttcgtgggct tattcgggct catcgatccg
     1381 ccgcgacgcg aagcaaaaga ctcggtccag cgggccaaga gagccggcat acgcaccata
     1441 atgatcacgg gcgaccatcc gaaaacggcg gccgtcattg ctaaagaact cgacatctct
     1501 gctgacggtc gcgccatatc gggcgcccag ctggatggca tgtcggatga ggagctcgat
     1561 cgggcagtgc gcgaagtctc ggtctttgcg cgcgtcaatc ccgagcacaa gctgcgaatc
     1621 gtcaacgcgc tccaacgtca gggcctcacg gtggcggtga cgggagacgg cgtcaacgac
     1681 gcgccagcgc tcaagacggc caatatcggc gtggcgatgg ggattagcgg aaccgacgtt
     1741 tcgcgcgagg ccgccgacat cgttctcgct gacgacaact tcgcaacgat cgtcgcggca
     1801 atcgaagagg gccgtacgat cttcgccaac atccgcaagt tcctccggta tctgctctcg
     1861 tcgaacatcg gcgaggtcat gacgatgttc ttcggcgtac tgctcgccga tgtcattggg
     1921 ctcgccgtga caagtaacgg actcgtcctc cctctcgtcg caacgcagat cctctggatc
     1981 aatctcgtga ccgacggtgc gcccgccctc gctctcggtc tcgacccgtc cgatgacgat
     2041 gtcatgagcc gaccaccgcg cccgtcgggt gaaggcgtgc tgactggaga aatgtggcgc
     2101 ggcatcattt tcgtcggcgt gatcatggcg gttggcacgc tgctcgtgct cgatgcatcc
     2161 ctccccggcg gcctgatcga ggggacgggc acgctggctt acgcgcagac gatggcgttc
     2221 acgacgctgg tgatgttcca gctgttcaac gtgctcaatg cgcgatcgga tgagaggagc
     2281 gccttccacg ggctgctgac aaacccttgg ctctggggag cgatcacact ctctgtcgcg
     2341 ctgcaggcgc tcgtgatcta tgtcccgccc ctgcagcagg ccttctcaac gacagccctg
     2401 agcttcactg attggattta ctgcgcagca gtggcaagct cagttctgtg gctgcgggag
     2461 ttcagtaaag ccttcaagca gctgtgaacg aagcgaccgg cggcggcgcg atggcccgcc
     2521 ggcgcgatgc tgcatgacgg agaacgcctt taatgacctt cgatttctca tcgatcctct
     2581 ggctggtgct cgcggcaatg gttcttcagc cgctgctcgt cggccggtgg ctggcttttc
     2641 agcgcgcgca agcgatccgc gcgatggaga aggcgcacgg aacgcgggtc atcacgatga
     2701 tccaccggca ggagcggcgc agcttattcg gcttctcggt atcccggcat atcgatctcg
     2761 aagacgccca gagcatcatc gcagccatca aggagacacc gccggaccgt cccatcgacc
     2821 ttgtcattca tacacccggc gggctggtgc ttgcggccat gcagatcgcc cgcgccatcg
     2881 aggcccatcc ggcgaaagtg accgtctacg tccctgtcta tgccatgtcg ggcggaactt
     2941 tgattgcgct cgccgcagac gagatcgtcc tcggcgagtt ctcggtcctc gggccgatcg
     3001 acccgcagat cgccggattg ccggcggcca gcatcgtcaa ggcgcgcgac tccaagcctc
     3061 ctgcctcgac tttcgatctg acgctcgtgt tggcagacgt cgccgagaag gcgctcgcgc
     3121 aagtcaagcg cggcgcggtg gagcttctga cgccgcgcct cgaccaggtt tcggcggaaa
     3181 agctggccga caagctggcc ggcgggcact ggacccacga ttacgcgctg acggctgaag
     3241 aggcccgaga gctcggcctg cccgtcaagg tcggcatgcc gcgggaggtg ctcaggctga
     3301 tgaagctcta tccgcagccc attcagcagt ccgcggtcga gttcctgccc atcgatgtcc
     3361 cgggcaaacg gcggatttga gcttggaaac cctttcttag cctaagcagt cctgcttcct
     3421 gttctttcga cggcctttgt ttcaaattcc ccaagcgccg gcgggagcga gccttctcct
     3481 ccgccggacg ccgctggggg gagtaagacg tgtcatcatc atcgtgctgg ctgcagttat
     3541 ccgcattcgg gagctgcaac gggggcgcta ttgctctggt cttcggtcta tcactcgttc
     3601 ccgtaatttt ggctgccggt ctatcggccg actacgcacg agcgctcaag gttcgccagc
     3661 tgcttctgaa cgcaacggag gccgcggcaa tcgccggagc gaggctgcca cagagcgaaa
     3721 tagacaggcg ggagagtgtc gcgaaggctg catttgctgc caacatcctc gcattgccgt
     3781 tcgcaacaat cgagccaaaa atcgtgtcag aggacgacga aattctcgtc gaagcgagct
     3841 actccatgcc gacggccttt accaatattg caggcatcga atcgatagat ctcaaagtga
     3901 ggacacgggc gcgctccgcc tatagcgacg gccctgtctg cctcctatct cataaactaa
     3961 caggagacga taaacttcgg ttggagcgcc tccgtgaatc gaacgacgag atgtgccagt
     4021 accggtgaaa ccacttcacg cggcacaact cgtggtaaag tgtggcagct gagtgcctcc
     4081 cttcgcgcct gcgactctga cacgcggttc gaaggcacag tttttcttgc gttcgacaac
     4141 catatatgtc agcaggggct gtgctgagcg caggggatgt attcatttag tcccttttcg
     4201 cacacgcttt ctttccattt gccacatttg gcgatcccga aattccccct gcgctcgatc
     4261 ggccgccatt cgtcggagaa gtgcggccca tatgaacgca gatgaggaac gaggaatttt
     4321 tgtcaacgaa aacattcgct gagcttggac tcagcccgaa agtcctggcc gccgtcgagg
     4381 cagccggcta caccacgcca actcccattc aggccgcagc tattcccgtc gccttaaccg
     4441 gtcgggatgt gctcgggatt gcccagacgg gcaccgggaa gacggcatcg tacgtgcttc
     4501 ccatgatcac gaggctggaa accggccggg cccgggcgag gatgccgcgg tccttgatcc
     4561 tggcgccgac acgcgagctg gccgcccagg tggcccaaag cttcgagaag tacggtgtca
     4621 accataagct caccgtggcg ctgctgatcg gcggcgtgtc gatggaggat cagacccgga
     4681 agctcgaccg cggcgtcgac gtgctgatcg ccactccagg gcgcctcctc gatcacttcc
     4741 agcgcggccg cgtcatgctc atgggggtcg agatcctggt catcgacgaa gccgaccgga
     4801 tgctcgacat ggggttcatc ccggacatcg aaaagatctg caagctcctg ccgccgaaac
     4861 ggcagacgct cttcttctcc gcgaccatgc cgcccgagat ccagcggctg gtcgacaact
     4921 tcctcaataa ccccgcacgc atcgaggtgg ctcctcccgc cacgacggcg agggccatca
     4981 cgcagcgctt cgtgtactgc cgtgacgccg atgaccgggc caagcgcgag cagctccgtc
     5041 agctcatccg cagccaggac gtgcggaacg ccatcatctt ctgcaaccgc aagcgcgacg
     5101 tcgccatcct ctacaggtcg ctgctgaagc acggcttcaa tgccggcgcg ctccatggcg
     5161 acatggacca gaagaaccgc atggagacgc tcgacaagtt ccggtcgggc gaaatcaaca
     5221 ttctggcggc gagcgacgtt gcggctcgtg gcctcgatat tcccgaggtg agccacgtct
     5281 tcaacttcga cgtcccatgg gctgccgatg actatgtcca ccgcatcggt cgcacaggcc
     5341 gtgccggccg agagggcttc gccgcgacgc tggttacgcg tgacgagctc aagctcgtga
     5401 agcagataga gaaggtcacg ggcgagcagc cgatctggct cggcgagcct ccgccggagg
     5461 aagactttgc ggaagcaacc cgcagcaagc ggcgcaggcg ccgtggcggt aaggccgagg
     5521 accggaaggc aagcgcgacc gccgagcaga agcccaagca ggaatctccc caacgcaaga
     5581 agaaatccgc accgcctgca gaggctccct cgtctcccgt cgcccaagct gcggatggga
     5641 ctgcaacggc tccgaaaaag gctgagacaa gggcgcagcc cgtgaagact caatccacac
     5701 cggctggcga cttgccgaag gcgaaggagc ctcagcaaga ggcggcaagc gtcggcttcg
     5761 cggacaaaat cccagcgttc ctgcgccgcc ccgtccggat cgcaaaggcg gccaacgagt
     5821 aagccttgga cgagcgagaa tatcaggcgg agcgcgtgcg cttcgccttc ttgcgttgcg
     5881 cccggcgagc tgcttccaac gcggcgcgcg cccaggtcag tagctcatca ggatcatcga
     5941 gcaattcgtc cggcgcccga ca
//
LOCUS       DASUMG010000532         3005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_234117, whole genome shotgun sequence.
ACCESSION   DASUMG010000532 DASUMG010000000
VERSION     DASUMG010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234117"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..705
                     /locus_tag="VFX46_08885"
     CDS             160..705
                     /locus_tag="VFX46_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600739.1"
                     /translation="MTLKLSTTKTFTLVAGILAGVALSAPAAEAGMRIYFGFPVGGFY
                     VGGGHHHYRAHKYHAWRYHDLRRARAIEAERARKAAIAAERAEQKRLAALAVAKRKAA
                     DARADAGAKEPEAAEITLAAIPLPVKKPDNPNASEAGTLTTAAITTAANSAPFGEEPK
                     KLECKRYFANVGLTITVPCTE"
     gene            complement(806..1420)
                     /locus_tag="VFX46_08890"
     CDS             complement(806..1420)
                     /locus_tag="VFX46_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX5600740.1"
                     /translation="MTVRRQPQKFTDNGYLEGQLLIAMPAMADKRFARSVIYMCAHSD
                     EGAMGLIINQRAPNLSFADLLERLQLLDEETEAALPPELMRMAIHVGGPVEPERGFVL
                     HSSDYFTPSSTLPISDEVCLTATIDILKALAAGEGPDRAILALGYAGWAPGQLESEIH
                     ANGWLSCQADLDLIFDLDVEEKYERALSKLGIDPIHLVNAAGHA"
     gene            1572..2429
                     /locus_tag="VFX46_08895"
     CDS             1572..2429
                     /locus_tag="VFX46_08895"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEX5600741.1"
                     /translation="MVLNGRMRITAAILAGSIALNVEAVASPLATPWVEAHGAKVRLV
                     AGSVASKDAEGPTVVAGLAIHLNPGWKTYWRSPGDAGGLPPHFNWSASVNLSEANVLY
                     PAPRRFKDSVGDSVGYSGAVVFPIEVRPQDPDKPVELRLTLSYGICRDICVPAEAQLA
                     LTLPANATVPASPEIANALAAVPRGPDERQPDDPELVTSKAHLDGDRPKLMLEARFPG
                     SAASGDMFVEAMDGAYVPLPTRVAEKGDTISFEVDLSEATPKELRGKTLRITMVSDAG
                     QSEAYWTID"
     gene            2535..>3005
                     /locus_tag="VFX46_08900"
     CDS             2535..>3005
                     /locus_tag="VFX46_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX5600742.1"
                     /translation="MTIKVGDRLPDATFTIMGPEGPKQVPTNEVFGGKKVALFAVPGA
                     YTPTCHKQHMPGFVANADEFKNKGIDMIACTSVNDIFVLTQWAKDTGAEGKITMLSDG
                     NADFVRKIGLDIDLSARGLGLRSKRYSMIVDDCVVKVLNVEDAPPQHDKSSAANL"
BASE COUNT          594 a          920 c          937 g          554 t
ORIGIN      
        1 atcagctcga tccgcagcag ccacccgctg cccgcagtgt gagaaaggtg cgggctgttc
       61 cgcgcgacac agcctgccat ccccgtccca gccatgatgt caggcattgg agcgcagcaa
      121 gcgacatctc agcgaaacgc ctgactatgg tggacggata tgaccctcaa gctttcaacc
      181 acaaagacct tcacgctcgt tgcgggcatc ctcgcgggcg tggcgctctc ggctccggct
      241 gccgaggcgg gcatgcggat ctacttcggc ttcccagtcg gcggcttcta tgtcggcggt
      301 ggccatcacc actatcgtgc ccacaagtat cacgcgtggc gatatcatga cctccgccgg
      361 gccagggcga tcgaagccga gcgtgctcgg aaggcggcga tcgcggcgga acgggcagag
      421 caaaagcgtt tagctgctct cgctgtggca aagcggaagg cagctgatgc gcgcgcagac
      481 gccggggcga aggagcctga agctgccgag atcacgttgg cagcgatccc attgccggtg
      541 aagaagcccg acaacccgaa cgcgagcgag gcggggacac tcacgactgc agcgattacg
      601 acggccgcga attcggcacc atttggcgag gagccgaaaa agctcgagtg caagcgctac
      661 ttcgccaatg tcgggctgac gatcacggtg ccttgcaccg aatgatgtca cctccgaaaa
      721 gcaagggcca gacccgccgg cagcaggtct ggcccttatg cgttccgttc gtgtccgccg
      781 caggctttga aagagcccgg gtagatcagg cgtgacccgc ggcgttcacg aggtgaatcg
      841 ggtcgatacc cagcttggac agcgcccgct catacttctc ctcgacatcg agatcgaaga
      901 tgagatcgag atccgcctgg cagctcagcc agccattggc gtgaatctcg ctctcgagct
      961 gtcctggcgc ccaaccggcg tatccgaggg caagaattgc tcgatccggt ccctcgcctg
     1021 ccgccagggc cttcaggatg tcgatcgtgg cggtgaggca aacctcatca ctgatcggca
     1081 aggtcgagga gggggtgaag taatccgagc tgtgcaggac gaaccctcgc tcgggctcca
     1141 cggggcctcc gacgtggatt gccatgcgca tcaactccgg cggaagggct gcctcggtct
     1201 cctcgtccag gagttgcaac ctttcaagca aatccgcaaa gctgaggttc ggggcgcgct
     1261 gattgatgat cagccccatc gccccctcgt ccgaatgggc gcacatgtag atgaccgagc
     1321 gcgcaaatct cttgtcggcc atcgcgggca tggcgatgag caactgccct tcgaggtatc
     1381 cgttgtccgt aaacttttgt ggctgccgtc gtactgtcat tccgggagtc tagcctcagc
     1441 ctccactacc tgtgaagcat tgaatttgct ggcacgccga aagctgcttc acatgtcaaa
     1501 ttgatcgtga cttgcccgac actcaacctt ggctacatag gcataagtcg ccacgaggca
     1561 aggggactag agtggtgctc aatggtcgta tgcgaatcac cgccgccatc ctggcgggct
     1621 ccatagcact caatgttgag gccgtggcca gcccgctcgc gacaccgtgg gtcgaggcgc
     1681 acggcgcgaa ggtccggttg gtcgcgggtt cggttgcgtc gaaggacgcc gaaggcccca
     1741 ccgttgtcgc cggcctcgcg atccatctga acccgggctg gaagacctat tggagatcgc
     1801 ccggagacgc cggcgggttg ccaccgcact tcaactggtc agcatcggtg aacctgtccg
     1861 aggcgaatgt cctttatccg gccccgcgtc ggttcaaaga ctcagtggga gattcggtcg
     1921 gctactcggg agccgtggtg tttccgatcg aggtgcgccc tcaggatccg gataagcccg
     1981 tcgagctgcg gctcaccctt tcgtatggca tttgccggga catctgcgtg ccggcggagg
     2041 cccagcttgc gttgacactc cccgccaacg cgactgttcc agcctcgcca gagattgcca
     2101 acgcactcgc agccgttccc cgcggccctg acgagcggca gcctgacgat ccggagttgg
     2161 taacgagcaa ggcccacctc gacggcgatc ggccgaagct gatgctcgaa gcccgtttcc
     2221 ctgggtcggc agcctccggc gatatgttcg tcgaggcgat ggacggtgcg tacgtaccgc
     2281 tacccaccag agttgcggag aagggtgaca cgatcagctt cgaggtggat ctgtcggaag
     2341 ccacgcccaa ggagctgcgg ggcaagacac tgcgcatcac gatggtttcc gatgcaggcc
     2401 agtcggaggc ctattggacg atcgactgac caaggatggt ggcccatact gcttcttgat
     2461 ccgctcgtga cggcgtatag ggtcagggcc atacgtttcg caggttcgct gaacgatcaa
     2521 cccggaggaa tatcatgacg ataaaggtgg gcgaccggct gccggacgcg actttcacca
     2581 taatgggtcc ggaggggccg aagcaggtgc cgacgaatga ggtgttcggc ggcaagaagg
     2641 tggcgctgtt cgccgttccc ggtgcctata cgccgacctg ccacaagcag cacatgccgg
     2701 gtttcgtcgc caatgcagac gagttcaaga acaagggcat cgacatgatc gcctgcacgt
     2761 cagtgaacga cattttcgtc ctgacacaat gggccaagga caccggcgcc gagggcaaga
     2821 tcacgatgct gtcggacggc aatgcggatt tcgtccgcaa gatcggcctt gatatcgatc
     2881 tatctgcccg tggcctcggt ttgcgttcca agcgctattc gatgatcgtc gacgattgcg
     2941 tagtcaaagt gctcaatgtc gaggacgcgc cgccacagca cgacaaatcc agcgccgcga
     3001 acctg
//
LOCUS       DASUMG010000533         2288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_714570, whole genome shotgun sequence.
ACCESSION   DASUMG010000533 DASUMG010000000
VERSION     DASUMG010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714570"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="VFX46_08905"
     CDS             complement(<1..2252)
                     /locus_tag="VFX46_08905"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX5600743.1"
                     /translation="MTHLNESAAIRTPDQRLRVFVSSTLTELAEERVAVSRAISALGL
                     TPVMFELGARPQPPQELYRAYLAQSDIFIGLYWQRYGWIGPGKDISGLEDEFRLSQSM
                     PRLLYVKTPAPDREARLTAMIGELDTQGTVSYRMFRSPRELGRLVRNDLAVLLSERFA
                     AGGSGEDRSPSPEASSSRRVPRSLPMASTSLIGREHDIALVSKLLETPGVRLVTLTGP
                     GGIGKTRLAVAVGAHLEDRYPQGVAFVPLVSIARPELVLPHVAAVLGASIEGTRPPAD
                     VVAERIGKTPTLLVLDNLEQVVGVGPELDQLLARCEELKMLTTSRTALRLRAEHEYQV
                     DPLTVPTFAGRPPIEELVSLPAVQLFVARARAVRHDFVLTEDNAVAVAEICRRLDGLP
                     LAIEIAAARIRLLIPDALLARLGGSLQALGTGPADLPERQRTLRATVEWSIGLLDDVE
                     RQMLATLSIFVEGWTVEAAVHVSGLPEQRTLDLLDALAGHSLVGIDMKDDGPRFRMLA
                     SIRELAAERLAVGADQADVERRHAEYFRALVENADWPAEQQAEWAERLRIEEGNLGIA
                     VRWFFTHNISPLPHMFRILWLFWQMRDRMPEGRAWIRELLTRTNELDGHAQVELLLLS
                     AVTAVEVGDDDGALAAFERLERLDGRIADPYLKSAARLAVSWIRPIVDDFNGALEAAQ
                     AALDGFHRQNEPFTAWAALTVGLLELRLGRHEAARAHLAETSELGGRFGNHWLASVAR
                     TQLTLLAVRTG"
BASE COUNT          441 a          761 c          716 g          370 t
ORIGIN      
        1 cccgtccgca ccgcaagcaa ggtgagctgc gttcgtgcga ccgacgcgag ccagtggttg
       61 ccgaaccgac cgccgagctc gctggtctcg gcgaggtgcg cgcgcgcagc ctcgtgccgg
      121 ccaagcctca gctccagcaa cccaaccgtc aaagccgccc acgccgtgaa cggctcgttc
      181 tgccgatgga aaccatcgag cgctgcctgg gcggcctcga gcgcgccatt gaagtcatcg
      241 acgattggcc ggatccacga gacagccagc cgcgccgcac tcttgagata cggatcggcg
      301 atgcgcccat cgagtcgttc caacctctcg aaagcggcca gcgcaccatc gtcgtcgcca
      361 acctcgactg ccgtgaccgc cgaaagtaac aacagctcga cctgcgcatg cccatccaac
      421 tcgttggttc gggtcagcag ctcccggatc caggcccggc cttccggcat gcgatcgcgc
      481 atctgccaga aaagccagag gatccggaac atgtgcggca gcggcgagat gttatgggtg
      541 aagaaccagc gaacggcgat ccccagattc ccttcctcga ttcgcagacg ctcagcccat
      601 tcggcctgtt gctcagcggg ccagtcggcg ttctccacga gcgcacgaaa gtactcggcg
      661 tgccgacgct caacatccgc ctggtccgcg ccgactgcaa gacgctcggc ggccagctcc
      721 cgtatggatg cgagcatgcg aaacctcggt ccatcgtcct tcatgtcgat accgacgagg
      781 ctgtgcccag cgagggcatc gagcagatcg agtgttcttt gttcggggag accggagacg
      841 tgtacggctg cctcgaccgt ccaaccctcg acgaagatcg acagcgtggc cagcatctgc
      901 cgctccacgt cgtcgagcag cccgatactc cattcgacgg tcgcgcgcaa tgtgcgttga
      961 cgctcgggga gatcggccgg gccggtaccc aaagcctgga ggctgccgcc gagccgagcc
     1021 agcaatgcat ctggtatcag gagtctgatg cgagcggcag cgatctcgat ggcaaggggc
     1081 aggccgtcga gtcgcctaca gatctcggcc acggcgaccg cattgtcttc ggtcaggacg
     1141 aagtcgtgac ggacggcccg ggctcgagcg acgaacagct gaacagcggg caatgagact
     1201 agctcctcga tcggcgggcg acctgcgaac gttggcaccg taagaggatc gacctggtac
     1261 tcatgctcag cgcgcaagcg aagcgcggtg cggctcgtgg tgagcatctt gagctcctcg
     1321 cagcgggcta gcagctggtc gagctccggc cccacaccga ctacctgttc gaggttgtcc
     1381 agcactagta gggtgggcgt tttcccaatg cgctcggcca cgacgtcggc gggtgggcgc
     1441 gtgccctcga tggacgcgcc caaaacggcc gcgacgtggg ggagaaccag ctcgggccga
     1501 gcaatcgata cgagcggcac gaaggccacg ccttgcggat agcgatcttc caggtgcgca
     1561 ccaaccgcga cggccagcct ggtcttgccg atgcctccag gacctgtgag ggtgaccagc
     1621 cgaacccccg gcgtctccaa tagcttggag accagcgcaa tgtcgtgctc gcgaccgatc
     1681 agtgaagtgg atgccatcgg caatgaacgc ggaacgcgtc ggctgctgct cgcctcgggc
     1741 gatggtgacc gatcctcgcc gctgcccccc gccgcgaacc gctcgctcag aagcactgcg
     1801 aggtcgttcc ggaccaatcg ccccagctcc cgcggacttc ggaacatacg gtaagagact
     1861 gtcccctggg tatcgagctc gccgatcatg gcagtcagcc gcgcttctcg atcgggtgcc
     1921 ggtgtcttga catacagcag acggggcatc gactgagaga gccgaaactc gtcctccagg
     1981 cccgagatgt ccttacccgg accgatccag ccgtaacgct gccagtacag cccaatgaaa
     2041 atgtcggatt gggccaggta ggctcgatac agctcctgcg gaggctgcgg tcgagccccg
     2101 agctcaaaca tcactggtgt aagcccgagc gccgagatgg cgcgcgacac cgcgacgcgt
     2161 tcctcagcca gctcggtcag cgtcgagctc acgaagacgc gcagacgctg atccggggtt
     2221 cggattgcag cgctctcatt caagtgggtc atatatcagt cctcgaaggc atgacccatg
     2281 caagacgc
//
LOCUS       DASUMG010000534         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_473763, whole genome shotgun sequence.
ACCESSION   DASUMG010000534 DASUMG010000000
VERSION     DASUMG010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473763"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VFX46_08910"
     CDS             complement(<1..1290)
                     /locus_tag="VFX46_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600744.1"
                     /translation="MWTGRQAFERIENAIVSLHRQENELDSALASATAEAERLRRERS
                     EAFRELARIKLDEITAKRLVEDLDAAEARAVRLLERRRSELDQVAADRQAALAALEQA
                     EAERSAAAAALEAAIDAVDDLRAKVAEEIQSSEEWQQAKAAYESARKIAGAADAKAAQ
                     SEQELAEKRKPYDEDPLFFYLWSIGYLTPRYKAGPFARFMDGLTAKHIGFADARLNYA
                     MLIQIPERLREHARLQQEIADSQKRALEEVERKALSAAGEDELVRKMEEARRKLAEAD
                     GAVERAQAALRATDERRDALIASLEDETYREALNIIAMADAQDRLETLVQEAKRTPTP
                     ADDAMVQRIGELNERIERIDAEIAELRKSARSIAERRLEVERARDRFRKSGYDHPDVI
                     FDNELDLERILGQILGGAATGSVLWDILRAGFGLRYPG"
     gene            complement(1308..2249)
                     /locus_tag="VFX46_08915"
     CDS             complement(1308..2249)
                     /locus_tag="VFX46_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6384 family protein"
                     /protein_id="HEX5600745.1"
                     /translation="MDHSLQESKPHTLDEVMMAMDVVDTLRHREELVRRELDEAGREA
                     ELIARLREIYREQGIEVPDRVLEQGVKALKDQRFVYTPPPPSWKRTVLELWAQRDKVG
                     KRILKVLLILLLLAGLAYFALIRPAQEARRQALIEATETLPRSIRVTHSDIQQIATEE
                     AARSRADALLADGERAIRDGDVPEMRRISAELQQLRDTLSAEYTLRIVSEPGQVTGFW
                     RRPPGLWSQRSYYLVVEPITPDGRRLSLPVRDEVTGETQTVSQFGVRVPRETYEAVAE
                     DKRQHGIVQRNEFGVKRRGRTQVEYLMPFEGGTVTSW"
     gene            complement(2275..>2639)
                     /locus_tag="VFX46_08920"
     CDS             complement(2275..>2639)
                     /locus_tag="VFX46_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEX5600746.1"
                     /translation="DSVLTALKASFTGVFGLHESTKTLDAMKEGMSKSLEDLAKIGEK
                     VQEEAIRAGYGPTIRADAVKQLVDSVINFQERSREIISEMRKLATQNSQEIRDAVEDG
                     KRRIARLAAEAEVLPALR"
BASE COUNT          433 a          891 c          759 g          556 t
ORIGIN      
        1 gcccggatat cgcaggccga aaccagcacg caaaatgtcc caaagcacgc tgccagttgc
       61 ggcgcctccc aggatttgac cgaggattct ttccagatcc agctcgttgt cgaagatgac
      121 gtccggatga tcgtagccgg acttgcggaa ccggtcccgc gctctttcca cctctagccg
      181 ccgctccgca atagagcgcg ccgacttccg caactcggca atttctgcat cgatccgctc
      241 gatgcgttcg ttcagctcac cgatccgctg caccattgcg tcgtcagcag gcgtgggcgt
      301 gcgcttggcc tcctggacca gcgtttccaa gcggtcttgc gcgtcggcca ttgcaatgat
      361 gtttagggct tcgcgatagg tctcgtcctc caagctcgcg atgagcgcat ctcgcctttc
      421 atcggttgca cgaagcgccg cttgggcgcg ctcgacggcc ccgtcagcct cggccagctt
      481 gcgccgtgcc tcctccatct tgcgaacgag ctcatcctcc cccgcagcgc tgagtgcttt
      541 tcgctcgact tcttcaagcg ccctcttttg gctgtccgca atctcctgtt ggagccgcgc
      601 gtgctcgcgc agtcgctccg ggatctggat gagcattgcg tagttcaggc gggcatcggc
      661 aaatcctatg tgctttgccg ttagaccgtc catgaagcgg gcgaacggcc ctgccttgta
      721 gcgcggggtc aggtagccga tggaccaaag gtagaaaaac agcggatcct cgtcgtaggg
      781 cttacgtttt tcggcgagct cttgctcaga ctgagccgct ttggcatcgg cggccccggc
      841 gatctttctc gccgattcgt aggccgcctt cgcctgttgc cactcctcag acgactggat
      901 ctcttccgca accttcgccc tcaagtcgtc gacggcatcg atagcggctt ccaacgcggc
      961 agccgccgcg ctgcgctccg cctccgcttg ctcgagtgcg gcaagcgccg cctgccgatc
     1021 tgccgcgact tggtcgagct cactgcgccg gcgttcgagt agccgcacgg cgcgcgcctc
     1081 agcggcgtcg agatcctcga ccagacgctt cgccgtgatc tcgtccagct tgatccgagc
     1141 cagctcccga aaggcttcac tgcgctcgcg acgcagccgt tcggcctcgg ctgtcgcaga
     1201 cgcaagcgcg ctgtcgagct cgttctcctg gcgatgcagg ctgacaattg cgttctcgat
     1261 gcgctcgaaa gcctgtcgtc cggtccacat gggcccgatg tccttcgcta ccagctcgtc
     1321 actgtcccgc cctcgaaggg catcaggtac tcgacttgcg ttcgaccccg gcgcttcacg
     1381 ccgaattcgt tccgctgcac gatcccgtgc tgacgcttgt cttcggctac agcttcgtag
     1441 gtctctcggg gaacacgaac accgaactgg ctgacggtct gcgtctcgcc agttacttca
     1501 tcgcggacgg gaagtgataa tcggcgaccg tcgggcgtga tcggctccac gaccaagtag
     1561 tagctgcgct gcgaccacag tcccggaggg cggcgccaaa atccggtcac ttggcccggc
     1621 tcggagacga tccttagcgt atactctgcg ctcaaagtgt cccgcagctg ctgaagctca
     1681 gcactaatgc ggcgcatctc tggaacgtcc ccatcgcgga tggcgcgctc accgtccgca
     1741 agcagcgcat cggccctgct gcgcgccgcc tcctccgtcg cgatctgctg aatgtcagag
     1801 tgggtgacgc ggatcgacct aggcaatgtc tcggtcgcct caatgagagc ctgacggcgg
     1861 gcctcctgag ccggtcggat caaagcaaag taggcgagcc cagccagcaa gagcaggatt
     1921 agcagtacct tgaggatccg cttgccgact ttgtcacgct gcgcccacag ctcgagcaca
     1981 gtgcgtttcc acgagggcgg tggcggtgtg tagacgaagc gttgatcttt cagggctttg
     2041 acaccctgct cgagcacgcg gtccggaacc tcgattcctt gctctcggta gatttcccga
     2101 agccgggcga tcaactccgc ttcccgccct gcctcatcca gctcgcggcg caccagctcc
     2161 tcgcgatggc gcaacgtgtc aaccacgtcc atcgccatca tgacctcgtc caacgtgtgc
     2221 ggtttggact cttgcagact atggtccacc gtgcgcccct atggacctat ccagctagcg
     2281 aagcgcaggc aggacctccg cttccgctgc gagacgggca atgcgccgct tgccatcctc
     2341 cacagcatcg cggatttcct gcgagttctg ggtggccagc ttgcgcattt ccgaaatgat
     2401 ctcccgcgac cgctcctgga agttaataac gctgtcaacg agctgcttga ctgcatcagc
     2461 gcggatcgtg gggccgtacc cagcccggat cgcctcctcc tggacctttt caccgatctt
     2521 ggccagatcc tcgaggctct tcgacatgcc ttccttcatt gcatcgaggg tcttggtgga
     2581 ctcgtggagc ccgaaaactc cggtgaagga ggctttgaga gcggtcaaga cgctgtcgt
//
LOCUS       DASUMG010000535         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_875729, whole genome shotgun sequence.
ACCESSION   DASUMG010000535 DASUMG010000000
VERSION     DASUMG010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875729"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFX46_08925"
     CDS             complement(<1..647)
                     /locus_tag="VFX46_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600747.1"
                     /translation="MVGRGCGAWLAALAALLVGGCSYEHALGVDHYSVFKVDPPRNRT
                     VTVCHAYGCRQKTRFTFTQADITELKRLMEKTKKADTPHEERRAIAYAIGWMERRTGE
                     VVGTKNDRPGMDFAGSGDPTQQDCVDEATNTTSYLTVLQNEGLLKHHTVGQPFAKENY
                     LRGVAGWTHWTATIYEKESGVRWAVDSWIYENGENPAVVKANEWYVASINELPTAT"
     gene            787..2205
                     /gene="trmFO"
                     /locus_tag="VFX46_08930"
     CDS             787..2205
                     /gene="trmFO"
                     /locus_tag="VFX46_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5600748.1"
                     /translation="MATAKPVHVIGGGLAGSEAAWQIARAGIPVVLHEMRPVRGTEAH
                     KTDGLAELVCSNSFRSDDWETSAVGLLHEEMRRAGSLIMAAGDRHKLPAGGALAVDRE
                     GFSAEVTARLTALPNVTIERGEVAGPPPDEWDNVIIATGPLTSPALSQAILELTGEDA
                     LAFFDAIAPIVHYDTIDMSVAWRQSRYDKAGPGGTGADYINCPLDKEQYERFVDALLA
                     GEKTEFREWEKNTPYFDGCLPIEVMAERGRDTLRYGPMKPVGLSNPHKGGERPYAVVQ
                     LRQDNALGTLWNMVGFQTKLKYGEQVRIFRMIPGLENAEFARLGGLHRNTFLNSPKLL
                     DGLLRLKAQPRLRFAGQVTGVEGYVESAAIGLLAGRFAAAERLGRKPVPPPPTTAFGA
                     LLGHITGGHLAEDRIEAGPRSFQPMNINFGLFPPLEPGAEGARLKGKERGRAKKRAIA
                     LRALQDVDRWLTDVTLPRAAAE"
     gene            complement(2239..>3117)
                     /locus_tag="VFX46_08935"
     CDS             complement(2239..>3117)
                     /locus_tag="VFX46_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600749.1"
                     /translation="LVKCDAEFARKIDLLMVSIGGNDVGFSRLVANAVLTDKSMLKWL
                     GGWLGQIEGASTAKDQLARLYWRYRALHRALQHVLHIQESDRILLTAYPGLALLEDGT
                     TVCPSGRAGMDVLKDFKLDEKKAREGSWLAEHLNELMRRAAREHGWTFVESHRKEFLG
                     RGICAGWSDAAFSTADDLRLPRKIDGVWKPYNPADYRPYAPRQRWFRTPNDAFMTGNF
                     HVPGTLLQKVLQMHTSWFQLVLASTYSGAFHPTAEGQAAIADAVVTKARQVLAKYTAG
                     NEMQHGFYTDVPRFNP"
BASE COUNT          553 a         1012 c          965 g          587 t
ORIGIN      
        1 gtcgccgtcg gcagctcgtt gatcgaggca acgtaccact cgttggcctt cacgacggcc
       61 ggattttccc cgttctcgta aatccagctg tcaaccgccc agcgcacccc ggattccttc
      121 tcgtagatgg tggcggtcca gtgggtccag cccgcgactc cgcgcaggta gttctccttg
      181 gcgaacggct ggccgaccgt gtggtgcttc aggaggcctt cattctgcag gacggtgagg
      241 tagctcgtgg tgttggttgc ctcgtccacg cagtcctgct gtgtgggatc accggagcca
      301 gcgaagtcca tgcctgggcg atcgttcttg gtgccaacca cctcgccggt acgccgctcc
      361 atccagccga tggcataggc gatggcacgg cgctcctcgt gcggcgtgtc cgcctttttc
      421 gtcttttcca tcagcctctt gagctcggtg atgtcggcct gtgtgaatgt gaacctggtc
      481 ttctgccggc agccataagc gtggcaaaca gtgacggtcc tgttgcgcgg cgggtcgacc
      541 ttgaagacgg aatagtgatc aacgcccaag gcgtgctcat acgagcagcc gccaacgagc
      601 aatgcagcca gggctgccag ccaagcccca cacccccgtc caaccatttc cctccgaccc
      661 caccagcagc aatccgtcgc ggccgcctgt gctctcggca caattccttc cttactatct
      721 caatgaatcg attgagaata cccagcgcgt tgagggtgcg aggcttttcc gatacgttgg
      781 agcactatgg ccacagcgaa gccagttcat gtgatcggcg gcgggcttgc aggctccgaa
      841 gcagcctggc agatcgcgcg cgccggcatt cccgtcgttc ttcatgagat gcggcctgtc
      901 cgcgggaccg aggcccacaa gaccgatgga ttggcggagc tcgtctgctc caattccttc
      961 cgctcggacg actgggagac gagcgcggtc gggcttttac acgaggagat gcgccgcgcc
     1021 ggctccctca tcatggctgc cggggaccgg cataagttac cagccggcgg agcgctggcc
     1081 gtagatcgcg aaggcttttc tgccgaggtg accgcgcggc tcacagctct gccaaacgtc
     1141 acgatcgagc gtggcgaggt cgcggggccg ccgccggacg agtgggacaa cgtcattatc
     1201 gcgacgggac cgctgacatc gcccgccttg agccaagcta tcctcgagct gacaggtgag
     1261 gatgccctcg ccttcttcga tgccatcgcg ccaatcgttc actacgacac gatcgacatg
     1321 tccgtcgcct ggcggcagtc gcgctatgac aaggccggtc ctggagggac cggagccgac
     1381 tacatcaact gcccgctcga caaggagcag tacgagcgct tcgtcgacgc actgcttgcc
     1441 ggtgagaaaa ccgagttccg cgagtgggag aagaacaccc cctacttcga cggctgcttg
     1501 cccatcgagg tcatggccga gcgcgggcgc gatacgttgc gctacgggcc gatgaaaccg
     1561 gtcggccttt ccaatccaca caagggcgga gagcggccgt acgcggtggt gcagctgcgt
     1621 caggacaatg cgcttggcac gctctggaac atggtcggct tccagacgaa gctgaagtac
     1681 ggcgagcagg tgcgcatttt ccgcatgatc ccggggctcg agaatgctga gttcgcgcgg
     1741 ctcggcggcc tgcatcgcaa cacgttcctc aacagcccca agctgctcga tgggctgttg
     1801 cggctcaagg cgcagccgcg gttgcgcttt gccggtcagg tcacaggtgt cgagggctac
     1861 gtcgagagtg ccgccatcgg gctccttgct gggcgctttg ccgccgcgga gcggctgggc
     1921 cgcaagcctg tgccgccgcc gccaacaacg gcgttcggcg cgctgctcgg gcacattact
     1981 ggcggccact tggctgagga tcgcattgaa gccggcccgc gctcgttcca gccgatgaac
     2041 atcaatttcg gactgtttcc gccgcttgaa ccgggcgcag aaggcgcacg tctcaagggc
     2101 aaggagcgcg gccgtgcgaa gaagcgggcc atcgccctgc gggcgctcca agacgtcgac
     2161 cgctggttga ccgacgtcac ccttccccgc gccgcggcgg aataggagcg ccgccctacg
     2221 ctttagaccg aagagcagct acggattgaa ccgcggaacg tcggtataga atccgtgctg
     2281 catctcgttg cccgccgtgt acttggcgag cacttggcgt gccttcgtga caaccgcatc
     2341 tgcgatagcc gcctggcctt ctgcagtcgg atggaacgcg cccgagtatg tcgaggccag
     2401 caccagctga aaccaggacg tatgcatctg cagcaccttc tgcagcagtg tgcctggaac
     2461 gtggaaattg ccggtcatga acgcgtcatt cggcgtcctg aaccagcgct gacggggggc
     2521 gtaggggcga tagtcggctg ggttgtatgg cttccatacc ccatcgatct tgcgcggtag
     2581 tctgaggtcg tcggccgtcg agaaggccgc gtcgctccaa cccgcacaga ttccacgacc
     2641 gaggaactcc tttcggtggc tttcgacaaa cgtccagccg tgctcccgag cagcccggcg
     2701 catcagctcg ttgagatgct ccgccagcca actgccctca cgcgctttct tctcgtccaa
     2761 cttgaagtct ttgagcacat ccatgcccgc tcggccactg gggcaaaccg tggtcccgtc
     2821 ctcgagcagc gccagccccg gataggcggt cagcagaatc cggtcgctct cctggatgtg
     2881 aaggacatgc tgcagcgccc gatgaagtgc ccgatagcgc caatagagac gtgcgagctg
     2941 gtccttggcg gtgctcgcac cctcaatctg accgagccac ccgccaagcc acttcagcat
     3001 cgacttgtcc gtcagcactg cgttggcaac aagccgcgag aagccgacgt cgttgccgcc
     3061 gatcgagacc atcagcaagt cgatcttccg tgcgaactcg gcatcgcact tgacgag
//
LOCUS       DASUMG010000536         2423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_795998, whole genome shotgun sequence.
ACCESSION   DASUMG010000536 DASUMG010000000
VERSION     DASUMG010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795998"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1618
                     /gene="groL"
                     /locus_tag="VFX46_08940"
     CDS             35..1618
                     /gene="groL"
                     /locus_tag="VFX46_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109730.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX5600750.1"
                     /translation="MMRGVDILANAVKVTLGPKGRNVVIEKSFGAPRTTKDGVTVAKE
                     IELEDKFENMGAQMVREVASKTNDVAGDGTTTATVLAQAIVREGLKAVAAGMNPMDLK
                     RGIDKAVAQAVEEIQKRAKKVKSSDEVAQVGTISANGESEIGKMIAEAMQKVGNEGVI
                     TVEEAKSLETELEVVEGMQFDRGYLSPYFITNADKMVAELEDPYILIHEKKLSGLQAM
                     LPVLEAVVQTGKPLLIIAEDVEGEALATLVVNKLRGGLKVCAVKAPGFGDRRKAMLED
                     IAILTGGTVISEDLGIKLENVTLDMLGRAKRVTIDKDNTTIVDGAGKKKDIEARVAQI
                     KVQIEETTSDYDREKLQERLAKLAGGVAVIKVGGATEVEVKERKDRVDDALNATRAAV
                     EAGVVPGGGVALLRAAAAINVKGENEDQDAGIQIIKRALQAPIRQIAENAGVEGSIVV
                     GKVLESKSYNFGYDAQNDEYGDLIEKGIIDPAKVVITALQDAASVAGLLITTEAGVAE
                     APKKKEAPAAPGMGGMDMM"
     gene            complement(1738..>2423)
                     /locus_tag="VFX46_08945"
     CDS             complement(1738..>2423)
                     /locus_tag="VFX46_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX5600751.1"
                     /translation="DGAISPELRARFLGLKSGSGSFRMNVALSELPDFLCLPGKEPQA
                     HHGSGIIIGPTISYLERAYIDARLHGWSREPIIEMLIPSTLDPSLAPSGRHVASLFVQ
                     HVAPHLPDGRSWDEAKEEFADLVIDTVNRYAPNFKASVIARQVLSPLDLERRFGMVDG
                     DIFHGQLSLDQLFFARPVLGYADYRMPVPGLYLCGSGAHPGGGVTGVPGHNAAREIIR
                     DLRGWLRAH"
BASE COUNT          493 a          728 c          822 g          380 t
ORIGIN      
        1 agaggtcaag ttcgctgcag atgcgcgtga gcgcatgatg cgcggcgtag acatcctcgc
       61 gaacgctgtg aaagtgacgc tcggcccgaa gggtcgcaac gtcgtcatcg agaagtcctt
      121 cggtgccccc cggaccacga aagacggtgt cacggtcgcc aaggagatcg agcttgagga
      181 caagttcgag aacatgggcg cccagatggt ccgcgaggtc gcatccaaga cgaacgacgt
      241 cgcgggtgac ggcaccacga ctgcaaccgt tcttgctcag gcgatcgtcc gcgagggcct
      301 gaaggccgtt gccgccggca tgaacccgat ggacctgaag cgcggcatcg acaaggcggt
      361 cgcacaggcg gtcgaggaga tccagaagcg cgccaagaag gtgaagagct cggacgaggt
      421 cgcgcaggtc ggcaccatct ctgccaacgg cgagagcgag atcggcaaga tgatcgccga
      481 ggccatgcag aaggtcggca acgagggcgt gatcaccgtc gaggaggcga agagcctcga
      541 gaccgagctt gaggtcgtcg agggtatgca gttcgatcgc ggctacctct cgccgtactt
      601 catcaccaac gccgacaaga tggtggccga gctcgaggat ccgtacatcc tgatccacga
      661 gaagaagctg tcgggcctgc aggccatgct gccggtgctt gaggccgtcg ttcagaccgg
      721 caagccgctg ctcattatcg ctgaggacgt tgagggtgag gctctggcca cgctcgtcgt
      781 caacaagctg cgcggcggcc tgaaggtctg cgctgtgaag gctccgggct tcggcgatcg
      841 ccgcaaggcc atgctggagg acatcgcgat cctcacgggc ggcacggtca tctccgaaga
      901 cctcggcatc aagctcgaga acgtcacgct tgacatgctg ggccgcgcca agcgggtgac
      961 catcgacaag gacaacacaa ccattgtcga cggcgccggc aagaagaagg acatcgaggc
     1021 ccgcgtcgcc cagatcaagg tgcagatcga ggagaccacg tccgactatg accgcgagaa
     1081 gctgcaggag cgcctggcga agctcgctgg tggcgttgcg gtgatcaagg tcggcggtgc
     1141 gaccgaggtc gaggttaagg agcgtaagga tcgcgtcgac gacgcgctga acgccacccg
     1201 cgccgctgtt gaggctggcg ttgttccggg cggtggtgtt gcgctgctcc gggccgctgc
     1261 ggccatcaac gtcaagggcg agaacgaaga tcaggatgcc ggcatccaga tcatcaagcg
     1321 ggctcttcag gctccgatcc gccagatcgc cgagaacgcc ggcgtcgagg gctcgatcgt
     1381 ggtcggcaag gtgctcgagt cgaagtcgta caacttcggc tacgatgcgc agaacgacga
     1441 gtacggcgac ctcatcgaga agggcatcat cgacccggcc aaggtcgtga tcacggccct
     1501 ccaggatgct gcttccgtcg ctggtctgct catcaccacg gaagctggcg tggccgaggc
     1561 tccgaagaag aaggaggctc ccgctgctcc cggcatgggc ggcatggaca tgatgtaatt
     1621 caggtctccc cagaccttgg acggaggggc cgtaccgggc gaccggcgcg gccctttctc
     1681 tttccggcaa ggctttcgga agtcggcacc caggttgcgg cgagcagacc gctgccgtca
     1741 gtgtgcccta agccaacccc gcagatcgcg aatgatttcg cgcgctgcat tatgacccgg
     1801 gacgccggtg acgcccccgc ccgggtgagc ccccgagccg cacaggtaca ggccgggtac
     1861 tggcatccgg taatcggcgt agccgagcac agggcgcgcg aagaagagct gatcgagcga
     1921 caactggccg tggaagatgt cgccgtcgac catgccgaag cggcgttcta gatcgagcgg
     1981 cgatagcacc tgccgggcga tcacacttgc cttgaagttc ggtgcgtagc ggttgacggt
     2041 atcgatgacg agatcggcga actcttcttt cgcctcgtcc cagctccggc catcgggcag
     2101 atgcggggcc acgtgctgga cgaacaggct cgcgacgtgc cggcccgatg gggcgagaga
     2161 cggatcgagc gtcgagggga tgagcatttc gatgatgggc tcgcgcgacc agccatgcag
     2221 acgcgcatcg atgtaggccc gctccagata ggaaatggtc ggcccaatga tgatgccgga
     2281 gccatggtgc gcctgcggct ccttacccgg taggcaaaga aaatcgggta gctccgacag
     2341 cgccacgttc atccgaaagc tgcctgagcc cgatttcaag ccgagaaaac gcgcccgcag
     2401 ctcgggtgaa atggccccat ccg
//
LOCUS       DASUMG010000537         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_73676, whole genome shotgun sequence.
ACCESSION   DASUMG010000537 DASUMG010000000
VERSION     DASUMG010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73676"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFX46_08950"
     CDS             complement(<1..491)
                     /locus_tag="VFX46_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX5600752.1"
                     /translation="MSDDLPLAAEFPPTTRDDWRRLVETALKGASFDKRLVSQTYDGL
                     RVEPLYPRAASASAVAGRFAVPWQVIQRVDHPDAKTANKQALDDLENGATGLSLVFAG
                     SVGAYGYGLDGTKEMIATVLEGVHLDAGIALELDLSAQYKDAGQNLATVLKERGIAPN
                     RTQ"
     gene            complement(534..1016)
                     /gene="folK"
                     /locus_tag="VFX46_08955"
     CDS             complement(534..1016)
                     /gene="folK"
                     /locus_tag="VFX46_08955"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEX5600753.1"
                     /translation="MPEALLALGGNVGNSRVILDRAIGLLCDGKDVRLIARSSDYRTP
                     PWGFKYQPHFVNLAIIVETTLGPHELLARAQEVEIRLGRDRVREKRNGPRTADIDILA
                     YDDLTLNEPNLTLPHPRLFERAFVLIPLAEIAGDRVIAGRRVDEAARVVDAGQIHRLP
                     "
     gene            complement(1009..1377)
                     /gene="folB"
                     /locus_tag="VFX46_08960"
     CDS             complement(1009..1377)
                     /gene="folB"
                     /locus_tag="VFX46_08960"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEX5600754.1"
                     /translation="MSDRIFVKGLALHAYHGVMPHESKVGQTFIIDLDLEIDLSAAAR
                     SDKVVDTVSYDKVVDCVSKAFCAQRLRLIEAAAGRTADAVLATFPRVRSVRITIHKPH
                     APIAATFSDVGVTLVRSRHA"
     gene            complement(1374..2141)
                     /gene="folP"
                     /locus_tag="VFX46_08965"
     CDS             complement(1374..2141)
                     /gene="folP"
                     /locus_tag="VFX46_08965"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX5600755.1"
                     /translation="MGILNVTPDSFSDGGRYFEPGKAVAHARDMAAQGADIIDIGAES
                     TRPYGSPVPVSADDEKTRLATVLPAVVAIGVPVSIDTIKASIVAWALDQGAAIVNDVW
                     GLQRDPDMAPLVAKRGAPVIVMHNRESADPGIDIVADVNNFFSRSLEIAASAGIPRER
                     IVLDPGIGFGKTPEQSITCIARLAEFKRFGLPMLVGASRKRFISTVTPSTPDERIGGS
                     ISAHLLAVEKGAAIIRAHDVAETVQALRVAAAIEAAR"
BASE COUNT          419 a          672 c          719 g          437 t
ORIGIN      
        1 atttgcgttc tgttcggcgc gataccgcgc tccttcagca ctgtcgccag attttgtccg
       61 gcatctttgt actgggcgct gagatcgagt tcgagcgcga tgccggcgtc gaggtggacg
      121 ccctcgagca cggtggcgat catttccttc gtgccatcga ggccatatcc ataggcgccg
      181 acgcttccgg cgaagaccag gctcaatccg gttgcgccgt tttcgaggtc gtccagagcc
      241 tgcttgttgg cggtctttgc gtcgggatgg tcgacccgct ggatcacctg ccacgggaca
      301 gcgaaacggc cggcgaccgc agatgctgac gccgcgcggg gatagagtgg ctcaaccctc
      361 agcccgtcat aggtctggct cacaagcctc ttgtcgaacg aggcaccttt aagggcagtc
      421 tcgaccagtc tgcgccagtc gtcccgggtg gtcggcggga actccgctgc gaggggtaaa
      481 tcgtcactca taatggcaaa atggcacagt cggccggacg gggctatcga ccgttagggc
      541 agacgatgga tctggccggc atctaccaca cgggcggctt catcgacgcg ccgaccggcg
      601 atcacgcggt ctcccgcgat ctcggccagc ggtatcagca cgaaagcacg ctcgaacaag
      661 cgcggatgcg gcagggtcaa attcggctca tttaaagtca ggtcgtcata agcgaggatg
      721 tcgatatcag cggtgcgtgg gccgttgcgc ttttcgcgaa cgcggtcgcg gccgagtctg
      781 atctctacct cttgggcgcg tgccagcagc tcatgtgggc cgagtgtcgt ttcaacaatg
      841 atcgcgaggt tcacgaaatg cggttgatat ttgaagcccc atggcggcgt tcggtagtcg
      901 gacgaacgag ctatcaggcg cacgtctttt ccgtcgcaga gcagtccgat agcgcgatcg
      961 agaataacgc ggctgtttcc gacattgcca cccagcgcca gcagcgcttc aggcatgacg
     1021 gcttctcaca agcgttaccc cgacgtcgct gaaggtggcc gcgatcgggg cgtgcggctt
     1081 gtgtatcgtg atgcgcacgg atcgcacgcg cgggaaagtt gcgagcacgg cgtcggcggt
     1141 gcggccggcg gcggcctcta tcaggcgaag acgttgggcg caaaacgctt tgctcacgca
     1201 atcgacgacc ttgtcgtagg agacggtatc gacgaccttg tccgagcgcg cagcggctga
     1261 aaggtcgatc tccaggtcga gatcgatgat gaaggtctgc cccaccttgg attcgtgcgg
     1321 catcacgccg tgataggcgt gaagagcaag acctttgacg aaaatccgat cgctcatcgt
     1381 gcggcctcga tggcggcggc tacccgtagc gcttgcacgg tctccgcgac gtcatgtgcg
     1441 cggatgatcg cggctccctt ttcaaccgcg agcagatggg ccgagatcga gccaccgatg
     1501 cgctcatccg gtgtcgatgg cgtcacggtg ctgatgaacc gtttccgcga tgccccaacg
     1561 agcatgggta ggccgaagcg tttgaactca gcaagccggg cgatgcaagt gatactttgc
     1621 tcaggtgtct tgccgaagcc gatcccggga tccaggacga tacgctcacg cgggatgccg
     1681 gcactggcag cgatttccaa cgagcgtgaa aaaaagttgt tcacgtcggc aacaatgtcg
     1741 atgcccggat ccgccgactc gcgattgtgc atcacgatga ccggcgcgcc gcgcttggcg
     1801 acaagtggtg ccatgtcggg gtcacgttgc agaccccaca cgtcattgac gatcgccgcg
     1861 ccctggtcga gcgcccaggc gacgattgaa gccttgatgg tgtcgatcga caccggcacg
     1921 ccaatcgcga cgactgccgg caataccgtt gcgagccggg tcttctcgtc atcagcggac
     1981 accggaaccg ggcttccata ggggcgggtg gattctgccc ctatatcaat gatatcggct
     2041 ccctgagcgg ccatatcgcg ggcatgcgcg accgctttgc caggctcgaa gtaccgcccg
     2101 ccatccgaga aagagtctgg tgtgacgttg agaatgccca tgacaagcgg gcggcagtgc
     2161 gccagcagtt ccgcgagcac gttggcagcg gtcttttgcg cagatccggc accaatttcg
     2221 agcgttcggg ccattcccat gccggtc
//
LOCUS       DASUMG010000538         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1198246, whole genome shotgun sequence.
ACCESSION   DASUMG010000538 DASUMG010000000
VERSION     DASUMG010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198246"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFX46_08970"
     CDS             complement(<1..259)
                     /locus_tag="VFX46_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX5600756.1"
                     /translation="MDKQQPARILIAEDEPIIALSLQDLLVEAGFGVACVTGRLHKAL
                     ALIEGPGCDAAIVDANLRGVSTGPAALALAVRGVPFIVLSGY"
     gene            complement(252..1805)
                     /locus_tag="VFX46_08975"
     CDS             complement(252..1805)
                     /locus_tag="VFX46_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX5600757.1"
                     /translation="MPDEARTEPMLLAENAELRAQLEGAEETLRAIRNAEVDALIIES
                     DAGPRVYTLHGVDSASNRFRGEILAQVSDAVIAIDGHQRVTYANAAAERLYGLGPSWA
                     LGQLLSKVYETRWLNPGDEAAAETALRKRGEWRGENVHVRQDGRELHVESTMTMLRET
                     VGQSAGVLAVIRDISERKRAEDKVRISEIRYRRLFEAAQDGVLLLDPDTCKITDANPF
                     MTKLLSYAHDELVGKELYEIGLMKDEAASLEMFRKLKSEHQVRYEDLPLRSQGGRHQE
                     VEVVANLYDENGRSVIQCNIRDITERKRVEEHNKLLMAEINHRSMNLLAVVQAVAHQT
                     ARTGDPATFVARLFDRIDGLAASQDLLIKNQWEGVDLADLVEAQLSHFKDLIGARVMR
                     DGPPVRLTPAAAQGIGMALHELATNAGKYGALSNNDGRVHISWQFGTANEPVFLMSWL
                     EEGGPTVALPTRKGFGQMVIGRMVEAAVDGTAEVDYRESGFFWRLSAPLAGTLQKGRL
                     ASSAGGVRG"
     gene            complement(1812..>2112)
                     /locus_tag="VFX46_08980"
     CDS             complement(1812..>2112)
                     /locus_tag="VFX46_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX5600758.1"
                     /translation="AQCAKARYMLRLYITGTTLQSTRAILNIRKICEEHLQGRYELEV
                     VDISRHPMLAIGEQIIAAPTLIKKLPLPLRRFIGDMSQTERILLGLDLRATARSA"
BASE COUNT          349 a          683 c          671 g          409 t
ORIGIN      
        1 aatagcccga cagcacgatg aaaggcacgc cgcgaactgc gagcgccaat gcggcaggac
       61 cggtactcac accgcgtagg ttggcgtcga caatggccgc atcgcagccg gggccttcga
      121 tgagggcgag cgccttatgg agtcttcctg tcacgcaggc aaccccgaaa ccggcttcga
      181 ccagcaagtc ttggaggctg agcgctatga tcggctcgtc ctcggcaatc aggatgcgcg
      241 ccggctgctg cttatccacg gactcctcct gcggacgatg ccaatcgtcc cttctgcaac
      301 gtgcctgcga gcggcgcgct caatctccag aagaacccac tctctcggta gtcgacctcg
      361 gctgtcccgt cgaccgccgc ctcgaccatg cggccgatca ccatctggcc gaaacccttg
      421 cgtgtaggga gggcgaccgt gggaccgccc tcctccaacc aggacatcaa gaacaccggc
      481 tcgttggccg taccgaattg ccaggagatg tgcacccgcc cgtcgttgtt cgagagtgcc
      541 ccgtactttc cagcgttggt ggcgagctca tgcagcgcca tgccgatgcc ctgggccgcc
      601 gccggcgtca ggcgcacggg cgggccgtcg cgcatcacac gcgcgccgat caggtccttg
      661 aaatgggaaa gctgggcttc cacgagatct gcaagatcga cgccctccca ctggttcttg
      721 atcagcaggt cctggctggc ggccagtccg tcgatgcgat cgaacaggcg tgcgacaaag
      781 gttgccggat cgcccgtgcg cgcggtctga tgggcgaccg cctgcaccac ggcgagcagg
      841 ttcatggagc gatgattgat ctcggccatg agcagcttgt tgtgctcctc gacccgcttg
      901 cgctccgtga tatcgcggat gttgcactga atgacggacc ggccgttctc gtcatagagg
      961 ttggcgacca cctcgacttc ctgatgccgt ccaccctggc tcctgagtgg caagtcctcg
     1021 taacggactt ggtgctcgct cttgagcttg cggaacatct ccaggctcgc ggcttcgtcc
     1081 ttcatcaggc cgatctcata cagctccttt ccgaccagct cgtcgtgcgc gtaactcagc
     1141 agcttcgtca tgaagggatt ggcgtcggtg attttgcacg tatcgggatc gaggaggagg
     1201 acaccgtcct gcgccgcttc aaaaagccgg cggtagcgga tttccgagat gcgcactttg
     1261 tcctcggccc gcttgcgctc ggagatgtcg cggatgacgg caaggacgcc agccgactgg
     1321 ccgacggttt cccgcagcat cgtcatggtc gattcgacgt gcagctcgcg gccgtcttgt
     1381 ctgacgtgga cgttctcgcc gcgccattcg ccgcgcttgc gtagggcggt ctcggcagcg
     1441 gcctcgtcgc cggggttgag ccatcgggtc tcgtacacct tcgacaggag ttgtccgagc
     1501 gcccaggacg ggcccaagcc gtatagccgc tcggcggcgg cgttggcata ggtgacgcgc
     1561 tggtggccat cgatggcgat tacggcgtca ctgacctgcg cgaggatctc gccgcggaag
     1621 cggttcgacg ccgagtccac gccatgcagc gtatagacgc gcgggcctgc gtcgctctcg
     1681 atgatgaggg cgtcgacctc agcgttgcga atggcacgca gcgtctcctc cgccccctcc
     1741 agctgggcgc gcagctccgc gttctcggcg aggagcatcg gctcggttcg ggcttcatca
     1801 ggcatgtcgg ctcaggcgct tcttgccgtc gcacgcagat cgagcccaag cagaatgcgc
     1861 tcggtctgcg acatgtcgcc aatgaaccgg cgcagcggca gcggcagctt cttgattagg
     1921 gtcggcgcgg caatgatctg ctcgccgatg gccagcatcg gatgccggga gatatccacg
     1981 acctccagct cgtaacgtcc ctgcaggtgc tcctcgcaga tcttcctgat gttcaggatt
     2041 gcccgggtgg attgcagcgt ggtgccggta atgtagagcc gcagcatgta gcgagctttg
     2101 gcgcactgag ct
//
LOCUS       DASUMG010000539         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_715505, whole genome shotgun sequence.
ACCESSION   DASUMG010000539 DASUMG010000000
VERSION     DASUMG010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715505"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..517
                     /locus_tag="VFX46_08985"
     CDS             65..517
                     /locus_tag="VFX46_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX5600759.1"
                     /translation="MGDRIRAVTRAEIVRLARTWIGTPYHHQASLAGVGTDCLGLIRG
                     IWRALYGREPVELPAYTRDWSEASGCETLLEAARRHLVEVNKADAQPGDVLVFRLRPT
                     LPAKHVGILATGITMIHAMENAPVAEVALSNWWRRRITAAFSFPEVTG"
     gene            517..>4421
                     /locus_tag="VFX46_08990"
     CDS             517..>4421
                     /locus_tag="VFX46_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase/phage tail family protein"
                     /protein_id="HEX5600760.1"
                     /translation="MATLALAAAGAAAGSVLLPSGLSVFGATIAGATIGSQIGALAGS
                     FVDQALFGVSGQNRTFTGPRLSDLRVTASTEGAPIPRVYGRARVGGQVIWATDFEEEV
                     VTSKVSGGGKGGGSSGGSAKQIEYRYYANFAVGLVEGEISGIGRVWADGQELDLSTVT
                     WRLYTGSEDQLPDSLISAREGAGQAPAYRGLAYIVFERMPLAPFGNRIPQLSFEVFRP
                     VDDFHRLVRGVVLIPGSGEFVYATQEVTRREAGGMQVSENVHTRQGGSDWTVAVDQLQ
                     ASLPNAKSVSLVTSWFGTDLRASHCEIRPGVEITNKETKPLTWRVAGLSRSQAYLVSR
                     HEGRPAYGGTPSDQTVIAAIRDLKERGLSVTLTPFILMDVPANNTLPNPYNGGAPGQP
                     AYPWRGRITLSLAPGQAGSPDKTPAAATEISKFVGTASINDFTISGNDVIYRGPSEWS
                     YRRFILHYAFLAKAAGGVDAFVIGTEMRGLTQVRSSPDTYPFVAALVQLAADVKAVLG
                     PNTKVTYAADWSEYFGHHPQDGSGDVYFHLDPLWASPSIDAIGIDLYWPLSDWRDGYD
                     HADAIAGVQSIYDLNYLKSNIAGGEGYDWYYASAGDRASQIRTPITDGQGKPWIFRYK
                     DLKSWWRNPHYNRPGGIEQSTPTEWVPESKPVWFMEIGCPAIDKGANQPNVFVDPKSS
                     ETVLPYFSNGQRDDFIQRRYLQALIEAFDPEHPGAMPDLNPESSVYEGRMVDPGRIHV
                     YTWDARPFPAFPSDTHTWGDSVNWHRGHWINGRVTSAPVGDLLARLLDDAGFAHYETS
                     ALKGILPGYTIDRVMSPREAIQPLELAYFFDAVESGDKIVFRPRGALAPAIEVSADDL
                     VEERPGSGLLRLTRAQETELPGSAKITYISATNDYRQAVAEARRLAGASGRVSTADLA
                     LVLQDEQAAAIADSWLFEVWAARERASFLLPPSRLAIEPSDTILILNNGRSRLFRVTG
                     IGDRGAREIEALSIDPELYGLGEGRTREKKSNPPAPSGQPIGHFLDLPLLRGDEPEHA
                     GYFAATQFPWPGAVALFRSPTETGFTLQALAGAPATSGEVLDALKPGPVGRYDWAARI
                     RVRLDYGELESAEEIQVLAGTNVAAVRNSAGEWEVLQFRSAKLVAPATYELTGLLRGQ
                     AGTEGAMQVEVPPGAPFVLVTEALARVDMAPGDIGLAFNWRFGPAERDIGHPAYVELQ
                     HTFRGVGLRPLSPVHVRGRRLGTGDLVLSWIRRTRIGGDSWEVNEVPLAEDTERYEVD
                     IFDGETVKRTLIATAPTITYSKGDQIADFGKLPAAITVRV"
BASE COUNT          861 a         1247 c         1371 g          942 t
ORIGIN      
        1 ggggttttcc ccatatgccc ggaaatgatt tcgtaacctc ctatgcacgc aggagggcgg
       61 gttgatggga gatcgaatcc gcgccgtaac tagggcagag atcgttcgcc tggcgaggac
      121 ctggatcggt acaccgtatc atcatcaagc aagtctcgct ggggttggca cggattgcct
      181 gggtctcatt cgaggcatct ggcgcgcact ttacggccgc gagcctgttg aactgccagc
      241 ctacacccgc gattggtcgg aagcgagcgg ctgcgaaact ttgctggaag cagctcggcg
      301 ccaccttgtc gaggttaata aggcagatgc acagcccggt gatgttctgg tcttccgatt
      361 gagaccgaca ttaccagcga agcatgtcgg catactcgca acagggataa cgatgatcca
      421 tgcgatggag aatgcgcctg ttgcggaggt ggcgctttcc aattggtggc gaagacgcat
      481 tactgccgcc ttttcatttc ctgaggtcac gggctaatgg ccactcttgc tcttgctgca
      541 gcgggcgctg ccgcgggcag cgtgctgctg ccgtcaggat tgagcgtgtt cggcgctacc
      601 atcgcaggcg cgacgatcgg ctcgcagatc ggcgcactcg ccggctcttt cgtcgaccaa
      661 gcgctctttg gcgtatccgg acaaaatcgc accttcaccg gcccgcgcct ctccgatttg
      721 cgcgttacgg cttcgacgga gggcgcgcct attcctcgtg tctatggacg ggcccgtgtc
      781 ggtggacaag tgatttgggc gacggacttt gaggaagagg tcgtcacctc gaaagtgtcc
      841 ggtggcggca agggtggtgg aagcagcggc ggctctgcca aacaaattga ataccgctac
      901 tatgcgaatt tcgctgtcgg cttggtggaa ggagaaattt cggggattgg tcgagtctgg
      961 gcagatggac aggagctgga tcttagcact gtaacgtggc ggctctacac cggcagtgag
     1021 gatcagctgc cggacagcct tatcagcgcg cgcgagggcg cggggcaggc gccggcatat
     1081 cgcggcttgg cctatatcgt ctttgaaagg atgcctctcg cgccttttgg caatcgaata
     1141 ccgcagcttt cgttcgaggt ttttcggccg gtcgacgatt ttcatcgatt ggttcggggc
     1201 gtggttctca ttccggggtc cggcgagttc gtttacgcaa cccaggaagt cacccgccgt
     1261 gaggcgggcg ggatgcaggt ttccgaaaac gttcacacgc gccagggcgg tagcgattgg
     1321 accgtcgcgg tagatcagct gcaggcgtca ttgccgaacg caaaatccgt ttctctcgtg
     1381 acgagctggt tcgggacaga tttgcgcgca agccattgtg agattcgtcc gggggtggaa
     1441 atcaccaata aagaaaccaa acctctgaca tggcgggtgg ctggtctctc ccgctcgcag
     1501 gcgtatctcg tcagccggca cgagggcagg cccgcctacg gcggtacacc gtcggatcag
     1561 accgttattg cggccattcg tgatttgaag gagcgcggcc tttccgtgac gctcactccg
     1621 ttcattctga tggacgtgcc ggcaaacaat acgctgccca atccgtacaa tggtggtgca
     1681 ccaggccaac ccgcttatcc ttggcgagga cgcattacgc ttagcctcgc gccgggacag
     1741 gccggtagtc ctgataaaac gcctgctgcc gcgaccgaaa tttccaaatt cgtggggact
     1801 gcctccatca atgatttcac aatttcgggc aacgacgtaa tctatcgggg tccgtcggaa
     1861 tggtcctatc gccgtttcat attgcattac gcttttctgg cgaaagcggc aggtggcgtt
     1921 gacgctttcg tcatcggaac agaaatgcgg ggtctgacgc aggtccgtag ctcacctgat
     1981 acctatcctt tcgtagcggc tctcgttcag ctcgcagcag acgtgaaggc tgttctggga
     2041 ccgaatacca aggtgaccta tgccgccgac tggtcggaat attttggtca tcatccgcag
     2101 gatggctccg gcgacgtcta cttccatctg gatccgctgt gggcctcgcc gtcgattgac
     2161 gccatcggca tcgatctcta ttggccgtta agcgactggc gtgatggcta cgaccacgca
     2221 gatgccatcg ccggtgtgca gtcgatttac gatctcaatt atctgaaatc gaatatcgca
     2281 ggcggtgaag gctacgactg gtactacgca agcgcaggcg atcgggcgag ccaaattcgc
     2341 acgccaataa cagacggtca gggtaagccg tggattttcc gttataagga cctgaaatcc
     2401 tggtggcgta atccgcacta caatcggccc ggaggaatcg agcagtcgac ccccacggaa
     2461 tgggtgccgg agtcaaagcc cgtttggttc atggagattg gttgcccagc catagacaag
     2521 ggcgccaatc agcccaacgt tttcgtcgac ccgaagagct ccgagacggt gctgccgtac
     2581 ttctccaacg gccaacggga tgacttcatc cagcgtcgat acttgcaggc gctgatcgag
     2641 gcattcgatc ctgagcatcc tggcgcaatg ccggacctca atcccgagtc gtcagtgtat
     2701 gaaggaagga tggtcgatcc gggacggatc catgtttata cctgggacgc acgacctttt
     2761 ccggcatttc cttccgatac ccacacgtgg ggtgacagcg tcaactggca ccgggggcat
     2821 tggatcaacg gccgcgtcac cagcgcaccc gtcggtgacc tcctggcacg cctcctcgat
     2881 gatgctgggt ttgcgcacta cgagacgagc gctctcaaag gaatacttcc cgggtacacg
     2941 attgacaggg tgatgtcgcc gcgtgaggcg atccaaccgc tggagctcgc gtacttcttc
     3001 gatgccgtcg aaagtggaga caaaattgta ttccggccac ggggtgcgct tgcgcctgca
     3061 atcgaggtct cggcagacga cttggtcgaa gaacggccgg gaagcggcct tttacggctc
     3121 acacgcgccc aggagacgga gttgcccggc tccgcgaaaa tcacctacat ctcggcaaca
     3181 aatgattatc ggcaggccgt ggccgaagca cgccgcctgg ccggtgccag cggtcgcgtc
     3241 agcacagctg accttgcgct cgttttgcaa gacgagcaag ctgcggcgat cgcggattcc
     3301 tggctttttg aagtatgggc tgcgcgggaa cgcgcgtcgt tcctgctacc gccgagccgg
     3361 ctcgctattg agccgtcgga tactattctg atcttgaaca atgggcgctc tcgattgttt
     3421 cgcgtaaccg gtattggcga tcggggcgcg cgcgaaatcg aagcattgag catcgatcca
     3481 gagctttatg gattgggcga gggccgcaca cgtgagaaga agagcaatcc gcccgcgcct
     3541 tcggggcagc caatcgggca ttttctcgat ctgccattac tacggggcga cgagcccgag
     3601 catgcaggat attttgcggc cactcagttc ccgtggcccg gcgccgttgc gctttttcgc
     3661 tcaccgacag agaccgggtt tacgctccag gccctcgcgg gtgcaccggc gacctcgggc
     3721 gaggttctcg atgctctgaa gccaggcccc gtcggccgct atgattgggc agcgcgaatt
     3781 cgtgttcggc tcgattatgg cgagttggaa tcggcagagg aaatccaggt gctggctggc
     3841 accaatgtag ctgcggttcg caattctgcc ggggagtggg aggtattgca gttccgatcg
     3901 gcaaagctgg ttgcgcccgc gacatacgaa ttgacaggcc tgctgcgcgg tcaagcggga
     3961 accgagggtg caatgcaagt ggaggtgccg ccaggcgcac ctttcgtgct cgttactgag
     4021 gctctcgcgc gggtcgacat ggcgccaggt gatattggat tggctttcaa ctggcgtttc
     4081 ggcccagcgg aacgggatat cgggcatccg gcatacgtag agctgcagca cacattccgc
     4141 ggcgtcggtc ttcgcccact cagtcccgtt cacgttcggg gacgacgatt gggaacaggc
     4201 gatctcgtcc tctcctggat tcgccgaaca cggatcggtg gcgacagctg ggaggtcaac
     4261 gaagtacccc ttgctgagga tacagagcgt tatgaggtgg acatcttcga cggcgaaacg
     4321 gtcaagcgca cgctgatcgc tacggcgcca accattacgt attcgaaggg cgatcaaatt
     4381 gccgattttg gcaagctgcc agccgctatc actgtgcgcg t
//
LOCUS       DASUMG010000540         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_395719, whole genome shotgun sequence.
ACCESSION   DASUMG010000540 DASUMG010000000
VERSION     DASUMG010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395719"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1835
                     /gene="istA"
                     /locus_tag="VFX46_08995"
     CDS             336..1835
                     /gene="istA"
                     /locus_tag="VFX46_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX5600761.1"
                     /translation="MKQVELYARVRYAVQIEGLSERAAARRFGIDPRTVAKMMRFSVP
                     PGYRRMKPPVKPRLGPFIAAIDGILAEDKGRPRKQHHTAKRICERLRDEHGFTGGITI
                     VKDYVAGWRQRAREMFVPLAHPPGHVQVDFGEAIGIIGGVECKIHFFAFDLPHSDACF
                     VVAYPAETKEAFCDGHVKAFAFFGGVPKSILYDNTRIAVARILGDGVRQRTQVFSELQ
                     SHYLFEDRFGRPGKGNDKGKVEGLVGYARRNYMVPVPAFDSFEALNAHLLECCRKRMR
                     ERLRGHAETIGERLERDLAALRRPLPPPYDACEKVPATVSSLSLVRYRRNDYSVPTCF
                     GHRQVMVRGYVHEVVIACGTEIIARHPRSYEREDFVFDPLHYLSLIERKINALDQAAP
                     LAGWQLPEAFATLRRLLEARMGKAGKREFVQALRLMEAFPIEAVTGAVGDAIARGAVG
                     FDAIKHLVLCRIERRPPRLDMSIYPYLPRAMVATTSARSYMALLAGAGS"
     gene            1832..2626
                     /gene="istB"
                     /locus_tag="VFX46_09000"
     CDS             1832..2626
                     /gene="istB"
                     /locus_tag="VFX46_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX5600762.1"
                     /translation="MSDTPQVLLAHHLKTLKLPTFLREYDKVARQCAAEGVDYPRFLL
                     RLAEQELIDRQRRMVERRIKAAKFPTVKSLDSFDFAALPSLNKALVMELARCEYIERH
                     ENVIALGNSGTGKSHIALGLGLAACQKGLSVGFTAAAAIVHELMEARDEKRLLRLQRQ
                     LAAYKLLIVDELGYVPLSQTGAELLFEVFSQRYETGSTIVTSNLPFDEWTSVFGAERL
                     TGALLDRLTHHVHILEMNGDSYRLAHSKRRSRRPRTDATSDAATQD"
     gene            complement(2838..>3119)
                     /locus_tag="VFX46_09005"
     CDS             complement(2838..>3119)
                     /locus_tag="VFX46_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600763.1"
                     /translation="AHEAQDGFGSLTVRLGKSILCPDCPRSLPNTHVEVCFVALPNPS
                     REGLAAPKGSALARLVVELRDQERMGVRWYDTARNDTRPQVYGRSADDR"
BASE COUNT          558 a         1029 c         1017 g          515 t
ORIGIN      
        1 cgagacgcgc cttgagcgcc aggacaaggg cctcgatccg atcgcgccgc agctgagcgg
       61 gaagtacgcc ccagcataga tctcttcacc tgcttcctga cagtaggctg tcaggacacc
      121 ttcgctacat tgatcgtccc agcgacaggg gaactgaacc catggccgac aacgagcagc
      181 atcgcgccaa gctgcaggag ctcaaccgca actacatccg atctgtaagg gccggagcaa
      241 aaatccctca gtgaagcggc cgattttgtt cggttgcgcc ggagtaagaa tccggcagct
      301 ggaagccctg tgccggcgtc ggcagagggc gggggatgaa gcaggtggaa ctctatgcga
      361 gagttcgcta cgcggttcag atcgagggtt tgagcgagcg agcggcggcg cgccggttcg
      421 ggatcgatcc gagaacggtt gccaagatga tgcggttctc ggtaccgccg ggctaccggc
      481 gcatgaagcc gccggtaaag ccgaggctcg ggccgttcat cgccgccatc gacggtatcc
      541 tggccgagga caaaggccgg ccgaggaagc agcatcacac ggccaagcgg atctgcgagc
      601 ggctgcgcga cgagcatggc ttcacgggcg gcatcacaat cgtcaaggac tacgttgccg
      661 gctggcggca gcgggcacgg gagatgttcg tgccgctggc gcatccgccg ggtcatgtgc
      721 aggtcgattt cggcgaggcg atcggcatca tcggcggggt ggaatgcaag atccacttct
      781 tcgcgttcga cctgccgcat tcggatgcct gcttcgtcgt ggcctatccg gccgagacca
      841 aggaggcctt ctgcgacggc cacgtgaagg cgttcgcctt cttcggcggc gtaccgaagt
      901 cgatcctcta cgacaacacc aggatcgcgg tggcccgcat tctgggcgac ggggtacgac
      961 agcgcacgca ggtgttcagc gagctgcaat cgcattacct gttcgaggac cggttcggcc
     1021 gtcctggcaa gggcaacgac aagggcaagg tcgagggact ggtcggctat gcgcgccgga
     1081 actacatggt gccagtgccg gcgtttgaca gcttcgaggc actcaacgcc catctgctcg
     1141 agtgctgcag gaagcgcatg agggagcgcc tgcgtgggca tgccgagacc atcggcgagc
     1201 ggctcgagcg ggatctggcg gctctgcgca ggccgctgcc gccaccctat gacgcctgcg
     1261 agaaggtgcc ggccaccgtc tcctcgctgt cgctggtgcg ctaccggcgc aacgactact
     1321 cggtgccgac gtgctttggc caccggcagg tgatggtgcg cggctacgtg cacgaggtgg
     1381 tgatcgcctg cggcacggag atcatcgcgc gacatccgcg ctcctatgag cgcgaggact
     1441 tcgtgttcga cccgctgcac tacctctcgc tgatcgagag gaagatcaac gctctggacc
     1501 aagccgcacc gttggccggc tggcagctgc cggaggcgtt cgccaccctg cgccggctgc
     1561 tggaggcgcg catgggcaag gcgggcaagc gcgagttcgt gcaggcgctg cggctgatgg
     1621 aggcgttccc gatcgaagcc gtcactggtg ccgtcggtga cgccatcgcc cgtggcgccg
     1681 tcggcttcga cgccatcaag cacctggtgc tgtgccgcat cgagcggcgc ccgccgcgcc
     1741 tggatatgag catctatccc tacttgccca gggcgatggt ggccacgacc tcggcacgct
     1801 cctacatggc cctgctggcg ggagcgggat catgagcgac acaccccagg tcctgctcgc
     1861 ccaccacctg aagacgctga agctgccgac cttcctgcgc gagtacgaca aggtggcgcg
     1921 ccagtgcgcc gccgagggcg tcgactatcc gcgcttcctg ctgcgcctgg ccgagcagga
     1981 gctgatcgac cgccagcggc gcatggtcga gcgtcgcatc aaggcggcga agttcccgac
     2041 ggtgaagagc ctggacagct tcgactttgc ggccctgccg tccctgaaca aggcactggt
     2101 gatggaactc gcccgctgcg agtacatcga gcggcacgag aacgtcatcg cccttggcaa
     2161 ctccggcacc ggcaagagcc acatcgcgct ggggctgggg ctcgccgcct gtcagaaagg
     2221 actgtcggtc ggcttcacgg ccgcggccgc catcgtgcac gagctgatgg aggcgcgcga
     2281 cgaaaagcgg ctcctgcgcc tgcagcgcca gctcgccgcc tacaagctgc tgatcgttga
     2341 cgagctcggc tacgtgccgc tgtcgcagac cggcgccgag cttctgttcg aagtgttctc
     2401 ccagcgctac gagaccggct ccaccatcgt caccagcaac ctgcccttcg acgagtggac
     2461 atccgtgttc ggcgccgagc gcttgaccgg cgcattgctc gaccggttga cccaccacgt
     2521 ccacatcctc gagatgaacg gcgacagcta ccgcctcgct cactccaagc gccgatcacg
     2581 ccggccccga acagacgcga cctccgacgc cgccacccag gactgaggcc gccaaaagca
     2641 agaaggcccc cgatatcggg ggccttcttg ctcgccgtag cgatggattt ttgctccggc
     2701 ccgccgatgc aatttctctc cggcgttgac accgcatctg ccactaccgc gagtgccacc
     2761 cacgcgtggc ctgccgagcg ttcagctgcg gcctgcggca gcatctgaac gacaatctcc
     2821 aggtcgcgac cctgagctca gcgatcatcg gcgctccgcc catagacctg tggccgcgta
     2881 tcgttgcgcg ctgtgtcgta ccagcggaca cccatgcgct cctggtcgcg aagctcgacg
     2941 acgagcctcg caagagctga gcccttcgga gcggcgagcc cctcgcgaga cggatttgga
     3001 agggcaacaa aacacacctc aacgtgggta ttcggcagag atcgggggca gtccggacac
     3061 aatatgctct tgccgagcct taccgttagc gacccaaagc cgtcctgggc ttcgtgagc
//
LOCUS       DASUMG010000541         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_74530, whole genome shotgun sequence.
ACCESSION   DASUMG010000541 DASUMG010000000
VERSION     DASUMG010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74530"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFX46_09010"
     CDS             complement(<1..253)
                     /locus_tag="VFX46_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600764.1"
                     /translation="MVALLPAPLAAGEAVGQHGIAMHGEPALPPDFEHLPYVNPDAPK
                     GGQLRMGVLGTFDSLNPFIIKGVTPSGVREYVYESLMARS"
     gene            600..1361
                     /locus_tag="VFX46_09015"
     CDS             600..1361
                     /locus_tag="VFX46_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion associated locus B family protein"
                     /protein_id="HEX5600765.1"
                     /translation="MANSSWLAISAARAVTGVAVAFGLHLAISGAALAQQGKQAEGQQ
                     QSAWVKLCEKAPLASRDKEGKLVREEKEICLTHHERLDGNTGMVIVSAALRHVEGIDK
                     EHLMIMVPLGMAIPPGVRAAVYTPEQWEKAQKNEKIDEDSLKPISLNYTLCHPAGCTA
                     EVEATKELVDSMKSGGGLMVMAINANGQPIAFPVPLDGFTAAHSGPPVDNEAYAKARS
                     QLMEQIRERRAEMIKQAQEKQKQAAEKQQPAAKKK"
     gene            complement(1515..2576)
                     /locus_tag="VFX46_09020"
     CDS             complement(1515..2576)
                     /locus_tag="VFX46_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HEX5600766.1"
                     /translation="MTKCTKPLTTPADLAAAGFIPDEALERLEAVAARYAVSITPAMA
                     ELIDPADADDPIARQFVPDSRELDMAPSEIADPIGDHSHSPVPGVVHRYPDRVLLKVV
                     GVCAVYCRFCFRREMVGPEQGAPLSADQFSAALDYIRGRPEVWEVILTGGDPFVLSPR
                     RIAEITTALDTIPHVAVLRWHTRVPVADPARISDELVSALRNTGKAVWVAVHANHPRE
                     LTQAAKTACAKLVDAGIPLVSQSVLLKGVNDDPETLSELMRAFVACRIKPYYLHHGDL
                     APGTSHLRTRIADGQQLMRQMRGRVSGLCQPTYVLDIPGGAGKVPIGPCHAHPGKEPG
                     SYELEDPDGHRHTYRDSVR"
     gene            complement(2728..>2971)
                     /locus_tag="VFX46_09025"
     CDS             complement(2728..>2971)
                     /locus_tag="VFX46_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX5600767.1"
                     /translation="ELANGFGELTDPHEQRRRFEAEMAEKARVYGESYPLDEEFLAAL
                     HHMPPASGIALGFDRLVMLATGAERIEQVIWTPLPE"
BASE COUNT          600 a          904 c          944 g          523 t
ORIGIN      
        1 gcgagcgtgc catcaggctt tcatagacgt actcacgaac acccgatggc gtcacgccct
       61 tgatgatgaa tgggttcagg ctgtcgaacg tgccgagcac gcccatacgt agctgaccgc
      121 ccttgggcgc atcgggattg acgtagggca ggtgttcgaa atcgggcggc agcgccggct
      181 cgccatgcat cgcgatgccg tgctgcccga cggcctctcc ggctgcaagt ggcgccggaa
      241 gcagcgcgac catgagcatg gacgcgaggg ttctcagcag gatcactggc cttccgcttg
      301 ctgaaccaaa atctgacggc tcgacgagat gccaccgccg ttcgagtgca gcccattacc
      361 acaattgggg cgcattacga ccccatgctc actgcgacgt tgcgactttg ccacgtcatc
      421 ggcgcgggct cacccatgca caggccacgc aggcaggcgg tcacgtgatt gccgcattcg
      481 caccattagt ccgcgcaagc acacgtcgca atgggggcgt gtcaacccgg cgcgccgtga
      541 gtgccggggg tatggtggga cgggcaggcc gtaccgctca cagcgaacgg gaacacacga
      601 tggcgaattc aagttggctc gcaattagcg ccgctcgcgc cgtaacaggt gtggcggtgg
      661 cattcggtct tcatttggcg atttctggcg ctgccttggc ccagcagggc aagcaagctg
      721 aaggtcagca acagagcgcg tgggtcaagc tctgcgagaa ggctccgctg gcgagccgcg
      781 acaaggaagg gaagctcgtc cgggaggaaa aggagatctg cctcactcac catgagcggc
      841 tcgatggcaa tacggggatg gtcattgtgt cggcagccct ccgccacgtc gaagggatcg
      901 ataaggagca cctgatgatc atggtgcctc tgggcatggc cattccgccc ggcgtcaggg
      961 ctgccgtcta caccccggag cagtgggaaa aggcccaaaa gaacgagaaa atcgacgagg
     1021 attcacttaa gccgatcagc ctcaactata ccctgtgcca tccggcgggt tgcacggcag
     1081 aggtggaggc caccaaagag ctcgtggact cgatgaaatc gggcggtggt ttgatggtca
     1141 tggcgatcaa cgccaacggc caacccatcg catttcccgt tcctctcgat ggctttacag
     1201 ccgcgcattc cggcccgccg gtcgacaacg aagcctacgc caaggcccgt agtcagctga
     1261 tggagcagat cagggagcgc agggccgaga tgatcaaaca ggcccaggag aagcagaaac
     1321 aggctgccga gaagcaacag cccgccgcga aaaagaaata aaggcagagg ctatttcatg
     1381 aaagccgctg tggggtggca ttgctggcca ccccagcgta ttttggcccc cttcgaagcc
     1441 gcttgatctt gcgagccgcg ctggcgttcg tcaggagtca gcggcggtgg gctcaatttt
     1501 cgaaatcaat cagcttacct gacgctatcg cgatacgtgt ggcggtggcc gtccgggtct
     1561 tccagctcat agctgccagg ttccttgcca gggtgtgcgt gacacggccc aatcggcact
     1621 ttgccagcgc cgcccgggat atcgagcacg tatgtcggct ggcacaatcc cgaaacgcgt
     1681 ccgcgcatct gccgcatgag ctgctggcca tcggcgatac gggtgcgcag atgagacgtc
     1741 ccgggtgcca gatcgccgtg atgcaggtag tacggcttga tgcggcatgc gacgaacgcg
     1801 cgcatgagct cggaaagcgt ttccggatcg tcattcacgc ccttgagcag caccgactgg
     1861 ctcaccagag gaatgcctgc gtcgaccaac ttcgcacatg cagtcttcgc ggcctgcgtc
     1921 aactcgcggg gatggttggc gtgcaccgcc acccacacgg ctttgcccgt gttacggaga
     1981 gccgacacca actcatcgga aatccgcgct ggatccgcga cgggcacgcg cgtgtgccag
     2041 cgcaacaccg cgacatgcgg aatggtgtcc agcgccgtag tgatctccgc gatccgtcgc
     2101 ggcgaaagaa caaagggatc gccgcccgtc aagatgacct cccagacctc tggccggccg
     2161 cggatgtaat ccagtgccgc agagaattga tcagctgaaa gcggtgcgcc ctgctcaggg
     2221 ccgaccattt cccggcggaa gcagaaccgg cagtagaccg cgcagacacc tacgaccttg
     2281 agcagcacgc ggtcggggta acgatgcacg acacccggta cgggggaatg actgtggtca
     2341 ccaatgggat cggcgatctc gctcggcgcc atatccagct cgcggctatc gggcacgaac
     2401 tgccgcgcga tagggtcgtc cgcatcagct ggatcaatca gctcggccat cgccggggtg
     2461 atcgagacgg catagcgtgc cgcgacggcc tcaagccgct caagcgcctc atcgggtatg
     2521 aagccggctg ctgccaggtc ggccggtgtg gtcaagggtt tggtgcattt ggtcatgagg
     2581 cctaggcgcc caattcgacg ccggtgtcaa tgtggccagt aaccccgcgt cgcttccatc
     2641 aactcttgtc gggaagggag gtccaggggc gaggatcgaa gatgctggga cccgcgatcg
     2701 catcgcccgt cggcgtctaa gatgaagcta ttccggcagg ggcgtccata tcacctgctc
     2761 gatgcgttca gcgcctgttg ccaacatcac gagccggtcg aagccaaggg caatgcctga
     2821 agccggcggc atgtggtgga gcgcggccag gaattcctcg tccaatggat agctctcgcc
     2881 ataaactcga gctttttcgg ccatttccgc ttcgaaacga cgccgttgct cgtgtggatc
     2941 ggtcagctct ccgaagccgt tcgcgagctc g
//
LOCUS       DASUMG010000542         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_74643, whole genome shotgun sequence.
ACCESSION   DASUMG010000542 DASUMG010000000
VERSION     DASUMG010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74643"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..367
                     /locus_tag="VFX46_09030"
     CDS             95..367
                     /locus_tag="VFX46_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600768.1"
                     /translation="MSTEIALLEQAWLEAETAADALKLEASKASAELARMRQSAGANG
                     TDLSTLVATVEQLKARQEEAERAASAAFDRYWTAQGNGKDSGSAYA"
     gene            423..1175
                     /gene="sufC"
                     /locus_tag="VFX46_09035"
     CDS             423..1175
                     /gene="sufC"
                     /locus_tag="VFX46_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012092132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX5600769.1"
                     /translation="MLEIRNLHVKIADEDTMILKGVDLVLPKGEVHAIMGPNGSGKST
                     LAYVLAGREDYEVTEGEILWNGENLLEMEPDERAAKGVFLAFQYPIEIPGVAGLTFLR
                     TALNALRKKREEPELTTPEFMKLVRERAGKLNIDIEMLKRPVNVGFSGGEKKRSEILQ
                     MALLEPTLCVLDETDSGLDIDALRIVADGVNALRSPDRAMLVITHYQRLLNYIVPDKV
                     HVLADGHIQRTGDKELAVELEQSGYAEFTKAA"
     gene            1196..2518
                     /gene="sufD"
                     /locus_tag="VFX46_09040"
     CDS             1196..2518
                     /gene="sufD"
                     /locus_tag="VFX46_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX5600770.1"
                     /translation="MNVGLTKTKAEQAFTESFEAIASKLPGSEAVRELRRKAIGTFAA
                     LGLPHRRIEEWKYTDLRNLLKEAFTPAIGKAASPSEADLNAALGPLAALDTYRIVLVD
                     GAFNASLSTAQRPDGLSVKPLSEALAESPDIVRMKRDQDAIEALNSAFATDGAVVSVA
                     DGTELDKPLLLVFARAGADRKTITTRNVVNIGKGAKVTLVEAFVPLAGSADEDQHNSA
                     TEIVVGEGAAVTHVKAIVENGKAVHLGSATAEIGADASYRAFQLTAGPGLARNQIFVK
                     FTGEGAKLDCSGSFLATNNEHIDTTLVVDHAVPHCESRELYKGVLDGHGRGIFQGKVI
                     VRPGAQKTDGKQMAQALMLSPDAEFDSKPELEIYADDVICGHGSTSAEIDPEHIFYCR
                     SRGIPEPEARALLIEAFVGEAMEKVEDEPVREALMETARVWLNQRTAR"
     gene            2532..>3561
                     /locus_tag="VFX46_09045"
     CDS             2532..>3561
                     /locus_tag="VFX46_09045"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX5600771.1"
                     /translation="MTQAAAFEAGRRFEVERYRADFPILFREVYGKPLVYLDSAASAQ
                     KPLSVIEAMDHALRFEYANVHRGIHLLSNTATQKYEEARETVRRFLNAPSAEESVFTK
                     NATESINLVAYSYGLANIQEGDEIVLSIMEHHSNIVPWNFLRERKGAVLKWVPVSDNG
                     EFLLDEFERLLGPRTKMIALTHVSNVLGTVVPIKEVCRIARERGIPVLIDGSQGAVHL
                     PVDVQDIGCDFYAFTGHKVYGPSGIGVLWGRKDLLESLPPFLGGGSMIESVTVDGITY
                     SPPPTRFEAGTPPIVEAIGLAAALNYMMQIGRDEIAAYEHDLVTYAHERLSEFNWLRI
                     FGTARDKSA"
BASE COUNT          707 a         1031 c         1209 g          614 t
ORIGIN      
        1 ccggctcaaa gacgtggggc acgggcaaga gggtagagtc ggggccataa ggacgagagc
       61 cagggcttcg gaactaggtg gaggaaacga acggatgtcg accgaaattg ccctgctcga
      121 gcaggcttgg cttgaggccg aaactgcagc cgatgccctg aagctcgaag cttcgaaagc
      181 gagtgcggag ctggcacgaa tgcggcagtc cgcgggagca aacggaacgg atttgagcac
      241 gctcgtcgcg acggtcgagc agctcaaggc gcgccaggag gaagcggaac gtgcggccag
      301 cgccgcgttc gaccgctact ggacggcgca agggaatgga aaggattccg gctccgcata
      361 cgcgtgagcc gggcaggtgt tggaaatggg cgccagaagg cgcctgaagg agagacgtgg
      421 agatgcttga gatccggaat ttgcacgtaa agatcgctga tgaggacacc atgatcctca
      481 agggggtgga ccttgtgctc cccaagggcg aggtgcacgc gatcatgggg cccaacggat
      541 cgggcaaatc cacgctcgcc tatgttctcg ccggccgcga ggattacgag gtgaccgagg
      601 gcgagatcct ctggaacggt gagaacctgc tcgaaatgga gcccgacgag cgggccgcga
      661 agggcgtgtt cctcgccttt cagtatccga tcgagattcc cggtgtcgcc ggcctgacct
      721 ttttgcgcac ggcgctcaac gcgctgcgca agaagcgtga agaaccagag ctcaccacgc
      781 cggagttcat gaagctcgtg cgcgagcgcg ccggcaagct caacatcgac atcgaaatgc
      841 tgaagcgccc tgtgaacgtg ggcttctccg gcggcgagaa gaagcgctcc gagatcctgc
      901 agatggcgct gctcgagccg acgctctgcg tgctggacga gaccgattcc ggcctcgata
      961 tcgatgcgct gcgcatcgtg gcggatggcg tcaacgcgtt gcgcagcccc gatcgtgcga
     1021 tgctggtcat cacgcactat cagcggctgc tgaactacat cgttcccgac aaggtgcacg
     1081 tcctcgctga cggccatatc cagcgcacgg gcgacaagga gctggcggtc gagctcgagc
     1141 agtcgggcta cgccgagttc acgaaggcgg cctgaagcga gcgggtgatt tcgagatgaa
     1201 cgtgggcttg accaagacga aggccgaaca ggccttcacc gagagcttcg aggcgattgc
     1261 ctccaagctt ccgggaagcg aggctgtgcg cgagttgcgc cggaaggcca ttggcacgtt
     1321 tgcggcgctc ggcttgccgc acaggcggat agaggagtgg aagtacaccg accttcgcaa
     1381 tttgttgaag gaggccttca ctccggcaat cggaaaggcc gcatcgccga gcgaggcaga
     1441 cctcaatgcg gcgctgggtc cgttggcggc cctcgacacc taccgcatcg tgctcgtcga
     1501 cggggcgttc aatgccagcc tttccaccgc tcagcgccct gacggattgt cggtgaagcc
     1561 gctgagcgaa gcgctggccg agtcgccgga tatcgtgcgg atgaagcgcg atcaggatgc
     1621 gatcgaggct ttgaacagcg ccttcgcgac ggacggcgcg gtggtgagcg tcgctgacgg
     1681 cacggagctc gacaagccgc tgctgctggt gtttgcccgt gcgggcgctg accgcaagac
     1741 gatcacgacc cgcaacgtcg tcaacatcgg caaaggtgcg aaggtgacgc tcgtcgaagc
     1801 cttcgtgcct ctggcgggat cggcggacga ggaccagcac aactctgcga ccgagatcgt
     1861 ggtcggcgag ggtgctgccg tgacacacgt caaagcgatc gtggagaacg gtaaggccgt
     1921 gcacctcggc agtgccactg cggaaatcgg ggctgacgcc agctaccgcg cctttcagct
     1981 cactgcgggg ccgggcctcg cgcggaacca gatcttcgtc aaattcaccg gcgaaggagc
     2041 caagctcgac tgttccggct ctttcctggc gacgaacaat gagcacatcg acaccacgtt
     2101 ggtcgtcgac cacgcggtgc cgcattgcga gagccgggag ctttacaagg gtgtgcttga
     2161 tggtcacggc cgcggcatct tccaaggcaa ggtgatcgtt cggccgggcg cgcagaagac
     2221 ggacggcaag cagatggctc aggcgctgat gctgtcgccg gatgcggagt tcgacagcaa
     2281 gcccgagctt gagatctacg cggacgacgt catctgcggt cacggctcga cgtcggccga
     2341 gatcgacccc gagcacatct tctactgcag atcgcgcggc attccggagc cggaggcgcg
     2401 ggcgctgctc attgaagctt tcgtcggcga ggcgatggaa aaggttgagg atgagcctgt
     2461 gcgcgaggcg ttgatggaaa cggctcgcgt gtggctgaac cagaggacgg ctcgctagag
     2521 ccgattggca cgtgacacag gcagcggcat tcgaagccgg caggcggttt gaagtcgagc
     2581 gctaccgcgc cgacttcccg atcctgttcc gcgaggtcta cggcaagccg ctcgtatacc
     2641 tcgacagcgc ggcatcggcg cagaagccgc tgtcggtcat cgaggcgatg gatcacgcgt
     2701 tgcggttcga atatgcgaac gtgcatcgcg gtatccattt gctctccaat acggcgacgc
     2761 agaagtacga ggaagcgcgc gagacggtgc ggcgcttcct caatgcgcct tcagccgagg
     2821 agagcgtctt tacgaagaac gccacggagt cgatcaacct cgttgcgtat tcgtacgggc
     2881 tagcgaacat ccaggaaggc gacgagatcg tgctctccat catggagcac cactcgaaca
     2941 tcgtcccctg gaacttcctg cgcgagcgca aaggggccgt cctgaagtgg gtgccggtgt
     3001 cggacaatgg cgagttcctg ctcgatgagt tcgagcggct gctcggaccc cgcaccaaga
     3061 tgatcgcgct gacgcacgtt tcgaacgtgc ttggaacggt cgttccaata aaggaggttt
     3121 gccgtattgc gcgcgagcgc ggcattccgg tgctgattga cggctcgcaa ggggcggttc
     3181 atctccccgt cgatgtccag gacattggct gcgacttcta cgcgttcacc ggccacaagg
     3241 tttatgggcc gtccggtatc ggggtgctct ggggccgcaa ggatctgttg gagtcgttgc
     3301 cgccgttctt gggcggtggc tccatgatcg agagcgtgac cgtcgatggc atcacgtata
     3361 gcccgccgcc cacgaggttc gaggctggaa cgccgccgat cgtcgaggct atcggcttag
     3421 ctgcggcgct taactacatg atgcagatcg ggcgcgacga gatcgccgcc tacgaacatg
     3481 acctcgtcac gtacgcgcac gagcggttga gcgaattcaa ttggcttagg attttcggga
     3541 ccgcccggga caagagcgcc a
//
LOCUS       DASUMG010000543         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1199142, whole genome shotgun sequence.
ACCESSION   DASUMG010000543 DASUMG010000000
VERSION     DASUMG010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199142"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VFX46_09050"
     CDS             complement(<1..566)
                     /locus_tag="VFX46_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX5600772.1"
                     /translation="MPSIPTVAIIGRPNVGKSTLFNRLTGRRAALVSEMPGLTRDRRE
                     GIATLGRHEIRLVDTAGLEEAPPGSIAARMTRQTEQALKDADLVLFVIDAREGVTPAD
                     AAFARLVRTAGRPAILVANKCEGRKGFDGFYEAFELGLGEPIAISAEHGEGTGELVAD
                     IIRALGLEDDEARRVAEEGEEGDATAARP"
     gene            complement(630..1982)
                     /locus_tag="VFX46_09055"
     CDS             complement(630..1982)
                     /locus_tag="VFX46_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX5600773.1"
                     /translation="MGEVGKRSQRLGQVLAPAILVAIGLGGCGGSLPSLPKISELNPF
                     AEKEQPLPGKRIPVMQARDSLGAELAAADKPVVLPPPRANENWTQPGGEPNNSPGHLE
                     LNSSLRRAWSADVGTGSSSKGKLTASPVVYGGRVFTLDATARVTALSASSGSAIWRVS
                     LVPENEREEGGFGGGLAIENGRLYAATGFGTVVALDSATGKVLWTKNIGVPVRASPTA
                     VDDKVFVVTTEGHVYALAGYDGGELWTFRGIPERTSIISNPSPAVEGGVVVVPYPSGE
                     LVALRADDGTPVWTESLARTRTVSSLASLSDAARPAIAGGVVYAVGHGGRLIATEIKS
                     GERLWSLNVAGTQAPVVAGDNVFVVDTSGQLHAITRRDGKVIWTAKLPGTSTWSGPTL
                     AGNHLWLTSNKGQLVGVDPATGRVTAQQNLDSPIYIAPVVAGGRMYVLTDKARLVAFN
                     "
     gene            complement(1995..2726)
                     /locus_tag="VFX46_09060"
     CDS             complement(1995..2726)
                     /locus_tag="VFX46_09060"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX5600774.1"
                     /translation="MADDRDPILRELESEVRREQLAKLWDRYGIYALAVAVLIIVGVG
                     GYKWWEARTLAAAAEAGARYQAAMQLAEQGKDAEARQAFEAMVRDAPSGYATLARLQL
                     AGEAASAGRTDDAIAAYEGIAADGSVDELLRQYAQLQIAMLKVDTADFTEMQNRLTPL
                     MSEGNAWRHNARELLGLSAFRAGRLEEARQIFLELAADQKTPPSVRERAGLIMGLITS
                     AEAQKTAAAAQPASGTPPPEAPKVQ"
     gene            complement(2928..>3155)
                     /locus_tag="VFX46_09065"
     CDS             complement(2928..>3155)
                     /locus_tag="VFX46_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HEX5600775.1"
                     /translation="NGDLASILLFGSFLAYAIYDRISVKQRMVAPKPGPSSGGIGNDI
                     IVVAVGVALYAFMLFVGHAWLIGVPLLPGWY"
BASE COUNT          567 a         1076 c          945 g          567 t
ORIGIN      
        1 ggtctggcgg cggtcgcgtc gccctcctcc ccttcttccg ccacccgccg ggcctcgtca
       61 tcctcaagac cgagagcccg tatgatgtct gccaccagct cgcccgtgcc ttctccatgc
      121 tcggcggaaa tcgcgatggg ctcgccgagg ccgagctcaa atgcctcgta gaagccgtcg
      181 aagcctttgc ggccctcgca cttgttggcg accagaatgg cgggacgccc agcggtgcgg
      241 accagccgcg cgaatgcggc atcggcgggc gtcaccccct cccgggcatc aattacgaac
      301 agcaccagat cggcatcctt cagcgcctgc tcagtctgcc gggtcatgcg ggcggcgatg
      361 ctgccaggtg gagcctcctc gagcccagcc gtgtcgacga gacggatctc atggcgcccc
      421 aaagtcgcga tgccttcacg gcggtcgcgc gtcaggcccg gcatttccga gacgagcgcg
      481 gctcgtcggc cagtaagccg gttgaacaat gtcgatttac cgacgttggg tcggccgatg
      541 atggcaactg taggaatgga cggcatcggg agcgcacgag aactcggctt cgcaagcggc
      601 ctgacccgtc agcgcctgcg aagattgcgt cagttgaagg cgaccagacg tgccttgtcg
      661 gtcagcacgt acatacgccc accggcgacg accggcgcga tatagatggg cgaatcgaga
      721 ttctgctgcg ctgtcacacg cccggtcgca gggtcaacgc caaccagctg gcctttgttc
      781 gatgtgagcc aaaggtgatt gccggcaagg gtcggccctg accaggtgct cgtacccggc
      841 agcttggctg tccagatcac cttaccgtcg cggcgcgtaa tcgcgtgcag ttgccccgag
      901 gtgtcgacca cgaaaacatt atcgcccgcg actactggcg cctgcgtgcc cgccacattg
      961 agcgaccaaa gccgctcgcc cgacttgatc tccgtggcaa tcagccggcc tccgtgcccg
     1021 acagcataga cgactccacc ggcaatcgct ggccgcgccg cgtcactcag cgaggcgaga
     1081 gatgaaactg tgcgagtgcg tgccaggctt tcggtccata caggcgtgcc gtcgtcggcg
     1141 cgcaaggcaa ccagctctcc agagggataa ggcacgacca cgacaccgcc ttccaccgcg
     1201 gggctcggat tggagatgat gctggtacgt tccggaatac cacggaaggt ccacagctcg
     1261 ccgccatcgt atccagccag cgcgtataca tgcccctcag tggtgacgac gaacaccttg
     1321 tcatcgactg cagtaggcga ggcccgcacg ggcacgccga tgttcttcgt ccaaagcacc
     1381 ttgccggtcg ccgagtcgag ggccacaacc gtgccgaatc ctgtggcggc gtagagccgg
     1441 ccattttcga tggcgaggcc gccaccgaag ccaccctcct cgcgctcatt ttccggtacg
     1501 agtgagacgc gccagatagc tgagccgctc gacgcagaca acgcagtcac ccgagccgtg
     1561 gcatcgaggg tgaaaacccg tcctccgtag actacggggc tggccgtcaa cttgcctttc
     1621 gagctggagc cagtgcccac gtccgcactc caggcccgcc gcaaggagct gttgagctca
     1681 agatggccgg gagagttgtt gggctcgccg cccggctgag tccagttctc gtttgcccgc
     1741 ggcggtggca gcaccaccgg cttatcagcc gctgccagct ccgctccgag gctgtctcgt
     1801 gcctgcatga ccggaattcg cttgccgggt agcggctgct ctttctcagc gaatgggttt
     1861 agctcactga tcttgggcag actcggcaag ctgcccccac aaccgcccag gccgatcgcc
     1921 accagtatgg caggcgctaa tacctgaccc agccgttgcg accgcttccc aacctcgccc
     1981 atcaaatctc cccgtcattg caccttgggc gcttccgggg gcggcgtgcc gcttgccggc
     2041 tgcgcggctg ctgccgtctt ctgggcctcc gcagacgtga tcaagcccat aatgaggccg
     2101 gcgcgctcac gaaccgacgg tggggtcttc tggtctgcgg ctagctccag aaaaatttgc
     2161 ctcgcctcct ccaaacgccc ggcgcgaaaa gccgataagc ccagcagctc gcgcgcattg
     2221 tgcctccagg cgttgccctc cgacatcaaa ggggtgagcc gattctgcat ttcggtaaag
     2281 tcggctgtat cgactttcaa cattgcaatt tgcaactggg catactggcg gaggagctcg
     2341 tcgacgctgc cgtccgccgc gatcccctcg taagcggcga tcgcatcgtc agttctgccg
     2401 gccgaggccg cctcgcccgc gagctgtaga cgggccaacg tggcatagcc ggaaggtgcg
     2461 tcccgcacca tcgcctcgaa ggcctgcctc gcttcggcgt ccttgccctg ttctgcgagt
     2521 tgcatcgccg cctgatagcg tgcgccggcc tcagctgcag cggccaaggt gcgggcctcc
     2581 caccacttgt atccgccgac gccgacaatg atcagaactg cgaccgcgag ggcgtagatg
     2641 ccataccggt cccagagctt cgccaattgc tcgcggcgca cctcgctctc gagctcgcgc
     2701 aaaattgggt cgcggtcgtc agccattctc ggttcggccc tcctgatgcc aaatctctgg
     2761 atacgaacgc gtacccaggc gccgtagaca tagcgcagcg cacattccgc gccaacgcga
     2821 cggcttgccg gaagcggccg ctcaagcgaa actttttaac ttccgcccct tgctgctatt
     2881 tctttccggc ggatttgcgg ccttgctgga gcaacaatgg tcaataatca ataccagccg
     2941 gggagcagag gcactccgat cagccaggca tggccgacga acagcatgaa ggcgtacagc
     3001 gccacgccca ccgcgacgac gatgatatcg ttcccaatcc caccgctgct tggccccggc
     3061 ttcggcgcga ccatcctttg cttcacggat atccggtcgt agatcgcgta ggcgaggaag
     3121 ctgccgaaaa gcaaaatcga ggcgaggtcg ccgtt
//
LOCUS       DASUMG010000544         3158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1199338, whole genome shotgun sequence.
ACCESSION   DASUMG010000544 DASUMG010000000
VERSION     DASUMG010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199338"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VFX46_09070"
     CDS             <1..373
                     /locus_tag="VFX46_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX5600776.1"
                     /translation="GSVSHMFDRIGEVVYGAEVASADTMLEAAIEAGAEDVQSDENGH
                     VITCAFEDLGSVANALEERFGQPTSVKIVWRPNISTQIDEDHAQTVMKLISALEDDDD
                     VQSVFSNFEVSDEVMAKLTAA"
     gene            440..967
                     /gene="ruvC"
                     /locus_tag="VFX46_09075"
     CDS             440..967
                     /gene="ruvC"
                     /locus_tag="VFX46_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX5600777.1"
                     /translation="MRGKAIRIIGIDPGLRRTGWGIIESDGVRLGYVASGAIQSDSDE
                     DLAYRLRELFEGLSSVIATFEPQEAAIEETFVNKDARATLKLGQARGMAMLAPAMRGL
                     RVAEYPPNLVKKSVTGNGHCEKRQIQAMVGVLLPRAKFDSADEADALAIAICHAHHRS
                     GPHERAARLARGLTV"
     gene            964..1593
                     /gene="ruvA"
                     /locus_tag="VFX46_09080"
     CDS             964..1593
                     /gene="ruvA"
                     /locus_tag="VFX46_09080"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002964792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX5600778.1"
                     /translation="MIGRLRGKVDAIGESYLIVDVNGVGYEVQASARTLRSLELGQEV
                     TLSIETHVREDAIRLFGFTAELERAWFRMLQSVQGVGAKVALGILGTLSAQDLINAIA
                     LGNWAAVEKAPGVGRKLAQRIVSELKEKAPQLSMGIRAAPAGVRANGASAASLPQESH
                     VSAEAISALTNLGYPPQQAAAAIAAAIGELGEAADLAQLIKRGLRELAR"
     gene            1616..2044
                     /locus_tag="VFX46_09085"
     CDS             1616..2044
                     /locus_tag="VFX46_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600779.1"
                     /translation="MSNAQRALWTFLFYTLVGPFIGALLISVAIPLALVFGFLPDLGA
                     LETGQISFTGWAALYAYVWGAPAAALAALGLLPFVFRGGTFSWILAAVAGVIAFGVTS
                     IFAPLPVPGTAPYLAFLAGVVSFVCWAVLSKLGVLTGADQ"
     gene            2201..>3158
                     /gene="ruvB"
                     /locus_tag="VFX46_09090"
     CDS             2201..>3158
                     /gene="ruvB"
                     /locus_tag="VFX46_09090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX5600780.1"
                     /translation="MTDRLVTGAKRETDAFEATIRPQSLDEFIGQEQARANLRVFIES
                     ARARGDVLDHVLLAGPPGLGKTTLAQILAKELGVGFRATSGPVLSKAGDLAALLTNLE
                     ERDVLFIDEIHRLNPAVEEILYPAMEDFQLDLIIGEGPAARSVRIDLPKFTLVGATTR
                     AGLLTTPLRDRFGIPIRLNFYTVEELEQVVLRGARVLGAKINAEGAREIARRSRGTPR
                     IAGRLLRRVWDFAVVSKAKVIDAAVADKALRALEVDSAGLDALDHRYLTCIATNYEGG
                     PVGIETIAAALSEPRDALEEIVEPYLLQQGFIGRTPRGRIVTL"
BASE COUNT          563 a          966 c         1038 g          591 t
ORIGIN      
        1 cggctccgtt tcgcacatgt tcgaccgcat cggtgaggtt gtttacggag cggaggtcgc
       61 ctcggcggat accatgttgg aggccgcgat cgaggctggc gccgaggacg tccagtccga
      121 cgagaacggc catgttatca cgtgtgcctt cgaagacctg gggagcgtgg cgaacgcctt
      181 ggaggagagg ttcggtcagc ccacgtcggt caagatcgtc tggcggccca atatcagcac
      241 tcagatcgac gaagatcacg ctcagacggt catgaagctc atctccgccc tcgaggacga
      301 tgatgatgtg caaagcgtct tctccaactt cgaggtgagc gacgaggtga tggcgaagct
      361 cacagcagcc tgacagctgg actcggattc acaactaagc cgacgcctat ctgggaccga
      421 actgtgctaa gcacccccta tgcgggggaa agcgattcgc atcatcggca tcgatccggg
      481 gctgaggcgc actggttggg gcatcatcga gtccgacggc gtaaggctcg gctatgtggc
      541 ctcgggcgca atccagtcgg atagcgacga ggatttggcc tatcgcctcc gtgagctctt
      601 cgaggggctg tcgagtgtaa ttgcaacctt cgagccccag gaagccgcca tcgaggagac
      661 gttcgtcaac aaggacgcgc gggcgacgct gaagctcggc caggcgcgcg gcatggccat
      721 gttggccccg gcgatgaggg gattgcgagt ggccgagtat ccgcccaacc tcgtcaagaa
      781 gtcggttacc gggaacgggc actgcgagaa gcgtcaaatc caggccatgg tcggggttct
      841 cctgcccagg gccaaatttg acagcgccga cgaagccgat gcgctcgcca tcgcgatctg
      901 tcatgctcat cacagaagcg gaccccacga acgtgccgcg cgactggcta ggggattgac
      961 ggtatgatcg gtcgtctgcg ggggaaggtc gatgccatcg gcgagagcta cctgatcgtc
     1021 gatgtgaacg gcgttggcta cgaggtgcag gcgtcggcac gcacgctaag gagcctagag
     1081 ctgggccagg aggttactct gtcgatcgag acccacgtgc gcgaggatgc catacgcctg
     1141 ttcggtttca cggccgagct ggagcgggca tggttccgta tgttgcagtc cgtgcaaggc
     1201 gttggggcga aagtggcgct cggcatactc ggcacgctgt ccgcgcaaga tctcatcaac
     1261 gccatcgcgc tggggaactg ggcggccgtg gagaaagctc ccggcgtggg cagaaagctc
     1321 gcgcagcgca ttgtcagcga gttgaaggaa aaagctccac agctgtccat gggcattcgc
     1381 gcagcgcctg ccggtgtgag ggccaatggt gccagcgccg cgtcactgcc gcaggagagc
     1441 catgtctcgg cggaggcgat ctcggccctg accaacctgg gctatccgcc tcagcaggcc
     1501 gcggccgcta tcgccgcagc cattggcgaa cttggggaag cggcagatct cgcccagctg
     1561 atcaagcggg gcctgcggga gctggcgcga tgacggaccg ggtgtcatgg atggcatgag
     1621 caacgcgcag cgcgcactct ggacctttct cttctacacg ctcgtcgggc ctttcatcgg
     1681 cgcgctgctc atcagcgtcg cgataccgtt ggcgctggtg ttcggattcc tgcctgatct
     1741 tggcgctctg gaaaccggac aaatctcctt cacgggctgg gcagccctct acgcgtacgt
     1801 ctggggtgct cccgctgccg cgctcgccgc gcttgggctc ctgccgttcg tgtttcgcgg
     1861 aggtacgttc agctggatcc ttgcggcggt tgctggggtc attgcgttcg gggtcacctc
     1921 aatcttcgcg ccattgccgg tccctggcac cgctccttac ctcgctttcc tcgcaggagt
     1981 cgtttcgttc gtctgttggg ccgttctctc caagctgggc gtgcttacgg gagccgatca
     2041 gtagtccgtc gcctctgaag cagctccgtg gcgtgagcta ggatgtgtgc cgccgggtct
     2101 gcatgaccaa caacatcaac agcctgccgg gcgacgtcct gcgaattgtg gtagaggttc
     2161 agtcgagcac gaatcacgaa caggagcggg ggcaggtgca atgacggacc gcctcgttac
     2221 aggcgcgaag cgcgaaacgg acgccttcga ggcgacgatc cgcccgcaat ctctggatga
     2281 gttcatcggc caggagcagg cccgcgcgaa cctgagagtc ttcatcgaat cggcgcgggc
     2341 acgcggcgat gtgctcgatc acgttctgtt ggccgggccg cccgggctgg gcaaaacaac
     2401 gctggcgcag attctcgcga aggagctcgg cgtcggtttt cgcgccacct ccgggcccgt
     2461 cctttccaag gctggcgatc tggccgctct gctgaccaac ctcgaggaac gtgacgttct
     2521 cttcatcgac gagatccatc gtctcaaccc ggcggtcgaa gaaatcctct atccggctat
     2581 ggaggatttt cagctcgacc taatcatcgg tgaagggcct gccgcgcgtt cagtccgcat
     2641 cgacctgccc aagttcaccc tcgttggagc aaccacgcgc gccggcctgc tcacgacgcc
     2701 gctgcgggac cgcttcggca tacccattcg cctcaacttc tacacagtcg aggagctgga
     2761 gcaggtggtc ttgcgcggtg cgcgggtgct cggcgcaaag atcaatgccg agggagccag
     2821 agagatagcg cgacgctcgc gcgggacacc gcgcatcgcc gggcggctcc tccgccgcgt
     2881 gtgggatttc gccgtcgtct ccaaggcgaa ggtcatcgac gccgctgtcg cagataaggc
     2941 cttgcgcgca ctggaggtcg acagtgccgg cctcgatgcg ctcgaccatc gctacctcac
     3001 atgcattgcg accaactatg agggtggccc ggtcggcatc gagacgatcg cagcggcgtt
     3061 atcggagccg cgggatgcgc tcgaggaaat cgtcgagccg tacctgcttc agcagggctt
     3121 catcggccgc acgcctcgag gccggatcgt aactctca
//
LOCUS       DASUMG010000545         2194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1118572, whole genome shotgun sequence.
ACCESSION   DASUMG010000545 DASUMG010000000
VERSION     DASUMG010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118572"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..836)
                     /gene="istB"
                     /locus_tag="VFX46_09095"
     CDS             complement(75..836)
                     /gene="istB"
                     /locus_tag="VFX46_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX5600781.1"
                     /translation="MLTHPTHDRLIALGLTGMAKALEDQRRQPDVATLPFEERLAMMI
                     DREAIERENKRLERRLKAASLRQSAIVEDIDHKAPRGLDRALFQKIIAGEWIARHQNL
                     LVIGPTGVGKSWIACALGHKACRDDRSVLYHRVPRLFDALALARGDGRYARLLKNLAR
                     ADLLILDDWGLAPLTPEQAHDLLEIIDDRHGRGSTIVTSQLPVDHWHEVIGNPTIADA
                     ILDRLVHNAHRLTLKGDSMRKIAAKRSGLDASPAL"
     gene            complement(852..>2194)
                     /gene="istA"
                     /locus_tag="VFX46_09100"
     CDS             complement(852..>2194)
                     /gene="istA"
                     /locus_tag="VFX46_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX5600782.1"
                     /translation="LFTAPSFEEKPKRPPLDWPRLHEELKRRGVTLMLLWLEYRANEP
                     DGFGYSRFCGLYGDWRRSVSATMRQTHAAGEKLFVDFAGDTVAVFDPLTGDTRQAHIF
                     VSALGASNFTYAEARWSEGLPDWIGVHVGALAAIGGVPKAVVCDNLKAGVTNASRYEP
                     GVNRTYQDLATHYGFAILPTRVRKPRDKAKVEVAVQIVQRFVLARLRNRRFFSLDDLN
                     ITIRECVADLNAKIMRKLGKSRRELLETIERPALKALPTEPYRYAEWKRARVAPDYHV
                     EIAGHFYSVPSRLIREVVEARITDTTIEIFYRGTRVASHAFSPVRNRHTTIPEHMPSA
                     HRRYAEWTPARLMQEAEKIGPATLALFEAIMRAKPHPEQGFRACLGILRLAKGYGVER
                     LDAACRRGNDIGARSYGSIASILKHGLDRACREGQPPEGPPIRHGNIRGRGYYH"
BASE COUNT          369 a          662 c          761 g          402 t
ORIGIN      
        1 agggattcca attgaaggcg gccaccattc caatcgaagg cgggcagcat aggcccgtcg
       61 gcacgttcat ggggtcacag cgctggtgag gcgtcaagac cggatcgttt ggctgcgatc
      121 tttcgcatgc tgtctccttt caacgtgagc cggtgagcgt tgtgcacaag gcggtcgagg
      181 atggcatcgg cgatggtggg attgccgatc acctcgtgcc aatgatcaac cggtagctgg
      241 cttgtcacga tggtcgagcc gcggccgtgg cggtcgtcga tgatctccag aaggtcgtgc
      301 gcttgctctg gggtgagcgg ggcaaggccc cagtcgtcga ggatcaacaa atcggcgcgt
      361 gccaggttct tgaggagcct cgcatagcgg ccgtcaccgc gagcgagcgc cagcgcatcg
      421 aacagcctgg gcacgcgatg ataaaggacg gagcggtcgt cgcggcaggc cttgtggccg
      481 agcgcgcaag caatccaact tttgccgacg ccggttgggc cgatgacgag caaattctgg
      541 tgccgtgcga tccactcgcc ggcaatgatt ttttggaaca gcgcccggtc gaggccgcgc
      601 ggggctttgt ggtcgatgtc ctcgacgata gcggactggc gcaggctggc ggccttgagg
      661 cggcgttcga ggcgcttgtt ctcacgctcg atcgcctcgc gatcgatcat catggcgaga
      721 cgttcctcga aggggagcgt ggcgacatcg ggctggcggc gctggtcttc gagtgccttg
      781 gccatgccgg tgagcccgag tgcgatcagg cgatcatggg tgggatgggt cagcatcgtt
      841 tcctcctgtc ctcagtggta gtagccacgg ccgcgaatgt tgccgtgccg gatcggcggg
      901 ccttccgggg gctgcccctc gcggcaggcc cggtcgaggc catgcttaag gatcgaggcg
      961 atggagccgt agctgcgagc gccgatgtcg ttgccgcgcc ggcaggcggc atcgagccgc
     1021 tcgacgccat atcccttggc gagccgcaga atgccgaggc aggcgcggaa cccctgctcg
     1081 ggatgcggct tcgcccgcat gattgcctcg aataaagcga gcgttgccgg cccgatcttc
     1141 tcggcctcct gcatcaggcg tgcgggcgtc cactcggcgt aacgccggtg cgcactcggc
     1201 atgtgctctg ggatcgtggt gtgccggttc cgcacgggag agaacgcatg gctggcaacg
     1261 cgcgtgccgc gatagaagat ctcgatggtc gtgtcggtga tgcgcgcctc gactacttca
     1321 cggataaggc gcgaggggac ggagtagaag tggcctgcaa tctcgacgtg gtaatcgggg
     1381 gcgacacggg cgcgcttcca ctcggcatag cgataaggtt cggttggcaa cgctttgagc
     1441 gccggccgct cgatcgtctc caggagttcg cgccggctct tgccgagctt gcgcatgatc
     1501 ttcgcgttga gatcggcgac gcactcgcgg atcgtgatat tgagatcgtc gagtgagaag
     1561 aagcgccggt tgcgcaatcg tgccagcacg aagcgctgga caatttgcac ggccacttcc
     1621 accttggcct tgtcgcgcgg cttgcggacc cgcgtcggca ggatcgcgaa gccataatgg
     1681 gtggcaaggt cctgataggt gcggttgacg ccgggctcgt aacgcgaggc gttggtgacg
     1741 ccggccttga ggttgtcgca gaccaccgcc ttcggcacgc cgccaatggc agcaagggcg
     1801 ccgacgtgca cgccgatcca atcggggagc ccctcgctcc agcgcgcctc cgcataggtg
     1861 aaattggatg ctccgagcgc ggacacgaag atgtgggctt gccgcgtgtc accggtcagt
     1921 ggatcgaaca ccgccaccgt gtcgccggca aaatccacga acagcttttc gcccgccgca
     1981 tgcgtctggc gcatcgtcgc cgacacgctc cggcgccagt cgccgtagag gccgcaaaac
     2041 cggctgtagc caaacccatc aggctcgttg gcgcggtact ccagccagag cagcatcagc
     2101 gtcacgccac ggcgcttgag ctcctcgtgc aggcgcggcc aatcaagtgg cggacgcttc
     2161 ggtttctcct cgaacgaggg ggccgtgaac agcc
//
LOCUS       DASUMG010000546         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1118629, whole genome shotgun sequence.
ACCESSION   DASUMG010000546 DASUMG010000000
VERSION     DASUMG010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118629"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1893
                     /locus_tag="VFX46_09105"
     CDS             <1..1893
                     /locus_tag="VFX46_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600783.1"
                     /translation="TSITAIPHTGASQPADIYPHIEWRVENPFRFFIDPTDTEAHRTI
                     FLSLSDEEHDSPVLSAERKLGSRYQDGWASIVLGRTCWRADKSRHICPDGGDYINPKS
                     HRIVAELKGIPDASFVDCTWLTAPKGIGAPRGQAVTQPCNEPVLLDVPYPNGARITVE
                     IGGREVAATDATVTDLFIVGIGDSFASGEGNPDLPIRFSRERSVSYGAGLMTELTGYP
                     ARVGSWREIGDERFIQENARWLDQACHRSLYSHQLRAALQLSLEDPHRAVTFVGVACS
                     GAEITDGLFLRYMGNEWVPNPPRLSQISAVAEAQCGNHRPRKVSFPEAYHFNGRVPEL
                     KGVSLVKCDAEFARKIDLLMVSIGGNDVGFSRLVANAVLTDKSMLKWLGGWLGQIEGA
                     STAKEQLARLYWRYRALHRALQNILHIHESDRILLTAYPGLALLEDGTTVCPSGRAGM
                     DILKDFKLDEKKALEGSVLAEHLNELMRRAAREHGWTFVESHRKEFLGRGICAGWSDA
                     AFSTADDLRLPRKINGVWKPYNPADYRPYAPRQRWFRTPNDAFMTGNFHVPATLLQKV
                     LQMHTSWFQLVLAATYSGAFHPTAEGQAAIADAVVKKARQVLAKYERSNEMQHGFFTD
                     IPRFNP"
     gene            complement(1934..>3167)
                     /gene="trmFO"
                     /locus_tag="VFX46_09110"
     CDS             complement(1934..>3167)
                     /gene="trmFO"
                     /locus_tag="VFX46_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX5600784.1"
                     /translation="DWETNAVGLLHEEMRRADSLIMAAGDKHKLPAGGALAVDREAFS
                     AEVTARLSALPNVTIERGEVAGLPPEDWDSVIIATGPLTSPALSQAILELTGEDALAF
                     FDAIAPIVHYDTIDMSVAWRQSRYDKAGPGGTGADYINCPLNKEQYERFVDALLAGEK
                     TEFREWEKNTPYFDGCLPIEVMAERGRDTLRFGPMKPVGLSNPHKGGERPYAVVQLRQ
                     DNALGTLWNMVGFQTKLKYGEQVRIFRMIPGLENAEFARLGGLHRNTFINSPKLLDGL
                     LRLKVQPRLRFAGQVTGVEGYVESAAIGLLAGRFAAAERLGRKPVPPPPTTAFGALLN
                     HITGGHLSEDRIESGPRSFQPMNINFGLFPPLEPGEEGSGLKGKERGRAKKRAIALRA
                     LRDLDRWLADTSLRAAAE"
BASE COUNT          559 a         1017 c         1006 g          585 t
ORIGIN      
        1 acatcgatta cggccattcc ccataccggt gcaagtcagc ctgcagacat ctatcctcac
       61 atcgaatggc gggttgagaa ccccttccgt ttcttcattg atccaacaga taccgaggcc
      121 catcgcacga tattcctgtc gctctcggac gaggagcacg actcgccggt gctttccgcc
      181 gagcgcaagc tgggctcccg ctaccaggac ggctgggcct cgatcgtcct tggccgaact
      241 tgctggcgtg ctgacaagag ccgtcacatt tgccctgacg gcggagatta catcaatccg
      301 aagtcgcaca ggatcgtcgc tgagctgaag ggtattcccg acgcaagctt cgtcgactgc
      361 acgtggctga cggctccaaa ggggataggt gccccgcgcg ggcaggcggt gacccagccc
      421 tgcaatgagc cagttttgct cgacgtgccg tatcccaatg gcgcgcgcat caccgtcgaa
      481 atcggcgggc gcgaggttgc agccacggac gcaaccgtta cggacctgtt catcgttgga
      541 atcggcgaca gcttcgcttc aggtgagggt aacccggatt tgcccatccg tttctcgcgc
      601 gagcggtcgg tgagctacgg agccggcctg atgacggagc tcacgggcta tcctgctcgg
      661 gttggcagct ggcgggagat cggtgacgag cgcttcatcc aggagaatgc ccgctggctc
      721 gaccaggcct gccaccgctc cctctactcc caccagcttc gcgccgcgct ccagctttcg
      781 ctggaggatc cgcatcgggc ggtgacattc gtgggcgtcg cctgctcggg ggcagagatt
      841 actgacggtt tgttcctgcg gtacatgggc aacgagtggg tgccaaaccc gccgcggctc
      901 tcccagattt cagctgtcgc cgaggctcag tgcggcaacc atcggccccg caaggtgagc
      961 ttccctgagg cgtatcactt caacggccgg gttcccgagc tgaaaggggt gtcgcttgtc
     1021 aagtgcgatg ccgagttcgc gcggaagatc gaccttctga tggtctcgat cggcggcaac
     1081 gacgttggct tctcgcggct cgttgcaaac gcggtgctga cagacaagtc gatgttgaaa
     1141 tggcttggcg gctggctggg tcagatcgag ggtgcaagca ccgcaaagga gcaactcgcg
     1201 cgtctttatt ggcgctaccg ggcgctccac cgcgcgcttc agaacatcct ccacatccac
     1261 gagagcgatc ggattctgct gaccgcctat cctgggctag cgctgctcga ggacggaacc
     1321 acagtgtgtc ccagcgggcg agcgggcatg gatatactca aggacttcaa gctcgacgag
     1381 aagaaggcgc ttgagggcag tgtgctggcg gagcatctca acgagctgat gcgccgtgct
     1441 gctcgggagc acggttggac gttcgtcgaa agccaccgca aggagttcct cggtcgcggg
     1501 atctgtgccg ggtggagcga cgcagctttc tcgaccgccg acgacctcag gctaccgcgc
     1561 aagatcaacg gtgtttggaa gccatataac cccgcggatt atcggcccta tgccccccgt
     1621 cagcgctggt tcaggacgcc gaacgacgcg ttcatgaccg gcaacttcca cgtgccggcc
     1681 acgctgctgc agaaggtgct gcagatgcat acctcgtggt tccagctggt gctggccgcg
     1741 acatactccg gcgctttcca tccaacggcg gaaggtcagg cggccatcgc cgatgcggtc
     1801 gtcaagaagg cgcgccaagt gctcgccaag tatgagcgca gcaatgagat gcagcacggc
     1861 ttcttcaccg acattccgcg gttcaatccg taagctgctc tctttggcct gagcattcta
     1921 ggataaggcg ctcctactca gccgctgccc gcaggctcgt atccgccagc cagcggtcga
     1981 ggtctcggag cgcccgtaga gcgatggccc gcttcttcgc gcggccgcgc tccttgcctt
     2041 tgaggccgga gccttcctca ccgggctcca gcggcgggaa gaggccgaag ttgatgttca
     2101 tcggctggaa cgagcgcggc ccggattcga tgcgatcctc cgacaagtga ccgccggtga
     2161 tgtggttgag caaggcgccg aaggcggttg tcggaggagg cggcacaggc ttgcggccca
     2221 gccgctcggc cgcagcgaaa cgccccgcca agagtccgat agccgcgctt tcgacgtagc
     2281 cttcgacgcc cgtcacctgg ccggcaaagc gcaggcgtgg ctggaccttg agccgcaata
     2341 gcccgtcgag cagcttgggg ctgttgatga acgtgttgcg atgcaggccg ccgagccggg
     2401 cgaactcggc attctcgagc cccggaatca tccggaaaat gcgcacctgc tcgccgtact
     2461 tcagcttcgt ctggaagcca accatgttcc agagcgtgcc aagcgcattg tcctgccgca
     2521 gctgcaccac cgcgtagggc cgctcgccgc ccttatgcgg attggaaagg ccgaccggct
     2581 tcatcggccc gaagcgcaat gtatcccggc cgcgctcggc catcacctcg atgggcaagc
     2641 agccgtcgaa gtagggcgtg ttcttctccc actcgcggaa ctcggtcttc tcgccagcaa
     2701 gcagcgcgtc gacgaagcgc tcgtactgct ccttgttgag cgggcagttg atgtagtccg
     2761 cccccgtgcc gccggggccc gccttgtcat agcgcgactg ccgccaggcg acggacatgt
     2821 cgatcgtgtc gtaatgcacg atcggcgcga tggcatcgaa gaaggcgagg gcgtcctcgc
     2881 ccgtcagctc gagaatggcc tggctcaagg cgggcgacgt caacggtccc gtcgcaatga
     2941 tgacgctgtc ccaatcctcg ggtggcaggc cggcgacttc gccccgctcg atcgtcacgt
     3001 tgggtaaggc cgaaagccgc gcggtcacct cggcggagaa agcctcgcgg tccacggcga
     3061 gcgcgccgcc ggccggcagt ttgtgcttat cgccagcggc cataatgagg gagtcggcgc
     3121 gccgcatctc ctcatgcagc agcccgaccg cgttcgtctc ccagtcg
//
LOCUS       DASUMG010000547         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_557411, whole genome shotgun sequence.
ACCESSION   DASUMG010000547 DASUMG010000000
VERSION     DASUMG010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557411"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="VFX46_09115"
     CDS             complement(<1..1177)
                     /locus_tag="VFX46_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX5600785.1"
                     /translation="MSSHGGAETFDYVIIGAGSAGCVLANRLTADPTVTVALLEAGGP
                     DRNPWLHIPAGYFRTMFNPKVTWQFECGPEPHLDGRILPWPRGRVLGGTSAVNGLLYV
                     RGQAQDFDMWRQLGNAGWSYQDVLPYFKRAEDQERGADEYHGAGGPLGVSDVHMDNPL
                     CEAYIAACVAAGIPRNNDFNGPTQEGVGYYQLTTRNGRRCSTAVGYLKPVRSRPNLKI
                     ITEAQVKRIALDGRRATGVVYKLGGEMRTINARREVLLAAGAIGSPQLLQVSGIGPGS
                     VLKRAGVEVQHELPGVGENLQDHFQVRFVYDCSLPASLNDVWHSRLKQLRVGLEYIFN
                     RSGILTIGAGVVGVFAKTRPELEVPDVQIHFMPLSAERPGYGLHPFSGVTASVCQLRP
                     "
     gene            complement(1243..2127)
                     /locus_tag="VFX46_09120"
     CDS             complement(1243..2127)
                     /locus_tag="VFX46_09120"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX5600786.1"
                     /translation="MADTIAFVGLGAMGLPMARNLVQKQFRVVGYDVRPQAVEALVEA
                     GGHGAKSAREAAQGANFLVLMVVNADQAEEVLFANDALGALSPDATVILMATCPPDRV
                     REMAKRVEATGRSFVDAPVSGGVAGAASGTLTIMVGAASPVFAKSKPVLEAIGSNVYH
                     LGEEPGQGAVMKTINQLLCGVHIATAAEGLALAERAGVDPKLALQILSGSAASSWMLQ
                     NRGPRMVADDGQVTSALDIFVKDLGIVLEAGKSLRMGLPLAAAAHQLFVAASGMGLGG
                     KDDSQVIEIYRALRPKTS"
     gene            2349..>2636
                     /locus_tag="VFX46_09125"
     CDS             2349..>2636
                     /locus_tag="VFX46_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX5600787.1"
                     /translation="MIEQLNNEAEIFRTPCGEGDLVWRKWGTGAPVVLCHGGSGSWMH
                     WIKTIPALRQRYEVWAPDLPGLGDSAMPPPPFTPASSAEVLAAGIRTLLNEE"
BASE COUNT          493 a          905 c          757 g          481 t
ORIGIN      
        1 ccggcctgag ctggcacaca gaagccgtaa caccagagaa cgggtgcagc ccatagcctg
       61 gccgctcggc tgacaacggc atgaagtgaa tttgaacgtc tggcacctcg agctcaggcc
      121 gcgttttcgc gaacacccca acaacgccgg cgccaatcgt gagaattccg ctacgattga
      181 agatgtattc caagccgacc cttagctgct tcaggcggct gtgccaaacg tcgttcaggc
      241 tcgccggcaa tgagcagtca tagacaaaac gcacctggaa gtgatcttgc aggttctcgc
      301 cgacgcccgg cagttcatgc tggacctcga cgcctgcgcg cttcagaacg gaaccggggc
      361 cgatgcccga aacctgcagc aattgcgggc ttccgatcgc gccggctgcg agcagcactt
      421 cgcgccgggc attgatggtg cgcatctcac cgccgagctt gtaaacgacg ccggtggcgc
      481 ggcgcccgtc gagggcgatc cgcttcacct gcgcttcggt gatgatcttc aggttcggcc
      541 gtgagcggac cggtttgagg taaccgaccg ccgtcgaaca acgacggcca ttgcgcgtcg
      601 tcagctggta atagccaacg ccttcttggg tcggaccgtt gaaatcgttg ttccgcggaa
      661 tacccgccgc aacgcacgcc gcaatgtagg cctcgcacaa cgggttatcc atgtgtacgt
      721 cggagacgcc gagtgggccg ccagccccgt gatactcgtc cgctccgcgt tcctgatcct
      781 ccgcccgttt gaagtacggc aggacatcct ggtacgacca accagcattt ccgagctggc
      841 gccacatgtc gaagtcctgc gcctggccgc gcacatagag cagtccgttc accgcgcttg
      901 tgccccccag cacccgacca cgcggccagg gcaagatgcg cccatccaga tgcggctcgg
      961 gcccgcattc gaactgccac gtcaccttcg gattgaacat cgtgcggaaa tagcccgcgg
     1021 gaatgtgcag ccagggattc cgatccggcc cgcccgcttc gagcagcgca actgtcacgg
     1081 tcggatctgc agtcaaccgg tttgctagaa cgcaccccgc agatcccgcg ccgatgatga
     1141 cgtaatcgaa agtttccgcg cccccatggg agctcatgca tttcctccgc aattccgtcc
     1201 cggctctacc ggcactgccg atcactttct gtgcgcgctt gtttacgacg tcttcggcct
     1261 gagagcacga tagatctcga tgacctgact atcgtccttc ccgccgagcc ccattccgga
     1321 tgccgcaacg aacagctgat gggcagctgc tgcgagcggc aatcccattc tcagcgattt
     1381 cccggcttca agcacaatac ccagatcctt gacgaaaatg tcgagcgcac tcgtgacctg
     1441 cccgtcgtcg gccaccatcc gcgggccgcg gttctgcagc atccagcttg aagcggcaga
     1501 gccggagagg atttgaagcg cgagcttcgg atcgacacct gcgcgttccg ccagcgcgag
     1561 gccttccgcc gccgttgcga tgtgcacacc gcaaaggagc tggttgatgg ttttcatcac
     1621 cgccccttgt ccgggctctt cgccaaggtg gtagacgttg ctgccaattg cttcgagcac
     1681 cggcttcgat ttcgcgaaga ccggactggc cgctccgacc attatggtga gcgtgcccga
     1741 cgctgcgccg gcgacgccgc ccgacaccgg agcatcgacg aaactccggc ccgttgcctc
     1801 cacccgtttt gccatctccc gcacgcggtc tggcgggcag gtggccatca ggatcaccgt
     1861 cgcatccggc gacaatgctc cgagcgcgtc gttggcgaac agcacctcct cggcctgatc
     1921 cgcattgact accatcagga caaggaagtt cgccccctgc gctgcttcac gcgcgctttt
     1981 agccccgtgc ccaccggcct ccaccaaagc ttcgacggcc tgcggtctga catcgtaacc
     2041 gacgacccga aactgctttt gcacgagatt gcgggccatc gggagcccca tggctccaag
     2101 cccgacgaag gcgatcgtgt ccgccattct gccccacctt gtcgttgcct cctgcctccc
     2161 agctcaggtt gcgagccgag acttttgact aagcatgcaa cggcaggaac atattccgcc
     2221 agggccttga tcgcgcacgt ctccgccact cagcgcaaag ctgctcagcg ccgctgcgca
     2281 ttgagaaccg cctcacggtg ggctatgcga cggccccagc aacgtccgcc ccgacttcca
     2341 aggcagagat gatcgagcag ctcaacaacg aagccgaaat cttccgcacg ccctgcggag
     2401 aaggagatct ggtctggcgg aaatggggga ctggcgcacc ggtcgtgctt tgccacggtg
     2461 gctcgggctc ctggatgcac tggatcaaga cgatacccgc gctgcggcag cgttacgaag
     2521 tgtgggcacc ggatctccct ggcctcggcg acagcgccat gccgccaccg ccctttacgc
     2581 ccgcatcatc agccgaggtg ctggccgcgg gcattcggac gctcctcaac gaagag
//
LOCUS       DASUMG010000548         5609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_75245, whole genome shotgun sequence.
ACCESSION   DASUMG010000548 DASUMG010000000
VERSION     DASUMG010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75245"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFX46_09130"
     CDS             complement(<1..201)
                     /locus_tag="VFX46_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEX5600788.1"
                     /translation="MPSTSLPPREELLICFAHAAYRMKDAFLRRDTGINAIEVRSVED
                     LFAVAPQAHVIVASGFWRIELLD"
     gene            complement(294..1109)
                     /locus_tag="VFX46_09135"
     CDS             complement(294..1109)
                     /locus_tag="VFX46_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX5600789.1"
                     /translation="MRRWQKLLACLLMMPMLIGAPKAQTPEPEGSTAETGTRRTLLRF
                     VTDGDFPPFNYYDEEGILTGFNVDLARALCLEASTSCDIQARPWEALVPALNRGDADA
                     IIAGHKITPQLLQSVDFTDRYFHTPARFAARRDAEDFEVNPEGLDGRSIAVAKGTAHE
                     AYLKAFFRFSAIREFDTPELAREALMNGEVDLVFDDGIGLSFWVIGTSSRECCELRGG
                     PFYDPKYFGDGLAIAVSKRDQDLKHLLNQALKRVRESGRYEELVLRYFPNPVY"
     gene            complement(1274..1468)
                     /locus_tag="VFX46_09140"
     CDS             complement(1274..1468)
                     /locus_tag="VFX46_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600790.1"
                     /translation="MPGQSLDLIGFGDPVPGNEDFVRGNSPLPAEGDGLEQGLPVEQI
                     ADQEGVFSFGQIRGVLSEAL"
     gene            1505..3064
                     /locus_tag="VFX46_09145"
     CDS             1505..3064
                     /locus_tag="VFX46_09145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX5600791.1"
                     /translation="MKPGVVLLDGTATTPWEVARRIAELEATGKSVVLALPSEIADRS
                     PPNAPRLRLQRAITGLLHWRPVWSAGMPVWFWQRLALLAGGGFLVGQVVTAPDVAYTT
                     LFIVLTLAFTPIVVMRLVILALGLLPELGKSPRERIPDAELPVYSILVPMFREGEVLP
                     DLVAALSAIDYPAAKLDVLLVLESVDHETQKVAASLDLPGFMRVVVVPDSQPRTKPKA
                     LNYALQLARGDYVVVYDAEDAPDPDQLRKAISRFREAPAKVACLQGRLNIHNVRDSWL
                     TRQFTLEYTVLFDVILPGLARLGLPIPLGGTSNHFPRQVLDRWFGWDPFNVTEDADLG
                     IRLARTGARVEMLNSTTWEEAPARFGIWFRQRTRWLKGWMQTYLVHTRQPLRLMWDLG
                     WAAFLGFHLYSGGLVLSALVFPIFCIIVAVEVWQGVWLDLPMSIPSGALWTLAVFNLA
                     AAYLATLVGTVVAIWRRGRIWLLPAVLLMPFYWLLISLAAYRAAIQLATAPYYWEKTE
                     HRSRTRPRQAT"
     gene            complement(3142..3216)
                     /locus_tag="VFX46_09150"
     tRNA            complement(3142..3216)
                     /locus_tag="VFX46_09150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3182..3184),aa:Gly,seq:ccc)
     gene            3391..3606
                     /locus_tag="VFX46_09155"
     CDS             3391..3606
                     /locus_tag="VFX46_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600792.1"
                     /translation="MPVTFEDIVALKQRSEENKKAPADQRGYLALELIADTLILTLEQ
                     ELDEELADEEDEEEDEFEEEDEEEDEG"
     gene            3770..4747
                     /locus_tag="VFX46_09160"
     CDS             3770..4747
                     /locus_tag="VFX46_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX5600793.1"
                     /translation="MELSPPAGRRLAKFENPDWTASLEPRAVVPLVRLETLWINTGTL
                     CNITCRNCYIESSPRNDRLTYITAAEVSAYLDEIEDHHLPTREIGFTGGEPFMNPDII
                     PMLGDALRRGFQVLVLTNAMRPMQRPRIKEGLLGLLAEHDRRLKIRVSLDHYTQALHE
                     RERGAGTWIKTLQGLDWLSENGFNIAIAGRMCWGEDERALRAGYGKLIAERGWRIDPE
                     DPSQLTLLPEMDGSYDVPEITTRCWDILKKSPADVMCARSRMVVKRKGAAAPVVVPCT
                     LLPYEPAFEMGTTLAEAARADGGMFAKGAVKLCHVHCAKFCVLGGGSCS"
     gene            complement(4766..5152)
                     /gene="mscL"
                     /locus_tag="VFX46_09165"
     CDS             complement(4766..5152)
                     /gene="mscL"
                     /locus_tag="VFX46_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX5600794.1"
                     /translation="MSFIQEFKEFAMRGNVLDLAVAVIMGAAFGPVVTTLVEGIIMPP
                     IGYILAGIDFSALAITLPTPTGDGVEIKYGMFLNAVITFLITAFAIFLLVKAVNRFRR
                     PAPQAPAAESPTEVYLREIRDALVKK"
     gene            5422..>5609
                     /locus_tag="VFX46_09170"
     CDS             5422..>5609
                     /locus_tag="VFX46_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600795.1"
                     /translation="MFDGWAILALGIAYIGVLFAIAWTGDRTIRSRKGGEGRPLIYAL
                     SLGVYCTSWTFFGSVGLAA"
BASE COUNT         1115 a         1685 c         1680 g         1129 t
ORIGIN      
        1 atccagaagc tcgatccgcc agaaccccga tgcgacgatg acatgagcct gcggtgccac
       61 cgcaaacaga tcctcgacgg aacgcacctc gatggcgttg atgcccgtgt cgcgccgcag
      121 gaacgcatcc ttcatcctgt aggcggcgtg agcgaagcag atgaggagct cttcgcgggg
      181 cgggagcgag gtcgagggca tgaggctttg atcttccgag aattggaaca acaacggtct
      241 atgcccgcca gaatgcctca gggggatgct tcaggcaatt cggccgcagg cggtcagtag
      301 acaggattag ggaagtaccg gagcaccagc tcctcgtagc ggccgctctc acgaacccgt
      361 ttgagagcct gattgagcag atgtttcaga tcttgatctc gcttcgatac tgcgatggct
      421 agtccgtcgc cgaagtactt cgggtcatag aacggccctc ctctcagctc gcagcattcc
      481 cgagacgatg ttccaatcac ccagaagctg agcccgattc catcgtcgaa cacgaggtcg
      541 acctcgccgt tcatcagcgc ctcgcgggca agttccggag tgtcgaactc cctgatggcg
      601 ctaaatcgga agaaggcctt caggtaagcc tcgtgggctg ttcctttcgc caccgcgatc
      661 gatcgtccat ccaggccctc tggattgacc tcgaaatctt cagcgtcgcg cctcgccgcg
      721 aagcgagcgg gcgtatggaa gtaccgatct gtgaagtcaa cagattgaag cagttgtggc
      781 gtgatcttgt gcccggcgat gatcgcatcc gcatcgcctc ggttgagtgc agggacgagc
      841 gcttcccacg gcctggcctg gatgtcgcag gacgtgctcg cctcgaggca caatgcgcgg
      901 gctaggtcca cgttgaagcc cgtcaggatg ccttcctcgt cgtaatagtt gaatggcggg
      961 aaatcgccgt cggtgacgaa gcgcagcaac gtgcggcggg ttccggtttc cgcagtggac
     1021 ccctctggct ccggcgtctg ggctttgggg gcgccgatga gcattggcat catcaataga
     1081 caggccagca gcttctgcca tcgcctcatc gtaaagcgct cctgttgaaa ccggcttcca
     1141 gcataatcga cttaccctac taggttgcta cctaggtagc ggccgagcgt cggacactta
     1201 atgtaagcga gcctctcaag cccaacatgt tttccagctc gcgctcaccg tgaaccatca
     1261 cgatggggcc ctatcaaaga gcctcactca agacaccacg gatctgaccg aagctgaata
     1321 cgccttcctg gtcggcaatc tgctcgaccg ggagaccctg ctccaagcca tcgccctcag
     1381 ccggcagtgg ggagttgccc ctcacgaagt cctcattgcc cgggactgga tcaccgaacc
     1441 caattaggtc gaggctttgg ccaggcatct cggagtgcgc gcactgagcc aaatcgagat
     1501 ccgattgaag ccaggagttg tgcttctcga cggtaccgca acaacgccct gggaagtcgc
     1561 gcgtcgcatt gccgagctcg aggccaccgg caagtcggtc gtgctggcgt tgccaagcga
     1621 aattgccgac cggtccccgc ctaacgcacc gcgcctgcga ttgcagcggg ccatcacagg
     1681 ccttctccac tggcgcccgg tgtggtcggc cggaatgccg gtctggtttt ggcagcgatt
     1741 agccctcctg gccggagggg ggttcctggt gggtcaggtc gtaactgcgc ctgatgttgc
     1801 ctacaccacc ttgttcattg tcctcaccct tgcgtttacg ccgatcgtcg tgatgcgctt
     1861 ggtgatcctt gcgctggggt tgctccccga actagggaaa tccccgcgag aacgcatccc
     1921 cgatgcggag ctaccggttt actccatcct cgtcccgatg tttcgcgagg gggaagtcct
     1981 gccggatctc gtggctgcat tgtcggcaat cgactatccg gccgccaagc tcgacgtgtt
     2041 gctcgttctc gaaagcgttg atcatgaaac tcagaaggtc gcggcctcct tggaccttcc
     2101 cggtttcatg cgcgtcgtcg tcgtgccgga ctcgcaaccg cgcacgaagc ccaaggcact
     2161 caattatgcg ctgcaactgg cgcggggcga ttacgttgtc gtctacgatg ccgaggacgc
     2221 gcccgatccc gaccaactcc gcaaggcgat atcccgcttc cgtgaggctc cggcgaaagt
     2281 ggcttgtctg cagggcaggc tgaatattca taatgtgcgc gatagctggc taacacgtca
     2341 gtttacgctc gagtacaccg tccttttcga tgtgatcctg ccgggattgg cgcggctggg
     2401 cttgccgatc ccgctcggcg gcacctcgaa ccacttcccc cgtcaagtgc tcgaccgttg
     2461 gtttggctgg gaccccttca acgtcacgga ggatgccgac ctgggcataa ggctcgcgcg
     2521 caccggcgcg agggtcgaaa tgctgaactc cacgacctgg gaggaagcac cagcccgatt
     2581 cggcatctgg ttccggcagc gcacccgatg gctaaagggg tggatgcaga catatctcgt
     2641 gcacacacgc cagccgctgc gattgatgtg ggacctcggg tgggcagctt tcctaggctt
     2701 ccatctctac tccggcgggc tcgtcctgtc ggcgctcgtg ttccccatct tctgcatcat
     2761 cgtggccgtg gaggtctggc agggagtgtg gctggatctt ccgatgtcaa ttcctagtgg
     2821 cgcgctctgg accctggctg tcttcaacct agccgccgcc tacttggcta cacttgtcgg
     2881 taccgtggtg gcgatctgga ggcgcggccg aatctggctc ctgccagctg tgctcctcat
     2941 gccgttctat tggctgttga tctcgcttgc tgcttaccgc gctgccattc agctcgcgac
     3001 ggcgccttat tactgggaaa agactgagca tcgttcccgc acccgccctc ggcaggcgac
     3061 gtaggtaagg tcgccccaat agcggtcgtg gcgccagcaa acccactcag cgagaggtaa
     3121 ccggcggaag attgaagaaa ttggagcggg cgaagggaat cgaaccctcg acactcagct
     3181 tgggaagctg atgttctacc actgaactac gcccgcaaga tcagcgagat tgcttcgtgc
     3241 gtcactataa tgggcggctc ctgcgcgccg cgcaagaggc acctcagggg ctgtcgttgc
     3301 atgcctcaca ggcgagagca ccggctcgcg ctaacccccg gaatcagtct agccttcagc
     3361 cgaagctgta ccgcctgaga ggagctgatc atgccagtta cgttcgagga tatcgtggca
     3421 ctcaagcagc gctcggaaga gaacaagaag gctccggccg accaaagggg ctatctggcc
     3481 ctggagctca tcgcggacac tttgatcctc acccttgagc aagagctcga cgaagagctg
     3541 gcggacgaag aggatgaaga ggaggacgag ttcgaggaag aagacgaaga ggaagacgag
     3601 ggctagacct acagcgcgag gcgatgcgct ggcgtagtcc ggcaaccacc actagccaca
     3661 cttcttcccg cctcgcgcag gattgatcga aggttgagcg ggtggcacaa agccttgtgg
     3721 tagtgtctcg cggctcctga ccacccctct ccggaggtct tccgtagcta tggaactctc
     3781 gcctccagct ggtcgtcgcc tcgcgaagtt tgagaaccct gactggaccg catccctcga
     3841 acccagggcc gttgtgcctc tagtgcggct ggagacgctc tggatcaata ccggaacgct
     3901 ctgcaatatc acctgccgca actgctacat cgagtcgagc ccaagaaacg accgtttgac
     3961 ctatattacc gccgccgagg tctcggcata cctcgatgag atcgaggacc atcatctccc
     4021 gactcgggaa atcggcttca ccgggggcga gccgttcatg aacccggaca tcataccgat
     4081 gttgggagac gcgctgagac gcggctttca ggtcttggtg ctgaccaacg ccatgcggcc
     4141 catgcaacga ccacggatca aggaaggcct tttggggctg ctggccgagc atgaccgtcg
     4201 gctgaagatt cgcgtcagtc ttgatcatta cacgcaggcg ttgcatgagc gcgagcgcgg
     4261 cgcagggaca tggatcaaaa ctctgcaggg gctcgactgg ctgtcggaaa atgggttcaa
     4321 catcgccatc gcgggccgga tgtgctgggg cgaagatgag cgcgcattga gggccgggta
     4381 cggcaaactg atcgcggaga ggggatggcg gatcgatccg gaggacccaa gccagctcac
     4441 gctgctgcca gagatggacg gcagctatga cgtaccggaa atcacaacca gatgctggga
     4501 tatcctgaag aaatcacccg ccgacgtcat gtgcgcgcgg agccgtatgg tcgtgaagcg
     4561 aaagggcgca gcggctccgg tcgtcgtgcc gtgtacgctg ctcccctatg agcccgcctt
     4621 cgagatggga acaacgctgg cagaagccgc acgggccgat ggcggaatgt tcgccaaagg
     4681 cgccgtgaag ctttgccatg tccactgtgc caagttttgc gtgcttggag gaggaagctg
     4741 ctcctaagct ccttccgatt gccacctact tcttcaccaa ggcgtcgcga atctcacgca
     4801 gatagacctc agtcggcgat tcggctgcgg gcgcttgagg tgccggccgg cgaaaccggt
     4861 tcaccgcctt gaccagcagg aaaatggcaa acgctgtaat taggaaggtg ataaccgcgt
     4921 tgagaaacat tccatatttg atctcaacgc catcccccgt cggcgtcgga agtgtgattg
     4981 ctagcgccga gaaatctatg cctgccaaga tatacccgat tggcggcatg atgatcccct
     5041 cgacgagtgt ggtcacgacg ggcccaaatg ccgcccccat gatcacggca acagcaaggt
     5101 ccagaacgtt gccgcgcatc gcaaactctt tgaactcctg aataaagctc attgttggcc
     5161 ctccttctcc tgccaagcag gcatcgcgcc tacgcagcta aacgaatcct cttgccggac
     5221 gggaggctag ttcaggctca gcaaactgta aatcttgacc atgagctgtg ttagtctggg
     5281 cctactttgc aggcatagag agcagggatt gcgcaaagtc ctgggttttg tgcaacatta
     5341 aaagctcatg cgcgcggcga tcaaagctgg gcctatgagc aggtaacttg atgtgtttac
     5401 cagacgcgaa ggccagcgtt tatgttcgac ggttgggcca tactagcgct tgggatcgcc
     5461 tacatcggcg tcctgttcgc aatcgcctgg accggtgacc ggaccattcg ttcacgcaag
     5521 ggcggtgagg gccggcctct catctacgca ctttcgcttg gcgtctactg cacatcgtgg
     5581 accttcttcg gaagcgtcgg gcttgcggc
//
LOCUS       DASUMG010000549         2402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_316207, whole genome shotgun sequence.
ACCESSION   DASUMG010000549 DASUMG010000000
VERSION     DASUMG010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316207"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..965
                     /locus_tag="VFX46_09175"
     CDS             96..965
                     /locus_tag="VFX46_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX5600796.1"
                     /translation="MPAAGASSTFRAGLVQMCTGRDVARNLNELSRLVREAAAAGASY
                     VQTPEVSVLMERDRARLFVETRPEEGNPAIAHCQALARELGIWLHLGSMGVLVKPDKI
                     ANRSYLFTPEGRIAARYDKIHMFDVELPGGESYRESRNFEPGSKAVLVDLPWGTLGLT
                     ICYDLRFPALYRALAKAGAQLIAVPSAFTVPTGQAHWHVLLRARAIESQCFILAAAQV
                     GEHESGRTTYGHSLILSPWGEILAEADGEGPSVIVADIDLKAVEEARRRVPSLQHDRA
                     FEVVHSSSDAHAS"
     gene            971..1417
                     /locus_tag="VFX46_09180"
     CDS             971..1417
                     /locus_tag="VFX46_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006328568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEX5600797.1"
                     /translation="MIRYSLQCHNNHTFEAWFSNSGDFDKQVKRNLVSCPQCNSTRIA
                     KALMAPNIAPSRSKSKEMAVASPEATAEAQKRRELMALLRRLRAEVEKNAEYVGPRFA
                     EEARKMHYDEVEKRGIYGEATLEEARELHEEGIEFYPLPRLPEDHN"
     gene            1604..>2402
                     /locus_tag="VFX46_09185"
     CDS             1604..>2402
                     /locus_tag="VFX46_09185"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX5600798.1"
                     /translation="MGYKVAVVGATGNVGREMLNVLAERRFPADEVFPIASRRSIGTE
                     VSYGDKTLKCFDIEQFDFKKADFCLMSAGSAVSKQWAPRIAAAGCTVIDNSSAWRYDQ
                     DVPLIVPEVNADAVQGFRKKNIIANPNCSTAQMVVVLKPLHDYATIKRVVVATYQSVS
                     GAGKEAMDELWHQTKGKYVPGQEVEPKNFPKEIAFNVIPHIDVFMEDGYTKEEWKMLA
                     ETKKILDPKIKVTATCVRVPVFVGHSEAVHIEFEKPITAEEARNVLRE"
BASE COUNT          510 a          665 c          763 g          464 t
ORIGIN      
        1 catcatgtcg gtggatgcga cgatctctat gagctcgacc ggaaaggcga gctcgataag
       61 ttgctgtcga gcgtacacga cgcgggggga tgagaatgcc cgctgcggga gccagcagca
      121 cattcagggc cggtctggtg cagatgtgta ctggccgcga tgtggctcgg aatctcaatg
      181 agctgagtcg gctggtgcgc gaagcggcgg ctgcgggagc cagctatgtt cagaccccgg
      241 aagtctccgt gctcatggag cgggaccggg cgcggctgtt cgtcgagacg cggcctgagg
      301 aaggcaaccc cgcgatagcc cactgccagg cgctggcccg cgaactgggt atctggctgc
      361 acctgggctc gatgggcgtc ctggttaagc ctgacaaaat cgcaaatcga tcctatctct
      421 tcacccccga gggccgaatt gcggcgcgct acgataagat ccatatgttt gatgtcgagt
      481 tgccgggtgg cgaaagctac cgcgaaagcc gcaatttcga gcccggcagc aaggctgttc
      541 tggtagatct gccatgggga acattggggc tgacgatctg ctacgacttg cgatttcccg
      601 ctttgtatcg agcgctcgcc aaagcaggtg ctcagctcat agcagtgccg tccgcattca
      661 cggttccaac tgggcaggcc cactggcatg ttctgctcag agcccgcgcg atcgagtctc
      721 aatgcttcat tctcgctgcg gcccaggtcg gcgaacatga aagcggccgg accacgtatg
      781 ggcacagtct cattctatcg ccgtgggggg aaatccttgc agaagccgat ggcgaggggc
      841 cgtccgtcat agtcgcggac atcgatctga aggcagtcga ggaagcgcgc cggcgcgtgc
      901 cgtcgcttca acatgatcgg gcgttcgagg tggtccacag ctcgtctgat gcacatgcga
      961 gttgaaggct atgatacgct acagcttaca atgccataac aaccatacgt tcgaagcttg
     1021 gttcagcaac agtggcgact tcgacaagca ggtgaagcgc aatctcgttt cctgcccgca
     1081 atgcaactct acgcggatcg ccaaggcctt gatggcgccg aacattgcgc cgagccggtc
     1141 caaatcgaag gagatggccg tcgccagtcc tgaggccacc gctgaggcac aaaagcgtcg
     1201 tgagctcatg gccttgcttc ggaggcttcg cgccgaggtc gagaaaaacg cggagtacgt
     1261 cggaccaagg ttcgcggagg aagctcgcaa gatgcactac gatgaagtcg agaagcgggg
     1321 gatctacgga gaggcgaccc tggaagaggc tcgggaactg cacgaggaag ggatcgagtt
     1381 ctatcctttg ccgagacttc cggaggacca caactgatcc tggcctccag ctggtggcca
     1441 acaaagctaa tttttacggt cgcatgacat cgtgagtccg cgtctgcgca cgcacggcgc
     1501 gcgtcttttt gggttgcatc tattgattcc tggctaggat cgtctaatcc gtgcgcgggg
     1561 tcggcacagt cgtcggcccg attttacgag agaaagatca gtcatgggct acaaggtcgc
     1621 tgttgttggc gctacgggca acgtaggccg tgagatgctg aatgtcctgg cggagcgtcg
     1681 ctttcctgcg gacgaggtct tcccgatcgc gtcgcgtcgc tccattggca cggaggtcag
     1741 ctacggcgac aaaacgctca aatgcttcga catcgagcaa ttcgacttca agaaggccga
     1801 cttctgcctg atgtcggcgg ggtccgccgt atccaagcaa tgggcgcccc gcatcgccgc
     1861 ggccggctgt acggtcatcg ataactcgtc ggcatggcgc tacgaccagg atgtgccatt
     1921 gatcgtgcct gaggtgaatg ccgacgcggt gcagggcttc aggaagaaga acatcatcgc
     1981 caatccgaac tgctcgaccg cgcagatggt cgtcgtgcta aagccgttac acgactacgc
     2041 gacgatcaag cgagtggtcg ttgcgaccta ccaatccgtt tccggcgccg gcaaggaggc
     2101 catggacgag ctgtggcacc agaccaaggg caaatatgtt cccggccaag aggtcgagcc
     2161 gaagaatttt ccgaaggaga tcgcgttcaa cgtcattcct cacatcgacg tcttcatgga
     2221 ggacggctac acgaaagagg aatggaagat gctggccgag acgaagaaga tccttgatcc
     2281 gaaaatcaag gttaccgcca catgcgtgcg cgtgccagtc ttcgttgggc actcggaagc
     2341 ggtgcatatc gagtttgaga agccgatcac ggccgaggag gcgcggaatg tactgcggga
     2401 gg
//
LOCUS       DASUMG010000550         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_558000, whole genome shotgun sequence.
ACCESSION   DASUMG010000550 DASUMG010000000
VERSION     DASUMG010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558000"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VFX46_09190"
     CDS             <1..612
                     /locus_tag="VFX46_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600799.1"
                     /translation="AKDSRHITSVGIMPSPVRSIVPLVVAALISGVLSYATEGLPRLS
                     HSIEMQLTAYLINPKLLLPGVWFGLVTGALAWRFGSRGLMGAALAFALTWVGWQLAVQ
                     AGIATFHQAGVLTPVETSRIALAGFAGGAVGAIVTFLGVRLAVPMPRTMVALVATVVT
                     GSVFGLLLPWSTTRQSAGLLLYAAWQPAVVAVMSYFAARKPAL"
     gene            708..971
                     /locus_tag="VFX46_09195"
     CDS             708..971
                     /locus_tag="VFX46_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003851381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX5600800.1"
                     /translation="MSILAWIVVGLIAGWIAEQITGREHGLLTNLLVGIIGAFVGGFL
                     FSTLLGFEYREGFNIASIVVATVGAIVFLFILSWFRGEQRTIR"
     gene            complement(984..1871)
                     /locus_tag="VFX46_09200"
     CDS             complement(984..1871)
                     /locus_tag="VFX46_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX5600801.1"
                     /translation="MKPTILTCAVTGNFPTREQNPNLPVTPEEIANECIAAAKAGAAI
                     CHIHVREPETGRPSMKLEYYREVVQRLRSSDVDLIINLTTGPGGRFIPSDDEPAKAAP
                     GTMLTTPEIRTQHIVELKPEMCSLDLNTMWFGGGAVINSPRNIRIMAERIYAAGVKPE
                     LEVFDTGDIMLSRDLFAEGTLRTPALFQIVTGVKYGMAATPESLCYAKSLLPMDCEWA
                     AFGAGRMAFPMLAQAFILGGHCRIGMEDTVHIAKGQLAPSNAALVEKAVRIIENLGGK
                     IATPAEARQILNLPERRAN"
     gene            complement(1962..2393)
                     /locus_tag="VFX46_09205"
     CDS             complement(1962..2393)
                     /locus_tag="VFX46_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014762590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX5600802.1"
                     /translation="MDEIAARRAFETALKTQQPAFEKLFLARFFGLEFTYTDDACRVE
                     FPVSDFMFNSQGTVHGGVTAFVLDSAMGHLLKHAVGPGTTLEMKVQYLAPVRPPRATC
                     EAKFLRKGQRIAFLEARLWDGEGTLAAVATSTWRVNRAEAQ"
BASE COUNT          465 a          823 c          803 g          558 t
ORIGIN      
        1 gcgaaagaca gccgtcacat cacatcggtg gggatcatgc cgtctcccgt tcgcagcatc
       61 gttccgctgg tcgttgccgc actgatttcc ggcgtcttga gctacgcgac cgagggcttg
      121 ccgcggttgt cccacagcat cgaaatgcag ctcacggcgt acctcatcaa tccgaagctg
      181 cttctgccgg gagtgtggtt cggcctggtg acaggagcgc tggcatggcg ctttggatct
      241 cgcgggctga tgggggcggc gttggcgttt gctctgacat gggtaggctg gcagctcgcg
      301 gttcaagccg gcatcgccac gttccatcag gcgggcgtcc tcacaccggt cgaaacaagt
      361 aggattgccc tcgccggatt tgccggtggt gccgtggggg cgatcgtcac gttcttgggt
      421 gtgcgtttag cggtgccaat gcctcggaca atggtagccc tggtagccac cgtcgtgacc
      481 ggttcggtat tcgggctgct cctgccatgg tccacgacgc ggcaaagcgc gggattgctg
      541 ctctacgctg cctggcaacc cgccgtcgtg gccgtcatgt cttacttcgc cgcacggaag
      601 cctgctctat agcgcgactt atattcatcc cgacgggcta gaagcggtga acttgttggg
      661 gcttgaggcg ttcctggtga acgctcgttt caacaggaga tgccgcagtg agtattctgg
      721 cctggattgt tgtcggccta atcgccggct ggatagccga gcaaattacc ggcagggagc
      781 atggactgct aacgaatctg ctcgtcggca tcatcggagc gttcgtaggc ggattcctgt
      841 tcagcacgct actcggcttc gagtacaggg aaggcttcaa catcgcctcg atcgtcgttg
      901 cgaccgttgg cgctatcgtc ttcctcttca tcctgagttg gttcaggggc gagcagcgca
      961 cgatccgctg acccgcccga aactcagttt gcgcggcgtt ccggcaggtt gaggatctgc
     1021 cgggcttccg cgggggtcgc gatcttgccg ccgaggttct ctatgatgcg gacggctttc
     1081 tcaacgagag ccgcgttgct cggcgcgagc tgccccttgg cgatgtgaac cgtgtcctcc
     1141 atgccgatgc ggcaatggcc accaaggatg aaagcctgcg ccagcatcgg gaacgccatg
     1201 cgacctgcgc cgaacgccgc ccactcgcaa tccattggca gcagcgactt ggcgtagcat
     1261 agcgattccg gcgtcgccgc cattccgtac ttcacgccgg tgacgatctg gaataacgcg
     1321 ggggtccgca gggtcccttc agcgaagaga tccctcgaaa gcatgatgtc gccggtgtcg
     1381 aacacctcca gctccggctt caccccggcg gcgtaaatgc gctccgccat gatccgaata
     1441 ttgcgcggcg agttgatcac ggcaccgccg ccgaaccaca tcgtgttgag atcaaggctg
     1501 cacatctcgg gtttcagctc aacgatgtgc tgggtgcgaa tttccggagt cgtgagcatc
     1561 gtgcccggcg cagccttggc cggctcgtca tcgctcggga tgaaacggcc gcccggaccc
     1621 gtcgtcagat tgatgatgag gtcgacgtca ctcgagcgca gccgctgaac cacctcgcga
     1681 tagtattcga gcttcatgct ggggcgccct gtttccggct cgcgcacatg gatgtggcat
     1741 atcgcggccc ccgcctttgc cgcagcgatg cattcgttgg cgatctcctc cggtgtgacc
     1801 ggcaggttgg ggttctgctc cctggtcgga aagttacccg tcaccgcgca ggtcaggatc
     1861 gtcggcttca tctctcctcc tcccgtcatc gccgcttcac ggcttgctgg cgctctcggc
     1921 gggcgccgtc tcaacgatag tgacccagcg cacgtcccct gtcactgcgc ctcagccctg
     1981 ttcacccgcc acgtcgaggt cgcgaccgcg gccagtgtac cttccccgtc ccagagccgc
     2041 gcctccagaa aagcgatacg ttgtcccttg cgcaggaatt tcgcctcaca cgtcgcgcgg
     2101 ggtgggcgca cgggtgcaag atactgcacc ttcatctcca aagtcgttcc cgggccgact
     2161 gcatgcttca gaaggtggcc catcgccgag tccagcacga aagctgtgac accaccgtgt
     2221 actgtgcctt gcgaattgaa catgaaatcg gaaaccggga actccacccg gcaggcatca
     2281 tcagtgtagg tgaactcgag accgaagaac cgcgccagga acaacttttc gaaggcaggt
     2341 tgctgcgttt tcaacgccgt ctcgaaggct cggcgagccg cgatctcgtc catgctacca
     2401 agctttatca ggagattgac gcgactatgc tcaattcaga ttggattgga ccgtcggccg
     2461 tctcgatgat tggtacgtga cgtctcaatt ctctgagcaa gctagcgacg gcagcacccg
     2521 actgtcaacc aatggatcat acaatcctgg ctacccaccg tgagaggctt ccgggcagta
     2581 tgaggaagcc gagaccatca tgagcattcg gcacggtgcg gcgagcaggc gtccattttc
     2641 ctgagcgca
//
LOCUS       DASUMG010000551         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_558025, whole genome shotgun sequence.
ACCESSION   DASUMG010000551 DASUMG010000000
VERSION     DASUMG010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558025"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VFX46_09210"
     CDS             complement(<1..1349)
                     /locus_tag="VFX46_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEX5600803.1"
                     /translation="MPSLTIAEQTRLARLATDRVRRAAVARLLSSRLLRWRYGAPVAD
                     ELLIVPQELRTADPSFADEIAIGQFGFAGAVAYIGSGSPFDLKPPSPEWERELHGFGW
                     LRHLRAAETDEAREQAVDLVLGWIRKHGSGGGVAWEPPVVARRLISWISSAPLLLDDV
                     DESTYDRIADSLGDQLIRLSATWRDAPAGYPRLLALTALVLADLCVAGHDRHLNEIME
                     AFSQELETQVLPDGGHISRNPGIPVELLLDLLPLRQCFAVRDRPAPPALGAAIERMLP
                     FVRFMRLGDGSLARFNGMGSPNLDLVATVLAYGEPLTRLPAHAPASGYARLARGGTVL
                     IADVGSPPKLELAGEAHAGCLSFEMSSGRCPVLVNCGAPGPADQDWRAASRATASHNT
                     LCLGGKSSSRLVLHNLLETLVGSAPIRFPDRVGVRIRDLPDGGIQLKAHHNGYHRQFG
                     "
     gene            complement(1517..>2472)
                     /locus_tag="VFX46_09215"
     CDS             complement(1517..>2472)
                     /locus_tag="VFX46_09215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX5600804.1"
                     /translation="LRRVAERGAEIAAGQDPVRLNIPEWLWKRWSVAYGDDTVRQIAE
                     ASLREAPLDVTVKSDAEGWSRRLGGVLLPTGSIRLVPEGRIENLPGFEDGAWWVQDAA
                     AALPAKLLGGVAGKQVADLCAAPGGKTAELAAAGAHVTAVEVDPKRIERLNANMARLQ
                     LEAEIVEADAAEWQPGTRFDDVLLDAPCLSTGTIRRHPDILHLKRESDLERLITLQAR
                     LLDNAARLVKPGGLLVYCTCSLEPEEGPQQIERFLSRTDEFERVAVQPGEAGIAAEWI
                     TADGDLRTFPFHMPLEPEALSGMDGFYAARLRRRSGGVAAE"
BASE COUNT          486 a          806 c          792 g          388 t
ORIGIN      
        1 ccgaactggc gatgatagcc gttgtggtgc gccttcagct gaatgccacc gtctggaaga
       61 tcccggattc gcacgccaac acgatcggga aagcggatgg gagccgagcc caccaacgtc
      121 tcgagaaggt tgtgcaggac caatctggac gaggatttgc cgccgagaca cagcgtgttg
      181 tgtgaagcgg tggcccgtga ggcggcgcgc cagtcctgat cggcgggacc gggtgctcca
      241 cagttgacca gcaccggaca ccggcccgag ctcatctcga atgaaaggca accggcgtgc
      301 gcctcccctg ccagctcgag cttcggcggt gagccgacat ctgcgatgag caccgtgcca
      361 ccgcgggcga gcctcgcata gcccgaggca ggcgcatgag ctggcaagcg cgtaagaggt
      421 tcgccgtacg ccagaacagt ggcaacgagg tctagattag gcgagcccat gccgttgaac
      481 cgggccagag acccgtcgcc taagcgcatg aagcggacga aaggaagcat gcgctcgatc
      541 gcggcaccga gcgctggtgg cgccggacga tcgcggacag caaagcactg ccgcaaaggc
      601 agcaggtcca gcaacagctc gacaggaatg cccggattgc ggctgatgtg tccgccgtcc
      661 ggcagaacct gcgtctcgag ctcctggctg aaggcctcca tgatttcgtt caagtgccga
      721 tcatgtccag ccacacaaag atcggcgaga acaagagccg tcagcgcgag caaacgcgga
      781 taaccagccg gcgcatcgcg ccaggttgcg gagagccgga tcagctggtc gcccaggctg
      841 tcggcgatcc gatcgtaagt cgactcgtcg acgtcatcca aaagcagggg agcgctcgaa
      901 atccaggaga tcagccgccg agcgacgacc ggcggctccc acgcgacacc gcctcccgag
      961 ccgtgcttgc gaatccagcc gaggacgaga tccacagcct gctcacgcgc ctcgtccgtt
     1021 tccgctgcgc gcaggtggcg caaccagcca aagccatgca gctcccgctc ccattcgggg
     1081 ctcggcggct tcaggtcgaa cggcgagccg ctgccgatgt aggcaaccgc gcctgcgaag
     1141 ccgaactgcc cgattgcgat ttcatcagcg aagctggggt cggctgttct cagctcctgc
     1201 ggcacgatca gcagctcgtc cgcgaccgga gcgccatagc gccaacgcaa cagccgcgaa
     1261 cttagcaggc gcgcaacggc agctcgacgc acccggtcgg tggccaaccg ggcgaggcgt
     1321 gtctgctcag cgattgtgag cgaaggcacg agcggctcct gcagctaggc tctgacgtgc
     1381 aagtgctcag gccaatgatg aacacgtcag ccgatcacgc caggagatat ccttaacgcg
     1441 gcctgctcac aagaaaggtg aagatgccgg tgtatggctt gtgggcgact tgtggaagga
     1501 atggcacagt gcgcagctac tcggcagcaa ccccgccaga ccgccgtcgc aagcgggccg
     1561 cataaaagcc atccatcccg gaaagggcct ctggctcgag tggcatgtgg aatgggaagg
     1621 tgcgcaagtc accgtcggcc gtgatccact ccgccgcaat gccggcctct ccaggctgga
     1681 cggcaaccct ctcaaactcg tccgtccttg agagaaaacg ctcgatctgt tgcggcccct
     1741 cctccggctc cagcgaacag gtgcagtaga cgaggagccc gccgggtttc acgagccgag
     1801 cagcgttgtc gagcagtcgc gcttgcaggg taatgagccg ttcgagatcg ctttcgcgct
     1861 tgaggtgcag gatatctggg tggcgccgga tcgtgccggt ggacaggcac ggcgcatcca
     1921 gcaatacgtc gtcgaagcgt gtgcctggct gccattccgc cgcatcggct tcgacaatct
     1981 ctgcctcgag ctggaggcgg gccatgttgg cgttcagccg ctcgatcctc ttcgggtcga
     2041 cttcaacagc cgtgacatgg gcaccggcgg ctgcaagttc ggccgttttg ccgcccggtg
     2101 ccgcgcagag atcggccacc tgtttgccgg cgacgccccc aagaagcttc gccggaagag
     2161 ctgcagcagc gtcctgcacc caccatgcgc catcctcgaa gccaggcaag ttctcgatgc
     2221 gcccctccgg cacgaggcgg atagagccgg tgggcagcag cacgccgccc aatctgcggc
     2281 tccagccttc agcgtccgac ttcacggtga cgtcgagcgg cgcttcccgc aaagatgctt
     2341 ccgcaatctg acggacggta tcatcgccgt aagcgactga ccagcgtttc cagagccatt
     2401 cggggatgtt cagccgcacg ggatcctggc cagcggcgat ctcggcgccc ctttcggcga
     2461 cgcgccgcag aa
//
LOCUS       DASUMG010000552         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_878089, whole genome shotgun sequence.
ACCESSION   DASUMG010000552 DASUMG010000000
VERSION     DASUMG010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878089"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VFX46_09220"
     CDS             <1..425
                     /locus_tag="VFX46_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600805.1"
                     /translation="FRNAHCTDQIAATSGAERSKSLHQQVPAWESVTIQARAADIVPP
                     PPTPAVRTAPRRASEPALCRVLMASYGGTKTMLIRYVAEDAVEYTALRVLDGFERSMT
                     ESYMSAHAPGGTLIGEFPSSDEAFARAYELCPGTDRGR"
     gene            complement(506..2251)
                     /locus_tag="VFX46_09225"
     CDS             complement(506..2251)
                     /locus_tag="VFX46_09225"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX5600806.1"
                     /translation="MSNPENGAPKEKGLLQGLQNYGDRDFSIYMRRAFMKSMGYSADE
                     LSRPVIGIAATASQFNPCHRHFPELIEAVRRGVTAAGGLALTFPTISLHESTLSPNSM
                     KFRNLMAMDTEEMIRAQPMDAVVLIGGCDKTVPAQLMGAASAGKPAIQLIGGPQMTSR
                     YGDDRVGACTDCRKFWSKFRAGEIGKAEIEAVESRLVTTAGTCAVMGTASTMAAIAEA
                     LGMTLPGTAAIPAVHADRLRAAEATGVEAVRLARSGLTPDKIITAKSVENAARVLLAL
                     GGSTNGVIHLAAIAGRLGVSLDLRRFNELSDTTPVLVAVKPVGNEFYMEDFFAAGGIG
                     AVLRELKPLLHLDCLTVTGQTLGERLAAEEGAYVDRRVIRPASDPLEPQGGLVALFGS
                     LAPDGAIIKRSAATPELFEIEGRAVVFSSLDDMAQRIDDPDLDVRSDDILVLQNAGPR
                     SPYGMPEAGFLPIPKKLLAQGVKDMVRISDARMSGTAYGTIVLHVAPEAAAGGPLGLV
                     RSGDRIRLSVKERRLDLLVSEDELARRRAEKAAQTPELPARGYDRLFRECVLQAPQGC
                     DFDFLTPQGRSSSIG"
     gene            complement(2341..>2516)
                     /locus_tag="VFX46_09230"
     CDS             complement(2341..>2516)
                     /locus_tag="VFX46_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX5600807.1"
                     /translation="HLVKAGELLGSMLLTWANVWFYQELMQAMRTAIAEGRFAQFAAE
                     HRERLARLETENE"
BASE COUNT          435 a          867 c          804 g          410 t
ORIGIN      
        1 gattccgaaa cgcccactgc actgaccaaa tcgccgccac gagcggagcc gagcgctcga
       61 aatcgctcca tcagcaggtg ccggcctggg agtcggtgac gatccaagcc cgcgccgcgg
      121 acatcgttcc gccgcccccg acaccggccg tgcgtacggc accgcggcgc gcgtcggagc
      181 ccgcgctctg tcgcgtgctg atggcgagct acggcggcac caagacgatg ttgatccgat
      241 atgtcgctga agacgccgtg gagtatacgg cactccgtgt gctggatggg ttcgagcgct
      301 cgatgaccga gagctacatg agcgcccacg cgcccggcgg caccctcatc ggcgagttcc
      361 cctccagcga cgaagccttc gcccgcgcct acgagctctg ccccggcacg gaccgtggcc
      421 gatgagcagt gctcagccct ccccatgccg gggaggggca cattcgtccg aacgcgcgat
      481 aaacgctcga gtgaacgtct caaactcagc cgatagagct tgaacgcccc tgcggcgtca
      541 ggaagtcgaa gtcgcagcct tgcggcgcct ggagaacgca ctcgcggaac aacctgtcgt
      601 agccgcgggc cggtagctcg ggcgtttgcg ccgccttctc ggcgcggcgg cgggcaagct
      661 cgtcctcaga aaccagaagg tcgaggcggc gctccttgac gctcaatcgg atgcggtccc
      721 ccgagcggac gaggcccagt ggtccgccgg ccgcagcctc gggtgccacg tggagcacga
      781 tggtgccgta ggccgtgccg gacatgcgcg catccgagat ccgcaccatg tccttgacgc
      841 cctgcgccag cagcttcttc gggatcggca agaagcccgc ctcgggcatt ccgtagggcg
      901 agcgtgggcc ggcgttctgc agcacgagga tatcgtcgga acgcacatcg aggtcgggat
      961 catcgatgcg ctgggccata tcgtccagcg agctgaagac gaccgcccgg ccctcaatct
     1021 cgaacagctc cggggtcgct gcggagcgct tgatgatggc accatcgggc gccaatgacc
     1081 cgaagagcgc gacgagcccg ccctggggct ccagcggatc gcttgcgggc cggatcaccc
     1141 gccggtcgac gtaagcgccc tcctctgccg ccagccgctc tccaagcgtt tgcccggtga
     1201 cggtcagaca atcgaggtgg agcaacggct tcaactcgcg cagcacggcg ccaatgccgc
     1261 cagcggcaaa gaagtcctcc atatagaact cgttcccgac cggcttgacg gcgacgagca
     1321 ctggcgtcgt gtcggagagc tcgttgaagc ggcgcaggtc gagcgaaaca ccgagccgcc
     1381 ctgcaatggc tgccaagtga atgacaccgt tcgtcgagcc gccgagggct agcagaacgc
     1441 gggcagcatt ctcgaccgac ttcgccgtga tgatcttgtc cggcgtcagt cccgagcggg
     1501 cgagccgcac ggcttccacg ccggtcgcct ccgccgcccg cagccggtcc gcgtgcacgg
     1561 caggaatggc cgccgtgccc ggcaaggtca tgccgagcgc ctccgcgatg gctgccatgg
     1621 tcgaagccgt ccccatcacg gcgcaggtgc ctgcggtagt gacgagacgg ctctcgaccg
     1681 cctcgatctc ggccttgccg atctcgccgg cgcgaaactt gctccagaac ttccggcagt
     1741 cggtgcaggc cccgacccga tcgtcgccat agcgcgaggt catctgcggt ccgccgatga
     1801 gctggatcgc cggcttgccc gcgctcgccg cccccatgag ctgggccggg acggtcttat
     1861 cgcagccgcc tatcagcacc acggcatcca taggctgggc acggatcatc tcctccgtgt
     1921 ccatggccat gagattgcgg aacttcatcg agttgggcga gagcgtcgac tcgtgaaggg
     1981 agatagtcgg aaacgtcagc gccaagcctc ccgctgccgt cacaccgcgc cgcacggcct
     2041 cgatcagctc cggaaagtgg cggtggcatg gattgaactg actcgcagtc gccgcgatgc
     2101 cgatgaccgg acgcgagagc tcgtccgccg agtaacccat cgacttcatg aacgcgcgcc
     2161 gcatgtagat cgagaaatcg cggtcgccgt agttctgcag gccctgcagc agccccttct
     2221 cctttggcgc accgttctcc gggttcgaca tgacgctttc ggctcccctt ctctctatct
     2281 cggctcgcgc gaccatatca ggacgcgcgg aacgcgccag tagctttgct cttgatctga
     2341 ctactcgttt tcagtttcga gacgtgccag ccgctcgcga tgctcggctg cgaactgggc
     2401 aaagcggcct tcagcgatgg ccgtgcgcat agcttgcatc agctcctggt agaaccagac
     2461 gttggcccag gtgagcaaca tcgagccgag gagctcgccc gccttgacga ggtggt
//
LOCUS       DASUMG010000553         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_798378, whole genome shotgun sequence.
ACCESSION   DASUMG010000553 DASUMG010000000
VERSION     DASUMG010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798378"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VFX46_09235"
     CDS             complement(<1..202)
                     /locus_tag="VFX46_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HEX5600808.1"
                     /translation="MRKNSSARSGPTQRQLRVGELIRQALSELLIRGEIHDEVITSHV
                     ITISEVRMSPDLRLATIFVMPLG"
     gene            complement(302..3052)
                     /gene="infB"
                     /locus_tag="VFX46_09240"
     CDS             complement(302..3052)
                     /gene="infB"
                     /locus_tag="VFX46_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX5600809.1"
                     /translation="MSETKETTEKTVLTGARKPLSLKRTVESGHVQQKFSHGRSKSVV
                     VEKKRKRTLPGQEPEETPAAIAAAQARLRQQAAQRRAAGAPQKAEEQPQPRPKTLRTL
                     SEEEQLARARALAAAQARELEEQRAREQREREEAERRRREEERKAAAELEQAAKPEAP
                     EKPESAEAAVEAAAPPTAEAPAAEAPAPEPEVSKAPETPTPPAEKERAPRTEEVPQVL
                     AQPIGMTRPIIVSRPERPKPVEAPKKAEPEAPKRAVKDEDLADLDEEEVRAKKKGGKL
                     ARAPIKGAEETRLRGKLTITNAFDERQRERSLASLKRRRERERLRAMGIPQTREKIVR
                     EVVIPEAITIQELSNRMSERAVDVIKFLMKQGEMHKITDVIDADTAELIVQEFGHTAK
                     RVSEADVEEGFIGAEDAPETLKPRPPVVTIMGHVDHGKTSLLDAIRQANVASGEAGGI
                     TQHIGAYQVKTQAGETITFIDTPGHEAFTAMRARGAKVTDIVVLVVAADDGVMPQTVE
                     AINHARAAGVPIIVAINKIDKPDADPSRVRTELLQHEIVVESMSGEVLEVEVSALKRM
                     NLDKLLEAISLQAELLELKANPDRSAEGFVIEAKLERGRGPVGTLLVQRGTLRVGDIV
                     VAGTSWGRVRALINDKGENVKEAGPSVPVEVLGFDSAPEAGDQFAVVENEARARELTE
                     YRIRKRRETLGSVGTARTLEQMMQQLKDAEKKEFPLVIKGDVQGSVEAIAGSLKKIST
                     DEVEARIIHAGVGGITESDVALAQTAGAVIIGFNVRANAQARAAAEREGVEIRYYNII
                     YDLVDDVKAAMSGLLAPTVREVFLGNAEVLEVFNITKVGKVAGCRVTEGKVERGAKVR
                     LIRDNVVVHEGTLVSLKRFKDEVKEVPAGQECGMAFANYQDIRVGDVVECYNVETIKR
                     TL"
     gene            complement(3086..>3350)
                     /locus_tag="VFX46_09245"
     CDS             complement(3086..>3350)
                     /locus_tag="VFX46_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600810.1"
                     /translation="LHGREAAADGVGKLNRRFQAVCRQKGKPALIIQELTVEQMSLAL
                     GRSNVVHAALGAGGGTTNFLNEVGRLTRYRSGKPALNIESAAG"
BASE COUNT          501 a         1159 c         1013 g          677 t
ORIGIN      
        1 cacccaacgg catgacaaaa atcgtggcga gccgtaagtc tggcgacatc cgcacctccg
       61 agatggtgat cacgtgtgag gtaatcacct cgtcgtggat ctcgccgcga atcagcagtt
      121 cggaaagtgc ctgacgaatc agctcgccaa ctctgagttg gcgctgcgtt gggcctgagc
      181 gggcagatga attcttacgc atgtttccac cgaccggact cgaaccggct cagcctccag
      241 aggacgcttc ggcgagcgcg ggacagggcc gcacccgccg agctcagctc gtttcaagcg
      301 atcaaagggt ccgcttgatg gtctcgacgt tgtagcactc gaccacgtcg ccgacgcgga
      361 tgtcctggta gttggcaaac gccataccac actcttgccc cgccgggact tccttcactt
      421 cgtccttgaa tcgcttcaag gacaccagtg ttccttcgtg caccacaacg ttgtcgcgga
      481 tcaggcggac cttggcgccg cgctccacct tgccctcggt gacacggcag ccagccacct
      541 tgccgacctt cgtgatgttg aacacctcga gaacttccgc gttgcccagg aagacctcgc
      601 gcaccgtcgg ggccagcagg ccggacatcg ccgctttcac gtcatccacg agatcataga
      661 tgatgttgta gtagcggatc tcgacgccct cccgttccgc agccgcccga gcttgtgcgt
      721 tggcgcgcac gttgaatccg atgatgacgg cgccggccgt ctgcgccagg gcgacgtccg
      781 attcggtgat gccgccaacg cccgcatgga tgatgcgcgc ctcgacttca tcggtcgaga
      841 tcttcttcaa ggagccggcg attgcttcaa cggagccctg cacgtcaccc ttgatgacca
      901 gcgggaactc cttcttctcc gcgtccttga gctgctgcat catctgctcg agggtgcggg
      961 cggtgccaac cgaccccagc gtctcgcgac gcttgcgtat gcggtactcg gtgagctcgc
     1021 gggcacgagc ctcgttctcg acaacagcga actggtcgcc tgcttcggga gccgagtcga
     1081 agcccaggac ctcgacgggc accgaggggc cggcctcctt cacgttctcg cccttgtcgt
     1141 tgatcagtgc gcgcacgcgg ccccaggagg tgcccgcgac gacgatgtcg ccgacgcgca
     1201 gcgtgccacg ctggacgagc agcgtgccaa ccgggccacg gccgcgctct agcttcgcct
     1261 cgatgacgaa gccttccgcg gagcgatccg gattggcctt aagctcgagc agctcagcct
     1321 gcagcgagat ggcctcgagc agcttgtcga ggttcatgcg cttcaaggcc gatacttcga
     1381 cctcgagcac ctcacccgac atgctttcga ccacgatttc gtgctgcagc aactccgtgc
     1441 ggacccggct gggatcggca tccggcttgt cgatcttgtt gatcgccacg atgatcggca
     1501 cgccagccgc gcgtgcgtga ttgatggcct cgaccgtttg cggcatgacg ccgtcatcgg
     1561 ctgcgaccac caacacgaca atgtccgtta ccttggcgcc acgggcgcgc atggcggtga
     1621 acgcctcatg gcccggcgta tcgatgaagg tgatcgtctc gccggcctgc gtcttcacct
     1681 ggtacgcgcc gatgtgctgg gtgatgccgc ccgcctcgcc gctagcaacg ttcgcctgcc
     1741 gaatggcatc cagcagcgaa gtcttgccgt ggtcgacgtg acccataatg gtgaccacgg
     1801 gcggacgggg cttgagcgtc tcgggcgcgt cctcggcgcc gatgaagcct tcctcgacgt
     1861 cagcttcgga aactcgcttc gccgtgtgcc cgaattcctg cacgatgagc tcggccgtat
     1921 cggcgtcgat cacgtccgtg atcttgtgca tctcgccctg cttcatcagg aacttgatga
     1981 cgtcgacggc gcgctccgac atgcggttag agagctcctg gatcgtgatc gcctcgggga
     2041 tgaccacctc gcgcacgatc ttctcgcgcg tctgggggat acccatcgcg cgcagccttt
     2101 cgcgctcgcg gcgacgcttc agcgaggcaa gtgaccgctc ccgctggcgc tcgtcgaaag
     2161 cgttcgtgat cgtcagcttg ccgcgcaggc gagtctcttc agcacccttg atgggagccc
     2221 gggcgagctt gccgcccttc ttcttggcgc ggacctcctc ctcgtcgaga tctgcaagat
     2281 cctcgtcctt aacggcacgc ttgggagctt cgggctcagc tttcttgggt gcttcgacgg
     2341 gcttcggccg ctccggacgc gacacgatga tcggccgcgt catgccgatg ggctgtgcca
     2401 agacctgcgg aacctcttcg gtccgcggcg ctctctcctt ttcggcaggg ggagtcggcg
     2461 tttcgggagc cttgctgacc tccggttcag gcgcaggcgc ctctgcagcc ggggcctcag
     2521 ccgtcggcgg cgcagcagcc tctacggcgg cctcggcgct ctccggcttt tcgggcgcct
     2581 ccggcttggc agcctgctcc agctcggcag cggccttgcg ctcctcctcg cgacggcggc
     2641 gctccgcctc ctcgcgctcg cgctgctcgc gagcacgctg ctcctcgagc tcacgcgcct
     2701 gcgctgcggc gagcgccctc gcgcgcgcaa gctgctcctc ctcggagagc gtgcgcagag
     2761 ttttcggccg gggctgcggc tgctcctcag ccttctgagg cgctcctgca gcacgccgct
     2821 gggcggcctg ttggcgcagg cgtgcctgag cggcggcgat cgcagccgga gtttcctccg
     2881 gctcctgccc cgggagtgtc cgcttcctct ttttctcgac caccaccgat ttcgaccggc
     2941 cgtggctgaa cttctgctgc acgtgcccgg actcgacggt gcgcttcagg ctgagcggct
     3001 tgcgcgcgcc tgtcagcacg gtcttctcag tggtctcttt tgtttcgctc atgccttgcc
     3061 tgtccaaacg tcccctgtgc gaccgtcacc cggccgccga ttcgatgttc aaagccggct
     3121 tgcccgaccg gtatcgggtc aatcggccaa cttcgttcag gaaattcgtc gtaccgccgc
     3181 ctgcgcccag tgcagcatgt accacattcg accgtcctaa ggccaagctc atttgctcga
     3241 cggtcaactc ctgaatgatc aacgccggtt ttcccttctg ccggcaaacc gcttgaaaac
     3301 ggcgattcaa tttaccgacg ccatctgccg cggcctcgcg gccgtgcagc
//
LOCUS       DASUMG010000554         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_717500, whole genome shotgun sequence.
ACCESSION   DASUMG010000554 DASUMG010000000
VERSION     DASUMG010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717500"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /locus_tag="VFX46_09250"
     CDS             complement(<1..1165)
                     /locus_tag="VFX46_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX5600811.1"
                     /translation="MQERVQNTEAAEFKIIGTRPIRPDGVDKVTGRARFGADYNLPGQ
                     LIGKVLRSPYPHARIKRIDTSKAEKLPGVKAIVTSADFPDQPSEFVPAGELLVNYRDL
                     VRNIMAREKVLYEGHAVAAVAATSASIAKQALKLIEVEYEVLPHVIDVDEAMQPNAPL
                     LHEDLITSGVSPAPNTPSNIAKRVEFGRGDLEKGFAEADVIIEREFKTKPVHQGYIEP
                     HACHANVSEDGQAELWVTTQGHWVVRAHCARLLGWDVSKIRVTATEIGGGFGGKTVVY
                     LEPVALALAAKAKRPVKMVMTRDEVFRATGPTSGASMKVKIGAKRDGRITAAEALLKY
                     QAGAFQGSPIGPGAMSAFAPYDLESVKTVGYDVVTNRPKVAAYRAPGAPISEFA"
     gene            complement(1169..1645)
                     /locus_tag="VFX46_09255"
     CDS             complement(1169..1645)
                     /locus_tag="VFX46_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX5600812.1"
                     /translation="MAKHHIAFTVNGDPVEVLCETQQTLLDVLRDELGLTGTKEGCGT
                     GDCGACSVVVDGRLVCSCLMLGVEANGKSVETIEGMGRGEELHPLQRKFLEHAALQCG
                     ICTPGFLVAAKALLDRNPNPTETEVRYWLAGNLCRCTGYDKIVRAVMDAAAEMRRA"
     gene            complement(1647..2507)
                     /locus_tag="VFX46_09260"
     CDS             complement(1647..2507)
                     /locus_tag="VFX46_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX5600813.1"
                     /translation="MRYEAPETLEAAIAILRSEPGEARVLAGGTDLLVQLKTDLIEPQ
                     LVVDIKKIRELREVRREAEGYRIGAAVTGAELKEHPELKAVWPGVIEAANLIGSTQIQ
                     GRATLGGNLCNGSPAADSVPALIAAGATVTVAGPNGRRDVPVEDIVLGPRKLSIAKDE
                     IVVSFLLPPRPPRAADAYLRFIPRTEMDIAVVGAGVNLTLGEDGRVTAARVGLGAVAA
                     RPLLVPAAAEALIGSALEATALAKFEEAVRAACQPIDDKRGTREFRIKVAGVMARRAA
                     QKAYERARSR"
BASE COUNT          501 a          974 c          881 g          555 t
ORIGIN      
        1 cggcaaactc ggaaatcgga gcgccggggg cacggtaagc agcgaccttg ggtcggttcg
       61 tcaccacgtc gtagcccacg gtcttgacgc tctcgaggtc atatggcgcg aaggcgctca
      121 tcgccccagg cccgatgggc gagccttgga aagccccggc ctggtacttg aggagcgcct
      181 cggcagcagt gatccggccg tccctcttcg ccccgatctt gaccttcatg ctggcgcccg
      241 aggtcgggcc ggtcgcgcgg aagacttcgt cccgcgtcat caccatcttc accggccgct
      301 ttgcctttgc agccagcgcc aaggccactg gttcgagata gaccaccgtc ttgccgccga
      361 acccgccgcc aatctcggtc gctgtcacgc gaattttcga gacatcccag ccgagcagcc
      421 gggcgcagtg ggcgcgcaca acccaatgac cttgcgtcgt cacccacagc tccgcctgtc
      481 cgtcttcgga gacattggcg tggcaagcgt gaggctcgat gtagccttga tgaacgggtt
      541 tggtcttgaa ctcccgctcg atgatgacgt cggcctcggc gaagcccttc tccaagtcgc
      601 cgcgcccgaa ctccactcgc ttggcgatgt tggagggcgt gttgggagcc gggctgacgc
      661 ctgaagtgat caaatcctca tgcagcagcg gtgcgttcgg ctgcatggcc tcatcgacgt
      721 cgatgacgtg cggcaagacc tcgtattcga cttcgatgag ttttagggcc tgcttcgcaa
      781 tgctcgcact ggtggccgca acagcggcga ctgcgtggcc ttcgtagagc accttctcgc
      841 gcgccatgat gttgcgcacg agatcgcggt aattgaccag cagctcacct gcgggcacga
      901 attccgatgg ctgatcggga aagtccgcac tggtgacaat ggccttcacg cccggcagct
      961 tttcggcctt ggaggtgtcg atgcgcttga tgcgtgcgtg cggatacggg ctgcgcagca
     1021 cctttccgat cagctgaccg gggaggttgt aatcggcgcc gaaccgggcc cggccagtca
     1081 ctttgtccac accgtccggg cgaataggac gcgtaccgat gatcttgaac tcggcggcct
     1141 cggtattctg gacacgttcc tgcatggctc aggccctcct catctcggcc gcagcatcca
     1201 tcacggcacg cacgatcttg tcgtagccgg tgcaccggca gaggttgccg gccagccaat
     1261 agcgcacctc ggtttcggtg gggttcggat tgcggtccag cagcgccttt gcggcgacga
     1321 ggaacccagg cgtgcagatg ccgcactgga gcgcggcgtg ttcgagaaac ttgcgctgca
     1381 gcggatgcaa ctcctcgccg cggcccatgc cttcgatggt ctcaaccgat ttgccgttgg
     1441 cttcgacccc aagcatcagg cacgagcata cgagtcggcc atcgaccacg acgctgcagg
     1501 caccgcagtc gcccgtcccg caaccctcct tggtgcccgt tagcccgagc tcgtcgcgca
     1561 ggacatcgag cagggtctgc tgcgtttcgc agagaacctc gacggggtcg ccattcaccg
     1621 tgaacgcaat atgatgtttc gccatcttat cgagacctcg cccgctcata ggctttctgc
     1681 gctgcccggc gcgccatcac gccggcaacc ttgatccgaa actcgcgcgt gcctcgcttg
     1741 tcatcgatcg gctgacacgc cgcacggacc gcctcttcaa acttggccaa tgccgtagcc
     1801 tcaagcgcgc tgccgatcag cgcctccgcc gcggcaggca cgagcagagg ccgtgccgcg
     1861 accgcaccca gcccgacacg cgccgccgta actctaccgt cctcacccag cgtcaggttc
     1921 acgcctgcgc cgacgacagc gatgtccatc tccgttcgcg gaatgaagcg caggtaggca
     1981 tctgccgctc gcggcggtcg tggcggaagc aggaatgaaa cgacgatttc gtctttagcg
     2041 atcgatagct tgcggggtcc gagcacgatg tcttccaccg ggacatcgcg gcgtccattc
     2101 gggcctgcga ccgtaacagt cgcaccggca gcaatgagcg cgggcacgct gtctgcggcc
     2161 ggcgagccgt tgcagagatt gcctccgagc gtcgcgcgcc cctggatctg ggtcgagccg
     2221 atgagattgg ccgcctcgat gacgccgggc cacacggcct tgagctccgg gtgctctttg
     2281 agctcggcgc cggtaacggc tgcaccgatc ctgtagccct cggcctcgcg ccggacctcg
     2341 cgtagctcgc ggatcttctt gatgtcgacc acgagctgcg gctcgatgag atcggtcttg
     2401 agctggacga gtagatcagt tcctccggcg agaacgcggg cctcaccggg ttcggaacgc
     2461 aggatcgcaa ttgccgcctc aagcgtttcc ggcgcctcgt aacgcatgca tgccctctac
     2521 agtcgctaag cgctgggttt cgggagctag gaaatcacaa tcgcgggcat tgcgccaaca
     2581 gctcatgccc cttttgaact ctctcccaac acgtgctgcg cgtcttcccc tcgcacgtat
     2641 gcggacgatg gtgtagcaat cgaggagcga ccgcatacac cgtcgcaaga ccccaaggag
     2701 gagcgtcatt attcatggga gacaccgcca tgcccgttag ccgccgaact tttcttatga
     2761 cagctggagc cagcattgct gctcccgccg tcctgcgcgt gacgagggcc aatgccgccg
     2821 aggtaaccct gcggctacat cattttctcc ctcctgtcgc aagcgtcccc aagcacatgc
     2881 tcgagccgtg ggccaagcgc attgaagacg g
//
LOCUS       DASUMG010000555         4266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_637360, whole genome shotgun sequence.
ACCESSION   DASUMG010000555 DASUMG010000000
VERSION     DASUMG010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637360"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VFX46_09265"
     CDS             complement(<1..528)
                     /locus_tag="VFX46_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX5600814.1"
                     /translation="MHVGVIGTGYVGLVVGACLAETGNDVICADIDEEKIARLSRGEI
                     PIYEPGLEPLVRRNLEEGRLRFTTDVGEAVRQSEVIFIAVGTPEGEDGSADLRHVLSV
                     AEAIGRNMNGEKIVITKSTVPVGTAKRVRAVIEEHTSHPVHVCSNPEFLKEGAAVEDF
                     MKPDRVVIGVESEYAK"
     gene            complement(649..1767)
                     /locus_tag="VFX46_09270"
     CDS             complement(649..1767)
                     /locus_tag="VFX46_09270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX5600815.1"
                     /translation="MPVPLLDLVSQYDTIRSEVDAAVAEVVRSQRFILGPTVERFEAA
                     IAEYIGVRHAIGCASGTDAILLSLRALDIEPGAEVIVPAFTFFATAGAVWNAGLTPVF
                     VDIDPVSYNVDPESVRRAVTSRTRAIIPVHLFGQMADMDAIMAVAAEHGLHVVEDAAQ
                     AIGAMQRIGGELRAAGTVGATGAFSFFPTKNLGGFGDGGLVTTNDDGLADRLRKLRVH
                     GGRQMYHHEMVGYNSRLDAIQAAVLSAKLPHLDRWTRLRREHASFYEKRLARLEPCVR
                     VPRTMERNEHVFNQYTLWVERRDELKRFLDDHGIGNSIYYPVPLHLQECFRSLGYRPG
                     DLPVSEAAAQHVISIPVFPELSEEQREEVAARIEEFYR"
     gene            2057..3640
                     /locus_tag="VFX46_09275"
     CDS             2057..3640
                     /locus_tag="VFX46_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HEX5600816.1"
                     /translation="MSSRREFMKHFAGAPLGAALAITGSSNVPSFLGPTDTPPVKAWI
                     DVREFGARGDGRADDSQPIQAAIDSLQGRRGIIFFPAGTYRCEAPINVKRAQLLGSGV
                     PGATGGGGTTLRCLADRGLFSSVEDNYGFEVGALRIVGSGRRQQNGQVLVDFTGQNYP
                     RMRDCRISQGEIGMRLSNGERVECHYGSFFNVHFDRCYIGVDIPGTAHSHKFFAGRFW
                     DCVYAVRNVAAGDIAFFGTEFESDYPLWHPVHARQASPITLFVGTRQESDHPPLIETG
                     SFTDVGSYWSGYRRPPAFGVRDGQVVVGGQVITAGIRSDTHPVKQNLLRNPGLIPDAD
                     EESIPGWQWRGRAPAFLMGPFGRVVRFELDSPEHALVQEGIRLPAGRYLVGFGFHTEG
                     NAEASIVVESNGRIILDARNIPANYQGGLVQRPFTLDRESDDVRVSFVRRGASGAVIY
                     YYAPFLVPTPGGEAFLPIAETGRPNEVRYGTAPPRKGAWQVGDIVYNLAPRPGGYIGW
                     TCVEAGSPGTWKGFGRIES"
     gene            complement(3695..>4266)
                     /locus_tag="VFX46_09280"
     CDS             complement(3695..>4266)
                     /locus_tag="VFX46_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HEX5600817.1"
                     /translation="RLLELIDDFNRRKQQSQARAEREFVEARLADAQRELEEAEGRLS
                     EFLSRNRRIDGSPELNFEMLSLERRVEVKQQIYVSLARSYEMARIAEVRESPVITVVE
                     PPEGSATPVGSGLVLNVAIALVFGGFIGVLFAFTQEHFDRQRRLRAADVQEFEELTRA
                     VVSELSPRRLARILRSLRPRAGVGGGGGS"
BASE COUNT          751 a         1426 c         1305 g          784 t
ORIGIN      
        1 cttcgcatac tcgctctcca cgccgatcac cacccggtcc ggcttcatga agtcctcgac
       61 cgctgccccc tccttcaaga actccggatt gctgcacaca tgcaccggat ggctcgtgtg
      121 ctcctcgatc accgcccgta cgcgcttggc cgtgcccacc ggcaccgtgc tcttcgtgat
      181 gacgatcttc tcgccgttca tgttgcgccc gatcgcctcg gccacagaaa gcacgtgcct
      241 caggtccgca ctgccgtcct cgccctccgg cgtgcccacc gcaatgaaga tcacctcgct
      301 ctgccggacc gcctctccca catccgtcgt gaagcggagc cgtccctcct ccaggttgcg
      361 gcgcaccagc ggctcgagcc ccggctcgta gatcggtatc tcgccccgtg agaggcgagc
      421 gatcttctct tcgtcgatgt ccgcgcagat cacgtcattg cccgtctccg caagacacgc
      481 ccccaccacg agtcccacgt accccgttcc gatgacacct acgtgcatgg agcctcccac
      541 caatcgaggt cctacgcgcc cggctcggac gaacgttccg gaccggccga cgttgcaatg
      601 atagtaccgg gtcggtctcc catcgaccga gaacccgtgt cgtttcgctc agcgatagaa
      661 ctcttcaatg cgcgctgcca cttcttcccg ctgctcttcc gacagctccg ggaacactgg
      721 gatactgatg acgtgttgcg ccgcggcctc actcaccggc aggtcgcccg ggcggtaccc
      781 gagggaacga aagcactcct gcaagtggag tggcacagga tagtagatcg agttcccaat
      841 cccgtggtcg tcgaggaatc gtttcaactc gtcccgccgc tccacccaga gagtgtattg
      901 attgaacacg tgctcgttac gctccatcgt gcggggtacc cgaacacacg gctccagacg
      961 cgcgagccgc ttttcataga aagaggcgtg ttcgcggcgg agacgggtcc agcggtcgag
     1021 atgcggtagc ttggccgaaa gtacggcagc ctggatcgcg tccaggcgcg agttgtatcc
     1081 caccatctcg tgatggtaca tttgccgtcc cccgtggaca cgaagcttcc tgaggcggtc
     1141 cgcaagccca tcgtcgttgg tcgtcacgag tccgccatct ccgaatcctc cgagattctt
     1201 tgtggggaag aaagagaaag cgccggtcgc gcccacggtg ccggctgccc gcagctcgcc
     1261 accgatccgt tgcatggccc cgatcgcctg ggcggcatcc tccacgacgt gcaatccgtg
     1321 ctcggctgcg acagccataa tggcatccat gtcggccatc tggccaaaga ggtgcaccgg
     1381 gattatcgcc cgagtccgag atgtcaccgc ccgccgtacc gattccggat cgacgttgta
     1441 gctcaccgga tcgatgtcca cgaatacggg cgtgagtcca gcgttccaca ccgcgccggc
     1501 cgtggcgaag aatgtgaatg ctgggacgat cacttccgcg ccgggctcga tgtccagcgc
     1561 tctgagcgaa aggaggatcg cgtcggttcc gctcgcgcat ccgatggcgt ggcgaacccc
     1621 aatgtattcg gctatcgcag cctcgaaccg ctcgacggtg ggcccgagga tgaaccgctg
     1681 cgaccggacg acttcggcaa cggcggcgtc cacctctgag cggatcgtat cgtattgcga
     1741 aacgaggtcc aggagaggaa caggcatacc tacttccaga gacggaaaac gaggagtttg
     1801 gacgttggcg cactttccgc gcgaaccggg cggtgaaagt acagccttcc gtgcaccccg
     1861 gccaccccga agagacgcgc tcgggaggtg ggtcggggct cgactcgtac ggttcgtggc
     1921 tgacgtcggg ttgggcgcgg agcgttggat tggaagctcg gggctccggt gctatgttcc
     1981 cccgacccct tgggttttcg ctatcccgga cttttcaacg cgagttgagg acctgagaga
     2041 cggtgggggt tcggtcatgt cgagtcgccg tgagttcatg aagcacttcg cgggcgcccc
     2101 actgggtgcc gcccttgcca taacgggcag ctccaatgtg ccctcgttcc tggggccgac
     2161 ggacacaccc ccggtcaagg cgtggatcga tgtccgagag ttcggtgccc gaggcgacgg
     2221 ccgtgccgat gattcgcaac ccatccaggc agcgatcgat tcgctccagg gccgtcgtgg
     2281 catcatcttt ttccctgcgg gtacgtatcg ctgcgaggct ccaatcaatg taaagcgagc
     2341 acaactgctc ggcagcggag tacccggagc cactggaggc ggcggcacga ctctccggtg
     2401 cctcgccgac cgcggcctgt tctcgagtgt ggaggacaac tacggcttcg aagtcggagc
     2461 gttgcgcatc gtcggatcgg gccggcgcca acagaacggg caggtgctcg tcgatttcac
     2521 cggccagaat tatccgagga tgcgcgattg ccggatctcc caaggggaga tcggtatgcg
     2581 tctcagcaac ggcgagcgcg tagagtgtca ctacggctca ttcttcaacg tgcacttcga
     2641 ccgttgctac atcggggtcg acatcccggg caccgcgcac tcgcacaaat tcttcgcggg
     2701 ccgtttctgg gactgcgtgt acgccgtgcg caatgtcgca gccggcgaca tcgcgttctt
     2761 cgggacggaa ttcgaatccg actacccgct ctggcatccg gtccacgccc ggcaggcctc
     2821 tccgatcacg ctcttcgtcg gtacccgcca ggaaagcgat cacccgccgc tcatcgagac
     2881 cggctcgttc accgacgtcg gctcgtactg gagcgggtat cgcaggcctc cggccttcgg
     2941 cgttcgcgac ggacaggtag tggtcggcgg acaggtgatc acggccggga tccgctcgga
     3001 cacacacccg gtcaagcaga acctgctacg caatccaggt ctgatcccgg acgccgacga
     3061 ggaatccatc cccggttggc agtggcgagg acgagcgccg gcgttcctca tggggccatt
     3121 cggccgcgtg gtgcgtttcg agttggactc gcccgaacac gcactcgtgc aggaggggat
     3181 ccggctcccg gcaggtcgct acctggtcgg cttcggtttc cataccgaag gcaacgccga
     3241 agcgagcatt gtcgtggaat cgaatggcag gatcattctc gatgcccgca acatcccggc
     3301 gaactaccaa ggaggactcg ttcaacgccc gttcacgctc gatcgagaaa gcgacgacgt
     3361 acgcgtgtcg ttcgtccgga gaggcgcatc gggcgccgtc atctactact acgcaccctt
     3421 cttggtcccg acgcctggcg gcgaggcgtt cctgccgatc gccgagaccg gtcgcccgaa
     3481 cgaagtacga tacggcacgg caccccctcg caaaggggcg tggcaagtcg gcgacattgt
     3541 ttacaatctt gcaccccgac ccggcgggta tatcgggtgg acgtgcgttg aagccggctc
     3601 accggggacg tggaagggat tcggccgcat cgaatcgtga gtccggcgcc gggcggggcg
     3661 cggtgggccc tggatcagag tgggccaacc ctgctcatga tcccccaccc ccgccgactc
     3721 cggcgcgggg acgcagggac cggagaatcc gggccagcct gcgtggagag agctcgctca
     3781 cgaccgcccg cgtcagttcc tcgaactcct gaacatctgc ggcccgaagt cgccgttgtc
     3841 ggtcgaagtg ctcctgcgtg aaggcgaaga ggacgccgat gaatccgccg aaaacgagcg
     3901 cgatcgcgac gttcagcacg aggccactcc ctaccggggt agccgagccc tctggaggct
     3961 cgaccaccgt gatgacggga ctctcgcgca cttcggcgat gcgcgccatc tcgtatgatc
     4021 tggcgaggga gacgtaaatc tgctgtttca cctcgactcg ccgctccaac gagagcatct
     4081 cgaaattcag ttcgggcgag ccgtcgatcc gccggtttcg gctcaggaat tcgctcaacc
     4141 tcccctcggc ctcctcgagc tcccgctgcg catctgccaa ccgcgcctcg acgaactccc
     4201 gctctgcacg cgcctgtgat tgctgcttgc ggcggttgaa gtcatcgatc agctcgagga
     4261 gcctcc
//
LOCUS       DASUMG010000556         3826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_476895, whole genome shotgun sequence.
ACCESSION   DASUMG010000556 DASUMG010000000
VERSION     DASUMG010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476895"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VFX46_09285"
     CDS             complement(<1..1290)
                     /locus_tag="VFX46_09285"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEX5600818.1"
                     /translation="MPTQVLMPALSPTMEEGKLAKWLVKEGDEVKSGMVIAEIETDKA
                     TMEVEAVDEGRIGRILVPEGTEGVKVNTPIAVIFQDGEDSGAPIPGSPEAARDVKQAD
                     SQSLPAQARPAGDGVSRSPDLPSPAVAPSREVAPEIDLPPGTEMITQTMREALRDAMA
                     EEMRRDPDVFLMGEEVGQYQGAYKVSQGLLDEFGEKRVIDTPITEQGFAGIGVGAAFA
                     GLKPIVEFMTWNFAMQAIDQIVNSAAKTLYMSGGQMGCSIVFRGPNGAAARVAAQHSQ
                     EYSAWYAHVPGLKVVAPSNPADAKGLLKSAIRDPNPIIFLENEILYGTSGPVPKIDDW
                     LVPIGKARIARPGKDVTIVSWSRGMTYALDAAERLAKEGIEAEVIDLRTLRPLDLDTV
                     LESVRKTNRIVTVEESWPVCSVGSEICAQVAIQAFDYL"
     gene            complement(1414..2436)
                     /gene="pdhA"
                     /locus_tag="VFX46_09290"
     CDS             complement(1414..2436)
                     /gene="pdhA"
                     /locus_tag="VFX46_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX5600819.1"
                     /translation="MPANGADSVVAEQERTEADVPELTREQELHAYREMLLIRRFEEK
                     AGQLYGMGFIGGFCHLYIGQEAVVVGLQMCAQEGDQVITTYRDHGHMLACGMDPKRVM
                     AELTGRRSGYSKGKGGSMHMFSVERNFYGGHGIVGANVPLGAGLAFANKYRGNNNITF
                     CYFGDGAANQGQVYETFNMSKLWKLPVLFIIENNKYAMGTSVERSAATTDLSQRGRSF
                     DIPGERVDGMDVRAVKAAGDKAAKWVREGNGPYILEMLTYRYRGHSMSDPAKYRTREE
                     VQRVREERDPIERVRARLLEKKQATEEELKAIDAEIRRIVNEAAEFAQTDPEPDPSEL
                     WTDVLR"
     gene            complement(2616..2936)
                     /locus_tag="VFX46_09295"
     CDS             complement(2616..2936)
                     /locus_tag="VFX46_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX5600820.1"
                     /translation="MRRIRQHLLVLLGCLSVSAYFIHHTITGKHGLEARSQLHERSRA
                     LDREIADLEAVAAELLRDVLLLRPDPPHPDMVEEIAIGVLGMARPGDRIVLVGQGVGR
                     EAVP"
     gene            complement(3058..>3826)
                     /locus_tag="VFX46_09300"
     CDS             complement(3058..>3826)
                     /locus_tag="VFX46_09300"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX5600821.1"
                     /translation="TCADAIRMGAEIFHTLRKALKNAGHNTNVGDEGGFAPNLQSADE
                     ALGFIMRAIEQAGYKPGDDVVLALDCASTEFFKDGKYNLEGEGKVLDSEGMARYLADL
                     VSRYPVVSIEDGMAEDDWQGWKLLTSELGDRCQLVGDDLFVTNTKRLAKGIAEGVGNS
                     ILVKVNQIGTLTETLDAVAMAQRAAYTAVISHRSGETEDATIADLAVATNAGQIKTGS
                     LSRSDRLAKYNQLIRIEEDLGDVARYAGRSVLRGATA"
BASE COUNT          677 a         1232 c         1152 g          765 t
ORIGIN      
        1 gagatagtcg aaggcttgaa tagcaacctg agcgcagatt tccgagccca cggagcatac
       61 gggccaggac tcttcaaccg tgacgatccg attcgtcttg cgcacggatt cgagaaccgt
      121 atcgagatcg agcggacgca gcgtgcgtag atcgatcacc tccgcctcga taccttcctt
      181 cgccagccgt tcggcggcat ccagggcata ggtcatgccg cgcgaccagg agacgatcgt
      241 gacgtcctta cccggccggg caatgcgcgc cttgccgatc ggtacgagcc agtcgtcgat
      301 tttcggtact ggcccagagg tcccgtaaag gatctcgttt tcgaggaaaa taatggggtt
      361 gggatcgcga atagcgctct tgagcagccc cttggcgtcg gcagggtttg atggcgcgac
      421 gaccttcagc cctggcacgt gggcgtacca agccgagtat tcctggctat gctgggcagc
      481 cacccgcgcg gcggctccgt tcggaccgcg gaacacgatg gagcagccca tctgcccgcc
      541 ggacatgtaa agggttttgg ccgcagagtt cacgatctgg tcgatcgctt gcatcgcgaa
      601 gttccaggtc atgaactcga cgatcggctt gaggccggcg aaggccgcac ccacaccgat
      661 accggcgaat ccctgctccg tgatcggcgt atcgatcacc cgtttctcgc cgaattcgtc
      721 gagcagcccc tggctcacct tataggcgcc ctggtactgc ccaacctcct cgcccatcag
      781 gaatacgtcc gggtcgcggc gcatctcctc ggccatggcg tcgcgcaaag cttcgcgcat
      841 ggtctgggtg atcatctccg tgccgggggg aaggtcgatc tccggggcca cctcgcgcga
      901 tggcgctact gctggcgagg gcaggtcggg actgcggctc acaccatcgc cagccggccg
      961 tgcctgggcc ggaaggctct ggctgtcagc ctgcttaacg tcgcgagcag cttctggcga
     1021 accaggtatc ggcgcgcccg aatcctcgcc gtcctgaaaa ataactgcga tcggcgtgtt
     1081 cactttcacg ccttcggtgc cctccggaac aagaattcgt ccgatccggc cctcgtccac
     1141 cgcctccacc tccatggtcg ccttgtccgt ctcgatctcg gcgatgacca tgccggattt
     1201 gacctcgtca ccttctttga cgagccattt ggcgagcttg ccctcctcca tggtcgggct
     1261 tagcgcgggc atcaatactt gagtgggcat ttcgactgga tctctgactg cacgctcgat
     1321 ggcctcccct cccaggctcg ggagtgcgag gatcagcgca aaaagtagtt agctacaaac
     1381 gcggttacag ctaaggctcg ggcgcccatc gccttacctc aagacgtctg tccacagctc
     1441 agatggatcc ggctcgggat cggtctgggc gaattcagca gcttcgttga cgatgcggcg
     1501 gatctcggca tcgatcgcct tcagctcctc ctccgtcgcc tgctttttct cgagcaggcg
     1561 ggcacgcaca cgctcgatgg gatcacgctc ctcgcgaact cgttggacct cctcgcgtgt
     1621 acggtacttg gctggatcgg acatcgagtg gccgcgatac cgataggtca acatctcgag
     1681 gatgtacggc ccgttgccct cgcgcaccca tttggccgcc ttgtccccgg ctgccttgac
     1741 ggcgcgcaca tccatgccgt ccacccgttc gccagggatg tcgaacgagc ggccgcgctg
     1801 cgataaatcc gtggtcgccg ccgagcgctc gaccgaggtg cccatcgcgt atttgttgtt
     1861 ctcgatgatg aacagcaccg gcagcttcca gagcttggac atgttgaagg tctcgtagac
     1921 ctggccctgg ttggcggcgc catcgccgaa gtagcagaaa gtaatattgt tgttgccgcg
     1981 gtatttgttg gcgaaggcga gccctgcgcc gagcgggacg ttggcgccca caatgccgtg
     2041 tccaccgtag aaattcctct ctacggagaa catgtgcatc gagccgccct tgcccttcga
     2101 gtaaccgctc cggcgacccg tcagctcggc catgacacgc ttgggatcca tgccgcaagc
     2161 caacatgtgg ccatggtcgc ggtaggtcgt gatgacctgg tccccctcct gggcgcacat
     2221 ctgcaggccg acgacgaccg cctcctggcc gatgtagaga tggcagaagc caccgatgaa
     2281 gcccatgccg taaagctgac cggccttttc ctcgaatcgc cggatcagta gcatttcgcg
     2341 gtaggcgtgg agctcctgct cacgggtaag ctcgggcaca tcggcctctg tgcgctcctg
     2401 ctccgctaca acggagtcgg cgccgttggc aggcatggcc aacttcgcct gttttccgcc
     2461 agatgacccc cgcgcccttt ccgcaggtgc catggatcct cctgagcctt gaaattcaat
     2521 gcggatacga tagtccgcgc caaacctaca cgagcacacc tggctttgcc agatgttggc
     2581 ccggctggga agccctaggg tgccgcatcc aggatttaag gcacagcttc ccgaccaacg
     2641 ccttgaccga ccagaacgat ccggtcaccg ggccgcgcca tcccaaggac gccgatggcg
     2701 atctcctcga ccatatcggg atgaggtgga tcgggccgaa gaaggagcac gtcgcgaagc
     2761 agctcggccg cgacggcctc gaggtcagcg atctcccggt ctagagcgcg ggaacgctcg
     2821 tggagctggg accgtgcctc gaggccgtgc ttgcccgtga tcgtgtgatg gatgaagtag
     2881 gcagatacag ataggcagcc aagcagtacc aggagatgct gccgtatcct cctcatccct
     2941 cgatccggtg atgtgcgacg cagaaccatc gagacgtgct cattcgcaac gctcgccttt
     3001 tagtccgggc gccgttaagg gcgggtaaag ccatgctccg cttgctgtgt agcggcctca
     3061 ggccgttgcg cccctcagca cgctgcggcc ggcgtagcga gcgacgtcgc ccaggtcctc
     3121 ctcgatgcgg atgagctggt tgtattttgc gaggcggtcg gaacggctca gcgatccggt
     3181 cttgatctgg ccggcgttcg tggcgacggc caagtcggca atggtggcgt cctccgtctc
     3241 gccggagcgg tgggaaatga cggccgtgta ggccgcacgc tgggccatag cgaccgcatc
     3301 cagtgtttcc gtcagtgtgc cgatctggtt gaccttgacc agaatggagt taccgacgcc
     3361 ctcggcgatg cctttcgcca gccgcttcgt gttcgtgacg aagaggtcgt ccccaacgag
     3421 ctggcagcga tcacccaatt ctgatgtgag cagcttccaa ccctgccagt catcctcggc
     3481 catgccgtcc tcgattgaca cgaccggata gcggctgacc agatcggcga ggtaacgagc
     3541 catgccttcg gaatcgagca ccttgccctc accttccagg ttgtacttcc cgtccttgaa
     3601 aaactcggta gacgcacagt cgagagccag cacgacatcg tcgcccggct tgtagcctgc
     3661 ctgctcgatg gcccgcatga tgaagccaag ggcctcgtcc gccgactgca ggttcggcgc
     3721 aaagcccccc tcgtcaccaa cgttcgtatt gtggccagcg tttttcaggg ccttgcggag
     3781 cgtatggaag atctcggcgc ccatgcggat tgcgtcggcg caggtc
//
LOCUS       DASUMG010000557         4956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_798917, whole genome shotgun sequence.
ACCESSION   DASUMG010000557 DASUMG010000000
VERSION     DASUMG010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798917"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFX46_09305"
     CDS             complement(<1..592)
                     /locus_tag="VFX46_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HEX5600822.1"
                     /translation="MPLARIAIVYHSGWGHTARLAQAVTDGVEEVEGAEALLMTVEEA
                     QTRWDDLASADAIIFGSPTYMGNVSAAFKAFQEATSKAVMAKGFAWKNKIAAGFTNSG
                     ARSGDKLATLVQIALFAAQHGMHWVNLDLPPANNSTKGSEEDLNRLGFWLGAAAQSNT
                     DQGPDLAPPESDLLTARHLGRRVAQVTLQFVRGRMVE"
     gene            complement(611..1048)
                     /locus_tag="VFX46_09310"
     CDS             complement(611..1048)
                     /locus_tag="VFX46_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX5600823.1"
                     /translation="MSKCEATRSLVFERQMKHPPEKVWRALTESHLIAEWLMENDFVP
                     KVGHRFTMRAVPLPGWSGLVNCEVLTVEPPHRLSYRWGDGTESDSGLVTIVTWTLTPR
                     DGGTLVRMEQSGFRAPDELAFKRMGKGWPRVLEMLDDVAGRTN"
     gene            complement(1061..1384)
                     /locus_tag="VFX46_09315"
     CDS             complement(1061..1384)
                     /locus_tag="VFX46_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX5600824.1"
                     /translation="MHAEPDHLFAALADPTRRRLFERLSREGERSVRALTAGTTISQP
                     AVSKHLNVLKQAGLVRDRRVGRETRYSAHPKALRPIVDWLGVYAAFWSQKLDKLEDLL
                     DRMDQ"
     gene            complement(1465..2709)
                     /locus_tag="VFX46_09320"
     CDS             complement(1465..2709)
                     /locus_tag="VFX46_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5600825.1"
                     /translation="MTSDVLVLGAGMVGVSAALHLQKRGRSVVLVDRRGAAEETSYGN
                     AGLIQREAIVPYGFPREFSKLWRYALNMLPEAHHQWSSLPWNAVWLFKYWWHSRPERL
                     AASARAAFPLVERCIVEHRALMEEAGIADMIRETGYLKLFRTNERYDQYLREQEEVRR
                     EFGVNFEQLDPAALRKREPYLTGNFVGGLLMLDPVSVTDPSAVGKAYAKLFVQRGGKF
                     ITADARTLEPTTEGWQVHTVDGPVKAREAVIALGPWSDDVLKPLGIRVPLWVKRGYHV
                     HFRPRGDAVLNGPIFDIENGFVLAPMTRGIRLTTGAEFAHRDAPKTPVQLEKLEPIAR
                     ATFPLGERVENKPWMGSRPCLPDMLPMIGPIPGHRGLWADFGHHHLGFTLGPATGRLL
                     AEMMTGEPTFTDPWPYRVDRFR"
     gene            2814..4121
                     /gene="purB"
                     /locus_tag="VFX46_09325"
     CDS             2814..4121
                     /gene="purB"
                     /locus_tag="VFX46_09325"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003602995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX5600826.1"
                     /translation="MIPRYSRPEMARIWDPETRFRIWFEIEAHAARAMADLGIIPKEA
                     ADAIWEKGSSAKFDVARIDEIERVTKHDVIAFLTHLAEHVGPEARFVHQGMTSSDVLD
                     TCFNVQLVRAADILLADLDRVLAALKRRAFEHKNTVTIGRSHGIHAEPTTFGLKLAYA
                     YAEFSRARQRLENARAEVATCAISGAVGTFANVDPRVEEYVAKQLGLRPEPVSTQVIP
                     RDRHAMYFATLAVIASSIERLATEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVL
                     SENLTGLARMVRAYALPAMENVALWHERDISHSSVERMIGPDATVTLDFALNRLAGVI
                     ENLVVYPENMKRNLDRLGGLVHSQRVLLALTQAGLSREDAYQLVQRNAMRVWRGEGDF
                     LSLLAADPEVSAKLSREELENLFDLGYHTKHVDTIFKRVFGES"
     gene            4133..4699
                     /locus_tag="VFX46_09330"
     CDS             4133..4699
                     /locus_tag="VFX46_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600827.1"
                     /translation="MRTSDVDILLVPGWIGSDPDHWQSRWARSLRTARIIEQEDWSRP
                     NRDAWVSRIIEAVRQATRPVVFVAHSWGVAAVVHAAPKLAELGVAGAYLVAPADVDVT
                     DNILPATDSGFSPVPLEKLPFPAVLVASSDDPHCSFDRAREFAERWGAAFVPAGNAGH
                     INSESGHGPWPEGLMRFGLFLSQLNTRA"
BASE COUNT          933 a         1538 c         1542 g          943 t
ORIGIN      
        1 cttcaaccat acgtccacga acgaactgca gcgtgacttg cgccacgcgt cgtccgagat
       61 gccgagccgt gagcagatcg gattccggcg gtgcgagatc cgggccttga tctgtgttcg
      121 actgggcggc agcacctagc caaaagccca gccgattcag gtcttcctcc gatcctttcg
      181 tggaattgtt ggcgggcggt aaatcgagat tgacccagtg catgccgtgt tgagccgcga
      241 aaagcgcgat ttgcaccagc gtcgcgagtt tgtctccgga ccgggcgcct gaatttgtga
      301 agccggcagc aatcttgttc ttccaggcaa atccctttgc catgacggct ttcgaggttg
      361 cttcttggaa agctttgaac gccgcagaga cgttgcccat gtaggtcggc gagccgaaga
      421 taatcgcgtc tgctgaagcc agatcatccc atcgggtctg agcttcttcg acggtcatca
      481 acagcgcttc cgcgccttcg acctcctcga ctccgtcggt gacagcttga gccagccgag
      541 ccgtgtgacc ccagccgctg tgatacacga tggcgatgcg ggcgagcggc atgcgaatcc
      601 tccgactcga tcaattcgtg cggccggcga cgtcatcgag catttcgagc acacgcggcc
      661 agcctttgcc catgcgcttg aaagcgagct cgtctggtgc ccggaaaccg gattgctcca
      721 tgcggacaag cgttccgcca tctcgcggcg taagcgtcca ggtcacaatt gtaacgaggc
      781 cgctgtcgga ttccgtgcca tcgccccagc gatagctcag ccgatgtggc ggctcgacgg
      841 tgagcacctc gcaattgacg aggccggacc accctgggag gggcacggcg cgcatggtga
      901 agcgatggcc gactttggga acgaagtcgt tttccatcag ccattccgcg atcaaatggc
      961 tctccgttag cgcgcgccag accttttcgg gcggatgctt catctgccgc tcgaagacga
     1021 gcgagcgcgt ggcttcgcac ttgctcatgg gatcctccct ttactgatcc atgcggtcga
     1081 gcaggtcttc gagcttgtcg agcttctggc tccagaaagc agcgtagacg ccgagccagt
     1141 ccacgatcgg ccgcaaggct ttcggatggg cgctgtagcg cgtttcccgg ccgacgcgtc
     1201 gatcgcgtac gaggccagcc tgcttcaaca cgttcagatg cttagaaaca gccggctgcg
     1261 aaatggtggt gccggcagta agagcacgga cggatcgctc gccctcgcgg ctcagccgtt
     1321 cgaatagcct gcgtctggtg ggatcggcga gggcagcgaa aagatgatcc ggctcagcgt
     1381 gcatgaataa cccataactt ttcggttatg aatgactcat aaccaattgg ttatgggttc
     1441 gtcaagcggc ttagagtgcc ggtcttaccg gaagcgatcc accctgtacg gccacggatc
     1501 ggtgaacgtg ggctcgcctg tcatcatctc ggccagcaag cgtcccgtcg cggggccaag
     1561 cgtgaagccg agatggtgat ggccgaagtc ggcccacaga ccccgatggc ccgggatagg
     1621 tccgatcatc ggaagcatat ccggcaggca gggccgggag cccatccaag gcttgttctc
     1681 aacccgctcg cccaggggga aagtcgcacg ggcgatcggc tcgagcttct cgagctgcac
     1741 gggcgttttc ggcgcgtcac gatgcgcgaa ctcggcaccg gtggtcaggc gaatgccccg
     1801 agtcattggc gccagcacga agccattctc gatgtcgaag atcggcccgt tgagcacggc
     1861 atctccgcgc gggcggaagt ggacgtggta tccccgcttc acccagagcg gaacgcggat
     1921 gccaagcggc ttcaacacgt catccgacca agggccgagc gcaatcacgg cctcgcgcgc
     1981 cttgaccggc ccgtcgaccg tgtgcacttg ccagccttcc gtggttggct cgagcgtgcg
     2041 agcatcagcg gtaatgaatt tgccgccgcg ttgaacgaaa agcttggcat aagccttccc
     2101 gacagcgctg ggatcggtca cgctgaccgg gtcgagcatc agtaagccac cgacgaagtt
     2161 gccagtgagg taaggttcgc gcttgcgcag agcggcagga tcgagttgtt cgaagttgac
     2221 gccgaattcg cgccgcacct cctcctgttc tcgcaaatat tgatcgtacc gctcattggt
     2281 gcggaacagc ttcagatagc ccgtctcgcg gatcatatcc gcgatgccgg cttcctccat
     2341 gagggcgcgg tgctcgacga tgcagcgttc aacgagcggg aaggcagcgc gagctgaagc
     2401 agcgagtcgc tccggcctcg aatgccacca atacttgaac agccagacgg cgttccaggg
     2461 cagcgagctc cattgatggt gcgcctcggg cagcatgttg agcgcatacc gccagagctt
     2521 gctgaactcg cgcgggaagc cgtacggaac gatcgcttcg cgctggatca ggccggcgtt
     2581 gccgtaagag gtctcttcgg ctgcaccacg gcggtcgacg agaacgacgg agcgaccacg
     2641 tttttgcaag tgcaaggccg ccgatacgcc gaccatgccg gcgcctagga cgagaacatc
     2701 ggaggtcatg ggttagttaa ccggcagttt tcaaatgcaa tgcgcgttgg gctgcattga
     2761 tgcccagggt gtcgtctgct aggccagcgc ctgtcaatag gaggttgagc cgcatgattc
     2821 cccgctatag ccgcccagag atggcccgca tctgggatcc tgaaacgcgt ttccgcatct
     2881 ggttcgagat cgaggcccac gcggcacgcg ccatggccga cctggggatc atccccaagg
     2941 aggcggctga cgccatttgg gagaaaggct cttccgccaa attcgacgtg gcccgcatcg
     3001 acgaaatcga gcgagtcacg aagcacgatg tgatcgcgtt tctcactcac ctcgcagaac
     3061 atgtggggcc ggaggcgcgc tttgtccacc agggcatgac gtcctccgat gtgctcgaca
     3121 cgtgcttcaa cgtgcagctc gtgcgcgctg cggacatttt gctggccgac ctcgaccgcg
     3181 tgctcgcggc gctgaaaagg cgggccttcg agcacaagaa caccgtgacc atcggacgta
     3241 gccatggcat tcacgctgag ccgacgacct tcggcttgaa gctggcttac gcctacgcgg
     3301 aattctcacg cgcgcgtcag cggttggaga atgcgcgggc tgaggtagcg acatgtgcca
     3361 tttcaggcgc tgtcggcacc ttcgccaatg tcgatccgcg agttgaagaa tacgtggcga
     3421 agcaactcgg cctgaggcct gagcccgtgt cgacccaggt cattccgcgc gatcgccatg
     3481 ccatgtactt cgcgacgctg gctgtgatcg cgtcctccat cgagcggctg gccaccgaaa
     3541 tccggcacct gcaacgcacc gaggtgctgg aagccgagga gttcttctca gagggccaaa
     3601 aaggctcgtc tgccatgccg cacaagcgca atccggtact gtcagagaac ttgaccgggc
     3661 tcgcccggat ggtccgcgct tatgcgcttc ccgcaatgga aaacgtggcg ctctggcatg
     3721 agcgggacat ctcccattcg tccgtcgagc ggatgatcgg gcctgacgcg accgtgaccc
     3781 tcgactttgc cctgaaccgg ctagccgggg tgatcgagaa cctcgtcgtc tacccggaaa
     3841 acatgaagag gaacctcgac cggctcggcg ggcttgtcca ctcgcagcgt gttctcctgg
     3901 cgctcacgca agccggtctg tcgcgcgaag acgcctatca actcgtgcag cgcaacgcca
     3961 tgcgggtgtg gcgcggggag ggggatttcc tgagcttgct ggctgccgac ccggaggtca
     4021 gcgccaagct gtcgcgggag gagctcgaaa acttgttcga cctcggctac cacaccaagc
     4081 acgtcgacac cattttcaag cgggtctttg gagagagctg agcgagcccc gcatgcgaac
     4141 gtccgatgtc gacatcctac tggtgcccgg ctggatcggc tctgatccgg accactggca
     4201 gtcgcgctgg gcgcgcagcc tgcggacggc gcgcatcatc gagcaagagg attggagcag
     4261 gcccaacagg gacgcctggg tcagcaggat catagaagcg gtccggcagg cgacacggcc
     4321 agtggtcttt gttgcccata gctggggtgt cgccgcggtg gtgcatgcag ccccgaagct
     4381 ggccgagctt ggcgtagctg gagcttactt ggttgcccca gcggatgtcg acgtgaccga
     4441 caatattttg cccgcgaccg atagcggttt ctcccctgtg ccgctggaga agctcccctt
     4501 tcccgccgtt ctcgtcgcgt cgtccgacga cccgcactgc agcttcgatc gggcgcgcga
     4561 gttcgcggag cgctggggtg cagcgttcgt gcctgcgggg aatgccggcc acatcaactc
     4621 cgaatctggt cacggcccct ggccggaggg gctgatgcgc ttcgggcttt tcctttcaca
     4681 actcaacaca agggcgtgag atcaggaaca cccgccacag cggtgacgtt ctctgtcgct
     4741 tgaattttgc ccaacattca gctccacagc gaggttggca tgaagcgcag agcacacttg
     4801 ctaacttcct tcgccctcct gctcgtgaca gtcctgcccg tcctcaacac cggaggcgcg
     4861 tccgccgccc ctccaatcgc acagtccgac aggccgaagc tcgatgtgcc ctacgtgccc
     4921 acgccacggc atatcgtgga caagatgctg gagctc
//
LOCUS       DASUMG010000558         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_157266, whole genome shotgun sequence.
ACCESSION   DASUMG010000558 DASUMG010000000
VERSION     DASUMG010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157266"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFX46_09335"
     CDS             complement(<1..779)
                     /locus_tag="VFX46_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HEX5600828.1"
                     /translation="MDFKLPVGEWAEALVDFILDNFQPALDFIADVVDGFGGAIDSAL
                     TALPDWLIVTLLVGLALWRVGIPFAAFTLASLLLIMSMDLWSAMVSTLGLVMAATIFS
                     LAVGIPLGIMASRNATFESVVKTMLDFMQTMPAFVYLIPAVMFFGLGRVPGIIATVVF
                     AMPPAVRLTALGIKQVPTEIVEAARAFGCTERQLLFRVQLPTALPSIMAGVNQTIMLA
                     LSMIVIASMIGAGGLGNVVLQGIQRLDIGLGFKSGLSVVLLA"
     gene            complement(783..2015)
                     /gene="proV"
                     /locus_tag="VFX46_09340"
     CDS             complement(783..2015)
                     /gene="proV"
                     /locus_tag="VFX46_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HEX5600829.1"
                     /translation="MRKPEVAEKAPRAQAPIRLEVRGLTKVFGDDEKAALEMLAAGAT
                     KEEVHEKLGVVVGVDNASFEVRAGEIFVIMGLSGSGKSTIIRLLNRLIEPTAGAILLD
                     GRDLAQMSQRELIEVRRRDMSMVFQSFALMPHLTAIENAAFGLAVAGIPAAKRRKIAL
                     EALEQVGLAAYADRYPHEMSGGMQQRVGLARALANNPTIMLMDEAFSALDPLIRSEMQ
                     DELIRLQKTQRRTIIFISHDLDEAMRIGDRIAIMRDGRIAQVGTPYEILRNPADDYVR
                     AFFRNVNIAKVLRAGDVAHYEAANTIIRVPGELEPALQQLYENEQFYGYVKCTADRYQ
                     GIVSRDTLEREVKSRNPRFAHAFLPGIEPIRHDTLVHDILRRVAASECPVPVVDDDGV
                     YLGAISKQALLETLDREG"
     gene            2603..3277
                     /locus_tag="VFX46_09345"
     CDS             2603..3277
                     /locus_tag="VFX46_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF922 domain-containing Zn-dependent protease"
                     /protein_id="HEX5600830.1"
                     /translation="MRKNWVALVLTVAFAAAMALIGLGPGGSAAPTSSMEMSKAQETI
                     KYYDVRGSTIEELRREVYSRGPYDSTGQRFAGWAEWRIRWWFDREQVPQGCKISRAVT
                     ETDIEYTLPRWVDADNAQPELRERWNRFLEALTLHEQGHGELARALAAEIEFAIRNLP
                     PEPTCEELDRKVNELANRMILEDRTQEAYDLVTGHGQTQGAVFPDTVFVHASSEEAPI
                     QASATP"
     gene            complement(3307..>3981)
                     /locus_tag="VFX46_09350"
     CDS             complement(3307..>3981)
                     /locus_tag="VFX46_09350"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEX5600831.1"
                     /translation="GTAQGFGIRMGIAAIVMLIMLIGGRIVPSFTRNWLVRRRPGRLP
                     VPFGRLDAAIMAASGVALVSWIAMPDHTATALLMLVAGLLNVLRLGRWAGERTTAEPL
                     VLVLHVAYAFVPLGFLLLALSIYAPDILVTSGALHSWTTGAIGLMILAVMTRASLGHT
                     GQPLTATRSITAIYIATFIAAAARIVAAFDVAPEPMLHLSATAWVLGFAGFAVIYAPL
                     LTRPRQ"
BASE COUNT          854 a         1221 c         1194 g          712 t
ORIGIN      
        1 gcgagcagca cgacactaag gccgctcttg aacccaaggc cgatgtcgag ccgctgaatg
       61 ccttgcagca cgacgttacc taagccgcca gcgccgatca tcgacgcgat gacgatcatc
      121 gacagcgcaa gcatgatggt ctgattgacg ccggccatga tggacggcag cgccgtggga
      181 agctgaaccc ggaacagaag ctgccgctcc gtacagccga atgcccgcgc cgcctcgacg
      241 atctccgttg gcacctgctt gatgcccagc gcggtgagcc tcacggcagg cggcattgcg
      301 aagacaactg tcgcgatgat gccgggcacg cgtcctaggc cgaaaaacat gacggccgga
      361 atgagataaa cgaaagccgg catcgtctgc atgaaatcga gcatcgtttt cacaacgctt
      421 tcaaacgtag cgttgcgcga agccataatt ccgagcggaa taccgacggc cagactgaag
      481 atcgttgcgg ccattacgag accaagggtc gacaccatgg ccgaccacag gtccatggac
      541 atgatgagca gcagtgatgc gagcgtgaat gctgcgaacg gaatgccaac ccgccagagc
      601 gccagcccga ccaataaggt aacgattagc caatctggca gcgccgtaag cgccgagtcg
      661 atcgcgccgc cgaatccatc gacgacatcc gcgataaagt cgagtgcggg ttggaaattg
      721 tcgagaataa agtcgaccag agcttcggcc cattcgccga caggtagctt gaagtccatg
      781 gatcagccct cccgatccag cgtttcaagc aatgcctgtt tcgaaatggc tccgagatag
      841 actccatcat cgtcgacgac aggcaccggg cattccgaag cagccacgcg acgaagaata
      901 tcatgcacga gcgtatcgtg ccgaattggt tcgatgccgg gcaggaaagc gtgggcgaag
      961 cgcgggtttc ggctctttac ttctcgctcc agcgtatcgc gagaaacgat cccctgataa
     1021 cgatcggccg tgcacttcac atagccgtag aattgctcgt tttcgtaaag ctgctgcagc
     1081 gccggctcca gctctcccgg cacacggatg atcgtgtttg ccgcttcgta atgggcgaca
     1141 tctccggcgc gcagcacctt ggcgatattc acgttgcgga aaaaagcgcg aacgtaatcg
     1201 tcagcgggat ttcggaggat ttcgtaaggt gttccgacct gcgcaattcg gccatcgcgc
     1261 atgatggcga tccgatcacc gatgcgcatt gcctcgtcga gatcgtgcga aatgaaaatg
     1321 atcgtgcgcc tttgggtctt ctgcaggcgg atcagctcgt cctgcatctc cgaccgaatg
     1381 agagggtcga gggctgaaaa tgcctcgtcc atcagcatga tggttgggtt gttggcgaga
     1441 gcgcgcgcga gaccgacgcg ctgctgcatt ccgccggaca tttcgtgcgg atatcggtct
     1501 gcataggcag caaggccgac ctgctcgagc gcctccaatg cgattttccg ccgctttgcg
     1561 gctggtatcc ccgcgacggc caatccgaag gccgcatttt cgatcgccgt cagatgcggc
     1621 atcagcgcga acgattggaa aaccatgctc atgtcgcgcc gcctcacttc gatgagctcg
     1681 cgctgcgaca tctgcgcaag atcgcgcccg tccagcagaa ttgcgccggc ggtcggctcg
     1741 atcaggcgat tgagaagacg aatgatggtg gattttccgg agccggagag ccccattatg
     1801 acgaagattt cgccggccct tacctcgaag ctcgcgttat cgacgcccac cacgacgccg
     1861 agcttctcgt gaacctcttc tttggtcgcg cccgccgcga gcatttcgag cgctgctttt
     1921 tcatcgtcgc caaaaacctt ggtaaggcca cgcacctcca gccgaatcgg cgcctgggcg
     1981 cgaggtgcct tctcggcgac ctccggtttg cgcaaggccg aaccgttggc tttcacggcc
     2041 acctgcgcag taatcggcct taaacggtct atcccgtttg tcagctctgt tgcgttgccg
     2101 cgcaactgtc acctctctta ttgcgactgg cgaataccgc taaattgcgc cgacatgccg
     2161 cgacgactat tctcaccgcg acaaaaaatc cgccagcatc gacggtcgaa ctgaccgccg
     2221 ccctactcaa gaagagctac gcaaacctat ccgcccgcgg catctacggc gctgcgatct
     2281 gacatgcttg cgctgccccc ctccaacgat gccgacgcgc aaagagcacg tcacaggggc
     2341 ggacccgatc cgccactgaa ggcagatcat caagggccct gtgaaacgga ctacgggggc
     2401 gcgatggccc catgcccgcc ccgcgtatgc cacatcgagc ccgccttgtc agccaaaaat
     2461 ggcccaggtc ccgacaaagt tacagtatgg ttgccgatcg tgagcggctg gcgacggctg
     2521 gtttacctgt cagctcaacg gcttgtccga tttttgccgc atcttgcgat gaaagttgct
     2581 gtagcaagcc gtggggggat gcatgagaaa aaattgggtg gccctcgttc tgacggttgc
     2641 cttcgcagcg gctatggccc tcatagggct cgggccggga ggcagcgcag cgccgacctc
     2701 cagcatggag atgagcaagg cgcaggagac gattaagtac tacgatgttc gcggaagcac
     2761 gatcgaagag ctgcggcgcg aggtctactc acgcggcccc tacgacagca ccgggcagcg
     2821 ctttgccggt tgggcggagt ggcggatccg gtggtggttc gatcgcgagc aggtgccgca
     2881 aggctgcaag atttcacgag ccgtaaccga gaccgacatc gaatacacgc ttccccgctg
     2941 ggtcgatgca gataatgccc agccggagct ccgtgagagg tggaaccgtt tcctcgaagc
     3001 gctcacgttg cacgagcaag ggcacgggga gctggcccgc gctcttgctg cagagatcga
     3061 gttcgccatt cgaaatctgc cgcctgagcc gacctgcgag gagctcgatc ggaaagtcaa
     3121 cgagctcgcc aaccgcatga ttttggagga caggacccag gaagcctacg atctggtcac
     3181 cgggcatggc caaacgcaag gtgccgtctt ccctgacaca gtcttcgtcc atgcctcatc
     3241 cgaagaggcg ccgatacagg cgtcggcgac cccgtaagga tcggagtttg cagaccgatg
     3301 tcggggctat tggcgcggcc gcgtcagtaa cggcgcgtag atcacggcga atcccgcaaa
     3361 gcccaatacc caggccgtgg cggaaaggtg cagcatcggt tcgggggcaa catcgaacgc
     3421 ggcaacaatg cgtgccgccg cggcaatgaa cgtcgcaatg taaattgcgg taatgctgcg
     3481 cgtagctgta agcggttgcc ccgtgtgccc gaggctggcg cgcgtcatca cggccaggat
     3541 catcaggccg atcgcccccg tggtccagct atgcagcgcg ccagacgtaa ccagaatatc
     3601 cggcgcatag atgctgaggg ctaacagcag gaacccaaga ggcacgaacg cataggcgac
     3661 gtgaagaacc agcacgagcg gctcagccgt agtacgttcc cccgcccatc ggccgagacg
     3721 taagacgttg agaagccccg cgacgagcat cagtagagcc gttgcggtgt ggtctggcat
     3781 tgcgatccaa ctcacgagag ccacgccgct ggccgccatg attgcggcgt ccagacgccc
     3841 gaagggcaca ggcagccgtc caggccgccg ccgaacaagc cagttgcgcg tgaagctggg
     3901 cacgatccgc ccgccgatga gcatgatgag cattacaatg gccgcgatgc ccattcggat
     3961 cccgaagccc tgcgccgtgc c
//
LOCUS       DASUMG010000559         5013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1041113, whole genome shotgun sequence.
ACCESSION   DASUMG010000559 DASUMG010000000
VERSION     DASUMG010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041113"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFX46_09355"
     CDS             <1..649
                     /locus_tag="VFX46_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX5600832.1"
                     /translation="AGRLEARYHHEPARDSPVALILHPHPQFGGTMNNQVVYHLYYTF
                     VERGFSVLRFNFRGVGRSQGFFDNGPGELADAASALDWLQMAKPEAKSCWIAGVSFGT
                     WIAMQLLMRRPEIDAFVCVAPPANLYDFSFLAPCPASGLLVNGEKDRVVPSASVAELA
                     AKTKTQKGIKIEHEIIPGANHFFEGEDKIAALKQVVGAYIDRRLPEILRAKAEAK"
     gene            complement(702..1853)
                     /locus_tag="VFX46_09360"
     CDS             complement(702..1853)
                     /locus_tag="VFX46_09360"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018208328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX5600833.1"
                     /translation="MGKLLRALGLMSGTSMDGIDVALIETDGEHEVVRGPHATYGYPP
                     EFRERLREATNDAHALTDRRARPGRLGEVERELTERHAEAVAAFLTDTAISSTEIDVI
                     GFHGQTVLHRPASRATSFGNGADSQSPPITVQLGDGELLAKLTGIDVVYDLRAADVEA
                     GGQGAPLVPVYHSALAAKLPQRPLAFLNIGGVANVTWIGGDGRLIAFDTGPGNALLDD
                     WVLRHTGRPYDANGNLARSGKVHEDVLQQYLMHPHFEQPVPKSLDRNDFTLDLVEGLS
                     PADGAATLAQLTAAAVARSLSWLPEPPVLWLVTGGGRRNKHIMELLAWHLEAPVAPVE
                     AVQLDGDSMEAEAWAYLAVRSLKGLPITYPGTTGVPKPLTGGIVAAAPA"
     gene            1928..3184
                     /gene="tyrS"
                     /locus_tag="VFX46_09365"
     CDS             1928..3184
                     /gene="tyrS"
                     /locus_tag="VFX46_09365"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX5600834.1"
                     /translation="MPTPRSDFIRTLAERGFIHQCSDLEGVDALAAKRELVAYVGYDC
                     TAPSLHVGHLLSIMMLYWLQQTGAGKPIALMGGGTTRVGDPSGRDETRRLLSLEEIDA
                     NKESIKKVFSRFLRFGPGRDDAIMVDNAEWLVPLNYIDFLRDVGRHFSVNRMLTMDSV
                     RLRLEREQELSFIEFNYMILQAYDFVELARRYGCNLQMGGSDQWGNIVTGIDLGRRMG
                     THQLYALTTPLLTTASGAKMGKTAAGAVWLNEDQLSPYEYWQFWRNTEDGDVARFLKL
                     FTTLPMDEINRLAALQGAEINEAKKVLATEATALVHGREKAEAAAETARATFEEGALA
                     VGLPTVEIPRAEIADGLGVLAAFVRAGLVKSNSEARRQIQGGGLRVNDTVVTDERARL
                     TPADLTADNVIKLSLGKKKHVLLRAV"
     gene            complement(3209..>5013)
                     /locus_tag="VFX46_09370"
     CDS             complement(3209..>5013)
                     /locus_tag="VFX46_09370"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX5600835.1"
                     /translation="APAVIELIEHEAFGIGRLAAIENAGIDGKFEADLTITVPLHRSI
                     TLASLKVEGQGKLTEGSARKLIGKHDAQAATIAFDINEKAITASGDLLIAGVPVKLAW
                     QHIFGALPERQPPLRLSATLDRVDRDQLGLDPRHMLDGEVPVEVTITQSAEEEHLIHV
                     WADLTRADVVLDSVIWRKRPGRAAIMEFDLRPGTRYPLELRDFKIVGDDIAISGWVGL
                     DEEERLNEFHFPEFSASIITRLDVKGVLGENNIWRIKAEGPTYDGREVFRSLFSVGDL
                     DEPDRGRKKPAGVDLEANISTVIGFSDVALRNFKLRLSEREGKLTALTGNGVLDGGQP
                     LTLELQHGPDRSRRLVALTDDAGQAFRLVDFYPSLVGGRMRLDVNLDGQGAAEKTGLL
                     RVERFRILGDPIVTEVLQVQNDGRSTGSQRERRVIRQAFDFDWMRMPFSIGHGQFVIG
                     ESEIRGPLVGATLRGKADFRSRQVNIGGTYVPLQGLNAALGAIPGLGQIIAGPRGEGI
                     LGITFAIRGPMANPEVLVNPLSLVAPGIFREMFQMTPSQSVTPPRAAPQPSTGPRSSR
                     TPAGSKAQRAENVSPEVLSGWSSETRSNPPTKTK"
BASE COUNT          918 a         1592 c         1587 g          916 t
ORIGIN      
        1 tgctggccgt ctagaagccc gctatcatca cgagccggca cgcgacagcc cggtcgccct
       61 gatcctccat ccgcatccgc agttcggcgg gacgatgaat aatcaggtgg tgtatcacct
      121 gtattacacg ttcgtcgagc gcggtttctc agtgctccgc ttcaacttcc gcggtgtcgg
      181 gcgcagccag ggcttcttcg acaatggtcc tggtgagctt gccgatgccg cgtcggcgct
      241 ggactggctg caaatggcca agccggaggc gaagagctgc tggatcgccg gcgtctcgtt
      301 cggcacgtgg attgccatgc agctcctgat gcggcggccg gagatcgacg cgttcgtctg
      361 tgtggcgccg ccggccaacc tgtatgactt ctcgttcctg gcgccctgcc cggcatccgg
      421 cctgctggtc aacggcgaga aggatcgggt cgtgccctct gcgtccgtcg ctgagctcgc
      481 ggcgaaaaca aagacgcaga agggcatcaa gatcgagcac gagatcatcc cgggcgccaa
      541 ccacttcttc gaaggcgagg acaagattgc cgctttgaaa caggttgtcg gcgcctacat
      601 cgaccgccgc ctgccggaga tcctgcgggc caaggccgaa gcaaagtaag acaaaattgc
      661 gttcgttggg tgccgcctgc gtctgaggac gcgggcggct atcacgccgg agccgcggca
      721 acgatgccgc cagtcaaagg cttcggaacg cctgtcgtgc ccggataggt gatgggcagt
      781 cctttcagcg agcggacggc gagataagcc cacgcttcgg cctccatgct gtcgccgtca
      841 agttgcacgg cttcgaccgg cgcaaccggt gcctcgaggt gccaggctag cagctccata
      901 atatgcttgt tccggcgccc cccgcctgtg acgagccaga gcacaggcgg ctccggcagc
      961 cagctcaagc tgcgggcgac ggcagcagcg gtcagctggg cgagagtcgc tgctccatcg
     1021 gctggggaga gtccctccac taggtcgagg gtgaaatcat tccggtcgag cgatttcggc
     1081 acgggctgct cgaagtgagg atgcatcagg tattgttgca gcacgtcctc atgcactttg
     1141 ccggatcggg ccaaattgcc gttggcgtca tacggccggc ccgtgtgccg cagcacccaa
     1201 tcatcgagga gcgcgttgcc cggaccggta tcgaaggcaa tcagccggcc gtcccctccg
     1261 atccacgtga cgttggcgac gcctccgatg ttcaaaaacg ccagtggccg ctgcggcagc
     1321 ttggccgcca aggcgctgtg gtagacgggc accaatgggg cgccctgccc tccggcctcg
     1381 acatccgcgg cgcgcaaatc atagacaacg tcgatgcccg tgagcttcgc caggagctcc
     1441 ccgtctccaa gctgtacagt gatagggggc gattgcgagt ccgcgccatt accaaacgat
     1501 gtcgcgcgcg atgccggccg atggagcacc gtttggccgt gaaacccgat cacatcgatc
     1561 tcggtggagc tgatggccgt gtcggttaga aaggctgcaa cagcctctgc gtggcgctcc
     1621 gtcaattcac gctcgacctc acccaagcgg cctggccgag cccgtcgatc cgtcagcgcg
     1681 tgggcatcat ttgtggcttc gcgcaagcgc tccctgaact caggcgggta cccgtacgtg
     1741 gcatggggtc cccgtacgac ctcatgttcg ccgtccgtct cgatgagcgc cacgtcgatg
     1801 ccgtccatcg acgtgccgct catgagaccc aatgcccgta ggagcttgcc catctgcacc
     1861 ttcccgtgca tgttcgcgcg tcatcgtaga acgaaattcg agacgccgat acctggcgca
     1921 cccgcacatg ccgacaccga gatccgattt cattagaacg ctcgccgagc gtggcttcat
     1981 ccaccagtgc tccgatcttg agggcgtcga cgccctcgcg gccaagcgcg agctggttgc
     2041 ctacgtgggc tacgactgca ccgctccgtc gctccacgtc gggcatctgc tgtcgatcat
     2101 gatgctgtac tggcttcagc agacgggagc cggcaagccg atcgctctca tgggtggggg
     2161 tacgacgcgc gtgggcgatc cctcgggtcg cgacgaaaca cgccggctgc tttctctcga
     2221 agagatcgat gccaacaagg agagcatcaa gaaggtcttc agccgctttc tgaggttcgg
     2281 cccggggcgt gacgatgcga tcatggtcga caacgccgag tggctcgtcc cgctcaatta
     2341 catcgacttc ctgcgagatg tcgggcgcca cttctcggtc aaccgcatgc tgaccatgga
     2401 ttcggtgcgg ctgcggctcg agcgcgagca ggagctgtcg ttcatcgagt tcaattacat
     2461 gatcctgcag gcctacgact tcgtcgagtt ggcgcggcgg tatggctgca acctgcagat
     2521 gggcggctcg gaccagtggg gtaacatcgt caccggtata gatcttggcc ggcgcatggg
     2581 cacgcatcag ctctacgcct taacaacccc actccttacg accgcctccg gcgcgaagat
     2641 gggcaagact gccgcgggtg cggtttggct caacgaggat cagctcagcc catacgagta
     2701 ctggcaattc tggcgcaata cggaggatgg ggacgtcgcc cgattcctga aactgttcac
     2761 cacgttgccg atggacgaga tcaacaggct cgcagccctt cagggcgcgg agatcaacga
     2821 ggcgaagaag gtactggcaa cggaagcaac agcgctcgtc catggccggg agaaagcgga
     2881 agcggccgct gagacggcgc gggcgacgtt tgaggaagga gccctcgccg tcggcttgcc
     2941 gacggtcgag attccgcgag ccgagatcgc cgacggcctt ggcgtgctgg cggcgttcgt
     3001 tcgcgccggc ctggtcaaga gcaacagtga ggcccggcgc cagatccagg gcggcggcct
     3061 gcgggtcaac gacacggtcg tgacggatga aagggcgcgc ctcacaccag cggacctgac
     3121 ggctgacaac gtcatcaagc tctccttggg caagaagaag cacgtgctgt tgcgcgcggt
     3181 ctgaatgtcc caggacggcg cccgcagatc acttcgtttt ggttggcgga ttcgaacgcg
     3241 tctctgagga ccagcccgat agcacttcag gtgagacatt ctccgcgcgc tgggccttcg
     3301 agcccgccgg agtccgggat gagcgcggtc cggttgatgg ctgcggcgcg gctcggggcg
     3361 gtgtcaccga ctggctcggt gtcatctgga acatctcgcg gaaaatgccg ggggcgacga
     3421 gggagagtgg attgaccagc acctcggggt tcgccatcgg ccccctgatc gcaaaggtga
     3481 tcccaaggat gccctcaccc cgaggcccgg cgatgatctg cccgagccct ggaatagcgc
     3541 cgagcgccgc gttcaagccc tgcagaggaa cgtaggtccc gccgatgttg acttgccgcg
     3601 agcggaaatc ggccttgccc cgcaacgttg cgccaaccag cggaccacgg atttccgatt
     3661 caccgatcac gaactgcccg tgcccgattg agaatggcat gcgcatccaa tcgaagtcga
     3721 acgcctgccg gatcacgcgg cgctcgcgtt ggctaccggt agatcggcca tcattctgaa
     3781 cctgcaacac ctcggttacg atggggtcgc cgaggatccg aaaccgctcg actctcaaga
     3841 gacccgtctt ctcggcggca ccttgcccat cgagattgac gtcgaggcgc atgcgcccgc
     3901 cgaccaggct cgggtaaaaa tcaaccagcc ggaacgcctg gcccgcgtcg tccgtaagcg
     3961 cgacaagtct ccgcgacctg tccggcccat gctgcagctc gagggtgagg ggctggccgc
     4021 cgtcgagcac accgttccct gtgagtgccg tcagcttgcc ttcacgctcg gagagccgca
     4081 gcttgaagtt gcgcagggct acgtcggaga agccgatgac ggtcgagatg ttggcttcga
     4141 gatctacgcc ggccggcttc ttgcggcccc gatcgggctc gtcgagatcg cccactgaga
     4201 acagcgaacg gaagacttca cggccatcat aggtcggccc ttccgctttg atccgccaga
     4261 tattgttctc acccagcacg cctttcacat cgaggcgggt gatgatgctg gcggagaact
     4321 cggggaagtg aaactcattg agacgctcct cctcgtccag ccccacccat ccgctgatcg
     4381 caatgtcgtc gccaacgatc ttgaagtcgc gcagctccag cggatagcgg gtccccggtc
     4441 gaagatcgaa ctccataatg gcagctcgtc ccggtcgctt tcgccaaatg acgctgtcca
     4501 acaccacatc ggcgcgtgtg aggtccgccc agacatggat gaggtgctcc tcctccgcgc
     4561 tttgagtgat cgtgacctcc acgggcactt ccccgtccag catatggcgg ggatctagac
     4621 cgagctgatc gcgatcgacc cgatccaagg tggccgaaag gcggagcggc ggctgtcttt
     4681 ccggtagcgc gccgaaaatg tgctgccagg cgagcttcac gggcacgccg gcgatgagaa
     4741 gatcgccgct ggcggtgatg gccttttcgt tgatatcgaa ggcgatggta gccgcctgag
     4801 cgtcatgctt gccgatcagc ttacgggcgc tgccctcggt cagtttgccc tggccttcca
     4861 ccttcaaact cgcaagcgtg atgctgcggt gaagggggac cgtaatcgtc agatcagcct
     4921 cgaacttgcc atcgatgccc gcgttctcga tcgcagcaag gcgcccgatg ccgaaggcct
     4981 catgctcgat gagctcgatg acggcaggtg cgg
//
LOCUS       DASUMG010000560         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_879202, whole genome shotgun sequence.
ACCESSION   DASUMG010000560 DASUMG010000000
VERSION     DASUMG010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879202"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1129
                     /locus_tag="VFX46_09375"
     CDS             281..1129
                     /locus_tag="VFX46_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX5600836.1"
                     /translation="MDPFVFFAVLAAAACHAGWNALLKLKLDPVTTVSLINACCGIVV
                     LPFVFVAQLPELAAWPFLIASVLLHLAYFLSLAEAYRFGDLSHVYPIARGTAPLLTAV
                     GSTFLVGEPLGTLGWLGVILLAGGIMALSIRPRDRSLDAFHGRSVAFALLTSITITSY
                     TLVDGLGARIGPSPAPYIVWLFLLNGIVMAAYGLIRVPAAMVQGAKSSWYMALTGGVL
                     LTSAYAIAIWAMTVAPIALVAALRETSVLFATLIGIVFLREPVYALRIVAGCVVLVGI
                     VLLRLR"
     gene            complement(1137..>2081)
                     /locus_tag="VFX46_09380"
     CDS             complement(1137..>2081)
                     /locus_tag="VFX46_09380"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEX5600837.1"
                     /translation="LDWAVVRMQHLSADPGRLVLNFHTAFNLVVALIFLPLLSPLGKL
                     VTWLLPTPAGAPDRSVPRHLDSSVLDTPSEALGCAMRETLRLGDIVLDMLRKSLPAIE
                     GNDLRLVKEVEKADDDVDALHEAIKLYLIRTSKNELDDDESRRYYEILTFTTNLEHVG
                     DIIDKNLMELATKKIKKGYSFSPEGLSELKRLHARVVDNMRLALNVFATRDVDLARRL
                     LMEKINLRSKEFEAAERHFARLKEGRPESIETSAIHLDVIRDLKRINSHLTAVAYPIL
                     EAAGQLRESRLLEKQREEARERAEAPDASLAPSPQRPA"
BASE COUNT          304 a          703 c          652 g          422 t
ORIGIN      
        1 ctacgaccaa ccggttaaca gccggtcgct ctaccactga gctaccgcgg atcacgtcat
       61 cgttcgagat ggccggctca tagcagaacc tcgccgcagg ggctagtccc ctccgccgtc
      121 atgtcgcgac gggctgcgcc gtgagccacc ttcggcgctg cgagcccgcc cgagccgccc
      181 tccagcacgc aaagggatga caactccctg gcaattcggc tacgagtgcg ccgcccccca
      241 ctcgcgccgc acctcaagag tcctcggccg gagctggagt atggacccct tcgtcttctt
      301 tgcggttctg gccgcggctg cctgccacgc cggatggaac gcgctgctca agctgaagct
      361 cgatccggtg acgacggtca gcctgatcaa cgcctgctgc ggcatcgtcg tgctgccgtt
      421 tgtcttcgtc gcccagctgc cggagctcgc cgcctggccc ttcctgatcg cgtcggtgct
      481 tctgcacctt gcgtattttc tctctctggc cgaggcctac cgcttcggcg acctctcgca
      541 tgtctacccg attgcccgcg ggacggcgcc gctgctgacc gctgtcggct cgacgttctt
      601 ggtcggagag ccgctgggca cgctcgggtg gcttggcgtc atccttcttg cgggcggcat
      661 catggcgctc tcgattcgcc cgcgcgaccg gagccttgac gcgttccacg gccggtcggt
      721 ggcgttcgcc ctactcactt cgattacgat tacgagctac acccttgtag atgggctggg
      781 cgcccgcatc ggtccatccc ctgccccgta catcgtctgg ctgttcctgc tcaacgggat
      841 cgtgatggcc gcctacggcc tcatccgcgt gccggcagcg atggtccagg gtgcgaaatc
      901 ctcgtggtac atggccctga caggcggagt gttgttgacg tctgcctatg cgatcgcgat
      961 ctgggcgatg acggtggcgc ccatcgcgct cgtggctgcg cttcgcgaaa cgagcgtgct
     1021 cttcgcgacg ctcatcggca tcgttttctt gcgcgagccg gtctacgccc tgcggatcgt
     1081 cgctggctgt gtcgtgctcg tgggcatcgt tctgctgcgg ctgcgctgac gccctctcat
     1141 gcaggccgct ggggactcgg cgcgagagac gcgtcgggag cctcggcccg ctcgcgggct
     1201 tcttcccgct gcttttcgag aagccggctc tcccgcagct ggcccgcggc ctccaggatc
     1261 ggataggcga cagccgtcaa atgcgagttg atccgcttga ggtcgcgaat gacgtcgagg
     1321 tgaatggcgc tcgtctcgat ggactccggg cgcccttcct tcagccgcgc aaagtgccgc
     1381 tcggcggcct cgaactcctt gctccggagg ttgatttttt ccatcagcaa ccggcgcgcc
     1441 aggtccacgt cccgtgtcgc gaagacgttg agcgccagcc gcatgttgtc gaccacacgg
     1501 gcatggagcc gcttcagctc agaaaggccc tcaggagaga aggagtagcc cttcttgatc
     1561 ttcttggtcg cgagctccat caggttcttg tcgatgatgt cgccgacgtg ctcgaggttc
     1621 gtcgtgaacg tcaggatctc gtagtagcgg cgtgactcgt cgtcatccag ctcgttcttc
     1681 gacgtccgga tcaggtacag cttgatcgcc tcgtggaggg cgtcgacatc gtcgtcggcc
     1741 ttctcgacct ccttgaccag cctcaggtcg ttgccctcga tggcaggcag cgacttgcgc
     1801 agcatgtcga ggacgatatc cccgaggcgc agggtctcgc gcatcgcaca ccccagcgcc
     1861 tccgaaggcg tatcgagcac gctgctgtcg agatgccggg gcacagacct gtccggcgca
     1921 ccggcgggcg tgggaaggag ccacgtcacc agctttccga gcggggatag cagtggcagg
     1981 aagatcagcg cgaccacaag gttgaacgcc gtgtgaaagt tcagcaccag tcggccggga
     2041 tcggcgctga ggtgctgcat ccgaaccacc gcccaatcca g
//
LOCUS       DASUMG010000561         2801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_718750, whole genome shotgun sequence.
ACCESSION   DASUMG010000561 DASUMG010000000
VERSION     DASUMG010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718750"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFX46_09385"
     CDS             <1..363
                     /locus_tag="VFX46_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600838.1"
                     /translation="SRLVECSQMTARRTGKMTVEEAGRKGGEATSTLHGRSFYEEIGQ
                     KGGAARRGQLGREGYSALGRKGGEATNVRHGSGFYAEIGRKGGEAVSRNREHMSQIGR
                     KGGEARAEKGEQRLRERQ"
     gene            378..584
                     /locus_tag="VFX46_09390"
     CDS             378..584
                     /locus_tag="VFX46_09390"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX5600839.1"
                     /translation="MARGVRNISPADLEHYLAGVSYPASKDDLISAARRNEAPDEIVE
                     AIWRLPKNKFRGPQEVQKAYGDIV"
     gene            647..1009
                     /locus_tag="VFX46_09395"
     CDS             647..1009
                     /locus_tag="VFX46_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEX5600840.1"
                     /translation="MAKNRSDGGGKMTVEEAGRRGGAATAQARGREFYREIGRKGGEA
                     RRGKLGSEGYRELGRKGGAATSQKRAPVRESEHLAGISQTQQAIANEETSPGLSETGD
                     IALEEERPKDPAEPVRGT"
     gene            1302..2114
                     /locus_tag="VFX46_09400"
     CDS             1302..2114
                     /locus_tag="VFX46_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX5600841.1"
                     /translation="MVAGGLGHLLSFDITPTRMWEEIIFYVLVGFFAQTIDGALGMAY
                     GVTVSSVLLSTGVPPAVTSASVHAAEVFTTATSGFAHWCAGNVDRRLILRLVIPGMIG
                     GAIGAYVLSNVPGEMIRPWVSGYLLVLGFIILSKAFRPRPHHVDHPKGVGALGFFGGI
                     LDAMGGGGWGPLVTSTLLGYGAAPRTVIGSVNLTEFFVTTTISITFIATIGLSLWPII
                     VGLVIGGAIAAPLAALAVRYFPPRVLMAIVGCVVIGLSSWTIWGVITSQFSN"
     gene            complement(2232..>2801)
                     /locus_tag="VFX46_09405"
     CDS             complement(2232..>2801)
                     /locus_tag="VFX46_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596601.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEX5600842.1"
                     /translation="LVPDEYLGGVLKLCQDRRGRQKNLTYAGNRAMLVYELPLNEVVF
                     DFYDRLKSVSRGYASFDYQMIGYEEGDLVKMSILVNGEPVDALSIIVHRSRAEQRGRA
                     MCEKLKELIPPHLFQIPIQAAIGGRIIARETIRALRKDVLAKCYGGDVTRKRKLLEKQ
                     KAGKKKMREFGKVEIPQEAFIAALKMDEN"
BASE COUNT          570 a          797 c          891 g          543 t
ORIGIN      
        1 tcgcgattgg tggagtgctc tcaaatgact gccagaagaa ccggcaaaat gacggtcgag
       61 gaggccggac gcaaaggggg ggaagcgaca tccacacttc acggtcgcag cttctacgag
      121 gaaatcggcc agaagggcgg agcggcacgg cgtggccagc tcggccgtga aggctattca
      181 gcgctcggcc gcaagggagg cgaggcaacc aacgtcaggc atggctctgg cttctatgcc
      241 gaaatcggca ggaagggcgg cgaagccgtt agccggaacc gcgaacatat gagccaaatc
      301 gggcgcaagg gaggtgaggc gcgggcggag aagggcgaac agcgtttgcg cgagcgccaa
      361 tagtcaggga tgagcaaatg gccaggggcg tccgcaatat ctcgcctgcc gacttggagc
      421 actacttggc aggcgtttcg tacccggcca gcaaggacga cctgatctca gctgcgcgcc
      481 gaaacgaagc accggatgag atcgtggaag cgatttggcg gctccccaaa aacaagttca
      541 gaggcccaca ggaggtgcag aaagcctatg gcgacatcgt gtgatgaggc cggcactgcg
      601 gatcggcgtc attccctgtg tattgcgtaa caagaggctg tgtgtcatgg caaaaaacag
      661 gtccgacggc ggcggaaaga tgaccgtaga ggaggctggc cggagaggcg gtgcagccac
      721 agcacaggcg cgtggaagag agttctatag ggagatcggc cgcaagggcg gagaggctcg
      781 ccgcggcaag ttagggtcag agggttatcg cgagctcggc cgcaaaggcg gcgcggcaac
      841 atctcagaag cgagcccccg ttcgagaatc ggaacacctt gctggaatca gtcagaccca
      901 gcaggcgatc gccaacgagg aaacctcgcc tggcttgtcc gagaccggtg atatcgctct
      961 cgaagaggag cggccgaagg atccggcaga gcccgtgagg gggacgtaag caggctcggt
     1021 ctatcaattt ccgcgaatgg actgagcagc gctcgaacgc gtgtacccca gagggcgctg
     1081 ctcctggtag ccctcgctgc gtcaccaacg cctcggcaaa ctccagcggc agctggccgg
     1141 gaccaatatc tgcgcgctgg ccccttccat tcccgtggtg tggtttcgcg atcgccaagt
     1201 acgcgcgtcg tcgggaatgc ataggcgctc gatgcctcag gcagatcagc tcgaagggtt
     1261 gagccggtca aaccctcatc tcatctgcaa atgactgaag cttggtcgca ggtggactgg
     1321 gacacctgct ctcgttcgac ataacgccga cgcgcatgtg ggaagaaatt atcttctatg
     1381 ttctggtggg gtttttcgcc caaacgatcg atggcgcgct gggcatggcc tacggcgtga
     1441 cagtgtcgtc agtgctgctc agcaccggcg tgccaccggc ggtcaccagc gcctccgtac
     1501 acgcggccga ggtgttcacg accgcaacat cgggttttgc acactggtgt gctggcaacg
     1561 tggaccgtcg cctcatcctt cggctggtga ttcccgggat gatcggcggc gcaatcggcg
     1621 catacgtgct gtcgaacgta cctggcgaaa tgatccggcc ctgggtcagt ggctatttgc
     1681 tcgtgctggg cttcatcatc ctgagcaaag cttttcgacc tcgcccgcac cacgttgacc
     1741 acccaaaggg agtcggagct cttggttttt tcggcggcat tctcgacgcg atgggcggcg
     1801 gtggctgggg tccgctcgtc acctctacgc tcctcggcta tggcgctgcg ccgcgcacgg
     1861 tcatcggctc ggtgaatctg accgagttct tcgtgaccac aacgatctca atcactttca
     1921 tagccacgat cggcctttcg ctttggccca tcatagtggg gctcgtgatc ggaggcgcaa
     1981 tagctgcgcc actcgcggcg ctagcagttc gctattttcc gccgagagtt ctgatggcga
     2041 ttgtggggtg tgttgtgatc ggcctcagca gctggacaat atggggagtg atcacgagcc
     2101 aattctcgaa ttgattactt gagttcgaga gcttgcgcgg ccagatcatt aaagcctggt
     2161 tccgaaaagc cgatttgctg ggataagcgc ctcttccaag acattcgatc gtagggcaga
     2221 gatatccgcc tttaattctc atccattttc agcgccgcaa tgaaagcctc ttgcgggatt
     2281 tccacctttc cgaattcgcg cattttcttc ttgccggcct tctgcttctc gagcagcttg
     2341 cgcttgcggg tgacgtcacc gccgtagcac ttggccagca cgtccttccg aagggcacgg
     2401 atggtttcgc gcgcaataat ccggccgccg atcgccgcct gaatggggat ctggaagaga
     2461 tgcggcggga tgagctcttt gagcttttcg cacatggccc ggccgcgctg ctcggcgcgt
     2521 gaacgatgga cgatgatgga aagcgcatcc accggctcac cgttgaccag gatcgacatc
     2581 ttgacgagat cgccctcctc gtagccgatc atctgatagt cgaaggaggc gtagccgcgg
     2641 ctcacagact tgagccgatc gtaaaagtcg aacaccacct cattgagcgg cagctcgtaa
     2701 accagcatgg cgcggttgcc agcgtaggta aggttcttct gccggccccg acggtcctgg
     2761 cagagtttga gcacgccacc gagatattcg tccggcacga g
//
LOCUS       DASUMG010000562         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_77669, whole genome shotgun sequence.
ACCESSION   DASUMG010000562 DASUMG010000000
VERSION     DASUMG010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77669"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VFX46_09410"
     CDS             complement(<1..720)
                     /locus_tag="VFX46_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600843.1"
                     /translation="MNRMGDFTDSALARAEVPRVRTPKFDRPIQGKSTISEVFSDHLD
                     ALEAKAAVLANEAEPPSDAEKAFGAEKEGAADEVESASRVNDAAEMGLVARRFSHLFE
                     IGVKNDRAAEQSLEADADESVLTRPTANGSETRPAVPLRIDRAPISFGASPLKAADNN
                     SATERRPAWTNGRLSTPTDVALANGITLETAQTGLDRTSTLSQTQLATNATPRHQPAQ
                     PVSHQPTASDGAKPVLNSADMQ"
     gene            complement(717..1907)
                     /locus_tag="VFX46_09415"
     CDS             complement(717..1907)
                     /locus_tag="VFX46_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein MotC"
                     /protein_id="HEX5600844.1"
                     /translation="MSRLKGIVFILVLLLAAAMAPHAAGSTKAPFELIRELRNLQDAL
                     AQGDLIDHKAQQELMAKLRAQLAAVDPEVWREPRNLRAVALYGFSGGDPSILRRISSL
                     NGLPKPEAELARAALAYSEGRNEEAREILLGMDARKSDPITAGTLALVQAALIDKSAP
                     DKALVYLDQARLLSPGTLIEESALRRQVLLLISVGRLDEIDRLLRRYVRQFGKSYYAG
                     AFWRQLSAEIASGEYWDDPGRMATLEVLLATFDRNDRRHVCELLAQEGIARGNIELVR
                     FAASLLLQMAKPGDAAAPRAQLYEAAVLIVTDEYERGYAALTQLGREALDREDAELLD
                     AAISLARDIRREPAVAPNPRNLEQSAADDDRLRALSSIGADTMERARTLAASVDALVS
                     GGKR"
     gene            complement(1904..2314)
                     /locus_tag="VFX46_09420"
     CDS             complement(1904..2314)
                     /locus_tag="VFX46_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX5600845.1"
                     /translation="MAKIPHVEVVRTADGILISLTDEFDFGMFAIASAEPRPELIVIM
                     EKIARVLKDYPGRLIVRGHTDGRPFRTQTYDNWRLSTARAHMAYLLLTRGGVDERRFE
                     RIEGHADRSLRVAHDPEAAQNRRIEILLRSEEQS"
BASE COUNT          489 a          773 c          725 g          592 t
ORIGIN      
        1 ctgcatatca gctgagttaa ggaccggctt agcgccatcg ctggccgttg gctgatggga
       61 cactggctga gcgggctggt gtcgcggcgt agcattcgtg gccaactgcg tctggctcag
      121 cgttgatgta cgatcaagcc cagtttgagc tgtttcgagc gtgatgccgt tcgcaagggc
      181 aacatctgtt ggcgtactta atctgccatt cgtccacgcg gggcgtcgct ccgtcgcgct
      241 gttgttatct gcagctttca atggtgacgc cccaaaagaa attggggccc ggtcaatgcg
      301 taagggaacc gcaggcctgg tctccgatcc gttcgctgtc ggtcgggtga ggacactttc
      361 gtctgcatct gcctctaatg actgctcagc ggccctgtca tttttcacgc caatttcgaa
      421 caagtgcgaa aacctccgag ctaccagccc catctcggct gcatcattca cgcgtgaagc
      481 agactcaact tcgtcagccg cgccttcctt ttctgcgccg aaagcctttt ccgcatcact
      541 ggggggctca gcttcgttcg cgagcacagc cgctttcgct tccaaggcat caaggtggtc
      601 agagaaaacc tcggaaatcg tactcttccc ctgaattggg cggtcaaatt tcggtgtacg
      661 taccctcggc acctcagccc gggccaaggc gctgtcggta aagtctccca ttcggttcat
      721 cgctttcccc cgcttaccag cgcatcgacg ctcgccgcca gagttcgcgc gcgctccatg
      781 gtgtcggctc cgatgctgga cagcgccctt aatcgatcat cgtcggcggc gctttgctcc
      841 aagtttctag gattaggggc aaccgcaggc tcgcggcgga tgtcacgagc taaggaaatc
      901 gcggcgtcga gaagctcggc atcttctcta tcgagcgctt cacgcccgag ctgggtcaat
      961 gcggcgtagc cgcgctcgta ctcgtcggtc acaatgagca ccgctgcttc gtaaagctgc
     1021 gcccgcggtg cggcagcgtc gcctggtttc gccatttgta gcaagagtga agcagcgaat
     1081 cgcacaagct caatgttgcc ccgagcgatc ccctcctgag ctaagagttc acaaacgtgc
     1141 cgcctgtcgt tcctatcgaa tgttgcaaga agtacctcta gcgtcgccat tcggcctgga
     1201 tcatcccaat actcaccgct ggcaatttcg gctgataatt gccgccagaa tgcgccggca
     1261 tagtaggatt ttccgaactg ccggacatat cttctcaaca agcgatcaat ttcatcgagg
     1321 cggccgacag atatcaggag caagacttgc cgccgcaaag ccgattcttc gatcaaggtg
     1381 cctggagaca gaagtctggc ttggtccaga tagactagtg ctttgtcagg agctgattta
     1441 tcgattaatg ccgcctgcac gagggcgagc gtgccggcag taatcgggtc actctttcgg
     1501 gcgtccatac caaggagaat ttctcgcgcc tcctcgttgc gaccttcgga gtaggcgagc
     1561 gccgctcgtg ccagttcggc ctcaggctta ggtagaccat tgagacttga tatccggcgc
     1621 agaatactgg ggtcgccgcc gctgaaccca tatagggcga cagcgcgcag attgcgcggc
     1681 tccctccaaa cttccggatc gacagcggcc aactgcgccc gaagctttgc catcaattcc
     1741 tgctgagcct tatggtcgat taaatctcct tgcgcgaggg catcctggag atttcgtagc
     1801 tcacgtatca actcgaacgg cgctttggtg cttcctgctg catggggtgc cattgcggct
     1861 gccagcagga gcaccaggat aaaaactatt cctttgagcc ggctcacgat tgctcctcac
     1921 tccggaggag aatttcgatt cggcggtttt gcgccgcttc cggatcgtgg gcgaccctga
     1981 ggctccggtc agcgtgcccc tcgatgcgtt cgaaccgccg ctcatccacg cccccgcgcg
     2041 tcagcaggag ataagccata tgggcgcggg cggtggagag gcgccaatta tcgtaagttt
     2101 gggttcggaa cggcctcccg tcggtgtggc cacgcacgat gagccgtcca ggataatctt
     2161 tcagcacccg ggcgattttc tccatgatga cgatcagctc cggccgcggc tcggcggatg
     2221 cgatcgcaaa cattccgaag tcgaactcat ctgttaggct gatcaggatg ccgtcggcgg
     2281 tccgaaccac ctcgacgtga ggaattttgg ccaaagcggc ttcgttcagc gctcttcgga
     2341 tatcctctgc aacgctgatg tgcgcaggcc gcggttccgg cttctcagtt ggaagaggct
     2401 ccgcgctctc cggactctcg ggcggatttg tctccgccga ctcagattct gccttcactt
     2461 cggaatgaag tgtatcacgc gaagtttcaa gagcagcgtt gtcatcaaaa gtacttggtc
     2521 tttcagaaac cgtatcaacc gccctatctc gatctggttc ctgcggaatt gcttcgctg
//
LOCUS       DASUMG010000563         3282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_961003, whole genome shotgun sequence.
ACCESSION   DASUMG010000563 DASUMG010000000
VERSION     DASUMG010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961003"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VFX46_09425"
     CDS             complement(<1..614)
                     /locus_tag="VFX46_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX5600846.1"
                     /translation="MLVRAPAFRGLLAASAFSILIAAWPAPAAGPQLVAAPSAVPVVK
                     ANAARLPAKPLLTPDRAARESDHVTRFDKAIAPAREHPLSEEDAKRITDAISAYYARD
                     ESRGRALQREITDPIGRKLVEWYRLRAGLADAFQIRAFLEANPAWPDRELLNRKFEEL
                     LFAQGGSAAAIKNAFSEQEPATGLGLAALASAYLVEGDTKRAKDL"
     gene            909..1799
                     /gene="dapA"
                     /locus_tag="VFX46_09430"
     CDS             909..1799
                     /gene="dapA"
                     /locus_tag="VFX46_09430"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX5600847.1"
                     /translation="MFKGSFTALITPFKNGAFDETAYERIIEWQIAQGTHGLVPVGTT
                     GESPTLSHDEHKRVVEVCVETVRKRVPVIAGAGSNSTAEAIDFAKHAKDAGADAVLIV
                     MPYYNKPTQEGLYLHVKAINDAVDIPIVLYNVPSRTVADMSVATMARCARLKNVVGVK
                     DATANLARASLQRLACGPDFVMLSGEDATALGFNAHGGQGCISVTANVAPALCAEFQN
                     ACLAGDFAKALSIQDRLMPLHDALFIETNPGPVKYAASLLGLCSAEVRLPLAPVAEST
                     KQTIEAAMIGAGLLQAKAAE"
     gene            1826..2302
                     /gene="smpB"
                     /locus_tag="VFX46_09435"
     CDS             1826..2302
                     /gene="smpB"
                     /locus_tag="VFX46_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX5600848.1"
                     /translation="MAAKGNGGRRLIAENRRARHDYFIKDTYEAGIALTGTEVKSLRQ
                     GQANIAESYAAFEDGGLTLINAYIPEYKQAGAFFNHEPRRPRRLLMHRRELHKLANAV
                     SRQGMTIVPLELYFNERGIAKLRLGLAQGKKMHDKRETEAKRDWERQKARLMRERG"
     gene            2308..3114
                     /locus_tag="VFX46_09440"
     CDS             2308..3114
                     /locus_tag="VFX46_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX5600849.1"
                     /translation="MPDAAGGGSARCGFADKTAAGFGEDELRAIEVAAVEAWPAFETR
                     NISGWLWRYSGGGSQRANSVSTLEFRGPDAEAAIAEAEALYFSRNVPSRFQVSSTLAE
                     PKDLDRRLERRGYRIHESVTTLAMRLSDHKDIPTGVIIADRPSDEWMEVYLSNVRPDR
                     HAAAPAILAAVPTPKAFFSFEVGGRILSTALAVLRGEIVIAECIGTRAEARRRGAGST
                     VMAALAAWGWQHGARIAALQAVTENFPAQRLYAALGFREVGGYHYRLREP"
BASE COUNT          633 a          965 c         1076 g          608 t
ORIGIN      
        1 agatctttcg cgcgcttggt gtcaccctcg acgagatagg cggaagcaag cgctgccagg
       61 ccgaggcccg tggctggctc ttgctctgaa aaggcgttct tgatcgcggc tgcgcttccc
      121 ccttgcgcaa aaagcagctc ctcgaatttc cggttgagaa gctcgcgatc cggccaggca
      181 gggttggcct cgaggaaagc gcggatctgg aaggcatccg ccaacccggc gcgcagccga
      241 taccactcga ccagcttgcg gccgatcgga tccgtgattt ctctttgcag tgcccgtccc
      301 cgcgactcgt ccctggcgta gtaagcgctg atcgcatcgg taatacgctt tgcgtcctct
      361 tcgctcaggg gatgctcgcg ggcaggcgca atggccttgt cgaagcgagt gacgtgatcg
      421 gactcgcgcg cggctctatc tggcgttagc agcggcttgg caggaagccg cgcagcgttt
      481 gccttgacga ccggaaccgc cgacggcgcc gcaacgagtt gcggaccagc ggcaggagca
      541 ggccacgcag cgatcagaat cgagaaggca gatgccgcga ggagacctcg gaaagcggga
      601 gcgcgaacta gcacgtgcgt atcgtctctc gtcttgaagc tggtggtcgg ttgtcggtca
      661 tcgccgggcg gtaccaagcg ccggccacaa attactccct gacttaagat gaacgggagc
      721 ttcaccttgc gaacacccct tgaagcgcct aatgttccgg ccttaacgaa ggcactatag
      781 gtgcggggct ggggtaccgg aaccacggta tcaagcacgc attccgtgtt caaggacgaa
      841 cgcccggccc gatgccaacg ttcgttagtt gctttcggtt cgggtagccc ctgctcgagg
      901 agcagcacat gtttaaggga tcgttcacgg cgttgattac gccgttcaag aacggcgctt
      961 tcgatgagac ggcctatgag cggatcatcg aatggcaaat cgctcagggt acgcacggcc
     1021 tcgttcctgt cggaacgacc ggcgagagcc ccacactttc ccacgacgag cacaaacggg
     1081 tcgtcgaggt ctgtgtagag actgtccgaa agcgcgtacc agtaattgcg ggtgctggct
     1141 ccaactcaac ggcagaggcg atcgatttcg ccaagcatgc gaaggatgcg ggcgcggacg
     1201 ccgtcctcat cgtcatgcct tactacaaca agccgaccca ggaggggctc tacctccacg
     1261 tgaaggcgat caacgatgcc gtcgacatcc cgatcgttct ctacaacgtt ccgtcgcgca
     1321 cggtagcgga catgtcggtg gcgaccatgg cacgctgcgc tcggctaaag aatgtcgtcg
     1381 gcgtcaagga cgcgacggcg aaccttgcgc gcgcttcgct tcagcggctc gcctgtggtc
     1441 cggacttcgt catgctctcg ggtgaggatg cgacggcgct cgggttcaat gcgcacggcg
     1501 gccaaggctg catctcggtc accgcgaacg ttgcgccggc gctttgcgca gagttccaaa
     1561 atgcctgcct tgccggtgat ttcgcgaagg cgctcagcat tcaggaccgt ttgatgccgc
     1621 tccacgatgc gctgttcatc gaaacgaacc ccgggccggt caagtacgct gcatcgcttc
     1681 tcgggctgtg cagcgctgag gtgcggctgc cgctggctcc ggtcgccgaa tcgacgaagc
     1741 agaccatcga ggcggcgatg atcggtgcag ggctgctgca ggcgaaggcg gccgagtagg
     1801 ggccggcggg gagttctaag acaaaatggc ggccaaaggt aatggcggcc gccggctcat
     1861 tgctgagaac cggagggccc gccacgatta cttcatcaag gatacctacg aggcgggcat
     1921 cgcgctcacc ggcactgagg tgaaatctct gcgtcagggg caggccaaca tcgcggaaag
     1981 ctatgccgca ttcgaagatg gcgggctgac gctgatcaat gcctatattc ccgagtacaa
     2041 gcaggccggc gcattcttca atcacgagcc aaggcggccc aggcggctgc tgatgcatcg
     2101 ccgggagctg cataagctcg cgaatgctgt gagccgccag ggtatgacga tcgtcccgct
     2161 ggagctttat ttcaacgagc gaggtatcgc caagctgagg ctgggactcg ctcaaggcaa
     2221 gaagatgcac gacaagagag aaacggaagc caagcgggac tgggagcgcc agaaggcacg
     2281 tctgatgcgc gagaggggct gagcacaatg ccggatgccg ccgggggagg gtcggcacga
     2341 tgcggcttcg ctgataagac agccgccggg ttcggcgagg acgagttacg ggccatcgag
     2401 gttgccgccg tcgaggcctg gcccgccttc gaaacacgta acatcagcgg ctggctgtgg
     2461 cgctattcgg gcggtggatc tcaaagggcc aactcggttt caactttgga attccgaggt
     2521 ccagacgctg aggctgccat agctgaggcc gaggcgcttt atttcagtcg caatgtgcca
     2581 agtcgctttc aggtgagcag cacgcttgcc gagccaaaag atctcgatcg gaggctcgaa
     2641 cggcgcggat accgcattca tgagtcggtc acgacgttgg ccatgcgctt gtcggaccat
     2701 aaggacatcc cgacaggggt gattatcgcc gaccgtccga gcgacgagtg gatggaggtc
     2761 tacctgtcta acgtgaggcc agaccgtcac gcggccgcgc cggccatttt ggccgcggtg
     2821 cccactccaa aggcgttttt cagcttcgaa gtcggtgggc gaattctcag cacggctctg
     2881 gccgttttac ggggtgaaat cgttatcgcc gaatgtatcg gcacacgcgc cgaggcgcgg
     2941 cggaggggcg ccggcagcac tgttatggct gcgctcgcag cgtggggctg gcagcacggg
     3001 gcgaggatcg cagcgcttca agccgtgacc gaaaactttc cggcgcagcg attgtatgca
     3061 gccctcggat ttcgtgaggt gggcggttat cactataggc tgcgcgagcc ttgaggaggg
     3121 ccgatcgtga cctctcaaac cgactggccg tggcccgcgc ccactgatga tgggcgcgct
     3181 gagcatctaa tccgcggtat ggccgttcct gacatcgcgc tcccgtcgac gggaggccga
     3241 gttgtctccc tcgcccagat ccctggatgg gtggtcgtgt tc
//
LOCUS       DASUMG010000564         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_961155, whole genome shotgun sequence.
ACCESSION   DASUMG010000564 DASUMG010000000
VERSION     DASUMG010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961155"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VFX46_09445"
     CDS             complement(<1..1520)
                     /locus_tag="VFX46_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX5600850.1"
                     /translation="MTSADTTIPTELGRPRSAGAQVLRLALRELRSGISGFYVFIACV
                     ALGVMVITAVGALTDSLRRGFEEHGELLLGGDVVLSRAHRPAEPQERRWLEAQERVAE
                     MATMRTMARRVDGQDQALVELKGVDPAYPLVGSVTLQGGADFAEAIHKKGGAVVAPIL
                     LERLGVKVGDSISIGSISVPITGVVEDEPDNISDNLTYGPRIFLSLDKMRETGLVQLG
                     SLIRWRYALKIANGRASDEQLAQFRDQLEAALPEHGFSVRDRRDPSPRITRSIERLRE
                     FLTLLGLTALLVGGVGVANAVATFIDRRRKVIATFKSLGATSGMIFGVFLTQVMLIAA
                     IGVLIGLVLGFLVPVAANAIYGDALPVSISVIISPWSIVSATAYGLLVALMFTLWPLG
                     RAELVRAGVLFRDEVAPEQVRPRPRVIALTVAAIAALVLLAVLSSDSRWIATYFCLGL
                     TAVFALFTGLGKLIAGLARRAPRSRIPEVALAIGNLGGPGGLTHSTVLSLGAGLSL"
     gene            complement(1517..2221)
                     /locus_tag="VFX46_09450"
     CDS             complement(1517..2221)
                     /locus_tag="VFX46_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600851.1"
                     /translation="MPAPTAPLISLRDVRLTLVSRAGPVEILRGINLEIGAGEAVAIV
                     GPSGSGKTSLLMVMAGLERATSGSVRIAGHDFTKLGEDELALIRGREIGIVFQSFHLV
                     PTMTALENVALPLEFAGNPDAFRIAEEMLAEVGLSHRTDHFPAQLSGGEQQRVALARA
                     LAPKPKILLADEPTGNLDGKTGNQIIELLFDLHRRRGATLVLITHDPALAERCGRTVR
                     MADGRIIADSAEVEAA"
     gene            2368..>2660
                     /locus_tag="VFX46_09455"
     CDS             2368..>2660
                     /locus_tag="VFX46_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX5600852.1"
                     /translation="MLVALVNACLLFVASPGDAQAQSDPIRIVAFGDSLTAGYGLKPS
                     EAFPAQLEKALRDKGYAVEVINAGVSGDTTAAGLERFDWAFAEDVDAAIVALGA"
BASE COUNT          527 a          845 c          840 g          448 t
ORIGIN      
        1 aataatgaca agcccgcgcc aagcgacagg accgttgaat gagtcagccc tccggggcct
       61 cccaagttcc cgatggcaag cgcaacttcg ggaatacgag agcgcggagc acggcgcgcg
      121 agaccagcga tgagctttcc aagccctgtg aagagcgcga ataccgctgt caacccaagg
      181 cagaagtagg tcgcgatcca gcgcgagtcc gacgatagga ctgcaaggag gacgagcgct
      241 gcgatggcgg cgaccgtcag ggcgatcact cgcggccgag ggcggacttg ctcaggcgcc
      301 acctcatccc ggaacaacac tcccgcgcga accagctccg ctcgccccaa tggccacagc
      361 gtaaacatca gagccaccag caaaccgtag gccgtcgcag aaacaatgct ccaggggctg
      421 atgatcaccg agatgctgac gggcagtgca tcgccgtaga tggcgtttgc tgcgacagga
      481 acaagaaaac ccagaacgag gccgatcagc acgccgatgg cagcgatcag catgacctgc
      541 gtgaggaaaa ccccgaagat catgccgctg gtcgcgccga ggctcttgaa cgttgcgatg
      601 accttccgcc ggcggtcgat gaacgtggcg accgcattcg ccacaccaac accaccgacg
      661 agcaatgccg tgaggccaag caacgtcagg aactcgcgaa ggcgctcaat ggaacgggtt
      721 atgcgcgggg atggatcgcg ccggtcccgc accgagaagc cgtgttccgg tagcgccgcc
      781 tcgagctggt cgcgaaactg ggccaactgc tcatcgctcg cacggccatt agcgatcttc
      841 agggcatacc gccagcggat gaggctgccg agctggacga ggcccgtttc ccgcatttta
      901 tcgagggata ggaagatgcg cgggccgtag gtcaggttat cgctgatgtt gtcgggctca
      961 tcttcgacga cgccggtgat cggcacggag atggagccga tcgagatgct gtctccgact
     1021 ttcaccccca ggcgttccag caggatcggc gcgaccacgg cgccgccctt cttgtggatg
     1081 gcttcggcga aatcagcgcc accttggagc gtgacacttc cgacgagcgg ataggcgggg
     1141 tcgaccccct tcagctccac gagcgcctgg tcctggccat ccactcgccg cgccatggtg
     1201 cgcatggtcg ccatctcggc gacgcgctcc tgcgcctcca gccaacgccg ttcctgtggc
     1261 tcggccggcc ggtgtgctcg cgacaggacg acatcgccgc ccagcaacag ctcgccgtgc
     1321 tcctcgaagc cgcgacgcag cgagtcggtc agcgccccaa cggctgtaat caccatcacg
     1381 ccgagggcga cgcaggcgat gaaaacgtaa aagccggaga tgccgctccg aagctccctc
     1441 agagccaagc ggaggacctg cgcccctgcc gatcgtggcc ggccaagttc cgttgggatc
     1501 gttgtgtcag cgctcgtcat gcagcctcga cttccgcgct gtccgctata atccggccat
     1561 cggccatgcg caccgtgcgc ccgcagcgct cagccaatgc cgggtcatgg gtgatgagca
     1621 cgagggtcgc gccacgccgc cggtgcaagt cgaacagcag ctcgatgatt tgattgccgg
     1681 tcttgccatc aagattgccg gtgggctcgt ctgccaaaag aatctttggc ttcggtgcga
     1741 gcgcgcgcgc cagagcaacg cgttgctgct cgccgcccga cagctgcgcg gggaaatggt
     1801 cggtgcggtg cgagagcccc acttcggcca acatttcttc ggcgatccgg aaagcgtcgg
     1861 gatttcccgc gaattccaag ggcagcgcca cgttttccag cgccgtcatg gtcgggacaa
     1921 ggtggaagga ctgaaagaca atcccgattt cgcggccccg aatgagcgcc agctcgtcct
     1981 ctccgagctt agtgaagtcg tggccagcaa ttctcaccga gccgcttgtc gcgcgttcca
     2041 gaccggccat caccatcagc aaagaggtct tgccggatcc ggaagggcca acaattgcga
     2101 ccgcctcacc tgcgccgatc tccaagttta tgccacgcag aatctcgacc ggacctgcac
     2161 ggctgacgag cgtcagcctg acgtcgcgaa gggatatgag gggtgctgta ggtgccggca
     2221 cgcaggcctc cgaaagcttg tgtctgtcga gcgcgctatc tacttggcta tctagtgcgt
     2281 gaggagtctg ttgaccggaa agtgatcggt acgggaggcg atgcgatgag gcactggact
     2341 gcaacccgcg cacttgcaca ggtgagcatg ctcgttgccc tcgtcaacgc ctgcctgctt
     2401 ttcgtcgcta gcccgggaga tgctcaagcg cagtctgatc ccatccgcat cgtcgctttc
     2461 ggtgacagtc tgacagccgg ctatgggctg aagccgagcg aggctttccc agcgcaactc
     2521 gaaaaggcct tgcgggacaa ggggtacgcc gtcgaggtaa tcaatgcagg cgtttcgggc
     2581 gataccacgg cggccggcct ggagcggttc gactgggctt tcgccgagga tgtagatgca
     2641 gcaatcgttg cgctgggcgc
//
LOCUS       DASUMG010000565         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_560416, whole genome shotgun sequence.
ACCESSION   DASUMG010000565 DASUMG010000000
VERSION     DASUMG010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560416"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1386
                     /locus_tag="VFX46_09460"
     CDS             <1..1386
                     /locus_tag="VFX46_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX5600853.1"
                     /translation="TMLNLVAVGLGRDLLTLVGLIAVMVSQDPLLAAICLIGGPFAAL
                     VLKKLTERVQKAATRQFTGMSTIVGTMRESAQGIRVVKSFQLEPLLQRRMNQGVEGVE
                     KLSNKIVAVSALTNPLIETLGGVAVAAAVIYAGWRSLSFGDTPGQFFAFITALLMAAD
                     PARRLSKLQLQLATAAVGVRMMYDLLDTPIAEADEPRRPNLNVSRGEVRFENVTFSYD
                     PDTPVLCDLNLVAPPGKTTALVGLSGAGKTTVFNLLQRFWTPAEGVIRIDGQPISEVS
                     LASLRQQIALVSQDVFLFEGTIRENIVAGRENATEEEVIAAAKAAQAHEFICSLQNGY
                     DTQVGELGSQISGGQRQRISLARAFLKNAPIILLDEPTSALDSETEQSIQTALQELTR
                     GRTTIVIAHRLATVANADVIHVMDKGRVIESGRHRELIERGGLYARLYQIQFANAISE
                     PEDKSIAAAAV"
     gene            complement(1383..>2453)
                     /locus_tag="VFX46_09465"
     CDS             complement(1383..>2453)
                     /locus_tag="VFX46_09465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX5600854.1"
                     /translation="PNSVDLCVHDLVAASRHRDTTCILAEHVHDFFKEFETADVPPIF
                     ANHTALLAHHAARVARRRGTDLIVVHQHFQIAARLARMLPKRVVFHAHGFYKRYSSGH
                     LHSLRRQLRLRELNRLAGLVHVSEACCAHFACAWPEVSLPQAVVYNGFDFGQWTPTRP
                     KQSEILCVGRCVPEKGILEAAQAVARVLAERPEWRARFSLSESERDPAYLQKVQGAIT
                     AAAVSDRVTIELNVPWRGIKDRCEQAAIALVPSRWREPFGRTALEAHAGGAALISSGS
                     GGLREVSGPHALFVDPADIDGFANAIRRLIDDEELRRSLSDGGARYVRERFSIEAVAA
                     AYDAYLEEVWAKLSKRSKCRAD"
BASE COUNT          481 a          762 c          742 g          468 t
ORIGIN      
        1 accatgctga accttgtcgc cgtcggcctt gggcgcgatc tcctcaccct ggtcgggctg
       61 atcgccgtga tggtgtcgca ggatcctctg ctcgccgcaa tttgcctgat aggtggcccg
      121 ttcgccgcgc tggtgctcaa gaagctcaca gaacgggttc agaaagcggc cacgcggcaa
      181 ttcaccggca tgtccacgat cgtcgggacc atgcgggaga gcgcacaggg cattcgcgtc
      241 gtcaaatctt ttcagctcga gcctctgctg cagcggcgta tgaaccaagg tgtcgagggt
      301 gtcgagaagc tgtcgaacaa aatcgtcgcc gtgagcgcgc tgacgaaccc gttgatcgag
      361 acactcggtg gcgtggcggt ggcggcagcg gttatatatg ccggttggcg cagcctatcc
      421 ttcggcgaca cgcctgggca attcttcgcc ttcatcacgg ctttgttgat ggcggccgac
      481 ccggcccgtc ggctgtcgaa gctacaactc caactcgcga cggcagcggt cggtgtacgc
      541 atgatgtacg acctgctcga tacgccgatc gcggaggcgg acgagccccg gcggcccaat
      601 ctgaatgtca gccgcggcga ggttcgattc gagaacgtca ccttttccta cgatcccgat
      661 acgcccgtgt tatgcgatct caatctcgtc gcacctcctg ggaaaactac ggccctcgtt
      721 ggcctttcag gcgccggaaa gacgaccgtc ttcaacctcc tccagagatt ttggacgccg
      781 gcggagggtg taatccgcat tgacggccag ccgatctctg aggtttcgct tgcgtcactt
      841 cggcagcaga tcgcgctggt cagtcaggat gtctttctat tcgaaggcac gatccgcgag
      901 aacatcgtag ccgggcggga gaatgcgacg gaagaggagg tcattgccgc agccaaggct
      961 gcgcaagcgc acgagttcat ctgctccctg cagaacggct acgacacaca ggtcggcgag
     1021 ctcgggtcgc agatttcggg cggccagcgc cagcgtattt ctctggctcg cgcgttcctg
     1081 aagaacgccc cgatcattct cctcgacgag cccacgtcgg ccctagacag cgagacagaa
     1141 cagtcgatcc aaacggctct gcaggagctg acccgcggcc ggacgacgat cgtgattgct
     1201 catcgtttgg ctacggtggc caacgcagac gtcattcacg tgatggacaa aggccgggtg
     1261 atcgaaagcg gtcggcaccg tgagcttatt gagcgcggtg gcctgtatgc ccgtctttac
     1321 caaatccaat tcgcgaacgc gatctccgag cctgaagaca agagcatcgc cgcagcggct
     1381 gtctagtccg ctcggcactt cgatcgtttg ctcaatttgg cccagacctc ttccaggtag
     1441 gcgtcatagg cggcagccac cgcctcgatc gaaaagcgct cgcgaacgta ccgtgcgcca
     1501 ccatcggaga gactccgccg cagctcctca tcatcgatca atcgtcggat cgcattcgcg
     1561 aaaccatcga tatctgccgg atcgacgaag agcgcgtgcg ggccactgac ctctctcagt
     1621 ccgcctgacc ccgacgagat gagggctgcg ccaccggcgt gcgcctcgag tgccgtacgc
     1681 ccgaatggct cacgccagcg ggatgggacc agggcaattg ctgcctgctc gcagcggtcc
     1741 ttgatgccgc gccagggcac attcagctca atcgtcacgc ggtcgctcac cgcagcggcg
     1801 gtgatagccc cttggacctt ttgtaagtag gccggatcgc gttctgactc cgaaagtgag
     1861 aagcgcgcgc gccactcggg ccgctcggcc aggacccggg cgacggcttg ggctgcctca
     1921 aggataccct tctccggaac gcaccggccg acgcacaaaa tctcgctttg ctttgggcgc
     1981 gtcggtgtcc actgaccaaa atcaaaacca ttgtacacga ctgcttgggg cagcgacacc
     2041 tcgggccagg cgcaggcgaa atgcgcgcaa caagcctcac tgacatgcac cagaccagcc
     2101 aggcggttta gttctcgtaa tcgaagctgg cgtcgtaacg aatgcaggtg gccgctggaa
     2161 tagcgcttgt agaaaccatg tgcatggaac accacccgtt tcggcagcat tcgggcgagc
     2221 cgcgccgcga tttgaaaatg ctgatgaaca acgatgagat ctgtccctct ccttcgagcg
     2281 acccgcgccg catggtgcgc aagcagcgcg gtatggttcg cgaatatggg cggaacatct
     2341 gccgtttcga actctttgaa gaaatcgtgc acgtgctcgg ccaggatgca cgtcgtatcg
     2401 cgatggcggc tcgcggcgac gaggtcatga acgcatagat cgaccgagtt cgg
//
LOCUS       DASUMG010000566         3663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_560566, whole genome shotgun sequence.
ACCESSION   DASUMG010000566 DASUMG010000000
VERSION     DASUMG010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560566"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..171
                     /locus_tag="VFX46_09470"
                     /pseudo
     CDS             1..171
                     /locus_tag="VFX46_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            241..1233
                     /locus_tag="VFX46_09475"
     CDS             241..1233
                     /locus_tag="VFX46_09475"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX5600855.1"
                     /translation="MARPDGPPLIKLERLTKHFVVSSGSKLFGGSSAIVRAVNDVSFS
                     IAPGQTLGLVGESGCGKTTVGRLVLKLEEATSGSIIFEGKDLTKAGHDVMRDVRRRIQ
                     VIFQDPYSSLNPRMTVGQIISEPLLVYRMVPNKKAARERVEDLLTQVGLYPYMADRYP
                     HELSGGQRQRVGIARALAMEPKFIVCDEPVSALDVSIQAQVINLLEDLQEKYGLTYLF
                     IAHDLAVVRHISHRIAVMYLGRVMEIADRNSLYENPLHPYTQALLAAVPVPDPAAERA
                     RDHTILSGEVPSPLNPPSGCVFRTRCPRATEECKVVVPELRKFGEDHYAACIHI"
     gene            1279..2859
                     /locus_tag="VFX46_09480"
     CDS             1279..2859
                     /locus_tag="VFX46_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013912826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600856.1"
                     /translation="MSKFAVSAALTAAVALMPAVADAQTPKRGGELRFAVSAEPPDYD
                     CHAATSFAFIHPVRPHYNTLLKFDPANYPKVVGDLAESWEQSEDGLTYTFKLKQNVKF
                     HDGTPLTSEDVKASYERIRNPQGGARSIRQAVYEDIDSIETPDEHTVVFKLKKPNASM
                     LTNFASPWDCIYSAAKLKEDPKFPERNILGTGPFKFVEHAAGSHWVGERFEDYHVEGR
                     PYLDSYRAIFITNTAARVNALQAGEVLSEFRGHSPADRDKLVNALGDKVEVFESPWIC
                     SLVVTFNTEKKPFDDARVRRALSLAIDRWQGAEALSKIALVRHVGGVLRPGFELAATD
                     KDLEQLPGFGRDINAAREEAKKLLAEAGVKDLSFKLMNRNVPMPYTPVGVYLIDQWRQ
                     IGITVEHDQPETAAYISNLRSGNFEAGLDFHCDAVDDPNLQLAKYLSADRSPINYSRA
                     KDAKLDELYEAQKGELNVEKRKQILREFEQHALTEAYNIPVVWWHRIIVNWKQIKGWH
                     MSPSHYLNQDLQDVWLDQ"
     gene            2944..>3663
                     /locus_tag="VFX46_09485"
     CDS             2944..>3663
                     /locus_tag="VFX46_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600857.1"
                     /translation="MLRYTINRLLLMIPTLFGVAVLVFVLLRLMPGDIVELRYLMDGG
                     QVSPEVLAQERARLGLDKPLWEQFVDWITGIFVGDFGVSMWTGRPVWDEISSRFQLSF
                     QVAIMATILATIIAIPLGTLAALFKDTWIDRLIQVFAIAGLAVPSFWLGMLIILGLLL
                     TFNWIPPLTFKPFYEDPWHNLSQLIWPALAVGYRYSAVATRMTRSTILEVLQEDYIRT
                     ARAKGVFERLVVSRHAMRNAML"
BASE COUNT          674 a         1108 c         1137 g          744 t
ORIGIN      
        1 ccgcgggaag ccaagctcga gacgatcgaa gggctgccgc cgaacctggt ttctccgccg
       61 ccggggtgcc gctttgctcc ccgctgtgca gcggcaatcg agtcgtgcaa ggctgctctg
      121 ccaccactcg tcgaggtgga gaccgggcat cattcggcct gcatccgggc caccgagctc
      181 gcggaaaagg gcccacaggc catcggattg tcgacgttcg ccggcccggc caagctcgat
      241 gtggcacgcc ccgatggccc gccgctgatc aagctggagc gtctgacgaa gcactttgtc
      301 gtgtcctcgg gctcgaagct tttcggcggg tcgtcggcga tcgtacgggc ggtgaacgac
      361 gtcagcttct cgatcgcgcc cggacagacg cttggcctcg tcggtgaatc cgggtgcggc
      421 aagaccacgg tgggccggtt ggtcctcaag ctggaggaag cgacttccgg ctcgattatc
      481 ttcgaaggca aggatttgac caaagccggc catgacgtga tgcgcgacgt gcgccggcgc
      541 attcaggtca tcttccagga cccgtattcc tcactcaacc cgcgtatgac cgtcggccag
      601 atcatctcgg agccgctctt ggtctaccgc atggtgccga acaagaaggc cgcgcgggag
      661 cgggtggagg atctgctgac ccaggtcggc ctctatccgt acatggcgga tcgctacccg
      721 catgagcttt caggtggaca gcgccagcgc gtgggtattg cgcgtgccct cgcgatggag
      781 ccgaagttca ttgtttgcga cgagcccgtc tcggcgctcg atgtgtcgat ccaggcgcaa
      841 gtcatcaacc tcctcgagga cctgcaggag aagtacggcc tcacctacct gttcatcgcc
      901 cacgatctgg ctgtggttcg ccatatttct caccggatcg ccgtgatgta tctcggccgc
      961 gtgatggaga tcgcagaccg gaattctctc tacgagaatc ctctgcatcc ctacacgcag
     1021 gcgctattgg cggccgtgcc tgttccggac ccggctgccg agcgggcgcg cgaccatacg
     1081 attctctcag gtgaggtgcc gagccccctc aatccaccct cagggtgtgt gttccgtaca
     1141 cgttgcccca gggcgacgga ggaatgtaag gtcgtcgtgc ctgagttgcg caagttcggg
     1201 gaggaccact acgcggcctg cattcacatc tgatggggga gcgcgttcct gttttctatt
     1261 ttggctggga ggacgttaat gtcgaagttc gcagtatcgg cggctctcac ggcggcggtc
     1321 gcactcatgc ctgccgttgc agatgcccaa acccccaaga ggggcggcga gttgcgcttt
     1381 gctgtcagcg ctgagccgcc tgactatgac tgccacgcgg cgacatcgtt tgcgttcatt
     1441 catccggtgc gcccccatta caacacgctg ctcaagttcg acccggccaa ctatccgaag
     1501 gtggtcggcg atctggcgga gtcctgggag cagtcggagg atggcctaac ctacacgttc
     1561 aagttgaagc agaacgtgaa gtttcacgat ggaactccgt tgacgtcgga ggacgtcaag
     1621 gccagctacg agcgtatccg caatcctcag ggcggggcac gctcgatccg tcaggcggtc
     1681 tatgaggaca tcgactccat cgagacaccc gatgagcata cggtcgtttt caagctgaag
     1741 aagcccaacg catcgatgct caccaacttc gcctcgccgt gggattgcat ctatagcgcg
     1801 gcgaagttga aggaagatcc gaagtttcca gagcgcaaca ttctgggcac cggacccttc
     1861 aagttcgtcg agcatgctgc cggctcgcac tgggtcggtg agcggttcga ggactatcac
     1921 gtcgaagggc gcccctacct cgacagctac cgtgcgatct tcatcaccaa caccgcagcg
     1981 cgcgtgaatg cgttgcaggc gggcgaggtc ctctcagagt tccgcggcca ttcgccggct
     2041 gatcgcgaca agctcgtcaa tgccctgggc gacaaggtcg aggtgtttga gagcccgtgg
     2101 atctgcagcc tcgtcgtgac cttcaacacg gagaagaagc cgttcgatga cgcacgcgtg
     2161 cgccgggcgc tatctctcgc catcgaccgt tggcagggag ccgaggcgct gtccaagatc
     2221 gcgctcgtgc ggcacgtcgg tggcgtgctg aggccgggct tcgagcttgc ggcgaccgac
     2281 aaggacctgg agcagctccc gggctttggg cgtgacatca acgcagctcg cgaggaagcc
     2341 aagaaactct tggcggaagc gggtgtgaag gatctaagct tcaagttgat gaaccggaac
     2401 gtgccgatgc cgtacacgcc ggttggcgtc tatctgatcg accagtggcg gcagatcggc
     2461 atcaccgtcg agcacgacca acccgagacg gctgcctaca tctcgaacct gcgcagcggc
     2521 aacttcgaag ccgggctcga cttccattgc gatgctgttg acgatcccaa cctgcagctc
     2581 gcgaaatacc tctcggcgga tcggtccccg atcaactact cgcgggcgaa ggacgccaag
     2641 ctcgacgagc tgtatgaagc gcagaagggt gagctcaacg tcgagaagcg caagcagatc
     2701 ctgcgcgagt tcgagcaaca cgctttgacg gaggcttaca acattccggt cgtctggtgg
     2761 caccggatca tcgtgaactg gaaacagatc aagggctggc acatgtcgcc gagccattat
     2821 ctgaaccagg atctccagga cgtgtggctc gaccagtaac gaagccaagc ggggcggccg
     2881 aatgcggccg tcccgtgatc tccccaacct gagcttgtag agagggaacc gggcggtccc
     2941 gctatgctgc gctacaccat taaccgtctg cttctgatga taccgacgct gtttggcgtg
     3001 gcggttctgg tgttcgtgct gttgcggctg atgccgggag acattgtcga gctgcgctac
     3061 ctcatggatg gcgggcaggt cagccctgag gtgctggccc aagagcgtgc gcgccttggt
     3121 ctcgataagc cgctttggga gcagttcgtc gactggataa cgggtatttt cgtcggcgat
     3181 ttcggtgttt caatgtggac ggggcggccg gtctgggacg agatctcaag ccgtttccag
     3241 ctttcgtttc aggtggcgat catggccacc attctcgcga ccatcattgc cattccgctc
     3301 ggtacactgg ccgctctgtt caaggacacc tggatcgacc gcctcattca ggtcttcgcc
     3361 attgcaggtc tcgcggtgcc gtccttctgg ctcggcatgc tcatcatcct ggggttgttg
     3421 ctcacgttca actggattcc accgctgaca ttcaagccgt tttacgagga tccgtggcat
     3481 aacctttcgc agctcatctg gcctgccctg gcggtgggct atcgctactc cgctgtcgcc
     3541 acgcgcatga cgcgttcgac catccttgag gtcctacagg aggactatat ccgaaccgca
     3601 cgggccaagg gtgttttcga acggctggtc gtttcgcgcc atgcgatgcg aaacgcaatg
     3661 ttg
//
LOCUS       DASUMG010000567         3770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_479210, whole genome shotgun sequence.
ACCESSION   DASUMG010000567 DASUMG010000000
VERSION     DASUMG010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479210"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /locus_tag="VFX46_09490"
     CDS             complement(<1..1395)
                     /locus_tag="VFX46_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX5600858.1"
                     /translation="MDGARPNTVDPKTLAILRELERKVLWLSTYMIHHANHEVDRGDG
                     LKVGGHQASSASLATLMTALYFHILRPEDRVAVKPHASPVFHAIQYLLGNQTLENLKN
                     VRGFGGAQSYPSRTKDKDDVDFSTGSVGLGVAMTGFASLIQDYVYAKWKPSWPKGRMV
                     ALMGDAEIDEGNVHEALLEFWKHGLTNAWWIVDYNRQSLDSVVSDRLFMKVAGLFENL
                     GWKVVLLKYGKLLQQVFARPGGQDIKRWIDDCPNQLYSALVFQGGPAWRKQLESDLAG
                     CEDALEIVRNHSDTELARLMTNLGGHDLETILETFAAAPTDQPVCFIAYTIKGFGLPF
                     QGHKDNHAGLMTKAQMQEFRASQGINQGEEWDRFAGLSLPPEEIQAFLDRVPFNQRGP
                     RRLSAPRVPVPELPIPEPRGVDSTQAGFGRILDDIAKRGGPLADRIVTTSPDVTVSTN
                     LGPWVNRRALFSMQS"
     gene            complement(1409..2614)
                     /locus_tag="VFX46_09495"
     CDS             complement(1409..2614)
                     /locus_tag="VFX46_09495"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HEX5600859.1"
                     /translation="MSLADIEKAARVLEGSIIATDCDQSRTLSTMLGCEIWLKFENLQ
                     FTGSFKDRGALNRLSALSPEERAVGVIAMSAGNHAQGVAYHASRLGIPATIVMPEGTP
                     FVKIENTRRHGAQVIVGGATLEEAAARARDYCQKHGLTFIHPYDDPLIIAGQGTIALE
                     MLDAVPDLDVLVVPIGGGGLVSGIAVAAKAIKPDIEIIGVQAALYPSMYNRIRGTDLP
                     MRGDTLAEGIAVKAPGVLTEALVRKYVDDIVLVSEREIERAVALLIEIEKTVAEGAGA
                     AGLAAVLSDPQRFQGRKVGLVLTGGNIDTRLLASVLTRELAREGRLSELCIDLADKPG
                     QLARLATLVGDAGANIIEVVHQRVFTDLPAKGTLLRMVIETRDRAHLEDTLKRIRAAG
                     FSAEITSSQ"
     gene            2921..3328
                     /locus_tag="VFX46_09500"
     CDS             2921..3328
                     /locus_tag="VFX46_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600860.1"
                     /translation="MMKSLTIATAALALGGTALFASMPAEAAASAKSSGVIIEARLGG
                     PAHVKFRKPSRVSATGVARGALLPYFLKKAQAQRKAINNWSAKVRLMYGSQYASWSRA
                     NAKSTSCNRLGTAVTCRVSAIPDSPYKRYGLLD"
     gene            3487..>3770
                     /locus_tag="VFX46_09505"
     CDS             3487..>3770
                     /locus_tag="VFX46_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018239961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX5600861.1"
                     /translation="MAKVAWIGLGVMGYPMAGHILKKGGHELVVYNRTAAKAEAWVKE
                     YGAGRTAKTPAEAAADADFVFACVGNDDDLRSVTIGENGAFQTMKKGSIF"
BASE COUNT          691 a         1204 c         1169 g          706 t
ORIGIN      
        1 gctctgcatc gagaagagcg cgcgccggtt cacccacgga ccaaggttcg tggaaaccgt
       61 gacgtccggg gacgtcgtga cgatgcgatc ggcgagcgga ccgcctctct tggcaatatc
      121 atcgaggatg cgcccgaagc ccgcctgcgt ggaatcgacc ccccggggct cggggattgg
      181 cagctccggc acaggcacac gtggagcact caggcgccgc ggcccgcgct gattgaacgg
      241 gacgcgatcg aggaaggcct gaatttcctc cggcggcagg gagagaccgg caaagcgatc
      301 ccactcctcg ccctggttga tgccctggct ggcgcggaac tcctgcatct gcgccttggt
      361 catcaggccg gcgtggttgt ccttgtgccc ctggaacggc aggccgaagc ctttgatcgt
      421 gtaggcgatg aagcagaccg gctgatccgt cggcgcggca gcgaacgtct cgagaatggt
      481 ctcgagatcg tgcccgccga gattggtcat caggcgggcg agctccgtgt ccgaatggtt
      541 gcggacgatc tccaacgcat cctcgcagcc ggcgaggtcg gactcgagtt gcttgcgcca
      601 ggccggcccg ccttggaaca cgagcgccga gtagagctgg ttcgggcagt cgtcgatcca
      661 gcgcttgatg tcctggccac ccggccgcgc gaacacctgc tggagcagct tgccgtactt
      721 caggagcacg accttccagc ctaggttctc gaacagtccc gccaccttca tgaacaggcg
      781 gtccgacacg acggaatcga gactctgccg gttgtagtcg acaatccacc aggcgttggt
      841 caggccgtgc ttccagaact cgagcagcgc ctcgtggacg ttgccctcgt cgatctccgc
      901 atcgcccatg agtgcaacca tccggccctt gggccaggac ggcttccact tggcgtacac
      961 gtagtcctga atgaggctcg cgaagccggt catcgccacg ccgagcccga ccgagcccgt
     1021 cgagaagtcg acgtcgtcct tgtccttcgt gcgcgaaggg tagctctggg cgccaccgaa
     1081 gccgcggacg tttttcagat tctcaagcgt ctgattgccg agcagatact ggatggcatg
     1141 gaacaccggg ctcgcatgcg gcttcaccgc aacccggtcc tcgggccgca ggatgtggaa
     1201 atagagggcg gtcatcagcg tcgcgagcga cgccgaggac gcttgatggc cgccgacctt
     1261 cagcccatca ccgcgatcca cctcgtgatt ggcgtggtgg atcatatagg tggaaagcca
     1321 aagtaccttg cgctccagct cgcgcaggat cgccagcgtc ttggggtcca ccgtgttcgg
     1381 acgggcgcca tccataagca accatctcct attggctcga cgtgatctcc gccgagaagc
     1441 cggcggcacg aatgcgcttg agtgtatcct ccaggtgggc acggtcgcgc gtctcgatca
     1501 ccatgcgcag aagcgtgccc tttgcaggaa gatctgtgaa aacgcgttga tgcacaacct
     1561 cgatgatgtt tgcgccggca tcccccacca gagtagcgag tcgcgcgagt tggccgggct
     1621 tgtcggccag atcgatgcac agctctgaaa ggcgaccttc acgggccagc tcacgggtta
     1681 ggacgctggc gaggagccgc gtgtcgatgt tgccgcctgt caggacaagc ccgactttcc
     1741 ggccctggaa ccgctgcggg tcgctaagca cggcggccag ccctgcggcg cccgcgcctt
     1801 cggcgacggt tttctcgatt tcgatcagca gtgcgacggc gcgctcgatc tcccgctcac
     1861 tgaccagaac aatgtcatcc acgtatttcc gaaccagcgc ttccgtcagc acgcccggag
     1921 ccttcactgc gatgccttcg gcaagcgtgt cgccgcgcat gggcaggtcg gtgccacgaa
     1981 ttcggttgta catcgatgga tagagcgcgg cttgcacgcc gatgatctcg atgtcgggct
     2041 tgatggcctt cgccgcgacg gcaatacctg agacgagccc gccaccgcca atcggaacga
     2101 cgagaacgtc gagatccggc accgcgtcga gcatttcgag cgctatggtg ccctgtccag
     2161 cgatgatcag cgggtcgtca taggggtgga tgaacgtcag cccgtgcttc tggcagtagt
     2221 cgcgggcgcg agccgccgcc tcttccagtg tagccccgcc cacgatcacc tgcgctccat
     2281 gccggcgcgt gttctcgatt ttcacgaacg gcgtaccctc cggcatcacg atggtcgccg
     2341 gaatgccgag ccggctcgca tggtaagcca caccttgtgc gtggttgccg gcggacatgg
     2401 cgatgacgcc caccgcccgc tcttcgggag agagagcact gagacggttc agcgcgcccc
     2461 gatccttgaa agagcccgtg aactgcagat tttcgaactt cagccagatc tcgcatccga
     2521 gcatcgtcga gagcgtacgg ctctgatcgc aatcggtcgc gataatcgag ccttcgagca
     2581 cccgcgcagc cttctcgatg tcagcgaggc tcaccgctcc tgctggtgcc gcagcgctct
     2641 ctcggggctc tgccgaatcc ccctgcgtcg ctttcgtctt ccttggtgac atatccaggc
     2701 gtcgatggtt cgagcgtcgg cctctggtcg ccgctttagg cgtgtttcag cgccgctgct
     2761 tagcaccctc tgacatctcg cgaaacagtc cgcgccaatg aacgctcgtt gaacgatccg
     2821 ttcagcatca cctcaggcgc tcgactctaa tttcggtctc gtcaacgggg ccaagcgcac
     2881 agtgcgcggc tcgaggcaaa caacaggatg gagattgatc atgatgaagt cgctgacaat
     2941 cgcaacagca gctctcgcgc tcggtggaac ggctctcttt gcctcgatgc cggccgaggc
     3001 tgccgctagt gccaagagtt cgggcgtgat catcgaggcg cgcctcggtg ggcccgcgca
     3061 cgtcaagttc cgtaagccga gccgcgtgtc tgccaccggt gtcgcacggg gcgctctgct
     3121 cccgtatttc ttgaagaagg cgcaggcgca gcggaaagcc atcaataact ggagcgccaa
     3181 ggtgcgtctg atgtatggct ctcaatacgc gagctggtct cgtgccaacg caaagtcaac
     3241 gagctgcaat cgcctcggta cggctgtgac ctgccgcgtc agcgccattc ctgactcgcc
     3301 ctataagcgc tacggtctgc tggactaaac gaagagcaaa gtggtcgcgt acacctcaaa
     3361 ggggagccct caagctccct tttctttttt ggctagccgt ctcccatctc ccgggcctga
     3421 caatatcctc ggcatgggct agaaccctcg ctcaatccca ccacaagaac ccatggagga
     3481 ggcaagatgg caaaggttgc atggatcggg ctcggcgtca tgggctatcc catggccggt
     3541 cacatcctga agaagggcgg tcacgagctc gtggtctaca accggaccgc ggcaaaggcc
     3601 gaagcgtggg tgaaggaata cggcgctggc cggaccgcga agacgccagc ggaagcggcc
     3661 gccgatgcgg acttcgtgtt cgcctgtgtt ggcaacgatg acgatctgcg gtcggtcacg
     3721 atcggcgaga acggcgcctt tcagacgatg aagaagggct ccatcttcat
//
LOCUS       DASUMG010000568         2519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_479291, whole genome shotgun sequence.
ACCESSION   DASUMG010000568 DASUMG010000000
VERSION     DASUMG010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479291"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VFX46_09510"
     CDS             <1..851
                     /locus_tag="VFX46_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX5600862.1"
                     /translation="WIAASPKARQAKSKARYTRYEELLRQASEKQTQTAQIIIPVAER
                     LGQNVVDFEGLSKAFGDRVLIDDLTFKLPPGGIVGVIGPNGAGKTTLFRMITGQEKPD
                     AGTIRLGDTVKLGYVDQSRDALDPNKTVWEEISEGKDVIQLGKREVPSRAYVGWFNFR
                     GPDQQKKVGLLSGGERNRVHLAKMLKEGSNLLLLDEPTNDLDTETLSALESALEDFPG
                     CAVVISHDRFFLDRIATHILAFEGDSHVEWFEGNFEAYEEDKKRRLGDAAVEPHRIKF
                     KRFERT"
     gene            980..1888
                     /locus_tag="VFX46_09515"
     CDS             980..1888
                     /locus_tag="VFX46_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003292671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX5600863.1"
                     /translation="MDAAAGDRRVFLAVVVNVGLTVGQIIGGLLAGSLALIADAVHNF
                     SDAISLGIAFFARKIARRQSDERMTFGYVRAELVAALINYTTLIAIGVYLAYEAILRF
                     FSPQEVEGWLVVAVAGLALVIDAVTALLTYSLAKTSANIRAAFLHNVADALGSIAVII
                     AGALILTFGWHIVDPIVTLIIAAYILWMAFTEVGSVVRILMLGVPPEIDVEELIAAIR
                     KVDGVEDIHHVHVWAIDENRNSLEAHVVVPDDMASDSKAVKDRIRTLVRERFGVTHTT
                     LEIERASECHEVGDAKPIGHHIGHQH"
     gene            complement(1932..>2519)
                     /locus_tag="VFX46_09520"
     CDS             complement(1932..>2519)
                     /locus_tag="VFX46_09520"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX5600864.1"
                     /translation="PRLAISRSRHSLLALLSALTLLLVAPSARASDTDAIEFMKQTAR
                     ELIAAARTGSQRDFMEVIQNRGHIPAIGLYALGNYKSQLRPSDRETYYGGIVRFIARY
                     AATEAPKYPVSHAEILSPVVREGDAIYVDSRVHLRDGSVYDVRWLLIPQGKSFRVRDA
                     QVMGFWMSPFLKNLFESYIGENGGNPQALVIALSR"
BASE COUNT          491 a          783 c          770 g          475 t
ORIGIN      
        1 aatggatcgc agcctccccc aaggcacgtc aggccaagtc gaaggcacgc tacacgcgct
       61 atgaggagtt gctcaggcag gcgagcgaga agcagaccca gacggcgcag atcatcattc
      121 ccgtcgccga gcggctcggc cagaacgtcg tcgatttcga gggcctcagc aaagccttcg
      181 gcgaccgcgt gctgatcgac gatctcacct tcaagctgcc gcccggcggc atcgtcggcg
      241 tgatcggccc caacggcgcc ggtaagacta cgctgttccg catgatcacc ggccaggaaa
      301 agccggatgc tggtacaatc cggctcggtg atacggtgaa gctcggatac gtggatcagt
      361 cgcgcgacgc cctcgacccc aacaaaacgg tctgggaaga gatctcggaa ggcaaggacg
      421 tcatccagct tggcaagcgc gaggtgccgt ctcgggcata tgtcggctgg ttcaatttcc
      481 gtgggccgga ccagcagaag aaagtcggct tgctatctgg cggtgagcgt aatcgcgtgc
      541 atctcgccaa gatgctgaag gaaggcagca acctgctgct cctcgacgag ccgaccaacg
      601 acctcgatac tgagacactg tcggccctcg agtcggcgct cgaggacttc ccgggctgtg
      661 cggtggtcat cagccacgac cgcttcttcc tcgatcgtat cgccacgcac attctggcct
      721 tcgaaggcga cagccacgtc gagtggttcg agggcaactt cgaggcctac gaggaagaca
      781 agaagcgccg cctcggcgac gctgcagtcg agccgcaccg gatcaagttc aagcggttcg
      841 agcggacgta agtcactccc gccgggccag ccccggcggg tgccggaacc tgctttctca
      901 gcgcctgttc cggcttgata tcggccggtg aggcagtatg gcaggtcacg cgcattccca
      961 cagccacagg cacgtgcaca tggacgcggc ggccggggac cgccgtgtct ttctcgccgt
     1021 agtggtcaat gtcggcctca ctgtcgggca gatcatcggc gggcttttgg ccggcagcct
     1081 cgcgctgatt gccgatgccg ttcacaactt ctccgatgcc atctcgctcg gcatcgcttt
     1141 cttcgcccgc aagattgccc gccgccagag cgatgagcgc atgaccttcg gctacgtgcg
     1201 cgccgagctc gttgcagcgc tgatcaacta cacgacgctc atcgccatcg gcgtttatct
     1261 ggcctacgaa gccatccttc ggttcttttc accgcaggag gtcgagggtt ggctggtcgt
     1321 ggccgtcgcc ggtctcgcgc tggtgattga tgctgtgacc gcgctactga cctactccct
     1381 ggccaagacg agtgcgaaca ttcgcgcggc gttccttcac aatgtcgccg atgccctcgg
     1441 ttcgatcgct gtgatcatcg ccggcgcgct gatcctgact ttcggctggc acatcgtcga
     1501 tccgatcgtg acgctgataa tcgccgccta catcctttgg atggcattca cggaggtcgg
     1561 cagcgttgtg cgcattctca tgctcggcgt tccgcccgag atcgacgtcg aggagcttat
     1621 agcggctatt cgcaaggtgg acggcgttga ggacatccat cacgtccatg tctgggccat
     1681 cgacgaaaac cgaaactcgc tcgaggcgca cgtcgtcgtg ccggatgaca tggcgtccga
     1741 ttccaaagca gtgaaagatc ggatcaggac gctcgtccgc gaacgctttg gcgtgacgca
     1801 caccacactc gagatcgagc gcgcctcaga gtgtcatgag gttggggacg caaagccaat
     1861 cgggcaccac atcggccatc agcactgaac gggattggcc tttatgccgc cgcaactggc
     1921 ttccgcgcca actaacgact gagcgcaatc accagcgcct ggggattgcc accgttctcg
     1981 ccgatatagc tttcgaacag gttcttaagg aatgggctca tccaaaagcc catgacctgc
     2041 gcgtcgcgga cacggaatga cttgccttgt gggatcagca accagcggac atcgtacacg
     2101 gacccgtccc gcaagtgcac gcggctgtca acgtaaattg cgtcaccttc gcggacgacg
     2161 ggagatagaa tctcagcgtg gctgacggga tacttcggcg cctccgtggc cgcgtagcgg
     2221 gctatgaagc ggacaatgcc accgtagtaa gtctccctat ccgatggccg caactgggat
     2281 ttgtagttgc cgagcgcata gagcccgatc gcaggaatgt gaccgcggtt ttggatcact
     2341 tccatgaagt cccgctgcga tcccgtacgc gcagcggcga tgagctcgcg cgcggtctgc
     2401 ttcatgaact cgatggcgtc ggtgtccgag gcgcgcgccg aaggtgccac gagcaagagc
     2461 gtgagggcag ataacagtgc taacaagctg tgccggctgc gagaaatcgc caaacgcgg
//
LOCUS       DASUMG010000569         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1043639, whole genome shotgun sequence.
ACCESSION   DASUMG010000569 DASUMG010000000
VERSION     DASUMG010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1043639"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFX46_09525"
     CDS             <1..206
                     /locus_tag="VFX46_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX5600865.1"
                     /translation="LQPVALILPSTYVFEGLRGLVLEQTFHGDFMLRALFLNLVYVLG
                     ALLLFRALLESARRNGALVQLGE"
     gene            255..863
                     /locus_tag="VFX46_09530"
     CDS             255..863
                     /locus_tag="VFX46_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX5600866.1"
                     /translation="MRLRTLGLIAAGLAGGALLALAVVPGAIDKLQAPAQVLSIGKAT
                     VGGPFTLTDHTGRRVTDKDFRGQHLLVFFGFTYCPDVCPSGLQVMTAALEELGPKAEK
                     ITPIFISVDPERDTPEQLALYVSSFHPRLVGLTGTPEEIQQVAKAYRVYYKKVEDESS
                     TAGYTIDHTSIIYLMGPRGEFISHFTHATPASVMVEQLEKVL"
     gene            1012..>2062
                     /gene="phaZ"
                     /locus_tag="VFX46_09535"
     CDS             1012..>2062
                     /gene="phaZ"
                     /locus_tag="VFX46_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEX5600867.1"
                     /translation="MLYHWYELSHAAVRPARVMADAGRLFFNNPLNPLTHTGFGRHAA
                     AACEVFERTTRRYGKPEFRIEETTVDGQPVAVTEEVVWQRPFCRLLHFVRDLPKERRA
                     QDPRILLVAPMSGHFATLLRGTVQTFLPNHEVYITDWQDARDVPLSAGHFDLDDYVDY
                     MRDIFRYFGGDVHVFAVCQPSVPVLIAISLMEEEDDPSIPLSMILAGGPIDTRVNPTV
                     VNKLAEERGTDWFRHNVITSVPWPRLGFGRRVYPGFLQLTGFMTMNLDRHLKAHKELF
                     EHLVRGDGDSAEKHREFYDEYLAVMDLTAEFYLQTVDTVFVRHALPKGEMTHRAERID
                     TSAIRNVALFTVEGEN"
BASE COUNT          386 a          669 c          604 g          403 t
ORIGIN      
        1 ggctgcagcc ggtggcgctc atcctgccat cgacctacgt gttcgaaggc cttcgcgggc
       61 tggttctcga acagacgttc cacggcgact ttatgctacg cgcgctgttc ctcaatctgg
      121 tctatgtatt gggtgcattg ctgctgtttc gagccctgct cgaaagcgca cgccgaaacg
      181 gcgcgctcgt gcagctcggt gaatgaccgg gactgaagaa acacgcaccc cgccaactac
      241 ggagtgagcc tgtcatgcga ctgcgtacgc ttggcctcat tgctgctggg cttgcaggag
      301 gcgcgcttct cgcccttgcc gtagtgcctg gcgccatcga caagctgcag gcacctgcac
      361 aggtgctgtc catcggcaag gccaccgtcg gtgggccatt cacgctgact gaccataccg
      421 gccgccgcgt gacggacaag gatttccgcg gccagcattt gctggttttc ttcggcttca
      481 cgtactgtcc agacgtctgc ccctcgggcc tgcaggtgat gaccgctgca ctcgaggagc
      541 tcggtccgaa agccgaaaag atcacgccga tcttcatcag cgtcgacccg gagcgcgaca
      601 cgccggagca gctcgctctc tatgtgtcga gcttccatcc gcgcctcgtc gggctgacag
      661 gaacccctga agaaattcag caggtcgcga aggcctatcg cgtctactac aagaaggtgg
      721 aggacgagag ctcaacggcc ggctacacga tcgatcacac gtccatcatt tacctcatgg
      781 gccccagagg tgagttcatc tcacacttca cccacgcgac gcctgcctcg gtgatggtag
      841 agcaactcga aaaagtgttg taactgcttc acatcttcca tccggtttcg aatcggtgca
      901 aactggagcg atcgggtggc gcgcaatgcg acttcctgac acttcgagcc tgccgccaga
      961 gcgggccgga atagaaccag gacaggggac aagaaaacgg gggtgtcctg catgctctac
     1021 cactggtacg agctgagcca cgctgccgtg cggccggctc gggttatggc tgatgcaggg
     1081 cgcctgttct tcaacaaccc gctcaatccg ctgacgcata cgggctttgg ccgccacgcc
     1141 gctgcggctt gcgaagtctt cgagcgaacg acgcgccgct acggcaagcc ggaattccgc
     1201 atcgaagaga caacggtcga cggccaacca gtcgccgtta ccgaggaggt agtctggcag
     1261 cgcccttttt gccgcctcct ccatttcgtt cgcgatctgc cgaaagagcg gcgcgcccag
     1321 gacccgcgca ttctgctggt cgcccccatg tccggccact tcgcgacact gcttcggggc
     1381 acggtccaga ccttcctccc aaaccatgag gtctacatca ccgactggca ggacgcgcgc
     1441 gacgtccccc tgtccgcggg ccacttcgac ctcgatgact acgtcgacta catgcgggac
     1501 atcttccgct atttcggcgg cgacgtgcac gtgttcgcgg tgtgccaacc gtctgtgccg
     1561 gtattgatcg cgatctcgct gatggaggag gaggatgacc cctccattcc gctgagcatg
     1621 atcctcgccg gcggcccgat cgacacgcgc gtcaatccga cggtcgtcaa caagctcgcc
     1681 gaggagcgcg gcacagactg gttccgccac aacgtcatca caagcgtccc ctggccgaga
     1741 cttggtttcg gacgccgggt ctatcctggc ttcctgcagc tcacggggtt catgaccatg
     1801 aacctcgacc gtcatctgaa ggcgcacaaa gagctgttcg agcacctcgt ccgcggcgat
     1861 ggtgattccg ccgagaagca tcgcgagttc tacgacgagt atctcgctgt gatggatctg
     1921 acggcggagt tctacctgca gaccgtcgac accgtcttcg tccgtcatgc cttgcccaag
     1981 ggcgagatga cgcatcgcgc tgaacggatc gatacgtcgg ccatacgcaa tgtcgccctg
     2041 ttcacggtcg agggcgagaa cg
//
LOCUS       DASUMG010000570         2941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_1123109, whole genome shotgun sequence.
ACCESSION   DASUMG010000570 DASUMG010000000
VERSION     DASUMG010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123109"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1056)
                     /locus_tag="VFX46_09540"
     CDS             complement(58..1056)
                     /locus_tag="VFX46_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEX5600868.1"
                     /translation="MSNCQFDVVGIGNAIVDIIGQCDDDFLARHNAPKGHMRLIDFDT
                     ATRLYADMGPAIEISGGSVANTMVGIASLGGRAAFIGKVAQDEFGRIFAHDIRSAGVH
                     FETAPSSSGLPTARSLILVTPDGERTMNTYLGVSPYLNRGEVDPEVIRAAGIVFLEGY
                     LFDRPEAKDAFRQAAEIAAAAGRKVALSLSDKFCVDRHREEFLALVRNNIDILIANES
                     EITALYQVEMFDDAVPLAQADTQLAVLTRSAKGSVICAHGQEPIQIQAQRVDNVVDVT
                     GAGDLYAAGFLHGLAAGLSLEVAGRLGSLAAAEVISHFGARPQQSLAALAKAHNLI"
     gene            complement(1113..2162)
                     /gene="holA"
                     /locus_tag="VFX46_09545"
     CDS             complement(1113..2162)
                     /gene="holA"
                     /locus_tag="VFX46_09545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX5600869.1"
                     /translation="MTAIKAQQAEQFLKTLDLRYRAILLYGTDAGVIAERARTAAKKL
                     AESSDPPGEILRIEDADLELEPDRLLVESQTIPMFGGPKVIHTRASRRVNAQVLQPLL
                     QNGALAGALVVEAGNLRPDDSLRVLFEKSPNAAAVACYADETRTLDSIISETLEQAGV
                     TITREAREALIARLGADRALTRGELEKLVLYTRSKRQITIDDVDAIVGDASELAIETV
                     VLAAASGDARRAVQEFDRVVASGESPQAVISAVQRHMQRLHRLRASLERGQSMDAALR
                     QLRPPIHFKTRPHLEAQCRIWTIEKLNTSFSHIADAAKEARLNSAVEASIAERLLLDI
                     AHTAKSDASARSTQA"
     gene            complement(2214..2711)
                     /gene="lptE"
                     /locus_tag="VFX46_09550"
     CDS             complement(2214..2711)
                     /gene="lptE"
                     /locus_tag="VFX46_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX5600870.1"
                     /translation="MAVVMLLAPTLAACEGSTGFRPMYGAATLADTEAKLASVSFGPI
                     PGRVGQKIRNELIFHSTGGGSPLPPKYHVDIAIRQQIVSTLVRKDGESQSQVYNLEAK
                     FQLIRIADNAVLLEGTSYGRASFERYDSIYSNVKAREDAENRAARAVAQDIKTRLAAF
                     LSGTA"
BASE COUNT          577 a          867 c          910 g          587 t
ORIGIN      
        1 aaaatcgagc gccaactgag gatggcacca agcgcgtacg tatggtttgc cggaaaatca
       61 gatgaggtta tgcgccttgg ccaaggccgc caggctttgc tggggtcgtg ccccaaaatg
      121 gctgatgacc tccgcggcgg ccaggcttcc taggcgtccc gcgacttcca ggctaaggcc
      181 tgcagcgagg ccgtggagga agcccgcagc gtagaggtcg cccgcgccag tgacgtcaac
      241 gacgttgtca acgcgttgag cctgaatttg gatcggctcc tgaccgtgcg cgcaaatcac
      301 ggatccctta gcgctgcggg taagcacagc caattgcgta tcagcctggg caagcgggac
      361 cgcatcatcg aacatctcaa cctgataaag cgccgtgatc tcgctttcgt tggcaatcag
      421 aatgtcgatg ttgtttcgga cgagagcgag aaactcctcg cggtggcggt cgacgcaaaa
      481 cttgtctgaa aggctgaggg ctacttttcg tccggcagct gcagcaatct cggccgcctg
      541 acgaaatgcg tccttcgctt cagggcggtc gaaaagatag ccttccagga aaacgatgcc
      601 ggcggcgcgg atcacctcag ggtcaacctc tcctcgatta aggtaagggc tgacgccgag
      661 ataggtgttc atcgtccgct ccccgtcggg agtgacgaga atgagcgatc gggctgttgg
      721 caggccggag gaggacggcg ctgtctcgaa atggacgcca gccgatcgaa tatcgtgtgc
      781 gaagatacgg ccgaattcgt cttgggcgac cttgccgatg aatgcagcgc gaccgcccag
      841 cgaggcaatg ccgaccatcg tgttggcgac tgagccaccg gagatctcta tggcggggcc
      901 catatcggcg tagaggcgag tcgcggtgtc gaaatctatg agccgcatgt gccctttcgg
      961 ggcgttatga cgggccagga aatcgtcatc gcattggcca atgatgtcga cgatcgcgtt
     1021 gccgattccg acgacgtcaa actggcaatt gctcatgtgc tcccccgcag atcatcgggc
     1081 gcactatatg caggagcggc cgcgctgcaa ccttacgctt gtgtactgcg ggcggatgcg
     1141 tcgctcttcg ctgtgtgggc gatatccagg aggagccgtt cggcaatgga agcttccacg
     1201 gccgagttga ggcgcgcttc cttggcagca tctgcaatgt gcgaaaatga ggtgttcagc
     1261 ttctcgatgg tccaaatccg gcattgggcc tcgagatggg ggcgggtctt gaaatggatc
     1321 ggtggccgga gctgccgtaa agcggcatcc atggactgac cgcgttcaag gctcgctcgc
     1381 agccgatgaa ggcgttgcat atgccgttgt accgccgaga tgaccgcctg cgggctctcg
     1441 ccggatgcaa caacccggtc gaactcctga acggcgcggc gtgcgtctcc ggacgcagcg
     1501 gccaggacca cagtttcgat tgccagctcg gaagcgtcgc caacgatggc atcgacatca
     1561 tcaatggtga tctggcgttt gctgcgcgta tacaacacga gcttctcgag ctcaccgcgg
     1621 gtaagggcgc gatcggcgcc tagacgcgcg ataagagcct cccgcgcttc ccgcgtgatg
     1681 gtgacgccgg cctgctctag ggtttcgctg atgatcgaat ccagagtgcg ggtctcatcg
     1741 gcataacagg caaccgccgc cgcgttgggg gatttctcga aaagcactct aagagagtcg
     1801 tcgggacgga gattgcctgc ttcgaccacc aaggctcccg cgagggcacc attctgcaat
     1861 aatggctgga gcacctgcgc attcaccctg cgactggcgc gggtatgaat gactttcgga
     1921 ccgccgaaca tgggaatcgt ctgtgattcg acaagaaggc ggtccggttc gagttcgagg
     1981 tctgcgtcct cgatgcgaag gatttcgccc ggcgggtcgc tggattcagc aagtttcttg
     2041 gctgctgtcc gagccctttc cgcaatgaca cctgcgtcag tgccgtacag caggatggca
     2101 cggtaccgta ggtcgagtgt cttcagaaat tgttcggcct gctgagcttt gatggccgtc
     2161 atacgatctc gtcgttggcg gctacgaccg ccagggtcat gaccagcccg cccttaagct
     2221 gttccagaga ggaaggcggc gagccgagtc ttgatgtcct gcgccaccgc gcgtgccgca
     2281 cgattctcgg catcctcgcg ggccttcacg ttcgagtaaa tcgagtcgta ccgctcgaag
     2341 ctggcccggc cgtagctagt gccttccaac agcacagcgt tgtcggcaat gcgaatcagc
     2401 tgaaacttgg cttcgagatt ataaacttgg ctctgcgact caccatcctt gcgcaccagc
     2461 gtcgaaacga tttgctggcg gatagcgatg tcgacatggt acttcggagg cagaggcgag
     2521 ccgccgcctg tcgaatggaa gatcagctca ttgcggatct tctggccaac gcggccggga
     2581 atcggtccga acgagacaga ggcaagcttc gcttcggtat cggcgagagt ggcagcgccg
     2641 tacatggggc ggaagccggt actaccctcg caggcagcca gcgtcggcgc aagaagcatc
     2701 acgaccgcca tccagcgcgc cgtaccgccc caagtccatg atttcgaagc tgaggagcct
     2761 tgccccctag gcaaccacat tcacgatcct ctgcggcacg acgatcacct tcttcaccgg
     2821 ccgcccgccc agagctcgga taaccggctc cagtttcaag gctgctgcct cgatttcggc
     2881 tttgctcgcg cttctggcga tcgtcagctc gtccctacgc ttaccattca cctgtacggc
     2941 a
//
LOCUS       DASUMG010000571         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_480137, whole genome shotgun sequence.
ACCESSION   DASUMG010000571 DASUMG010000000
VERSION     DASUMG010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480137"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFX46_09555"
     CDS             <1..365
                     /locus_tag="VFX46_09555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX5600871.1"
                     /translation="RFLIFYLACGVVGGLTHTLMMPTSELPLIGASGAVAGVIAAYLM
                     LHPRVRIWVLVMRVIPIRISAAFALGAWIVTQLIMVLLPQVGPVAWWAHIGGLAAGAV
                     LVVFMRRSGVPLFDRGIA"
     gene            589..1344
                     /locus_tag="VFX46_09560"
     CDS             589..1344
                     /locus_tag="VFX46_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX5600872.1"
                     /translation="MKVLVPVKRVVDFNVKIRVKPDGSGVELANVKMSMNPFDEIAVE
                     EAIRMKEKGIASEIVAVSIGPSKAQETIRTALAMGADRGILIETDEGVTVEPLAVAKL
                     LKAVVQEEQPNLVILGKQAIDDDCNQTGQMLAALLGWPQATFASKVVIEDGAARVTRE
                     VDGGLETLKLKLPAVVTTDLRLNEPRYASLPNIMKAKKKPLDVKKVADLGVDIAPRLK
                     VLQTAEPPARKAGVKVSSVAELVEKLKTEAGVL"
     gene            1344..2288
                     /locus_tag="VFX46_09565"
     CDS             1344..2288
                     /locus_tag="VFX46_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX5600873.1"
                     /translation="MAVLVLAEHTNDTFNPVTAKAVSAAAALGGEVHVLVAGSNCRPA
                     AEGAAKIEGVSKVLVADAPHLANQLAEEIAALIVPLMANYDALVAPSTARGKNVLPRV
                     AAMLDVMQISDITAVKSPDTFERPIYAGNAIQTVQSTEPKKVITVRTAAFAAAPEGGS
                     ATIESISAPEPVGLSTFEGAELAKSERPELTAARIVISGGRGLQSAENFKKYIEPVAD
                     RLGAAMGASRAAVDAGFAPNDLQVGQTGKVVAPELYIAVGISGAIQHLAGMKDSKVIV
                     AINKDGEAPIFQVADYGLVADLFQALPELDAELQKAGY"
     gene            2350..3243
                     /locus_tag="VFX46_09570"
     CDS             2350..3243
                     /locus_tag="VFX46_09570"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX5600874.1"
                     /translation="MPGANTVAIKKVGVIGAGQMGSGIAHVVALAGYDVLITDISKEV
                     VDKAMATIGRNMDRQIARGIITEADKQAALGRIAYAPDYDAFSDCDLVIEAATEDESI
                     KRKIFTELSPKVKPEAIIASNTSSISITRLASITKRPERFIGMHFMNPVPVMQLVELI
                     RGIATEDDTFAISREFVESLGKTIAVSEDFPAFIVNRILLPMINEAVYTLYEGVGTVE
                     AIDKAMRLGANHPMGPLELADFIGLDTCLSVMQVLYEGLADSKYRPCPLLVKYVEAGW
                     LGRKTQRGFYDYRGETPVPTR"
BASE COUNT          731 a         1031 c         1170 g          717 t
ORIGIN      
        1 ttcgctttct catattttat ttggcttgcg gggttgttgg cgggctcacc cacacgctga
       61 tgatgccgac ttccgagctg ccgctcattg gcgccagtgg tgccgtagcg ggtgtaattg
      121 cggcctattt gatgttgcat ccccgtgtcc gcatttgggt gctggtgatg cgggtcattc
      181 cgatccgaat ctctgcagcc ttcgcgctcg gcgcatggat cgtgactcag ctcatcatgg
      241 tgttgttgcc ccaggttgga cccgttgcct ggtgggcgca catcggcggt cttgcggccg
      301 gagccgttct agtcgttttc atgcgccggt ccggcgtgcc gctgttcgat cgcggaatcg
      361 catagctgca tcgacgagaa cgaataagtg cggaacatcg tgccaaccga atgattgact
      421 tgccgaaaac gtgagcatac ggtgcctttt cgcacgtgcg aaggattgca gactgcgccg
      481 ggctggtttc ggggattgag acgcttgatc ccagaatggg ccatttttta cggctcgcgt
      541 gcagcacact cgcatgcgat cacaggatga ttgggagaac tcgggatcat gaaagtcctc
      601 gtgccggtca agcgcgtggt tgatttcaat gtgaaaatcc gggtgaagcc ggacggctcg
      661 ggggtagagc tcgccaacgt caagatgtcg atgaacccct tcgacgagat cgcagtcgaa
      721 gaggcgatcc gaatgaagga gaagggcatc gcgagcgaga tcgtcgccgt atcgatcggg
      781 cccagcaagg cgcaggagac gatccgcacg gcgttggcca tgggtgcgga tcgcggcatt
      841 ctcatcgaga cggacgaggg tgtgacggtc gagcctctgg ccgtggccaa gctgctgaag
      901 gctgtggttc aagaggagca gcccaatctc gtcatactcg gcaagcaggc catcgacgac
      961 gactgcaatc agacaggtca gatgttggca gcgttgctcg gatggcccca ggcgacattc
     1021 gcctccaagg ttgtgatcga ggatggcgct gcccgagtga cgcgtgaggt cgatggcggc
     1081 ctggagacgc tcaagctgaa gcttccagcg gtggtgacaa cggatctgcg gctcaacgag
     1141 ccgcgctacg cctctctgcc caacatcatg aaggcgaaga agaagccgct ggatgtgaag
     1201 aaggtcgctg acctcggcgt ggacatcgcg ccgcgtctga aggtcctgca gacagccgag
     1261 ccgccggccc gtaaggcagg ggtgaaggtg tccagcgtgg cggagctggt cgagaagctc
     1321 aagacagaag cgggggttct ctaatggcag ttctggtcct tgcggagcac acgaacgaca
     1381 ccttcaatcc ggtgacggcg aaggcggtct cggccgcggc ggcactgggc ggtgaggtcc
     1441 acgttctcgt ggccgggtcg aactgccgtc cggcggcgga gggggctgcc aaaatcgaag
     1501 gcgtttctaa ggttctggtc gcggatgctc cccatctggc aaatcagctt gccgaggaga
     1561 ttgctgcact tatcgtgccg cttatggcca actatgacgc gctcgtggct ccttcgacgg
     1621 cccgcggcaa gaacgtgcta ccacgggtcg ccgccatgct cgacgtcatg cagatctccg
     1681 atatcacggc ggtgaagtcg cccgacacgt tcgagcggcc gatctacgcg gggaatgcca
     1741 ttcagaccgt gcaatcgacc gagccgaaga aggtcatcac ggtccgcacc gcagcgttcg
     1801 cggcagcgcc ggaaggggga tccgctacca tcgaatcgat ctcggcgccg gagcctgtcg
     1861 ggctgtcgac cttcgaaggt gccgagcttg ccaagtccga gcggccggag ctcaccgcgg
     1921 cccggatcgt catctcgggc gggcgcggcc tgcagtccgc ggagaacttc aagaagtaca
     1981 tagagccagt tgccgaccgg cttggcgctg cgatgggggc cagccgcgcc gccgtcgacg
     2041 ccggctttgc cccgaacgac ctgcaggtgg gccaaacggg taaggtcgtg gcaccggagc
     2101 tttacattgc ggtcggtatc tcgggagcca ttcagcacct tgcgggcatg aaggactcca
     2161 aggtgatcgt cgcgatcaac aaagatgggg aggccccgat cttccaggtc gctgattacg
     2221 ggctcgtcgc ggacctgttc caggctcttc ccgaactcga tgccgagctg cagaaggcag
     2281 gctattgaac ccggcggact atgctgatcg agtaccgttc aaaacgcagc tgctaaacga
     2341 gacagccaga tgccaggagc aaacaccgtg gccatcaaga aagtgggggt aatcggcgcc
     2401 ggccaaatgg gtagcgggat tgcccacgtg gttgcgctcg caggctatga cgtcctcata
     2461 acggacatct ccaaggaagt cgtcgacaaa gcgatggcga ccatcgggcg caacatggat
     2521 cggcagatcg ctcggggcat catcacggag gctgataagc aggctgcgct tgggcgtatc
     2581 gcatatgcgc cggattatga tgctttttcg gactgcgact tggtaatcga ggcggccacc
     2641 gaggacgaaa gcatcaaacg caagatcttc acggagctat ctccgaaggt caagcctgag
     2701 gcgatcatcg cttcaaacac atcctccatc tccattacgc gcctggcctc gatcacgaag
     2761 cggccggaac gcttcatcgg catgcatttc atgaatcccg tgccggtgat gcagctggtg
     2821 gagctgatcc gagggatcgc gacggaagat gataccttcg ccatctcgcg ggaattcgtg
     2881 gagtcactgg gtaagacgat tgccgtatcg gaggatttcc cggcattcat cgtcaaccgc
     2941 atcctgctgc cgatgatcaa cgaggccgtc tacaccctct atgagggcgt tggcaccgtg
     3001 gaggccatcg acaaggcgat gcggcttggc gccaatcacc cgatgggacc gctggagctg
     3061 gccgatttca tcggcctcga tacctgcctg tctgtgatgc aggtgcttta tgaagggttg
     3121 gcggattcga agtatcgccc gtgtccgctg ctggtgaagt acgttgaggc tggctggctc
     3181 ggccgcaaga cacagcgcgg tttctacgac taccggggcg agacgcccgt gccaacacgc
     3241 tagttggctg ctcctatccc atccctctga ccgtctgagg atgacgactg ctcctgggcg
     3301 aagtttgcca gagtgtcaat tgaccggcag cctctcgcga cagcggcacc gctcctagtc
     3361 gcttcattcc gcagctcctt agtcgggatc tggtcaaagg accgaacgag ggaaaccaaa
     3421 aatagaaatc gtcgtcggaa ttccctgcac ccaactgcaa ggggacgtaa cgcctattgg
     3481 cttttcaagc agagatcagg ctgtttgccc aaggctggta tggcgctgct gcgtcacgat
     3541 agttaattaa gaactctcga aagcttgcga atcaagggcc tgggcgttcg ggcaagaggc
     3601 aggagttcat gacgagcgat ctcgatcaag cctaccggcc atgcgtagg
//
LOCUS       DASUMG010000572         4042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_79527, whole genome shotgun sequence.
ACCESSION   DASUMG010000572 DASUMG010000000
VERSION     DASUMG010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79527"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            369..953
                     /locus_tag="VFX46_09575"
     CDS             369..953
                     /locus_tag="VFX46_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEX5600875.1"
                     /translation="MRSEELSVGLARTSGKSPGAPRPGQLYVCPLSRVHDTIAATGAR
                     YLVTLINLQTMLETPPGIDPANHLKIAVNDIIAPQDGLIHPCEDHVLALLRFAQIWNR
                     QGPLIVHCWAGISRSTAAAFITLCTLNPSVPEVLVARRIREASATAFPNRRLVELADG
                     LLGRGGRMLRAIDEMGPPKLAIEAEPFALPSTFP"
     gene            1096..2145
                     /locus_tag="VFX46_09580"
     CDS             1096..2145
                     /locus_tag="VFX46_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD regulator"
                     /protein_id="HEX5600876.1"
                     /translation="MTFEAHLKRTARAQTRESSVRERHEVAAVEIGLNAAILAVQKDE
                     PVVLVVRSQDETGQVIEALPFGPFSPLEHRTLEIGLRKWVSEQTGLELGYVEQLYTFG
                     DRGRHAQPGDVGPHVVSIGYLALTRPAKVGKSERWDWRSWYDYFPWEDWRRGKPAILT
                     EVIEPKLTTWAERPPVTPPPPGRPAQRSERLRMFFGLFGGTWDEEKVLERFELLYEAG
                     LVEEARRDGREAAATWDSLPRLGVPMRYDHRRILATAIGRIRAKIKYRPVVFELMPDE
                     FTLFELQKTVEAILGPHLHKQNFRRLVESAGLVEPTGEVRAKTGGRPARLYRFRREVL
                     LERPAPGVRVKPGRN"
     gene            2368..3426
                     /gene="nadA"
                     /locus_tag="VFX46_09585"
     CDS             2368..3426
                     /gene="nadA"
                     /locus_tag="VFX46_09585"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX5600877.1"
                     /translation="MATVQSETGVSALKGRNVLPMPDLKFTPELAREMEPLYARVSKV
                     VTPLEWPQFAPIIKAINELKKQRNAVILAHNYMTPEIFHCVGDFRGDSLQLAKEAART
                     DADVIVQAGVHFMAETSKILNPDKTVLIPDLRAGCSLASSITAEDVRMLREAYPGVPV
                     VTYVNTSAEVKAESDICCTSANAVKVVESLGVPRVIMIPDEYLAKYVQTKTSVEIITW
                     KGHCEVHERFTAEELEAFRAADPAVKIIAHPECPPDVIAAADFTGSTSGMIDWVKKNK
                     PAKVVLVTECSMSSNVAAEAPETEFIRPCNLCPHMKRITLQNILETLVYMRHEVTVDP
                     LVAERARRAVERMINLTN"
     gene            3481..>4042
                     /locus_tag="VFX46_09590"
     CDS             3481..>4042
                     /locus_tag="VFX46_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX5600878.1"
                     /translation="MAFGGRVKAPAGGGDIIIVGAGLAGLFTALKLAPAPVTLISPAP
                     LGEGASSAWAQGGIAAAVGEGDTPEQHAADTINAGAGLVDERIAHLVASEAADRVRDL
                     LAYGVPFDKDLGGRLILSREAAHSASRIVRVSGDKAGFAIMQAMIEAVRRTRSITVLE
                     NFSALSLIKTGGRVTGVTLTRPDDAAA"
BASE COUNT          762 a         1273 c         1269 g          738 t
ORIGIN      
        1 cgcggttgag cccggatttg gcagctatgt cgaaacctct atctcaccgc ggggtgggcc
       61 gatgagcggg ccgttcgctc cacacaatcg gcctggcttg gcaacaggtg gtttttcggc
      121 cgacgcggga tcaccctttg aaaatggcgc caatctgaga acaaccggca gcaaatcgag
      181 aaacgactga cgcgcggcga caggtcgcga tagtccctgc ccgagcgcag ctcagggcgg
      241 aaaatcatat cgttgtgagt tcttcccaac cgcttgcaaa cttagctttt tcgcttcatt
      301 tccaatttgc cggatttgag cgggttcgca cgcgttcgta acccgcattc gtttcgggca
      361 ttttagcgat gagaagtgag gagctctccg tcgggttggc tcgcaccagc ggcaagagcc
      421 ccggagcgcc gcgcccaggt cagctctacg tctgcccctt gagccgagtt cacgacacga
      481 ttgccgcaac cggcgctcgc taccttgtta cgttgatcaa tcttcaaacg atgctggaga
      541 cgccaccggg catcgatcca gccaatcatc tcaagatcgc cgtcaacgat atcatcgccc
      601 cgcaggacgg tctgatccat ccttgcgagg accacgtgct ggcgctgctt cgattcgcgc
      661 agatctggaa caggcaggga ccacttatcg tacattgctg ggccggtatc agccgctcga
      721 ccgctgctgc gttcatcacg ctctgcacgt tgaacccttc agtgcctgaa gtgcttgtgg
      781 cgcggcgcat acgggaagcc tcggcgacag cctttccgaa tcggcgtctc gtcgagctag
      841 ctgacgggtt gctgggccgg ggtgggcgaa tgctcagggc catcgacgaa atggggccgc
      901 caaagctggc cattgaagcc gagccgttcg cgcttccatc gacatttccg tgagccgcgc
      961 ggctcggctc aaccgccgtt cccgaaaggg atagtggccg ctcgcccaca gccgttgaac
     1021 ggaatgtgtg gccatacgga cggatccgaa attcgccatc atccgccagt actgccgcgg
     1081 ccaaggagat cgtggatgac cttcgaagct cacctgaagc ggaccgctcg agcgcagacg
     1141 cgcgagagca gtgtacggga gcggcacgag gtggcggctg tcgagatcgg cctcaatgca
     1201 gcgatcctcg ccgtgcagaa ggacgagccc gtcgtcctgg tcgtgcgttc gcaagacgaa
     1261 accggtcagg tcatcgaggc gctgccgttc ggtcccttct ctcctctcga gcaccggact
     1321 ctggaaatcg gcctgcgaaa gtgggtctcc gagcaaaccg gactcgagct tggatatgtc
     1381 gagcagctct atacattcgg cgaccgcggc cgccatgccc agccgggtga tgttggcccg
     1441 catgtcgttt caatcggata cctggcgctc acacgcccgg ccaaggtcgg caagtccgag
     1501 cggtgggatt ggcggagctg gtacgactat ttcccctggg aggactggcg gcggggcaag
     1561 ccggcaattc tgaccgaggt gattgagcct aaactcacca cgtgggccga gcgtccgccc
     1621 gtgacgccac ctcctcccgg ccgaccggcg cagcgctcgg agcgcctgcg catgttcttc
     1681 ggactgttcg gcggcacctg ggacgaagag aaggtgctgg agcgcttcga gctgctctat
     1741 gaagccgggc tcgttgagga agcacgacgc gacggtcgtg aggccgcagc gacgtgggac
     1801 agcctgccac gtcttggcgt gccgatgcgc tacgaccacc gccgtatcct ggcgacggcc
     1861 attgggcgaa tcagagcgaa gatcaagtat cgcccggttg tcttcgagct catgccggac
     1921 gagttcacgc tgttcgagct gcagaagacc gtcgaggcga ttctgggccc gcatctgcac
     1981 aagcagaact tccgcaggct ggtcgaaagt gctgggcttg tcgagccgac cggggaggtg
     2041 cgggcgaaga ctggcggacg ccctgcccgc ctctacaggt tccgccgcga ggtcctgctc
     2101 gagcggcccg caccgggcgt gcgcgtgaaa cccggccgca actgatcgat ggatccaggc
     2161 ccctcaccgg ggcctgcgtt cgacgcaaac cagtaatccg gagtcagacg aacgcaggtc
     2221 tggaaatgaa gggccatctg tcatatcttt agtttcacga ggaatgctca gaaggagcat
     2281 atctgacccg gaattttccg gaattttttc atgccacggt aatgctcaat ctgagcattc
     2341 tgtgagaagc acgagggagc gtcccccatg gccacggttc aatccgaaac gggcgtgtcg
     2401 gctctcaagg gtcgcaacgt cctcccgatg ccggacctaa aattcacgcc cgagctggct
     2461 cgcgagatgg agcccctcta cgcgcgggtt tcgaaggtcg tcacgccgct cgagtggccg
     2521 cagttcgcgc cgatcatcaa ggccatcaat gagctcaaga agcagcgcaa cgcggtgatc
     2581 ctggcccaca actacatgac gccggagatc ttccactgcg tgggcgattt ccgtggcgac
     2641 agccttcagc tggcaaagga agcggcccgc accgatgccg atgtgatcgt gcaggcgggc
     2701 gtgcacttca tggcggagac ctcgaagatt ctcaatcccg acaagacggt cctcatccca
     2761 gatctcaggg ccggctgctc attggcctcc tccatcacag ccgaggatgt gcgcatgctg
     2821 cgcgaggcct atcccggcgt gccggtcgtc acgtacgtca atacgtcggc ggaggttaag
     2881 gcggagagcg atatctgctg cacgtccgcc aacgccgtga aggtcgtcga gagccttggc
     2941 gtgccgcgcg tcatcatgat ccccgacgag tacctggcaa agtacgtgca gacgaagacc
     3001 agcgtcgaga tcatcacctg gaagggccac tgcgaggtcc atgagcgctt cacggcggag
     3061 gagcttgagg ctttccgtgc ggccgacccg gccgtgaaga tcatcgcaca tcccgagtgc
     3121 ccgcctgacg tcatcgcagc cgcggacttc accggctcga cctccggcat gatcgattgg
     3181 gtgaagaaga acaagccggc taaggtggtg ctggtcaccg agtgctcgat gagctccaac
     3241 gttgcggccg aggcaccgga gacggagttc atcaggccct gcaacctgtg cccgcatatg
     3301 aagcggatca cgctgcagaa catcctcgaa actctcgtct acatgcgcca cgaggtgacg
     3361 gttgacccgc tcgtggcgga gcgcgcccgg cgtgccgtcg agcgcatgat caacctgaca
     3421 aactgagcgc aaccgagccg ctgcaccctc aaacggcgca gcggctcagg attcatttcc
     3481 atggcatttg gcgggcgagt gaaggcacca gccgggggcg gcgatatcat tatcgtcggc
     3541 gcgggtctgg ccggtctgtt caccgcactg aagctggccc cggcgcccgt aactctcatc
     3601 tcacccgcgc ccctcggtga aggtgcctcc agcgcgtggg cgcagggcgg catcgcggcg
     3661 gctgttggcg aaggcgacac gccggagcag cacgctgcgg acacgatcaa cgctggcgcg
     3721 ggcctcgtcg acgagcggat tgcgcatctt gttgcatcgg aagcggcaga tcgggtccgc
     3781 gatctgctgg cctacggtgt cccgttcgac aaggatctcg gaggccgcct gatcctctcg
     3841 cgcgaggccg cccactctgc cagccgcatc gttcgcgtgt ctggcgacaa agccgggttt
     3901 gccatcatgc aagccatgat cgaggcggtc cgccgcacgc gttcgatcac cgttctcgag
     3961 aatttcagcg cgctttcgct gatcaagacc gggggccgcg ttaccggcgt caccctgacc
     4021 cgcccagacg acgccgctgc ta
//
LOCUS       DASUMG010000573         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_79680, whole genome shotgun sequence.
ACCESSION   DASUMG010000573 DASUMG010000000
VERSION     DASUMG010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79680"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VFX46_09595"
     CDS             complement(<1..842)
                     /locus_tag="VFX46_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HEX5600879.1"
                     /translation="MSDTILQAAALVVDPYVLWVCFGAAIFGIFVGAMPGLTATMATA
                     LLVPITFFMEPVPAVGAIVTATAMAIFAGDIPGAMLRIPGTPASAAYTEEAYRLSRKG
                     LLGQTLGVNLVFSVAGGLVGVAILIVAAPVLAEIALNFSSFEYFWLAGLGLTCAIFIA
                     QSDALKGFTALLIGLAIATIGIDPTAGHPRFTFGSVEMLQGISFIPAMIGLFALSELL
                     RGVVTIDQVGEMLQQQVGNIFRGIGQVWRRYWRNFVRGSMIGTGIGALPGAGADIAAW
                     ISFAV"
     gene            complement(855..1328)
                     /locus_tag="VFX46_09600"
     CDS             complement(855..1328)
                     /locus_tag="VFX46_09600"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HEX5600880.1"
                     /translation="MRANDTISGLVLIAVSTFMIYLTLDFPGFPGQKYGPALFPRLLG
                     MGLIICGLLLVVRGISARRAGEAWVAFADWTREPWRVTSFLLVPLLVVVYILVSEQVG
                     FIPVAFALLLLLFLWFRARIVVALPVAAIATWLIHWFFATLMRVPLPRGLLTNIL"
     gene            complement(1506..2501)
                     /locus_tag="VFX46_09605"
     CDS             complement(1506..2501)
                     /locus_tag="VFX46_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HEX5600881.1"
                     /translation="MSHFNRRQILAAGAATGAAAALGIKPSYAQADYPSRPVTVICPW
                     GAGGGTDATARIIAALLEKDLGQPFNVVNRTGGSGVVGHSAIATAQPDGYTLGMVTVE
                     ISMMHHQGLTELTPRDYTPLALMNEDPPGIQVKADSPFKDVKELADAIKSNPPGTFKA
                     SGTGQGGIWHLALIGWLLAMDLKPDHVPWVPSNGAAPAMQDLAAGGVDIVTCSVPEAK
                     AMLDAGKARSLAVMASKRNEAFPDVPTLNEVAGIDYSVGAWRGIVGPKGLPNEIATKL
                     TAALKKVYDSKEFKDFMTSRGFGMVWGDAEQFAAFMDESDAKMGTAMKAAGLAKA"
     gene            2798..>3309
                     /locus_tag="VFX46_09610"
     CDS             2798..>3309
                     /locus_tag="VFX46_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600882.1"
                     /translation="MRKPRSESHGPRFHGAVTRRGLLLGASASLLGLSASAVNAQATE
                     ARWWESIFDPQVERRRRRPPSREPSVPVEPNDLRPDATPWRSEVMLRQMDDAIARYQH
                     IVSQGGWHQIPQGPVVRPGEDDKRVPYVRRRLIIEGDLGPTDRRQDLSYTFDARMEHG
                     LREFQRRYGL"
BASE COUNT          696 a          997 c         1045 g          571 t
ORIGIN      
        1 accgcaaatg aaatccaggc tgcaatatcg gcaccggccc ccggtagcgc gccgatccca
       61 gtgccgatca tcgagccacg gacgaagttg cgccagtaac gccgccacac ctgaccgatc
      121 ccgcggaaga tattgccgac ctgctgctga agcatttcac caacttggtc tatggtgacg
      181 acgccgcgca gcagctcgga gagggcaaac aagccgatca tggctgggat gaagctgatg
      241 ccctggagca tctcaaccga gccgaaggtg aagcgcgggt ggccggctgt aggatcgatt
      301 ccgatggtcg cgatagccag gccgatgagc agggcagtaa agcctttcaa cgcgtccgat
      361 tgggctatga agatggcgca cgtcagcccc aggccggcga gccagaaata ttcaaacgag
      421 gagaagttga gggcaatttc agcgaggacg ggagctgcaa caatcaggat tgcgacacct
      481 acgagaccgc ccgccacgga gaagacgagg ttcaccccga gggtctgacc gagcaggccc
      541 ttgcgggaca acctataggc ctcttcggtg taagccgcag aagccggtgt gccggggatg
      601 cgcagcattg cacctggaat atcgccggcg aagatcgcca tggctgtcgc cgttacgatc
      661 gcaccaaccg caggcacagg ctccatgaag aaggtgatcg gtacgagcag ggcagttgcc
      721 atcgtggccg taagccccgg catcgcgccg acgaagatgc cgaaaatcgc tgcgccgaag
      781 cacacccaaa ggacgtatgg gtcaacgaca agggcggcgg cctggaggat tgtgtccgac
      841 atgcgcccgt gtccctaaag aatgttggtc agcagaccgc gtggaagcgg tacgcgcatc
      901 aaagtggcga agaaccaatg aatgagccag gttgcgatag cagcaacggg cagtgccacc
      961 acgatccgcg cgcgaaacca gaggaacagc aggagaagca gcgcaaatgc gactggaatg
     1021 aagccgactt gctccgaaac gaggatgtag acaacgacca gtagcggaac caggagaaag
     1081 gaggttaccc gccacggctc cctggtccag tcggcgaaag cgacccaggc ttcgccggca
     1141 cggcgcgccg agatcccgcg aacaacgaga agcaggccgc agataatgag gcccatgccc
     1201 agaaggcgcg ggaacagggc ggggccgtat ttttggccag gaaagccggg gaaatcgagg
     1261 gtcaggtaaa tcatgaaagt gctgacggcg atcagcacta ggcccgagat cgtgtcgttc
     1321 gctcgcatga ctgtgccggc ttcgagtagg tgtggcctgg aatgcggcct ccggagcaga
     1381 cacgacactc gctctcatcc tgaaagcagg aggcgagagg gacttccgat gtctcttcac
     1441 cctctcgcca tgagtggctt ttcagcgatc agaacaagtg cgtgatatgc gcgatctaag
     1501 cagcgtcagg ctttagccaa tcctgccgcc ttcatggcgg tgcccatctt cgcgtcgctc
     1561 tcgtccataa aggcagcgaa ctgctccgcg tcaccccaga ccatgccgaa gccgcgggag
     1621 gtcatgaaat ccttgaactc cttggagtcg tagaccttct tcagggcagc cgtcagcttc
     1681 gtcgcgatct cgtttggcaa ccccttcggc ccaacgatac cgcgccaagc gccgaccgag
     1741 tagtcgatcc cggccacttc attcaaggtc gggacatccg ggaacgcttc gttccgcttg
     1801 gacgccatca cggcgagtga tcgcgccttg ccggcgtcga gcatcgcctt ggcctcggga
     1861 accgagcacg tcacgatgtc cacgccacca gcggccaggt cctgcattgc aggcgcggca
     1921 ccgttcgagg gcacccaggg cacgtgatcc ggcttgagat ccatcgccaa gagccagccg
     1981 ataagagcca gatgccagat gccgccctgt ccggtgccag aggccttgaa ggtgcccgga
     2041 gggttcgact tgatggcgtc cgccagctcc ttgacgtctt tgaacgggct gtccgccttc
     2101 acctgaatgc ctggcggatc ctcgttcatc aacgccagtg gcgtgtagtc cctcggggtg
     2161 agctccgtca ggccctggtg atgcatcatg gagatctcga ctgtgaccat ccccagggta
     2221 tagccgtcag gctgtgcggt tgcgatcgcc gagtgtccta cgacgccgga gccacctgtg
     2281 cgattcacca cgttgaatgg ttgccccaga tctttctcga ggagcgccgc aatgatgcgc
     2341 gccgttgcgt ccgttccgcc accggcgccc cacgggcaga tcaccgtgac cggtcgcgac
     2401 ggataatcgg cttgcgcata agagggcttg attccgagcg ctgctgcagc accggtggca
     2461 gcgccggcag ccaggatctg gcggcgattg aaatggctca ttgagcttcc tcccagtcag
     2521 tcgagttttg aagtctttag gagtcccgac gtcggagcta cggtacgtga gcgtcggagc
     2581 tttgacaagt taacgtatcc attatgctcc ctgctcggga ggccgcgatg cctgccccgg
     2641 ctgcataggg atttcgcctc ggcgcggcat gagctatttc gatcaacagc ctttgtttgg
     2701 ccaaatctct tacgacaagg taacaagtcc gcctcaacag gaccgctgtt tcccgtcggc
     2761 ggtctgatca tgagcagcga aacagtcgag tttgccgatg cggaaaccga gatccgaaag
     2821 tcatgggccg cgtttccacg gcgcggttac acgccgtggc ctcctgctgg gtgcgagcgc
     2881 aagcctcctg gggctgagcg catcggcggt caatgcgcag gccacggaag cccggtggtg
     2941 ggagagcatc ttcgatcccc aagtcgaaag acgcagacgc cgcccgccga gccgcgagcc
     3001 ttctgttcca gtcgagccga acgacctcag gccggacgca acgccctggc gcagcgaggt
     3061 catgctgagg cagatggacg atgcgatcgc gcgctaccag cacatcgtgt ctcagggtgg
     3121 gtggcatcag ataccgcagg gtcctgtggt gcggccgggc gaagacgaca agcgggtgcc
     3181 ctacgtccgc cgtcgcttga ttatcgaggg cgatctcggg ccgacggacc gacgtcaaga
     3241 tctcagctat accttcgacg cccggatgga gcatgggctg agggagttcc agcggcggta
     3301 tggcctgag
//
LOCUS       DASUMG010000574         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_320892, whole genome shotgun sequence.
ACCESSION   DASUMG010000574 DASUMG010000000
VERSION     DASUMG010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320892"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1061
                     /gene="cysQ"
                     /locus_tag="VFX46_09615"
     CDS             234..1061
                     /gene="cysQ"
                     /locus_tag="VFX46_09615"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX5600883.1"
                     /translation="MDLDPNRLANLMLAPALAAGAAIMRHRRTGFTVERKTDSSPVTA
                     ADREAEEIILEALARYAPGVPVIAEEAVSRGERPRLDDAFFLVDPLDGTREFVSGGTD
                     FTVNIGLIVERTPRFGLIYAPASDQLFVTVGSDEAVEIRVSPEAGPVSLDDVQTTPIR
                     TREPDPARLVAVASRSHRTPDTDRFLADSRIASCRNIGSSLKFCLVARGDADIYPRFG
                     QTSEWDTAAGHAIVLAAGGLVTTLDKHPLEYGKSESGFTNPSFVVWGRSSLLPLIRL"
     gene            1162..2127
                     /locus_tag="VFX46_09620"
     CDS             1162..2127
                     /locus_tag="VFX46_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HEX5600884.1"
                     /translation="MLAGRSLFSKAKALAVAVALGAVLGGAEFGQAQDRLPWETPPKA
                     SSGYDTYAARNDAYRPDDSYRGGSGASGDAYRADDAYRPNDAYRPNGNAYRSGGDYYD
                     PNTDTYVPPPPGAGLGEGPAYTPYPDSQPEPYERDYGPPPREYDRYAEPPPPDYDHYD
                     DRTYSRNEILEAGHAFFGSVSRGLAAVVEYAFQKAGRPNGYILGEDAGGAFIAGLRYG
                     EGTLYTKDAGRHKVYWQGPSIGYDFGGEGSKTMVLVYNLRHPSEIYNRFAGVQGSAYV
                     VGGVSIQFQKHGHVVLAPIRSGVGLRLGANVGYLKYTRTPTWNPF"
     gene            2261..>3498
                     /locus_tag="VFX46_09625"
     CDS             2261..>3498
                     /locus_tag="VFX46_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600885.1"
                     /translation="MITIESTMLVALGFLLATLIAVLLAPAYRARAMRLTYKHMRRVM
                     PLTESELLLDRDRLRAHYAVRIHELETKLEKLRLAAARQQVEVSKRDATISALESELA
                     KLRAELEENVNARRVLEQTVMDRLPAVEQRLAETRKLLEQRDRELAAITADTSKNARA
                     LDEVMQINAQQRAEIERLNAVIATRGLRTGRSVRDPRFENELALRSELEALRAKTREQ
                     ESLISRLQTLVTMPAQAARANGGGASPEQEAELDRLRRDLVEAESALKSIADQADGKE
                     LADETMRARLQSLENLVADKAAKIKRLEAALAVYEKGEGDGRAISLKDSKIAMKARIA
                     SLQAEADERAATIEKLRAELAAANERLALQAARFRDEVRRLRAGPAPAAMQKDEPKAA
                     ARRSLAQRITDVNPALAASL"
BASE COUNT          636 a         1103 c         1133 g          626 t
ORIGIN      
        1 cggagattgt cttattgctc atgcgcgggg aggcgctcac gcgggctgac tggcgccttg
       61 taaaatcgct tttgagtgtt gatacctgcg tcgccacatc gttgccaaga tcggatcgcc
      121 ccgctcgggc gggctgcccg catttcctgg ggattggatt ctggtgactt gagtggggca
      181 ccttcgaaga ccttgaaggt tcaccagtaa actgggagac gctgggacag ctcatggact
      241 tggatcccaa cagacttgcc aatctcatgc ttgcaccggc gctggcggcc ggggctgcca
      301 tcatgcgcca ccggcgcacg ggatttaccg tcgagaggaa gacggattcg agccccgtca
      361 cggccgctga ccgggaggct gaggaaatca tccttgaggc acttgcccgg tatgcccccg
      421 gcgttcccgt gattgccgag gaagccgtaa gccgcggcga acgccctcgc ctcgatgatg
      481 ctttttttct cgttgatccg ctcgacggca cgcgtgaatt cgtgtctggc ggcacggatt
      541 tcacagtgaa catcggtctt atcgttgagc ggacgccacg attcgggctg atctacgcac
      601 cagcttccga tcagcttttc gtcacagttg gttcggacga agcggtagag attcgagttt
      661 cgccggaggc tgggcctgtc agcctcgacg atgttcagac aaccccgatt cggacgcgcg
      721 aacctgaccc agcgcggctc gttgctgtcg caagccgatc gcatcgcaca cccgatacgg
      781 accgttttct ggccgattcg cggatcgcaa gctgccggaa catcggctcg tcgctcaagt
      841 tttgcctcgt agcccgcggc gacgccgaca tatatccgcg attcggccag acgagcgaat
      901 gggatacagc tgccggccat gcgatcgtgc tggcggcggg cggccttgtg acgacgctcg
      961 acaagcaccc gctcgagtac ggcaagtccg aaagcgggtt cacgaaccca tcgttcgtgg
     1021 tttgggggcg gtcgtctctc ttgccattga tccgtctttg atcggacgcg tcagccacac
     1081 tttggtaata gttttgagga atggttgacg gttgataatc cgtcggcccg cgtgtcggcg
     1141 cgattgatga cgagtggata catgctcgca ggacgctcgc tattttcgaa ggccaaggct
     1201 ctcgccgtcg ctgtcgctct gggagcagtg ctcggcgggg cggaattcgg ccaggctcag
     1261 gatcgtttgc cttgggaaac cccgccgaag gctagctcgg ggtacgacac ctatgccgcc
     1321 cgcaacgatg cctaccgccc tgatgacagc taccgtgggg gctctggcgc ctccggcgat
     1381 gcgtaccgcg ccgatgacgc gtaccgcccc aacgatgcct accggcctaa cggcaacgcc
     1441 tatcggtcag gtggcgatta ttacgacccc aacaccgaca cgtacgtgcc gccgccacca
     1501 ggggccggac tcggcgaggg cccggcatac actccgtacc cggactctca gccggagcct
     1561 tacgagcggg attatggccc gcctccacgc gagtatgacc ggtacgcgga gccccctcct
     1621 ccagattacg accactacga tgaccgcacc tactcgcgga acgagatcct ggaggcgggg
     1681 catgcgttct tcggctcagt gagcaggggg ctggccgccg ttgtcgagta cgccttccag
     1741 aaggcgggcc ggccgaacgg ctatattctc ggtgaggacg caggcggtgc cttcattgcc
     1801 ggcttgcgct acggcgaggg cacgctctac accaaggatg ccggccggca taaggtctac
     1861 tggcaaggcc cgtcgattgg ctacgacttt ggaggcgagg gctcgaaaac gatggttctc
     1921 gtctataacc tgcgccaccc gagcgagatc tacaaccgct tcgctggtgt tcaggggtcc
     1981 gcctatgtgg tgggcggcgt gagcatccag ttccagaagc acggtcacgt ggtgctcgcg
     2041 ccgattcgtt caggagttgg gcttcgccta ggtgcgaacg tcggctattt gaagtatacc
     2101 cgaactccga cctggaatcc cttctgatcc ggcgttagcc gatctgtgaa ggggaacgga
     2161 tcgggacgag tgagattgcg ttgtaacttc acgaggggcc ggtcgtcgga gtcggcccta
     2221 cctactgtgt gctgttagga ttgcgttcgg ggatgcctca gtgatcacga tcgagtcgac
     2281 catgctcgtg gcgctggggt tcctgcttgc caccctcatc gctgtactcc tggcgccagc
     2341 ttaccgcgca cgcgcgatgc gcttgaccta caagcacatg cgccgggtga tgccgctcac
     2401 ggaaagcgag ctgctgcttg atcgcgaccg gctccgcgcc cactacgccg ttcgcattca
     2461 cgagctggaa accaagctgg agaagctgcg cctggcggcc gcccggcagc aggtggaggt
     2521 cagcaagcgc gacgcgacca tctctgcgct ggaatccgag ctcgccaagc tccgggccga
     2581 gctggaagag aacgtcaacg cgcgccgcgt tctcgagcag accgtgatgg atcgtctgcc
     2641 cgccgtcgag cagcggcttg ccgagacgcg caagctgctc gaacagcggg atcgcgagct
     2701 ggcggcgatc acagccgaca cgagcaagaa tgcccgcgcg ctcgacgagg tcatgcagat
     2761 caatgcccag cagcgggccg agatcgagcg gctcaacgcg gtcatcgcca cgcgcgggct
     2821 gcgcaccggc cgcagtgtga gagatccgcg cttcgaaaac gagctggcgc tgaggtcgga
     2881 gctggaggca ctccgtgcca agacgcgcga gcaggagtcg ctgatttcac gcctgcagac
     2941 gctggtgacc atgcccgcgc aagcagcgcg tgcaaacggc ggcggcgcga gccccgagca
     3001 ggaggccgag ctcgaccgcc tgcgccgcga cctcgtggaa gccgagagcg ccctcaagtc
     3061 catcgccgat caagccgatg gcaaggagct tgccgatgag accatgaggg cgcgcctgca
     3121 atcgctcgaa aacctcgtcg ccgacaaggc ggcgaagatc aagcggctcg aggccgcgct
     3181 cgcggtgtac gagaagggcg aaggcgatgg ccgcgcgatc tcgctgaagg acagtaagat
     3241 cgcgatgaaa gcgcgtattg catcccttca ggccgaggcc gacgagcgcg ccgccaccat
     3301 cgagaagctg cgcgccgagc ttgccgcagc caatgagcgg cttgccttgc aggctgcccg
     3361 gttcagagac gaggttcgcc ggctgcgtgc cggccccgcg cccgcagcga tgcagaagga
     3421 cgagccgaag gccgcagccc ggcgctcgct cgcccagcgc atcaccgacg tcaaccccgc
     3481 actggccgca tcgctcag
//
LOCUS       DASUMG010000575         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Hyphomicrobiaceae bacterium isolate SMAG_U1565
            k141_963168, whole genome shotgun sequence.
ACCESSION   DASUMG010000575 DASUMG010000000
VERSION     DASUMG010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961707
            Sequence Read Archive: SRR25158528
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Hyphomicrobiaceae bacterium (soil metagenome)
  ORGANISM  Hyphomicrobiaceae bacterium
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Hyphomicrobiaceae.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 08:04:10
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,928
            CDSs (total)                      :: 1,912
            Genes (coding)                    :: 1,889
            CDSs (with protein)               :: 1,889
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 4 of 23
            Pseudo Genes (incomplete)         :: 19 of 23
            Pseudo Genes (internal stop)      :: 2 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Hyphomicrobiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963168"
                     /isolate="SMAG_U1565"
                     /isolation_source="soil"
                     /db_xref="taxon:2015799"
                     /environmental_sample
                     /country="China"
                     /lat_lon="36.08 N 120.3641667 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFX46_09630"
     CDS             complement(<1..741)
                     /locus_tag="VFX46_09630"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX5600886.1"
                     /translation="MDQRPFRTYIEFEKPIAELESKLQELKALADGENAVSLTEEIAK
                     LEQKAQQMLADTYAKLTPWQKTQVARHPDRPHTLDYIKGLIEDFTLLAGDRYFAEDRA
                     IVGGLGRFRGRSVMIIGHEKGSDTESRIRHNFGMARPEGYRKAVRLMNMADRFGLPVI
                     TLIDTAGAYPGIGAEERGQAEAIARSTDCCLSLGVPIIAVVIGEGGSGGAVAIATANR
                     ILMLEHAIYTVASPEAAASILWRDSARAI"
     gene            complement(924..1874)
                     /gene="xerD"
                     /locus_tag="VFX46_09635"
     CDS             complement(924..1874)
                     /gene="xerD"
                     /locus_tag="VFX46_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEX5600887.1"
                     /translation="MSSCKPLIDAFLEMLAAERGAATNTLEAYRRDLETFSRFLTGRK
                     TSLAEARSADITSYLKALAEQGMAASSRARHLSAVRQFYKFLLAEGRIAEDPTHGIQG
                     PKVGQPLPKTLSVAEVDRLITRARDRIAETSGRERFAALRLYCLIELLYATGMRVTEL
                     VSLPRSVLQGDSRVLTIKGKGGRERMVPLNAAARAALDRYLEVGQHEDDDGLAPMLKT
                     RWLFPSRSAEGHLTRQRLGQELKELALEAGIDPRRVSPHVLRHAFASHLLDRGADLRA
                     VQQLLGHADISTTQIYTHVLEERLRRLVLEHHPLASAKLS"
     gene            complement(1885..>2967)
                     /locus_tag="VFX46_09640"
     CDS             complement(1885..>2967)
                     /locus_tag="VFX46_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX5600888.1"
                     /translation="DLAREEGVDNAGLLDALLAVGAGKKPRPPRSGRITPAHYRLLQL
                     ADAVDPAEIAQRADPALLGSLVLDPSTAEGLRVAAAEAAARLNIIAPEEVAVIYRAQR
                     FAPPDLANPLASRSDPLFRRALLFQAAEAERHPVRRAQILRAFLDEARRTGFYMPALA
                     MAAPLVKGVPRSPDLAWFAETAIEAALAAGDHASVRAWWVSLGARTRGGSRLEHWLAL
                     SDIANAEFDAPRGLHLPAVEALALQGGFSAPVLHRLATVLDALDYQVPMELWHLASST
                     PQPNEGHLPETGVLTQLEEASSRREFARTLLLVMRTVGPNGPEGAHIIALGDAIRALR
                     RVGLEADARRLGFEALFSAWPRSASH"
BASE COUNT          552 a          930 c          969 g          516 t
ORIGIN      
        1 gatggcgcgt gccgagtcac gccacaaaat cgaggcagcc gcttccggcg atgccaccgt
       61 atagatggcg tgctccaaca tgagtatgcg atttgccgtg gcgatcgcga cggcgcctcc
      121 agagccacct tcgccgatga ccaccgcgat gatcggcaca cccagtgaca ggcaacaatc
      181 ggtggaccgc gcaatggctt ccgcctggcc gcgctcctca gcaccgatgc caggataggc
      241 gccggccgtg tcaatgaggg tgatcaccgg caatccaaac cggtcggcca tattcatcag
      301 ccgcacggcc ttgcgatagc cctcaggccg cgccatgccg aagttgtggc gaatgcggct
      361 ttcggtgtcg gaccccttct cgtggccgat gatcatgacg gagcggccgc gaaaccgccc
      421 gaggccaccg acgatggctc tatcctcggc aaaataacgg tcgccggcca ggagcgtgaa
      481 gtcctcaatt agccctttga tgtagtcgag cgtatgaggt cggtcgggat gccgcgccac
      541 ttgcgtcttc tgccaagggg taagcttggc atacgtatcg gccagcatct gctgggcctt
      601 ctgctcgagc ttcgcgatct cctcggtgag cgatacggca ttctcaccgt ccgccagggc
      661 cttcagctct tgaagcttgc tttccagctc cgcgatcggc ttttcgaact cgatgtacgt
      721 cctgaagggt cgttgatcca tgggtgacca aggttagccg cgcgtcggag cggcggtcag
      781 gaatatgagc agggctccac tcgggcggga caatcgccag cgtcccaccg gaaagtcaag
      841 gttacactaa ggcgcggcaa aagttggccg aaacggtctg tgcggccaag cgatcgacga
      901 ccttagccgg cctcgttgct tgctcaactc agcttggcgc tcgctaacgg gtggtgctcc
      961 aacaccagac ggcgcaggcg ctcttccagc acgtgcgtgt agatttgggt ggtggagatg
     1021 tcagcgtggc cgaggagctg ctgtacggcg cgcaggtcgg cgccgcggtc cagcaaatgg
     1081 ctggcgaacg catgacggag cacgtgcggg ctgacgcggc ggggatcgat gccggcctcc
     1141 agtgccagct ccttgagctc ctggccgagt cgctgccgcg tgagatgccc ctccgcgctc
     1201 cgcgagggga agagccaccg cgtcttcagc atcggcgcca gcccatcgtc gtcctcgtgc
     1261 tggcccactt caagatagcg gtcgagggcc gcgcgggctg cagcgttcag aggtaccatc
     1321 ctctcgcggc cacctttgcc cttaatggtg agcacgcgcg agtccccttg gaggacggaa
     1381 cgtggcagcg acacaagctc cgtaacgcgc atgccggtcg cataaagcag ttcgatcaga
     1441 cagtagaggc ggagcgcagc gaaccgctcg cggcccgagg tttccgcaat ccgatctcgg
     1501 gctctcgtga tgagccgatc gacctctgca accgaaagcg tctttggtag gggctgccca
     1561 accttcggcc cctgaatgcc gtgcgttgga tcctccgcga tacggccctc ggcaagcaaa
     1621 aatttgtaga attgacggac ggctgaaagg tgcctcgcgc gggacgaggc ggccatgcct
     1681 tgctcggcaa gtgccttgag atagctcgtg atgtcggcag atctggcctc ggcgagggat
     1741 gttttgcggc ccgtcagaaa cctcgaaaac gtctcgagat cccgccggta agcttcgagt
     1801 gtgttggtgg cagcgccccg ctcggcggcg agcatttcaa ggaaagcatc gatgagaggt
     1861 ttgcagcttg acattagatt ccgctcagtg gctcgcggat cgtggccaag cgctaaacag
     1921 ggcctcgaag cctagacggc gcgcatcagc ctcgagcccg acgcgcctca acgcccggat
     1981 ggcatcgccc aaggcgataa tatgggcgcc ttccggccca tttggcccca cggtgcgcat
     2041 cacaagcaac aatgtgcgcg cgaactcgcg ccggctagag gcctcctcga gctgggtcag
     2101 cacgccggtt tcgggcagat ggccctcgtt gggttgtggc gtgctgctcg ccaagtgcca
     2161 aagctccatt ggcacctggt agtcgagagc atccaaaact gtcgcgagcc tatgaagaac
     2221 cggtgcgctg aagccgccct ggagggcaag ggcctcgacg gctggcagat gcaatccgcg
     2281 gggcgcatcg aattcggcat ttgcaatatc ggaaagcgca agccagtgct ccaagcggct
     2341 gccgcctcgt gtacgggcgc cgagagaaac ccaccacgca cgcacgctag cgtgatcccc
     2401 agctgcgagt gccgcctcga tggccgtctc tgcgaaccat gcgaggtctg gcgaccgtgg
     2461 cactcctttg accagcgggg cagccatagc caacgcaggc atatagaagc cggtgcgacg
     2521 cgcctcatca aggaatgcgc ggaggatttg cgcccgccga accggatggc gttccgcctc
     2581 ggccgcctga aagagcaggg cacggcggaa cagaggatcg gatcgggacg ccagcggatt
     2641 ggcgagatcc ggcggcgcga acctctgtgc acggtaaatg accgccactt cttccggcgc
     2701 gatgatgttg agtcgggccg ctgcctcggc ggcggcgacg cgaagcccct cagcggtgct
     2761 cgggtcgagc accagggagc cgagcaaggc aggatcggca cgctgggcta tctcggccgg
     2821 gtcgacagcg tcagcgagtt gcaggagacg atagtgcgcc ggggtgatcc ggccactgcg
     2881 cggcgggcga ggctttttcc ctgcccccac cgcaagtagg gcatcgagga ggccggcatt
     2941 atcgacgccc tcctcgcggg caagatc
//
